LOCUS       DATDUN010000001         1299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_123, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000001 DATDUN010000000
VERSION     DATDUN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VJC18_00005"
     CDS             <1..476
                     /locus_tag="VJC18_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD43788.1"
                     /translation="EPTAADQGLCALFVGTSGLGKTHLARALGYRHCQQNKKVLTTTV
                     NNMLLSLTTAESTGTLRKTLDQYINPALLIIDEVGYVSLRELESNLIFQVISARYDRR
                     RSTVVTTNRPFGEWNQIFHNDAIAHAILDRLAERAEVFHVEGKSYRETHPQRLRS"
     gene            945..1229
                     /locus_tag="VJC18_00010"
     CDS             945..1229
                     /locus_tag="VJC18_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLD43789.1"
                     /translation="MQRKNHSAEFKAKVALMSLKEDMTLSQLASKYEVHPTMITKWRK
                     HLLANVMGVFLKKSENGANKEKLADELYKQIGQLKVENEWLKKKFDLVIR"
BASE COUNT          360 a          279 c          321 g          339 t
ORIGIN      
        1 ccgaacccac agcggccgat caagggctct gtgccttgtt tgtggggact tcggggttag
       61 gcaaaactca tctggctcgg gctctggggt atcgtcactg ccagcaaaac aaaaaagtgc
      121 tgacaaccac cgtcaacaac atgcttctta gtctcaccac tgcggaatcc accggaacgt
      181 tgagaaaaac tttggatcaa tacattaacc ccgcacttct gatcatagat gaggtgggct
      241 acgtgagctt gcgcgagctc gaaagcaatc tgatattcca agtcatctcc gcccgatatg
      301 atcgacgacg gtccaccgtt gttactacca atcggccgtt tggagaatgg aatcagatct
      361 ttcacaacga tgctattgcg catgccattc tggatcgtct cgccgaaaga gctgaagtgt
      421 ttcatgtgga gggaaaaagc tatcgagaaa ctcaccccca acgcctgcga tcctgacacc
      481 gcacagttac gcacatgtct gctgtccttc tcttgatgaa aaaagagaaa atgccgatgt
      541 ctgtttttaa aaaacgattt atcaggcaaa ggtgtatcca aggctatgat gagtcattga
      601 ctggtatgcc tcaaaccgac gtttctggta tactttggga tcggcggttg caagtaaata
      661 ggtattcaag gttttatgtt ttgtaagcgt gaattcaagg atgaatttgg ccttgggatt
      721 gatgcgtgtt tttgtgatga tgtcgttttg gtctcgtttt tcaaaccaga tgtaattgcg
      781 aaaatctagg tccttagatt tcactggggc tttgcattca atgagccagg cagggctctc
      841 atcatccacc atggtatagt ggctggccaa aactggacca tttttcggga aatttaagaa
      901 aggtggtttg accgaaaagc cgtggctcct ttaaggagtc aactatgcaa agaaagaacc
      961 atagtgccga gtttaaggca aaggtggcgt tgatgtcctt aaaagaggat atgacgctca
     1021 gtcagctggc ctcaaaatat gaggtccatc cgaccatgat cacaaagtgg cggaagcacc
     1081 tgctggcaaa cgttatggga gtctttttaa agaagtccga aaacggggcc aacaaggaaa
     1141 agttggccga tgagctttat aaacagatag gccaactcaa agtggaaaac gagtggctta
     1201 aaaaaaaatt tgatctcgtt atccgttgat cagaaacgct ctttgataaa cagtgatggc
     1261 aaatgtcctc attgtatcgt ctgtttgtct acgaggctg
//
LOCUS       DATDUN010000002         1104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_249106, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000002 DATDUN010000000
VERSION     DATDUN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249106"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1104
                     /locus_tag="VJC18_00015"
     CDS             <1..>1104
                     /locus_tag="VJC18_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLD43790.1"
                     /translation="RAIESPKLPGIQISQEYRRFYPNKELAGQLLGAVGYDAKALGGL
                     ELTYDQFLRSATTNKSVERDARGKFFSEPDQNLDTHHLYLTIDKNIQTFAETALKKTA
                     TKHQAKSGFALVLDVKTGEVLAMANYPGFNPNAYWKYPLDHWKNHAVIDVFEPGSTFK
                     TILMAAALQNRTTKPTDRFFCENGQYQVGKNTINDHSPYGWLDAKTILQVSSNIGVTK
                     IAQKLGRKKFYEFIKEIGFGKEVDVGVSGESAGAVRPYKSWRDIDFSNIAFGQGISVN
                     GLQMTRAYGAIANGGHLFKPFLVKEIVDNSGHSVHKTEPINEKEVLDPTIAGELKQML
                     HLVTQTGGTSTQAHVPGYLSGGKTGTAQKFDTRL"
BASE COUNT          309 a          311 c          272 g          212 t
ORIGIN      
        1 ccgcgccatt gagtccccca aactccccgg cattcaaata tcccaggagt accgacgctt
       61 ctaccccaac aaagaactgg ccggacaact gctgggcgct gtgggctacg acgcgaaggc
      121 cctgggcggg ttggaactca cctacgatca atttttgaga tcggccacca ccaacaaaag
      181 tgtcgaaagg gatgcgcggg gcaagttttt ttcggagcct gaccagaacc tggataccca
      241 tcacctgtac ctgaccatcg acaagaacat ccaaacgttc gcggaaacag ccctcaaaaa
      301 aacagccaca aaacaccagg ccaaatcggg attcgccttg gtcctcgacg tgaaaaccgg
      361 agaggtcctg gccatggcca attaccccgg cttcaacccc aatgcgtatt ggaaataccc
      421 tcttgatcac tggaaaaatc acgctgtcat cgatgtgttt gagcccggct ccacgttcaa
      481 gacgatcctc atggccgcgg cactgcaaaa ccggaccacc aaaccgaccg acaggttttt
      541 ttgcgaaaac ggtcagtacc aggtgggcaa aaacaccatc aatgaccaca gcccctatgg
      601 ttggcttgat gccaaaacca tattgcaggt gtcttcgaat atcggtgtga ccaagatcgc
      661 acaaaagttg ggacgaaaaa aattctacga attcatcaag gagatcgggt ttggtaaaga
      721 ggttgacgtt ggcgtgagcg gcgaatccgc tggcgccgtg cgtccctaca agagctggcg
      781 ggacattgac ttcagcaaca tcgctttcgg acaggggatc agcgtcaatg gattacaaat
      841 gaccagagct tacggcgcga ttgctaacgg tggccacctc ttcaagccct ttctggtcaa
      901 agaaattgtg gacaattctg gtcactccgt tcacaaaaca gaaccaatca atgaaaaaga
      961 ggtgctggat ccaacgatcg ccggtgaact caagcaaatg ttgcatctgg taacacaaac
     1021 cggaggcact tccacccaag cacatgtccc cgggtatctc tcgggaggta aaacggggac
     1081 agcccagaaa ttcgacaccc ggct
//
LOCUS       DATDUN010000003         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_244, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000003 DATDUN010000000
VERSION     DATDUN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..>2075
                     /locus_tag="VJC18_00020"
     CDS             48..>2075
                     /locus_tag="VJC18_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43791.1"
                     /translation="MSPVDQSPLAVFELVFVLIQSGQYQLLSEEKKDIESRVVGVLSR
                     NPKKKPRNQSIDDAFDVQEIWSGSSHVHAAYDDYQRIHPSFEQINWLFRLSERIADAG
                     DETSASNLRSLCATLIQKKRRLQNTSLRRRIHEKYGLTPFRQKDSFLQHVQKLGCSES
                     ALLPTTPALLKEVMALMNLPVELGGGWSGVIGYLREFPDSLIKTALCLRLSSETDILP
                     KPNDEEGDQFVALWQQSLDATLPLVEKNGFFEPDENGVFAKYDRRGITPPTIQRPFVG
                     SANTSTCHYFQTHWVAMLTLMPHRSERPQAVAKGSRTLVFNPQLQLLRLDSLAPNLAP
                     LDRTQNLKLVLRLVHDSRLIPLSVQVDWVAQHGDISDVNALTTQIVDTCTSLPPAFRS
                     AYIPLFLQLSLRALDLNHPGRADALYKQAHEISQALESLTVQDLQPLFFYCKASLTEQ
                     KFDLGTRLREIKSSWEVANLDYLTRRYQRVTHRESLHLAQEISRIAENESIFDALESS
                     AFRRTAETLDARGEHDDLVIRLYERAIQCRVREEYERILDFDWRLVKSIDLCGLTLKE
                     KDRLLTTYLKLTLQRAVGPSVQEMLDRIDFDDHTTLKHRERAQQLLQPFHALLDMVED
                     RGYLMTEESLWQALDDFQEHILRYAPTSLQPVLCGVLANIAILRNPAIVDPA"
BASE COUNT          524 a          566 c          491 g          494 t
ORIGIN      
        1 gtttgacaaa aagcggtaga gtcagtgtcg aagccgctct cagggacatg tcgccagtcg
       61 atcagtcgcc tttggctgtt ttcgaacttg tttttgtact gattcaatcg ggtcaatatc
      121 aactcctttc cgaagaaaaa aaggatatcg agagtcgtgt tgtcggtgtc ctgagtcgta
      181 accccaagaa gaaaccgcgc aatcaaagca tcgatgacgc tttcgatgtt caggaaatct
      241 ggtctggcag cagtcatgtc catgccgcgt atgacgacta tcaaagaatt cacccttctt
      301 ttgagcagat caactggtta tttcgcctga gcgaacgtat cgccgacgct ggtgatgaaa
      361 cctcagccag caacctcagg tcactttgcg ccacgttgat tcaaaaaaaa cgtcgactgc
      421 aaaacacatc gctacgtcga cgcatccatg aaaaatatgg cctcacaccc tttcgccaga
      481 aagacagctt tctgcagcat gtccaaaagc tgggttgttc cgaatcagcc cttttgccca
      541 ccactcccgc actgctcaaa gaagtcatgg ccctcatgaa cctgcccgtt gaattgggtg
      601 gtggctggtc gggtgtgatc ggttatctgc gggaattccc agacagtctc atcaagacag
      661 ccttgtgttt gaggttatcg tcggaaactg atatcctgcc aaaacccaac gatgaggagg
      721 gagaccagtt cgtcgccctc tggcaacaat cacttgacgc caccctgccc cttgttgaaa
      781 aaaatggttt ttttgagcct gatgaaaacg gggtttttgc caagtatgac aggcgtggca
      841 tcacaccacc aacgatacaa cgaccatttg tgggttctgc aaatacttca acctgccact
      901 acttccagac acactgggta gccatgctca ctctcatgcc ccacaggtct gaacgcccac
      961 aggctgtcgc gaagggatcc cgcaccctgg ttttcaaccc acaattacag ttattgcgtt
     1021 tggattccct cgcccccaat ctggcccctc tggatcgcac tcaaaactta aaacttgtac
     1081 ttcggctcgt acacgattca aggctcatac ccctttcggt acaagtagac tgggtcgcac
     1141 agcacgggga catttccgat gtgaatgccc tgaccactca aatcgttgat acctgcacct
     1201 ctttgcctcc agccttccgg tcagcctaca tccccttgtt cctgcaactt tctctcaggg
     1261 ccttggacct gaaccatcct gggcgtgctg atgccttgta caaacaggcc catgaaatat
     1321 cgcaagccct ggaatccctg acagtgcaag acctgcaacc tctctttttt tattgcaaag
     1381 catcattgac agaacaaaaa ttcgatctgg gcacccgttt acgtgaaatc aaaagcagtt
     1441 gggaagtggc caacctcgat tatctgacga ggcgttatca aagggtcacc cacagggaaa
     1501 gtctgcatct ggcacaggaa atatcccgga ttgccgaaaa tgaatccatt ttcgacgcac
     1561 tggaatcaag cgcctttcgc cggacagcgg aaacgcttga tgcgaggggg gaacatgacg
     1621 atttagtcat caggctatac gaaagggcca ttcaatgcag ggtacgcgaa gaatacgaac
     1681 gcattctgga ttttgattgg cgactggtta agtccatcga tctgtgtggg ctgactctga
     1741 aggagaagga tcgtctgctg acgacctatt tgaaattgac cctgcaaagg gcagtggggc
     1801 caagcgtcca agaaatgctt gacaggatcg attttgatga tcacacaacc ttgaaacacc
     1861 gggaacgggc gcaacaactc ctgcaaccct ttcatgctct gctggacatg gtggaagaca
     1921 ggggttatct catgaccgaa gaatccctct ggcaggcgct ggatgacttt caggaacaca
     1981 tactgcgcta tgccccgacg tccctgcaac ccgtattgtg tggcgtgttg gccaatattg
     2041 ccatcctgcg caatccggca atcgttgacc ccgca
//
LOCUS       DATDUN010000004         3189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_249251, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000004 DATDUN010000000
VERSION     DATDUN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249251"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VJC18_00025"
     CDS             complement(<1..809)
                     /locus_tag="VJC18_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HLD43792.1"
                     /translation="MKTHTAIIALFVCFFPLLALATDTQQVSFYVIQPGGDGSTEEAK
                     PYLLEFFDYLREQTGTSFDGLYINSKNEAVTALQNKGVQLAIVSPEFIATYRDRFNFI
                     SVLKTIPIYSSGPYEKYFIMTNKATNLIDLADQNTKVSLYTSGDYDGAFLNNVIFGEN
                     EMIKKISWQMIQSPDVLNIIKEIDAGKTNTFVLLTGYEFSVIQQLKRSNSLPSLQLVF
                     SSTELPSSHVVTLGSGNTSLLGTLKTALVNMPNSLKGNMILKHLRLKGFAE"
     gene            997..1584
                     /locus_tag="VJC18_00030"
     CDS             997..1584
                     /locus_tag="VJC18_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43793.1"
                     /translation="MSKSTLSEEGRQTFIIDLPIMILFGALFAVFSHRLRQGTVFADP
                     YLWHAVVFATVFNVAVVWAIRLYPDWMWMYFLKDSHNTPSEYVYLFVFLYYFPVIFGF
                     YLGRDLRRVSFLFWLFFMAGLIAVEAWLILKLFDRYAVLGTNEAYLSGKAISLFGPEN
                     PLSLVMNGAVGVMIVYFLVVAFFYRRSEKKKLAGR"
     gene            1619..2083
                     /locus_tag="VJC18_00035"
     CDS             1619..2083
                     /locus_tag="VJC18_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43794.1"
                     /translation="MVAKNEALPKRAGLILVAIGAALIVEPEGLRVTHQEQSLITRCH
                     ATVREFPTLFRRGFIVGLYLFDLSVLFWGYGFKRFVALEQKKQQEFLDRCLQSRSGII
                     RNMMAGIRGLVMISYFSHPDVWKYIGYDPNGHVEERRRTRDERTKDERTKKG"
     gene            2197..>3189
                     /locus_tag="VJC18_00040"
     CDS             2197..>3189
                     /locus_tag="VJC18_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLD43795.1"
                     /translation="MLHSFKNIQKNIELDCDVCVVGSGAGGAVVATELVEAGLSVVMI
                     EEGSFFQTKDFKVDDTVWSTAHLYRDGGAGVIFGNPNIMYAEGRCVGGSTTVNGAMCW
                     RTPEKVLKRWQWERNLKDLTLKNLEPFFERVEKKINATPILPEAVNRDSELLRSGAER
                     LGYRYHANIRSAKGCVGANMCITGCPLGAKQSTALAYIPNFLASRGELFTNCRVHKVL
                     TKEGEVAGVTGWFVDPETKKKKFKLRVRAKTVVVCGGAIQTPALLKKSGLRDRAGLLG
                     SNLLVHPNMKVVGIFDEDVRAWHGVNQGYQITEFHDEGILMGVNFAAPGLIALSM"
BASE COUNT          784 a          649 c          880 g          876 t
ORIGIN      
        1 tactctgcga atcccttgag ccgcaaatgt ttgaggatca tgtttccctt gagggagttg
       61 ggcatgttga cgagagcggt cttgagcgta cccagcaaag aggtgttgcc gctaccgagc
      121 gtcaccacat ggctactggg aagttccgtg gacgaaaaaa caagttgcaa cgagggcaga
      181 gagtttgatc gcttgagctg ttgaatcacc gaaaattcgt aacctgtcag cagaacaaaa
      241 gtattggttt ttccggcatc gatttccttg atgatattta acacatcggg tgattgtatc
      301 atctgccagg aaatcttttt gatcatttcg ttttcgccaa agatgacatt gtttagaaac
      361 gcgccatcgt aatcccccga ggtgtaaaga ctcacctttg tattttgatc tgccagatca
      421 atcaggttgg tcgctttatt ggtcatgatg aaatattttt catacgggcc cgatgaataa
      481 atgggaatgg ttttgagtac agaaatgaaa ttaaaacggt cccgataggt cgcaatgaac
      541 tcgggactga cgatggccaa ctgaacaccc ttgttctgca acgcagtgac cgcttcattt
      601 tttgagttga tgtagagacc atcaaaggag gttcccgtct gttccctgag gtaatcaaaa
      661 aattccagaa gatagggttt tgcctcttcc gtcgaaccgt caccaccggg ttgaataacg
      721 taaaaactca cttgttgtgt atccgtcgcg agtgccaaca gcggaaagaa acaaacgaaa
      781 agggcgatga tagcggtgtg tgttttcaag gcgcaaaata taggtctgct caaaaaaagg
      841 ttcaacctaa attcggcaga tgagctgcca ttttttgttt ttcttttttt attgaccaaa
      901 ctcacattcg aacctaaaac tcccccatcg tctacacaaa aaactgagaa gaaaaacgct
      961 taaggcgcat gcattcggtt atgttctttg gggagagtgt ctaaatcgac actctctgag
     1021 gagggtaggc agacatttat cattgattta cccatcatga tcctttttgg ggccttgttt
     1081 gccgtttttt cacacagact taggcaaggg actgtttttg ccgatccata cctgtggcac
     1141 gccgttgttt ttgctacagt ttttaatgtg gctgtcgttt gggctatcag gttgtatcct
     1201 gactggatgt ggatgtattt tttaaaagat agccacaata ccccgtctga atacgtgtat
     1261 ttgtttgtgt ttctctatta ttttcccgtc atttttggat tttatttggg gcgcgatttg
     1321 cgcagggtgt cctttttgtt ttggttgttt ttcatggcgg gattaattgc cgttgaggcc
     1381 tggctgatcc ttaagctctt tgatcgttat gccgttcttg ggaccaatga ggcatatctg
     1441 agtgggaaag cgatttcgct atttgggccc gagaatcccc tgtcgctagt catgaatggg
     1501 gccgtgggtg tgatgattgt gtacttcctc gttgtggcgt ttttttatcg caggagcgaa
     1561 aaaaagaaat tagcagggcg ctgatggact tgttgaggtg ccctaaaggg taatcatgat
     1621 ggttgctaaa aatgaggcac tgcccaaacg cgcgggattg atattggttg ccatcggtgc
     1681 ggccctgatc gtggaacccg aagggcttcg tgttacccat caagaacagt cgctcatcac
     1741 gagatgtcac gcgacggtca gggaatttcc aacgttattc aggcggggct ttattgtggg
     1801 gctgtatttg tttgacctga gtgtgttgtt ttggggttat ggttttaaac gttttgtggc
     1861 tttagagcaa aaaaaacagc aggaatttct ggatcggtgc ctgcaaagcc gctcggggat
     1921 cattaggaac atgatggcag ggatcagggg gctggtgatg atcagttatt tttcccaccc
     1981 cgatgtgtgg aagtatattg gctatgatcc aaacgggcac gttgaggaga gacgaaggac
     2041 gagggacgaa aggacgaagg acgaaaggac gaaaaaaggc tgacgaccta cggccaacag
     2101 acaacagcca gattctggcc gttgacagtg agctgttgac tgtaagccga gagccctgcg
     2161 tcatattgcg ccacagcgtt ttattggtgt gtcatcatgc ttcattcatt caaaaacatt
     2221 cagaaaaaca tcgaacttga ttgcgatgtt tgcgtcgtgg gctctggtgc gggcggtgcc
     2281 gtggtggcca cagaattggt tgaggcagga ctctctgtgg tgatgattga agaaggttct
     2341 ttttttcaaa ccaaagattt caaggtcgat gacacggtct ggtccacagc gcatttgtat
     2401 cgcgacggtg gggctggggt gatttttgga aatcccaaca tcatgtacgc agaaggccgc
     2461 tgtgtggggg ggagcaccac ggtgaacggg gccatgtgct ggcggacccc cgagaaggtc
     2521 ctcaaaagat ggcagtggga aagaaacctc aaagacctca ctctgaaaaa tctggaaccg
     2581 ttttttgaac gtgtcgaaaa aaaaatcaat gccacgccga ttttacccga agcggttaac
     2641 cgtgattcag agctgttgcg ctcgggggcc gagcgattgg gctatcgtta tcatgccaac
     2701 atacgatcag ccaagggttg tgtgggggcc aatatgtgca tcaccggctg ccctttgggt
     2761 gccaaacaat caacagcgtt ggcttatatt ccaaactttc ttgccagtcg cggggaattg
     2821 tttacaaatt gtcgtgtgca taaggtgttg accaaagagg gtgaggtggc tggcgtgaca
     2881 gggtggttcg tagaccctga aacgaaaaag aaaaaattta aactcagagt cagggcgaag
     2941 acggtcgttg tttgtggggg cgccatccaa accccggctt tgttgaaaaa aagtggcttg
     3001 cgtgatcggg cagggctgtt gggaagcaac ttgctggtcc accccaacat gaaggtggtg
     3061 gggatttttg atgaggatgt ccgggcctgg catggtgtga atcaggggta tcagatcacg
     3121 gaattccacg atgaagggat actgatgggg gtgaattttg cggcacccgg actcattgcc
     3181 ctttccatg
//
LOCUS       DATDUN010000005         2480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_41754, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000005 DATDUN010000000
VERSION     DATDUN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41754"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /locus_tag="VJC18_00045"
     CDS             <1..1863
                     /locus_tag="VJC18_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43796.1"
                     /translation="YSLQGTFRNRQWITSERSEEDYHALKTAYDSLKQIGISDDDCHI
                     LLEQVDKQYRGSLSDALRALLVIVAKSPQSNPNVILEFHKALCDRSLTEDSVPVLDVV
                     HLDALLDMFSYSQVDLATEANEFIAVAHVVRSSCGSRLKPLFAVLRIDWHDLLGQLGD
                     GVDVHNSHEVREKIRKVLAKEMAVHLNAYVAEADGDTRGYNEAIRDIARTLSHICEKE
                     KEDDQTFVFLEQFARHVSDSSFFELLTIGFGPMRVPIFDYLLLSRVTSRKNLSLVQWL
                     KEMDPDAQKVLDVMTALFSLNKTPLASGCADYFLLEGLEALTRTQDDLLEYAGVYVYF
                     CERYLGRLTQDNKRLFAQKLFKAYAQPNLTPEQKGVLAFLIKTYSSKQRLSVYETDLA
                     TVSRDLPDVKKLNIPVSSWLADGVLSARLMYYYNPLDGKNPDKSDMVWWFQKDLEMFK
                     TMQYELGPLVDDSATTSHVEVMANQGNVPVRILMTLQPVDAIKDDYARDEFDVMVQRG
                     HGDSVKMLFDTKAGGTGADFKDKLLITGACRAVGDTLTDDVQKYFINNYFMGDLGEGK
                     GFYNSLIPDQLFRAIMMGNDQWSALNEFRLEGFILPDHNIYRLAPYVFDYVKSR"
     gene            1955..>2480
                     /locus_tag="VJC18_00050"
     CDS             1955..>2480
                     /locus_tag="VJC18_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43797.1"
                     /translation="MNQNDKWAKNAQRSLAERLGFVKRGYETAKQFNLIQYALFWHAD
                     CMLIRPVCGDICRRKHMRGYMRHNIIRAMVIVLLLLWVFSNNARAELSNLSRIDTGGN
                     LNDIVIEGNYAYAIDFSTGLNIIDISDLTAPSLLGTYGYANPSGVAVSGNIAYGIGAY
                     GTSLQILDVSDPANP"
BASE COUNT          660 a          522 c          621 g          677 t
ORIGIN      
        1 tattcactgc aaggaacatt tcggaacaga caatggatta caagcgagcg tagcgaagaa
       61 gactatcacg cactcaagac cgcatacgat tccctgaagc aaattggtat atcagatgac
      121 gactgtcaca tattattaga gcaggtcgac aaacaatatc ggggctcctt gtctgatgct
      181 ctgagggccc ttttggtgat tgttgcaaaa tcaccccaaa gcaatccaaa tgtcattttg
      241 gaatttcata aggccctatg cgatcgttca ttgacagaag attctgtgcc tgtcctcgat
      301 gttgttcatt tggatgccct tttggacatg ttttcttatt cccaagtcga tctggcaact
      361 gaggcaaatg agtttattgc tgtggcccac gtggtgagga gcagttgcgg atcgcggctg
      421 aaaccccttt ttgctgtctt gaggattgat tggcatgacc tcttggggca actaggtgat
      481 ggggtggatg ttcacaatag tcatgaggtc agagaaaaaa tcagaaaagt cctggcaaaa
      541 gaaatggctg tgcatctcaa cgcgtatgtg gctgaggcag acggggatac ccgaggttac
      601 aatgaagcta ttcgcgacat cgcgaggacc ttgtcgcaca tttgcgaaaa agaaaaagag
      661 gatgatcaga cctttgtttt tctcgaacag tttgcgcggc acgtttcaga cagctcgttt
      721 tttgaattat taacgattgg gtttggcccc atgcgtgttc ccatatttga ctacctcctg
      781 ctttctcgcg tgacatcacg caaaaattta agcttggtcc aatggctcaa agaaatggat
      841 cctgacgcac aaaaagtgct ggatgtcatg acagccttgt tttcgctcaa taagacgccg
      901 ctggccagcg gttgtgccga ttatttttta ttggaggggc tggaggccct cacgcgcaca
      961 caggatgatc tattggaata tgctggggtg tatgtttatt tctgtgaacg ttatctgggc
     1021 aggctgactc aggacaacaa aaggttgttc gcccaaaaat tattcaaggc ctacgcacaa
     1081 cccaatctga ctcctgagca aaaaggagtc ttggcttttt tgatcaagac atattccagc
     1141 aaacaacgtc tgtcggttta cgaaacggat ctggcgactg tgagtcgtga tttgcccgat
     1201 gtgaaaaaac tgaacatccc tgtctcgagc tggttggccg atggcgtcct ttcggcgcga
     1261 ctgatgtatt attacaatcc actcgatggc aaaaatcccg acaagagcga catggtctgg
     1321 tggtttcaaa aagatctgga gatgttcaaa accatgcaat atgagttggg gcctctggtg
     1381 gatgattccg cgaccacctc acatgtggag gtgatggcca atcagggaaa tgttccggtt
     1441 cgcatcctca tgaccctcca gcctgttgat gccatcaaag atgactatgc tcgggatgag
     1501 tttgacgtga tggtgcaacg agggcatggg gattccgtca aaatgctatt cgatacaaag
     1561 gctgggggaa cgggggcaga ctttaaggat aaattgttga tcacaggggc ttgccgcgct
     1621 gtgggcgata cactgaccga tgatgtccag aaatatttta taaacaatta ttttatgggt
     1681 gatctgggtg aaggaaaagg tttttacaac agtctgatcc ctgaccagct cttccgcgcg
     1741 atcatgatgg gcaatgatca atggtcggcg ctcaatgaat ttagactgga aggttttatt
     1801 ttgccggatc acaacatcta tcgattggcc ccctatgtgt tcgattatgt gaagagtcgg
     1861 taaattctca ggcgtgtcca cgtccagaaa atccatttta taaaaaaaga aaacgttttt
     1921 ttccttccct tatagactgt ttgcactggc acctttgaat caaaacgata aatgggcaaa
     1981 aaatgcccag cgaagtctgg ctgaaaggct tggttttgta aaacgtggtt acgaaacggc
     2041 taaacaattc aatttgatac aatacgcatt attctggcac gcggattgca tgttaataag
     2101 acctgtgtgt ggggatattt gtcggcgaaa acatatgagg gggtatatgc gacacaacat
     2161 catacgagca atggtcattg tgctactact tctatgggtt ttcagcaata acgcacgtgc
     2221 ggagctttcc aatttaagcc gtattgatac tggtggaaac ttgaacgaca ttgtcattga
     2281 gggcaattat gcctatgcca tcgatttttc aacgggattg aatattattg acattagcga
     2341 tttaacggca ccatctctgc taggcacata cggatacgcg aatccgtctg gcgtcgcagt
     2401 cagtggtaac atagcctatg gaattggggc atatgggacc agtcttcaga tacttgacgt
     2461 gtctgatcca gctaacccct
//
LOCUS       DATDUN010000006         1076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_332427, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000006 DATDUN010000000
VERSION     DATDUN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332427"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VJC18_00055"
     CDS             <1..486
                     /locus_tag="VJC18_00055"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLD43798.1"
                     /translation="SLGGVLTLRLAQEHGDKISGIACLAAPAFLHKWVRATLPIVMRS
                     PLRFVYRYQPKAQADVKDPIARKNYWSVDQMPLKCIHSLTELQKKVRAELKTVNNPAL
                     LIHSRYDATAPYESLDYFAKNISSKVIETVTLENSFHLITIDYEKDTVSQKVGEFFGK
                     L"
     gene            complement(526..>1076)
                     /locus_tag="VJC18_00060"
     CDS             complement(526..>1076)
                     /locus_tag="VJC18_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43799.1"
                     /translation="INMSDKMQNYLSDLLKTMSPTLLMPQSQAVSLEKSGFGKEEKKS
                     GAEDHSHHNQLVIESADDSVSEVVLEEKPAQLYENFVKNGQVKMVVSKAQLLSMLDEV
                     QQTQKQSGTEAARQVPLMVVPTACQTIEDRKALAALVDTSLEEDAEADDQDQEEDAEE
                     DDEKKDDGSMTLKEFKTLYLKI"
BASE COUNT          249 a          291 c          247 g          289 t
ORIGIN      
        1 tcgttgggcg gagttctcac gctcaggctg gcgcaggagc acggcgacaa aatcagtggg
       61 atcgcgtgtc tggccgcacc ggcatttctg cacaagtggg tcagggccac cctccccatc
      121 gtgatgcgct cccctctgcg ctttgtgtac cggtaccagc ccaaggcgca ggccgatgtc
      181 aaggacccga tcgcgcgcaa aaattattgg agtgtggatc agatgcccct caagtgtatc
      241 cacagtctca ccgagctgca aaaaaaagta cgcgccgagt tgaagaccgt aaacaacccc
      301 gccctcctca ttcattcccg ttacgatgcc accgcaccat acgaaagtct ggattatttc
      361 gccaaaaaca tttcttcgaa agtcatcgaa accgtcacgc tggaaaacag ttttcacctc
      421 atcacgattg attacgaaaa agacaccgtg agccagaagg tgggagagtt ttttgggaaa
      481 ttataactcc ccccaacccc ctcatatgga gagggcgagg gggagttata tcttcaaata
      541 cagagttttg aattccttga gggtcatgga accatcatct tttttttcat cgtcttcttc
      601 ggcatcctct tcctgatcct gatcgtcagc ttcggcgtct tcttctaatg acgtatcaac
      661 gagagccgcc aatgcttttc tgtcttcaat ggtctggcag gccgttggca caaccatcag
      721 gggcacctgc cgtgccgctt cggtaccaga ttgtttttga gtctgttgca cctcatcgag
      781 catgctgagc agttgtgctt tggaaaccac cattttgacc tgtccatttt tgacaaaatt
      841 ctcataaagc tgtgcgggtt tttcttcgag cacaacctca ctcacagaat cgtccgctga
      901 ttcaatcacc agctgattgt gatgactgtg gtcttcggct ccagattttt tttcttcttt
      961 tccaaaacct gatttctcaa gactgacggc ctgagactgc ggcatcagca acgtgggtga
     1021 catggtcttt aacaaatcac tcagataatt ctgcatcttg tcggacatgt tgattt
//
LOCUS       DATDUN010000007         3025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_290976, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000007 DATDUN010000000
VERSION     DATDUN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290976"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VJC18_00065"
     CDS             <1..691
                     /locus_tag="VJC18_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HLD43800.1"
                     /translation="SLQTQKARRRLAVIQRFTELGSGFKVASHDLEIRGAGNILGDEQ
                     SGHIAAIGYDLYIQLLQEAIHELKNQHIPEDFEPEILLNIPAKIPADYVPDEHLRLLL
                     YKQLSSCRAFDEVASTREEWLDRFGKLPEETENLIGIIRIKMTCHHLLVRQIKQSGPA
                     LFFDFHPNHQIDTAVFTRAIQANGKQFSISRDGKFVINRLFCSPQELLAGVQDFLEKL
                     KTNSLRSEPFS"
     gene            1039..1203
                     /locus_tag="VJC18_00070"
     CDS             1039..1203
                     /locus_tag="VJC18_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLD43801.1"
                     /translation="MEKKISQEDLAKKLDVAFSTVNRWFNRRSQPSSIQRYYIRKMMG
                     RNCYLQDAKT"
     gene            1227..2378
                     /locus_tag="VJC18_00075"
     CDS             1227..2378
                     /locus_tag="VJC18_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004054825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HLD43802.1"
                     /translation="MKPKKQHRSQNIVSEGEAALYKRIRDLVINARKTVARGVNLVQA
                     HTNFEIGRYVVEHEQQGKGRAAYGEEVLKTLAERLTREFKKGFSLSNLKSMRQFYLQN
                     QNRIGQSITGQLGKALSGASLGGPSTKSQSLTGQLEPSVATHPFTLSWTHYVFLLGVK
                     NSDERRFYEIEATQQNWTVSELKRQFNSSLYERLALSRDKAGIKKLAKKGQLIEKPED
                     LLKEPLVLEFLGLDEQTRYSESDLEAAIINQIERFLLELGKGFLFEARQKRFSFDDEH
                     YFVDLVFYNRLLHCYVLADLKLGKLTHQDLGQMQMYVNYFDRFVKTENENSTIGIVLC
                     KKKHEALVKITLPKEANIHAREYQLYLPSKEMLQKKLVEWTEKAEEVWS"
     gene            2573..2800
                     /locus_tag="VJC18_00080"
                     /pseudo
     CDS             2573..2800
                     /locus_tag="VJC18_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014195555.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
BASE COUNT          902 a          651 c          727 g          745 t
ORIGIN      
        1 atcactgcaa acacaaaagg cccggcgccg cctggccgtg atccagcgtt ttacggaact
       61 gggcagtggg ttcaaggtgg cgtcacacga tctcgaaatt cgcggggcgg gcaacattct
      121 gggcgacgag cagtcgggac acatcgccgc catcggttat gacctgtaca tccaactcct
      181 gcaagaggcc attcacgaac tgaaaaacca gcacatccct gaggactttg aacccgaaat
      241 cctccttaac atccccgcca agattccggc agactacgtt cctgacgagc acctcaggtt
      301 gctcctgtac aaacagctca gctcctgcag agcttttgat gaagtggctt ctacccgaga
      361 agagtggctg gaccgttttg gaaaactgcc ggaagaaacg gagaacctca tcggcatcat
      421 ccgcatcaaa atgacttgtc accaccttct ggtccgtcag atcaagcaaa gtggccctgc
      481 tctctttttt gattttcacc ccaatcatca gatcgacacc gccgtcttta cgcgagccat
      541 acaggccaat ggcaaacagt tttcaatctc tcgcgatggc aagtttgtga tcaacagatt
      601 gttttgctcg ccacaggagc tgcttgctgg tgtgcaggat tttttggaga aactgaaaac
      661 gaactcactg aggtcagagc cgttctcgtg aaggtaaaaa caacaacgct gtgggcacga
      721 actgacatca acggcaacgc ggccaaacct ttttcggccg tcggaattaa atcaccaccc
      781 aaggctctta aaatcacaaa aactgttaaa atcctagtgg cacaaagtcg gagcgtacac
      841 acaacccatt aaaatataag acttttaatt ttcgaggtgt ctaagtctga agaaggtggt
      901 gtgtgaatag aaatgtttgt attttaattg ctttaggtca aaacgcgcta taacttgcag
      961 gaactatgag agttggcgca acaggactga tagatcgcct ggaggtgttt cgatccgcct
     1021 agggctcatg ccggtgattt ggaaaagaaa atttcacaag aggacctggc caaaaaattg
     1081 gacgtggctt tttctaccgt aaatcgttgg ttcaaccgca ggtcacagcc gagctcaatt
     1141 cagaggtatt atattaggaa gatgatgggg agaaactgtt acttgcaaga tgcaaaaact
     1201 tgacagatga agattggggg tttgctatga aaccaaaaaa acaacacaga tcgcaaaaca
     1261 ttgtgtctga aggcgaggcg gccctctata aacgcatacg cgatctggtg atcaatgctc
     1321 gcaaaacagt tgcccgaggc gttaatcttg tgcaggccca caccaatttt gaaattggac
     1381 ggtatgttgt tgaacatgag caacaaggca agggtagagc tgcttacgga gaagaggtgc
     1441 ttaaaacatt ggctgaacgg ctcacgagag aatttaaaaa aggcttttcg ctttccaacc
     1501 ttaaatcaat gaggcaattc tatttgcaaa atcagaatcg aattggccag tcaatcactg
     1561 gccaattggg caaggctctt tctggggcat cgcttggtgg gccatcaaca aaaagccagt
     1621 cactgactgg ccaattggag ccttctgttg caactcatcc cttcacgttg agttggacac
     1681 actacgtttt tcttttgggg gtcaaaaatt ctgacgagcg tagattttac gaaattgaag
     1741 caacccaaca aaactggacc gttagcgaac tcaaacgaca atttaacagt agtctttacg
     1801 aacgtcttgc cttaagtcgt gacaaggcgg gaataaagaa attggccaag aaagggcagt
     1861 tgatagaaaa accggaagac ttgctcaagg aaccccttgt gcttgagttt cttggtttgg
     1921 acgagcagac tcgctactcc gaatccgatc ttgaagctgc aattatcaat caaattgagc
     1981 gttttttgct cgaattgggc aaagggtttt tatttgaagc gcggcaaaag cgttttagtt
     2041 ttgatgatga acactatttt gttgatcttg ttttctacaa tcgcttgctc cattgttatg
     2101 tgctcgccga tttaaaactt ggcaagctta cccaccaaga tcttggtcaa atgcagatgt
     2161 acgtgaatta ttttgaccgg ttcgtcaaga cagagaatga aaattccacc attggcattg
     2221 tgctgtgcaa aaaaaagcat gaagcattgg tcaagatcac attgccaaag gaggccaata
     2281 tacatgcgcg ggagtatcaa ctttatctgc ccagcaaaga gatgttgcaa aaaaagttgg
     2341 tcgagtggac tgagaaagct gaagaggttt ggtcctaaaa aaggtggtta ttgggaggtg
     2401 gtgaagaaag gcaaaattaa atgagagtgg atgcaacaga actgatagac ctcactttct
     2461 ctaccatgaa ccgttggttc aaccgcagat cacagccaaa ccatgttcag cggcatcata
     2521 ttaggaagat gttggagcgc acaaacaatc ttaaaacggg ggaccaacgg gcatgattac
     2581 cccataccac gccaaatatt ttgcttacga acttacaaga cgttgtgctt ctaatggttt
     2641 agaaaaactg tcctcaactt tgtcgaatgc gcaagttgat ttaaaaccgc accaaataga
     2701 ggccgcgctg tttgcgtttc gttctccctt atccaaaggc gccatattgg ctgatgaagt
     2761 tgggttaggg aagaccatag aagcaggttt ggttatttca catgattgcg cagacagttg
     2821 tttgttgcgc aggacgatgt cgacaacaaa aaagaggagc taatcagtcg tgttgaaaag
     2881 caattacgac aaagagcatc gttgaaaaag atgttcacga tgaggtggtt attaaaatag
     2941 gctttcacaa aaggcgagat ggagcgttgt gtaaaaaaaa taatgacaaa gcacagaacg
     3001 tataacaagg ttgttggcac gatag
//
LOCUS       DATDUN010000008         1620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_208019, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000008 DATDUN010000000
VERSION     DATDUN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208019"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /gene="argS"
                     /locus_tag="VJC18_00085"
     CDS             complement(<1..491)
                     /gene="argS"
                     /locus_tag="VJC18_00085"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HLD43803.1"
                     /translation="MKTVFEIIKNEFCCAISQVAPLLLEVDPMIRLTTEGKFGDYQSN
                     VAMGLAKTLRRAPREVAQEIVQNLQLAQVCERVEIAGPGFINLFLSRDYLAGHLTRLS
                     QEARLGAEQAAEPVHHIIDYSSPNLAKEMHVGHLRSTIIGEVLARVIEFAGHTVDRVN
                     HVG"
     gene            complement(496..>1620)
                     /locus_tag="VJC18_00090"
     CDS             complement(496..>1620)
                     /locus_tag="VJC18_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLD43804.1"
                     /translation="QGLTPSELDAKLDMMAAQITPQAGQELSFLTLKCLQKDVTRALP
                     LFFDLLRQPRFDPERLQVIKKNKITAIQRRNESSLEIASREFLQGLYGPDSPYAWQYT
                     EQTIAPIDTKMLLDFHARHVAPRQLIIASTSPDPADEFWSRIKPHVAEWNHNVPDAVP
                     PKPIEKEWTPGWAVVQKDDSQSAIVMGHFGEKRFNPDKYKIILANEILGGSTFGSRLG
                     KRLRTELGLVYSLGSSFGLGTDYESFRISTRTNAAKTLTVVNEIKNIVSEMVNRHDIT
                     DGELAAAKERILNRLVFDFEQPFKIVTGRVLYDHYGYPPNYVALYQKKIEEVTLSEIN
                     AVLGTYFFPDRFRVMIVGDLARMPDLNDKESVVEIPLDWE"
BASE COUNT          376 a          472 c          380 g          392 t
ORIGIN      
        1 tcccccacat gattcacacg gtcaacggtg tgccccgcaa attctatcac cctcgcgagc
       61 acctcgccaa tgatcgtgct tctcaggtgc cccacgtgca tttccttggc caaattggga
      121 ctggaataat caatgatgtg atgcacaggc tcggcggcct gctctgcgcc cagacgagcc
      181 tcttgagaca aacgcgtcag gtgcccagcc agataatccc gactcaaaaa taaattgatg
      241 aaccccggtc ccgcaatctc gactcgttcg cacacctgag cgagctgaag gttttgcaca
      301 atctcttgag ccacctcgcg tggcgcgcga cgcagggttt tggccaatcc catggccaca
      361 ttggattgat aatctccaaa cttgccttct gtggtcaggc gaatcatcgg atcaacctca
      421 agcaagagtg gtgccacctg actgatggcg caacaaaatt cattcttgat tatttcgaat
      481 actgttttca tgtttctatt cccaatcaag aggaatctcc accacagact ccttatcgtt
      541 caaatccggc atccgcgcaa gatcccccac gatcatcacc ctgaaacgat cagggaaaaa
      601 atacgttccc aagacagcgt taatttcgct caaggtcact tcctcgattt tcttctgata
      661 caacgccaca tagttgggcg ggtaaccgta atgatcatac agcacccgtc ccgtcacaat
      721 tttgaaaggc tgctcaaagt caaacaccag ccgattgaga attctttctt tcgccgcggc
      781 caattctccg tcggtgatat catgccggtt caccatttcg ctcacgatat ttttgatctc
      841 attcaccaca gtcagtgtct tggccgcgtt cgtgcgcgtg ctgatgcgaa aactttcgta
      901 atccgtgccc aaaccaaacg aggacccgag cgagtaaacc agccccaact ccgtgcgcaa
      961 cctcttaccc aatcgcgaac caaaagtcga tccccccaat atctcgttgg ccaaaatgat
     1021 tttgtacttg tccgggttga accgtttttc cccaaaatgc cccatcacga tggcgctttg
     1081 ggaatcatcc ttttgcacca cagcccaccc gggagtccac tctttttcta tcggtttggg
     1141 cggtactgcg tctggcacat tgtgattcca ttcggccaca tggggtttga tcctgctcca
     1201 gaactcatcg gccggatcgg ggctcgtgga cgcgatgatc agctgacggg gggccacatg
     1261 acgggcatga aaatccaaca acattttggt atcaatgggg gcgatggttt gttctgtgta
     1321 ctgccaggca taggggctgt ccgggccata cagaccctgc aaaaattcgc gagaggcgat
     1381 ctccagggaa ctttcgtttc gcctctgaat ggccgttatc ttgttcttct tgatgacctg
     1441 cagtcgttcg ggatcaaacc ggggctggcg caacagatca aaaaaaaggg gcaatgcccg
     1501 ggtgacatct ttttgcagac acttgagcgt caggaaagac agctcctgcc ccgcttgggg
     1561 ggtgatctgg gccgccatca tgtccagctt tgcatccaac tcagaggggg tcaacccctg
//
LOCUS       DATDUN010000009         3064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_249512, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000009 DATDUN010000000
VERSION     DATDUN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249512"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..88
                     /locus_tag="VJC18_00095"
     CDS             <1..88
                     /locus_tag="VJC18_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43805.1"
                     /translation="TPVPGGVGPMTIAMLMKNTLNAAQHLIR"
     gene            205..1284
                     /gene="gcvT"
                     /locus_tag="VJC18_00100"
     CDS             205..1284
                     /gene="gcvT"
                     /locus_tag="VJC18_00100"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HLD43806.1"
                     /translation="MPELLRTPLYDEHKKLGARLVPFAGWEMPVQYSGVIGEHQAVRT
                     RVGMFDVSHMGEFFITGDRAEEFLNRVTTNDVTKLTDGACQYTLLCYENGTVVDDLIV
                     SRVSVNSFIAVVNASNIQKDFDWLVSQNKEGVSLVDKTREFGLIAVQGPKSQSVVNDI
                     LKADFSGLLYYHFRESSFLGHNIFVMRTGYTGEDGFELMVPSAKTPDLWRELLARDVQ
                     PVGLGARDTLRLEVAYSLYGHEISDRIYPLEANLGWIIKLKKGPFVGSAVLNQLKDSG
                     LQRKVVGFEMLDPGIARADCGVFLGDEQVGYVTSGTHSPSLKKSIGLALVKTGWTQIG
                     TEFLVDIRGQKKKAVVVKTPFYVRS"
     gene            1328..1717
                     /gene="gcvH"
                     /locus_tag="VJC18_00105"
     CDS             1328..1717
                     /gene="gcvH"
                     /locus_tag="VJC18_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HLD43807.1"
                     /translation="MGKYPQELKYTKEHEWLKPDAKHYLVGVTDYATEQLGDIVHVEL
                     PKVDDEFDAGDTFGVVESVKSVSDIFMPVAAKVVAVNEELFENPSAINEDPYGDGWLV
                     KLEVSDVSAVNELMSAEAYQAFLAEES"
     gene            1931..>3064
                     /gene="gcvPA"
                     /locus_tag="VJC18_00110"
     CDS             1931..>3064
                     /gene="gcvPA"
                     /locus_tag="VJC18_00110"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HLD43808.1"
                     /translation="MRYLPHTQTDIDEMLKAIGVSEVNELFCSIPEVLRLKDSLNLPR
                     SLSEQELRDEFRELSDSNINLAEGSNFIGAGAYHHYVPSLVSQLVGRGEFLTAYTPYQ
                     AEVSQGTLQAIFEFQTMICELTGMDVANASNYDLSTACAEALMMARRVNKKKKALVAR
                     SVHPHYREVMRTYFKNQDFELVEIPFLKSGRVDVDFIKDHLTDDVAAVLIQSPNFFGV
                     IEDLAPLGTMVEPKPALFVVATSETLSYAVLKSPGEVGADIAIGEGMSLGVGLNYGGP
                     YLGLFATREKFMRSMPGRLVGETTDAKGRRGFVLTLATREQHIRREKATSNICTNQGL
                     CALMCAIYLSVMGNEGLQKLALINIQNRLRLEALIKEKNRGAVL"
BASE COUNT          760 a          697 c          843 g          764 t
ORIGIN      
        1 cacccctgtg ccgggtggcg tggggcccat gacgattgcg atgttgatga aaaacacact
       61 caatgcggca caacatctga ttcgatgaac tgatgtgccg acgtgattcg tgtttacgtg
      121 ttcaagtatt catgtgttta cggacaattt atttcgtgaa cacgttaaca cttgaacaca
      181 tgaacacgat agagagaaaa tgcgatgcca gaattgctac gaacaccatt gtatgatgaa
      241 cacaaaaaac tgggcgcgag actggtgcct ttcgctggtt gggaaatgcc ggtgcaatat
      301 tctggcgtga tcggcgaaca ccaggcggtg cgcacacgtg tgggcatgtt cgatgtgagt
      361 cacatgggtg agtttttcat cacaggtgac agggcggagg agttcctcaa tcgtgtgacc
      421 accaatgatg tgaccaagct gacagatggc gcctgtcagt acacacttct ctgttacgaa
      481 aatgggaccg tggtcgacga cctcatcgtc agccgtgtgt cggtcaattc ttttattgct
      541 gttgtgaacg cgtccaatat tcaaaaagat tttgactggt tggtgtcgca aaacaaagag
      601 ggtgtttcac ttgtggacaa aacgcgggaa ttcggactca ttgcggtgca ggggccaaag
      661 agccagagtg tcgtgaatga tattctcaag gccgatttct cgggtctttt gtattaccat
      721 ttccgtgaaa gtagttttct gggacacaat atctttgtca tgcgaactgg ctatactggc
      781 gaggatggtt ttgaactgat ggtgccctcc gccaaaacac ccgatttgtg gcgagagctt
      841 ttggcacggg atgtccaacc agtgggtttg ggcgccaggg ataccttgcg tttggaagtg
      901 gcttacagcc tttatggtca cgagatcagc gacaggattt atccgctgga ggccaatttg
      961 ggttggatca tcaagctcaa aaaagggccc ttcgtgggat cagcggtttt gaatcaactc
     1021 aaagattccg gtttgcaaag aaaagttgtg ggctttgaaa tgctcgatcc aggaatcgcc
     1081 agggccgatt gcggggtgtt ccttggtgac gaacaggtag ggtatgtgac cagtgggacc
     1141 cattcgccgt cattgaaaaa atccattgga ctggctctgg tcaaaacggg atggacacag
     1201 attggcacag agtttttggt cgacattcgc ggtcaaaaga aaaaggccgt ggtggtaaag
     1261 acaccgtttt atgtgaggtc gtaggctgta actgatgata ttgttaatca atcaacagga
     1321 ggtcaaaatg ggaaagtatc cccaagaact caaatacaca aaggaacacg aatggctcaa
     1381 acccgatgcc aagcattatc ttgttggcgt aaccgattat gccacagaac aattgggtga
     1441 cattgtgcat gtggaactgc caaaagtgga tgatgaattc gacgctggcg atacgtttgg
     1501 cgtggtcgaa tctgtcaaat cggtttccga tatttttatg ccggttgcgg ccaaggttgt
     1561 cgctgtcaac gaagaactct tcgagaatcc ttccgcgatc aacgaagatc cctacggcga
     1621 tggctggctc gtcaagctcg aagtgagcga tgtgtctgcc gtgaacgaac tgatgagtgc
     1681 cgaggcctat caggcgtttt tggcagaaga atcttgatca tacgtgttca cgtgttcatg
     1741 tgttcacgaa gggcaaaagg cccaaggctt aaggcataag gaggattatt tgctgcaact
     1801 acttcaatga gcaacagaca gttggtaatt gatgtagttg ttcgttgaaa caattgaagc
     1861 agttgttttg ccttgagccc tacgccttac gcccctttcg tgaatacgta aacacataga
     1921 tacgagaaaa atgcgctatc ttccacacac acaaacagat atcgatgaga tgctcaaagc
     1981 catcggcgtg agcgaggtga atgagctgtt ttgctccatc cccgaggtat tgcgccttaa
     2041 ggattctttg aacctgcctc gttccctgtc ggagcaggaa ctccgggacg agtttcgtga
     2101 actctccgat agcaacatca atctggccga aggctccaat ttcattggtg ccggagcgta
     2161 tcatcactat gtgccatccc ttgtctcgca attggttggg cgcggtgagt tcttgactgc
     2221 ctacactcct tatcaggctg aggtgtcgca agggaccttg caggccatct ttgaatttca
     2281 gacgatgatc tgtgaactca ccggcatgga cgtggccaat gccagcaatt atgacctctc
     2341 gacggcctgt gccgaggccc tcatgatggc cagacgtgtc aataaaaaga agaaagcgct
     2401 cgtggccaga tcggtgcacc cgcattatcg cgaggtcatg cggacctact tcaagaatca
     2461 ggacttcgag cttgtcgaga ttcctttcct aaaatctggc cgtgtggatg tggacttcat
     2521 taaagatcac ctgaccgatg acgtggctgc cgtcctcatt cagtccccca atttttttgg
     2581 cgtcattgaa gatctggctc ctctcgggac aatggtggaa cccaaaccgg ccctgttcgt
     2641 tgtggcgacc tcagaaaccc tgtcttacgc agtgctcaaa tcaccaggcg aggttggggc
     2701 cgatatcgcg attggtgaag gcatgagtct gggtgtggga ctgaattatg gggggcccta
     2761 tttggggctg tttgcgacca gagaaaaatt catgcgcagt atgcccggac ggctggtggg
     2821 cgaaaccacg gacgcaaagg gcagacgtgg ttttgtgctc acgctggcca cacgtgaaca
     2881 gcacatccgg cgtgagaagg ccacgtccaa catctgtacg aatcaggggt tgtgcgccct
     2941 catgtgcgcg atctatcttt ctgtgatggg gaacgaaggt ctgcagaaat tggccctcat
     3001 caatatccag aacaggctac gactcgaagc tctcatcaaa gagaaaaaca ggggtgccgt
     3061 tttg
//
LOCUS       DATDUN010000010         3828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_374152, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000010 DATDUN010000000
VERSION     DATDUN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374152"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VJC18_00115"
     CDS             <1..277
                     /locus_tag="VJC18_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43809.1"
                     /translation="KHEFVVMRVLDTLERQLPQLALMDFVDLESGQNKTLDLGGNNTW
                     FGDGYRQTTKDLTRFLGNNNIDFLDLRADEDYVEALLKFFMKKDKKR"
     gene            274..834
                     /locus_tag="VJC18_00120"
     CDS             274..834
                     /locus_tag="VJC18_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43810.1"
                     /translation="MTRELPKSIAEMQDIIDIESIVRSAYPGWIKLIIMILLAMFFCA
                     ALIWAIRKLWKRFGGPDSAVVMTIEQLALKDLAKLKKKQWPEQNKWPLYYFELDEILR
                     RYIQLRFGFDLLDKTYEQIRSLLKSGAAAPEFLQLPSVTISELKVFWEGAQLIKFAKM
                     PGTVADCEKDFMMVKIFVETTKQAKS"
     gene            1035..2042
                     /locus_tag="VJC18_00125"
     CDS             1035..2042
                     /locus_tag="VJC18_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLD43811.1"
                     /translation="MNTFFQFHSPWYFLLLIVVVPLFVFDLKKLHLLRFDMSSLSLIR
                     SQRKTLRAIFEFIPPFLRFVALILLVVAVARPQWGNKYTEINSEGIDIILTLDTSESM
                     IALDLNLNGKEATRMDVIKSVVGEFIDGRAYDRMGMVVFGTNAYTQCPLTLDYDILKG
                     YLGLIEIGIAGPATAVGDALLTSVKRLETSQTKSRIVILLTDGTSNAGKISPLRASEM
                     AAEQGIKVYTIAVGRKGRVPFPQKSMFGGWQKAYAVFDVDEDSLRDIAQKTGGQFFKA
                     SDTEALKEVYRTIDQLEKTKVDVKEFSDFEEMYLSFLIPAILLLLGAWVLQRTVFLRI
                     P"
     gene            2115..>3828
                     /locus_tag="VJC18_00130"
     CDS             2115..>3828
                     /locus_tag="VJC18_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLD43812.1"
                     /translation="MQFESHYAIAFFWILPVLLVLFLLLEKKRKRILHGFSPPDVLAK
                     RLPDYAKGTYVVRFLFFVLAIFCLGLAWLKPYSDYEMREVKRKGVDLFFLVDLSQSMN
                     AQDIQPSRITRARYEIVDFLKNLHGDRVGLIGFAGDSFVFVPLTGDYNAFSMFVDELT
                     PEAMPVQGTDIKGAIAKAVKSFQKTSSSRAVVLITDGEDTVGLNQDVLEQIRELNIKL
                     YIMGVGSEEGAPIPLSEGGYLKDRKDQVVLSRLNEPALQQLAKVTGGGYVRSVSGDLD
                     LEQIYQQGIKKSFEDVALNAGQKKLPVYEFQGPILAAIILLFLEILTTNKKRFWRGLW
                     PLRARQKTVPTNKMGILIFLLVCPVLMMVSQSALAMNPFTFEKANHQHEAEAYDKARE
                     TYSDLSKKNPSDAELHYNMGANDYRLNDFTRAEENYTKALDNQDNNMRSKTLFNLGNV
                     RYKQKDMKGALEYYEKALQVNPDYEAAKLNREYVKKVMEGEKNEGTKDENTRDEGEKN
                     EETKDENTKDEGEKNEETKDENTKDEGEKNEGTKDENTKDEGEKNEETKDENTRDEGE
                     KNEGT"
BASE COUNT         1024 a          801 c         1049 g          954 t
ORIGIN      
        1 caaacacgaa tttgttgtga tgcgtgtgtt ggacactctg gagagacaat tgccacaatt
       61 ggcgctcatg gattttgttg atctggaatc cggacaaaat aaaacactgg atttgggggg
      121 caacaacacg tggtttggcg atggttatcg tcaaaccacc aaagacctga cccgattttt
      181 aggcaacaac aacatcgatt ttttggacct gagggccgat gaggattacg tggaggccct
      241 gttgaaattt tttatgaaga aggacaagaa gagatgaccc gtgaactgcc aaaatccatt
      301 gccgagatgc aggacatcat cgatatcgag agcatcgtga gatctgccta tcccggctgg
      361 atcaaactca tcatcatgat cctgctcgcg atgttttttt gcgcggcgct gatctgggcg
      421 atcaggaaac tctggaagcg ctttggcgga ccagactctg ccgtcgtcat gactatcgag
      481 caattggcat tgaaagatct ggccaaactc aaaaagaagc aatggcccga gcaaaacaaa
      541 tggcctcttt attattttga actcgacgaa attctgcgcc gctacattca acttcgtttt
      601 gggtttgatc tcttggacaa aacttatgag cagatccgat cgttattaaa gagcggggcg
      661 gcggcgcccg aatttttgca gttgccttcg gtgacaatca gtgagctcaa ggtgttttgg
      721 gaaggggctc agctcatcaa gttcgccaaa atgccgggca cggtggcaga ttgtgaaaaa
      781 gattttatga tggtgaaaat atttgtagaa acgacgaaac aagcaaaaag ctgaaaggca
      841 tatgaatatt gaatatagaa caaggaatgt cgaatgtcga aatttgtcat ccctgcgcac
      901 gcagggatcc agaattatta taaacatgga ttccagcatt cgctggaatg acaatcattt
      961 cgaaattcct tgttccttgt tcgtgattcg atattcgttt tcttcccttt gaactttcag
     1021 ctttgatctg tattttgaat acttttttcc aattccattc cccctggtat tttttgttgt
     1081 tgatcgttgt ggtgccgctg tttgtgtttg acctcaaaaa actgcacctg ctccggttcg
     1141 acatgagttc cctctcgctc atccgatcac agcgaaagac gctcagggct atttttgagt
     1201 ttataccgcc gtttttgaga tttgttgcgt tgatcctcct ggtggtggcg gtggcccggc
     1261 cgcagtgggg taacaaatac actgagatca atagtgaggg gatcgatatc atcctcacac
     1321 tggacacctc ggagagcatg attgcgcttg atctcaacct gaacggcaaa gaggccacac
     1381 gtatggacgt gatcaaatca gtggtgggtg agtttatcga tggacgcgct tacgatcgta
     1441 tggggatggt tgtttttggg accaatgcgt acacgcagtg tcctctcaca ctggattacg
     1501 atatcctcaa gggttatctg gggctcattg agataggcat tgccggtccc gcaacggcgg
     1561 taggggatgc cctgctcacg tcggtgaagc gtttggaaac atcccagacc aaatcgcgta
     1621 ttgttatttt attgaccgat gggaccagca atgctggcaa gatatcccct ctccgggcat
     1681 ccgaaatggc ggccgagcag gggatcaagg tgtacacgat cgccgtggga cgtaagggca
     1741 gagtgccttt cccccagaag agtatgtttg gcggctggca gaaggcctat gctgtgttcg
     1801 atgtcgatga agacagcctg cgcgacatcg cccagaaaac gggaggccaa tttttcaagg
     1861 ccagcgatac cgaggcgctc aaagaggtgt atcgtacgat tgaccagttg gaaaaaacca
     1921 aggtcgacgt gaaagaattt tcagattttg aggagatgta tttgtctttc ttgatcccgg
     1981 caatcctgtt gctgttggga gcctgggtgt tgcagaggac agttttttta cggataccgt
     2041 gaaatcacgt gttcacgtga aaatgcattt tcgtgaacac ttgaatactt gaacacgtaa
     2101 acacgaagtt tttgatgcaa ttcgaatcac actatgccat cgcattcttc tggattttgc
     2161 ccgttttgct ggtcctgttt ttgcttttgg aaaaaaaacg caaacggatc ttgcacggct
     2221 tttccccccc cgatgttttg gccaagcgtt tgcctgacta cgcgaaaggg acttatgtcg
     2281 tgaggttttt attttttgtg ttggctattt tttgtctggg gttggcctgg ctcaagccct
     2341 attccgatta tgaaatgcgc gaagtcaaac gcaaaggtgt cgacctgttt tttttggtgg
     2401 atctgtcaca atccatgaac gcgcaagaca tccaacccag tcgcatcacc cgcgcgcgat
     2461 acgaaattgt tgattttctc aaaaatctgc atggtgatcg tgtggggctc attggttttg
     2521 cgggtgattc gtttgttttt gttcccctga cgggcgatta caatgcgttt tcgatgtttg
     2581 tggatgaact cactcccgag gccatgccgg tgcagggcac ggatatcaaa ggggctatcg
     2641 cgaaggccgt caaatctttt cagaaaacaa gctcgtcccg agccgtggtc ctcatcactg
     2701 atggggaaga cacggtcggt ttgaatcaag atgttttgga gcagatcagg gagctcaaca
     2761 tcaagttgta tatcatgggc gttggcagtg aggaaggggc cccgattccc ctgagcgagg
     2821 gtgggtatct caaggatcga aaggatcaag tggttttgtc gcggctgaat gaacctgctc
     2881 tgcaacaact ggccaaggtt actggcgggg ggtatgtcag atcggtgagc ggtgatctgg
     2941 atctggaaca gatctatcaa cagggcatca aaaaatcttt tgaagatgtc gccctgaacg
     3001 cggggcagaa gaaacttccc gtgtacgagt tccaggggcc gattctggcg gcgatcatct
     3061 tgctgttttt ggagattctc acaacaaaca aaaaaagatt ctggcggggt ctgtggcctt
     3121 tgcgggcaag gcaaaaaact gtgccgacca acaaaatggg gattctgatt ttcttgctgg
     3181 tctgtcctgt cttgatgatg gtgtcccaat cagctctggc gatgaacccc ttcacgttcg
     3241 aaaaggccaa tcaccaacat gaggccgagg cgtacgataa ggcgagggaa acttattctg
     3301 atttgtcgaa aaaaaatccc agtgatgccg aattgcatta caatatgggg gccaatgact
     3361 atcgtttaaa tgactttaca cgtgctgagg aaaattacac caaagcttta gataatcagg
     3421 ataacaatat gagaagtaaa acccttttta atttaggaaa tgttcgatac aagcaaaaag
     3481 atatgaaagg agccctggag tattacgaaa aggccctgca ggtgaatccg gattacgagg
     3541 ccgcaaagct caacagggag tacgtgaaga aggtcatgga gggggagaag aacgaaggga
     3601 cgaaggacga aaacacgagg gacgaggggg agaagaacga agagacgaag gacgaaaaca
     3661 cgaaggacga gggggagaag aacgaagaga cgaaggacga aaacacgaag gacgaggggg
     3721 agaagaacga agggacgaag gacgaaaaca cgaaggacga gggggagaag aacgaagaga
     3781 cgaaggacga aaacacgagg gacgaggggg agaagaacga agggacga
//
LOCUS       DATDUN010000011         4144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_581920, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000011 DATDUN010000000
VERSION     DATDUN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581920"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2073)
                     /locus_tag="VJC18_00135"
     CDS             complement(<1..2073)
                     /locus_tag="VJC18_00135"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HLD43813.1"
                     /translation="MKNQQTIRWVLWILSVTAILLTAQVAKAATLNVDTNDPACSDIT
                     GTPYCTIQVAVNNTASSDVIEIAAGVYAENVTVADKSLTFIGENKDTTIIDGGHANSV
                     LTVTSVTNLNINLEQLTLRNGTAAVGGGINMALGNLVLSDTDVSNNTVTHVGGIYVGS
                     GSIELVDSSVVDNTATGTCGGVYIASGDLTLINSHIDDNTAAGGGGLYVINGALTLTE
                     SSVDDNTSSNIGGLYHAGGFLTMDHASVSNNQTTTSYGGMYHFSGDVNITDSTIDGNT
                     AAGNMAGAYIESGTVTITNTSISQNNSVGTVGGYFQNTGNLTITGSTLDENTSQTGTV
                     GALHHANGITTITDSTIDGNSATGIGAIYQQTGTLSITNSSVRDNQSSSTAGAIMSVG
                     DLNVLNSTIENNHADGTAGAIYQTTGTFRLTNSLIANNTSDQHIGGMYVTGNFIGTNS
                     TISGNTADDHIGGLYLISGGQLYNMTIVNNSADMDNDGIGDGGGVYAGAGVVTVSNSI
                     IALNEDRGGESPDCAGNVTSGDYNLLGQDTGCTFTALANDQIGTGADPIDPEISNLFD
                     NGGETFTHSLLDVSPAIDAGNPAGCFGDGGVTLTTDQRGYPRPSGSACDVGAYEVGDC
                     GDGHLDYDEECDDGNGVNTDACTNMCLTATCGDGIVQTTVDECDDGNTVDGDGCDSAC
                     NEEAAVTPT"
     gene            complement(2225..3037)
                     /locus_tag="VJC18_00140"
     CDS             complement(2225..3037)
                     /locus_tag="VJC18_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLD43814.1"
                     /translation="MPISFIKRKIRRVSRRVRNWSTIASLTLKGNRVGKNTRLANAKH
                     PILMIYGFGSSRRTMAILETRLRQDGHVTFSLNLGGFIGTFNTVSIEESARYIDKKVE
                     EFYKKYNIQGKLNLVGHSKGGLIGSYWIKFLNGARRVNTLVTLGAPYNGTPWGYFSYI
                     PGVSLVLKSLKQLKPNSIFLQRLRETPYPKRVKVFSVYSKDDNACPFPASVLEEADNV
                     KNIEIPSATHSELLVKKTVFNIIRHALKNEMPESLIDATRKNIVEFFGTREI"
     gene            3176..3880
                     /gene="lipB"
                     /locus_tag="VJC18_00145"
     CDS             3176..3880
                     /gene="lipB"
                     /locus_tag="VJC18_00145"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HLD43815.1"
                     /translation="MYEKHFPSEIIINSLGRIPFAEAVALMEETHRKRCANEVADTLF
                     LLEHEPVITKGRRLKGQNIVVHPEIVKRGIPVCDADRGGLLTYHGPGQIVVYFVIKVA
                     ERFLGVQQMVLALERILLEFLRCQRRGRSRPASTAPFLNGYAVLNVHTREGHPGIWVG
                     EKKIASIGLRIKNGVSLHGISLNICNDLSVYDYFDPCGMAGKVMTNLEQVWGRGIEKQ
                     EFEHLSNALAKRLCHM"
BASE COUNT          964 a          993 c         1094 g         1093 t
ORIGIN      
        1 cgtcggggtg actgcggctt cttcgttgca ggcgctgtca caaccgtcac catcaactgt
       61 gttgccatca tcacattcat ccacagtggt ctgaacgatg ccatcaccgc aggtagctgt
      121 gagacacata ttggtgcagg cgtcggtatt cacaccatta ccatcgtcgc attcttcgtc
      181 ataatccaag tgaccatcac cgcagtcgcc aacttcgtag gcgcccacat cacaggcaga
      241 gccagagggc cgaggatagc cgcgttgatc tgtagtcagt gtgacgccac catcaccaaa
      301 acaacctgca ggatttccgg cgtcaattgc cgggctaaca tccaacaagg agtgagtgaa
      361 cgtctcgccg ccgttatcaa acaaattact gatttcggga tcgatgggat ctgcccctgt
      421 tccaatttgg tcgttggcta acgctgtaaa cgtacagcca gtgtcctggc cgagcagatt
      481 gtaatcacca gacgtcacgt tgccagcaca gtcaggtgat tcaccgcccc gatcttcgtt
      541 aagagcaatg atcgaattgc ttacggtaac cacacccgct ccagcataca caccgccgcc
      601 gtcaccaatc ccatcattgt ccatatcagc tgaattgtta acaatcgtca tgttgtagag
      661 ttgtcctcct gaaatgaggt acaacccacc gatgtggtcg tctgccgtat tgccgctgat
      721 cgtggagttg gtgccaataa aattcccggt gacgtacatg ccacctatgt gttgatcgga
      781 agtgttgtta gcaatgaggc tgtttgtgag tctgaatgtt cctgtcgttt gataaatggc
      841 accagctgtt ccgtcggcgt ggttgttttc aatagtgctg ttcaaaacgt tcaaatcccc
      901 gaccgacata atggcgccgg cagtgctgct ggattgattg tcacgcacac ttgaattggt
      961 gatcgataag gtgccggtct gttggtagat ggctccaatg ccagtggccg agttgccatc
     1021 gatggtggaa tcagtgatgg ttgtgatccc gtttgcgtga tggagggcac ccacagtccc
     1081 tgtttgagag gtgttttcgt ccagggttga acctgtgatc gtcaaattgc ctgtattctg
     1141 aaaatacccg ccgacagttc caacgctgtt attctgactg atgctggtgt ttgtgatcgt
     1201 gacggtgccg ctttcgatgt aagccccggc catgttgccg gcggccgtat ttccgtcaat
     1261 ggtgctgtca gtgatgttca cgtcaccgga aaaatgatac atcccaccat agctggttgt
     1321 ggtttggttg ttggaaacag aggcgtggtc cattgtgagg aatccaccgg cgtgataaag
     1381 tccgccaata ttgctggagg tattatcatc aaccgaggat tcggtgagtg tgagggcccc
     1441 gttaataaca tacaacccac cgccaccagc cgccgtatta tcatcaatgt gggaattgat
     1501 cagcgtcaaa tcacccgaag caatgtagac gccgccacag gtgcctgttg ccgtgttgtc
     1561 cacgacagag ctgtcgacta gttcaatgga tccgctcccc acataaattc cgccaacatg
     1621 ggtcacggtg ttattgctga cgtcggtgtc ggagagcacc aggttgccca gggccatgtt
     1681 gatcccgcca ccaactgcgg cagttccgtt acgcagggtc agctgttcca gattgatgtt
     1741 cagattggtg acgctcgtga cagtcaaaac gctgttcgcg tgtccgccgt caatgatggt
     1801 cgtgtccttg ttctcaccaa tgaaggtgag actcttgtca gcgaccgtaa cattttcggc
     1861 gtagacgcca gcggcgatct cgatcacgtc agaactggcc gtgttgttta ccgccacttg
     1921 aatcgtgcag tagggtgtgc ccgtgatgtc gctacacgct ggatcgtttg tgtccacgtt
     1981 cagtgtcgcc gccttggcca cctgcgctgt gagcaagatc gcggtgactg agaggatcca
     2041 caggacccat cttatggttt gttgattttt cataaaaact ccttggttta aaaagtgatt
     2101 gaacatggtg cttcaataac aaacttaaac gggagctgat acgaaaatac cgataacctg
     2161 ttccctgaag gatacagagg aaaaacatca cgtagcaggg acaggaattg gcctggcagg
     2221 gagatcagat ttctctggtg ccaaaaaatt ccacgatatt tttgcgggtc gcgtcgatga
     2281 gcgactccgg catttcgttt ttaagagcgt ggcggatgat gttgaacact gtttttttga
     2341 ccagcagttc cgagtgagtg gcactgggga tctcgatgtt tttgacattg tcggcctctt
     2401 caagcacaga ggcagggaaa gggcaggcgt tgtcgtcctt ggaatagact gagaaaacct
     2461 tgacgcgttt ggggtagggg gtttcgcgta aacgctgtaa aaaaatgctg ttgggtttga
     2521 gttgtttgag actcttgagc accagactca cgcccgggat gtacgaaaaa taaccccacg
     2581 gggtaccgtt gtaaggtgcg cccagcgtga ccagcgtgtt aactcttctg gcgccattca
     2641 ggaatttgat ccagtaggat ccaatgagcc cccccttcga atgaccaacc agattcaatt
     2701 tgccctgaat gttatatttc ttataaaatt cctcaacttt tttatcgatg taccgggctg
     2761 attcttcgat cgagacggtg ttgaacgtcc cgatgaaccc tcccagattc aaagagaatg
     2821 tgacgtgacc atcctgtctg aggcgcgttt ccagaatggc catggttcgg cgcgaggaac
     2881 caaatccgta aatcatgagg atggggtgtt tggcattggc caggcgcgtg ttcttgccaa
     2941 ccctgttgcc ttttagcgtc agcgaggcta tcgtggacca gttacgaacg cggcgcgaca
     3001 ccctgcgaat tttgcgtttg atgaacgata ttggcattgg gaacattcta ctcgattatg
     3061 tgtattatgg caaatatcgg cttgagcttt gtcatatggt gtttaggtgt ttacgtgttc
     3121 acgtattcac gaggggagta gtccttaagt ttgtcccagg cttgaagaac aggttatgta
     3181 cgaaaagcat ttcccatctg agatcatcat caactccctg ggtcgtatcc cttttgccga
     3241 ggctgttgcc ctcatggaag agacgcatcg taaaagatgt gccaacgaag ttgcggatac
     3301 tttatttctt ttggagcacg agcccgtcat cacaaagggg agaaggctca agggacaaaa
     3361 tattgtggtt caccccgaaa tagtcaaacg tggcattcct gtttgtgacg cggaccgtgg
     3421 cgggttgctt acgtatcatg ggcctgggca aatcgtggtg tattttgtca tcaaagtcgc
     3481 agaacgattt ttgggtgttc aacaaatggt gttagcgctc gaaagaattc tattggaatt
     3541 tttgcgctgt caacgtaggg gccggtcgcg accggcctct acagcaccat ttttaaacgg
     3601 gtatgcagtt ttgaatgtcc acacgcgcga gggccatccc ggcatttggg tgggcgaaaa
     3661 aaagatcgcc tccattggat tacgtatcaa aaatggtgtg agcctgcatg ggatcagcct
     3721 gaacatctgt aatgatctga gcgtgtacga ttattttgat ccctgcggca tggcgggcaa
     3781 ggtgatgacg aatttggaac aggtgtgggg cagggggatt gaaaaacaag agtttgaaca
     3841 cctatcgaat gctttggcaa aacggttatg tcacatgtga acatcactga tgaaacgagc
     3901 tcaaagctga aagctcaaag gaaaagcgtg ttggggaaag tgtgccgcat gtcactataa
     3961 caattgctac agttgcaatg attgcccatt gctaattgct ataattgtct tctttcctca
     4021 cccgtgcttg tcacccccaa tatcttgaac agctccttgc ccttgccggg tagttcgatc
     4081 tccacaacat cattttccat tttgccccag atggcttggc ccacgtcggt gtaattggaa
     4141 atgt
//
LOCUS       DATDUN010000012         1566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_374323, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000012 DATDUN010000000
VERSION     DATDUN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374323"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..>1566
                     /locus_tag="VJC18_00150"
     CDS             157..>1566
                     /locus_tag="VJC18_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HLD43816.1"
                     /translation="MSKIVLDLARIIVETALLPYQSWMNLDALLRSLYRRFVSHRHLL
                     EWSTAQKTQDNAQTKAPKYLTIIASSGGISTFLMLLFLLFKPDCLLLVFPWLVLWLAS
                     PLMAWSLNRRPHVRTPVSFLKEDERRFLRKISRRTWRYFADFVNEKSSWLPPDNYQVS
                     HQNQLAMRTSPTNIGLWLASVLSARQFGYLTGDDVTHTLLKTFGTLNQLERYEGHLLN
                     WYDIQTLTPLNPRYVSTVDSGNFLGALWTLQHGLLELMHRPILDSLAFEGLCDTCEIL
                     KESAQQETYVGFDTQDIDDLLKEWNKPHAHVGEALAVLWKTKITVRNLAQKARGINTT
                     TGNVTYWADQLENLIQSWLSVSDRYLNWIEILNEKNVAGIPALLQAPSLFDLARGQVP
                     AIIALENMRHQTATEEVSLIDWFDRILTAFSKSKWLAGEALSVTEQLVHECDNFAEAI
                     NMQFLFNQERKLFSIGYNVS"
BASE COUNT          363 a          391 c          385 g          427 t
ORIGIN      
        1 ccaactgcta gtttggcttt gctgatggcc gcttgtctta cctcttttcg agccggcgtt
       61 atcgccagtg ggctggtgat ctttcaattc atgttccatt cgttggccca gcatttctct
      121 tgggcctcga cgcgtcaggg tttcaaaagc ctttcattgt caaagatagt tttggatctg
      181 gcgcgcatca tcgtggaaac cgcgctcctg ccgtatcaat cctggatgaa tttggatgcc
      241 ctgttgcgca gtctttatcg acgtttcgtt tcgcaccgtc atcttctgga atggagcacg
      301 gcacaaaaga cgcaggacaa tgcacaaacc aaggcaccaa aatatttgac catcatcgct
      361 tcttccggag ggatcagcac gttcctgatg ctgctgtttc tgttgttcaa gcccgattgt
      421 ctgctcttgg tttttccgtg gttggtactt tggctggcgt ctcctttgat ggcttggtct
      481 ctcaatcggc gtccccacgt cagaacaccc gtgtcatttc ttaaagaaga tgaacggcga
      541 tttttaagaa aaatatcgcg acgcacctgg cgctactttg cggattttgt gaacgagaaa
      601 tcctcctggt tgccacccga taactaccag gtctcccatc aaaaccaact cgccatgcgg
      661 accagcccca ccaacatagg tctgtggttg gccagcgttt tgagcgcgcg tcagttcgga
      721 tatctgactg gggatgacgt gacgcacacc ctgttaaaaa cttttgggac tctgaatcaa
      781 ttggagcgtt atgaggggca tctcctcaat tggtacgaca ttcaaacgtt aacgccttta
      841 aacccccgct atgtttccac agttgatagc ggcaattttt tgggagcact ttggaccttg
      901 caacacgggc ttttggaact catgcacagg ccgatattgg acagcctggc ctttgaaggg
      961 ctctgcgata cgtgtgaaat cttaaaagag tctgcgcaac aggaaaccta tgttggcttt
     1021 gatacgcagg atattgatga tctgttgaaa gaatggaaca agcctcacgc tcacgttggg
     1081 gaggctcttg cagtcctttg gaaaacaaaa atcacagtga gaaatctggc gcagaaggcc
     1141 cgcggcatca acaccaccac aggaaacgtg acctactggg ccgatcagct cgaaaatctc
     1201 atccaatcct ggttgagcgt ttctgaccgt tacctgaact ggattgagat cctgaacgaa
     1261 aaaaatgtgg cggggattcc cgctcttttg caagccccct cgctttttga tctggcccgg
     1321 gggcaggtgc cagccatcat tgcgcttgaa aacatgcgtc accagactgc gaccgaagag
     1381 gtttctctga ttgactggtt tgatcgcatt ttgacagcct tcagcaaatc aaaatggctg
     1441 gcgggtgaag ccttaagtgt gactgaacag cttgtgcatg agtgtgacaa ttttgcagaa
     1501 gccatcaaca tgcagttttt attcaaccag gagcgcaaat tattttcgat tggttacaat
     1561 gtttct
//
LOCUS       DATDUN010000013         1843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_333037, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000013 DATDUN010000000
VERSION     DATDUN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333037"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VJC18_00155"
     CDS             complement(<1..1072)
                     /locus_tag="VJC18_00155"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HLD43817.1"
                     /translation="MKTILSYLFTLLLLATFVACSSNSVRETATLQSQSIMSVPVKLA
                     TLDETKLSATLNVYDDAAKLITAKDLTIANGQVSSESFELTKNQTYVFVIVFYYTLNS
                     EQIPVAYVLLSQELTGNSLQIAFDSADIVTDPASASADTSLDITSYGIPNLDSDGDGV
                     GNLAEIGSGEICEQDYPLYRDADGDGYGASSQVVYNCTATKEGYVANGTDCDDASSAI
                     HPGATEICDGIDNDCGGTIDEDGLTRYYYDGDDDGYGVTGEYVDACALNGNYTTAERG
                     DCNDANSAIYPNAFEGCSDDGIDNDCDSVYVEGFLCKDYIYDDPLGEDDVVGWSKIIR
                     GDSASDNLGEWVSSAGDVTGDGV"
     gene            complement(1114..1500)
                     /locus_tag="VJC18_00160"
     CDS             complement(1114..1500)
                     /locus_tag="VJC18_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLD43818.1"
                     /translation="MNRESWGKAMKIQSYKDLEVWKKGIEIVKTVYELTRRFPKCELF
                     GLISQMQRAAVSIPANVAEGYARQYSKEFSRFCSISLGSCSELETLVIIAKEQRYLTE
                     NDFIRISEFLDHESRMLMSLTKKLAH"
     gene            complement(1518..1700)
                     /locus_tag="VJC18_00165"
     CDS             complement(1518..1700)
                     /locus_tag="VJC18_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLD43819.1"
                     /translation="MFIYFGISPGVFMTAPVTLGTSSAPGEVATFDFDNDGDEDIVVS
                     DYASGHLMFYTNQMVE"
BASE COUNT          500 a          499 c          361 g          483 t
ORIGIN      
        1 gcacaccatc acccgttacg tctccggctg aggaaaccca ttcgcccaga ttgtcgctgg
       61 ccgaatcgcc cctaatgatt ttagaccacc caacaacatc atcttcgcca agaggatcat
      121 catagatata atctttacac aaaaacccct ccacgtaaac actgtcgcag tcattatcga
      181 ttccatcatc tgaacaccct tcaaaagcat tgggataaat tgcgctgttg gcatcgttgc
      241 aatcacccct ctcggcagtc gtgtaattgc cattcagtgc acacgcgtca acatactccc
      301 cagtcacacc atacccatcg tcatcaccat cataataata gcgtgttagc ccatcttcgt
      361 cgatagtccc gccacaatca ttatcgatac catcacaaat ttcggtcgct ccgggatgaa
      421 tcgcagaaga tgcgtcatcg caatcggtgc cgttggccac ataaccctct ttggttgcag
      481 tacaattata aacaacctga ctactggccc catacccgtc accatccgca tcgcgataca
      541 agggataatc ttgttcacat atttctccac tcccaatctc tgccaaattc cccaccccat
      601 ccccatcact atccagattg gggatgccgt aggacgtgat gtcgagcgaa gtgtcggcac
      661 tggcagaggc agggtcggtc acaatgtcgg cagaatcaaa cgcgatttga agggaattgc
      721 ccgtaagctc ctgcgacagc agaacatacg ccacggggat ttgctcactg ttcagtgtgt
      781 aataaaacac aatcacaaaa acataggtct ggttttttgt gagttcaaaa ctctctgacg
      841 acacctgacc attggcaatg gtgaggtcct tggccgtgat gagtttggcc gcatcatcgt
      901 acacattgag tgtggcggag agttttgttt cgtcaagcgt tgccagtttg acgggaacgg
      961 acatgatgga ttgcgactgc aatgtcgctg tttcacgaac agagttggat gagcaagcga
     1021 caaatgttgc cagcaacaag agcgtgaata gataagaaag gatggttttc ataatgacct
     1081 cgtggatcgt gaatcgtgga tcgtggatcg atgtcaatgg gcaagttttt tagtcaggct
     1141 cattaacatt cgactttcgt gatccaaaaa ttctgatatt ctaataaaat cattctccgt
     1201 taaatagcgt tgttcttttg caataatcac aagtgtttcc agttcggaac aagaccccaa
     1261 tgaaatactg caaaatcgac tgaactcctt cgaatattgc cttgcatatc cctctgcaac
     1321 atttgcaggt attgatacag ccgctctttg catttgcgat atcaaaccaa aaagttcaca
     1381 ctttggaaaa cgtcttgtta attcatatac agtttttaca atttctatcc ccttcttcca
     1441 aacctccaaa tctttgtacg attgaatctt catcgccttt ccccacgatt cacgattcac
     1501 gattcacgat ccacgattta ttccaccatc tgattcgtat aaaacatcaa atggcccgag
     1561 gcataatcgc tcaccacgat gtcctcatca ccgtcattat caaagtcaaa cgtggcgact
     1621 tctcccggtg cggagctggt ccccagcgtg accggcgcgg tcatgaatac acccggcgag
     1681 atgccaaagt aaataaatat acggccctcg tttctcgcct tactcctcca gcgtaatcaa
     1741 atacacggcg ccagcgttca aaaaactatc gttgtcttca gcattgctac ctatcagaat
     1801 ttcttcatgg ccatcctgat tgacatcacc agcataggcc acc
//
LOCUS       DATDUN010000014         1582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_582340, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000014 DATDUN010000000
VERSION     DATDUN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582340"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..>1582
                     /locus_tag="VJC18_00170"
     CDS             36..>1582
                     /locus_tag="VJC18_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43820.1"
                     /translation="MDVRMLMAETLDHQAIEQAIVHYGGKEVSLFSANEPARGSLTVQ
                     QDVVGYYHQASGGDLQILRVLLHAHLLNGTLSVNAERVLIFGRARPQQLTNREIEALH
                     LSALDHLSPLERSVFETISVLVGDDVIFYDQELPQHQAGELGPVALARILASLKEGGY
                     ITPEGHDGYRIIKVMLRDMVQRQLAHSQQTKARHQQIVDHLSAFERKSARDWALYGYH
                     LRRVGDYQQAFLSYEKAAQGCQKPGSMSLSYAYFVEMEGLLPHFDGADRLERLAGLLA
                     QRLAVRREMLQVDQGSQEWRDDLQTYASLRDDPAFKALPQARQDEVILNGLAIFPGEY
                     WLKQRPNEVAAFYEHVEREQAAFIHSLAPALRILFHIRAMGACQMINSRGQQEAADAH
                     VQKAIALIVGDERATIRDHERVFAATVLTQNPVLFARVCLNIASRYSVHGPQYAVAEP
                     YALMAIELYERMGHHVMTCYARMALGINLNNRGLFARAEEVLQVNYVQGAREPYVRNY
                     ALMHLAVS"
BASE COUNT          346 a          372 c          473 g          391 t
ORIGIN      
        1 aattgaaaca tcagtatttt attggggagt ctcatgtgga tgtgcgcatg ctgatggctg
       61 agaccttgga tcatcaagcg atcgaacagg ccatagtaca ctatggtggg aaagaggttt
      121 ctctgttttc cgcaaatgag cctgctcgcg gttctttgac tgtgcaacag gacgtggtgg
      181 gttattacca tcaggcctcg ggaggtgacc tgcagatact gagagtactc ctgcacgcgc
      241 atttgttgaa cgggaccttg tctgtcaacg cagagcgtgt cctgattttt ggtcgggcga
      301 gaccacaaca actgacgaac cgtgaaatcg aggcccttca tctatcggcg ctggaccatc
      361 tttcaccctt ggagaggagc gttttcgaaa ccatctcggt gctggtgggt gacgatgtca
      421 tcttttatga tcaagaattg ccccagcatc aggcgggtga gttgggtccc gtggccttgg
      481 ccaggatctt ggcatcgcta aaagagggtg gctacattac cccggaagga cacgatggtt
      541 accggatcat caaggtcatg ttacgtgata tggtccaaag acagcttgcc catagccaac
      601 aaacgaaagc gaggcatcag cagattgtcg atcacctgag cgcgtttgag cgcaagagtg
      661 cccgtgattg ggccctttac ggctatcatt tgcgtcgtgt gggggactat cagcaggcct
      721 ttctgagtta tgaaaaagcg gcgcaaggat gccaaaaacc gggttccatg tctctctcct
      781 acgcgtattt tgtggagatg gagggcttat taccgcattt tgacggggcg gacagacttg
      841 agcggttggc cggtttattg gcacagcgtt tggctgtgcg tcgggagatg ttgcaggtcg
      901 atcaaggttc gcaggaatgg agggatgatc tgcagacgta tgcctctttg agagacgacc
      961 cggcctttaa ggccttgccc caggccagac aggacgaagt gattctgaac gggttggcca
     1021 ttttcccggg agaatattgg ctcaaacaac gccccaacga ggtggccgcg ttctatgaac
     1081 atgtcgaacg ggaacaggcg gcatttattc attctctggc ccctgccttg aggatcctgt
     1141 ttcatatccg tgccatgggg gcctgccaga tgatcaattc acgtggacag caggaggcgg
     1201 ctgacgcgca tgtgcaaaag gccattgccc tgattgtggg tgacgagaga gcgaccattc
     1261 gtgatcacga acgtgtcttt gctgctacgg tgttgacgca gaatcctgtt ttgttcgctc
     1321 gtgtttgtct gaacatcgcc tcacgttatt ccgtccatgg gccacaatac gcggtggctg
     1381 aaccgtacgc cctgatggcc atcgaactgt acgagcggat gggtcatcat gtgatgacct
     1441 gctatgcgcg tatggccttg ggaatcaatt tgaacaaccg ggggttgttt gcgcgggcag
     1501 aagaggtgtt gcaagtgaat tatgttcagg gggcaaggga gccctatgtc agaaactacg
     1561 cgctcatgca cttggcagta tc
//
LOCUS       DATDUN010000015         2487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_250135, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000015 DATDUN010000000
VERSION     DATDUN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250135"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="glmS"
                     /locus_tag="VJC18_00175"
     CDS             <1..966
                     /gene="glmS"
                     /locus_tag="VJC18_00175"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HLD43821.1"
                     /translation="VRKDEFKIQIVACGTSWHAGLTARYWIENLAKVPVSVELSSEFR
                     YRQPLVNENTLVVAISQSGETADTLAAIRESKKMGAKILSICNVIGSSIPRESDVTIY
                     THAGPEIGVASTKAFTTQIAVLYMMAVKLALQKGLLNEDETRVRIKALVELPNLLKTF
                     LTDIKGLQEMTECLHNDEHCYFIGRGIQYPIILEGALKLKEISYVHADGYAGGEMKHG
                     PIALIEEGVPLVAVVLKDDLYEKMVSNVQEMKSRGAFVLGIISKGDDDMKQICDICIE
                     VPETHPDLMPFVSVIPLQLLAYYVADAKGTDVDQPRNLAKSVTVE"
     gene            1092..>2487
                     /locus_tag="VJC18_00180"
     CDS             1092..>2487
                     /locus_tag="VJC18_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLD43822.1"
                     /translation="MQLNPKQQEAVEHRDGPLLILAGAGSGKTRVLVERIVRLMQTGG
                     VNPWNIMAVTFTNKAAKEMKERVAQSMGQRANDLWMATFHSFCLRLLRRHAEVLNYHT
                     HFTIYDDLDQRSLIKKILKDMQLSDTVYKPASIQYHINKAKNDGIKAEDFPSGGDFFL
                     KKVGAIFAEYQKHLILNQAMDFGDLIVNALHLFGKSPEILSKYQNQFQHILVDEYQDT
                     NKSQYALIRLLADKWKNVCVVGDDDQSIYKFRGAEIRNILDFQRDYPQAKVIRLEQNY
                     RSTQNILNASNAVIRGNTERMGKELWTQNGQGECIRMFGGQTDHDEAEFVCSSIMSHR
                     EKYRLGDMAIFYRTNAQSRILEDSLRKHKIPYRIFGGIKFYDRAEIKDMMSYLKTLVN
                     PSNTISLKRILNTPARGIGKTTVERIDAAADRESACFWDVISSVSDPRWALELNTGTM
                     TKIEDFVKLIARLNQ"
BASE COUNT          683 a          628 c          615 g          561 t
ORIGIN      
        1 gtcaggaaag acgaattcaa gattcagatc gttgcctgtg gcaccagttg gcacgcggga
       61 ctcacagcga gatactggat tgaaaatctc gcgaaggtac ccgtgagcgt cgagctttcc
      121 agtgaatttc gttatcgcca gccactggtc aacgaaaaca ccttggtcgt agccatcagt
      181 caatcaggcg aaacagcaga caccttggcc gccatacgcg aaagcaaaaa aatgggggcc
      241 aagatattgt ccatctgcaa cgtaatcgga tccagcatcc ctcgtgaatc cgatgtgacc
      301 atctacacgc acgcgggacc agaaatcggt gtggccagca ccaaggcctt caccacgcaa
      361 attgccgttc tctacatgat ggccgtcaaa ttggccttgc aaaaaggact gctcaacgaa
      421 gacgaaaccc gagtgcgcat taaggcactt gtggagctac ccaatttgct caaaactttt
      481 ctgactgaca tcaagggtct tcaggaaatg acagaatgtc ttcacaatga cgaacattgt
      541 tattttatcg gtaggggcat tcagtacccc attattctgg aaggggcgct caaacttaag
      601 gagatcagtt acgttcacgc agacggctat gctgggggcg aaatgaagca cggtcccatt
      661 gccctcatcg aagaaggcgt tcctctggtt gctgtcgttt tgaaggacga tttgtacgaa
      721 aaaatggttt cgaacgttca agagatgaaa tctcgtgggg cctttgtgct gggaatcatc
      781 tcgaagggtg atgacgacat gaagcagatt tgtgatatct gcatcgaagt ccctgagact
      841 catcccgatc ttatgccctt tgtgtccgtg ataccattgc aattgctggc atactacgtc
      901 gccgatgcca agggcaccga tgtcgatcaa ccccgtaatt tggcaaaatc tgtgacggtc
      961 gaataacgtt agcgcgtgga tcgtgaattg tgaatcgtga atcgaaaaat tcaatatttg
     1021 accttcgatc cacgaataac gatccacgaa tgacgatcca cgaatgacga tccacgatcc
     1081 acgatccacg tatgcaactc aatcccaaac aacaggaagc cgtggagcac cgtgacgggc
     1141 ctctgctcat tctggcaggc gccggttccg gaaaaaccag ggttctggtc gaacgcatcg
     1201 tgcgtctgat gcaaactggc ggcgtcaacc cctggaacat catggccgtt acgtttacca
     1261 acaaggcggc caaggaaatg aaggagcgcg tggcccagtc catgggacag agagccaatg
     1321 acctctggat ggcaaccttt cactcgtttt gtctgcgttt gttgcgccgt cacgctgagg
     1381 tgctcaatta tcacactcat ttcacgattt atgatgatct tgatcagcga tccctgatca
     1441 aaaaaattct caaagacatg caactcagtg ataccgtgta caaaccagcc agcatccagt
     1501 accacatcaa caaggccaag aacgacggca tcaaggccga agatttccct tcgggcgggg
     1561 attttttcct gaaaaaagtt ggcgcgattt tcgccgaata ccagaagcat ctgattctga
     1621 atcaggccat ggattttggt gatctgatcg tcaacgcctt gcacctcttt ggcaagagtc
     1681 ccgaaatact gtcaaagtat cagaatcaat tccagcacat tttggtcgac gaatatcagg
     1741 acacaaacaa gagccaatac gcgctgattc ggttgttggc cgataaatgg aaaaacgtct
     1801 gtgtggtggg ggatgatgac cagagcatct acaaatttcg tggcgccgag atccgcaaca
     1861 ttcttgactt ccagagagac tacccacaag cgaaggtgat ccgtctggaa cagaattatc
     1921 gttccacgca gaatattctg aatgcctcca atgcggtcat ccggggcaac acagaacgca
     1981 tgggcaagga attgtggacc cagaacgggc agggcgaatg catcagaatg tttggcggac
     2041 aaactgatca cgacgaagcc gagtttgtct gttcctccat catgtcacac agggaaaaat
     2101 acagactcgg ggacatggcg attttttatc gcacaaacgc gcagagccgt atactcgaag
     2161 attccctgcg caaacacaaa attccctatc gcatcttcgg tggcatcaag ttttatgaca
     2221 gggccgaaat caaggatatg atgtcttatt taaagacgtt ggtgaatccg tccaacacga
     2281 tttcgctcaa gagaattctc aacacaccgg cccgtggcat cggcaaaacc acggttgaac
     2341 gtatcgacgc ggcggcagat cgggaaagcg cctgtttctg ggacgtgatc agttcggtgt
     2401 ccgatcctcg ctgggccctt gagctcaaca ccggcacaat gaccaaaatc gaggactttg
     2461 taaaactcat cgcccgtctc aatcagg
//
LOCUS       DATDUN010000016         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_167165, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000016 DATDUN010000000
VERSION     DATDUN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167165"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VJC18_00185"
     CDS             complement(<1..232)
                     /locus_tag="VJC18_00185"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HLD43823.1"
                     /translation="MYPIIIHVGSLVVPAFFFMTMLGVLSCTFYLYFRAPRLGFSQVV
                     VLDCGIIGAIFGILGARLFHVFFEYFWYYKEDP"
     gene            complement(259..828)
                     /gene="lspA"
                     /locus_tag="VJC18_00190"
     CDS             complement(259..828)
                     /gene="lspA"
                     /locus_tag="VJC18_00190"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HLD43824.1"
                     /translation="MKSKLSIVIIIAVLIFVADHATKWMIVNQIPLGDRVPVIPNIFD
                     IVHGRNTGAAFGFLSDWNSPFKDWFFYFVGFLALIFLYYYVKSVKKTDTLILVSLAMI
                     LGGASGNLVDRVLRGGVVDFLSVHYFHKICSFQIFNYQITFPLDWPAFNVADSAISVA
                     VVMLLIEQFRGARRERLDHGLIKSDSSSS"
     gene            complement(825..1841)
                     /locus_tag="VJC18_00195"
     CDS             complement(825..1841)
                     /locus_tag="VJC18_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLD43825.1"
                     /translation="MKKIVLLVVILASLLVTKAYAAEIIATVDDRQINESEVMSLASP
                     RMIKVQAQIYDIKRAAVDQLIQDFLMGREAKKRGLSVEQLEKDIRKKAETVSEGEAQV
                     VYNMNKARFQNKSFDEVKTMIMAQLKMQKQQLAVDDFVQELRGKAKVKINLQRPRYTV
                     SPDDDPGQGNKNAPIVLIEFSDFQCPYCKRVRPVLKRLMSQYKDKLYYVFRDFPLSFH
                     KQAKDAANATQCAAEQGKYWEFNEKIWDGQREIFNKELSHEDFLGKFREISKALGLAA
                     DKFDSCMTGKKYYAEIDKDQADGLAAGVTGTPAYFINGVFLSGAQPYESFVEVIEDEL
                     QLKE"
     gene            2027..2490
                     /gene="ruvC"
                     /locus_tag="VJC18_00200"
                     /pseudo
     CDS             2027..2490
                     /gene="ruvC"
                     /locus_tag="VJC18_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
BASE COUNT          701 a          650 c          515 g          624 t
ORIGIN      
        1 aagggtcttc cttgtaatac caaaaatatt cgaaaaatac gtggaagaga cgcgctccca
       61 aaataccaaa aatcgcccca atgataccac aatccagcac gaccacttga gaaaaaccca
      121 gtcggggtgc cctgaaatac agataaaagg tacagctcaa cacacccaac atggtcatga
      181 aaaagaatgc ggggaccaca agactgccaa catgaatgat aataggatac ataaaatacc
      241 gctttgcaaa acccctgtct acgagctaga ggagtccgac tttatcaaac catgatctaa
      301 acgctctcgt cgtgcccccc gaaactgctc gattaacaac atcaccacgg ccactgatat
      361 ggcactatcg gcaacgttaa aggctggcca gtccaaggga aaggtgatct gataattgaa
      421 aatctgaaag gaacatattt tgtgaaaata atgaaccgac aggaaatcca caactccacc
      481 acgcaagacc cgatcgacca ggttcccgct ggccccgccc aaaatcatgg ccagtgatac
      541 cagaatcagg gtgtcggtct ttttaacgga cttgacgtag taatacaaaa atatcagcgc
      601 caaaaacccc acaaaataaa aaaaccagtc tttgaaaggg gaattccaat cactcaagaa
      661 gccaaaggcg gctcccgtgt tgcgaccgtg cacgatatcg aagatgttgg ggatcacggg
      721 cacacgatcc cccaagggaa tctgattaac gatcatccat ttcgttgcgt gatctgctac
      781 gaaaatcaaa acggcgatga tgatgactat ggaaagtttg cttttcactc tttcagttgc
      841 aattcgtctt cgatgacctc gacgaaagac tcatacggtt gcgcacccga caagaagacc
      901 ccgttgataa aataagccgg cgttcccgtg acaccagccg ccagaccatc cgcctgatcc
      961 ttatcgattt cggcataata cttcttgccc gtcatacagc tatcaaactt gtctgccgcc
     1021 aaccccaggg ccttggagat ttctctgaac ttgcccaaaa aatcttcgtg acttaattct
     1081 ttgttgaaaa tttcgcgttg tccgtcccat atcttctcgt taaactccca atactttccc
     1141 tgttccgctg cgcactgtgt ggcgttggcc gcatccttgg cctgtttgtg aaagctcagg
     1201 gggaagtctc tgaacacgta gtacaacttg tctttgtatt ggctcattaa cctcttgaga
     1261 acaggacgaa cccttttgca ataaggacat tgaaagtccg aaaattcgat caaaacaatg
     1321 ggggcgtttt tgttgccctg accaggatca tcgtctggag acaccgtgta gcggggtctt
     1381 tgcaggttaa ttttaacctt ggccttgccc ctcagttcct gaacaaagtc atccaccgcc
     1441 aattgttgtt tctgcatctt gagttgtgcc atgatcatgg ttttgacttc gtcaaacgac
     1501 ttgttttgaa accgcgcctt gttcatgttg tagacaacct gcgcctctcc ctcactgacc
     1561 gtctccgctt tttttctgat gtccttctcg agctgttcaa cactcaaacc tctttttttg
     1621 gcctctctcc ccatcaaaaa atcctgtatg agctgatcaa ccgccgcccg tttgatatcg
     1681 tagatctggg cctgcacctt gatcatgcgg gggctggcca ggctcatcac ttcggattcg
     1741 ttgatttgac gatcatcgac cgtggctatg atttctgccg cataagcctt tgtgaccaat
     1801 aaactcgcca aaataacaac aagcaacacg attttcttca taggagttcc ctagtagtca
     1861 aaacatcctc ggaaacgtga ccaacagggg tcactcaaat ctctctggat gagtcctgtt
     1921 cttacacaaa tttttccacg tgactagtac aaaaagcaac cagacacaaa ggaattgaga
     1981 gaggttcctt tcttgattaa tcaacttatc ctgtatatga ttcttcatga taattcttgg
     2041 tatcgatccc ggcagccgca tcacaggttt tggactaatc gacaaatcag gcaaccaatc
     2101 aagacacgtc gaaaacggca ccatttattt ggagaagcaa ggttcttttt ctgcccgcct
     2161 ggtttttctc tatcaggaaa ttcaagcgat cattcaggcc ttcaacccga caattcttgc
     2221 catcgaaaat attttttaac ataaaaatcc aaagtctgtt cagaagttag gtgaggtgcg
     2281 tggcgtggcc atgctgtcag gcgccatcgc caaactgcaa atttgcgaat acactcccct
     2341 ggaaataaaa aaagccatca cgggttatgg cagggccagc aaagaacagg ttcagtacat
     2401 gattggcaaa ctcctcaatc tcaaggaccg ggcagaggaa aatgccagcg acgccctggg
     2461 ggttgccctt tgccacgccc ataccatgag
//
LOCUS       DATDUN010000017         4738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_1143, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000017 DATDUN010000000
VERSION     DATDUN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..160
                     /locus_tag="VJC18_00205"
     CDS             32..160
                     /locus_tag="VJC18_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLD43826.1"
                     /translation="MPDFAHSDPADQIIVATAREEGAVLITKDRRLTSYEHIKTLW"
     gene            250..1113
                     /locus_tag="VJC18_00210"
     CDS             250..1113
                     /locus_tag="VJC18_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLD43827.1"
                     /translation="MKPVCIKELTRVNHADLRRCWCRAFSDYDVPLQVSDEELHIRLT
                     QASYSPELSCGAFVAGRLVGFWLTGLRCIDGALVGYDAGTAIIPEYRGKGVSKQLSDY
                     LDLLLLRKKVSKYILEVFCNNAKALRLYQKKGFSVLETVQTFRATPFPDHSLANHSYS
                     IRPCSLEEAFQQRRLLDYTPTWQNAWEGLRSVERSTICVFAYEGDSLAGYAIYQPVLR
                     RIAHMGVTPNSSHQQIVGAMINYFGKQSQRHDKLEMVNVHPASRLPVVLESFGLRHFV
                     SLYVMERNLGV"
     gene            1196..1780
                     /gene="pgsA"
                     /locus_tag="VJC18_00215"
     CDS             1196..1780
                     /gene="pgsA"
                     /locus_tag="VJC18_00215"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HLD43828.1"
                     /translation="MLFSIPNIITYIRIVLIPVLVVLLLLVSPSKSDEANFQLCFWAM
                     IVFTIAGVSDLFDGYYARRYGAVSKMGKFIDPMADKLIHMTAMVMMIPLGRIAAWVVV
                     VLLFREIFITGIRAVAAAEGVVIDAAQWGKYKTAWLNVGLGAMILYYPLFKGTVFEVD
                     SYTFGVVCVVVGAFFAIASAVQYSIRFFKIVGRQ"
     gene            1788..3512
                     /gene="gspE"
                     /locus_tag="VJC18_00220"
     CDS             1788..3512
                     /gene="gspE"
                     /locus_tag="VJC18_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HLD43829.1"
                     /translation="MHNRSIGNILLEYTALTPQQLEESLTLGREKSLRLGDAILQLGF
                     LSQEDILKAISFQLGLEYLDELSPDEIPNDLINQVPINFAKKNEVIPIRREEGQIVVA
                     IVDPENHTALDDLRILFDSPVKPIVAGSHWVVDAINSCYNRTPENDAVMSELDEENTD
                     MIDDFEEVEDLLDSDDEAPIIRLVNQLLVRSVKQKASDIHIEPFEKDLVVRYRIDGTL
                     YEIMRPPKKAQNSIISRIKIMADLNIAEKRLPQDGRIRIKLAGKDIDIRVSTLPTSYG
                     ESCVMRLLDRSKVLQSLGTIGFFQEDLGTIRDLITHSHGIILVTGPTGSGKTTTLYAC
                     LAELNSPDVKIITVEDPVEYQIPGINQCQVNPKIDMTFAAALRSILRQDPDVVMIGEI
                     RDKETAEIAVQASLTGHLVISTLHTNDTSTTPTRLIDMGVEPFLVGASLIGIIAQRLV
                     RRVCQNCRVPQPLTVKEIGELNLSEKDVLEKTIYRASGCPECLDTGYSGRTAIHEVLT
                     ISDKVRDLINANADAVQIKKAAMREGMKTLREVAILKMLNGETSFEEVMMLTQEDVQR
                     EKAVVQTN"
     gene            3703..>4738
                     /locus_tag="VJC18_00225"
     CDS             3703..>4738
                     /locus_tag="VJC18_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLD43830.1"
                     /translation="MPVFEYKGVDAKSKNVNGVVDAESERAARAKLRKQKIFPTKLIQ
                     QGTGPSLKSITLFNSVKVEDVAGMSRQLAILLNAKIPLIDAIEATADQVEHPEVRKAL
                     TEIKEKVSEGARLGDCMVHYPKVFDAIFIHMVKAGEASGSLDVVLNRLADFKEAQASL
                     KATVKSAMTYPILMVVVSVGLLGYLFTSVVPKITKIIEKQKTVLPLPTRIVMSITDII
                     TGYWYLVAIGLVVVVLLFLSWKKTPAGRERIDEWKLRAPVFGALNIKIAVSRFARTLS
                     TMLNSGVQLLPGLEIVKNVLDNTVLARIIDSVMVSVKEGESLYDPLSRSGRFPSMFLH
                     MIRVGEKTGQL"
BASE COUNT         1161 a         1093 c         1257 g         1227 t
ORIGIN      
        1 ctatccctta ttggcatatc gttctacggt tttgcccgat tttgctcact ccgaccccgc
       61 tgatcagatc atcgttgcta cggcgcgaga agagggtgcc gttctcatca caaaggacag
      121 acggttaacg tcgtatgagc atatcaaaac gttgtggtga attggttcaa aatgttttcg
      181 tgaggtggtc tgtcagttgg tcactgaacg caacataata atcttgggag ctggggagtc
      241 cacaattgca tgaaaccagt ttgtataaaa gaacttaccc gggtcaatca cgcagacttg
      301 cgacgttgct ggtgccgcgc tttttcggat tatgatgtgc ccttgcaggt atcagatgag
      361 gaattacaca taagactgac tcaagccagc tattctcccg aattatcgtg cggtgccttt
      421 gttgctggca ggcttgtcgg cttttggtta acaggcttaa gatgtattga tggggcattg
      481 gtcggctatg atgctggcac ggcgatcatt ccagaataca ggggaaaagg cgtgtcaaaa
      541 caattatcgg attaccttga cctgcttttg ttgagaaaaa aagtttcgaa atacatcttg
      601 gaagtgtttt gcaacaacgc taaagcctta cggctctatc agaaaaaagg attttctgtt
      661 ttggagactg tccagacttt tcgagcgaca ccttttcctg accacagttt ggccaatcac
      721 agttattcaa tacggccgtg ctctttggaa gaggcttttc aacaacgccg tttgttggac
      781 tataccccta cctggcagaa tgcctgggag ggtttacggt ctgtagagcg atctacaatt
      841 tgtgtttttg cgtatgaggg tgattcgctt gcgggatacg caatctatca accggtttta
      901 agaaggattg cccacatggg tgtgacgccg aattcttctc atcaacaaat cgttggtgcc
      961 atgataaatt attttggaaa acaatcacaa aggcacgaca aattggagat ggtcaatgta
     1021 catccagcca gcagactccc ggtcgtcttg gaatcgtttg gcctgagaca ttttgtttcg
     1081 ttgtatgtga tggaaagaaa tttgggcgtt tgagaattaa acggacttga ttaatccccg
     1141 gtttcggcat accatgcccc gattcattaa acataacagt taactactca caattatgct
     1201 ctttagcatc cccaacatca tcacctatat ccgcattgtc ctgatccccg tgttggtcgt
     1261 gttgctcttg ctggtgagtc ccagcaaatc agacgaagcg aattttcagc tctgcttttg
     1321 ggccatgata gtctttacca tagccggagt ctcggacctg tttgatgggt attatgccag
     1381 gcgctacggg gcggtgagca aaatgggcaa atttatcgat cctatggccg ataaactcat
     1441 ccacatgacc gcgatggtca tgatgattcc gttgggacgc attgcggctt gggtggtggt
     1501 ggttttgctg tttcgcgaaa ttttcatcac agggatccgc gcggtggcgg ccgccgaagg
     1561 tgtggttatc gatgcggcgc aatggggcaa gtacaaaacc gcctggctta atgtggggct
     1621 gggggccatg atcctgtact acccgctctt taaaggaaca gtgttcgaag tggattctta
     1681 cacctttggg gttgtgtgcg tagtggtggg agcttttttt gccattgcga gcgctgttca
     1741 gtattcgatc cggtttttta aaattgtggg caggcaatga ggaagtcatg cacaatcgta
     1801 gcataggcaa cattttgctc gaatacaccg cgttgacgcc gcaacagctc gaagagtccc
     1861 tcactctggg gcgtgaaaaa agccttcggt tgggtgatgc gatcctgcaa ctggggtttt
     1921 tatctcaaga agacattctc aaggccatca gttttcagtt gggtctggag tatctggacg
     1981 aactcagtcc tgacgaaatt cccaacgacc tcatcaacca ggtgcccatt aattttgcca
     2041 aaaaaaacga agtgattccc attcggcgcg aagaaggaca gattgtcgtg gccattgttg
     2101 atcccgaaaa ccacacggcg ctggatgatt tgcggattct gttcgactct cccgtcaaac
     2161 ccattgtggc ggggtctcat tgggtggtgg acgccatcaa ttcctgttac aaccgcactc
     2221 ccgaaaatga tgccgtgatg agcgagctcg atgaggaaaa caccgatatg atcgatgact
     2281 tcgaagaggt agaagacctg ctggattctg atgacgaggc gccgatcatt cgtctggtta
     2341 atcagttgtt ggtccgttcc gtcaagcaga aggcgagcga tattcacatt gagcctttcg
     2401 aaaaagatct ggtggtgcgc taccgcatcg atgggactct gtatgaaatc atgcgccccc
     2461 ccaaaaaagc gcagaactcc atcatctcgc gcatcaagat catggcagat cttaatattg
     2521 ctgaaaagcg cctgcctcaa gacggacgca ttcgcatcaa actggcaggt aaggacatcg
     2581 atatccgcgt ttccaccctg cccacatctt acggggagtc gtgcgtgatg cgtcttttgg
     2641 accgcagtaa ggttttgcaa tctctgggca ccataggatt ttttcaggaa gatctgggca
     2701 cgatcaggga cctgatcact cacagtcacg ggatcattct ggtcacaggc cccacgggat
     2761 ccggcaaaac cacaaccctg tacgcctgcc tggcggaact caattctccc gatgtcaaga
     2821 tcatcacggt ggaagatcct gttgaatacc agattcccgg aatcaatcag tgccaggtca
     2881 atcccaaaat cgacatgacg tttgccgcgg ccctcaggtc cattttgcgc caggatccag
     2941 atgtggtgat gatcggtgaa attcgtgaca aagaaacagc cgagatcgcc gtacaggcct
     3001 cgcttacggg gcatttggtg atctcaaccc tgcacacgaa tgatacctcg accaccccga
     3061 cccgcttgat cgacatgggg gtggagccgt ttttggtggg ggcgtccctg atcggaatta
     3121 tcgctcaaag attggtgcgt cgtgtgtgcc agaattgtcg cgtgccacaa cccctgacag
     3181 tcaaagaaat cggagagctg aacctttccg aaaaagatgt gttggaaaaa acaatctatc
     3241 gcgcttcggg gtgtcccgag tgtttggaca caggctatag cggacgaact gccattcatg
     3301 aggtgttgac catttctgat aaggtgcgcg atctgatcaa cgcaaacgct gatgcggtgc
     3361 aaattaaaaa agcggccatg cgcgagggca tgaagacctt gcgagaggtg gctattctca
     3421 agatgttgaa cggcgagacg agtttcgaag aggtcatgat gctcacacag gaagacgtgc
     3481 aacgcgagaa ggcggtggtg caaacgaatt gaatatcgaa tagcaaactt tgcgacttcg
     3541 ctcaggacag gcaaggaatg tcgttgtgat ctgtcatccc tgcgaaagca gggatccatg
     3601 aaaatttaca tgtactggat tccagctttc gctggaatga caaatcattt cggtatttat
     3661 aattccttgt tcggtattca ttattcgata ttctaaaatc ctatgccagt attcgaatac
     3721 aaaggggttg acgccaaatc aaaaaatgtg aacggcgttg ttgatgccga gtcagaacga
     3781 gcggcccggg ccaagttgcg caaacagaaa atctttccta ccaaactgat tcagcagggg
     3841 acaggccctt cgttgaaatc catcacctta tttaattcgg tcaaagtgga ggatgtggcg
     3901 gggatgtcca gacagctggc cattttattg aatgccaaaa tccccctcat tgatgcgatt
     3961 gaagcgacgg ccgatcaggt ggagcacccc gaggttcgca aggcgctgac cgagatcaag
     4021 gagaaggtca gcgaaggcgc ccgcttgggc gattgcatgg tgcattaccc caaggtgttt
     4081 gatgcgatct tcattcacat ggtgaaggcg ggtgaggcat caggttcact ggatgttgtg
     4141 ctcaacagat tggctgattt taaggaggct caggcgtccc tcaaggccac ggtcaagagc
     4201 gccatgacct atcccattct catggtggtg gtgtctgttg gtttgctggg gtatttgttt
     4261 acgagtgtgg ttcccaaaat caccaaaatc atcgagaaac agaaaaccgt cctgccgctc
     4321 cccacgcgca ttgtgatgag tattaccgac atcatcactg gttattggta tctggtggcg
     4381 atagggctgg ttgtggtcgt gctcctgttt ttatcctgga aaaaaacacc tgccggccgt
     4441 gaacgcattg atgagtggaa gctgagagca cccgtctttg gtgcgcttaa catcaagatc
     4501 gcggtatcac gctttgcgcg tacgctcagc accatgctca atagcggggt tcagttgttg
     4561 ccgggtttgg agattgtgaa aaatgttctc gacaatacgg ttctggccag gatcatcgac
     4621 tcggtcatgg tcagtgtgaa ggaaggtgaa tctttatacg atcctttaag tcggtcgggg
     4681 cgttttccca gcatgttctt gcacatgatt agggtgggtg aaaaaacggg ccagcttg
//
LOCUS       DATDUN010000018         2060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_582654, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000018 DATDUN010000000
VERSION     DATDUN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582654"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VJC18_00230"
     CDS             complement(<1..586)
                     /locus_tag="VJC18_00230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLD43831.1"
                     /translation="MTDEITVGMAVTDAQKQGIYHLRYQVYVEDLCLMPEFADHQQKT
                     LAEPIDAHAKLFFAEAGGEIVGTMRINLGLDGPLEHESLYDLHKFVPYFPTCVAMCTK
                     FAVARKKRGSEVARRLIQDAYVYLRERGILFVFIDCWPHLLNLYEHLGFRRYRSNIRH
                     PEAGYMTPMVFVFDDVAHFELINSPLAPVAKQFPD"
     gene            complement(674..1468)
                     /locus_tag="VJC18_00235"
     CDS             complement(674..1468)
                     /locus_tag="VJC18_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43832.1"
                     /translation="MSFGQKMKYVFSLDGLLLMAGIFVVELLVMIVLDLFDFGGNDLI
                     RAVLDSVLLTILILPVVHKLFIIPVGTQRKKNIFFEMDLIRKNRALDTVNDLLIKTLE
                     SRNHKELSKVIAKKLKNISESDFCLIGMLDHKKQFEVLALSDTVLSGDDLAQEKERLR
                     KTDLTVFNGWLLPLINNEVVLVNDLANNIANLIFPSFLPESHSLLAIPLSMSDHKFGF
                     VILAKLTSPYSDAEKDAVLRMVPFLTSALDKKIEDTLALESKGVYI"
     gene            1659..>2060
                     /locus_tag="VJC18_00240"
     CDS             1659..>2060
                     /locus_tag="VJC18_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLD43833.1"
                     /translation="MIYQSITELIGKTPLLKLNKIGQNCPANILAKLENFNPCSSVKD
                     RIGQSMIEDAEKRGLIKSDTIIVEPTSGNTGIALAFVCAARGYKLVLTMPDTMSVERR
                     KLLKIFGAELVLTPGSQGMKGAIAKAEELVAS"
BASE COUNT          584 a          539 c          456 g          481 t
ORIGIN      
        1 agtcgggaaa ctgtttcgcc accggtgcca gtggggagtt tatcaactca aaatgggcca
       61 catcgtcaaa aacaaaaacc atgggggtca tgtaaccggc ttcggggtgc ctgatgttac
      121 tgcggtagcg ccgaaaaccc agatgctcat agagattgag cagatgaggc caacaatcaa
      181 tgaacacgaa taaaattccg cgctcacgca agtagacata ggcgtcctga atcaatctgc
      241 gagcgacctc actgccgcgt ttcttgcggg ccacggcaaa tttggtgcac atggccacgc
      301 aagtaggaaa ataggggaca aacttatgga ggtcgtagag agactcatgt tccaggggac
      361 catccaaacc cagattgatg cgcatggttc ccacaatctc tccccccgct tccgcaaaga
      421 acagtttcgc gtgtgcatcg atgggttcgg ccagcgtctt ttgctgatga tcggcaaact
      481 ccggcatgag acaaagatct tccacataaa cctggtatct cagatgataa atcccctgtt
      541 tctgtgcgtc ggtaacggcc attcctactg tgatttcgtc tgtcatatcc acctcctctg
      601 ttcattcact tgtaatggat tcacaagcac cattgaactt ctcgacaact gatctgtcat
      661 gccatcacca aaactaaatg tagacgccct tgctttccag cgccagcgta tcctcaatct
      721 ttttatccag tgctgaggtc aaaaacggca ccatacgaag cacggcatct ttttcggcat
      781 cgctgtaggg agacgtcaat ttggcgagaa tcacaaaacc aaatttatga tcactcatgg
      841 acaggggaat agccaacaaa gaatgggatt cggggagaaa tgagggaaaa atcagattgg
      901 cgatattgtt ggcgagatcg ttgacgagaa caacctcgtt attgatgagg ggcaatagcc
      961 agccattaaa aactgttaga tcagttttcc tgagcctctc cttctcctgc gccaaatcat
     1021 caccgctcag gaccgtgtca ctgagcgcca gtacctcaaa ttgcttttta tgatccaaca
     1081 tgccgattaa gcaaaaatcg ctctcagaaa tgtttttgag tttttttgcg atgacctttg
     1141 acaactcctt gtgattcctg ctttccagag tcttgatcag cagatcgttg accgtatcca
     1201 gggcccgatt cttccggatg agatccattt caaaaaaaat attttttttc ctctgtgtcc
     1261 ccacagggat gataaaaagt ttgtgcacaa ccggcagaat caatattgtc aacaagacgc
     1321 tgtcgagtac tgccctgatt aaatcattcc ccccaaaatc gaacaaatca agaacgatca
     1381 tgaccagcaa ctcaaccaca aaaatgccgg ccatcaacaa caagccgtcc aacgaaaaca
     1441 catacttcat tttttgccca aaagacatat gccccccacg cacggtcaaa acgcacaacc
     1501 gtacctcggg aatgtattcg aacctggcac aaaactcaag ctccgagatc agtgggatgc
     1561 aaaccgcgca ttgcctacat tgattgatga caattgatgt tatgagtgga gtgtgttaat
     1621 tgtctccagc acagtcattt tttttaggag accccccaat gatttatcaa agcattaccg
     1681 agctcatcgg caaaacacct cttctcaaac tcaataaaat aggtcagaat tgtcctgcca
     1741 acattttggc aaagctcgaa aatttcaacc cctgcagtag cgtcaaggac cgcattggcc
     1801 agagcatgat cgaggacgcg gaaaaaaggg gactcatcaa atccgacacg atcattgttg
     1861 aacccaccag cggcaacacc ggcatcgcgc tggcctttgt gtgcgcggcc agagggtaca
     1921 agctggtgct cacgatgccc gacaccatga gcgttgagcg acgcaaactc ctcaaaattt
     1981 ttggggcaga actcgtgctc acccccggat cacaaggcat gaagggagcc atcgccaaag
     2041 cggaggaact ggtcgcctcc
//
LOCUS       DATDUN010000019         1433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_416505, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000019 DATDUN010000000
VERSION     DATDUN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416505"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VJC18_00245"
     CDS             <1..383
                     /locus_tag="VJC18_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43834.1"
                     /translation="KQMPSQEKPLADYANAAVNLLTKTGNLDKLYYQLVLERDKRKTD
                     ILNAFTKAGSLPFSIFNYLMFRLFPTITSLKRKFHWYNRTKYHDIEWDNSSYFLSVAS
                     CYRMLSKNGYLPHFIQTQLGEQTP"
     gene            708..995
                     /locus_tag="VJC18_00250"
     CDS             708..995
                     /locus_tag="VJC18_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43835.1"
                     /translation="MEFKKFSPEVVAELLIFLADHEDFNSINGLAPITKQEVKGLLKE
                     IADNLKEHLDKQVVSRKETVKQSDLTDNVYKVISRLTPHEENQLFKTFKIS"
     gene            1012..>1433
                     /locus_tag="VJC18_00255"
     CDS             1012..>1433
                     /locus_tag="VJC18_00255"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HLD43836.1"
                     /translation="MKNPSSSSWVAYTDGASRGNPGLAGCGAYLCSPEGQEYREFRFL
                     DQVTNNQAEYEGLLLALTKLTELQADCIMLRADSELMIKQLKGEYRVKNANMVPLFRK
                     AQVLLKNFSSVQFEHVPRAQNKIADGLANQAIDEHLLLA"
BASE COUNT          433 a          344 c          305 g          351 t
ORIGIN      
        1 ccaagcagat gccgagccag gagaaacctc ttgctgacta tgccaacgcc gctgtaaact
       61 tactaaccaa aaccggtaac ctcgataagt tgtattatca gcttgtgctt gaacgagata
      121 aaagaaaaac tgacatcctc aatgccttta ctaaagcagg aagccttcca ttctcgattt
      181 ttaactattt aatgttccgt ctattcccaa caattacgtc gttaaagagg aaatttcatt
      241 ggtacaacag aacaaaatat catgacatag aatgggacaa ttcatcatat tttttgtctg
      301 ttgccagctg ttatagaatg ttgagcaaga atggttattt gccccacttc attcaaacac
      361 agcttggcga acaaactccc taatttgagc ctcccgccga aaggttccac ataccttgaa
      421 aggtgctaca taccttgaaa ggtaccacat accttgaacg aagcctccat gaaatatgaa
      481 gaaaaaaaac cggaaattta gagcaacttt tgccaagtta gatcgatagt ggagtggggc
      541 gtgtctgaac tcattgtacg gaatgaaaac aaatcatgcc gctctcaata agaggttgcc
      601 aaggccaaaa acgcaaccac atgtttttat taataattaa tttggcctca gcagttgaca
      661 ttcgttaaaa aatgagtaca caatgagtca attatgggga actgactatg gaatttaaaa
      721 aattttcacc agaggttgtc gccgaactac tgattttttt ggctgaccat gaggatttca
      781 attcgatcaa cgggctggcc cccatcacca aacaggaagt caagggcctg ctcaaggaaa
      841 tagcggacaa cctcaaggaa cacctcgaca agcaggtcgt gtcgcgcaag gaaactgtca
      901 agcaaagcga cctcaccgat aacgtttaca aggtcatttc cagactcact cctcacgaag
      961 aaaatcaact gttcaagact tttaagattt cgtaaacatc tgttgcttgc tatgaagaat
     1021 ccatcatcat cgtcctgggt cgcctacacc gacggtgcca gtcgcgggaa tcccggtttg
     1081 gcaggctgtg gcgcttacct gtgttcaccc gaagggcagg aatatcgtga gttcaggttt
     1141 ttggaccagg tcaccaacaa tcaggccgaa tacgaaggtc tcctgttggc actgaccaaa
     1201 ctcacagagc tccaggccga ctgcatcatg ttgcgcgccg actctgagct catgatcaaa
     1261 caactgaaag gggagtaccg cgtaaaaaac gccaatatgg ttcccctctt tcgaaaagcg
     1321 caggtgttgc tcaaaaattt ctcgtccgtg cagttcgagc atgtcccccg cgcgcagaac
     1381 aaaatcgccg atgggctggc caatcaggcc attgatgaac atttgttgct cgc
//
LOCUS       DATDUN010000020         1543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_167263, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000020 DATDUN010000000
VERSION     DATDUN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167263"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VJC18_00260"
     CDS             complement(<1..697)
                     /locus_tag="VJC18_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43837.1"
                     /translation="MKTWPGLILMCLLFVLSSCKEMAIVHDLSERDANEIIVMLAKNN
                     IKASKIKEIRNQEVFWVIQTSSSDELQARSILVANNLPRIRQGGLEGICKDTGLILTP
                     KAERCRELLAYKGEIINSLESIPGVVSADVVLNLPEKEDFPDPNAPQPRATASVTIQY
                     LADANVKTRLTEAKVQEFVANSITDLDPRDVAVVISYFAQRIDNDLSNNGQNGPPNDP
                     DKTTPQNAQGDEGV"
     gene            complement(727..1182)
                     /locus_tag="VJC18_00265"
     CDS             complement(727..1182)
                     /locus_tag="VJC18_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD43838.1"
                     /translation="MIDVPREDVIVMLEAGYIYLAMKKFKEAKQIFEGICELAPKHDV
                     PLVGVANVYFAQGKYLEAIRILKNTIKGRPQSAFAWAHLGEAQLFYGKRDEALKNLQK
                     ASELESGKDGKSGEFARSLLDLMNLGYDPREYRKVFKKFVEEQKQKQET"
     gene            complement(1283..>1543)
                     /locus_tag="VJC18_00270"
     CDS             complement(1283..>1543)
                     /locus_tag="VJC18_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43839.1"
                     /translation="GSLGTSYSGGYYTGSGSGSSMYSTTGTGDDLSSNVESIISDTAT
                     SQAYLIGIQAQLGQQQSTFTAVSNALNVKHSMEKSAIQNFRV"
BASE COUNT          350 a          410 c          358 g          425 t
ORIGIN      
        1 tgacaccctc atcaccctgc gcgttttgcg gggtcgtttt gtcggggtcg ttcggtggcc
       61 cattttgtcc gttgttggaa aggtcattgt cgatcctttg ggcaaaataa ctgatgacca
      121 ccgccacgtc acgtgggtcc aaatcggtga tgctattggc aacaaattct tgaaccttgg
      181 cctctgtcaa gcgagtttta acattggcat ccgccaagta ctggatcgtc accgatgccg
      241 tggccctggg ttgcggcgca ttgggatcgg ggaaatcctc tttttcgggg aggttgagca
      301 ccacatcagc cgacaccact cccggaatgg attccagtga attgatgatt tctcccttgt
      361 aggccaagag ttcgcgacaa cgttcggcct tgggcgtgag gatgagtccc gtgtctttgc
      421 aaatgccttc cagccctccc tgtctgatgc gcggcagatt attggcgacc aatatactgc
      481 gggcctgtag ttcgtcggac gaactggtct gaatcaccca gaagacctcc tgattcctga
      541 tttccttgat tttgctggcc ttgatattat ttttggccaa catgacaata atctcattgg
      601 catcgcgctc gctcagatcg tgcacaatgg ccatttcctt gcaggacgac aagacaaaca
      661 gcaaacacat taaaatcaag cctggccagg ttttcataac cgtgttttgt cgcatgtgat
      721 gggccctcac gtctcctgct tctgcttttg ctcttccaca aattttttga ataccttacg
      781 atactctctg ggatcatagc ccaggttcat caggtcgagg agcgatctgg caaactcgcc
      841 tgatttgcca tctttgcccg attcaagctc tgaggccttt tgcaagtttt taagagcttc
      901 atcccgtttg ccataaaaca actgtgcctc tcccaaatgc gcccaggcaa aggccgactg
      961 gggtctgccc ttgatggtgt ttttgaggat acggatagct tccagatatt taccctgtgc
     1021 aaaataaaca ttggccacac caactaaagg cacatcgtgc ttgggcgcca gctcgcagat
     1081 accttcaaaa atctgttttg cctctttgaa tttcttcatg gccaaataaa tgtaaccagc
     1141 ctccaacatg acaatgacat cttcgcgtgg tacatcaatc ataatttccc cccaaaaaaa
     1201 caacgacaag ccgccccgtt ggggcagcct gtcagattat ccattcaaga ttatatttac
     1261 taaccatcac tcaatcaacc tgttacaccc taaaattctg gatcgccgat ttttccatgc
     1321 tgtgttttac gttcagcgca ttggacacgg cggtaaaggt ggattgctgt tgtcccaact
     1381 gggcctgaat cccaatgagg taggcttggg acgtcgctgt gtcggagatg atgctctcca
     1441 cgttgcttga aagatcgtcg ccagtgcctg ttgtgctgta cattgacgaa cctgagccag
     1501 agcctgtgta ataaccgcca gaatagcttg tccccaatga gcc
//
LOCUS       DATDUN010000021         2419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_42789, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000021 DATDUN010000000
VERSION     DATDUN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42789"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VJC18_00275"
     CDS             complement(<1..219)
                     /locus_tag="VJC18_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43840.1"
                     /translation="MMRLSHTSSRVACARALVFLVCVFFLAVMLITPCFAKTSYKKIF
                     KQVSRHEEVYGRDDFYASLNWYATYQDAA"
     gene            complement(261..629)
                     /gene="dksA"
                     /locus_tag="VJC18_00280"
     CDS             complement(261..629)
                     /gene="dksA"
                     /locus_tag="VJC18_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HLD43841.1"
                     /translation="MKKTELKKFKELLEHRKEDIIKQAGHVGEPLYSLKEEELFDEVD
                     LASTESDQSMSIRLKDRERTLLKKINKVLQKIEDGEYGICEKCGEEISVKRLEARPVT
                     DLCIRCKEEQERVERSYADE"
     gene            complement(761..1120)
                     /gene="rplT"
                     /locus_tag="VJC18_00285"
     CDS             complement(761..1120)
                     /gene="rplT"
                     /locus_tag="VJC18_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HLD43842.1"
                     /translation="MARAKRGFKARRRRKAVLSKAKGYRGGQSKLFRTAAEKVRRAEK
                     FSYRDRKVKKRNFRSLWIVRISAAVKELGLSYSRFINGLKLVGSELDRKALSQLAITQ
                     PKAFAKLVDQVKTKIAA"
     gene            complement(1225..1422)
                     /gene="rpmI"
                     /locus_tag="VJC18_00290"
     CDS             complement(1225..1422)
                     /gene="rpmI"
                     /locus_tag="VJC18_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005211352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HLD43843.1"
                     /translation="MPKIKTCRGAAKRFKLTKKGKVKYKKAFKSHILEPKSKKMKRQA
                     RRASYIHPSDEKNVKQMLPYG"
     gene            complement(1487..2299)
                     /locus_tag="VJC18_00295"
     CDS             complement(1487..2299)
                     /locus_tag="VJC18_00295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLD43844.1"
                     /translation="MIQDHFWFTFGPLFFVNAFIVVSLVVFIVISRHRPKTAEIEERH
                     SSIILNKWIREFWMWLLEPIFRFFIFFHISPNAISITGCVVAIFSAAAFAVGHFGLGG
                     WLMVLGASLDFFDGRVARATNRCTQAGSFIDSCLDRVSEGLTLTGLAFFYRDSFIFWV
                     VMLAYLGSMLTSYTKAKGETMGVAYSGGMMQRPERIAYLGAGGILAPVFAYALFPLLF
                     PRFPQLTLLGLVGYVYLIPLFFVTVLSNVAALNRMINIMRILDKQKVTGSKM"
BASE COUNT          653 a          602 c          516 g          648 t
ORIGIN      
        1 agcggcatcc tggtaggtcg cataccaatt cagggaggcg taaaaatcgt cccgcccgta
       61 aacttcttcg tgacgcgaga cctgcttgaa aatctttttg taggaagtct tcgcaaaaca
      121 cggtgtgatg agcatcacag ccaaaaaaaa cacacaaacc agaaaaacca acgcacgcgc
      181 gcatgccact ctggatgatg tgtgtgacag tctcatcatg atatacagga ctaattaagt
      241 taagggcacc tcaaaaatgt ttactcatcc gcgtaagaac gctccaccct ctcttgctct
      301 tccttgcacc ggatgcacag atcggtcacg ggtctggcct ctagcctttt gacactgatc
      361 tcctctccgc acttttcaca gatcccatac tccccatcct cgattttttg caggacttta
      421 ttgatctttt tgagcaaagt gcgttcacgg tccttcaaac gaatgctcat gctctgatcg
      481 gattccgttg aggccagatc gacttcatcg aacaattctt cttctttgag ggaatagagt
      541 ggctcgccca cgtggccggc ctgcttgatg atatcttcct tgcgatgttc caataattcc
      601 ttgaattttt tcagctcagt ttttttcatg gcccctgctc cctttgatat actccaacaa
      661 cttgacacag cagaattgcc cattcacaat tctttgtcaa tcgcgaattg taacccacaa
      721 gacagcaaat attcaactca caaaaaaaat gaatgtttgt ttaagctgcg atctttgttt
      781 taacctgatc aaccaatttg gcaaaggctt ttggttgggt gatggccaac tgagacaggg
      841 ccttgcgatc aagttcagac ccgaccaact tgagcccatt gatgaagcgc gaatagctca
      901 acccgagttc tttcacagcg gcgctgatac gcacgatcca aagagatctg aagttacgtt
      961 ttttgacttt gcggtctcgg taggaaaact tttctgcacg acggaccttt tcggcagctg
     1021 tcctaaacaa cttggactgt cccccgcggt aacctttggc tttactcaaa acagcttttc
     1081 ttcttctgcg ggccttaaaa cctcttttgg ctcttgccat tttttctcct tgagcttgct
     1141 tggcgtttgt ctatttttta agacaactct tcgggtgcca aaacgaagcc atgttattgt
     1201 gctaggcgtt tggaacacag cacgttatcc atagggcaac atctgtttga catttttttc
     1261 gtcagacggg tgtatgtatg atgcccggcg ggcctgccgc ttcattttct tggatttggg
     1321 ttcaagaata tgtgacttga aggctttttt gtatttcact ttaccttttt tggttaactt
     1381 gaatcttttg gccgctccac gacatgtctt aatctttggc atacaatcct ctcagttaat
     1441 aaggcccgcc tatttaggaa aatcaagaca agattgcaac gattatttac atttttgacc
     1501 ctgtaacttt ctgtttgtcc agtattctca tgatattaat cattctgttc agggcggcga
     1561 cattgctgag taccgtcaca aaaaacaatg ggatcagata gacatagccc acaagaccca
     1621 ataaggtcag ctgtgggaaa cgagggaata gaagggggaa taatgcgtaa gcgaacacgg
     1681 gggccaggat gccacctgct ccaaggtaag ctatgcgttc cggtctttgc atcatgccgc
     1741 cagaatacgc aacccccatg gtttcgccct ttgccttggt gtaactggtg agcatggaac
     1801 ccagataggc cagcatgacg acccagaaaa taaatgaatc acgataaaaa aaggcgagcc
     1861 ccgtcagagt cagtccttcg gacaccctat ccagacagga gtcgatgaag gaccctgcct
     1921 gagtacaacg attggtcgcg cgagccacgc ggccatcaaa aaaatcaaga ctggccccca
     1981 aaaccataag ccatcctcct aaaccaaaat gaccgaccgc aaaggccgcg gccgaaaaaa
     2041 tagccaccac acaacccgtg atggaaatgg cgttggggct gatgtgaaaa aaaatgaaaa
     2101 aacggaaaat gggttccaac aaccacatcc agaattcccg aatccatttg ttgaggatga
     2161 tggaggagtg gcgttcctcg atctcggcag ttttgggcct gtgacgtgag ataacaataa
     2221 aaacaaccaa cgaaaccacg atgaacgcgt taacaaaaaa taaggggcca aaggtgaacc
     2281 aaaaatggtc ttgaatcata tgacttctcg tgaatcgaaa tcagcttcaa agctcacatc
     2341 ttatacttcc caaaatcctc tggctccaga ttctccagaa tttctgtcca tttctgggat
     2401 ttgagctgat cttctgatt
//
LOCUS       DATDUN010000022         4521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_374965, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000022 DATDUN010000000
VERSION     DATDUN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374965"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VJC18_00300"
     CDS             complement(<1..491)
                     /locus_tag="VJC18_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43845.1"
                     /translation="MKPINPPTYFVQHLLYALAMCLATLGIILGTKMLTFFPATWGYL
                     SQGNLSEPIILGIIPTFNAALFLCYLCLYLNFEFYGFKTAFYGTLSVGSLIVVLFFIF
                     KFYPDYGLNASENTGDQTIKDLISMSEFKAVKMLLSVGSSFFVSFVFAAAIKKLTRNY
                     FMFL"
     gene            complement(540..1373)
                     /locus_tag="VJC18_00305"
     CDS             complement(540..1373)
                     /locus_tag="VJC18_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43846.1"
                     /translation="MSRQKQFEYRISKNKNVIASGQRPRGDPQLVLNCLALAFILILI
                     PFPLSAKVPDLPTITPANFTMELARVTSTYEHIIQNNPKSLIRKFKKAHGAHHYRFSA
                     TETQLKNDYQKIVKQRNMLDPINQLKQSMKDMYLEHYSVTSDQLNAMDYEATLLAVGL
                     FKEIARLQKKYDSFLLPVIHNALLAVHAKKRGACKHWAEDLLIYLKPLKRRFFDVAWG
                     EAYPGKVMEHNTAVIIPKGRPFEDGLFVDPWRTSGRPYWSRVPDDHHYRWKQWAGFDR
                     N"
     gene            complement(1366..2793)
                     /locus_tag="VJC18_00310"
     CDS             complement(1366..2793)
                     /locus_tag="VJC18_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43847.1"
                     /translation="MLKINIIVILCCLFCTSCLNAPYSATPNLVLDESSLSTSLSPVA
                     TATVQQLSFVNGTTIAMEPYQSKQLYLTVTMSDGRTFHGITRELSSVITSEATDVIWY
                     SNDTSIVGISSSGRLIGRQSGTTTVIATVLNQSAVITVTVAPSASQDEIGDQPSSDDT
                     GNLENEEQEAENTDEADDEQAPPEVVEADTLNNLQITTNDTLWEVGDTVTLRCSLDFA
                     NSGLIQNVSSIFYLGSELQNVSWTSSDNAVVSINQGKASFVGYGISLITASYQDQSDQ
                     IEVVVKMKSEAPDDASDSFLNEGDVLTDNFGPNSGYGYADFPDIVYGPPANDLVSFGY
                     GGELTIELANYLIVDGIGPDFTVFENPFGGWEECASIAVSEDGTAYQTFDCDQYDADG
                     DGEYQGCAGKSVVNLGLADADYLDPDVSGGDVFDLEDLEEPMSTVRFVRITDIGLCGS
                     TADVPSSTSAGFDMDALAIVQGNNE"
     gene            complement(2800..4026)
                     /locus_tag="VJC18_00315"
     CDS             complement(2800..4026)
                     /locus_tag="VJC18_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43848.1"
                     /translation="MKTWLSFCVLLALISCGQISYQPASDDATESLDPDSNISDLPSH
                     RVFVFATDYQSSGQLYTATYEDTSTALSNSDLTMLGTEAKIKWHDNLLYILHAGAGFN
                     SISTDNLQIVDPYNTQAPFSTLAQYTTGNGTNPQDVVLSDQKAFISLYNPKNDPSNVD
                     AEGHPGDVIEMDTESGQITHRYSFYDDLADDGDRNAHASQMVLADDVLYVCLQDLESN
                     TFSATAPGKIGMIDTKQNEMLGIITLQSRNPYGIALDNAHTRLFITSTYDYQHQGSYG
                     GLEIVNLDSQQTELFIKDDVLGGYVERIRLSDEHAYAVISQFDLETFEFQSKLVAFPL
                     GITTADEITNFSIWGTDIRDIFYQQDTLWVSYRVISTTEGADTPMIKLFDTNTEEQLG
                     ETLYPLVAGVSLAGEE"
     regulatory      complement(4193..4421)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1121 a         1025 c         1203 g         1172 t
ORIGIN      
        1 agaaacatga agtaattgcg ggtgagcttt ttgatggccg cggcaaatac gaaggagaca
       61 aagaaactac tgcccacgct caagagcatt ttgacggcct tgaattcgct catcgaaatt
      121 aggtccttga tggtttgatc acccgtgttt tcagacgcgt tgagtccgta atcggggtaa
      181 aatttgaaaa taaaaaagag gacgacgatg agactgccaa ccgaaagcgt gccgtagaag
      241 gctgtcttga aaccataaaa ttcaaaattg aggtagaggc aaaggtagca tagaaacagt
      301 gcggcgttga acgtgggaat gatgcccaga atgatgggtt cgctcaggtt tccctggctc
      361 aggtaccccc aggtggccgg gaaaaaggtg agcatcttgg tcccgaggat gattcccagt
      421 gtggccagac acatcgccaa agcgtaaaga agatgctgga cgaaataagt gggggggttg
      481 attggtttca taggcgagta gcataaaaaa actcaatcca tttggcaaga agtggttgcc
      541 taattccgat cgaaccccgc ccactgtttc caacggtagt ggtgatcgtc ggggacgcgt
      601 gaccaatagg gacgaccgga ggtgcgccac ggatcgacaa ataacccgtc ctcaaatggg
      661 cgacccttgg ggatgatgac ggcggtgttg tgttccatca cttttcccgg ataagcttcg
      721 ccccaggcga catcgaaaaa tctgcgcttg agtggtttga gatagatgag caggtcttcg
      781 gcccaatgct tgcaggcacc gcgttttttg gcatgcacgg cgagcagtgc gttgtgaatg
      841 accggcagaa gaaaggaatc atattttttt tgaagtcgtg cgatttcttt gaaaagaccc
      901 acagccaaca gggtggcttc gtagtccatg gcgttcagtt gatcgctggt gaccgaatag
      961 tgttccagat acatatcttt catactttgc ttgagttggt tgatggggtc cagcatgttt
     1021 ctttgtttta caattttctg ataatcattt ttgagctgtg tttctgttgc tgagaagcgg
     1081 taatgatgcg caccgtgtgc ttttttgaat ttgcgaatga ggcttttggg gttattctga
     1141 atgatgtgtt cgtacgtcga tgtgactcgt gccagttcca tagtgaagtt cgctggagtg
     1201 attgtgggaa ggtcgggaac cttggcggag agtggaaacg ggatgagaat gaggatgaaa
     1261 gccaaggcaa gacaattcag caccaactgt ggatcgccac gtggcctttg gccactcgcg
     1321 atgacattct tattcttcga tattcgatat tcaaactgct tttgcctact cattgttccc
     1381 ctgtacgatc gccaacgcgt ccatgtcaaa tcccgcggat gtggatgagg ggacatcagc
     1441 ggtggaacca cagagaccaa tgtcggtgat gcgaacaaac ctgactgtgc tcatgggttc
     1501 ttcaagatct tccagatcga acacatcgcc accggacaca tcgggatcga ggtaatccgc
     1561 atcagccaaa cccagattga cgacggattt tcctgcgcaa ccttggtatt cgccatcacc
     1621 atcagcatcg tattgatcgc aatcaaaagt ctgatatgcc gtaccatctt cgctcacggc
     1681 tatgctggca cattcttccc atcctccaaa aggattttcg aaaacagtaa aatcggggcc
     1741 gatgccatcg acgatgaggt aatttgcgag ttcaatggtg agttctccgc cataaccaaa
     1801 cgaaaccaga tcattcgctg gggggccgta tacgatatcg ggaaagtctg cataaccgta
     1861 gccggagttg ggaccgaagt tgtcggtcaa tacatcaccc tcgttcagaa atgagtcaga
     1921 ggcgtcatcg ggagcttcag atttcatttt taccacgact tcgatttgat cgctctggtc
     1981 ctgataagag gcagtgatga gtgagattcc atagcctaca aaagaggcct taccctgatt
     2041 gatggagacg acagcgttgt cggaagatgt ccaggagacg ttttgcaatt ccgaacccag
     2101 ataaaaaatg gaagacacat tttgaatcaa accggaattg gcaaaatcaa gcgaacacct
     2161 cagagtgaca gtgtctccaa cctcccacag tgtgtcgtta gtggtgatct gtaaattatt
     2221 aagtgtatca gcctccacga cttcgggcgg ggcttgttca tcatcggctt catccgtatt
     2281 ttcggcttct tgttcttcgt tttcgaggtt ccccgtatca tcgctggaag gttggtcacc
     2341 gatttcatct tgagaggcgc tgggtgcgac agtcacagtg atgaccgcgg actgattgag
     2401 tactgtggcg atcactgtgg tggtgccaga ttggcggccg attaaccgac ccgaagatga
     2461 aatgccgaca atgctcgtgt cgtttgaata ccagatgacg tcggtagcct cagaagtgat
     2521 cacactgctc aattcgcgtg tgatgccatg aaacgttctt ccatcggaca tggtgaccgt
     2581 aaggtaaagt tgtttggact gatagggctc catcgcgatc gttgttccgt tcacaaaaga
     2641 cagctgttgc acagtcgccg tagcaacagg tgagagactg gttgatagag aactctcgtc
     2701 aagcacgagg ttgggcgtgg ctgaataggg cgcgttgaga caggaggtgc agaacagaca
     2761 acacaagatg acaatgatgt ttattttaag catgattgtc tattcttccc ccgcaagcga
     2821 cacgcccgca acaaggggat acagggtttc tcccaactgt tcctcggtat tggtatcaaa
     2881 aagtttgatc atgggtgtat cggccccttc tgtggtgctg atgacgcgat agctgaccca
     2941 aagggtgtct tgttgataaa aaatatcacg gatatctgtg ccccagatac taaagttggt
     3001 gatctcgtcc gctgtggtga tgccgagtgg aaacgccacc aattttgatt ggaattcaaa
     3061 ggtctccaga tcaaattgcg agatgacggc ataggcgtgc tcatcggaaa gtctgatgcg
     3121 ctccacatag ccgcctaaaa catcatcctt gatgaaaagt tctgtttgtt gactgtccag
     3181 atttacaatt tccaagccac cataggagcc ttggtgctgg tagtcgtaag tggatgtgat
     3241 gaataatctg gtgtgagcgt tgtcgagagc aattccgtag gggttacggc tctgcagggt
     3301 gatgataccc agcatctcgt tttgtttggt gtcgatcata ccgatcttgc cgggggcagt
     3361 ggccgagaat gtgtttgatt cgaggtcttg cagacagacg tacagaacat catccgccag
     3421 taccatctga gacgcgtggg cattgcggtc gccgtcatcg gctaggtcat cataaaagga
     3481 atagcggtgt gtgatttgtc ccgactctgt atccatttcg atcacatcgc cgggatgccc
     3541 ctcagcatcg acattgctgg gatcattttt gggattataa agagagatga aggccttctg
     3601 atcgctcagc accacatctt gcgggttggt tccattgccc gtggtgtatt gagccaacgt
     3661 gctaaagggc gcctgagtat tgtacggatc gacgatctgc agattgtctg tgctgatcga
     3721 attgaatccg gcccccgcgt ggaggatgta caggagattg tcgtgccact tgatctttgc
     3781 ctctgtgccc agcatggtca ggtcggaatt ggaaagagct gtggacgtgt cttcataggt
     3841 cgctgtgtag agttgtccac tggattgata atccgtggca aacacaaaga ccctgtgaga
     3901 gggcaggtcg ctgatgttgc tgtcgggatc caaagattct gtggcgtcat cgctggctgg
     3961 ctggtaactg atctggccgc aggaaatgag ggctaacaag acacaaaaag ataaccaggt
     4021 tttcatgatt ttgtcctcgc atatgattca ccaatgtcac tattcgattt ttttgggtcc
     4081 tgctccgggt cgctgttttt tgacgctctc cactcgcaag ctcgttccgc aggccgcgtc
     4141 aaaataacag ctccccgtat cacccaaaaa aaacatcaaa gtgatagtgc acaattttaa
     4201 ttggtgtgca ggtaggtctt ctgactcttg gatcttctca tcttcattcc ttcccaacca
     4261 tttgtcacaa tggccagtgg ttttgtatga agacaatccc caatcacagc ggcgggaccg
     4321 tgctggaatc ccggtttttc accggtcacc agcttccctg ttaacatcgg acacattccc
     4381 atgttaatcc tctacgagga acctgtctgg cacgtatgac aggaaaattc agtaactgtc
     4441 aatctgaaat acaaatcgag gtgtacgaca taaaagtggg tgaaatttgg aatagtgata
     4501 gcaccgcccc tcctgttgac a
//
LOCUS       DATDUN010000023         2677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_457925, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000023 DATDUN010000000
VERSION     DATDUN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457925"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="mtnA"
                     /locus_tag="VJC18_00320"
     CDS             <1..966
                     /gene="mtnA"
                     /locus_tag="VJC18_00320"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HLD43849.1"
                     /translation="EIYVTCNTWQDVAEAIKNMTIRGAPAIGVAAAMGVALGVQNQKS
                     KAKSKKESLGDFKHYVYQICHELFLTRPTAVNLQWALDRMKKILTNHQDKPIDELREL
                     LVREAVAIKNEDVETNQKMGAVGQALLSDGDTVLTHCNAGALATAGWGTAVGVVYSAI
                     QVGKKIKLIADETRPVLQGARLTAWEAQKCGLDVTVICDNMAASLMAKGLISKVIVGA
                     DRIASNGDVANKIGTYSVAIAAKYHNIPFYVAAPFSTIDPLTPTGKEIPIEERHPDEV
                     TTINGKRMVPEGVSVKNPAFDVTPAELITGIITERGIFCGPYWFK"
     gene            complement(1261..2433)
                     /locus_tag="VJC18_00325"
     CDS             complement(1261..2433)
                     /locus_tag="VJC18_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD43850.1"
                     /translation="MHKRHHLIFTCLLLLFFGLFLFTPPALGAEETQEPIDRDFTHYL
                     NLGVSLLDQENYLDSITAFQKALEISSDSAEVHLVMGMAYDGLGQYEKAIFHLKKAGQ
                     LSPQLSDAHVNLGLVYLKMDNVDEAIKTYYDALMIDPVNEKAYFGLGVAYQRMGRYEE
                     AIDAYSEAIMISPAFSDAYNNLGIALSHVGLYEEAITSYLIAIKIRPDLSEAAYNNLG
                     ITYQSVGQDDKAVEAYTNAILLDPEYTKAYCNLGHVRQQKGNYDEAMWAYQEALKIDP
                     AFADAHYGLGNAYGSTGEYEKAADSYLLAIRHNPKDARTYFNLGWSYGKLGQLDKELD
                     AYNQAISLKQDYAKAYLGRGLVYLKNGHKDMARQEYDVLKNLDAKMASSLYELLNQ"
BASE COUNT          736 a          661 c          576 g          704 t
ORIGIN      
        1 gaaatttacg tcacatgcaa cacctggcag gacgtagcag aggcgatcaa aaacatgacc
       61 attcgcgggg caccagccat cggtgtggcg gcggccatgg gggtggcatt gggagtccaa
      121 aatcaaaagt ccaaagctaa aagtaaaaag gaaagcttag gggatttcaa acattatgtg
      181 tatcaaatct gtcatgaact ttttttaaca cgccccacgg ctgtcaattt acagtgggca
      241 ctggatagaa tgaaaaaaat ccttaccaat catcaggaca aacccataga tgaactcaga
      301 gaactgctgg tcagagaggc cgtcgccatc aaaaatgagg atgtagaaac caatcaaaaa
      361 atgggcgcgg tcgggcaggc actgctcagt gatggcgaca ccgtgctcac acattgcaat
      421 gcaggggcct tggccaccgc cgggtgggga accgctgtgg gagtggtgta ttcagccatt
      481 caggtcggca aaaaaatcaa gctcattgcc gatgaaaccc gcccggtttt gcaaggcgca
      541 cgcctcacgg cttgggaggc acaaaaatgt ggccttgatg tgactgtgat ctgtgacaac
      601 atggcggcga gcctgatggc caaggggctc atcagcaagg tgatagtggg agctgatcgc
      661 attgcctcta atggtgatgt cgccaataaa atcggtacat actctgtcgc catcgcggcc
      721 aaataccaca acatcccatt ttacgtggcg gccccttttt cgacgattga tcctcttacc
      781 cctacgggca aagaaatccc catagaagaa cgccatcccg atgaagtgac taccatcaat
      841 ggcaaaagaa tggtccccga aggcgttagc gtaaaaaatc ccgctttcga tgtcacaccg
      901 gccgaactca ttaccggaat cattactgag agaggaattt tttgtggtcc gtattggttc
      961 aaataaaaaa agcgaaggat gaaacagccc ccctgcttca tgcccccgcc ttttttgtgt
     1021 tagcattatc gactttactg taattgacaa agctacttta cgtccaagca agcttggtgc
     1081 caatcccaaa gataaattat tcagctgatt ttttaatggg ttaacgattt aacattgaaa
     1141 ccattctcga aagaaagcac caactccgtc atatttttgt cgacaacgcg atatgtttat
     1201 caaattgaat tgccctaaac aatatttgac ggcatcgcca tttgacagca aagcgtgtga
     1261 ctattgattg agaagctcgt acaaggaact ggccattttg gcatcgaggt tttttaaaac
     1321 atcgtattct tgtctggcca tatctttatg accgtttttc aaatacacca accctcgtcc
     1381 cagataggcc ttggcatagt cctgttttaa actgatggcc tgattatagg catcaagctc
     1441 tttgtccaat tgtcccaact ttccataaga ccaccccaga ttaaaatagg tacgtgcatc
     1501 ttttggattg tgccgaatgg ccaataaata ggaatcagcg gccttttcgt attcccccgt
     1561 actgccatag gcattgccca atccatagtg agcgtcagca aaagcgggat caattttgag
     1621 ggcttcctga tacgcccaca tggcttcatc ataattcccc ttctgctgtc tgacgtgacc
     1681 caagttgcaa taggctttgg tgtattccgg gtccagaaga atcgcattgg tgtacgcttc
     1741 cactgccttg tcatcctgcc caacactctg atacgtaatg ccaagattgt tgtacgccgc
     1801 ctccgacaag tctggtctta ttttaatggc aatgagatac gacgtgatgg cctcttcgta
     1861 aagccccaca tgactcaagg ctatccccaa attattgtaa gcatccgaaa aagcgggact
     1921 gatcatgatc gcctcgctgt acgcgtcgat ggcttcttcg taacgcccca tcctctgata
     1981 cgccaccccc aatccaaaat aggccttttc gttgacggga tcgatcatca gcgcgtcata
     2041 ataagtcttg attgcctcgt ccacattatc cattttgagg tacaccagtc ccaggttcac
     2101 atgagcatct gacaattgag gactgagttg cccggccttc ttcaggtgaa aaattgcctt
     2161 ttcgtactgc cccaacccat cataggccat gcccatgacc aagtgaacct ccgcgctgtc
     2221 gctggatatc tcgagcgctt tttgaaaggc tgttatggaa tcgaggtagt tttcttgatc
     2281 gagtaatgac acacccaagt tcagataatg ggtgaaatca cgatctattg gttcctgcgt
     2341 ttcttcggcg ccaagggctg gtggcgtgaa aagaaacaaa ccaaaaaaca acaacaataa
     2401 acacgtaaaa atcaaatgat gtcttttatg catagtgttg ccaaatcaat gttgcttaat
     2461 ccacttttaa ttatagcctg tttctcccct gacgtctcca aaaaaatgtc gtttttttta
     2521 caccacaatc cagtggtgtc atacatttga cgcataaatg ttatattatt gattcattga
     2581 ttcattgttc ccccccccac aacccataac ccagaacccc agggtcaatt cttcaatttg
     2641 attaactaac gattttattg atttcttttc gatttga
//
LOCUS       DATDUN010000024         1775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_374995, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000024 DATDUN010000000
VERSION     DATDUN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374995"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..>1775)
                     /locus_tag="VJC18_00330"
     CDS             complement(40..>1775)
                     /locus_tag="VJC18_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLD43851.1"
                     /translation="FLKEGDIVYTVGADNTDEEVAQAQSQMSDVNLPMVVLINEGSAS
                     AAEIVAGALKNNNRAIVMGQKSFGKGSVQSLFSLRDGSSLKLTIAQYLTPGRESIQAV
                     GITPDIHLYPSIIGEDFFDLFEDSGYNENKLDAHLSNVNLVKTSQPIYNMTFLQPEDP
                     ETKESLYTSKIKDTDFAFQLALKILSLAKDSSKTNLLSQILPLLNEETKKQAVVIAET
                     LKTKKIDWSDGQTTAQPKLDLRFEISDETSKKISAIRAGTEITITVTVKNSSSQPAHQ
                     VITDIEALNPLLNHKEFVFGKVAPQEELSRFIKVKIPDEVVSYSENIKFLTYTEKTQT
                     APLTTHATTQFVEKQQPQFAYSYEVFDGQIPETMGNKNHIPEKGESVVLRVHLKNISQ
                     TDAVETTVNLKNKEGKFALIQEARKKLGLLKAQSETSCDLKFSIKNDFAKDKFQLQLF
                     GQDDKSRAGLSDTLTFSQIAPQKATPEPNHYYGAPTIIIKPETKQSKNKLFLSAQVAH
                     DQPIKDISVFATGKKVLYLNAEKGTQIRKKDITKNIALEDGLNSITIRARGETGLMSE
                     KSITLVYDKNQ"
BASE COUNT          356 a          373 c          471 g          575 t
ORIGIN      
        1 catttcttga ttgcttgatt gcttgattga ttcattgatt cattgatttt tgtcatacac
       61 cagcgtgatg cttttttcac tcattaagcc ggtttcgcct cgggccctga tcgtgatgga
      121 attcaagccg tcttccaacg caatgttttt tgtgatgtcc ttttttctga tttgtgttcc
      181 cttttctgcg ttgaggtaca acaccttctt gcctgtggca aaaacagaaa tgtccttgat
      241 gggttgatcg tgtgccactt gtgccgacag aaacagtttg ttcttgctct gtttcgtttc
      301 gggtttgatt atgatcgtgg gggcgccgta ataatgattt ggttccggcg tggctttctg
      361 gggggctatt tgggaaaacg tgagggtatc gcttagtccg gctctggatt tgtcgtcctg
      421 accaaagagt tgaagttgaa atttgtcctt ggcgaaatca tttttaatgg aaaacttgag
      481 atcgcaactg gtctcagatt gcgccttcag caggcctagc tttttgcgag cttcctggat
      541 gagggcaaac ttgccttcct tgtttttgag attgactgtg gtctcgacgg cgtcagtttg
      601 gctgatgttt ttgaggtgca cgcgcaatac aacagactca cctttttctg ggatgtgatt
      661 cttgtttccc attgtttcgg ggatttgacc gtcaaaaacc tcataggaat aggcaaattg
      721 aggttgttgc ttttccacaa actgagtggt ggcatgagtg gtgagcggag ccgtctgtgt
      781 cttttcggtg taggtcagaa acttgatgtt ctcggagtag ctaaccactt catcaggtat
      841 tttgaccttg atgaaccgag agagctcttc ctgaggggcc acttttccaa agacaaattc
      901 cttgtgattc aggaggggat tgagggcttc gatgtctgtg atgacctgat gtgctggttg
      961 tgaacttgaa tttttaacgg tgaccgtaat ggttatttcg gtcccagccc tgatggcgct
     1021 gatttttttg cttgtctcgt cgctgatttc gaatcgcaaa tcaagtttgg gttgtgctgt
     1081 ggtttgccca tcggaccaat cgatcttttt tgttttgagt gtctcagcga tgacgacggc
     1141 ctgttttttg gtctcctcat tgaggagtgg caggatctgg ctgagcaggt tcgttttact
     1201 tgagtctttg gccagactca aaattttgag cgccagttga aaggcaaaat cggtgtcttt
     1261 gatcttgctg gtgtacagac tctctttcgt ttccgggtct tcgggttgca gaaaagtcat
     1321 gttgtatatg ggctgtgaag ttttcacaag attcacattg ctcaagtggg catcaagttt
     1381 gttctcgtta tagccactat cctcgaaaag atcgaaaaaa tcttcgccaa tgatcgatgg
     1441 gtaaaggtga atgtccggcg tgatccccac ggcctgaatg gattcgcggc ccggagtgag
     1501 gtattgtgcg atcgtgagtt tgagcgaaga gccatcccga agactgaaca aggattgcac
     1561 actccccttg ccaaaagact tttgacccat gacgatggcg cggttgttgt ttttgagcgc
     1621 ccccgctaca atttcggcgg ctgaggctga tccctcgttg atgaggacca ccatgggcag
     1681 gttgacgtcg gacatttgcg attgggcctg ggcaacttct tcgtccgtgt tatcggcgcc
     1741 cacggtgtac acgatatctc cctctttcaa gaaaa
//
LOCUS       DATDUN010000025         2502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_1626, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000025 DATDUN010000000
VERSION     DATDUN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..678)
                     /locus_tag="VJC18_00335"
     CDS             complement(25..678)
                     /locus_tag="VJC18_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HLD43852.1"
                     /translation="MKQSTPSSHKEYTLGEEIANSITHGVGLALSIAGLVVLVTLSAI
                     HGDVWRVVSFSLYGFTLILLYLNSTLYHAITNKKAKKVFRILDHASIFLLIAGTYIPI
                     LLVTIRGVWGWTLFGIIWGLAILGVVFKSLFIHRFARLSVGLYVLMGWICVIAMHEIF
                     KKMDFGGIVLIASGGLFYTLGLVFYAWKKLPYNHMIWHLFVLGGSICHYFAFLLYVL"
     gene            972..2009
                     /locus_tag="VJC18_00340"
     CDS             972..2009
                     /locus_tag="VJC18_00340"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HLD43853.1"
                     /translation="MKVALLGYEYSGRKTLFSLLTGKVVPDHLIEGQVLEGMARINDA
                     RVDSLSKMYQPKKTVYVSTHFVLCPPISSGSKHDEWLKEARNADLLCVVVRDFASDDV
                     FHPLGRVDAQGDRQNIMSELILADLMLVETRLERIAKDKLRQAKAQNLLVEEGALHKC
                     KVVLDQEAPVSTVTFAKEEQAILNNLNLITQKSIMWCVNVAEGHANRAPGKPDEFIVS
                     AKIEKEIVGIENPEERQMFMQDLGLKDSGLNRLNAAAYHQLGLMSFYTVGPDEVRAWT
                     IKQGSLAPEAGGKIHSDIERGFVRVEVMKYDDLMAEKSEQKLKSLGKVYVKGRDYVIH
                     DGDICNFLFNV"
BASE COUNT          669 a          535 c          665 g          633 t
ORIGIN      
        1 atttttttgt tgtgcgcatg gtgtttacag cacatacaac agaaacgcaa aatagtgaca
       61 gatactgccg cccagcacaa acaggtgcca gatcatgtgg ttgtagggga gttttttcca
      121 ggcataaaag accaagccca aagtataaaa cagaccaccg ctggcgatga gcacgatgcc
      181 tccgaagtcc atctttttaa aaatttcgtg catggcgatc acacaaatcc agcccatgag
      241 cacataaagc cccactgaga gccgggcaaa tctgtgaata aagagagact tgaataccac
      301 acccaaaatg gctaggcccc agatgatccc gaatagtgtc cagccccaaa cgccccggat
      361 ggtgacgagt aaaatgggga tgtaggtgcc ggcaatgagc aaaaagatgg aggcgtggtc
      421 taaaatccga aaaactttct tggccttttt attggtgatg gcatgataca gggttgagtt
      481 cagatacagc agaatgaggg tgaatccgta gagagaaaaa ctcaccacac gccacacatc
      541 gccatggatg gccgatagag tgacaagtac gaccaaaccc gcgatgctca aggccagtcc
      601 cacaccatga gtgatgctgt tggcaatttc ctctcccagt gtgtattctt tgtgactact
      661 tggggtgctt tgtttcatgg gggttcggat acgaaatggg agggtgtttt gtaaagggag
      721 gattgcaaat tgcttttttg atgttaagta cgatatgtga aggcaacaga attttgtttt
      781 tacatcattg tttttgatcg agacatccct acgggtcctg ccttggcttg ctattttccc
      841 aatctcctcg cttcgcaagc tcgctcgtcc cgttgctacg ggtgcgattg ggaataatag
      901 cttcgccagt cacccgtaca gaatgtctcg atcaaaaaca ggtttactgt agtcagtgag
      961 ggagatgtgc catgaaagtt gcattgttgg gttatgagta ttctggtcgc aagacccttt
     1021 tttcacttct cacgggcaaa gttgtgcccg atcatttgat tgaggggcag gttctcgaag
     1081 gtatggcccg gatcaacgac gcgcgggtcg attccttgtc caaaatgtat caacccaaaa
     1141 agaccgttta cgtgagcacg cattttgtgt tgtgcccacc gatcagttcc ggatcaaaac
     1201 acgatgaatg gctcaaagaa gccagaaacg ccgatttgct ctgtgtggtg gtgcgtgatt
     1261 ttgccagtga tgacgtgttt catcctctgg gacgtgttga cgcacagggt gatcggcaga
     1321 acatcatgtc ggaattgatc ttggccgacc tgatgcttgt ggaaacaagg ttggagcgca
     1381 ttgccaagga caagttgaga caggccaaag cccaaaatct tttggttgaa gaaggggcct
     1441 tgcacaaatg caaggtggtg ctggatcagg aagcccctgt cagcacagtc acgtttgcca
     1501 aagaagaaca ggcgattctg aacaatttga acctgatcac acagaaatcc atcatgtggt
     1561 gcgtgaatgt ggccgaaggt cacgccaatc gcgcgcccgg caaaccagac gagtttattg
     1621 tgtcggcaaa aatagaaaag gaaattgttg gcattgaaaa tcccgaggaa cgccagatgt
     1681 tcatgcagga tttgggactc aaggactcgg gcttgaatcg attgaacgcc gccgcgtatc
     1741 atcagttggg tctcatgtcg ttttacacag tgggcccgga cgaagtccgt gcctggacca
     1801 tcaaacaagg ttccttggcg cccgaagcgg gaggcaagat tcattctgat attgagcgcg
     1861 gttttgtgag ggtcgaggtc atgaagtacg atgacctgat ggccgaaaaa agcgagcaga
     1921 aactcaaatc gctgggcaag gtgtacgtca aggggcgcga ctatgtgatc cacgacggtg
     1981 acatctgcaa ttttttgttc aatgtgtgag gcctctcgtt cttactcatc ggcattgtat
     2041 cttgccctaa aatagtattc aatcgcacta aagaatcgat tcatctcaga ctgtcttccc
     2101 gggctttggg actccagagc gcattctgca aaacttttag tgagtgggcg ataaaaatag
     2161 ggtggagaat atttgtctcc ttccttgtga taatcactgg ttaaaagaac cgcagggatc
     2221 agtggtgagt gaggaatgtc agtatttgcg gccaggtgaa aaagataaaa aacaaaagac
     2281 caatagctag tgcgatgatt gatgtgaagg cccacaggtt ttttgggtgt gcgacataaa
     2341 agtgggtaaa tttgattttg cccccccccg cctgttgtaa ggcgggggtg cccgtagggc
     2401 gggggtggta gaaaacatga ccaatttgaa agattttgag tgattgactt cctctttttt
     2461 ttggtccata atcttaatgt tcattaagca ttaagattat gg
//
LOCUS       DATDUN010000026         2797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_167562, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000026 DATDUN010000000
VERSION     DATDUN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167562"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VJC18_00345"
     CDS             complement(<1..699)
                     /locus_tag="VJC18_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HLD43854.1"
                     /translation="MKSFKRVLSWLIFFLFVSLPAFASEADLKIPDLKNGDPYMLLGG
                     ITPWNLLCWGAFIIIGTLGISLWLRHQIHKLPAHESMLDVAETIFQTCRTYLIQQGKF
                     LIVLFLFIAAAMAFYFLFLKGETITTLALVLLFSVVGMGGSYCVAWYGIRVNTYANAR
                     TAFASLAGNPWDVVNIPLRSGMSVGLFLISLELVMMVIILLCVPRTIVGYCFLGFAIG
                     ESLGASVLRIAGGIF"
     gene            complement(817..>2797)
                     /gene="lon"
                     /locus_tag="VJC18_00350"
     CDS             complement(817..>2797)
                     /gene="lon"
                     /locus_tag="VJC18_00350"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HLD43855.1"
                     /translation="DTIVAHLNLKIPDKQSILEITSPRERLEKLLSLMHGEIEILQVE
                     KKIRTRVKKQMEKTQKEYYLNEQMQAIQKELGERDDFNSEIQELDAKLKKKRMSKESS
                     EKVKKEIKKLKMMSPMSAEATVVRNYIDWVLSLPWADKTKDNIDVKRAHEILDEDHYG
                     LKKTKERILEYLSVQKLVGKVKGPILCLVGPPGVGKTSLGKSIARACERKFVRISLGG
                     VRDEAEIRGHRRTYIGAMPGKIIQSMKKADANNPVFLMDEIDKMNMDFRGDPASALLE
                     VLDPEQNFTFNDHYLDLDYDLSDVMFICTANSLNGIPPALMDRMETIRIAGYTEDEKL
                     NIAVRYLIKKQMEQNGLSEETICFSNSALRTIIRRYTREAGVRNLEREIANICRKVAR
                     TIAELKEGEPRVKTNISSKNAHNYLGIPKFHYGIAEEKDEVGITTGLAWTEVGGELLQ
                     IEVTIMPGKGKLTITGKLGEVMQESAQAAMSAVRSRAREMGLKKDFYEKIDIHIHVPE
                     GAIPKDGPSAGVTMATSITSALMRIPVYKDIAMTGEITLRGKVLPIGGLKEKLLAAHR
                     AQIKTVIIPKENEKDLKEVPKNILKALKIHAVSHIDQVLEKALAIKNIAVLYKNEKKG
                     ADLLDIYETDEVKPVEVVIAGTGEEKKVPDVIVPN"
BASE COUNT          659 a          736 c          683 g          719 t
ORIGIN      
        1 aaaaatacct ccggcgattc ttaataccga ggcccccagc gattcaccga tggcgaaacc
       61 cagaaagcaa taccccacga tggttcgcgg cacgcaaaga aggataatga ccatcatgac
      121 cagttccaat gagatcagaa acaagccgac gctcatgccc gaacgcaacg gaatattgac
      181 cacgtcccaa gggttgcctg ccaatgaggc aaaggccgtt cgcgcgttgg cgtaggtgtt
      241 gacccgaatg ccgtaccagg caacacaata agaccccccc atgcccacaa cagaaaagag
      301 caacaccaaa gccagcgtgg tgatcgtctc tcccttgagg aacaaaaagt agaaggccat
      361 ggccgcggca atgaacaaaa acaacacaat caaaaatttg ccttgttgga tcaggtaggt
      421 gcggcaagtt tgaaaaatgg tttctgccac atcgagcatg gattcgtgtg caggtaattt
      481 gtgaatttga tgacgcaacc aaaggctgat gcccaaagtg ccgatgatga taaaagcccc
      541 ccagcacaga agattccagg gtgtgatgcc ccccaacaac atgtaaggat caccattttt
      601 taaatccgga atcttcagat cggcctcgct ggcaaaagcg ggcagggaaa caaacaggaa
      661 aaaaatcaac caggataaaa cacgtttaaa cgacttcatg tccgtccttt cttaagataa
      721 ttgggttagg aataaacaac gaaaaagtca cacaagtgta taaggggata atgatcattt
      781 tgcaaatgta atgtgtcggg aaaaacgacc cgaatttcag ttcggcacaa tcacgtcagg
      841 aacctttttt tcttcgcccg tgcccgctat gacaacctca acgggcttca cctcatccgt
      901 ctcgtaaatg tccaaaaggt cggcgccctt tttctcgttc ttgtacaaga ccgcgatgtt
      961 cttgatggcc agggcttttt ccaaaacctg gtcgatgtgc gacacggcat gaatcttgag
     1021 cgccttgaga atgtttttgg gcacttcttt gaggtctttt tcgttttctt tggggatgat
     1081 caccgttttg atctgcgcgc ggtgagcggc cagaagtttt tccttgagtc cgccaatcgg
     1141 cagcaccttg cccctgagcg tgatctcacc ggtcatggcg atgtccttgt aaaccggaat
     1201 gcgcataagg gccgaggtga tcgacgtggc catagtcacg cccgccgacg gcccatcctt
     1261 gggaatggcc ccttcgggca cgtggatgtg aatatcgatt ttttcgtaaa aatctttttt
     1321 gagtcccatc tcacgcgcgc gtgaccgcac cgcactcatc gccgcctgag ccgactcctg
     1381 catcacctct cccagcttgc cggtgatggt gagcttgccc ttgcctggca tgatggtcac
     1441 ttcgatctgt aacaactcac cgcccacttc ggtccaggca agtcctgtgg tgataccaac
     1501 ctcgtccttt tcttcggcaa tcccgtagtg aaatttcgga atccccaaat aattatgggc
     1561 gttcttcgag ctgatattgg ttttgacacg aggttcacct tctttgagtt ccgcgatggt
     1621 ccgagccacc ttgcgacaga tgttggcgat ttcgcgttcc agattacgca caccggcctc
     1681 ccgggtataa cgccggataa tcgtcctgag agccgagttg gaaaaacaaa tggtctcctc
     1741 agacaaaccg ttctgttcca tctgcttttt gatgaggtaa cggacagcga tgttgagctt
     1801 ttcgtcctca gtgtaccccg cgatgcggat ggtctccatg cgatccatga gagccggggg
     1861 aatcccgttg aggctgtttg ccgtacagat aaacatgacg tcggacaaat cgtaatccag
     1921 atcgaggtag tggtcattaa aagtgaagtt ctgttcggga tcgagcacct ccaaaagggc
     1981 cgaagccgga tcaccgcgga agtccatgtt catcttgtcg atctcgtcca tgagaaaaac
     2041 cggattgttg gcatcggcct ttttcatgga ttgaatgatc ttgcccggca tcgcgccgat
     2101 gtaggtccgg cgatgaccgc gaatctcggc ctcatcacgc acgccgccca gtgagatgcg
     2161 gacgaacttg cgttcacagg ccctggcaat ggatttgccc agagaggttt tgcccactcc
     2221 cggagggcca acgagacaca ggatgggtcc cttgacctta cccaccaact tctgcactga
     2281 taaatattcc aggattcttt ccttggtttt tttgagtccg taatgatctt cgtccaaaat
     2341 ttcgtgggcc cgcttgacat cgatattgtc cttggttttg tcggcccaag gcagagacag
     2401 gacccaatcg atataattgc gcaccaccgt cgcttcggca gacatgggcg acatcatctt
     2461 gagcttcttg atttcttttt ttactttttc cgacgattcc ttgctcatcc ttttcttttt
     2521 gagtttggca tcgagttcct gaatctcgct gttgaaatcg tcgcgctcgc ccaattcctt
     2581 ttgaatggcc tgcatctgct cgttgaggta atattccttc tgcgttttct ccatctgctt
     2641 tttcacgcgc gtgcggatct tcttttccac ctgcaatatt tcgatttcgc cgtgcatgag
     2701 agaaagcagt ttttccagac gctctcgggg gctcgtgatt tcgaggatgc tttgtttgtc
     2761 gggaattttt aaattcaggt gggcgacaat ggtgtcg
//
LOCUS       DATDUN010000027         1319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_1734, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000027 DATDUN010000000
VERSION     DATDUN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VJC18_00355"
     CDS             complement(<1..325)
                     /locus_tag="VJC18_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43856.1"
                     /translation="MFLIIMAVIFVLVGIFVPMIIPKTTKEYDRRTNQTVEYSLSWVR
                     FLVRGVALVLATFFLFLTSYVTVDQDQVGHLKRIYLASDMPAGQIVALPGQKGPQAEI
                     LGPGFH"
     gene            complement(479..811)
                     /locus_tag="VJC18_00360"
     CDS             complement(479..811)
                     /locus_tag="VJC18_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43857.1"
                     /translation="MVEWQETVKRAYDLGQRLLGAPESKKRFVRLAFGTHTLQVQWEN
                     DNAMELHVSEPGVKETQTARLSLRPGKDQFVDGVVVTRQGQSEEALKALLDELLEKWR
                     LAECKFSN"
     gene            complement(834..>1319)
                     /locus_tag="VJC18_00365"
     CDS             complement(834..>1319)
                     /locus_tag="VJC18_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43858.1"
                     /translation="YSVLLDEWQKHRPKWEAARACLCDQLGKCIERERSEAEKYFTEA
                     VALTADGKDIVCCEEVVVIDGIKAIIYPAAAFAQNKILENQDKYRFSSDHVMNPAVLN
                     SWLRYIVINGRLETMGKGQFYSTLSSCVAKATDKDRFVTDIRYCPDPLPEGGREIKLD
                     V"
BASE COUNT          332 a          400 c          260 g          327 t
ORIGIN      
        1 agtggaatcc gggtccgaga atttcagcct gcggtccctt ttgcccaggc aacgccacaa
       61 tctgtccagc cggcatgtcg ctggccaggt aaatcctctt caggtgtcct acctgatcct
      121 gatctactgt gacgtagctc gtgagaaaca ggaaaaaagt tgccagaacc aacgccacac
      181 cgcgcacgag aaaccgaacc cagctcaaac tatactccac tgtttgattg gtcctccgat
      241 cgtactcctt ggtcgtcttg ggaatgatca tggggacaaa aatccccacc agaacgaaaa
      301 tcaccgccat gataatcaaa aacattccag cacctccatt ggttgttaac gaaccgcccg
      361 ttttaacggg acttgcaaac atatcaaaaa gcaatgtaaa tgtcaatagc agttttatat
      421 acaaaatttt ttgttcaatt ctacaaaaaa accgcccgat aatacgggcg gctgtcattc
      481 aattggaaaa cttgcattca gccagtcgcc acttttccag caactcatca agcagagcct
      541 tcaacgcttc ttccgactgt ccctgccttg tcaccacgac accgtccaca aactgatcct
      601 tgcccggacg caaactcaat cgcgccgtct gcgtttcctt cactccgggc tcggaaacat
      661 gcaattccat cgcgttgtca ttttcccact gcacctgcaa cgtgtgcgtt ccgaacgcca
      721 aacgaacaaa ccgctttttc gactccggcg cgccgagcaa tcgctgaccc aaatcatacg
      781 ctcgcttcac tgtttcttgc cattccacca tgttgccctc ctgcgttatg ttgttaaaca
      841 tccaacttga tttccctgcc accctccggc aacggatccg gacagtagcg aatgtcggtc
      901 acaaatcgat ccttgtctgt cgcttttgcc acacaagaag aaagcgtgct gtaaaattgt
      961 cccttgccca ttgtttccag tctcccgtta atcacaatgt atcggagcca agaatttaac
     1021 actgcagggt tcatcacatg atcggaagaa aaacgatact tatcctgatt ttccaaaatc
     1081 ttattctgtg cgaatgcggc cgcgggataa atgatcgcct ttatcccgtc tatcaccacc
     1141 acttcttcgc aacaaacaat gtctttgcca tccgccgtca gcgccacggc ctcggtgaaa
     1201 tacttttccg cctcggatcg ctctcgttcg atgcatttgc cgagctgatc acacaggcag
     1261 gcgcgcgccg cttcccactt tggccgatgc ttttgccact cgtcaagcag aacggaata
//
LOCUS       DATDUN010000028         1515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_458012, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000028 DATDUN010000000
VERSION     DATDUN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458012"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VJC18_00370"
     CDS             complement(<1..902)
                     /locus_tag="VJC18_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43859.1"
                     /translation="MSKTRIVLIVLLILSAVVYYFRTDLMYRYINYRTGGFSNMIPKA
                     STLKGTNLKEFAQSYHEAYRLKPDKRVIQSVAIIDQIISGQQPAPPQVHYQDGTWQIV
                     SHGVNVGTLPELSHFKDQMNFLTGWALALLQKTPLNGNGAEGADLVFGELERKSPKTI
                     FAYLGRLNQRWLTGEKRVGDVANAARAYLWLVLYGEDALEVSDNISRALGLIALSEAL
                     GTDMTREKALVADLFQFGAFALELTKKLDADDTVALYIKRVDEQLELKANRPGAEREA
                     HFFWLRRLVQNYDLTRWQEFSKKFV"
     gene            complement(895..>1515)
                     /locus_tag="VJC18_00375"
     CDS             complement(895..>1515)
                     /locus_tag="VJC18_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43860.1"
                     /translation="GHPAYKNMGARAGYTLMTALFVGLGGMLGYVAWVVDAIPSAAVA
                     PILLFVGLEITVQAFGVCEKKHFSAIYLALLPCVGELVRIVVATVLFAPGVVGHFPDA
                     GSDAAHLWQVSNILGHGFIITSMLWAAVVVKLVDKQIWGASVFLVIMAFLTVFGFIHS
                     VTPDGGIYLPWAMENAKLPMTMAGTYLFLAVLFVGVSGKLKEKSHV"
BASE COUNT          409 a          449 c          324 g          333 t
ORIGIN      
        1 acaaattttt tggaaaattc ctgccatcga gtcaaatcgt agttctggac cagacgtctc
       61 agccaaaaaa aatgagcctc tctttctgcc cccggtctat ttgccttgag ttccaactgc
      121 tcatcaacgc gtttgatgta aagggcgacc gtatcgtccg catcaagttt tttggtcaac
      181 tccagagcaa aggcaccaaa ttgaaacaaa tccgccacca gggccttctc acgcgtcatg
      241 tctgttccca acgcctcgga aagagcaatc aggcccaatg cgcgggatat gttatccgaa
      301 acctccagcg catcctcacc gtacaaaacc agccacaaat aggcccgagc cgcattggcc
      361 acatctccca ctcgtttttc accggtgagc caccgctgat tcagtcgtcc caaataagca
      421 aatattgttt tgggtgattt tctttccaac tcgccaaaca ccaggtccgc tccctctgcg
      481 ccgttcccat taagtggtgt tttctgcaac aaggccaaag cccaacctgt taaaaaattc
      541 atctgatcct taaaatgcga aagttcaggc aatgtcccca cgttgacacc gtgggacaca
      601 atctgccaag tcccatcctg ataatgaacc tgtgggggcg ctggttgttg cccagaaata
      661 atctgatcga tgatggcaac agactggatc acccttttgt ctggtttgag gcgataggcc
      721 tcgtgatagc tttgcgcaaa ttccttgagg ttggtaccct tgagtgtgga cgccttgggg
      781 atcatgttgc tgaatccccc cgtgcggtag ttgatgtagc gatacatcag atcggtacga
      841 aaataataga ccacagcgct caagatcaat aatacgatca aaacaattct tgttttagac
      901 atgtgacttc tcctttaatt tccccgacac cccaacaaac agcacagcca gaaacaaata
      961 cgtccccgcc atggtcatgg gtagttttgc gttctccatc gcccaaggca gatagatacc
     1021 tccatcgggt gtcacggaat gaatgaatcc aaagacagta agaaacgcca tgatcaccag
     1081 aaacaccgag gccccccaga tttgtttatc gaccagtttg accaccaccg ccgcccacaa
     1141 catggacgtg atgatgaacc cgtgacccaa tatgttcgac acctgccaca gatgcgccgc
     1201 gtctgaaccg gcatcgggaa aatgccccac cactccgggg gcaaacaaga ccgtggccac
     1261 aacgatccgc accagttcac ccacgcaggg cagaagtgcc agatagattg cggaaaaatg
     1321 ttttttctcg caaaccccaa aggcctgtac tgtgatctcc aacccaacaa aaagaagaat
     1381 cggcgcgact gcggccgaag ggatcgcatc caccacccag gccacataac ccagcatccc
     1441 acccagacca acaaataacg cagtcatcaa cgtgtaccct gccctcgccc ccatgttctt
     1501 gtatgcgggg tgacc
//
LOCUS       DATDUN010000029         1495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_250681, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000029 DATDUN010000000
VERSION     DATDUN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250681"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..234)
                     /locus_tag="VJC18_00380"
     CDS             complement(25..234)
                     /locus_tag="VJC18_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HLD43861.1"
                     /translation="MKKEYDFSSSKKNPYAKYLKKQITIRLDDSVISYFKKLARNVGI
                     SYQNLINLFLADCAKHGKKPSVKWS"
     gene            complement(218..523)
                     /locus_tag="VJC18_00385"
     CDS             complement(218..523)
                     /locus_tag="VJC18_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011220382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HLD43862.1"
                     /translation="MIMIRFEWDIQKNSINERKHGLNFEEAQEVFEDPFARLIADQDH
                     SLGEDRFVLMGMSHRQNTIVVCHCYRRRNKVIRIISARRATPFERKQYEEFRYEKGI"
     gene            complement(568..>1495)
                     /locus_tag="VJC18_00390"
     CDS             complement(568..>1495)
                     /locus_tag="VJC18_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43863.1"
                     /translation="CVVAGDAGCLPTQSWRALGDDGALDVNETEGPWWVEGAPLYGVN
                     DYLFDIPQNWADEYVGGRRLVAGRFRDGGQGSQGPTMFAFGPWLEGNPPAADSTISSV
                     ELLYYSTYPETTDWLEDYQHPDEWAGGAWLTTADKAAVIFVGTKSLGDHYWYGWQHCP
                     DDLIPCVEPEAVGGPGCYTAEGDDCGLAAEYYCDCDDISCDEDCYGGRGWWTEEFETQ
                     IAFYDPDDFAAVAAGVTEPSSPQPYTTMNIDDAMFLTTPAGALDEYGEGVQRRYRVGA
                     VAYDRTNNLLYVTELFGDVENEQPLVHVWGVE"
BASE COUNT          373 a          447 c          311 g          364 t
ORIGIN      
        1 cgaagctttg cggggactgg caccttatga ccacttcact gacggttttt ttccgtgctt
       61 ggcgcaatct gccaaaaaaa gattgatcag attctggtag ctgatgccaa cattcctggc
      121 gagtttctta aaataactga tcacagaatc atccagccgg atggtgattt gttttttcag
      181 gtatttggca taaggatttt tttttgatga agaaaagtca tattcctttt tcataacgaa
      241 attcctcata ctgtttccgc tcgaaaggcg tggcccttct ggcactaatg atcctgatca
      301 ccttgtttct ccggcgataa caatggcaaa caacaatggt attttgccga tgactcatcc
      361 ccattaacac aaaacgatct tcccccagtg aatgatcctg atctgctatc aatctggcaa
      421 atgggtcttc aaagacctcc tgagcctctt cgaaattaag gccgtgtttt ctttcgttga
      481 tgctgttttt ttgaatatcc cattcaaatc gtatcataat tatataataa ttacaaatta
      541 attatatttt gcccctcttc acgcacccta ctccaccccc cacacatgca cgaggggttg
      601 ctcgttctcg acatcgccaa aaagttctgt gacatacaac agattgttcg tgcgatcgta
      661 cgccaccgct cccacgcggt aacgcctttg caccccttca ccgtattcat ccagcgcccc
      721 cgcgggcgtg gtcagaaaca tcgcgtcgtc gatgttcatg gtcgtgtagg gctggggtga
      781 actgggttca gtcacaccag cagccacagc ggcaaaatca tccggatcat aaaaggctat
      841 ctgcgtctcg aattcttccg tccaccaacc acgaccacca tagcaatctt cgtcgcagga
      901 gatgtcatcg caatcacaat aatactcagc ggccaatcca caatcatctc cctccgccgt
      961 ataacaaccc ggtcccccca ctgcctccgg ctccacacag ggaatcagat catcgggaca
     1021 atgttgccag ccataccagt aatgatcccc caaacttttt gtccccacaa aaatcacagc
     1081 cgccttgtct gccgtagtga gccacgcccc acccgcccat tcatcggggt gctgataatc
     1141 ctccagccag tccgttgttt cgggataagt tgaataatac aaaagttcca ccgaactgat
     1201 cgtagaatcg gccgccggag gattaccctc gagccaaggg ccaaaggcaa acatggttgg
     1261 cccctgactg ccctgccccc catcacgaaa acggccggcc acgagcctcc ggccacccac
     1321 atattcatcg gcccagtttt gggggatgtc aaagaggtaa tcgttcacac cgtaaagagg
     1381 ggcgccctcg acccaccaag ggccttcggt ttcgttaaca tcaagtgcgc catcgtcccc
     1441 cagcgcacgc cacgactgcg tggggagaca gccggcatca ccagcgacaa cacaa
//
LOCUS       DATDUN010000030         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_125961, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000030 DATDUN010000000
VERSION     DATDUN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125961"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VJC18_00395"
     CDS             complement(<1..461)
                     /locus_tag="VJC18_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43864.1"
                     /translation="MTRLPGMATTIPLTSTITPTTNLTPLIDLSQTTTDVERFGDLYT
                     PLARQVPLVPYRMTFKQAYYRHYLPVRLDERVMRSYQDMNPETRASLFPHHSTEWLPP
                     QDCADEVVVAQRLVAIARLDPEHTQLLLFQGGLWLIAGPTTTANTLLDLMA"
     gene            complement(578..862)
                     /locus_tag="VJC18_00400"
     CDS             complement(578..862)
                     /locus_tag="VJC18_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HLD43865.1"
                     /translation="MKNKLTAEEKDLLDSYERGEWKKAHPKNKLPVQTKIYAVYARHT
                     IAKDSRVNIRVSSKDIEGLQKLAIQEGIPYQTLIASILHNFVSGRLARKA"
     gene            complement(859..1134)
                     /locus_tag="VJC18_00405"
     CDS             complement(859..1134)
                     /locus_tag="VJC18_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HLD43866.1"
                     /translation="MKTIVWDNEKNLKLKAERGVSFEDVLFHIMRGDILDLLGHPGKK
                     HKHQQLMVVRINDYAYLVPFVETENEILLKTIIPSRKATKNYLSEDE"
     gene            1541..>2594
                     /locus_tag="VJC18_00410"
     CDS             1541..>2594
                     /locus_tag="VJC18_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD43867.1"
                     /translation="MFNRTIKEEIISFISDGYNNKPVLVLSGARQTGKTTILKNILQE
                     KPHLFLDLETDRSYADRINQFKDFKEFDAWLRDEFSFDPSRQILCIDEAQCSQGLGCF
                     VGAMKEKWPNSNVIITGSLISELYDSATRRPVGRETFLNIWPLTFREFLLALGYDNLV
                     KVMDEFIPGDEISQANHNRFLDAYDVYLKVGGLPEVVNYYLQKRDYKKRRNDIFQSYQ
                     DDFARYFNHEDISLFMRCLTAVAQNAGSPSKDSQAVRPNVYGYRKVPSIYGRLEKWKL
                     IIKCDQIGIRPEQNKYHPKRYLYDVGVLCDLRLRGIETVGVRDLENEHLRAPLGGVIE
                     NALALSLRSQFDEEIFG"
BASE COUNT          676 a          563 c          605 g          750 t
ORIGIN      
        1 tcagccatga ggtccaacaa agtgtttgct gtcgtggttg gcccggcgat caaccataac
       61 cccccttgga atagcaataa ctgtgtgtgt tcgggatcga gccttgctat ggcaaccaat
      121 ctttgcgcca cgacgacttc atcggcacag tcttgtggtg gtagccattc cgtgctgtga
      181 tggggaaaga gcgatgctcg cgtttctggg ttcatgtctt gatacgatcg catcacacgc
      241 tcatccaacc gcacaggcag gtaatgccga taatatgcct gtttgaaagt cattcggtac
      301 gggaccaggg ggacctgtct ggccagtggc gtgtacaaat caccaaatcg ttcaacgtca
      361 gttgttgttt gggacaaatc aatgagaggt gtcaaattcg ttgtgggcgt gatggtactg
      421 gtgaggggaa tcgttgttgc catgcctggg agccgagtca tatgtttctg ctaagcgcct
      481 ttcaatgatc tgtcaaattt atcctaggga caagcgcttt ggtcttgacg agcgggccat
      541 ccatatgagg ctcttatggt gccgaatata gctggattca tgcttttctt gccaatcggc
      601 cagaaacaaa attgtgcaaa atactcgcaa tcaacgtttg atacggaatc ccctcttgaa
      661 tggccagttt ttggagcccc tcgatatcct tggatgacac cctgatgttg acgcgactgt
      721 cttttgcaat ggtatgacgc gcatagactg cataaatctt ggtttgcaca ggcagcttat
      781 ttttcggatg ggctttcttc cattcgccac gttcgtacga atcaagcaaa tccttttctt
      841 ctgcggtgag tttgtttttc attcgtcctc cgaaagataa ttcttagtcg ccttacggct
      901 cggaataatg gtttttaaca atatttcgtt ttctgtctct acaaacggca caaggtacgc
      961 gtaatcgttg atgcgcacca ccattaattg ctggtgtttg tgctttttgc cgggatgacc
     1021 aagcaaatcc aaaatatcac cacgcatgat gtgaaaaaga acatcttcaa aagaaacacc
     1081 acgttcagct ttcaatttga gatttttttc gttgtcccaa acaatcgttt tcatctgatg
     1141 ccaataatag cacaatgtgt gccttttggc aacaattcaa tacccctaca cgttgtttcc
     1201 acctgcgcca aggcttccac ctacgctaaa gcttcggtgg acaggtcggt ggacaggtcg
     1261 gtggacaggt cggggcacaa gttgacttgg tttaggcgca cagattcaga catgaaatca
     1321 ttcatgtctt ccgcaatgca cttcaccgtg gtttgtgccc gtaaggaaat gcccttgggg
     1381 taccacggaa tcttgtgtcc agggcttgcc ttgggggtac ataccggcga ttcgtgccag
     1441 tcccctaaat tcgcaaagga ttggtggtaa aaactaaaaa tcggtaaatt attgcatgaa
     1501 ttgtcttgat ttacggtaaa ttattgctat gatttgcaat atgtttaaca gaacgattaa
     1561 agaagaaata atatctttta tatcagatgg ttacaacaac aaaccagttc tggttctttc
     1621 tggggcgcgt caaacgggca aaaccacaat cttaaaaaac attcttcaag aaaaaccaca
     1681 tctgtttttg gacctggaaa ccgataggtc ctatgctgat cgcataaatc aattcaaaga
     1741 ttttaaggaa tttgacgcgt ggctcagaga tgaattttca tttgatccat cacggcaaat
     1801 tctctgtatc gatgaggctc aatgcagtca aggtttgggc tgttttgttg gcgccatgaa
     1861 agagaaatgg ccaaacagca atgtcatcat tacaggctcc ttgatcagcg agctttatga
     1921 ctccgcgacg cgtcgtcctg tgggacgcga aacattttta aatatctggc ctctcacatt
     1981 cagggaattt ctgctggctt tgggttacga taatcttgtt aaagtcatgg atgaatttat
     2041 tccgggtgat gaaatttcac aagccaatca caaccgcttt ttggatgcct acgatgtcta
     2101 tctaaaagtt gggggtcttc cagaggttgt gaattattat ctacagaaaa gggattataa
     2161 aaaacgtcgt aatgacattt ttcaatcgta tcaggatgat tttgcacgtt acttcaatca
     2221 cgaagacatc tctcttttta tgcgttgcct cacagccgta gcccaaaacg ctggatcacc
     2281 gagcaaggac tcgcaggctg tgcgtcccaa tgtttatggt tatcgcaagg tgcctagtat
     2341 atacggaaga ttggaaaaat ggaaactcat tatcaaatgt gatcaaattg gaatacgacc
     2401 tgaacagaat aaatatcatc ccaagcggta tttgtacgat gttggtgttc tttgtgacct
     2461 gcgtctgcgg ggaatcgaaa ctgtgggtgt tcgcgatctt gaaaacgaac acctccgcgc
     2521 tccgctgggt ggtgttatcg aaaatgcctt ggcgctgagt ttaagatccc aattcgacga
     2581 agaaatattt ggaa
//
LOCUS       DATDUN010000031         1739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_43132, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000031 DATDUN010000000
VERSION     DATDUN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43132"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VJC18_00415"
     CDS             <1..574
                     /locus_tag="VJC18_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HLD43868.1"
                     /translation="MGPHTEEGLRPLICFVALLFCFIVFVPTSRAQTLAAPNTNWFLG
                     LGGHAGMEVFEITSFGGGVSLPFGYHINKNISVYGMTDVFITNDSGINYVFASFMPMA
                     TYRFYKWFSVYGGAGYALMHPSRAGRIWDFRPSTTSYYHGLRGEFGFGYNPQVSKRVV
                     VAPQIGVQYTRIANDDLLIPLVRINIILNL"
     gene            complement(582..962)
                     /locus_tag="VJC18_00420"
     CDS             complement(582..962)
                     /locus_tag="VJC18_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD43869.1"
                     /translation="MPATKRILIVDDERDLVETLTYRLEALGYEVLTAYDGQEGLDRV
                     REEKPDLILLDVMMPKLDGYHVCRLIKFDDDLKGIPIIMLTARGQPQDRQTGRDSGAD
                     DYLTKPFESAVLLEKIQQLLKPKA"
     gene            complement(994..1356)
                     /locus_tag="VJC18_00425"
     CDS             complement(994..1356)
                     /locus_tag="VJC18_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD43870.1"
                     /translation="MAKIMICDDAVFMRETIREIVETAGHEVVAEAETGVDAQKQYRQ
                     HKPDLVTMDILMKDSGVEAIKAIVKTDLRAKIIVVSILSDQEGEIVEAIRAGALGIVT
                     KPVRKEVLLSEITRVMAK"
     gene            complement(1426..1689)
                     /locus_tag="VJC18_00430"
     CDS             complement(1426..1689)
                     /locus_tag="VJC18_00430"
                     /inference="COORDINATES: protein motif:HMM:NF022179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HLD43871.1"
                     /translation="MHIQNYMEEMVNREIDSVLSLSNSGCSCEQCRTDIAAWVLNRMP
                     AIYVVSDNGRTYSKLKEFDYQLKAELTVRIVEASNIVAKNPRH"
BASE COUNT          404 a          445 c          368 g          522 t
ORIGIN      
        1 tatggggcct catacggagg aaggtttgag accccttatt tgttttgtcg ctcttctttt
       61 ttgtttcatt gtttttgttc ccacatcacg cgcacaaacg ctggccgcgc ccaacaccaa
      121 ctggtttttg ggtcttggcg gacacgcggg gatggaggtt tttgagataa caagctttgg
      181 tggcggcgtc agccttcctt ttgggtatca catcaataaa aacatttctg tttatggcat
      241 gacggacgtt tttataacaa acgattccgg catcaattat gtatttgcct ctttcatgcc
      301 catggcgacc taccgttttt ataaatggtt ttcggtttat gggggagccg gttatgccct
      361 gatgcaccca tcgcgcgcgg ggcgtatttg ggatttccgc cccagcacaa ccagttacta
      421 tcacggtctg cgcggtgaat ttggttttgg atacaaccca caggtttcaa agagggtggt
      481 cgttgcccct caaatcggtg tgcaatacac caggatcgct aatgacgacc tgctcatccc
      541 gcttgtgcgc atcaatatta ttttgaattt gtagtctccc cctatgcctt gggcttcagg
      601 agttgctgga ttttttcgag caaaacggca ctctcaaatg gtttggtaag gtaatcatcc
      661 gcccctgaat ccctgcccgt ctgacgatcc tgcggctgtc ccctggccgt gagcatgatg
      721 atggggatcc ccttgagatc atcatcaaac ttgatcagtc gacacacatg gtacccatcg
      781 agcttgggca tcatgacatc cagcagaatc aaatcgggtt tttcttcgcg cacccgatcc
      841 agaccttctt ggccatcata ggcagtcaaa acctcatacc ccaacgcttc cagccgatac
      901 gtcaatgttt ccaccaggtc tctttcgtca tcaacaatga gtattctttt tgttgccggc
      961 ataatgtttc ctccaagatc tgccatgata aaatcacttg gccatcacgc gtgtaatttc
     1021 agaaagcaaa acctctttgc gcacgggttt tgtaacaatt cccaatgccc cagcgcgaat
     1081 cgcctcgaca atttcacctt cttgatcaga cagaatactc acaacaataa ttttcgcccg
     1141 aagatcagtt ttgacaatgg ccttgattgc ttccacgcca gaatccttca tgagaatatc
     1201 catggtcacc agatcaggct tgtgttgccg gtattgcttc tgggcatcta ctcccgtttc
     1261 ggcctcagca acaacctcgt gccctgccgt ctcaactatt tcccgaatcg tttctctcat
     1321 gaagaccgca tcatcacaaa tcattatttt tgccataaca acctctccta gttattgttt
     1381 ccttattaag gaccgggggt caattctcaa cctattccag acttgttaat gcctggggtt
     1441 tttggccaca atatttgatg cctccacaat acgcaccgtg agttcggctt taagctgata
     1501 gtcgaattct tttaacttgg aataggtgcg accattatct gagacgacat aaatcgcggg
     1561 cattctgttg agaacccaag cggcaatgtc ggtgcgacat tgctcacaag agcaaccaga
     1621 gttggaaaga ctcaagaccg agtcaatttc tcgattgacc atttcttcca tataattttg
     1681 tatatgcata actttttgat attaaatgtg gttttgtttt ttgtcaaacg taatgcgtt
//
LOCUS       DATDUN010000032         1066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_126055, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000032 DATDUN010000000
VERSION     DATDUN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126055"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VJC18_00435"
     CDS             complement(<1..1056)
                     /locus_tag="VJC18_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43872.1"
                     /translation="MVAQGPVPNYRYLSPSISSPSDKNSIPNWAIDVSATSPPPILNM
                     DDYWNYARDFVAVYKAKAPHVQPRIYDFFDEVDLNTEAGNAGGSLTTHVPYWSTDDVV
                     DWAQQGKEFIEEHDPGAIVIGPQHTGSLRMPDLNFLEEVLEAGLLDYIDGYSLHAYHT
                     PPAEDSHVLERLREIKALLRQYNEGEDFPIHQTETGYCLNYPNRDALRDHASWHVRLA
                     TILKGEGVVRHYVFYEIDVLNLSNAYGLNFNLDPELRHGDWPRAPKPAVPALAVAAKM
                     LEGYYPVEDFIDQAQDRWGYRLSDGVSSVVVVWDPHGTQTMDVSVGGATTVTEVDIMG
                     HVTQHTASGGSVSVAIGP"
BASE COUNT          247 a          311 c          259 g          249 t
ORIGIN      
        1 cgggccaatg gccacagaca cagagccgcc gcttgctgtg tgctgggtca cgtgccccat
       61 aatgtctacc tcggtcactg tcgttgcgcc gcccacagac acgtccatgg tctgggtccc
      121 atggggatcc cacaccacca caacagaact gactccgtcg gaaagtcggt acccccagcg
      181 gtcttgcgcc tgatctataa aatcctccac aggataatac ccttcgagca tctttgccgc
      241 caccgccaat gcgggcacag cgggcttggg cgccctgggc caatcaccgt gcctcagttc
      301 tgggtcaaga ttgaaattga gcccataggc gttactgagg ttcaaaacat caatttcata
      361 aaacacataa tgccgtacca caccttcacc cttcaggatc gtcgccagcc tcacatgcca
      421 actggcatga tcccgcaacg catcacggtt tggatagttc agacaataac ccgtctctgt
      481 ctgatgaatg ggaaagtcct ctccctcatt gtattgtctg agcagggcct tgatctcgcg
      541 cagtctttcc aacacatggc tgtcctccgc gggtggcgtg tgatacgcgt gcagggaata
      601 accatctatg taatcgagca aacctgcttc aagaacttct tccaaaaaat tcaaatcagg
      661 cattctgaga gaaccggtgt gttgaggccc gatgacaata gcgcccggat catgttcttc
      721 gatgaattcc ttgccctgct gggcccaatc aaccacgtcg tctgtcgacc aatacggcac
      781 gtgggtggtc agtgagccac ctgcgtttcc cgcctcagtg ttcaaatcaa cctcgtcgaa
      841 aaaatcatag attctgggtt gcacatgcgg tgctttggcc ttgtacacag ccacaaagtc
      901 ccgcgcataa ttccaataat cgtccatgtt gagtataggc ggtggcgaag ttgcgctcac
      961 atcgatggcc caattcggaa tggaattttt atctgacggt gatgagattg atggcgacag
     1021 atacctgtaa tttggcacgg gaccttgcgc caccatggtc tgcgcc
//
LOCUS       DATDUN010000033         1309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_334728, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000033 DATDUN010000000
VERSION     DATDUN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334728"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VJC18_00440"
     CDS             <1..702
                     /locus_tag="VJC18_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43873.1"
                     /translation="KRVYHGAFHDGILCHLEVPSCDLTRNLPYFESYGFDKIKQLKEL
                     SLKKQVPLIDLLIKSDCLGKQDLALGYSSELRRLFLSITEESLASFRFVPARLKHWRR
                     VPALFDHQFIFQNEKLRAHNFVARCIHLNTHQKDSYLYMMARGSHDYKFCHEKTFREV
                     QRLIQILGHQFDQIVIDAPGLSGGQDGEIFSRLSDGVVLVVDGAEDSSAVIQTMMRRL
                     QDANVNVLGTVLNGA"
     gene            835..>1309
                     /locus_tag="VJC18_00445"
     CDS             835..>1309
                     /locus_tag="VJC18_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLD43874.1"
                     /translation="MKVYKTENLRNVCLLGDKGVGKTTIAEAALFTAKLTTRLGKVDE
                     GNTVVDYDQQEIDRKQTLTSKMLAFEWEGCKLNLLDTPGYADFIGESQAALWVADSAC
                     IVIDAGRHMTIGTKKYKDLVSALGKPCSFFINRLDSTRADWDTSLSQIKNATNNRA"
BASE COUNT          323 a          293 c          341 g          352 t
ORIGIN      
        1 aaacgcgtgt accacggggc ttttcacgat gggattttgt gtcatctcga ggtcccttcc
       61 tgcgatctga cgagaaactt gccttatttt gagtcgtatg ggttcgataa aatcaagcaa
      121 ttgaaggaac tttccctcaa gaaacaggtc cctctcatcg accttttgat caaatccgat
      181 tgtttgggta aacaggatct ggctctgggg tactcgagtg agctgaggcg cttgtttttg
      241 agtataacag aggagagcct ggcttcgttt cgtttcgtgc ctgcacgatt gaagcattgg
      301 cgacgtgttc ccgcgctttt cgatcatcaa tttatttttc aaaacgaaaa actcagggcc
      361 cacaattttg tggcccgttg tatccatttg aatacccacc aaaaagacag ctatctttac
      421 atgatggctc gcggttcgca tgactacaaa ttttgtcacg agaaaacctt tcgagaggtg
      481 cagcgactca tccagatatt aggacatcaa ttcgatcaaa ttgtgatcga tgcgcctggc
      541 ctctctggcg gacaggatgg cgagattttc agccgcctgt cagatggggt tgtgttggtg
      601 gtggatggtg ccgaggattc ttctgctgtg atccaaacca tgatgcggcg tttacaggat
      661 gccaatgtga acgtgttggg cacagtcctc aatggggctt gagggcgatc actttattat
      721 tgacctgatt atataaactg ttagaggcca tcttccctta accgcagagc aggtcgcaag
      781 gctttgttca tgggtccttg tgccacgcta cttgccaaat caatttgaga acttatgaaa
      841 gtatataaaa ccgagaattt gcgtaatgtc tgtttattgg gagacaaggg tgttggcaaa
      901 acgaccatag ccgaagccgc cctgtttacc gcgaagctta cgacccgttt gggaaaggtt
      961 gatgaaggga ataccgtggt tgattatgat caacaggaga ttgatcgcaa acagaccttg
     1021 acctccaaaa tgctggcgtt tgagtgggaa gggtgcaaat tgaatctcct cgataccccg
     1081 gggtacgccg atttcattgg agagtctcaa gcggccctgt gggttgccga ctcagcctgc
     1141 atcgtgatcg atgcggggcg tcacatgacc attggcacta aaaaatacaa agaccttgtg
     1201 agtgccttgg gcaagccctg cagttttttc atcaatcgct tggatagcac tcgggcggat
     1261 tgggacacaa gcttgtcaca gatcaaaaat gcgaccaata accgggccg
//
LOCUS       DATDUN010000034         1926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_167860, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000034 DATDUN010000000
VERSION     DATDUN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167860"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1448
                     /locus_tag="VJC18_00450"
     CDS             90..1448
                     /locus_tag="VJC18_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HLD43875.1"
                     /translation="MICFVISFALSLICPVGGHAQTDEGTSFSLDQALTFPEKIIRLD
                     LSNQHIVILDPKMGALQRLSHLNLDHNLIEQLPDEIGQLTSLQSLFVNNNKLTRLSTQ
                     IAKLPRLLWLQAGHNNIIHLPKTLGSLRGLELLHLQDNQISELPEEVGQLTSLLEIDL
                     GHNLLRLLPKTFENLLRLKTLTLSGNKLEEFGFDIALLTRLEWLQMNDNLFKELPQGI
                     TALHHLLEIEFADNKLEEFPTPLLALTQLEMINLSGNQIKTIPDDIARMENLRVLNLQ
                     DNLITKLPASIAKLTKLQELKLEGNHFSDEEIKKITSLFPENMVKWQRVYTSLASALQ
                     AGAQKVDKLFLNHHGLTSFPADILELFHLQELWLGNNAIVKVPPQIHRLNQLHKLDLS
                     HNKLRDLPAELVELTQLKWLNLVGNEFSDKEKQKIRALFTETEVIFEVLPDNPNKKID
                     DI"
     gene            1519..>1926
                     /locus_tag="VJC18_00455"
     CDS             1519..>1926
                     /locus_tag="VJC18_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43876.1"
                     /translation="MIKRKVFFMLFILLSVFVFDARAAYQSRFDAVNFEPAVDDSNYF
                     TVYGSRTLTAWQGHAGFYFDYANRPLEFRATAGAVGRQSVLDHTIIFDALGSIGITDW
                     FEVGVNMPVALYNWYYTDDVNAAADFGSSMGDML"
BASE COUNT          501 a          436 c          478 g          511 t
ORIGIN      
        1 gtcatgggaa aaagcaagga tgatagcgaa tacgaaaaac acatgacacg caggagtgga
       61 tattttatgt gagggacatg aatgttcgca tgatctgttt tgtcatttcc ttcgccctga
      121 gtctgatctg tcctgtgggg gggcatgccc agacggatga ggggacgtca ttttccctgg
      181 atcaggcgct gacctttccc gaaaaaatca tcagattgga tttgagcaat cagcatattg
      241 tcatcctcga tcccaaaatg ggggcgttac agcgattgtc gcatctcaac ctcgatcata
      301 atctgatcga acagttgccc gatgaaatcg gtcaactcac ttccttgcag agcctgtttg
      361 tcaacaacaa caagctcacg cgtctttcca cacagatcgc aaaattaccg cggctgttgt
      421 ggcttcaggc ggggcacaac aacatcattc acctccccaa aaccttgggg agtctgagag
      481 ggctggagtt attgcatttg caggacaatc agatttccga gctgcccgag gaggtggggc
      541 aattgacatc gctccttgag attgatcttg gtcataacct gctgaggtta ttgcccaaga
      601 catttgagaa tcttttgcgc ctcaagaccc tcacactgag cgggaacaaa ttggaagagt
      661 ttggatttga tatcgctttg ctgacccggt tggagtggtt gcagatgaat gataacctct
      721 tcaaggaatt gcctcagggc attacggcac tgcatcatct gttagagatt gagtttgccg
      781 ataataaact cgaagagttt cccacgccct tgttggcact cacgcaactg gagatgatca
      841 atctgagcgg aaaccaaatc aaaaccattc ccgatgacat agcccggatg gagaatcttc
      901 gcgtactgaa tttgcaggac aacctcatca caaaacttcc tgccagcatc gctaaactga
      961 caaaacttca ggaactgaaa ctcgagggga atcatttttc cgatgaggag atcaaaaaga
     1021 tcaccagcct gtttcccgag aatatggtga agtggcagcg ggtgtacacc tcgttggctt
     1081 cggcattgca ggcaggggcg caaaaggttg ataaactttt tttgaaccac catggactca
     1141 cgagcttccc agccgacatc ctcgagcttt ttcatctgca ggaattgtgg ctgggtaaca
     1201 atgccatcgt caaagtgcca ccacagattc atcgattaaa ccagttacat aaactcgatc
     1261 tgagccacaa caagctcagg gacctgcctg ccgagttggt agaactcacg caactcaaat
     1321 ggctcaatct ggtgggcaat gagttttccg acaaggaaaa acaaaaaatt cgagccctgt
     1381 tcaccgaaac agaggtgatc tttgaggttt tgccagataa tcccaataaa aagattgacg
     1441 acatctgata ttttggtaat tgttccgcca agttgaattg tgtttgaggt ggttgatctc
     1501 attaataaag gagagagtat gatcaaacgg aaagtttttt tcatgttgtt tattttgtta
     1561 tcggtgtttg tttttgatgc gcgggcggcc tatcaatcac gctttgatgc cgtcaacttt
     1621 gaacctgccg ttgatgacag caattatttt accgtttacg ggtcgcgcac actcacagcc
     1681 tggcaagggc atgccggttt ttattttgat tatgccaacc gaccattgga gttcagggcc
     1741 accgcggggg ctgtcggtag acagtctgtt ttggatcaca cgatcatttt cgatgcgttg
     1801 ggcagtatcg ggattaccga ttggtttgag gttggtgtga acatgcccgt ggctctgtac
     1861 aattggtatt acactgacga tgtcaacgca gctgccgatt ttggttcgag catgggcgac
     1921 atgttg
//
LOCUS       DATDUN010000035         1196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_43213, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000035 DATDUN010000000
VERSION     DATDUN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43213"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1196)
                     /locus_tag="VJC18_00460"
     CDS             complement(<1..>1196)
                     /locus_tag="VJC18_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HLD43877.1"
                     /translation="QFYFNISRRLLNHLASKGREEARLLINEIGGSLPVSPDTLYTAE
                     IDTNRPLKKRIYKIDPLMAEKIFALAESILGDEQIFSLVQKWVKENKLSFLVKVLDDN
                     RSSLGDIIDAIVRFKNSQLSEQELPFSTMNAINVSLIRRFFSRRLDFINIAKTHITID
                     DFYDLTKRLIFTPASDGKVGGKGAGLFIAMNIIKEEQKNFPELAGIKAPKTWYIASDG
                     VIDFINHNHLQELREYKYKELSQIRADYENIVQLFKNSSFSAEMIKGLSLALDDFGTN
                     PLVIRSSSLLEDSFDAAFSGKYKSLFIANQGTKDERLEALIDAIAEVYASMFHHDPIE
                     YRAERGLLDYQEEMGILIQEVVGTKVGHYLLPSFGGVAFSRNEFRWSPRIKREDNLVR
                     IVPGLG"
BASE COUNT          309 a          279 c          298 g          310 t
ORIGIN      
        1 tgcccaggcc cggcacaatg cgcaccagat tatcttcgcg tttgatgcgt ggtgaccagc
       61 gaaactcgtt gcggctaaag gccacaccac caaagctggg caaaagataa tgacccactt
      121 ttgtgcccac cacttcctga atgaggatgc ccatttcttc ctgataatcc aaaaggccgc
      181 gttcggcgcg atactcaatg ggatcgtgat ggaacatcga ggcgtagacc tcggcaatgg
      241 catcaatgag ggcttccagc cgctcatcct tggtgccctg attagcaatg aagaggcttt
      301 tgtatttacc ggaaaaggcc gcatcaaaac tgtcttccaa aagacttgaa gaacgtatca
      361 ccagtgggtt ggtgccaaaa tcgtccaaag ccaaagagag ccccttgatc atctctgctg
      421 aaaaggagct gttcttgaac agttgaacaa tgttttcata gtcggcacga atctggctca
      481 gttccttgta tttgtattcc ctgagttctt gcaggtgatt gtggttgatg aaatcgatca
      541 cgccatctga agcaatgtac caggttttag gggccttgat gccggccagc tccgggaaat
      601 tcttttgttc ctccttgatg atgttcatcg caatgaacag tcctgcgcct ttcccgccca
      661 ctttgccatc gcttgccggc gtgaaaatga ggcgtttggt caaatcataa aaatcatcaa
      721 tcgtgatgtg tgttttggca atattgataa aatccagacg acgactgaaa aagcgcctga
      781 tgagcgaaac attgatggcg ttcatggtgg aaaaagggag ttcctgctcg ctgagttggg
      841 agttcttgaa tctgacgatc gcgtcaatga tgtcgcccag cgatgaccgg ttgtcgtcca
      901 acaccttgac cagaaaactc agtttgtttt ccttgaccca tttttgcacg agcgaaaaaa
      961 tctgctcgtc tcccaaaatg gattcagcga gagcaaaaat tttttcggcc atgagaggat
     1021 caattttata aatacgtttt ttcaaggggc ggttggtgtc aatttctgca gtgtaaagtg
     1081 tgtcgggaga aacaggcaaa gagccgccaa tttcattaat caggagcctg gcctcctcgc
     1141 ggcccttgga ggccagatga ttcaggaggc gacgagagat gttaaaataa aactgt
//
LOCUS       DATDUN010000036         1028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_209182, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000036 DATDUN010000000
VERSION     DATDUN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209182"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VJC18_00465"
     CDS             complement(<1..233)
                     /locus_tag="VJC18_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLD43878.1"
                     /translation="MNLILHVWRQKNASAKGKLVMYFAHNIGPDMSFLEMLDFVNEGL
                     VEKGEDPIAFDHDCREGICGQCSCVINGKAHGP"
     gene            complement(323..>1028)
                     /locus_tag="VJC18_00470"
     CDS             complement(323..>1028)
                     /locus_tag="VJC18_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HLD43879.1"
                     /translation="MGGLWVDYNLMSNVPGLFVLGEANFSDHGANRLGASALMQGLAD
                     GYFVLPYTIGNYLTGIEQKLPSIDHQDFVDAELLARDQSKKLLSINGTRTVESFHRDL
                     GKIMWEKCGMGRNEKGLKEALVDIPKLKEDFWKNVRVLGTNEELNYSLEKAGRVADYL
                     DFAQLMCLDALTRDESCGGHFREEHQTKEGEALRNDRSFAHVSVWEHVADNKQPVLHK
                     EPLVFEEIKLAERNYK"
BASE COUNT          242 a          275 c          248 g          263 t
ORIGIN      
        1 tccgggccgt gggccttgcc gttgatcacg caagaacatt gtccgcaaat gccctctctg
       61 caatcgtgat caaaggcaat ggggtcctcg cccttttcaa ccaagccttc gttgacaaag
      121 tcaagcattt ccaaaaaaga catgtcggga ccgatgttgt gggcaaagta catcaccagt
      181 ttgcctttgg ccgaagcgtt tttttgtcgc cagacgtgta aaattaaatt catatcaatt
      241 cacgtggatc gtggatcgtg gttcgtggat cgaaaaaaac aatagcattt tacgattcac
      301 gattcacgat gcacgattca cgctatttgt aatttctctc cgccaattta atctcctcaa
      361 ataccagcgg ttctttgtgc aaaacaggtt gcttgttgtc agccacatgt tcccaaacag
      421 acacgtgcgc gaaagagcgg tcattgcgca gggcttcacc ttccttcgtt tggtgttctt
      481 cacggaaatg cccgccgcag gattcgtcgc gagtgagcgc gtcgagacac atgagttgcg
      541 caaaatccag gtaatcggcg acgcgcccgg ccttttcgag tgaataattc agctcctcgt
      601 tcgtgcccag cactcgcacg ttcttccaga agtcttcctt gagtttgggg atgtcgacca
      661 gtgcctcctt tagccccttt tcattccggc ccatgccgca tttttcccac atgattttgc
      721 ccagatcgcg atggaacgac tcaaccgtgc gggtgccgtt aatggacaaa agttttttgg
      781 actgatccct ggccaggagt tcggcgtcca caaaatcctg atgatcgata gaaggtagct
      841 tttgctcaat gcccgtgagg taattgccaa tggtgtaggg caggacaaaa taaccgtccg
      901 caagtccctg catgagggcg ctggcaccca atcggttggc cccgtgatcc gaaaaattgg
      961 cttcacccag aacaaataga ccgggaacat tgctcatgag gttgtagtcc acccagagtc
     1021 cacccatg
//
LOCUS       DATDUN010000037         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_250881, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000037 DATDUN010000000
VERSION     DATDUN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250881"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VJC18_00475"
     CDS             complement(<1..511)
                     /locus_tag="VJC18_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HLD43880.1"
                     /translation="MSREVFVYEAIRTPRGRGKKDGSLCEVKPINLLAGLLRELKERT
                     ALDTTQVDDAVIGCVTPIQDQGMNIGKTALLVADWSESVPGFQLNRFCSSGLAAINLA
                     VAKIRSGWEDLVVAGGVESMSRVPMGSDGGAWANDPEFSIKTGFVPQGVCADLIATME
                     GFSREACDHF"
     gene            764..1372
                     /locus_tag="VJC18_00480"
     CDS             764..1372
                     /locus_tag="VJC18_00480"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HLD43881.1"
                     /translation="MPHALPQLPYSKDALAPFFSAETLEYHYGKHHATYVTNLNNLIA
                     STEWADKPLEDFITNPSALPTDKKAALYNNAAQVWNHTFYWNCLAPKAGGPATGQIAD
                     LINSTWGSFSAFKDAFTKSAIGTFGSGWAWLVKNKDDKLEIVSTSNAQNPLTDGKTPL
                     LTCDVWEHAYYIDYRNARAKYIEGFWNLVNWDFVNANLYSAT"
     gene            complement(1519..2304)
                     /locus_tag="VJC18_00485"
     CDS             complement(1519..2304)
                     /locus_tag="VJC18_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43882.1"
                     /translation="MTQALVLVCACLLVTWDVGAAVSKRPEKASQNGISQPKTKAEAV
                     IRSEAGLNIQEWGIAIDAIFDPRLDDLIPGYHVVNLVLTNRRGEPIELNTKQDKWYIV
                     DSLGKKHTAYNHVKQFKDALWPKLPQKMRDMLEYPHIVNAGKSTNIDVFLPKSVDLFH
                     FREVIWKSAHFNKEFNILTNYEKELSIDQKKEFDLPKKITAEEELLMEENRGQLNPEA
                     ASQVSTPPNTPENSQPQALELNEQGTSNTSTPQASVEGFIRVK"
BASE COUNT          564 a          645 c          563 g          594 t
ORIGIN      
        1 caaaatgatc gcaagcttcc cggctgaacc cttccatcgt tgcgatgagg tcagcgcaga
       61 ccccctgcgg aacaaaccct gttttgatac tgaactcggg atcattggcc cacgcccctc
      121 catccgagcc cataggcaca cgagacatcg actcgacgcc ccccgcaacc accaggtctt
      181 cccaacccga acggatcttt gccaccgcga gattgatggc ggcaaggccc gatgaacaaa
      241 accggttgag ttggaatccc ggcaccgact cgctccagtc ggccacaagc agtgcggtct
      301 tgccgatatt catcccctga tcctgaatgg gagtcacaca gccgatcacc gcgtcatcca
      361 cctgcgtagt atccagtgct gttcgctcct tcaattcgcg cagaagccct gccagcagat
      421 tgattggttt gacctcgcac agagagccgt ccttcttgcc cctccctcgg ggtgtcctga
      481 ttgcttcgta aacaaagacc tctcggctca taagaacctc gtgggttgat tgttgatgat
      541 ctgctcgggg aagcctaccg agaagccaca ggggtgtcaa acagcaaaga cagaagcaaa
      601 gacagaaggg gtgtgatatc gacttgactt cggttatggc tcctgtcatt cgtgcaattg
      661 ttccgccgtt gtttcttgat tgcttcattg cttgattgct tgattgcttg attgcttcat
      721 tgcttgattg cctcattgat tcattttccc caaggagccc cttatgcccc acgcgctacc
      781 ccaacttccc tattccaaag acgccttggc cccgtttttt tcggctgaaa ccttggaata
      841 ccactatggc aagcaccatg ccacttacgt gaccaatctg aacaatctga ttgcaagcac
      901 agaatgggca gacaaacccc tcgaagattt catcaccaat ccctctgcct tgcccaccga
      961 taaaaaagca gccctctaca acaacgcggc acaggtttgg aaccacacgt tttactggaa
     1021 ttgtctggcc cccaaagcgg gcggccctgc cacaggacaa atcgcagacc tcatcaacag
     1081 cacttggggc tcgttttctg cgttcaagga cgccttcacc aaatcggcca tcggcacctt
     1141 tggatcaggc tgggcctggc tggtgaaaaa caaagacgac aagttggaaa ttgtctcgac
     1201 gtccaacgca caaaatccct taacagacgg caagacacca ctgctgacct gcgacgtgtg
     1261 ggaacacgcg tactacatag attaccgcaa cgcccgagcc aaatacattg aggggttttg
     1321 gaatctggtc aattgggatt ttgtgaacgc taatctatac tcagcaacat aagttcgaga
     1381 cgcttttgag gcaaggagtg aaatgaggag ccagcggagc gtacacgcaa gtacgtgagc
     1441 aggcaccgca atgaacgaca cagcatcaaa agatgtatcg gacttatgtc gctgagtata
     1501 caataagggg tgacaatatt attttacccg gataaaccct tcaaccgagg cctgcggagt
     1561 gctggtgtta gaggttcctt gttcgttaag ctccaatgcc tgaggctggc tgttttcggg
     1621 ggtattgggt ggggtggaca cctgtgaggc ggcttctgga tttaattgcc cgcgattctc
     1681 ttccatgagc aattcttctt cggcagtgat ttttttgggc aaatcaaatt cctttttctg
     1741 atctatcgac aattcctttt cgtaattcgt caggatgttg aattctttat tgaagtgtgc
     1801 cgatttccag atcacttccc tgaagtgaaa cagatcaacc gactttggca aaaacacatc
     1861 aatgttggtg gacttgcccg cgttgacgat atgcgggtat tccagcatgt cccgcatttt
     1921 ttggggaagc ttgggccaca aagcatcttt gaattgttta acatgattgt aggccgtgtg
     1981 tttttttccc agactatcga cgatgtacca cttatcctgt ttcgtattca gctcgatcgg
     2041 ctcaccccgt ctgttggtga gcaccagatt gacaacatgg taccccggaa tcaggtcgtc
     2101 caaacgagga tcaaaaatgg cgtcgatggc aatgccccat tcctgaatgt tgagcccggc
     2161 ttcactgcga atcacagcct ctgcttttgt tttgggttgc gaaatgccat tctggctggc
     2221 tttttcggga cgcttgctca ccgccgcgcc gacgtcccac gtaaccagca gacacgcaca
     2281 aaccaacacc agagcttgag tcatatttct catatgaagt attgatccag aatttttcgc
     2341 gtcagggtat tgatgggcaa agcctt
//
LOCUS       DATDUN010000038         1967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_250948, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000038 DATDUN010000000
VERSION     DATDUN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250948"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1417
                     /locus_tag="VJC18_00490"
     CDS             407..1417
                     /locus_tag="VJC18_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HLD43883.1"
                     /translation="MLAIQTDATGKSMTVNISKPHIGPDEVLIKTIGCGLCGTDLLKI
                     NLGLLRQPTILGHEVVGRVAEKGAKVFDYQLGDLIVVAHHVPCGTCHYCLHGNHSMCA
                     TFKKTNLDPGGFCEFIRLSKQHLDHTTFKITGDFAWQEALFTEPLACCERSFQKLTIL
                     PGDVLVIVGLGSIGIMMTKLVKRLGGKVIGIDTDTHRCSQALSYGADKTIESTDKLHT
                     LINEPTQSRGADGIIFTAGPAGLLNASLLWLRDGGFVNLFSHLSGEVGPIDTSRLYHH
                     EIQIITSYSATPESLKRSFDILRSEHLNLKQMFETYRPDQFEQAVKDMNERKVYKALI
                     EF"
     gene            complement(1606..>1967)
                     /gene="carB"
                     /locus_tag="VJC18_00495"
     CDS             complement(1606..>1967)
                     /gene="carB"
                     /locus_tag="VJC18_00495"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HLD43884.1"
                     /translation="DKRAVASLAARLVDHGFTLVATGGTAIFLKRHGLDVDIVKKHYE
                     GEPNILQALLNKEIAMVINTHEGKLSAVDSYEMRRTALVTNTLYFTTIAAAEAAVMGF
                     VALQKGPMQVKPLQEYY"
BASE COUNT          494 a          539 c          454 g          480 t
ORIGIN      
        1 aggatgggat ttataggcta aaaggttcag gcacataaag aagcatctcc tctactcagc
       61 gacggcaggt atcgcaataa aacactactc agcgacggca ggtatcgcaa taaaacaatt
      121 gcttcaatag taatcggtca ctcattggag cgttttgatt ttttgggtcc tgctccgggt
      181 cgctgttttt tgacgctctt cgcaagcggg gcttgctccg caggccgcat caaaataata
      241 gctccccgta tcaccccaaa aatcaatacg ctccgttcac tgacgcctta ccttcaataa
      301 tcaattgcca atttgagtag ttgataattg caataattga agcaattgat tctttcattg
      361 tcctttgact ttgacctttt agcttcgtgc taatatatat ccccccatgc ttgccataca
      421 aaccgatgcc accggcaaat ccatgacagt caacatttcc aaaccacata ttggtcccga
      481 tgaggttctc atcaaaacca ttggttgcgg cttgtgcggc acagatctgt taaaaatcaa
      541 cctgggattg ctgcgccagc ccaccatttt gggccatgag gttgtcggtc gcgttgcaga
      601 aaagggagcc aaggtgtttg attaccagct cggggatctc atcgttgtgg cccaccatgt
      661 tccctgtggc acatgtcatt actgcctcca cggcaaccac tccatgtgcg ccaccttcaa
      721 aaaaaccaat ctcgatccgg gtggcttttg cgaattcatc cgcctttcca aacaacacct
      781 cgaccacacc accttcaaga tcacgggtga ctttgcgtgg caggaggcct tgttcacgga
      841 accactggcc tgttgcgaac ggagctttca aaaactcacc attctcccgg gtgatgtgct
      901 tgtgatcgtg gggttgggct ctatcggcat catgatgacc aagctcgtca aacgactggg
      961 tggcaaagtc attgggatcg ataccgacac acacaggtgc tctcaagccc tctcctatgg
     1021 tgccgacaaa acaattgaga gcactgataa actgcacacc ctcatcaacg agccgactca
     1081 gagcagaggg gccgacggca tcatcttcac cgcgggacca gcggggcttt tgaacgcatc
     1141 cctcttgtgg ctcagggatg gcggctttgt aaatctcttc agtcacctct cgggagaggt
     1201 aggccccatc gatacatccc gcctgtatca ccacgaaatc cagatcatca ccagttattc
     1261 tgccaccccc gaatccctga aaaggtcttt tgacatcctc agaagtgagc atttgaattt
     1321 aaaacaaatg tttgaaacgt accggccgga tcaattcgaa caggcggtca aggacatgaa
     1381 tgaacgaaag gtgtacaagg cgttgattga gttttgaaac ggtgttcatg agaacatttc
     1441 gtacgggtga ctggcgaagc tattattccc aatcgatccc acagcagtgg gacgagcgaa
     1501 cttgtgagcg aggagattgg gaaaatagca agccaaggca ggacccgtag aaatttcctc
     1561 atgaacactg tttcttgcag aaagaaccca tgaccctgct gtgtctcaat aatactcctg
     1621 caacggcttg acctgcatcg ggcctttctg cagggccaca aagcccatca ccgcggcctc
     1681 cgcggccgcg atcgtcgtaa agtacagggt gttggtcacc aatgctgtcc ggcgcatttc
     1741 ataggagtcg accgccgaga gtttcccttc atgcgtgttg atcaccatgg cgatttcctt
     1801 gttcagcagg gcctgcagaa tattgggctc cccttcataa tgttttttaa caatgtctac
     1861 atccaatcca tgtcgcttca aaaaaatcgc cgtcccccct gtcgccacaa gcgtgaatcc
     1921 atgatcgacc agcctggccg ccaaggaagc cactgcgcgc ttatcac
//
LOCUS       DATDUN010000039         1148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_167974, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000039 DATDUN010000000
VERSION     DATDUN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167974"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VJC18_00500"
     CDS             complement(<1..358)
                     /locus_tag="VJC18_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLD43885.1"
                     /translation="MLHWVKKHQIIIGDFALICMTVIWGSTFFVVKDQLRFIDPVTMV
                     FYRFAAASLILGLYLFFRRIPLFHNWRRGLIVGFWLWVIYVAQNIGMLYTTASNSGFI
                     TALNIVFVPILSLMMFR"
     gene            562..>1148
                     /locus_tag="VJC18_00505"
     CDS             562..>1148
                     /locus_tag="VJC18_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLD43886.1"
                     /translation="MTCSVVASAQTTLRTPNDIVRAITASNYDLKAMSENTRAARATL
                     NALKGSFYTPQISSTASYQDSQSQPLSPFEPASYQTLFWQTYLQSRTPYGVSVGTGHS
                     FSRYHFGDASPPNPLLPYPITYYQPETYVQISLDLTQDFLGYLSSKQIEMAQLDADAA
                     KLQEALVRHKFTALALDLYYQLPVLKSVRGYATAM"
BASE COUNT          316 a          343 c          249 g          240 t
ORIGIN      
        1 gcctgaacat catcagtgac aaaatgggga cgaaaacgat gttgagcgcc gtgatgaaac
       61 ctgaattcga ggccgtggtg tagagcatgc caatgttctg cgccacataa atcacccaca
      121 accagaaccc cacaatgagc ccacggcgcc agttatgaaa cagggggatc cggcgaaaaa
      181 acaaatacag gcccagaatc agggacgcgg ccgcaaagcg ataaaagacc atcgtcaccg
      241 gatcgatgaa tcgcagttgg tccttgacca cgaaaaaggt cgaaccccaa atcacagtca
      301 tgcaaatcaa cgcgaaatca ccgatgatga tctgatgttt tttcacccag tgcaacatgg
      361 ggcctcatag ggaaagccca ccgaaaaagc aatgcctgtc tgcccaaatg gttttcaaaa
      421 ttcacttgtc ccttttcaaa caaatgtaat aaagttgatc acgctcgcaa ccggcaacac
      481 ttcatcaagc gaactgttgc aataactggt cctgcaaaaa tgaggaattt cgtcccaagc
      541 atattatccc tcgctgtctt gttgacctgt tctgtcgtcg cttctgcgca aacgaccctg
      601 cgcaccccga acgacattgt gcgtgccatc accgccagca attacgacct caaggcgatg
      661 tccgaaaaca cccgcgccgc acgggcgact ctcaatgccc tgaaaggcag tttttacaca
      721 ccacaaattt cgtcgacagc gtcctatcaa gacagtcaga gccaaccgtt gagccccttc
      781 gaacccgcca gttatcaaac gctgttttgg caaacctatt tacaatcacg cactccttac
      841 ggcgtgtccg taggcacggg acacagtttt tcgcgctacc attttggtga cgcctcgcca
      901 ccaaacccgc tcctgcccta ccccatcacc tattatcaac ccgagactta cgtacaaatt
      961 tcgctggacc tcacgcagga ttttttgggc tacctttcct ccaagcagat cgagatggcc
     1021 caactggatg ctgacgcagc caagcttcag gaggcactgg tcaggcataa attcacagcc
     1081 ttggcgctgg atctgtacta ccaactcccg gtcctcaaaa gcgttcgcgg ttacgcgaca
     1141 gccatgat
//
LOCUS       DATDUN010000040         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_43370, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000040 DATDUN010000000
VERSION     DATDUN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43370"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VJC18_00510"
     CDS             <1..284
                     /locus_tag="VJC18_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HLD43887.1"
                     /translation="LFKRMARNMQTELVAEIYRGAGELLQVKHPLLWLKIHGYKKLLR
                     QAGKEVVLQSRLSVATQQKLEEPLIDSPTYVKHCNEWVDKAVKRSASIK"
     gene            complement(332..607)
                     /locus_tag="VJC18_00515"
     CDS             complement(332..607)
                     /locus_tag="VJC18_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43888.1"
                     /translation="MPNTCQIGALYFDRETASICANNLERGSVVKINDCSKLSIESTD
                     VYCVGDNPTYVVVQKDNFWTSFKKFAHGFSANFGAAGSDPYCATPGA"
     gene            complement(1070..1681)
                     /locus_tag="VJC18_00520"
     CDS             complement(1070..1681)
                     /locus_tag="VJC18_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HLD43889.1"
                     /translation="MHSLINWLLTTIGALGYPGIVFLMALESSVFPVPSELVMPPAGY
                     LVSQNQMNLFLVILCGTVGSLIGAYANYFASRYVGRPFILKYGKYVLISEAKLIRAET
                     FFRKHGAISTFVGRLFPVVRHLISIPAGLAGMNHVTFSLYTLLGSFIWVSILTVLGYY
                     IGTNQALINEYLTPILIIVGILCALIVAGYVWLHRRRTKSIEA"
     gene            complement(1802..>2599)
                     /locus_tag="VJC18_00525"
     CDS             complement(1802..>2599)
                     /locus_tag="VJC18_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43890.1"
                     /translation="LRDREIKAYFSALAQDVETGTRIADSCLKEIQMQHVRTQVTQIQ
                     HVADDFKIISFDLNLNSLAGQFVFVWLPGVAEKPFSVLDDNPLTIAVQERGKCTRAIN
                     ELKAGDPVYIRGPHGTGFTKLSSHHLVLVGGGSGIAGLYLFAKHFASTHKITTLIGTK
                     DDNHIFYKKEFEQCGDVLIATEDGSLGKKGFVTELFSDVCFDRETQFINCGPLPMIEA
                     VTKIESQITTGEHILSSIDYLTMCGVGICGTCADSHGTRSCVEGPFY"
BASE COUNT          719 a          636 c          628 g          616 t
ORIGIN      
        1 tattgttcaa acgcatggcc cgtaacatgc aaacggaact ggtggccgaa atttaccgcg
       61 gggcgggtga actcttgcag gtcaagcatc ccttgctctg gctcaaaatt cacggctaca
      121 aaaaacttct ccgccaagca ggcaaagagg ttgttttgca atcacgtcta tctgtcgcaa
      181 cacagcaaaa actcgaagaa cccctcatcg attccccaac ctatgtcaaa cattgcaacg
      241 aatgggtgga taaggcagtc aagcgatcag cgtcaatcaa atgattaatg gttatcaaca
      301 caacactata ttcgattgac aaaagttgtt ctcaagcccc cggggttgca caataaggat
      361 cactgccggc cgcgccaaaa ttcgcggaaa atccatgagc aaatttcttg aaagatgtcc
      421 aaaagttatc tttctgcacc acaacgtagg tgggattatc ccccacacaa tagacatccg
      481 tagattcgat gctcaatttc gaacagtcgt ttattttcac aacggaacct ctttcgagat
      541 tgttcgcgca gatgctggct gtttctctat caaaatacag cgcaccaatt tgacaggtat
      601 ttggcataaa ggcctccttg aagggattga atgctagtgt tgagcagtgt cactatactc
      661 agcgacataa gtccgataca tcttttgatg ctgtgtcgtt cattgcggtg cctgctcacg
      721 tacttgcgtg agcgctccgc tggctcctca tttcactcct tgcctcaaaa gcgtctcgaa
      781 cttatgtcgc tgagtataga taaaatttaa tctgtcgaag aactagtcca agtcaaaata
      841 gagttcaagt ttttttcaat ccagaagacc gccactgcaa caccccgtcc acaattgtgt
      901 tctctttacc cccgatgcgt agggcgatat gggtctttgc cggtgtgcgt gttgatctta
      961 tgaagaacgc aggcacgtgc acatcatagg agcacaccat gattaccgca agacccctca
     1021 gcccttattt gttgggtgag ttgaatcata aaatataatc ccattctact cacgcctcga
     1081 tgcttttggt cctgcgccgg tgtaaccaga cataccccgc cacgatcagg gcgcataaaa
     1141 tgcccacgat gatgaggatg ggggtgaggt actcgttgat gagggcctga ttggttccaa
     1201 tgtaataacc caacacggtc aggatgctga cccaaatgaa cgagcccaag agggtgtaga
     1261 ggctgaaggt cacatggttc atcccggcca gtcccgccgg gatcgaaatg aggtggcgca
     1321 caaccggaaa aagcctgccc acaaaggtgg agatggcgcc gtgctttcta aaaaaagttt
     1381 ccgcccggat cagtttggcc tcagaaatca aaacgtattt gccgtacttg aggataaaag
     1441 gcctgcccac ataacgagag gcaaagtaat tggcataagc gccaatgagg ctccccactg
     1501 ttccacagag gatcaccaaa aacaggttca tttgattttg agaaaccaga taacccgcgg
     1561 ggggcatgac caactcactg ggcacgggaa agacagagct ttccaacgcc atgagaaaca
     1621 caatgcccgg gtaacccaag gccccaatgg tggtgaggag ccaattgatg agtgagtgca
     1681 tgggagggca ataataggtc aatggtcaaa aggtcaatgg tcaaagggta aaacgtgttc
     1741 acggaaaatt tctcatcgta agcacgtgaa tacgtgaaca cctgaatacg agcgaagcga
     1801 ttcaataaaa tggcccctcc acacaggaac gtgtgccgtg agaatcggcg caggtgccgc
     1861 aaatgcccac cccacacatg gtgaggtaat cgatggatga gagaatgtgt tcacccgtag
     1921 tgatttgcga ttcaattttg gtgacagctt caatcatggg caggggaccg caattgatga
     1981 actgtgtctc gcggtcaaaa cacacatcac taaacaactc tgtcacaaaa cccttcttgc
     2041 ccagagaacc atcctctgtg gcgatgagca catcaccgca ttgctcaaat tcttttttat
     2101 aaaaaatgtg gttgtcgtct ttggtcccga ttagcgtggt gatcttgtgt gtggaggcaa
     2161 agtgcttggc aaaaagatat agtcccgcaa tgccagaccc gccgccaacc agaacaagat
     2221 gatgactgct caattttgtg aagcctgtac cgtgtggacc gcgaatgtaa acgggatcgc
     2281 ccgctttcaa ttcattgatg gcccgggtgc atttgcctct ttcctgcacg gcgatcgtga
     2341 gagggttgtc atccaaaacc gaaaatggtt tttcggccac gccgggtaac cagacaaaca
     2401 caaattgtcc agccagagag tttaaattga gatcaaaaga gatgatttta aaatcatcag
     2461 ccacgtgctg gatttgggtc acctgggtgc gcacgtgttg catctggatt tctttgagac
     2521 aggaatcagc gattcgggtt cctgtttcca cgtcttgggc gagggcagaa aaatacgcct
     2581 tgatctcacg atctcgcag
//
LOCUS       DATDUN010000041         4315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_168035, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000041 DATDUN010000000
VERSION     DATDUN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168035"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..839)
                     /gene="dcd"
                     /locus_tag="VJC18_00530"
     CDS             complement(330..839)
                     /gene="dcd"
                     /locus_tag="VJC18_00530"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HLD43891.1"
                     /translation="MSVIAQSELRKLLKAGVIKVEPCDDSQIGPASIDLHVGNTFLVY
                     KKARGICHVTDDANFEDITEEFTVNDYFVLMPGESVLAYTKEKVTLPDNICGRLEGRS
                     RFARLGLLVHITASFMQPGISNRQILEMYNASQFPLAIHTGTRICQFIFEFTEGTGHY
                     TGRFVNQGE"
     gene            1245..1592
                     /locus_tag="VJC18_00535"
     CDS             1245..1592
                     /locus_tag="VJC18_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HLD43892.1"
                     /translation="MPLLKLSTNANIKDEQKSELLKSLSSFLAAATGKPEQYVMVVIE
                     RADILMSGGGGDSAFVEVKSLGALEPFTVKKLSKKICDLLSASLDISPVRVYINFQDV
                     PASQWAWNGKPFG"
     gene            1619..1849
                     /locus_tag="VJC18_00540"
     CDS             1619..1849
                     /locus_tag="VJC18_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43893.1"
                     /translation="MEIKNLCASPTLILISETERHLALLEDVAIDSKVDGNLMFDAHI
                     ATLLVEHGVREIATSDNDFKRFKELTVVNPVE"
     gene            2011..>4315
                     /locus_tag="VJC18_00545"
     CDS             2011..>4315
                     /locus_tag="VJC18_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43894.1"
                     /translation="MTPISAINSLPAEISPQEETVLNKVLGDAVKDGIITRADIEQYY
                     RQIDQQNYNFAEFVTVENILRKHGYTPPADEEIVVTFDEIRQSRDEVRQTLRENGYQI
                     HRTTKDGNSVYLPPFGQRHKPKVIITESGVCVLKRQGIRFKKPENPLDILKIFVNPTA
                     FIQGSGLLVRKSIIDQQRALLLISLEPYLNHYHNTIARDSLQKNLFTLQAELETFWFG
                     PAVTQFTVLDVSLLDQALSPEEKRRLILEQWQQVPERDQIRDGQIYSEDKSDELIRTV
                     FSLFIENIIMVSSWPYTEPEIAQANEKRVCGEPLRVDFSSEPKSVSTLSDAGLYQQTS
                     AQDIWDQLSRFEKAQALEDKGKYEEALKYYLEVLKTEDLALVFVEDNGEPVAKKEVRF
                     ILQERIGRCRLADKITKELMMARTQYQMALNYFEQGDLGEAKQQLKKAFGSNPFITLD
                     ERPVVSVCLAPDKQIRLSESSASVKAIKNRYEDMHFYPHVYEDVVLRSFDARLFSDGL
                     ILMGKILDRSDRHEFAALYFDAATTFLLPYHMTHPEDKEMGEYFAGIPGADQQMVQRL
                     AWQAESERYLRETRQAQVESKIPSAPVEKTSAEYSWKEVHWAVVGILNEAQQKTPVAG
                     DESAITAELEKGFSLLLDFLIRKSGQDPKREFSLPELFGALAQQSSQSELTDAVKLLY
                     RFAQLLGEDDVVISACSYLATFREDPDDRGYYSLRLAELARERGDETLVAKALAGVAK
                     NTDDADTLSAARDMASHQETPFGRIIYE"
BASE COUNT         1185 a          972 c         1040 g         1118 t
ORIGIN      
        1 ctgtccattg tgcactaaca cggctccaat agggatgcca ccttcggcta aacttttctt
       61 ggcttcttcg atggcggatg tgagaaaatc aatcataacg tgttcatgtg ttcacgaaaa
      121 acagggctta aggcgtaagg cttgaggctt aaggctgaaa caaggaacaa gaaatatcga
      181 aaggagtgtc attcctgcgc atgcaggaat ccatgtttgc aataattctg gatccctgcg
      241 ttcgcaggaa tgacaaattt caacattcga cattccttgt tcgatattca atattcaaag
      301 tcctttgagc tgtcttcgtc cctcgtccct cactccccct gattcacaaa acgtcctgtg
      361 taatgccctg tgccttcggt gaactcaaaa ataaactggc agatgcgcgt gcccgtgtgg
      421 atggccaaag ggaactggct cgcgttgtac atttctaaaa tctgtcggtt ggaaatgccc
      481 ggttgcatga aactggcggt gatgtggacc aacaaaccca agcgggcgaa gcgactgcga
      541 ccttcgaggc gaccacaaat gttgtcgggc agagtcactt tttccttggt gtaggccagt
      601 accgattcgc cgggcatcaa aacaaaataa tcattgaccg taaactcttc ggtgatgtcc
      661 tcaaagttgg catcgtcggt cacatggcaa atgccgcggg cctttttgta gaccagaaag
      721 gtgttgccaa cgtgcaggtc gatggaggca gggccgattt gggaatcgtc acagggctcc
      781 accttgatga cgccagcttt caatagtttt ctcagttcac tttgggcaat gacactcata
      841 tttgaatagc gaataacgaa caaggaacaa ggaatatcga aggaatgaat caatcaagaa
      901 atcaagaaat gaagtaatga aataataaaa ttaaccagcc tttgtatcgt tgtttatctc
      961 ttgattactt cattacttca ttgattcatt gattcattga ttcatccccc ccttcgatat
     1021 tccttgatcg atattcgata ttcgtctgta attatttgtt gtccgttctt tttatccata
     1081 ctatgttatt tttgtaaaga ctttagtcca atgtcttacg tgtttacgga aaggcgtaag
     1141 gctacgggct cccgctaaca gcctactgct aacggccaac ggcaatttat tggctgtgag
     1201 ctgttggccg taaactgtta gcccgcattt gagcttcgta ttctatgccc ctattaaaac
     1261 tcagcaccaa cgccaacatc aaggacgaac aaaaaagcga gctcctcaaa tccctctcga
     1321 gttttttggc cgccgccact ggcaaaccgg aacagtatgt gatggtggtc attgaacgcg
     1381 ccgatatcct catgtcgggt gggggaggcg attctgcctt tgtggaggtc aagagcctgg
     1441 gtgcccttga acctttcaca gtcaaaaagc tatccaaaaa aatctgtgat ctgttgtctg
     1501 cctctttgga tatcagtccc gtcagggttt atatcaattt tcaggacgtt cctgccagtc
     1561 agtgggcctg gaatggcaaa ccgtttgggt aaagggcaca ccactccaga ctgcggtttt
     1621 ggaaatcaag aatctttgtg cttctccgac gctcatcctc attagcgaga cagaaaggca
     1681 tttggcatta ttggaagatg tggcgataga ttccaaggtg gatggcaacc tcatgttcga
     1741 cgcacacatt gccacactac ttgtggaaca cggtgtgcgc gaaattgcca cctcagataa
     1801 cgattttaaa cggtttaaag aattgaccgt cgtgaatcca gttgagtgat gagagtaatt
     1861 gcctgtttca tccaccaaaa ccatgcttac cccatcaaaa aaacgtccac aactgtggac
     1921 atgttatttt cgaattcaag caactatttt ttattttaac cgataatctg cctgaagggt
     1981 cagacaacgt gatcgagttt atcaacgcat atgacaccaa tcagtgctat aaactctctc
     2041 cctgccgaga tatcaccgca ggaagaaact gtgctcaata aggtattggg agatgccgtc
     2101 aaagacggga ttatcaccag agcagatatt gagcagtatt atcgccagat tgatcaacaa
     2161 aattataatt tcgccgagtt tgtcaccgta gaaaacattc tcagaaaaca cggatacact
     2221 cccccagccg atgaagagat cgtggtgacc tttgatgaaa ttcgccagtc acgtgacgag
     2281 gtgcgtcaaa ccctgagaga gaatgggtat caaatacacc gcaccacgaa agatgggaat
     2341 tctgtgtatt tgccgccgtt tggacagaga cacaaaccca aggtcatcat cacagaatcg
     2401 ggtgtctgtg tgctcaaaag acaggggatt cgttttaaga aaccggaaaa cccgctcgat
     2461 attctaaaaa tctttgtcaa cccgacagcc tttattcaag gatcgggcct cctggtcaga
     2521 aaatccatta ttgaccagca gagagcctta ttactcatca gccttgagcc gtatcttaat
     2581 cattatcaca acacgatagc ccgcgacagt ctgcaaaaga atctcttcac actgcaagcc
     2641 gaactggaga ccttctggtt tgggccagca gtcacacaat tcactgtgct ggatgtgtct
     2701 ttgttggacc aggcgttgag tccggaagaa aagcgcaggc tcattctgga acagtggcaa
     2761 caggtccccg agcgagatca aatcagagac ggtcaaattt attccgaaga caaatcagat
     2821 gaacttatcc gaacagtatt ttcccttttt atagaaaaca tcatcatggt atcttcctgg
     2881 ccatatacag aaccagagat cgctcaggcc aatgagaaac gcgtttgtgg tgaaccgctg
     2941 agagttgatt tttcttcgga gcccaagtct gtttccactt tgtctgacgc aggattgtac
     3001 cagcagacaa gcgctcaaga tatttgggat cagttaagcc gttttgaaaa agcgcaggcg
     3061 cttgaagata agggaaaata tgaggaggcc ctcaaatatt atctggaggt tctgaaaact
     3121 gaggatctgg ctttggtgtt tgtggaggat aacggggagc cggtggcaaa aaaagaggtc
     3181 aggtttattt tgcaagaacg catcgggcgt tgccgtcttg cagacaaaat cacgaaagag
     3241 ctgatgatgg cacggactca gtatcaaatg gccctcaatt attttgaaca gggggatttg
     3301 ggtgaagcga agcaacagct taaaaaggcc tttggatcca atcccttcat taccttagat
     3361 gaaagacctg ttgtatcggt ttgtctggcc cccgacaaac aaataaggct cagtgaatcg
     3421 tcggcatcgg tcaaggcgat aaaaaataga tatgaggata tgcattttta cccgcatgtc
     3481 tacgaagatg tggtgttgcg ttcgtttgat gccaggttgt tttcggatgg tcttatcctc
     3541 atgggcaaaa ttctggatcg ttcagatcgg catgagttcg cggctttgta ttttgatgcg
     3601 gcgacaactt tcttgttgcc ctatcatatg acacatccag aggataaaga gatgggcgaa
     3661 tattttgcgg gtatccctgg cgcagatcag caaatggtcc aaagattggc ctggcaggcc
     3721 gagagcgaac gttatttgcg cgaaacccgc caagcccaag tggaatccaa gattccctct
     3781 gcccccgttg agaagacgtc agcagaatat agctggaaag aagtccactg ggcggtggtg
     3841 ggaatattaa atgaggcaca acagaaaaca ccggtagcgg gtgatgaaag tgccattacc
     3901 gcagaactgg aaaaagggtt ttccttgttg ctggactttt tgattagaaa gagtggtcag
     3961 gatcccaaaa gggaattttc cctgccagaa ttgtttggtg ctttggccca acagtcctca
     4021 cagtccgaac tcacggacgc agtcaagttg ctctatcgtt ttgcacaact gctgggagag
     4081 gacgatgtgg tgatttcggc ctgttcctat ctggccactt ttagggaaga tcccgatgac
     4141 aggggatatt attctctcag gctggcggag ttggcaagag aaaggggtga tgagacactc
     4201 gtcgccaaag cactggcagg cgttgccaaa aatactgacg atgccgacac gctttctgcc
     4261 gcacgggata tggcaagtca tcaagaaact ccatttgggc gcatcattta tgaac
//
LOCUS       DATDUN010000042         1820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_126251, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000042 DATDUN010000000
VERSION     DATDUN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126251"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VJC18_00550"
     CDS             <1..1265
                     /locus_tag="VJC18_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLD43895.1"
                     /translation="SRLADDAVYRLYKLSLSRKDTAQSDKYYQILKHLKTGDMKSELA
                     FELAYEQFKKKNFDKSASVLSEALDTIPTADESYTKTLYWYARSLEKTTGRKNHDKAQ
                     STYHRLITEFPYSFYAVLASHRVNSPITLPQRPIIIGEAPTENTTTFELIDAFNKKGF
                     HQAARIVLDFALHEHPQWLLSHKEYLTLKLIESQNYRKALALASDHFKSGPYGPLTIT
                     DDPLFTAFYPLPFKSKTKIAYAKTNLPKGAIEGIMREESLFQRTVSSHAGAIGLMQLM
                     PQTAAQVQKGLIEKGAPTTDFSSDLTDTKTNILLGATYLDQMITKFKNHLPLAIMAYN
                     AGPGNVRKWLQRLGPLELDEFIENIPYTETQGYVKRVLRSMHVYGDLYNNTYFTKGED
                     INFTLPVIKPSPKKHKHHKVNKKKGKKQ"
     regulatory      1298..1419
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1569..>1820
                     /locus_tag="VJC18_00555"
     CDS             1569..>1820
                     /locus_tag="VJC18_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HLD43896.1"
                     /translation="MDHLIMGRSLGLTLFVGTLVSTWYGGIFGVTEIAFNQGIYNFVT
                     QGFFWYLTYLIFAFCLVDKIRQYEAITLPELIKKMFGPRA"
BASE COUNT          516 a          479 c          387 g          438 t
ORIGIN      
        1 acagccggtt ggccgatgac gctgtttacc gattatacaa actgtctttg tcccgtaaag
       61 acacagcgca gtccgacaag tattatcaaa tcctcaagca ccttaaaaca ggcgacatga
      121 aaagcgagtt ggcctttgaa ctggcctatg agcaatttaa aaaaaagaat ttcgacaaat
      181 ccgccagcgt tctgtctgaa gcgctcgata cgatcccgac agcagacgaa tcttatacca
      241 aaacattgta ctggtacgcc cgttcgctcg aaaaaactac tggccgcaaa aatcacgaca
      301 aggctcagag cacttaccat cgactgatca cggagtttcc ctattctttt tatgccgtcc
      361 tggccagcca tcgcgtgaac tcacccatca cgcttcctca acgacccatc atcatcggag
      421 aagcccccac cgagaacacc acgacatttg aattgatcga tgcctttaat aaaaagggct
      481 ttcaccaggc cgcccgcatc gttcttgatt ttgctttgca tgaacacccg cagtggctac
      541 tgagtcataa ggaatatttg accctcaagc tcatcgaatc ccaaaactac cgcaaggcgc
      601 tggccctggc ctccgatcac ttcaaaagtg gtccctatgg ccccttgacc attaccgacg
      661 accctttatt tacggccttt tatcctttgc cgttcaagag caaaacaaaa attgcctatg
      721 caaaaaccaa tctgcccaag ggggccatcg agggcatcat gcgagaagaa tccttgtttc
      781 agcgcacggt atccagccac gctggcgcca ttgggttgat gcaactcatg cctcaaacag
      841 cggcccaggt acaaaaaggc ttgattgaaa agggggcccc gaccacagac ttttcgtcag
      901 atctgactga tacaaaaacc aatattctgt taggggcaac gtacttggac cagatgatca
      961 ccaagtttaa aaatcatctc cctttggcca tcatggccta caacgcaggg ccgggaaatg
     1021 tgcggaaatg gctacaacgc ctggggcctt tggagcttga tgagttcatc gaaaatattc
     1081 catacactga aacacaaggt tatgtgaaaa gggttctgcg ctccatgcat gtgtatggcg
     1141 atttgtacaa caacacttat tttacaaaag gcgaggacat caacttcaca cttcctgtca
     1201 tcaagccaag tcccaaaaaa cacaaacacc acaaggtcaa caaaaagaag gggaagaagc
     1261 aataagaaaa gtaggggtcg gtcgcgaccg acccctacta gtcaatcagg ggagccaatc
     1321 agatcggcca tcattggcca tcaggctgag aggtccatac aggacgaccc taaacttgat
     1381 ccggataata ccggcgtaag gaaacaacat gaattcctat cttcaaccgc aaatcgattt
     1441 tttgaatgtc ttcaccctgc ccgatctcct tgtttttttt ttgcatcgct gtcctgaccg
     1501 tcttggcggt cgtctggggc aatagacaaa aggaaaaaac aaaaaacact tcctctcccc
     1561 actcaatttt ggatcatctg atcatgggca ggagcctggg actcacgctt tttgtgggta
     1621 cgctggtttc gacctggtat ggtggcattt ttggcgtgac agagattgcc tttaatcagg
     1681 ggatctacaa ttttgtcact cagggttttt tttggtacct cacctacctc atttttgctt
     1741 tttgtctggt agataaaatc agacaatatg aggccatcac cctgccagaa cttataaaaa
     1801 aaatgtttgg ccccagggcc
//
LOCUS       DATDUN010000043         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_43541, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000043 DATDUN010000000
VERSION     DATDUN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43541"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     repeat_region   18..1057
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=18..52
                     /rpt_unit_seq="gtctcaatcccttcacgcagggaactggtctgaat"
     gene            complement(1209..1508)
                     /gene="cas2"
                     /locus_tag="VJC18_00560"
     CDS             complement(1209..1508)
                     /gene="cas2"
                     /locus_tag="VJC18_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HLD43897.1"
                     /translation="MRNPVYNTLAVYDIADVKRLARVAKIVKDYGERVQKSVFELFVS
                     TKELRELHNRVNRVIDVGFDSVRYYFVCETDWQKREYLGVNKFVDEDWNRSFMIV"
     gene            complement(1505..1804)
                     /gene="cas2"
                     /locus_tag="VJC18_00565"
     CDS             complement(1505..1804)
                     /gene="cas2"
                     /locus_tag="VJC18_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HLD43898.1"
                     /translation="MIYVLTYDIAFDSVDDKKAQRRLRKLSKVLENYGWRRQRSIFEF
                     DVDSAVIHKIEVASKKILDLKRDSLRIYPLCVKCLKRSVVQGIGEKIELKDFVIV"
     gene            complement(1801..>2513)
                     /gene="cas1"
                     /locus_tag="VJC18_00570"
     CDS             complement(1801..>2513)
                     /gene="cas1"
                     /locus_tag="VJC18_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HLD43899.1"
                     /translation="LAAKEYFSVFDKGFIKSQGFRVRRRRPPTDPVNAVLSLLYTMLF
                     YRVDNAVERRGLDPYLGHLHAHSYGRRSLVLDLMEEFRPIVVDTLVLSLFNLNIISAS
                     DFEYFQNDEETTDIMSTVKPVTALSQPDVTQDAIGVFSEGRVDTMPREYHAETDVMTS
                     LDDDGHDEATGKRAMVLAKPALKKVLEQFENKLDTEFYHERLSARTTYRKAIDEQVDH
                     YADFLRGDVAVYEPLVMR"
BASE COUNT          729 a          648 c          498 g          638 t
ORIGIN      
        1 cagtgagaat tacgaccgtc tcaatccctt cacgcaggga actggtctga atagccaaaa
       61 aggttaaggc tttcccacag aacaacttgg tctcaatccc ttcacgcagg gaactggtct
      121 gaatacgaaa ataacagaaa aaatggctgt cgtgacagag ggtctcaatc ccttcacgca
      181 gggaactggt ctgaatgaga gtgcaagatg aataacattc taagcgaact gcgtctcaat
      241 cccttcacgc agggaactgg tctgaatcaa tcaagccggg gggaaaacaa aggggaaaaa
      301 aggtgtctca atcccttcac gcagggaact ggtctgaatg ccgtacgtag aggcggaatt
      361 ggccgcgtgg gagacgtctc aatcccttca cgcagggaac tggtctgaat cctatctaga
      421 cacacaaatt gttattatta attacggtct caatcccttc acgcagggaa ctggtctgaa
      481 tcagacgcca aaggggatta ctatattttt gttacgaagg gtctcaatcc cttcacgcag
      541 ggaactggtc tgaataagaa ataaaaaaat attatgaggg tgtcagggca cagtctcaat
      601 cccttcacgc agggaactgg tctgaatgct caaggacggc ccgggattgc agtatctccg
      661 agatcgtctc aatcccttca cgcagggaac tggtctgaat gatgaccctg gacatggatg
      721 gcttaaggtt gaccttgtct caatcccttc acgcagggaa ctggtctgaa ttattgccgg
      781 gataaaacaa aggggaaata ctatgaaggt ctcaatccct tcacgcaggg aactggtctg
      841 aattattatg aaaacgataa ttatttatgg agcgaaacgt ctcaatccct tcacgcaggg
      901 aactggtctg aattcggcaa tgacagtgaa atatctgtta tgaatacggg tctcaatccc
      961 ttcacgcagg gaactggtct gaatccatcg gggtgatcaa cgttacgcaa ccagggcggg
     1021 tggtctcaat cccttcacgc agggaactgg tctgaatagc accatcaaaa tcttcataaa
     1081 tcacgcttcg ttcaacacca aaaccgagaa ccttcaccac ttttttcttc atcatcgttt
     1141 ctttacaaca ggttttcgaa gaaccctgtg aaagtattca taaattttta tgcgagaacc
     1201 atctgaaatc atacaatcat aaaactccta ttccaatcct catccacaaa tttattcaca
     1261 cccaaatact cacgcttctg ccaatctgtc tcacaaacaa aatagtaccg cacagaatca
     1321 aaaccaacat caatcacccg attaactcga ttgtgcagtt cgcgcaattc tttggtgctt
     1381 acaaaaagtt caaacacaga cttttgcacg cgttcgccat aatcttttac aatcttcgct
     1441 acacgcgcaa gcctttttac atctgctata tcgtacactg ccagcgtatt gtaaaccgga
     1501 tttctcatac gatcacaaaa tccttcagtt caattttctc gccaatcccc tgaaccactg
     1561 atcttttaag acacttgacg cacaacggat atatcctcag cgaatctctt ttaagatcca
     1621 atattttttt cgatgcgacc tcgattttat gaatcactgc ggaatcaaca tcaaattcaa
     1681 aaattgatct ctgtcgacgc cagccataat tctccaaaac tttactcagt ttgcgcaaac
     1741 gcctctgagc ttttttgtcg tcaacggaat caaacgcaat atcgtaagtg agcacataaa
     1801 tcatcgcatc accaaaggtt cgtacaccgc aacatcaccc cgcaaaaaat ccgcgtagtg
     1861 gtccacctgt tcatcaatcg ccttccgata agtcgttctg gcagacaaac gttcgtgata
     1921 aaattcagta tccaatttat tttcgaactg ctccaaaact tttttcagag cgggcttggc
     1981 cagcaccatc gctctcttgc cagtggcctc atcgtgcccg tcatcgtcca atgaagtcat
     2041 aacatctgtt tccgcgtgat attcccgcgg catcgtatcg accctgccct cactgaacac
     2101 ccctatcgcg tcctgcgtca catcaggttg ggacagggct gtcacaggtt ttactgttga
     2161 catgatatcg gtagtttctt catcattttg aaaatattca aaatcagacg cactaataat
     2221 gttcagatta aaaagcgaaa gcaccaatgt atcaacaacg atgggcctga attcttccat
     2281 cagatcaagc accaaagacc ttctgccata gctgtgcgca tgcaaatgcc ctaaataggg
     2341 atccagtccc cgccgctcaa ccgcgttgtc tacccgataa aacagcatcg tgtagagcaa
     2401 actcaagacc gcgttgacgg gatctgtcgg tgggcgtcta cgccgcaccc ggaacccctg
     2461 cgatttgata aagcctttat cgaaaacaga aaaatattcc ttggcggcca acc
//
LOCUS       DATDUN010000044         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_2653, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000044 DATDUN010000000
VERSION     DATDUN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VJC18_00575"
     CDS             <1..404
                     /locus_tag="VJC18_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43900.1"
                     /translation="LTVTTEQQLRTDTEPYPWEVFWVFFNYDSEDHQNIRTNYFIFKT
                     NGIELGRFESPDQQTFLATDDEPLVVVGTPNEWVLEKTGQLLSITIDGVLVMTYNGGV
                     ENEALFDHAGSIGLYTEDAQAHVTAIEIQPH"
     gene            549..1616
                     /locus_tag="VJC18_00580"
     CDS             549..1616
                     /locus_tag="VJC18_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HLD43901.1"
                     /translation="MKNMLTAQSTEYKKWLAELKTKVRQVQLRGAIAVNQELLHFYWE
                     LGSDIVQRQKKFSWGEGFLKQLSQDLRSEFFEMKGFSVSNIKYIRQWYLFYFSESSFG
                     QQVVGQIIQIPWGHNLVIIGKCKTVEEALYYVKNTIAYGWSRAVLTHQIESGLWQRDG
                     KAITNFSQTLPAIQSDLAKQTLKDPYVFDFLTLTKEHDERELEQSLIGHITLFLLELG
                     SGFAYVGKQVSLHVGERDFFIDLLFYHTHLHCYIVIELKTGDFEPEHAGKLNFYLKAV
                     DEQLRKPADNASIGILLCKHKDRVVAEYALSDIRKPIGISAYQLTQRLPEELKESLPT
                     IEEIEAELKNAPSDLHTKGEK"
     gene            1792..>2249
                     /locus_tag="VJC18_00585"
     CDS             1792..>2249
                     /locus_tag="VJC18_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HLD43902.1"
                     /translation="MSSVKTTEAFTRVAERFADMQVLRYRIDGFDGLTLSQKTFLYYL
                     QEAALSGRDMVYDQYYKHNLLVRKTIEQIVAHYAGDRHSKDFEKFMTYAKKMWASNGI
                     HNHYTSDKFKPDFSKEYFLSLVEQSSHAEFPWPSGRKGDQTKDLLVSILF"
BASE COUNT          629 a          481 c          536 g          603 t
ORIGIN      
        1 ccctgaccgt gaccacggag caacaactcc ggaccgatac cgagccctac ccgtgggagg
       61 ttttctgggt gttttttaat tacgattcag aagatcatca aaatatccgc accaattatt
      121 ttatcttcaa gaccaacggg attgagttgg gtcggttcga gtctcccgac cagcagacct
      181 ttctggccac ggatgatgag cccctggtgg tggtcggcac gccgaacgaa tgggttctgg
      241 agaaaacagg acaactgctc agcatcacca tcgatggtgt gttggtgatg acatacaacg
      301 gcggtgtcga aaacgaagcc cttttcgatc acgcgggatc gattggtctg tacacagagg
      361 acgcgcaggc gcatgtgacg gccattgaga ttcaacctca ttaataatca ggtcaagagc
      421 cccttttgat tgacattcac aattgatcat ttcatttttt tctgtcattt taaggagagc
      481 tgagattcag aaattaacgg tgattccagc ctgccagccc gcagaccggc tgataaaaaa
      541 agttgcttat gaaaaacatg ttaacagctc aatcgacaga atacaaaaag tggcttgctg
      601 agctcaaaac caaggtgagg caggttcagt tacgcggagc aatagctgtt aaccaagagt
      661 tgttacattt ttactgggaa ctcgggtctg atattgttca aagacaaaaa aaattttctt
      721 ggggagaggg ctttcttaaa caattgagtc aagatttgcg gagtgaattt ttcgagatga
      781 aagggttttc ggttagtaat atcaaataca tacgtcagtg gtatttgttt tatttctctg
      841 aaagttcatt tggccaacag gttgttggcc aaattattca gattccttgg ggtcacaatt
      901 tggtgatcat tggcaagtgc aaaaccgtgg aagaggctct ctattatgtt aaaaatacga
      961 ttgcctatgg ttggagtcgt gctgttttga cccaccaaat tgaaagcggc ctttggcaga
     1021 gagacggcaa ggccatcaca aatttttccc aaacacttcc tgcgattcag tctgatttgg
     1081 ccaaacaaac gttgaaggat ccttacgttt ttgattttct cactctcacc aaagagcacg
     1141 acgaaaggga attggaacaa agcctcattg ggcatattac attgtttctt ttggaattgg
     1201 gatcaggctt tgcgtacgtg ggtaagcagg tttcattgca tgtgggagag cgtgactttt
     1261 ttattgatct tcttttttat cacacgcacc tgcattgtta cattgtcatc gagttaaaga
     1321 cgggtgactt tgagcctgag catgctggta aacttaattt ttatctcaag gcggtggatg
     1381 agcaactgcg gaaacccgca gataatgctt ccattggaat tttgttgtgc aaacataaag
     1441 accgtgttgt ggccgaatac gccctcagtg atattcgcaa acctataggt atctctgcct
     1501 atcagttgac tcaacgactt cctgaagagt tgaaagaaag tctgccgacc attgaagaaa
     1561 tagaggccga actcaaaaat gccccttctg atctgcacac aaagggagag aaatgagggt
     1621 gagcggaaga aaaacaagct atcgagcaga gcagtaaact ttatgcaaat aaaaagtcat
     1681 tactatctga ttttgtttgt cctgataggt gtgtcgctgg ttgtgtggca aatgaattgg
     1741 aaaacagcta accagccgac acagcaaagc accattttac cggaggtccc catgtcatca
     1801 gtaaaaacca ccgaagcctt tacccgcgtg gccgaacgtt tcgccgatat gcaggtcttg
     1861 cgctaccgta tcgacggctt tgacgggctg acgctgagcc agaaaacatt tttgtactac
     1921 ctgcaggagg cggccctgtc cggtcgcgac atggtgtatg accaatatta caagcacaat
     1981 ttgctggtgc gtaaaaccat cgaacagatt gtggcgcact atgcaggtga tcgccatagc
     2041 aaagattttg aaaagttcat gacctatgct aaaaagatgt gggcttcaaa tggcattcac
     2101 aatcattaca ccagtgacaa attcaaaccg gatttttcaa aggaatattt tttatcactc
     2161 gttgaacagt cctcgcatgc ggaatttccc tggcccagcg ggaggaaggg cgaccagacc
     2221 aaagacctcc tggtctccat cttgttcga
//
LOCUS       DATDUN010000045         6181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_375761, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000045 DATDUN010000000
VERSION     DATDUN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375761"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1238
                     /locus_tag="VJC18_00590"
     CDS             207..1238
                     /locus_tag="VJC18_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLD43903.1"
                     /translation="MFSPQYTISDQMVTTLAEIEKLTLQLMQTSIPSEIFEHIQQQCL
                     VALTHFSTQIEGNQLSLEQVSGVIEKRKTFGFVRDEKEAKNYFTLLERMTPLIKKHQR
                     KLDAKLILECHSQLLSRIVTESSRGKFRTVQNAIYETGSKKNVCLPPEAKDVMLLIDE
                     LCYWVNVTKAHPIICAAIFHNQFVTVHPFIDGNGRSARFLSLYLLETRNYDWKKIVPI
                     DRYYADNRPLYYDQLQQHYSHNYYFGRHNTDFTKWLEYYVEGIKQMLLGTLNQIALFR
                     TQNILLNNRQNKMLKYLQEVSAISVSEYARRFGISTRMASRDLAQLLELNRVGVMGKA
                     RATKYFLKP"
     gene            complement(1270..2262)
                     /locus_tag="VJC18_00595"
     CDS             complement(1270..2262)
                     /locus_tag="VJC18_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HLD43904.1"
                     /translation="MIIGLTGKNGSGKGVVAEYLKNAGYIYHSLSDIIRLEIQREGHE
                     VTRERLIDMGRKLREEGGPSVLADKVLALLDIDKNYVIDSIRNPFEVRALKYRKDFFL
                     FCVTADQKTRFERTKERARENDPVDFKKFVELEEKELHSTDPSAQQLNATAALADFEI
                     ENNGSVQDAQDKVKDIMVTLLKKNSRPDWDTYFMNIARNVSLRSNCLKRKVAAIIVKD
                     KRIISTGYNGTPRGVKNCDEGGCPRCNSFAPSGTALGDCLCSHAEENAIVQAAYHGVS
                     IKDATLYCTFSPCLGCTKMILNSGIKEVVYNANYPLGEVALGLLQGADIILRKM"
     gene            complement(2373..3911)
                     /locus_tag="VJC18_00600"
     CDS             complement(2373..3911)
                     /locus_tag="VJC18_00600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLD43905.1"
                     /translation="MNLIRDNDEKYYWFFLFILTLLLRSASFYTPVLDVDETQFAGFA
                     HVLMDGGLPYVDSLDTKPLGIYLFYWFVFALFGRTNMVAVHVVTACISFATAYFLYLV
                     FAQWERKREGRWAALLFVVFSTTFVPKYIATSINSVMVFFLVLSVYFMARSARSGVLL
                     DSALSGLILGLALLFKYTAGIQCVLFFCFSGIWMLLSRVDRSRAQVLKTFIVHNAIFA
                     LAFVLPFVLHGVVLWHIGVWPDFVNWSLAGSGAYIAQGGSTIAFWQSLLIRFGGYVLA
                     TMFVWIYSVRGFQKSLLRHPLYLLLMCWFVLTLVPVCLGGRFYPHYFIPLLPSLCGLA
                     AYGVNRVKVSKTVVVLLIVLPATLFWTLRLDYRTFLKHFPDDAIYEQQSVGERIKVMS
                     QPGDTLFVWGFATGIYFSSGLKPASRFLWTDLLTGRTPGPYYARRNQDQEGTYANEGA
                     WRVFWQDVEKSHPTFFVDTSPANIHDYRRFPISRYPALQAFIQKNYEKVDNVEGVGVY
                     RARE"
     gene            4075..6135
                     /locus_tag="VJC18_00605"
     CDS             4075..6135
                     /locus_tag="VJC18_00605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLD43906.1"
                     /translation="MREQTLNIRTCDDYMPQPQTKQAFQKLGRYLIVGEIAKGGMATI
                     YRAKLIGVEGFEKDVAIKKILPYWSDQQEFVDMLIDEAKILVRLQHNNIVQIFELAKE
                     KDTYFIVMEFVKGFDLKKIISKLKKAQKPFPLNLACYIIKEICKGLDFAHSRKGSDSH
                     PLNIVHRDISPQNIMVNTDGNVKITDFGIAKIMGRSTETAAGALKGKFSYMSPEQALG
                     RDINSLTDIFALGALFYELVFLTKCFDGKNDLEIIEKVKDANIDLPAGCDPNLAGILL
                     KAMSREADHRYQNTMLLRSDIRELEKTQRCHADQDDLKNFLAVLFEDEITLVEQQENK
                     IEEKTKIFSDPQRASASVVTKINQNNASVGQDPSVGKSENSSLTIIEADTIVDSKILE
                     YAALSRSVTPSAPVRPTVSDAVAKSPLPLIVQLHNPLDRVLVIKKTRSSLSGFAFLRK
                     TGTLIGQHPFLSLGILGFCLLLSLLTFLSGNKSLQDTTPKPTAVSVPQTPELIRNRVD
                     TLELFDTPRKELSPEEIAPKQTTTLMARVTITAEPPSSLIEITQGKQTLTFTGFASHE
                     FAFLETDTPLSARVSHEGYATQEMILPVRKEQLIIKQEISLKPLSYGRVSVTARPWGT
                     AKIDGQAHATPFVFTVPSGQHVVSVYFPAQKKTISQTTNVSPGKQTNCEASLRDMNSR
                     IKCN"
BASE COUNT         1792 a         1638 c         1319 g         1432 t
ORIGIN      
        1 ccgtccacgg gaattttttg tcctacacca tcgttgacat accgcataaa gacctctctt
       61 tttttgtgtg acagaccggc cccccgcaag caaactctta gcaatttgac aagcgttaac
      121 gcaaacagaa aatacagcaa aacgaccagg ttttgtgtct tgattatgtc ttgattgtgt
      181 cttgattctg gattacaata cctcgaatgt tttcaccaca atacaccata tcagatcaga
      241 tggttacgac tctggcagaa atagaaaaac tgaccctcca gttgatgcag acatccattc
      301 caagcgaaat ttttgaacac atccaacaac aatgcctggt tgccctcacg catttctcaa
      361 cccaaattga aggcaatcaa ctgtcgctgg aacaggtttc tggcgtgatc gaaaaacgca
      421 aaacatttgg ttttgtgcga gacgaaaaag aagcgaagaa ttattttaca ctgcttgaaa
      481 gaatgacccc gctgatcaaa aaacatcaaa gaaaattgga tgccaaattg attctggagt
      541 gtcactcaca gttgctgtcg cgaattgtga cagagtcatc gcgagggaaa ttcagaactg
      601 tccagaacgc gatttatgaa actggttcga aaaaaaatgt ctgtctcccg cccgaagcca
      661 aagatgtcat gttgctaata gacgaacttt gttattgggt taatgtcacg aaagctcatc
      721 ccatcatttg cgcggctatt tttcataatc agtttgtgac cgttcatccc ttcattgatg
      781 gtaacgggcg ctcagccagg tttttaagcc tctatttact ggaaaccagg aactatgact
      841 ggaaaaagat tgttcccatt gatcggtact atgccgataa tcgcccgttg tattatgacc
      901 aattacaaca gcattattca cacaattatt attttgggcg acacaacact gattttacaa
      961 agtggctaga atactatgtc gaaggcatta aacaaatgct gttgggcaca ttaaaccaga
     1021 tagcgctttt cagaacacaa aatattcttc tcaataatcg ccaaaataaa atgctgaaat
     1081 atcttcagga ggtttctgct atttcggtat cggaatatgc cagaagattt ggtatttcca
     1141 cacgcatggc ttccagagat ctggcacaac tgctggaatt gaatcgcgtg ggcgtaatgg
     1201 gaaaagcccg ggctacaaaa tattttttaa agccgtgagg agagacattc tgtcgcctgc
     1261 ccctatgatt cacatctttc gcaagatgat gtcggcaccc tgtaaaagac ccagagccac
     1321 ctcacccaac gggtagttcg cgttgtacac cacttccttg atgccggaat tgagaatcat
     1381 tttggtgcaa cccaaacacg gcgaaaacgt gcaatacagc gtggcgtcct tgatggacac
     1441 gccatgatac gcggcctgca caatggcatt ctcttccgca tgtgaacaca agcagtcccc
     1501 caaggccgtc ccgcttggcg caaaactgtt gcaacgggga caccccccct catcacagtt
     1561 tttcacacct cgcggcgttc cgttgtatcc ggtcgaaatg atgcgcttgt ctttgacgat
     1621 gatggccgcc accttgcgct tgaggcaatt gctccgcaga ctcacattcc gcgcgatgtt
     1681 catgaaatag gtgtcccagt caggtcggga gttttttttt aagagtgtga ccatgatgtc
     1741 tttgactttg tcctgcgcgt cctgcacact gccgttgttc tcaatttcaa aatcggccaa
     1801 agccgcggtg gcattgagtt gctgggcaga gggatcggtg ctgtgcaatt ccttttcctc
     1861 cagttccaca aactttttga aatcaacggg gtcgttttcg cgcgctcgct ctttggtacg
     1921 ctcaaaacgc gtcttctgat ccgccgtcac gcaaaacaga aaaaaatcct tgcgatactt
     1981 gagagcccga acctcaaacg gattacgaat ggaatcgatc acgtagtttt tgtcgatgtc
     2041 cagaagggca agcactttgt cggccaacac agacgggcca ccctcttcgc gaagcttgcg
     2101 tcccatatca atcaggcgtt cgcgggtcac ctcgtgacct tcgcgctgga tttccagacg
     2161 gatgatgtct gagagtgaat gatagatgta gccggcgttt ttgagatact ccgccaccac
     2221 acccttacct gaaccatttt tgcctgtgag tccgatgatc ataattcgtg gatcgatggt
     2281 cgtggatcga aattcactcc cttttgcacc ttgccttttg catgatgcct ttcttcgtcc
     2341 ctcgtccctt cgtccctcgt gttttcgtta ccctattccc ttgccctata tacccccacc
     2401 ccttccacgt tatcaacctt ctcgtaattc ttttggataa aggcctgcaa ggcaggataa
     2461 cggctgatcg gaaaacgcct gtaatcatgt atgttggccg ggctggtgtc tacaaaaaaa
     2521 gtggggtgag atttttctac atcctgccaa aaaaccctcc aagccccctc gtttgcataa
     2581 gtcccttcct gatcctgatt cctgcgggca tagtaagggc ccggggtgcg ccctgtcagc
     2641 aaatccgtcc acaaaaaccg acttgccggt tttaaaccag aagaaaaata aatccccgtc
     2701 gcaaaccccc acacaaatag tgtgtcgcca ggttgactca tcaccttaat ccgttctccc
     2761 acgctttgct gttcatagat agcatcatcg ggaaagtgtt tgagaaatgt gcgataatcc
     2821 agcctgagtg tccaaaacaa tgtcgcaggc aaaacaatca acaaaacaac aaccgttttg
     2881 gacaccttaa cccgattcac accataagcc gccagcccgc acaaggaagg cagcagagga
     2941 ataaaataat gtggataaaa gcgccccccc aaacagacag gaaccagcgt caacacgaac
     3001 cagcacatga gtaacaaata caaagggtgc ctcagcaaag atttttggaa ccccctgact
     3061 gaataaatcc agacaaacat ggtggccagc acataaccac caaaacggat caacagactt
     3121 tgccaaaaag cgatggttga gccgccctgt gcaatgtagg caccactccc cgctagtgac
     3181 cagttgacaa agtcgggcca gacaccgatg tgccacaaca caaccccatg caagacaaag
     3241 ggcaaaacaa aggccagggc aaagatagcg ttgtgcacga taaaggtttt cagcacctga
     3301 gcgcgtgaac gatcgacccg ggacagtagc atccaaattc cggagaaaca aaaaaacaga
     3361 acacactgaa tccctgccgt gtacttgaac aataaggcca aacccaaaat cagtccggac
     3421 aaggcagaat caagcaggac gcctgaccgc gctgatcgcg ccatgaaata aaccgacagc
     3481 accaaaaaaa acaccatcac gctattgatc gatgtggcaa tgtacttggg cacaaacgtg
     3541 gtggaaaaca ccacaaacaa caatgccgcc cagcgtcctt cgcgctttct ctcccactga
     3601 gcaaaaacca gatataagaa atacgcggtg gcaaaactga tacaggccgt caccacatgc
     3661 acggctacca tattggtcct gccaaaaagg gcgaacacga accaataaaa cagataaatc
     3721 cccaacggct tggtgtcgag gctgtccaca tacggcagac caccatccat gagcacatgg
     3781 gcaaaacctg caaattgggt ttcatcaaca tccagaacag gtgtgtaaaa agaggcgctc
     3841 ctcaacaaca aagtgagaat gaataaaaaa aaccagtaat atttttcgtc attgtccctt
     3901 attaaattca tcgtcctgtc caaaaacaca aacactttgt tctaaaaata cgacgcctgt
     3961 cgccgtttaa ggacaatccc cgtaaaaggc cagaaataaa aaactcacaa caccattcac
     4021 ttacgaaaca ttgtcagttt agcacagttc ttgcattcat tgggaatcgt cactatgaga
     4081 gaacaaaccc tcaatatcag gacctgtgac gattatatgc cacaaccgca aacgaaacag
     4141 gcgtttcaga agcttggtcg ctacctcatc gtaggcgaga ttgccaaggg cgggatggcc
     4201 accatctacc gggccaaact cattggcgtg gaaggttttg agaaagacgt ggccatcaaa
     4261 aaaatcctgc cctactggtc ggaccagcag gaatttgtag acatgctcat cgatgaggcc
     4321 aagattctgg tgcggcttca gcacaacaac attgttcaga tttttgagtt ggccaaggaa
     4381 aaagacactt acttcattgt catggagttc gtaaaagggt tcgatctcaa aaaaatcatc
     4441 agcaaactca aaaaggccca gaaacccttt cccctgaatc tagcctgtta catcatcaag
     4501 gaaatctgca aggggttgga ttttgcccat tcacgcaagg ggtccgacag ccacccgcta
     4561 aacatcgtgc atcgtgacat ctctccccaa aatattatgg tcaacaccga tggcaatgtg
     4621 aaaatcacgg acttcggcat tgccaagatc atgggcagat ccaccgaaac cgccgccggc
     4681 gccctcaagg gcaagttcag ctacatgtct cccgaacagg ccttggggcg cgacatcaat
     4741 tccctgaccg atatctttgc cctgggcgcc ctgttttacg agctggtttt tctgacgaaa
     4801 tgtttcgacg gcaagaatga tctggaaatc atcgaaaagg tcaaggacgc caacattgat
     4861 ctgcctgcag gctgcgaccc gaatctcgcc ggtattttgc tcaaagccat gtccagagag
     4921 gctgatcacc ggtaccaaaa caccatgttg ctgagaagcg acatccgcga attagaaaaa
     4981 acccaacggt gccacgccga tcaggatgat ctaaaaaact ttctggcggt tcttttcgaa
     5041 gacgaaatca ctttggtgga acaacaagaa aacaaaatcg aagaaaaaac aaaaatattt
     5101 tccgatccac aacgagctag tgccagtgtc gtcaccaaga ttaatcaaaa taacgcgtcg
     5161 gtagggcaag acccctcagt cggcaaatca gaaaacagct ctctgaccat cattgaagcc
     5221 gacacgattg tagacagcaa gattcttgaa tacgccgccc tctctcgttc tgtcacccct
     5281 tctgcgccgg tcagaccaac tgtttcggat gctgtcgcca agtcccccct ccccctgatc
     5341 gtgcaacttc acaaccctct tgatcgggtc ctggtcataa aaaaaacacg ctcctcgttg
     5401 tctgggttcg cgtttctcag aaaaacggga acgctcattg gccaacaccc ttttctctcg
     5461 ttgggaattt tgggtttctg tctgctgctt tcattactca cttttctatc ggggaacaaa
     5521 tcgttacagg acacaacacc gaaaccgacc gcggtttcag tcccacaaac accagaactc
     5581 atacgaaatc gtgttgacac gcttgagctt ttcgacacac caagaaaaga attgtcacca
     5641 gaagaaatcg cccccaaaca aaccaccacc ttaatggcca gggtgaccat aacggcagag
     5701 cctccttcat cgctcatcga gatcacgcag gggaaacaaa cgctgacctt cacgggattt
     5761 gcctcgcacg aattcgcctt tttggaaact gacacgcccc tttccgcgag agtttcccat
     5821 gaaggttatg ccacacagga aatgatcctc cctgtcagga aagaacaact catcatcaaa
     5881 caagagattt cccttaaacc gctcagttat ggccgcgtga gtgtgacagc caggccgtgg
     5941 ggaaccgcga aaatagacgg gcaggcacac gccacaccct tcgtttttac cgtcccatcg
     6001 ggacaacatg ttgtgagtgt ttattttccg gcccagaaaa agaccatcag tcaaacaaca
     6061 aatgtctcgc ctggaaaaca gacaaattgc gaggcctctc taagagacat gaacagccgt
     6121 atcaagtgca attgagaact attagaattt aaaacagtct ttgcccgcaa atttggcctc
     6181 g
//
LOCUS       DATDUN010000046         1060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_583734, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000046 DATDUN010000000
VERSION     DATDUN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583734"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1060)
                     /locus_tag="VJC18_00610"
     CDS             complement(<1..>1060)
                     /locus_tag="VJC18_00610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLD43907.1"
                     /translation="IWTLLNYLDPEKYPSRQAFKEMFTTTTGGLLLLHGELTDMMLRR
                     TKQETMTHFTNPAESPFAEQLADGVPRLPKKIRVTPEVSGAYALAPEQADLIAWMIAD
                     YRGWINYFNENLPDGAERIESDAVNSLHKFQMIHKVIYEPEYFGLDCENPLYPALDQM
                     VARHLAKDEKVILWCWNTELITTLQKRYAQFGVSRIDGSVMGEAREKARHEFQNDPHV
                     RIMDANYLSGGVGLTLTAAHSAIFVQLPHVFPPLYQAEGRHHRLIGPHKIQFAKEHVF
                     VEWMVPTYHAGFVDSIDDERLMEKLTHGTLVEQTRRRLEGGELLYNLVMEGYGDLEDL
                     EEYFKLGVMEAMGLNDAEA"
BASE COUNT          266 a          268 c          238 g          288 t
ORIGIN      
        1 gcttctgcgt cattaagccc catagcctcc ataacaccca atttaaaata ctcttccaga
       61 tcttccaaat caccataacc ttccatcacg agattataca gaagttcccc accttccaaa
      121 cggcgtctcg tttgctctac aagcgtgccg tgcgttaatt tttccatcaa acgttcatca
      181 tcaatgctat caacgaatcc cgcatgataa gtgggcacca tccactcaac aaaaacatgc
      241 tcttttgcaa attgtatttt atgtgggcca atgaggcgat gatgtctgcc ttctgcctga
      301 tacaggggag gaaagacatg cggcaattgc acaaagatgg cagaatgtgc cgctgtgagt
      361 gtgagcccga cgcctcccga gagatagttg gcatccatga tgcgaacgtg agggtcattc
      421 tgaaattcat gtctggcttt ttctctggct tcccccatga cactgccatc aatacgactc
      481 acaccaaact gggcatagcg tttttgcaag gttgtgatga gttcggtgtt ccaacaccac
      541 agaatcactt tttcgtcttt ggcaagatgt ctggcgacca tttgatcgag cgctgggtac
      601 aagggatttt cacaatccag tccgaaatat tctggttcat agatcacctt gtgaatcatt
      661 tgaaatttgt gcagggaatt gacggcatcg ctttcaatac gctcagcccc atcaggcaga
      721 ttttcattaa aataattgat ccaaccacga tagtcagcga tcatccaggc gatcagatca
      781 gcctgttctg gcgccagcgc gtacgcacca ctcacttctg gggtaacgcg tattttttta
      841 ggcagtcggg gcacaccatc ggcgagctgt tcggcaaaag gactttcagc agggtttgta
      901 aaatgggtca tggtttcctg cttggtcctg cgcaacatca tgtctgtgag ttcgccatgt
      961 aacagcaaca acccacctgt ggttgtggtg aacatttcct taaaggcctg gcggctcggg
     1021 tatttttcgg gatccaggta atttaaaagg gtccagatac
//
LOCUS       DATDUN010000047         1883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_458817, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000047 DATDUN010000000
VERSION     DATDUN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458817"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VJC18_00615"
     CDS             <1..452
                     /locus_tag="VJC18_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43908.1"
                     /translation="LMGSVATFALGTTPEHRIIAGGLGTGGLAFAINGLMGTIMRAQY
                     GTIACCMLDANGRLSQRDIMSLLLFNVAERETFIRFLQDAPTQFPDLIKKYPDTLQAV
                     ENKLSIFIKGVGCPTSQRQRKQLIQLGDICSLINQLTDHNSVTDSEQ"
     gene            complement(487..1206)
                     /locus_tag="VJC18_00620"
     CDS             complement(487..1206)
                     /locus_tag="VJC18_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43909.1"
                     /translation="MIGSDFGKCIPDKAWQADRHQRRTRLIPQTPVTIKNKDGSFQTL
                     QFADYNEKLDFHHKLAFVVDGHAQIFSRRNEHDVHLSDDSVVAIDAIDPTEFQYIVDD
                     FDGDGDDDVCVAFATEQKWNHEQVELMTFENDGYGKFARVGFNPKKNAWGIASARCYN
                     GMCFWEDAPKTSDISNLRLKKKPETDKAFEVSFDVSDKSSTTNAFSLFAASSQIDSST
                     FVVDHQMSADFLVFMASYMDR"
BASE COUNT          519 a          445 c          432 g          487 t
ORIGIN      
        1 tactaatggg aagtgtcgcc acctttgcgc tcggaacaac acctgaacac aggatcatag
       61 ctggcggtct gggcacaggc gggctcgcct tcgccatcaa cggtttaatg ggcaccatca
      121 tgcgcgccca gtacggaacc attgcctgtt gtatgctgga tgccaatggc cgattgtcgc
      181 aacgggacat tatgtcactg ttgctcttca acgtcgcgga acgtgagacc ttcattcgtt
      241 ttttacagga cgcccccaca caatttcccg atctcatcaa gaaatatccg gacacattgc
      301 aagcggtaga aaacaaatta tcgatattta tcaaaggggt aggttgtcca acctctcaga
      361 ggcaacgaaa gcagctaata caattaggag atatctgctc gcttattaac caacttacag
      421 atcacaactc cgttacagat tccgaacaat gacactcctg acgcttgatc agggcaagac
      481 ccaccatcat cgatccatgt aactggccat gaagaccaga aaatccgccg acatttgatg
      541 gtccactaca aaagtgctgg aatcgatttg ggaagatgcc gcaaagagcg aaaacgcatt
      601 tgttgtgctt gatttgtcgc tgacatcaaa cgagacttca aaggctttgt ccgtttctgg
      661 tttttttttg agcctaagat tggaaatgtc cgacgtcttt ggggcatcct cccaaaaaca
      721 catgccattg tagcaccggg cagaggcaat gccccatgca ttttttttgg ggttaaaccc
      781 cacgcgggca aattttccat aaccatcatt ttcaaaagtc atcaactcca cctgctcgtg
      841 gttccacttc tgctcggttg caaaggccac acacacatcg tcgtcaccat caccatcaaa
      901 atcatccaca atgtattgaa actctgttgg gtcaatcgca tctatggcca caacgctatc
      961 gtcagaaagg tggacatcat gctcgtttcg ccgtgagaat atttgcgcgt gcccatccac
     1021 aacaaaggct agtttatgat gaaaatcgag tttttcgtta taatcggcaa actgcaaagt
     1081 ctggaatgaa ccatccttgt tttttatggt cacgggagtc tgtggaataa gccgtgtgcg
     1141 ccgctggtga cggtctgcct gccaggcctt atcgggaatg cactttccaa aatcgctacc
     1201 tatcataagg ggggtccttt ctcgggatgg gaaatcagag gtgaataatc tacaagttac
     1261 aaatgcaaaa ccaatgccaa gaatgttaat tattattgat tgaaatatca ggttgttaat
     1321 cagagacaat gcttgttatg tgttgccagt tggtgtgaag agcccccaag gtctgtattt
     1381 tttgaggaat gaatacagca ggaatgattg aagagtattt tggtgagtga tagaatcaaa
     1441 tacctgaaca tttcgaacgg gtgactaaga aaacctgctg agataatttt tttgggtgat
     1501 acgggaagct attattttga tgcggcctgc ggtgtagggg ccgggcttga ccggcccaac
     1561 tatcgggcga gtctagcctc gcccctacac attgttcgat gtcttcaaaa aatagcgacc
     1621 cgtagcagcc tgcccgccga agctttagcg taggcaggga cccaaaaaaa tagcaaccaa
     1681 gaggttttcg aatagaatat ctaaaggagg gttcatgcct aaaatgattt ttcccatcat
     1741 cggtttggtg gcggcaatgg gcgtttttgt ctacacactt gtcagggcca aaataaatct
     1801 taaactcacc cacctcatca aggacacccc cacatccaaa attggtttca tcaaaaatgg
     1861 gtttgtggag gtctgcggca caa
//
LOCUS       DATDUN010000048         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_126592, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000048 DATDUN010000000
VERSION     DATDUN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126592"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VJC18_00625"
     CDS             complement(<1..413)
                     /locus_tag="VJC18_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HLD43910.1"
                     /translation="MLPLNISPAVKNAFQKNIPVVALESSLLCQGMPYPQNVQAMLQV
                     EQIIREQGCVPATIAIKKGELLIGLSGEEILYFGQRGLDCVKVSRRDLSFVISQKLDG
                     ATTVASTMFLAQKAGIQVFATGGIGGVHRGASETQ"
     gene            complement(464..1111)
                     /locus_tag="VJC18_00630"
     CDS             complement(464..1111)
                     /locus_tag="VJC18_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43911.1"
                     /translation="MQLIELIQARIESLYGIKIGAKAGDYLIGEDELFELLPAHQQTV
                     VPKELFLVNPDPDNDTVEVALFLDSKLKENLSHRNPLERLCAENIGDFCALIEGISHF
                     VCYLHKAHLNREITQLELELQAEIDKFLLLSLLTYSDEANSYQLIDVLFEGYAINEAL
                     ALDQIERYETASTLAKKYCASLACDIKARRIKNLVKKIREFYSLSQEEKIRMIVC"
     gene            complement(1145..1756)
                     /locus_tag="VJC18_00635"
     CDS             complement(1145..1756)
                     /locus_tag="VJC18_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43912.1"
                     /translation="MDKKLTTSHDIREFFYKKVKEAIMIQDVRVSEDVEVYLVHVLAY
                     FSKSENLFREDEEGRLEYRALALKLYDAVFSSPNERVQHLRSLGDTALYHAGVFYEGL
                     LSGVVDVNYYISMGGTAYLSLANMSTHVGSHAAKMYHQMGQQFKDLVTLLNVCCEKEG
                     TPTDIDLIKILDRYLRTGSKRDKEILTKAGIVPEGLGINTTEQ"
     gene            complement(1984..>2475)
                     /locus_tag="VJC18_00640"
     CDS             complement(1984..>2475)
                     /locus_tag="VJC18_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43913.1"
                     /translation="LFWKNADRPVIRKLARFRLVGWLLRHNLFLIFREAKLGDYLLLF
                     VLRIPLIIAVIGGYNLALGTFGFAIPWSKIFLYNPIIMLIATLPITPAGLGTGQFFTI
                     QFFQNHVSHSNLLIDPVSAASLLLASSLIWFLANQIIKVIFGVVCLIWTNSAQFKISD
                     RAQ"
BASE COUNT          687 a          627 c          544 g          617 t
ORIGIN      
        1 tcctgtgtct ccgacgctcc ccggtgcacc cccccaatgc cccccgtggc aaaaacctga
       61 atccccgcct tttgcgccaa aaacatggtg ctggccaccg ttgtggcccc atccaacttc
      121 tggctgataa cgaaagagag gtctcggcgc gacaccttga cacagtccag gcccctttgt
      181 ccgaaataca aaatttcctc tccggacaaa ccaatcagca gttcaccctt cttgatcgcg
      241 atggtcgcgg gcacacagcc ctgttcacga atgatctgct caacctgcaa catcgcctgc
      301 acgttttgag gataaggcat tccctgacac agcagggatg attcaagggc aacaacggga
      361 atgttttttt gaaacgcgtt tttgacggct ggggaaatat ttaaaggcaa catggtccgt
      421 ggttagcggt tttgggaggc aaaagcaaga atgtttgggg cgctcaacaa acaatcattc
      481 tgatcttttc ttcctgggag agggagtaaa actcgcggat ttttttgaca agatttttga
      541 tgcgtctggc cttgatgtca caagccagac tcgcacaata ttttttggcc agcgtcgagg
      601 ccgtttcgta ccgctcaatc tgatcaagcg ccaaggcctc gttgatcgca taaccctcaa
      661 acaacacatc gatcagctga tagctgttgg cttcatctga gtaggtgagc agagacaaca
      721 acagaaattt atcgatttcc gcctgcagtt ccagctcaag ctgggtgatt tccctgttca
      781 gatgcgcctt gtgcaaataa caaacaaaat gcgaaatccc ctcaatgagg gcgcaaaaat
      841 cgccaatgtt ttccgcgcac agcctctcca aaggattgcg gtgggacaaa ttctccttga
      901 gtttgctgtc cagaaacagg gccacttcga ccgtatcgtt gtcgggatcg gggttgacca
      961 aaaaaagttc cttgggaaca accgtttgtt gatgggctgg cagaagctca aaaagttcat
     1021 cctcgccaat gaggtaatca cctgccttgg cgccaatttt gataccatat agcgactcaa
     1081 tccgcgcttg tatgagttca atcagttgca tagtccaagt tgcctgacat caatgaaaaa
     1141 cgatttattg ttccgtcgtg tttattccca gtccctcggg aacaatcccc gccttggtca
     1201 atatttcttt gtctctcttc gagcctgtcc ggaggtagcg atccagaatt ttgatcaggt
     1261 caatatctgt gggggtccct tctttctcac aacaaacatt caacagcgtc accaaatcct
     1321 taaattgttg tcccatctga tgatacattt tggccgcgtg cgaaccaaca tgagtcgaca
     1381 tgttggccaa agacaaatac gcggtacctc ccatgctgat gtaataattc acatcgacca
     1441 caccgcttaa caacccttca taaaacacgc ccgcatgata caaggcggtg tcgcccaaag
     1501 aacgcaaatg ctgtactctc tcgttcggac tggaaaaaac ggcatcatat aatttgagcg
     1561 ccaacgcgcg atattccaac cgcccctctt catcctcccg aaataaattt tctgattttg
     1621 aaaaataggc gaggacatgc accaaataca cctcgacatc ctcagaaacc cggacgtctt
     1681 gtatcatgat ggcttccttg acttttttat aaaaaaactc tctgatgtcg tgactcgtcg
     1741 tcagtttttt atccatcaac tattattgta acagatttta gaaaggtgtc taatgatttt
     1801 ggagaatata ttttgtcatt ccagcgaatg ctggaatcca tgtttttttc acaggatccc
     1861 tgcctacgca gggatgacag acatcactcc ccctgttcac tacatggcct gagcacaata
     1921 ggagtcttcg ctattcaggg ggtagctatg tgttacaggc gttgattttg agcaacagac
     1981 ttttcactgt gctcgatcag agattttaaa ctgtgcagaa ttggtccaaa tgagacacac
     2041 gacaccaaag atgaccttga tgatctggtt ggccagaaac cagatgagac tggaggccaa
     2101 aagaagtgac gccgcagaca cagggtctat gagcaaattg gaatgactca catgattttg
     2161 aaaaaactgg atcgtaaaaa attgccctgt ccccaaaccc gcaggcgtga tgggcagggt
     2221 cgcaatgagc atgatgatgg gattgtataa aaaaatcttt gaccagggaa tcgcaaaacc
     2281 aaaggtacct aacgcaagat tatagccccc gatcacggca atgatcagag ggatacgaag
     2341 gacaaacagc aacagataat cccccagttt ggcttcacga aaaatcaaaa acagattgtg
     2401 cctgagtaac cagccaacca aacgaaaccg cgccagtttg cggataacag gacgatcagc
     2461 gtttttccaa aacaa
//
LOCUS       DATDUN010000049         3584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_583767, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000049 DATDUN010000000
VERSION     DATDUN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583767"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VJC18_00645"
     CDS             complement(<1..617)
                     /locus_tag="VJC18_00645"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HLD43914.1"
                     /translation="MIIACLKEVKTREGRVCLIPDNVKQLKAGGHTVLIQKNAGVLSG
                     FEDRDYVEAGAEIVEKTKELIERSDLIVKVKEPTIEEINMMHEGQIFFGYLHLAPIPD
                     TMRAILNSKIHALGFETLQLEDGSLPLLIPMSEIAGKLATQNGAHFLRFDQGGRGVLM
                     GGTRTVKPANVLILGGGVVGRCAADVAIGMGGNTTILDISKKKLD"
     gene            799..1452
                     /locus_tag="VJC18_00650"
     CDS             799..1452
                     /locus_tag="VJC18_00650"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLD43915.1"
                     /translation="MTEKTPNTVSVFNPATYLTISRFFLVPVFIYFFLVAKFAIALVI
                     LVIASITDITDGLLARRFNMGTKIGSVLDPLADKFLMLISFLVLSTQTILPWQLATIV
                     IGRDFYIVFGVMYLYFVKKLKIHFQPTMLSKRTTFFQFMCLAFSFLKVYLTATPQALT
                     QVYTIYSYKCNLRILALSMHDVMVLVAGLFTAATFIQYTIMGLQILKRNEKREPQMI"
     gene            1555..1899
                     /locus_tag="VJC18_00655"
     CDS             1555..1899
                     /locus_tag="VJC18_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HLD43916.1"
                     /translation="MHELSIATSIVEIVENNLKTYPTGKARSVTVQLGTHCGVEPSAL
                     EFCFPIAIEGTRVQGAQLILQILPLTLNCSSCLAQNVTASALQCPHCHSPEVTVVSGR
                     DMIVTSIDLDVK"
     gene            1911..2564
                     /gene="hypB"
                     /locus_tag="VJC18_00660"
     CDS             1911..2564
                     /gene="hypB"
                     /locus_tag="VJC18_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HLD43917.1"
                     /translation="MTTLNIKSNVLSKNDQVAQEIKKIFAKNNITALNLIGSPGSGKT
                     ALLEATFKKLRGRLDCAVIEGDVKTDNDKKRIEATGVPAVQIETEGGCHLNAEQILKA
                     LEAFRLAEYHCIIIENVGNLICPTSYDLGERYRVIVISVGEGVDKPLKYPSAFTSANV
                     LVITKTDLSPYVDVNAEELKKNALSINPKLQTFMTSAKTGEGIEEFVSFLVASSQSL"
     gene            2561..>3584
                     /locus_tag="VJC18_00665"
     CDS             2561..>3584
                     /locus_tag="VJC18_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HLD43918.1"
                     /translation="MKTTIIHIIGIVQGVGFRPFIYRLAQICQLNGIVYNHPKGVTVE
                     LQGDDHCINNFLQALPLEAPPLSRIDSVQTETAQDRQIYSDFTIVESRQEGKPSTLIS
                     PDVCVCDDCLREMFDSHDRRYLYPFINCTHCGPRYTIIKQLPYDRPGTTMKPFALCGD
                     CQIEYTNPLDRRFHAQPNACSVCGPRLKLWDAQQKEVTGDPVEKVIALLQQGKIVAIK
                     SLGGFHLAVDATNDAAVRRLRERKGRDEKPFAVMIGNLTSARQLANISPQAETLLQSR
                     ERPIVLIRGSSFSHSCDREFVSSCVRKLSASIAPHHHELGLILPPTPLHYLIFFHPHC
                     GGDFLAD"
BASE COUNT          907 a          944 c          802 g          931 t
ORIGIN      
        1 tcatcgagct tctttttgga gatatcgaga atcgtggtat tgccgcccat tccaatcgcc
       61 acatcggccg cgcaacgccc cacgacacca ccacccagaa tcaacacatt ggcaggttta
      121 acggtgcgag tcccacccat gagcacgcca cgaccacctt ggtcgaaccg aaggaaatgc
      181 gccccgttct gtgtggccag tttgccggca atttcactca tgggaatgag caaaggaagg
      241 gaaccgtctt cgagttgcag ggtttcaaaa ccaagtgcat gaatcttgct gttcagaatg
      301 gcgcgcatcg tatctggaat gggagccaga tgcagataac caaaaaagat ctggccttcg
      361 tgcatcatgt tgatttcttc gatggtcggt tccttgacct tgacaatcag gtctgatcgt
      421 tcaatcagct cttttgtctt ttcgacaatt tcggcacccg cctcgacata atcgcgatcc
      481 tcaaacccgg aaagcactcc agcattcttc tggatcaata cagtgtgtcc accagccttg
      541 agttgtttga cgttatcggg gatcagacaa acacgacctt cacgggtttt gacttctttg
      601 aggcatgcga taatcatata aaatctcttt ctttgtttgt ggtgccgccg tcctttgagg
      661 ccccccaaaa tgacagcgat cccttgaata gcgggactat aagtgctcct ttgacccgca
      721 agcaagtgat tttttatgat taagatcagc tgataaaaaa cgttgaatta tcaagcatag
      781 aatcatagta accgtcccat gacggagaaa accccaaaca ctgtttcggt cttcaacccc
      841 gccacctacc tgaccatcag ccgttttttt ctggtgcccg ttttcatcta tttttttctg
      901 gtcgccaaat ttgccatcgc cctggtcatt ttggtgattg ccagcatcac cgacatcacc
      961 gatggtctgt tagcccgtag attcaacatg gggaccaaga ttggctctgt cctcgatccc
     1021 ctggccgaca agtttttgat gctcatcagc tttctggtct tgagcacaca aaccattctt
     1081 ccctggcaat tggccaccat tgtcatcggc cgtgactttt acatcgtgtt tggggtcatg
     1141 tatctgtatt ttgtaaaaaa actcaaaatc catttccagc ccacaatgct gtcgaaaagg
     1201 acaaccttct tccagttcat gtgtctggcc ttttcttttc tcaaggtcta cctgacagcc
     1261 actccccagg ccctcacgca ggtctatacg atctattctt acaaatgcaa tttgaggatt
     1321 cttgccttat ccatgcacga tgtgatggtc ctcgtggcgg gtctgtttac tgcagccacg
     1381 ttcattcaat acacgattat ggggttgcag atcttgaagc gcaacgagaa gcgagaacca
     1441 cagatgattt gacgaaagca cgatggacga ccgttcgctg gcgcgaactt ggacgaagga
     1501 cgaaataaaa tttcttgatt gtttcattgt ttcattcttt gtataggccc gcacgtgcac
     1561 gaactatcga tcgccacaag cattgtcgaa atcgttgaaa acaatctcaa gacctaccct
     1621 acgggcaagg cgcgttcagt gacggtacaa ctaggcacac actgtggggt ggaaccaagc
     1681 gcgctggaat tttgttttcc gattgccatc gagggaacaa gggtccaagg ggcgcaattg
     1741 attttgcaaa ttctccccct gacactgaac tgctcatcct gcttggccca aaatgtgaca
     1801 gcctctgccc tgcaatgtcc tcactgtcac tcacctgaag tgacagtggt ctcaggcaga
     1861 gacatgattg tgacttcgat agatttggat gtaaaataat ttttttctat atgaccacac
     1921 taaacataaa atccaatgtc ctttccaaaa acgaccaagt ggcgcaagaa atcaaaaaga
     1981 tttttgccaa aaacaacatt acggcactga acctgattgg ctcacccggc agtggcaaga
     2041 ctgccttatt ggaagccacg ttcaaaaaac tccggggacg cttggactgt gccgtgattg
     2101 aaggcgatgt caaaacagat aacgataaaa agcggattga agccacggga gtccccgctg
     2161 tccaaatcga aaccgaaggc ggttgtcatc tcaacgccga acaaattttg aaggcactgg
     2221 aggcgtttcg tctggccgaa taccattgca tcatcatcga aaacgtgggc aatcttattt
     2281 gccccacctc ctatgatctg ggagaacgtt accgcgtgat agtcatcagt gttggcgaag
     2341 gtgtcgataa acccctcaaa tacccctcgg ccttcacatc cgcgaacgtc ctggtcatca
     2401 ctaaaaccga tttaagcccc tatgtcgatg tgaacgcaga ggaactcaaa aaaaatgccc
     2461 tgagcatcaa tcccaagctc cagaccttta tgacctcggc aaaaacggga gaaggcatcg
     2521 aggaatttgt tagttttttg gtggcttcat ctcaatcctt gtgaaaacaa cgatcatcca
     2581 catcatcggc atagttcagg gcgtaggatt cagacccttt atttatcgac tcgcgcagat
     2641 ttgccagctg aatgggattg tttataacca cccaaagggc gtgactgttg aactgcaggg
     2701 tgatgatcac tgtattaata attttttgca agccctgcct ttggaggcac cgccattatc
     2761 acgtatcgat tccgttcaaa ctgaaaccgc acaagacaga caaatttatt ctgacttcac
     2821 gatagtggag agtcgccagg aaggcaaacc ctccacattg atttcgcccg atgtctgtgt
     2881 ctgtgacgat tgtctcaggg aaatgtttga ttctcatgac aggcgttacc tgtatccgtt
     2941 tatcaattgc acccattgtg gtccccgtta cactatcatc aaacagttgc cctatgaccg
     3001 acccggcaca accatgaaac cctttgcgct ctgtggggac tgtcaaattg aatacaccaa
     3061 ccctttggac agacgctttc acgcgcaacc caatgcctgc tcagtgtgcg ggccgcgtct
     3121 gaagctgtgg gacgctcagc aaaaagaggt caccggtgat cccgttgaga aggtcatcgc
     3181 cctcctgcaa cagggaaaga ttgtggccat taaaagtctt ggtgggtttc atttggccgt
     3241 agacgccacc aatgacgcgg ccgtccgcag actgcgtgaa cgcaaaggac gagatgagaa
     3301 accctttgct gtcatgatag gtaacctgac cagcgctcgc caactagcga atatttcgcc
     3361 acaagcagaa actttgctac aatcccgcga acgccccatt gtattaattc gtggatcgtc
     3421 gttttctcat tcgtgcgatc gtgagttcgt gagttcgtgt gttcgtaagc tatctgccag
     3481 catcgcccca catcaccacg agctgggttt gatcctccct ccgacccctc tccactatct
     3541 gatttttttt cacccccatt gcggagggga ttttttggcc gacc
//
LOCUS       DATDUN010000050         1341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_500091, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000050 DATDUN010000000
VERSION     DATDUN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500091"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VJC18_00670"
     CDS             complement(<1..721)
                     /locus_tag="VJC18_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD43919.1"
                     /translation="MFDRFHRKINYLRISVTDLCNLRCVYCMPASGVPLKRHEDILSF
                     EEIEALTKVAVNLGIDKVRLTGGEPLVRRNILELVRRLGKLQGILDFAMTTNGVFLPQ
                     YALALKEAGLHRVNISLDCLDPKHFSELTRGGSVTDTLAGIEAALATGFRQVKINCVV
                     NESPDEPQARDVATYCRERGLEVRFIRKMDTAKGDFWRVIGGEGGHCDICNRIRVSSD
                     GHVFPCLFSNLKFSIREFGPEQ"
     gene            complement(699..>1341)
                     /locus_tag="VJC18_00675"
     CDS             complement(699..>1341)
                     /locus_tag="VJC18_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLD43920.1"
                     /translation="VEPDNTPGPAQIRNSNAWQLLAQVRSLGIEPCYSGILPDREDIT
                     SAAIDTAAKSSDVLLLSGGVSAGDYDLVPDILRKLGFLFAFDSVAMQPGRPTIFAHKE
                     QVYVCGLPGNPVSTFINFELLIRPFLYRLMGHDWQPLVVEARLAEAIRRKQTSRQSTV
                     PVRFVTPSEVAPVEYHGSAHIHALTRASGLVTIPVGQACLQKGDVVRVRSLSS"
BASE COUNT          327 a          357 c          370 g          287 t
ORIGIN      
        1 cttgttccgg gccaaactcg cgaatcgaaa atttgagatt gctgaacaaa cagggaaaga
       61 cgtgtccgtc gctggaaacg cgaatgcggt tgcagatatc gcaatgaccg ccctcgccgc
      121 caatcacccg ccaaaaatca cctttggcgg tgtccatttt tctgatgaaa cgaacttcca
      181 gaccccgctc ccgacaatag gtggctacgt ctctggcctg gggttcatcg ggggattcgt
      241 tcaccacaca attgattttt acctgacgaa atccggttgc cagggcagct tcaatgccgg
      301 cgagtgtgtc tgttacagag ccaccgcgcg tgagctcact gaagtgcttg ggatccaggc
      361 aatccagact gatgttgacg cggtgcaggc cagcttcttt gagggctaga gcgtattgtg
      421 gcaaaaaaac tccattggtg gtcatggcga aatccaaaat tccttgaagc ttgcccagcc
      481 gacgtaccag ttccagaatg ttgcgacgga ccagcggctc gccgcccgtg aggcgaacct
      541 tgtcaatacc cagattcaca gccactttgg tcaacgcctc aatttcttca aacgagagga
      601 tgtcctcatg tcgtttgagg ggcacaccgc tggcgggcat gcagtacaca caacgcaggt
      661 tgcacaaatc tgtcacagaa atgcgaaggt agtttatttt acgatgaaag cgatcgaaca
      721 cggacaacat ctcctttttg caaacaggct tgtcccacgg gaatggtgac caaaccactg
      781 gccctggtca gggcgtgaat gtgagccgat ccatgatact ccacgggggc cacctctgag
      841 ggtgtgacaa agcggacagg aaccgttgat tgacgcgagg tttgtttgcg tcgaatcgcc
      901 tctgccagac gggcttccac cacgagaggt tgccagtcgt gccccatcag ccggtacagg
      961 aatggtctga tgagcagttc aaaattgatg aaagtcgaga caggattacc gggcaacccg
     1021 caaacgtaga cctgttcctt atgagcaaag atcgtaggac ggccgggttg catggctacg
     1081 ctgtcgaagg caaagaggaa tcccaatttg cgcagaatgt cgggcaccag atcgtaatct
     1141 ccggcagaaa ccccgccaga gagcaacagc acatcggacg attttgcggc cgtatcaatg
     1201 gccgcagagg tgatgtcttc tcggtcaggc aatatgccgc tgtaacaagg ttcgattccc
     1261 agagagcgaa cctgcgccaa aagttgccaa gcgttgctgt tgcgaatctg agccggtccg
     1321 ggtgtgttgt caggttccac c
//
LOCUS       DATDUN010000051         1628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_168601, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000051 DATDUN010000000
VERSION     DATDUN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168601"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VJC18_00680"
     CDS             complement(<1..180)
                     /locus_tag="VJC18_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43921.1"
                     /translation="MTVVTQLVRLFPSGTTHSVPQQRAVTLSRYNPAVDPWNYDASRP
                     VGRHDARVSSHAETTC"
     gene            483..1064
                     /locus_tag="VJC18_00685"
     CDS             483..1064
                     /locus_tag="VJC18_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43922.1"
                     /translation="MDEYLLTLMRAPQSVFSHTDLALLWRKTAPSFLNKKIHRYLKAG
                     ALFPLRKGIYAKAQTFDPFELATKIFTPSYVGFETVLIRAGVVFQFYRQLFCASYLTR
                     EIIIHNQAYTYKKIKDAILTNAAGIETTSLYAIASVERAFLDTLYLYQDYHFDNLKPL
                     CWEKVFEILPLYHNKRLEKSVNRIHKDFLRENT"
     gene            1061..>1628
                     /locus_tag="VJC18_00690"
     CDS             1061..>1628
                     /locus_tag="VJC18_00690"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLD43923.1"
                     /translation="MKTAFDISKHRNVMLRILKDVYTHPLLGACLGFKGGTAAYLFYQ
                     LNRFSVDLDFDLLDPAKEDAVFSEMQNLLQQYGQLKDSAKKRFSLFFMLSYEGKVSNT
                     QNVKIEVNRRASTSQYQLKSYLGISMLVMAQGDMAAHKLMAMSERLHKANRDIYDAWF
                     FLHHGWPINRDIITQRAKQTYPAFIAKCV"
BASE COUNT          438 a          421 c          360 g          409 t
ORIGIN      
        1 acacgttgtt tcggcgtgcg aactcacgcg tgcgtcatga cggccaacag gacgagaagc
       61 atcataattc cagggatcga cagccggatt atagcgtgat agggttaccg cacgctgttg
      121 tgggacagag tgtgtcgtgc ctgatggaaa caacctaaca agttgagtga caactgtcat
      181 atagccacct ttcactacag attatcggtt gccctggata tttgttgttg tgttgcaaaa
      241 attatccgtg tcctcaagga cacagaactc gtggcaggtg gacacatcgg atatggaaca
      301 tgggggcaaa tataaaccaa acaggtttga ttttgcagga gccattcagt agcaactagt
      361 caaccagaga tttgacccca agggttttga ccccaagggt tctctccaag ggttcctctt
      421 gaccagcgtc tgtgattgat ataaactagt aacacccata gttgggtatt actagtttaa
      481 atatggacga atatctctta accctcatga gggcccccca gtctgtcttt tctcacactg
      541 acctagccct gctgtggaga aagacagccc cctcttttct caacaaaaag atccaccgct
      601 accttaaggc cggagccctg ttccccctgc gcaaagggat atatgcaaag gcgcaaacct
      661 ttgatccctt tgaattggcc acgaagattt tcacgccctc atacgttggt tttgaaaccg
      721 tgctgatacg cgccggggtg gtttttcaat tttaccggca attattttgt gcctcctacc
      781 tcacgcgcga gatcatcatt cataatcagg cctacacata caaaaaaatc aaggacgcca
      841 tcctgaccaa cgccgcgggc atcgaaacaa cctccttgta tgccatcgca tctgtggaac
      901 gcgccttttt ggatacgctc tatctctatc aggactacca cttcgataac ctcaaacccc
      961 tgtgttggga aaaagtgttc gaaattcttc ccctctatca caataagaga ttggagaagt
     1021 ctgttaacag gattcacaaa gattttttga gggaaaacac atgaagactg ccttcgacat
     1081 cagcaaacac agaaatgtca tgctcagaat tctcaaagat gtgtacacac atcccctgct
     1141 gggcgcgtgt ctgggattca agggaggaac agccgcctat ctgttctatc agttaaaccg
     1201 tttttctgtc gatttggatt ttgatctgct ggatcccgca aaagaagacg ctgtcttttc
     1261 ggaaatgcaa aacctcctgc aacaatacgg tcaactcaaa gattctgcaa aaaaacgttt
     1321 ttcactgttt ttcatgctgt cctatgaagg caaagtttcg aatacgcaaa acgtcaagat
     1381 agaggttaac cgtcgggcgt ccacatcaca gtaccaactc aaatcgtatc tgggcatttc
     1441 catgctggtc atggcccaag gcgacatggc cgcgcacaag ctcatggcca tgtccgaacg
     1501 cctgcacaag gccaatcgcg acatttatga tgcctggttt ttcctgcacc acgggtggcc
     1561 catcaacagg gacatcatca cccaaagggc caagcagacc taccccgcct tcattgcgaa
     1621 gtgtgtcg
//
LOCUS       DATDUN010000052         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_86848, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000052 DATDUN010000000
VERSION     DATDUN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86848"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VJC18_00695"
     CDS             <1..1004
                     /locus_tag="VJC18_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43924.1"
                     /translation="MTEADSDACHLLESAAQLLHDDETHSKTEKLLALSSVLLVENRR
                     VRTFDGYTDLLSRLNKQLRRLGIEKKKNEHVTALSGEFFVDENVGRLSAMLVKREGRF
                     DGVVVIPDLNWEVRFSLVPNGRAGQYIVDDSQPVSVTSLTTRRVVAAGISLERNEDNT
                     SEFNLVFRGETAISVFIMGTESRVERGVYPVFHEINTNADSKLILDALVTRSAKIIAP
                     DMRVIMGRLLSRNSLAAGTGFTAQYRLLCANGDVITVSQKVDQSGVRIKKIEASALWH
                     DLHLGTVSTMTVTQKGNALVLTGGGSNQEIIIPLTTAQTRMASHLLDLDKIEMRRRG"
     gene            complement(1212..>2336)
                     /locus_tag="VJC18_00700"
     CDS             complement(1212..>2336)
                     /locus_tag="VJC18_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43925.1"
                     /translation="LPLVAGQTGFEVEPALLWLFMKLSFPKGKPTEPLEDRYRKPNPP
                     EGTPDLKDLMKQPEYTVTYIINNRLTLRATWNHSTITAVSAEFDHTEIPCKIFSVRER
                     IFIALGGPLTGVIFSRYADSYVENATSTNHALWLQTLDEYLGTLSGDYNVHNLPYKPK
                     IMLWSEKIPGLTKENFHPDLRVDAEGQLLNPTHPILSADEMMSLRNAMLRQLGRARLH
                     AVDALLQEQELVPSDRRRFEIIDALYPVLVPNPESFKRFDNKGRPVPNKLSGVEFVMQ
                     YPQLRAGLAKVLWLVARGFVTIEQLDATRILSMAQTLLNRDPQSEIATRFVGFISDRC
                     DQLISASGYHFETTASAQVTSVPGLGGVFGTTVKHGDDVK"
BASE COUNT          595 a          512 c          615 g          614 t
ORIGIN      
        1 atatgacaga ggcagattcc gatgcgtgtc atctcttgga gagcgcggcc caactgttgc
       61 acgatgatga gacacactca aagaccgaaa agttgttggc gctgtcttct gtgttgctgg
      121 ttgaaaacag acgagtcagg acctttgacg gttatacaga tcttttgagt cgtctcaata
      181 aacagctccg tagattggga attgagaaga aaaaaaatga acatgttact gctttgtccg
      241 gtgaattctt tgtcgatgaa aatgtgggac gcctctccgc catgctcgtg aaaagagaag
      301 gtcgctttga cggggttgtt gtgatcccag acctgaactg ggaagtcagg ttctctcttg
      361 ttcccaatgg aagagcaggg cagtacattg ttgatgattc tcaacctgtg agcgtgactt
      421 ctctcacgac gaggagagtt gttgcggccg gcatcagtct ggaaagaaac gaagacaata
      481 catccgaatt caatcttgtg tttcggggcg aaacagccat ttcagttttc atcatgggga
      541 ctgaatcccg ggtggaaaga ggtgtttatc ctgttttcca tgagatcaat acaaatgccg
      601 attcaaaatt gatcttggac gcacttgtga caagaagcgc aaaaatcatt gcgccagaca
      661 tgagggtcat catgggacgc ctattgtctc gtaattcgct ggcggcagga actggtttta
      721 ctgcgcagta ccgcttgctt tgtgccaatg gtgatgtgat cacggtttcg caaaaagttg
      781 atcaatctgg tgttagaata aaaaaaattg aagccagtgc tttgtggcat gatttgcatc
      841 tggggacggt cagcacaatg actgtgacac aaaaaggcaa tgcccttgtg ctgaccggag
      901 gaggctcaaa tcaggaaatc atcatccctc tgaccaccgc gcagacacgc atggcatcac
      961 acctgttgga tctggataaa atcgagatga gacggagggg atgaggcagg gatggggaat
     1021 gaatatcgaa tgtcgaacaa ggaataacga atgtcgaagt ggggatttct tgtcatccca
     1081 gcgtcccgca gaatgggatc attcctgcgg ctgatgggct ggaatccgtt tgtgttgatt
     1141 ctggatccct gcctccgcag ggatgacata tttcgaaatt cgacattcct tgttcgatat
     1201 tgattatttc attattttac atcatcccca tgcttcacag tggttccaaa caccccaccc
     1261 aaacccggaa cgctcgtgac ttgcgccgat gctgtggttt caaagtggta tccagaagcg
     1321 ctgatgagtt gatcgcatct gtccgagatg aagccgacaa atctagtggc gatctctgat
     1381 tggggatccc gattgagaag tgtctgtgcc atactcagga tgcgggtggc atccaattgc
     1441 tctatggtca caaatccccg cgcgaccaac cacagaacct tggctagccc ggctctgagt
     1501 tgagggtatt gcatgacaaa ttcgacaccg ctcagtttat tgggcacggg acgtccctta
     1561 ttatcaaatc gcttaaatga ttccgggttg ggaaccaaca caggatacaa cgcgtcaatg
     1621 atctcaaagc gtctgcgatc cgaagggacc aactcttgtt cctgaagaag ggcgtcaaca
     1681 gcatgcaatc gcgctctgcc caactgtctg agcatggcat tgcgtagact catcatttca
     1741 tcggctgaca gaatgggatg agttggattg aggagttggc cttcggcatc gaccctgaga
     1801 tcggggtgaa aattttcttt ggtcaagcca ggaatttttt cactccaaag catgattttt
     1861 ggtttgtaag ggagattatg aacattataa tctccactca gagtgcccag atattcatcc
     1921 agagtctgca accagagtgc atggtttgtg ctggtggcat tttcgacata gctgtcggca
     1981 tagcggctga agatgactcc cgtgaggggt ccccccagtg cgatgaaaat gcgttctctc
     2041 acactgaata ttttacaagg aatttcagtg tggtcaaatt ctgcagaaac agcagtgatg
     2101 gtgctgtgat tccatgtggc cctgagtgtc agtctattat taatgatgta ggtcacagtg
     2161 tattctggtt gcttcatcaa gtctttcagg tcaggagttc cttctggagg gttgggtttg
     2221 cgataacgat cctcaagcgg ttcagtgggt ttgcctttgg gaaaggaaag cttcatgaag
     2281 agccacaaaa gcgccggttc aacctcaaaa cctgtttgtc ctgcaaccaa gggcag
//
LOCUS       DATDUN010000053         1909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_336136, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000053 DATDUN010000000
VERSION     DATDUN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336136"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VJC18_00705"
     CDS             complement(<1..198)
                     /locus_tag="VJC18_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43926.1"
                     /translation="MAFIGYKAPYLAPSIMNTTAERLADHLLFTQVHAPIVLNISPER
                     VQVLRRRAQVERSEIHKPPIRD"
     gene            461..1018
                     /locus_tag="VJC18_00710"
     CDS             461..1018
                     /locus_tag="VJC18_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HLD43927.1"
                     /translation="MPSLMQVHGFEPVAFSGASEFSLRGSSTCYVSSDTPNCGGGVVA
                     GQRSTQQAHVTRGVFRSVDMINLPGVSHVKLLALSQRFATLGIDEKDIIERFIRGSGK
                     GGQKINKTSNCVQLVHTPTGRCIRCQQSRDRNENRYLARCSLADQIENEMTSALLKQA
                     RQTHIKQVAERVRLSHLRRKVERFG"
     gene            1028..>1909
                     /locus_tag="VJC18_00715"
     CDS             1028..>1909
                     /locus_tag="VJC18_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HLD43928.1"
                     /translation="MPLTLNTNTQVPIVSWATRPMTGEEQATACQVANAPFVQPHVAL
                     MADVHHSSGVLVGSVIPTKGVVYPSAVGRDIGCGMLARKLTLRADLFKPKKLARARAE
                     IERLVAVGTNYHTNPEDDVLGWSGWDRFEQLHPEVRADHQRALTQMGTLGDGNHFIEI
                     CVEPDQGRESNIWVVLHSGSRGIGHQLGEIHFESAKIYTGQSGITVPNTFFSFLREGT
                     SEFAAYWSDMQWAMDYAQANRAIMMARIISYFAELFQQDENSIVAQTIECVHNYVARE
                     RHFGEDVFITRKGAIRAG"
BASE COUNT          522 a          398 c          492 g          497 t
ORIGIN      
        1 atctcgtatc ggtggtttat gaatttcact tcgctccacc tgggctcgcc gcctgagtac
       61 ttgtacacgt tcaggagaaa tattcaagac aataggcgcg tgtacttggg taaataacag
      121 atgatcagcc aatctttctg ccgtggtatt catgatgctg ggggcaagat atggtgcctt
      181 gtaacctata aaagccataa tcagaattcc ttttctacgt tgcactctcg tctaatccaa
      241 aaaaatgttg ctaaaaaaaa tgccctcatg agaatgttgt taatatatca atgataacaa
      301 aatgttggct tgtggttgcg tggtgtgagt gttatcgaat ttgcgcaaaa aaaataataa
      361 tttcacgcaa caagtgcgtt attttgacga taatgagcag ggtcggtgat ttttaaccaa
      421 tgttgtggat aatcgtttta tgtttattcc tagaatgggc atgccatctt taatgcaagt
      481 tcatggtttt gaacccgttg ccttttcggg ggcatcagaa ttttcactac gaggttccag
      541 cacttgttac gtgagttccg atacaccgaa ttgtggcggc ggtgtggttg ctggccagag
      601 atcgacacaa caagcacatg tgacaagagg cgtttttcga tccgttgaca tgatcaatct
      661 gccgggtgtt tcgcatgtca agttgcttgc attgagtcag cgatttgcga ccctcggtat
      721 cgacgaaaaa gatatcattg agcgttttat cagagggtct ggtaaggggg ggcaaaagat
      781 caacaagaca agcaattgtg tgcaacttgt ccacacacca acaggccgct gtattcggtg
      841 tcagcaatcg cgggacagaa acgagaaccg ttatctggcc agatgcagtt tggccgatca
      901 gatagaaaat gaaatgacca gcgcactgtt gaaacaggcc cgacagacac atattaaaca
      961 agttgccgag agggtgcgtc tatcgcacct cagacgtaag gttgaaagat ttggatgaat
     1021 tgtaaatatg cctctaacct taaacacaaa cactcaagtg cccattgtca gttgggccac
     1081 acgcccgatg acaggtgaag agcaggccac agcctgtcag gtggccaatg cgccatttgt
     1141 tcaacctcat gtagcgctca tggcagacgt gcatcactca tcgggtgttt tggttggctc
     1201 cgtcataccc accaaaggcg tcgtgtaccc ctcagccgtt ggcagggata ttgggtgtgg
     1261 catgctggca cgaaaactca cattgagggc ggatttgttt aaaccaaaaa aattggcaag
     1321 ggcaagggca gaaatcgaga ggcttgttgc cgtgggaacc aactaccaca cgaatcctga
     1381 agatgatgta cttggttgga gcggatggga tcggtttgag caattacacc cagaagtgag
     1441 agcagatcac caaagggcgt tgacacagat gggaaccttg ggcgacggca accattttat
     1501 tgaaatttgt gtggagcctg accagggtcg tgaatccaat atctgggttg tgttacacag
     1561 cggatcgcga gggattggtc atcagcttgg tgaaatccat tttgaatccg ccaaaattta
     1621 cacaggtcaa tcggggataa cagtgcccaa tacctttttt tcatttctga gagaagggac
     1681 gtctgaattt gcggcttatt ggagcgatat gcagtgggcc atggactatg cacaggcaaa
     1741 ccgtgcgatc atgatggcaa gaatcatcag ctattttgcc gaactgttcc agcaggatga
     1801 aaatagtatt gtggcacaaa cgatcgaatg cgtacacaac tatgttgcac gtgaaaggca
     1861 ttttggtgaa gatgttttta ttacccgcaa aggtgctatc cgcgccgga
//
LOCUS       DATDUN010000054         1615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_418032, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000054 DATDUN010000000
VERSION     DATDUN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418032"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VJC18_00720"
     CDS             <1..303
                     /locus_tag="VJC18_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43929.1"
                     /translation="TDKWAREFKKRSHEVVENVKNIDLIDQQIIDHMKDNIANAVDRV
                     SNSDVLDRAKEKVLTTKQQLLSALDLPTQQDIKDLAGKIVSLEKKVRTIGKRKNVA"
     gene            complement(639..1013)
                     /locus_tag="VJC18_00725"
     CDS             complement(639..1013)
                     /locus_tag="VJC18_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43930.1"
                     /translation="MKGFSVLTLVVVLLCQPVWAESKTNVKKGLLIGGLSGTAVGFGT
                     SFAIGQKYTCNANQKAQAEPCSLPTIITVSGTIGGAAIGTGIGALIGSLIPKKQNLSV
                     LPLVTHDDKLGTMGGLLLSKRY"
     gene            complement(1239..1315)
                     /locus_tag="VJC18_00730"
     tRNA            complement(1239..1315)
                     /locus_tag="VJC18_00730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1279..1281),aa:Arg,seq:tcg)
BASE COUNT          421 a          424 c          373 g          397 t
ORIGIN      
        1 accgacaaat gggcgcgtga gtttaaaaaa cgttcacacg aggtcgtgga gaatgtcaaa
       61 aatattgatc tcatcgatca acagatcatt gaccacatga aggacaacat tgccaacgcg
      121 gtcgatcgcg tgagcaattc cgatgtgctc gaccgggcca aggaaaaggt gttgaccacc
      181 aaacaacagc tgttgagcgc gcttgacctg ccgacccagc aggacatcaa agacctcgct
      241 ggcaagatcg tttcgctgga gaaaaaagtg cgaacgatcg gcaaacgcaa gaacgtggcg
      301 tgaggctagg gctaggggtc aagtctctgg ttgactagtt gttggcgtgt tatactgagc
      361 gacataaata tttgccgatt ccaagagctt taatgtcatt gcgaggagcg tagcgacgtg
      421 gcaatctccc tgttatcaat tgttgtaggc ggatcgccac gccggcgcat aacatcagaa
      481 ttgcctttgg gcaattccgc cgcaatgcac tgctcgcgat gacaagtcaa tgttttcagc
      541 acctacatga gacattgtgt cgagtttcct aaagcaacta gtcaaccaga gacttgactc
      601 ctagccttaa aaatagagat ttttgtttcc ataaaacctt aatatctctt ggacaacaat
      661 aagccgccca ttgtgcccag tttatcatca tgggtcacta gcgggagaac actcagattt
      721 tgtttcttgg gaataagact gccaatcaaa gcaccaatac ccgttccaat agctgcaccg
      781 ccaattgtac ctgaaactgt gataattgtg ggaagtgaac agggctcggc ttgagctttt
      841 tgatttgcgt tacatgtata tttttgccct attgcaaacg aggtcccaaa tccaacagct
      901 gttcctgata aacccccaat cagtagtcct tttttgacat ttgttttgga ttccgcccaa
      961 acaggctgac ataataaaac cacaacaagt gtcaaaacag agaatccttt cataagcact
     1021 cctgaaaaat ggttctaaac tcatgccggg ctagcctcgc aaacccagca caaatcaata
     1081 ttagaaacgt gcgcgcccgg agagattccc ctttacgcgc cttgcggcgc tccaaggact
     1141 tcctcgcgct ttaggcgcgc tcggtcggaa ccaagggagt tctcattccc atccggacac
     1201 gcaaaacaaa aaagcaccac aagggtgctc atttgttttg gcgcgcccgg agagattcga
     1261 actccctacc ctctggttcg aagccagatg ctctatccat atgagctacg ggcgcgttga
     1321 tcattcatct ctatagcaaa gacctgcttt gtttgtgaca aagagcctga cggctctggg
     1381 ctacgattga ttcctgaacg tcatcaatct ttagccccca cctcggctcg ttttcgcatg
     1441 ttcgctgggc cgctcacatg ctcctctggc cccgccagca ctcgcctcgg cttcggcgaa
     1501 ctaggccgtt cgcctcaccc atatgagcta cgggcgcgtt gatcattcat ctctatagca
     1561 aagacctgct ttgtttgtga caaagagcct gacggctctg ggctacgatt gattc
//
LOCUS       DATDUN010000055         4026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_336234, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000055 DATDUN010000000
VERSION     DATDUN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336234"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VJC18_00735"
     CDS             <1..625
                     /locus_tag="VJC18_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43931.1"
                     /translation="NAERRGYLHGINDETVARLLHKQALPFKHVTDFVTVAEYRDYHQ
                     REELWEKQLATFVVGVRILQEGFGWQGEERIPMMTSLDRVYELHHEGKKTASKSTQSN
                     VGEDSSNAEGAEFEKESLHDLIDLFQGKSLEDFSESDPADEPVSLPNQLDFEKYDGNI
                     VVSEEVAPEELENEARYLNSQALKDVEEETEAPESGEKIMSPICIKK"
     gene            1347..2420
                     /locus_tag="VJC18_00740"
     CDS             1347..2420
                     /locus_tag="VJC18_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD43932.1"
                     /translation="MVNVDDAYYPHTVALEVTLACNMRCLHCGSYATGQNRKKALSLA
                     EWKKVIDDLKQLKAQFITLSGGEPFMYKQWRELIKYIRRAPEAKASLISNGYSITEDD
                     IIFMKKQGVSHIGLSLDGDQTVHDYIRQTPGSFQKVLETARLCQKHDFSFSVVTSINQ
                     INYTIREELLGIILEHKIKNWQVQVVNSFGRAGKNRESMLISHEQYAQLVHDISRWQN
                     EQKGRVRIMPADSIGYCHPLTDDMLEGAEWQGCNAGKFVIGIESNGNVKGCLSLQHDD
                     FKAGNVRERSLIDIWNDDACFAYTRQYDHTRMCGACSTCSDGKRCQAGCMAIAHSVHG
                     TIYENGYCYKTIMEKMRHKRNAA"
     gene            2643..3833
                     /locus_tag="VJC18_00745"
     CDS             2643..3833
                     /locus_tag="VJC18_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43933.1"
                     /translation="MTLDRQILLNILALDKGFEEFEFIHSYLGPPIIRKLSAKIPVKM
                     AYENLHTLRELVRSDFDDEPPFYRATYVDDLIQSVLAQTEYFVLGVRSDSTKSLAERF
                     LGFETLPPYDLGEELRVLREGLDDYGYGSVGEYRKARQLLTFASPQQLSRYVHRLCDE
                     LSLRIKNRLAPLFHFDMAPLLNRSQLRVTATKKDDPPCFYRYLGDFHGMVGLSVKKNY
                     FRDYLENFITHEILPGHHFYYLVKQQYLNSGTGDALMAIDTYYSPENLINEGLAVNSD
                     LLFGEAWDARTSCTLAIDKYLHKIYHNAWHALNVSCGRPDPLCQDILESEFGLSRVEA
                     LTRLNYFTHEARWYTPSYSQGIHHVGHFINACGRALVPLLYGQHSVNTLKKLENYHEH
                     THAA"
     gene            3811..>4026
                     /locus_tag="VJC18_00750"
     CDS             3811..>4026
                     /locus_tag="VJC18_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43934.1"
                     /translation="MNTRTRPEGQLNFYSIIASTLLSNFGSFLNMMALNILILQITNS
                     AAWVGLVLGVRIISGMLVSPHLGVLSDR"
BASE COUNT         1081 a          885 c         1029 g         1031 t
ORIGIN      
        1 gaacgcggaa cgtcggggtt atttgcatgg gattaacgat gagaccgtgg ccagattgtt
       61 gcacaaacag gctctgccat tcaagcacgt gaccgatttt gtgaccgtgg ccgaatatag
      121 ggactatcat caaagagaag aactgtggga aaaacaattg gcaacctttg tcgtgggtgt
      181 gaggatcttg caagaaggtt ttggttggca gggagaggaa cgtattccca tgatgacgtc
      241 acttgataga gtgtatgagc ttcaccatga gggtaagaaa acagcatcaa agtctacaca
      301 aagtaatgta ggtgaagaca gcagtaatgc agaaggtgcg gaattcgaga aggaatcact
      361 tcatgacttg attgatctct ttcaaggcaa aagcctggaa gatttttctg aaagtgatcc
      421 cgctgacgaa ccagtatcac ttcccaatca gctcgatttt gaaaagtatg atgggaacat
      481 tgtcgtatcc gaagaggtgg ccccggaaga gttggaaaac gaagctcgct atttgaattc
      541 acaggcgttg aaagatgtcg aggaagagac ggaggctcct gagtctggcg agaaaatcat
      601 gtcgccgata tgtattaaaa aatgattaaa cagatggttg tgagacaggc gactgtcaca
      661 ttccacatat taataagcac acaggttcta acgaaagacc tttctctcta tttactttca
      721 taattattcc tatacaattt ttctcaagtt tgatgattct ttttcccgat acaatcggag
      781 gtgaaagaga tcaaaacaga gaagaggaat tatgggggtc agagggttta aaatttccac
      841 aagaaattaa atctcgatct cgtttctata accaataacg tccgttcact ccagagcgtc
      901 atcgctgtgg tgattcactc aggaggagac gtgacaagga gaaaacatga agaaagccaa
      961 gaaaaaaaga gccatgagac cgttttacgt cagcgagatg taggggggga gtttgtgctg
     1021 ggaggtcttg attcaatttt gaaagagccg caggacgcat aagaaaaatc ggagtacgca
     1081 atgtagggtc ctgcaggcat tcggacctga tgattcgtgt gttcagttga gatcattgta
     1141 ttgcagtagc gtctgtgcct ctctttcaaa attaaatcaa gacctcccag cacagttgag
     1201 taagaggggg cgttggcgca tgaatccaac aacataaaga gataccccag cacagttgga
     1261 tgggtgtttg aaattaatag tcttaacact gaatattcga cgttccttgt tccttgttcg
     1321 atattcgata ttcactggag gtagtcgtgg tcaatgttga tgatgcctat tatccgcata
     1381 cggtggcgtt ggaagtcacg ttggcctgca acatgcgttg cctgcattgc gggtcctacg
     1441 cgacaggaca gaatcgcaaa aaagcacttt cgctcgccga atggaaaaag gttattgacg
     1501 atctgaaaca actcaaggcc cagttcatca cgctgtctgg cggcgaacct tttatgtaca
     1561 aacagtggag agagctgatc aaatacatca ggagggcacc agaggcaaag gcctcattga
     1621 tcagcaatgg ttattccatt acggaagacg atattatttt catgaaaaag cagggtgtga
     1681 gccacattgg gctcagtctg gatggtgacc agactgtgca tgattacatc cgccagaccc
     1741 cgggttcctt tcaaaaggtg ctcgagacgg cgcgtctttg ccagaagcat gatttttctt
     1801 tttctgtcgt tacctcgatc aatcagatca attacaccat tcgtgaagaa ctgctcggca
     1861 tcatcttgga gcacaaaata aaaaactggc aggtgcaggt ggtcaattcc tttggtcgcg
     1921 ctggcaagaa tcgcgaaagc atgctgatct cacatgaaca gtacgctcaa ttggtgcacg
     1981 acatttcgcg ttggcagaat gagcagaagg gccgcgtgag gatcatgcca gccgattcca
     2041 taggctactg tcatcctctg accgatgaca tgttggaggg ggccgaatgg caagggtgta
     2101 atgccggaaa gtttgtcatt ggtattgagt caaatggaaa cgtcaaaggc tgtttgtcat
     2161 tacagcacga tgatttcaag gcaggcaatg tccgtgaacg cagtctgatc gacatttgga
     2221 atgatgacgc ctgttttgcc tacacgcgtc agtatgacca caccaggatg tgtggggcct
     2281 gctcaacgtg ctcggacggc aaacgctgtc aggccgggtg catggccatt gcccattccg
     2341 tgcatggtac gatttacgag aatgggtatt gttacaaaac catcatggag aaaatgcggc
     2401 acaaaagaaa cgcggcatga gtgatgacgt gttcactcgt tcgtacgtaa aaatgtaaat
     2461 ggttttgtca ttcctgcgaa ggcaggaatc cattattgat agattgaaac tctggatccc
     2521 tgctttggtt cgacaagctc accatcctga gtgccagtcg aaggatcgca gggatgacaa
     2581 acgattcatg ccctcagggg caccagcgat taacgattta caatccacga tccacgtgat
     2641 ttatgaccct cgaccgacaa atactgctca acattctcgc cctcgacaag gggtttgagg
     2701 agtttgagtt cattcattcc tatttggggc ctcccatcat ccgtaaactc tcagccaaaa
     2761 ttcccgtcaa aatggcgtat gaaaatttgc ataccctcag ggaactggtt cgttcggatt
     2821 ttgatgacga acccccattt tatcgtgcga cctatgttga tgatctgatt cagtcggtgt
     2881 tggcgcagac cgaatatttt gttttgggag tcaggagtga cagcaccaaa tcactggcgg
     2941 agagattttt gggatttgaa accctgccac catacgattt gggagaagag ttgcgagtgc
     3001 tcagggaagg tctggacgat tatgggtatg gttctgtggg agagtacaga aaggcacgtc
     3061 agctcttgac ctttgccagc ccgcaacaac tttcacggta cgtgcacagg ctttgcgacg
     3121 aattgtccct acggatcaaa aaccgactgg cccccctgtt tcatttcgac atggcccccc
     3181 tcctgaacag atcccagttg cgtgtgacgg ccaccaaaaa ggatgatccc ccctgttttt
     3241 acaggtatct gggagatttc catgggatgg tggggctgag cgtgaagaaa aactacttca
     3301 gggattatct cgaaaatttc atcacacacg aaatcttgcc ggggcatcat ttttactatc
     3361 tggtcaagca acaatacctg aactcaggaa ctggtgacgc cttgatggct atcgatactt
     3421 attattctcc ggagaatctc atcaatgaag gattggcggt caattccgat ctcctgtttg
     3481 gagaagcctg ggatgcccgc acctcctgca cattggcgat cgacaagtat ctgcacaaga
     3541 tttaccacaa tgcgtggcac gcactcaatg tgagctgtgg tcgaccagat cccctgtgtc
     3601 aggacattct tgaaagcgag ttcggtttga gtcgtgtcga ggcgctgacc cggctcaatt
     3661 attttacaca tgaggccaga tggtacacgc ccagttattc gcagggcatt catcatgtgg
     3721 ggcattttat caacgcctgt ggccgcgctc ttgtcccgct tttgtatggc caacattctg
     3781 tcaacacact caagaaactg gaaaattatc atgaacacac gcacgcggcc tgaggggcag
     3841 ctgaattttt attccatcat cgcttctacg ctcctctcca actttggcag ttttttgaac
     3901 atgatggcgc tcaacatatt gattctgcag ataaccaatt ctgcggcttg ggtgggattg
     3961 gtgctggggg ttcggatcat ttcgggcatg ttggtatcac cccatctggg agtcctgtca
     4021 gaccgg
//
LOCUS       DATDUN010000056         7984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_127080, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000056 DATDUN010000000
VERSION     DATDUN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127080"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /gene="lysS"
                     /locus_tag="VJC18_00755"
     CDS             complement(<1..1368)
                     /gene="lysS"
                     /locus_tag="VJC18_00755"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HLD43935.1"
                     /translation="MTQENIYRQQRLEKAQSLREAGINPYTNSLKPTHLAADLRDKFE
                     PKTTEELEGLHESCSIAGRVLAIRSFGKAGFMKLRDRTGEFQIFTQKTKMGEQGDLVL
                     KFAEVGDFVWARGVLFRTKTNELTLRVEDLRLVTKSVEQLPEKWHGLTDVETRYRQRY
                     VDMIANADVKEVFKKRSQIVSFIRHYFEQKDFLEVETPMMQVIPGGAKARPFVTHHNT
                     LDMDLYLRIAPELYLKRLVVGGLERVFEINRNFRNEGIDTQHNPEFTMLEFYQAYATY
                     EDLIVLTEDLFNQLATTVCGSENITYQEKEISFARPFARYTMAEAIEKHLGYTNAQVR
                     DRDFLIAECDKRHFEVKDKQNADLGIVQVILFEELVENKLISPTFITHYPTSVSPLSR
                     RNDADPSVTDRFEIFINGKEIANAFSELNDPVDQKERFVKQVAQRATGDDEAMFLDED
                     YVTALE"
     gene            complement(1535..2587)
                     /locus_tag="VJC18_00760"
     CDS             complement(1535..2587)
                     /locus_tag="VJC18_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLD43936.1"
                     /translation="MKSSELIREKRFIVILLLGLLLGFAYMIRPFLMSMLVAGIMAVV
                     FYPLFNKLSVFLGNRRRMSALTMTIFILFMIIIPAGYVITVLIDQIYQYVGTLNLNEL
                     FSQLFTTNIYAEKIAPFLTDLEMRYQIKIDLFGMLTNLGKQVAGFIGSYSPSVLMGTA
                     GFLFSFLITLVGIYFLFVDGPALVRLIFDLSPLQTRYQTRLARRLRDTLDACVYGYLV
                     SGILLAFISSFMYGIVGIQAYVILGALTFFMSMVPIVGTLGVWVPVCVWLFSNGQVWE
                     GVVVIVGGCVVAFIDYFAKPLIIKGKTQIHPLLIFFSLLGGIGMFGLLGVVFGPVITA
                     LMIAMIKIYQEEFAHS"
     gene            complement(2718..2846)
                     /locus_tag="VJC18_00765"
     CDS             complement(2718..2846)
                     /locus_tag="VJC18_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HLD43937.1"
                     /translation="MVKILESFGVNELLVVCLTALLIFGAKRITKWGSNFKKWVRS"
     gene            3060..3130
                     /locus_tag="VJC18_00770"
     tRNA            3060..3130
                     /locus_tag="VJC18_00770"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3092..3094,aa:Gln,seq:ctg)
     gene            3132..4259
                     /locus_tag="VJC18_00775"
     CDS             3132..4259
                     /locus_tag="VJC18_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLD43938.1"
                     /translation="MNPSFKRPFTALGFFVILAVSLFIAEFIVMLSLSGIHHELLPVA
                     EALIDALLLIFVLSPVLYFFAIKPLTDQIRLRYKSEMALMQQNKTFQKVNDILEQSLL
                     TENHTELARIFLQITNEQTSSDFGFIGLITEKQTLNIIATSDPALAKINPERFTELPV
                     SDEWMSPLLDNKTLLVNHQKDCRVVLGLPSWHPEITSFLSTTLRLKDGLVGIICLAKK
                     KFPFSGNDTSFIEALSPFFATAIHRKLLETKIKENEERLRTVTMTALDAIIIMDENGC
                     ITGWNPAATRMFGYPLDEILGKNLHTVLSPSRYWADYEKNIPHFRKTGQGPIVRKTRE
                     IVALRKDGTEFPIELSVSAIPFKDKWGAVAIVRDISNRAGS"
     gene            complement(4334..4837)
                     /locus_tag="VJC18_00780"
     CDS             complement(4334..4837)
                     /locus_tag="VJC18_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43939.1"
                     /translation="MKKINELISNMTKRDIGFRIDEVMTGHHEFEKNCGPKGRLFMEF
                     RGQWGPDDLRNWINPASEVFFKQKLSGTVTVEGLCRDVPMTGELALNYFKDASIKYSF
                     EFKARQKGYCYVGQKVNIKPWNLLFSHTTCFGRITEKKTGKLVSTSVVYFKLLSLPKF
                     LASLKLV"
     gene            complement(4975..5793)
                     /locus_tag="VJC18_00785"
     CDS             complement(4975..5793)
                     /locus_tag="VJC18_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLD43940.1"
                     /translation="MKNVKDKIVLITGAARGIGFCTAELFAKAGSVVLLTDIDARALS
                     QAKIKLKRQHAKACIHSYVVDIAKPKSVANLRKNIIKKFGRLDILINNAGIGYTGELV
                     ETKMETWKKLIDVNFWGCLNMTYVFLPDMIKKLSGHIVNVSSGQAFFRLPTWGAYAAV
                     KLAMACFSEVLHYEVKKFHIDVSTVYPFMVDTGFYDHVKPETFAAKLSMRLVPYYSMK
                     PESVAEIIFEAVRKQHNVERVSFLNDIAYYGSVIPYFSDTISRISNYVLTKRRA"
     gene            complement(5790..6410)
                     /locus_tag="VJC18_00790"
     CDS             complement(5790..6410)
                     /locus_tag="VJC18_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLD43941.1"
                     /translation="MGLLRTQKNQERKDQTRKALMGAAVSTFSKKGYHNTLISDIVTE
                     ARVGQGTFYRHFTDKREIFLNLKAEFVSNLISQFSEMSTHLPQNFAQYRQASIGAVTR
                     LAHQLNANKKFVLCLIKEAPTIDKELEGDVTGIFERFAELAKMYLDHAIRQGFARPCH
                     SGIVSHAIVGMGVQILMQWLEGRFNDVSVDRLVEELVDFGFLGVKK"
     gene            6704..7297
                     /locus_tag="VJC18_00795"
     CDS             6704..7297
                     /locus_tag="VJC18_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLD43942.1"
                     /translation="MPLSPSITGCCHSGDNSRFIRAYVMLKINQVADRIQIFHKLTDL
                     QNTVSCHAVRDLYDLVVELTAPSSTELDILINTKIMSLKGIDHLDRYVALNDPTPCDQ
                     TFHPHCQDSITEGATNVSAFILIEGHKEKLTQLKSSLCQIEGTLSCLCVAEKVDVILR
                     VTRKNFRDIHNTAQQQIRQLDGVTRVRVLPIIDLFEI"
     gene            7311..7862
                     /gene="nuoE"
                     /locus_tag="VJC18_00800"
     CDS             7311..7862
                     /gene="nuoE"
                     /locus_tag="VJC18_00800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HLD43943.1"
                     /translation="MTDSQTQVLSSAQPTSPKINIDLWQSRAQPGELIPLLQSAQESC
                     GYVTPQAIEYISQVTGNTKSEIYGVITFYKQFRLKPIGKFLIRVCDGTACHVNDAKTL
                     ISIVEDELKLEGDDTTADGLFTLQPVACLGCCSLAPALMVNDQTYGRLTPQKVRKILK
                     QYKNSSKVKGEKRISNIEQGMSK"
BASE COUNT         2128 a         2122 c         1735 g         1999 t
ORIGIN      
        1 ttccaaagcc gtcacataat cttcgtccag aaacatggcc tcatcatcgc ccgtggccct
       61 ttgcgccacc tgtttgacaa agcgctcctt ttgatccacc ggatcattga gctccgaaaa
      121 ggcattggca atctctttgc cgttgataaa aatttcgaac cgatccgtca ccgatgggtc
      181 cgcgtcgttg cgacggctta gtggcgaaac gctggtcggg taatgtgtga tgaacgtggg
      241 tgagatgagt ttgttctcca ccaattcctc aaacaagatc acctgcacaa tgcccaaatc
      301 cgcgttctgc ttgtctttga cctcaaagtg acgtttgtca cattcggcga tcagaaaatc
      361 gcgatcacgt acctgggcat tggtatagcc caagtgtttt tcaatagcct ctgccatcgt
      421 gtagcgagcg aacggacggg cgaatgaaat ctctttttcc tgataagtga tgttctctga
      481 tccgcacacc gtggtggcca actgattgaa caaatcctct gtcagcacaa tgaggtcctc
      541 ataagtcgca taggcctgat aaaactcaag catcgtgaac tcaggattgt gctgggtatc
      601 gatcccctca ttacggaaat tgcggttgat ctcgaacaca cgctccaaac cacccacaac
      661 gagtcttttg agatacaact ctggcgcgat gcgcaggtac aagtccatgt cgagcgtgtt
      721 gtgatgcgtc acgaacggcc tggccttggc cccgccagga ataacctgca tcatcggagt
      781 ttcgacctcc aaaaaatctt tttgttcaaa atagtgccgt atgaacgaaa caatctgcga
      841 gcgtttttta aatacctctt tgacgtcagc attggcgatc atgtcgacat aacgttgacg
      901 atagcgtgtt tcgacatcgg tgagaccgtg ccatttctcg ggcagttgtt ccacagattt
      961 tgtgaccaga cgcaaatctt ccaccctcag cgtgagctcg tttgttttgg tcctgaacaa
     1021 aacgccgcgc gcccaaacaa aatcacccac ctcagcaaat ttaagcacca gatcgccctg
     1081 ctcacccatt ttggtttttt gcgtaaaaat ctgaaactcg ccagtacggt ccctgagttt
     1141 cataaaaccg gccttgccaa aggagcgaat ggccaacacg cgacccgcga tactgcagga
     1201 ttcatggaga ccttcaagct cttcggttgt tttgggttcg aacttgtcgc gcaagtcagc
     1261 tgccaaatga gtaggcttca gtgaattggt gtaggggtta attcctgcct cacgcagact
     1321 ctgcgccttt tccaatcgtt gctgtctgta aatgttctct tgtgtcatag ttccttcctg
     1381 ataatgtgac catgtttttt ttgcgggtcc tgctccgggt cgctattttt ttgactttcc
     1441 gctcgcaagc tcgcttcgtc acgcagaacg tgacgcagtc aaaaataaca gctccccgta
     1501 tcacccacaa aaaaacaatt cccgaatatt atatttatga atgtgcaaac tcttcctgat
     1561 aaattttgat catggcgatc atcaaggccg tgatcacggg accaaacacc acccccagaa
     1621 gtccaaacat accgatccct ccaagaagcg aaaagaaaat cagcagggga tgaatctgag
     1681 tcttgccctt gatgatgagc ggtttggcaa aataatcaat gaaggccacg acgcaacctc
     1741 ccacgatcac caccacgcct tcccaaacct gcccattcga aaacagccac acacaaaccg
     1801 gcacccacac gcccagcgtg cccacaatag gtaccatgga catgaaaaac gtgagcgccc
     1861 ccagaatcac ataggcctga atccccacaa tcccatacat gaaactcgag atgaaagcga
     1921 gcaggatgcc cgagaccaga tacccgtaaa cacaggcatc gagtgtatcg cgcaggcgtc
     1981 tggccaaccg ggtttgatag cgggtctgca ggggcgacaa gtcaaatata agtctcacca
     2041 aggcgggccc atctacgaat aaaaagtaaa tgccaaccaa tgtgatcaga aaactgaaca
     2101 gaaaaccggc tgtgcccatc agcaccgatg gcgaatagct cccgataaaa ccggccacct
     2161 gtttacccaa attggtcaac atgccaaaaa ggtcgatttt aatctgataa cgcatttcca
     2221 gatctgtcag aaacggcgca attttttcgg catagatgtt ggtcgtaaac aactgcgaaa
     2281 aaagttcgtt gagattcagg gtacccacgt actgataaat ctgatcgatc agaaccgtaa
     2341 tcacataacc tgccgggatg atgatcatga acaggatgaa tatggtcatg gtcagggcag
     2401 acattcgacg tctgtttccc aagaacacac tcaatttatt gaaaagaggg taaaacacca
     2461 cggccatgat cccggccacc agcatggaca tcagaaaggg ccgaatcatg taggcaaagc
     2521 ccaacaggag ccccaacaag aggatgacaa taaaacgttt ttctctgatc aactcacttg
     2581 atttcatata tcatggtaac tcaaaactgt aaaagcgccc gccgacagcc tacagctaac
     2641 agccaaccgc caaagctaat ataatggccg tgggcagtcg accgtaagcc gtcggcagaa
     2701 tattttgctt tgagctttca gcttcggacc catttcttaa aattcgaccc ccacttggtg
     2761 attcttttgg ccccaaaaat cagcaaggcg gtgaggcaaa ccacaagcaa ttcgttcaca
     2821 ccaaaagatt ccagtatctt gaccataaaa acctccacag gaaggccctc acttacaaat
     2881 taacgggtaa aagtaaatga ttgattggga gaacgatatt tcttgaatat tcatgaataa
     2941 tgattaatag ggttcgcggt tctttctccg ggatcgtctc ctagaggttg ttaatgagca
     3001 aagcgaatgt tacaacctct tggaccccgc tttgcggggt aagcgacatc cttttttatt
     3061 ccgggatcgt ctaatggcag gacagcggac tctgactccg ctagtctagg ttcgagtcct
     3121 agtcccggaa catgaaccca tccttcaagc ggccgttcac ggcccttggt ttcttcgtta
     3181 ttctcgctgt ttctctgttc atcgctgaat tcatcgttat gctttcgttg agtggtatcc
     3241 accacgaatt gttgcccgtg gcagaggccc tcattgacgc ccttttgctc attttcgttc
     3301 tgtcaccggt tctgtatttt ttcgccatca aaccccttac ggatcaaatc agattgcgtt
     3361 acaagtccga gatggccctc atgcaacaga ataaaacatt tcagaaggtc aacgacatcc
     3421 tcgaacaaag tttgctaaca gaaaatcaca ctgaattagc gagaattttt ttacaaatca
     3481 cgaacgaaca aacctcaagt gactttggat tcattggatt gatcaccgaa aaacaaaccc
     3541 tcaacatcat cgccaccagc gatccggctc tcgccaagat caacccggag cgttttaccg
     3601 aactgcccgt ctcggacgaa tggatgtcac cactgctcga caacaagaca ttactggtca
     3661 atcatcaaaa agactgccgg gtcgttttgg gtcttcccag ttggcatccg gaaatcacat
     3721 cttttttatc cacgaccctc aggctcaagg acggcttggt tggcatcatc tgtctggcca
     3781 aaaagaaatt tcccttcagc ggaaacgaca cgtcctttat agaagccctc tctccctttt
     3841 tcgctacggc aattcaccga aagctcctcg aaacgaaaat caaggaaaac gaggaacgcc
     3901 tgaggaccgt caccatgaca gctctggacg ccatcatcat catggatgag aacggttgca
     3961 tcaccggctg gaacccagca gccaccagaa tgtttggtta tcccctcgat gagattcttg
     4021 gcaaaaacct gcacacggtc ctaagcccta gccgttactg ggcagattac gaaaaaaaca
     4081 tcccccattt ccgcaaaacc ggtcagggac ccatcgttag aaaaacccgc gagattgttg
     4141 ctctcagaaa agatggcacg gaatttccta tcgaactctc cgtatcggcg attcccttca
     4201 aagacaagtg gggagcggtc gcgattgtac gtgatatttc gaatagagcc ggttcttaga
     4261 ggcggcctat aatctgtatt gtggattgcc acgacccgtc agtcgtcggg cctcgcaatg
     4321 acaaatttct gaattacacc aacttcagac tcgccaggaa tttgggcaga ctcagcaact
     4381 tgaagtagac gaccgatgtc gaaacaagtt tgcccgtctt tttttctgtg atcctgccaa
     4441 aacatgtggt gtgcgagaaa agcaggttcc agggcttgat gttcaccttc tgccccacat
     4501 aacaataacc cttttgtcgc gccttgaact caaaactgta tttgatggat gcgtccttga
     4561 agtaattgag ggccagctca cccgtcatgg ggacgtctct gcacaatccc tcaacagtca
     4621 ctgtgccaga cagtttttgc ttaaaaaaga cctcgctcgc aggattgatc cagttcctga
     4681 gatcgtccgg cccccactgg ccacgaaact ccatgaacaa ccgccccttg ggcccgcaat
     4741 ttttttcgaa ctcgtgatgc cctgtcatga cctcatcgat tctaaaaccg atatccctct
     4801 ttgtcatatt actgatcagt tcatttattt ttttcataga aaatctcccc ccatatcggg
     4861 gttgtcaatt ttgccttttg cgggccggtc tcgaccggcc cctacgtatt atgactgcct
     4921 ttttttgcgt aggggcgggg cttgacccgc ccaaaagcgg aataatggac tttctcaagc
     4981 cctcctcttc gtcagcacat agttcgaaat cctgctgatc gtgtcggaaa aatagggaat
     5041 cacgctccca taatacgcga tgtcgttcaa aaagctcaca cgctccacat tgtgttgttt
     5101 tcgcaccgcc tcgaatatga tctcagccac cgactcgggc ttcatggaat aatagggcac
     5161 tagcctcatc gacaacttgg ccgcaaaggt ttcgggtttg acgtgatcat aaaaacctgt
     5221 atccaccata aaggggtaca ccgtactcac gtcgatatga aactttttga cctcgtaatg
     5281 caagacctcg gaaaagcacg ccatcgccag cttgaccgcc gcgtacgcgc cccacgtagg
     5341 gagtctgaaa aatgcctgcc ccgaagacac gttcacaata tgaccggata attttttgat
     5401 catgtcaggc aaaaaaacgt aagtcatgtt aagacagccc caaaaattga catcaatgag
     5461 ttttttccag gtttccattt tggtctctac gagttctccc gtataaccaa tgcccgcgtt
     5521 gttgatgaga atatcaagcc tgccaaattt tttgatgata ttttttctca gattcgcaac
     5581 cgatttcggc ttggcaatat cgaccacata gctgtgaatg cacgccttgg cgtgttgcct
     5641 cttgagtttg atttttgcct gactgagcgc tctcgcgtca atatccgtga ggagcacgac
     5701 actgcctgcc ttggcaaaca gttctgctgt gcaaaaccct atcccacgag ccgcacctgt
     5761 aatgaggaca attttgtctt tgacgttttt cattttttga ctcccaggaa accaaaatcc
     5821 acaagctctt cgaccagccg atccacggac acatcgttga aacgcccctc caaccactgc
     5881 atgaggatct gcacacccat gccaacaatg gcatgcgaga caatccccga atggcatggt
     5941 ctggcaaacc cttgcctgat ggcatgatcc agatacattt tggccagttc cgcaaacctc
     6001 tcgaaaattc ccgtcacgtc accttccaat tccttgtcaa tcgtgggggc ctctttgatc
     6061 agacacagaa caaatttttt attggcatta agttgatgcg ccaatcgtgt cacggcaccg
     6121 atggatgcct ggcgatattg cgcaaaattc tggggcaaat gggtcgacat ctcgctgaat
     6181 tgtgagatga gattggaaac aaattcggcc ttcaggttta agaaaatctc tctcttatca
     6241 gtaaaatgac ggtaaaaggt tccctgcccc accctggcct cggtcacaat gtctgaaatc
     6301 agcgtgttgt gatagccttt tttcgaaaag gtcgacacgg cggcacccat cagagccttt
     6361 ctcgtttgat cttttcgctc ctgattcttt tgagtccgta atagtcccat accgatttta
     6421 gcctatctta tgcggacgga ctagtcaatc cgttttgtat gtcatttgat aagtaatcgg
     6481 tcactcattg gagcattttg atttttgggg tgatacgggg agctattatt ttgatgcggc
     6541 ctgcggagca agccccgctt gcgaagagcg tcaaaaaata gcgacccgga gcaggacccg
     6601 aaaaatcaaa acgcttcagt gacgcattac taagattaat gaaattcgct ttacattttc
     6661 tgctttttga aaatatcgtg ccgcgaccaa taagggaggt ctcatgccgc taagtccatc
     6721 gattacgggt tgttgccatt caggcgacaa ttcccgtttc atccgtgcgt atgtcatgct
     6781 caaaattaat caggttgcgg accgcatcca gatttttcac aagctcacag atttgcaaaa
     6841 cacagtctca tgccacgcgg tgcgcgatct ctacgatctc gttgtggaac tcactgcccc
     6901 ctcctccacg gaattggaca tccttatcaa cacaaagatc atgtccctga aggggattga
     6961 tcatctggat aggtatgtgg ccctcaatga ccccacaccc tgtgaccaga cctttcatcc
     7021 ccattgtcaa gacagcataa cagaaggcgc aaccaacgta tcggctttca ttctgattga
     7081 gggccataaa gaaaaactga cacaattgaa atcgtcgcta tgtcaaatcg aaggcactct
     7141 ttcctgtttg tgcgttgcag aaaaagtcga cgtcattttg cgcgtcaccc gcaagaattt
     7201 tagagacatt cacaataccg cccaacagca gattcgacaa ctcgacggcg ttacccgagt
     7261 ccgggtactg cccatcattg acctttttga aatataacaa ggacccagat atgaccgatt
     7321 cacaaactca ggtgttgagt tctgctcagc ccacaagccc caaaatcaac attgatctct
     7381 ggcaatcccg cgcgcagccg ggggaattga tcccgctcct gcaaagcgcg caagagtcct
     7441 gtggttatgt cacccctcag gccattgagt acatcagcca ggtgacaggc aacaccaaga
     7501 gcgaaattta cggtgtgatt actttttaca aacagttccg cctcaaaccc attggcaaat
     7561 ttttgatccg ggtttgcgat ggcacagcat gtcatgtcaa cgacgccaag accctcatca
     7621 gcatcgttga ggacgaactc aaactcgaag gcgacgacac gacagcagat gggctgttca
     7681 cgctgcaacc agtggcctgc ctgggttgtt gcagtctggc cccggccctc atggtcaacg
     7741 accagacgta cggtaggctc acaccgcaga aggtgagaaa gattttgaag caatataaaa
     7801 acagctcgaa ggtgaaaggt gaaaaacgaa tatcgaatat cgaacaagga atgtcgaagt
     7861 gaaagtcatt ccagcgcaag ctggaatcca tgtttacatt aattctggat ccctgccttc
     7921 gcagggatga caaatttcaa cattcgacat tccttgttcg atattcagta ttcttgttcc
     7981 tttg
//
LOCUS       DATDUN010000057         1495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_293476, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000057 DATDUN010000000
VERSION     DATDUN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293476"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VJC18_00805"
     CDS             complement(<1..658)
                     /locus_tag="VJC18_00805"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLD43944.1"
                     /translation="MSEETNKDPEFTTAIIAPDETRDLISLRKYQLIVISKDGNRRKF
                     ELGKKKIIRLGKKADNDIVVNDKTVSRYHSEIHFTDDNSYLVKDINSTNGTTINGMKV
                     KEAYLSQGDLIEIGETKIEFQTYDESVQIEPSTTNIFGDMVGKSRKMRQIFGILERIS
                     QSQATVIIEGETGTGKELVARAIHQNSPRKDKPFIVFDCSSVAPNLIESELFGHTKGS
                     F"
     gene            complement(714..>1495)
                     /locus_tag="VJC18_00810"
     CDS             complement(714..>1495)
                     /locus_tag="VJC18_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HLD43945.1"
                     /translation="AYTLTVVSNPGGAEILVNGIPSGFQTPHVITSLEVGKKYLITVS
                     KDGYLTNEQEIVNNDQGDKSIDITLSALPKASLVISSQPEGATIYLGGEKTAFETPHT
                     VDALDIPQKILITLKKQGYEDAIEAVVLTAAKEYPVGLTLNKIPVIPTEKITTPHNTS
                     QTETTSSSTTTSKTKDSFSAFNTTNSNLAQLGKLRIDSRPAGASVSINGTPKGITPMV
                     VGDLPKNTALSVTVLKKGYSAWSRTVRLSQDRLEINAILAP"
BASE COUNT          324 a          328 c          375 g          468 t
ORIGIN      
        1 tgaacgatcc cttggtgtgg ccaaagagtt ccgattcgat caagttgggc gctacagaag
       61 aacaatcaaa cacgatgaag ggcttgtctt tgcgaggtga attctggtga atagccctgg
      121 ccacaagttc tttgccagtc cctgtttcac cttcgataat cactgttgcc tgagactggc
      181 tgatccgttc taatattccg aaaatctgtc gcattttgcg actttttccg accatatcgc
      241 caaaaatatt ggtcgtgctg ggttcgatct ggaccgattc atcataggtc tgaaattcaa
      301 ttttggtctc tccaatttcg atcaggtcgc cctgcgacag gtaagcttcc ttgaccttca
      361 tgccattgat ggtggtccca tttgtagagt tgatgtcctt gaccagataa ctgttatcat
      421 cagtaaagtg tatttcggaa tgataacgcg acaccgtttt atcgttgacc acaatgtcat
      481 tgtcggcctt tttgcccaag cgaatgattt tctttttgcc gagttcgaac ttacgccgat
      541 tgccgtcttt cgaaatgacg atgagctgat atttacgaag tgagatgagg tccctggttt
      601 cgtccggggc aatgatggct gttgtgaact cgggatcttt gttagtttct tcagacatat
      661 gtcgttctcc ttgataatat ttcgccgcgt gcatcactta gcgacgtcac ttgttaggga
      721 gccaaaatgg cattgatctc gagcctgtct tgcgacagac gaacagtgcg gctccaagct
      781 gagtatcctt ttttcaaaac ggtcaccgac agtgccgtgt ttttcggcaa atctcccacg
      841 accatgggtg ttatcccttt gggggttcca ttgatcgaaa cgctggcacc tgctggtcgt
      901 gaatcaatcc tgagttttcc cagttgcgcc agatttgaat tcgttgtgtt gaaggccgaa
      961 aatgaatcct ttgttttgct ggttgttgta gatgaagaag tcgtttctgt ttgcgatgtg
     1021 ttatgcgggg tggtgatttt ttctgtggga atcaccggaa ttttgttgag ggtcagaccc
     1081 acagggtatt ctttggcggc ggtcaacaca acggcctcta tcgcatcctc ataaccttgt
     1141 tttttgagcg tgatgagaat tttctgtggt atatccagtg cgtcaactgt gtgtggtgtt
     1201 tcgaacgcag ttttttctcc gcccagataa atcgtggccc cttcgggttg agaagaaatc
     1261 accaacgagg cctttggcaa ggccgagagt gtgatgtcga ttgatttatc tccctggtcg
     1321 ttgtttacga tctcctgttc gtttgtaagg tacccgtctt ttgacacggt gatcagatat
     1381 tttttaccaa cctctaatga ggtgatgacg tgaggtgttt gaaaaccaga agggatcccg
     1441 ttgacaagaa tttcggcgcc gccgggattt gagaccacag tcaaagtata ggcgg
//
LOCUS       DATDUN010000058         3751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_541842, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000058 DATDUN010000000
VERSION     DATDUN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541842"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /gene="glpX"
                     /locus_tag="VJC18_00815"
     CDS             <1..823
                     /gene="glpX"
                     /locus_tag="VJC18_00815"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HLD43946.1"
                     /translation="NGMQIDGTVVIGEGERDEAPMLYIGERVGTGQGPKIDIALDPLE
                     GTNLCATGTANSLAVIAAAEDGNFLHSPDTYMDKIAVGPSAKGWIDITKSPTDNLRSV
                     AEAKKCTVQDLTVVILERERHKDLIAEVRENGARIKLISDGDISAAVAACFEATGVDL
                     LMGIGGAPEGVISAAALQCIGGDMQGKLKFRSEEEKERAKKMGITDLNKVYSINDLAS
                     GEVMFAVTGVTNGDFLKGVHFRGNGCTTESIVMRSKTKTIRYLKTEHYFNYKPNY"
     gene            1062..1487
                     /locus_tag="VJC18_00820"
     CDS             1062..1487
                     /locus_tag="VJC18_00820"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLD43947.1"
                     /translation="MVSQSIEIKATPKRCYEVITDYEGYRDFLSSVDDVVVSNKKGNT
                     CEVTYTIKVIKEISYTLKMKATPPSKVEWSLVKSNIMKDNHGHWELEEVKKGVTLATY
                     NVDIKFGLLVPGSVTKILVDKHLPAMLKDFKKRIEVLKK"
     gene            1530..2147
                     /locus_tag="VJC18_00825"
     CDS             1530..2147
                     /locus_tag="VJC18_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HLD43948.1"
                     /translation="MLKKYIAVSGNMGVGKSTLVDFLCRTYDLKPFFEPNDFNPYLED
                     FYKDMPTWAFKSQIFFLSRKFRIHQELGALRTRQPVIQDRTIYEDAEIFATNLYKQKI
                     MCERDFKTYYEMYETILNSLNPPDLMIYLDCSIRTIKKRIRERGRKMELEIPTSYLQR
                     LNHLYEDWVGRYKQSPIIRISTEKLDYISDFIDRDDLMEKIEKYI"
     gene            2209..3372
                     /locus_tag="VJC18_00830"
     CDS             2209..3372
                     /locus_tag="VJC18_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD43949.1"
                     /translation="MIDRLLKLTNHNHSLFIFGPRQTGKTYLIKHTIKPDIFINLLKF
                     TEYQRYLQNPSLLSKEVGSQLNKSGLIVVIDEIQRAPHLLNEVHSILQDHPQVQFILT
                     GSSARKLRKGGVNLLGGRAITTHLYPLTYHETLSNFDLDDTLRFGSLPTIYLEKNTAT
                     KIKLLKAYVNTYLKEEIQQEAMTRNVPAFSLFLELAGFENGHIINFSALSKKIGVHYA
                     TVKEYFQILEDTLLGFYLYPYKKSVRKKIVAHPKFYFFDTGIVSCLQKRLYEPITPAT
                     PPYGLSFEHWLILETQRMLHYHDVEATLSFFRTTDDIEVDLILEIRGQTWAIEIKSSA
                     EPNPSSFKGLKNFLNDHKVDRVLCVCQTPRKYLVDRIEFLPWQEYLNELTVPF"
     gene            3442..3515
                     /locus_tag="VJC18_00835"
     tRNA            3442..3515
                     /locus_tag="VJC18_00835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3476..3478,aa:Arg,seq:tct)
BASE COUNT         1018 a          850 c          925 g          958 t
ORIGIN      
        1 caatggcatg cagattgatg gcacagtggt gattggggag ggcgagcgcg atgaggcccc
       61 catgctctac attggcgaaa gggtggggac agggcagggc cccaaaatcg atatcgcctt
      121 ggacccgctc gaaggaacca acctgtgcgc aacggggacg gccaactcgt tggccgtgat
      181 cgcggcggcg gaggatggta attttctcca ttcacccgat acctacatgg acaaaatcgc
      241 cgtgggtcca tctgccaaag gctggatcga catcaccaaa tcgcccacag ataatttgcg
      301 ttccgtggct gaggccaaaa aatgcactgt gcaggatctc actgtcgtca tcctcgagcg
      361 agagcgtcat aaagacctga tcgccgaggt gcgcgaaaat ggagcccgga tcaagctcat
      421 cagcgatggt gatatttccg cggccgttgc ggcatgtttt gaggcgacgg gcgtggatct
      481 cctcatgggc attggtggcg ctcccgaggg agtcatttca gcggcggcct tgcagtgtat
      541 tggcggcgat atgcaaggca agctcaagtt caggagcgag gaagaaaaag agcgtgccaa
      601 aaaaatgggc atcaccgatc tcaacaaagt ctattcaatc aatgatctgg catcgggaga
      661 ggtgatgttc gcggtcacgg gcgtgaccaa cggtgatttt ctcaagggcg tgcatttcag
      721 gggcaacggg tgcaccacgg aatccatcgt gatgcgctcc aagaccaaga ccatccgtta
      781 tttgaagacg gagcattatt ttaactataa accgaattat taaagcgtgg atcgtggatc
      841 gttaatcgaa aggggtgtca ttccagcgca ggctggaatc cagaattgtc gaaaatatgg
      901 atcccagctt tcgctgggat gacaaacact ggatccctcc ctgcgatcct ttgactttgc
      961 tcaggatggt gagcttgtcg aaccaaggca gggaccacga agggatgaca aaattttttt
     1021 cagttcacga ttaacgatcc acgattcacg attcacgaac tatggtttca caatcaatcg
     1081 aaatcaaggc gacacccaaa aggtgttacg aggtgatcac tgactacgag ggctacaggg
     1141 attttttgag cagcgtcgat gacgtggtcg tgagcaacaa aaagggcaac acctgcgagg
     1201 tgacgtacac catcaaggtc atcaaggaaa tttcctacac gctcaagatg aaggcaaccc
     1261 cgccgagtaa ggtggaatgg tctctggtga agagcaacat catgaaggac aatcacggcc
     1321 attgggagct tgaggaggtc aaaaaaggcg tgaccctggc gacgtacaac gtggacatca
     1381 aattcggttt gctggtgcct ggaagtgtca ccaaaatctt ggtggacaag catttgccgg
     1441 caatgctcaa ggattttaaa aaacgcattg aggtgttaaa gaagtagtgg ccggttgcga
     1501 ccggccatta cgccccagag ggcgcgtgca tgctcaaaaa atacattgcg gtttcgggga
     1561 atatgggtgt gggcaagagc acgctggtcg attttttgtg ccgcacctac gacctcaagc
     1621 ctttttttga acccaacgat ttcaatccgt atctcgagga tttttacaag gacatgccca
     1681 cgtgggcgtt caagtctcag attttttttc tcagtcgcaa attcaggatt catcaggagc
     1741 ttggcgcctt gcgcacccgt caacctgtca ttcaggaccg taccatctat gaggacgccg
     1801 aaatctttgc caccaatttg tacaaacaaa agatcatgtg tgagcgtgat ttcaagacct
     1861 attacgaaat gtacgagacc atcctaaatt ctcttaatcc cccagacctc atgatttatc
     1921 tggattgttc tatccggacc atcaaaaaac gcatccgcga gcggggtcgc aagatggagc
     1981 tcgaaatccc caccagttat ctgcagaggc tcaatcacct gtatgaagac tgggttgggc
     2041 gttacaagca gtctcccatc attcgcattt ccactgaaaa actcgattac atctccgact
     2101 tcatcgatcg cgatgatttg atggagaaga ttgagaagta tatttagcac cctatatttt
     2161 acgtaaaaac aggattgata ttcctataaa tacgactata ataggattat gatagatagg
     2221 ctattgaaac taaccaatca taatcattcg ttatttatat ttggaccacg tcaaacaggc
     2281 aaaacgtatt tgattaagca cacgattaaa cccgacatct ttattaacct attaaagttt
     2341 accgagtacc aacgttattt acagaatccg agtttgctta gcaaagaagt cgggtcgcaa
     2401 ctcaataaaa gtggcctgat tgtggtgatt gatgaaattc aacgggcgcc tcatttgctc
     2461 aacgaagttc actccattct gcaggatcat cctcaggtac aattcatatt gacgggttcg
     2521 agtgccagaa agttgcgtaa gggaggtgtt aatttgcttg gtggtcgggc catcacaact
     2581 cacttgtacc cgctgacata tcatgaaacc ctgtcaaatt ttgatctcga tgacaccttg
     2641 cgttttgggt cactgcccac aatttatctg gaaaaaaaca cagcaaccaa aatcaaattg
     2701 ctcaaggcgt atgtgaacac ctatctcaag gaggagattc agcaggaagc catgacaaga
     2761 aatgtgcctg ccttcagctt gtttcttgaa ttggcaggtt ttgaaaatgg acacatcatc
     2821 aatttcagcg cgctctcgaa aaaaataggt gttcactatg ccactgtgaa ggagtatttt
     2881 cagattctcg aagacacact gctcgggttt tatctttatc cctacaaaaa atcagtaaga
     2941 aaaaaaattg tggctcatcc caaattttat tttttcgata cgggtattgt gtcttgtctg
     3001 caaaaaagat tgtatgaacc catcacaccg gcgacgccac cttatggtct gtcatttgag
     3061 cactggctga ttttggaaac gcagaggatg ttgcattatc acgatgtcga ggcgacactg
     3121 tctttttttc ggacgaccga tgatatcgaa gtcgatttga tcttggaaat tcggggacaa
     3181 acctgggcca tcgaaatcaa atcatccgca gagcccaacc ccagttcatt caaaggtttg
     3241 aaaaactttt tgaatgatca caaggtggat cgtgtccttt gtgtgtgtca gacccccaga
     3301 aaatatctgg ttgatcgtat cgaattttta ccttggcagg aatatttgaa cgaacttacc
     3361 gtgccatttt gaatccattc actgacgtaa aaaaagcaat tgcgtcaggg ggctttcttg
     3421 cagtagagcc gtgtccgcaa tggatccgta gctcagttgg atagagcatc tgccttctaa
     3481 gtagagggtc acaggttcga gtcctgtcgg atccgcagaa tatgatggtc aaaaaaatcc
     3541 cttttcacat attgttgtga aaagggattt tttgtttttg cgcgattatt ttcaagagtc
     3601 tgttaggcgg cctttgcgtt ttgtgtggcc tgttttgccc tgggtttgac gttccaaatt
     3661 ttgatggcca acgcgagtcc tatgattgct ggcaggatca gcgcgatggg ccaatattgc
     3721 cacttcgatg gatccgcctt atccgcgcca g
//
LOCUS       DATDUN010000059         1285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_87098, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000059 DATDUN010000000
VERSION     DATDUN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87098"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VJC18_00840"
     CDS             <1..374
                     /locus_tag="VJC18_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43950.1"
                     /translation="VDKIARIYDYDSDRKSALWQAERKNLDKYHRFFVCLYTYEYKYG
                     DLTSHDTVWELVLDVDGEKIKPEKIELIPKVDPLTQSLFPYSNIWSRHFEVLFPKKNH
                     VAGVPLRLQINGPFGRSVLKW"
     gene            511..1113
                     /locus_tag="VJC18_00845"
     CDS             511..1113
                     /locus_tag="VJC18_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43951.1"
                     /translation="MFKKTLVTVVTLTTVLVAGHVYAQSCGGTIVSDNDCTSNNRPYA
                     ESCCPSGYRVQGVAYNDMNKSDYVDAVSAVCRHVVKGNDMMPTDFQTAPVTHVCEKVE
                     VMSGISCKDMPGKGSGHNSDILDGCTANCMNPTSKQTRMLYSADLAGNGRPYVTHTID
                     LPNRIVGVLYKDGDWKGDYVGGSDRADCATVSYKYQPIVQ"
BASE COUNT          351 a          282 c          317 g          335 t
ORIGIN      
        1 aggtcgacaa gatcgcgagg atctacgatt atgacagcga caggaagagc gcgttgtggc
       61 aagccgagag aaagaatttg gacaaatacc atcgtttttt cgtttgtctg tacacgtacg
      121 aatacaaata tggagatttg acctcacacg atactgtgtg ggagttggtg cttgacgtgg
      181 acggcgaaaa aatcaagccc gaaaaaattg aactcatccc caaggttgat cctttgaccc
      241 agtccctttt tccctatagc aacatttggt cgcgtcactt cgaagtgttg tttcccaaaa
      301 aaaaccatgt ggcaggagtc cctttgcgct tgcagatcaa tgggcctttt ggccgctccg
      361 tcctcaaatg gtgatggggc atctgaaaac agcatctaaa aacttgcttg attgtcacct
      421 gaccattggt tatggagaga gtctgtggga ttttgtataa aaatgccaaa agtatagacc
      481 aatctttttc aatcataagg agaatcagtt atgtttaaaa aaacactagt cactgttgtg
      541 acgctcacga cagttttggt ggcgggccat gtttacgctc aatcatgtgg tggcacaatt
      601 gtttccgata acgattgcac cagcaacaat cgaccctatg ccgaaagttg ctgtccttcg
      661 ggttatcggg ttcaaggtgt ggcctataac gatatgaaca agtccgacta cgttgacgcc
      721 gtttcggctg tttgccgcca tgtcgttaag ggcaacgata tgatgccgac cgattttcag
      781 acagcccccg tgacccatgt ctgcgaaaaa gtggaagtga tgtctggtat ttcctgcaaa
      841 gacatgcccg gcaaaggcag cggacataat tccgacatcc tcgatggctg cacagccaat
      901 tgcatgaacc ccacttcgaa acagaccagg atgttgtaca gtgctgattt ggcaggcaat
      961 ggccgtccct acgtcacaca caccattgat ctccccaacc gcattgtggg tgttctctat
     1021 aaagacggcg actggaaggg cgactatgtg ggtggatcgg atcgtgccga ctgcgccacg
     1081 gtttcataca agtatcagcc tatcgtacaa taacaaggga tgatgatgtt ttttacgaag
     1141 gcgccttctt ttaacgttgt gttaagggag gcgccttttt tttaggctat gggtaacgaa
     1201 ataatgaaga aatgaagcaa tgaagaaatg aaacaatgaa aaagattttg ttcatcaatt
     1261 atgaataccc gcccttgggt ggagg
//
LOCUS       DATDUN010000060         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_336431, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000060 DATDUN010000000
VERSION     DATDUN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336431"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VJC18_00850"
     CDS             complement(<1..1223)
                     /locus_tag="VJC18_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HLD43952.1"
                     /translation="MRTIQFFVVVFFVLAVTACQGPSAKKVNQDYLKQYQDHAGALTI
                     DYYSPTGSILTPQNKIVIHFSQPMVPLTVLESTKQSDLVTITPQIEGYFKWVNSRTLV
                     FQASADLPFATTYKVTVRAGRESLLGFALLKDFEFSFGTPRPDMVTTDPVAGAFDVSR
                     KPVIGVSFNQAVSYQGLTEIVELSYVQGHKVIPTDISCFWGSQAQTQGATQGCTRIEL
                     KPKEMLEKDVDVQLLIRKGLRSQHGPDVSESDKLIQFRTHGDFDLTQIACVGPCTPNG
                     TYLFHATTKIASADFEKFVFFDPPIKGLNNSYKYWSDKDQALSVPLSLQPNTTYQVTV
                     NAELKDVHGQELGQRKTFELKTEHHPAELIVPYVTEQIVSYAPEASLGFDAVNVRSAR
                     ARFKLFGEDSEVVLF"
     gene            complement(1378..1818)
                     /gene="dut"
                     /locus_tag="VJC18_00855"
     CDS             complement(1378..1818)
                     /gene="dut"
                     /locus_tag="VJC18_00855"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HLD43953.1"
                     /translation="MLLKIKRVRDNRDLAMPSYESPHSSGLDIRADVDADVIIGPGKR
                     FLVPTGLAVEFEPGYELQIRPRSGLAIKNGISLVNTPGTIDADYRGEIKIIVINHGET
                     DFIIKRGDRIAQMIMVPVIRAQVIEVDEVGDTVRGAGGFGSTGK"
     gene            complement(1854..>2265)
                     /locus_tag="VJC18_00860"
     CDS             complement(1854..>2265)
                     /locus_tag="VJC18_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43954.1"
                     /translation="HKVPLLGDIPVLGNLFKTRISAKEKTNLLVFITPYIIRERKDYL
                     AILKKKIEERNRFVDMNYGTGQRKHIRNAIINHAESLLEFKCQDAYDEDPCYAAATTT
                     VQDGYPVTTTPITPINTSGEQGTYGPPKKVRYRN"
BASE COUNT          555 a          593 c          556 g          561 t
ORIGIN      
        1 aaaaacaaaa ccacttcaga atcttcccca aacagtttga accttgcccg tgctgagcgc
       61 acattgacgg catcaaaacc cagactggcc tcgggggcat aactcacaat ctgttctgtg
      121 acataaggga caatcagctc cgcgggatga tgctccgttt tgagttcgaa cgtttttctc
      181 tgtcccaact cctgaccatg aacatcttta agctcagcgt tgacagtcac ctgataagtc
      241 gtgtttggtt gcaatgacag gggaacgctc agggcctgat ctttgtccga ccaatacttg
      301 taggaattgt tcagcccctt gatgggaggg tcaaaaaaaa caaatttctc aaaatccgcc
      361 gaggcgatct tggtggtcgc gtgaaaaaga taggtcccat tcggcgtaca ggggccaaca
      421 cacgcgatct gagtcaaatc aaaatcgcca tgcgtcctga actgaatgag tttgtcggac
      481 tcagacacat cggggccatg ctgagaacgc aaccctttgc gaatcaacaa ttgcacatcg
      541 acatcctttt ccagcatttc tttgggtttc agctcaatgc gagtgcaccc ttgggttgcc
      601 ccctgagttt gcgcttggga accccaaaaa cagctgatgt ccgtcggaat caccttgtgc
      661 ccctgcacat aggacagctc aacgatctct gtcagcccct gatatgacac agcctgattg
      721 aaactcacac caatcaccgg ttttcgcgag acatcaaagg ccccggccac agggtctgtg
      781 gtcaccatat cgggacgggg ggtgccaaag gaaaactcaa aatccttgag cagggcgaac
      841 cccaacaggg attccctgcc cgcccgcact gtcactttgt atgtcgtcgc aaagggcagg
      901 tccgctgagg cctgaaatac cagcgtccgc gagttgaccc atttaaaata accctctatt
      961 tgcggtgtga tcgtcaccag atcggattgt tttgttgact ccaataccgt aagaggcacc
     1021 atgggttgcg aaaaatgaat gacaatcttg ttttgaggcg tgaggatact gcctgtgggg
     1081 gaatagtaat caatagtcaa cgcacctgcg tgatcctgat actgtttaag ataatcctga
     1141 ttgacctttt ttgcagaagg cccctgacag gcagtgacgg caagaacaaa aaaaaccaca
     1201 acaaaaaatt gaatggtacg catggaagga acttagcgaa atccgtgacg ggtggcaagc
     1261 actgcgttaa ccctcaaagc gcctaaatac caaagaagaa catataaccg ctagctgtcg
     1321 actgtaagcc actatcgtac gctcgtgagt tcgtgagttc gtgagctcgt aagctcgtca
     1381 ttttcccgtc gacccaaatc cccccgcccc ccgcacagtg tcgccaacct catcgacctc
     1441 aataacctga gcacggatca ctggtaccat aatcatctgg gcaatgcggt caccgcgctt
     1501 gatgatgaag tctgtttctc cgtgattgat cacaatgatt ttgatctctc cccgataatc
     1561 cgcgtcgatg gtaccgggcg tgttgaccag gctgatgccg tttttgatgg ccaagccgct
     1621 acggggacgg atttggagtt catacccggg ttcgaactct actgccagac ctgtggggac
     1681 taaaaatctc ttgcccggtc cgatgatgac atcggcgtcc acatcggctc tgatatcgag
     1741 gcctgaagaa tgaggacttt catatgatgg catggccaaa tcgcgattat cacggacacg
     1801 tttgattttg agtaacatga tgatctccct gccttgagcc ttacgccttt tgcctaattc
     1861 ctatacctca cctttttggg tggcccatag gttccttgct ctccggatgt attgatcggc
     1921 gtgatggggg tcgtggtcac gggatagcca tcctgcactg tcgtggtcgc cgccgcataa
     1981 caggggtctt cgtcgtaagc gtcctggcat ttgaactcca acagggactc cgcatgattg
     2041 atgatggcgt tgcggatatg cttgcgctga cccgtgccgt aattcatgtc gacgaatcgg
     2101 ttacgttctt caatcttctt tttgaggatg gccagataat ccttgcgctc acgaatgatg
     2161 tagggagtga taaaaaccag cagattggtt ttttccttgg cgctgattct cgtcttgaat
     2221 aaatttccca atacggggat gtcgcccagc aagggaacct tgtgc
//
LOCUS       DATDUN010000061         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_252509, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000061 DATDUN010000000
VERSION     DATDUN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252509"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2489
                     /locus_tag="VJC18_00865"
     CDS             <1..>2489
                     /locus_tag="VJC18_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLD43955.1"
                     /translation="SRLSAYYKKADAYEIIKAYKGATLKGWCYEPLFPYFADHAGAFV
                     FLNGDFVTTEDGTGVVHMAPAYGEDDFTACKDAGISLVDPLDSECRFTNLVPDYAGQF
                     CKDADKAIIKRLKDEKKLVHQTTIVHSYPFCDRTDTPLIYRAIDAWYVRVEDLRDKLV
                     KNNAVVHWVPSYVGEKRFDNWLKEAKDWNISRNRFWGSCIPVWIADDKSDMFCVGSIA
                     ELEKLSGQKVTDLHKHVIDQITITKNGKTYRRTPEVLDCWFESGSMPYAQWHYPFEHQ
                     NDFNKLFPADFIAEGLDQTRGWFYTLLVLGTALFDKSPFKNVVVNGMVLAEDGKKMSK
                     RLKNYPDPMTVLKTYGADALRLYMIQSAAVRAEELRFSEAGVKDIVRRILLKWWNAYT
                     FFVSYAVVDGWSQGNELTNSRAHERAMAENTNVIPAILRQAQDGERSRTKAGIQIERN
                     LDSSLRWNDKSIHVSQNILDQWIISRLQTLLKTTEEEMEGYRLYNVVPALLNFIEDLT
                     NIYIRFNRKRFWGEGMTDDKNGAYQTLYDVLYTLAQVMAPFTPFLAETMYHNLKVDDE
                     VESVHLHDYPVSHSELINPDLEASVALVGRVILMGRNVRDQNNIKVKVPLKTLRIIHR
                     DDNLHDRLRSLEGYIKDELNVRVVEYLTNEDDFVELTAKANGAVLGKRLGKKFAEVNK
                     ALQALDSQSLWKLEHGGRLEVVGETLTLQDVMVYRKPKPGHESIVSDAYVTVELDTTL
                     EEEQIWEGLAREVVNRIQKLRKTADLKLSDRIQLEYHTNAEQIRSAITANEHYIKEQT
                     LTVIMTSTADPKGQASEVCEVEGQTITVALR"
BASE COUNT          636 a          593 c          673 g          587 t
ORIGIN      
        1 atcacgcttg tctgcgtatt acaaaaaggc tgacgcctat gagatcatca aagcctacaa
       61 aggggctact ctcaagggct ggtgttacga acccctgttt ccgtattttg ccgatcacgc
      121 gggggccttt gtttttttaa atggtgattt tgtgacgacc gaggatggaa ccggtgtggt
      181 gcacatggcc ccggcatatg gagaggatga cttcactgcc tgcaaagacg ccgggatttc
      241 cctggttgat cctctggact cggagtgccg attcacaaac ctggtgcccg attacgcagg
      301 gcagttttgc aaggatgccg acaaggccat catcaaaagg ctcaaggacg aaaagaaact
      361 tgttcatcaa acgacgattg ttcacagtta tccgttttgt gaccggaccg atacgccgct
      421 catctaccgt gcgatcgacg catggtatgt gcgtgtggaa gacctgcgcg acaaattggt
      481 gaagaacaat gccgttgttc attgggtgcc gtcgtatgta ggggagaagc gttttgataa
      541 ttggctcaaa gaggccaagg actggaacat cagccgcaat cgtttttggg gctcctgcat
      601 ccctgtgtgg attgccgatg ataaatccga catgttttgt gtgggttcga tcgcggaact
      661 tgaaaaactg tctggtcaaa aagtgaccga tctgcacaaa cacgtaatcg atcaaatcac
      721 gatcaccaaa aatggcaaga cgtatcgtcg cacgcctgag gttttggatt gctggttcga
      781 atctggttcc atgccctatg cccagtggca ttacccgttt gagcatcaaa atgatttcaa
      841 caaacttttc cctgcggatt tcattgctga gggtttggat caaacacggg gatggtttta
      901 tacgcttttg gttttgggca cagcgctttt cgataaaagc cctttcaaga acgtggttgt
      961 caacgggatg gtgcttgccg aggacggtaa aaaaatgagc aagcggctca aaaactatcc
     1021 ggaccccatg actgttctca aaacatacgg tgccgatgcg ctccggctct acatgattca
     1081 gtcagccgct gtgcgcgctg aggagctcag attttccgag gctggggtca aggacatcgt
     1141 ccgccgaatt ctgctcaaat ggtggaacgc gtacacgttc tttgtgagtt atgcggttgt
     1201 ggatggttgg agtcagggta acgagcttac gaactcacga gctcacgagc gtgcgatggc
     1261 tgaaaacacg aatgtcatcc ctgcgatcct tcgacaggct caggatggtg agcgaagtcg
     1321 aaccaaagca gggatccaaa tagaaagaaa cctggattcc agccttcgct ggaatgacaa
     1381 atcgattcac gtttcccaaa acatcctcga tcaatggatt atctcccgct tgcagactct
     1441 cctcaaaacc acagaagaag aaatggaggg gtaccgcttg tacaatgtgg tgcccgctct
     1501 actcaatttc atcgaagacc tgaccaatat ttacattcgc ttcaaccgca aacgtttttg
     1561 gggcgaaggc atgaccgacg ataaaaacgg ggcctatcaa accctgtacg atgtcctcta
     1621 cacattggcg caggtgatgg cacccttcac accgttcttg gcagaaacca tgtatcacaa
     1681 tctcaaggtc gatgacgagg ttgagagtgt gcatttgcat gattatccag tgtcccatag
     1741 cgaactcatc aaccctgatc tggaggcttc tgttgccttg gtggggcgcg tgatcctcat
     1801 ggggcgcaat gttcgtgacc agaataacat caaggtgaag gttcctctca aaaccttgcg
     1861 catcatccat cgcgacgaca atctgcatga tcgtctgcgt tccctcgaag gttacatcaa
     1921 ggatgaactc aacgtgcgtg tggttgagta cctgaccaat gaggacgatt ttgtggaact
     1981 cacggccaag gccaatggcg cggtgttggg caaacgtttg ggcaagaaat ttgccgaggt
     2041 caacaaggcc ttgcaggcgc tcgattcaca atctctctgg aagctggaac atggtggtcg
     2101 tcttgaggtt gtgggcgaga ccctgactct gcaggatgtg atggtgtacc gcaaacccaa
     2161 gcccgggcac gagtccattg tctccgatgc ctatgtgact gtggaacttg ataccacact
     2221 agaggaagag caaatatggg aaggtttggc acgcgaggtg gtgaaccgca ttcaaaagct
     2281 cagaaaaacg gcagacctaa agttgtcgga tcgtattcag ctggaatacc acaccaacgc
     2341 tgaacagatc aggtctgcga tcacagccaa tgagcattat atcaaggaac aaacactgac
     2401 ggtgatcatg accagcacgg cagatcccaa agggcaggcc agcgaggtgt gtgaggtgga
     2461 agggcagacg atcacggtgg cgttgaggg
//
LOCUS       DATDUN010000062         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_336854, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000062 DATDUN010000000
VERSION     DATDUN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336854"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VJC18_00870"
     CDS             <1..345
                     /locus_tag="VJC18_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HLD43956.1"
                     /translation="ANYVTGSGNNGECLDKMREELLPASMNKLHLTTVTAYNEDGTFR
                     SRSNYGSAIDFAAPGQNIESLDIDGGMYTTDGTSSATAFVSGIFLVQGFVSSDGTVTD
                     PDGVDISKAHLE"
     gene            787..>2318
                     /locus_tag="VJC18_00875"
     CDS             787..>2318
                     /locus_tag="VJC18_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HLD43957.1"
                     /translation="MCQKPLLLITIICNLCFFSFNAHAAASRTFTVDSTGDESDSSLS
                     DVDCKTAMGRCTLRAAIEQANSLTSSTKIIFKATVITITMGADLPSIEAEIEIEGHDN
                     LTISGDDKYSLSVLSGGQLTVSTVSFVKSYFHNAGTLAIKECSLYGFFGGTAIMNIGE
                     LELNHSKVSHSQGIALNSQNRSTITYSTIDNSNSEYTAVAISCDEPPSDYNSLGMSHS
                     TISDNRGAGISIGENCKADIATSTISHNQGTGGFGGLYNEGTTTLKNVTIANNVACRL
                     YCDSGTIGGGGIIDSSGDGSLALIRTLLVGNQYYDESDNLQNNNCSGVPFEHVSGSIS
                     DDASCKLQDIDTFSQDDLDVKLEALADNGGDTKTHMLGKNSPAIDFGNILSYADLTDD
                     HTDQRGLDLGALYDIGSIDVQDKDTDGALENEDCDDDNPAISPLASETCNGIDDDCDG
                     EIDGASAVDVKHWYEDTDGDNYGNPAVSVRQCFAPAGFDPDNTDCDDNNADANPGETE
                     AC"
BASE COUNT          647 a          486 c          569 g          616 t
ORIGIN      
        1 gccaattacg ttacgggttc aggcaacaac ggcgaatgtc tggacaagat gagagaggag
       61 cttcttccag cctccatgaa caaacttcat ctgacaacgg tgacagctta taacgaagat
      121 gggactttcc gttccagatc aaattatggc tctgcaattg actttgcggc accaggccag
      181 aatattgagt cccttgatat tgatggcggc atgtacacca cagacgggac ctcttctgcc
      241 acagcctttg tctccggaat ttttttggtt caagggtttg tttcgagtga tgggacagtt
      301 accgacccag acggcgttga tatttccaag gcccatctcg agtgagagac attgaatttc
      361 ctcgtctcat tttacttgcc ttgtcaggca aatgttcgac cgagagattt tgacttaccg
      421 aaaaggcagg gctaattgat tctgtaaaat cttacagcac ctggattgcc gctcttccga
      481 tggtcgctcg caatgacggt ttcctgcgag ttcctgtcgt cattcaatag cctttcaggt
      541 ccttaaaacc cctcaaaaaa actatctctg tatggaatag attttgcttg gaaataagca
      601 gttggacagc aaacaacaaa aaggcaagta ataatcagcc cgttgtttct caatacgaga
      661 aaacgggttg tgctcagctt tttttggtgg caagaccagg caaaaattgc ctagggagat
      721 aatcgaagcc tgtttccaca caacgagtgg tcactggagg cggatttgtg cacgaaagag
      781 gtttttatgt gtcagaaacc attattgctc attaccatta tatgtaattt gtgttttttc
      841 tccttcaatg cgcacgcggc agcgtcacgc actttcacag tggatagcac aggagacgaa
      901 tcagatagtt ctttgagcga tgttgattgt aagacagcaa tgggtaggtg cacgttacgg
      961 gcggccattg aacaggcaaa tagtcttact tcatcgacaa aaattatttt taaagcaaca
     1021 gttatcacca ttacaatggg tgccgacctg ccaagcatag aagccgagat agaaattgag
     1081 ggacatgaca atctcacaat ttcgggtgat gacaaatatt ctctctctgt gttgagcgga
     1141 ggtcaattga ccgtttccac ggtctcgttt gtcaaaagct attttcataa tgcggggact
     1201 ctcgccatca aagaatgttc cttgtatggt ttttttggag gcacggctat tatgaatatt
     1261 ggtgaactgg aattaaatca cagtaaggtt tcccacagcc agggcattgc tctgaatagc
     1321 caaaacagaa gtacaataac ttatagtacg attgacaact caaatagcga atacacagct
     1381 gttgccatca gttgtgacga accgccaagt gattataaca gtttgggtat gtctcatagc
     1441 actatcagtg acaaccgagg tgccgggata agcattggtg aaaattgcaa ggcggatatc
     1501 gcaacgagta cgatcagcca taatcaggga acgggcggat ttgggggact ttataacgaa
     1561 ggcacaacga cactcaaaaa tgtgacgatt gccaacaatg ttgcctgtcg cttgtactgt
     1621 gattctggaa caataggagg gggcggcatc attgattcgt caggagacgg ttcccttgcc
     1681 ttgatacgca ctctcttggt cggaaatcaa tattatgatg agtccgacaa tttacaaaac
     1741 aacaattgct cgggtgtgcc gtttgagcat gtttctggct ctatcagcga cgatgcttca
     1801 tgtaaattgc aggacattga tacatttagt caggatgacc tcgacgttaa actcgaggcc
     1861 ttggcagaca acgggggcga cacaaagact catatgcttg gaaaaaacag tcctgccatc
     1921 gattttggga atattttaag ctatgctgat ttaaccgacg atcacacaga tcagagaggt
     1981 ctagatcttg gcgctttata tgatattggg tctatcgatg ttcaggacaa agataccgat
     2041 ggcgcattgg aaaatgagga ttgtgatgac gataatccgg cgataagccc cttggcgtcg
     2101 gaaacatgca acggaattga cgatgactgc gatggagaga ttgatggggc ttctgccgtg
     2161 gatgtaaagc attggtatga agacacagat ggtgacaatt atggaaatcc tgctgtttct
     2221 gtgcggcagt gttttgcccc cgcgggtttt gaccccgata acaccgattg cgatgacaac
     2281 aacgccgatg caaatccggg agaaacagag gcttgtga
//
LOCUS       DATDUN010000063         4126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_169136, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000063 DATDUN010000000
VERSION     DATDUN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169136"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VJC18_00880"
     CDS             complement(<1..778)
                     /locus_tag="VJC18_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43958.1"
                     /translation="MKFFTRLLTTLVTRHPKTVLCFGVLTSCVLLWHASVTLRFDTDQ
                     DHLMSDSEKYYQDYKNFLKEFGDWEYIYVVIKTPATSDGAFLAKKFSKKLSARLESRP
                     DLYNEITTHVDTTKLKNSLLLLADQPTFDHFAKFAGNHEKTFKQFLSVASLSDWYAWI
                     NSLFALARAENNRASIEQFWPLIKNAISGPFDEQALSELNNRSWTSFFAGRVVDPEGY
                     LFSENGKILFVRILPKKDFSRLDIIQEPLAFLRQTIAELKP"
     gene            complement(775..2145)
                     /locus_tag="VJC18_00885"
     CDS             complement(775..2145)
                     /locus_tag="VJC18_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLD43959.1"
                     /translation="MKHVTEIIQSSKESHLKLFDNINPQLGKVLSTIGFDKNFVRAQG
                     ASLFDDKGNEYYDFLAGYGVFAAGRNHPKIKAALKEAIDLDLSSLVQMDAPLLAGILA
                     ERLINMFGHGVRDTVFFTNSGTEANEGALKFAKAATRRDRFLCLEHAFHGLTLGSLSV
                     NGNAEFRNGFGELLPSETVPLNDLSTLEEKLATRQFAAFIFEPVQGKGVYIPEKSYLR
                     EAVKLCHQCGTLVIADEVQTGLGRTGRWLACEHFDVEPDIITISKALSGWFVPVGAIL
                     YKRDIYHQVFSRMDRCVVHSNTFGKNALAMVCALTTLDVIEEEGLLQNAREMGDLLYE
                     KLVLLKNKHDWIKDIRHVGLMFGIEFGRPDSLKKKLTWDTVHKLDKSLFAELIVMPLM
                     SRHRILTQVSGHHQDIVKLLPPLMINETHVTHFVEALDDVLNDCDSITGPVFRMAANL
                     AKHALT"
     gene            2238..3260
                     /gene="tsaD"
                     /locus_tag="VJC18_00890"
     CDS             2238..3260
                     /gene="tsaD"
                     /locus_tag="VJC18_00890"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HLD43960.1"
                     /translation="MPAHKPYILGIDTSCDETSCAILEIGSKKVLSNVVSSQINLHAP
                     YGGVVPELASRSHIENISAVFQGALDEASVMADQIGAVAVTKTPGLIGCLLVGASFAR
                     AFAYRHQIPLFGVNHLMGHLFSPFIDRDPVFPSLGLVVSGGHTNFYLISSFSDIKLLG
                     QTVDDAAGEAFDKAAKMMGLGYPGGPVVDKLAKQGDDRAFGFTIAKVKLGEQYLSFSG
                     LKTAVYHHVRAQTELTQKIKEDLCASLQKSIVETLVRKMAYFVDREGVASLSLSGGVA
                     LNSLLRRRTEGLCDQLKLPGYVAQPRFCSDNGAMIAFAALVSGTQDERLTMSVAPSRK
                     VNAREL"
     gene            3271..3777
                     /locus_tag="VJC18_00895"
     CDS             3271..3777
                     /locus_tag="VJC18_00895"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HLD43961.1"
                     /translation="MLKYFPKKFLELIVKLLKSHSTTQEIADGMAIGVFVSWLPILGI
                     HMPLSFILATLFKKNPWIAMLAAWIVNPLTMLPIYWFNFWVGKQLYHKQLLGFPSDQQ
                     VPAFDFHHIFTASKDILIPLIIGSVVVGVVAAFVSQCLCLRYYDRLKLKFVHFTHHIE
                     AHLHLHKK"
     gene            3777..>4126
                     /locus_tag="VJC18_00900"
     CDS             3777..>4126
                     /locus_tag="VJC18_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HLD43962.1"
                     /translation="MHSHSQSYRPKKSLGQNFLIDRNYQLKIVEAVRADYRQQTILEI
                     GPGRGAITDHLVSFARQLVLIEKDVLLAKALARKYHAQKHVRVIEGDFLNWEGTLPGE
                     VLVVGNLPYNVASQ"
BASE COUNT          949 a          957 c         1156 g         1064 t
ORIGIN      
        1 agggtttgag ttcggcaatg gtctggcgca ggaacgcgag cggttcctgg atgatgtcga
       61 ggcgcgagaa atcttttttg ggcaaaatgc gcacaaagag aatcttgcca ttctcagaaa
      121 acaggtagcc ttcggggtct acgacgcgtc cggcgaaaaa actggtccaa cttctgttgt
      181 tcagctcgct caaagcctgt tcatcgaagg gaccacttat ggcgtttttg atgagcggcc
      241 agaattgttc aatagaggcg cggttgtttt cggctcgcgc tagtgcaaaa agagaattga
      301 tccaggcgta ccagtcggag agtgaggcca cagacaaaaa ttgtttaaat gtcttttcgt
      361 gattgcctgc aaatttggca aagtggtcga acgtgggctg atcagccagg agcaagaggc
      421 tatttttgag tttggtggta tcgacgtgcg tggtgatttc gttgtaaagg tctgggcgcg
      481 actcaagtcg ggcagaaagt ttttttgaaa attttttggc aaggaaggca ccatcgcttg
      541 tagcaggggt cttgatcacc acatagatgt attcccagtc accgaattcc ttgagaaagt
      601 ttttgtaatc ctgatagtat ttttcggaat cagacatcag gtggtcctga tccgtgtcaa
      661 acctcaaggt gacactggcg tgccagagca acacacacga agtcagtacg ccgaaacaga
      721 ggaccgtttt gggatgccgg gtgacgaggg tcgtgagcag tcgtgtaaaa aatttcatgt
      781 cagcgcatgc tttgccaggt tggccgccat gcgaaatacg ggccccgtaa tggagtcgca
      841 atcgttcaag acatcatcca gggcctcgac gaaatgggtc acgtgagttt cgttgatcat
      901 gagtggcggc aggagtttga cgatgtcctg atgatgaccc gagacttgag tgaggatgcg
      961 gtgtcgcgac atgagcggca tgacaatgag ttcggcgaat aaacttttat cgagtttgtg
     1021 cacggtgtcc caggtgagtt ttttcttgag gctgtcagga cgaccaaatt cgatgccgaa
     1081 catcaggccc acatgacgaa tgtctttgat ccaatcgtgt ttgtttttga gcaggacaag
     1141 tttttcgtaa aggagatcgc ccatctcgcg ggcgttctgc aacagtccct cttcctcgat
     1201 gacgtcgagg gttgtcagcg cgcagaccat ggccagggcg tttttgccga aggtgttgga
     1261 gtgcaccaca caacggtcca tgcgggagaa tacctgatgg taaatatcac gtttgtaaag
     1321 gatggccccc acgggcacga accagccgct caaggctttg gagatggtga tgatatcggg
     1381 ttccacgtcg aaatgttcac aggccagcca gcgaccggta cggcccagac ctgtctggac
     1441 ctcatcggcg atcaccagtg taccgcactg atggcatagt ttgacggctt ccctcaaata
     1501 agatttttcg gggatgtaga cgcccttgcc ctgcaccggt tcaaaaataa aggcggcaaa
     1561 ttgtctggtg gccagttttt cctcgagggt gctcaggtcg ttcaggggga ccgtttcgct
     1621 gggcaggagt tccccaaagc catttctgaa ttccgcattg ccattgacag aaagcgaacc
     1681 cagagtgagg ccatgaaagg cgtgctccag acacaagaag cggtccctgc gggttgcggc
     1741 cttggcgaat ttgagggctc cctcattggc ttcggtacct gagttggtga aaaaaaccgt
     1801 gtcgcgaaca ccgtgtccaa acatgttgat gaggcgctct gccaaaatac cggcaagtag
     1861 tggggcatcc atttgcacga ggctggacag atcgagatcg atggcctctt tcagggcggc
     1921 tttgattttg gggtgatttc ggccggcggc gaacacgccg taaccggcga ggaaatcgta
     1981 atactcattg cctttgtcat cgaacaaaga ggctccttgg gcacgcacaa aattcttgtc
     2041 gaaaccgatg gtggaaagca ccttgccgag ctgggggttg atgttgtcga agagtttgag
     2101 atggctttct tttgacgatt gaatgatttc ggtcacgtgt ttcataagtg gctgcgtgaa
     2161 tactggcccg ctcatcaaga gtcaaatacg atattgattc attgcaattt ccggtgtttg
     2221 ccaataaaac gcctcgcatg ccggcacaca agccttacat tctggggatt gatacctcgt
     2281 gtgacgaaac ctcatgcgcc attctggaga tcgggtccaa aaaagttctt tccaatgtcg
     2341 tgtcttcgca gatcaacctc catgccccct atggtggtgt ggttcccgag ctggccagcc
     2401 gttcccatat cgaaaatatc agtgccgtgt ttcagggtgc gttggacgag gcttctgtga
     2461 tggccgatca aattggtgcc gttgcggtga ccaagacacc agggctcatt ggttgtctgc
     2521 tggtgggtgc cagtttcgcc agggcgtttg cttatcgtca tcagatcccc ctttttgggg
     2581 taaatcattt gatgggccat ctgttcagcc cctttattga tcgtgatccc gtttttccga
     2641 gtttgggtct tgtggtgtcc ggtgggcata ccaattttta tctgatctca tcgttttccg
     2701 acattaaact tctggggcaa acagttgatg acgccgccgg tgaggcgttc gataaagcgg
     2761 ccaagatgat ggggctgggt tatccgggtg gtcccgttgt agataagctc gccaaacagg
     2821 gtgatgatcg ggcctttggg ttcacgatcg ccaaggtcaa gctgggcgag cagtacctca
     2881 gtttcagtgg ccttaaaacg gcagtgtatc atcatgtgag ggcgcaaacg gaactgacgc
     2941 aaaagatcaa agaggatttg tgcgcgagtc tgcaaaaatc cattgtcgaa actctggtca
     3001 ggaagatggc gtattttgta gacagggaag gggtggcatc actttccctc agcggtgggg
     3061 tggctcttaa ttcgttattg cgaagaagga cggagggtct ttgtgatcag ctgaaacttc
     3121 ctggttatgt ggcgcaaccc cgattctgct ccgataacgg cgccatgatt gcctttgcgg
     3181 cgctcgtgag tgggactcag gatgaacgcc tgaccatgag cgtggcgccg tcgaggaagg
     3241 tgaatgctcg agagctttga cttagagcct atgcttaaat attttccaaa aaaatttctc
     3301 gaactcatcg tcaaattgct caaaagccac agcaccacgc aggagattgc cgacgggatg
     3361 gccattgggg tgttcgtgtc gtggttaccg attctgggga tccacatgcc tctttccttt
     3421 atactggcca ccttgtttaa aaaaaatccc tggattgcca tgttggccgc ctggattgtc
     3481 aatccgctga ccatgcttcc catctactgg tttaatttct gggtggggaa gcagctttat
     3541 cataaacagt tactgggttt tccctctgac cagcaggtgc cagcgtttga ttttcatcat
     3601 atttttacag cgagcaaaga catactcatc cccctgatca taggcagtgt ggtcgtgggt
     3661 gtggtggccg ccttcgttag tcagtgcctc tgccttcggt actacgaccg attgaagctc
     3721 aaattcgtac acttcacgca ccacatcgag gctcacttgc acttgcacaa aaaatgatgc
     3781 actcacattc ccagtcctat cgccccaaaa aaagtcttgg tcagaatttt ctgatagacc
     3841 gaaattatca actcaagatc gttgaggccg ttcgggccga ttaccgacaa caaacaattc
     3901 tggaaatcgg gccgggcagg ggggcgatca ctgaccattt ggtttctttt gccaggcaac
     3961 ttgttttgat cgagaaggac gttttgttgg ctaaggcatt ggccagaaaa tatcatgcgc
     4021 aaaaacacgt gcgtgtcatc gagggtgact ttttgaattg ggaaggaaca ctgccgggag
     4081 aggttctcgt ggtagggaac ctgccctata atgtggcctc gcagat
//
LOCUS       DATDUN010000064         1095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_44760, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000064 DATDUN010000000
VERSION     DATDUN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44760"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VJC18_00905"
     CDS             complement(<1..707)
                     /locus_tag="VJC18_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HLD43963.1"
                     /translation="MAENNQTNSDESALTPETVPAPPRCRVRDLGVNIGFFSPGKFNA
                     ITDVAGVKVGHATIIEGDGRLVIGKGPIRTGVTAILPNDNIFYDRVVGGGFVLNGAGE
                     VSGFTQVMEWGMIETPILLTNTLSVGTCSEAVVKYMVKRYPGIGQEHDVIIPLVGECD
                     DSWLNDIAGRHVKEEHVQQAIENAKSGPVAEGNVGGGVGMVTCDFSGGIGTASRRVSL
                     VVGTFTVGVLVMSNFGR"
     gene            complement(711..>1095)
                     /locus_tag="VJC18_00910"
     CDS             complement(711..>1095)
                     /locus_tag="VJC18_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HLD43964.1"
                     /translation="VFKNKSEEFHVYSYTHKQAANPHVYYDVPAKIDMALQRNIERVA
                     KNVFTTLGCRDVARVDLRLDEKGTVHFIECNPLPGLTPDWSDLCLAAKAAGMDYRTLI
                     GEIMAPVIKRVKQKSKGAGLLRMVS"
BASE COUNT          253 a          352 c          247 g          243 t
ORIGIN      
        1 atgcgaccaa aattgctcat gaccaaaaca cccacagtga acgtccccac caccaggctc
       61 acgcgacgtg acgccgtccc aatgcctcca ctaaaatcgc aagtcaccat gcccacgcca
      121 ccacccacat tcccctcggc cacgggtccc gatttggcgt tttcgatcgc ctgctgaaca
      181 tgctcttcct taacatggcg acccgcgatg tcgttgagcc aggaatcatc gcactcgccc
      241 accagcggaa tgatcacgtc gtgttcctga ccaatgccgg ggtacctctt gaccatgtac
      301 ttcaccacag cctccgaaca ggtccccaca gaaagcgtgt tggtgagcaa gatgggggtc
      361 tcgatcatgc cccactccat cacctgcgta aaaccgctca cctcacccgc tccgttcaac
      421 acgaagccac cgcccacaac ccgatcgtag aaaatattgt cgttgggcag gatggccgtc
      481 acacccgtac ggatagggcc cttgccaatc accagacgac catcaccctc gatgatagta
      541 gcgtggccaa ccttgacacc cgccacgtcc gtgatggcat taaacttgcc cggggaaaaa
      601 aaaccgatat tcacacccaa gtctcgcaca cgacagcgtg gcggtgcggg tactgtctct
      661 ggtgttagcg cggattcatc gctatttgtc tggttgtttt cagccatgag ttatgaaacc
      721 atccttaata gtccggcgcc tttacttttt tgtttcacac gcttgatcac aggcgccata
      781 atttcgccaa tcaaggttct gtagtccatc cccgccgcct tggcggccag acagagatct
      841 gaccaatctg gcgtcagtcc cggcagaggg ttacattcaa tgaaatgcac agtccccttc
      901 tcgtcgagac gcaggtccac ccgggccaca tcacgacaac ccagtgtggt gaacacgttc
      961 ttggccacgc gttcgatatt cctctgcagg gccatatcga tcttcgcggg gacatcataa
     1021 taaacatgcg ggttagccgc ctgtttatgt gtgtaactgt aaacatggaa ctcttcgctc
     1081 ttgtttttga acaca
//
LOCUS       DATDUN010000065         1221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_337095, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000065 DATDUN010000000
VERSION     DATDUN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337095"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VJC18_00915"
     CDS             <1..934
                     /locus_tag="VJC18_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43965.1"
                     /translation="HKINPVLSLFRAILPGQKIATDKQTIDIMFQPGHVPLLAWGTST
                     SRGDLLDQFHLYGLLIGVLAFFICRGLKFGYPLSLLVTLLCIGLYQVPRFPLTWVLAT
                     LIITLPLSLMKDEQFTRLKTKRFRLNLMILIWACLFITTTVQLTGYSRDRIFSALHPY
                     AGYHPEYDFSEQTAKYDNFASAKRARPMLSRDELKDISTSTEVGAPPEAMPTKGANVE
                     YAQMQLATKEPQNPLEQRDQDSEWTAKPVSVQNSMRGGALVEMTNLNLAPDTDTNVKI
                     LVAGSWLRAFWLLLETVIILSLMVALLTRGRKLL"
     gene            931..>1221
                     /locus_tag="VJC18_00920"
     CDS             931..>1221
                     /locus_tag="VJC18_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43966.1"
                     /translation="MKPIKHFFFLMLLVSVVFPGICFAKLDIAPQTQEALMGILQGLA
                     NDHAYKQWCENHCGQIHEMEFTGTIASGSLNVTIKGHIIGKHNGVLPLFGAVP"
BASE COUNT          293 a          345 c          277 g          306 t
ORIGIN      
        1 tcacaagatc aatcctgttt tgagtctgtt ccgtgcgatc ctcccgggtc aaaaaatcgc
       61 tacagacaaa caaaccatcg acatcatgtt tcagcccggc cacgtccctc tgctggcttg
      121 gggaaccagc acgtcaagag gtgatctgct tgatcaattt catctttatg gtttgctcat
      181 aggcgttctc gcctttttca tttgccgcgg tctgaaattt ggatacccgc tctccctctt
      241 ggtgacctta ttgtgcattg gtctgtatca agtcccccgt ttccccctga cctgggttct
      301 cgcaactttg atcatcactc tgcccctctc cctgatgaag gatgagcaat tcaccagact
      361 caagacaaaa cgtttccgcc taaacctcat gattctcatc tgggcctgcc ttttcatcac
      421 cacaacagta caattgaccg gttattccag agaccgcatc ttcagtgctc tccatcccta
      481 cgcgggatat caccccgaat atgacttcag cgagcagaca gcgaaatacg ataattttgc
      541 ttcggccaag agggccagac cgatgctctc gcgcgatgag ctgaaagaca tatccacttc
      601 gactgaagtg ggtgctcccc ccgaggccat gccgaccaaa ggggcgaatg tggaatacgc
      661 tcaaatgcaa cttgccacaa aggaacctca aaacccgctt gagcaacgcg accaagattc
      721 tgaatggaca gccaaacctg tgagtgtaca aaattccatg cgtgggggtg ccctggttga
      781 aatgaccaac ctcaacctcg cccctgacac cgacaccaat gtgaaaatcc tcgtggcggg
      841 cagttggtta cgcgcgttct ggttactatt ggaaaccgtc atcatcctga gtctgatggt
      901 ggcactactg acacgtggga gaaaactgtt atgaaaccga tcaaacattt ctttttcctt
      961 atgttgctcg tctccgttgt tttccctggg atctgtttcg ccaaactcga catagcgcct
     1021 caaacgcagg aggctctgat gggcattttg caagggttgg ccaacgatca cgcgtacaag
     1081 cagtggtgcg aaaatcattg cgggcagata cacgaaatgg aattcaccgg cacaatcgcg
     1141 tcgggttctt tgaacgtcac catcaaaggt cacatcatcg gcaaacacaa tggtgtcctg
     1201 cctttgttcg gtgctgtccc g
//
LOCUS       DATDUN010000066         1319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_44871, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000066 DATDUN010000000
VERSION     DATDUN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44871"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VJC18_00925"
     CDS             complement(<1..1070)
                     /locus_tag="VJC18_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43967.1"
                     /translation="MYLHHVPPASLWPMSSEGASWPSFESLCEEPCLAQPDGPSLPIR
                     DRFQSIGGRSIGSQRAAATLLASLPFVARDSSGEEGAVSDAFPVKGKSLSEVELSTAV
                     AVEKAKGRTVVCPRCTATNRLERKVHRQFCFECGWKLPLPQEFQRLFADKMCVGVAFV
                     QIHGLDDGGDVASYAPRYYQLIKMAEELCEPYGVSVKFLNGGKLLLVSGLGAIKERPD
                     VNLVRLISVLQTLQVSFRSLASEVTLTASVDCGHCHRDEDDLFGVPLNRAQRQLQTVL
                     KAENPRRQDLAVYVSSAARSVLGSYYQLELFRQGEKYKGFGEALHEISVLGTSFRFLS
                     PRQAIEGERLSAVPRCSRKKTH"
     gene            complement(1125..>1319)
                     /locus_tag="VJC18_00930"
     CDS             complement(1125..>1319)
                     /locus_tag="VJC18_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLD43968.1"
                     /translation="HAVSDNVIEKIFEKGDFYMQMDELKKQVIQRAITRADGNKSQAA
                     RELGLHQVSLYKMAKQLGVE"
BASE COUNT          335 a          379 c          315 g          290 t
ORIGIN      
        1 tgatgagttt ttttacggga acagcgcggc acggcgctca ggcgttcccc ttcaatcgcc
       61 tgtctgggag acaaaaagcg aaaggaagtt cccaaaacag aaatttcgtg cagggcctcc
      121 ccaaaacctt tgtacttttc accctgtcgg aacagctcga gttggtaata ggaacccaga
      181 acgctgcggg ccgccgaact cacgtagacc gccaaatcct gtcggcgtgg gttctcagcc
      241 ttcagcactg tctgtaattg tctctgtgcc ctgttgagcg ggacaccgaa cagatcatcc
      301 tcgtctcgat ggcagtgtcc acaatccacc gaggccgtca gggtgacctc agaagccaga
      361 gatcgaaaag acacctgcaa cgtctgaagg acagagatga gtctgaccaa gttgacatcg
      421 ggcctttcct tgatggcccc caaaccgctc accaacagca gtttgccacc atttagaaac
      481 ttcacactca cgccgtaagg ctcacagagc tcctccgcca ttttgatgag ttgataatac
      541 cggggggcat atgaggccac atcaccacca tcatcaaggc catgaatttg aacaaacgcc
      601 acccccacac acatcttgtc tgcaaacagc cgctggaatt cctgtgggag aggcaatttc
      661 cagccacatt cgaaacaaaa ctgacgatgc actttgcgtt ccaaccgatt ggtcgccgtg
      721 caacgaggac agacaaccgt ccttcccttg gccttctcca ccgcgacagc tgttgataac
      781 tcaacttcgg acaagctctt tcccttcacg ggaaaagcat cagaaacagc cccctcttct
      841 cctgatgaat ctctggcgac aaagggcagg gatgccaaca gagtggcggc ggcacgctgt
      901 gatccaatag acctgccacc gatggattga aaacgatctc ttatgggtaa ggatggccca
      961 tcgggctggg ccaaacaggg ctcttcacaa agggattcga aactgggcca agaagcccct
     1021 tccgaactca tgggccacaa cgatgccggt ggaacgtgat gtaaatacaa tgttgcctcc
     1081 acaaaacaat caagaaatca tgtgccaaaa gtctttgcgg gcacttattc gacccccagt
     1141 tgcttggcca ttttgtacag cgaaacctgg tgcaaaccaa gttcgcgagc cgcctgagac
     1201 ttgttgccat cggcccgggt gatggcgcgt tggatgacct gttttttcag ttcatccatc
     1261 tgcatataaa aatcgccctt ttcaaatatt ttttcgatga cgttgtcgct cactgcgtg
//
LOCUS       DATDUN010000067         2756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_169271, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000067 DATDUN010000000
VERSION     DATDUN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169271"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1157
                     /locus_tag="VJC18_00935"
     CDS             294..1157
                     /locus_tag="VJC18_00935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HLD43969.1"
                     /translation="MKSMTGMGKAKGLVQGSPVRVEIKSVNHRFCEISFRSPGRFSSL
                     EIPAGQFIREQISRGHVDVFVFEEKTPQLSVVEIDAFKNYHDYLSQVARELKLTETVT
                     LQHLLPGVGGWIYKEMDTAVAWQDLRPILNKALKDLISMREMEGSALKSDISDRLLTI
                     ENIKNNVSSLCENVQVELADRLEKKIAEKIQNRSEIDAQRFHQEIVFYLERMDISEEL
                     KRLDSHLKQFKDFLTQDIPVGRKGDFLIQEFNREFNTIGSKSQNAQIAHLVVAAKVEV
                     EKIREQIQNIE"
     gene            1271..>2756
                     /locus_tag="VJC18_00940"
     CDS             1271..>2756
                     /locus_tag="VJC18_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLD43970.1"
                     /translation="MKKEYIVLLICFVALYFSFPVQAFFTEEDYLKKDSELPKPTATD
                     DDLVQKELVDILSHNDDALKVFDSRLAEYLLVKKFYNERNFQLVWGLHKSTNRKIIRE
                     LIKTLEEAYDNGLNPDDYHYSEITKLLDKLNDYGDFARMELLMSDALFKLVQDICNIR
                     VSPKRVHAEWYLPVKKEVAVYPLVAQALEQNTLYRFVSKLYPQTEAYQRLREALIYYR
                     AIEKKGPLPILSGVRKKIVPGDSGPSVYDLRERLRFSGDLHREAQNHIELYDDELKKA
                     VMRYQRLHGLNDDGAVGQFTIDELNRPLAETIKKIRANLEKLRWQSEEELPTKYLYMN
                     IPDSQLFAIENGRVQLQMRTVVGIKTWQTPIFTDEVEYLIINPKWNVPPKILQKEMLP
                     KIQKGHAFLANENYQVRKRENGQFVLVNPNTVDWSQAKPADFQISQKSGRGNALGRIK
                     FIFPNPFDVYLHDTPQKSLFKKDVRTFSHGCIRIERPLELAQYLL"
BASE COUNT          785 a          585 c          669 g          717 t
ORIGIN      
        1 agggtttgaa tgtgttggac ctttatagca agaatggaac ttacgtgaat gggtatgagg
       61 tggagtcatg tcgtttgcgt cacaacgacc tgatcagttt gggcactgcc agtctcaagg
      121 ttagtcttta ttaagtcata agagctcgag atcgagatcg aaatttcgtg ggttcttttt
      181 tttgcgatcc acgattgatg ccctctgggg cacaagtacc ctaaagggca caagtaccct
      241 aaagggcacg agtaccctaa agggcacagg cgatccacga tccactgtta catatgaaat
      301 caatgaccgg tatgggaaaa gccaagggtt tggtgcaggg ttcgccagtt cgtgtcgaaa
      361 tcaaatcagt caatcatcgt ttttgtgaga tcagttttcg ttccccggga cgtttctcct
      421 cattggagat cccggcggga cagttcatca gagaacaaat cagtcgtggt catgtcgatg
      481 tttttgtatt cgaggaaaag acccctcaac tatcagttgt cgaaatagat gcctttaaaa
      541 attaccacga ttaccttagt caggttgcga gagagctcaa actcaccgaa acggtcacct
      601 tgcaacatct tttgccgggc gttggtggat ggatttataa agaaatggac acagcagttg
      661 cttggcagga tttgcggcca atattaaata aggccctcaa agacctgatc tcgatgcgtg
      721 aaatggaggg atcagcctta aagtcagata tctctgacag actcttaacc attgaaaata
      781 taaagaataa tgtttcgtcg ttatgtgaga atgttcaagt tgaactggca gatcgattag
      841 aaaagaagat agcggaaaaa atccagaatc gttccgaaat cgatgcgcag aggtttcatc
      901 aagagattgt gttttatctg gagcgaatgg atatttctga ggaactcaag aggcttgata
      961 gtcacctcaa acagtttaag gattttctga ctcaggacat ccccgtgggg cggaagggtg
     1021 attttctcat tcaggaattc aatcgggagt tcaacacgat cggttccaaa tcacaaaatg
     1081 cccagatcgc tcatctggtt gtggccgcca aggttgaggt cgaaaaaatt cgcgaacaga
     1141 tccagaatat tgaataagta ttccttgacg ttatccaata atatctcata aatacggcca
     1201 aattttgagt gatgaattcc tgtctgacga ccaaagttga atagccttcc tcaaaatatc
     1261 gtacatgatc atgaaaaaag aatacattgt tttgttgatt tgttttgtag ccctctattt
     1321 ttcctttccc gtgcaggctt tttttaccga agaagattat ttgaagaaag attcggagct
     1381 tcccaagccc acggcaacgg atgatgatct ggttcaaaag gaactcgtcg atatcctgtc
     1441 gcacaatgat gatgcgctca aggtgttcga ttcccgattg gctgagtacc tgctggtcaa
     1501 gaaattctat aacgaaagaa actttcagct ggtttggggg ctacacaagt cgacaaatcg
     1561 caaaatcatt cgcgaactga tcaagaccct cgaggaagct tatgataacg ggttgaaccc
     1621 ggatgactat cattattcgg aaatcaccaa gttgctcgac aaattgaatg attatggcga
     1681 ttttgcccga atggaattgc tcatgtccga tgcgttgttc aaactggtgc aggacatctg
     1741 caacattcgt gtgtcaccca agcgggtgca tgccgagtgg tatttgccgg tcaaaaaaga
     1801 ggttgccgtt taccccttgg tggctcaagc cttggagcag aacacgcttt atcggttcgt
     1861 cagcaaactg tatccgcaaa ctgaggctta tcagcgtttg cgtgaggccc tgatctatta
     1921 cagggccatc gaaaaaaagg ggcctttgcc cattctttcg ggggtgcgaa aaaaaattgt
     1981 tcctggtgac agtggcccta gtgtttacga cttgcgtgaa cgtttgaggt tttcgggtga
     2041 tctgcaccga gaggcgcaga atcacattga actgtacgac gatgaactca agaaggctgt
     2101 gatgcggtac cagcgattac acggcctgaa cgatgacggt gccgtgggac aattcacaat
     2161 cgatgagctc aacagacccc tcgccgagac gatcaaaaaa atcagggcga atttggaaaa
     2221 attgcgttgg caaagcgagg aagaattacc gacgaaatat ctttacatga acattcccga
     2281 ttcgcaattg tttgcgattg aaaatgggcg cgttcaattg cagatgcgaa cagtggtggg
     2341 gatcaaaacc tggcagaccc ccattttcac ggatgaagta gagtacctga tcatcaatcc
     2401 caagtggaac gtgcctccca aaatcttgca gaaggaaatg ttacccaaga tacagaaagg
     2461 gcacgccttt ttggccaacg agaattatca ggtgcgcaag cgagaaaatg gtcagtttgt
     2521 attggtcaat cccaacacag tggattggtc tcaagccaag ccagcggatt ttcaaatcag
     2581 tcaaaagtcg ggacgcggaa acgccttggg acgcatcaaa tttatttttc ccaacccgtt
     2641 cgatgtttac ctgcacgaca cccctcaaaa atcactgttt aaaaaagatg tccgaacctt
     2701 tagccacggg tgtattcgta tcgaaaggcc tctggaactg gcgcaatatc ttttgc
//
LOCUS       DATDUN010000068         1308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_542336, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000068 DATDUN010000000
VERSION     DATDUN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542336"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1308)
                     /locus_tag="VJC18_00945"
     CDS             complement(<1..>1308)
                     /locus_tag="VJC18_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43971.1"
                     /translation="AGGKPPQIIDDKAQHKMDPRYFLPDEATRRALTRASAGRDMRPN
                     WVSFTGQAETHKMIKGLPDGLEILCEWNHHRSCYEPVMRNGNMVSVKLAGEDRPMQVV
                     ALTSGTGRTWTLQIPGIPNIFLANFYANESRVSGKPILVVWSTVKGEPHHSYVKKYLH
                     QHDMSPEPTKFLIPEEKDPDNTFAVTHQIGSKRYQLKFRRLTKPDNTQIVVPVCEAQL
                     LGANGEPVQPVPISYYHVYRDHPQVIEVDSQYFEIKLASRPKAEPILELHSTFAGSIR
                     EPSRMDKRVVLADASSSARLLVDMSAQENKFDDELQVALESSQAVHRQFVDAISGDIY
                     EFSFLPDLLPPHLNFALRGVVRLRDGTPEDLGVMTRVVGLGRNQMIYVQLPGNQFVSF
                     PTVPAHTLSITAHGQTLPDHIIASPWKSVEALTPAELNEFTQA"
BASE COUNT          309 a          305 c          358 g          336 t
ORIGIN      
        1 atcgcctgtg tgaactcatt caattctgca ggagtcaatg cttcaacaga tttccaggga
       61 ctggcaatga tgtgatcagg caatgtttgt ccatgtgcgg tgatgctgag ggtgtgtgcg
      121 ggcacagtgg gaaaactcac aaactgattg cccggcagtt gaacgtagat catttgattt
      181 ctgccaagcc cgacgacgcg ggtcatgact ccgagatctt cgggtgtgcc atcccttagt
      241 ctgaccacgc ctcgcaacgc gaagttgagg tggggtggaa gcaggtccgg tagaaaggaa
      301 aattcgtaaa tatcgccgga aatcgcgtcg acaaattgac gatgcacggc ttgtgatgat
      361 tccagagcca cctgcaactc gtcatcaaat ttgttctcct gagccgacat atcaacaagc
      421 agtcttgccg aactactggc atcagcaagg accacgcgct tatccatgcg tgagggttca
      481 cgaatgctcc ccgcaaaagt ggagtgcaac tcgaggatag gctcagcttt gggacgtgat
      541 gccagtttga tttcaaaata ttgactgtcc acttcaatga cttgggggtg gtcacggtag
      601 acgtgataat atgaaatggg cacaggctga acgggttcac cgttagcacc cagcaattga
      661 gcttcacaga caggaaccac gatttgagtg ttgtcgggtt tggtgagcct gcggaatttg
      721 agttgatatc gtttgctacc gatttgatgt gtcaccgcaa aagtattgtc ggggtccttt
      781 tcctcgggaa tgaggaattt tgttggctca ggagacatgt catgttggtg cagatatttt
      841 ttgacatagc tatgatgtgg ttcccccttc actgtggacc agacaaccag aataggtttg
      901 ccggacacgc gggattcatt ggcataaaaa ttggccaaaa agatattggg tatgcccggg
      961 atctgcagtg tccaggtgcg gcctgtgcca gacgtgaggg cgacaacctg catgggtcgg
     1021 tcttctcccg caagcttgac agaaaccatg ttgccattac gcatgacagg ctcgtaacaa
     1081 cttcgatgat gattccattc acacagaatt tcgagaccat cgggtagccc cttgatcatt
     1141 ttatgggttt cagcctgtcc cgtaaaggaa acccaatttg gtctcatgtc gcgccctgca
     1201 ctcgcacgag tgagggccct gcgcgtggct tcatcgggca gaaaatatct aggatccatt
     1261 ttgtgctgtg ccttgtcatc gatgatttgt ggtggtttgc caccagcc
//
LOCUS       DATDUN010000069         1062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_418614, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000069 DATDUN010000000
VERSION     DATDUN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418614"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1062)
                     /locus_tag="VJC18_00950"
     CDS             complement(<1..>1062)
                     /locus_tag="VJC18_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HLD43972.1"
                     /translation="NFMGIEKVELSADFKGDLRVYQQEGVNWLHFLKRFGFGGILADD
                     MGLGKTIQTIAFINQERQTKKLPSLVVCPTSVVENWIRECTRFAPQMKVVALVGKNRS
                     SIFKEAENNDIVITSYPLIQRDLDEYSKRNWYYLIMDEAQKVKNHRTKTHEAFCQIKA
                     KYKLALSGTPIENRLMELWSIFQIVMPGFLMSQTGFKRYWAQPIEKGNNQDRRTELKQ
                     KLMPFILRRTKDQVLKELPAKTETLHYCELTEKQKLLYREIAEYSKSEIFKNIDSKGV
                     EKSYFSILTALLRLRQICCHPSLVNKDMNAPFDESGKIQELFPFLEEVIDEGHRILLF
                     SQFVEMLQIIQTGINQFGW"
BASE COUNT          259 a          265 c          235 g          303 t
ORIGIN      
        1 tgccagccaa actgattaat tcctgtctgg atgatctgca acatttcgac gaactgactg
       61 aagagcaaaa tgcggtgccc ttcatcgatc acctcttcca ggaagggaaa gagctcttga
      121 atcttgccgg actcatcaaa cggggcattc atgtctttgt tcaccagact ggggtgacaa
      181 caaatctgcc gcaaacggag cagagccgtc aaaatggaga agtaactctt ctcgacaccc
      241 ttggaatcga tgttcttgaa aatttcggat tttgaatact cggcaatttc gcgatacaag
      301 agtttttgtt tttctgtgag ctcacaataa tgcaaggttt ctgtcttcgc gggcaattct
      361 ttgagcacct gatctttggt ccgtctcaaa ataaacggca tgagtttttg tttgagttcc
      421 gtacgacgat cctgattgtt gcccttctcg atgggttgcg cccagtaacg tttgaatcct
      481 gtttgcgaca tcaaaaaccc gggcatgacg atctgaaaaa tggaccacaa ttccatcaac
      541 ctgttttcaa tgggtgtgcc cgacagggcc agtttgtatt tggccttgat ctggcaaaaa
      601 gcttcatggg ttttggtgcg atgatttttg actttctgcg cctcatccat gatcaggtag
      661 taccagttgc gtttgctgta ctcgtccaaa tctctctgaa tgagtgggta actggtgatc
      721 acgatatcgt tgttctcggc ctctttgaaa atgctggaac gatttttccc taccaacgcc
      781 acgaccttca tctgtggggc aaaacgagta cactccctta tccagttttc cacaacagac
      841 gtgggacaca ccaccagcga ggggagtttc ttcgtttgcc tttcctgatt gatgaacgca
      901 atggtttgaa tcgtcttgcc caaacccata tcatccgcca gaataccacc gaagccaaac
      961 cgcttgagaa aatgcaacca gttcacacct tcctgctggt acacccggag gtctccttta
     1021 aaatcggcgc tgagttcgac tttttcgatc cccatgaaat tc
//
LOCUS       DATDUN010000070         1297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_211115, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000070 DATDUN010000000
VERSION     DATDUN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211115"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(555..1109)
                     /gene="coaBC"
                     /locus_tag="VJC18_00955"
     CDS             complement(555..1109)
                     /gene="coaBC"
                     /locus_tag="VJC18_00955"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HLD43973.1"
                     /translation="MKNKSKTIVIGVTGGIASYKTCEAVRLLIKEGHAVHVVMTEHAQ
                     KFVTPLTFQTLTGNPVATDMFKLMQESEIGHIKLADSADVVLVCPATANIIAKAAHGI
                     CDDFLSTILLATKAPIIMAPSMNVNMWENPITQDNVNALNKRGVQFVGPDEGVLACGY
                     AGKGRMSEPEKIVLAVNRVVKSSR"
     gene            complement(1114..>1297)
                     /locus_tag="VJC18_00960"
     CDS             complement(1114..>1297)
                     /locus_tag="VJC18_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLD43974.1"
                     /translation="ASEPVFAALFACYFLGESLNRVQWLGGGLIVLAMMLSELKRPVL
                     KMMGRIGAGRDRPSKG"
BASE COUNT          343 a          336 c          268 g          350 t
ORIGIN      
        1 aatgctttgt gatgggggaa acttgcgtgt ctcctgcatg agagactgca tgctgccttt
       61 tttctgttct gtcatggatt ctcctttcag tgagtgtgac aaaattaata attgaaaaaa
      121 accttttcct cagctcacag ccaaaccgcg actacatccc tgtcaatcga caaaaactgc
      181 cgcatcaggc gtggcctgac tactatggga gggcctttta cgccatatga tttatctcag
      241 gcaacctttt tcttgccaca agtatttttt gaccctctcc tgcttctgcc aaaaaaatgc
      301 ttgaccaact aacaacccca agactgagta ttccaaaacc ccttttaccc gcaaacgcaa
      361 gaccaatcaa tattgccaaa acttatccac agaaagtgga taagtcggtt gaacaaaaat
      421 gtcgaacgtg ttttgttttg tgttttagac tataatatcg tcaagttacg tcattttaaa
      481 agattgagcc aaaaagcccc gttttgagaa aaaaaccttg gggtgtggat aacccctatc
      541 tcctgcaaac ccttttatcg gctggatttg acaaccctgt tgacggctaa gactattttt
      601 tctggttctg acatcctccc cttgcccgca taaccgcacg ccaagacacc ttcatcggga
      661 cccacaaatt gaacaccgcg cttgttgaga gcgtttacat tatcttgtgt gatgggattc
      721 tcccacatat ttacgttcat gctcggagcc atgatgatgg gggccttggt ggccagaaga
      781 attgtggata aaaaatcatc acaaatgcca tgggccgctt tggcaatgat attggctgtg
      841 gccgggcata ccagaaccac atcggcagaa tcagccaatt tgatgtggcc aatttctgat
      901 tcctgcataa gtttaaacat atcggtcgcc acaggattac cggtcaatgt ttggaatgtg
      961 agtggggtca caaacttctg tgcgtgctcc gtcatgacca catgaaccgc gtgtccttcc
     1021 ttgatcaaca ggcgcacggc ctcgcaggtt ttgtaggacg caatgccacc tgtcacgcca
     1081 atcacaattg ttttagattt atttttcata tggttatccc tttgagggcc ggtcacgacc
     1141 ggcccctatt cgacccatca ttttcaaaac gggtcttttg agttccgaca gcatcatggc
     1201 cagaacaatc aacccgcctc caagccactg tacgcgattc aggctctccc ccaaaaaata
     1261 gcaggcaaac aacgccgcaa acacaggctc cgaggca
//
LOCUS       DATDUN010000071         3066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_87733, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000071 DATDUN010000000
VERSION     DATDUN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87733"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VJC18_00965"
     CDS             <1..420
                     /locus_tag="VJC18_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43975.1"
                     /translation="VTGVKSLHRAYRSNVFVQKPALHYLLHLGDKTIFYCSDTSYDGS
                     FFFDVGKDQKIDLAILPVDHISPDLVAKKRFMNTRNALQAIQDMGAPKMIPYAFGAFN
                     FASRKADKIVAQLKKDAEQLNLLDRIHVLAPGEEMVL"
     gene            complement(429..1004)
                     /locus_tag="VJC18_00970"
     CDS             complement(429..1004)
                     /locus_tag="VJC18_00970"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLD43976.1"
                     /translation="MQDNINLKFKLDKIVTKVGLAVLALFIIYISYTTLYSNPMKKKL
                     LGNLAPIFQTTSINKTPFNLSDVVGKKVILLNFWASWCPPCRDEVINLNQIGLRVDSP
                     HFAIVALMEDTQPTIEKAREAYSNFNAANPINYPVYVDSDNSIADLYGVKKLPETFII
                     DLGGNILYRHSGPIEDKDRDMFVAMIQQIMY"
     gene            1082..1945
                     /locus_tag="VJC18_00975"
     CDS             1082..1945
                     /locus_tag="VJC18_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HLD43977.1"
                     /translation="MSRHTIKLSSWNVNGIRACIKKGFWDWFDKTEADIVCLQETKIT
                     QKDFEKIAAEYHLISIADTTNEQIVLFDNKPSSLVPRSSSIVYYALATAKKAGYSGVL
                     LLSKIKPRSVTIGLGDPRFDDEGRTIVAHYDDFVFVGCYFPNGGSELERIPYKMQYNE
                     FLLQKLESLREEHKHIVVCGDMNVAHTEIDIKNPKANENNSGFTKTERDWFSQFLSHK
                     YVDVFRHKNPDARHAYTWWSYRPGVRQNNIGWRIDYFVVTEEMLSRVDSTGIQADQMG
                     SDHCPVHLTIN"
     gene            2187..2570
                     /locus_tag="VJC18_00980"
     CDS             2187..2570
                     /locus_tag="VJC18_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011865508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HLD43978.1"
                     /translation="MSSLKKNRASEKKDKNYARALSEHKKRLAGYRERSLALFPWICA
                     HCGREFPADRLRELTVHHKDHNHDNNPNDGSNWELLCIYCHDNEHSTFEDAKWLEQAG
                     PEKVQKSTPMFKPFADLDKKLQEKK"
     gene            complement(2666..>3066)
                     /locus_tag="VJC18_00985"
     CDS             complement(2666..>3066)
                     /locus_tag="VJC18_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLD43979.1"
                     /translation="TGYVFAPYVSHEKIIEDNKPDYYLALRRSQKTFGSARENLGPWL
                     EFFLATTLKQAQMAVALLSAENLESLLTQKQFQVWSYLQQYKEASPRELAQYTGIARP
                     TINQILHKLLGFKKIESYGQGRGTRYRVKI"
BASE COUNT          884 a          700 c          708 g          774 t
ORIGIN      
        1 gttacgggcg taaaatccct gcaccgcgcc tatcgttcaa atgtgtttgt ccaaaaacct
       61 gccttgcatt acttgttgca ccttggtgac aaaaccatct tttattgttc agacacaagc
      121 tacgatggtt cctttttttt cgatgttggc aaggatcaga aaattgatct ggccattttg
      181 cctgttgatc acatcagtcc cgatctggtt gccaaaaaac gtttcatgaa cacgcgaaac
      241 gccctccaag ccattcagga catgggggca cccaaaatga ttccctacgc tttcggggcg
      301 ttcaacttcg ccagccgaaa ggcagacaaa atcgttgccc aattaaaaaa ggatgctgaa
      361 caattaaacc ttttagatcg aatccacgtg ctggcgccag gggaagaaat ggtcctttaa
      421 tggttccctt agtacatgat ttgttggatc atggcgacaa acatgtcgcg gtctttgtct
      481 tcaataggac ctgaatgacg gtagaggatg tttcctccca aatcgatgat gaaggtctcg
      541 ggcaacttct tgactccata aagatcggca atgctgttat cggaatcaac ataaacggga
      601 tagttaatgg ggtttgccgc attaaaattg gaataggcct cgcgcgcctt ttcgattgtg
      661 ggttgtgtgt cctccatgag ggccacaatg gcaaaatgag gtgaatccac cctcagacca
      721 atctgattga gattgatcac ttcatcccgg caagggggac accaactggc ccaaaaattg
      781 agcaaaatga cttttttccc aacaacatca ctcaagttga atggggtttt gttgatcgac
      841 gtcgtttgaa aaatcggtgc caggtttccc aaaagttttt tcttcatggg attggagtac
      901 aacgtggtgt aggagatgta gatgataaac aaggccaaga cagccagacc aactttagtg
      961 acaatcttgt cgagtttgaa cttgagattg atattatctt gcataggaaa aagaccccct
     1021 ttggctggat acgatatttt gagggtttga tccattgaaa aaaacaggtt aacgaatgtc
     1081 catgagccgc cacacaatca aactctcttc ctggaatgtg aatgggatcc gggcctgcat
     1141 caaaaagggc ttttgggact ggtttgacaa gacggaagcc gacattgtct gtctgcagga
     1201 aacaaagatc actcaaaagg atttcgaaaa aatcgcggct gaatatcatc tgatctccat
     1261 tgctgataca accaacgaac aaattgtgtt gtttgataac aagccttcgt ccctcgtccc
     1321 tcgttcatcg tccatcgtgt attacgccct cgccaccgcc aaaaaagccg gctacagcgg
     1381 agtgctcctg ctcagcaaaa tcaaaccgcg atccgtgacc atcggtttgg gcgacccaag
     1441 attcgacgat gaaggccgaa cgattgtggc acactatgac gactttgtct ttgtgggttg
     1501 ttattttccc aacggcggat cagaactcga acgcattccc tacaaaatgc agtacaacga
     1561 attcctattg cagaaacttg aatccctgcg cgaagaacac aaacacattg tcgtgtgtgg
     1621 cgacatgaac gtggcccaca cagagatcga catcaaaaat cccaaagcca acgagaacaa
     1681 ctctggcttt accaaaaccg aacgcgattg gttcagtcag ttcctctctc acaaatacgt
     1741 cgacgtcttt cgtcacaaaa atcccgacgc acgccatgcc tacacctggt ggtcttaccg
     1801 tcccggggtg aggcaaaaca acattgggtg gcgcattgat tattttgtgg tcaccgagga
     1861 aatgctgtcg cgtgttgact ctacgggtat tcaagctgac cagatgggat cagatcattg
     1921 tccggtgcat ttgacgatta attagtcgac aattgtagat cacagattct ttcgagtggc
     1981 aacgagtaca gctcgtattt aaacaaaata caaaagcggt gcggaatctg ggtttatggc
     2041 attcaaagaa atggtaggtt gatatcacaa cggaaatcga gaagaaagat acaatgggta
     2101 tctgtggaca tggataatga tatgtcattg acgcaaccgg aggcaaaaaa tagagagaac
     2161 tgggaacagg aaagttagga ggtgttatgt cgtcccttaa aaaaaacaga gcttcagaaa
     2221 agaaggacaa aaactatgcc agagccctga gtgagcataa aaaacgtttg gccggctacc
     2281 gcgaaagatc cttagcactt ttcccatgga tctgtgccca ttgcggaaga gagtttcccg
     2341 ctgacagact tcgtgaactg acagtccatc acaaagacca taatcacgat aacaacccca
     2401 atgatggcag taactgggaa ctgctctgca tttactgcca tgacaatgaa cactccacgt
     2461 ttgaagatgc gaaatggctt gaacaggcag ggccagaaaa ggttcaaaag agcacgccga
     2521 tgttcaaacc ttttgcggat ctggataaga aattacaaga aaaaaaataa aaccccctca
     2581 gcggcaaagt ttcggttcac acttgtgttc aacctttttg gttggtggat ttaaaaagga
     2641 aatgccccac ttgggggcct tggaatcaaa tcttcactcg gtacctcgtt cctctcccct
     2701 ggccgtagga ttcaattttt ttgaagccca acaacttatg caaaatttga ttgatggtag
     2761 ggcgggcaat tcccgtgtac tgagccaact ctcgcggtga ggcctctttg tactgctgta
     2821 aataagacca aacctgaaac tgtttttggg taagcaggct ttccagattt tctgcggaca
     2881 acaaggccac tgccatttgg gcctgcttga gagtggtggc taaaaaaaac tccagccaag
     2941 gccctaaatt ttcccgggca ctaccaaatg ttttttgaga acgcctgagt gccaaataat
     3001 aatcgggttt gttgtcttct ataatttttt cgtgcgacac atagggggca aagacgtaac
     3061 ccgtct
//
LOCUS       DATDUN010000072         6339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_585108, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000072 DATDUN010000000
VERSION     DATDUN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585108"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VJC18_00990"
     CDS             complement(<1..457)
                     /locus_tag="VJC18_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43980.1"
                     /translation="MLGNQLAKQANVREAALIMVAVLGLAYLAFDYLYAPKKQAVVDL
                     MTEIGTVQEQQESIEKLNKALELKFTKMESDLQQQSQQTVITDPRVLMLKQYRKSQFK
                     NVSDLLQTVMEKTSKSSVTMEAIKHEAPESHQGFFSTPFTLDFYGRFVNI"
     gene            complement(447..1073)
                     /locus_tag="VJC18_00995"
     CDS             complement(447..1073)
                     /locus_tag="VJC18_00995"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HLD43981.1"
                     /translation="MIKRINLIEKKTFALTYLRLVQIGAVMLAITACIIGFQVFNAKR
                     LETKLEASKLVLQELEATRDKLMKTPVKKKVSVGEYQELMDRLEKTPNWSKLISDVTQ
                     KLPNTVWLTSFKSVGGLSVSAATASSPDKGNPKSYRDGDAPPPAETKPQVTGSYKLEV
                     SGLSSAMKNVTEFVAALAQTKALSGIKVDSQKQSFGFSFTLVGDVNAR"
     gene            complement(1070..1993)
                     /gene="pilM"
                     /locus_tag="VJC18_01000"
     CDS             complement(1070..1993)
                     /gene="pilM"
                     /locus_tag="VJC18_01000"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HLD43982.1"
                     /translation="MLNLKKSKAVLGIDIGSKTTKFMQIVFTGTEKPIIQLCKLMPSG
                     TADETFESNLKIFLRDNKIVTPLAACSIDDPSMKIRRMELPKMPEPDMIEAIKWNLRD
                     LVEGDVDHFTVNYSIISENPKDETVPMEIVAYAIDKKVVDSYQLKISQIGFQPFFIEP
                     ASVTLASTLDRCHGADNAYVAGVDIGFKTTLFYVIGKRLFLFSRPMPEISLEAHQKEP
                     ETFNQKLALEIQKSIDTFKVNFKMEEIGTLYLSGGGALLPDLNKYLTMNLGITANTLN
                     PFVTLNPAETLEPGIQPTLFAKAVGLAYLQA"
     gene            complement(2000..2857)
                     /locus_tag="VJC18_01005"
     CDS             complement(2000..2857)
                     /locus_tag="VJC18_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD43983.1"
                     /translation="MSLILDALKKTESSKENSENNDQPSPTLEGSASGTFTMETLSSS
                     ASSAVTLLQSGRNKLIILIAAGILGLSLIIYLAISRGLSELFSFGQKEEVQIVQTPIQ
                     TTTPEPLQEDPARKEQEIIQLKKEAIANFRSAKYLESVEIYKKLVNLLPASPEIYNNY
                     ALSLKKAGKITEAKQTYQIALALNEDYPEGLNNLAAIEISEQKYNEARKHLERAVELS
                     PEYMDAHLHLAICLEKIGDVQKAVLHYQQFLDLSEGKSDRETRLQIENRLSSLTDELV
                     VNSGDGNER"
     gene            complement(2841..3659)
                     /locus_tag="VJC18_01010"
     CDS             complement(2841..3659)
                     /locus_tag="VJC18_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD43984.1"
                     /translation="MYEAFFGFDEKPFTHTPNTKFLYLSEQHEEVLKTLLYGLESRIG
                     FMLLTGEVGSGKTTTIRTLLNIVGSSIETCFIFNPLVNTLDLLRSINRDFGNEWETDS
                     LQKQIDTLNQYLLKLCGEGKTAAVIVDESQNLSFEALEMIRMLSNLETESEKLLNIIL
                     VGQPELEEKLATPQLRQLSQRIQIHVKLKPLGLAETSGYIQHRLNISGPKVAAYFEKT
                     AVKKIFKRSRGIPRLINSICDLSLMAAFCLNTRVIDDKVLSRALREVPSYVAHP"
     gene            complement(4042..4428)
                     /locus_tag="VJC18_01015"
     CDS             complement(4042..4428)
                     /locus_tag="VJC18_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019446488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLD43985.1"
                     /translation="MNNRGFTLIELILVIAILGILAVSAMPKIFSLTTEANTAAASGV
                     AANVLAGITLYGAKQMASTGIESYPAALDAVADATDCTTLNPCFTNVVKDGVTRDWVK
                     LDAARYTHNPTITTYNYNSGTGTFSK"
     gene            4712..5863
                     /locus_tag="VJC18_01020"
     CDS             4712..5863
                     /locus_tag="VJC18_01020"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HLD43986.1"
                     /translation="MPYYQRILNLENDAKKRSLFLFGPRLTGKTALLKKTFPATISFN
                     LLLADVFLKLSQRPQLIRETVSAQGAGRLQPIVIDEIQKMPHLLDEVHNMIEEFGLRF
                     ILTGSSPRKLKRGGANLLGGRAWTKHLFPLVTKEILNYDLQRILNIGALPLVYDSDEP
                     RKDLAAYVGNYLQEEIQAEGLARKIENFSRFLQTAALMNTEVINFESVARDAQVPPRT
                     IREYFMILQDTLIGTLVVPYAKTEKRKTISSAKFYFFDVGVSNFLAGRQNIQPKTELF
                     GKVFEHFIFTELRAFLNYTDKDHSLCFWRTKTREEVDFIIGDEMAIEVKGTEFVADKH
                     LKGLLALNEEMKFKHNIVVSLDSEPRKIGNTDILPVRMFLEKLWSGDFF"
     gene            complement(5884..6267)
                     /locus_tag="VJC18_01025"
     CDS             complement(5884..6267)
                     /locus_tag="VJC18_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017789798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLD43987.1"
                     /translation="MNNRGFTLIELILVIAILGILAVSAMPKIFSLTTEANTAAASDV
                     AANVLAGITLYGAKQMASTGVESYPADLDGLPDETVCSTATPCFTVVVKDGVTRDWRK
                     VNPGWYFHIPTARLFQYIFLDGTFK"
BASE COUNT         1564 a         1336 c         1592 g         1847 t
ORIGIN      
        1 ggatattcac gaaacgccca tagaaatcaa gcgtgaaagg tgtggaaaaa aaaccctgat
       61 gtgattcggg ggcctcatgc ttaatggcct ccatggtgac ggaggatttg gaggtttttt
      121 ccatcactgt ctggagtagg tcagacacgt ttttgaactg ggatttgcgg tactgcttga
      181 gcatgaggac tcggggatca gtgatgacgg tctgttgcga ttgttgctgc aggtcagatt
      241 ccattttggt aaatttaagc tccagggcct tgttgagttt ttcgatagac tcctgctgtt
      301 cctgcacggt tccgatttct gtcatcaggt caaccacagc ctgctttttt ggtgcgtaca
      361 gataatcgaa ggccagataa gccagaccca acacggcgac catgatgagt gcggcttccc
      421 ggacattggc ctgtttagcg agttgattac cgagcattga catctcctac gagagtgaat
      481 gaaaaaccaa atgattgttt ttgggaatcc accttgatgc ccgacagggc cttggtttgt
      541 gccagggcgg cgacgaattc cgtgacattt ttcatggccg aactcaggcc actgacttca
      601 agtttataac ttccggtgac ctgcggtttt gtttctgcgg gaggcggggc gtcaccatct
      661 cgatacgatt ttggattgcc tttatcggga ctactggcgg tggcggcgct gacagacagc
      721 ccccccaccg atttgaaact ggtcagccac acggtgttgg ggagtttctg ggtgacatcg
      781 gagatgagtt tggaccagtt gggtgtcttt tcgagcctgt ccatgagttc ctgatattcg
      841 ccgacagaca cctttttctt gactggtgtt ttcatgagtt tgtctcgcgt cgcttcaagc
      901 tcctgcagga caagtttgct ggcttcgagt tttgtttcca gccgcttggc attgaagacc
      961 tgaaagccga tgatgcatgc tgtgatggcc agcatgaccg cgccaatttg cacaagcctc
     1021 aggtaggtca gggcaaaggt ttttttctca atgaggttga tgcgtttaat catgcttgta
     1081 aataggccaa accaaccgct ttggcaaaca gggtcggttg gatgcctggt tcgagagttt
     1141 ctgccgggtt caatgtgacg aagggattaa gtgtgttggc agtgatcccc aggttcatcg
     1201 tcaggtactt attgaggtct ggaaggaggg caccgccacc agacaggtac agggtgccga
     1261 tttcttccat tttaaaattc actttgaagg tatcaatcga tttttgaatt tcgagtgcca
     1321 gcttctgatt aaaggtctca ggttcttttt gatgggcttc cagactgatt tctggcatgg
     1381 gtcgcgaaaa caggaacagc ctcttgccaa tcacataaaa aagggtggtt ttgaaaccaa
     1441 tgtccacacc ggcgacgtag gcgttatctg cgccgtggca acgatcgagt gttgacgcca
     1501 gtgtcacaga ggctggttca ataaagaaag gttgaaatcc aatctggctg atttttagct
     1561 gataactatc cacgactttt ttgtcgatgg cgtaggccac tatttccatg ggaacggttt
     1621 cgtccttggg gttttcggag atgatcgaat aattcaccgt aaagtgatcg acgtcacctt
     1681 caaccaggtc cctcagattc cacttgatgg cctcgatcat gtctggttct ggcattttgg
     1741 ggagttccat tcttctgatc ttcatggaag gatcgtctat ggaacaagcc gccagcgggg
     1801 ttacaatttt gttgtctctc aggaagattt tgaggttgct ctcgaaggtt tcgtccgcag
     1861 ttcccgatgg catgagtttg cacagctgaa tgatgggttt ttcagtgccg gtgaaaacga
     1921 tctgcatgaa tttggtcgtt ttggaaccaa tgtcgatacc gagcactgct tttgattttt
     1981 ttaagtttaa catgtttcgt tagcgttcgt tgccatcacc actattgacg accagttcat
     2041 ccgtcaagga ggacagccga ttttcgatct gtagcctggt ttcccggtcg cttttgcctt
     2101 cggacaaatc caaaaattgc tggtaatgca atacggcttt ttgaacatca ccaatttttt
     2161 ccagacaaat tgccaggtgc aagtgggcgt ccatgtattc ggggctcagt tcaacggccc
     2221 gttcaagatg tttcctggct tcattgtatt tttgttctga tatttcgata gcggccaggt
     2281 tgttgaggcc ttctggataa tcttcgttga gtgccaaggc gatctggtaa gtttgtttgg
     2341 cttcggtgat tttgcctgct ttttttaaac tcaaggcgta gttgttgtaa atttcgggac
     2401 tggcgggcag gagattgacc agctttttat aaatttccac actctcaaga tacttggcag
     2461 aacgaaagtt ggcgatggcc tcttttttga gttggatgat ttcctgttct tttctggctg
     2521 gatcttcctg gagtggttcg ggtgttgttg tttgaatggg tgtttggacg atttgcacct
     2581 cttctttttg tccaaaactg aacaactccg aaaggcctct gctgatggcc agatagataa
     2641 tgaggctcag acccaaaata ccggccgcga tgaggatgat gagtttgttt ctgcccgatt
     2701 gcaggagggt cacggcagag gaggctgagg aagaaagggt ctccattgtg aaagtgcctg
     2761 acgccgatcc ttccaaagtg ggacttggtt ggtcgttgtt ttcagaattt tcttttgagg
     2821 attctgtttt tttgagggcg tcaaggatga gcgacataac tgggaacctc tctgagagcc
     2881 cggctaagga ctttatcatc gatcacacgg gtattcagac aaaacgccgc catgagactc
     2941 aagtcgcaga tgctgttgat gagtctggga ataccacgag accgcttgaa aatttttttg
     3001 accgcggttt tttcaaagta ggcggccacc ttgggaccgg aaatattgag tctgtgctga
     3061 atgtagccgg atgtttccgc cagacccaag ggtttgagtt tgacgtgaat ctgaatgcgt
     3121 tgcgaaagtt gtctgagttg tggtgtggcc aatttctctt cgagttcagg ttgtccgacc
     3181 agaatgatgt tgagaagctt ttcggattct gtttcgaggt tggacaacat gcggatcatt
     3241 tccaacgctt cgaatgagag gttttggctt tcgtccacga tcacggccgc ggttttgccc
     3301 tcgccgcaca acttcaacag gtactgattg agcgtgtcga tttgtttttg cagagaatct
     3361 gtttcccatt cgttgccaaa atcacgattg atgctacgga gcaggtcgag cgtgttgacg
     3421 agcgggttaa aaataaaaca ggtttcgatg gagcttccta cgatgttgag gagcgttctg
     3481 atggtggtgg ttttgccgga gccgacctcg cctgtgagca acatgaagcc gatgcgggat
     3541 tccagaccgt agagaagtgt tttaagtacc tcctcgtgtt gctccgagag gtataaaaat
     3601 ttggtgttgg gtgtgtgtgt aaatggtttt tcgtcaaacc caaaaaaggc ttcgtacata
     3661 ggctttccac agctttgcgc ccataatgtg caccgttgca ttaacagcag gctgtggata
     3721 actggcccgc acacaaacac attagtggct caaggtcgtg tgactcccct tttgcaacaa
     3781 attcatgtcg ttcctgtgta taatatataa tgaatgaata ataaagtaaa ctgttgaatt
     3841 attttgtgtt ctttagttat ccacagaagg tgttggaagt ctttctcgat ctcatcgaga
     3901 ccaaccagag atcatggagg agctgaagtc gggccaaaca aatatagtat tatcgttgta
     3961 caggagaatg gtgcgatcgg caataaaaaa cccccataca ccaaagtggt gttgggggtt
     4021 ttaaatttta ttcatattag cttatttaga aaaagtaccc gttccagaat tgtaattgta
     4081 tgtcgttatt gtaggattat gtgtgtagcg tgcggcatcg agtttaaccc aatctctcgt
     4141 tacgccgtcc ttcacaacgt tcgtgaagca gggattgagg gttgtgcaat ctgtagcatc
     4201 agcaacggcg tccaaagcag ctgggtagga ttctatgcca gtgctcgcca tttgtttggc
     4261 accgtacagc gtgatgccag ccagtacatt ggcggcaacg ccagaggcgg ccgctgtgtt
     4321 ggcttctgtg gtgaggctga agatcttggg catggcggac actgccaaga tacctaaaat
     4381 agcgatcacg aggatcaatt cgattaacgt aaaaccacga ttattcataa gttacccctt
     4441 tgttttgatt tgtttacact cagcaccagg cccctcctga tattgagtta agcaaaatga
     4501 atactgttat tatacaagag aaagaagttg tcggcaatgg gaatcggtca caatgaattt
     4561 atgacaatat ctgcagcaaa aattaacaat atcaacctgt tcatggtttt gtgggagaaa
     4621 cgtagattat tatcagcaag atacctgctt aatttaatag attttccgca taatacttgc
     4681 ggatttgttt tttttcggtt aaaatgttta catgccttat tatcagagaa tacttaactt
     4741 ggaaaatgac gcaaaaaaac gcagtctttt tttgtttggc ccccgactga caggtaaaac
     4801 ggccttactc aaaaaaacat ttcctgcaac tatttcattc aatcttttgc ttgctgatgt
     4861 ttttcttaaa cttagccaac ggccccaact cattcgcgaa acagtctcag cccaaggtgc
     4921 tgggcgtcta cagccaattg tgatcgatga aatccaaaaa atgcctcatc ttttggacga
     4981 agtccacaac atgatcgaag aatttggttt gcgctttatt ctcacaggaa gcagtccccg
     5041 taaactcaag cgcggcggtg caaatctttt gggcgggcgg gcgtggacca aacatctttt
     5101 tccgctcgtg acaaaagaaa ttctcaatta cgatcttcag cgcatcttga atattggtgc
     5161 gttgccactc gtttatgatt ctgacgaacc gcgcaaagac ttggcggctt acgtgggaaa
     5221 ttatcttcag gaagaaatac aagccgaggg cttggccaga aaaatcgaaa atttttcacg
     5281 ttttctgcaa acagccgctt tgatgaacac ggaggtcatt aattttgaat ccgtagctcg
     5341 cgatgcacaa gttcccccca ggaccattcg cgagtatttt atgatcttgc aggacacctt
     5401 gattggcact ttggtggtac cgtatgcaaa gaccgaaaaa agaaaaacaa taagctccgc
     5461 aaaattttat ttctttgatg tgggggtgag taattttttg gcaggacgcc agaacattca
     5521 accaaaaacg gaattgtttg gcaaggtgtt cgagcatttc atttttacag aactgagggc
     5581 gtttttgaat tacacggaca aagaccattc tctctgcttt tggcggacaa aaacgcgtga
     5641 agaggtggat tttatcattg gcgatgaaat ggcgattgaa gtcaaaggaa ccgagtttgt
     5701 tgctgacaaa catctgaaag ggttgctcgc cttgaacgaa gaaatgaaat tcaaacacaa
     5761 tattgtggtt tctctggatt cagaacctcg caaaattggc aacaccgaca ttttgcccgt
     5821 tcgtatgttt cttgaaaagc tttggtcagg ggactttttt taagcttcaa gtgaatcagg
     5881 tcattatttg aaagtgccat caagaaatat atactggaac agcctagcgg taggtatatg
     5941 aaagtaccaa cctggattga cttttctcca atctctggtc acaccatctt tcacaacgac
     6001 cgtgaagcaa ggtgtggctg ttgaacaaac tgtttcatcc ggaagaccgt ccaaatctgc
     6061 aggataggat tcaacaccgg tgctcgccat ttgtttggca ccgtacagcg tgatgccagc
     6121 aagcacgtta gccgcaacgt cagaggcggc cgctgtgttg gcttctgtgg tcaagctgaa
     6181 gatcttgggc atggcggaaa ctgccaaaat accaaggata gcgatcacga ggatcaattc
     6241 gattaatgta aaaccacgat tattcataag ttaccccttt gttttgattt gtttacactc
     6301 agcaccaggc ccctcctgat attgagttaa gcaaaatga
//
LOCUS       DATDUN010000073         2899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_418907, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000073 DATDUN010000000
VERSION     DATDUN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418907"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VJC18_01030"
     CDS             complement(<1..562)
                     /locus_tag="VJC18_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43988.1"
                     /translation="MLIFIDTARSLPYFSHPVKKILKTVIPLAICVLIFVFIFRKIPP
                     AKAWEALQTSNIPLFIFVAVVYFFWVHVFDCLAIKHFISRFSAPISHKESWLVRGVTY
                     IMMIINYHAAQGVFAYYFKKTHKAPLSKTLGTMAFISIGDLILILTSALIALGFTNVT
                     YQGFDFRLFVLRLAPIIYLGYGLFILF"
     gene            727..1803
                     /locus_tag="VJC18_01035"
     CDS             727..1803
                     /locus_tag="VJC18_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HLD43989.1"
                     /translation="MHEDILNFVAPHLDSKVSPQDISITKLFGQASYREYYRITTPSS
                     LVPRPSSLVLMKLPNGFASPAEEVTKIGEGASKELPFLNVQKYLNTLEIPVPQVLATD
                     EQNGLILLEDLGDTSLEILYRNAGSEFGLVYYKKVVELLIDLQTKTQQNGFGPCVASF
                     RKFDVELLNWEFNHFLEYGIEDRLGITLEENEKRVFRETTAHISSIIAHMPQGFVHRD
                     YQSRNLMFHNMNFTLIDFQDALTGPVLYDLVALLRDSYVCFTPEQTMGLLSHYQQNLP
                     AEHPYHDKWNALLSDFYLITIQRKLKDAGRFQFINTVKKNPGFLPNIPRSLEYVRQAL
                     ETLREMKYDCAELSGFLKKHLENF"
     gene            1872..2114
                     /locus_tag="VJC18_01040"
     CDS             1872..2114
                     /locus_tag="VJC18_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43990.1"
                     /translation="MKKLPDNQVAFYQSPDGSVNIEVLYAEENIWLTQKKMAELFDCS
                     TDNISLHLKNIYLDKEVDENLTAEDFSVVQSEGKQV"
     gene            2324..>2899
                     /locus_tag="VJC18_01045"
     CDS             2324..>2899
                     /locus_tag="VJC18_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLD43991.1"
                     /translation="MNALILAAGFGTRMRPFTNTHPKALVPVNGIPLILYSLAFLKKH
                     GIKNIVINLHHHGRVIKKYLGNGKKIGMTIRYSNEPHILGTGGGIKKALRYLDETTLV
                     INGDILMDFSLTSFLAQHNKKSPLATLLLFQHKNARDFGLIHHNQQSVTSILGSPAPH
                     TKDHSAMFTGIYLITKSLLKKELDKHPANKLF"
BASE COUNT          863 a          700 c          614 g          722 t
ORIGIN      
        1 aaaacaagat gaaaagtcca tagcccaggt aaatgattgg cgccaatctc aatacaaaaa
       61 gcctaaaatc aaaaccctga tacgtcacat tagtaaaacc caacgcgatg agcgcgctcg
      121 tcagaatgag gataagatca ccaatgctga tgaaggccat cgtacccaga gtcttggaca
      181 ggggggcttt gtgggttttt ttaaaataat aggcaaacac cccctgagcc gcgtgataat
      241 tgatgatcat catgatgtag gtcacacccc gcaccagcca gctctctttg tgggagatcg
      301 gcgcagaaaa acggctgatg aaatgtttga tggccaggca atcaaagaca tgcacccaga
      361 aaaaatacac cacagcaaca aaaataaaca gcgggatatt ggatgtctgc agggcttccc
      421 atgccttggc gggtgggatc tttcggaaga tgaagacaaa gatcaaaaca caaatcgcga
      481 ggggtatcac ggttttcaat atttttttca cggggtgcga aaaataggga agcgaccggg
      541 ctgtgtcaat gaatatcaac aagtcttggc aattcgtcgg cgcacagcac gagaattgcc
      601 tttgggtaat tccaccgcag tgcaccctcg tgaacacccg aacacgcctg ccccctgcgg
      661 gggtgaatac ttgaacacac tttctcttgc cttgcgccct gagccttgag cctgtatatt
      721 ccccccatgc acgaagatat tcttaatttt gtcgcccctc atctggattc gaaagtgagt
      781 ccgcaagaca tttcgatcac caaactcttt ggacaagcca gctatcggga atattataga
      841 attaccaccc cctcgtccct cgtccctcgt ccctcgtccc tcgttttgat gaagttgccc
      901 aacgggttcg ccagtcctgc agaagaagtc accaagatcg gtgaaggagc ctcaaaggaa
      961 cttccttttt tgaatgtgca gaaatacctc aacacactgg aaatccccgt ccctcaagtc
     1021 ttggccactg acgaacaaaa cgggttgatc ctgctcgaag atttgggtga cacgtccttg
     1081 gaaatccttt accgaaacgc cgggtctgaa tttggtctgg tctactacaa aaaagtggtc
     1141 gagcttttga tcgacctaca aaccaaaaca caacaaaatg gttttggacc atgcgtggcc
     1201 agctttcgca agttcgacgt ggagcttctc aattgggaat tcaatcattt tctggaatac
     1261 gggatcgaag acaggcttgg catcacgctg gaagaaaatg aaaagcgcgt ttttcgtgag
     1321 accaccgcac acatctcatc aatcattgcc cacatgccgc aaggtttcgt tcaccgtgat
     1381 tatcaatcgc gaaacctgat gttccacaac atgaacttta ccctcatcga ttttcaggat
     1441 gcgctgacgg ggcctgtcct ctatgatctg gttgctttgc tgagagactc ctatgtgtgt
     1501 ttcacacccg aacaaacgat gggactactt tcacactatc aacaaaacct gcctgccgag
     1561 cacccctatc atgacaaatg gaatgctctt ttgagcgatt tttatttgat cacaatccag
     1621 cgcaaactca aggatgccgg tcgttttcag ttcatcaata cggtgaagaa aaatccaggt
     1681 tttctcccca acatcccgcg gagcctggaa tatgttcggc aggcactcga aacgctacgc
     1741 gaaatgaaat atgattgtgc agaattatct ggttttttaa aaaaacattt ggaaaatttt
     1801 tgaaaacaat atgaacgctt atatttcaat cgcggctgat aagctgcaca gttgaaatat
     1861 aatataaaat tatgaaaaaa ctaccggata atcaagtcgc cttttatcaa tcacctgacg
     1921 gctcagtcaa tattgaggta ttgtatgccg aagaaaatat ctggcttaca caaaagaaaa
     1981 tggcagaact ttttgattgc tctacggata atatttcact ccatttgaaa aacatttatc
     2041 tggataaaga agttgatgaa aatctaactg ccgaggattt ctcggtagtt caaagcgaag
     2101 gaaaacaagt ttgattttat gtctcttgag gaatgcagaa catgggcacg aagcctgtta
     2161 accaacgacg ataacgaaca tggtttttat gaggtgccct tattgaaaat aaagaaaatt
     2221 tttacctgcg agttccctgt ttggcgtaat ctgacttgat ggtcacttga aaaaaagttt
     2281 tagccgaaaa aatggtatcg aaattcttgg agagaatgaa acaatgaacg cacttattct
     2341 ggccgctggt tttggcacgc gcatgcggcc gttcacaaat acgcacccca aggctctcgt
     2401 tcctgtgaat ggcatcccgc tgattttgta ctcgctggca tttcttaaaa aacatggcat
     2461 caaaaacatt gtgatcaacc tgcatcatca tggacgcgtc atcaaaaagt atttgggaaa
     2521 cggcaaaaaa atcggcatga ccattcggta ttccaacgaa ccccatattc tgggaaccgg
     2581 cggcgggatc aaaaaggccc tgcggtatct ggacgaaacc acgctggtca tcaatggtga
     2641 tatcctgatg gatttctctt tgacgtcatt ccttgcccaa cacaacaaga aatccccttt
     2701 ggcaacatta ttgctattcc aacacaaaaa cgcgcgtgac tttggtctga tccaccataa
     2761 tcaacagagc gtcacatcga ttctgggctc accagcgccc cacacaaaag atcattctgc
     2821 catgttcacc ggaatttacc tgatcacaaa atcactcctc aaaaaggaac tcgacaaaca
     2881 ccccgccaac aagctgttc
//
LOCUS       DATDUN010000074         2611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_4553, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000074 DATDUN010000000
VERSION     DATDUN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4553"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..998)
                     /locus_tag="VJC18_01050"
     CDS             complement(138..998)
                     /locus_tag="VJC18_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43992.1"
                     /translation="MLKENVLFEIIDSYYGESHKTLEDIARENNLTLKDCEAQWKQIQ
                     QISQKFSQLEELQASQISINKILVVAKEHAQSSLNRVPFWASLLRPAAGFAIVLLTVM
                     SFMVWQHHSTRQGMGNLAQNQGNENHVRTPLQQRLYSTPMDFHTYATPYFSQSGFAQK
                     TPYLNSYVTPVSVSDPNQNTFAIDEELERKMLSKDLNDHEIDTLYFRARRFEKMGYYE
                     DALHDYLFIVQSFPQYNQDDSLTLAIARCHEMMGDKGQAVRVLEGYQKAHPENENTRL
                     WIDLLKSETF"
     gene            complement(1071..1673)
                     /locus_tag="VJC18_01055"
     CDS             complement(1071..1673)
                     /locus_tag="VJC18_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLD43993.1"
                     /translation="MAERDRNLLHLSDEDLFALYQKGNYEAFPVLLARYRKPLYNFLH
                     RLLGNTETADEAFQETFMRVIKSANEYKPTAKFSTWLYTIARNYGIDQKRKERFRNHM
                     SLDDVNDQTANQVDQALSSNAQIAEITTTKELQAHLRAGLNELSSEQREVFVLREFQG
                     LSFDEIGKVTGTSVNTVKSRMRYALTGLQKIFLELGIKLE"
     gene            complement(1666..2184)
                     /locus_tag="VJC18_01060"
     CDS             complement(1666..2184)
                     /locus_tag="VJC18_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HLD43994.1"
                     /translation="MKLSFTSQKIDKIPSEIVVLMLYENDIPFKGILGVLDWRINGRL
                     SEFFKREKFRGKAKELLLIPSESRFKSQEMLVMGLGKKELFEKDHISQVLDYFLDTIE
                     KKKMKQVCFSLSQLFESHFDWRNAVRLLLSKLVDCDSIEEVVLCEPEELINEARRRQI
                     QFGPNIEVVYHG"
     gene            complement(2201..>2611)
                     /gene="nusB"
                     /locus_tag="VJC18_01065"
     CDS             complement(2201..>2611)
                     /gene="nusB"
                     /locus_tag="VJC18_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HLD43995.1"
                     /translation="DAVMGVRRKARELALKAVFEMDFSQIASERVLARIREEHKLDND
                     VYDFLKQLLDAYEVNSLAVNQTIERFSNNWKLSRMASVDRNILRLGVIEIVYLADIPK
                     NVTINEYLEVAKKYGTEESSSFVNGILDKINPTP"
BASE COUNT          662 a          597 c          602 g          750 t
ORIGIN      
        1 gacgataagc aaaaagtccg cagacgtata aggcacgcta cgttgaggac tttttgtgaa
       61 atcggacgca gttgcagacc agagtgtaat tttgcagtag aagatggttt ttagaggtgt
      121 cctaaattgt ggacaggcta aaacgtctcg gacttcagaa gatcgatcca caatctggtg
      181 ttttcgtttt ctggatgggc tttttgatac ccttcgagaa cgcgaacagc ttggcctttg
      241 tctcccatca tttcgtgaca gcgggcaatg gccagcgtga gggaatcgtc ctggttgtat
      301 tgagggaacg actggacgat gaacaggtaa tcgtgcaggg catcttcgta ataccccatt
      361 ttttcgaacc gacgtgccct gaaatacagg gtatcgatct cgtgatcgtt gagatccttg
      421 gaaagcatct ttctctcgag ctcttcgtca atggcaaacg tattctgatt gggatcagac
      481 acactcacag gggtcacata actgttgagg tacggcgttt tttgcgcaaa accactttgc
      541 gagaaatacg gcgtggcata ggtatgaaaa tccatggggg ttgagtagag acgctgttgc
      601 aaaggggtcc tgacgtggtt ttcgttgccc tgattttgag ctagattccc cataccctgt
      661 ctcgttgaat gatgctgcca aaccataaaa gacatgaccg tgagtaacac gattgcaaaa
      721 ccggcggcgg gtctgagtaa agaagcccaa aatggcactc tattaaggct gctttgtgcg
      781 tgttccttgg ccacaacaag aattttgttg atggatatct gagaggcttg aagttcctca
      841 agctgagaaa atttttgcga aatttgttga atctgcttcc actgagcctc acaatccttt
      901 aaagtgaggt tattctctct ggcgatgtct tcgagtgttt tgtgagattc accatagtaa
      961 ctatcgatta tttcgaacaa aacattctct tttagcatca tacctcctgg cattctgatg
     1021 agtggaacaa tgggtaaagt agggttcctg tcagtatcaa cgtttcaaaa tcactcaagt
     1081 tttattccca actcaagaaa tattttttgc aaaccggtca aggcatacct cattctggat
     1141 ttgacagtgt tgactgaggt ccctgtcact ttgccaattt catcaaaaga caggccctga
     1201 aactccctca agacgaaaac ttcccgttgc tcggagctca gctcgtttag cccggcccgt
     1261 aaatgggcct gcagttcctt ggttgttgtg atttctgcaa tctgggcgtt tgaactcagg
     1321 gcctgatcga cctgatttgc tgtctgatca ttcacatcgt ccaaagacat gtggttgcga
     1381 aagcgctctt tgcgtttttg atcgatgccg taattgcggg cgattgtgta tagccaggtg
     1441 gaaaatttgg ccgtgggttt gtattcattg gccgacttga tgacacgcat gaacgtttcc
     1501 tgaaaggcct catcggctgt ctcagtgttg cccagcaacc ggtgtaaaaa attgtacagt
     1561 ggtttgcggt accgtgccaa cagcacagga aacgcttcgt aatttccttt ctgataaaga
     1621 gcgaacaggt cttcatctga aaggtgtaat aagttgcggt cacgctcagc catgatacac
     1681 aacctcaata ttgggaccga actgaatctg acgccttctg gcttcgttga tgagttcctc
     1741 tggttcgcac agcacaactt cttcgatgct gtcgcaatcg acaagttttg agagcaaaag
     1801 tctgacagcg tttcgccagt caaaatgcga ctcgaacaat tgtgacagcg aaaaacaaac
     1861 ctgtttcatt ttcttctttt cgatcgtatc taaaaaatag tccaacacct ggctgatgtg
     1921 atccttctcg aacaattctt tttttcccag tcccatgacc aacatttcct gcgacttgaa
     1981 gcgactctct gaagggatga gcaacaactc tttggcctta ccccgaaatt tttctctttt
     2041 gaaaaactcc gacagtcggc cgttgatgcg ccagtcgagc acacccaaga tgcctttgaa
     2101 gggaatgtca ttttcataca acatcagcac gacaatctct gacgggattt tgtcgatttt
     2161 ctgactggta aacgataact tcatatgcct cgtgaggaag tcagggagtg gggttgattt
     2221 tgtccaggat gccgttcaca aaactgcttg attcctcggt tccatatttt tttgccacct
     2281 caagatattc gttaatcgtt acatttttgg gaatgtcggc caaatagaca atttcgatca
     2341 cacccaatct caaaatgttg cgatcaacac tggccatacg actgagcttc caattattgg
     2401 aaaaacgttc gatggtctgg ttgacggcaa ggctgttgac ctcatacgca tcaagcaact
     2461 gttttaaaaa atcgtacaca tcgttgtcga gcttgtgttc ctcgcgaata cgagccaaca
     2521 ccctttccga tgcgatctgg gaaaagtcca tttcgaacac agcttttaaa gcgagttctc
     2581 ttgccttgcg tctgacaccc atgacagcgt c
//
LOCUS       DATDUN010000075         1718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_253353, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000075 DATDUN010000000
VERSION     DATDUN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253353"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VJC18_01070"
     CDS             complement(<1..553)
                     /locus_tag="VJC18_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43996.1"
                     /translation="MATELLKGQSSSYVDPRVKAVQQEQFDLGDLDGDGKNEFAQLGV
                     TQVSINCAQNESPLSPSITYIDPSMSVGLQSEFAGVLDTFCGEHPTSSVKFDCNPRDF
                     WNQNYEAGDINPSACYAGFSAVGQGYDDPGLNPELRANLPGSLYSSLLPITWSRQTGL
                     INKTISVQEPGNGGKLITGKIIDV"
     gene            complement(574..>1718)
                     /locus_tag="VJC18_01075"
     CDS             complement(574..>1718)
                     /locus_tag="VJC18_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HLD43997.1"
                     /translation="WDSNTPLDAVDPSLRGDRDVVREAVKLTGASLGTAGEPFFSDKE
                     MIYEAFCATPDIYKEAIFNAYIDRFSLTKALERNPLVWQFIPEDLINNRAFVLSALRQ
                     GVPLDQVHPDHLNDPVAIRESIYSKAKPAIDTSLPVFQADPSLLEAYLRSGRELSFDR
                     KDFLLLNRKTMLKPNDQFTTDLQVVALEQYGKQIYPELLTDDERQDREFTVAAAKSMG
                     TDIFAIMPAEFLADKPVIDAAFEGPVSGNAFALLPDALKESPDYANRAVTKNGLALKY
                     TGEGIKANRAVVINAVRENGFALTYASEELKHDVELIGLANGQLGFEYFPKPVTPAPA
                     VSEVVAVAEPLAPSIPASPTVAKPQPKTQTKPQAKTASGAIEDDSY"
BASE COUNT          392 a          414 c          450 g          462 t
ORIGIN      
        1 gcacgtcgat gatcttgcct gtgatcagtt tgccgccgtt gccgggttcc tgaacagaaa
       61 tggttttgtt gatcaaaccg gtttgtctgc tccacgtgat aggcaggagc gaagagtaca
      121 gcgaaccggg cagattggcc ctcaactccg ggttcagacc agggtcatca tagccctgac
      181 caaccgctga aaagccggca tagcaggcac tgggattgat gtctcctgct tcataattct
      241 ggttccagaa atcgcgtgga ttacagtcaa acttgactga gcttgtggga tgctcaccgc
      301 aaaaggtgtc taaaacaccg gcaaactcac tttgtaatcc cacagacatt gatggatcaa
      361 tatacgtgat cgagggcgac agcggtgatt cgttttgtgc gcagttgatg ctcacttgag
      421 tcacaccgag ttgtgcgaat tcgttttttc cgtcaccatc aaggtcaccc agatcaaatt
      481 gttcctgttg aacagccttg acccgaggat cgacatacga cgatgattgc ccttttaata
      541 gttcagttgc cataacatgc tcctttttga ttgttaataa ctatcatctt caatggcacc
      601 gctggcggtt tttgcttgcg gtttggtttg tgtttttggt tgtggtttgg cgacagtggg
      661 tgacgccggt atcgatggcg cgagtggctc tgccaccgcc acaacctcag acacagcggg
      721 tgccggggta actggttttg gaaaatattc aaaacccagt tgaccattgg caagcccgat
      781 cagctctaca tcatgtttga gttcttcgct ggcataggtg agggcaaagc cgttttctcg
      841 cacagcgttg atcaccacag cgcgattcgc tttaatgccc tcgcccgtgt attttaaagc
      901 cagtccgttt ttggtgacgg cgcggtttgc ataatccggt gactctttga gtgcatcagg
      961 aagcagggca aaggcattgc cagacacagg accttcaaag gccgcgtcaa tcacgggttt
     1021 gtcggccaag aattccgcgg gcatgatggc aaaaatatct gtgcccattg atttggccgc
     1081 cgcgactgtg aattcgcggt cctggcgttc gtcgtccgtc agcagttcag gatagatttg
     1141 tttgccgtat tgttccagtg ccacaacctg caggtcggta gtgaactgat catttggttt
     1201 taacattgtc tttctattca gaagcaaaaa atcctttctg tcgaaagaca gctcccttcc
     1261 tgagcgcagg taagcttcca gtagtgaagg gtccgcctga aagacgggaa gacttgtgtc
     1321 aatagcgggc ttggcttttg aataaatgga ttcgcggatg gccaccggat cgttcaaatg
     1381 atctgggtga acctgatcca agggcacgcc ttgtctgagg gcgctcaaaa caaatgctcg
     1441 attatttatg aggtcctctg ggatgaattg ccaaacgaga ggatttcttt ccagcgcctt
     1501 ggtcagagaa aatctgtcaa tataggcatt aaaaatggcc tctttgtaga tgtcaggcgt
     1561 tgcacaaaag gcctcgtaga tcatttcctt gtcgctgaag aatggttcgc ctgctgtgcc
     1621 gaggctggca ccagtcaatt taacggcttc tcgcactacg tctcgatctc cccgtaacga
     1681 gggatccaca gcatccaatg gggtgttgga atcccagt
//
LOCUS       DATDUN010000076         4889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_460174, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000076 DATDUN010000000
VERSION     DATDUN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460174"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VJC18_01080"
     CDS             <1..493
                     /locus_tag="VJC18_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD43998.1"
                     /translation="EARKLKSSIGRVSTPDRGVGFDAIFIPDSYQNVNAIVGALEFNG
                     LSGVALLGTNAWNDPKLSPQIAAKFPGSFFVDLYDPQATTGTVDRFNSLFTASFGRAP
                     RVLEAYGYDIMMMIREIASDRGSDNIRNAILEGRGYNGVTGIKGFSAGQGPIIESRVM
                     MIK"
     gene            590..1600
                     /gene="thiL"
                     /locus_tag="VJC18_01085"
     CDS             590..1600
                     /gene="thiL"
                     /locus_tag="VJC18_01085"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HLD43999.1"
                     /translation="MKLNEFDLIKNIQTHLVSTNHNLLQGIGDDCAVINKDEQTAFVI
                     STDALIEDVHFDLKNYSYVELGKKAMAVNLSDIAAMGAKPLYAFITLGIPSKCVADNI
                     SDLYIGLEQVAQEFSVAIAGGDLSRSSHHLFLSLTVVGEVAKNGCKLRSGAQAGDGIY
                     VSGLLGSAAIGLSGLNKKRKLETHYLNAIKNPRPKIPLANILSECPYVHSLIDISDGL
                     VQDLGHVMQASHRIAKIELDKIPCEPDFYDTCKKFRLNGHETLLTGGEDYQLLFTMND
                     DELGDLLKKLEMKPNIKITRIGSVLQDVSNKDQNTQCVLILDEQGKEIKLGKTGFEHF
                     SS"
     gene            1597..2274
                     /locus_tag="VJC18_01090"
     CDS             1597..2274
                     /locus_tag="VJC18_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44000.1"
                     /translation="MKTQIIPIIIFALLITTRPVVAKPPSVCPCATSAQLWELAKGRS
                     IASQTEPDFRKMGQLAADGVYFAELCLKKNPKAAGCYYYRAINRGLELKTRTSGIKVR
                     LTKMVQDLQKTIQLDERYDEGGAYNALGYIFLKTPALSHFGPELQRDLTKAEHYAQKA
                     LKISPNNPYHLTLAGEIAIKRQAFDQALAYFRKANSELAKITKPSELDEQLKKDLKKF
                     IKKINNR"
     gene            2474..2929
                     /locus_tag="VJC18_01095"
     CDS             2474..2929
                     /locus_tag="VJC18_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLD44001.1"
                     /translation="MASLNKVMLIGNLGQDPETRYTPSGQAVANFSIATTEKWNNKQS
                     GQPEEKTEWHRIVVWGKLAELCREYLAKGRTVYIEGRLQTRSWDDKDGNKRYTTEIVA
                     QTVQFLSKREGGQPSYQAPSAQTAPQTAGTPSSQDAGPPPFDSDDDIPF"
     gene            3086..3826
                     /locus_tag="VJC18_01100"
     CDS             3086..3826
                     /locus_tag="VJC18_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44002.1"
                     /translation="MEKLFKLRTKDGHNIHGILNFKNRLPNKCVIFVHGLTGKPNNHW
                     FYNAKNYFVQKGYAVIRFYLYTDLPQARQLDQCTLAIHSQDLNQVIDYARRKGFKSLS
                     LVGHSLGGPTILNAKTKNLSSLVFWDPLFNPQQFIKDVAIFDKQMNAYILKWGVTHIL
                     GKPMYQELCNVTHYKTALKENMIPIKIIAAGKGFFLKDCRQYYQHARSQKSLVVIAGA
                     THCFDEEGTASKLFKESHEWIAKHQPNH"
     gene            complement(3846..4721)
                     /locus_tag="VJC18_01105"
     CDS             complement(3846..4721)
                     /locus_tag="VJC18_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HLD44003.1"
                     /translation="MFFPRYSDVQLLEVYGLTGGVPKYFELIDDHAPVLKNIEQTIRE
                     RTTMLTSEIYFLLHEEFREIRVYLAILRALGKKVSTLGELSMFSGLESKVVSKYVDDL
                     VQLKIIGRQIPAGEDCQKSRRSQYVLRDLFLNFYFHFIAPFLQDIDKARFEGVLKNLH
                     QQFDAYMGKFVFERVCQDHLARLGDEGKLSFRPTTVGAYWDKDCQIDILGINHHDRVM
                     IAGESKWTNKKMGLSVLKELETKVKKLSRKYDYFCHLVLFSRSGFSDDLVDTAKKQNA
                     LLVALKDISLPKTTL"
BASE COUNT         1422 a         1230 c         1086 g         1151 t
ORIGIN      
        1 cgaggcccgc aaactcaaga gcagcattgg ccgcgtgagc acgcccgatc ggggagttgg
       61 cttcgacgcc atcttcattc cggacagcta ccagaacgtc aacgctattg tgggtgccct
      121 cgaatttaat gggctctctg gagtggccct gctggggacc aacgcctgga atgaccccaa
      181 actttcccct caaattgccg cgaaatttcc gggatcattt ttcgttgacc tctatgatcc
      241 gcaggcgaca acagggacag ttgacaggtt taattccctg ttcacagcca gttttggacg
      301 cgccccccgc gtgctagagg cttatgggta cgacatcatg atgatgattc gcgaaatcgc
      361 ctcagacagg ggaagcgaca atatccgcaa cgccatcctg gaggggcgag ggtacaatgg
      421 ggtcacgggc atcaagggtt tttccgcagg acaaggaccc atcatcgaat cgagggtgat
      481 gatgatcaag taatgcaaca atgaaacaat gcaacaatga aacaatgaag taatgaaaaa
      541 atgtattgtc ctgtgcacgt gaacacatga acacatgaac acatgaacta tgaaactcaa
      601 cgaatttgac cttataaaaa atatccaaac tcatctggtg tctaccaatc acaatctcct
      661 tcagggcatt ggcgatgact gcgccgtgat caacaaggac gaacaaaccg cgttcgtcat
      721 ctctacagac gccttgattg aagatgtcca tttcgatctc aaaaattatt cgtacgttga
      781 acttggcaaa aaggccatgg ccgtgaattt aagcgatatt gccgcgatgg gggccaaacc
      841 cctttacgcc tttatcactt tgggcatacc gtcaaaatgc gtggccgaca acatctccga
      901 cctgtacatc gggttggaac aggtggccca ggaattttcg gtggcgattg ctggcggtga
      961 tttatcccga agttcccacc atctgttcct gagtctcacg gtcgtgggtg aagtggccaa
     1021 gaatggttgt aagctcagat caggcgccca agcgggagac gggatttatg tttccggtct
     1081 gttggggtcg gcggccattg gtctttcggg actaaacaaa aaacgcaaat tggaaacaca
     1141 ttaccttaat gccatcaaaa acccgcgccc caaaatccct ctggccaata ttttatcgga
     1201 gtgtccctac gtccactcac tcatcgatat cagcgacggt ttggtgcagg acttgggcca
     1261 cgtgatgcag gcttctcacc ggattgccaa gattgaactg gacaagattc cctgcgagcc
     1321 cgatttttac gatacgtgta aaaaatttcg tctgaacggt cacgaaacac tgctcaccgg
     1381 aggcgaggat tatcaattac tgttcaccat gaacgatgac gaactgggcg accttctcaa
     1441 aaaattggaa atgaaaccca atatcaagat cacgcgcatt ggttctgtgt tgcaagatgt
     1501 ctctaacaaa gatcagaata cacaatgcgt cctgattttg gatgagcagg gaaaagaaat
     1561 caagctgggc aaaactggct tcgaacattt ttcttcatga aaactcaaat catccccatc
     1621 atcatttttg ccttgctcat caccacccgc cctgttgtag ccaagccccc cagcgtttgt
     1681 ccctgtgcga caagcgcaca actatgggaa ctcgccaaag gacgcagcat tgcctcccaa
     1741 acagaaccag attttcgcaa aatggggcaa ctggccgcag acggcgttta ttttgcagag
     1801 ctctgtctca aaaagaatcc aaaagcagcc ggatgctatt actaccgcgc catcaaccga
     1861 ggactggagc ttaaaacacg aacttccggg atcaaggtga gactgaccaa aatggtgcag
     1921 gatctgcaga aaacaattca actcgacgag cgctatgacg aaggtggcgc gtacaatgcc
     1981 ttgggttaca tttttttaaa aacccctgcc ctgtcccatt ttggtcccga actgcaacgc
     2041 gatctgacca aggcggagca ctacgcccaa aaagcattaa aaatctctcc caacaaccct
     2101 tatcatttga cattggcagg cgaaatcgcc attaagcgac aggcctttga tcaggcactg
     2161 gcctatttca gaaaagccaa ttccgagctt gcaaaaatta cgaagccctc ggagctggac
     2221 gaacaactga aaaaagattt aaagaagttt attaagaaaa ttaacaaccg ctgatctctg
     2281 atctgaggtt acaccagtcc caaaatcttc catgatgttt ttttgggtga tatggggagc
     2341 tgttattttg atgcggcctg cgtagcgagc ctgcgagcgg agagcatcaa aaaatagcga
     2401 cccatagcag gaccccaaaa aagagtcatt agagtttttg ggactggtgc tattcgcaaa
     2461 gaaaggacgc tccatggcaa gtttgaataa agtcatgctc atcggcaatc tgggccaaga
     2521 tccggaaaca cgctacaccc ctagcggaca ggctgtagcc aatttcagca ttgccaccac
     2581 agaaaaatgg aacaacaaac aatccggtca gcccgaggaa aaaaccgagt ggcaccgcat
     2641 tgtggtgtgg ggcaaactgg cggaactctg ccgggaatac ctcgccaagg gccgtaccgt
     2701 ctacatcgaa gggcgtctgc agacacgcag ttgggacgac aaggacggca acaagcgtta
     2761 caccaccgag attgttgccc agaccgtgca atttttgagc aaacgcgaag gcgggcaacc
     2821 atcctatcag gcaccctctg cccaaaccgc tccgcagacc gctgggactc catcctcgca
     2881 ggatgccggt cccccgccgt tcgacagcga cgacgacatt ccgttttaaa taacaaaaca
     2941 acccgcgttc ctcaccaaat atgataagaa atgcgggtca gctgggatca cgctttatcg
     3001 tggatcacta cgttaccatg tgacaggatt cttggcgcaa tgcgccacac atgcctttgg
     3061 gcatgtgcgt cgcaatgcac cccctatgga aaaactattc aaactcagaa caaaagacgg
     3121 ccacaacatt cacggtatac tcaatttcaa gaatcgtctt ccaaacaaat gcgtcatttt
     3181 tgtacatggt ctcacgggca agccgaacaa ccattggttc tataatgcca aaaactactt
     3241 cgttcaaaaa ggttacgcgg tcattcgttt ttatctctat accgatcttc cccaagcgcg
     3301 ccagctcgat caatgcacgc tcgcaatcca tagtcaggac cttaatcaag tgattgatta
     3361 cgcgcgacgt aaagggttta aaagtctttc tttagttggc cacagtctgg gcggccccac
     3421 catattgaac gccaagacaa aaaacctttc atctcttgta ttttgggacc cactttttaa
     3481 cccccaacaa tttatcaaag atgtggccat atttgataaa cagatgaatg cctatatcct
     3541 caaatggggc gtgacgcata ttctgggaaa accaatgtac caagagttat gcaatgtcac
     3601 ccattataaa accgccctca aagagaacat gatccccatt aaaattattg ccgctggcaa
     3661 gggattcttc cttaaggatt gtcgacaata ttatcagcat gcccgctcac aaaaatcact
     3721 tgtggtgatt gcgggcgcga ctcactgttt tgacgaggaa ggcacagcct caaagctatt
     3781 caaagaatct catgagtgga ttgcaaaaca tcaaccaaac cattaacccg tgcttttcgg
     3841 ctatttcaca aagtggtctt aggcaagctg atgtctttga gcgcaaccag caaagcattc
     3901 tgttttttgg ctgtatccac caagtcatcc gaaaaaccag agcgactaaa caagacgagg
     3961 tgacaaaaat aatcgtattt gcgtgacaac ttcttcacct tggtttcaag ttccttcagg
     4021 acagacaacc ccattttttt attcgtccac ttggactcac ccgcaatcat cactcggtcg
     4081 tggtgattga tgcccaaaat atcgatctgg caatccttgt cccagtaagc accaacggtc
     4141 gtgggtctga agctcaattt cccctcatcg ccaagacgcg ccagatgatc ttgacaaaca
     4201 cgttcaaaaa caaattttcc catgtacgca tcaaattgtt gatgaagatt tttaagaacc
     4261 ccttcaaatc gggccttgtc aatgtcttgt aaaaacgggg caatgaaatg aaaataaaag
     4321 ttcagaaaca ggtcccgtaa aacatattgg cttcgacgac ttttttgaca atcctcaccg
     4381 gcagggatct gtctcccgat tattttcagt tggacaagat catccacata ttttgaaaca
     4441 accttgcttt cgagaccact gaacatggag agctcaccca gcgtgctaac tttttttccc
     4501 agcgctctga gtatggcaag gtagacgcga atttctcgga attcttcgtg caatagaaaa
     4561 taaatttctg acgtcagcat tgtcgttctt tcacgaattg tttgctcaat atttttaaga
     4621 acaggggcat gatcgtcaat gagctcaaaa tatttgggaa cacctcccgt gagaccataa
     4681 acctccagga gttgtacgtc actgtatctg ggaaaaaaca cggcgagttc actcagggga
     4741 attggttcaa cccaaaacgt gcagttgaaa acttaagatg aaaaaaatgc ctccttaagg
     4801 caaagggatt attggattta cgagaaacaa tgatcccaaa gcaaaaggag gcaaaaagtg
     4861 aattacgaaa acggcaataa gccaatttt
//
LOCUS       DATDUN010000077         1159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_211186, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000077 DATDUN010000000
VERSION     DATDUN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211186"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1159)
                     /locus_tag="VJC18_01110"
     CDS             complement(<1..>1159)
                     /locus_tag="VJC18_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016210187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HLD44004.1"
                     /translation="ESQVRKAKQLGFSDKVLGRFKNLSEQDFRTERLKLGVYPVTKQI
                     DTLAGEFDAETNYLYMTYHGVSHDIKPSIDPMVMILGSGPYSIGSSVEFDWSGVTTAR
                     TLRKLGKKTILINSNPETVSTDYDESDRLYFEQLTFERVQDIADFEHHQGIIVSVGGQ
                     IANNLVMRLSNRGYKILGTSADHIDQAENRERFSQLLNQLEIDQPAWTTALSLDAAKI
                     FAKQVGYPVLIRPSFVLSGAAMNVIWSEDELEIYLHLATDVSPDHPVVVSDFITHAKE
                     LEMDGVTDQGKIIIYAMSEHIENAGVHSGDATIVTPPQRLYLETIRRTKKITQQIVKA
                     LNITGPFNIQLIAKNNAIQVIECNLRASRSFPFVSKVTGHNFIEIATQVLMG"
BASE COUNT          308 a          277 c          258 g          316 t
ORIGIN      
        1 ccccatcagc acctgggtcg cgatctcaat aaaattatgc cctgtgactt ttgagacaaa
       61 agggaaagat cgcgaggccc taagattaca ctcgatgacc tgaatcgcat tgtttttggc
      121 aatcaactga atattgaaag gccccgtgat attgagcgct ttgacaatct gttgggtgat
      181 ttttttggtt ctacggatgg tctctaaata taatctttgg ggcggggtaa caatcgtggc
      241 atctcccgaa tgaacgcccg cattttcaat atgctccgac atggcataga taataatttt
      301 accctgatcc gtcacaccat ccatttcaag ctctttggca tgggtgataa aatcagagac
      361 gacaacaggg tgatctgggg agacatcggt cgctaggtgc aaatagattt ccaactcatc
      421 ttcgctccaa atcacgttca tagcagcgcc agataagaca aacgatggcc ggatcaacac
      481 tggataaccc acctgttttg caaatatctt tgcagcatcc aatgaaaggg ctgttgtcca
      541 ggcaggctgg tcaatctcca gttgattcag caattgtgaa aatcgttccc ggttctccgc
      601 ctgatcaata tgatccgcgg aggtacccaa gattttatac ccgcgatttg acagtcgcat
      661 cactaagtta ttggcaatct gaccgccgac agacacaata ataccttgat gatgctcaaa
      721 gtctgcaata tcctgaacac gctcaaaggt cagctgctcg aagtagagtc ggtctgactc
      781 atcgtaatcg gtcgagacgg tttcagggtt ggagttgatc aaaatggttt tttttcccag
      841 ttttcttaag gtgcgagccg tggtgacccc gctccaatca aactcaaccg atgagccaat
      901 gctataaggg cctgagccta gaatcatcac cataggatcg atggatggct tgatatcgtg
      961 agacacccca tgataggtca tgtacaaata gttcgtctct gcatcaaact ccccagccag
     1021 ggtgtctatt tgttttgtca caggataaac gcccagcttt aagcgttcgg ttctaaaatc
     1081 ctgttcggaa agatttttaa agcgccccaa aactttatcg ctgaatccca gttgtttggc
     1141 ttttcgaacc tgcgattct
//
LOCUS       DATDUN010000078         1395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_128427, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000078 DATDUN010000000
VERSION     DATDUN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128427"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VJC18_01115"
     CDS             complement(<1..463)
                     /locus_tag="VJC18_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HLD44005.1"
                     /translation="MTEHNTIREEISLKSYREKVRRAATYRIRGKVKELTGLIVRAVV
                     PNVSVGDLCYINIQNQGKTIKSEVVGFQGSDVLLMPLGNLEGIGPGNEVIPTGDCLRV
                     PVGFNLKGRILDGLGDPIDTDTRGGLDVKEYYPVHNTPPDPLKRKVICQPLS"
     gene            complement(460..1152)
                     /locus_tag="VJC18_01120"
     CDS             complement(460..1152)
                     /locus_tag="VJC18_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HLD44006.1"
                     /translation="MEQKSKVIKRDCVDEQISLENRLELEEFAEKESPVSSKIISESE
                     VQALSQSEVIVNKAQEESKRVKEKAKELYLQVETKMAEAKKQGFEQGRQEGLATVTGQ
                     LVQIEAHQKKLIESIEKESVSLVYEIAQKIVGNALKTDDEAVLGLIKQALHASLGNHL
                     TIFVNPNDYERIREKETQLTNLLQTTQTLSIKPTENVKEAGCVIESEMGTIDAQLDYQ
                     LEAIKKALGVGS"
     gene            complement(1178..>1395)
                     /locus_tag="VJC18_01125"
     CDS             complement(1178..>1395)
                     /locus_tag="VJC18_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44007.1"
                     /translation="SDRFVKTLGFLVFAKMLLMKDRQDLEIIKRKMSRQDANLMQDFV
                     NEQMEKNSEVSVIAFREDMLSAVSSVI"
BASE COUNT          291 a          384 c          306 g          414 t
ORIGIN      
        1 cggacagtgg ttggcagatt accttgcgtt taaggggatc gggcggcgtg ttgtgtaccg
       61 gatagtactc tttgacatcc aatccccccc gcgtgtcggt atcgatgggg tctcccaagc
      121 catccagaat gcggcccttc aagttaaacc ctaccggtac gcgcagacaa tctccggtgg
      181 ggatgacttc gttacccgga ccgatcccct ccaaattacc caatggcatc agcaggacgt
      241 cactgccctg aaatcccact acttcggatt tgatggtttt gccttgattc tgaatgttga
      301 tgtaacacaa atccccaaca ctcacgttag ggaccaccgc ccgcacgatc aagccagtca
      361 attccttgac cttgcctctg atgcgatacg tcgccgcacg acgcactttt tcgcggtaac
      421 ttttaagact gatctcttcc ctgattgtgt tgtgctcggt cacgaaccca cccccaacgc
      481 ttttttgatg gcctcgagct ggtagtccag ttgtgcgtca atggtcccca tctcggactc
      541 aatcacacag cctgcttctt tgacattttc ggttggcttg atgctcagcg tctgcgtggt
      601 ctgcaaaaga ttcgtcagtt gcgtttcttt ttctctgatg cgctcgtaat cgttggggtt
      661 gacaaaaatg gtcaggtgat tgcccaaaga cgcgtgcagg gcttgtttga tgagtcccaa
      721 gacagcctca tcatcggtct tgagcgcgtt gcccacaatt ttttgcgcga tctcatacac
      781 cagactcacg gattcttttt cgatactttc gatgagtttt ttctggtgtg cttcaatctg
      841 tacgagttgc cctgtcacgg tagccaaccc ttcctgccgt ccctgttcga aaccctgttt
      901 cttggcctcg gccattttgg tttcgacctg cagatacaat tctttggcct tctccttgac
      961 gcgtttggat tcttcctgtg ccttgttgac gatgacctcg gattgactca gcgcctggac
     1021 ctcggattct gaaatgattt tactggaaac aggtgattct ttttcggcaa attcctccaa
     1081 ctcaagacgg ttttcaaggc tgatttgctc atccacacag tccctcttaa tgacctttga
     1141 tttttgctcc atagacattt tcctcagtca gctagtgcta gatcacgctc gaaacagccg
     1201 acagcatgtc ttcgcgaaag gcgatgactg atacctcaga atttttttcc atttgctcat
     1261 tcacaaaatc ctgcataagg ttcgcgtcct gccgagacat ttttcgtttg atgatttcca
     1321 aatcctgccg atctttcatg agcagcattt ttgcaaaaac cagaaacccc aacgttttaa
     1381 caaaacgatc ggagt
//
LOCUS       DATDUN010000079         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_337633, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000079 DATDUN010000000
VERSION     DATDUN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337633"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VJC18_01130"
     CDS             complement(<1..621)
                     /locus_tag="VJC18_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLD44008.1"
                     /translation="MSPLHHLTIASAHQKIKNKEISVTELVQAFLDRIHKIDPELKSY
                     LTVCDEQAIRQARRIDADLAKGKPILPLTGIPLAPKDIYLTKGIKTTCASRMLQNFVA
                     PYNATVVQNCLDAGAVIVGKTNMDEFAMGSSTENSFFKTTRNPWDQTRTPGGSSGGSA
                     AAVSASLCLASLGTDTGGSIRQPASFCSIVGLKPTYGRVSRYGTIAF"
     gene            complement(844..1125)
                     /gene="gatC"
                     /locus_tag="VJC18_01135"
     CDS             complement(844..1125)
                     /gene="gatC"
                     /locus_tag="VJC18_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLD44009.1"
                     /translation="MISKTDLKKIASLAKIEITENELPALSQQVGQILEYVQTIDELN
                     LVGIVPTSHAVFVNNVFRDDVVINNHISEDVLSQAPESDGEFFKVPRVL"
     gene            complement(1122..1319)
                     /locus_tag="VJC18_01140"
     CDS             complement(1122..1319)
                     /locus_tag="VJC18_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44010.1"
                     /translation="MVNFRGSWIVGRESWIVNRISGSMDPSISAIGLIPFAGRWDDNR
                     YIHDPRSTIHDPRSTIHGILP"
     gene            1458..2276
                     /locus_tag="VJC18_01145"
     CDS             1458..2276
                     /locus_tag="VJC18_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HLD44011.1"
                     /translation="MTQVQITLIVEAIIFGLVVGSFLNVCIYRLPKNMSILGRSLCPR
                     CKKTIPLYRNIPLISYLLQRGKSACCKEVISSQYPIVEALTALLSLVTLLHSHTFVEY
                     IVWFCLFICPLIIVSVVDFQLKIIPDQISIPFIVVGIAVRIYASYPHWTDALITSGIG
                     ILAGGGSLLLLGEIISRLKKRDAMGGGDIKLAAMLGAFLGWRAIVFIFFASSILALIY
                     ALFCVVARKQAEDKTIPFGPFLSMGAMIAFWYGKTITDWYFLNVANLPFNVLFP"
BASE COUNT          568 a          576 c          602 g          720 t
ORIGIN      
        1 aaacgcgatg gtgccataac ggctcacccg gccataggtt ggtttgagcc ccacgatgga
       61 acaaaacgag gctggttgcc ggatagagcc tccggtgtcg gttcccagcg aggccagaca
      121 cagagaggca ctcacggcgg cggcagaacc acccgatgac ccacccgggg tacgtgtttg
      181 gtcccaggga tttctggtcg ttttaaaaaa agaattttct gtggacgagc ccatggcgaa
      241 ctcatccata tttgttttgc cgacgatcac ggcccccgcg tccagacagt tttgcacgac
      301 cgtggcattg taaggcgcga cgaagttttg gagcattctg gaggcgcacg tggttttgat
      361 gcctttggtc agataaatgt ctttgggggc cagggggatg ccggtgaggg gcaggattgg
      421 tttgcctttg gcaaggtcgg catcgattcg ccgggcctgt ctgatcgcct gttcatcaca
      481 gacagtaagg tacgacttaa gctccggatc aattttgtgt attctgtcga gaaaggcctg
      541 taccagttcg gtgacactga tctccttgtt ttttattttt tgatgggcgg aggcgatggt
      601 gagatgatgt aatggagaca tagtttatgt gtttgcgtgt ccaggtgttc acgtgttccc
      661 tagggggcat aaggcataag gcccaaggcg taaggcaaaa aactgcttca attgtctcaa
      721 tactcaattg acaattgatt gttgaagcag ttgaaaattg taacaattgt attcttccct
      781 ttcacctttt accgccagag gacaaatcgt ccatcgtcct ttcgtccctc gtcccttcgt
      841 tccctataag accctcggta ccttaaaaaa ttccccgtca ctctccggtg cttgtgagag
      901 cacgtcctca gaaatatgat tgttgatcac gacatcatcc cgaaacacgt tgttgacaaa
      961 aacggcatgg gaggtgggga caatgcccac cagattcagt tcatcaatgg tttgcacgta
     1021 ttccaatatt tgcccaacct gctgtgacaa ggcgggcaat tcattttccg taatttcgat
     1081 ttttgccaga gaggcgattt ttttgaggtc ggtttttgaa atcatggcaa aatcccgtgg
     1141 atcgtggatc gtggatcgtg gatcgtggat cgtggatcgt ggatataacg attgtcatcc
     1201 cagcgtcccg caaacgggat cagtcctatg gctgaaatgc tgggatccat gcttcctgaa
     1261 attcgattca cgatccacga ttcacgaccc acgatccacg atccacgaaa atttaccatt
     1321 gtcatctcat ttgaagcaag ttatggttgt atttatttat attagaaagc ctttgttgtt
     1381 caaccataag tatttctaac ttttcatgag ctctattgat ctcatgaaaa gttgaaatgc
     1441 tatagaagtt ttttaccatg acacaagtac aaatcacatt gattgttgag gccattatat
     1501 ttggtcttgt agtggggtct tttttgaatg tgtgcattta ccgcctgccc aagaacatgt
     1561 cgattctggg gcgcagtctg tgtccgcgat gcaaaaaaac catccccctt tatcgcaata
     1621 tcccactcat ttcttatctg ttgcaaaggg gtaaatcggc ctgttgcaaa gaggtcatca
     1681 gttcacaata ccccattgtt gaggccctca cggcgttgtt gtcgctcgtg acccttctgc
     1741 actcacacac gtttgttgag tacatcgtgt ggttttgtct ttttatctgt ccgctcatca
     1801 ttgtgtcggt ggtggatttt cagctcaaga tcatccccga tcagatttcg atccctttca
     1861 ttgttgtggg gatcgctgtg cgcatatacg cctcttaccc gcactggacg gacgccctca
     1921 tcacgtcggg gatcggcatt ctggccggag gtgggtcctt gctgcttttg ggggagatca
     1981 tctcgagact caaaaaacgc gatgccatgg gcgggggcga catcaaactg gcggcaatgc
     2041 ttggcgcttt tttgggttgg cgcgccattg tttttatttt ctttgccagt tccatcctgg
     2101 cccttatcta cgcgcttttc tgtgtcgtgg cgcgcaaaca agccgaagac aaaaccattc
     2161 cctttggccc ttttttgtcg atgggtgcca tgatcgcctt ctggtacggc aaaacgatca
     2221 ctgattggta ttttttgaac gtagcgaatc tgccgtttaa tgttttgttt ccttaagcgt
     2281 gccttctaaa aaacattgat aacagttatc attatatgtt atcatatacg tcatgagaac
     2341 catcgttgac atcccgaaca attgcctcgt gttacttaag gaattgtgtg cgcgtttgaa
     2401 tgaatcgaga tcacaaatca tcaggcaagc catcgaagaa tatctaaaaa aactggtcac
     2461 cacagg
//
LOCUS       DATDUN010000080         8955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_169684, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000080 DATDUN010000000
VERSION     DATDUN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169684"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VJC18_01150"
     CDS             <1..621
                     /locus_tag="VJC18_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44012.1"
                     /translation="IDMKNTFPIIIATIVFAVFLFSFVSEAQAGKTNLYKDKREYITV
                     KAKKGENFNHPRTLTPQQLTDLLSKVQYRHCQFICVDSSAPEPAFTHRQIALLSQHAA
                     KGLEQASNTELVEFFLTTPSRDFKRYTKGKLFIINDELHIIFDRLSEAGADNDPGQYQ
                     HGQGRWQVIASGNQRVQINPYGSSQKNWLVLSLASETSKKGSVKKK"
     gene            complement(672..1847)
                     /locus_tag="VJC18_01155"
     CDS             complement(672..1847)
                     /locus_tag="VJC18_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44013.1"
                     /translation="MKQKWCHLVFLLLVLMIVGCSSSEAPEEYGFEDDASVGTIITNP
                     KPPTKVVADNIFPDFDAQLPGLMEKEKFPLNQVSLPLENNVAVRTMLIKIFNMGSIRE
                     FNSELLLQELRSFGYAVQNQMNAEGVILPPTGELQVTISKKLGLLGYVAKAWQIRILP
                     EQNHDGYTRLYFINPDKGDIQASYVYKTSSDGQPIKGMLGFASPKALDEDLKETEAIR
                     LLVIAFDFSSPDRNVMTIRVDRLHDVLGSLAGQSFIQCHLGSSVCVAEHQEIFGEAPL
                     RVLAKPIVRYSWEDKLDDVCFASMDYSENHVQMSAPLYFEINSLAVTKQACDIPTPIW
                     GAHSFSVDDLLMRADDTDPLGGTARLVFTDGQSTEGWETYVDPALIDVTLDGSGLMN"
     gene            complement(1844..2845)
                     /locus_tag="VJC18_01160"
     CDS             complement(1844..2845)
                     /locus_tag="VJC18_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD44014.1"
                     /translation="MGFNHDLSTNVAEKAITVDAVLKNIDKLNSCKKTCPLIHEVSLA
                     SLFEHDIMECSMTVFDFTDYREFLRAYYEEQKKKYPFFSYRYFARLADIGGQSYLQMI
                     MSGKRNLSAKTVQKFIKAIKLKKKDAQYFETLVLFNQTKKEDERELYLDRLLCLKPAK
                     KISQLQQDRYLYFTNKNLVILREMVSLPHFEEDPEWISRSLNGAITPKEVVRIIELLI
                     KLDLIKRNDEGKLVLSSGSVKTPQDVESLEAYQYYRSIFNDAKEAILTVPFDKREVDS
                     LTIPISDKHIPKIKEIIQTSLEEIVKLVNSEKEPFDDVYQINTIFYPVTKTGKKEGV"
     gene            complement(3063..4517)
                     /locus_tag="VJC18_01165"
     CDS             complement(3063..4517)
                     /locus_tag="VJC18_01165"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HLD44015.1"
                     /translation="MTNQSFSECLLSTENHQAFITTAQDVRRSIGVPEVLRDPIRNPE
                     LENWFVSEAKQRLASLPANAVGQQRQDLLESPNRYPDGSMRPISLAELNQMTRLCGSA
                     GDILNRASQGRELLTNNDRHPLYGTAARGVPGGGASSRAAKWTRMNPDVSRRFGLAET
                     TPRPLFAVGSKTLLQHTIDFSQHIARAVGRIFPNIVMTSSENAGLVLDTIKRDWGNHT
                     PEELNSTVLFNQIVLPRLWVDDQTLTTDKLYPAGHGDYAYLFALYHLAQTLHEVGIRY
                     LVYSNADEWLWQADPVMISIAQEFFDQGHHMLIIGTKNTNNQFGGGFVRFADGRQSLV
                     ETPRLPWEIVQTGQAPIALNTTFYIIDVAYLAEHEAELLAVEKSLVVKEVPGRKPGAV
                     EQIIGVDSWAGDVFAACLNPAFVEWPRLNFLGIKDGGFVTGREHRPELGGRSYLHYLS
                     ETIATYPVIMKALLDGDRDVAQFLFETGYSYLAP"
     gene            complement(4606..4971)
                     /locus_tag="VJC18_01170"
     CDS             complement(4606..4971)
                     /locus_tag="VJC18_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD44016.1"
                     /translation="MQKKKVLIVDDEIELSEVILYRLEALGYDVQLAENGRVGVDKAI
                     QYSPDLILLDALMPVMNGYEASLQLKEDKRTQNIPVILFTAALDTSIGRKCLKYSDDL
                     LVKPYDTQELVQKIEQLVN"
     gene            complement(5266..6093)
                     /locus_tag="VJC18_01175"
     CDS             complement(5266..6093)
                     /locus_tag="VJC18_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HLD44017.1"
                     /translation="MAQKYPNTQMGHSNQQYSFSSYHIVWQFKLKELDKVIDAKPITL
                     TPLVLGYQFDQHSFCFVYNFGSVVFFNVGTAKQTQIINGIQTHLNKIFDLSVMDEYFL
                     ELNPGEKNRVAFNRVIVDSIDKDKIEMISLILAQSTALDFFEHKVDSLLTDVQDLTLI
                     VGKKARQISEKAILKLIKRIIAIKQDIIVSLRLLEKPEAAWEKKVLDVLYEEGIAMFE
                     LKERYSFLKEKLQMIQSNLEVLNSFSTNRKMLVLETAIVGLFILDVFLVLVELFIKA"
     gene            6289..7293
                     /locus_tag="VJC18_01180"
     CDS             6289..7293
                     /locus_tag="VJC18_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44018.1"
                     /translation="MHKSYRAQLIVALLILLFSSFPGTILAQVTPADSSTADIEITDP
                     FRHIVFLVPSNDWDVRVEDLMGTSLSRTDEATLSSIRVVDSYLEGNTSLEDVWQGKLS
                     YLKKNKPGYVYYKERDDLMIDNTKAIGITYKDPADQSIVRLIFFKTQNKYRELTFDIG
                     SEAIFQKIKPQLAFILKNIRIPSSPISNLAISDPYLGITMSVPGNKEWGFETASPYKR
                     FYYIPAGQYSTRGKINLFHSIYSVNSAQSANEEWNALVLKNLPGVEFLIKGQSFAHKG
                     IEMYSSTFKNPVAKTVVRGLNFIHRNSSLMIEFIIEEDFFDQVKSDFSTIMKNIVIVP
                     "
     gene            7296..8183
                     /locus_tag="VJC18_01185"
     CDS             7296..8183
                     /locus_tag="VJC18_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLD44019.1"
                     /translation="MPPKKTKQPPRNTRKSTRVLLSPATPNTIDALNQRLREALNKLQ
                     ESEQKLLNTNIELQRLDRLKDDFISTVSHELRTPLSIIIEVASSLHDNILGPLNEKQA
                     RIVDILFGNANRLGHLINNLLDISQLESERIKPRRMPLNVRELIEVTYHSFSKIANKQ
                     GIELEMKIGKKLPELRADSDMLTQVLHNLVSNALRFANTRITIEVKQNSDNMQFTITD
                     DGKGIETEELHKLFNKFEQIARPVGGDGYKGTGLGLAICKKIVELHDGQITCHCEPNK
                     GCTFSFQLPIKNHQPSQTR"
     gene            8309..>8955
                     /locus_tag="VJC18_01190"
     CDS             8309..>8955
                     /locus_tag="VJC18_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLD44020.1"
                     /translation="MNQKDYLALEDLYGAHNYKPLNVILSKGKGIWVWDIDGNKYMDC
                     LSAYSAVNQGHCHPKIMQAMIEQANKLTLTSRAFRNDQLGLLYQELCALTKSQKALPM
                     NSGAEAVETAIKAVRKWGYLVKSVPQDKAEIIVCADNFHGRTTTIVGFSTDPVARNDY
                     GPFTPGFKIVPFGDFAALEKAITPNTVAFMVEPIQGEAGVLIPPAGYLKQVRQLCT"
BASE COUNT         2408 a         2367 c         1927 g         2253 t
ORIGIN      
        1 attgatatga aaaatacatt ccccatcatc atcgcaacga ttgtctttgc ggtttttttg
       61 ttttcctttg tcagcgaggc acaagccggc aaaacgaatc tctacaagga taagcgcgag
      121 tacatcacgg tcaaagcaaa gaaaggtgag aactttaatc acccacgcac gctcactccc
      181 caacaactca ctgaccttct ctcgaaggtg caataccggc attgccaatt catctgtgtc
      241 gacagctctg ctcccgaacc cgcgttcacg caccggcaaa tcgccctgct ctcacaacac
      301 gccgccaagg gcctggaaca ggccagcaac accgaactgg tggaattttt tttgacgaca
      361 ccttcccgtg atttcaaacg ctacaccaaa ggcaaactct tcatcatcaa tgatgaactg
      421 cacatcattt tcgacaggct ctccgaggca ggtgccgata acgatcccgg ccagtaccag
      481 catggtcaag gcaggtggca ggtgatcgct tcagggaacc aacgcgtgca aatcaacccc
      541 tacggttcaa gtcaaaaaaa ctggctggtt ttgagtttgg cctcagagac ttcaaaaaag
      601 ggatctgtaa aaaagaagta ggcgccagtc attgcgagtt ggccaaaggc cagcgtggca
      661 atctccctga ttcaattcat caacccgctt ccatccaacg tcacatcgat cagagcagga
      721 tccacgtacg tctcccagcc ctcggttgac tgtccgtctg taaaaaccaa tctggcagtt
      781 cctcccagcg ggtcggtgtc atcggccctc atcaacagat catccacaga aaatgagtgt
      841 gccccccaaa tgggtgtggg aatgtcacaa gcctgcttgg tcacggcaag cgaattgatc
      901 tcaaaataca acggcgcgga catttgtacg tgattctctg aatagtccat gctggcaaaa
      961 cacacatcat ccaatttatc ctcccaactg tagcgcacaa tcggtttggc cagaacacgc
     1021 agtggggcct caccaaagat ctcctgatgt tccgccacac acactgatga gcccaaatgg
     1081 cactgaataa acgattgtcc agccaaactg cctaacacat cgtgcaaacg atccacccga
     1141 atcgtcatga cattcctgtc ggggctgctg aaatcaaagg caatgaccaa aagtctgatg
     1201 gcctcagtct ctttcagatc ttcatccaac gccttgggac tagcaaaccc cagcatcccc
     1261 ttgatgggtt gcccgtcaga agatgtcttg taaacataac tcgcctgaat gtcaccttta
     1321 tcgggattga taaaatacag gcgcgtgtac ccgtcatgat tctgttcagg cagaatgcga
     1381 atctgccaag ccttggccac ataccccaga agacccagtt ttttggaaat cgtgacttgc
     1441 aattcgcctg ttggtggcaa gatcacacct tccgcattca tctgattttg cacagcgtaa
     1501 ccgaacgacc tcaattcctg caataaaagc tctgaattga attccctgat actgcccatg
     1561 ttgaatattt tgatcaacat cgtcctcaca gccacattgt tttcaagagg caggctcacc
     1621 tgattgaggg gaaacttctc tttctccatc aaaccgggaa gttgggcatc aaaatcagga
     1681 aagatattat ccgccacgac ttttgtcggt ggcttgggat tggtgatgat cgtccccacg
     1741 gacgcgtcgt cctcaaatcc atattcctct ggggcttccg aagaagagca accgacaatc
     1801 atcagaacca acaacaggaa aacaagatgg caccattttt gtttcataca ccctctttct
     1861 tacctgtttt ggtcacggga taaaaaatgg tgttgatctg gtacacgtcg tcgaagggct
     1921 ctttttcgct gttcaccagt ttgacgatct cctcaagact ggtttggatg atctccttga
     1981 ttttgggaat gtgcttgtca ctgatgggga tcgtgaggga atccacctca cgtttgtcaa
     2041 acggaacggt gaggatcgcc tctttggcat cgttgaaaat ggaacgatag tattgatagg
     2101 cctccagcga ttcaacatcc tgcggggttt tcacgctccc gctcgacaac accagcttgc
     2161 cctcatcgtt gcgtttgatc aaatcgagct tgatgagcaa ttcaattatt ctcacgacct
     2221 ctttgggcgt gatggcccca ttcagcgatc ttgaaatcca ttcaggatct tcctcaaaat
     2281 gagggagaga caccatctcg cgaaggatca ccagattttt atttgtaaag tataaataac
     2341 gatcttgctg caactgagat atttttttgg ctggcttcaa gcacaacaac ctgtccagat
     2401 agagttctct ttcatcttct ttttttgtct ggttgaacag aaccagagtt tcaaaatact
     2461 gggcatcttt tttcttgagt ttgatggcct tgatgaattt ctgcacagtc ttggcgctca
     2521 aattgcgctt gccgctcatg atcatctgca aataactctg tccgccaatg tccgccagtc
     2581 gcgcaaagta tcgataagaa aaaaacgggt attttttctt ttgttcttcg taataggccc
     2641 tcaaaaattc gcgataatct gtaaagtcaa aaactgtcat gctacattcc attatatcat
     2701 gttcaaagag ggatgccagt gaaacttcgt gaatgagtgg acatgtcttt ttgcaactgt
     2761 ttaatttatc gatgtttttt aaaacagcgt cgacggtaat tgctttttct gcaacatttg
     2821 tggacaggtc gtgattgaaa cccatactcg ctcccttcgt gtgatcatgc atacggcgta
     2881 cagtacatat attggttatc ggctgacttg tccaaaggtt gcgcctctac gcataaaacc
     2941 gtccacaatt gtggacacct gccaaaggcg aattcatctg atgaatgtga ttattcctgt
     3001 gaatacagcg gaatattttt ggcgtggcac gcaaagaaaa ccgcaattgc ctttgtggtc
     3061 agtcacggtg ccagataaga gtacccggtt tcaaacaaaa attgggccac atcacggtca
     3121 ccatcgagca gggccttcat gatcacggga taagtggcta ttgtctcact cagataatgc
     3181 agataggagc gaccacccaa ctcgggtctg tgttcacggc ctgtcacaaa accgccatcc
     3241 ttgatgccca aaaaattcag gcgcggccat tccacaaagg ccggattaag acacgcggca
     3301 aagacatcac ccgcccaact gtccacgcca atgatctgct ccacagcacc tggtttgcgt
     3361 cccggcacct cttttacgac caaagatttc tcgacggcca gaagttcggc ctcatgctcg
     3421 gccagataag ccacatcgat gatgtaaaac gtggtgttga gggcaatcgg tgcctgcccc
     3481 gtctgaacaa tctcccacgg gagtctgggg gtctccacca atgactgtct gccgtcagca
     3541 aagcgcacaa acccaccgcc aaattgattg ttggtgttct tggtaccgat gatgagcatg
     3601 tgatgccctt gatcaaaaaa ttcctgcgca atggaaatca tcacgggatc ggcctgccac
     3661 aaccactcat cggcattgga atagaccaga taacgaatac ccacttcgtg caaggtctgc
     3721 gccaggtgat acagagcgaa caagtaggcg tagtcaccat gccccgcggg atagagctta
     3781 tcggtcgtga gggtctgatc atcgacccac aggcgcggca ggacaatctg attgaacaaa
     3841 acggtgctgt tgagttcctc tggcgtgtga ttgccccaat cccttttgat cgtgtccaac
     3901 accaggcccg cattctcgga gctcgtcatg acaatgttgg gaaatattct tcccacagca
     3961 cgcgcaatgt gttgcgaaaa atcgatcgtg tgttgcaata atgttttgga acccacggca
     4021 aaaagcggtc gtggtgttgt ttcggccaat ccaaatcgcc tggacacatc gggattcatg
     4081 cgagtccact tggccgcgcg cgatgaggcc ccacctcccg gaacgccgcg cgcggccgtg
     4141 ccatagagcg gatgtcgatc attattagtc aataactcac gaccctggct ggcacgattg
     4201 agaatatcgc ccgccgaccc acacaatcgt gtcatttgat taagttctgc gagcgaaata
     4261 ggacgcatgc tcccgtcagg ataacggttg ggagattcca gcaggtcctg cctttgctgg
     4321 cccactgcat ttgcgggaag tgatgccaat ctttgtttgg cctcactgac aaaccagttc
     4381 tcaagttcgg gattcctgat ggggtccctc agaacctcag gcactccgat gctccgccga
     4441 acgtcttgag ctgtggtgat aaaggcttga tgattttctg tagataacaa acactccgaa
     4501 aaactttgat tggtcatgtg acacgctcct cgtccgccct ataaagccgg accttgcgcg
     4561 tcaaatgtat tttgacaaat tcaacccatg acacatgacc agatattagt tcaccaactg
     4621 ttcgattttc tgtaccagtt cctgcgtgtc ataaggtttg accagcaaat cgtctgaata
     4681 cttcaaacac tttcgaccaa tcgatgtgtc aagtgcggca gtgaacagga tgacaggaat
     4741 attttgtgtc cgcttgtctt ctttcaattg caacgaagct tcatacccgt tcatgaccgg
     4801 catgagcgca tccagaagta tgagatcggg actatattga atcgccttat cgacaccgac
     4861 ccgtccattt tccgccaact gcacatcata ccccaaggct tcgagcctgt acaatatgac
     4921 ctcggacagt tcaatttcgt catccacgat caggactttt ttcttttgca taacgatctc
     4981 ccgttttggg ttctgggtca tgggtactgg taaatgccat ccagaaccaa taacctacca
     5041 tctacccatg caagaacaat accaaaccac attattctat ttgattgatt ttacagtatt
     5101 ttttttatgg acagctctca atatgagaac catttggtaa gattatcaat tcgatattcg
     5161 atactagata ttcattgccc tttagcctta cacctctcgt gaacacatga acacatgaac
     5221 acgcctgccc cctgcggggg tgaacacttg aacacgtttt tcgccctacg ccttgataaa
     5281 cagttccacc agaaccaaga acacatcgag aataaaaagg cccacgatgg ctgtttcgag
     5341 gacaagcatt ttacgattcg tggaaaaact gttcaacact tcgaggttac tctgaatcat
     5401 ttgaagtttt tccttaagaa agctgtaccg ctccttgagc tcaaacatgg caatgccttc
     5461 ttcgtacaac acatccagca cttttttttc ccaagcggcc tccggttttt cgaggagtcg
     5521 caaagacaca atgatgtctt gtttgatggc aatgatccgt ttgatgagct taagtatggc
     5581 cttttcgctg atctgccgcg ccttcttgcc aacaatcaac gtgaggtcct gcacatcggt
     5641 caataaggaa tcaaccttat gttcaaaaaa atccagggct gttgattgcg ccaaaatgag
     5701 cgagatcatc tcgattttat ctttgtcgat gctgtcaaca atcacccgat tgaaagccac
     5761 cctgttcttc tcgcctggat tgagttccaa aaaatactca tccatcacag aaagatcgaa
     5821 aatcttgttc aggtgggttt gaatcccgtt gatgatctgt gtttgtttgg ctgtcccgac
     5881 attaaaaaaa acaacagaac cgaaattgta gacaaaacag aacgagtgct gatcaaattg
     5941 atatcccaac accagaggtg tcagggtaat gggtttcgcg tcgatcactt tatcgagttc
     6001 cttcaatttg aactgccaaa cgatgtgata cgacgaaaag gagtactgct gatttgagtg
     6061 ccccatctgc gtgttagggt atttttgagc catggttctt cctagtgcct cgcccgcatg
     6121 aaagttagac tttcaaggct ggctttcatg cggttgcgga cacttgtgct gagcatggaa
     6181 agtacactat ccatacggtc gaagtactaa aacgaatcgg aaggagtgac aacacgaaaa
     6241 aacacataaa aaaattgcca ttttctgata ttcatttagt atggcgccat gcataaaagt
     6301 tatcgtgcgc aactcattgt tgccctcctc attctcctgt tcagttcttt tccgggaacc
     6361 attttggctc aggtcacacc cgcagattct tccactgccg acatcgaaat aacagatccg
     6421 ttccgacaca tcgttttttt ggttccctca aacgattggg acgtgcgtgt tgaggacctt
     6481 atggggacta gcctgtcacg cactgatgag gcaaccctct cttcgatccg tgtcgtcgac
     6541 tcctatctgg aggggaacac ctctttggaa gatgtgtggc aaggcaaact cagttatctc
     6601 aaaaaaaaca aacccggcta cgtgtactac aaggagcgcg atgacctgat gatcgacaac
     6661 acaaaggcca tcggcatcac ctacaaagac cctgccgatc aaagcatcgt gagactcatt
     6721 ttcttcaaga cacaaaacaa ataccgtgaa ctgacgttcg acattgggag tgaggcgatc
     6781 tttcaaaaaa tcaagccaca actcgccttc atcctcaaaa acattcgcat cccctcctcc
     6841 cccatctcga acctcgccat ttccgatcct tatctgggga tcaccatgtc agtgcctggt
     6901 aacaaagagt gggggttcga aaccgcctcg ccatacaaac gattttatta catccctgct
     6961 ggtcagtatt ctacccgtgg caagatcaac ctgttccatt ccatttattc ggtaaattcg
     7021 gcccagtccg caaatgaaga atggaacgcg cttgtgctta aaaatctgcc cggagttgaa
     7081 tttttgatca aaggacagtc ttttgcgcac aagggtatcg agatgtattc gtccaccttc
     7141 aaaaatccgg ttgccaaaac agttgtgcga ggactcaatt tcattcatcg taacagttcc
     7201 ctcatgatcg agttcatcat cgaagaggat tttttcgatc aggtcaaatc cgatttttca
     7261 acaatcatga agaacatcgt catcgtaccc tgaatatgcc cccaaaaaaa acaaaacaac
     7321 ctccccgtaa tactcgcaaa tcaacacgag tgttgctttc tccagcaacc ccgaacacaa
     7381 tcgacgcgct caaccagagg ctcagagaag ccctcaacaa actgcaggaa agcgagcaga
     7441 aattattgaa caccaacatc gaactccaaa gactcgacag gctcaaagac gacttcatct
     7501 ccaccgtctc acacgaatta aggacaccgc tttccatcat catcgaggtc gccagctcac
     7561 tgcacgacaa tattctgggc cccctcaacg aaaaacaggc gcgcatcgtc gacattcttt
     7621 ttggcaatgc caatcggctg ggacacctta tcaacaatct tctggacatt tcccagctgg
     7681 aatccgaaag gatcaaaccc cgtcgcatgc ccctgaacgt gcgagagctc atcgaggtca
     7741 cctaccactc tttctcaaag atcgccaaca aacaaggcat tgagttagaa atgaaaattg
     7801 gcaaaaaact gccagaactc cgcgccgatt ccgacatgct cacacaggta ttgcacaatc
     7861 tggtcagcaa tgcccttcgc tttgccaata ccaggatcac tatcgaggtt aagcagaaca
     7921 gtgacaacat gcaatttacc atcaccgatg acggtaaggg aatcgagacc gaggaacttc
     7981 acaaattgtt caacaagttc gaacaaattg caagacctgt tggtggcgat gggtacaagg
     8041 gcactggttt ggggctggcc atttgcaaga aaatcgttga gttgcacgat ggccagatca
     8101 cctgccattg cgagcccaac aagggatgta ccttctcatt tcaacttcct atcaagaatc
     8161 accaaccttc ccaaactcgc taatttcatt attaatttta cgactcagcg atcacaagtt
     8221 aggactttac gggaatttta agacgtgata acccctcttc ggccaagttc caaataaccg
     8281 ttgtccttgt tttaccggag attttattat gaaccaaaaa gactatctcg cactcgaaga
     8341 cctttacggt gcccacaatt acaaacccct caatgtcatc ctttcaaaag gcaagggcat
     8401 ctgggtctgg gacatcgacg gcaacaaata catggattgt ctgtctgcct attccgcggt
     8461 caatcagggt cattgccatc ccaaaatcat gcaggccatg atcgagcagg caaacaagct
     8521 cacgctcacc tcacgtgcct tccgcaacga tcaacttggt ctgttgtacc aggaactgtg
     8581 tgctctcaca aaatcgcaaa aggccctgcc catgaacagc ggcgctgagg ccgttgagac
     8641 cgccatcaag gcggtcagaa aatggggtta tctggtcaaa agtgtccctc aagacaaagc
     8701 cgaaatcatc gtgtgcgccg acaactttca cggccgcacc acaacgattg tgggctttag
     8761 tactgacccc gtagcccgca atgattacgg ccctttcaca ccgggtttca agatcgtgcc
     8821 gtttggcgat tttgccgcac tggagaaggc catcacacca aataccgtgg cctttatggt
     8881 cgaacccatt cagggcgagg cgggagtgct catcccaccg gcaggttacc tcaaacaagt
     8941 cagacagctt tgcac
//
LOCUS       DATDUN010000081         1495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_294771, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000081 DATDUN010000000
VERSION     DATDUN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294771"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /gene="cbiQ"
                     /locus_tag="VJC18_01195"
     CDS             <1..566
                     /gene="cbiQ"
                     /locus_tag="VJC18_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HLD44021.1"
                     /translation="KTHPLLKLLLCLLCVLTTALLPRFFWQGFAVVATSLTLLLLLAR
                     VNGIFFLKRLLLFVPFALGLAGLTLCQPGGRRVFLDLFIRSNLCVWAVILLGVTTRFS
                     DILASLRRLHVPGLFITTLTLTYRYLFVLVDELQRFKRARQCRTFVPQGLLSWKALGN
                     IIALLFVRTADRADRVFNAMCARGWKT"
     gene            563..1303
                     /locus_tag="VJC18_01200"
     CDS             563..1303
                     /locus_tag="VJC18_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLD44022.1"
                     /translation="MKALEINDLSYHYAQGCDVLKHITLSVSPGEKVGVIGPNGAGKS
                     TLFLHLNGILPEKKITTPRIHVFGTPVVTAELPTIRTNVGLLFQDPDDQLFCPTVWED
                     VAFGPSQLGLTAEEVNTRVSEALMNAGLTDFEQREPHRLSVGEKRRVCLAGILACHSK
                     LLVLDEPTSNLDPRARRELTHLLKGLKDTQIIASHDLEFIVHTCSRVIVLDQGELVAE
                     GPTTAILSDEKLMLAHGLETPYILKCRK"
BASE COUNT          328 a          404 c          369 g          394 t
ORIGIN      
        1 tcaaaacaca tcccttgctg aagttgttgc tctgcctgct ctgtgtcctc acgacagctc
       61 tgctccccag atttttctgg cagggttttg ccgtggtggc gacaagtttg acgttgttgt
      121 tgctcctggc gcgcgtcaac gggatttttt ttctaaaacg gctgttgttg tttgttccct
      181 ttgcgcttgg cctggcggga ctaactctgt gtcaaccagg cggcagacgc gtttttttgg
      241 atttgttcat cagaagcaat ctctgtgttt gggctgttat cctgcttggt gtcacaaccc
      301 gtttttctga tatcctggcg tctttgcgcc gcctgcatgt ccccgggttg ttcatcacaa
      361 ccctgacact gacctatcgg tatctctttg tgctggtgga tgagttgcaa cgattcaagc
      421 gcgccagaca atgccgtacc tttgtccccc agggtttgtt gtcctggaag gccttgggaa
      481 acattattgc gctcctgttt gtgagaacag ctgatcgcgc cgatcgtgtc ttcaatgcca
      541 tgtgtgctcg ggggtggaaa acatgaaggc cctcgaaatc aatgatctca gctatcacta
      601 cgcacagggc tgtgacgtgc tcaagcacat caccctttcg gtctccccgg gagaaaaagt
      661 gggtgtgatc ggccccaatg gagcgggcaa atcaacactg tttttgcatc tcaacggcat
      721 tttgccagaa aaaaaaatca cgacaccccg catccacgtc tttgggacac ctgtggtcac
      781 agcagaatta ccaacgatca gaaccaatgt tggtctgctg tttcaagatc ccgacgacca
      841 gttgttctgc ccaaccgtgt gggaggatgt ggccttcggt cccagtcagt tggggttaac
      901 cgcggaagag gtcaacacac gggtaagcga ggcgctcatg aacgctgggc ttacggattt
      961 tgagcaacga gaaccacacc gtttgagcgt gggcgaaaaa cgacgcgtgt gtctggctgg
     1021 cattctggcg tgtcattcga aactattggt gctggatgag cccaccagca atctcgatcc
     1081 gcgcgcacgc agagaactca cccacctcct caaagggctc aaagacacgc agatcattgc
     1141 gtctcatgat ttggagttca tcgtacacac ctgttcccgg gtcattgttt tggaccaagg
     1201 agaactcgtt gccgaaggcc caacaacggc gatcctttct gacgaaaaac tcatgctcgc
     1261 acatggtttg gaaacacctt atattttgaa atgcagaaaa tgaaaacata ttcgtttccc
     1321 cctttgacct ttaacctttg agctaatatc gtcgccccat gtcccgcttc ctcctcactt
     1381 tcgcatacaa tggtgcccac tattccggac tccaattcca accacgacag acgaccgtgc
     1441 aagagctcat cgaaaaggcc ctctcacaag ttctgtgtga agcggttaaa gtcat
//
LOCUS       DATDUN010000082         1340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_543107, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000082 DATDUN010000000
VERSION     DATDUN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543107"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..>1340)
                     /locus_tag="VJC18_01205"
     CDS             complement(195..>1340)
                     /locus_tag="VJC18_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HLD44023.1"
                     /translation="YLQGTALVNPHLRLRYKFLDQEEAMLERVTDQVPTIPERTSPHP
                     HTMKLGEFLAHSHLFGKITTRKWFKEGFSRVSDSVIQDMIGAGIPVGWFEKALSILNE
                     DQLKQMYAVLQNVKLMAPPTKSVMAIGEADLAKSIQRLGEIDFFAVVSRKPTICDFKP
                     VQVEVAIARLKDKSEEERDTPVQILRFANRVPLQFDKAACAMTKAVESVNWRPYGLAQ
                     PKKTLPQGPYIFAVSVVSPFIKFKNASKETVDASDELVEEIRRTLQQCGQKLSAHINR
                     EAKAADVERKMQHIEQFAPILIDGLCRITFAKAGRKEKAIEGLAKILGRDSKEAEQEL
                     ATAHQKSRDAVREMADKLDLDHDELHDMMHGSDEEEAPMAGQLELLE"
BASE COUNT          315 a          387 c          305 g          333 t
ORIGIN      
        1 tgataattta ctgacatcga aatcagatct gtttctggtt gcagtctttg acataaaaaa
       61 tcgcaagtac aacagaatgg gcgtaaggcc aaaggcttaa ggcacaaggt caaagtccat
      121 gtgtttttat atccctaaac cttacgcctt ttgccctaag ccttgcgcct caagaacacc
      181 tgaacacgtg ctcatcactc caacaactcc aactgccctg ccatcggcgc ttcttcttcg
      241 tcgctaccgt gcatcatgtc gtgcaactcg tcgtgatcca aatcgagctt atcggccatt
      301 tctctcacag catcgcgtga cttctgatgg gccgttgcca actcctgttc agcctctttg
      361 gaatcacggc ccagaatttt ggccagaccc tcaatggcct tttccttgcg gcccgccttg
      421 gcaaacgtga tgcgacacaa accatcaatc agaatggggg cgaactgctc aatgtgctgc
      481 atcttgcgtt ccacatcggc cgccttggcc tcgcgattga tgtgcgcgga caatttctgc
      541 ccgcactgtt gcaaagtcct gcggatctct tcgaccagtt catcgctggc atcgaccgtc
      601 tccttggagg cgttcttgaa tttgatgaag gggctcacta cactcacggc aaaaatatag
      661 ggcccttgcg gcaaggtctt tttgggctgc gccaaaccat agggtcgcca attcacagac
      721 tccaccgcct tggtcatggc gcacgcggct ttgtcgaact gcaaaggcac gcggttggca
      781 aaacgcaaaa tctgcacggg agtgtcgcgt tcttcttcgg acttgtcttt taaacgcgca
      841 atggccactt caacctgcac cggtttgaaa tcacaaatgg tgggtttgcg agacaccaca
      901 gcaaaaaaat caatttctcc caaacgctga atgctcttgg ctaaatcagc ctcaccaatg
      961 gccatcacgc tcttcgtggg aggcgccatg agcttgacgt tctgcaagac cgcatacatc
     1021 tgtttgagtt ggtcttcgtt cagaatggag agggcctttt cgaaccatcc aaccggaatc
     1081 ccggcgccaa tcatatcttg gatcactgaa tcggacacgc gcgagaaccc ttccttaaac
     1141 cacttgcgag tcgttatttt tccaaaaaga tgggaatgcg ccaaaaattc gcccagcttc
     1201 atggtgtgcg ggtgtggcga cgtccgctcc ggaatggtcg gcacctgatc agtcaccctc
     1261 tccagcatcg cttcttcctg atccaaaaat ttgtaccgaa gcctcaaatg aggattcacc
     1321 aacgcagtcc cctgcaaata
//
LOCUS       DATDUN010000083         3177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_585681, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000083 DATDUN010000000
VERSION     DATDUN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585681"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VJC18_01210"
     CDS             complement(<1..537)
                     /locus_tag="VJC18_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44024.1"
                     /translation="MSEIIPFKKTVPPQDLVLRFMHSAGHKKDAEFYINLFSKTKAES
                     FAIIVLEEEGLRDEFDSILFEIRYLLRLSLYPVVLIRSTSDYMGDLELENYFKKAKLT
                     SNVLSHESTQDEKLEFVEQRIRRRELPVLHLDPQRDIILELTNLANLLRTSKIIFLKR
                     SGGIIDHETQTPIGLINLK"
     gene            879..1166
                     /locus_tag="VJC18_01215"
     CDS             879..1166
                     /locus_tag="VJC18_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44025.1"
                     /translation="MKTIKLMLLCGLLLVCNGHCYGGQDNAGKYSCVVDDDCTLSCAQ
                     GAVNTKWYKQFGGVKKDCLDGCAADGFDSKCENHICEAYLEGKKHPACTKK"
     gene            1487..1714
                     /locus_tag="VJC18_01220"
     CDS             1487..1714
                     /locus_tag="VJC18_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44026.1"
                     /translation="MRDIVRYSCARDTDCLLSCKEGAVNGRWYKQYTKHVTGFHECEG
                     GCESLGMVAKCENFICEAYLDGTKYPQCTRK"
     gene            1911..>3177
                     /locus_tag="VJC18_01225"
     CDS             1911..>3177
                     /locus_tag="VJC18_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44027.1"
                     /translation="MDTIKTIVRKRYYMVMTGLLCAVALFFLLSTAIQSQRLVGAQHP
                     GFLVFNNGLVFGLVDSSWNGNRAGLRMYDFIKGYDGHPFTGTKGLIDYINQKAPDGQV
                     QYLVTRNHQDITLAIKPMAIDQNFANLYFYIFLFCGFLTLLTGLGIFIIRPLAKGAFH
                     FFVICNTVSIFEILFADMMTYQTFLPYLQIIIALYGIVNIHTLYFMLRMREHLHVRLS
                     QLPVKIVLGGVYVALVAFALLVNLYSDRMFADYRVLLFASNFLFFIGGVLIYLTTAYL
                     YFSAKSSTAKRRLLLYMALNLSCFIGFAVYVAHNVCALLLPIELILLQLAFPFLTTYL
                     LFKYNFLDLKFTPRRKYFYLGFAVLLPMLFVVDFVFLIPILNKYQVAVSWVLPLVTAS
                     VVIKLMLLVGAAYLRIVDRLFFPSTFKFRD"
BASE COUNT          756 a          685 c          799 g          937 t
ORIGIN      
        1 cttgagattg atcagaccaa tgggggtctg cgtttcgtgg tcgatgatcc ccccactgcg
       61 tttgagaaaa atgattttac tggtgcgcaa aagattggcc agatttgtga gttccaggat
      121 gatgtcacgc tgtgggtcca gatgtaaaac aggcaattcc ctgcgacgga tgcgttgctc
      181 aacgaattcg agcttttcat cttgcgtgga ttcgtgcgac aagacgttcg aggtgagttt
      241 tgcttttttg aaatagtttt ccagttccag atcgcccata taatcgctgg tagacctgat
      301 cagcaccacg gggtacaaag acagtctgag gaggtaacgg atttcaaaga gaatcgaatc
      361 gaattcgtcc cgcaggccct cttcttccag cacgatgatg gcgaaactct cggccttggt
      421 ttttgagaaa agattgatgt aaaactcggc gtcttttttg tgacccgccg aatgcatgaa
      481 cctcaagacc aaatcctgcg gcgggactgt ttttttgaag gggataatct ctgacatgag
      541 cctcttaaaa cacgaattct gtattgttat caatgggaaa ctggtctttc gagtcagtgg
      601 tcaatgaagg gagcaaaacg ccacaagaca cgaaagggcg ttgaaccaaa atactaatga
      661 ttgttcttgc acccattttt cactattagg gacctctaaa aaccacccat ctgcggcgtt
      721 gcccactgca cccgcaatac tcacgtactt ccgtgtacgc tccgtttgcg ggtttgtggg
      781 cgccttgcat ctgggtactt tttagaggtc cccgaaaatg gtggttatta gagatgtctt
      841 attcttttgg tgatcgatga tacagagcgt ctcttggtat gaaaacaata aaactcatgt
      901 tgctttgtgg tttgcttctg gtttgtaacg gccattgtta tggcgggcaa gataatgccg
      961 gcaaatattc ctgtgttgtt gatgacgatt gtaccctgtc ttgtgctcag ggggcagtga
     1021 acacgaagtg gtataagcag tttggtggtg tcaagaagga ttgtctggat ggttgtgctg
     1081 cagatgggtt tgattcaaaa tgcgaaaatc atatctgtga ggcgtatctt gaaggaaaaa
     1141 aacaccctgc gtgcacgaag aaatgagcgc tcaagggaga tgaaatatgc gttatccaaa
     1201 aattgttttt gtattgttgt tcaccctcgt tggctttgcg tgtcataaat cagaaaacgt
     1261 cgaagcccca tattcctgtc aacaagattc aaattgcctc aactcctgcg ccttgggggc
     1321 agtgaataag tcctggtatg cgaaaaattc acagttagat acctgcgacg acggttgtac
     1381 gggttggagc gatgagccca ggtgtattga ccatttttgt acggcattcc aagctgggca
     1441 gtacaatgtc ttgtgtacga agggacctaa accaaaggaa aatgtcatga gagacattgt
     1501 tcgctattcc tgtgcgcggg acacggactg tttgttgtcg tgcaaagagg gggcggtgaa
     1561 cggccgttgg tacaagcaat atacaaagca tgtcacggga tttcacgagt gtgaaggcgg
     1621 gtgtgaatcg cttggcatgg tggcaaagtg cgaaaatttt atctgtgaag catatttgga
     1681 tggaacaaag tacccgcagt gtacgaggaa gtagataatt ctgaatgatt atgctgtttc
     1741 ccccgatcga gcgaacacgc attgaacacg taaaggtcac aaggcccccc ttgttccaag
     1801 cgacgagaaa tccactagcc aatcggctat gcactagcaa atccgctgta tttttatatt
     1861 gtcgtataat tccagttttg atagtccagt agggggagcg aaggaaagat atggatacta
     1921 taaaaacgat tgtcagaaaa cgctactaca tggtgatgac tgggttactc tgtgcagtgg
     1981 cgctattttt tttattgagc acggccattc aatctcaaag acttgtggga gcacagcacc
     2041 ccggatttct agtgtttaat aatgggctgg tgtttggctt ggtcgattca tcgtggaatg
     2101 gcaaccgcgc gggactgcgc atgtacgatt ttatcaaggg ttatgatgga catcccttca
     2161 cgggtacaaa aggacttatt gactacatca atcagaaggc cccagacggc caggttcaat
     2221 atctggtgac acgtaatcat caggatatca cattggccat caaacccatg gccatcgatc
     2281 agaattttgc caatctgtat ttttacattt ttctgttctg cgggttcctc accctgctca
     2341 cgggtctggg gatctttatc atcaggccat tggcgaaggg ggcgtttcat ttctttgtca
     2401 tttgcaacac agtcagcatc tttgaaatcc tttttgccga catgatgact tatcagacgt
     2461 tcttgccgta tctgcagatc atcatcgccc tgtatggaat agtcaatatc cacaccctct
     2521 acttcatgct caggatgcgg gaacacctgc acgtcaggct gagtcaatta cctgtcaaaa
     2581 tcgtgttggg tggcgtgtat gtggcgctgg tggcgtttgc attgcttgtg aatttgtatt
     2641 ctgatcggat gttcgctgac taccgtgtgt tgctgtttgc cagcaatttt ctgttcttca
     2701 ttgggggggt gctgatatac ctgacgaccg cttacctgta tttttcggcc aaatccagca
     2761 cggccaagcg gcggctgttg ttgtatatgg cactcaatct ttcctgtttc attggtttcg
     2821 ctgtctacgt tgcgcacaat gtctgcgcgt tgttgctccc cattgagctg attctcctgc
     2881 aattggcctt tccttttttg acgacatatc ttttattcaa atacaatttt ctcgatctca
     2941 agttcacacc tcggagaaaa tatttttatt tgggttttgc ggtgttgctc cccatgctgt
     3001 ttgtggtaga tttcgtgttt ttgattccca tcctcaacaa ataccaggtg gctgtgtcct
     3061 gggtcctgcc gctcgtgacc gcgtccgttg tgatcaagct gatgttgctg gtcggggcgg
     3121 cctatctgag gatcgtggat cgtctctttt ttccctccac attcaaattc agggaca
//
LOCUS       DATDUN010000084         1831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_585695, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000084 DATDUN010000000
VERSION     DATDUN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585695"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VJC18_01230"
     CDS             <1..854
                     /locus_tag="VJC18_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HLD44028.1"
                     /translation="ELGILYLLAVEALVVVGIVMSGWASNNKWSLLGGMRSAAQIVSY
                     EVPVSLTVLMVVLWCGSLNIKEIILAQGAWPWQWHVFKTPFLFVAFFVFFIGSLAEGN
                     RTPFDMAEAESELVSGYNTEYSGFRFILFFFAEFANLYLMGAISAILFLGGWNAGVVP
                     EFVIKTLGVETPWLAWLIKNLIEHIVFQTKSLGLVFIIIQLRWTLPRVRIDQLMGLCW
                     KYLVPIAFVNLVGMSLWMVFVTPKVTQWVAWGMTGLGGLIALHFIRRVAWHLRYTKAK
                     LRWNPFV"
     gene            1012..1458
                     /locus_tag="VJC18_01235"
     CDS             1012..1458
                     /locus_tag="VJC18_01235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HLD44029.1"
                     /translation="MSYLSNIVSSVKTVFEGMTITFSHLFREPMTIQYPDRTPRPVKE
                     TLPQRYRGFLATDMSICTGCTLCQKACPIDCIVIEATKDPEKGRVLTRFGIDLGKCMY
                     CGLCVEPCPTGAIAFTREFEGATPDLKTLQREFIRQGECVVPFKKA"
     gene            1559..>1831
                     /locus_tag="VJC18_01240"
     CDS             1559..>1831
                     /locus_tag="VJC18_01240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HLD44030.1"
                     /translation="MEQIIFILFFVITIGSACVVAFGRNLVYSAFALLGTFLGVAGIF
                     ILLHAEFLALVQVIVYVGGILVLTVFAVMLTSKIHASQTTNPALNLK"
BASE COUNT          376 a          385 c          550 g          520 t
ORIGIN      
        1 tggaattggg aattctgtac ttgttggcgg ttgaggccct ggtggtggtg gggattgtga
       61 tgagtgggtg ggcctcgaac aacaaatggt ctctgcttgg cgggatgcgc agtgccgcgc
      121 agattgtgag ttacgaggtt cctgtgagtc tcacggtgct gatggttgtc ttgtggtgtg
      181 gctctctgaa tatcaaagaa atcattttgg cgcagggggc ctggccgtgg caatggcacg
      241 tgtttaaaac gcctttcctg tttgtggcgt tttttgtttt tttcatcggt tcactcgccg
      301 agggcaaccg cacgccgttc gatatggccg aggcagaatc ggaactggtg agcgggtaca
      361 atacggaata ttccggattc cgttttatcc ttttcttttt tgccgaattt gccaacttgt
      421 atctgatggg cgcgatttcg gcgatcttgt ttttgggtgg ttggaacgca ggtgtggtgc
      481 cggagtttgt tattaaaact ttgggagtgg aaacgccgtg gctggcctgg ctcatcaaaa
      541 atctgattga gcatatcgtg tttcagacga agtcgctggg tttggtgttc attatcatcc
      601 aactcagatg gacgctcccg cgcgtgcgga tcgatcagct gatgggtttg tgctggaagt
      661 atctggtgcc gatcgcgttt gtgaatcttg tgggcatgtc gctgtggatg gtgtttgtga
      721 cgcctaaggt cacccagtgg gttgcctggg ggatgactgg gcttggtggg ttgattgcgt
      781 tgcattttat cagacgcgtg gcctggcatt tgaggtacac caaggcaaaa ctgaggtgga
      841 atccgtttgt atagaacgtg tttacgaggg gaaaggtgaa aggtgaaagg tgaaaggact
      901 tccgctaaca gctaacagcc aacagctgac ggccgaagat aatttaatgg ctgtaggctg
      961 tcgaccgtaa gccgtcagca gaatgctttc ttttgagctt tgagctgagt catgtcttat
     1021 ttgtccaaca tagtttcctc tgttaaaacg gtctttgaag ggatgacgat cacgttctcg
     1081 cacctgtttc gtgaacccat gacgattcag taccccgatc gaacacccag accagtcaag
     1141 gaaactctgc cccagcggta tcgtggtttt ctcgcgacag atatgagtat ttgcacaggg
     1201 tgtacgctgt gccaaaaggc gtgcccaatt gactgcatcg tgatagaggc caccaaagat
     1261 cccgaaaagg gacgtgtgct gactcgtttt ggaatcgatt tgggcaaatg catgtattgt
     1321 gggctgtgtg ttgagccttg ccccacgggt gccattgctt tcacgaggga gtttgagggg
     1381 gccacgcctg atctcaaaac attgcaacgc gagtttattc gacaggggga atgcgtggtg
     1441 ccgtttaaaa aggcgtaagg cataaggcgc aaggcgtagg gcggatacgt tggggtaagg
     1501 gaaaagatcg caatgacagt acgatccacg attcacgatc gtcgatccac gagttattat
     1561 ggaacagatt attttcatcc tcttctttgt catcaccatc gggtcggcct gtgtggtggc
     1621 ctttgggcgg aatctcgttt acagtgcgtt tgccctgctg ggcacttttt tgggtgtggc
     1681 ggggattttc attcttctgc acgcggagtt tttggctctg gtgcaagtta tcgtgtacgt
     1741 tggggggatt ctcgtgttga cagtctttgc cgtgatgctc acctccaaga ttcatgcgtc
     1801 acaaaccaca aaccccgcac tcaatctcaa a
//
LOCUS       DATDUN010000085         1148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_254028, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000085 DATDUN010000000
VERSION     DATDUN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254028"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="purD"
                     /locus_tag="VJC18_01245"
     CDS             complement(<1..799)
                     /gene="purD"
                     /locus_tag="VJC18_01245"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HLD44031.1"
                     /translation="MKVLIIGSGGREHALLKKVVVSPKVKKIYCVPGSSALEETAECV
                     FIPPDNIGMLVDFAKHHKIDLTIVGPELPLTKGIVDAFEAEQLKIFGPSRDASQLEGS
                     KVFCKQFCQRHGIPTAGYREFEDFASAKEYLLSGLGYPVVIKTDGLAAGKGVSIVQGP
                     DEGLKIINDMMVYEKFGEAGRRIIVEEFLRGAEASFIVVTDGSRFVSFPAAQDHKAVF
                     DGDVGPNTGGMGAYAPARVFDEKIRQQVIATIIQPTIDGMRKDNKPYV"
     gene            complement(810..>1148)
                     /locus_tag="VJC18_01250"
     CDS             complement(810..>1148)
                     /locus_tag="VJC18_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44032.1"
                     /translation="ALNQEESIVKDLPTVPRTTIKVSEPVTAPVTQEVAQEDTPPIPP
                     ARRTVQFETFNDGDDDFDDITNISDIQIVNSENEGDVILVDFDEEKPDLASKFRRKLR
                     DKRRKIKIGF"
BASE COUNT          274 a          327 c          260 g          287 t
ORIGIN      
        1 ccacgtaggg tttattgtct ttccgcatgc cgtcgatcgt gggctggatg atcgtggcaa
       61 tcacctgttg cctgatcttt tcatcaaata ccctggcggg agcgtaggcc cccatcccac
      121 ccgtattggg accaacatcg ccatcaaaaa cagccttatg atcctgcgcc gcgggaaagg
      181 aaacaaagcg ggagccatca gttaccacaa taaaggaagc ctccgctccc cttaagaatt
      241 cttccacgat gatgcgcctg ccagcctcac caaatttttc gtaaaccatc atgtcgttga
      301 tgatcttaag tccctcatca ggaccctgca caatgctgac gcccttgccc gcagccagcc
      361 catccgtttt gatgaccacg ggatacccaa gtcccgacaa cagatactcc ttcgccgaag
      421 caaaatcctc aaactcacgg tacccggcgg tcgggatgcc atgacgctgg caaaattgtt
      481 tgcaaaaaac ctttgacccc tccagttggc tcgcatcacg actgggacca aatatcttca
      541 actgttcggc ctcaaacgca tccacaatcc ccttggtcaa cggcaactca gggccaacaa
      601 tggtgagatc aattttgtgg tgtttggcaa aatcgacgag catgccaatg ttgtctggcg
      661 ggataaaaac acactcagca gtctcctcca gggccgatga cccaggcacg caatagattt
      721 tcttcacctt gggtgaaaca acgaccttct tcagaagggc atgttcgcgt ccaccactgc
      781 cgatgattag taccttcatg cgtcacccct caaaatccta tttttatttt tctgcgttta
      841 tccctgagct tgcggcgaaa ttttgaggcc agatccggtt tttcttcgtc gaaatcaacc
      901 agaatcacat cgccctcatt ctcggaattc acgatctgga tgtcagagat gttggtgatg
      961 tcatcaaaat catcatcgcc atcattgaat gtttcgaatt gaacggtacg tctggccggg
     1021 ggaatgggtg gggtgtcttc ttgggcaacc tcttgtgtga caggagccgt gacaggttcc
     1081 gaaactttga ttgtcgtccg tggcacagtc ggcaagtcct tgacgatact ctcttcctga
     1141 ttcagcgc
//
LOCUS       DATDUN010000086         1532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_211764, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000086 DATDUN010000000
VERSION     DATDUN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211764"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /gene="priA"
                     /locus_tag="VJC18_01255"
     CDS             complement(<1..1290)
                     /gene="priA"
                     /locus_tag="VJC18_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HLD44033.1"
                     /translation="MKDNNQLDCVLCSDTGYVNVVVAVPLTTLFTYRVPRSLRGQLTR
                     GKRVLVPFGRKQIIGICWDVMTTLPASVPQKQQIKDILTVLDEQPLVDAAYLQWLEFA
                     ADYYATPLGQVLSQAIPSLYLDVQKIGKEHSYKYKPINFSHDFSTKDVVLTNEQNLVY
                     QSLAGHLDEYYPVLLRGVTGSGKTEVYIRVIKTVLQQNKSALFLVPEIGLTPQMLARL
                     NHHFAGSLLVYHSSLTQNQRLSQWQSCLDERPQVLVGTRSALFAPFKNLGVIIVDEEH
                     DHSYKQEERFRYQARNLAVSRARILKIPVIMGSATPSLETYYQVINNKYHLYELHARS
                     GGASLPDIRVIHFGKEREQRKTPLCLSQHIHDAIEHFHKKKKQMLIYVGQRGFAQSAY
                     CASCATIQICPNCSVGLKYHKEGRVLKCHYCDFAKPYD"
BASE COUNT          394 a          390 c          365 g          383 t
ORIGIN      
        1 atcatacggc tttgcaaagt cacagtaatg acatttgaga acccttccct ccttgtgata
       61 cttgagcccc acactgcaat tgggacaaat ctggatggtc gcgcaggacg cacaatacgc
      121 actctgcgca aaccctcgct gaccaacata aatcaacatc tgttttttct ttttgtgaaa
      181 gtgctcgatg gcatcatgga tgtgctgaga aagacacagg ggcgttttgc gctgttcacg
      241 ttccttgcca aaatgaatca cgcgaatgtc tggcagactc gcgccacctg aacgggcatg
      301 cagttcatac aaatggtatt tgttgttgat gacctggtaa tacgtctcca aactgggcgt
      361 ggccgatccc atgatgaccg gtatcttgag gattcgtgct cggctcacgg ccagattgcg
      421 ggcctgataa cgaaaccgtt cctcctgctt gtaggaatga tcatgttctt catcgacgat
      481 gatcaccccc agatttttaa agggggcgaa cagggccgaa cgagttccca caaggacttg
      541 cggtctttca tccagacaac tctgccactg gctgagccgc tgattctgtg tgagcgagct
      601 gtgatacacg agcagggatc ccgcaaaatg atgattgagc ctggccaaca tttgcggtgt
      661 gagcccgatt tcggggacca gaaacagggc cgatttgttc tgctgtaaaa cagttttgat
      721 cacccggatg tagacctctg ttttcccaga acccgtgacc ccacgcagca agacaggata
      781 atattcgtca agatgccccg ccagactttg gtacacaagg ttttgctcgt tagtcagcac
      841 cacatctttg gtggaaaaat cgtgactgaa attgatgggt ttgtatttat aagaatgttc
      901 cttcccgatt ttttgcacat ccagatacag ggaagggatg gcttgcgaca aaacctgacc
      961 caagggtgtc gcgtagtaat cagcggcaaa ctccagccac tgcaggtacg ccgcgtctac
     1021 cagcggttgc tcatcgagca cggtcaggat gtccttgatc tgttgcttct ggggcacaga
     1081 ggcgggcaaa gtggtcatca cgtcccagca aatcccgatg atctgtttgc gtccaaaggg
     1141 caccaagacc ctttttccac gcgtcagctg ccctctcaaa gaccttggca cacggtaggt
     1201 aaacaaggtg gttaacggca cggcaacaac gacattgaca tagcccgtat cagagcacaa
     1261 aacacaatct aactggttgt tatctttcat aaataatagg tccaaaacca ttgaatctct
     1321 attgacatcg ggccttaatg tttctatttt gcgagttcgt tcaaccacat taactgattt
     1381 gtcgcaatga catttgaaag attgttatgg atactgtcac cgaaataaaa gaacctgcaa
     1441 aaaacatcat cggcatcaat ctcgaagacg agatgcgcga ctcttatctg gattattcca
     1501 tgagcgtgat catcggccgc gccctccccg ac
//
LOCUS       DATDUN010000087         1872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_378078, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000087 DATDUN010000000
VERSION     DATDUN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378078"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VJC18_01260"
     CDS             complement(<1..723)
                     /locus_tag="VJC18_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44034.1"
                     /translation="MNMRTFFFIFLVLLSLSAGHALANIPDSTLGLIPDPPADQARAY
                     EQHASQDGAFGDYFRLRVAEKHLKAGNLNEAFRFVSRVHDPLFIFWKKVLLAEIYWAR
                     EQVRQSLALMGSLPPKPDYELFYGEGLYEQLFKRAVLIRLLAKKALNQNTREDTALIV
                     TLFPDDKMLTDSLGEQAENSGLNTTDKITRLNTLSKKRMLKLIPGLVSAQEIEDAHLP
                     LENKCTSLFALGNATRLSKDHWT"
     gene            968..>1872
                     /gene="recF"
                     /locus_tag="VJC18_01265"
     CDS             968..>1872
                     /gene="recF"
                     /locus_tag="VJC18_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HLD44035.1"
                     /translation="MQLKTLRLKNFRNIREQAFVFHPEFNFIFGRNAQGKTNIIEAIY
                     FLAELKSFRTTNRHDIISHGADCAKIQGVFEKDELSWDIDISLTGQERRVLLNSKKPG
                     HRQDYYALIPLILFEPRHIYLFRDSPSERRRYMNRALYVQDVTLLGLFQDYDKVVAHK
                     NRLLKEGAPDALLCVWNDRLISLASQIIYKRLRWMEQMSQVLTQEYRSISNSADDLRI
                     TYLSTVKALNDNVVTLGEQELAILLQEELRIMQHRERERRESLVGPHRDDFNIFVGER
                     SVGALGSQGENRSVVIALKLSQLKL"
BASE COUNT          454 a          427 c          501 g          490 t
ORIGIN      
        1 ggtccagtgg tctttgctca atcgggtggc atttcccagg gcaaacaatg atgtgcactt
       61 gttttcgaga gggaggtgtg cgtcttcgat ctcctgggcg gataccaatc ctggaatcag
      121 cttcaacatc ctttttttgg atagtgtgtt gagtctggtg attttgtcgg tggtgttgag
      181 gccactattt tctgcctgct ctcccaagct gtcggtaagc attttatcat caggaaacag
      241 tgtcacgatc agggctgtgt cctcgcgtgt gttttggttt agggcttttt tggccaacaa
      301 cctgatcaag acagctctct taaagagttg ttcgtatagg ccttcgccat agaagagctc
      361 atagtccggt ttgggtggca ggcttcccat caaggccagg ctttgacgga cctgttcccg
      421 tgcccaataa atctcggcca gaaggacttt tttccaaaaa ataaagaggg gatcatgcac
      481 ccggctaaca aaacggaagg cctcattcaa gttgcctgcc ttgagatgtt tttcggcaac
      541 acgcaaacga aaataatccc caaaagcgcc atcctgagag gcgtgttgct cgtaggcccg
      601 tgcctgatct gctggggggt cggggatgag tcccagcgtg gaatcgggaa tgttggccag
      661 tgcgtgtcct gctgaaagac ttagcaaaac aaggaaaatg aaaaaaaatg tgcgcatgtt
      721 catgtgttta cgaaaagggc ctggggcgta gggctcaagg cttagggaaa gacgaggaag
      781 atgaatgtgc cgtttttttg ccttacgcct taaaccttgg gccttatgcc ctcactttct
      841 cacgtatatg gtacgcacgc acgcacgtca acacgtacgc acgcaaattg tgtgcctgtt
      901 catttttgtt ttgcaagcgc ggggaaatat tctttacttc aaaatatgaa aacaataagg
      961 atgccacatg cagctcaaga ccttgcggct caaaaatttt cgcaacatca gggaacaggc
     1021 ctttgttttt caccccgaat tcaacttcat ctttggcaga aacgcgcagg gcaagaccaa
     1081 tattatcgag gccatttatt ttttggccga gctcaaatcg tttcgcacaa cgaatcggca
     1141 cgacatcatc tcacacgggg ctgactgcgc taaaattcag ggtgtgttcg aaaaagacga
     1201 attgagttgg gatatcgaca tttcacttac ggggcaggaa cgacgcgttc tgcttaactc
     1261 caaaaaaccc ggtcatcggc aggattatta cgcgctgatt cccctgatcc tgtttgaacc
     1321 gcgacacatc tatcttttta gagactcccc atcagagcgg cgacgttaca tgaatcgggc
     1381 gttgtatgta caggacgtga ccttgcttgg tttgtttcag gactacgaca aggtggtggc
     1441 acataaaaac agactcctca aggagggggc tccggatgct ttgttgtgtg tttggaacga
     1501 ccgactgatc agtttggcct cgcaaatcat ttacaaacgc ctgcgttgga tggagcagat
     1561 gtcgcaggtc ttgacgcagg aataccgctc gatcagcaac agcgcggatg atttacgaat
     1621 cacttattta tccacggtca aagctctcaa cgataatgtg gtgaccctgg gagagcagga
     1681 gcttgccatt ttgttgcaag aggagcttcg catcatgcag cacagagagc gcgagagacg
     1741 agaatctctg gtggggcctc atcgggacga ttttaatatt tttgtggggg agcgcagtgt
     1801 gggtgctttg gggtcgcagg gcgaaaacag gtctgtggtg attgcgctca agcttagtca
     1861 gctcaagctg tt
//
LOCUS       DATDUN010000088         1065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_543486, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000088 DATDUN010000000
VERSION     DATDUN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543486"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VJC18_01270"
     CDS             <1..255
                     /locus_tag="VJC18_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HLD44036.1"
                     /translation="LANPLSFSSCSHGAGRKMSRTKAKDRFSPADLARQTQGVECDKS
                     GRLLEEIPAAYKNIDEVMAAQSDLVHSVTRLKQIITVKSY"
     gene            complement(311..565)
                     /locus_tag="VJC18_01275"
     CDS             complement(311..565)
                     /locus_tag="VJC18_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44037.1"
                     /translation="MSGLQIDWLIMRRDGVWNVLECKFHTQPVGTWVIDDVIQKIKLL
                     NSPDSVTLEPILIASHGVTKEVLKKGFFNQVITLKELLVP"
     gene            814..>1065
                     /locus_tag="VJC18_01280"
     CDS             814..>1065
                     /locus_tag="VJC18_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HLD44038.1"
                     /translation="MFIGRNEELQRLKEELGRKTACLIVCRGRRRIGKSRLIEEFGTL
                     AENFYAFQGLAPNEGVTSREQLKNFAEQISQNFDLPEIMP"
BASE COUNT          299 a          250 c          245 g          271 t
ORIGIN      
        1 ttggcgaacc ctttgagttt ttcctcatgt agccatgggg ccggacgcaa aatgagtcgg
       61 actaaagcga aagatagatt cagtccagca gatttggcca gacaaaccca gggcgtggag
      121 tgcgataaat cgggcaggtt gctcgaagaa atccccgccg cctacaaaaa tatcgacgaa
      181 gtcatggccg cacagagcga tctggttcac agcgtgaccc ggctcaaaca aatcatcact
      241 gtgaagagtt attgatattt gagctttatt gacgacatca tgaccctgac gacgtggcag
      301 gttttggctt tcaagggacc aagagttctt tcagcgtgat gacctggttg aaaaaccctt
      361 ttttcaacac ctcttttgtg actccgtgcg aggcaatcag aatgggttca agcgttacgg
      421 aatctggtga attgagaagt ttgatttttt gaatcacatc gtcaatcacc caagtgccca
      481 cgggttgggt gtgaaatttg cactccagaa cattccacac gccgtctctg cgcatgatca
      541 accaatcaat ttgcagtcca ctcacattgc caaatcatga gcaacaggaa cttaagggca
      601 tctctaaaaa cccccgtgtg acccccctct tgtaaacagg gggttcttag ggcctttatt
      661 tctcctgtca ccaaaacgat gttggtttat cgctttgttt tgcacctgaa aacaaagcga
      721 taaacgagca atatgtcgat ttagcgctaa atttataagg ctattattca tcgaacattt
      781 gacatattgt tgatttgccg ctaactacag actatgttta tcggacgaaa cgaagaactg
      841 caaaggctca aagaagaact gggacgcaaa acagcttgtt tgattgtgtg ccgcggacgt
      901 cgtcgcattg gcaagagccg cctgatcgaa gagtttggaa cccttgcaga aaatttttat
      961 gcctttcagg ggttggcccc caacgagggg gtcacaagca gagagcaact caaaaacttt
     1021 gccgaacaaa tctctcaaaa ttttgatctc cccgaaatca tgcca
//
LOCUS       DATDUN010000089         1208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_254171, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000089 DATDUN010000000
VERSION     DATDUN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254171"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VJC18_01285"
     CDS             <1..392
                     /locus_tag="VJC18_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44039.1"
                     /translation="TAPSELWNINAGKFSIGLNHNAVLDAVVTLKKSWYDVTTAQEAY
                     EKRKNNLSSYLPGAIFIMENQRVLLPGNIEALSMTYKNPSDLKVSREIMFVHRGAAYE
                     FVFQAKEENFTTVKEDFRYILENMKLY"
     gene            complement(786..>1208)
                     /locus_tag="VJC18_01290"
     CDS             complement(786..>1208)
                     /locus_tag="VJC18_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit beta"
                     /protein_id="HLD44040.1"
                     /translation="KLGPKKNMIQILSAHKVPYCATANVAWPEDLTAKVRKAASMKGT
                     RFLHVLSPCPPGWRISSEHGIEIARLATNTKVFPLYEVVDGTKYHITYQPKNTPVRDY
                     LMAQGRFRHLKDEDITHIQAQVESDWERLKQMEQISSE"
BASE COUNT          279 a          297 c          271 g          361 t
ORIGIN      
        1 ttacagcccc gagcgaacta tggaacatca atgccggcaa attttccatc ggtctcaacc
       61 ataacgctgt tttggatgcc gtggtgacgc tcaaaaagag ttggtacgat gtgaccacgg
      121 cccaggaagc ctacgagaaa agaaaaaaca atctatcctc atacctgccc ggagccattt
      181 tcattatgga gaaccaacgg gtcctcctgc caggcaatat cgaagcactg tcgatgacgt
      241 ataaaaaccc cagcgacctg aaggtctccc gcgagatcat gtttgtgcac cgcggtgcgg
      301 cctatgaatt tgtttttcag gccaaggaag aaaatttcac cacggtcaaa gaagatttca
      361 ggtacattct ggagaatatg aagttgtatt gattcagacc catcatcaaa aatcgtgatt
      421 ttttatatca ttttgtcttt ctgcacaata tgccttgcac aacacttatg ctttcgtgat
      481 agcatcgcaa cagattatca ccccagtcgc ttcggcgctg ggcgcgaggc cgctttttag
      541 cggcctttgc tttttctatg agccacaaat caaaaacctt tgtttatatc gacgccttta
      601 atctctatta cggtgccatc aagggcacct ctacacgact ctgaaatccc ctttgccttc
      661 tttttttgct ttcttggcaa gtcttttctc tttaagggtt ttttggggtt tcttttttgc
      721 atcttttttt gcgttttgat ctttactcat gttttttcct ttgtttgtga aaaatctcga
      781 taatattatt cacttgaaat ctgttccatc tgctttaatc gctcccaatc agactcgacc
      841 tgagcctgga tgtgggtgat gtcttcgtcc ttcagatggc gaaaacgacc ctgtgccatc
      901 aggtaatcgc gcacgggcgt gttcttgggt tggtacgtga tgtgatactt cgtgccatca
      961 accacttcgt aaagagggaa aactttggtg ttggttgcca aacgggcaat ctctatgcca
     1021 tgctcggatg aaatgcgcca gcccggcggg cacggtgaca gcacgtgcag aaaccgcgtt
     1081 cccttcatcg atgcggcttt gcgaactttg gccgtgaggt cttccggcca ggccacgttg
     1141 gctgtggcac aatacggcac cttgtgcgcc gacaaaatct gaatcatgtt ttttttgggg
     1201 cccagttt
//
LOCUS       DATDUN010000090         1321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_254240, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000090 DATDUN010000000
VERSION     DATDUN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254240"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VJC18_01295"
     CDS             <1..324
                     /locus_tag="VJC18_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44041.1"
                     /translation="VRLLKFNEYPQDWNPEKDQRTPIWEALHHMIRMFQSGGENAAGT
                     LLAQMPARAENMRQLAYRLYTLCERKGWAEDARAYNELITSWAQIETESHKIGHVGTQ
                     RELEF"
     gene            325..>1321
                     /locus_tag="VJC18_01300"
     CDS             325..>1321
                     /locus_tag="VJC18_01300"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HLD44042.1"
                     /translation="MEEIVQQILDIPAENMTLEFKRLAGDGVVSKCVETITAFANTEG
                     GVLILGVDDPEKTKLKGVKRIYGIEEHLENYDAIGRELQRVIPSMVGVWPPAKFAVAE
                     AEGRTIGLVHVPKAAQQFYVCNNRVYVRQEKGNKPPLTPQEIVKLSYAKGFVFADGEL
                     VDVSFELFETESYKTWRRNRGQTTDIKTEQDLLEVGLARRHTDGKIQPTRAAVMLFCN
                     HPTYLMETKCAIRVFQYEGTIEKIAEVPNLISTPKTLEGPIIKLIQDAHAYVLSLLRD
                     GVRLASGFVNKYQIPERAITEAITNAVIHRDYHLQRDIEVNLFEDRIEIESPGLLP"
BASE COUNT          407 a          255 c          328 g          331 t
ORIGIN      
        1 gtcagactct taaaatttaa cgaataccca caagactgga atcccgaaaa agatcagcgc
       61 acgcccatct gggaagccct gcatcacatg attagaatgt ttcaatccgg tggtgaaaat
      121 gccgcgggga ccctattggc acaaatgcct gctcgagccg aaaacatgag acaattggct
      181 tatcggcttt atacactatg tgaacgtaaa ggctgggcag aagacgcgcg ggcgtataat
      241 gaacttatca cctcctgggc acagattgaa acagagtctc ataagattgg gcatgtgggc
      301 acacagagag aattggagtt ttaaatggaa gaaattgtac agcaaatttt ggacattccc
      361 gcagaaaaca tgactcttga gtttaagaga ctcgctggtg atggtgtggt cagtaagtgc
      421 gttgaaacta tcaccgcgtt tgcaaatacc gaaggtggtg ttttgatcct gggcgttgat
      481 gatcctgaaa aaacaaaact taaaggagtt aaaagaatct acggcattga agagcatctt
      541 gaaaattatg acgccatagg gcgagagttg caacgagtca taccttcaat ggttggtgtc
      601 tggccacctg caaaatttgc tgtggcagag gcagaaggac ggactattgg gttggtgcat
      661 gttccaaaag ctgcacaaca gttttatgtt tgtaacaata gagtctatgt ccgtcaggaa
      721 aaaggcaaca aaccacccct caccccacag gaaattgtta aactgtctta tgcaaaaggt
      781 tttgtgtttg ctgatgggga gctggttgat gtgagttttg agctgtttga aaccgaaagt
      841 tataagacgt ggagaagaaa tcgtggtcaa acaacggaca taaaaactga gcaagattta
      901 ttggaggttg gattggctag gaggcacacc gatggcaaaa tccagcctac ccgtgcggca
      961 gtgatgttgt tttgtaatca tccgacgtat ttgatggaaa caaaatgcgc gattcgggtt
     1021 tttcaatatg aggggacaat tgaaaaaatt gccgaagtgc cgaatctcat cagcacaccc
     1081 aaaaccttag aaggccccat tattaaactg atacaagacg cacatgccta tgttttgagc
     1141 ttgttaagag atggcgtgcg gctggcatct ggttttgtaa acaaatatca aataccagaa
     1201 cgggctatta ccgaggccat taccaatgca gtgatacatc gagattatca tttgcaacgt
     1261 gatatagagg tcaatctttt tgaagatcga attgaaatag aaagtcccgg gttactcccc
     1321 t
//
LOCUS       DATDUN010000091         2566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_88878, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000091 DATDUN010000000
VERSION     DATDUN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88878"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VJC18_01305"
     CDS             <1..417
                     /locus_tag="VJC18_01305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLD44043.1"
                     /translation="DFLDALDRIIKKAETADSHVAVIILGLANLSEIQALCTEKDVAW
                     LFNDMSREIRNKALRATDFFHFRRDRQEFILVFADTEAGTAQTIIRRIHNLTKDCFCP
                     GKKGKIYVTLKSGFAVFPDDANNPRDLIHCALQGMK"
     gene            428..1039
                     /locus_tag="VJC18_01310"
     CDS             428..1039
                     /locus_tag="VJC18_01310"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HLD44044.1"
                     /translation="MNRFKSFIKSTIIGGITVILPIALLVMIFKWLFKITTNLIQPLT
                     NFFTKKVPMQEFLGDAIVIAIILLVCFVVGAIIRTKVGRFLHLSLEENLLKFAPGYNM
                     IKETILQFLGKSKPPFSQVCLVQIFENSTMCTGLIAATHENGYYTVFTPTGPNPTTGI
                     IYHVKKEFVHIVDVPIETAVRTFISCGVGSERVMRGLPPLSDK"
     gene            1228..1911
                     /locus_tag="VJC18_01315"
     CDS             1228..1911
                     /locus_tag="VJC18_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44045.1"
                     /translation="MKNYFMQFLVGLIISGLVFIPVETNAGMTGFKINNQEVRVIPKA
                     QLQDKGFDTGSAYIDFNSEESPVDQIAKNVGDNADVLACFEELIAQQQMGDYMLKAPK
                     GLLVSSQKALKTVTQTCDDITVQFVPAYQNIEGISLDTLASNDSNIFRHMSDFFGIKE
                     AQASALLTIGVAVALGAVIGWVARGKVDKKKDKKNESSDDTTEADDDDDDATDTPTTE
                     SADCPGESC"
     gene            complement(1986..>2566)
                     /locus_tag="VJC18_01320"
     CDS             complement(1986..>2566)
                     /locus_tag="VJC18_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44046.1"
                     /translation="TGTYQFIKDEWADVEDTKAALEMYRTALGQIGWASDNGAIDQCL
                     ANFDAMAVAAEEIAQDNMDQYTDAIDSAINAVKNDWIAYFEIGTWLFDGITGDHWKYG
                     VVGALDALKGATTVDEAYLALQTHFATVNAAWDGDILASVIVHLKQTESGKALLKQFK
                     AMDGFKTWAAGIINGEKDKPYHADGALNFLYS"
BASE COUNT          688 a          638 c          541 g          699 t
ORIGIN      
        1 gattttctcg atgctctcga ccgcatcatc aaaaaagcgg agacagcaga cagccacgtg
       61 gccgtgatca ttttaggatt ggccaacctc agtgagattc aagcgttgtg taccgagaaa
      121 gacgttgcct ggctgttcaa cgacatgtcc cgcgaaattc gcaacaaggc cctgcgcgcc
      181 acggattttt ttcacttcag gagagaccga caagaattca ttctggtttt tgccgacaca
      241 gaagccggta ctgcgcaaac catcatcagg cggattcaca acctgaccaa agattgtttt
      301 tgccccggaa aaaagggcaa aatctacgtc acgttaaaat cgggttttgc cgtttttcct
      361 gatgatgcca acaacccgcg cgacctgatc cactgtgccc tgcagggcat gaaatgagca
      421 cacctgtatg aaccgattca aatcatttat caaatccacc atcattggcg gcattaccgt
      481 gattttgccc atcgccttgc tggtgatgat tttcaagtgg ttgttcaaga tcaccaccaa
      541 cctcatccag ccactcacca atttttttac caaaaaagtc cccatgcagg aatttctggg
      601 tgacgcgatt gtgatcgcca tcatccttct tgtttgtttt gttgtggggg ccatcatcag
      661 aaccaaggtg gggcgttttc tgcacctgag ccttgaggaa aacttgctca aattcgcgcc
      721 ggggtacaac atgatcaagg aaacgatctt gcagtttctg ggcaaatcca aacccccctt
      781 ttcccaggtt tgtctggtgc aaattttcga aaacagcacc atgtgcaccg gtttgattgc
      841 cgccacacac gagaatgggt actacacggt tttcacaccc acgggtccca atccgacgac
      901 tggcatcatc tatcatgtca aaaaagagtt tgtgcatatt gtggacgttc ccatcgaaac
      961 cgccgtgcgt accttcatca gttgcggcgt ggggtcggag cgggtgatgc gggggttacc
     1021 cccgctttct gacaaatagg gcgcaggtta cccaaattgt ttctcaatct gataagattt
     1081 ttcatttttt tacctttttt gacgcttatt ttcctcaatc attatttttt atcttcataa
     1141 tctgccataa ttgttgttat tatttttaaa gtatctggca ccggttttgc atgataatgt
     1201 tcacataaca ttaatggagg tgtttttatg aagaattatt tcatgcaatt tcttgttggt
     1261 ttaattatca gcggactcgt ttttattcct gttgaaacaa atgctgggat gacgggattc
     1321 aagataaaca atcaagaagt tcgggtcatt ccaaaagctc agcttcagga caagggtttc
     1381 gacacgggat ccgcttacat tgattttaac agcgaagaat cccccgtgga tcaaattgca
     1441 aaaaacgttg gagacaatgc cgatgttctt gcctgttttg aggagctaat cgcccaacaa
     1501 caaatgggtg attatatgct caaggctcct aaaggcctgt tagtgagttc tcagaaagcc
     1561 ctcaagaccg tcacacaaac ctgtgacgac atcacggtac agtttgttcc tgcctaccaa
     1621 aatattgagg gcatcagtct ggatacctta gccagcaacg attccaacat ctttcgtcac
     1681 atgagtgatt tcttcgggat caaggaggca caggcaagtg ctttactcac catcggcgtt
     1741 gctgttgccc ttggcgctgt cattggatgg gttgccagag gaaaagtcga caagaaaaaa
     1801 gataaaaaga atgaatcttc tgatgatacg acagaagcag atgacgatga tgatgatgcc
     1861 actgatactc ctacaacaga aagtgcagat tgtccgggag aatcatgtta attttataaa
     1921 attgaataag gaaaaatccc gctcccatat aaggacgcgg gatttttttt tgaactcaaa
     1981 tctcattaag aataaagaaa attaagcgcc ccatcagcat gatatggttt gtccttttca
     2041 ccattgatga ttcccgccgc ccaagtctta aacccatcca tagccttgaa ctgtttcaac
     2101 agagcctttc cactttcggt ctgtttcaag tgaactataa cgctggccaa aatatcgcca
     2161 tcccaagctg cattgaccgt agcaaaatga gtttgtaatg caagataggc ctcatcgacc
     2221 gtggtggcgc ccttgagggc atccaatgcc ccgacaacgc cgtatttcca atggtcacca
     2281 gttatcccat caaatagcca agtaccaatt tcaaaataag cgatccaatc attcttgact
     2341 gcattaatag cactatcgat agcatccgtg tactgatcca tattatcttg cgcaatttct
     2401 tcagccgcga cggccatcgc atcaaaattc gccaggcact ggtcaatcgc accattatca
     2461 ctcgcccaac caatctgccc caacgccgtg cggtacatct ccaacgcggc cttggtgtct
     2521 tccacatccg cccactcatc cttgatgaac tgatacgtcc ccgtcg
//
LOCUS       DATDUN010000092         1796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_129617, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000092 DATDUN010000000
VERSION     DATDUN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129617"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VJC18_01325"
     CDS             <1..803
                     /locus_tag="VJC18_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HLD44047.1"
                     /translation="TVGCGPLAKFMTQKRITYLKQALTQYSQRLDQKGFVANHDGNLT
                     VKIDSGYLATPTSVPKAFITPEQIITLDFQGKKTAGVGKPFGELNLHLAAYQARPDAQ
                     AVIHAHPPFATAFGLTGQPIKPRLPEAIVSLGGLIPLAPYSMPGSPQNAVAVHEALMI
                     GDVFLIAGNGVLAIGDDLEQAYLRLELVEHLAKIEFYASHLGATMDLPAEDRQALLDK
                     RKTLGLGPQAQLGDIPRPVARPEQKTKDALTDDIKDIIAEEIAKALKQ"
     gene            868..1626
                     /gene="tpiA"
                     /locus_tag="VJC18_01330"
     CDS             868..1626
                     /gene="tpiA"
                     /locus_tag="VJC18_01330"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HLD44048.1"
                     /translation="MSRIPIIAGNWKMNMTLTEAKELVQGIYYSLKFPGQVDVVVAPP
                     FTALAQVSQIAKDTYIDLAAQNLHEEDQGAFTGEVSGRFIKDAGANYVIIGHSERRQY
                     FNETNELIAKKISAALRHELKPIVCVGETLEERETDSHFAVVEDQITGAIKSFNEAEL
                     GHLVLAYEPVWAIGTGKVASIEQIDEIHRHARSVLAGVFTKSFAEKVRIQYGGSVKPD
                     NAKEILSLEHVDGALVGGASLKPHDFVTIIKAAQ"
     gene            complement(1610..>1796)
                     /locus_tag="VJC18_01335"
     CDS             complement(1610..>1796)
                     /locus_tag="VJC18_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44049.1"
                     /translation="DEVKMKELAGPALVKLEPKEWAGGLKGFNITIDTDKLTMADLTK
                     KMLDAGCFNEPATTARP"
BASE COUNT          470 a          507 c          435 g          384 t
ORIGIN      
        1 tgaccgttgg ctgtgggccg ttagccaagt ttatgaccca aaagagaatc acttacctca
       61 aacaggccct cacgcaatat tcgcaacggc ttgaccaaaa aggttttgtg gccaatcacg
      121 atggcaacct cacggtcaaa atcgattctg gttatctggc cacacccaca tcggtaccca
      181 aggccttcat cacacccgaa caaatcatca ctttggattt ccaagggaaa aaaaccgccg
      241 gtgtgggcaa accgtttggt gaactgaacc tgcatctggc cgcctatcag gcgcgtcccg
      301 acgcacaggc cgtcatccac gcgcacccac cgtttgccac tgccttcggt ttgacaggac
      361 aacctatcaa accacggctt cccgaggcca ttgtgtctct gggcggcctc attcctcttg
      421 ccccctactc aatgcccggt tcaccgcaaa atgctgtggc cgtccatgaa gcactcatga
      481 tcggcgatgt gtttttgatc gcgggcaatg gggttttggc cattggtgac gatctggaac
      541 aggcttatct gagactcgaa ctcgtagaac atctggccaa aatagaattt tacgcctcgc
      601 atctgggagc cacgatggac ctgcccgctg aagacaggca agcactgctc gacaaaagaa
      661 aaacattggg actgggccca caagcacaac ttggggatat tccccggcct gtcgccaggc
      721 ccgaacaaaa aaccaaggac gcattgactg acgacatcaa ggacatcatc gccgaggaaa
      781 ttgccaaagc cctcaaacaa taatgttcac atcttcaccg gttcacgaaa atacgtgaac
      841 acgtgaacac gtgaacacgt gaacactatg agtcgcatcc ccatcatcgc aggcaactgg
      901 aaaatgaaca tgaccctcac tgaggccaaa gaactcgtgc agggtattta ttattccctc
      961 aaattccccg gacaggttga tgtggtggtc gccccgccgt tcacagcact cgcccaggtc
     1021 tcacagattg ccaaagacac ctacattgac ctcgcggcgc aaaacctgca cgaagaagat
     1081 cagggggcct tcacgggaga agtctccgga cgattcatca aagatgcggg ggccaattat
     1141 gtgatcattg gtcattccga acgcagacag tattttaatg agaccaacga attgattgcc
     1201 aaaaaaattt cggccgcatt gcgccacgaa ctcaaaccca ttgtgtgtgt gggcgaaacc
     1261 cttgaggaac gagaaacgga ttcacacttt gctgttgtgg aagatcaaat taccggggcc
     1321 atcaagagtt tcaacgaagc agaattgggc catctggtcc tcgcctacga gcccgtctgg
     1381 gccatcggga ccggcaaggt cgcctcaata gaacaaattg acgaaattca ccgtcacgcc
     1441 cgttccgtgt tggcaggtgt tttcacaaag tcttttgcgg aaaaggtgcg gattcaatac
     1501 ggcgggagcg tcaagccaga caacgccaaa gagatcttat cgctcgaaca cgtagacgga
     1561 gcactggtgg gcggagcctc actcaagcca catgattttg taaccatcat caaggccgcg
     1621 cagtagtcgc tggctcatta aaacagcccg cgtcgagcat ctttttggtc agatccgcca
     1681 tggtaagctt gtcggtgtcg atggtgatgt tgaacccttt aagaccgccg gcccattcct
     1741 tgggttccaa tttgaccaag gccggtcccg ccaactcttt cattttgacc tcatca
//
LOCUS       DATDUN010000093         1764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_46066, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000093 DATDUN010000000
VERSION     DATDUN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46066"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VJC18_01340"
     CDS             complement(<1..452)
                     /locus_tag="VJC18_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44050.1"
                     /translation="MKKINRIVFLILVSVSFTLCGGGDSSNVTPPLDLPTYTGEWDNL
                     GGPIGGLGYDVRIDPNDHDIMYVTDNYAGVGKSLDGGATWFRTNSGIDAKSGPTEDSV
                     PIFSLTIDPNDPDIIWAVTDANIAEGLGVYNSTDGGATWTLKDNGISKG"
     gene            complement(496..>1764)
                     /locus_tag="VJC18_01345"
     CDS             complement(496..>1764)
                     /locus_tag="VJC18_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HLD44051.1"
                     /translation="PHQSAKPSHEGENNSCDQCHPEIVTEFKADRHSQSAQNELFLKM
                     FNGTGDRQTGFGYKTDFPNSNGNCASCHQPVAAINQPYHTDPNTVSGVAKEGVNCDFC
                     HKIKNASVDRTGGHPGVLSYQFSRPQTGHQTFYGPLDDVHPGEDSFHPLYAESRYCAP
                     CHNGSFWNTLVYSEYQEWENSDYARQNITCQKCHMPEQKNMTHFALAEKGGLKRVPST
                     LISHKNLGINDVTFMKDSIALKTTAQVVDNKLHVSVAITNTKAGHHYPTGNPMRNMLL
                     LVETKDDRGNVLPLDSGKTLPPWAGQGVREQGNYAGLPGKGFAKILINASPYPGRASR
                     FDFSPITPSPHWRPAYIHEDTRIAANMTDVTDYVFDLPHSVSKTQVTVITKLIYRRTF
                     KNWQNKLDIDLPDLVLATNSQRLTVNSQQP"
BASE COUNT          379 a          414 c          490 g          481 t
ORIGIN      
        1 ccctttgaaa tgccattgtc cttaagcgtc caggtggcgc cgccatccgt gctgttgtaa
       61 acacccaaac cctccgcaat attcgcatcg gtgaccgccc aaatgatgtc gggatcgttg
      121 gggtcaatcg tgagcgaaaa aatggggact gaatcctctg tggggcctga tttggcatcg
      181 atacccgagt tggttctaaa ccaggtggcg ccgccatcaa gacttttgcc aaccccggca
      241 taattatctg tgacatacat gatgtcgtga tcgttgggat cgatgcgtac gtcgtacccc
      301 aggccgccaa tgggtccgcc caagttgtcc cactcgcccg tgtaggtggg gaggtcgagg
      361 ggcggtgtga catttgaact gtctcctcca ccgcagagag taaaggacac agagacaaga
      421 atcaaaaaaa cgattctgtt gatttttttc atattgatcc tttcaaatgt agtcggaggc
      481 ttagcaaaca acaatttacg gctgttggct gttaaccgtt agccgttggc tattggttgc
      541 gagcacgaga tccggtaaat cgatgtccag cttgttttgc cagttcttaa acgtcctgcg
      601 ataaatcaat ttggttatca ctgtgacctg tgtttttgag acagaatgag gcagatcaaa
      661 aacgtagtcc gttacatcgg tcatgttggc ggctattctt gtgtcttcgt ggatataagc
      721 gggtcgccaa tggggagacg gtgtgatggg actaaaatca aatcggctgg ctctgccggg
      781 atagggcgac gcattgatga ggatcttggc aaaacctttt ccgggaagac ccgcgtaatt
      841 gccttgttca cggaccccct gaccagccca gggggggagt gtttttccac tgtcaagcgg
      901 caatacgttt cccctgtcgt ctttggtctc aaccagcaag agcatgtttc tcatggggtt
      961 gccggtggga taatggtgtc cggccttggt attggtgatc gccacagaca cgtggagttt
     1021 gttgtcgaca acttgcgccg ttgttttcag ggcgatgctg tctttcataa aggtcacatc
     1081 attgatgccc agatttttat gagagatgag ggtactggga actcgcttta agcccccttt
     1141 ttcggccagg gcaaagtggg tcatgttttt ttgctctggc atgtggcatt tctggcaggt
     1201 gatgttttgc cgcgcgtagt cactgttttc ccattcctgg tattctgaat aaaccagggt
     1261 gttccaaaaa cttccgttgt ggcagggcgc gcaatagcgg ctctcggcat acaggggatg
     1321 aaagctgtct tcaccgggat gcacgtcatc cagcggccca taaaaagttt ggtgcccggt
     1381 ttgtggtctg ctgaactgat aggacaacac tcccgggtgt ccccctgtcc tgtccacaga
     1441 ggcattcttg attttgtgac aaaaatcaca gttcacacct tccttggcca caccggaaac
     1501 cgtgttggga tccgtgtggt agggctgatt gatggcggct acaggctggt gacaggaggc
     1561 gcagtttccg ttggaattgg ggaaatctgt tttataacca aagcctgttt gccgatcacc
     1621 cgtgccatta aacattttga gaaacaactc attttgggct gattgggaat ggcggtcagc
     1681 cttgaactcc gtgacaattt ctggatgaca ttgatcgcag gagttgtttt cgccctcgtg
     1741 tgagggcttg gcactctggt gcgg
//
LOCUS       DATDUN010000094         2743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_88919, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000094 DATDUN010000000
VERSION     DATDUN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88919"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VJC18_01350"
     CDS             <1..623
                     /locus_tag="VJC18_01350"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HLD44052.1"
                     /translation="SGKNLVGAFYQPKAVIILTDFLKTLPERDYLSGLAEVVKYGLIA
                     DDLLFCTLKNNTGALLNRQHSILTKIINTCCGLKAGIVARDEKDHGLRATLNFGHSLG
                     HAIESLQNYRRTRHGEAIAMGMVFASKLSYALRVSPSDHTSTIALLLTQLHLPTQWPS
                     FSHKDYQRALLIDKKTRDRNIRFILLEKIGRVKSVTLDIKSLLPCL"
     gene            614..1093
                     /gene="aroQ"
                     /locus_tag="VJC18_01355"
     CDS             614..1093
                     /gene="aroQ"
                     /locus_tag="VJC18_01355"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HLD44053.1"
                     /translation="MPVKTAKHKHVLVVHGPNLNLLGSREPAHYGRLTLKDINQKIRA
                     LAKTHGITVSFFQSNHEGAIIDRIQQTQVCDGILINPAAYTHTSIAIRDALLAMNKPF
                     VEVHLTDILSREAFRHHSFFSDIALATVMGRGADSYVEGFVKLVQALKSQGHLSKIT"
     gene            1090..1869
                     /locus_tag="VJC18_01360"
     CDS             1090..1869
                     /locus_tag="VJC18_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44054.1"
                     /translation="MKIALPKISLKADRIKALTKTFMQDKYAKVLHDLTELEIESPDD
                     MRVKQKIGEILYRQGKYDSAITKFDEMVRFFEKNEFILKSIRACKMILKIRPQLIDYN
                     IKLGSLFLKLGMTNESANQYRIVMSHYAAIKDHVNTLKYAKILVKVDPSNDSKVKLAE
                     IYHNSSLVAEAVNLYQELANDARSKKDFDKLQHYYELLLPHVPNNSNLLKDVCILNLR
                     KQKPDYVLKLLDQYQVTDNRAFADLVKKARLMREALRRQKK"
     gene            complement(1871..>2743)
                     /locus_tag="VJC18_01365"
     CDS             complement(1871..>2743)
                     /locus_tag="VJC18_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44055.1"
                     /translation="RGHDSSGRDREDSHVYKERVAKRVVTWFHTVMRSHRLPLIIFIS
                     VLLLLLLSLWPAQAFAETFVKKQFNTLEQEVRSQGTLQEIAKTLSELNHFYEWPKEMS
                     QQLRLYTGIVWFNQNHLTEATTVILATQPDEKYHDLWVYYQAMICLVADHVAKAKPHI
                     EELEKKHGRDVDFLLLKSIYMAQSDNVIGAISIMDNVIRKQKQNGDAYLQRGFFHMMA
                     LSHDLAIRDFAKAAKYQPATEIVKKQQALFQNGLLYIRYKFNEAKGMELIKEGVALDP
                     SSEMVKQMKEALRF"
BASE COUNT          731 a          754 c          608 g          650 t
ORIGIN      
        1 cctcgggtaa aaatctcgtc ggtgcctttt accagccaaa ggctgtcatc atcctcaccg
       61 actttctcaa aacacttccc gaacgcgatt acctgagcgg tctggccgag gtcgtcaaat
      121 acggactcat cgctgatgat ctgttatttt gcaccctcaa aaataacacg ggcgccctat
      181 taaaccgcca gcactcgata ctgaccaaaa tcatcaacac ctgctgtggc cttaaggccg
      241 ggatcgtcgc tcgcgacgaa aaagatcatg ggttgagggc cacgctcaac tttggacaca
      301 gtctgggcca tgccatcgag tccttacaga actatcgcag aacacgccac ggggaggcca
      361 ttgccatggg aatggtcttc gcctccaaac tctcatatgc cctgcgtgtt tcgccgtcgg
      421 accacacaag caccatcgcg ctattgctga cacaattgca cctgcccaca cagtggccat
      481 ctttctcgca caaggattac cagagggcgc ttttgatcga taaaaagacg cgcgatcgaa
      541 atatccggtt tattcttctg gaaaaaattg gtagggtcaa atctgtgaca ctggacatca
      601 agagcctatt accatgcctg tgaaaaccgc caaacacaaa cacgtactcg tcgttcacgg
      661 tcccaacctg aatctgctgg ggtcgcgcga acctgcacac tatggcagac tcaccctcaa
      721 ggacatcaat cagaaaatcc gcgctctggc caaaacacac ggaattaccg tttcgttttt
      781 ccaaagcaat cacgagggag caatcataga ccgtattcaa caaacccagg tctgcgatgg
      841 catcctgatt aaccccgcgg cctacacaca cacgtccatt gccatcaggg acgccttgct
      901 ggccatgaac aaacctttcg tggaggtgca tttaaccgat atcctgtcgc gcgaggcctt
      961 tcgccatcat tctttcttct ccgatattgc gctggctaca gtcatgggcc ggggcgcgga
     1021 cagttatgtg gagggttttg tcaaactggt acaagccctc aagagtcaag ggcacctctc
     1081 taaaataaca tgaaaatcgc tctgccaaaa atcagtctca aggccgatcg catcaaggcc
     1141 ctcaccaaga ccttcatgca ggataaatac gccaaggtcc ttcatgattt gaccgaactc
     1201 gaaatcgaaa gccccgatga catgcgcgtc aagcaaaaga tcggtgaaat attataccgt
     1261 cagggaaagt acgattctgc catcaccaaa ttcgacgaaa tggtgcgctt ctttgaaaaa
     1321 aatgagttca ttctcaagtc catcagggcc tgcaaaatga ttctcaaaat caggccacaa
     1381 ctgatcgatt acaatatcaa gctgggttct ctcttcctca agctgggcat gaccaacgag
     1441 tctgccaacc agtaccgcat cgtcatgagc cattacgccg cgatcaagga ccacgtcaac
     1501 acgctcaaat acgcaaagat tctggtcaag gtggacccgt ccaacgatag caaagtcaag
     1561 ctcgccgaga tttatcacaa ctccagcctg gtcgctgagg ccgtgaatct gtatcaggaa
     1621 ctggccaatg atgcccggag caaaaaagac ttcgacaagc tccaacacta ttacgaactg
     1681 cttttgccgc acgtacccaa taattcgaat ttgctcaaag atgtctgtat tctgaacttg
     1741 agaaaacaaa aaccggacta tgtcttaaag cttctcgacc agtatcaagt caccgacaac
     1801 agggccttcg ccgatctggt caaaaaggcg cgcctcatgc gcgaagccct gcgacgacaa
     1861 aaaaagtaat ctagaatctg agagcctctt tcatctgttt gaccatttcc gatgacgggt
     1921 ccagagcgac gccctctttg ataagctcca tccccttcgc ctcgttgaat ttgtaacgga
     1981 tgtagagcaa tccattctga aaaagggcct gctgtttttt aactatttcc gttgccggct
     2041 gatattttgc ggccttcgca aaatccctga tcgccagatc atgagacaga gccatcatgt
     2101 gaaaaaatcc cctttgcaga tacgcgtccc cgttctgctt ttgttttctg atcacgttgt
     2161 ccatgatact gatcgccccg atcacgttat ctgactgggc catgtaaatg ctcttgagca
     2221 ataaaaaatc cacgtccctg ccatgttttt tttccaactc ctcgatatga ggtttggcct
     2281 tcgcaacatg gtcggccacc agacagatca tggcctgata gtacacccac aaatcgtgat
     2341 acttctcgtc cggctgggtg gcgaggatca ccgtggtggc ctctgtgagg tggttttgat
     2401 tgaaccagac aatccccgtg tacaaccgca attgttgtga catttccttg ggccactcgt
     2461 aaaaatgatt cagctcagac aaggttttgg caatctcttg caaggtgcct tgggaacgaa
     2521 cttcctgttc gagagtgttg aattgttttt tgacgaacgt ctcggcaaag gcctgcgcgg
     2581 gccagaggct caacagcaga agcaataaaa cagaaatgaa gatgatcagg ggaagtcggt
     2641 gcgaacgcat gaccgtatga aaccaagtta caaccctttt ggcaactctt tctttgtaca
     2701 cgtgagaatc ttctctatcg cggcctgagc tgtcgtgacc tcg
//
LOCUS       DATDUN010000095         1958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_46075, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000095 DATDUN010000000
VERSION     DATDUN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46075"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1887)
                     /locus_tag="VJC18_01370"
     CDS             complement(<1..1887)
                     /locus_tag="VJC18_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44056.1"
                     /translation="MHAGASHTAITTTTRKPASTNAPAQDCSSTEGPSTKEFTGQIMR
                     DRANPQNGGFFFHNPTDNDGDKKRVLTVTYKDRNNRDFRLQYATKQTDNPAKSVNSYK
                     ISDVSLEGFTLAENTNTSIPMGDVAHELFKPFTEISGGMVKALVFTRDNCTLTIYLAR
                     IMSTGGGAKHLEPVGYKITGINPETNRPYEEEFNRNLLRMVCDSAQADQYLIEQDDTR
                     GSPHTANIAFKDDAFECETIEGTADFFSLSKDPLDIEKRYGSDPAKQSHHALVVSEEG
                     QIVEIVRTPDGKIERRNEIPQYKATTEGKTKIWQVTLEGSDIQVKHEAGKLMVLERGV
                     VITDNTSCPHRWHRVNKNVVLFVDGRGSPFALQMVDDYLRVISINYETFNNYKYTKHP
                     ISFKTQTAQFVPTVETINILQGAPLTTSKTELFFTGNIGGFEIHEETPLVIRGTIEDG
                     QFIPRVDPTTEVDSAVDLKGTIIGAQIKVKVEKTTDGYQLVLHDGTEEGKKSPIIVPM
                     TFWGKQVVLGTRQEQAIQDIKSILRTSRVDDQPVTVPDEDFVPVTNPQQIIELRSLFT
                     LAELDLARPDTFRQSGAHAKEFSVTFKNGLRLRLVKGQTETHTTFQIFSDPSAPLKLV
                     VNPKS"
BASE COUNT          395 a          431 c          533 g          599 t
ORIGIN      
        1 agacttggga ttgacaacga gcttgagtgg agcagaggga tcagaaaaaa tctgaaacgt
       61 ggtgtgcgtt tcggtttggc ccttaaccag tctcaagcgt agaccatttt tgaaggtcac
      121 agagaactct ttggcatgag cgcctgactg tctgaatgtg tctggtcttg ccagatcaag
      181 ttctgctaac gtgaataatg atctgagttc gatgatctgt tggggattgg tgacgggcac
      241 aaagtcctca tcgggaacgg tgacgggctg gtcatccacg cgggatgttc gcaagattga
      301 tttgatgtcc tggatggcct gttcttgcct ggttcccaga accacctgct ttccccaaaa
      361 ggtcatgggg acgatgattg gcgatttttt gccttcttca gttccatcat gcaataccag
      421 ttggtagcca tcagtggttt tttcgacctt gaccttgatt tgtgccccaa tgattgtgcc
      481 cttgagatcc acagcgctgt ctacctcggt tgtggggtcg actcggggga tgaattgtcc
      541 atcctcaatt gtaccgcgga tgaccaatgg ggtctcctcg tgtatttcaa atcctccgat
      601 gttacccgta aagaagagtt ctgttttgct ggtggttagt ggtgctcctt gcagaatatt
      661 gatggtttcc actgtgggaa caaattgtgc ggtttgtgtt ttgaaggaaa tggggtgctt
      721 ggtgtatttg tagttgttga aggtctcgta gttgatggaa atcacccgga gataatcatc
      781 gaccatctgc agggcaaacg gactccctct tccatccaca aaaagaacta cattcttatt
      841 cacacgatgc catctgtgtg gacagcttgt gttgtcggta atgaccacac ctctttccaa
      901 taccatcagc ttgccggctt catgttttac ctgaatgtcg gaaccttcca atgtcacctg
      961 ccaaattttg gtcttgcctt cagtggtcgc cttgtattga ggaatttcgt ttcgtcgctc
     1021 tatcttgcca tcgggggttc tgacgatctc cacgatctgg ccttcttctg ataccacaag
     1081 tgcgtgatgt gactgcttgg ctgggtctga gccgtatctt ttttcgatgt ccagagggtc
     1141 tttggagagt gaaaaaaaat cagcggtgcc ttctattgtt tcgcattcaa aggcgtcatc
     1201 cttaaacgca atgtttgccg tgtggggact gccacgggta tcatcctgtt cgatcaggta
     1261 ttgatcggcc tgtgcagaat cgcaaaccat gcgcaggaga ttgcggttaa attcttcttc
     1321 gtagggacgg ttggtttcgg ggttgattcc cgtaatttta taaccaacgg gttccagatg
     1381 ttttgcccct ccgccggtag acatgattct ggccaggtaa atggtgagcg tgcaattgtc
     1441 ccgggtgaac accagtgcct tgaccatgcc gccggaaatt tccgtgaagg gcttgaaaag
     1501 ctcatgggcg acgtcgccca tggggataga cgtatttgta ttttcggcca gtgtaaagcc
     1561 ctccaagctg acatcagaaa ttttgtagct gttcaccgat ttggctgggt tatctgtttg
     1621 tttggtagcg tattgtaatc ggaagtctcg attgttcctg tctttgtagg tgacggtgag
     1681 gactcttttt ttatcaccat cattgtctgt ggggttatgg aaaaaaaatc ccccattttg
     1741 tgggttggcg cgatcacgca ttatttgccc ggtgaattct tttgtgcttg ggccttctgt
     1801 tgacgaacaa tcctgcgcgg gtgcattggt ggacgctggt ttgcgcgtgg tcgtggtgat
     1861 tgctgtgtga gaggcacccg cgtgcatggc cggaccacta tctttcggac gggtatgtgt
     1921 ctgtggaacc catgtgtaag gtactgtctc gtgagctt
//
LOCUS       DATDUN010000096         2446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_170450, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000096 DATDUN010000000
VERSION     DATDUN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170450"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1010)
                     /gene="lepB"
                     /locus_tag="VJC18_01375"
     CDS             complement(243..1010)
                     /gene="lepB"
                     /locus_tag="VJC18_01375"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLD44057.1"
                     /translation="MRYFHFLIHLIIIVLVLFIVRGIIYEPFHIPSGSMMPTLLEGDY
                     IGVAKYAYGLRNPFMDGWLIKADGPRRGDVIVFSGPKDQGFDFVKRVVGLPGERVSMV
                     AGQLFINDVAVRMNDIGFKRSAEQDRCQAALMEATTVPDYLLPMPYFREHKNYQIKTE
                     NLPDAPSHVVLVERWGEALRDFEVTVPQEAYFVLGDNRHQSVDSRVFGSVPRASIRGK
                     ATRILFSTNEDDLPCASPFGKEGSKSVRWYRVGKRVC"
     gene            complement(1007..1837)
                     /gene="lepB"
                     /locus_tag="VJC18_01380"
     CDS             complement(1007..1837)
                     /gene="lepB"
                     /locus_tag="VJC18_01380"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLD44058.1"
                     /translation="MFNKQNTPKNPGEKKSPFHIVAEYAQALVGAIILAILIRGFLVE
                     PFKIPSESMVPTLLVGDHIFVARYFYGLRIPFTKKWVTEFKEPQRGDVVVFSYPEDED
                     VDFIKRVVAVPGDTITFDQGVLYVNGEASEYQAFAPEEQDTKNACLIRDTPEMHAVLG
                     DQFKNFPYYLKFKKFRHKLETLPGGTPHMIQRSRKRPNDNGFEAIVPEREYFVMGDNR
                     DHSQDSRFWGFVPRANLKGKAIFIWLPLNHEKTRCRYNLVSPTVFPNVRWDRFGRKIL
                     "
     gene            complement(1907..>2446)
                     /locus_tag="VJC18_01385"
     CDS             complement(1907..>2446)
                     /locus_tag="VJC18_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941921.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HLD44059.1"
                     /translation="IQLCESKRGRQDKIDYITKDRVLVQYEMPFPEIMYDFYDKLKSL
                     TRGYASMDYEVSAYRTSDLIKLNMFINGDPVDALSIISHRDQAYYRGRALAVKLRKLI
                     PRQQFDVNIQAAIGARIIARETVKALRKDVTAKCYGGDITRKRKLLEKQKEGKKRMKQ
                     VGSVEIPQEAFLAALKLDD"
BASE COUNT          626 a          648 c          532 g          640 t
ORIGIN      
        1 ttagagccta tccgaaaacc tgtggataac tgtcgtcccg aagggatgtg ggaatacagc
       61 cgtaggcttt agcctacgga aaacaggcgc cggtaatatt gcaccctgaa agggtgcagg
      121 aaaaatgcca cattgccaac caccgcccct tcagggcgga aaaaatattt cgccacgatt
      181 tccgtgggtt gaaacccacg gctttgttcc accctccctt caggaggttt tcggataggc
      241 tcttagcaca cccttttccc cacccgatac cagcgaacag atttcgagcc ttccttgcca
      301 aaaggactgg cgcagggcag atcatcttcg ttcgttgaaa agagtatgcg ggtcgctttg
      361 cctctgatgg aagccctggg aacggaacca aaaacacgac tgtcaactga ctgatgtctg
      421 ttgtcgccca aaacaaaata ggcttcctgg ggaactgtga cctcaaaatc cctgagcgcc
      481 tcaccccatc tctctaccag caccacatgg ctcggggcat cgggaagatt ctctgttttg
      541 atttggtaat ttttatgctc cctgaaataa ggcatgggca acagataatc aggaactgtt
      601 gtggcctcca ttaatgcggc ctgacatcgg tcttgttccg ctgatctctt gaacccgatg
      661 tcattcatcc tcacggctac gtcattgatg aacaattgcc ctgccaccat actcacacgt
      721 tcccccggca accccacaac cctcttgacg aaatcaaacc cctggtcttt gggaccacta
      781 aaaacaatca catctcctct gcgaggcccg tctgccttaa tcaaccaccc gtccataaag
      841 gggttgcgaa ggccataagc atatttagcc actccgatgt aatccccttc gagcaaggtc
      901 ggcatcatgg aacccgacgg gatatgaaat ggctcataaa taattccacg aacaataaat
      961 aaaaccagca cgataatgat caggtgtatg agaaaatgaa aatatctcaa agaatttttc
     1021 ttccaaagcg gtcccaccga acattgggaa acactgtggg gctaacgaga ttgtagcgac
     1081 atcgtgtttt ttcgtgattc aagggaagcc agataaagat ggccttccct tttaagttgg
     1141 ctctgggaac aaagccccag aatcggctat cctgactgtg atcgcgatta tcacccatca
     1201 caaaatattc tctttcggga acaatggcct caaaaccatt atcgtttggc cttttgcgcg
     1261 agcgttgtat catatgaggc gtgccgccag gcagtgtttc gagtttgtgt cgaaattttt
     1321 tgaatttaag ataataggga aagtttttga actgatcgcc cagaacagca tgcatctcgg
     1381 gcgtatcccg tatcagacag gcgttcttcg tgtcctgttc ttctggggca aaggcctgat
     1441 actcagacgc ttccccgttc acatacaaaa caccctgatc gaatgtgatt gtgtctccgg
     1501 ggacagccac cacccttttg atgaaatcga catcctcatc ctcaggatag gaaaaaacca
     1561 ccacatcacc cctctgtggc tccttgaatt ccgtaaccca ttttttggta aagggaatcc
     1621 tgagcccata aaaataccgc gccacaaaga tatgatcacc taccaggagc gtggggacca
     1681 tcgactcgga aggtattttg aatggttcca ccaaaaaccc tcttatgaga atggccaaaa
     1741 tgatggcccc caccagtgcc tgcgcgtatt ccgccacaat gtgaaaaggg ctcttttttt
     1801 ctccgggatt tttgggtgtg ttctgtttat taaacatgtt tcaggaatat agcgtttttg
     1861 tgggtgcttg taaattacta agaacttggg ttccaagact ctttccctaa tcatccaact
     1921 tcaacgcggc caaaaaagct tcctgcggaa tctcaacgga ccccacctgc ttcattcgct
     1981 ttttgccctc tttttgcttt tccagcaatt ttcgttttct cgtgatgtca ccgccataac
     2041 acttggccgt cacgtctttg cgcaaggcct tcacggtttc cctggctatg atacgggcac
     2101 caatggccgc ctggatgttc acatcgaatt gctgtctggg gatgagtttg cgcaacttca
     2161 cggccaaagc acgtccccgg tagtaggcct gatccctgtg actgatgatt gacaaggcat
     2221 caacgggatc tccattgatg aacatattca gtttgatcag gtcagacgtt cgataagccg
     2281 acacctcata atccatggaa gcataccctc tggtgagcga cttgagctta tcataaaaat
     2341 cgtacatgat ttcgggaaac ggcatttcat attgtaccaa caccctgtct ttggtaatgt
     2401 aatcgatttt atcctgtcgt cccctcttgc tttcgcacaa ctgaat
//
LOCUS       DATDUN010000097         1363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_88996, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000097 DATDUN010000000
VERSION     DATDUN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88996"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VJC18_01390"
     CDS             complement(<1..328)
                     /locus_tag="VJC18_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44060.1"
                     /translation="MSVDRSITKSSSAEYFSKNLQQVGFSSFTKATLTTIKEAVDNSL
                     DACEDHGILPEIFVSVQRLGEGTTRGADLVKVVITDNGPGLTLEDAEKVFGQYLASSK
                     FGRGRCS"
     gene            382..1314
                     /locus_tag="VJC18_01395"
     CDS             382..1314
                     /locus_tag="VJC18_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLD44061.1"
                     /translation="MISASLCYTFNQMPKHSKTKEYFRTLHHKKPVRALTAATLMLAL
                     PFVGAYSYFIGVKRFKIDHIKLEINSPHSQLGGMKIVQISDLHYGPTNKDKRYFNRIV
                     DIINLHQPDLIVLTGDYYQWDPKYLYDLPKMLTRLRAPLGLFGCLGNHDYGSCYPGHT
                     QNDPFEEDITKDALERQGIRILTNEAQLLEYKKQKFNLIGLHDLWSNQCDPNLAFKKV
                     NSKLPTFVLSHNPDSVHIVKHDYDLMLSGHSHGGQVTWPIVGSLAIPQRSKKYSRGLH
                     QINHRKRIYINRGLGHTFRLRINSQPEITLFEII"
BASE COUNT          375 a          384 c          269 g          335 t
ORIGIN      
        1 gactgcaccg accacgtcca aacttggaac tggccaaata ctgcccaaac accttttcgg
       61 cgtcttccaa cgtgagtccg ggaccattat cggtaatcac caccttcacc aaatccgccc
      121 cgcgggttgt cccttcgcca agtctttgaa ctgacacaaa tatttccggc aaaatcccat
      181 gatcttcgca ggcgtccagt gaattgtcga cagcctcttt tatggtggtg agggtcgcct
      241 tggtgaatga tgaaaagcca acctgttgga gattctttga gaaatattcc gctgaactcg
      301 attttgtgat tgatcgatcg actgacatgg gcgctttaat gcctcatgaa ctaccctgat
      361 ggcaagtgaa aaggcatcat tgtgatctca gcctcactat gttatacttt taaccagatg
      421 cccaaacact ccaaaacaaa ggaatatttc cgtaccctgc atcataaaaa acctgtccgc
      481 gccctcactg ccgccacact catgttggcc ctgccctttg tgggggccta ctcttatttc
      541 attggcgtca agcggttcaa aattgatcac atcaagctcg aaatcaacag ccctcattcg
      601 caactgggcg gcatgaagat tgtacaaatc tccgatctgc attacggccc cacgaacaag
      661 gataaacgtt atttcaatcg catcgtcgac atcatcaacc tgcaccagcc ggacctgatc
      721 gtcctcaccg gcgattatta tcagtgggat ccaaaatatc tttacgatct ccccaaaatg
      781 ctcacgcgtc tgcgcgcccc tttagggttg ttcgggtgtt tgggaaacca tgattacggc
      841 agttgttatc ccggacacac acaaaacgac cctttcgaag aggacattac caaagacgcg
      901 cttgagaggc agggaatccg aatcctcacg aacgaggccc agcttctgga gtacaaaaaa
      961 caaaaattca acctgatcgg tttacatgat ctctggtcca atcaatgtga tcccaatctc
     1021 gcgttcaaaa aagtcaacag caagcttccc acctttgtgt tgtcacacaa cccggattcc
     1081 gtacacatcg tgaaacacga ctatgacctc atgctttcgg gtcactcaca cggaggacaa
     1141 gtcacctggc ccatcgtcgg ttctctcgca atacctcagc gcagtaaaaa atattctcgc
     1201 ggtctgcacc agatcaatca ccgaaaacgc atttacatca acaggggcct gggacacaca
     1261 tttcgtctgc gcatcaattc tcagccagag attacccttt tcgaaataat ataatcaaat
     1321 aaaacaaata tattcaacgt gttaacctga ctatcctgtc gtc
//
LOCUS       DATDUN010000098         8160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_503062, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000098 DATDUN010000000
VERSION     DATDUN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503062"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VJC18_01400"
     CDS             complement(<1..695)
                     /locus_tag="VJC18_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44062.1"
                     /translation="MNTADILSQFQTAYYSPARCDEHQWQNTQEGLTTQADSIREKEL
                     VALLNQSLSDFAISPEEQDQLRKKFFLSTTLRPKLWQGLLTSTPATWTDWAIGYFNAK
                     TRSTHRLDAEDTALVRTLFSIPSVQLSADALALLTTTLYSTESDLVQSAWNHLASLGP
                     VAHEYAKDLVLASLLVDEQIVVPKRDVFGITAQEETDILIDFLETVTQANLQENSSLI
                     KRLGTTGEAPSQQV"
     gene            1151..1942
                     /locus_tag="VJC18_01405"
     CDS             1151..1942
                     /locus_tag="VJC18_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44063.1"
                     /translation="MTAVGLFSQSIPLNFGGRVAPTAVALPLGAFQLQAPVEDAPDDV
                     DIGDYMEPEICDSDSAFTISGSEFKEGTAFVAPQPMAQTEAQTAVPSGDQQARPVAVA
                     LEMPKLPKPYRVTAPSQPLFMNEIDQLVETQLIEGPDGVVDIHFSFTRAVEPKSFVKI
                     VEGLLYYATVKPGITLESDRRDSLQWQVKSLDDGSFSSNLSLKFLEERSKRSFWGLLD
                     RGQGRIVSCVLSVRKSDSAVRVPAHPEDRKILLSIARMMGLVKLP"
     gene            complement(1968..3413)
                     /locus_tag="VJC18_01410"
     CDS             complement(1968..3413)
                     /locus_tag="VJC18_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44064.1"
                     /translation="MAVDPTRIPPATDRTSPLLEDNDKGVANTHDTGDITPTSTDTTE
                     KTRPIGDSEYVGDAPPSLFEPDDLGPLYEVSEQQYALIFSLSNPDTGLSPIQIFQRAY
                     MDFNPEFDPDAGNKPVALDHDERDLGVTNHYGIVSDGFVGPETAEALEDCLDDIQRDG
                     GFAISPRWEHILPQSLRDNMTEKGVVEMETTPSLPYSGLSTNLFASQARKSRPEDPPE
                     QESGNRPAQTSKTTGNTPPEQTTQKKAESPHKTAKTTVSEPVDPTKFKPTQQQALDIV
                     KNFVFSDFSNPGNISRALQVLTLEANPAIKEKFNKFEKAFLQYSAFEKTPPHLVTAYD
                     RFIEENYDLLYPASEFGSSFCDVSYTHQTYEWMSQLVSSVYHQRRVPEEYYPLIRELV
                     TLVETTEVTESSLEGLGIEPSETYTPYQQYLMYFETLWMDIVGRYQQELDQIPRETQL
                     ASVRERFGAQHMFGKWQDLRTKAEAAMDANK"
     gene            complement(3595..4785)
                     /locus_tag="VJC18_01415"
     CDS             complement(3595..4785)
                     /locus_tag="VJC18_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLD44065.1"
                     /translation="MSFCAIFLPTTPAQAKLLQNTEKKFFPVPVFETRPDEGQTYGLM
                     PVVLFSEKESQAISLILATIGQYNTVVHASGAVALYYYPHPVKNPEEVFDFYFEFAQK
                     VYRETTLHYSNPNLTEHIFVDGSFLWLETPFGRFYGYGPETSSVAESSFTSKNLKSRI
                     TFGYFVLPKLRVNLTEQIASTSLSAGIIPDTADTLASFAGTTGVVDNTSLLHSLSLTY
                     DTRPSGMNSQTGVLLEGTYFFAIPGLVSTDTLNGFSLQAIGLLPWKNKKMVSALRFFL
                     QDMFGKDIPFYMQSSLGGPNELRSYVPGRFTDTGKVILSAEHRFHVLSAKVFHVPLQL
                     HIDPFVEFGRVFSHVGDFGFDHWQPVVGIGLRGKVPPNVVARVDAALGREGYSVYTML
                     GFAF"
     gene            complement(5059..6402)
                     /locus_tag="VJC18_01420"
     CDS             complement(5059..6402)
                     /locus_tag="VJC18_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLD44066.1"
                     /translation="MKVPSRLPNVDAVFARILKTKPAAETTRLLAAIDVVDAKGRYLH
                     WEKLKHIPPVENLTSEEWWAFTKIKRRSLFQVLPQLTDAAGKPFQLLITPAIIQTLFF
                     LEHPFDQLKGSLAHKKKTAQQSLIDEAISSSQLEGASTTRHIAKEMLAQNRPPKNLSE
                     QMIHNNYQAMLFIQDMKEEDLSVGIIKELQRILTRDTLSHKKKSGQFRTSEDRVDVVD
                     MFGEVLHEPPEAKRLSTRLNRLCAFANNNNNDNNSRFMHSIIKGIILHFMLAYEHPFV
                     DGNGRTARALFYWHCAKHHQALMQYLSISQIIRTAPVQYGKAFLHTETDDNDLTYFVL
                     QQLEVITKALHLLRENLNSLLQESLRTKSLASRTALLITSLNQRQVALVQHALRHPCY
                     VYQISEHQTLHGIVYETARTDLLELSDRFKYLKKMKHGKKFVFTAIKSLKDKLKG"
     gene            complement(6469..6687)
                     /locus_tag="VJC18_01425"
     CDS             complement(6469..6687)
                     /locus_tag="VJC18_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44067.1"
                     /translation="MKFFLICILEQSQKERQMYFYRNSNGENLIFVCLRASGPKHTGT
                     GQIELWDTRPNSKISLDVWGKYYYTTNY"
     gene            6791..7177
                     /locus_tag="VJC18_01430"
     CDS             6791..7177
                     /locus_tag="VJC18_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44068.1"
                     /translation="MKNHIHKTGRLMLSVLVLMALAVLGQEQTVHAEEILDCPNCLIM
                     LPLAEFYDYPVTSLPNEKEVGEDRVPPKKCPNAFEIMPLSEFYDYPVTNVPNEEETGE
                     DSVPPKKCPNGYIMVPLDEFYDYPVT"
     gene            7341..>8160
                     /locus_tag="VJC18_01435"
     CDS             7341..>8160
                     /locus_tag="VJC18_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HLD44069.1"
                     /translation="MLIKIAHSPDSDDAFMFWALKHGLVASAQYQFQIERHDIEQLNQ
                     LAKDEVYDITALSLHAYAHVTDRYVLTSSGASFAEKDYGPLVVAKKNISPDQLFKHVV
                     AVPGELTTALLLLKIFEPRLTYLVMPFDQIIPAVLGGRVDAGLIIHEGQLQYQKMGLT
                     RILSLIDVWHGLAGDLPLPLGGSAIKRSLGEKIISDLSNLQKQSIQFALNHPEEARDY
                     ARGFKRDMTTDEADQYLRWYANERTLEMGEEGQRAIQLLYELARERGLLMAQSSK"
BASE COUNT         1931 a         1794 c         2172 g         2263 t
ORIGIN      
        1 acttgttgtg acggggcttc accggtcgtg cccaatcgct tgatgagtga gctgttttct
       61 tgcaggttgg cttgagtgac ggtctctaaa aaatcaatca gaatatctgt ttcttcttgc
      121 gcagtgattc caaagacgtc ccgtttgggg acaacgattt gttcatcaac caagagtgag
      181 gccaaaacga ggtcttttgc gtattcatgg gccacgggac ccagtgaagc taggtggttc
      241 caggcacttt gaaccagatc actctctgtg gaataaaggg ttgtggttag caaagcgagc
      301 gcatcagccg agagctggac agaagggatt gagaacagcg tgcgcaccag agcagtgtct
      361 tctgcgtcga gtctgtgagt gcttcttgtt ttggcattga aataaccgat ggcccaatca
      421 gtccacgtgg ccggggttga ggtcagcagg ccttgccaca attttggtct caacgtggtg
      481 gagagaaaga atttttttct gagttgatcc tgttcctctg gagagatggc gaagtcgctc
      541 aaagactgat ttagtagggc aacaagttcc ttttctctta tggaatctgc ttgtgtggtc
      601 agtccctctt gcgtgttctg ccactgatgt tcatcacatc tggcgggtga ataataggcc
      661 gtttgaaatt gtgacaggat gtctgcggta ttcatgaaat aagatagaag caagggttgt
      721 gccaattcta attaattttt tttggattga tatcattaga taaaaatgag ggagtcatga
      781 tcatgttttt tctatgtgtg caaatgtatg gttgttctgt ccaaaaaatc acacatttgc
      841 gcaatatttt ggacacccat aacgcataac tcattaccca ttacataaaa ttccactgaa
      901 accggtcact caatggcgcg gtttgacttt tcgggtcctg ctccgggtcg ctattttttg
      961 ccgctcttcg caagcagggc ttgctccgca ggccgcgtca aaataatagc ttcccgtatc
     1021 accccaaaag tcaaactgcg cctacgattg acgcattact tcccactgaa attccctctt
     1081 gtgatttgca tagaaagtgt agtaaaaggc ggacccgttt tttgggattt attgaaaagg
     1141 aaggaatttt atgacagcag tgggtttatt cagccagtcg ataccgctta attttggagg
     1201 gagggttgct cctacagccg tggccttgcc attgggagca tttcaattac aagcgccagt
     1261 cgaagacgct ccggatgatg ttgatattgg tgattatatg gagcctgaaa tatgtgatag
     1321 tgatagtgcc tttaccattt cgggtagcga attcaaggaa gggaccgcgt ttgttgctcc
     1381 ccaacccatg gcacagacag aggctcaaac ggcagtccct tcaggtgatc agcaggcgag
     1441 gcccgttgcg gttgccttgg agatgcccaa gttgcccaag ccgtaccggg ttacagcacc
     1501 gtcccaacct ttatttatga acgagattga ccagctggtt gagacccaat tgattgaggg
     1561 tcccgatggt gttgtagaca ttcattttag ttttaccaga gcagttgagc caaagagctt
     1621 tgtcaaaatt gtggaaggtt tgttgtatta tgccacggtc aagccaggga ttaccctgga
     1681 atccgatagg cgggattccc tgcaatggca ggtgaagtcg ctcgatgacg ggagtttttc
     1741 cagcaacctc tccctaaaat ttttggagga gagatcaaaa cgcagttttt ggggtttgct
     1801 ggatcgtggg cagggccgca tcgtttcctg tgtgttgtcc gtccgtaaat cagattcagc
     1861 tgttcgtgtg cccgctcatc ccgaggatag aaaaatcttg ctctccatcg cccggatgat
     1921 ggggttggtg aagttgccgt aaaggggtgc gggtttgatt tttaattcta tttgttggcg
     1981 tccattgcgg cttcggcttt ggtcctgagg tcttgccatt tgccgaacat atgctgagcc
     2041 ccaaatcgtt cgcgcacgct ggccaattgg gtttctcggg ggatttgatc aagctcctgt
     2101 tgatacctgc cgacgatatc catccatagg gtttcgaaat acatcaggta ctgctgatag
     2161 ggtgtgtagg tttcggaagg ttctatcccc aaaccttcca gcgaactttc tgtaacctct
     2221 gttgtttcga ctaatgtgac cagttccctg attagaggat aatattcttc ggggaccctg
     2281 cgttgatggt acacagacga aaccaattgc gacatccatt catatgtttg atgggtataa
     2341 ctcacatcgc agaaacttga accaaattca gaggcagggt aaagcaggtc atagttttct
     2401 tcaatgaacc ggtcataagc tgttaccaga tgaggagggg ttttttcgaa agctgaatac
     2461 tgcaggaagg ccttctcaaa tttgttgaac ttttccttga ttgctgggtt cgcttcgagg
     2521 gtcagcactt gcagtgcgcg tgaaatattg cccgggttgg aaaaatcgct gaagacaaaa
     2581 tttttcacga tatcgagggc ctgttgttgt gtgggtttga acttggttgg gtcgactggt
     2641 tcgctgacag tcgtctttgc agtcttgtgg ggtgactccg ctttcttttg agtggtttgc
     2701 tctggtgggg tgtttccagt tgtttttgag gtctgagctg gtctgtttcc tgactcttgt
     2761 tcgggaggat cctcaggacg actctttcta gcctgcgaag cgaacaggtt agttgacaaa
     2821 ccgctgtagg gcagtgaggg tgtcgtctcc atttcgacta cccccttctc ggtcatatta
     2881 tctcttaggc tttgcggcaa aatgtgttcc catcgaggcg aaatcgcgaa ccctccgtcc
     2941 cgctggatat catcgagaca gtcttccagt gcctccgccg tttctgggcc aacaaaaccg
     3001 tcgctgacga tgccgtagtg atttgtgacc ccgaggtccc gttcgtcatg atctaaagca
     3061 accggtttgt tgccagcgtc tggatcaaat tccggattga aatccatgta cgcgcgttga
     3121 aaaatttgga taggagagag gccggtatcg ggattggaga ggctgaaaat cagggcatac
     3181 tgctgttcac tgacttcata gagcggacca aggtcatcgg gctcaaagag agaaggaggg
     3241 gcatccccta cgtattcact gtcaccaatg ggtcttgttt tctcggtggt atcggttgag
     3301 gttggggtaa tatctcccgt atcgtgcgta tttgctacgc ctttgtcgtt gtcctctaac
     3361 aggggggagg tgcgatctgt agcgggagga attcgggttg ggtcaacagc catgaagctc
     3421 ctttgatcgc cattgctgat cattcagcgc aggagacctt ggatacacag ctccattgta
     3481 taccaatgcc tgattgcagg aaataacatt tcgcgcttac agtgaggtga taggacagga
     3541 cctgacaacg caggacaggg ttaacaatgt gatgaggtta gcccccagcg attttcaaaa
     3601 cgcgaagccc agcatcgtgt agacgctgta gccctctctg ccgagggcgg catcaacgcg
     3661 cgcgaccacg ttcgggggga ccttgccccg caaaccaatg cccacaacgg gttgccaatg
     3721 atcaaaacca aagtcgccca cgtgtgaaaa cacgcgtccg aattcgacaa atggatcgat
     3781 gtggagctga agggggacgt gaaacacctt ggccgacaac acatgaaatc tgtgttccgc
     3841 gctcaggatc accttgcccg tatcggtgaa tcgcccgggg acgtaagagc gcaattcgtt
     3901 cggccccccc aacgatgatt gcatataaaa ggggatatcc ttgccgaaca tgtcctgcaa
     3961 aaaaaatctc agggcggaga ccattttttt gtttttccag ggcaggagac caattgcctg
     4021 caaagaaaat ccgttgaggg tgtctgtgga aaccagaccg gggatcgcaa aaaaatacgt
     4081 gccttcaagc aggacacccg tttgactgtt catgccgctg ggtcgtgtat cataagtcag
     4141 ggaaagggag tgaagcaggg aggtgttgtc aacgacccct gtcgtgccgg caaacgaggc
     4201 gagtgtatct gccgtgtccg gaatgatgcc tgcggaaagg ctcgtggagg caatttgttc
     4261 ggtcaggttc acgcggagct tgggcaacac aaaatatccg aacgtgattc gcgatttcag
     4321 gttcttcgag gtgaaactgc tttcggcaac agacgaggtt tctggaccat atccataaaa
     4381 tctgccgaac ggtgtttcca gccagagaaa actgccatcc acaaagatgt gttccgtcag
     4441 gttgggattg ctgtagtgga gtgtggtctc gcggtagacc ttctgggcga attcaaaata
     4501 aaaatcaaag acctcttccg gattttttac gggatgcgga taataataca atgccaccgc
     4561 gccgctggcg tgaacaactg tgttgtattg cccgatggtg gccaggatga gggagatggc
     4621 ttgggactct ttttcggaaa aaaggacaac aggcatgagt ccgtacgttt ggccttcgtc
     4681 gggacgggtt tcgaacacgg ggaccggaaa aaactttttc tcggtattct gcagtaactt
     4741 cgcttgtgcg ggggttgttg gtaaaaagat tgcgcaaaag ctcacggcga gcagaagaag
     4801 gcaacaagaa aaccccgaac gggaatgatg acatgtgggt ggtgattgtt gattgtgtgg
     4861 tcgaatgagg gtctccggga aaacgggtgg tctgaccctc cctaacacag tttgtttccc
     4921 cagtccattt ttgcaagcga ccatatcatt cgtagagact ttgtatctta atacagcaca
     4981 tgataggatg ttttttcgat tcttgttttg acggttcttg tgcaaccatg ccgggttgta
     5041 ccagttgagg acttcacgtt aacccttcaa tttatctttc aggcttttaa tggcggtgaa
     5101 gacgaatttt tttccgtgtt tcattttttt taaatatttg aaacgatcag acaattccaa
     5161 caggtcggta cgcgctgtct cgtaaacgat gccgtgcaaa gtctggtgct cagagatctg
     5221 atacacgtaa cagggatgcc gcagggcgtg ttgcaccaat gcgacctgtc gctggtttaa
     5281 agaggttatc agtaatgccg tacgggatgc cagagatttt gttcgcaggc tttcctgcaa
     5341 caggctattc aaattctctc tcagcagatg aagtgccttc gtaatgactt caagctgttg
     5401 aagaacgaaa taggtcagat cgttgtcgtc ggtttcggta tgcaaaaaag cctttccgta
     5461 ttgcaccggg gctgtccgaa tgatttgcga tatggaaaga tattgcatta gcgcctggtg
     5521 gtgcttggcg cagtgccaat aaaatagggc cctggcagtc ctgccgtttc cgtcaacgaa
     5581 ggggtgttca tatgccagca tgaaatgcag aatgattcct ttgatgatgc tgtgcatgaa
     5641 acgagagtta ttatcattat tgttattgtt cgcgaaggcg cacaaacgat tcagacgggt
     5701 tgataatctc ttggcttcag gcggctcgtg caggacttcg ccaaacatat cgaccacatc
     5761 aacgcgatct tcagacgtgc gaaactggcc cgattttttt ttatgactta gcgtatctct
     5821 ggtcagaatt cgttgcagtt ctttgatgat gccgacagac aggtcttcct ctttcatgtc
     5881 ctggatgaac agcatcgcct gatagttgtt gtggatcatc tgctcgctca gattttttgg
     5941 gggacggttt tgagccaaca tctctttggc gatgtgacgc gtggtggagg ctccttcgag
     6001 ctggctggaa ctgatggctt catcgatgag tgattgttgt gctgtctttt ttttgtgtgc
     6061 gagagagccc ttgagctgat cgaaggggtg ttctagaaaa aacagggttt gtatgatggc
     6121 aggcgtgatc agtagctgaa agggttttcc tgccgcgtcg gtcaactgcg gcaggacttg
     6181 aaacagactg cgtcttttga ttttggtaaa agcccaccat tcttcgctgg tgaggttttc
     6241 tacagggggt atgtgtttca gtttctccca gtggagataa cgacccttcg catccaccac
     6301 atcgatggcg gcgagaagtc gggttgtttc agcggcgggt ttcgttttga gaatgcgcgc
     6361 aaaaacggca tcgacgttcg gtaatcggct ggggactttc ataaatatgt actgttgttc
     6421 ggcaacagtg attgcttttt accaacacaa accagtttga tactaatatc aataattagt
     6481 agtataatag tatttgcccc agacatccag tgatattttt gagttgggtc gggtgtccca
     6541 caattctatt tgacctgtac cggtgtgttt tggtccgctg gcccgcaaac acacaaaaat
     6601 gagattctcg ccgttgctgt ttcgatagaa atacatttgt cgttctttct gactttgttc
     6661 caaaatacat attaaaaaaa atttcatgct atttcgtttg gttacgatgt tctttttgtg
     6721 cgtggcatcg agcttgcatt gttatgtggt gtgagtttga gggtcgttaa gacaacgaga
     6781 aggagatgtt atgaaaaatc atattcataa aacagggcgt ttgatgttat cggtactcgt
     6841 tttaatggca ttggctgtgt tgggccaaga acaaacagta cacgccgaag aaatattgga
     6901 ttgccccaac tgtctgatca tgctcccact tgccgaattc tatgattacc cggtcaccag
     6961 tcttcccaat gaaaaagaag tgggggaaga tagagtccct cccaaaaaat gtcccaacgc
     7021 gtttgagatt atgcccttgt ctgagtttta cgattacccg gtgactaatg ttcccaacga
     7081 agaagagacc ggagaagata gtgttcctcc caaaaagtgc cccaatggct acatcatggt
     7141 tcctcttgat gaattctacg actacccggt gacgtaatga gaatcgtgaa tcgtggttcg
     7201 agaatcgtgg atcgaaattc aggttgccag acgacaactc cccctgcccc ctcttgttaa
     7261 gggggttctt agaggtggcc ttaattttcg tcgacctaat tctcttgact ttggtctttg
     7321 gtctttggtc tttggtctgc gtgttgatca aaatcgccca tagccctgat tccgatgacg
     7381 cgtttatgtt ctgggccctc aagcacggtc ttgtggcttc tgctcaatac caattccaga
     7441 ttgaacggca tgacattgaa cagctcaatc agctcgctaa agatgaggtc tacgacatca
     7501 cggcattgtc ccttcacgcc tatgcccacg tgaccgatcg ttatgtgttg acctccagtg
     7561 gggcctcatt tgccgaaaaa gattatgggc cgttggtggt ggcaaaaaaa aacatttctc
     7621 cggatcaact tttcaaacac gttgttgccg taccgggtga gttgaccacg gctttgctgt
     7681 tgctgaaaat attcgagccc cgattgactt atctggtcat gccgtttgat caaatcattc
     7741 cggctgtgct cggaggtcgc gttgacgcag gactcattat tcacgaagga cagttgcagt
     7801 atcaaaaaat gggactgacc cggatcttgt cgctcatcga tgtctggcat gggctggctg
     7861 gtgatttgcc attgcctttg ggcgggagtg ccatcaaaag gtccttgggt gaaaaaatca
     7921 tttctgattt gtcaaatctg caaaagcaaa gtatccagtt cgcactgaat caccccgagg
     7981 aggccagaga ttacgcgcga gggttcaaac gcgacatgac cactgacgag gccgatcagt
     8041 atttgcgctg gtacgccaat gagcgaacgc tggagatggg ggaggaaggg caaagggcca
     8101 ttcagttatt atatgagctg gcgagagaac ggggactgtt gatggctcaa agctcaaaga
//
LOCUS       DATDUN010000099         1201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_379028, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000099 DATDUN010000000
VERSION     DATDUN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379028"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(443..>1201)
                     /locus_tag="VJC18_01440"
     CDS             complement(443..>1201)
                     /locus_tag="VJC18_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 36 associated protein"
                     /protein_id="HLD44070.1"
                     /translation="RATFDYGSLLGSKANAEAKIDSLPQSWAWLSGAANKDRALQALE
                     SAWKYLVKEDEKLILLFTPPFDESNPSPGYIKGYPPGVRENGGQYTHAALWFAMALAR
                     SGNGDRAAELMRFINPIERTRTPEAVWLYNLEPYVVAADVCHLKGHVGMGGWSWYTGS
                     AAWMYRAWVEECLGLKICGSTLRIDPVIPKKWDGFQMSYHHGEALYEIRVENPDHCAK
                     GVLWTELDGQRVSDHQIALDKALVKHQVVVRMGR"
BASE COUNT          362 a          307 c          238 g          294 t
ORIGIN      
        1 gttaacaact ctgaaaaaat ctgagaacaa tttttgtgac gagcaccgcc ccgttaaatg
       61 tacgtggttg tccattaaac aataggaaaa gaaaatgaca tcatagatgg ttttgtattt
      121 gagcaaaagg tcataatata gggcctttgc aaattcagaa ttcaaaagcc aatctttgtt
      181 atgacattgc catgtgacat gaaaaacaga atcttcgaaa accaataaat ttctgggata
      241 atttgccatg ctctatgcat tagctaaaag tgtgccaaca tgaaaaaaaa attcaactaa
      301 tgatattggg aggttaactt aacacttaaa aaaaactccg tcccggagca acaaaaacgt
      361 ctcagatatg ggactgtgag tggaacaccg tcatgactat tttgatgact attttgagtg
      421 gaactccgtc atgactatct tattatcttc ccatcctcac caccacctga tgtttcacca
      481 gagccttatc cagagcgatc tgatgatcgc tcactcgttg accgtccaac tccgtccaca
      541 acacaccctt tgcacaatga tcgggattct caacacggat ctcgtaaaga gcctcaccat
      601 gatgataact catttgaaaa ccatcccatt tcttggggat gacaggatca atccgcagtg
      661 tactgccaca tatctttagt cccaaacact cttccaccca ggcacgatac atccacgccg
      721 cagaacctgt gtaccaagac cagccaccca tgccaacatg acccttgagg tggcacacat
      781 cagcggccac cacataaggt tcaaggttat aaagccagac agcttcggga gtgcgggtac
      841 gctcaatggg attgataaac cgcatcagct ctgccgctcg atctccgttt ccgctcctgg
      901 ccaatgccat agcaaaccat aaagccgcat gcgtgtactg accaccattt tcccgaaccc
      961 ccggaggata ccctttgatg taaccaggtg agggattcga ttcatcaaag ggaggagtga
     1021 aaagaagaat gagtttttca tcttctttca ccagatattt ccaggcagat tccaaagcct
     1081 gcagggcacg atctttgttc gccgctcccg aaagccaggc ccacgactga ggcaaagaat
     1141 cgattttcgc ttctgcgttc gctttggatc ccaaaagcga accataatca aacgtggcac
     1201 g
//
LOCUS       DATDUN010000100         1548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_543887, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000100 DATDUN010000000
VERSION     DATDUN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543887"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VJC18_01445"
     CDS             <1..1055
                     /locus_tag="VJC18_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44071.1"
                     /translation="RPEDGVVNLALGLGKTIVDGSLCWTYCPEMPRVPPPTNSVADLV
                     RQTQNDFWAINMRPSTHYDPTKETEHMCLFGLKDAEYDNTLTQVASTYRPESDSLMVG
                     TYQAGPRVITFAPILDAETLPLNRLVKNTLAACEKSLQNAVEIEFALSHDRENNRYQF
                     GFLQVRPMVVCHTTVDLTAEISQPERCVVRSNKVLGNGLVEGLTHIVYVKPLAFDPAQ
                     TRLIASEIAAINQTLQQQQQPYVLIGFGRWGSSDPWLGIPVRWSDICQAKVIVEATLS
                     NMNVDLSQGSHFFHNINGFQVSYFTVSHNDPGIDWNWLDHCQVVSETKYVRHICVSAP
                     LTVKVDGRSQQGVIHK"
     gene            1052..>1548
                     /locus_tag="VJC18_01450"
     CDS             1052..>1548
                     /locus_tag="VJC18_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44072.1"
                     /translation="MTQQPQSSHDDLLASLHERAKELNCLYLVEEIISDKNNSQDEIF
                     AKIVEAIPLGFSYPEACQTKIICQHQEYKTQQYETPCATLTSEIIVADQSIGFLEVSY
                     HTDKPSRDEGPFLKEEKKLINTITERLSQSILHKQMESLFKKRPLTDSNFSLHHDWRS
                     IVELLA"
BASE COUNT          430 a          417 c          347 g          354 t
ORIGIN      
        1 aaaggcccga agatggcgtg gtgaatctgg ccctggggct gggcaaaacc attgtcgatg
       61 gcagtctgtg ctggacatac tgcccagaaa tgccgcgcgt cccgcctccc acaaacagtg
      121 tggcagacct cgtgcgacaa acgcaaaatg atttttgggc catcaacatg cggccctcaa
      181 cacactatga ccccaccaaa gaaaccgaac acatgtgtct ctttggactc aaagacgctg
      241 aatacgacaa cacactgaca caagtcgcgt ccacgtacag gccagaatca gattccctga
      301 tggtgggcac ttatcaggca ggaccacgcg tcatcacatt cgctcccatt ctcgacgctg
      361 aaaccctgcc gttaaacagg cttgtcaaaa acacgctggc cgcctgtgaa aaatctctgc
      421 aaaatgccgt ggaaattgaa tttgcactct cccatgatag agaaaacaac cgatatcaat
      481 tcggtttttt gcaggtacgc cccatggtgg tctgtcatac gactgtggac ttgacagcag
      541 aaatcagcca acccgaacgt tgtgtggtac gctcaaacaa agtcttgggc aacggcctcg
      601 ttgagggact aacccacatt gtttatgtca agccgctggc ctttgacccc gcccaaaccc
      661 gtctgatcgc ctcagagatc gccgccatca accaaacact gcaacaacaa cagcaaccgt
      721 acgtgctgat tggttttggt cgatggggca gttcggatcc ttggttgggc attccagttc
      781 gctggtctga tatttgtcag gcaaaagtta ttgttgaagc aacactgtcc aacatgaatg
      841 tcgacctcag ccagggctca catttttttc acaacatcaa cgggtttcag gtcagctact
      901 ttacagtttc acacaatgat ccgggcatcg actggaactg gctggaccac tgccaggttg
      961 tctctgaaac aaaatatgtc aggcacattt gcgtctctgc accgttgaca gtcaaggtcg
     1021 atggcagatc acaacaggga gtgattcaca aatgacacag cagccacaaa gcagtcacga
     1081 tgatctgctt gcgtcactac acgaaagagc caaagagttg aattgtttgt atctggttga
     1141 ggagattatt tccgacaaga acaattcaca ggatgaaata tttgccaaaa tcgttgaggc
     1201 catccctttg ggtttcagct atccggaagc ttgtcaaacc aagataatct gtcaacacca
     1261 ggaatataaa actcagcagt atgagacccc ctgtgccacg ctcacctctg aaatcattgt
     1321 cgctgatcaa tccataggtt ttttggaagt atcctatcat accgacaaac cctcgcgcga
     1381 tgaggggcct tttctcaaag aagaaaaaaa gctcattaac accatcacag aaaggctcag
     1441 ccagtccatc ctgcacaaac agatggagag cttgttcaaa aagcgtcccc tgaccgattc
     1501 caacttctcc ttgcatcatg actggaggtc catcgttgaa ctcctggc
//
LOCUS       DATDUN010000101         2638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_5439, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000101 DATDUN010000000
VERSION     DATDUN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5439"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VJC18_01455"
     CDS             <1..869
                     /locus_tag="VJC18_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44073.1"
                     /translation="KAKLQRMARQVGAIRQQTDAKSQKNTTELMRAERSDLAKQQLAA
                     KLSQSGSQSKFEQILQQVLAGNKTATQELAEGLKARFLIKTDAEWEAFFKNVLALDST
                     EVQSQAQLSKMIEALFRGLYKKPGTDQTLLIADLAMNENGEVSENKFSKLALNDPALQ
                     DLFGQLKPGDVIPPALLQKLGQEFEFIKLVNLLQLVVLSEAQKADILKAYRQQHSPEM
                     QRRLEQALLGQREDDAKRERDNQAPFVYAGHVFEKPEQHKGNPKLFMSLFYGVAGITA
                     ALILYILARHLF"
     gene            complement(898..2277)
                     /locus_tag="VJC18_01460"
     CDS             complement(898..2277)
                     /locus_tag="VJC18_01460"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="catalyzes the formation of D-fructose 6-phosphate
                     from D-glucose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HLD44074.1"
                     /translation="MNSRHKITLDLNNLFRPAIASGLPSLSGLEAPFSSAHQAITALK
                     QTGQLPFSSLPEDSITLENVLTAAQQYRGVQNVVVLGIGGSALGTTSIYHALYGAQAN
                     LMRGRDADTPRLFVVDHVEPQTLTELFDLIKNDRNIFIVISKSGNTSETLAQYLLVKK
                     YFGPDSLKNMFFITDPHKGFLHDLVVKHNYPSLPVPAGVGGRFSIFTPVGLFPLALCG
                     VDIKVLLEGARHMELECRQSLLAQNPAGLIALATHSLQEQRLLTQVVMLPYADRLRYF
                     SDWFAQLWGESLGKRFTVGGQEVFTGSTPIKSVGVTDQHSQLQLYLEGPKDKIIYFVE
                     VGHFAQPGRLSDDRHDNDQIDFLAGKSLETLMASEKAATEECLRENNRPNATIKLSEI
                     NEFQLGQLYQLFMNVIPYLGTLLNINAFDQPAVERIKQFTFGLLDRKGFEAYKDKLKT
                     APKNKELLF"
BASE COUNT          642 a          620 c          756 g          620 t
ORIGIN      
        1 ccaaggccaa actgcaacga atggcgcggc aggtgggtgc tattcgccag cagacagatg
       61 ccaaatcaca aaagaacacc acggagttga tgagggccga acggagtgat ctggccaagc
      121 aacagctggc cgcgaaattg agtcaatctg gttcgcaaag taaattcgag caaatattgc
      181 aacaggtttt ggcgggcaac aaaacagcaa cgcaagaatt ggctgaggga ttgaaggccc
      241 gttttttgat caaaaccgat gcggaatggg aagccttctt caagaatgtt ttggcgctgg
      301 acagtaccga ggtccagtct caggcgcaac tttcaaaaat gatagaggcc ctttttcgcg
      361 gtttgtataa aaaaccggga acagaccaga cgctcttgat tgcggacctg gcgatgaacg
      421 aaaatggaga agtgagcgaa aataaatttt ccaaattggc ccttaacgat ccagccttgc
      481 aagacctgtt tggccaactc aaaccgggcg atgtgatccc gccggccctt ttgcagaaac
      541 tggggcagga gtttgaattc atcaaattgg tgaacctgtt gcagttggtt gttttgagtg
      601 aggcgcagaa ggcagacatt cttaaggcct atcgccaaca gcactcaccc gaaatgcaac
      661 ggcgtctgga gcaggcttta ttgggacagc gggaagacga tgcgaaaagg gaacgggaca
      721 atcaggctcc ttttgtttat gcggggcatg tgtttgaaaa accggagcag cacaaaggga
      781 atcccaaact tttcatgtct ctgttttatg gtgttgcggg gatcacggcc gcgcttatcc
      841 tttatattct tgcccgacac ctattttaat cccccctaaa aatgctggcg ccaaggttca
      901 gaataacagt tctttattct tgggggctgt tttgagtttg tctttgtaag cttcaaagcc
      961 cttcctgtcc aagagtccaa aagtgaactg tttgatgcgt tcaacggcag gttggtcgaa
     1021 ggcgttgatg ttgagcaggg tgcccaggta ggggatgacg ttcatgaaga gctggtacaa
     1081 ttgacccaac tggaattcgt tgatctcact gagtttgatg gtggcgttgg gtcggttgtt
     1141 ctcgcgcagg cattcttcgg tggcggcttt ttcgctggcc atcagggttt ccagagattt
     1201 gcccgccaga aaatcgattt gatcgttgtc atgccggtcg tccgacagtc ttcccggttg
     1261 cgcgaaatgc cccacctcca cgaaataaat gatcttgtct tttggtccct caaggtacag
     1321 ttgtaattgc gagtgttgat cggtgacccc gactgacttg atgggcgtgg aacctgtgaa
     1381 aacttcctgc ccgccaaccg tgaagcgctt gcccaaagat tctccccaga gttgggcaaa
     1441 ccaatccgaa aaataacgca gcctgtcggc gtagggcagc atcaccacct gggtcagcag
     1501 tcgttgctct tgtagggagt gcgtggccaa agcgatgagt cccgcgggat tttgggccag
     1561 cagagactgg cggcattcca actccatgtg cctggccccc tccaaaagaa ctttgatgtc
     1621 gacaccacaa agggccagag ggaaaaggcc tacaggggtg aagatcgaaa aacgcccgcc
     1681 cacaccagcg gggacaggca gggagggata attgtgtttg acgaccaggt cgtgcaaaaa
     1741 acccttgtgg ggatccgtga tgaaaaacat gtttttcaaa gagtccggac cgaaatattt
     1801 tttgaccaac aggtactgag ccagggtttc ggaggtgttg cccgatttgg aaatgacaat
     1861 gaaaatgttt cgatcgtttt tgatgaggtc gaagagttcc gtcagggttt ggggttccac
     1921 atggtcgacg acaaacaggc ggggcgtatc ggcatcgcgg ccacgcatga ggttggcctg
     1981 ggcgccatac agggcgtggt agatcgatgt cgttcccagg gcagaaccgc caatgcccag
     2041 caccacgacg ttttgcacgc cgcggtattg ttgggccgcg gtgagtacgt tttcgagcgt
     2101 gatggaatcc tcggggagag aggaaaaagg aagctgtccc gtttgtttga gtgccgtgat
     2161 ggcctgatgt gcgctggaaa agggggcctc aagccccgac agtgagggga gaccactggc
     2221 gatggcagga cggaacaggt tatttaaatc cagagtgatt ttgtggcgtg agttcatggg
     2281 gggacgatat attttttgca ttggtttgac gagtgaaaat ttattcacta ggtgcctatt
     2341 cgaaaacctt ccgagagtca cttttttggg tcctgccccg gctcgctgtt ttttggcgct
     2401 ctgggctcgc aagctcgccc agcggcctgc tggcctgttc gcgcaaaaac agctcaccgt
     2461 gtcacccaaa aaagactgct tcgcgggagg ttttcgaata tgaggttcta ggtaatctag
     2521 agaatcaact tctatgctcc caaagcctcc ccaaaacaaa cgcattctgg ccctcgatgt
     2581 ggggaccagg acctgtggcg tggccatgac agaccaaatg gccacgatct gtgaaccc
//
LOCUS       DATDUN010000102         1742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_379108, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000102 DATDUN010000000
VERSION     DATDUN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379108"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VJC18_01465"
     CDS             complement(<1..409)
                     /locus_tag="VJC18_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLD44075.1"
                     /translation="MKTLVIGATGIIGNHIVRALLKEGHEVIGLSRGVMPSVNLDGLS
                     VTRLKADLLDVKALKEAFKGVDWVFQAAGYYPRDAFHNKQHVAQALAGVRSVIAAALD
                     AGIERLVYTSSLTTIGQASEGGLADESLSYDLLG"
     gene            complement(406..1176)
                     /locus_tag="VJC18_01470"
     CDS             complement(406..1176)
                     /locus_tag="VJC18_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HLD44076.1"
                     /translation="MKNVTTNITMGQVFFILLTVLFSSVMFFYLGAKFGPRMLQLNTA
                     YRGVDEPFFPDEAQIKEVQEILKNNKHKFVFFDELQSNQPITQFPKDVQVTLPVAEKT
                     ELAANPEDSTLEAGQALADTGLVKEEVSPKIAQKEPPAEKKETAPIQVGVSEPVLPPE
                     ATDLTVLPEAGLTPQSEFAYSLQLGSYSSQSKAQQALDAWKKRGYSVRLIKTTNAGKG
                     LWYRLSLGEYVSQDEALVAQKKIMSAFRQNGRVIKYKK"
     gene            complement(1173..>1742)
                     /gene="argS"
                     /locus_tag="VJC18_01475"
     CDS             complement(1173..>1742)
                     /gene="argS"
                     /locus_tag="VJC18_01475"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HLD44077.1"
                     /translation="ARAMFENDRGESHNDRVRAFSEEKKALIAERVGLSAIKYADLSH
                     NLASDYVFNWDKMLAMEGNTGPYMLYAYARIMSIGRKGGVDFNRLSFDGKLLLLQAKE
                     IELAKELCRFGDVVREVALQLRPNLLTDYLYTLSKTFSGFYDKNTGVSVLEAETEELR
                     NSRLCLCGLTAKTLKQGLYLLGIEVVEEM"
BASE COUNT          415 a          513 c          391 g          423 t
ORIGIN      
        1 tgcccagcaa gtcgtaagac aacgattcat ccgccaaacc tccctcggag gcctgtccaa
       61 tagtcgtcag agaacttgtg tacaccagac gctcgatccc cgcatccaga gcggcggcga
      121 taacactgcg cacacctgcc agggcctggg ccacatgttg tttattatga aaggcgtctc
      181 gtggataata gcccgccgcc tgaaagaccc aatccacgcc tttgaacgcc tctttcagag
      241 ccttcacatc caacaggtcg gccttgagcc tggtcacgga caaaccatcg agattgactg
      301 agggcatcac accacggcta aggccaatca cctcatgccc ttccttaagc agggcccgca
      361 caatatgatt gccaatgatt ccggtggcac cgatgacaag tgttttcatt ttttgtactt
      421 gataactcgg ccgttctgcc gaaaggcaga catgattttc ttttgggcca ccagggcctc
      481 gtcctgactg acatactcac ccaggctcaa acgataccac agccccttgc cggcattggt
      541 cgttttgata agccgcacgc tgtatccccg ctttttccag gcgtccaacg cctgctgtgc
      601 cttgctctga ctggaataag aacccagctg caggctgtag gcaaactctg attgcggggt
      661 cagcccagct tccggcaaaa cagtcaagtc tgttgcctcg ggtggcaaaa ccggttcgga
      721 gactcccact tgaatgggtg cagtttcctt tttttctgcg ggaggttctt tttgtgcaat
      781 tttgggagag acctcttcct tgaccagacc agtatcggcc aaagcctggc ccgcttcgag
      841 cgtagagtct tctgggttgg cagccaattc agttttttct gcgactggta acgtgacctg
      901 aacatccttg gggaactgcg tgatgggctg gttcgattgc agttcatcaa aaaacacaaa
      961 cttatgtttg ttgttcttta ggatctcctg cacctctttg atctgcgcct catcgggaaa
     1021 gaagggttca tcgaccccgc gatacgccgt gttcagctga agcattctgg gtccaaactt
     1081 ggcccccaga taaaaaaaca tgactgacga aaacagcacc gtcagcaaaa taaaaaaaac
     1141 ctgccccatc gtgatgttgg ttgttacatt cttcacattt cctccaccac ttcaatccct
     1201 aacaaataga gtccttgttt cagtgtcttt gcggtcaacc cacaaaggca caaacgactg
     1261 tttctcagct cttccgtctc ggcctcgaga acagacaccc ctgtattctt gtcgtaaaaa
     1321 ccactgaaag tcttgctcaa ggtgtacaaa taatccgtca gcagattggg tctgagctgt
     1381 aaggccactt cccgcaccac atcgccaaaa cggcacaact ccttggccaa ctcgatttcc
     1441 ttagcctgca gcaaaaggag cttcccgtca aaggataacc tgttgaagtc aacacccccc
     1501 ttacggccaa tgctcatgat cctcgcataa gcatagagca tatacggacc cgtattgcct
     1561 tccatcgcca gcattttgtc ccagttgaac acataatcac tcgccagatt atgactcagg
     1621 tcagcgtact tgatggcaga caatcccaca cgctcggcga tcagggcctt tttttcctcc
     1681 gaaaaggcgc gcacgcggtc gttatgggac tccccacgat cattctcaaa catggcccgg
     1741 gc
//
LOCUS       DATDUN010000103         1423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_420306, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000103 DATDUN010000000
VERSION     DATDUN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420306"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..668)
                     /locus_tag="VJC18_01480"
     CDS             complement(270..668)
                     /locus_tag="VJC18_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44078.1"
                     /translation="MKRSLTLILSLLMIAVSGFWIGCAGEGDEATESASLYNEDHESL
                     QSKDHKGDFDFWSDDEEAEKITSSQVETLISTGRAQIGSANSNCKEWVRSTTVSALGN
                     NYCIPATASNQYSWVTDQVDCSSKVTNVAQ"
     gene            complement(688..927)
                     /locus_tag="VJC18_01485"
     CDS             complement(688..927)
                     /locus_tag="VJC18_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44079.1"
                     /translation="MNTPKTTIPILRTYSHKQALFLTFWAADGQQCPRGVRESVFVFE
                     RFSFLKLTVSYNIISITPDHSAFSLILISKQKENG"
     gene            complement(1118..>1423)
                     /locus_tag="VJC18_01490"
     CDS             complement(1118..>1423)
                     /locus_tag="VJC18_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44080.1"
                     /translation="YTYVFNEFKGCLILLGSTVGYPIPYSTQFTNPQKIERASTNVGY
                     AILPQADPNGLFSPPSTEATWTMMKNPKGDNTGVVYSEPKLITMPFEAAEESVCNNR"
BASE COUNT          347 a          329 c          330 g          417 t
ORIGIN      
        1 tcaagaaggg gtcggatgcg ttcaaactgt tctctggtga tatcgcttgg atatggttgt
       61 ctcatagaac aaaaatagag gatcttttat gatcctctat tttactgcat tttttgaaaa
      121 aagattatgg acaggctctt agagcttttg gcgtaaataa ccattacttg cggatcagca
      181 atgggtattt tcaccgtata cggacccttt gtcgctccag ctgccaatcc ttttgtgaca
      241 gttacacgtc cattagtata gctaccgatt cattgggcta cgttggtcac ttttgatgaa
      301 caatcaacct ggtcggtaac ccatgaatac tgattactgg ccgtagctgg aatgcaataa
      361 ttattaccca atgcagatac tgtagtgctc ctcacccatt ctttgcaatt cgagttcgca
      421 cttccaatct gtgctcgccc agtcgatatc agggtttcga cctgactgga ggtgattttt
      481 tctgcttcct catcatctga ccagaagtcg aagtcaccct tgtgatcttt gctttggagg
      541 gattcatgat cttcattgta aagggacgca gattccgtcg cctcgtctcc ctcgccggca
      601 cagccgatcc agaaaccaga gacggcaatc atcaacaggg aaaggatcaa ggtcaagcta
      661 cgcttcatgg cacttctcct tgttttttca accattctct ttttgttttg aaattaagat
      721 caaagaaaaa gcagaatgat caggagttat gctaatgatg ttgtagctga ctgtcaattt
      781 taaaaatgaa aatctttcaa aaacaaaaac actctccctc acacctctcg gacattgctg
      841 cccatctgct gcccaaaatg tgaggaagag tgcttgttta tgagaatagg tgcgaagaat
      901 gggaatcgtt gttttaggcg tattcattga gtaaactcac ctttttccca ttttcattgc
      961 attttgtttc ttgtcttcgg tcgggttcga tacccgtttt ccgctccaca aaaagacccc
     1021 agtaaaaact ggagtctttt ttgttgtgtt aaaacaaaac accccgccag accaacgagc
     1081 atccgaaggg atgttttgtt ggccggacgg ggtcgtatta cctgttgttg cagacggact
     1141 cttcagccgc ctcgaagggc atggtgatga gcttgggctc actgtacacc acaccggtgt
     1201 tgtctccctt gggatttttc atcatggtcc acgtcgcttc cgtggatggt ggggagaaca
     1261 ggccgtttgg atcggcctgg ggcaggatcg catagccgac gttggtactg gcccgctcga
     1321 ttttctgggg gttggtgaac tgggtcgaat acgggatcgg atacccgacc gtagacccca
     1381 ggaggatcag acacccctta aactcgttaa acacgtaggt gta
//
LOCUS       DATDUN010000104         1277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_295560, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000104 DATDUN010000000
VERSION     DATDUN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295560"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1277
                     /locus_tag="VJC18_01495"
     CDS             <1..>1277
                     /locus_tag="VJC18_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLD44081.1"
                     /translation="TRPETLFGDVAVAVHPDDDRYQHLIGKNVIVPLQGRAVPVIADE
                     YVDKAFGTGCLKITPAHDANDFEVGQRHGLEPINVMNEDATMNEVVPASYRGLDRYKC
                     REKAIEELKEQGLLIKEEPRMTPIGRSYRSKEPIEYRLSDQWFVKMKPLAEKVLALHN
                     GLNIVPQRWNKVYLDWMTNIRDWCISRQIWWGHRIPAWYCVGDQRPQTKDQSQTGDEH
                     TRDSCHPCESRDPNIVDLDSCLRRNDEPLYDPCPLGHKRSTIHDQRCCPPIVSETVPT
                     KCPHCGGTNLVQENDVLDTWFSSALWPMSTLGWPDKTKDFDRYFPTTTLVTGKDIIFF
                     WVARMNMMAAHFTGKLPYKNVFINPIVLDEKGETMSKSRGNGIDPLVIINGATLEELE
                     GPVREARPSNMKEILKRIEKNFPQGYEGVGADA"
BASE COUNT          334 a          312 c          354 g          277 t
ORIGIN      
        1 acgaggcccg aaacactgtt tggtgatgtc gctgtggcgg tgcatcccga cgacgatcgc
       61 tatcaacacc tcattggcaa aaatgtcatt gtccccctgc aggggcgcgc tgttcctgtg
      121 attgcggatg agtatgtgga caaagcgttt ggcacaggct gtctcaaaat tacaccggcg
      181 catgatgcca atgactttga agtggggcaa agacacggac tggaaccgat caatgtgatg
      241 aatgaagacg ctacgatgaa tgaggtggta ccggcgtcct atcgcgggct tgatcgctac
      301 aaatgccgcg aaaaggcgat tgaagaactc aaagagcagg ggttactcat caaggaagaa
      361 ccacgcatga caccgattgg tcggtcttat cgttccaaag agcccattga gtatcgcttg
      421 tcggatcagt ggtttgtcaa aatgaagcct ttggccgaaa aagttttggc ccttcacaac
      481 ggactcaaca tagttcctca gcgctggaac aaggtgtatc tggactggat gaccaacatt
      541 cgcgattggt gtatctcgcg ccagatctgg tgggggcaca ggattccggc gtggtattgt
      601 gtaggagacc aaagaccaca gaccaaagac caaagtcaaa caggggatga gcacacgagg
      661 gatagttgtc atccctgcga aagcagggat ccaaatattg ttgatctgga ttcctgcttg
      721 cgcaggaatg acgaaccact ttacgatcca tgccctctgg ggcacaagcg atccactatc
      781 cacgatcaac gctgttgtcc ccccatcgtt tccgaaactg tacccaccaa atgcccgcat
      841 tgcgggggca caaatttggt gcaggaaaat gatgttttgg acacgtggtt ctcctcggcc
      901 ctgtggccca tgtccacact gggttggcca gacaagacca aagatttcga tcgctatttt
      961 cccaccacca cactggtgac gggcaaggac atcattttct tttgggtggc ccgcatgaac
     1021 atgatggcgg cgcatttcac agggaagttg ccctacaaaa atgtgttcat caaccccatt
     1081 gtgctcgatg aaaaaggcga gaccatgagc aaatccagag gcaacgggat cgacccgctg
     1141 gttatcatca acggggccac cttggaggaa ctcgaagggc cggtgagaga ggcgcgtcct
     1201 tcgaacatga aggaaatatt gaagaggatc gaaaagaatt ttccacaggg ttacgaaggg
     1261 gtgggggccg atgcctt
//
LOCUS       DATDUN010000105         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_503231, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000105 DATDUN010000000
VERSION     DATDUN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503231"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VJC18_01500"
     CDS             complement(<1..892)
                     /locus_tag="VJC18_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLD44082.1"
                     /translation="MFEEYSKQLNLCKTCPSLCQSACPVFINDGNKTHSPQGLMQLVN
                     NVRHKQLPFDEQIASEAYHCLECRACTQACEHHVDVPQILRQVRTEAIRQELAPQPIN
                     TFIERFHKHNNPFARDLAGKLRSLLPARYFNANASAIYFSSCTTIAKCPEVIRDTFAL
                     FNKLKIDFVNLYADPIHCCGYPLLCCGAEYEFVDLAEINFHSLKKFKTIITNCPACAH
                     TLKHTYAKYAFNLEAQVITISDFLKPYLKPLRAALKKNIRTKTIFHDSCYATRYLDDP
                     DTSRDIITQATGHAPTRFYDH"
     gene            complement(894..1724)
                     /locus_tag="VJC18_01505"
     CDS             complement(894..1724)
                     /locus_tag="VJC18_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44083.1"
                     /translation="MFQLNKKSLLVTVSPDAFLPKIEHQLALEGCWLGYAPLGDQGQP
                     LRFHLDHNTPNLYHFKHGALSQIVSSLLCELPDGSVVHLKNAPRAATGPDIDNLFIGS
                     GSQLGQASLVTLRVMTQPEETTHGLLLMNKPEDGARFVRHLVGSFFDPLFFRQLDTKK
                     QPELTKRLKIKKTTCGVLLFGLSGLGEIVGIQKELINDWCAFEGLGIKWLQTKTERLI
                     VPQYIYAPSSGEDIKQQYHDMIWPSQKTATQAKTSQALLSRLFRQLNKSGNRLKGQEE
                     "
     gene            complement(1727..>2045)
                     /locus_tag="VJC18_01510"
     CDS             complement(1727..>2045)
                     /locus_tag="VJC18_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLD44084.1"
                     /translation="VLKKLAPHCLVLTHITHTYPDGASIYFTFVAPLKGSKNSVKLYD
                     EIWNSALDAVQEGGGVISHHNGIGRLKIRHAHKEWRDALPVLEHMKQFFDPHHLLNPG
                     VLL"
BASE COUNT          441 a          447 c          620 g          537 t
ORIGIN      
        1 cgtgatcgta aaatctggtg ggtgcgtgtc ccgtggcctg tgtgatgatg tctcgggagg
       61 tatcggggtc atcgagatag cgcgtggcgt aacaggagtc gtgaaagatt gttttggtgc
      121 ggatgttttt tttaagagcg gcgcgcaggg gtttgaggta gggttttaaa aaatcgctga
      181 tcgtgatgac ctgggcctcc aggttgaagg cgtatttggc atacgtgtgt ttgagagtgt
      241 gggcacaggc agggcaattg gtgatgatgg tcttgaattt tttgagtgaa tggaagttga
      301 tttcggcaag gtccacgaat tcatactcgg caccgcaaca gagcagggga tagccgcaac
      361 aatgaatggg gtccgcgtag agattgacga aatcgatctt gagcttgtta aacagcgcga
      421 acgtgtccct gatgacctcg gggcatttgg caattgttgt gcaggaggaa aaatagatcg
      481 cggacgcgtt ggcattgaaa tagcgcgcag ggaggaggct tctgagtttg ccggccagat
      541 cacgggcaaa ggggttgttg tgcttgtgaa accgctcgat gaaggtgttg atgggctggg
      601 gggccagttc ctgccggatc gcttctgttc tcacctgtcg caggatttgg ggcacatcga
      661 cgtggtgttc gcaggcctgc gtgcaggcgc ggcactccag acaatggtaa gcctcactgg
      721 caatttgttc gtcgaaaggc aactgtttgt gtctgacgtt attgacgagt tgcatgagtc
      781 cctggggaga atgggtcttg ttgccgtcat tgatgaagac cgggcatgcc gattgacaaa
      841 ggctggggca ggtcttgcac agattcaact gtttggaata ttcttcgaac atgttactcc
      901 tcttggccct tcagtctgtt tccggattta ttcaactgtc ggaacaggcg actcagcaag
      961 gcttggcttg ttttggcttg agtggccgtt ttctggctgg gccaaatcat gtcgtggtat
     1021 tgctgtttta tgtcctcacc agaggaagga gcataaatgt actgtgggac gatgaggcgt
     1081 tcagtttttg tttgtagcca tttgatgccg agcccctcaa aggcacacca gtcattgatg
     1141 agttcctttt gaatccccac gatttctccc agtcctgata atccgaacag gagaacgccg
     1201 caggtggtct ttttgatttt taaccgcttg gtgagttctg gctgtttttt tgtgtcgagt
     1261 tgacgaaaaa agaggggatc gaaaaaagac ccgacgaggt gtctcacaaa acgggctccg
     1321 tcttcgggtt tattcatcaa gaggagcccg tgtgttgtct cctccggttg cgtcatcacc
     1381 ctgagggtca ccagactcgc ttgtcccaac tgggagccgg acccgataaa aaggttgtca
     1441 atgtcgggac ctgttgcggc gcgcggcgcg tttttgaggt gaaccacaga cccatcgggg
     1501 agttcgcaaa gcagggaact cacgatctgg ctcaaggccc catgtttgaa atgataaagg
     1561 ttgggtgtgt tgtggtcgag atggaaacgc aggggttgcc cctgatcacc cagcggggca
     1621 tagcccaacc aacaaccttc caaggcgagt tggtgctcta tttttggcag aaacgcgtct
     1681 ggagaaacgg tgacgagcag tgatttttta ttcagttgaa acatggttac aacagcaccc
     1741 ccggattcaa caggtgatgc gggtcaaaaa attgcttcat gtgctccaac acgggcaggg
     1801 cgtctctcca ttccttgtgt gcgtgtctga ttttcaggcg gccaatacca ttgtgatggc
     1861 tgatcacccc gccgccttcc tgtaccgcgt ccagcgcgct attccagatt tcgtcgtaaa
     1921 gtttgacact gttcttgctc cccttaaggg gagcaacaaa ggtaaaataa atggaggccc
     1981 cgtcgggata cgtgtgggtg atgtgcgtga ggaccagaca atggggtgcc agttttttca
     2041 gcaca
//
LOCUS       DATDUN010000106         1120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_295582, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000106 DATDUN010000000
VERSION     DATDUN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295582"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VJC18_01515"
     CDS             complement(<1..318)
                     /locus_tag="VJC18_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44085.1"
                     /translation="MDGIQRLTIRAFAYGGTAEVVRIGEPVLVTWGELLFGFWPDTDR
                     INVAKMKGHVHTLTALRQMLDYEGLEMTTMCLTDDTGRVLGLWVPIDSAYLVPGNNMS
                     LEQS"
     gene            complement(383..811)
                     /locus_tag="VJC18_01520"
     CDS             complement(383..811)
                     /locus_tag="VJC18_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44086.1"
                     /translation="MEIRKMELADFAFGDVTSIASDGKPVLLKQGGRWFGFWPDKGRI
                     DAGKMGCFVKALEEFRQTVAVIIEVEDNLCLVDSTGQPLGLFMPIDNAYLAGTKMSPE
                     QAVLFIEEHFERHVTGLYKIAATVRSHAKNVKRKKTRVRA"
BASE COUNT          297 a          323 c          208 g          292 t
ORIGIN      
        1 tgactgttcc aggctcatgt tgttgccggg gactagataa gctgaatcaa ttggaaccca
       61 caagccgagc acccgaccgg tatcatcagt taagcacatc gtcgtcattt ccaatccctc
      121 atagtcaagc atttgccgca gtgctgtcag cgtatggacg tgtcccttca tcttggccac
      181 gttaatcctg tccgtgtcgg gccaaaatcc aaatagaagc tctccccacg tgaccagaac
      241 cggttctccg attcgcacca cttccgccgt accaccatac gcaaaagctc gaattgtcaa
      301 acgctgaatt ccatccatac ccgcacctcc ttgtcccaaa ggattaacac atcaatatgt
      361 caaacaggca aacattcatg cgtcacgccc gcacgcgcgt ctttttcctt ttcacgtttt
      421 ttgcgtggct tcttacagtc gcggcaattt tatacaatcc ggtgacatgt ctttcgaaat
      481 gttcttcaat aaacaaaacc gcttgttccg gactcatctt cgtccccgca agataggcgt
      541 tgtcaatcgg catgaacagc ccgagcggct gaccggtgct gtcaaccaaa caaagattgt
      601 cctcgacctc aataataacg gccaccgttt gtctgaattc ttcgagcgcc ttgacaaagc
      661 aacccatctt gcctgcatca atccgtccct tgtccggcca gaatccgaac caccggccac
      721 cctgctttag caagacaggc ttcccatcgg acgcgatgct tgtcacatca ccaaacgcaa
      781 aatcggcaag ttccattttt ctgatttcca tgatcacccc ttttgttaaa ggactaaaat
      841 tagcaaaaac ctacctcact attcacaaca taccaagcgc caatgttcgc gtcaagagcc
      901 gtgttgacaa aaatataact acttggtaat attttctatc gttaacagtt cgttgacaac
      961 accaacaaaa gaggtgaggc aaatgttgaa ttattgtttg tcaatctatg gctcaaagaa
     1021 tgtcagcaag aaaggcggct tttccctcct ccagctcatt atgatcacgg gcataatcgc
     1081 tttcatgacc attatcgcca tccccgtgat tttaaattat
//
LOCUS       DATDUN010000107         1348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_170980, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000107 DATDUN010000000
VERSION     DATDUN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170980"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VJC18_01525"
     CDS             <1..741
                     /locus_tag="VJC18_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44087.1"
                     /translation="VIILNGRPAAGKSEVIDFLKSVPVNERIERFHIGEFEEIDDFPI
                     LWERFEDDDLYEKFNQPRQVSNTHFTYQGQTLKGYDFKDKFFWNFLIEKLNGLYAKKI
                     RDDKHYHNTKTVLVEFARGSAHGGFKMAYDYLSDEILSKATTLYIKVSWEESLRKNRR
                     RFNPDKPDSILEHGLEDKKLEMMYKESDWDSFSGKDTNYLLVKNHKVPFGVFNNEPEI
                     TNKSAELGKHLQEVTTRMWANWIEGRNR"
     gene            869..>1348
                     /locus_tag="VJC18_01530"
     CDS             869..>1348
                     /locus_tag="VJC18_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44088.1"
                     /translation="MDQIINTNKPDSCPEDCSPSCAHVQDYRESLERTASSEHGSAPD
                     PRSKAFSLKQFGIEVYDFDRFHSTNSGTYQFVHLLDGQIDSVIAPIIQARTATSVSGR
                     ILYSYAYRSGNRAVLITDLGEDFKLEPSSPQNAVYDYFAPHTIEYAGQLLGLNALGLS
                     "
BASE COUNT          398 a          339 c          305 g          306 t
ORIGIN      
        1 gtcatcatcc ttaatggcag gcccgcggcc ggcaaatccg aggttatcga ttttctcaaa
       61 agcgttccgg taaacgaacg cattgaacgc tttcacattg gtgaattcga agaaatagac
      121 gatttcccca ttctctggga acggttcgaa gatgacgatc tgtacgaaaa atttaatcag
      181 ccccgtcagg tctccaacac gcatttcacg tatcagggac aaacgctgaa aggctacgat
      241 ttcaaggaca agttcttctg gaatttcctg atcgaaaaac tcaatggcct gtacgcaaaa
      301 aaaatccggg acgacaagca ctatcacaac accaaaaccg ttttggtaga gttcgcccgc
      361 ggatccgctc atggcgggtt caaaatggcc tacgattatc tgagcgatga aattttgtcc
      421 aaggcaacca cactgtacat caaggtgagt tgggaagaat ctttgcgcaa gaaccgcaga
      481 cgtttcaatc ccgacaagcc cgattccatt ttggaacacg gtctggaaga caaaaagttg
      541 gagatgatgt acaaggaatc cgattgggac agcttctcag gcaaagacac gaattatctg
      601 ttggtcaaaa atcacaaggt tcctttcggc gttttcaaca acgaacccga aatcactaac
      661 aagtcggcag agctgggcaa acacctccaa gaagtcacca ccagaatgtg ggccaattgg
      721 atcgagggtc ggaacaggta agatgtgcta attgtaagca cggcggcaga agcaattcgg
      781 atgacaatgt cattcctgcg aaagcaggaa tccaaataaa tagaaagttt tgacgccact
      841 ctgaatataa caacaaagat gcacaacaat ggatcagatt ataaacacaa acaaacccga
      901 ttcttgtcct gaagactgtt ctccttcttg tgcccacgtc caggattacc gggaatcact
      961 ggaacggaca gcgtccagtg aacacgggag tgcccccgat ccccgttcaa aagccttttc
     1021 cctgaaacaa tttggcattg aggtctacga ttttgatcgg tttcactcca caaattccgg
     1081 cacgtaccaa tttgtccacc tcctagatgg acaaattgat tccgtcattg cgccaatcat
     1141 acaggccaga acagcgacga gtgtctcggg gagaatcctc tattcttacg cgtacaggtc
     1201 tggcaacaga gcggtcctca tcacagattt gggagaggac tttaaactgg aaccctcttc
     1261 gccacaaaac gcggtgtacg actattttgc cccccacacg atcgagtatg ccggtcaact
     1321 tctgggcctc aatgccctgg gattgtca
//
LOCUS       DATDUN010000108         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_503422, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000108 DATDUN010000000
VERSION     DATDUN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503422"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /gene="alaS"
                     /locus_tag="VJC18_01535"
     CDS             complement(<1..1320)
                     /gene="alaS"
                     /locus_tag="VJC18_01535"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HLD44089.1"
                     /translation="MTLTGKQIRQSFLDYFASKEHTVVKSASLVPSNDPTLYFTNAGM
                     VPFKNTFTGQELRPYHRATSSQKCMRVSGKHNDLENVGRTARHHTFFEMLGNFSFGDY
                     FKTGAIGFAWEFLTEVVKLPKNKLWVTVYQDDDEAFDLWHKKIGLAKDRIVRLGDKDN
                     FWSMGETGPCGPCSEIIFEQDIPCSLNNPDCRLGNCDCDKFLEVWNLVFMQFERHADG
                     SKTPLPKPSIDTGMGLERLTALLQNKNSNYDCDLFSPIIKTIEALVQKKYTHDLTNEN
                     DVSIRVLADHIRSSVFLIGDGVLPSNEGRGYVLRRILRRAIRHGKLLGQTQPFFYKLV
                     SVVLEEMGTAYPELSTHRDTIEKVIHAEEERFLETLDKGLKLLSEEISTHQKNKQKLL
                     SGTVAFKLYDTFGFPLDLTEIIALGHGLTIDQDGFNTAMNEQKERARA"
     gene            complement(1436..>2134)
                     /locus_tag="VJC18_01540"
     CDS             complement(1436..>2134)
                     /locus_tag="VJC18_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44090.1"
                     /translation="LALTTTTSNPQQKTQTHEVRFYQVKTPTDIPLLKTLPYSEKPFV
                     FYVPDNQLVILTKTQMDLYSLSQLLEEDEVVENAEDLAPVVATSENKTETDNAQTIKP
                     IESVPLKTLSQDILQAKLSPACNKIFALVQSQKRYQILVFDIFDLTSNTLIKDFEIVP
                     SHFEGASFAWHGRIFILPTAEGTLFYDVTDLANVRELASWPVPSYHVDTARQGQFICV
                     AQGYQGVQCGTLKF"
BASE COUNT          496 a          472 c          578 g          588 t
ORIGIN      
        1 ggcacgggcg cgttcttttt gttcgttcat ggcggtattg aaaccgtcct ggtcgatcgt
       61 gagtccgtgt cccaaagcaa tgatttctgt gaggtcgagc gggaaaccaa aggtgtcgta
      121 gagtttgaag gccacagtgc ccgaaaggag tttttgttta ttcttttggt gcgtgctgat
      181 ttcctcggag aggagtttga gtcctttatc gagcgtttcc aaaaaacgtt cttcctcagc
      241 atgaatgact ttttcaatgg tgtcgcgatg cgtgctgagt tcgggatacg cggtccccat
      301 ttcttccaga accacactga cgagtttata gaagaaaggt tgtgtttgac ccagcagttt
      361 tccatgacgg atggcgcgtc tgaggatgcg ccgcaaaacg tagcccctgc cttcgttgga
      421 gggcaggaca ccgtcgccaa tcaaaaaaac ggatgaacgg atatggtcag ccaaaacacg
      481 gatgctcaca tcgttttcgt tggtcagatc atgggtgtat tttttttgaa ccaacgcctc
      541 gatcgttttg atgatcgggc tgaacaagtc gcaatcgtaa ttggaatttt tattttgcaa
      601 caacgcggtg agccgctcca agcccatgcc ggtatcaata gagggtttgg ggagaggtgt
      661 tttgctccca tcggcgtgac gctcaaactg catgaacacc agattccaca cctccaaaaa
      721 tttgtcgcaa tcgcagtttc ccaaacgaca atcgggatta ttcagtgaac aggggatgtc
      781 ctgctcaaag atgatttcgg aacaaggacc gcagggaccg gtttcgccca tggaccagaa
      841 attgtccttg tcccccagcc gcacaatgcg gtccttggcc agaccgattt ttttgtgcca
      901 gaggtcaaaa gcctcgtcat catcctgata gacggtcacc cacagtttgt ttttgggcag
      961 tttgaccacc tcggtcaaaa actcccacgc aaaaccaatg gcaccggtct tgaaataatc
     1021 gccaaaactg aaattgccga gcatttcaaa aaaagtgtgg tgacgggctg tgcgccccac
     1081 attttcgaga tcgttgtgtt tgccggagac gcgcatgcac ttttgtgagg acgtggccct
     1141 gtgatagggg cgcaattctt gcccggtaaa agtgtttttg aaagggacca tgcccgcgtt
     1201 ggtaaaatac aacgtggggt cgtttgaagg cacgaggctg gcactcttga ccaccgtgtg
     1261 ttccttggac gcaaaataat ctaaaaaact ttgtctgatt tgtttgcctg tgagggtcat
     1321 gtcgtggatc gtggatcgtg gatcgtggat cgtttcttca tcaatttatc gatccattga
     1381 cccctcaatt cactgattca ttgatttatt gtttcattga tttgttgatt cattgttaaa
     1441 acttgagtgt gccgcattgg acaccctgat agccctgtgc cacgcaaatg aactgaccct
     1501 gtctggccgt gtccacgtgg taagagggga cgggccaact ggccagttca cgcacattgg
     1561 ccaaatctgt tacgtcgtaa aataaagttc cttcggcggt ggggaggatg aatatccttc
     1621 catgccaggc aaacgaggcc ccctcaaagt gggaagggac aatctcgaag tccttgatga
     1681 gcgtgttgct ggtcagatcg aaaatatcaa agacgagaat ctggtagcgc ttttgagact
     1741 ggaccaacgc gaaaattttg ttgcacgcag gcgaaagttt ggcctgaagg atgtcctgac
     1801 tgagtgtttt aagagggacg gactcgatgg gtttgatggt ctgggcgttg tctgtttcgg
     1861 ttttgttttc gctggtagca accaccgggg ctagatcctc cgcgttttcg acaacctcat
     1921 cttcttcaag aagttgtgat aatgagtaga ggtccatttg tgttttggtc aatatgacca
     1981 actgattgtc ggggacgtaa aagacaaaag gcttctcaga gtacggaagg gtcttgagga
     2041 gagggatatc tgttggggtt ttgacctggt aaaatctgac ttcatgcgtt tgtgtctttt
     2101 gctgtgggtt tgaggtcgtg gtggtcagtg ccag
//
LOCUS       DATDUN010000109         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_295849, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000109 DATDUN010000000
VERSION     DATDUN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295849"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VJC18_01545"
     CDS             <1..369
                     /locus_tag="VJC18_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HLD44091.1"
                     /translation="LTGGEPLLQVDQKLLLALHEKGYYVALETNGTRVVPDEVDWVCV
                     SPKFGARLVATRGSELKLIYPQEGIDPQGYLDLDFKHFYLQPKDDQLHNQNRERCVAY
                     CLDHPLWKISLQIHKVLGIK"
     gene            855..1661
                     /locus_tag="VJC18_01550"
     CDS             855..1661
                     /locus_tag="VJC18_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HLD44092.1"
                     /translation="MFVFVHTDVLAGPLSDPTEMATASRLSRIISLKPNITQILMSLG
                     NADQIVGMTRFCPIPNVTAQVVADYNTIDLEKIVRLKPDLVLGSTENSQKKQYDTLEQ
                     AGIPVALFDFGTFDQMVASLKSIAGLLHKEVVADTLVEKMNGQLLQARQMLGARSGTF
                     IAIIQRQPLMVISGKTYVSTLFEQTGLQNVFGQNQIAYPVMDEEEVVREFVDHTFDLS
                     HSAQNESETFLNKKVIALRTEDFLASPQSVENLLRVLATINPGLQNMAAK"
     gene            1707..>2216
                     /locus_tag="VJC18_01555"
     CDS             1707..>2216
                     /locus_tag="VJC18_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HLD44093.1"
                     /translation="MRAFSFKLIILSFLILCGFLLFSFLGDPVISLRALFDSAATMDR
                     FVLLQLRLPKLVLAFVIGAALSVSGVSFQSLLKNPLADPYILGISGGAALGYVLAVVL
                     GLPFIIVPVAGFIFALLTLLLIYRLACTSGVLSVVNLLLIGIVFNAFSFALILLVNSL
                     ANFGQAQQIL"
BASE COUNT          550 a          472 c          549 g          645 t
ORIGIN      
        1 ctgactggtg gcgaacctct tttgcaggtc gatcagaaat tgctcttggc cctgcacgaa
       61 aagggatact acgttgccct cgagaccaat ggcacgcgag tcgtgcctga cgaagtcgat
      121 tgggtttgcg tgagtccaaa gtttggggcg cgattggtgg caactcgggg aagtgaattg
      181 aaactaatct accctcaaga ggggattgac ccacaggggt atttggatct ggattttaaa
      241 catttttatt tgcaacccaa ggatgatcag ctccataatc aaaacaggga acgttgcgtg
      301 gcctattgtc tggaccaccc tttatggaaa ataagcctgc aaatacacaa agttctggga
      361 ataaaataat aaggacatct ctaacccctc ttgtgccaag tcgtggtgtt gatgttggat
      421 gtgttgttta acgaggccac cgaagggcag agaccgggta tggttatctc aagacagaga
      481 ccatcaggca agacagaata acggcgatca cttgcatttt ttatgcaaac aattgctgat
      541 catcccagtg atcgccatcg atgcgagcag agcggtgatg gatacgaggt atatgcttag
      601 ggcaatccac attttttttc tggtttttgg ccatgccaaa cccaactgat gatgagtttg
      661 agggagtcca aaaacctgcc gtgttttttc tgttgatgtc acgattttcc tgtgtaaatg
      721 acatctttta catgctttat accctatata taatatcata acgcagcatg tcaatgtgcc
      781 gcagtgcgtc taaagagaac aaaatggcaa atattaatca tttcatacag atacatgttt
      841 tatttatttt gattatgttt gtttttgtcc acacagatgt cttggcgggg ccactatcag
      901 acccaacaga aatggccaca gcgtcccggt tgagtcgcat catttcactc aagcccaaca
      961 tcacacaaat tctcatgtcg ctaggtaacg cagaccaaat tgtgggaatg accaggtttt
     1021 gtcccatccc caatgtgaca gctcaagtgg ttgccgatta caatacgatt gatctcgaaa
     1081 aaatagtcag actcaaaccg gatctggtgt tggggagcac ggaaaattca caaaaaaaac
     1141 agtacgacac gttggaacag gcagggattc ctgtggccct ttttgatttc ggcacgtttg
     1201 atcagatggt ggcgtcgctt aagtccatcg cgggcttgtt gcataaagag gttgtggccg
     1261 atacgttagt tgaaaaaatg aacgggcaat tgttgcaggc aagacagatg cttggagcgc
     1321 gctcgggaac attcattgcg atcattcaaa gacaaccctt gatggttatt tcgggcaaaa
     1381 catatgtcag caccctgttc gaacagactg gtctgcaaaa tgtgtttggg caaaatcaga
     1441 ttgcgtatcc agtgatggat gaggaagagg tcgtccgtga atttgttgat cacacatttg
     1501 atttgagtca ttctgcgcaa aatgaaagcg agacatttct gaacaaaaaa gtgatcgcac
     1561 tcaggacaga ggattttttg gcctccccac agagtgttga aaaccttttg agagtgctgg
     1621 cgaccatcaa tcctggtttg cagaacatgg ccgctaagtg attgggacga caagttgatt
     1681 aacgcttcat tagtaataga gacaagatgc gggcattttc attcaaatta attatcctct
     1741 catttctgat cttgtgtggt tttctcctct tttcttttct gggggatccg gtgattagct
     1801 tgagggcctt gtttgattct gccgcaacga tggaccgttt tgtcctttta caactgaggt
     1861 tgcccaagct ggtgttggcc tttgtgatcg gggcggcctt atcggtttcg ggggtctctt
     1921 ttcagtctct tctcaagaac cctctggctg acccttacat attgggcatt tccggtggag
     1981 cggctttggg ttatgtgttg gcggtggtgt tggggcttcc gtttattata gtgcctgttg
     2041 ctggttttat cttcgccttg ttgacactac tgctcattta cagactggcg tgtacgagcg
     2101 gtgtgttgtc ggtggtcaac ctgctcctca tcggcattgt gttcaatgcc ttttcatttg
     2161 ccctcattct gctggtcaac tccctggcca actttggaca ggcgcaacaa attctg
//
LOCUS       DATDUN010000110         1955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_339115, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000110 DATDUN010000000
VERSION     DATDUN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339115"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VJC18_01560"
     CDS             <1..1392
                     /locus_tag="VJC18_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44094.1"
                     /translation="LDNNPVFAGLYSVGDEWDTVAIQEAINAAQPGQAVCVGPGDYKI
                     LRAEKRPRNHPFFKYEANIKFKSRLSIIGAGRNHVRFRSGTATGVENGISHDVGSTWD
                     QPIQNVTIEGISFHNLDMRLSVSTNRQDLLHHFTIKDSRFVDSTSSDFFNYEGDTMIL
                     ARYTGSVIIDNCEFLRDENHIGRGVMFYQAHDGQVINSRFDGFFITFVNVLGSTIVDG
                     EFILSQRSHNITIENNFMKRSSHLDVADQNTFLQTQLAVYNNRVATIASTFAKNGLSY
                     WENHRNTIQNIIDGQVPFSEVRYLEDHGIYAVGFSGLTIRNNRIAGNEFDQGAVKIRN
                     GEIATIEGNTADSPFYFFTYNLAQSVPYYLRQVTISDNELNLLRFENPAQQYLFNEVT
                     GCQGISYWRQGSIPGNIEIDDITITNNTIHNGCIMLRAGAYGPGITVTSNQAMDFIFD
                     VQPIASGNTVISD"
BASE COUNT          552 a          428 c          435 g          540 t
ORIGIN      
        1 ttggacaata atcccgtttt cgcggggcta tattccgttg gagatgagtg ggacactgtg
       61 gccatacaag aggctatcaa tgcggcacag ccgggtcagg ctgtttgtgt tggacccggt
      121 gattataaaa tattacgtgc agaaaaacgg cctagaaacc atcctttttt caagtatgag
      181 gcgaatatca aattcaaatc gcgcctatct atcataggcg caggtcgcaa ccatgtacga
      241 tttcgtagtg gcacggctac aggcgtagaa aatggaatat cacacgatgt tggttcaaca
      301 tgggatcagc caattcaaaa tgtgacaatc gaaggcattt catttcacaa tctcgacatg
      361 agattgagcg tgtcaaccaa tcgacaagac ttactacatc atttcaccat caaagatagt
      421 cggtttgttg attccacgtc ttcagatttt ttcaattatg agggcgacac gatgattctc
      481 gccagataca caggcagtgt gatcattgat aactgtgaat tcctacgcga tgaaaatcat
      541 atcgggaggg gtgtgatgtt ttatcaggct catgatggtc aggtcatcaa ttcacggttc
      601 gatggttttt ttatcacttt tgtgaacgtc ctgggttcta caattgtgga tggcgaattc
      661 attctcagcc agcgaagtca caacatcacg atagaaaata atttcatgaa acgcagttct
      721 catttggatg ttgcggatca gaataccttt ttgcaaaccc agcttgctgt ttacaataac
      781 agggtcgcta cgatcgccag cacttttgca aaaaacggac tctcgtattg ggaaaatcat
      841 cggaacacca tacaaaatat cattgatggg caagttcctt tttcggaagt tcgatatctg
      901 gaggatcacg gcatttatgc cgtggggttc agtgggctga ccattcgcaa taatcggatt
      961 gctggcaatg aattcgatca gggagctgta aaaatcagaa acggtgaaat agcaacaatt
     1021 gaaggcaata cggctgattc ccccttttac tttttcacat acaatcttgc ccagtctgtt
     1081 ccgtactatc tgagacaggt caccatcagt gataatgagc tcaacctatt gagatttgaa
     1141 aatcccgcac aacaatacct gttcaacgag gtcacaggtt gtcagggcat atcgtattgg
     1201 aggcagggtt ccattcctgg caatattgaa atagacgaca tcacaataac gaacaacaca
     1261 attcacaacg gctgtatcat gctgagagca ggcgcctacg gtccgggcat caccgtcaca
     1321 tccaatcagg ccatggattt tatttttgat gtgcaaccca tagccagcgg aaatactgtc
     1381 atctcagatt aatcatgcaa ctggtgccac atacctttta cacaagcttt ttctacccgc
     1441 atatcgcttg cgaatcacca tttgttgctt tggagtcagc atttccgtgt ccttagggac
     1501 acggtttcct cgtcggtcaa caccgtgtcc ctgaattgga tatcgacatc gggatcatcg
     1561 cgtaaaacga ggtcatgagg atgcagattt gaataatcaa taacacgcat ataaagagcc
     1621 ttacagcttt tatgagcaag atgcgtgcca agaaccgaag attactgtgt ttttattgtt
     1681 tgatatgatt gacgaaacct aatttcgcat aaatgaaaag caatattgaa gtcaaaattc
     1741 cgatatattc ttgttttgag aaaaaagtcg ctgtgtaatg gccggatgca agttggctgt
     1801 ccccccccct atctggcgtc tgatctttac ccataatctg acttgctggg cacagggagt
     1861 tctttttggg gcaacatcgt cgcaacaaaa atttgccagg ccgtagcaat atcatccaat
     1921 cgttgtaaac ctacccacaa ggtttggggg ccggg
//
LOCUS       DATDUN010000111         1903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_130722, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000111 DATDUN010000000
VERSION     DATDUN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130722"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VJC18_01565"
     CDS             <1..1174
                     /locus_tag="VJC18_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLD44095.1"
                     /translation="AILLITEGLFSMDSDMPDIKALQDVAREHKATLLVDVAHDFGSL
                     GTHGGGALEIQNMLGKVDLVMGSFSKTFSSNGGFLATASLAVRQYMKFYGSPQTFSNA
                     LSPVQTAVIMQALKIVRSPEGTKLREQLNNNSVTLRTGLEKNGIRCIGEPSAIVPAYI
                     GNEAVARIAWARAQNEGIHANLVEFPAVAVGSARFRMQLMPQHTQEQLDKTVSVITDV
                     IAAATKDAAKISFPKVERAKFGTDPNTLTIASHALPALKEGDMKKLFAHARTATFDPG
                     ALLIREGSLPKGLFVIKKGTVTIEVDYLGQPLVLAECGPGEILGEMSLLGSQGASASV
                     VASTSVETACLDKKELAELAKTDPELGTRFYQSLAMVLAGRLRHQNATVLPTLSHG"
     gene            1182..>1903
                     /locus_tag="VJC18_01570"
     CDS             1182..>1903
                     /locus_tag="VJC18_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta (1-6) glucans synthase"
                     /protein_id="HLD44096.1"
                     /translation="MDATNKRSFFIFITGLVLVTGLLFGVWFFLGRAHTLNEPGFPAD
                     HKLECVSYTPLIDDETPYDLGDNYQIPLSRFDQDFALLSQRFSCVRLYIMKGYEAVPL
                     LAKKYNLKMILSAWIGTDEEKNQAEISALIQTAREYQDQVLFVLVGNEVLLRREQTEG
                     QMINCVKQVKQALPQIPVSYAEVLGFWRFYKKLADEVDFLTIHILPYWDDNARSVDEA
                     REHLRFSIAYAHEQFPDKKFYVG"
BASE COUNT          460 a          531 c          482 g          430 t
ORIGIN      
        1 cgcgattctg ctcatcaccg agggattgtt ttccatggac tccgacatgc cagacatcaa
       61 ggctctgcaa gacgtggcgc gagagcataa ggccacactg ctggttgatg tggcccacga
      121 ttttggttcc ctgggcactc atggcggagg ggccttggaa atacagaata tgctgggcaa
      181 ggtcgatctg gtcatgggca gtttttccaa aaccttttcc tccaatgggg gttttctggc
      241 cactgcctcc ctggcagtgc gtcaatacat gaagttctat ggcagtcccc agacgttttc
      301 caacgccctc tctcccgtac aaacagcggt tatcatgcag gccctaaaga ttgtgcgttc
      361 gccggaaggg accaaactcc gtgaacaact caataacaac tccgtaaccc tcagaacagg
      421 actggagaaa aacggaatcc gttgcatagg cgaaccctcc gccatcgtcc ccgcctacat
      481 tggtaacgag gccgtagcac gtattgcctg ggcgcgggcc cagaatgagg gcatccacgc
      541 caatctggtg gaattccccg ccgtcgcggt gggttccgcc cggttccgca tgcaactcat
      601 gccacaacac acacaggaac agctggacaa aactgtttcc gtcattactg acgtgatcgc
      661 cgcggccaca aaggacgcgg ccaagatctc ttttcccaaa gtggaacgcg ccaaattcgg
      721 gaccgaccca aacaccctga ccattgcctc acacgccctg cccgccctca aggagggtga
      781 catgaaaaaa ctgttcgcgc acgcacgcac cgccaccttt gatccggggg ccctcctcat
      841 cagggaaggc tctctgccaa agggtctgtt cgtgatcaaa aagggcactg tgaccatcga
      901 ggtcgactat ctgggacaac ctcttgtgtt ggcagaatgc ggaccgggag aaattttggg
      961 agagatgtcc ctgctgggtt cgcagggagc cagtgcctct gtggtggcca gcaccagtgt
     1021 cgagaccgcc tgcctcgaca aaaaagagct ggcagaattg gccaaaacgg accccgagct
     1081 tggcacccga ttttatcagt ccctggccat ggtgttggca gggcgtttga gacatcagaa
     1141 tgcgaccgtt ctgcccacac tgtcacacgg ataaagccct catggacgcg accaacaaac
     1201 gttccttttt tattttcatc actggtctcg ttctggtaac aggtctttta tttggtgtct
     1261 ggtttttctt gggccgtgca cacactctca acgaacccgg gtttcccgca gatcataaat
     1321 tggaatgtgt ttcatacacc cccctgatag atgacgagac cccttacgat ttgggagaca
     1381 attaccagat cccgttgtcc cgcttcgatc aggatttcgc gctcctctcg cagagattca
     1441 gttgtgtccg tctctacatc atgaagggct acgaagcggt tccacttctg gccaaaaaat
     1501 acaatctcaa aatgatcctg agcgcctgga ttggcacgga tgaagaaaaa aaccaggcag
     1561 aaatcagcgc tctcatacaa accgcgcgcg aatatcagga tcaggtattg ttcgttttgg
     1621 tcggcaatga agttctcctg cgtcgagaac aaaccgaggg gcagatgatc aactgcgtca
     1681 agcaggtgaa acaagcccta cctcagatcc ccgtatcata cgccgaagtt ttgggattct
     1741 ggcgattcta caaaaaattg gcagatgaag tggatttctt gaccattcat attctgcctt
     1801 attgggatga caacgcccga tctgttgatg aggcacgcga gcacttgcgg ttttcgatag
     1861 cgtatgcaca cgaacaattt cccgacaaaa aattttacgt ggg
//
LOCUS       DATDUN010000112         3639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_339202, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000112 DATDUN010000000
VERSION     DATDUN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339202"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1517)
                     /gene="mnmE"
                     /locus_tag="VJC18_01575"
     CDS             complement(105..1517)
                     /gene="mnmE"
                     /locus_tag="VJC18_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HLD44097.1"
                     /translation="MRYFPDNIVAVATAPQNGAIGIVRISGVEAKNLLTHVFVSQSGL
                     AVSLVPGRLHFGRLVGVDGVVLDHCLAVYMPGPHTFTGEDVVELHCHGNLLLLKKVVA
                     AVLSLPSKAGIRGAEPGEFTKRAYLNGKVDLTQAEAVHELITASSDAALNSSLANLDG
                     ALKQLIDHMRDQLVTVLADVEASFEFSEEDIQTYNPAQTLVLVEACAQKLKTLEAAYR
                     TSKLYDHGVSVVLVGRPNVGKSSLLNAILVEDRAIVTEVPGTTRDVVEGAKIIDGVRF
                     IFRDTAGLRPAQDPIEKTGIDKTNEWIQKSDVVIVVLDHFNQEPDGEPDIMLPQKTTV
                     IRVLNKADVLEYKQKKLEQLSDSEREELQRLGGYDLVVSAKSGYQIPHIETLLKKWLE
                     HHCDKTNCLHINERQVRSISQALAKLDLCQPLLQEGGLREELLAACLRDVIGCLNDIV
                     GIVTSEDVLANIFGRFCIGK"
     gene            complement(1584..1934)
                     /locus_tag="VJC18_01580"
     CDS             complement(1584..1934)
                     /locus_tag="VJC18_01580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HLD44098.1"
                     /translation="MTKTASGLRYQDAVVGSGAVAESGKMVSVHYTGWLGSNDQKGNK
                     FDSSLDRGQPFIFPLGAGRVIKGWDEGVQGMKIGGKRVLYIPATLGYGERGAGDVIPP
                     NADLIFEVALLDVQ"
     gene            complement(2061..>3639)
                     /locus_tag="VJC18_01585"
     CDS             complement(2061..>3639)
                     /locus_tag="VJC18_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HLD44099.1"
                     /translation="LASSFFGAMIFGLFWFPRVDGFMRKNHWFFLLVVALACSLGCLV
                     VVFADRFPGFYETGFIAWIRRPHYLVGLLFLIIAIKLAGEFQRTNSFQSFLLLCLSLL
                     FSLTELAFKPFMVGCVSWWFWHGVRLLAYLAMLRFVISDYQTLVDRLGAAIIQHQKTE
                     AEIRREHAQLISIFDSMDQPIYIIDPELYQILYVNEAAKKLFGNAEGKPCHVVLHNKD
                     NPCEFCTNDKIFGNNFGKTHVWEFYNEHIGMWFRNIDKAIHWSDGRKVRFEMAVNIDD
                     VKRAEEAIKQTADIKSKFISMASHELRTPLTTIQESVSVVLDGIAGDINDKQTHMLTI
                     TKKNIQRLSRLVDDILDFQKLQSGKMPFELKECDIDQLLLEVCRPLEKTANQNKLYLK
                     YSFSKNLPLVLCDPDRIVQVVTNLVNNALKFVNRGGVMIETRQGTGFVQITVSDTGSG
                     IKKEDMPKLFQTFEQLRTSDSAVTKGTGLGLVICKEIIQGHQGKIWIESEYGRGTSVH
                     FTLPVYEEMKERITKGS"
BASE COUNT          958 a          995 c          767 g          919 t
ORIGIN      
        1 gaggaatgat ttgttgctga aaaactcatg ttcggtcttg tttatcgtaa ctcaccagta
       61 aaagttgtgt ttttttaaac ctaaaatcaa cgatttcaac ggttttattt cccaatacaa
      121 aatctgccaa agatgttggc caacacatcc tcactggtca caatgccaac aatgtcgttc
      181 aaacacccaa tgacatccct taaacatgcc gcaagcagtt cctctcgcag cccaccttcc
      241 tgcaacaggg gttggcacag atcgagcttg gccagggcct gcgaaatgct acgcacttgt
      301 ctttcgttga tgtgcaaaca atttgttttg tcgcagtggt gctctaacca tttctttaat
      361 agcgtttcga tatgggggat ctgatatccg gatttggccg acaccaccag gtcgtagcca
      421 ccaagccttt gcaattcttc tcgttcggaa tcactcaact gttcaagttt tttttgtttg
      481 tattccagta cgtccgcctt gttgagcacg cgaatgaccg tggttttttg gggaagcatg
      541 atgtctggct ccccatcagg ttcctgatta aaatgatcca gtacaacgat caccacatct
      601 gatttttgta tccattcatt ggttttgtcg atgcccgtct tttcgatggg gtcctgcgcc
      661 ggacgcaagc ccgccgtgtc tctgaaaatg aaccgtaccc cgtcaatgat cttggctcct
      721 tccaccacgt cacgcgtggt cccgggaacc tccgtcacga tcgccctgtc ctcaaccagg
      781 atggcgttga gcagtgagga cttgcccaca ttgggccttc cgacaaggac caccgacacg
      841 ccgtgatcat acaatttgga cgtccggtag gcagcctcca gtgttttgag tttttgcgcg
      901 cacgcctcta cgaggacaag cgtttgagcg ggattgtatg tttggatgtc ttcctcagaa
      961 aactcaaaac tggcctccac atcggcaagc acagtcacca actggtcacg catgtgatca
     1021 atcaattgtt tcaacgcgcc atccagattg gccaacgagg aattgagggc cgcatcggaa
     1081 ctggctgtga tgagttcatg aacagcctca gcctgcgtga ggtcgacctt gccattaagg
     1141 tacgctcgct tggtaaattc acccggctca gccccgcgaa tacccgcttt gctgggcaga
     1201 gacaaaactg ccgccacgac ttttttgagg agcagcaaat tgccgtggca atgcagttca
     1261 acaacatctt cacctgtaaa agtgtgtggg cccggcatgt agaccgccag acagtgatcc
     1321 agcacaacac catcgacacc aacaagcctc ccaaaatgca gccgccccgg gaccaggctc
     1381 accgctaaac cgctttggct cacaaagaca tgcgtcagaa gatttttggc ctcgacacca
     1441 gaaatacgca caatgccaat ggccccgttc tgtggggccg tcgccacggc cacaatgttg
     1501 tctggaaaat accgcatatt ctctctgcgc tctgcaaagg aacctttgaa atcagtgcag
     1561 aaatgattgc ttgcaaaatg gcatcactga acatcaagca gggccacttc aaatatgaga
     1621 tcggcattcg ggggaatcac atcgccagcg cccctttctc cgtagcccag cgtggcgggg
     1681 atgtacaaaa cccttttgcc accaatcttc atgccctgca caccctcatc ccagcccttg
     1741 atcacccggc ccgcacccaa gggaaaaatg aatggttgtc cgcgatccag ggaactatca
     1801 aacttgttgc ctttttgatc gttgctcccc agccagccgg tgtaatgcac actgaccatc
     1861 tttcctgatt cggccacagc accagacccc acaacagcgt cttgatacct caaacctgac
     1921 gctgttttgg tcatgtcagc agaataaaca ttaacagcca acaacacaag cagtccagtc
     1981 atcaacagaa tgattttttt catacaaact tcctttcgtg cgggatatct attcacttaa
     2041 aaaattgatt gcaaacgata ttatgaaccc ttcgtaatac gctccttcat ttcctcataa
     2101 acaggcaaag taaaatggac agaggtcccc ctgccatatt cggattcaat ccatattttc
     2161 ccctgatgcc cctgtatgat ttccttacaa atcaccaacc ccaagcctgt ccctttggtc
     2221 acagcactat ctgatgttcg gagctgttca aatgtttgaa aaagttttgg catatcctct
     2281 tttttaatgc cactccccgt gtcactcacg gtgatctgca caaatcctgt gccctgtctt
     2341 gtttcaatca taacccctcc acgattgaca aatttgaggg cattattgac caggttggtc
     2401 accacttgca caattcgatc cggatcacaa agcaccaagg gaaggttttt gctaaagctg
     2461 tatttcaaat acaacttgtt ctgattggct gtcttttcga gggggcgaca tacctccaga
     2521 agcaattgat caatatcgca ctctttgagt tcgaaaggca ttttgccaga ctgtaatttt
     2581 tgaaaatcca gaatatcatc caccagccgg gatagtcgtt gtatgttttt ttttgtgatc
     2641 gtgagcatgt gcgtctgttt gtcattaatg tcaccggcaa tgccgtcaag caccacactc
     2701 acagactcct gaatcgtcgt cagcggcgtc ctgagttcat gggacgccat ggaaataaat
     2761 ttggatttga tatcggcggt ttgtttgatg gcctcttccg cccttttcac atcgtcaata
     2821 ttcacagcca tttcaaaccg aaccttcctg ccgtcggacc aatgaatggc tttgtcaata
     2881 ttgcgaaacc acatcccgat gtgttcattg tagaattccc agacatgtgt ttttccaaaa
     2941 ttgttcccaa atattttgtc gttggtacaa aattcacagg gattgtcctt gttatgcaaa
     3001 acgacatggc acggcttccc ctccgcattc ccaaacaact tcttggccgc ctcattcacg
     3061 tacaggattt gatagagttc ggggtcgatg atgtaaatgg gttggtccat gctgtcgaag
     3121 attgaaatga gttgggcgtg ttcccgccga atttccgcct ctgttttctg atgctgaatg
     3181 atcgccgccc ccaaacggtc aacaagtgtc tggtaatcgg aaatcacaaa gcgcaacatg
     3241 gccagatagg ccaataacct gaccccgtgc caaaaccacc acgacacaca ccccaccatg
     3301 aagggcttaa aggccagctc agtcaaacta aacaaaaggg acaaacacaa caggagaaaa
     3361 gactgaaaac tgttggtcct ctgaaattca ccggccaact tgatggcaat gatcagaaac
     3421 agcaacccca caagataatg cggacgcctg atccaagcga taaatccggt ttcgtaaaag
     3481 ccgggaaatc ggtctgcaaa aaccaccacc agacacccca gggaacaagc caaggccacc
     3541 actaacagaa aaaaccagtg gttttttctc ataaagccgt caacacgtgg aaaccaaaac
     3601 aaaccaaaga tcatggcccc aaaaaaactc gaagccaac
//
LOCUS       DATDUN010000113         1170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_339317, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000113 DATDUN010000000
VERSION     DATDUN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339317"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VJC18_01590"
     CDS             complement(<1..423)
                     /locus_tag="VJC18_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44100.1"
                     /translation="MNFVGRQREQNLLQSVEIVNKSKLTVLYGRRRVGKTSLIETTFS
                     KNRLIKFEGLEGESSQAQKENFIRKCQQLFPGQFHLARTTNHWIDLLVLFSQCLGTTP
                     CIVFFDEFQWLANNRKQLVSAFKYVWDNHFAKENHVHMI"
     gene            complement(578..>1170)
                     /locus_tag="VJC18_01595"
     CDS             complement(578..>1170)
                     /locus_tag="VJC18_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44101.1"
                     /translation="GELKHFIKLGKKNPVLIKLADFFASGIGPVAAAYGLTRYLCQHK
                     PDLIITGNTAGIINSKKFAVGDLVLAKTITPCARSKESFCPSIIKPIVAVPNKVIRHF
                     QLTMPKIKTASVFCPQEISCSLSLGRRLKLAGFEVETLETYAYHHVAKKFDIPIVSFL
                     GLTNLVGPNAHAEWKKNEAFVCQKLARVIATLISIR"
BASE COUNT          302 a          252 c          289 g          327 t
ORIGIN      
        1 gatcatgtgc acgtggttct ctttggcaaa gtgattgtcc caaacatatt tgaaggcact
       61 gaccagttgt ttgcggttgt tggcgagcca ctgaaactcg tcaaaaaaca caatgcacgg
      121 cgttgtgcct aggcattgtg aaaacaatac gagcaaatca atccagtggt ttgtggtgcg
      181 ggccagatga aactggccgg gaaaaagttg ctgacacttt ctgatgaagt tttctttctg
      241 tgcctgagag gattccccct ccaatccttc aaatttgatc agacggtttt tggaaaaagt
      301 cgtctctatc aggcttgttt ttcccacacg tcgtcgacca tagagcacag tcaacttgga
      361 tttattaaca atctcaacag attgcaacag attctgctct ctttgacggc caacaaagtt
      421 cataaacagt gtccttctgt gttaaatgtt atcacaggcg gacgaaaatc aaaagtcaaa
      481 tttcgtccgc ctgaagactg tttttgtatt gaaggacagc aaattctttg gaacagtctc
      541 ctgaatgatt cgacaattcg ggagtgtagt cgtgttatta tcgaattgaa atgagtgtgg
      601 cgatgactct tgccagtttc tgacagacaa aggcctcgtt ttttttccat tcagcatgcg
      661 cgtttggtcc taccagattg gtcaggccca aaaaagaaac gatcgggatg tcgaactttt
      721 tggccacgtg atgataggcg taggtctcca gggtttccac ctcaaatccg gcaagtttca
      781 aacgccgtcc aagcgataag gaacagctga tttcctgcgg acaaaacaca ctggcggttt
      841 tgattttggg catcgtcagc tgaaagtggc ggatgacctt attcggtacc gccacaatag
      901 gtttgatgat gctggggcag aaagactctt ttgaacgggc gcaaggtgtg atggttttgg
      961 cgagcaccag gtctcccaca gcgaattttt ttgaattgat gatgccagct gtgttgccag
     1021 tgatgatcag atctggtttg tgttgacaca gataccgagt caagccataa gcggcggcca
     1081 cagggccgat gccagaggcg aaaaaatccg ccagcttgat caagacggga ttttttttgc
     1141 caagtttgat gaaatgcttt agttctccct
//
LOCUS       DATDUN010000114         2509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_503694, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000114 DATDUN010000000
VERSION     DATDUN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503694"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VJC18_01600"
     CDS             <1..837
                     /locus_tag="VJC18_01600"
                     /inference="COORDINATES: protein motif:HMM:NF027969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HLD44102.1"
                     /translation="NFGVQELLDAFIKYAPAPLAREAESRLVDPLEKDFTGLVFKIQA
                     NMDPKHRDRCAFIRICSGKFEPGMMVYHNRLQREIKLHNAVQFMSRERKNIEDAFAGD
                     IIGMIDRGTFTIGDTLSMGEELKFTGVPQFSPDLFALASLRNPIKMKQLNKGLEHLAE
                     EGSSQYFRRRYNSDSIIGVVGRLQFEVVKYRLLHEYGADALFQNLPYTCSRWYRSEDR
                     KALEKFEDYYQGQIVFDVRDYPMMLFKDDWEHNYVAGKFPEIRFYSSLLNYEKGQVTG
                     DR"
     gene            879..1472
                     /locus_tag="VJC18_01605"
     CDS             879..1472
                     /locus_tag="VJC18_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015336273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HLD44103.1"
                     /translation="MNYAQIFPIALSLFLIMDPLGNTPVFLAILKDHSPKRQRRIVLR
                     ETVFALVFILGFYFIGETVLTYLNIEQSSIRISGGIILFIMAIKMIFPQLEKNNSDLY
                     TEDPFIVPIAIPLIAGPSLIATISLYAHQVHNDLIVLMAILVAWVGNLVVYSAMTLLK
                     NVLKERGLKACERLMGLILMMISVQMFLDGLTLYRKA"
     gene            1687..2022
                     /locus_tag="VJC18_01610"
     CDS             1687..2022
                     /locus_tag="VJC18_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44104.1"
                     /translation="MKQKWIMIVAVLLVLSFAVSCAGRTPTAKSAQSIGTHYFQSYGK
                     KFPFSDFGHKNIDRVMINGVEEVSFRMALVDAVVQHVDGHASHVLMKMEKKFPGGWCV
                     ISWEMAGQR"
     gene            complement(2032..>2509)
                     /locus_tag="VJC18_01615"
     CDS             complement(2032..>2509)
                     /locus_tag="VJC18_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44105.1"
                     /translation="SFITEALPFPSRKLDAIITVLTLPVHMTLVVLTSSQTVWEVILI
                     GFIMECGIILATIVYLVFEKIAQGSWNGCAIIILLGTAGVLLAAGIPLLLTMIENNWW
                     VYLLSVATAGAGIAQTIYHYKFSPKPMLVSEKFIALGVGAFIVLPLAAILVRNIWG"
BASE COUNT          651 a          561 c          646 g          651 t
ORIGIN      
        1 aattttggcg tgcaggaact gctcgacgcg ttcatcaaat acgcgccagc acctctcgcg
       61 cgggaagccg aatcacgcct tgtggaccct ctcgaaaaag attttacggg tctggtgttc
      121 aaaattcagg ccaacatgga tcccaaacac cgcgaccgtt gtgccttcat ccgcatttgt
      181 tcgggcaagt ttgaaccggg gatgatggtg tatcacaatc gtttgcaaag ggagatcaag
      241 cttcataacg ccgtgcagtt catgtcgcgc gaacgcaaga acatcgagga cgcctttgcg
      301 ggagacatca ttggcatgat cgatcgcggc acgttcacga tcggtgacac cttgtcaatg
      361 ggagaggagc tcaagtttac gggcgtgccc cagttttcac cagatctttt tgcgctggcc
      421 agcctcagaa atcccatcaa gatgaaacag ctcaacaaag ggcttgaaca tctggccgaa
      481 gaagggtcat ctcagtattt ccggcgacga tacaattccg acagcatcat cggcgtggtg
      541 gggcggttgc agtttgaggt cgttaaatac cgacttctgc acgagtacgg ggcggacgcc
      601 ctcttccaga acttgcctta cacgtgttcg cggtggtatc gctcggagga cagaaaggcc
      661 ttggaaaaat tcgaagacta ttatcagggt caaattgtgt tcgatgtgag agattatccc
      721 atgatgctgt tcaaggacga ctgggaacac aattacgtgg cgggcaaatt tccagaaatc
      781 aggttttatt cgagtttgct gaattacgag aaaggacagg taacaggtga caggtgacaa
      841 aaagactgtt gaccgttagc tgttggcagt ttgccattat gaactacgca caaatttttc
      901 ccatcgccct ctcattgttt ctcatcatgg acccgctggg gaacacgccg gtgtttttgg
      961 cgatcctcaa ggatcacagc cccaaacgtc agcgtagaat tgttttgagg gaaacagttt
     1021 ttgcgttggt cttcattttg ggtttttatt tcattggcga gacggtgctg acgtatctga
     1081 atattgaaca atcgagcatc aggatctcgg ggggcatcat tttgttcatc atggcgatca
     1141 agatgatttt tccccagctc gaaaaaaaca acagcgattt gtacacggaa gacccgttca
     1201 ttgtgcccat cgccatcccc ttgattgccg ggccatcact gattgcgacc atatcgttgt
     1261 atgcccatca ggtgcataat gatctgattg ttttgatggc tatactggtg gcgtgggtgg
     1321 ggaatcttgt tgtttattct gccatgacct tgttaaagaa cgttctcaag gaaagagggt
     1381 tgaaggcctg tgagcggctc atgggactca tcctcatgat gatttcggtc cagatgtttt
     1441 tggatggttt gacgttgtat aggaaggcgt aaggcgtagg gcatttgtaa tatcgaatat
     1501 cgatcagaga atattgaatg ttgaaatttg tcatccctgc gaacgcaggg atccagaatt
     1561 aatgtaaata tggattccag cctacgctgg aatgacaatc acttcgaaat tccttgttcc
     1621 ttgttcatta ttcgatattc gatccccttt gagctgtctt cgtgtgcacg tcaacaaagg
     1681 ggagatatga aacaaaaatg gataatgatt gttgccgtgc tgttggtgct tagttttgca
     1741 gtgtcctgcg cggggcggac gccgacggcc aaatcggcgc agtcaatcgg gacgcactat
     1801 tttcaatcct atggcaagaa attcccgttt tctgattttg gacacaaaaa catcgatcgg
     1861 gtgatgatca atggcgtcga agaggtgagc tttcgcatgg cgttggttga tgctgtggtc
     1921 cagcatgtgg acgggcacgc gagtcacgtc ctgatgaaga tggaaaagaa gtttcccggt
     1981 ggctggtgcg tgatttcgtg ggaaatggcc gggcagagat agggatgcgg gttaccccca
     2041 aatgtttcgc accaatattg cggccaacgg caagacaata aacgcaccga cccctagtgc
     2101 tatgaatttt tcggatacta acattggttt tggtgaaaac ttgtaatgat aaatggtttg
     2161 agcgattccc gcaccggccg tggcaacaga caacaaataa acccaccaat tattttcaat
     2221 cattgtcaat agcaacggaa tgccagccgc gagcaacaca ccggctgtgc ccagtaatat
     2281 aataatagcg caaccattcc acgacccctg ggcaattttt tcgaatacca gataaacaat
     2341 cgttgccaaa atgatgccac actccatgat aaaacctatc aaaatgactt cccaaacagt
     2401 ttgtgaggaa gtcagcacaa ctaaggtcat gtgtaccggc aaagttagta ccgttatgat
     2461 agcatccaat ttgcgcgatg ggaagggaag cgcttcggta ataaacgac
//
LOCUS       DATDUN010000115         1386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_544305, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000115 DATDUN010000000
VERSION     DATDUN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544305"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1386
                     /locus_tag="VJC18_01620"
     CDS             <1..>1386
                     /locus_tag="VJC18_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44106.1"
                     /translation="ILLDTKERLARHLTACANNHGKFHLSLAPELARFLSACLAKRCF
                     GVSGLEYFLSKLEEKQVAFDQLRFDFEAYELHWELVKRLPRTQRNSFLSVANSHFYKF
                     GTRMRFARALYDNGLSQLVATPGSKLNPQAVQLLELATLITMDPWCLEHDLEHALDEY
                     CQQGRVRQSAHTYRMFHVMQNKYRAEIYSDPKLTQALGRILSRTSLDLDVKTVILAAI
                     FTPTTPLDQSTTQDRLRFFGDEILLGVCASHIKQMATRFYWGRSTLTELKEDLFIFAR
                     LRQRRSASLPDDLADRYYQFIDNPITYRSCQAYSSETDDQSFDSSVLTNRQRGILFLL
                     NYLFGWPQGRSVAKGILFNLSYQPPEYQEDDPAILLGIILDAVHDFIGLRHAKGACQD
                     LAEDPFLIHYFSSRHYLAHPEEERVGYYTLCVPRMSQQNTYREYRSLIMEFCAQVWAF
                     KHLPGFNPLPGE"
BASE COUNT          317 a          410 c          317 g          342 t
ORIGIN      
        1 attcttctag acacaaaaga gcgtctggca cgtcacttga cagcttgtgc caacaatcac
       61 ggcaaattcc acctgtccct ggcgccggaa ctcgcgcgtt tcctgtcggc ttgtcttgcc
      121 aaacgctgtt tcggagtctc cggtctggaa tattttttgt ccaaactgga agagaaacag
      181 gttgccttcg atcaattgcg ctttgatttt gaggcctacg aactgcattg ggaactcgtc
      241 aaacgcctgc cccgcaccca acgaaacagt tttctgtctg tggccaacag ccatttttac
      301 aagttcggca ccagaatgcg ctttgccagg gccttgtacg acaacggtct gtcacagctg
      361 gttgccacac ccggctctaa gctcaaccct caggccgtcc aactcctgga actcgcgaca
      421 ttgatcacga tggacccttg gtgcctggaa catgatctgg aacatgcact cgatgaatac
      481 tgtcaacagg gtcgggtgcg gcaaagcgcc cacacctacc gcatgtttca tgtcatgcaa
      541 aacaaatacc gtgcagaaat ctattccgat ccaaaattga ctcaggccct gggacgaatc
      601 ctctcccgca cctctcttga tctggacgtc aagacagtca tcctggcggc catttttaca
      661 cccaccacgc ctttggatca atccacgaca caggacaggc ttcgcttctt tggcgatgaa
      721 attctcttgg gggtatgcgc gtcccacatc aaacagatgg ccacccgttt ctactggggc
      781 aggtcaacct tgacagaact caaagaagat ttgttcattt tcgcgcgcct ccgccaacgg
      841 agatcagcca gcctgccaga cgatctggct gacagatatt accagttcat tgacaatccc
      901 atcacgtacc gttcgtgtca ggcgtattca tctgaaaccg atgaccaaag ctttgattcc
      961 tcggtgctga caaaccgcca acgtggcata ctttttctgt tgaattatct ctttggctgg
     1021 ccacaggggc ggtcagtggc caagggcatc ctgttcaatt tgtcttatca accccccgag
     1081 tatcaggagg acgatccggc catcctgttg ggcataattc tggacgccgt gcatgacttc
     1141 atagggcttc gtcacgccaa aggcgcctgt caggacctcg cggaagaccc ttttctcatc
     1201 cattatttca gctcccgtca ttaccttgct caccccgaag aagagcgggt cgggtattat
     1261 accttgtgtg ttcctcgcat gtcacaacag aatacctaca gggaataccg ctcgctcatt
     1321 atggaattct gcgcccaagt ctgggccttt aaacatctgc cgggattcaa tcccctgccc
     1381 ggcgaa
//
LOCUS       DATDUN010000116         1829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_461688, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000116 DATDUN010000000
VERSION     DATDUN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461688"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1829
                     /locus_tag="VJC18_01625"
     CDS             <1..>1829
                     /locus_tag="VJC18_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLD44107.1"
                     /translation="RAHLRQNDPATRVKTYDEVDHALTENETKCESARCLSCGCSAVF
                     TCDLKKVATDYRVDQKKYAGKVNKFRVDATHPHIVLDPNKCIVCGKCVRLCDEVLGIG
                     ALGYVRRGFEMVVKPALEKPLAETNCTSCGNCVEICPTGALSLKMPCTQPGPFKTTTY
                     DSVCSLCGSNCALVYHKVNDDIWTVGGKPINQYTQGLICQRGRFGQHNALRTNRLTSA
                     RRTQQGKTAPCSMDEAINALAQGLLTTHKTQGPEAMGFLISPTATNEVTYLFQKLARE
                     VFLSNQVSSLSDLTQDHIIPQLIDSLGMTGSALTPNDLDQTHVIVLMNSDITEDSPVL
                     SYSVKQAVRNGAKLISLSSANFDINKQASLWLNTRKGSHATLLQTVCGELIRQNKHDI
                     NYLKANTIGWETFCQNQTLSIETAVQECGVTREQIRVLIDLLGNSEANIAFLFNPYSP
                     SDGTPDDLGIIINYLMLTGRSSKASNGLMLVHEHGNRQGHINYGGYVEVYAHNAHVAK
                     QNGLQGVKTSSELRDKLLSNQIKSLFVWDEDVASEPELAAIFKNTPFTATVTPHDSPT
                     AKMAKLVLPGTLPAESEGTLTDQYRCQRPFTRVFAPPSGLTGF"
BASE COUNT          486 a          521 c          432 g          390 t
ORIGIN      
        1 acgggcccat ctccgccaga atgaccccgc cacgcgtgtc aagacctatg acgaagtgga
       61 tcacgccctc accgaaaacg agaccaagtg cgaatcagcg cgttgcctgt cctgcgggtg
      121 ttccgctgtc tttacctgtg acctcaaaaa agtcgccacg gattatcgcg tggatcaaaa
      181 gaaatacgca ggcaaggtca acaaattccg tgtggatgcc acacacccgc acattgtcct
      241 cgaccccaac aagtgcattg tctgcggaaa atgcgtacgg ctttgcgatg aagtgctggg
      301 catcggtgcg ttggggtatg tgcgtcgcgg gtttgaaatg gtggtcaaac cagccctcga
      361 aaaacccctc gctgagacca actgcacctc atgtggcaac tgcgtggaaa tctgtcccac
      421 aggggcgctg agtctcaaaa tgccctgcac tcaacccggt cccttcaaaa ccacaacata
      481 tgattctgtt tgttcgcttt gcggttcaaa ctgcgccctc gtgtatcaca aggtcaatga
      541 cgatatctgg accgtgggcg gcaaacccat caatcaatac acacagggac tcatctgcca
      601 aagaggccga tttgggcaac acaacgcatt acgcaccaac agattgacca gcgcacgcag
      661 gacacagcaa ggcaagacgg cgccctgctc gatggatgag gccatcaacg cgttggccca
      721 agggttgctg acaacacaca aaacacaggg tcccgaagcc atgggtttcc tgatttcgcc
      781 aaccgccacc aacgaagtga cctatctgtt tcaaaaactc gcccgtgagg tgttcctgtc
      841 aaatcaggtc agcagtctct ctgatctgac acaagatcac atcattcccc agttgattga
      901 ttctcttggc atgacgggat cagctctcac gccaaatgat ctggatcaaa ctcacgtgat
      961 cgtcctcatg aactctgaca tcaccgaaga cagtcccgta ctctcttact cggtcaaaca
     1021 ggctgtcaga aacggcgcca aattgatctc tctgagcagt gcgaactttg acatcaacaa
     1081 gcaagccagt ctctggctca atacgcgcaa aggttctcac gcgactctgc tccagacagt
     1141 gtgtggtgaa ctcatcaggc aaaacaagca cgacataaat tatttgaagg caaacacaat
     1201 cgggtgggag accttctgtc aaaatcaaac tctttcaata gaaaccgccg ttcaagaatg
     1261 cggcgtgacc agagaacaga tacgggttct gattgatctc cttggcaaca gcgaagcaaa
     1321 catcgctttt ctctttaacc cctattcccc ttcggatggg acaccagacg atctgggcat
     1381 aatcatcaat tatctgatgc taacgggacg atcctcaaag gcatcaaatg ggcttatgct
     1441 ggtacatgaa cacggcaacc gacagggaca catcaactac ggtggttacg tggaggttta
     1501 tgcccacaat gcgcacgtgg caaaacaaaa tggtctccag ggagtcaaaa catcatctga
     1561 attgcgagac aaactcttgt caaaccagat caaatcgttg tttgtttggg atgaggacgt
     1621 cgcctcggag cctgaactgg cggccatttt caaaaacacc ccgttcacag ccacggtgac
     1681 accgcacgat tcacccacag ccaagatggc caaactggtt ttgcccggca cgctccccgc
     1741 tgaatcagag ggcacgctga ctgatcaata ccgctgtcag cgtcctttta cgcgtgtgtt
     1801 cgcaccccca tcgggcttga ctggttttg
//
LOCUS       DATDUN010000117         2665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_420845, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000117 DATDUN010000000
VERSION     DATDUN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420845"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VJC18_01630"
     CDS             <1..790
                     /locus_tag="VJC18_01630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HLD44108.1"
                     /translation="VNIILNLKKLRLKLLENEYTKMRLCVTGAGVVTASAFETGGLVE
                     IINPDLEIATLSEDAKIELEVDVEMGKGYVLAEENKKESAPIGTIFIDSHFAPVTKVN
                     YTVTNARVGQVTDYDRLNFEVWTNGAITPEDAVAFAAKILKDQIQVFINFDEFEEPAE
                     EVVVHVESGLNDNLNRRVDELELSVRSANCLQNANIRFIGELVQKSEMEMLRTKNFGR
                     KSLNEIKEILSEMGLGLGMKIGEAWRPPEGAQAVKKIAEEELEY"
     gene            816..1385
                     /gene="rplQ"
                     /locus_tag="VJC18_01635"
     CDS             816..1385
                     /gene="rplQ"
                     /locus_tag="VJC18_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HLD44109.1"
                     /translation="MRHQVAGRKLGRTTAHRLAMFRNMLASLIQHGRIKTTLPRAKEL
                     RSLADKIVTLGKENTLHARRRAFDFLRDRKAVVRLFNDIAPAFSGRMGGYTRIYQLGS
                     RVGDAAKMAMIEFLQEDLLAAKVAGNIVDNKAKKKAGKKAATQKEDVATGTVKEAGEA
                     KEKTKVVKTKKTAEKKENKTKKSSTKTKE"
     gene            1480..2352
                     /locus_tag="VJC18_01640"
     CDS             1480..2352
                     /locus_tag="VJC18_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HLD44110.1"
                     /translation="MSHQNIIKLGIVQMKCTEDLKQNRDQAVEQIRILAAQGAQIICL
                     QELYSSLYFCQQQDERFFELAEPVPGPTTQNLQAVAKKLGVAIVASLFEKRSAGLYHN
                     TAAVIDADGSYLGKYRKMHIPDDPCYLEKYYFTPGDLGYQVFRTKYARVGVLICWDQW
                     YPEAARLTALKGAEIIIYPTAIGWLKNEESALLAKQYNAWKAVQTGHAIANEVFVVAP
                     NRVGEEENIRFWGGSFVVDPFGETLYQAPHDKEESVVVSCNLSRIETYRRNWPFFRDR
                     RIDSYGDITKRMDD"
BASE COUNT          749 a          563 c          712 g          641 t
ORIGIN      
        1 ggtgaacatc attctcaatc tcaagaagct tcgcttgaaa ctactcgaga atgagtacac
       61 aaagatgaga ttgtgcgtga cgggcgcggg tgtggtgacg gcatcggctt ttgaaacagg
      121 cgggttggtc gaaatcatca atcccgatct tgaaatagcc accttgtccg aggatgcaaa
      181 gatcgaattg gaagtcgatg tggaaatggg caaggggtat gtgttggccg aagaaaataa
      241 aaaggagagc gctccgatcg gtacgatttt catcgattct cactttgctc cagtgaccaa
      301 ggtcaactac acggtgacca acgctcgcgt aggtcaggtg acggattatg accgactcaa
      361 ttttgaagtt tggacgaatg gcgccatcac tcctgaagat gctgttgcgt ttgccgccaa
      421 aattttgaaa gatcagatac aggtttttat caattttgac gaatttgaag agccagcaga
      481 ggaggtcgtg gtgcacgttg agagcggttt gaacgataac ctcaatcgcc gcgtggatga
      541 actggaactt tcggttcgtt cagccaactg tttgcagaat gccaacatcc gtttcattgg
      601 tgaactggtc caaaaatcag agatggaaat gttgcgcacc aagaattttg gtcgcaagtc
      661 acttaatgaa atcaaagaaa tcctgtccga aatgggcttg ggtttgggaa tgaagattgg
      721 cgaggcctgg agacctccag aaggtgccca ggcagtcaag aagattgccg aagaagaatt
      781 ggaatattaa tcacagataa cactgagggg tatttatgag acaccaagtt gctggtagaa
      841 aactgggcag gacaacagcc caccgtttgg ccatgtttcg gaatatgttg gcgtccttga
      901 ttcagcacgg ccgtatcaaa acaacactgc cgcgggccaa ggaattgagg agtctggccg
      961 ataaaatcgt cactctgggc aaggagaata ctctccacgc ccgacggaga gcctttgatt
     1021 ttctgcgcga ccgtaaggct gtggtcagat tgtttaatga tatcgccccc gctttctctg
     1081 gaagaatggg cgggtacacc cgtatttacc agttgggatc gcgcgttggt gatgccgcca
     1141 agatggccat gattgagttt ttgcaggaag atctgttggc cgccaaggtg gctggtaata
     1201 ttgttgacaa caaggccaag aaaaaggcgg gcaaaaaggc agcgacgcaa aaagaggacg
     1261 ttgccacagg aacagtcaaa gaagccggtg aagccaaaga aaagactaag gttgttaaaa
     1321 caaaaaaaac agctgagaaa aaagaaaata aaaccaaaaa gtcttcaacg aaaacgaagg
     1381 aataaattac agctgagctg caggaaatca gaaaagccaa ggacgaaact ccttggcttt
     1441 tttcgtttga atggataggc ctttgttggg gaatgatcta tgagtcatca aaatattatc
     1501 aaattgggca ttgtgcagat gaaatgcacg gaagatctga agcaaaatcg tgatcaggca
     1561 gtagagcaga tcagaatact tgcggctcaa ggggcccaga tcatctgttt gcaagaactc
     1621 tattctagtc tgtatttttg tcagcagcag gatgaacgtt tttttgaatt ggcggaaccg
     1681 gtgcctggtc ccacaactca aaatctgcag gccgtggcca aaaaactcgg tgtggcaatc
     1741 gtggcgagcc tgtttgaaaa aaggtctgcg ggactttatc acaacactgc ggcagtcatc
     1801 gatgccgatg gttcgtattt gggcaagtac cgcaaaatgc atattcccga tgacccctgt
     1861 tacctcgaaa aatattattt taccccaggt gatttgggat accaggtttt tagaacaaag
     1921 tatgctcggg ttggcgtgtt gatctgctgg gaccagtggt accctgaggc ggctcgtctc
     1981 acagccctaa aaggcgctga gatcattatt taccccactg ccattggatg gctcaagaat
     2041 gaggagtcag cacttttggc gaagcaatac aacgcgtgga aggctgtgca gacaggacac
     2101 gccattgcca atgaagtctt tgtggtggcc ccaaatcgcg tgggagaaga ggagaatatt
     2161 cgtttttggg gcgggtcgtt tgtggtcgat ccctttggcg agaccctgta tcaggccccg
     2221 cacgacaaag aagaatctgt tgtggtttca tgcaacctgt ctcgcatcga aacataccgc
     2281 aggaattggc cttttttcag ggatcggcgg atcgattcgt atggtgacat cacgaagagg
     2341 atggatgact aactcatgcg cttgtgttca tgtgtttacg tgtttatgtg ttcacgtaaa
     2401 atacattttt ttgaacagcc gaacacgata ttatgcaacc acagaatttt atgaatagtt
     2461 cacgatccat accctctggg gcacaagcga tccacgaccc acgattcacg aatttacgca
     2521 tgcccgcaga atgggaaccg caggaggcca tcattctttc ctggccgcat aatgagctga
     2581 cctggcggac acacattttg cctcaggttg aggccatcta tgtgcgtctg attgaagaaa
     2641 ttgcagagca ccagaaggtc tggct
//
LOCUS       DATDUN010000118         1600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_89973, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000118 DATDUN010000000
VERSION     DATDUN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89973"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VJC18_01645"
     CDS             <1..977
                     /locus_tag="VJC18_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44111.1"
                     /translation="RPDIVSTPNSNIVAHNLSVLSHVRPTGSTTNGGGGLSEGVILGS
                     GQVQHCEFSLDPAGRLVGAVRWVDHSLRDSTYSQGTATDTMVKASIVFWGYGGSAVIL
                     DYQTRGNLTHTQRANWGGTFAAQEAIRSAFIAENPYALEVINRTPQALLPEDIVDALH
                     LGIEALSVYSFSKKWQQKERHLLLLNVGDQVRIIRVFAEGEIQTRARRVYLLGSGLKV
                     LAGLRVVITKQTPHVYKDTPDPTPPLQLRLILPKGFTHYDLIMPIFELVQRKLVSTHL
                     QVKGWESATSAYASNVQIEFTDDNLIPANHVDADGNYRSSPYRNSNGE"
     gene            1033..>1600
                     /locus_tag="VJC18_01650"
     CDS             1033..>1600
                     /locus_tag="VJC18_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLD44112.1"
                     /translation="MKTKLTVIFFVSLFVSLPYTSFAKTDIEFIVDVSGSMKKVSGQE
                     TQIASAVNALKQTLQTLPDEVTLALRVYGHREGQEDKAKSCRDSELVVPFAPSNKALI
                     DQKTQNLTPKGYTPIAFSLEQARADFDATREAQKVIILLSDGEETCDGNPVAVLEKLK
                     QDGFNVIVHTIGFNVDDKAKQQLINIAQA"
BASE COUNT          408 a          411 c          412 g          369 t
ORIGIN      
        1 ccaggcccga catagtatca acccccaatt caaacatcgt tgcgcacaac ctgagtgttt
       61 tgtcgcacgt acgcccgaca ggatcaacaa ccaatggagg gggaggtctt tctgaggggg
      121 tgattttagg atctggacag gtgcaacatt gtgaattcag tttggatcct gctggtcgcc
      181 ttgttggtgc tgtgcgttgg gtggaccata gtctgcgcga ctccacgtat tcgcagggca
      241 ccgcaacgga tactatggtc aaggcctcta tcgtattttg gggatacggt ggctcagccg
      301 tcatcctcga ttatcagacc cggggtaatc tgacccatac acaacgggca aactggggag
      361 gcacgtttgc cgctcaagag gcaataagga gtgcatttat agccgagaat ccctatgcct
      421 tggaggtcat taacagaaca ccacaggcct tgctcccgga ggacattgtg gacgcgcttc
      481 acctggggat tgaggctctg tctgtgtata gcttcagtaa gaaatggcaa caaaaagaac
      541 gccatctttt attgcttaat gtgggtgatc aggtacgcat cattcgggtc tttgctgagg
      601 gggaaattca gacacgcgca cgtcgagtat acctgttggg ttcggggctt aaggttttgg
      661 ctggtttaag ggtggtcatc accaagcaaa ctccacacgt atataaggat acccctgacc
      721 ctaccccacc gctacagttg cgcctgatcc tgccaaaggg tttcacacat tatgatctga
      781 tcatgcccat tttcgaattg gttcaaagga aactggtcag cacccacctg caagtcaaag
      841 ggtgggagag tgccacgtct gcttatgcaa gcaatgtgca aattgaattc acggatgaca
      901 atctgatccc tgcgaatcat gtggacgctg atggcaacta ccgcagttca ccataccgaa
      961 actcaaatgg tgagtgacat tcgattcatg attcatgatt gaccttgatc cccattcggg
     1021 tatgaggacg tcatgaaaac aaaactcacc gtcatctttt ttgtttcgtt atttgtcagc
     1081 cttccataca cctcttttgc caaaacagac atcgaattca ttgttgatgt atcgggttcc
     1141 atgaagaaag tgagcggaca ggaaacgcag attgcaagcg cggtcaacgc cttaaaacag
     1201 accctgcaga ccctgcccga tgaggtcacg ctggccctgc gagtgtacgg tcatcgcgag
     1261 ggacaggaag acaaggccaa gagctgtcgc gattcggagt tggtggtgcc atttgcgccc
     1321 tcaaacaagg cgctgatcga tcaaaaaacc caaaacctca cccccaaagg gtacacgccc
     1381 atcgcgtttt ccctggaaca ggccagagcc gattttgacg ctacccgcga ggcccagaag
     1441 gtcatcattt tgctcagtga cggcgaggaa acctgcgatg gcaaccctgt ggccgtgctg
     1501 gaaaagctga aacaggacgg gtttaacgtc atcgtgcaca ccatcgggtt caacgtcgat
     1561 gacaaagcca aacaacagct catcaacatc gcccaagcct
//
LOCUS       DATDUN010000119         2575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_379913, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000119 DATDUN010000000
VERSION     DATDUN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379913"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VJC18_01655"
     CDS             complement(<1..399)
                     /locus_tag="VJC18_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HLD44113.1"
                     /translation="MNLTFKELKTLFPHHTLTLADDVCVAGISRDTRNIKPGDIYVAI
                     KGETHDGHDYVAVAFEKGAVAALVENVPVKRDNLIIVPHVIRALGELAAYYRRKFTQP
                     LIAITGSNGKTTTKELLAHVLSQQGLVSATQ"
     gene            complement(518..2068)
                     /locus_tag="VJC18_01660"
     CDS             complement(518..2068)
                     /locus_tag="VJC18_01660"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HLD44114.1"
                     /translation="MLLSKLLHNIVPLPPSPDIEITHLCNDSRQARRGSLFFARQGAQ
                     VDGAQFITAAQKGGAVAIIHDATTPANLDIPAIRVGDINAVQANIAARFFDHPSTRFH
                     LCGITGTNGKTTITYLLEKFWSPARTGVIGTVNFRYGEQVLPAPNTTPDSLTLQSLFA
                     DMVQHKVTHVALEVSSIGLEQKRTKGCQFDSAVFTNLTQDHLDYHQSMEEYYQAKKLL
                     FTEGMSSSSKKNKLAVINIDDPAGERLCRELAGSHLPIETFSTAGKQATASLEGADYQ
                     IEQTRASVLIKGKKHTLTTNLLGEHNLKNILTSLLVGWHSGCDMPSMLKQLVHFTVPG
                     RLERVPHTRCFVDYAHTPDALTNVITAIRQVLQKEDVHARLLVVFGCGGDRDHTKRPL
                     MGAIVACLSDVSIVTSDNPRTESPRKIIDDILPGITKNQSPFEGTKGYLIIEDRRQAI
                     HHALELATAKDVVLIAGKGHEDYQIIGTQKTHFDDREVVREWMEKRKHENTRDEGRRN
                     AKCKMKGL"
     gene            complement(2074..>2575)
                     /locus_tag="VJC18_01665"
     CDS             complement(2074..>2575)
                     /locus_tag="VJC18_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLD44115.1"
                     /translation="AQKFDTRLKAYAENQYISSFIGFTPLEDPRLAIFIAYDTPRKNG
                     YYGGLVAGPVFKALAEETLKYMGEVSREQANTTTKIAQNKTDAKTNLAIVTPEQKWTS
                     AQKDLSQNKVPNLKGLSLRQVLTLTQGTPVQLTVKGSGIVVKQMPESGDLTNTKEFVL
                     TLGERT"
BASE COUNT          539 a          612 c          703 g          721 t
ORIGIN      
        1 ctgggtggca ctgaccagac cttgctggct caatacgtgc gcaagcaatt ctttggtagt
       61 ggtttttccg ttggacccgg taatcgcgat gaggggctgg gtgaatttgc ggcgatagta
      121 cgcggccagt tcaccgaggg ctctgatcac atggggcaca atgatcagat tgtcgcgttt
      181 gacagggaca ttttccacca gagccgcgac ggcccccttc tcaaaagcga cagcgacata
      241 atcgtgcccg tcgtgtgttt ctcccttgat ggcgacataa atatcacccg gtttaatgtt
      301 gcgggtgtca cgggaaattc ccgctacgca aacatcatcc gcaagggtga gggtgtgatg
      361 gggaaacagg gtttttaatt ctttgaaggt caggttcata atatagttca aaactcaaag
      421 gggaaaacga atatcgaata atgaacaagg aacaaggaat ttcgaagtaa tggcccccta
      481 tttcgacatt cgacattcct tgatcgatat tcgatattca taggcctttc attttgcact
      541 ttgcatttct tcgtccttcg tccctcgtgt tttcgtgttt tcgtttttcc atccattccc
      601 tcaccacctc ccgatcatcg aagtgggttt tttgtgtccc gataatctgg tagtcttcgt
      661 gtcccttgcc ggcgatcagg accacatctt ttgccgttgc cagctccagc gcgtggtgaa
      721 tggcttgtcg cctgtcctcg atgatcaaat aaccttttgt tccctcaaag ggggactggt
      781 tttttgtgat gccgggcaaa atgtcgtcaa tgattttacg gggcgattct gtgcgtggat
      841 tatcactggt gacgatgctc acatcggata ggcatgcgac aatggcaccc atcagggggc
      901 gttttgtgtg atcccgatct ccgccacacc cgaacacgac cagcaaacgg gcatgaacat
      961 cttccttttg caacacctgt ctgatggcgg tgatgacgtt ggtgagggcg tccggcgtgt
     1021 gcgcgtaatc cacaaaacac cgtgtgtgtg gtaccctctc cagacggcca ggaaccgtaa
     1081 aatgcacgag ttgtttgagc atggagggca tgtcacaacc actgtgccac cccacgagca
     1141 gggacgtcag gatatttttc aagttatgtt cacccaaaag atttgtggtg agggtgtgtt
     1201 ttttcccctt gataagcaca gaggccctgg tttgttcgat ttgataatcg gcgccctcaa
     1261 gactagccgt agcctgttta cctgccgtgg aaaaggtttc gatgggcaga tgactgccgg
     1321 ccagttcccg gcacaggcgc tcaccggcag gatcatcgat attgatgaca gccagcttgt
     1381 tttttttgga actggaactc attccctcgg taaacaaaag cttcttggcc tgataatatt
     1441 cttccatgct ctggtggtaa tcaaggtggt cctgtgtgag gttggtaaaa acggcgctat
     1501 cgaattggca acccttggtg cgtttttgtt cgagcccgat agaggaaacc tccagggcaa
     1561 catgagtcac tttgtgttgg accatgtcgg caaaaagact ttgcaaagtg agtgagtctg
     1621 gtgtggtgtt gggggctggc agtacctgct ccccgtagcg gaaattgacc gttccgatca
     1681 cacccgttcg cgcagggctc cagaattttt ccaacaggta agtgatggtt gtttttccgt
     1741 ttgtccctgt gatgccgcac aaatgaaacc gggttgaggg gtggtcaaaa aaacgggctg
     1801 ctatgttggc ttgtacggca ttgatgtccc cgaccctgat ggccgggatg tccaggtttg
     1861 cgggcgttgt ggcgtcgtgg atgatggcca cagccccccc tttttgggct gcggttatga
     1921 actgggcacc atcgacctgg gcaccctgtc tggcaaagaa caggcttcct cgtctggctt
     1981 gtctggagtc attgcaaaga tgtgtgatct cgatatctgg cgatggcgga agaggaacaa
     2041 tgttatgtaa gagtttcgat aacagcatca ttgtcaagtt cgctcaccga gcgtgagaac
     2101 gaattccttt gtgttggtca aatctcccga ttccggcatc tgcttaacca cgatgccgct
     2161 ccctttgaca gtgagttgga ccggtgtgcc ttgagtcagt gtgagcacct gcctgagact
     2221 caatcctttc aaattgggaa ccttgttttg gcttaaatct ttttgagccg aagtccattt
     2281 ttgttctgga gtcacgatcg ccagattggt ttttgcgtct gttttattct gggcaatttt
     2341 tgttgtggtg ttggcctgtt cgcgtgagac ttctcccatg tatttgagcg tctcttcggc
     2401 aagtgctttg aatactggtc ctgcgaccaa tccaccgtaa tagccatttt tgcggggagt
     2461 gtcgtaagcg atgaaaatgg ccagccgcgg gtcctccagg ggggtgaagc cgatgaacga
     2521 actgatgtat tggttttcgg cataggcctt gagccgggtg tcgaatttct gggct
//
LOCUS       DATDUN010000120         1289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_504036, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000120 DATDUN010000000
VERSION     DATDUN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504036"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VJC18_01670"
     CDS             complement(<1..180)
                     /locus_tag="VJC18_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44116.1"
                     /translation="MEIKPIKKVRTVHYSGIEQEDLLQRPQIKTTLKDNPQATGFEYA
                     QRRLYWNGQEVGQVIR"
     gene            complement(189..542)
                     /locus_tag="VJC18_01675"
     CDS             complement(189..542)
                     /locus_tag="VJC18_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44117.1"
                     /translation="MKRITDINDHQHPSTTNKHHLANTTPGKPHKRMGYYDGKIFFAS
                     DRATYTFVHEDAVVILHLDVARSSLYLNGHKITSMNEQDELPEFLERFKKCLASNPQT
                     QSLLHSFDSIVLKLE"
     gene            806..>1289
                     /locus_tag="VJC18_01680"
     CDS             806..>1289
                     /locus_tag="VJC18_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44118.1"
                     /translation="MTTAGQACIDAISDPFKDYSKVQMGSETFYVDAQGRRVSASKLQ
                     QLEAKKEEAARQCAGGAGQLAFGLGNAGYLAWEFAERFQARNVEADGYTNRPVMTFPK
                     PSLNILRGYEGNPGLTRFGGWVEVFRGGYGGVMGLADAFNPPVFYSQELGDLPSFMAG
                     D"
BASE COUNT          337 a          270 c          335 g          347 t
ORIGIN      
        1 cctgatcacc tgtcccacct cctgaccgtt ccagtagagg cgcctctggg catattcgaa
       61 ccccgtggcc tgtggattgt ctttgagagt cgtttttatc tgaggtcttt gcagaaggtc
      121 ctcctgctcg atgccggagt aatgaacagt gcggactttc ttgatgggtt ttatttccat
      181 tgggaatgtt attccaattt gagcacaatg gagtcaaacg aatgaagcag gctctgcgtt
      241 tgcgggttac tggccagaca tttcttaaaa cgctcaagaa attccggcag ttcgtcttgt
      301 tcgttcattg aggtaatctt gtgaccgttt agataaagcg aagaccgcgc gacatcaaga
      361 tgcaaaatga ccacagcatc ttcatgaaca aaggtgtatg tggcacgatc gctcgcaaag
      421 aaaatcttcc cgtcgtaata ccccattcgt ttgtggggtt tgccaggcgt tgtgttagcc
      481 agatgatgct tgtttgttgt tgatggatgt tgatgatcgt tgatgtcggt aattctcttc
      541 ataaaaacaa ccccctactc atcaaaaagc agtttccgtg ccaaattcca aaaaaaagaa
      601 aagaaggtaa tcagatgaaa taattaaggt ttatcattat gaaaaaaacg acagataggc
      661 aaaaacttct cattttcaaa attcattctc ttaatgagag agaagtaaag gctgtctgac
      721 ccaccacgtc ataaacgctc catcgtgcgc aacttttctt cgacttgtga cgataatcgg
      781 ttgtaggaaa aaaataggta caactatgac cacggcagga caagcatgca tcgatgcaat
      841 cagcgatcct tttaaagatt attcaaaggt tcaaatgggt tcggagactt tctacgtcga
      901 tgcgcagggg cggcgtgtct cagcaagcaa acttcagcag ttagaggcaa agaaagaaga
      961 agccgcaagg caatgcgcgg gtggggcggg tcagcttgcc tttggtctcg gcaatgcggg
     1021 gtatttggct tgggaatttg cggagcgttt tcaagcgcgt aatgttgagg ctgatgggta
     1081 caccaacaga cccgtgatga ctttccccaa accatcacta aacatattga gagggtacga
     1141 agggaatccg ggattaacca ggtttggcgg gtgggtcgag gtctttcgtg gtgggtatgg
     1201 cggggtgatg ggtttggccg atgctttcaa cccgccggtg ttttattcac aggaattggg
     1261 tgatctccca tcgttcatgg ccggtgatg
//
LOCUS       DATDUN010000121         1893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_6220, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000121 DATDUN010000000
VERSION     DATDUN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6220"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VJC18_01685"
     CDS             complement(<1..252)
                     /locus_tag="VJC18_01685"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HLD44119.1"
                     /translation="MKIFTSTSDAKSFFKKGSAIAIGNYDGIHLGHQAILNALKKTAK
                     KLHLKTGLLTFEPHPAKVLAPNAALQLINTREQKIEILRD"
     gene            complement(353..772)
                     /locus_tag="VJC18_01690"
     CDS             complement(353..772)
                     /locus_tag="VJC18_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HLD44120.1"
                     /translation="MAITSRHLIKNLLCPSRMMTYSWPEAKPPIDSNYRAEHRLMVNA
                     DKTIRCTACMLCVTVCPADCIHIVPAEGNHPATEKYAKEYSIDTLRCVYCGLCVEACP
                     CDAIRMDTQKLASVACEREAFVRNIDELVRGRDERRF"
     gene            1010..>1893
                     /locus_tag="VJC18_01695"
     CDS             1010..>1893
                     /locus_tag="VJC18_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44121.1"
                     /translation="MKNRKHIKLVLGFFTVLLILYMAGVVFVSHFRSFEFEDGVYQSS
                     QKNLLVNSELARLRVALSVYHQALLDYLVTGNPDQTTVLQVYGNGLIKKLTWLEGFNR
                     ELFSPWIESTTDNVINSRAALKQLIDAQGAVGVLPRQGEDRLAQTSGQFQKIFYLLKS
                     NTGFYLDQSRNLIRRFDTNPDADFQLSSLDEVREQLALLQATINALSRDYDAFFWSEQ
                     HFQDSQYYEYRQFVFVGLIIASVLVLLVFVVVSWQVRRFVYAYHEIDAQNILLNTRDL
                     ETGLFNKQSFQVFGAQELA"
BASE COUNT          433 a          407 c          486 g          567 t
ORIGIN      
        1 gtcccggagt atttcgattt tttgttcgcg ggtgttgatg agttgcaggg cggcatttgg
       61 cgccagaacc ttggcggggt ggggctcaaa cgtgagcagg cctgttttga ggtgaagctt
      121 tttggcggtt tttttgagtg cgttcaaaat cgcctggtga cccaggtgga tgccgtcgta
      181 gttgccaatc gcgatggcgg agcctttttt gaaaaacgat ttggcgtcgg aggtgctggt
      241 gaatattttc attattcgac attccctgat cgatatttga tattcatacg cttttcactt
      301 ttcattttgc attttgcact ttgcactttg catttctttg ttccgtcgtt ggtcaaaaac
      361 gcctctcgtc acgccctcgc acaagctcat caatatttct aacaaaagcc tctctttcac
      421 aggccaccga agcaagtttt tgcgtatcca tgcggatggc gtcgcaggga caggcttcca
      481 cgcacaaacc gcaatacaca cagcgtagtg tgtcgatcga atattctttg gcgtatttct
      541 ccgttgcggg atgattccct tcagcaggga cgatgtgaat acagtccgca ggacacacgg
      601 tcacacaaag catgcaggct gtacagcgaa tcgttttgtc cgcattgacc atcaagcgat
      661 gctctgcacg atagtttgaa tcgatgggcg gtttcgcttc gggccaggaa taggtcatca
      721 tgcgagaggg gcacagtaga tttttgatga gatgacgcga ggtgattgcc ataccgcgta
      781 ggatttcaaa aaaggggacc aggctccaaa gggaggatct gtagcggggg acctgtttaa
      841 tcattttgac attttgtgcg aaattgttct gtgttgagcg ttatataggt tgcactaaat
      901 tcgtcaaatt attagtatca cttgcgtatt ttgtttgtca ggagaaaaca ggttgcaagt
      961 cttgtctttt tttgtaagtg gcacaaaata cacaaaaatt ccccttacta tgaagaacag
     1021 aaagcacatc aaacttgtct tgggtttttt tacagttttg ctcattcttt atatggcagg
     1081 ggttgttttt gtttcccatt tccgcagttt tgaattcgaa gacggagttt atcagagttc
     1141 acagaagaac ctgctggtga acagtgagtt ggctcggtta cgtgtagcct tgtctgtgta
     1201 tcatcaggcc ttgctggatt atctcgtgac aggcaaccct gatcagacga cggttttgca
     1261 ggtctatggt aacgggctga tcaaaaaact gacttggctc gaggggttca accgtgagtt
     1321 gttttcaccc tggattgagt caaccacaga caatgtgatc aattccagag ccgcgttgaa
     1381 gcaactgatt gatgctcaag gggcagtggg tgttcttcct cgtcagggtg aagatcgcct
     1441 tgcgcagaca tcaggacaat ttcaaaaaat attttatctg ctcaagagca ataccgggtt
     1501 ttatctggat cagtctcgta acctcatcag gaggttcgac accaatcccg acgcggattt
     1561 tcaactcagt tccctcgatg aagtccgcga acagcttgcc cttttgcagg cgacgatcaa
     1621 tgccctcagt cgcgattatg atgccttctt ctggagcgaa cagcattttc aggattcaca
     1681 gtattatgag tatcgccagt ttgtttttgt gggactgatc atagcgtctg tgctggtctt
     1741 gcttgtgttt gtcgttgtgt cctggcaggt gaggcgcttc gtgtacgcct accacgagat
     1801 cgacgcgcaa aatattctgt tgaacacacg agatcttgaa acggggttgt tcaacaaaca
     1861 gagttttcag gtttttggtg cgcaggaact tgc
//
LOCUS       DATDUN010000122         1005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_421279, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000122 DATDUN010000000
VERSION     DATDUN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421279"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1005)
                     /gene="trmFO"
                     /locus_tag="VJC18_01700"
     CDS             complement(<1..>1005)
                     /gene="trmFO"
                     /locus_tag="VJC18_01700"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HLD44122.1"
                     /translation="HTDKPAELVCSNSFRGVDVKNAVGLLKEEMAKTDSLIMQSAQKA
                     QVPAGGALAVDRDVFSDEVKARLLSFENLTYRVDVVQGFERQPQGFLVICESGQHIVC
                     DRVVVATGPLTDATLANWIRGQTGREHLYFYDAIAPIVEVDSIDMNVAFRANRWGKSC
                     IVDRRSSIEKGERCPEGDYINCPLNREEYEAFISAIKNAELIPVKEFDDPKFFDGCLP
                     IEEMVRRGDDVLRFGPMKPVGLFIQGSRDRRVAGSQGHSFCVCDPALPRPREPFPHAV
                     VQLRQDNLHDTLYNMVGFQTRMKYHEQERIFRMIPGLEKAQFVRLGSLHRNTFMCSPV
                     L"
BASE COUNT          250 a          298 c          230 g          227 t
ORIGIN      
        1 caaaactggt gagcacataa acgtgttgcg gtgcaaagat cccaaacgaa cgaactgcgc
       61 cttttctaac cccgggatca tgcgaaaaat tctttcttgt tcatgatatt tcatccgcgt
      121 ttgaaacccc accatgttgt acagcgtgtc gtgcagattg tcttgacgga gttggacgac
      181 agcgtgggga aatggttcgc ggggacgcgg aagcgcggga tcgcagacac aaaaagagtg
      241 accctgcgac cctgcgaccc tgcgatcccg cgatccttgt atgaacagcc ccaccggctt
      301 catgggccca aagcgaagca catcgtcacc ccgccgcacc atttcttcaa tgggcaaaca
      361 cccgtcaaaa aatttggggt cgtcgaattc cttgaccgga atgagttcgg catttttgat
      421 ggcagagatg aacgcctcgt actcctcacg attcaggggg caattaatat aatccccttc
      481 gggacatcgc tccccttttt cgatcgacga tcgacgatcc acgatgcacg atttccccca
      541 ccgattcgcc cggaacgcca cattcatatc gatgctatcg acctctacaa tcggcgcaat
      601 ggcgtcgtaa aaatacaaat gttcccgacc cgtttgtccc cggatccaat tggccaatgt
      661 tgcatcagtc agtggcccag tcgccaccac aaccctgtca cagacaatgt gttggcccga
      721 ttcacaaatc actaaaaatc cctggggttg tcgttcaaat ccctgaacaa cgtccacacg
      781 gtacgtcagg ttttcaaaag aaagcaggcg cgccttcacc tcatccgaaa acacgtcacg
      841 atccacggcc agagccccac cggcgggaac ttgtgctttt tgcgcagact gcatgatcag
      901 tgaatccgtc ttggccattt cttccttcag aagccccacc gcatttttca catcgacgcc
      961 acgaaacgaa ttggaacaaa ccaattctgc tggtttgtcg gtgtg
//
LOCUS       DATDUN010000123         1516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_504175, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000123 DATDUN010000000
VERSION     DATDUN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504175"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="VJC18_01705"
     CDS             complement(<1..1450)
                     /locus_tag="VJC18_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HLD44123.1"
                     /translation="MTKSIVTKTVLVVLLVFCTLLFSQDVFAATIHVDETDCNDVDGF
                     PNFCTITAAIGYATTGDHIYVHAGTYDGNVSVNKSLHILGATSNAVIVDGGATTVVFS
                     ITTPGLTVELENLTIQNGVGSGISATAATTLTISNSTIRNNSATFGGGLIVTNVGSTT
                     TISNSTFSGNSATNGGGAIKNGGTMTIVNSTIAENNSDSDAGDITDTGSDGGAVYNTS
                     MLTIKNTIIANNTGVASKSPDCFGDIASTGYNIVGNEENNCDVDAAVGDQLGTIDNPI
                     NPLLGALADNGGSTQTYALLTGSPAIDAIPSTSCTLDEDQRGYDRPAVADQNCDIGAY
                     EAGICGDSAVDYNETCDDGNVTDGDGCSDVCQLESCGNGFVDPGEACDDGDADDTDGC
                     KSTCTLPVCGDDITNGVEECDDGNTTNGDGCNASCEIVVCGNDSDDPGEECDDGNTVS
                     WDGCSATCELESCGDGVLDTGEVCDDGNSVAGD"
BASE COUNT          390 a          404 c          343 g          379 t
ORIGIN      
        1 cgtcaccggc gacggaattg ccgtcatcac agacctcacc ggtatcgagc acaccatcac
       61 cgcacgattc gagttcacaa gtggcggaac agccgtccca ggaaaccgta ttgccatcat
      121 cacattcttc gccggggtca tcagaatcgt taccacagac cacgatctcg cacgatgcat
      181 tgcaaccatc accgttggtg gtattgccat cgtcacattc ttcaacaccg ttggtgatgt
      241 catcaccaca gacgggcagg gtacaagttg atttacaacc atcggtgtca tcggcatcgc
      301 catcgtcgca ggcctcaccg gggtccacaa aaccattgcc gcacgattcg agctggcaaa
      361 cgtctgagca accatcaccg tcggtgacat tgccatcgtc acaggtttcg ttgtagtcca
      421 ccgcgctatc accgcagata cccgcctcgt aggcaccaat atcgcagttt tggtctgcca
      481 ctgctggacg atcgtatccg cgctgatctt cgtcgagagt gcatgacgtg gagggaatgg
      541 catcaatggc cggactacct gtcaacaagg catacgtctg cgtgctccca ccattatcgg
      601 caagagcgcc gaggaggggg ttgatgggat tatcaattgt gcctaactga tcaccgactg
      661 cggcatccac gtcacaatta ttctcttcat taccaacaat attataccca gtcgaagcaa
      721 tgtcgccaaa gcaatctggt gattttgatg caacgcctgt attattggca atgatggtgt
      781 ttttgatggt cagcatgctt gtattataca ccgccccacc atcggaccca gtatctgtaa
      841 tatcgcccgc atcagaatca gaattatttt ccgctatcgt ggaattaacg atcgtcattg
      901 tcccaccatt tttgattgcc ccaccgccat tcgttgcgct gtttccagag aaggttgaat
      961 ttgatattgt ggttgttgaa ccgacattag taactattaa tccaccacca aacgtcgcag
     1021 aattgttcct gatcgtgctg ttgctgatag tcagggtcgt tgccgctgtg gccgatatgc
     1081 cactacccac cccattctga atggtaaggt tttcaagctc aacggtgagg ccgggggtgg
     1141 tgatagaaaa cacaacggtt gtggcaccac catcaacaat aacagcgttg ctggtggctc
     1201 ctaaaatatg cagtgattta ttgacggaaa cgttcccatc ataagtgccc gcatgcacat
     1261 aaatatgatc ccccgttgta gcatacccta tcgccgcagt gatggtgcaa aaatttggaa
     1321 aaccatcaac atcattgcaa tctgtttcat ccacatgaat cgtcgccgca aacacatctt
     1381 gagaaaaaag aagcgtgcaa aaaaccaaca aaaccaccaa aacagttttt gttacaatag
     1441 attttgtcat aataattctc cttgtattcg tggatcgtga atcgtggatc gtggatcgtg
     1501 gatcgacggt caaagg
//
LOCUS       DATDUN010000124         3012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_339749, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000124 DATDUN010000000
VERSION     DATDUN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339749"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VJC18_01710"
     CDS             <1..592
                     /locus_tag="VJC18_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HLD44124.1"
                     /translation="NLLIRLHQNEFCFDLGRCSLKGLHNHENMLASLSAAIIIDSSPT
                     ALASYQNTLANFVGLPHRMQKIHSSNGIDYYDDSKATNVDSVVMALASFDGNVILIAG
                     GQSKHCDYTPLKGIMHHKVKTLILLGEAQDEMHDAFHDQAPTERVANMREAVTLAKRL
                     ATSGDVVLLSPACASFDQYQNYKERGNDFQQCAKTL"
     gene            589..1770
                     /gene="ftsW"
                     /locus_tag="VJC18_01715"
     CDS             589..1770
                     /gene="ftsW"
                     /locus_tag="VJC18_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HLD44125.1"
                     /translation="MIFQRKQKKAEADKWMFFMPPRDNAVTVQQPFDVYLFFTTLMLI
                     LIGVLMIFSSSAILAQEKYGDTYYFLKKELVFFTIGLGALFLARRIPYKSYEKWIYPL
                     LGLNLLMLVLCLVPPFGYTAGGASRWIKLGSFTLQPSEITKILVVFFVAYMIAKKKEK
                     MISFAKGFLPTMAIAGIFLAVILAQKDLGSAFVLGSVIMIMLFVSQTRSLYLISSLLL
                     ASPALYYLIFSVSFRKRRILAFLDPWKYKMDYAFQIIQSFVAFKSGGLAGSGLGESKQ
                     KLFYLPEAHTDFIFSVLGEEFGMIGVIVVASLFLFFIFRGFRIANKTTDPFGMFLALG
                     ITSLIGLQAFINFGVVMGLLPTKGLVLPFISYGGSSLVISMFAVGVLLNVSTQTGEEV
                     E"
     gene            1876..2964
                     /gene="murG"
                     /locus_tag="VJC18_01720"
     CDS             1876..2964
                     /gene="murG"
                     /locus_tag="VJC18_01720"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HLD44126.1"
                     /translation="MTNTALHNKLLIAGGGTGGHIFPGLAIAEEWRQQGGRVIFVGTE
                     SGKESKLVPAHGFEIRFIQVGHLKGTSLVNKMKTLWHLPRAVVTAMKIIRQEKPDVVL
                     GIGGYVSGPTCLAAKLLGFPTAITDQNALPGFTNRLLGKIVKKIFTSFAESATYFDPV
                     KVVCTGNPTRAKIQHTAYTRPAEVFCILIFGGSQGAVTLNKIVTTALGQLPEPRTKLK
                     IFHQAGETDQADIETFYQQLNIRATVSRFFNNMAELYAQAHLVICRSGAGSVTELAMS
                     GRPAIFVPYPFAADNHQKKNADVCVAANAGWCFEQKDLNADKLADLLTELFQHPDELS
                     RRAANMLTLARPQAAKDIVTELQSLSVR"
BASE COUNT          718 a          797 c          735 g          762 t
ORIGIN      
        1 caacctgctc atccgtctgc accagaatga attctgtttt gatctgggtc gctgttcgct
       61 caaaggactg cacaatcacg aaaacatgct ggcctcgctg agtgcggcca tcatcatcga
      121 cagctcaccg acagccctgg cgtcatatca aaatactctg gcaaattttg ttgggctccc
      181 gcaccgcatg caaaaaattc attcctcaaa cgggatcgac tattatgacg attccaaggc
      241 caccaacgtg gattccgtgg tcatggccct ggccagcttc gatggcaacg tcatcctgat
      301 cgccggcgga cagagcaaac actgcgatta cactccgctc aagggaatca tgcaccacaa
      361 ggtcaagacg ttgatcctgc tgggagaggc ccaggatgag atgcacgacg cctttcatga
      421 ccaggccccc acagaacggg tagccaacat gcgcgaggcc gtcacgctgg caaaacgttt
      481 ggccacgtca ggcgacgtgg tcctgctctc tcctgcctgt gccagttttg atcagtatca
      541 gaactataag gagagaggaa acgactttca acagtgcgcc aagacgttat gatttttcaa
      601 agaaaacaaa aaaaagccga ggccgacaaa tggatgttct tcatgcctcc ccgcgacaac
      661 gcggtcaccg tacaacagcc gttcgacgtg tatctgtttt tcacaacctt gatgctgatc
      721 ctcattggcg tcctgatgat cttttcgtcc agtgccattc tggctcagga aaaatatggg
      781 gacacctatt attttctcaa aaaggaactc gtatttttca cgatcggtct gggcgccctg
      841 tttttggccc gtcgcattcc gtacaaatcc tacgaaaaat ggatttaccc tcttctggga
      901 ctcaatctgc tcatgctggt cctgtgcctt gtccccccct ttggttatac agccgggggc
      961 gccagccgct ggatcaagct gggctcgttc accctgcaac catctgagat caccaaaatc
     1021 ctggtggtgt ttttcgtggc ctacatgatc gccaagaaaa aagaaaaaat gatctctttc
     1081 gcaaaaggtt ttttacccac catggccatt gccgggattt tcctggctgt tatcctcgca
     1141 caaaaagatc tggggtccgc ctttgtgctg ggttcggtca tcatgatcat gttgttcgtt
     1201 tcccaaacgc gtagcctgta cctcatcagc tcgctgttgc tggcaagccc tgcgttgtat
     1261 taccttattt tttctgtttc gttcagaaaa cgtcgtatcc tcgcctttct ggatccctgg
     1321 aaatacaaaa tggattacgc gtttcagatc atccagtcct tcgtcgcctt caaatcagga
     1381 ggactcgcgg gatccggtct gggagaaagc aaacaaaaac tgttttatct gccggaagct
     1441 cataccgatt tcattttttc ggtcctggga gaagaattcg ggatgatcgg tgtcattgtg
     1501 gtcgcctccc tgtttttatt ttttattttc agagggttcc gtatcgccaa caaaaccacg
     1561 gatccctttg gcatgtttct ggccctgggc atcacatcgt taatcggttt gcaggccttc
     1621 atcaattttg gggtggtcat gggtttgttg cccaccaagg ggctggttct gccgtttatc
     1681 agttacgggg gatcttctct cgtgatctcc atgttcgccg tcggggtgct gttgaacgtc
     1741 tcgacacaaa cgggagaaga ggtggagtaa ggaatatcga atttcgaaca aggaacaagg
     1801 aattttgaag tgtgatgtat tcatttcgac attcgacatt ccttgttcga tattcgatat
     1861 tcaattgctg tgatcatgac taatactgca ttacataata agctcctcat cgccggtggt
     1921 ggcaccggag ggcatatttt tcccggactg gccattgccg aggaatggcg tcagcagggc
     1981 ggccgtgtca tttttgtggg cacagagtct ggcaaagaat ccaagttggt tcccgctcac
     2041 ggctttgaga tccgtttcat ccaagtcggg cacctcaagg ggacatcact ggtcaacaaa
     2101 atgaaaaccc tgtggcacct cccgagggcg gtcgtcacag ccatgaaaat catccgacag
     2161 gaaaaaccgg atgtcgtgtt gggcattggc gggtatgtct ccggacccac ctgtctggcc
     2221 gccaaacttc tgggcttccc cacggccatc acggaccaga acgccctccc cggtttcacc
     2281 aaccgcctgc tgggcaaaat cgtcaaaaaa atattcacca gttttgcaga aagtgctacg
     2341 tatttcgacc ctgtgaaagt ggtgtgcacg ggaaacccca cgagggcaaa gattcagcac
     2401 actgcctaca ccaggcccgc ggaggttttt tgcattctga tttttggagg ctcacagggg
     2461 gccgtcacac tcaacaaaat cgtaaccacg gcgttggggc aactgcctga accgaggaca
     2521 aaattgaaaa tttttcatca ggcgggcgaa accgaccagg cagacattga aacattttat
     2581 caacagctca atatccgcgc cacggtcagc cgctttttta ataacatggc cgagctatac
     2641 gcccaggccc atctggtgat ctgccgctct ggggcaggct cagtcacaga attggccatg
     2701 agcgggagac ccgccatttt tgttccctac ccgttcgcgg ctgacaatca ccagaagaaa
     2761 aatgccgacg tgtgcgtggc ggcaaacgcc ggttggtgtt ttgaacaaaa agatttgaac
     2821 gctgacaaac tggcagatct gttaactgag ttgttccagc atcccgacga actcagtcgc
     2881 agagcggcca acatgctcac actggcccgg ccccaggcgg ccaaggacat tgtgacagaa
     2941 cttcaatcgt tgtctgtccg gtaatgtttt tgtgatgatt tgactgataa actatgtata
     3001 aaaaacttca ta
//
LOCUS       DATDUN010000125         3652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_462212, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000125 DATDUN010000000
VERSION     DATDUN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462212"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1084)
                     /locus_tag="VJC18_01725"
     CDS             complement(242..1084)
                     /locus_tag="VJC18_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44127.1"
                     /translation="MKNIMIILTMLMAMTAFGCGELDDIESNLKDFNDAFDNATDNST
                     DDSYYDETDQEDHTGEQENDVAEEDYAEESNYNTECDEYGWRYDKTGTYVECDDADSE
                     CEGYEWDPDAYAWYDAQTGLYDFDCDPYDGSSGETGELYDTTGGIVFSPNSKKGTNRY
                     FIGTYVADEWASTCADEAWGPGLDIPYVLRAYSYEDIIDFETSLSQLVWTAIIYPDYT
                     FDFRIKYKDYLGKPSLTLTCTCGFNEMYYNSGMVCACDASNYGACDMYYSPIFSQPLE
                     PYDY"
     gene            1200..1808
                     /locus_tag="VJC18_01730"
     CDS             1200..1808
                     /locus_tag="VJC18_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLD44128.1"
                     /translation="MGGRLEQTLTLVKRAKAGDDQALNLLLSNYMDRVLKMVRLRMGP
                     SIRTRLESMDVVQDVMFRAIKAFDNFEVRNEATFLHWIRKIVQNEINNIADYQFADKR
                     NPNKEMQPVLEEGQNKSIISNIPADSMWSPTGQLQLKDELASLETAMDQLDAEQKEII
                     IMREYEEFSFKEIGQFMSCTPDAARMKFARALDKLTDILNNS"
     gene            1805..>3652
                     /locus_tag="VJC18_01735"
     CDS             1805..>3652
                     /locus_tag="VJC18_01735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLD44129.1"
                     /translation="MTKDINTIFQDYIIKNAEYRPINFDSLIKANPSIEAELNNKIAA
                     YQKIVGVFRGEPAKTCEDPLIGKVINGCLIKATISRGGMSTVYLAHQEALGRDVAVKV
                     LNPVITNSDRSLGRFRRESKNIAKLQHENILPVHDVGEKDGLHFVITDFVEGLSLDKI
                     IEYSIEKGTQPLIKEVATLFNAFGVGEGATIADRFQNYPDFAVNVIKKTALALQYAHQ
                     KGIIHRDIKPSNIIVKPSGEPVLIDFGLSRDFHEMGMTQAGDYLGTPVYSSPEQLFGR
                     QVEVDRQSDIYSLGITFYKLLTGHLPYKGHSLLDTVTNIKRGKQTRPSHYWPEISKYI
                     ERVILKSIQLDKKRRYQDIKEFIDNIDLIISNRPDISKIIRPLGMGQTMKVTGIVLVV
                     IFVVLAFFVGKNFYFDSQVAIEKNLSQYIAYIKSLASHDLPLDGATALMMPDTDLIVY
                     YRINQEKCINECQNSPHDFENRLAKYTVSGIQRIMRQLPDETNLVTYIEYADAVRYYT
                     ATFGMFKDQDLLHAIDDPQTDVIKDQAMRYIVKKDDPVFATMDEWRLGFTKSNDFAKA
                     KRSAGRFTITEFTKYKGFAAPLYLPNILLAVVVDLPDSAKAMQEYLPLNL"
BASE COUNT         1072 a          825 c          789 g          966 t
ORIGIN      
        1 agtaccatcg attgttccat taccacaagt gccggcacca ccaccgcccc ccccatctcc
       61 cggtgtggtg tcgagcgaca cgccagcccc cccgcaaccc atcataaata aatgaattgg
      121 ggctgtgaga aaaatgattc cgagaaacaa aaataaagtg atgattcgat ttctcataaa
      181 agcaggggaa aagggatatc aagtcttgac cccttttcct tttccctttc ccttttctga
      241 cctaataatc atatggttcc aatggttgtg agaaaatcgg tgaataatac atgtcgcacg
      301 cgccgtaatt cgatgcgtca caggcgcaga ccatgccact gttgtaatac atttcgttaa
      361 agccgcaggt gcaggtgagg gtgagcgaag gttttcctaa ataatcttta tacttgattc
      421 taaagtcaaa cgtgtagtcg gggtagatga ttgccgtcca gacgagttga ctcaaacttg
      481 tttcaaaatc tataatgtcc tcgtaggaat aagcgcgcaa gacgtaggga atatcaagtc
      541 caggtcccca ggcttcatct gcgcaggtcg aagcccattc atccgctacg taggttccaa
      601 taaaatagcg gtttgtccct tttttgctgt tcggagaaaa aacaatccca cctgtcgtgt
      661 cgtagagttc gcctgtttct ccagaagagc cgtcataagg atcacaatca aagtcgtaga
      721 gacctgtttg tgcatcatac cacgcatagg catcaggatc ccattcataa ccttcgcact
      781 cagaatcggc atcgtcacat tcaacgtagg tgcctgtttt gtcataacgc cagccatact
      841 catcacactc agtgttataa tttgattcct cagcgtaatc ctcttcggca acatcattct
      901 cttgttcgcc cgtatgatct tcctgatcgg tctcgtcata atatgaatcg tcagttgagt
      961 tatccgtagc gttatcaaaa gcgtcattaa agtcctttaa attgctttcg atatcgtcga
     1021 gttcgccaca gccaaaagca gtcatggcca tgagcattgt taaaattatc atgatgtttt
     1081 tcataagatg ctccttctta agggcacgat tggcccctgt tatgataaaa ggaatacagg
     1141 ttcataaccg aacaaattat atattgagag ctgtctaact atatgcaata gtttgtatta
     1201 tggggggcag actcgaacag accctaaccc ttgtcaaacg tgccaaagca ggagacgatc
     1261 aggccttaaa tctgttgttg tccaactata tggatagggt tcttaagatg gtcagactga
     1321 gaatggggcc ctctatcagg accaggctcg aatccatgga cgttgtgcag gacgtgatgt
     1381 tcagggccat caaggccttc gacaactttg aagtccggaa tgaagcgact tttttgcatt
     1441 ggattcgcaa gattgttcaa aacgagatca acaatatcgc agactatcag ttcgcggaca
     1501 aacgtaaccc caacaaagaa atgcaaccag tattagaaga gggccaaaac aaatccatta
     1561 tcagtaacat cccggctgat tcgatgtggt cacccactgg acaattgcaa ctcaaagatg
     1621 agcttgcctc actcgaaaca gcgatggatc agcttgatgc cgaacaaaaa gaaattatca
     1681 tcatgcgcga atatgaagaa ttttctttta aagagatagg tcagttcatg tcgtgcacac
     1741 cggatgccgc tcgcatgaaa tttgcccggg cattggacaa actgacagat atcttgaata
     1801 attcatgacc aaagacatca acacaatctt tcaagattac attatcaaaa acgcagaata
     1861 tcgtcccata aatttcgact ccttaattaa ggcaaacccc tccatcgaag cagaactaaa
     1921 taacaagata gccgcttacc aaaaaatagt gggggtcttc aggggtgaac cagctaagac
     1981 ttgtgaagat ccattaattg gcaaggtcat taatggctgc ctcatcaaag ccactatcag
     2041 tcgtggagga atgagcacgg tttacctggc ccaccaagag gccttgggca gagacgtggc
     2101 tgttaaggtg ctcaatccag tcattaccaa ttctgacagg tcattgggta ggtttcgcag
     2161 agagagtaaa aatattgcaa agcttcagca cgaaaacatc ttgcccgtgc acgatgtggg
     2221 tgaaaaggat ggccttcatt ttgtgattac cgattttgtg gaaggtcttt ctttagacaa
     2281 aattatcgag tactccattg aaaaaggcac acagccgctg attaaggagg tcgcaacact
     2341 gttcaatgcc tttggtgttg gggaaggtgc cacgattgcc gatcgtttcc agaattatcc
     2401 agactttgct gttaatgtca tcaaaaaaac tgccctggct ttgcaatacg cgcatcaaaa
     2461 gggcatcatc caccgcgata tcaaaccatc aaacattatt gtaaaaccca gtggtgagcc
     2521 tgttctgatt gactttggac tgagtcgcga ttttcatgag atggggatga cacaggcggg
     2581 cgattattta ggaacaccag tttattcctc gcccgaacag ttgtttggta gacaggtcga
     2641 agtagacaga caatcagaca tttattcgct tgggattacc ttttacaaac tgctgactgg
     2701 tcacttgccc tataaagggc acagtctttt ggacacagtg accaacatca agaggggcaa
     2761 acaaactagg cccagtcatt attggccaga aatttcaaag tacatcgagc gtgttatctt
     2821 aaaatccata caactggata aaaaacgacg ctatcaggac attaaggagt tcatcgacaa
     2881 tattgattta atcatcagca accgtccaga catcagcaaa atcatacgcc ctttgggtat
     2941 gggtcaaacc atgaaagtta ctggaattgt cttggtcgtt atttttgttg tactggcctt
     3001 tttcgtgggc aagaattttt attttgatag ccaagtcgca atcgaaaaaa atctttcaca
     3061 atatattgcg tatatcaaat cgttggccag tcacgatttg ccgctcgatg gtgccacggc
     3121 attaatgatg ccagacacag atttaattgt ctactatcgt atcaatcaag aaaaatgcat
     3181 caatgaatgc caaaacagtc cgcacgattt tgaaaatagg ttagccaaat acactgtttc
     3241 gggcattcaa agaatcatgc ggcaactgcc cgacgagacc aatctggtca cctacataga
     3301 gtatgctgac gctgtgcggt attacactgc aacctttggc atgttcaagg accaggatct
     3361 tttgcatgca atcgatgatc cgcagacaga cgttatcaaa gatcaggcta tgcgatacat
     3421 tgttaaaaaa gacgatcccg tgtttgctac aatggacgag tggcggcttg gttttacaaa
     3481 aagcaatgat tttgcaaagg ccaagcggtc tgcgggccgt tttaccatca cagaatttac
     3541 caaatacaaa gggtttgcgg cacctcttta cctgcccaac attctattgg ccgttgtggt
     3601 tgatctgcca gattctgcaa aagccatgca ggagtatttg ccactcaatt tg
//
LOCUS       DATDUN010000126         1316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_462248, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000126 DATDUN010000000
VERSION     DATDUN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462248"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VJC18_01740"
     CDS             complement(<1..227)
                     /locus_tag="VJC18_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD44130.1"
                     /translation="MYAHILCIDDNNEFLFNLRASLKYKYQVSTAINYELAAQVITQN
                     PVDLVFLDVKLGEGESGIRLIQKMKELDPSLV"
     gene            complement(530..>1316)
                     /locus_tag="VJC18_01745"
     CDS             complement(530..>1316)
                     /locus_tag="VJC18_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44131.1"
                     /translation="PGQIFGNGTALSGVTIEVYQNGKYIGSISQVSGIYASGANLVIF
                     KVSGLASGDRLGFVINLSDSSSVSYEGKVSDEQETQAALGVLEADLVPTTELGAVGSL
                     FQEKQTSVGDATAEDKGGIYVVTFGGYEYSADCEIDFVLATDFFMDSTLDADEAAQVL
                     STGSSAFDLTVELAQTDDGYLTWDELSSSGNDYSGVIYQEGSFEFFKGDYVNETNYRY
                     TLIQGTVSDGQIIGDFVSVIVLDGQKLENGLVGSCTVTSSIGN"
BASE COUNT          374 a          360 c          248 g          334 t
ORIGIN      
        1 accaaagacg ggtccaactc cttcatcttc tgaatcagac gaatcccgct ctcgccctcg
       61 cccaatttga cgtcgagaaa aaccagatcc acagggtttt gcgtgatgac ctgagcggcc
      121 agctcataat tgatcgccgt cgaaacctga tatttgtatt tgagtgatgc cctgagattg
      181 aacaagaatt cattgttgtc atcaatgcag agaatatggg cgtacatatc aaaccccttg
      241 tttctcggct atcgaggctg tcgatttagg cgactttctg aaattttgtc atccctgcgc
      301 aggcagggat ccagtaatat caatactttc tggattcctg ctttcgcagg aatgacaatt
      361 catcatacaa attgacagcc tctatccaaa aaaatgattt gctgtccaaa aattgaacac
      421 aacaaagtgt tcagattttg aacgattaat gtcgcttaat taatatctct gtggatttca
      481 agacaataac gcaaaaaata gatgcagtgc attatttgaa agaggtattc taattaccaa
      541 ttgaggaagt cacagtacaa ctgcccacca acccattctc aagtttctgg ccatccagga
      601 caatcacact cacaaaatcc ccaatgatct gaccatcgct cacggtcccc tgaataaggg
      661 tataacggta attggtctca ttgacataat cgcccttgaa aaactcgaac gacccttcct
      721 gatagatcac tcctgaatag tcgttcccag aagaactcag ctcgtcccag gtgaggtacc
      781 catcgtctgt ttgcgcaagt tccacagtga ggtcaaaagc gctactacct gttgagagga
      841 cttgtgccgc ctcatccgcg tcaagcgtgc tgtccataaa aaaatccgtc gccaacacaa
      901 aatctatttc gcaatccgcg gaatattcat aaccaccaaa ggtcaccaca tagatcccgc
      961 ccttatcttc tgctgttgca tcgccaacag aggtttgttt ttcctgaaac agggaaccta
     1021 ccgctcctaa ttccgtcgtc ggcacaaggt ctgcttcaag aacaccaagc gccgcctgcg
     1081 tctcctgttc atccgaaacc tttccctcat aggaaaccga actactgtca gaaagattga
     1141 taacaaaacc aagcctgtcc ccactggcta ggccgctgac cttaaaaatc accagattcg
     1201 ctcccgatgc gtaaatgcca cttacctgcg agatactccc gatgtacttg ccattctggt
     1261 aaacctctat cgtcaccccg ctaagcgcag tcccattacc aaaaatctgt ccggga
//
LOCUS       DATDUN010000127         1362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_255631, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000127 DATDUN010000000
VERSION     DATDUN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255631"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..539
                     /locus_tag="VJC18_01750"
     CDS             318..539
                     /locus_tag="VJC18_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44132.1"
                     /translation="MLDCIMSYANIKIPTVEINLLKNLTKEKTGQKAIMKALAFFFRY
                     AKQRQIADILKNISFDSSFQPLKLRNHER"
     gene            529..918
                     /locus_tag="VJC18_01755"
     CDS             529..918
                     /locus_tag="VJC18_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLD44133.1"
                     /translation="MKDKYILDSSIWIEIERNNEKICGRVLPLIQKNKVCLVDVIVAE
                     VLRGVKTNKDFVTLKDAFSCYTQLSVAWDDVAELAFKLARHGQQPPLLDVYIACATLQ
                     NHKIIVTQDKHFSKIANVVPLQYELLR"
     gene            958..>1362
                     /locus_tag="VJC18_01760"
     CDS             958..>1362
                     /locus_tag="VJC18_01760"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HLD44134.1"
                     /translation="MKKFLTDQQATCPLSIFDPFFLMMSQKNQKFCTTINCMDGRTQE
                     PVIAFLKKHFNATYVDSITEPGPVAILTGPRDTPVFANILKRLDISVYHHKSVGLAIV
                     AHHDCAGNPVTKEQQLKQLAESVKTLQNLYPSI"
BASE COUNT          412 a          341 c          276 g          333 t
ORIGIN      
        1 agggcaagcc ctggacccaa gattccgtgg cgagccacgg ggagtgcatt gcagtagaca
       61 agaatgattt catgtctgag gctgtgcgcc caagccaagt caactcgtgc cccgacctgt
      121 cccccaaagc cttggcgtag gggggaacaa cgtgtagggg tattgaaaca attcatcgta
      181 tgagtgcgaa gccaccagcc ccaaggaaat caaggccaag cacgttgtga caaccattct
      241 gaaaagcagt gcagaatcac cggaatctgc ctctgaatca gtaatgtgta attttcaatt
      301 gtatttttac acttttcatg ttagactgca tcatgagtta cgccaatatc aaaatcccga
      361 cggttgagat caacctgctc aaaaacctca caaaagagaa aaccggtcaa aaggccatca
      421 tgaaggctct ggcttttttc tttcgctacg caaaacaacg tcagattgca gacattctca
      481 aaaacatctc atttgattct tcatttcagc ctctaaaatt gaggaatcat gaaagataaa
      541 tacattcttg actcctctat ctggattgaa atcgaaagaa acaatgaaaa gatttgtggg
      601 agagtgttgc cgttgattca gaaaaacaaa gtttgtctgg tggatgtgat tgtagctgaa
      661 gtgctccgtg gggtaaaaac aaacaaagat tttgtcaccc tcaaggatgc tttttcctgt
      721 tacacacaat tatcggttgc ctgggatgat gttgctgaac tggccttcaa actggctcgg
      781 cacggccaac aaccaccact gcttgatgtc tatatcgcct gtgccacact gcagaaccac
      841 aaaatcatcg tgactcaaga caaacatttt tctaaaatcg ccaatgtcgt tcctcttcag
      901 tatgaactgt tgcgctaaca caaatactgg gatcaaaaat taacgggaaa caagttcatg
      961 aaaaagtttt tgacagatca acaggcaact tgtcccttgt caatctttga ccccttcttt
     1021 ttaatgatgt cacaaaaaaa ccaaaaattt tgcaccacca tcaactgcat ggacggacgc
     1081 acacaggagc ctgtgatcgc gtttctaaaa aaacacttca acgccactta cgtcgattcg
     1141 attacggaac ctggtcccgt ggccattctg acgggccctc gcgacacgcc cgtcttcgca
     1201 aacatcctca aacgtctgga catctcggtc tatcaccaca aatctgttgg actggccatt
     1261 gtcgctcacc acgattgcgc aggcaacccc gtcacaaaag aacagcaact caaacaactt
     1321 gctgaatcag tgaagacact gcaaaacctt taccccagca ta
//
LOCUS       DATDUN010000128         1592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_339991, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000128 DATDUN010000000
VERSION     DATDUN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339991"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /gene="hypD"
                     /locus_tag="VJC18_01765"
     CDS             <1..888
                     /gene="hypD"
                     /locus_tag="VJC18_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HLD44135.1"
                     /translation="TAVSYIDTAIALAKTHGVTLTTFGDMIRVPGSQESLNEIRSQGY
                     PIQVIYSPLDALALAQNNPNKNIVFLAVGFETTTPTIAATILTAHERGVTNFFILPAH
                     KLVMPALTALISDPELKIDGFIMPGHVSTVLGSVPYEFIAQQHRRACVITGFEATDVI
                     LGIHMLIDQIEQNRPCVEIQYNRVVKPQGNPKALGIMNEVFEPQDAIWRGFGVIPQSG
                     MGLRKTFAHFDAYTRFPVTVAPEVENTGCQCGAVLQGKITPRECPLFGRACVPEKPIG
                     PCMISSEGTCAAYYKYRES"
     gene            950..>1592
                     /gene="hypE"
                     /locus_tag="VJC18_01770"
     CDS             950..>1592
                     /gene="hypE"
                     /locus_tag="VJC18_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HLD44136.1"
                     /translation="MNTIQLSHGSGGKKMLELIQNVIFKHLSSPELRQLDDSAELSMK
                     SSRIAFTTDSFVVKPIFFPGGTIGSLAVSGTVNDLLMKGAQPRHLSLGLIIEDGFPLS
                     DLERILGDIAQACQEAHVSIATGDTKVVGRGEADQIFINTSGVGEILPHAHVSGHLAQ
                     NGDVVIVSGTIGEHGVAVMSQRNGLKLTGKIASDAAPLTNTVLPLLEKWGTELH"
BASE COUNT          374 a          467 c          391 g          360 t
ORIGIN      
        1 accgcggttt cctatatcga caccgccatc gcactggcaa agacccatgg ggtcacgctc
       61 acgacttttg gtgacatgat acgtgtgccc ggttctcagg aatccctcaa cgaaatccga
      121 tcgcaaggtt acccgatcca ggtcatttat tcaccgctcg atgccctcgc gctggcacaa
      181 aacaacccca acaaaaacat cgtatttttg gctgttggtt ttgaaaccac cacacccacg
      241 attgccgcca caatcctcac cgcacacgaa cgaggtgtga ctaacttttt tattttaccg
      301 gctcacaagc tggtcatgcc cgcactcacg gccctcatct cggatcctga actcaaaatc
      361 gatggcttca tcatgccggg tcatgtctcc actgtgctgg gctccgtgcc ctacgaattc
      421 attgcgcaac agcaccggcg tgcctgtgtg atcaccggct ttgaggccac cgacgtgatt
      481 ttgggaatcc acatgctgat cgaccagatc gaacaaaatc gaccctgcgt ggaaatccag
      541 tacaaccgcg tggtcaaacc ccagggcaat cccaaggcac tgggcatcat gaatgaagtg
      601 ttcgaacctc aggacgccat ttggcgcggg tttggtgtga ttcctcaaag cgggatgggg
      661 ctcagaaaga cctttgccca ttttgacgcg tacacgcgat ttcctgttac cgtagcacca
      721 gaggtggaaa acacaggttg tcagtgcggt gccgtcctgc agggcaagat caccccgcgg
      781 gaatgtcccc tttttgggcg ggcgtgtgtg cccgaaaaac cgattggtcc gtgcatgatc
      841 tcatcagagg gcacctgcgc ggcgtattac aaatatcgtg aatcgtgaat cgtgaatcgt
      901 gaatcgtcaa tcgatccacg acccacgatc cacgatccac tttttccaag tgaacaccat
      961 tcaactctcc cacggcagtg gcggcaaaaa gatgctcgag ctcatccaga acgtgatttt
     1021 caaacatctg tcctcgccgg aactcagaca actcgatgac tctgctgaac tcagcatgaa
     1081 atcttcccgc attgccttca ccaccgattc gtttgtggtc aaacccattt tttttccggg
     1141 cggcaccata ggctcactcg cggtgagtgg caccgtcaat gatctcctga tgaagggcgc
     1201 acaaccccgc cacctctctc tgggactcat catcgaagat ggctttccct tgtctgacct
     1261 cgaaagaatt ttgggcgaca ttgcccaagc ctgccaagaa gcccatgtca gcatcgcgac
     1321 gggtgacacc aaagtggtgg gccgcggcga ggctgaccag attttcatta acacatctgg
     1381 tgtcggcgaa atcctgcccc acgctcacgt gagtggtcat ctggctcaaa atggcgatgt
     1441 ggtgattgta tcgggaacca ttggtgagca tggtgtggcc gtcatgagtc aaagaaacgg
     1501 cctcaaactc accggcaaaa tcgcgtcaga cgcggctcct ttgaccaaca cggttctgcc
     1561 gcttttggaa aaatggggaa ccgaacttca tg
//
LOCUS       DATDUN010000129         3749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_90556, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000129 DATDUN010000000
VERSION     DATDUN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90556"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(549..947)
                     /locus_tag="VJC18_01775"
     CDS             complement(549..947)
                     /locus_tag="VJC18_01775"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLD44137.1"
                     /translation="MENEHDKLINKAERSLNAGERLYHEGDHDFAASRIYYALFYLAE
                     ALLLEKGLSFSRHSAVIAGFYEHFVKTNIFPKEFHKLLHHAYNLRQDGDYQGDCVVEE
                     AEVSDLIAKAKKFFLAVKAQITKSATKKLF"
     gene            complement(937..1461)
                     /locus_tag="VJC18_01780"
     CDS             complement(937..1461)
                     /locus_tag="VJC18_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLD44138.1"
                     /translation="MNTLLTIPELSKKIKLSEIAIYKMLKRKSIPGIRVGKLWRFDEE
                     EIQRWLKDHSSLPEQSTTQKPPHIEKLLNHLNQHLKKQFGKKYLRLVLYGSWARQTAT
                     EQSDVDALVIVSDIKNPSQIRQKVHQIAYEVSEKFQHKHLIAITLMTENEYLTGSSAL
                     LMNIRKEGRVIHGK"
     gene            1611..3308
                     /locus_tag="VJC18_01785"
     CDS             1611..3308
                     /locus_tag="VJC18_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44139.1"
                     /translation="MKDTMISPDDPTHFTLPFSLELDSPDQQNLEQIPGDQYVALNTQ
                     LDLVYHEEIYNAVSQEFDPFTLLTREQPSLRPCEIDGRISDGTERTLLVICLDIIADY
                     IDKERTLLSSEEKILFSKLHDEEIPWTQKEPLLISRQALVDQKEELTRFTQSVVAFRQ
                     AVGASKDIAISYASSGLSRDAPPVIEAQQQQELILAIEKTLPSVQGYKKTCLLYVHKE
                     LDASISYELFNITTKESYADLTVDDEFLSFISKQTVLSPHFVGKLKATPVDYMPSQKA
                     QTPTLIVYLDHHLRLPVQKAIYADMVAFASLNPVFGFEDPQASQSYEVRFDILSTNAS
                     QRVQDLAFGKEYRAQQNTANLLHRQIRAFDSAFEKMYAQKLLYENSAQPSSHDTGDTG
                     KQGLFEAKDYNPTLRRLQMRYELTDRRFSDAIDKATMISFAASPIFACSELSFEESIA
                     LISQNTDLLLGDNNYLGGLLELCLYEGGIYALGDAYPTIDIDTTGSYLLDDLIVMHRS
                     RLAVDDVLSKMRTQQKPLGVMVMGRGHYQSIRSYLEQKGNVNVIFMAPTEYNGPMPE"
     gene            complement(3361..>3749)
                     /locus_tag="VJC18_01790"
     CDS             complement(3361..>3749)
                     /locus_tag="VJC18_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44140.1"
                     /translation="SAITQTFEAIQIRDQEYTYIERMRVNATHLWYQSWDEASVPQTP
                     DDVADVKDTPSDNRCLEIGDSIVASRYVPFEDCVTAFGAADVNDLNGDENYTLKMIDI
                     QTAGSIAFGTPITDTPETSCLEENAQ"
BASE COUNT         1001 a          882 c          834 g         1032 t
ORIGIN      
        1 acaggccaaa atttattgaa gtggtagaga cagaaatcat cagtcttcac gagggcaata
       61 ttgcccgcta tcatctacga ccctcgcaat atcaggcttg ggaaaaaacg tggagatgaa
      121 cccaaattgt aacatcacct ctgcgtattt ttacaccgcc gtaaaatccg tctgacagtg
      181 actaaatgga ttttgggagg ttgttgcaca atagcctgaa atggctacag gattattttt
      241 tgtactgttg gcacaggcat tgctactatg tgaggtgtcc aaattatttt tggatgacgc
      301 catacacccc gaaggggact ggtcgtttac aaaaaattcg atcagtctcc tgtttttgtc
      361 ctgccccata ctgtcattga acacaagccg acaatcatca cgttcattgc gccgttggct
      421 tttctgctgt ccacgaaaaa aaaagttagt gctcgagatg acacgcgttg tcaaaacggc
      481 gaagaaagaa tagggtatgc cttctaaggt tgtacagctt gttccgcaac agaggcacac
      541 actacctgtt aaaataattt tttggtagcg cttttggtga tttgcgcttt gacggctaaa
      601 aagaattttt ttgccttggc aataagatca ctgacttcgg cttcctcaac cacacagtca
      661 ccttgataat cgccatcctg tctcagattg tacgcatgat gcaggagctt gtgaaactcc
      721 ttgggaaaga tgtttgtttt cacaaaatgt tcgtaaaaac ccgcgatgac ggcagagtgc
      781 cttgagaagc tcagtccttt ttccaacagt aatgcctctg ccaaataaaa aagtgcatag
      841 taaatccgcg atgccgcaaa gtcgtgatca ccttcgtggt atagtcgctc tcccgcattc
      901 aatgagcgtt ctgccttgtt gatcagtttg tcatgttcat tttccatgaa tgaccctgcc
      961 ttccttcctg atgttcatga gaagtgctga tgacccagtc agatactcgt tctcagtcat
     1021 gagtgttatg gcaatcaggt gtttgtgttg gaatttctcg cttacctcgt aagcaatttg
     1081 gtgcaccttt tgcctgattt gcgaggggtt cttgatgtcg gagacaatga ccagcgcgtc
     1141 aacatcggat tgttcagtag cggtttgtct tgcccaagac ccataaagaa ccagtcgtaa
     1201 atatttcttg ccaaattgtt tcttcaggtg ctggttcaga tggttgagca acttttcgat
     1261 gtgagggggc ttttgtgttg tggattgttc tggtaacgaa gagtggtctt ttaaccagcg
     1321 ctggatttcc tcctcatcaa accgccagag tttgcccact ctgatgcccg ggatgctttt
     1381 gcgtttgagc attttgtaga tggcaatttc gctgagtttg attttctttg acagctcagg
     1441 aatggtgaga agtgtgttca tataatataa ccaaatatag gcatatatta ttatatatta
     1501 ataaatataa aagatcaatt gtgaatacat gtgtccatca ggacacgaaa tttggatgtt
     1561 gcgcgcaact tgcgagcgaa tcgcggcgat agtgcctaaa aggaagtgcc atgaaagaca
     1621 ctatgatttc gccagacgat cccacccatt ttactttgcc cttctcactg gaattggatt
     1681 ccccagacca acagaatttg gaacaaattc ccggcgatca atatgtggca ctcaacactc
     1741 agctagacct cgtttatcat gaagaaatct ataatgccgt ttcgcaggaa tttgatccct
     1801 tcaccctcct gaccagagag caaccatccc tgagaccctg cgagattgac ggtcgtatct
     1861 ccgatgggac agaacgcacc ctcttggtaa tttgcctgga tatcatagct gattatatag
     1921 acaaagagcg cactttgctg tcatcggaag agaaaatact tttttcaaaa ttacacgatg
     1981 aggaaatacc ctggactcaa aaagagcctc ttctgatatc gagacaggca ctggtggacc
     2041 aaaaagaaga actcacacga ttcacgcaga gcgttgttgc cttcaggcag gctgttggtg
     2101 catcaaaaga tatagccatc tcatatgcca gttccggtct ttcaagagac gccccacctg
     2161 tcattgaggc ccaacaacaa caagagctga tcctggccat tgaaaaaacg ttgccatcag
     2221 tgcaaggcta caaaaaaaca tgtctgctgt atgttcataa agaacttgat gccagtattt
     2281 cttatgaatt gtttaacatc actactaagg aatcatatgc agacttaacc gttgatgatg
     2341 aattcttgag ttttatttca aagcagacag ttctttcacc ccattttgtt ggaaaactca
     2401 aggcaactcc tgttgactac atgccgtccc agaaagctca aacaccaacg ctaatcgtgt
     2461 atctggatca tcacctacgc cttcccgtgc aaaaggctat ctatgcggat atggtcgcct
     2521 tcgcgtcgct caatcctgtc ttcggtttcg aagatccaca agcaagccaa tcgtatgagg
     2581 ttcgctttga tatattatcg acaaacgcat cgcaacgcgt tcaagattta gccttcggaa
     2641 aagaatatcg tgcgcaacaa aacacggcta atctactaca tagacaaatt agggccttcg
     2701 atagtgcatt cgaaaaaatg tatgcccaaa aacttctata tgagaactca gcccaacctt
     2761 cttcgcatga tactggtgat acgggtaagc agggcctctt tgaggccaag gattacaatc
     2821 cgactctacg taggttgcaa atgcggtacg aacttactga tcgaagattt tctgatgcaa
     2881 tagataaagc aacaatgatc tcattcgctg cttcacccat ctttgcttgt tcggagcttt
     2941 cgtttgagga aagcattgcc ctcatttccc aaaacaccga tctgttactt ggagacaaca
     3001 attatttggg cggcctgctc gaattgtgtc tctacgaagg cggtatctac gccttggggg
     3061 atgcgtatcc cacgattgat atcgacacaa caggctcgta tcttcttgat gacctgattg
     3121 ttatgcaccg atcacgcctg gctgtggacg atgtcctctc caagatgcga acgcaacaaa
     3181 agccattggg tgtgatggtg atgggcagag gtcattacca atccatcaga tcctatctgg
     3241 aacaaaaggg caatgtgaac gtcatcttca tggcccccac ggaatacaac ggccccatgc
     3301 cggaataggg gcgggcaggt ttggaacctg ccaacattcg gacaggtttg gaacctgccc
     3361 ctactgggca ttttcttcca aacacgatgt ttctggcgtg tctgtgatcg gtgtgccgaa
     3421 ggcaatggaa cccgccgtct gaatgtcgat catctttaac gtgtaattct catccccgtt
     3481 aagatcgttc acgtctgcgg caccaaacgc cgtcacacaa tcctcaaacg gcacgtaacg
     3541 tgaggcaaca atgctgtcgc caatttcgag acaccgattg tctgagggcg tgtccttcac
     3601 atctgccacg tcatcgggag tttgcggtac cgaggcctca tcccacgact gataccacaa
     3661 atgcgtggcg ttgacgcgca tgcgctcaat gtatgtgtat tcctgatcgc gaatctgaat
     3721 ggcctcaaat gtttgtgtga tggccgaca
//
LOCUS       DATDUN010000130         1995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_131581, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000130 DATDUN010000000
VERSION     DATDUN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131581"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VJC18_01795"
     CDS             <1..250
                     /locus_tag="VJC18_01795"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HLD44141.1"
                     /translation="LFVMGLCITLIPMLVTTLVARLIFKINFLTILGLLTGGMTSSPG
                     LSTVDAITESNAPSVAYALVYPVAMVLMVVFAQLLCHW"
     gene            330..1535
                     /gene="pgsB"
                     /locus_tag="VJC18_01800"
     CDS             330..1535
                     /gene="pgsB"
                     /locus_tag="VJC18_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HLD44142.1"
                     /translation="MIIASKYFFILLVTTMALVAVGVVHFLRHQKKLLSIPIRIHVNG
                     TRGKSSVTRLIAAGLKAGGIRTCAKTTGTLPRMIMPDGREYPIFRPGRANVAEQVRVV
                     SAAYARQVEALVVECMALLPYLQWLSEAKMIRATHGVITNARADHLDVMGPDEEDVAW
                     ALCGMVPQKGKLFTCEQKHLPLIQQVCQDRGAECIALSAEDIKQISSEELAAFSYIEH
                     PDNVALALKVCAAVGVKREVALQGMYQAVPDVGALRDFEINFFGRKMFFVNGFAANDP
                     ESTEKVWNIALSRYPNVKKKIAIFNCRVDRPERSVQLAEAFTRWQCADHLIIMGTGTY
                     WFSKELVPSGFDYHSITYADNQSVEEIFELVVSLAGSSSVVVGMGNIGGQGLDLVTYF
                     KNRAVVRED"
     gene            1575..>1995
                     /gene="pgsC"
                     /locus_tag="VJC18_01805"
     CDS             1575..>1995
                     /gene="pgsC"
                     /locus_tag="VJC18_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HLD44143.1"
                     /translation="MTGILTLSIGLGLAIGLTFAELFGIAAGGMVVPGYIALNLSHPL
                     DVAMTIFTAMLTYWVVKSLSPFVFLYGRRRTALMILMGYLVGIGIHYFFPTTALLFGI
                     DFQVIGFIIPGLLAIWMDRQGFAETISMLLIVAVIIRL"
BASE COUNT          417 a          478 c          584 g          516 t
ORIGIN      
        1 gttatttgtc atgggtttgt gcatcacact catccccatg ctggtgacga cgcttgtggc
       61 gcgattgatc ttcaagatca attttctgac catattgggc ctgctcacag gcgggatgac
      121 cagttctccc gggctcagca cggtggatgc gatcacggag tctaacgctc cgagtgtggc
      181 ttacgcgctg gtgtatccgg tggcgatggt gcttatggtc gtgtttgcgc aattgttgtg
      241 tcactggtaa gttaaaccgt tgaaatcgcc aaatctcggc gttgctcatg aagaatgaaa
      301 tcgccaaatc ttgatagaag gcaacaccca tgatcattgc atcgaaatat ttttttattt
      361 tactcgtgac cacaatggcc ctggtggcgg ttggcgttgt gcattttctg cggcatcaaa
      421 aaaagttgtt gtccatcccc atccgcattc atgtgaacgg gacccggggc aaatccagtg
      481 tgacccgcct catcgccgcg gggctcaagg ccggagggat caggacctgc gcaaaaacaa
      541 cggggacctt gccccgaatg atcatgcctg atggccgcga ataccccatt ttcagacccg
      601 ggcgcgccaa cgttgccgaa caggttcgtg tggtgagcgc cgcgtatgcg cgccaagtgg
      661 aggccctggt ggtggaatgc atggccttgt tgccttacct gcaatggctg agcgaggcca
      721 aaatgatccg ggccactcac ggggtcatca ccaacgccag agcggatcac ctcgacgtga
      781 tgggtcccga cgaagaagat gtggcttggg cactctgtgg catggtccct caaaaaggca
      841 aattgttcac ctgtgagcag aagcacttgc cccttatcca acaggtctgc caggaccgcg
      901 gggcggaatg tattgcgctg agtgcagaag acatcaaaca aatcagctct gaggagctgg
      961 cggccttttc gtacattgaa catcccgata atgtggcttt ggcactcaag gtctgtgcgg
     1021 cagttggggt caagcgcgaa gtggccctgc agggaatgta tcaggccgtt cccgacgtgg
     1081 gggccctcag ggatttcgag atcaattttt ttggacgaaa aatgtttttt gtgaacgggt
     1141 ttgcggccaa cgatcctgaa tcaacggaaa aggtgtggaa tattgctttg agccgttatc
     1201 ccaacgtcaa aaagaaaatt gccattttca attgtcgcgt cgacaggccg gagcgatctg
     1261 tgcagttggc ggaggccttc acacgatggc aatgtgccga tcacctgatc atcatgggaa
     1321 ccggaacgta ctggttttcc aaggaactgg tcccgtcagg ttttgattat catagcatca
     1381 cctatgctga taatcagtct gtggaagaaa tatttgaact ggtggtgagc ttggcagggt
     1441 cttcttccgt ggtcgtgggg atgggcaata tcggtgggca ggggctggac ctggtgacgt
     1501 attttaagaa ccgcgctgtg gtgcgggagg attaaataaa tgaaatggca ggctagaggc
     1561 gaggggaggt ttgagtgaca ggaattttaa cattgtccat tggtttgggt ctggcgatcg
     1621 gactcacgtt tgcggagctc tttggcatcg ccgccggcgg gatggtggtt cccggttaca
     1681 tcgcgctcaa tttgtcgcac ccgctcgatg tggccatgac tatcttcacg gccatgctca
     1741 cctactgggt tgtgaagtct ctgtccccct ttgttttttt gtatggccgc aggcgaacgg
     1801 ccctgatgat tctgatgggc tatctggtcg gcattggcat tcattatttc ttccccacaa
     1861 cggcgttgct gttcggtatt gattttcagg tcattgggtt catcattccc ggactcttgg
     1921 ccatctggat ggatcggcag ggatttgccg aaacgatttc catgctgttg attgtcgcgg
     1981 tcatcattcg gctgg
//
LOCUS       DATDUN010000131         3217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_171946, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000131 DATDUN010000000
VERSION     DATDUN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171946"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1331)
                     /locus_tag="VJC18_01810"
     CDS             complement(33..1331)
                     /locus_tag="VJC18_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HLD44144.1"
                     /translation="MAKISGKVSVATVLLWGEEVGAVAWDDERGLGRFEYADRFLQQG
                     FELAPLMMPRRSGVYAFPELNRKTFQGLPGLLADALPDRFGNQLIDLWLKQNGRSLDD
                     FSPVERLCYMGTRGLGALEFKPAFAVAGRAVPVEVPELTKLANQILNDRQHLKVKLTG
                     KKAEALNHIIRVGTSAGGNRAKAVIAWNPKTNEVRSGQVMVPEGFEPWILKFDGVNNA
                     SLGDPSGFGRIEFVYHQMARKAGLEMAPCRLMEENGRAHFMTRRFDRDADNQKIHMQS
                     LCALAHFDFNAAGEYAYEQVFDVIHKLNLGHDIMREMFRRMLFNVVARNQDDHTRNTA
                     FLMDRNGVWRLAPVFDVVWSFNPHGKWTNQHQLTINGKRDDFVRADLVSVAKLYSIKN
                     VSEIFDQVAAAIKGWPRLAKAVGVSQKMIALISKTHRVLI"
     gene            complement(1335..1658)
                     /locus_tag="VJC18_01815"
     CDS             complement(1335..1658)
                     /locus_tag="VJC18_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006661622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLD44145.1"
                     /translation="MDFLAASDRVVMQALGERIHRHRLNRNVTQASLALKAGVARIVI
                     HRLENGLGCTLESFIRILRALGAIAQLDLFLPDPGISPRQLAKLKGHQRQRASGQRAS
                     NQKGR"
     gene            complement(1794..2774)
                     /locus_tag="VJC18_01820"
     CDS             complement(1794..2774)
                     /locus_tag="VJC18_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLD44146.1"
                     /translation="MARPLRIEYEGAWYHVINRGQTRRNIVRTDEDRKLFLKLLGDTQ
                     QTYGIEIHAFSLMDNHYHLLVHTPKAGLSRAMRHLNGVYTLKINQIWNTDGPIFKGRY
                     NARLVDSEEYLLELIRYIHLNPVVAKLCAKAQDHQWTSHGYYIDRKRRPTWLSTQQIL
                     SRFGGAERTALERLDKFVHEGVPVWFSEEIKRNRIVLGRECFRDWVYHNYVDPDRDDI
                     LLGEKKPWQRISIKKILEKVSFVYDVPISEIRKARQGKQNEARTVAVYLARQISGARQ
                     VDLVKWFNATNRYAIAKMIQRFKEVQAKDKNLNRIMNGIKSHLYSQRQNQ"
     gene            complement(2832..>3217)
                     /locus_tag="VJC18_01825"
     CDS             complement(2832..>3217)
                     /locus_tag="VJC18_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44147.1"
                     /translation="GANELCDGEDNNCDKTVDEGCPVAPAEDPAVPAPDVPESADAEK
                     TEEIVGDEESPDVVESPTDDDVPEDETDEDTENDENGDDTFEPSDDNKFPNPAVDDAD
                     DELDGDSKETEATDDQGGGGCSLRI"
BASE COUNT          791 a          887 c          638 g          901 t
ORIGIN      
        1 cacctaaaaa acaaataggg gaattgaaca cgttaaatca gcacgcgatg agttttggaa
       61 atgagcgcaa tcattttttg cgaaacacca accgccttcg ccaaacgagg ccagcctttg
      121 atggcggcag caacttgatc aaagatttct gaaacgtttt tgatactgta aagttttgca
      181 acgctcacca aatccgccct cacgaaatcg tcgcgtttac cattgatggt cagctgatgc
      241 tgattggtcc atttcccgtg aggattgaac gaccagacca catcaaagac cggagcgagt
      301 ctccagacac cgttccgatc cattaaaaat gccgtgttac gtgtgtgatc gtcttgatta
      361 cgcgctacga cgttgaaaag catgcgtcga aacatttcgc gcatgatgtc atgccccaaa
      421 ttcaatttgt ggatcacatc aaaaacctgt tcatacgcat actctccggc agcgttaaaa
      481 tcaaaatgcg ccaatgcaca caatgattgc atgtgaatct tttgattgtc tgcatcgcga
      541 tcgaaacgcc gcgtcataaa atgggctcgg ccgttctctt ccataaggcg gcaaggcgcc
      601 atctccaggc ccgcttttct ggccatttga tgatagacaa actcaatcct gccaaaacct
      661 gaggggtcgc caagtgatgc attgttcaca ccatcaaatt tcaaaatcca cggttcaaaa
      721 ccttctggca ccatcacttg tcccgaacgc acttcgttcg ttttgggatt ccaagcgatc
      781 accgctttgg cgcgattgcc tccagcggac gttcccaccc gaatgatgtg attgagcgcc
      841 tctgcttttt tgcccgtcag tttcactttc aaatgctggc ggtcgttcag aatttgattg
      901 gccagtttgg tcaattcagg cacttctaca ggaacagcgc gcccggcaac ggcaaacgct
      961 ggtttgaatt ctaaggcccc caggcctcgc gtgcccatgt aacacaatct ttccaccggc
     1021 gaaaaatcat ccaaagacct tccgttctgc ttcagccaca aatcaatcaa ctgattgcca
     1081 aagcggtctg gcagggcatc tgccaacagt cccggcaaac cctgaaatgt ttttctgttc
     1141 aattcgggaa aagcataaac accagatctc cgaggcatca tcagtggagc caattcaaat
     1201 ccttgttgca aaaaccgatc agcatattca aaacgcccaa gaccgcgttc atcatcccaa
     1261 gcgacggcac caacttcttc tccccaaaga agaacggtag ccacactgac tttgcctgaa
     1321 attttcgcca tggatcacct gcccttttga ttgctcgcgc gctgacccga tgccctttgt
     1381 ctttgatgac ccttcagctt tgccagttgc ctggggctaa tccccggatc cggcagaaac
     1441 aaatcgagct gggcaatcgc tcccaaagcc cgcaaaatgc gtatgaaact ttccaacgtg
     1501 cagcccaaac cattttccaa acggtggatc acgatgcggg ccacacctgc tttcaacgcc
     1561 aaacttgctt gcgtgacatt ccgattgagt ctgtggcgat ggatgcgctc tcctagcgct
     1621 tgcatcacca cccgatcgct cgctgccaaa aaatccataa tatttccttt ttttgtgcct
     1681 tatatataat acctaattat gtaataattg caacaatctt ttatttatag tacacaatga
     1741 ttgattcaaa caatggggtc aacaatgggg tcaaagattg acaagagaca agttcattga
     1801 ttttgacgct gtgaataaag gtgtgatttg attccattca ttatgcgatt cagattctta
     1861 tcttttgctt gcacctcttt aaatcgttga atcatttttg cgatcgcgta tctgttggtc
     1921 gcattaaacc attttaccaa atccacctgt cgtgccccag atatttgcct cgctaaatat
     1981 accgccacgg tcctcgcctc attttgtttg ccctgccgcg cctttctgat ctcagaaatg
     2041 ggcacgtcgt agacaaaact aactttttct aatatttttt ttattgaaat cctctgccac
     2101 ggttttttct cgccaagcag aatatcatct ctatcaggat ccacatagtt atgatacacc
     2161 cagtccctaa agcattctct ccccaacaca atacggtttc tttttatttc ttcgctgaac
     2221 cacaccggca caccttcatg gacaaattta tccagacgct ccagggctgt cctttctgcg
     2281 cccccaaacc gactcaatat ttgttgggtc gacaaccacg ttggtcgtct cttcctgtcg
     2341 atataatagc catggctggt ccactgatgg tcctgtgctt ttgcacacag tttggcgacg
     2401 accggattca aatgaatgta ccttatcagc tcaagaagat attcttcgct gtctacgagc
     2461 cgcgcgttgt atcttccctt aaaaatcgga ccatccgtgt tccagatctg attgattttt
     2521 aatgtataaa cgccgtttaa atggcgcatc gcccttgata aacccgcctt gggcgtgtgg
     2581 accagcaaat gatagtggtt gtccattagg gaaaatgcat gaatctctat gccatatgtt
     2641 tgttgcgtgt cgcccaatag cttaagaaat aactttcgat cttcatctgt tctgacaatg
     2701 ttacgccgtg tctgccctcg attgatcaca tgataccagg ctccttcata ttctatgcgc
     2761 aatggtcgtg ccatacccat ctcatatgat cacacttgtc tcttgtcaat ctttgacccc
     2821 tcggctgtgc tctaaattct cagcgaacac ccaccgccgc cctgatcatc tgttgcctcc
     2881 gtctctttgg agtcaccgtc aagctcatca tcagcatcat cgacagctgg gttcggaaat
     2941 ttgttgtcat cacttggctc aaaagtatcg tctccatttt catcgttttc cgtgtcctca
     3001 tctgtttcat cttcaggcac atcgtcatcg gttggtgatt caacaacgtc aggagattcc
     3061 tcgtcgccaa caatctcttc agttttttcc gcgtctgctg attcgggaac atctggggca
     3121 ggaaccgcag gatcctcagc tggtgccaca ggacaaccct cgtccacggt tttgtcacag
     3181 ttattgtcct ccccatcaca cagctcattc gcccccg
//
LOCUS       DATDUN010000132         1221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_545076, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000132 DATDUN010000000
VERSION     DATDUN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545076"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /gene="gspN"
                     /locus_tag="VJC18_01830"
     CDS             complement(<1..449)
                     /gene="gspN"
                     /locus_tag="VJC18_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HLD44148.1"
                     /translation="MNRTVQIIVYVFTLVVAFVGFVVLLFPLDTVIGHFLAQVEEDTA
                     GKYRVVVADIDGSLIFDSHFKNFRVYQQGREILSVPELDVGFSLFSLLGDNKTVGFDA
                     VFEKGEVSGKVALSTKGKQIGLKMIDVGFENFATDQLRFIEDYLASA"
     gene            complement(462..1049)
                     /locus_tag="VJC18_01835"
     CDS             complement(462..1049)
                     /locus_tag="VJC18_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44149.1"
                     /translation="MINWDNIKSQLNVEELYNKFLWLDQKQQYFVVLALAAVLIVILI
                     LPVNWISSQLNSREAEYREVIKTASELYDKLTQYNGLKNSVGKSANASAGVEIDIKKI
                     VIQMTDTIGLDRHKATLKTIPDVPKGDFIEEGKEITLRNIDFEQTVQLLDQIISYSEV
                     PLRVNKLTMQADRRNKQQMNTVVMTVSVLKPVTTE"
BASE COUNT          320 a          326 c          242 g          333 t
ORIGIN      
        1 ttcgccgatg ccaggtagtc ttctataaac ctgagctgat cagtggcaaa attttcgaac
       61 cccacatcaa tcatcttgag cccaatttgt tttcctttgg tgctgagagc gactttgccc
      121 gaaacttccc ctttttcaaa aacagcgtca aaccccaccg tcttgttgtc tcccagcaaa
      181 gaaaacagcg aaaaacccac atccagttcc ggcacactaa gaatttcgcg cccttgttgg
      241 tacacacgaa aatttttaaa atgggaatca aaaatcaaac tgccatcaat gtcggccacc
      301 acaacccgat acttccctgc ggtatcttct tcgacctggg cgaggaaatg tcctatcact
      361 gtatcgagag gaaacaacaa gaccacaaag cccacaaagg ccacaaccag ggtgaaaacg
      421 tacacaatta tttgaacggt ccgattcaaa tcaacactcc ctcattctgt ggtaaccggt
      481 tttagcacgc tcaccgtcat caccaccgta ttcatttgct gtttgttgcg acgatccgcc
      541 tgcatggtca gcttattgac cctgagaggg acttcgctat agctgatgat ctgatcgagc
      601 aactgaaccg tttgctcaaa atcaatgttg cgcaaggtga tctcttttcc ctcttcaata
      661 aaatctccct tgggcacatc agggattgtt ttcaacgtgg ccttgtggcg atccaaacca
      721 atggtatcgg tcatctggat tacgattttc ttgatgtcta tttcgacccc ggcagacgca
      781 ttcgccgatt tgccaacact atttttcagg ccgttgtatt gggtgagttt gtcataaagc
      841 tcggaagccg ttttgatcac ctcgcggtat tcagcctccc gtgaattgag ctgtgacgaa
      901 atccagttga cgggcaaaat aaggatgaca atcaacacag ccgcaagcgc caacacaaca
      961 aaatattgct gtttctggtc caaccacaaa aatttgttgt acagctcttc aacattgagc
     1021 tgtgatttta tgttatccca attgatcata cgtcattccg aactcgacac cgattttttg
     1081 aggtcgatct ttaaatcgaa atcccactgt cccccgggtt taggctgcgc atccgacgcc
     1141 acaatgctga caaacttgtc tgatttcttc agggcaccca caattttttc gacgttcagg
     1201 gtgtcgttcg tcgtcgcatc a
//
LOCUS       DATDUN010000133         1434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_421669, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000133 DATDUN010000000
VERSION     DATDUN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421669"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VJC18_01840"
     CDS             complement(<1..191)
                     /locus_tag="VJC18_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44150.1"
                     /translation="MPQNLSKKVVRTIQNLDPHDELLLSAISPWEFSKLIEKKRLRIT
                     ISPEEWIDRALDIPHLRLVP"
     gene            complement(233..460)
                     /locus_tag="VJC18_01845"
     CDS             complement(233..460)
                     /locus_tag="VJC18_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLD44151.1"
                     /translation="MAISLFKANALRLIEQVARFKESLIITKRGKALAEIVPIHSSNL
                     KPRPGLLVDTLDKAGDLISPVSDASAWEACK"
     gene            571..1317
                     /locus_tag="VJC18_01850"
     CDS             571..1317
                     /locus_tag="VJC18_01850"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLD44152.1"
                     /translation="MDKNADKTLTRESLLTHLKFMQEIGLEEVMPSLSIHPSCETASD
                     KPIPETVTKATDRHRQELPTEVKSLQELQETLTGCTRCPLHAGRTKIVFGQGNPHADL
                     MFVGEGPGADEDKQGLAFVGRAGQLLTDMIKAMKLSRDAVYIANIVKCRPPGNRAPEP
                     NEMSACMPFLKQQIYLVNPKVIVCLGKTAAQALLQTETPISKIRGVFGKFEGIPLMPT
                     FHPAYLLRNPAMKKPVWEDLQKVMRVLELE"
BASE COUNT          374 a          362 c          353 g          345 t
ORIGIN      
        1 ggaaccagac gcaaatgagg aatatcgagg gcgcggtcaa tccattcttc aggtgatatt
       61 gtgatcctca gtcttttttt ctcgatgagc ttcgaaaact cccagggaga tatggccgaa
      121 aggagcagtt catcatgtgg atccaaattc tgaattgttc tcacaacttt tttcgataga
      181 ttctgcggca tcatatgcca ccaaatccaa acatgtgtgt caaaaaggta attcatttgc
      241 acgcttccca agccgaagca tcgctgaccg ggcttatcag gtcacccgcc ttgtcgaggg
      301 tgtctaccaa caagcccggc ctaggtttaa ggttcgacga gtgaatggga acaatttcgg
      361 ccaacgcctt gcctcgcttg gtgatgatga gactttcctt gaacctcgcg acctgctcaa
      421 tgagcctcag ggcgttggcc ttgaacagac tgattgccat tgtttgagcc atgcctcatg
      481 ataacatggt catttgaaat ggtcaagtct gtttcgactc gtatcatgtt ccaggtttac
      541 aatggttcaa aaaaaaacta ttgtgcgatt atggacaaaa acgccgataa aacactcacc
      601 cgagagtcac tgcttacaca cctcaaattc atgcaagaaa ttgggcttga agaggtcatg
      661 ccgtcgctat caatacatcc ttcttgcgaa acggcgagtg acaaacccat ccctgagact
      721 gtcaccaaag caacagacag acacagacaa gaactaccaa ctgaagtaaa aagtctgcag
      781 gaacttcaag agaccttgac cggttgtaca cgctgtcccc tgcacgcggg ccgcacgaag
      841 atcgtttttg gccaaggcaa cccccatgct gatttgatgt ttgtgggcga aggccctgga
      901 gccgatgaag acaagcaggg tctggccttt gtgggcaggg ccgggcaact gctgactgac
      961 atgatcaagg ccatgaagct ctcgcgcgat gctgtgtaca ttgccaacat tgtcaaatgc
     1021 cgcccgccgg gcaaccgcgc accagaacca aatgagatgt ctgcgtgcat gcctttttta
     1081 aaacagcaga tttatttggt gaaccccaag gtcattgtgt gtttgggcaa aaccgccgcg
     1141 caggccctct tgcaaacaga aacacccatt tcaaaaatca ggggcgtgtt tggaaaattt
     1201 gaaggcatcc cgctcatgcc cacgtttcac ccggcctatc ttttgcgcaa ccccgccatg
     1261 aaaaaacctg tgtgggaaga tttgcagaag gtgatgaggg tgttggagct tgagtgatca
     1321 ctccccggtt tttctggcca gaggaatttg attgacgctc agattggcgt acacgccgcc
     1381 atagtgtccc agcaattttg tgccgggcga atactggtcc gcgtagtcat agaa
//
LOCUS       DATDUN010000134         1254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_213290, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000134 DATDUN010000000
VERSION     DATDUN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213290"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1254
                     /locus_tag="VJC18_01855"
     CDS             <1..>1254
                     /locus_tag="VJC18_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44153.1"
                     /translation="ESESSGADTFVFFDGFDAASIDTSKWTQANVLDDFNVVDGKLVL
                     GAGTSSFNQALYTTSAFARSNLVMEFDYTWTSANTSYDAIMMGWKDDGTGRTQTNLVY
                     AHYNAGASTCTVNCTGYVFEDGTSRSGVTSKYTQNQPYRLRLSMKTEGGATYQQSSDA
                     GQTWSTVYNSVHSTESNLHPGFVLHSGTHVIDNVRVRPWTSTEPTVSIDAEVSQYSAV
                     SGTLLSSVYDTGEAGCAVESIAHTVSDTGLVEIRLRSANAPNMSDATDFSACDAIDGS
                     TSFTAYPCVQAHDRYLQYQLVLGTEEDLGPTLTDITINYDSLSLQANAGEDRIVAAGR
                     AITLDGLASTGIDLSYAWAVFEGEGVLADEFTATPTLTIPAGAQRSQTSVTLTVYDAY
                     GQHASDEVTLSILGTPTLSLAENKLA"
BASE COUNT          307 a          372 c          308 g          267 t
ORIGIN      
        1 agaatccgaa agtagcgggg ctgacacatt tgtatttttt gatggtttcg acgccgcctc
       61 aatcgatacc tcaaaatgga cacaagccaa tgttctggat gattttaacg tggtcgatgg
      121 aaaactagtc ttgggggcag ggaccagcag ttttaatcag gccctgtaca caacaagcgc
      181 ctttgccaga tcaaatctgg tcatggaatt tgactacacg tggaccagcg ccaacaccag
      241 ctacgacgcc atcatgatgg gttggaagga cgatggcact ggccggaccc agacaaattt
      301 ggtgtatgcc cattacaacg cgggtgcgag cacgtgcacc gtcaattgca ccggttacgt
      361 ctttgaagac gggacctccc ggagtggggt cacaagcaaa tacacacaaa atcaacctta
      421 ccgactccgc ctctccatga aaacagaagg cggtgccaca taccaacagt ccagcgatgc
      481 cggacaaacc tggtccactg tttacaattc agtccattcc acagaatcca acctgcaccc
      541 cggattcgtt ttgcattccg gcacgcatgt gatcgacaat gttcgcgtgc gtccctggac
      601 cagcacggaa ccgactgtga gcatcgatgc agaagtttct caatacagcg ctgtcagtgg
      661 caccctgctc tccagtgttt atgacacggg agaggccggt tgcgccgttg agagcatcgc
      721 acacacagtg agcgataccg gtttggttga aatcagactg cgctcagcca acgcacctaa
      781 catgtcggac gccaccgatt tttcggcctg cgatgccatt gacggaagca cctcattcac
      841 cgcctacccc tgcgtgcagg ctcacgaccg ctatctgcaa taccagttgg ttttgggaac
      901 ggaagaagac cttggcccca cactcaccga catcacgatc aattatgatt ctttgtccct
      961 tcaagccaac gccggtgaag accgtattgt agcggcaggc cgcgccatca cccttgatgg
     1021 tttggcctca acaggcattg atctctccta cgcctgggcc gtgtttgagg gcgagggagt
     1081 tcttgctgac gaattcacag ccacccccac gctcaccatt cccgcaggcg cacaacgatc
     1141 acaaaccagc gtgaccctga ctgtgtacga tgcctacggc caacacgcct ccgacgaagt
     1201 gaccttatcg attttgggca cacccactct ctcgctggcc gaaaacaaat tggc
//
LOCUS       DATDUN010000135         1064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_255856, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000135 DATDUN010000000
VERSION     DATDUN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255856"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="amrB"
                     /locus_tag="VJC18_01860"
     CDS             complement(<1..767)
                     /gene="amrB"
                     /locus_tag="VJC18_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018877605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HLD44154.1"
                     /translation="MLLKIRKPAVAGMFYPAEPQVLKQDIQTYLSQVQDCALVPKAII
                     VPHAGYIYSGQVAAQAYAQVSTIKDKIKRVVLLGPSHRVGFRGAALTNARCYNTSLGD
                     VFLDTTAHEVLAGLPKVVVFDQAHVPEHSLEVQLPFLQVVLHDFVLVPVVVGDARPEE
                     VGEMLCRLWGGEETLIVISTDLSHFEDYASACAHDARTCRLVEDMAYDSFEHDDACGR
                     VPLGGMLRAASQKGLRIKTLELKNSGDTAGTKDRVVG"
     gene            837..983
                     /locus_tag="VJC18_01865"
     CDS             837..983
                     /locus_tag="VJC18_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44155.1"
                     /translation="MRSSLATGHWPLALIANTIPVVNPYQTPPQGQFMKPWHDAVLEP
                     EKMD"
BASE COUNT          257 a          317 c          245 g          245 t
ORIGIN      
        1 taccccacca cccgatcctt tgtccctgcc gtgtctcccg aatttttcaa ctcaagcgtt
       61 ttgatgcgca aacccttctg actggccgcc cggagcatgc cgcccagagg caccctgcca
      121 cacgcgtcgt catgctcaaa cgaatcgtaa gccatatcct caaccagacg gcaggtgcgg
      181 gcgtcgtgag cacaagcaga agcataatcc tcaaaatgac tcaaatccgt gctgatgaca
      241 atgagtgtct cttcgcctcc ccacaaccga cacaacattt ctcccacctc ttctggccgg
      301 gcatcaccga ccaccacagg aaccaaaaca aaatcgtgca ataccacctg taaaaaaggc
      361 aattgcacct cgaggctgtg ctcggggaca tgggcttgat caaaaacgac cacctttggc
      421 agtcctgcca acacttcgtg agccgttgtg tccagaaaaa catcccccag gcttgtgttg
      481 taacaccggg cgttcgtcaa cgcggccccc ctgaacccca cccgatgcga aggcccaagc
      541 aaaaccaccc gtttgatttt atcttttatt gtgctgacct gcgcataggc ctgcgccgcc
      601 acctggcccg aataaatata gcccgcatgg gggacaatga tcgccttggg cacgagagca
      661 caatcctgca cctgtgacaa ataagtttga atgtcttgtt tgaggacttg gggctcagcc
      721 ggataaaaca taccagccac cgcaggtttt cggattttga gcaacatcta ttcgtgaatc
      781 gtgaatcgtg aatcgtgaat cgcctgtgcc ctttagggta tggagcgaaa cacgatatgc
      841 gttcttcact agccactggc cactggccac tggcactaat cgccaacact attcccgttg
      901 tcaaccccta tcaaacaccc ccacagggtc aattcatgaa gccttggcac gacgctgttt
      961 tggagccgga gaagatggat tgaccattca cgaggctgag tattgatatg agtcagctct
     1021 ggtttacgtg cgctgatttt tttttgttcg taggggtcgt tttg
//
LOCUS       DATDUN010000136         6416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_588117, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000136 DATDUN010000000
VERSION     DATDUN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588117"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..782
                     /locus_tag="VJC18_01870"
     CDS             117..782
                     /locus_tag="VJC18_01870"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLD44156.1"
                     /translation="MKNNQKAKTILTDFQTQVLRELFLDEWFRRYFYLTGGTALSEYY
                     LHHRYSDDLDFFSHDIDIKPFLGLIKKIGKKLNQKIQPITTAPSFMRFTIGDNLKIDV
                     VGDVGFRVGVPELIDGMMIDSIKNLAVNKVGAILGRLDAKDYVDLYFLLSEYSFDVFD
                     LLELGIKKDAGLEPFIWSTIIADVDRLMLLPRMIKPVTFEELKTFYFALRDKILDRIK
                     PSE"
     gene            944..2074
                     /locus_tag="VJC18_01875"
     CDS             944..2074
                     /locus_tag="VJC18_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HLD44157.1"
                     /translation="MSKLRLTFGKLVQSIIDTHQGCLGRATKAVNVNLTLRNWLIGYY
                     IQEYTKKGVDRATYGERLVEKLSVVLEKRNVPRCDRRELYRYCQFYITYPQIVESLPP
                     QFKLQIIAKTKNSKIVDSVTPQLCLGGKQLLETLSFTHFAELLNVDDSLKRVFYEHEC
                     LRGNWSVRELKRQIDSLYYERCGLSKNKQKLVTIVCTQSGRTQPEDVIRDPYVFEFLG
                     IKPQEVMDESTLETALLNKLQSFLLELGHGFCFEARQKRIPIDDEFFYVDLVFYHRVL
                     KCHILIEMKTTGFSHENLGQLNTYVNWYRKHEMSDGDNPPIGILLCTRKNQALVEYAK
                     AGITNKLFVSRYQLLLPKKEEIQKYLTDQMRDLQSRNSRQLT"
     gene            complement(2141..4480)
                     /locus_tag="VJC18_01880"
     CDS             complement(2141..4480)
                     /locus_tag="VJC18_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009565613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HLD44158.1"
                     /translation="MARAHPEAYELSDTEKRDLIKLINEDKPLPEKYRFLLFADKHEV
                     ELVWNGKSREVCTAILPFQTLEHIDEPRKEKQNDQMLDLDIGGRQIKGWTNKLIWGDN
                     KLILSSLKSGALRRQIEDAGGLKLIYIDPPFDVGADFSMDIEIGGETFHKEPNLLEQI
                     AYRDTWGRGADSFIAMIYERLILMRDLLAKDGSIYVHCDWRVNAHIRLVLGEVFSTGN
                     FSSEIIRQKTLSRKAQSDGFGNIHETIYFYSKSNEYTFNPQIDVRDEAARDARFPEIE
                     EGTGRHYVWDNFTQAGTGPPRRFGEVVLDPPTSKHWIWSQERIDEGWKTGIVQIRGRM
                     PRLKRYETEGVPAGDVWMGNDFVLHAQSGEDTGYATQKPEGLLERIIKASSNEGDLVA
                     DFFCGSGTTAAVAEKLGRKWIATDLGKFGVHTTRKRLIQVQRELKSGGKPFRAFEVLN
                     LGRYERQAYLNVGGRLTGKKKEQALARKEQEFRDLILKAYKATPTNASGFFQGTTGNR
                     LVVVGPINLPVGRLFIEEVITECRKRNASRVDVLAFEFEMGLFPAVLDEAKQQGIDLA
                     PKTIPPEVFDKRAVEKGQVRFHEVAYIEVTPRYDKKNARSVAVELTDFSVYYSQGLVD
                     AISADLKEGKSEVVCEQGKLIKVNKNKAGIITHDILTKKWTDWVDYWAVDFDYESRKE
                     LIKVARGTGLNTVQGNLPGHEPAQRELDMPVANFEERWTGAYIFENEWQSFRTRQNRE
                     LELTTVNHTYNNPGRYVIAVKVIDIFGNDTMTLVPVTIG"
     gene            complement(4482..6416)
                     /locus_tag="VJC18_01885"
                     /pseudo
     CDS             complement(4482..6416)
                     /locus_tag="VJC18_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340046.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
BASE COUNT         1602 a         1473 c         1527 g         1814 t
ORIGIN      
        1 tgatgaagtg tttcggtatc tgactctcaa agaaatccaa tgcgtgtatc caaaaatcag
       61 ggccgatcaa aaactcaaac gtcgctggaa atacgcgcta caaaggtggg ccaaacatga
      121 aaaacaatca gaaggctaaa accattctca ccgattttca gactcaggtc ttgcgtgaat
      181 tattcctgga cgaatggttt agacgatact tttacctcac cggcggcacc gctctttccg
      241 aatactatct gcaccatcgc tattcagatg atctggattt tttctcacat gatattgaca
      301 tcaaaccgtt tctggggctc atcaaaaaaa taggcaaaaa attgaaccag aaaatccaac
      361 cgatcacaac agcaccaagt tttatgagat tcacgatcgg agacaatctg aagattgatg
      421 tggtgggtga tgtggggttc cgcgtggggg tgcctgaact cattgatggc atgatgattg
      481 actccataaa aaacctggcc gtgaacaaag tgggagccat tctggggcgt ttggacgcaa
      541 aagattatgt cgatttatat tttcttcttt ccgaatactc gtttgatgtt tttgatctgt
      601 tggaattggg catcaaaaaa gatgccgggc ttgagccctt catttggtcc acgatcatcg
      661 ctgatgtgga tcggctgatg ttgttgccac gcatgatcaa acccgttacc ttcgaggaat
      721 tgaagacatt ttattttgcc ttgcgagaca agattttgga tcgcatcaaa cccagcgaat
      781 aaacagtctt cgttttcgag cacgttcccc cccatcgcta tgatgctctc agttgtgtca
      841 ctcaatgctg gtcatcgtta ggtaatccgt caaaacgctt caacagatga cgtttttcga
      901 ttgattgata tttttgtgta tgtcatgtcc gcccatgggt aaaatgtcaa aactcagatt
      961 gacttttggt aaactcgttc aatccatcat tgacacccat caggggtgtt taggacgtgc
     1021 cacaaaggcc gttaatgtta acctcactct cagaaattgg ctcatagggt actatattca
     1081 ggagtacaca aagaaagggg ttgaccgcgc aacatacgga gagcggttag tggaaaaatt
     1141 atcggttgtt cttgaaaaac gaaatgtgcc tcgttgcgat cgcagagagc tttatcgtta
     1201 ttgccagttt tacatcacat acccccaaat tgtggagtca ttgcctcctc aattcaaact
     1261 tcaaattata gccaaaacaa aaaatagcaa gattgtggat tcagtgactc cacaattatg
     1321 tctgggtggt aaacaattgc tggaaaccct ttcctttaca cactttgctg agttattgaa
     1381 tgttgatgat tctttaaaga gggtttttta tgaacatgag tgtcttcgcg gtaattggtc
     1441 tgttcgtgaa ctaaaacgac aaattgattc tctatattac gagcgctgtg gtctttcaaa
     1501 gaataaacaa aaactggtaa caattgtttg cacacaatct ggacgcacac aacctgaaga
     1561 tgtgattcga gatccctatg tgtttgaatt tttgggcatc aaaccacaag aggtcatgga
     1621 tgaatcgaca cttgaaacgg ctttgttgaa taaattgcag tcttttctcc tggaattggg
     1681 gcacggtttc tgttttgagg ccagacaaaa acgcattcca attgatgacg agttttttta
     1741 tgtggatttg gttttttatc acagggttct taaatgtcat attctgatcg aaatgaagac
     1801 cacaggattt tcacacgaga atttgggaca gctcaatacg tacgttaact ggtatcgcaa
     1861 acacgaaatg agcgatggcg acaatccccc cattggtatt ctgctttgta ccaggaaaaa
     1921 tcaggccttg gtagagtatg caaaagcagg gatcaccaat aaactttttg tgagccgtta
     1981 tcagttgtta ttgccaaaga aagaagaaat tcaaaaatat ttgacagatc agatgcgtga
     2041 tttgcagtct agaaactcga ggcagttgac ataagccttg gcacaacgtg gtgggaagaa
     2101 atgacggatt cgacggtatc tgttgtttcg gcatcgttgt ttacccgata gtcacaggaa
     2161 cgagcgtcat cgtgtcattt ccgaatatat cgataacctt tacggcgata acatagcgac
     2221 cgggattgtt gtaggtgtgg ttgactgtgg tcaattcaag ctcacggttt tggcgcgtgc
     2281 gaaaactttg ccattcattt tcgaaaatgt atgcacccgt ccagcgttcc tcgaaattgg
     2341 caacgggcat atcaagctcc cgttgtgctg gttcatgacc aggcagattg ccctgtacgg
     2401 tattcaagcc agttcctcgc gcaaccttga taagctcttt ccggctctcg taatcaaaat
     2461 caacagccca gtaatcaacc cagtcggtcc atttctttgt gagaatatcg tgggtgatga
     2521 tgcccgcctt atttttgttt accttaatga gtttgccttg ttcacaaacg acctcactct
     2581 taccctcttt cagatctgcc gaaatcgcat ccaccagtcc ttgtgaataa tatactgaga
     2641 aatcggtcaa ctcaacagcc acagaccgtg catttttttt atcgtagcgc ggtgtaactt
     2701 caatataggc cacttcgtgg aagcgcacct gacctttctc tacggcgcgt ttgtcaaaaa
     2761 cttccggtgg gatcgtcttg ggggcgaggt caatgccttg ttgcttcgcc tcgtccagta
     2821 cggcagggaa caggcccatc tcaaactcaa atgccaaaac atctacacgc gaagcattac
     2881 gcttacgaca ttccgttatc acttcttcga taaacaatcg tcctaccggc aagttgattg
     2941 gtccaacgac cacaaggcga tttcctgtag tgccctggaa gaaaccactg gcattggtag
     3001 gcgtggcttt gtaagccttc agaatcaaat cacgaaactc ctgttccttg cgagcaagtg
     3061 cctgttcttt ctttttgcct gtaagccgtc caccgacgtt aagatacgcc tgtcgctcat
     3121 agcggccaag attaagaacc tcgaaagcac ggaatggctt gccaccagat ttcaactcgc
     3181 gttgcacctg aatgagccgc ttgcgtgtcg tgtggacacc gaacttaccg aggtcggtgg
     3241 cgatccattt gcgaccaagt ttttctgcca cagcagcagt tgtgccactt ccacaaaaaa
     3301 aatccgccac caaatcgcct tcgttgctgg atgcttttat gatacgttcg agaagtcctt
     3361 ctggtttttg ggttgcatag cctgtgtctt cgccactttg tgcgtgaagc acaaagtcat
     3421 tacccatcca aacatcacca gcggggactc cttcagtttc atatcgcttg agacgaggca
     3481 ttcttccacg aatttgaacg atgcctgtct tccatccttc atcgatacgt tcttgagacc
     3541 aaatccagtg tttgctggta ggcgggtcca atacaacctc accaaaccga cgcggggggc
     3601 ccgtccctgc ttgtgtgaaa ttgtcccaga cataatgtcg tcccgtccct tcttcgatct
     3661 caggaaaccg cgcgtctctc gcagcttcat cacgaacatc tatctgcgga ttaaatgtgt
     3721 attcgttgga tttgctgtag aaataaatcg tttcgtggat gtttccaaaa ccatcagact
     3781 gggcttttcg tgacagtgtc ttttgtcgaa taatctcact tgagaaatta cccgtggaaa
     3841 aaacttctcc caaaactaat cggatgtgag cattcacacg ccaatcgcaa tgcacataaa
     3901 tacttccgtc tttcgccaac aaatctctca ttaaaatcaa tcgctcataa atcatggcaa
     3961 tgaaggaatc cgcgccgcgc ccccaggtat cacggtaggc aatttgttcg aggaggttcg
     4021 gctctttgtg gaaagtctcg ccgccaatct cgatgtccat actgaaatcc gcgcccacgt
     4081 cgaagggtgg gtcaatgtag atgagcttta ggccacccgc atcttcaatc tggcggcgaa
     4141 gtgcgccact tttgagagat gatagaatga gcttgttgtc accccagatg agtttgttgg
     4201 tccacccttt gatctgccgt ccgccaatat cgagatcaag catctgatcg ttctgctttt
     4261 cttttcttgg ttcatctatg tgttcgagcg tctgaaaggg gagaatggct gtgcagactt
     4321 cacgagactt gccattccaa acaagttcga cttcgtgttt atcggcaaag aggaggaaac
     4381 ggtatttttc tggaagcggt ttgtcctcat tgataagttt gatgaggtca cgcttttcgg
     4441 tgtcggatag ttcataggct tctggatgtg cacgagccat attatgattg gtactccgta
     4501 aaactggttg cgagcgcatc aaaagttttg ggtaggtgtt gttcaaaacc ctgctgatca
     4561 acgtaaatga aacgataggc aacgccatct tcatcaacac tggcggaagt ggcgtcggcg
     4621 catcattgtt tcagtcgcag cattttctgt gggagatcga gttcctcgcg gcctttggtt
     4681 tcaatgatcc aaactatgcc gtcggtggta cgaacaatga aatccggcgt gtagttggac
     4741 aagtcgccat cggccttcac gtagtccagc ttaaatccaa tcgcgaggta gttcttggta
     4801 aacgccgcca catcgcttgc tgcttcaagg aaagcggcaa aagaaagttc gaagccgcca
     4861 gagttggcct caccgacgat ttttgtgaaa atggattttt ttgctgcaag aaaaggccga
     4921 ttctctgtgc gaaatgggcg cgtattacta agacggattc gatcttcaat gcgagtgttg
     4981 cctgtgtcgg caatggtcaa cgcgttgatg gctgttttga acgaatcgta gaggattttg
     5041 cccgcgtccg gttcggaaag attgcgcagg acgactggat cttcgaggtc cactggcgac
     5101 ggagtgaaca ggtgctcgcg catgaaggtt tttaccttgc catagaggac gtcgtaacca
     5161 ccgacaagtc gaaggtcttt gagcaactgg cgggcaaaga acgcgacaac tgaccggtag
     5221 tcggccggtc cagaaccatc gagctgaatg gtgtgatcaa tttcggcatt gagcattgtt
     5281 ttgaacacaa tttcgcgcgt ctcttccgga gtgaagggtt tcaggggcag tcgtttgttg
     5341 ttcaaggtag ccggattaag cgtgtcgaga ttcttgaact ctcggtgaaa gcgacgggca
     5401 agttttggga gtggaatatc gagttcatct atttttttgt ctatgttctc ggtatccact
     5461 tcaacgatga gcgaatcttt ccgcgttgag ccgccaccca ttggaacatg ttcaaagctg
     5521 actccctcgg tctggatgga ttctacaaac tccataaatg caggcgtacc cataacagac
     5581 acagtttcac gttggtcggt accaaaatac attcggcgca ggccgcgacc tagcgtttgt
     5641 tcgggaagga tattgctttg cgcgacatag gcacggagcc cgacaatagt agtgacattg
     5701 cgtacgtccc agccttcctt gagcatgagt acggacacga tggccttgtg tggggaggcc
     5761 caagtatcga tgtggttggc ttctttgcgg agttcttcca attcttcctt gtttttaccg
     5821 gatgcggctt cggaaatttc tccattgttt ttggtgtgaa taacgagtac cgaatcttgc
     5881 agttcggggc aaattttttc gaggtacgcg cccacgtcgt cacagttgcg cgtgtcgtcc
     5941 accatgacga agaggacggc ttttttccca agcagttcgt gttcagcgta gcttttcttc
     6001 cattcctcta tgccgagctt gatgtagtca tcgtattttt cggtgaagat ggcacttctc
     6061 ttctcctcaa gctttgcacg gctcaccgca tcgggcagga cgggatgttt aacgacgttc
     6121 tgatgaatag cctcgaccag cggatagtcg gaaaccgttt ggacaaaaat agctccattg
     6181 tcgtggcgcg gtgtggcggt tacgtctatt tgcaaggcta ggcgacgatc cttttgcagc
     6241 atcttatggt gaatgtcttg aatggattta aaccacgcca tttttgggtt gtgaatgtgg
     6301 tgtgcttcat cattgaaaat tgccagttct tcgatttcgc ggacaatttc gcccaggtct
     6361 gttttgctgt cggtggtttt ccctactggt ttgggaccaa atggatcgag aaagta
//
LOCUS       DATDUN010000137         1075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_340417, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000137 DATDUN010000000
VERSION     DATDUN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340417"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VJC18_01890"
     CDS             complement(<1..255)
                     /locus_tag="VJC18_01890"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLD44159.1"
                     /translation="MSLSAKYWDEFYTKSVFQNGKEPTPFLREMLPRLQKGKVLDVGM
                     GEGVNAVFLAKQGFSVKGFDVSRVAVERAQALAAENSVTIE"
     gene            complement(443..>1075)
                     /locus_tag="VJC18_01895"
     CDS             complement(443..>1075)
                     /locus_tag="VJC18_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLD44160.1"
                     /translation="ETILLFVTCVWIIYEAVSRLLFEPVSVQVTVWSFLVVLLSIVVD
                     VSRSRALSRAAKKYRSQALEADALHFSTDIWSSAVVLLGLIFVKLHVFLPKYEFLHEA
                     DAVAALLVAAIVVYVSFRMGFKTVQALIDTAPSGLEKKIVVLVEAISGVKNCHQVRMR
                     YSGARLFIDAHVAMNGSLTLDEAHQLMDEIEAVISKEVPESDVTVHPEPL"
BASE COUNT          309 a          322 c          208 g          236 t
ORIGIN      
        1 ttcaatggtg acgctgttct cagcggccaa cgcctgtgcc ctctctacag ccacccggga
       61 cacatcaaag cctttgacac taaatccctg tttggccaga aacacggcgt tcactccctc
      121 gcccatcccc acatcgagca ccttgccctt ttgcagacgc ggcaacatct ccctcaagaa
      181 aggcgtgggt tccttgccat tttgaaaaac gcttttggta taaaactcgt cccaatattt
      241 tgcggatagt gacatgattt gcactcctta aaaccagctc acaatatatt ttttgtcatt
      301 ccagcgcacg ctggaatcca taattgtcga aaatatggat cccagccttc gctgggatga
      361 caagtcactg gatccctccc tgtgaagaca gtgaccacgc agggatgaca aattccagaa
      421 ggacgcctaa ccaacagcct tttcacaatg gctccggatg cacggtgaca tcagactcag
      481 gaacctcttt gctgatcacg gcctcaatct catccatgag ctgatgggcc tcatccagag
      541 tcaggctccc gttcatcgcc acatgggcat caataaacaa ccgcgcgcct gaatagcgca
      601 tcctcacctg atgacagttt ttcacgccag aaattgcctc aaccaacact acaatttttt
      661 tctccaatcc cgatggcgcc gtgtcgatga gcgcttgcac agttttaaaa cccattctaa
      721 aactcacata aaccacgatc gcggccacca acaaggccgc cacagcatca gcttcatgca
      781 ggaactcata ctttggtaaa aatacatgca atttgacaaa gatcaaaccc aacaaaacaa
      841 ccgcagagct ccagatatca gtactaaaat ggagcgcatc ggcctccaat gcctggctgc
      901 ggtatttttt ggccgcccga gacaaggcgc gagaacgcga aacatcaaca acgatggaca
      961 acaacacaac caaaaatgac caaacggtca cctgcacgct cactggttca aacaataatc
     1021 tgctgaccgc ttcgtaaatg atccacacac aggtcacaaa aagaagtatc gtttc
//
LOCUS       DATDUN010000138         1163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_380835, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000138 DATDUN010000000
VERSION     DATDUN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380835"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VJC18_01900"
     CDS             <1..331
                     /locus_tag="VJC18_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44161.1"
                     /translation="FLEIYKSYELQALQENMLIAIPALEALLQEGDLWDFANGQGTLG
                     GALLSFAREGFGGTYVARRVHKFQLHEAECIKRWSNSSHREQRLRKYLEGKGGLPRDI
                     AVFDFDI"
     gene            386..>1163
                     /locus_tag="VJC18_01905"
     CDS             386..>1163
                     /locus_tag="VJC18_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLD44162.1"
                     /translation="MLQADAQTISEIKRRKNFAIISHPDAGKTTLTEKLLLFGQAIQM
                     AGMVKAKRARQFATSDWMEIEKQRGISVASSVMQFDYADHQINLLDTPGHADFSEDTY
                     RVLTAVDSALMVIDSAKGIETQTKKLFQVCRDRHVPIMTFMNKWDRDSKNAFELIDEV
                     EQVLQLKCAIMTFPIGTAREFKGVYDCVNRCVRVFDPGEKTRDFDQKIFLSLEDPKLI
                     ECVGQELLDDLREALELIDGAGHAFDEKEYLKGVLSPVFFG"
BASE COUNT          288 a          272 c          321 g          282 t
ORIGIN      
        1 atttctggag atctacaaga gctatgaact gcaggccttg caggagaaca tgctcatcgc
       61 cattcccgct ttggaagcac tgcttcagga gggagatctg tgggacttcg ccaatggaca
      121 gggaaccttg ggtggggcct tgctatcttt tgcccgggag ggttttgggg ggacgtacgt
      181 ggccagacgg gtacacaaat ttcaattaca cgaagccgag tgcatcaaac gttggtcgaa
      241 cagttcacac agggagcaac gtctgcgcaa atatctggag gggaaagggg gcctgccacg
      301 cgatattgcc gtgtttgatt ttgatatatg attcgtcccc gacagggctg ttgcaaaatg
      361 ctgtttgatg tcataagagg cctgcatgtt gcaagcagat gcccagacaa tcagcgaaat
      421 caaacgccgc aaaaattttg cgatcatctc tcatcccgat gcgggcaaaa ccacgctcac
      481 ggagaagctg ttgcttttcg ggcaggccat ccagatggcg ggcatggtca aggccaaacg
      541 tgccaggcag tttgccacct cagactggat ggagatcgaa aaacaacgtg gcatttcagt
      601 ggcctcatcg gtgatgcagt tcgattatgc cgatcaccag atcaatctgc tcgacacacc
      661 cggtcacgct gatttcagcg aggataccta tcgagtgctc accgcggttg actctgctct
      721 gatggtcatc gattcggcca aggggatcga aacacaaacc aaaaaacttt ttcaggtttg
      781 ccgtgatcgc catgtgccca tcatgacctt catgaacaaa tgggaccgcg attccaaaaa
      841 cgcctttgag ctcatcgatg aggtggaaca ggttttgcaa ctcaaatgtg ccatcatgac
      901 gtttcccatt gggacggcgc gtgaatttaa gggtgtttat gactgcgtga accgctgtgt
      961 gcgcgtgttt gatcctggcg agaaaacccg ggattttgat caaaagatct ttcttagtct
     1021 cgaagaccct aagctgatag agtgtgtggg acaggaactg ttggatgatc ttcgcgaagc
     1081 gctggaactc attgatggtg cgggacatgc ttttgacgaa aaagaatacc tcaaaggtgt
     1141 gttgagtccc gtctttttcg gat
//
LOCUS       DATDUN010000139         1031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_172316, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000139 DATDUN010000000
VERSION     DATDUN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172316"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VJC18_01910"
     CDS             <1..874
                     /locus_tag="VJC18_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44163.1"
                     /translation="DTRRWIPGAYIQFLRIDGIKWSDPIIDESLIEGTLSAVVKQIDE
                     KLKAHNRVRVDITQLVEKRSYIFPIVALQQIVRNTIMHRTYEGTNAPVHVYWFNDRIE
                     IMSPGGTYGAVNAETFGKPGYVDYRNPHLADAMKVLGFVQRFGVGIQTAREALKQNEN
                     PPAIFELAPTLVKCIIRVPKTKPNTPVISVNYSGHVQKKAESGLESPTQSSTQSTDPV
                     TKLVMVLKASELSASELRDKLKIKHRHTFRTNYLRPALENGLIEQTLPDKPNSRLQKY
                     RLTEKGCKMVEGKK"
     gene            874..>1031
                     /locus_tag="VJC18_01915"
     CDS             874..>1031
                     /locus_tag="VJC18_01915"
                     /inference="COORDINATES: protein motif:HMM:NF045002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44164.1"
                     /translation="MRKLASIRKVDGIIPHDNADTLEIAAVGGWQVVVKKDSFKTGDL
                     ACFCEVDS"
BASE COUNT          310 a          224 c          256 g          241 t
ORIGIN      
        1 agatacccgc cgatggatcc ctggggccta cattcagttt ttgagaattg atggaatcaa
       61 atggtcggat cccatcattg acgaatcatt gatcgagggc accctgtctg ctgttgtcaa
      121 acagatcgat gaaaaactca aagcgcataa tcgagtgcgt gtggacatca cacagttggt
      181 agaaaaacgt tcgtatattt ttcccatcgt ggcgttgcag cagattgtca gaaacactat
      241 catgcacagg acctacgagg gaaccaatgc accggtgcat gtttattggt ttaatgatcg
      301 catcgaaatc atgagtcctg gtggcaccta tggcgcggtc aacgccgaaa cgtttggaaa
      361 acccgggtac gttgattatc gcaatccgca ccttgccgat gccatgaaag tgttggggtt
      421 tgttcagcgg tttggagtgg ggatccaaac agcacgggag gcattaaaac aaaacgaaaa
      481 tcccccagcc atttttgagt tggcaccaac tctggtaaag tgcatcatta gagtcccgaa
      541 aacaaaacca aatacaccag taatatcagt caattatagt ggccatgttc agaaaaaggc
      601 ggagtcaggg ctagagtcac cgacccagtc atcgacccag tcaaccgacc cagtcaccaa
      661 gttagtaatg gtcttaaagg ctagtgagct atcggccagc gaacttaggg ataaacttaa
      721 aattaagcat cggcatacgt ttagaacgaa ttatttgcgt ccagctcttg aaaatggact
      781 catcgagcag actttgcctg acaaacccaa cagtcgactg caaaaatatc ggctgacaga
      841 aaaagggtgc aagatggtgg aggggaaaaa gtgatgagaa aattagcttc aataagaaaa
      901 gttgatggga ttatccctca cgacaacgcc gacacacttg agatagctgc tgttggtggg
      961 tggcaagtcg tggtcaaaaa agatagcttc aaaaccggtg acctggcttg cttttgcgag
     1021 gtcgattctt t
//
LOCUS       DATDUN010000140         1604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_340565, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000140 DATDUN010000000
VERSION     DATDUN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340565"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VJC18_01920"
     CDS             complement(<1..454)
                     /locus_tag="VJC18_01920"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HLD44165.1"
                     /translation="MSLDTTFNKIVYVKLKTLGLILLSNLIVLFGVLFFSGRIGEVVF
                     FFWFDCALVYTMTLFKIRVSGASRHDPHLTAILRGFALKGFAGTFLVTSLFITLMQYW
                     DLFFYGTRIPVLLIFCTLSLFKEVNAYRADFIGKKQFQFFSIPHLKWDF"
     gene            complement(460..>1604)
                     /locus_tag="VJC18_01925"
     CDS             complement(460..>1604)
                     /locus_tag="VJC18_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLD44166.1"
                     /translation="EAERYFVPRPTSDLPGESNFYTYFPRGTCLTISPWNFPCAILCG
                     MTVAPLVCGNPVIMKPSNQAPGIGYRLFQILIEAGVPNEALHFLTGSGATVGDYLTKH
                     PAIHVIAFTGSREVGLNIIKEASVIHPGQRHIKKVIAEMGGKNAIIIDDDADLDLAVT
                     HCLHAAFGFSGQKCSALSRLIVLDNIYDKFKTRYLEGMKSLTVGAAHETNISLGPVID
                     KPSRDRLMAVIERNRKNIIGQLELTASLMEQGNFVPPTIFEATDFQSELGQVEFFGPL
                     VTLFRVKNIDEAIKSANDVDYALTGGLFSRSPANIERIKHEMECGNLYINRANTGALV
                     GRQPFGGFKLSGIHGSKAGGPDYLLHFLEPKTITENTMRRGFAPEA"
BASE COUNT          403 a          383 c          439 g          379 t
ORIGIN      
        1 caaaatccca cttgagatga gggatggaaa agaattgaaa ctgttttttt ccgatgaaat
       61 ccgcgcggta ggcgttcact tctttgaaca gggacagcgt gcagaaaatg agaagcacgg
      121 ggatcctcgt tccataaaag aagagatccc aatactgcat gagggtgatg aacagggacg
      181 ttacgagaaa cgttcccgca aaacctttca gggcaaaacc ccggagaatg gctgtcagat
      241 ggggatcgtg tcttgatgcg cccgagactc tgatcttgaa cagagtcatt gtgtaaacca
      301 gcgcacaatc aaaccagaag aaaaaaacca cttcacctat tcttccgctg aagaacagca
      361 cgccgaacaa cacaatcaag ttgctcagca agatgagtcc cagcgtcttg agtttgacgt
      421 acactatttt attgaacgtg gtatcaagac tcatgctctt taagcttcgg gcgcaaaacc
      481 tctgcgcatg gtgttctcgg tgatggtttt gggttccaaa aagtgcagga gataatccgg
      541 cccgcccgcc ttggaaccat ggatgccgga gagtttgaac ccgccaaagg gctgacgacc
      601 caccaaagcg cccgtgttgg cacggttgat gtagaggttg ccgcattcca tctcgtgttt
      661 gatgcgttca atgttggcgg gactgcgcga aaacaatccg cctgtgagcg cgtaatccac
      721 gtcgttggcg gatttgatgg cctcatcaat attctttacc ctaaacaacg tgaccagtgg
      781 gccaaagaat tccacttggc ccaattccga ttggaaatct gttgcttcga aaatggtggg
      841 aggcacaaag ttgccctgtt ccatgagtga ggcggtgagt tcaagctgtc caatgatgtt
      901 tttgcggttg cgttcaatca cggccatcag acgatcgcgg gaaggcttgt cgatcactgg
      961 tcccaagctg atgttggttt catgcgccgc gcctactgtg agtgatttca tgccctcaag
     1021 gtaacgggtc ttgaatttgt cgtagatgtt gtcgagcacg atgagtcttg agagggccga
     1081 gcatttttgt ccggaaaaac caaaggctgc gtgcaggcaa tgtgtgacgg ccagatcaag
     1141 atcggcatcg tcatcgatga tgatggcgtt cttgcctccc atctccgcaa tcactttttt
     1201 gatgtgtctt tgtccggggt gaatgacaga ggcctccttg atgatgttca accccacctc
     1261 gcgagaaccc gtaaaggcaa tgacgtgaat ggcagggtgt ttggtgaggt aatcgcccac
     1321 tgtggcgcca ctgcctgtca aaaagtgcag ggcctcattt ggcacgcccg cttcaatcag
     1381 gatttgaaac aatctgtacc caatgcccgg tgcctgattg gacggtttca tgatgacagg
     1441 gttgccacaa accagtgggg ccacagtcat gccacacaaa atggcgcagg ggaaattcca
     1501 gggggagatg gtcaggcaag tgcctcgcgg gaaatacgtg taaaaattgg attcgccggg
     1561 cagatccgat gtggggcgtg gcacaaaata acgctctgct tcat
//
LOCUS       DATDUN010000141         4512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_422113, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000141 DATDUN010000000
VERSION     DATDUN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422113"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="VJC18_01930"
     CDS             <1..1973
                     /locus_tag="VJC18_01930"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HLD44167.1"
                     /translation="QRGTPADPLKEVGVTDQHGTKVWFKPDTDIFEETEYNFDTLSQR
                     LRELAFLNKGVHIVIRDERTEKEHDFLYNGGLKSFVEYLNQRKTPVHDSVVYFEDTRG
                     DVVVEVALQWNDGYNEVLFSFANNINTKEGGTHLSGFRSAMTRCLNAYSDANNLLKGL
                     TEKPSGDDIREGLCAIISVKLPHPQFEGQTKGKLGNSDIEGVVRQVVNDKFAQFLEEN
                     PPEARRIVSKIIDAARAREAARKARNLVRRKSALDTSTLPGKLADCQERDPAKAEIYI
                     VEGDSAGGSAKQGRNRANQAILPLKGKILNVEKARFDKMLSSEEIRILITALGTGIGD
                     NEFSIDKLRYHTIVIMTDADVDGSHIRTLLLTLFYRHMTDIISQGHLFIAQPPLFRVK
                     KGKSERYLKDQEALDAHLIEMAVGDVVVSVGDKTLTQSNLTILIKDIIDYDRHLNHFR
                     GKVDTRVIDAALMRTTLSLDVLANETELQKEIVKLKECLRSQQVSFNENDIVVSEDAE
                     HSRKRLTIKTQLGTTAMVTVIDTDFMRSAEVSQLFKIVSRIKSNAAPPYALVQKEEKY
                     IFDNLVALKNHVIDSGKKGLYIQRYKGLGEMNPEQLWETTMDPARRTLLKVQIEDAVE
                     CDQMFTVLMGDQVEPRREFIEANALNTRNLDI"
     gene            2103..2933
                     /gene="nifU"
                     /locus_tag="VJC18_01935"
     CDS             2103..2933
                     /gene="nifU"
                     /locus_tag="VJC18_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HLD44168.1"
                     /translation="MWEYTDKVKDHFLNPRNVGVIKDADGVGEVGSLACGDALTLYFK
                     LDEKGRIAEARFQTFGCASAIASSSALTEMIKGKTLEEAQQITNDDVASFLGGLPKEK
                     MHCSVMGFDALKKAIANYRGEKLPVHEGTIVCECFDVSDLEIKKAVQDHHLATIEEVT
                     NFTKAGGGCGKCHEKIQEIINETLLLHKVGPHKTKKLSNIQKIKLIEQTLEDVIRPAL
                     KKDHGDIELVDVSGNIVTVKLLGTCALCSKSPVTLKNFVESKLKELVSHDILLEEVKE
                     "
     gene            2930..4087
                     /gene="nifS"
                     /locus_tag="VJC18_01940"
     CDS             2930..4087
                     /gene="nifS"
                     /locus_tag="VJC18_01940"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HLD44169.1"
                     /translation="MKLIYADNNATTQVDPVVLDEMLPCLKDQYGNPSSMYTLAETSA
                     SKISEARKRVARLIGATCEEIIFTSCGTESNNAAIRSAIYTQPQKKHIVTTRVEHPAI
                     THLCETLAKNGYRVTFVPVDKKGRLDLDYLKKNLSEQTALVSVMWANNETGVIFPIAE
                     IAALVRKKGILFHTDAVQAVGKIPLSVMGGGVDLLSFSGHKIHAPKGIGVLFVRKGTP
                     FSPFLIGGHQEKNRRAGTENVASIVALGKACELAGEHLAEMNTRVKRMRDRLETELLQ
                     RIPQSEVNGDRLTRIPNTTNIGFSSIEGEAILLMLNELGVCASSGSACTTGSLEPSHV
                     LRAMKVPMTSAHGSIRFSLSTANQEEDIDYIIEHLPRIVAKLRDWSPFWKK"
     gene            4104..4355
                     /locus_tag="VJC18_01945"
     CDS             4104..4355
                     /locus_tag="VJC18_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HLD44170.1"
                     /translation="MTQTNINKTDELKKIEAILDKKIRPMLQADGGNLEVLSLEGNTL
                     GVRYQGACCGCPHAATGTLMAIESVLKDEYNPDIKVVPQ"
     gene            complement(4370..>4512)
                     /locus_tag="VJC18_01950"
     CDS             complement(4370..>4512)
                     /locus_tag="VJC18_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HLD44171.1"
                     /translation="VYEPYLIQEGFLLRTPRGRMVTELAYKHLGIPVNTKNKNGKCGD
                     LF"
BASE COUNT         1256 a         1103 c         1170 g          983 t
ORIGIN      
        1 accaaagggg cactcccgca gatcccctca aagaggttgg ggtgaccgat cagcacggta
       61 ccaaggtgtg gttcaagcca gacaccgata tttttgaaga aacggaatat aacttcgata
      121 cactttcaca aaggcttcgc gagctggcct ttctgaacaa aggggtgcac attgtcatca
      181 gggatgaacg caccgagaag gaacacgatt ttttgtataa cggaggactc aaaagttttg
      241 ttgagtacct gaaccaacgc aaaaccccag tgcatgactc tgttgtgtat ttcgaagaca
      301 cgcgcggtga tgtggtggtc gaagtggccc tgcaatggaa cgacgggtac aacgaggttc
      361 tgttttcgtt tgccaacaac atcaatacca aggaaggcgg gacacacctg agcggttttc
      421 gttctgccat gacacgctgt ctcaacgcct attccgatgc caacaacctt ctcaagggcc
      481 tgaccgaaaa accctctggc gacgatatcc gcgagggttt gtgcgccatc atttcggtca
      541 aactgcccca cccgcaattt gagggacaaa ccaagggcaa gctgggcaac agcgacatcg
      601 agggcgtggt gcggcaggtt gtgaacgaca aatttgcgca atttctcgaa gaaaatcccc
      661 cggaggcgcg ccgcattgtc agcaaaatta ttgacgcggc ccgtgcgcgc gaagcggcac
      721 gcaaggcacg caatctggtg cgtcgcaaat ccgcgctcga tacaagcaca ctgccgggca
      781 agctcgccga ctgccaggaa cgcgatcccg caaaggccga aatctacatc gtcgagggag
      841 attcggcagg tggctccgcc aaacaaggac gcaaccgcgc caatcaggcc attttgccgc
      901 tcaagggcaa gattctcaat gtggaaaagg cccggttcga caaaatgttg tccagcgagg
      961 aaatccgcat tctcatcacc gcgctgggga cagggattgg tgataacgaa ttcagcatcg
     1021 acaagttgcg ctatcacacc attgtgatca tgacagacgc tgatgtggac ggctcacaca
     1081 ttcgtacctt gttgctgacg ttgttttata gacacatgac cgacatcatc tcccaaggac
     1141 atttgttcat tgcgcaaccg ccgctctttc gagtcaaaaa agggaaatcc gagcgatacc
     1201 tcaaagacca ggaggccctc gatgcccatc tcatcgagat ggccgttggt gatgttgtcg
     1261 tgagtgtcgg ggataaaacc ctgacgcaat cgaacctgac catattgata aaagacatca
     1321 tcgattatga cagacatctt aatcatttta gagggaaggt ggatacgcgg gtcattgacg
     1381 ccgccctgat gcgcacaact ttaagcctcg atgtgctggc aaacgagaca gaactgcaaa
     1441 aagaaatcgt aaagcttaaa gaatgtcttc gctcacagca ggtgtctttc aatgaaaatg
     1501 acattgtggt ttcggaagac gctgaacatt cgcgaaaaag gctcacgatc aaaacgcaac
     1561 tgggaacaac ggcaatggtc acagtgatcg acaccgattt tatgcggtct gcagaggtgt
     1621 cacaactttt caagatagtt tcccgaatca agtccaatgc cgcccccccc tacgcgctgg
     1681 tgcaaaagga agaaaaatat attttcgaca acctcgttgc cctgaaaaac catgtcatcg
     1741 acagcgggaa aaaagggttg tacatccagc gctataaagg tctgggcgaa atgaaccccg
     1801 aacaactttg ggagaccacc atggaccctg cgcgtcggac cctgctcaag gtgcaaatcg
     1861 aagatgctgt cgaatgtgat cagatgttta ccgtgctcat gggggatcag gtggagccgc
     1921 gtcgagaatt tattgaggcc aacgcgctca acacgaggaa tttggatata tgaaatcgtg
     1981 cgtacgtgtt ggcgtgcgta cgtgcgtgcg gttctgatcc gtattctcag ggacacatgc
     2041 gattcatacc ctaaagggca caagcgatcg tcgagccacg atcatcgatt cacgaggttt
     2101 atatgtggga atacacagat aaagttaaag accatttttt gaatccccgt aacgtgggcg
     2161 tcatcaagga cgccgatggt gtgggcgagg tggggtcgtt ggcctgcggc gatgccctga
     2221 cgttgtactt taaactcgac gaaaaagggc gtattgccga agcgcggttc caaacctttg
     2281 gttgtgcgag tgccatcgcc tcttcatccg ccctcacaga gatgatcaag ggtaaaacgc
     2341 tcgaggaggc gcaacaaatc acgaacgatg acgttgccag ttttttggga ggactgccca
     2401 aagaaaaaat gcactgttcc gtgatggggt ttgacgcgct caaaaaggcc attgcgaatt
     2461 atcgcggcga aaaattgccc gtacacgaag gcacgattgt ttgtgagtgt tttgatgttt
     2521 ccgatttgga aattaaaaag gcggtgcagg atcatcacct ggcgacaata gaagaggtga
     2581 cgaattttac caaggcaggc gggggctgtg gcaagtgtca cgagaaaatt caggagatca
     2641 tcaacgaaac cctgttgctt cataaggttg gcccccacaa aacaaaaaaa ctttcgaata
     2701 ttcaaaaaat caagctcatc gaacaaacgc tcgaagatgt gattcgcccc gcactcaaaa
     2761 aagatcatgg cgacatcgaa ttggttgacg tgtcgggcaa tatcgttact gtcaagttgc
     2821 tggggacttg cgccctgtgt agcaaatcac ccgtcacact caaaaatttt gtagaatcca
     2881 aactcaaaga gctggtcagt cacgatattt tactggagga agttaaggaa tgaagctcat
     2941 ttacgccgat aacaatgcca ccacccaagt tgatcccgtg gtattagacg aaatgttgcc
     3001 ctgcctgaag gatcaatacg gcaatccgtc gagcatgtac acattggcag agacatcagc
     3061 gagcaagatt agcgaagcca ggaagcgggt ggccaggctc attggggcaa cgtgcgaaga
     3121 aatcattttt acaagctgtg ggaccgaatc caataacgcg gccatcagat cggccattta
     3181 cacacagcca caaaaaaaac acatcgttac caccagggtc gaacatccgg ccatcaccca
     3241 tttgtgcgag accctggcca aaaacgggta ccgcgtgacc tttgtgccgg tcgataaaaa
     3301 aggacgcctc gatctggatt atctcaaaaa aaatctcagt gagcagacag ccttggtgag
     3361 tgtgatgtgg gccaacaatg aaacaggtgt gatctttccg atcgccgaaa tagcggcctt
     3421 ggtgcgaaag aagggcattt tatttcacac cgatgccgtg caggcggttg gcaaaatccc
     3481 tctcagtgtt atggggggcg gcgttgacct gctgtcattt tccggacaca aaatccacgc
     3541 gcccaaaggc atcggagttt tatttgtccg caaggggact cctttttcac cgtttctcat
     3601 tggtggtcat caggaaaaaa acagacgggc gggtacagag aacgtggcct ctatcgtcgc
     3661 tttaggcaaa gcatgcgaat tggccggaga acatcttgcc gaaatgaaca cgcgggtcaa
     3721 gcgcatgcgc gatcgattgg aaacagaatt actccaacgc attccacaaa gcgaagtgaa
     3781 cggtgatcgc ctcacgcgta ttcccaacac aacaaatatt ggttttagtt ccattgaagg
     3841 ggaggccatt ttgctcatgc tcaacgagtt gggagtttgt gcctcttccg gatcggcctg
     3901 caccacaggg tcactggaac cgtctcatgt gctcagggcc atgaaggttc ccatgaccag
     3961 cgcgcacggg tccatccggt tcagcctgag tactgccaac caagaagaag acatcgatta
     4021 tatcatcgaa cacttgccgc gtattgtggc caagctcagg gactggtcac ctttttggaa
     4081 aaaataattt tttaggagga caaatgacac aaacgaacat aaataaaacg gatgaactca
     4141 aaaaaatcga agcgatatta gacaaaaaaa ttcgtcccat gttgcaggca gatgggggca
     4201 atctcgaagt gctctctctg gaaggcaaca cgctgggtgt gcggtatcag ggtgcctgct
     4261 gtggttgtcc acacgccgca acggggaccc tcatggcaat cgagagcgtc ttgaaggacg
     4321 agtacaatcc cgatatcaaa gtcgttcctc aataaaaaaa caacccggtt cagaacaaat
     4381 caccgcattt gccgtttttg ttttttgtgt tcacagggat gccgagatgc ttgtaagcaa
     4441 gttccgtcac catgcgtcca cgcggggttc ggagcagaaa accttcttga atcagatagg
     4501 gttcgtaaac at
//
LOCUS       DATDUN010000142         1639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_545459, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000142 DATDUN010000000
VERSION     DATDUN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545459"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VJC18_01955"
     CDS             complement(<1..623)
                     /locus_tag="VJC18_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLD44172.1"
                     /translation="MTNEEIRQWDKEHCVYCWKKQRSTQPIIMERAEGIYQYDIDGKR
                     YIDFCSGLLSINIGHGNKHVLDAMKAQMDKLCYVGSMFGTDVRAKLCKMISEVTPGDL
                     NHVFFTNGGAEAVENALKAARWYTGRQKIYSAWRGYHGATAGAISITGDPRRWPAEPA
                     VPGAGKFFGPYCYRCPFGYKNENECKMQCLEVLKTQVMIEGPKTIAA"
     gene            complement(738..>1639)
                     /gene="hydA"
                     /locus_tag="VJC18_01960"
     CDS             complement(738..>1639)
                     /gene="hydA"
                     /locus_tag="VJC18_01960"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HLD44173.1"
                     /translation="DGQLLAVLKNVGAHGGLVSVHAENGDMIVSLINEYLSQGKTAPE
                     YHWRSHPSIAEEEAVSRAIALADQAGYPIFIVHLSGKDGLQKIREARAKGMKVFAETC
                     PQYLLLPADLYTKPDFEGAKYVMSPPLRPADHQPKMWEGLADGSIQTVATDHCPFNFK
                     GQKDMGKNDFSKIPNGIPAIGDRFNLLYTYGVMAGKISLNRFVDVVATTPAKIFGMYP
                     QKGSIVVGGDADLVIFDPKVHTTISAKTHHHNVDYSVFEGFKLEGQPTDVLLRGKFAM
                     RDGKYVGEQGKGRFISRRTSGRV"
BASE COUNT          398 a          446 c          359 g          436 t
ORIGIN      
        1 atggccgcaa tggttttggg cccttcgatc atcacctgtg ttttgagcac ttccaaacat
       61 tgcattttgc attcgttttc gttcttgtaa ccaaacggac aacgataaca atacgggcca
      121 aaaaatttgc cagcaccagg cactgctggt tctgctggcc aacgccgcgg atcgcccgtg
      181 atggatatcg ccccagctgt ggccccgtga taaccacgcc acgctgaata gattttttga
      241 cgccctgtgt accagcgcgc ggctttgagt gcgttttcca cagcctcggc accaccgttg
      301 gtaaaaaaca cgtgattgag atcgcccggc gttacttcag aaatcatttt gcagagtttg
      361 gccctcacgt cggtcccaaa catggaaccc acatagcaga gtttgtccat ctgcgccttc
      421 atcgcatcga gcacatgttt gttgccatgc ccgatgttga tggacagcag gccagagcag
      481 aaatcaatgt agcgtttgcc atcgatatca tactggtaaa tcccttctgc gcgttccatg
      541 ataattggtt gagtgctccg ctgttttttc cagcaataca cacagtgttc tttgtcccat
      601 tgtctgattt cttcatttgt catgatattc ctttcgttga tcgcttgtgc cccagagggt
      661 atggatcgtt gatcgtggat cgtaaatgca tttctcgatt cacgatacac gacccacgat
      721 ccacagcaaa ttaatcttca cactcttccc gatgtccttc ttgatataaa tctccccttc
      781 ccctgttctc ccacatattt gccgtcacgc atggcaaatt tgcctcggag taacacatcg
      841 gtcggctgac cttcgagttt aaatccttca aacacagaat aatctacatt gtgatgatga
      901 gtttttgcac tgatcgtcgt atgcactttg ggatcaaaga tgaccagatc agcatcaccg
      961 ccaacaacaa tactcccctt ttgcggatac atgccaaaaa ttttggcagg ggttgtggcc
     1021 accacatcga caaaccggtt caaactaatt ttgcctgcca tgacaccata ggtataaagc
     1081 aggttaaaac gatctccaat agccggaatg ccgttgggaa tctttgaaaa atcatttttg
     1141 cccatgtctt tttggccttt gaagttaaac gggcaatgat cggtggccac agtttgaata
     1201 gaaccatcgg ccaacccctc ccacatcttg ggttgatgat ccgccggacg caaggggggc
     1261 gacatcacat acttggcccc ctcaaaatca ggtttggtat ataaatccgc tggcaacaac
     1321 agatattgcg gacaggtctc ggcaaagacc ttcatgcctt ttgcacgagc ctcacgaatt
     1381 ttttgcaaac catccttgcc cgagaggtgc acaataaaaa tgggataccc tgcctgatca
     1441 gcaagcgcga tcgcgcgaga cacagcctct tcttcggcaa tcgaggggtg cgaacgccag
     1501 tgatactctg gtgctgtttt gccctgactc aaatactcat tgatgagaga cacaatcatg
     1561 tcgccgtttt cggcatggac cgaaaccagc ccgccatgcg cccccacgtt tttgagcacg
     1621 gccaacagtt gaccatcgt
//
LOCUS       DATDUN010000143         1213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_588429, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000143 DATDUN010000000
VERSION     DATDUN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588429"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..824)
                     /locus_tag="VJC18_01965"
     CDS             complement(87..824)
                     /locus_tag="VJC18_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44174.1"
                     /translation="MAGIQGCQVVGGLCIGVAEEFLDQVDKITAGVALGKTVPGVFGD
                     ADDKGIRVVSAVDWARTDKSLALFAKPGNHPLIFEHGGDGDGVFEVLEATGWLDHTLN
                     SLCLSATFLPFFQSVIRTVLTMRRLLRFMTKIRLLLVMKNGKFSKRFTFTLWALDFMA
                     NMLGSILFFQKFIQDGFEGIENNQLEHIGNTFFHRCTSFSMFQVLHRSSPLIYDRHVC
                     LPGMPVSFLAKGLYLRDRNAIPPKSHD"
     gene            complement(834..1100)
                     /locus_tag="VJC18_01970"
     CDS             complement(834..1100)
                     /locus_tag="VJC18_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44175.1"
                     /translation="MREPPFAIGCDIRLIQASCGVSQRLAGKIAQPHPDGLVKEARSM
                     VGSGFKAECGLWGDSFFMQEGAEGIQGELPGIRTKSNGGKDFGF"
BASE COUNT          353 a          353 c          235 g          272 t
ORIGIN      
        1 atccgccaaa aatttcttga tctatacctc taaaatcgtt gaacaaaaaa ttgaaatcca
       61 cacgatcgac acaaaattgg ttgtcgtcaa tcgtggctct tggggggaat tgcgtttcta
      121 tcgcgcagat ataacccctt cgccagaaaa ctgacaggca tgcccggcaa acagacatgc
      181 ctgtcataaa tcaaaggaga tgatctatgc aagacctgga acatgctgaa acttgtgcat
      241 cgatgaaaga acgtattgcc gatatgctcg agctggttat tctcgatacc ctcaaatccg
      301 tcctggatga acttttggaa aaacaaaatc gagcccaaca tatttgccat aaaatcaagt
      361 gcccagagcg tgaatgtgaa cctttttgag aacttcccat ttttcatgac caaaagcagt
      421 ctgattttgg tcatgaatct caatagacgt cgcattgtca gaacagtcct gataactgat
      481 tgaaaaaatg gtaaaaacgt agctgataaa caaagggagt ttaaagtatg atccagccat
      541 cccgtagctt caagcacctc aaacacaccg tccccatcac caccgtgttc gaagatcagg
      601 gggtgatttc ccggtttcgc aaacaaggcc agcgacttat cggtccttgc ccaatccacg
      661 gcggaaacaa cccgaatgcc tttgtcatca gcatccccaa aaacacctgg tactgtttta
      721 cccaatgcca cgccggcggt gatcttatcg acctggtcca aaaactcctc ggctactcct
      781 atacacaaac cgccgactac ctggcatcct tgaatcccgg ccattttcac ccctcagaaa
      841 ccaaaatcct tccctccgtt cgatttcgtc cgtatacccg gcaactcacc ctggatccct
      901 tcagcccctt cctgcataaa aaaggaatca ccccacaaac cgcactccgc tttgaaaccg
      961 gagcctacca tggaccgggc ttccttcacc aaaccatcgg ggtgcggctg tgcgatctta
     1021 ccggccaacc tctgggatac gccgcaagac gcttgaatca aacggatatc gcaacctatg
     1081 gcaaatggcg gttcccgcac aatttcccaa aaagccaaat cctctataac taccaccgca
     1141 tcgcagccgc aaaaccacgg atactggtca tcaccgaatg cccctgggca tacgcgtcaa
     1201 cctaagagga atg
//
LOCUS       DATDUN010000144         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_213780, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000144 DATDUN010000000
VERSION     DATDUN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213780"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2233
                     /locus_tag="VJC18_01975"
     CDS             <1..>2233
                     /locus_tag="VJC18_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HLD44176.1"
                     /translation="KSLDVKLSQEHRIQFSPTDVVNYFHDPLITWLERHHIEMRTRQV
                     TTAEPCPSEFLTLLRQKGRTHEETVLQKMQSEKQVVMVSRDQDFTLERQKTLIAMEMG
                     AEVIYQPALSRASDPFVGHVDFLFKVKNSAGVKTRINGRDCDWHYVPCEVKLSKRAKP
                     EHLLQLSLYAHMLEDMQDYLPPSLYVINGKGETEAYRTQDYFDLSMHYREAFLTQQEL
                     FDANVVPLPHRLPLGDWHSYVDEYLTKCDDLRSLPGMMETQAVHLNNAGIRTITELSA
                     SPLKTVPQMPEPVFAKYQLLARLIVQAKTSGTIQFQLTDDLSDQKRGLQTLPPSAGKK
                     NDLFLILQASPLVQGDLCFNMGVTSYGTDTKDGVAQRGQLYQSWANDETDEERNFSNL
                     MDNIRRRLSHDRHMHVYVYGSSTTERLRKLAQKHGKHEALLDSFFERGVFVDLEKIFK
                     DSFLTSSVNTELVALAPLLGANGLSSEYRVDDAVMLAGHYFETEDAEIKQNALNRLGI
                     KTKADTALLFRMYQFLSSAQTQQKAKYQPRTRPLGPRPPFAKTFDGAHTTVDLKDTTL
                     LIQRLWGRFGSSIAAHQRGERTPDAERGRVALLLTQLLQYHIREKRAWFGQIQGRIEK
                     TPFELVADTVALGDMQLLPSAPTEVKHGKVFRFTFPAQETKVKQGLSCLVSQNLRVGV
                     KIEHIDFKQGEVSLRISDNMLTRLGGTMPETLALLPYDYVSPQAMHDAIYRIAQRLDQ
                     KEPR"
BASE COUNT          577 a          579 c          548 g          529 t
ORIGIN      
        1 caaaatcact ggatgtaaaa ctgtcacaag aacaccgcat ccagttcagc cctacagatg
       61 tggtcaatta ttttcatgat cccctgatca cctggctcga aagacatcac attgaaatgc
      121 gcacgcgtca ggtcactacc gcagagcctt gtcccagtga gtttttgacg ttgcttcgtc
      181 agaaaggacg cacacacgag gaaacagttt tgcagaaaat gcaaagtgaa aaacaggtcg
      241 tgatggtgtc acgcgatcag gatttcacct tggagagaca aaaaacgctc atcgccatgg
      301 aaatgggggc ggaggtcata tatcaacccg ccctgtctcg tgcgagtgat ccttttgtgg
      361 gacacgtgga ttttttgttt aaagtcaaaa attccgcagg agtcaaaacc cgtatcaacg
      421 gacgcgactg tgattggcat tatgtgccct gtgaggtaaa actctcaaag cgagccaaac
      481 ccgaacacct cctgcaactt agtctgtacg cccatatgct ggaggacatg caggactacc
      541 tgcccccatc cctgtacgtc atcaatggca agggagaaac cgaagcctac cgcacacagg
      601 attattttga tctgagcatg cattatcgcg aggccttttt gacccagcag gaattgtttg
      661 atgcgaatgt ggtgcctttg ccacacagat tgcccttggg tgattggcac agttatgttg
      721 atgaatacct gaccaaatgt gatgatctcc gcagccttcc gggcatgatg gagacgcagg
      781 ctgtgcatct caacaacgcg gggattcgca ccatcactga actgtctgct agtcccctca
      841 agactgttcc tcaaatgcct gaacctgttt ttgctaaata tcaattgctg gcccggctca
      901 tcgtgcaggc caagacctcc ggtaccattc agtttcaact cacggatgat ctttctgatc
      961 aaaaaagagg cctgcaaacg ctcccaccgt ctgccggtaa aaaaaacgat ttgtttttga
     1021 tcctgcaagc ctccccgttg gttcagggtg acctctgttt caacatggga gttacgtcgt
     1081 atggcacaga caccaaagac ggcgttgcac agagagggca gttgtatcaa agctgggcca
     1141 atgatgaaac agatgaagaa cggaattttt ccaacctgat ggacaatatc cggcgccgct
     1201 tgtctcatga tcgtcacatg cacgtgtatg tgtatggttc ctccacaaca gaaagactca
     1261 gaaagctggc acaaaaacac ggcaaacacg aggctctgct ggacagtttc tttgagcgtg
     1321 gcgtgtttgt ggatttggaa aaaatcttta aggacagttt tttgaccagc agtgtcaaca
     1381 ccgagttggt cgctcttgcg cctctgttgg gtgccaacgg gctttcctct gaataccgtg
     1441 ttgatgatgc tgtcatgctg gctggtcatt attttgaaac cgaagatgct gaaatcaagc
     1501 aaaacgcact gaaccgtttg ggtattaaaa ccaaagctga cactgccttg ctttttcgaa
     1561 tgtatcagtt tttgagttct gcccagaccc aacagaaagc caaatatcag cctcgcaccc
     1621 ggccactggg cccacgacca cctttcgcca agacctttga tggcgctcac acgactgtgg
     1681 accttaaaga caccacgctg ttgatccaaa gattatgggg ccgattcgga tccagcatcg
     1741 cggcccacca gcgcggggag agaacccctg acgctgagcg gggaagagtg gctctgctcc
     1801 tcacgcaact tttgcagtac cacattcgtg aaaagagggc ctggtttgga caaattcagg
     1861 ggcgaataga aaagacaccg tttgaactgg tggccgacac ggtcgcgttg ggagatatgc
     1921 aattgttgcc gtcagccccc acagaggtca aacatggcaa ggtttttcgg ttcacattcc
     1981 ctgcgcagga aaccaaggtc aagcaaggcc tgtcttgtct tgtgtctcag aatcttcgtg
     2041 tgggggtcaa gattgaacac atcgatttca aacagggaga ggtttccctc cgcatctccg
     2101 acaacatgct cacccgtttg gggggcacca tgcccgaaac cctcgctttg ctcccgtacg
     2161 attatgtttc cccgcaagcc atgcacgatg ccatttaccg aatcgcgcag agactggatc
     2221 aaaaagaacc ccg
//
LOCUS       DATDUN010000145         1495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_91068, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000145 DATDUN010000000
VERSION     DATDUN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91068"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VJC18_01980"
     CDS             <1..209
                     /locus_tag="VJC18_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLD44177.1"
                     /translation="VGRWQLPLTAHAIVLGGHVRVGFEDNIYYRKGELATSNAQFVER
                     VVRLAKELNRPVATVQQARELLCR"
     gene            complement(415..972)
                     /locus_tag="VJC18_01985"
     CDS             complement(415..972)
                     /locus_tag="VJC18_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HLD44178.1"
                     /translation="MQIYLHDKPIKYDGTQLVSHFAYNHFNLLGDAIVSFMGPCDVKI
                     DQLVDLEDVHQNAFIFSENMLHFIVEHFHDDLLLTIAWQRLLIDLIISEMKVDLPGGP
                     WERRGDDIYDDVFKVSVSIATRSPVSCLIHTGINISSNNTPVLTKGLDDYHINPQALA
                     TGVMNRYRAEIESMRKARAKVRGVG"
     gene            complement(1097..>1495)
                     /locus_tag="VJC18_01990"
     CDS             complement(1097..>1495)
                     /locus_tag="VJC18_01990"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HLD44179.1"
                     /translation="DDKTKTTQSAKAVWVPNRNGIFLNIAAGLAEMIEVDFLYTGFNV
                     EEAGTFPDNTQDFVSALNHCFTFSTLHNVMVQSPPIKMTKTQIVAELVKQNFPFELLW
                     SCYRGGEQMCGLCESCLRLKRALNACGLQA"
BASE COUNT          370 a          368 c          385 g          372 t
ORIGIN      
        1 ctgtgggtcg ctggcaattg ccgctcacgg ctcatgccat cgttcttggt gggcacgtgc
       61 gtgtgggatt cgaagacaac atctactatc gcaaagggga attggccact tcgaacgcgc
      121 agtttgtgga gcgtgtggtc cgcttggcca aggagctcaa tcgtcccgtg gctaccgtac
      181 aacaagcgcg ggaattatta tgtcgctgaa cgtggtatta tccaacccca acaccgacct
      241 tggcgcctgt tgtttatttc atgattgtgg cgcagacgct gtgagagcac agcgcataac
      301 acaagtattc gaatcatagg gcccgaatac ctgaagggcc aaattctgca aaactcgatt
      361 gagatcatgc gtcctgccgc gatcatgaaa taaacaacag gcgcaaaggt caactcaccc
      421 cactcccctc accttggcgc gtgctttgcg catgctctca atttcggctc ggtaccgatt
      481 catgactcct gtggccaggg cctgcgggtt gatgtggtaa tcatcgagcc ccttggtgag
      541 gacgggcgtg ttatttgaag aaatgttgat gccggtgtga atcagacagg acacaggtga
      601 gcgggtggcg atggacacgc tgaccttgaa cacatcatca taaatgtcgt cgcccctgcg
      661 ttcccaaggg ccgcccggca gatccacttt catttcgctg atgatgagat caatgagtag
      721 tctctgccac gcaatggtga gcaataagtc gtcatgaaaa tgctctacaa tgaagtgaag
      781 catgttttca gaaaagatga acgcgttctg atgcacatcc tccaagtcga ccagctgatc
      841 tatcttcaca tcgcagggcc ccataaagga gacaatcgca tcgcccagca gattgaaatg
      901 gttgtaagca aaatgcgaca caagctgggt gccgtcgtat ttgatgggtt tgtcgtgaag
      961 atagatttgc ataggtgaat caggcataag gaaaaacaac tgcttcaatt gtttcaacga
     1021 tcaactatat caattgccaa ttgtctgttg ctcattgaag tcgttgtagc aattgatccg
     1081 ccctaagcct ttagccttac gcctgaaggc cgcaggcatt caatgcccgt ttgagtcgca
     1141 ggcaggattc gcaaaggccg cacatttgct cgccgccacg gtaacaactc cacaacaact
     1201 caaacggaaa attttgtttg accaactctg ccacgatttg tgttttggtc attttaatgg
     1261 gtggtgactg gaccatcacg ttgtggagcg tggagaaggt gaaacagtga ttcagggcgc
     1321 tcacaaaatc ctgtgtgttg tcgggaaacg tgccagcctc ttcgacgtta aaacctgtgt
     1381 acaaaaaatc gacctcaatc atctcggcga gtcctgcggc gatgtttaaa aaaatcccgt
     1441 tgcgattggg gacccaaaca gccttggcac tctgtgtggt ttttgttttg tcgtc
//
LOCUS       DATDUN010000146         1145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_340752, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000146 DATDUN010000000
VERSION     DATDUN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340752"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..269
                     /locus_tag="VJC18_01995"
     CDS             132..269
                     /locus_tag="VJC18_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44180.1"
                     /translation="MTLLKRIFFFFSFIFLTALSAFASEADLAIPDLHKGAPYTLLGG
                     T"
     gene            496..>1145
                     /locus_tag="VJC18_02000"
     CDS             496..>1145
                     /locus_tag="VJC18_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HLD44181.1"
                     /translation="MKRFFVCGDPRFGLARFECSRCHHSLYVPFSCKTRLFCPSCHQK
                     KLELWLDDVRLHVVHQEMPHRFWTFSIPKRLRVYFQYRHKLLTLLVQAAKQTVFLTLG
                     NGKHWPFVAPGMITLIQTAGDELNFNCHLHALITDGVVNYADSHKITLQRCLYYDADQ
                     ITELFRLCVLRLLFKHHVIAQDVVDNMVTWKNSGFHVHVSESFTDPERMRICLAYS"
BASE COUNT          254 a          299 c          250 g          342 t
ORIGIN      
        1 aagcccttca tccagatcgg ccgctgtaat ggtgccgtcc ttgattttgt cagaagtgat
       61 ggcgtcatca gcaacactta acgcccgctg tgcaataata tgtccgcagg cgaaccctcc
      121 gtgacggaat catgacactc ctaaaaagaa tattcttctt ttttagtttt atttttctaa
      181 cggccctatc ggctttcgcc agcgaagcag atctggccat ccccgatctg cacaaggggg
      241 ccccttacac cctgcttggt ggcacatgaa ttgttgcttt gtaacttgcg ttgtcatgaa
      301 cctcagtttt gatcatcccg tatggcatca ttcttgcttt gactgtctgg catggcaagg
      361 agcctgcgat ttcgcggttt ttatgacatt ccattattcc aggtttttta taatcatttc
      421 gggtcgtttc aagaagaata cgcccgttgt tacgagtccc aactgggatt ctaccgtccc
      481 gtctgggacg agacaatgaa gcgattcttt gtgtgtggcg atccccggtt tggcctggcc
      541 cgttttgaat gtagccgctg tcatcacagt ctttatgttc ccttttcctg caaaacacgc
      601 ctgttctgcc caagttgcca ccaaaagaaa ctggaacttt ggctcgatga tgtcaggcta
      661 cacgtcgttc atcaggagat gccacaccgt ttttggacgt tttcgattcc caaaagacta
      721 cgcgtctatt ttcagtaccg ccacaaactc ttgaccctcc ttgttcaagc agccaaacag
      781 accgtgtttt tgaccctggg taatggcaag cactggccct ttgttgcccc cggcatgatc
      841 acactcattc aaaccgctgg cgatgagttg aatttcaact gtcatctcca tgccctgatt
      901 acagacggtg tggtcaacta tgccgattct cacaaaatca cgttgcaacg ttgtctctat
      961 tacgatgcag atcaaatcac agagcttttt cgcctatgcg tattgaggct tttattcaaa
     1021 caccacgtga ttgctcagga cgttgttgac aatatggtga cctggaagaa ttcagggttt
     1081 catgttcatg tcagcgagtc atttaccgac cccgaacgca tgcgtatatg tctggcgtat
     1141 tcctt
//
LOCUS       DATDUN010000147         1150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_588640, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000147 DATDUN010000000
VERSION     DATDUN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588640"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..>1150
                     /locus_tag="VJC18_02005"
     CDS             437..>1150
                     /locus_tag="VJC18_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44182.1"
                     /translation="MKLKTIKIQGFKSFADVTMVDFDPHITCVVGPNGCGKSNVVDAI
                     RWVMGEQSAKHLRGKNMEDVIFVGSESRPASALASVEITFAKDGTSAFPVQYENCEEI
                     SVGRHLFRSGESEYFINKQLVRLKDVTDFFLGTGVGTKAYAIIEQGRVEQLLTAKPEE
                     RRYIIEEAAGISKFKSRKEAALRRIEATNQNLSRLQDIILELERQVGSLDRQAKKAKR
                     FRVISDELKVLDLQLASLAY"
BASE COUNT          301 a          273 c          292 g          284 t
ORIGIN      
        1 taatcttaat gcttaatgaa cattaagatt atggaccaaa aaaaagagga agtcaatcac
       61 tcaaaatctt tcaaattggt catgttttct accacccccg ccctacgggc acccccgcct
      121 tacaacaggc gggggggcaa aatcaaaatt acctactttt atgtcgtaca cccaagggta
      181 tctgttcaag tcccgttaca ccaaggacag gcaaggtttc gtacgtgttt tagagtcaca
      241 gctctccact tccccctgac acttccccaa catcggatcg tcggcgcatc ggcgcgtcgg
      301 agcaaacaac cgacacccct ttattttgtt gattttgttg ttcttatcac attagtcttt
      361 acaagatcga cgcgtcaaac taatggggtc agagcttgtt gcaggcaggg gaatgcacat
      421 caaggcaggt gtccacgtga aattaaaaac tatcaagatt caagggttta agtcatttgc
      481 agatgtcaca atggtcgatt ttgatccgca catcacttgc gttgttgggc ctaatggttg
      541 cggcaagtcg aatgtggtcg acgcgattcg ttgggtgatg ggggagcagt ccgcaaaaca
      601 cttgcgcggc aaaaacatgg aagatgtgat ttttgtgggt tccgaaagca gaccggccag
      661 tgccctggcc agcgtcgaga tcaccttcgc caaagacggc acatccgcgt ttccggttca
      721 gtatgagaat tgcgaagaga tttcggttgg gcgtcacttg tttcggtccg gtgagagcga
      781 gtatttcatc aacaaacaat tggttcgctt aaaggacgtc accgattttt ttctgggcac
      841 aggagtgggc accaaggctt atgcgattat cgaacagggg agggtggaac aactgctcac
      901 tgccaagccc gaagagcgcc gctacatcat tgaagaagcg gccggcattt caaaattcaa
      961 atctcgcaaa gaagccgcct tgaggcgtat cgaggcgacc aatcaaaatt tgagtcgtct
     1021 gcaggatatc attttggaac ttgagcggca ggtggggtct ctcgaccgac aggccaaaaa
     1081 ggcaaagcgt tttcgcgtga tcagcgatga actcaaggtg cttgatctac aattggcctc
     1141 tttggcgtac
//
LOCUS       DATDUN010000148         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_340882, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000148 DATDUN010000000
VERSION     DATDUN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340882"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VJC18_02010"
     CDS             <1..341
                     /locus_tag="VJC18_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HLD44183.1"
                     /translation="FDKLAAGKTGTTSDYRDSWFVGYTPALLTLTWVGYKGNETTGLS
                     GASGALPIWTNFMKRATANRFYTDFEPTSKIIILPIDRKSRLLHQSSCGNDKYDEYFI
                     EGTEPSEFCK"
     gene            501..935
                     /locus_tag="VJC18_02015"
     CDS             501..935
                     /locus_tag="VJC18_02015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD44184.1"
                     /translation="MLIKRIILLFLVLTLVCTGCSVTKRKIAITKRPPLYEQTVLDTT
                     AHPVSGLEPAKELLKQGIGFLKNQDTDKAEWNFEEAIKIDPKYGPAYYWLARARYRLN
                     QTQSSFELLNKAEKLLQSSPDWLERVDQFRRFLLDKTAAPLQ"
     gene            1008..>2163
                     /gene="hisD"
                     /locus_tag="VJC18_02020"
     CDS             1008..>2163
                     /gene="hisD"
                     /locus_tag="VJC18_02020"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HLD44185.1"
                     /translation="MKILRTTDRNFRYDFGKVFKRQFEVPEAIFKSVADILDDIKRIG
                     DQALHKHTLKLDFFDLTGKSMEVTAKEMSSAIKEVSSEDKAALEMAASRIEEFYKSQK
                     LEKLAYKKTDVDIKILNKPLERVGIYVPGGKASYPSTVLMAAIPAKIAGVPEIVMCSP
                     WPSGEHNPAVLYAAQLAGVNRSFKLGGPQAIAAMAYGTKTLPKVDKIIGPGNIYVSLA
                     KKLVYGDVDIDMIAGPTELVILGDGSVPPAYVASDLLSQAEHDEMASTLLITTNANYA
                     IRVEGEVRKQVLKLKRKAIANESIKKRGLIVLVRNLEEGIDLVNEMAPEHLEICVKDA
                     EKIVEKISHAGAIYVGPYTPECVGDYIAGPSHILPTHGTARYFSAIRLSDF"
BASE COUNT          579 a          588 c          500 g          496 t
ORIGIN      
        1 ggtttgacaa actggccgcg ggcaaaaccg gcaccacgtc tgactaccgt gattcgtggt
       61 ttgtgggcta cacgccagca ttgctcaccc tcacgtgggt gggctacaag ggtaacgaaa
      121 caaccggcct ctcgggggcc agcggggccc tgcccatttg gacaaatttc atgaaaaggg
      181 ccaccgccaa caggttttac accgattttg aacccacatc caaaatcatc attctgccca
      241 tcgatagaaa atcaaggctc ctccaccagt cctcttgcgg caacgataag tatgatgagt
      301 attttattga agggacggaa ccgtcggagt tttgtaaata atttttttgt caacagtcga
      361 cggtttatgg ccaaacaata tctggccgtt agctgttggc cgtcggctgt cggcgagcat
      421 ttataaactt tcaatacatg ttctgaacat aatcgaagaa ttccttgttc actattcgat
      481 attccactat gcacaccgca atgttaatca aacgaatcat tttattattt ctcgtgctca
      541 cgcttgtgtg taccggctgt tcagtaacca aacgtaaaat cgccatcacc aagcgccccc
      601 ccctgtatga gcagaccgta ctggacacaa cggcacaccc tgtgtctggc ctcgagccag
      661 ccaaggaact gctcaaacag ggcatcggct ttctcaaaaa tcaggacact gacaaggccg
      721 aatggaattt tgaagaggcc attaaaatcg accccaaata tggtccagcc tattactggc
      781 tggcccgggc ccgttaccgc ctgaaccaga cacagtcctc atttgagctc ctcaacaagg
      841 ccgaaaaact cctgcaatcc tcgcccgatt ggttggaacg cgttgatcaa ttcaggcgtt
      901 tcctgttgga taaaacagcg gctccactac aatagccatc agctgtgacc caaatagcca
      961 agaaagactt agacatttcc ctataaattg actaaaagca ttgccctatg aaaattttga
     1021 gaacaaccga tcgcaatttc aggtacgatt ttggcaaggt ctttaaacgt cagtttgagg
     1081 tccccgaggc catttttaaa tctgtcgccg atattttgga cgacatcaaa cgcatcggtg
     1141 accaagccct tcacaaacac accctcaaac ttgatttttt tgatctcacc ggcaagagca
     1201 tggaagtcac cgccaaagaa atgagctccg ccatcaaaga agtttccagc gaagacaagg
     1261 ccgctttgga aatggcggca tcacgcatcg aagaatttta caaatcacag aaactcgaaa
     1321 aactggctta taaaaaaacc gatgttgaca ttaaaattct caacaaaccg cttgaacgcg
     1381 taggcattta tgttcccgga ggcaaggcaa gttatccgtc tacagtcctc atggccgcga
     1441 tcccagccaa aatcgcggga gtccccgaga tcgtcatgtg ctcaccctgg cccagtggag
     1501 aacacaaccc cgcagttttg tacgcggctc aactggctgg cgtgaatcgc tctttcaagc
     1561 tcggtgggcc acaggccatc gccgccatgg cttacgggac caaaacgctt cccaaggtcg
     1621 acaagatcat cggaccagga aacatctatg tctctttggc caagaagctt gtgtatggtg
     1681 atgtcgacat cgacatgatc gcaggtccaa ccgaactggt gatattgggt gacgggtccg
     1741 tcccgcctgc ctatgttgcc agcgatctgt tgtcgcaagc cgagcacgac gaaatggcca
     1801 gcactctgct catcacgacc aatgccaatt acgccattcg cgtcgagggc gaggtccgca
     1861 aacaggtgct caagctcaag cgcaaggcga ttgccaacga gtctatcaaa aaacgggggt
     1921 tgatcgtttt ggtccgcaat ctcgaagagg ggattgacct cgtcaacgaa atggccccag
     1981 agcatctgga gatttgcgtc aaagacgccg aaaagatcgt cgaaaaaatc tctcacgcag
     2041 gagccattta cgtgggaccc tacacgcctg aatgcgttgg cgattacatc gctggtccgt
     2101 cccatatttt gcccacgcat ggcacggccc gttatttttc ggccatcagg ctcagcgatt
     2161 ttg
//
LOCUS       DATDUN010000149         1766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_463213, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000149 DATDUN010000000
VERSION     DATDUN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463213"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VJC18_02025"
     CDS             complement(<1..562)
                     /locus_tag="VJC18_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44186.1"
                     /translation="MSIGNSNIFPKQPYGTPEIPSKPRIDPKTGLPIPEAERPDLTVP
                     EEPKPEAPKEEKETKTDSKQISEEAQQQVEERGGDLKTRTQQQQRSQQTRSSFEEDSG
                     ERAGSQLPDKWGHKININPEVARLRENPEGGEHPVHDATGQTQKKPEKEGLPQKEYTP
                     KMATDKDYRSVANEKFFRQGPLKDTPM"
     gene            667..>1766
                     /locus_tag="VJC18_02030"
     CDS             667..>1766
                     /locus_tag="VJC18_02030"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44187.1"
                     /translation="MITLSPHQDQAFLLKDYYGGAFAQQLVKTQRLARALVCVIVEEG
                     QAAEKLQDEILFFGPNSKIETLPYYDVSPYSGLSPLRRTCLKNQCVLFCLLSGQLDFL
                     IVPKHAALRRCLNKDIFSSLNITITKNDLIDRDELIQKLVLMGYERYSVVEEPGHFAV
                     RGDILDVFSPQHEHPHRISFFDIEIETIKPFTAVNQRTLEAVSEIQLIPAHEIFLHHY
                     HTTQQLREVPQTATTTPAWINTHWKSDLKKRADQKNIQKSKRDQIEEYVAHRIYFHGI
                     EFFLPLFYHETGTVFDYFKPNAIIVTDLVEPFDKFTGCFLEELSKTQQASDHIESIFA
                     PQDIFIDPKTLVEKLGPHPRLYRDTTGLEDLPA"
BASE COUNT          436 a          462 c          385 g          483 t
ORIGIN      
        1 gcatgggcgt atccttgagc ggcccctgac gaaaaaactt ttcattggca acagaccggt
       61 aatccttatc tgtcgccatt ttgggagtgt attctttttg cggaagccct tctttctcgg
      121 gttttttctg tgtctgaccc gtcgcgtcgt ggacagggtg ctcacccccc tcaggattct
      181 cccgcaggcg agccacttcc ggattgatat tgattttatg tccccattta tcgggaagtt
      241 gcgaaccggc cctctcccct gaatcctctt caaacgagga gcgcgtctgt tgcgaccttt
      301 gctgttgctg tgttcgtgtc tttaaatcgc caccccgctc ttcgacctgt tgctgtgcct
      361 cctcggagat ttgtttgcta tccgtcttgg tctctttttc ttctttgggc gcttcgggct
      421 tgggttcttc gggcacagtc aaatctggtc tttcagcttc cgggataggg agtcccgtct
      481 ttggatcaat cctcggtttg gacggtatct ctggagttcc ataaggttgc ttggggaaga
      541 tatttgagtt accaatggac ataataaaag tttattcttt aattctaata ggttatcggt
      601 tatttggcaa cgaaagtttc cattgattgt attttagacc tgtaaccaaa gacaaaagtc
      661 gttgaaatga taaccctatc acctcatcaa gaccaagcct tcctcctcaa agattattat
      721 gggggggctt ttgcacagca gcttgtaaaa acacaacggc ttgccagggc cctggtgtgc
      781 gtcattgtcg aagaggggca ggccgcagaa aaactgcaag atgagatttt gttttttggc
      841 ccaaactcca aaatcgaaac actgccgtac tatgatgtgt ctccttattc aggactatcc
      901 ccgctcagaa ggacgtgtct caaaaaccaa tgcgtgttat tttgtttgtt atcgggacaa
      961 ctcgattttt tgattgtgcc caaacacgcg gccctgaggc gctgtctcaa caaagacatt
     1021 ttctcatcac tcaacatcac gataaccaaa aatgacttga tcgacaggga tgagctcatt
     1081 caaaagctcg tcctcatggg gtacgaacgt tattctgtcg ttgaagagcc cgggcatttt
     1141 gccgtgaggg gagacatcct cgacgtgttc agcccacaac atgaacaccc tcaccgcatc
     1201 agttttttcg acattgagat tgaaaccatc aaacccttta ccgcggtcaa tcaacgcacc
     1261 ctggaggcag tgagcgaaat ccaattgatc cctgcccacg aaattttttt gcaccactac
     1321 cacaccaccc agcagctccg tgaggtaccg caaacagcaa ccacaacacc tgcctggatc
     1381 aacacccatt ggaaaagcga tctcaagaag agggccgatc agaaaaatat ccaaaaatcc
     1441 aaacgcgatc aaattgaaga atacgttgcc catcgcattt actttcatgg catcgaattt
     1501 ttcttgccct tgttttatca cgaaacagga acggtgtttg actacttcaa acccaacgcg
     1561 atcatcgtga ccgacctggt ggaacctttc gataaattca caggctgttt tctggaggaa
     1621 ctatccaaaa cgcaacaagc ctctgatcac atcgaaagca ttttcgcgcc acaagatatt
     1681 ttcatcgatc caaaaacact tgtggaaaag ctcggcccgc acccacgtct gtaccgtgac
     1741 acaactggct tggaggacct gcccgc
//
LOCUS       DATDUN010000150         1775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_214135, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000150 DATDUN010000000
VERSION     DATDUN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214135"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1775
                     /locus_tag="VJC18_02035"
     CDS             <1..>1775
                     /locus_tag="VJC18_02035"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HLD44188.1"
                     /translation="YIVLNLGIFLMAWFRAWRILNYLAFKFTFGVAVVWLLTRYEPVF
                     LLSSEMFFTAFFVLFFIISIVYAVKQPVKLKGLVDGSLVFGLPIVYFYMQSVLLKPYE
                     YGLAYLAAIMVVVYFGTAFVLRKMFQDRMEALIQSYYAIGVVFITLVGPLALSAQWTS
                     AYWALQGAGLVWIGVRQARTLARFSGLALIVVAGFSFTSLFEGKPVSLDYVIPVLNAD
                     FLSSVIKALTALYAGLYLRRQTNDKLFHAEAEKGIAAFVVLYGLCWWYLGLGREIMLH
                     APLGSQNLLALVLVIGSYLVLKPLARVTQDSFLKQFNLITAAVAAPIILERYWISLAW
                     SCLSVDLVCQLKVMYGDAAKKDSDLVFRHVILLLLMVLFNGILFFHWQSHPALISLGN
                     LSVCVFAVSAFFVAWVLYSETSKMRGDLISHVMILFVFGVFWFVGINYIDQAVSYVFA
                     GKDQVIMIFMLSTLVLSQALHARLYWKAATFPIQVAILPLTAVVFVWDWTNAHPAGDL
                     GWVTHILLIGLMAWSCWLWQHHAKKPLEEDLITAPFTKVVHWFGVLFMISAVTLEAQW
                     LAKGLVPAHPVWYDLVFCLIPAGFL"
BASE COUNT          305 a          390 c          499 g          581 t
ORIGIN      
        1 ttacattgtg ctcaatctgg gcatctttct catggcctgg tttcgggcgt ggcgcatcct
       61 caattatctg gcgttcaaat tcacgttcgg tgtggccgta gtgtggttgc tcacgcgtta
      121 tgagccggtc ttcttattgt catcagagat gttttttacc gccttctttg ttttgttttt
      181 tatcatcagt attgtctacg ctgtcaaaca gccggtcaaa ctcaaggggt tggtggatgg
      241 gtctctggtg tttgggttgc cgattgtcta tttttacatg cagtcagttt tgctcaaacc
      301 gtatgagtac ggtttggcct atctggcggc gatcatggtg gtggtgtatt ttgggacagc
      361 ctttgtcttg cgtaaaatgt ttcaagacag gatggaggcg ctgatccagt cgtattatgc
      421 tattggtgtg gtgtttatca ctttggtggg acctttggcg ttgtccgccc agtggacatc
      481 cgcgtattgg gccttgcagg gcgctggact ggtttggatc ggcgtcaggc aagcacgcac
      541 tctggccaga ttttctggcc tggctttgat cgtggtcgca ggattttctt tcacgtctct
      601 ttttgaggga aagcctgttt cactggatta cgtgattcca gtcctgaacg cggatttctt
      661 gagttcagtg atcaaggcac tcacagcctt gtacgccggg ctctatctga ggcggcaaac
      721 gaacgacaag cttttccatg cggaggctga aaaaggcatt gccgcgttcg ttgtgcttta
      781 tggattgtgc tggtggtact tgggtttggg tcgcgaaatc atgttgcacg cacctctggg
      841 atcccaaaat ctgttggcac tggtcctagt catcggttcg tatctggttt taaagccttt
      901 ggctcgcgtg acccaggatt cttttttgaa acaattcaat ctcattacgg ccgctgtggc
      961 cgcgcccatt atccttgaga gatactggat cagcctggcc tggtcgtgtt tgtccgttga
     1021 ccttgtctgt cagctcaagg ttatgtatgg ggacgccgcg aaaaaagatt cggacctggt
     1081 tttccgtcac gtcattttgt tgctgctgat ggttctgttt aacggcattt tgtttttcca
     1141 ctggcagtcg catcccgcgt tgatcagtct tggcaatctg agcgtttgcg tgtttgctgt
     1201 gagcgccttt tttgtggcct gggttttgta ttctgaaacg tctaagatga gaggcgatct
     1261 gatctcccac gttatgatcc tgtttgtttt tggtgtgttt tggtttgtgg gcataaacta
     1321 cattgatcaa gccgtcagct acgtgtttgc gggcaaggat caggttatca tgatctttat
     1381 gctgagcaca ctcgtattgt ctcaggctct gcatgcgagg ctctattgga aagcggccac
     1441 ttttccgatt caagtggcca ttttaccgct cacggcagtt gtttttgttt gggattggac
     1501 caacgcgcac ccggcgggag atttgggttg ggtcacgcat attttattga ttggtctcat
     1561 ggcttggtcc tgctggttgt ggcaacatca tgcaaaaaaa ccgctggagg aagatcttat
     1621 cacggcaccc ttcactaagg ttgtgcactg gtttggtgtg ttgttcatga tcagcgcagt
     1681 gactctcgag gcgcagtggt tggcaaaggg cctggtgcct gcgcaccccg tgtggtatga
     1741 tctggtgttc tgcctgatcc cagcgggttt tctgg
//
LOCUS       DATDUN010000151         4440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_422601, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000151 DATDUN010000000
VERSION     DATDUN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422601"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VJC18_02040"
     CDS             complement(<1..193)
                     /locus_tag="VJC18_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HLD44189.1"
                     /translation="MSQKLTLTKSDQLYKEAWELIPGAVAGIRRPYNFVPGEYPIYFK
                     TAKGGRVTDVDGNEYIDMLC"
     gene            585..1997
                     /locus_tag="VJC18_02045"
     CDS             585..1997
                     /locus_tag="VJC18_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLD44190.1"
                     /translation="MEPKLLQIYNKQKQNSLKIAKTSADARIHKLKKIRTAILEKRTD
                     IQRAIHADFQKHPLETDLTEIYPVLSEISHAIRHLKAWMKPKAVGTPLLLYGTKSEIR
                     YEPKGVVLILSPWNYPFQLCLIPLMTALAAGNTVIIKPSSKVPRTATFLQKFVSELFE
                     EDEVAVLTGDADVADALLELPFDHIFFTGSIPVGKKVMAAAAKHLTPVTLELGGKSPV
                     IIHESADIQKTAERVMWGKFVNAGQTCVAADYAFVHKSVHDDFVSACQKIITTRYGKN
                     ANEQKLSPDYCRLVSRASLGHLKKLLDESLAKGAKLVMGGHADVNDRYLCPTMITNVT
                     LDSPLMQEEIFGPILPLITFDKLDEVYDIIRSRPRPLALYVFAKDNHFIETILNNTSS
                     GGACINTLMVHLANNELPFGGIGYSGMGNYHGHFGFRTFSHERAVLRQGCLDTLKHFY
                     PPYTERVKQKIDWALKFVIR"
     gene            complement(1977..2204)
                     /locus_tag="VJC18_02050"
     CDS             complement(1977..2204)
                     /locus_tag="VJC18_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44191.1"
                     /translation="MEEKKEQAPPTADKICHVMRNRLSSLAAYIFVSSKYLQQETERE
                     LHITAQTSYAELRELVDEVERLLRGLSNDEF"
     gene            2368..2751
                     /locus_tag="VJC18_02055"
     CDS             2368..2751
                     /locus_tag="VJC18_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44192.1"
                     /translation="MLYIRRDKNIGSNIYLEIDPGITLKLRKQSTTGQERIVNIESKR
                     VKYERDIRFYHPGIDTQRLPLCPVSVEWIDEGGETGFYLLSFSEPAYFYVWKGGHQEL
                     RQPTMLDVYPDGRLRYHLQDGTTCD"
     gene            complement(2834..3997)
                     /locus_tag="VJC18_02060"
     CDS             complement(2834..3997)
                     /locus_tag="VJC18_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44193.1"
                     /translation="MEFQRTLKLDELLKKKSFFLFGPRSTGKTFLINKQFSSDTPVIS
                     LLDSEYFLRLSQEPQLLEQILLVNGSMPDTVVLDEIQKIPQLLSEVHRLLDKYKQKTH
                     FLLTGSSARKLRKHESDMLAGRAWTANLFPLTTAEIADFQLERYLRYGGLPQVWMSEE
                     PQEELVAYANTYLKEEITAEGLVRKLAPFARFLKTAALCNGQLMNFSQVASDAAVSAS
                     TIKDYFTILEDTLLGFMLEPWIFSKKRKAIQTAKFYFFDTGVAHTLADTQHIDRNSNL
                     YGTSFEHFIGLELRAYLSYSRTHLPLTFWRTTHDHEVDFLVGEQMAVEVKATTRVSSH
                     HLKGLMALKEEGVFKSHYIVSQDTVLQNIDGIISMHWREFLNRLWRGELITKT"
BASE COUNT         1254 a         1052 c          970 g         1164 t
ORIGIN      
        1 cgcagagcat atcgatgtat tcattgccat cgacatccgt gacacgccca cccttggctg
       61 ttttgaaata aataggatat tctccgggca caaagttgta gggccgacga atcccggcca
      121 ccgctcccgg aatgagttcc caggcctctt tgtacaattg atctgatttg gttaaagtga
      181 gtttttgtga catgtggaat cagtagctta cttgctttga tcgaatcaaa ttgttttttc
      241 ggagagtaag acattcgtca gtgaacagag cgttttgttt ttttgggtga tacggggagc
      301 tattattttg atgcggcctg cggagcaagc cccgcttgcg aagagtgatt aagtgtgggc
      361 gcgcgtaagc aagcccaagt aaacgcgata gccacgtgaa aacacaaagc aaagctttgt
      421 gccgctattg taaaatagcg acccgaagca ggacccgaaa aatcaaaacg ctccaaagag
      481 tgaccgatta cggagagtaa gacattcggc gctgtgtgaa aaatatccct tgcactccct
      541 gcacaatgat aagagactgc gacacaaact gatacggaga acttatggaa cccaaactcc
      601 tgcaaattta caacaaacaa aaacaaaact ccctgaagat agccaagacc agcgctgacg
      661 caagaatcca taaactcaaa aaaatcagaa ccgccattct ggagaagcga acagacatcc
      721 aacgggccat tcacgcagac tttcaaaaac atcccctcga aacagattta acagagattt
      781 atcctgtgct ttccgaaatc agtcacgcca tccggcacct taaagcctgg atgaaaccaa
      841 aggctgtcgg aacacccctg ttgttgtacg gtaccaagag cgaaatccgg tacgaaccaa
      901 aaggggtggt gctcattctg tccccctgga attatccttt tcaactctgt ctcatccctc
      961 tcatgacagc cctggccgct ggtaacacag tgatcatcaa gccctcaagc aaggtgccac
     1021 gcaccgccac ttttttgcaa aaatttgtga gtgagctatt tgaggaagat gaggtggccg
     1081 tgctaacagg cgatgcagac gtggcagatg ccttgttgga attgcctttc gatcatatct
     1141 tctttaccgg atctatcccc gtgggtaaaa aggtcatggc ggcggcggcg aaacacctca
     1201 caccagtcac tttggagttg ggcggcaaat cacctgtcat cattcatgag tcggcagaca
     1261 ttcaaaaaac agcggaacga gtcatgtggg gcaaattcgt caacgcaggg caaacctgcg
     1321 tggcagccga ttacgcattt gtccataaat cggtgcacga tgattttgtc tccgcctgcc
     1381 aaaaaatcat caccacgcgt tatggcaaaa atgccaacga acaaaaactg agtcccgatt
     1441 attgtcgttt ggtaagccgg gcgagcctgg gacacctcaa aaaactgctc gatgaatctc
     1501 tggccaaggg agccaagctc gtcatgggcg gtcacgccga cgtgaacgac cgctatttgt
     1561 gtcccacaat gatcaccaat gtgaccctgg actctcccct catgcaggag gaaatctttg
     1621 gtcccatctt gcccctcatc acttttgata agctggatga ggtttatgac atcatccgat
     1681 cacgccccag accactcgct ttgtatgttt ttgccaaaga caatcatttt atcgaaacca
     1741 tcctgaataa cacatcctca ggtggcgcct gcatcaacac gttgatggta cacttggcca
     1801 ataacgaact gccgtttggc ggtatcgggt acagtgggat gggaaattat cacgggcatt
     1861 ttggtttcag gactttttcg cacgaacgcg ctgtcctcag acaaggatgc ttggacaccc
     1921 tcaaacattt ctaccctccc tacactgagc gggtgaaaca gaaaatcgac tgggcattaa
     1981 aattcgtcat tcgataaacc gcgtaacaaa cgctccacct catcgaccag ttctcgcagt
     2041 tcggcatagc ttgtctgggc cgttatgtgc aattcccgtt ctgtttcctg ttgcaggtac
     2101 tttgaggaca caaaaatgta ggcggccagc gaggaaagtc tgttccgcat cacgtgacaa
     2161 atcttgtcgg cagttggtgg tgcctgttct tttttttctt ccactgaaac ctccctttct
     2221 gtcagacacc ctctacaaaa gacggtaatt tgtctacaat ggccttgtgt atcagaaaag
     2281 gcaagatttt tacacgttct gtcttctgat caatcaccac acatcgctgg agcaaccata
     2341 ctgacaggca cggttaacga aaactatgtg ttatatattc gtagagataa aaatatagga
     2401 tcaaatattt atttagaaat tgatcctggg atcacattaa aattgaggaa acaaagcaca
     2461 acaggccaag aacgtattgt aaatattgaa tctaaacgcg taaagtatga aagagatatc
     2521 agattttatc accctggcat agatacgcaa agactgccct tatgccctgt ttctgtagag
     2581 tggattgatg aaggcggcga aacaggcttt tatcttttgt cattttctga gcctgcttat
     2641 ttttacgtct ggaaaggagg tcatcaagag ctgagacaac caacaatgtt ggatgtatat
     2701 ccggacggcc gattacgata ccatcttcaa gatggcacca catgcgatta gatgattgaa
     2761 gaaagtaaaa aataaatgtt tgaccatatt tctgtttcaa ttcaataaaa cacataattc
     2821 ctgttctgca ccatcacgtt tttgtgatca attccccgcg ccacaaccga ttcaaaaact
     2881 cacgccaatg catgctgata atcccatcaa tgttttggag tactgtgtcc tgcgatacaa
     2941 tgtaatgtga tttaaaaacg ccttcttctt tcagcgccat cagccccttg aggtgatgag
     3001 atgacacccg ggtcgtggct ttaacctcaa cagccatttg ttcaccaaca agaaaatcaa
     3061 cttcgtgatc gtgcgttgtt ctccagaaag tgagcggtaa atgtgtgcgg ctgtagctca
     3121 aataggcccg caactccaag ccaatgaaat gttcaaaact ggtgccgtag agattggagt
     3181 tacgatcgat gtgctgcgta tccgccaaag tgtgcgccac gcccgtatca aaaaaataga
     3241 atttggccgt ttgaatcgct tttctttttt tggaaaatat ccacggctcc agcatgaatc
     3301 caagcagcgt gtcttcaaga atggtaaaat agtctttgat tgttgaggct gaaactgcag
     3361 catcgctagc cacctgagaa aaattcatca actgcccgtt acaaagcgcc gctgttttca
     3421 aaaaacgcgc aaagggggca agttttctga ccagtccctc tgccgtaatt tcctctttga
     3481 gatacgtgtt cgcataggca accagctctt cctgtggttc ttcactcatc cagacttgcg
     3541 gaagccctcc ataacgcagg taacgctcaa gctgaaaatc tgcaatctca gctgttgtga
     3601 gaggaaacag attggcagtc caggcgcgac ctgccaacat gtcagattcg tgttttctga
     3661 gtttgcgggc actgcttccc gtcaacagaa aatgggtttt ttgtttatac ttatctaata
     3721 acctgtgaac ttcagataac agttgaggga ttttttgaat ttcgtccaac acgacagtgt
     3781 caggcatcga tccattaacc agtaaaattt gttccagcag ttgtggttcc tgtgacagac
     3841 gcaaaaaata ttcactatcc agaagtgaaa tgaccggcgt gtctgacgaa aattgtttgt
     3901 tgatgagaaa agttttccct gtagatcggg gaccaaatag aaaaaaagat ttttttttga
     3961 gcaattcgtc aagtttgagt gtgcgttgaa attccataca cgtatcttac caaaacgctt
     4021 tcggataaac aacaccaatt ctgttgttta tccgtatata caacggtagc actgttgatt
     4081 atcccaacac atcggctgaa tcgacagaac acaaacaact ctatctaatt gattatatta
     4141 gttttttcat atttcatcaa ataatgtaat gcaaaatatc tcagaatgga tacggggtca
     4201 aagattgaca agagacaagt tgcaagttgg gctggcaaga cctttttcat gaacatgtct
     4261 cttgtcaatc tttgacccca gccttacgat aagccgcgta acatccgctc cacctcatcg
     4321 accagttctc gcagttcggc atagcttgtc tgggccgtta tgtgcaattc ccgttctgtt
     4381 tcctgttgca ggtactttga ggacacaaaa atgtaggcgg ccagcgagga aagtctgttc
//
LOCUS       DATDUN010000152         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_91523, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000152 DATDUN010000000
VERSION     DATDUN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91523"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VJC18_02065"
     CDS             <1..944
                     /locus_tag="VJC18_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44194.1"
                     /translation="NKLAIYLNSSPLKKLGERTLDHYLKTYFNMSDSLQKGELLPSTT
                     PTQRGELAKNRFTTAGFLIMSLIERAEFEAHKKEITLLADWLLAQQRPDGIFRTQFAD
                     SQYFDSGQLLLAIATLYEKTREKKYKDFFDKSFEAYASQLKQQMSFGGKYYTAYAPAW
                     FTQPFAKMYFITQETKYRDLVFALNDPLLHWYEIYADHPVYFDYDGVLVPKTGSYGNN
                     SVTSAALESLVDAALVARTSADTQRYQQYTHVIKQTVAHLMRLQYTPANTYYIGNKRT
                     VTGGFKSDLIDSKLWMDNVWHLTSAFIKIHQNKLLPD"
     gene            complement(1024..>2370)
                     /locus_tag="VJC18_02070"
     CDS             complement(1024..>2370)
                     /locus_tag="VJC18_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44195.1"
                     /translation="EDFDETTSFAACQMFNMTKTAISQASLGDQMLCHIQNGLEFEAT
                     KEGVDIADGKSHVLALDFSQDPYQEDEGKGMPDLIRIKIEKNAEGAITFFEMAACAGD
                     EQAMFVSQQVEASQFEMKAINIGSGEDMQYQDSVIVTGTLNADGFFVDEVDGEETPKL
                     IEMQHKSKGAGASEGSFDFWGNLIFKQFSDLAMMKGQMSGANKWEDEGCEFTDQIVAH
                     MDLIDGNGEAIAKEDYQISLLELGDGALLGMSSGGCGDKTWEHEYTEAWTGADALVLE
                     PYTDSGHYNIIKDQALPESSSPDISFVEDQIFDCSSTPEATLVFGAGGGGSQAKQLTA
                     GEEPKSGEMCQNFMLNHEYIDCWHQVEEVKEDLEQGGGGKFGEGEPLDLAVCGGEPDE
                     SDSISEKETVDALCECFEWDEETCTSKIDECKDAEGVVTAGDCVDYINGEGGDDPA"
BASE COUNT          606 a          697 c          473 g          594 t
ORIGIN      
        1 taaacaagct ggccatctat ctaaacagtt cccctttaaa gaaactgggg gagcgcaccc
       61 tggaccatta tctgaaaacc tatttcaaca tgagcgattc cctgcaaaag ggtgaactcc
      121 tgccttctac gacccccacc cagagaggag aactggcgaa gaacagattc accacagcag
      181 gcttcctcat catgtcgctg atcgagcgcg ctgaatttga agcgcacaaa aaagaaatca
      241 ccctgctggc agattggctc ctggcacagc agagaccaga cggcattttt cggacgcaat
      301 tcgccgacag tcaatatttc gattcggggc agttgttgct cgccatcgcc actctgtatg
      361 agaaaaccag agaaaaaaaa tacaaggatt tttttgacaa gtcttttgag gcctacgcca
      421 gccagctcaa acaacagatg tcttttggtg gcaaatacta caccgcatac gctcccgcct
      481 ggttcaccca accttttgcc aaaatgtatt tcattacgca ggagaccaaa taccgcgacc
      541 tggtttttgc cctgaacgac cccctcctcc actggtacga aatttatgca gaccaccccg
      601 tgtattttga ttacgatggg gtgctggtcc ccaagacagg gagctatggg aacaactctg
      661 tcacttctgc tgccctggaa tcattggttg atgcagcttt ggtggcaaga acatccgcgg
      721 atacccaacg ctaccagcag tacacacacg tgatcaaaca aaccgtcgct cacttgatgc
      781 gtctgcaata cacacctgcc aacacctatt acatcggcaa taaacgcact gtcacgggag
      841 ggttcaagtc ggatctcatt gattcaaagt tatggatgga taacgtctgg cacctcacca
      901 gcgcgttcat caagattcat cagaataaac tgctaccaga ctgatgtgtc atggaccaga
      961 gagccaacgg catggggcgc gcaagaatga caaacttcag atcgtggtct tgtaacgcag
     1021 gggttacgcg ggatcgtctc ccccttctcc attgatataa tctacacaat cgccggctgt
     1081 cacgacaccc tcggcatctt tacactcgtc tattttgctt gtgcatgttt cttcatccca
     1141 ttcaaaacac tcgcaaagtg catcgactgt ttccttctca gaaatcgaat cactctcgtc
     1201 gggttctccg ccacaaaccg ccagatccag cggttctcct tccccaaact tgccaccacc
     1261 tccctgttca aggtcctcct tgacttcttc cacttgatgc cagcaatcga tgtactcatg
     1321 attgagcata aagttctggc acatctcccc actctttggt tcttcgccag cggtcagctg
     1381 ttttgcctgc gaaccaccac cacctgcgcc aaacaccagc gtggcctcag gtgtgctgga
     1441 acaatcaaaa atctggtctt caacgaaaga aatgtctggc gacgaactct ctggcaacgc
     1501 ctgatcttta atgatattgt aatgcccact atcggtgtag ggctctaata ccaacgcatc
     1561 cgcccctgtc caagcctcag tatattcatg ttcccaagtc ttgtcaccac aaccaccact
     1621 gctcatgccc aacaaggcgc catcacccaa ttccagcaga ctaatttgat aatcttcctt
     1681 ggcaatggcc tctccattgc cgtcgatcaa atccatatgg gccacaatct gatcggtgaa
     1741 ttcacacccc tcatcttccc acttattcgc tccactcatc tgacctttca tcatggccaa
     1801 atcagagaac tgcttaaaaa tcagattgcc ccagaaatca aacgagcctt ccgaagcacc
     1861 agcccctttt gatttgtgtt gcatctcaat caatttggga gtttcctcac catcgacctc
     1921 atccacgaaa aagccatccg cgttgagggt tcccgtcaca atcacggaat cctgatactg
     1981 catgtcctct cctgatccga tgttgatggc tttcatctcg aactgactcg cctcgacttg
     2041 ctgactaaca aacatcgctt gttcatcacc tgcacaggcc gccatctcga aaaaagtgat
     2101 cgcgccttca gcgtttttct cgatcttgat gcgaatgagg tcgggcattc ccttgccctc
     2161 gtcttcctga taagggtctt gtgaaaaatc cagcgccaaa acgtgagact ttccgtccgc
     2221 gatgtcaaca ccctcttttg tagcctcgaa ctccaatccg ttttgtatat ggcacaacat
     2281 ttgatcgcca agactggcct gagaaatggc tgtcttggtc atattaaaca tctgacaagc
     2341 ggcgaaactg gttgtttcat caaaatcttc
//
LOCUS       DATDUN010000153         2772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_341130, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000153 DATDUN010000000
VERSION     DATDUN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341130"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VJC18_02075"
     CDS             complement(<1..1054)
                     /locus_tag="VJC18_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HLD44196.1"
                     /translation="MDVLFLSRLQFAVATLFHFIFVPLTLGLSIIVAIFETRYARTGD
                     ELYLKLTKFWGKLFLINFALGVVTGITLEFQFGTNWSRYSAFVGDIFGSLLAIEATVA
                     FFLESTLVGVWIFGWQKLSPKKHALIMWLIAVAGTLSAVWIILANGWMQNPVGYVIKN
                     GRAELASFSAVFFNKYAWLEFFHTMSASYLYSAFFVMGVSAYHLIKKQHVDFFTRSFK
                     LALWFGLVCSVLVAFSGDLNGVNVAKVQPAKLAAMESHWQTSANAPMKLLVWPDEEAG
                     KNAFEAFSVPSGLSLLAFHDPHATVKGLRDFAQEDRPPVLITFVAFRVMVAIGGLLIL
                     LCVIGFFRRKRLVDSKW"
     gene            1339..>2772
                     /locus_tag="VJC18_02080"
     CDS             1339..>2772
                     /locus_tag="VJC18_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44197.1"
                     /translation="MNTATTALRAPLAQPVAPNVFILSTGNPTRGKATQSLASRYAAF
                     AGATAQTAHLTGRQTRKPAAQRLAAPATPSSDAPQSTDRPGGTGGSDNTGPIGKPYGY
                     VQKMRRGGVAVYTSQGWVQLGLPMGSHLDAIQMFFKNGGQPGKTPSDKLGHYMPLHYV
                     PVKGYTTFTTGYHAVDFTRYPYFWPLLQQAGFNFYLPTPGDAGKVSAFLKEAFQGRNP
                     QKLTDSVRDEHASNEEGIPDLGTETQRFANKASEDFSRVTHFENDEITIGDGSVVVKQ
                     AQEKGPMAYEIYDRGHLLRTIDLGKPEFAPPVENLDVDPRTMFDPKFQEIRKRVLIDG
                     EAGLWPISTADYFSGEGTSGFMLWKDGKVTLIDPPAQTVAYLIQNGIPLNAVEGIAIT
                     HDHTDHFTPASIELMFLCPKLNWYGTKTLYNICQDAYTLSIAVNGKPEGLTQLNFVEL
                     KPRQFVAVRGMWLRTHYTCHSVAAEAIE"
BASE COUNT          781 a          771 c          657 g          563 t
ORIGIN      
        1 accatttgga atcgaccaac cgtttgcgcc tgaaaaaccc gatcacacaa agcagaatca
       61 gcaagccccc aatggccacc atgaccctga acgccacaaa ggtgatgagc acaggcggac
      121 gatcctcctg ggcaaaatcg cgcaaccctt tcactgtggc atgaggatcg tgaaacgcca
      181 gcaaactcaa tcccgacggc accgaaaagg cttcaaatgc atttttaccg gcctcttcat
      241 cgggccaaac cagaagcttc atgggcgcgt tggcgctcgt ttgccagtgt gattccatgg
      301 cggcgagttt ggcaggttgg actttggcca cgttcacgcc gttcaggtca ccgctaaagg
      361 ccaccaaaac cgaacacacc agaccaaacc agagggccaa tttgaaagaa cgtgtgaaaa
      421 aatcgacatg ttgtttttta atgagatgat acgcgctcac tcccatcaca aaaaaggcgc
      481 tgtataaata cgacgcactc atggtgtgaa aaaactccag ccacgcgtac ttgttaaaaa
      541 acacggcact aaaacttgcc agctctgccc gtccattttt gatcacgtaa cccactggat
      601 tctgcatcca cccattggcc agaatgatcc acaccgccga gagcgtgccc gccacagcaa
      661 tgagccacat gatgagcgcg tgctttttgg gcgagagttt ttgccagcca aaaatccaca
      721 cccccacgag tgtggattcc aaaaaaaacg cgaccgtggc ctcgatggcc aacagtgatc
      781 caaagatatc gcccacaaag gccgaataac gggaccagtt ggtcccaaac tgaaactcca
      841 gtgtgattcc cgtcaccacc cccaaggcaa aattgatgag aaaaagtttg ccccagaatt
      901 tggtgagctt gagataaagc tcgtcaccag ttctggcata gcgtgtttcg aaaatcgcca
      961 caatgatcga gaggcccaaa gtcagtggga caaagataaa atgaaacagg gtggccacgg
     1021 caaattgcag gcgggacaaa aaaaggacat ccatgcccag ccataaaaca gaaaacctgg
     1081 ccttgttcaa tgaaaatgaa ttagtgataa gtcaccgtca tcgcgagcgg agccatgagc
     1141 catttacagg ctcatggcga agcgtggcga tccacaagaa acaatatgga gatcaccacg
     1201 tcgtccgcct gcggcgacct actcgtgatg acatgacacc atttcctgac gcatgccccc
     1261 tgtctacaag aaaattttga caccagacac caaaagcact atgcggctgg tatcatcaca
     1321 taaaatgggg aggacagcat gaacacagca accaccgcct taagagcccc actcgcacaa
     1381 cccgtcgcac caaacgtttt catcctttcc acaggcaatc caactcgcgg taaggcaaca
     1441 caaagcttgg cgagtcgtta tgccgccttt gctggtgcaa cggcccaaac tgcacatttg
     1501 acgggaaggc aaacaagaaa acctgcagcg caaaggcttg ccgcacctgc aacaccaagc
     1561 tcggacgccc cacaatccac tgatcgcccg ggtggaacag gcggttccga taacacaggc
     1621 cccattggca aaccatacgg ttatgtgcaa aaaatgaggc gcggtggcgt tgccgtttac
     1681 acgtctcaag gttgggtaca gttggggctc cccatgggat cgcacctcga tgccattcag
     1741 atgttcttta aaaacggcgg acaacccggc aaaacaccct ctgataaact cggtcattac
     1801 atgcccctgc actatgtgcc tgtcaaaggt tacacgacat ttacaacggg ttatcacgcg
     1861 gtagacttta cccgttatcc ctacttttgg ccgctcttgc aacaagctgg gtttaatttt
     1921 tatttgccaa ccccgggtga cgcagggaaa gtcagtgcct ttttgaaaga ggcctttcag
     1981 ggacgcaacc cacaaaaact gaccgacagt gtacgagacg aacatgcttc caatgaagag
     2041 ggcattcccg atttgggcac tgaaacacag cgtttcgcca acaaggcatc cgaggacttt
     2101 tccagagtca cccattttga gaacgacgaa atcacgatcg gggatggtag cgttgtcgtc
     2161 aaacaggctc aggaaaaagg acccatggcg tacgaaattt atgatcgcgg gcatttgcta
     2221 cgcactatcg atctgggcaa accggaattc gccccgcccg tggaaaatct cgatgttgat
     2281 cccagaacca tgttcgatcc caagtttcag gaaatccgca aacgggtctt gatagacggc
     2341 gaagcaggtc tttggcccat cagcacagcg gattattttt cgggcgaggg caccagcggg
     2401 ttcatgcttt ggaaggatgg caaggttacg ctgatcgacc ccccggcaca gacggttgct
     2461 tatctcatac aaaacgggat tcccttgaac gcggtggagg gcatcgccat cacacatgat
     2521 cacaccgatc atttcactcc tgccagtata gaactcatgt tcctgtgccc aaaactcaat
     2581 tggtacggaa caaaaacctt gtacaatatt tgtcaggatg catacaccct aagcatcgcc
     2641 gttaatggca aacccgaggg tttgactcaa ttgaattttg tggaactcaa acctcgacag
     2701 ttcgttgcgg tccggggcat gtggctcaga acccactaca cctgtcattc tgtggccgcc
     2761 gaggccattg aa
//
LOCUS       DATDUN010000154         1245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_505315, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000154 DATDUN010000000
VERSION     DATDUN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505315"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..>1245
                     /locus_tag="VJC18_02085"
     CDS             288..>1245
                     /locus_tag="VJC18_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLD44198.1"
                     /translation="MTQSQFQFIICRAKFVLPLRANNLNERITDGYLLSEGEHIREVG
                     RFSRDVENRMRTTYGTRMGVLCAQGIEPLETLKDIPCHNAVLMPAFVKAHGHDHEQPL
                     IGIAKDEPLTSWLDHAVNPFTGFMNKQHDELCRVLGDSPQKITYRMARLCDIHYGITT
                     SMVHHCNHNKYHVREIAEVNETAGTTMIVAVGGQDRFYVKELLDDPRDAVARMDQALQ
                     ITGLQHTTFCPGPDQVFSNSRKMIVPLKNWAREHGTLFHIHSAEEPKTTAWFKEAIEP
                     GMTEVEFLEAIGVLDSETVLAHQVNCFGRDVEILARHQTRIVH"
BASE COUNT          328 a          295 c          350 g          272 t
ORIGIN      
        1 cgtgcggtgt gtggttgcgg acgtggccgt tcagcgcgga aaaggttttg agggcgttgg
       61 ggaggatgtg agaatatcga atatcgaata tcgaatcctt caacttcgtt caggacaggt
      121 atcgaacaag gaacagggaa caaggaatgg cgaaaaatgt caatgcctgc gaaggcaggc
      181 atccaaaagt ttgtcatccc tgcgaaggca gggatccaga atgtatgtac acatggattc
      241 cagcctacgc tggaatgaca agatgtgcgt caagggggta aattagtatg acacaatcac
      301 aatttcaatt catcatctgc cgagcaaaat ttgtgttgcc actcagggca aataacctca
      361 acgaacgcat caccgacggg tatcttttgt cggagggaga acacatcaga gaggtagggc
      421 gtttctcaag agatgtcgaa aacagaatgc ggacaacgta cggcacgcgt atgggtgtgc
      481 tttgtgccca gggtatcgaa ccattagaaa cgctcaaaga cattccttgt cataacgcgg
      541 ttctcatgcc ggcctttgtc aaggcgcatg ggcacgatca cgaacagccg ctcatcggca
      601 tcgccaagga tgaacccctc accagttggc tggatcatgc ggtcaatccc tttacgggtt
      661 ttatgaacaa acagcacgat gagctgtgtc gcgtgctggg ggattctccg caaaaaatca
      721 cctaccgcat ggcaaggctt tgtgacattc attatggcat caccaccagc atggtgcacc
      781 attgcaatca caacaagtat catgtccgcg aaatcgctga agtcaacgag actgccggca
      841 ccaccatgat tgtggcggtg ggcgggcagg atcgttttta cgtcaaggaa ctgctggatg
      901 acccgcggga cgctgtagcg cgcatggatc aggctttgca gatcaccggc ctccagcaca
      961 ccacgttttg tccgggacca gatcaggtgt tttccaattc acgcaagatg attgtgccgc
     1021 tcaaaaactg ggccagagag cacgggacgt tgtttcacat ccacagtgcc gaggaaccaa
     1081 agaccacggc gtggtttaag gaggccattg aacccggaat gaccgaagtg gagtttctcg
     1141 aggccatcgg tgttttggat agcgagacag tgctcgcgca tcaggtgaat tgttttgggc
     1201 gcgatgtaga aatattggcc cgtcatcaaa cgaggattgt gcaca
//
LOCUS       DATDUN010000155         1673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_588981, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000155 DATDUN010000000
VERSION     DATDUN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588981"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VJC18_02090"
     CDS             <1..410
                     /locus_tag="VJC18_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLD44199.1"
                     /translation="IGERTAELVKINIGNAFPGGEVRTMEISGRDLVSGIPRHLEINS
                     DEIREAIAEPIRIIIEAIKATLEQTPPELAGDIVDKGIMLAGGGALIRDIDKLIHEET
                     GLPVMIADDPLSCVAHGAGKALDELSVLREVSS"
     gene            668..1519
                     /gene="mreC"
                     /locus_tag="VJC18_02095"
     CDS             668..1519
                     /gene="mreC"
                     /locus_tag="VJC18_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HLD44200.1"
                     /translation="MRKIFLLFIVIVLVLLLVHFSISRETFFSRQFITDNVVTYVFEP
                     VVSQVKNVKQYFANLFNDYIVLVGVKRENKQLSQTVKVLALQNQSLKDELQRQYDVVV
                     AKEQFQYFNYDLLNVNLLGFDPFLLSKTAWIDAGQDKNIKPDYVLVTTDGLVGRVIRV
                     YDKTSTVLLLIDRDFAVDAVTQDTRTRCLVRGLNLSQIQAKHLPFLSQVEFVEDKLLL
                     DKGDELITSGLSHIYPGGISVGRVVDVLLGEAGGWDTSYVMPSVDFAKLNQVFVLVPR
                     SDQPEAQ"
BASE COUNT          394 a          360 c          434 g          485 t
ORIGIN      
        1 tcattggtga acgcacggct gaactggtca aaatcaacat tggcaacgcg tttcccggtg
       61 gtgaggtgcg caccatggaa atcagcggtc gcgatttggt gagcggtatc ccccgtcatc
      121 tcgagatcaa ctccgacgaa attcgtgagg cgatcgccga gcccatccgt atcatcattg
      181 aggccatcaa ggccacgctc gagcaaacgc cacctgagtt ggcgggtgac attgtcgata
      241 agggcatcat gttggccggt ggtggtgccc tcattcgcga tatcgacaag ctcattcacg
      301 aagagacagg tcttcccgtc atgatcgctg atgaccctct ttcctgtgtg gctcatggcg
      361 cgggtaaggc tttggatgag ttgagtgtgc tcagagaagt ctccagttag gataatcgta
      421 gaaatcgctg aatctctgcg ttattccgta gttgctgatt cttgattccg caactgcgga
      481 atcaagaaaa ccgttcaaat cgttgaatat caaggcgctc agggattttt aaaccggaat
      541 gtatatggaa atacatgagg atttaaaaat tcctgagcaa cgcggagatt cagcgatttc
      601 aacggtttag cctaaatgga gcaatgtcaa ttgcggaatt taggctgatt ctaactcgta
      661 ttcgttcatg agaaaaatct ttcttttatt cattgtcatt gttctggttc tgttgctggt
      721 gcatttttcg atcagtcgcg agactttttt ttcgcgtcaa ttcattactg ataacgttgt
      781 gacctatgtg tttgagcccg tggtttctca agtcaaaaat gtgaaacagt atttcgcgaa
      841 tctgtttaac gactacatcg tacttgtggg cgtgaagagg gagaacaaac agctgtccca
      901 gacggtgaag gttctggccc tgcaaaatca gtctctcaag gatgaactgc aaaggcagta
      961 cgatgttgtg gtggccaagg agcaatttca gtatttcaat tatgatttac tcaacgtgaa
     1021 tttattgggt ttcgacccct ttctgctctc caaaacagcc tggatcgatg ccggtcagga
     1081 taaaaatatc aaaccagatt acgttctggt cacaactgac ggactggtcg gtcgcgtgat
     1141 acgcgtgtac gataaaacca gcaccgtatt actgctcatc gatcgtgatt ttgcggttga
     1201 tgcagtgacc caggacacgc gcacccgatg tcttgtgcgc ggattgaacc tttcgcaaat
     1261 tcaggccaaa cacctgcctt ttttgagtca agtcgaattt gtcgaggaca agttgttgtt
     1321 ggacaaaggt gatgagctca tcacgtctgg tttgagtcac atctatccgg ggggcatttc
     1381 tgtgggacgt gtggtggatg tcttactggg agaggcgggt ggttgggata cgagttatgt
     1441 catgccttcg gttgattttg ccaagctgaa tcaggttttt gtactggttc cgcgcagtga
     1501 ccagccggaa gcacagtgat tcaaaaatgc tgatattatc tttgatcatc atgagtgtgg
     1561 gtttggtctg tgatctcatc ctgcagattc cgattggctc tgtcgtggtc aaggcccagt
     1621 tttgtcacct gttcttgtat tatctgctgt ttcgcaggga ttttaaacac gtc
//
LOCUS       DATDUN010000156         1018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_505378, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000156 DATDUN010000000
VERSION     DATDUN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505378"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VJC18_02100"
     CDS             complement(<1..405)
                     /locus_tag="VJC18_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HLD44201.1"
                     /translation="MLEFAQSLRWQDIFDILIVSFIIYRIMLLIRGTRAVQMLAGIVV
                     LITLYFAAREFEFLTLYWLLGTFLSSIFLIIIIIFQRDIRRALTQVGQTTFSKPQEDM
                     VHILLEIVRAAQQMAQRKVGGLIVIERETGLND"
     gene            510..878
                     /locus_tag="VJC18_02105"
     CDS             510..878
                     /locus_tag="VJC18_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44202.1"
                     /translation="MNFFKKIEEIGHGLARRKAVFCAQDLSGLARKYSTQSAQLLLDK
                     VGDLIYLHKIFQHISVGVVRERRPGRKPASREFTWAECLESINNTVTGVENWLIISPP
                     KKGIVRISCNGFAIAPTRAR"
     gene            794..>1018
                     /gene="rpsT"
                     /locus_tag="VJC18_02110"
     CDS             794..>1018
                     /gene="rpsT"
                     /locus_tag="VJC18_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HLD44203.1"
                     /translation="MANHKSAEKRNRQNQLQRLRNRSNKSKMKGVVKKVLEAVELKAG
                     DQAQEALKLAIPIIEKTAVKGSIHKKNASRK"
BASE COUNT          279 a          216 c          295 g          228 t
ORIGIN      
        1 gtcgttgagt ccggtttccc gttcgatgac gatgaggccg ccgaccttgc gttgggccat
       61 ttgctgcgca gccctgacga tctcgagcaa aatgtgcacc atgtcttcct ggggcttgga
      121 aaaggtggtt tgcccaacct gggtgagggc gcgtcggata tcccgctgga agatgataat
      181 gatgatgagg aagatggagc taaggaacgt ccccagcagc cagtagaggg tcagaaattc
      241 gaattcccgt gcggcaaaat agagcgtgat gagaacaacg atgccagcca gcatttggac
      301 ggcgcgggtt ccccggatca gcagcatgat acgatagatg atgaacgaaa cgatcaggat
      361 atcgaagatg tcttgccagc ggagtgactg tgcgaattcg agcatcgggg tccgggttcc
      421 gtcaataaaa aatctggggg tgaaaaggtg aaactatctc atcttttttt aaagtaaacg
      481 aaaagcaggg tgccaggcaa ggggcatgaa tgaatttttt taaaaaaatt gaggagatcg
      541 gtcatggtct tgcgcgaaga aaggctgttt tttgtgcgca ggacttgtcg ggtctggccc
      601 gcaaatattc aacacagtct gcccagttgt tgcttgacaa ggtgggcgat ttgatttatc
      661 ttcacaaaat tttccagcat atttctgtcg gtgttgtccg ggaacggcgg ccgggcagaa
      721 aacctgccag ccgtgaattc acgtgggcag agtgtcttga atcaatcaac aatacagtaa
      781 caggagttga gaattggcta atcataagtc cgccgaaaaa aggaatcgtc agaatcagtt
      841 gcaacggctt cgcaatcgct ccaacaagag caagatgaag ggtgttgtca agaaggtcct
      901 tgaggccgtt gagctgaaag ccggggacca ggcccaggaa gcattgaagt tggcgatccc
      961 cattattgag aaaacagcgg taaaaggttc catccacaaa aagaatgcct ctcgcaag
//
LOCUS       DATDUN010000157         1274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_92078, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000157 DATDUN010000000
VERSION     DATDUN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92078"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /gene="secA"
                     /locus_tag="VJC18_02115"
     CDS             complement(<1..608)
                     /gene="secA"
                     /locus_tag="VJC18_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907895.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HLD44204.1"
                     /translation="MFKILQKIFGTANDRVIKRYQERVGHILSLEAAISKLTDDELKA
                     KTVYFKDKLAQGATLDDVLNESFAVVREVAKRTLDMRHFDVQILGGMVLHDGNIAEMK
                     TGEGKTLVATLPMYLNSLTGKGCHLVTVNDYLATRDAEWMGHVYKFLGLSVGVILHGL
                     NDAERRAAYRSDITYGTNNEFGFDYLRDNMKFSIQSMAQKKLS"
     gene            complement(630..>1274)
                     /locus_tag="VJC18_02120"
     CDS             complement(630..>1274)
                     /locus_tag="VJC18_02120"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HLD44205.1"
                     /translation="NEFISYPNRFDQIMFGSVEEAWQLGATAVGATIYFGSEESTRQI
                     QEVSQAFQLAHELGLVTVLWCYLRNPEFKKDKDYHVAADLTGQANHLGVTIEADIIKQ
                     KLPETNGGYNVLKFGKTHAKVYSELSSAHPIDLCRYQVLNCYAGRLGLINSGGASSGA
                     SDLAEAVKTAVINKRAGGMGLISGRKAFQRPMKEGVEILNAIQDVYLDKEISIA"
BASE COUNT          298 a          359 c          293 g          324 t
ORIGIN      
        1 gagagttttt tttgcgccat gctctgaatg gaaaatttca tgttgtcgcg caaataatcg
       61 aaaccgaatt cgttattggt cccgtacgtg atatctgagc gataggccgc gcggcgttct
      121 gcgtcgttca acccgtgcag aatcacaccc acagacagac ccagaaattt ataaacatgt
      181 cccatccatt cggcatcgcg tgtggccaaa taatcgttca ccgtgaccag atgacaaccc
      241 ttgcccgtaa gcgagttgag atacatcggc aaggtcgcga caagtgtttt gccctcacct
      301 gttttcattt cggcaatatt gccatcgtgg agcaccatgc cacccaatat ctgcacatcg
      361 aagtgccgca tgtcgagcgt ccttttggcc acctcgcgaa cgaccgcaaa actttcgttc
      421 aggacatcat cgagcgttgc cccttgcgcc agcttatcct tgaaatagac cgtcttggct
      481 ttcaactcgt catcggtcaa ttttgatatc gccgcttcca gagagagaat gtggcccacc
      541 cgttcctgat aacgcttgat gacacggtca ttggccgtac cgaatatttt ttgtagaatt
      601 ttaaacatag caaaaacacc ctcagccctt tatgctatcg agatttcttt gtccaaatag
      661 acatcctgaa tggcattcag gatctcaacc ccttctttca tcggtctctg aaaggctttg
      721 cgaccggaaa tcaggcccat cccgccagcc ctcttgttga tcacggccgt tttgaccgcc
      781 tcagccaaat cactcgcgcc gctggaggca ccacctgagt tgatcaggcc caatcgccca
      841 gcgtaacagt tgaggacctg gtaccggcac aaatcgatgg ggtgggcact ggagagctca
      901 gagtaaacct tggcatgtgt ttttccaaac ttgagcacat tgtaaccgcc attggtttcg
      961 ggcaacttct gtttgatgat gtccgcttcg atggtcacac ccaagtggtt cgcctgcccc
     1021 gtgaggtcgg ccgcgacatg gtagtctttg tcttttttga attcgggatt acgcagataa
     1081 caccacaaca cagtgaccag acccaactca tgcgccagct gaaaggcctg cgagacttcc
     1141 tgaatttggc gagtggattc ttcggaacca aaatagatag tggcccccac cgccgtcgcg
     1201 cccaattgcc aggcctcttc cacactgcca aacatgatct gatcgaacct gttggggtag
     1261 ctgatgaatt cgtt
//
LOCUS       DATDUN010000158         6997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_463913, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000158 DATDUN010000000
VERSION     DATDUN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463913"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VJC18_02125"
     CDS             <1..972
                     /locus_tag="VJC18_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HLD44206.1"
                     /translation="GICMAIGASYAGDLAFTTTSGPGLSLKSEALGLAIIAEIPLVVV
                     NVQRGGPSTGLPTKTEQSDLFQALYGRHGESPLPVLAASTPANCFDYTVTAAKIAVEY
                     MTPVILLTDGYLGNGSEPWKLPNLESLPIIKPREPASQQGEFLSYRRDPKTLAREWCE
                     PGTPGMAHRIGGLEKEDITGNVSYVPQNHEFMVRARDEKILRVVNDIPEQPVIGDQSG
                     DLLVVSWGGTYGAVLTAVSEMRTEGHRIGMAHFHHIYPLPKNTKNIFDQFKQIVVCEL
                     NLGQFHQHLSTNYPTKKLLKFNKVQGQPFMIAELKQCFAHLLSGGNS"
     gene            969..2006
                     /locus_tag="VJC18_02130"
     CDS             969..2006
                     /locus_tag="VJC18_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HLD44207.1"
                     /translation="MNTPQASEKTVTPLTKKDFMSDQEVRWCPGCGDYAVLACVQKVL
                     AELGANKDNVVFVSGIGCSSRFPYYMNTYGFHTIHGRATAVATGVKVANPKLDVWVIS
                     GDGDALSIGGNHFIHALRRNIGFKLLVLNNEIYGLTKGQYSPTTKQGSVTKSSPYGAV
                     DRTFSPAALGLGANATFYARAVDTNPKLLTEVILAAAQHRGTSFVEILQNCVIFNDKC
                     HHAITSKETRADNQLLLQHGKPMLFGANNNKGIILRGTELIVGTLGERGITEKDILIH
                     DAHAESPNLAFMLSQLNLPHAPVPLGIIRQISAPTYEDSVCEQIARVKASKGVGDFDK
                     LLHSGDTWEVG"
     gene            complement(2015..2332)
                     /locus_tag="VJC18_02135"
     CDS             complement(2015..2332)
                     /locus_tag="VJC18_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HLD44208.1"
                     /translation="MTPKKKRIKNSHLINFTFNNILYVAAVCVFVAFFSLTVWGDGGL
                     TELHALKAKRDGIIKQSHFLLRENLFFLQEMEELKKSRYIEQRVRTDLGYIRPDEIMY
                     VVK"
     gene            complement(2367..2780)
                     /locus_tag="VJC18_02140"
     CDS             complement(2367..2780)
                     /locus_tag="VJC18_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013182518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HLD44209.1"
                     /translation="MNPISQSKFDDVARRLEALGVTKEVLREEFIRGSGAGGQKINKT
                     NSCVQLTHTPSGIVIRCQHTRSREQNRFFARRILCERLEAIQLGKKSEKEKRLHRIRA
                     QKRRRSRRAKDKMLSSKKFQGEKKKQRKKPAENGE"
     gene            complement(2784..4481)
                     /gene="yidC"
                     /locus_tag="VJC18_02145"
     CDS             complement(2784..4481)
                     /gene="yidC"
                     /locus_tag="VJC18_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HLD44210.1"
                     /translation="MRQEFRLLSAVVLSTAVFVGYQMFFAPKPPQQPTQTTQQTAVPV
                     EPAQIIQSQQPQSLEIPQGESPSTGEIQAEEKTISVETPLAHITLSTRGAVITDYQLK
                     KFKLKAKKDSPPKNLLPEEGTSSALFLGFKGYSPLTAGKVYRVVSDQELPAGVRTIVL
                     EWQNDSVRIQKTFVFDGTQNNYTVTVAYEVANLSQSDLVLTPYLQNSITQKPIPKKTG
                     GLLSFLKFDRPDLYGLYHLADKTLTEDQNWQKFAGTREVAGNIYWSGIADRYFLFALL
                     PQDVQGPVKAQFGREKDNIVNQLYANELIVAPQQKKGGHFLAYVGPKQLSYLERVSSE
                     LKRAVDYGWFSIIAVLILKLMSFLHRFIPSWGLTIITLTFIVKMLLHPFNKKSMQSMK
                     AMQNLQPKLNEIKKKYGDDKQKQNEETMQLFRTHKVNPMGGCLPMLLQFPVYIALYKV
                     LWNAIELYHAPFLGIYADLSAPDPYFVSPILLGVFMFLQQKLTPTASVDPAQQKMMMF
                     MPVMFTVMMLFLPVGLVVYIFVNTAMSVVQQYMMKHDLSFRDVLRGRFKPQTAQNSQ"
     gene            complement(4584..4880)
                     /gene="yidD"
                     /locus_tag="VJC18_02150"
     CDS             complement(4584..4880)
                     /gene="yidD"
                     /locus_tag="VJC18_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HLD44211.1"
                     /translation="MKIFRVFNVVAVFFIRGYQICVSPILPNVCRFEPSCSAYALECF
                     RRFSFFKALRLTLKRLLRCHPFCAGGYDPVPERVGNTKGRGTRGRWTKQLSNLQ"
     gene            complement(4867..5169)
                     /locus_tag="VJC18_02155"
     CDS             complement(4867..5169)
                     /locus_tag="VJC18_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HLD44212.1"
                     /translation="MKKRDILLVNRSRHCLRTRHFIIKVIPNQLAWGRIAFVFPKRFG
                     IAVLRNRFKRRLRVVVSLPSMVGKDFLCIAKGPLSQLTDVLWKNERNKIRTWCENF"
     gene            complement(5200..5349)
                     /gene="rpmH"
                     /locus_tag="VJC18_02160"
     CDS             complement(5200..5349)
                     /gene="rpmH"
                     /locus_tag="VJC18_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HLD44213.1"
                     /translation="MKRTYQPSKLKRIRKCGFRARMKTVGGRAVLSSRRRKGRATLTA
                     SNYKK"
     gene            5859..>6997
                     /gene="dnaA"
                     /locus_tag="VJC18_02165"
     CDS             5859..>6997
                     /gene="dnaA"
                     /locus_tag="VJC18_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HLD44214.1"
                     /translation="MSVNNELLSKVQDSLRTKISDFNYNNWFRNIEWAYEEPSKVVMN
                     VPTKFIRDWLTDNYLELIKFEFFRLTEKEHTITFRVSKQINRTLDLFATQGQTQAQAQ
                     QEPIAKAILPAKKPASPQIPQDLVKYLGFNPRYQFDNYVVGNSNQLVHAACRAVASQP
                     ARSYNPLFIYGGVGLGKTHLLNSIGMQVLKQYPSWRIIYVTGERFTNEVISAIRYGKT
                     YELRQKYRENCDMLLIDDIQFIAGKERTMEEFFHTFNALYEARKQIILTSDKLPRDIP
                     NLEERLKSRFGWGLMADIQRPDFETRIAILRKKAEEDNVSLPNDVCEYIATHVVDNVR
                     DLEGSLIRVSAFASLAKLPITLDLAREVLRKIIIEAKPIMCVENI"
BASE COUNT         1783 a         1800 c         1644 g         1770 t
ORIGIN      
        1 ggcatctgca tggccattgg tgccagctac gcgggagatc ttgccttcac caccacatcg
       61 ggtcccggac tctcgctcaa gtcagaagcc ctggggctcg ctatcatcgc cgaaatccct
      121 ctggttgtgg tcaacgtgca acgcggtggc ccgtcaacag gactccccac aaaaacagag
      181 cagtccgatc tttttcaggc cctgtacgga cggcacggtg aatcccccct gcctgtgttg
      241 gcggccagca caccggcgaa ctgttttgat tacacggtta cggctgccaa gatcgccgtg
      301 gaatacatga cccccgtgat tttgctcacc gatgggtatc tgggcaatgg ctcggaaccc
      361 tggaaactac caaacctcga atccctgccg atcatcaagc cacgggaacc cgcctcccag
      421 cagggagaat ttctttccta tcgccgtgat cccaaaactc tggcgcggga atggtgtgag
      481 cctggcaccc cggggatggc acaccgcatt ggcgggctcg aaaaagaaga catcaccgga
      541 aacgtcagtt atgtcccgca aaaccacgaa ttcatggtac gtgcgaggga cgaaaaaatc
      601 ctgcgcgtcg tcaacgacat cccggaacag cccgtcattg gtgatcagtc gggtgatctg
      661 ctggtggtga gttggggggg gacgtatggg gccgtgctga cagctgtctc tgagatgcgg
      721 actgagggtc atcggatcgg aatggcgcac ttccatcaca tttaccctct gcccaaaaac
      781 accaaaaaca tttttgacca gttcaagcaa atcgttgttt gtgagttgaa tctgggacag
      841 ttccatcaac acctcagcac caactacccc accaagaaac tgctcaaatt caacaaggtc
      901 cagggtcagc ccttcatgat tgccgaactc aaacagtgtt ttgcccatct gctttcagga
      961 gggaattcat gaacacacca caggcctcag agaaaacagt gacaccactc acaaaaaaag
     1021 atttcatgag cgaccaggaa gtccgctggt gtccgggttg cggcgactac gccgtgctag
     1081 cctgcgttca aaaggtgctg gcagaactgg gtgccaacaa agacaacgtg gtttttgttt
     1141 cgggcatcgg ttgttcttcg cgattcccct attacatgaa cacgtatggt ttccacacca
     1201 tccatggccg ggccacggcg gtcgccacgg gagtgaaggt ggccaacccc aaactcgatg
     1261 tttgggtcat ttcgggagac ggggacgcgc tctcgattgg cggaaaccat tttattcacg
     1321 cgcttcgcag gaatatcgga ttcaaactgc tcgtcttgaa caacgaaatt tacggtctga
     1381 ccaagggaca atattccccc acaaccaaac agggttcggt gacaaaatcc tcgccgtacg
     1441 gagcggtgga ccgcaccttt tctcccgctg ccctgggact tggcgccaac gccacatttt
     1501 acgcacgtgc ggtggatacc aacccaaaat tattgactga ggtcattctg gcggcggctc
     1561 agcaccgggg aacttccttt gtggaaattc tgcaaaactg tgtgatcttc aacgacaagt
     1621 gtcatcacgc catcacctcc aaagaaacgc gggccgacaa tcaactgctc ctgcaacacg
     1681 gcaaacccat gctgtttggg gccaacaaca ataaaggcat tattttaaga gggacggaac
     1741 tcatcgtggg cacgctggga gaacggggga tcaccgaaaa ggacattctc atccacgacg
     1801 cgcatgccga gagccccaat ctggcgttca tgttgagtca actcaatctg ccacacgcac
     1861 ccgtcccctt gggaattatc cgccagatct ctgctcccac ttatgaggat tcggtgtgtg
     1921 aacaaattgc aagggtcaag gcatcaaagg gagtcggcga tttcgacaaa ctcctccatt
     1981 ctggagacac ttgggaagtg gggtaacggg ccattcattt cacaacgtac atgatctcgt
     2041 ctggtctgat gtaccccaag tccgtacgca cgcgctgttc gatgtaacgc gattttttga
     2101 gctcttccat ttcctgcaga aaaaacagat tctcccgcag caaaaaatga ctttgtttga
     2161 tgatcccgtc gcgttttgct ttgagggcat gcagttcggt gagcccacca tctccccaga
     2221 cggtgaggga aaaaaacgcc acaaacacac agacagccgc cacgtagagg atgttgttga
     2281 aggtaaagtt aatgagatgg gaattcttga ttcgcttctt cttcggtgtc atgcgcggaa
     2341 agggtaacat atcatcaaga aaaaatctat tcgccattct cggcgggttt ttttcgctgt
     2401 tttttcttct ctccctgaaa ttttttactg ctgagcattt tgtctttggc ccgtctgctg
     2461 cgacgtcttt tttgtgccct gatccggtgc aggcgttttt ctttttcgct ttttttgccc
     2521 aactgaatgg cctcgagcct ttcacacaaa atccgtctgg cgaaaaaacg gttttgttcg
     2581 cgggaacgcg tgtgctgaca gcgaatcacg attcccgaag gggtatgcgt caattgcaca
     2641 cagctgttgg tcttgttgat tttttgtccg ccagcgccac tgcccctgat gaactcttct
     2701 cgcaaaacct ccttggtgac acccaaagcc tcaaggcgtc tggccacatc atcgaatttg
     2761 ctttgcgaga tcggattcat acatcactga ctattttgag cggtttgcgg tttgaatcgt
     2821 ccgcgaagca cgtcccgaaa ggacagatcg tgtttcatca tgtattgttg caccacactc
     2881 atggccgtgt tgacaaagat atacaccacc agacccacgg gcaaaaacag catcataacg
     2941 gtgaacatga caggcatgaa catcatcatt ttttgttgcg cgggatccac actggccgtc
     3001 ggcgtgagtt tctgttgcag gaacatgaac acacccaaca aaatggggga cacaaaatag
     3061 gggtccggcg cactcaggtc ggcatagatc cccaaaaatg gcgcgtgata aagctcgatg
     3121 gcgttccaca acaccttata cagcgcgatg tacacaggaa actgcaacag catgggcaga
     3181 caccccccca tcgggttaac cttgtgcgtg cgaaaaagct gcatggtctc ttcgttctgt
     3241 ttttgtttgt catcgccgta tttctttttg atttcgttga gcttgggttg cagattctgc
     3301 atggccttca tggattgcat ggacttttta ttgaaggggt gtagcaacat tttaacaata
     3361 aacgtaagcg tgatgatcgt gagcccccag ctggggatga atcggtgtaa aaaactcatc
     3421 aacttcagta tgagcactgc aatgatgcta aaccacccat aatccacggc gcgtttaagt
     3481 tcggaactca cacgttccag atacgataat tgcttgggac caacataggc caggaaatgc
     3541 ccacccttct tctgctgggg ggccacaatg agttcattag cgtaaagttg attgacgatg
     3601 ttgtcttttt ctctgccaaa ctgggccttg acagggcctt ggacatcttg gggaagcagg
     3661 gcaaatagaa aataacgatc cgcgatcccg ctccaataga tgtttcctgc aacctcgcgt
     3721 gtgccggcaa atttttgcca gttttgatct tcagtgagtg tcttgtcagc caggtgatag
     3781 agtccgtaca gatcagggcg gtcaaatttt aaaaaagata acaggcctcc cgtttttttg
     3841 gggattggtt tttgcgtgat tgaattttgc agataagggg tgagcaccag atcgctttgc
     3901 gataaattgg ccacctcata ggcaacagtc acggtgtaat tattctgggt cccatcgaaa
     3961 acaaatgttt tctgaatacg cactgaatcg ttttgccatt ccaacacgat ggtgcgaaca
     4021 cccgctggca actcctgatc actcaccacg cggtacacct tccctgctgt caacggcgaa
     4081 taccctttga aacccagaaa aagggccgag gaagttcctt cttccggaag aaggtttttg
     4141 ggaggagagt ccttcttggc tttgagtttg aattttttga gctgataatc ggtgatcaca
     4201 gcaccccgtg tggacagggt gatatgcgcc aggggcgttt cgacagaaat ggtcttttct
     4261 tctgcctgaa tctctccggt ggagggggac tctccttgag gaatctcaag cgactgaggc
     4321 tgctgactct ggataatttg agccggttca acagggaccg ccgtttgctg tgtggtctgt
     4381 gttggctgtt gtggtggttt gggggcaaaa aacatctgat aacccacaaa cactgctgtg
     4441 ctcaaaacta ccgccgaaag taatctaaat tcttgtctca taatattatt tcgtggatcg
     4501 tggatcgtgg atcgtggatc gtggatcgtg gatcgtggat cgtggatcgt catcaactgt
     4561 agcaattact gcaattgttg caatcattgc aagttgctca attgtttcgt ccatcgtcct
     4621 ctcgtccctc gtcctttcgt gtttcccacc ctctccggca ccggatcgta cccaccagcg
     4681 caaaaagggt ggcacctcag tagtcgtttg agggttaaac gtaaagcctt aaaaaaagag
     4741 aaccgcctga aacattcaag agcatacgca gaacagcttg gctcaaaacg acacacattc
     4801 ggcaggatcg gcgacacgca aatttggtag ccacgtataa aaaacacggc cacaacattg
     4861 aagaccctaa aaattttcac accaagtcct tattttgttc ctctcatttt tccacaaaac
     4921 gtctgttaat tgcgacagag gccctttggc gatacacaag aaatccttgc ccaccattga
     4981 gggaagcgaa acaacaaccc gcagtcgtct tttgaaacga ttacgcaata cggcaatgcc
     5041 aaatcttttt ggaaaaacaa aagcgatgcg cccccacgca agctggttgg gtatgacctt
     5101 gatgatgaaa tgacgcgtcc tcagacaatg acgtgaccgg ttgacgagaa gaatgtcacg
     5161 ctttttcatg gatgttttca tgcagacaaa acaacatcat tattttttat aattggatgc
     5221 cgtcagcgta gcacgacctt ttctgcgtcg gcttgataac acagccctgc cgccaacagt
     5281 tttcatgcgt gcccgaaaac cacatttacg aattctcttg agtttgcttg gttgatatgt
     5341 gcgtttcatt ttttgcctct aaattatatt tatttttcag tacgataaaa gagcgtcccc
     5401 ttttagtgta tttttagaaa aagtcaatgc tggtcatgcg gaaaaaatta catagtcttc
     5461 ttcatgggtt gaggaattga aaaaaaaaaa tagcaaaatt cctttacagt gcatttcgac
     5521 agcactaata ctctacgggt tgcctctaaa gacatcttgt gcacaacttg ccagttcttt
     5581 gaatttacaa agctttttaa taacctgtaa atggtgggcc cagtttttcc acaaggcgtt
     5641 ttccacaagc aattgtcaaa ccctgtgtat tcttagccca ggtcaatggt aaagagtttt
     5701 tctttggttt tttaagaagt tttgtcattt caaagacact acaatttgtt aactcattgt
     5761 aatttttgtc tttcctcgca acaaggggtt tattgcctta aattagcggc aggggttcgc
     5821 ttttagggca gttcatttta ctctgtttag ggggacttgt gtctgttaac aacgaattat
     5881 tatcaaaagt gcaggattcg ctcaggacaa aaatcagcga ttttaactac aacaattggt
     5941 ttcggaacat cgaatgggcc tatgaagaac cttcaaaggt cgtcatgaac gtgcccacaa
     6001 aattcatccg cgactggctc accgacaact atttggaact catcaagttt gaatttttta
     6061 gattgactga gaaggaacac accataacct tcagagtgtc caaacaaatc aacaggactt
     6121 tagatttatt cgcaacgcag ggacaaacac aggcccaggc acaacaagaa ccaatcgcca
     6181 aggcaatcct tcctgcaaaa aaaccggcgt ccccccagat cccacaagat ttggttaaat
     6241 atcttggttt taatccccgt tatcagttcg acaactacgt ggtgggaaat tccaatcaac
     6301 tggtacacgc cgcttgtcgt gctgtcgcct cgcaacccgc acgctcctat aatcccctgt
     6361 tcatctacgg cggtgtgggg cttggcaaaa cccatctgct caactccatc ggcatgcagg
     6421 tgctcaagca ataccccagc tggcgcatca tttatgtgac tggtgagcgc ttcaccaacg
     6481 aggtgatcag cgcgatccgt tatggcaaga cctacgagct ccgccaaaaa tatcgcgaga
     6541 actgcgatat gctgctcatc gacgacattc aattcattgc aggcaaagaa cgcaccatgg
     6601 aagaattttt tcacaccttc aacgccctgt acgaagcccg caagcagatc atcctcacca
     6661 gcgacaaact cccccgagac atccccaacc tcgaagaacg cctcaaatcc aggtttggct
     6721 ggggtttgat ggccgacatt cagcggcccg atttcgaaac gcgcattgcc atcttgcgca
     6781 aaaaagccga ggaagacaat gtctcgctcc ccaacgatgt ctgtgaatac atcgccacgc
     6841 acgttgtcga caacgtacgg gacctcgaag gttctctcat ccgcgtttcg gcctttgcct
     6901 cgctggccaa attgcccatc acgctcgatc tcgctcgtga ggtcttgcgc aaaatcatca
     6961 tcgaagccaa acccatcatg tgtgtagaga acatcca
//
LOCUS       DATDUN010000159         2373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_297999, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000159 DATDUN010000000
VERSION     DATDUN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297999"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VJC18_02170"
     CDS             <1..507
                     /locus_tag="VJC18_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLD44215.1"
                     /translation="VTIQKGPDYFVEAAHEVLKKVKDVRFVLAGSGDMLTRLIERVAH
                     YRMQKNFHFTGFLRGREVEEMFALSDLYVMPSVSEPFGVTPFEAMLYHVPVIISKQSG
                     IAEILKSAVKIDFWDVQKLADAIVELLTDSKLVDQLVAQGAQELDHVSWDTAAQKIKW
                     HYREVIKS"
     gene            504..1706
                     /locus_tag="VJC18_02175"
     CDS             504..1706
                     /locus_tag="VJC18_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HLD44216.1"
                     /translation="MINVCFYFQVHQPFRLRKDYSFFKIGRDHHYDDDATNRAVMRKV
                     AEKCYLPTNALMLELIKKHQGNFRISYSLSGVALEQFELYARDVLESFQELVATGCVE
                     LIDETYYHSLAFLYSHTEFRAQVEAHTKKIKELFHGYQPTAFRNTELIYNNDLAKVVE
                     DMGYQVILTEGANQILGWRSPNFVYQPEPCYRMKLLLKNYKLSDDVAFRFSDKGWSEH
                     PLDVRKYAHWIHEVAGNGEVVGLFMDYETFGEHQWADTGIFEFMKVLPDAVFEHPDFC
                     FKTPSEVALNCQPVAKVDVPYFISWADLERDTTAWLGNSLQDSSAEYLYSLEDAVLRS
                     GDIGLIHQWRKLQTSDHFYYMCTKWFSDGDVHKYFNPYNTPHDAFITFNNALNDLREQ
                     LRARGVSD"
     gene            1721..>2373
                     /locus_tag="VJC18_02180"
     CDS             1721..>2373
                     /locus_tag="VJC18_02180"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HLD44217.1"
                     /translation="MTMNRKITTFTVLPDTPPKLKHLRELAYNLYFSWNHEVLELFQK
                     IDPEAWKFAHDNPVRMLCLVSQEKLEVAAKDKNFQAEMGAIHDRFKRYMEGTTWFDRE
                     FGERATPTIAYFSAEYGIHECLPIYSGGLGALSGDHIKSASDLGIPLVAVGLLYSQGY
                     FRQYLNAGGMQQESYPLNDWYSKPVHVVKDPEGKPLTGQVEMLGEPVYYHVWRVDVGR
                     "
BASE COUNT          558 a          578 c          655 g          582 t
ORIGIN      
        1 gtgactatcc agaagggccc cgattatttc gtggaagcgg ctcacgaggt gctcaaaaaa
       61 gtgaaggatg tgcgtttcgt gctggcgggt tccggcgaca tgctcacgcg tttgatcgag
      121 agggtggctc attaccgcat gcagaaaaat tttcacttca ccggtttttt gcgcggtcgt
      181 gaggtcgagg agatgtttgc cctcagcgac ctctacgtga tgccttccgt gtccgaaccg
      241 ttcggtgtca caccgttcga ggccatgctg tatcacgtgc cggtcatcat ttccaagcaa
      301 tcgggtatcg ccgagatcct caagagtgct gtcaaaattg atttttggga tgttcagaaa
      361 ctggctgatg ccattgtgga gctgttgact gattccaaac tggtcgatca gcttgtggca
      421 cagggcgcgc aggaactcga ccatgtgagc tgggacaccg cggcgcagaa gatcaagtgg
      481 cattaccgcg aggtgatcaa atcatgatca atgtgtgttt ttattttcag gtgcatcagc
      541 catttcgctt gaggaaggat tacagctttt tcaagatagg gagagaccat cattacgacg
      601 atgatgccac caatcgggcg gtcatgcgca aagtggccga aaaatgttat ctgcccacca
      661 acgccctcat gctggaactc atcaaaaagc atcagggaaa cttcaggatc agttactccc
      721 tcagcggggt ggccctggag cagttcgaac tttatgccag agacgtgctg gagtcgtttc
      781 aagaactggt ggccacaggt tgcgtggagc tcatcgatga gacctattat cactctctgg
      841 cgtttctgta ttcgcatacc gaatttcggg cacaggttga ggcgcacacc aaaaaaatca
      901 aggagctttt tcacgggtat caacccacag cgtttcgtaa cacagagttg atctacaaca
      961 acgacttggc caaggtcgtg gaggacatgg ggtatcaagt cattctgacg gaaggggcca
     1021 atcagattct gggttggaga agcccgaatt tcgtgtatca gccggagccg tgctaccgca
     1081 tgaagctcct gctcaaaaac tacaagctca gtgacgatgt ggcgttccga ttttccgaca
     1141 agggctggtc ggaacatccc ctggacgttc gtaagtatgc ccactggatt cacgaggtgg
     1201 cgggcaatgg cgaggtggtt gggttgttta tggattacga aacctttggt gagcaccagt
     1261 gggccgacac ggggattttt gagttcatga aggtcttgcc cgacgcggtt tttgagcacc
     1321 cggatttttg tttcaaaacg ccttcagagg tggctctcaa ctgtcaacct gtggccaagg
     1381 tggatgtgcc ttatttcatc tcctgggccg acctggaacg cgacacgaca gcgtggttgg
     1441 gcaattcgtt gcaggattca tcggctgagt acctttattc cctggaggac gctgttctgc
     1501 gatccggtga catcggactg attcaccagt ggcgcaagct tcagacctcg gatcattttt
     1561 attacatgtg caccaagtgg ttcagcgatg gagacgtgca caaatatttt aacccttaca
     1621 acacaccaca cgacgctttc atcacgttca ataacgccct gaacgatttg cgtgaacagt
     1681 tgcgagcccg cggggtgtct gattaacaac ggatactggc atgacgatga acagaaaaat
     1741 cacaaccttc acagtgttgc ccgatacacc tcccaaactc aagcatttgc gggaattggc
     1801 ttacaatctt tatttttcct ggaatcacga agtactggag ctgtttcaaa agatcgaccc
     1861 cgaggcctgg aagttcgctc acgacaatcc tgtgaggatg ttgtgtctgg tcagtcagga
     1921 aaaacttgag gtcgctgcca aagacaaaaa ttttcaggct gagatggggg ccatccatga
     1981 tcgtttcaaa agatacatgg agggcaccac ctggttcgat cgagaatttg gagagcgcgc
     2041 tacccccacg atcgcgtact tttcggccga gtacgggatt cacgaatgtc tgcccatcta
     2101 ttccggcggt ctgggtgcct tgtcgggcga tcacatcaaa tcagcgagcg atttgggtat
     2161 ccctttggtg gctgtgggac tcttgtattc ccagggatat ttccggcagt acctcaacgc
     2221 gggaggcatg caacaggaga gttacccctt aaacgactgg tacagcaaac ctgtgcacgt
     2281 ggtcaaagac ccggaaggca aacctctcac gggtcaggtg gagatgcttg gtgagcctgt
     2341 ctattaccat gtctggcgcg tggacgtggg ccg
//
LOCUS       DATDUN010000160         1330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_463942, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000160 DATDUN010000000
VERSION     DATDUN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463942"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VJC18_02185"
     CDS             <1..546
                     /locus_tag="VJC18_02185"
                     /inference="COORDINATES: protein motif:HMM:NF045331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44218.1"
                     /translation="PGTILKRAMSGEFDMMGEIQKVLGELKAGYTIKPLGDWESGTYR
                     DRVNLAKKLAIYACGVAVQKYMADIKDQQYALELMANLVIEVFAMDCAYKRTQQILAK
                     DATQKIPSYLAGIYIAEAYDKMLMIARRLVGEIADGDENLVGKYQKALTRFDLFAPIN
                     TNKLRELVATHMVERGGYALK"
     gene            complement(595..1197)
                     /locus_tag="VJC18_02190"
     CDS             complement(595..1197)
                     /locus_tag="VJC18_02190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HLD44219.1"
                     /translation="MQLILASKSPRRHQLFKQLLIDINAATKNFNIIIPDIDESCLAS
                     ESPLTCAKRLALEKARSIITESDALILTADTIVVLDKQIYGKPLDEEDAIRMLGDLCG
                     RTHEVITGYCLLITEDGKEKQIINDHDSSLVTFRTYSLDEIKKFVATGIPLDKAGAYA
                     VQEDMFGFIQKIEGSYTNVMGLPVEKIAKDMSELLRRPTI"
BASE COUNT          330 a          282 c          373 g          345 t
ORIGIN      
        1 ccgggcacga tcctcaaacg cgccatgagt ggtgagttcg acatgatggg tgaaatccag
       61 aaggtgttgg gtgaactcaa ggcaggctac acaatcaaac cactcggtga ttgggagagt
      121 ggcacgtacc gtgatcgcgt gaatctggcc aagaagctgg cgatctatgc ctgcggggtg
      181 gcggtgcaga aatacatggc cgacatcaag gaccaacagt acgcgttgga gctcatggcg
      241 aatctcgtca tcgaagtgtt cgcgatggac tgcgcgtaca aacgcacgca acagatcttg
      301 gccaaggatg ctacgcaaaa gattccatcg tatctggcgg gaatttacat cgcagaggcg
      361 tacgacaaga tgctcatgat tgcccgcaga cttgtgggtg agattgccga tggtgacgaa
      421 aacctggtgg gcaaatatca aaaagccctc acgcgttttg atctcttcgc gcccatcaac
      481 acgaacaagc tcagggagct ggtcgcgacg catatggtgg agcggggcgg gtacgcgttg
      541 aaataaaagg tcatgagtga tgagtcatgt gtgatgagtc atgaagatgt ggtgctatat
      601 tgtagggcgg cgcagtagct ccgacatatc tttcgcaatc ttttccactg gcaggcccat
      661 gacgttcgtg tatgatcctt caatcttctg aatgaacccg aacatgtctt cttgcacggc
      721 ataggcaccg gccttgtcca aggggattcc cgtcgcgacg aactttttta tttcatccaa
      781 gctgtaggta cggaatgtga caagactgga atcgtgatcg ttgatgattt gtttttcttt
      841 gccgtcctct gtgatcaaaa gacaataccc cgtaatgacc tcgtgagttc tgccacacag
      901 gtcaccgagc attctgatgg catcttcttc atctaggggc ttcccgtaaa tttgtttatc
      961 aagcaccacg attgtgtcgg cggtcaagat gagcgcgtca ctttctgtga ttattgatcg
     1021 tgccttttcg agggccagtc ttttggcgca ggtgagtggc gattcgcttg caagacagga
     1081 ttcatcgata tcggggatga tgatgttgaa attttttgtt gcggcattga tgtcgatcaa
     1141 caattgtttg aacagttgat ggcggcgggg ggatttgctg gctaaaatga gttgcatgaa
     1201 ataaaaaaac taactaaatc tgcgtatata tgatgtgaca aagggaaagc aaatgcccaa
     1261 tatgaaaaaa aaacagtgca gtatcctttt gggtgttttg gtcttgtgtc tgaattgttt
     1321 tggcgggggg
//
LOCUS       DATDUN010000161         3917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_257353, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000161 DATDUN010000000
VERSION     DATDUN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257353"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VJC18_02195"
     CDS             complement(<1..1289)
                     /locus_tag="VJC18_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44220.1"
                     /translation="MRHYLVIILVLCSLPALAAGPMINETTTSGEEIISTPPVWDNSA
                     PIEYHLESGSCGPFSNEEMIEKYATDMGVWSGIDSVDLSFLGVTGELDGIDFDNYTDY
                     FYLTSDSDESLLTDGINPIAFDDDGAITVAIFGDGNQYLLLGFAGISYYADGEVLDGQ
                     SVINCLCIVDNPAGPCVIESEAGDVTVEMTEADLDFTIVHEMGHFLNLGHTNVNSDIY
                     MADSYSEGDIPIMFPIASGMQTITPHEDDIMSLTTLYPSGEVSDHYCVVEGHIMDSTG
                     ENYVPCVDVWAVTDDEDLTQTLSYTTASNNYFNTMTTNDFDPTGTCDANCGFFSLHIK
                     PGTAYTLRAYDVYHSFTEASSVGPCTTEQPAYGLTGVELMTITDSDCPSATDEPIEAG
                     EISVEAEVDSSGGDEDGGSGDSDKLDEDEKFNPIGHG"
     gene            complement(1487..2635)
                     /locus_tag="VJC18_02200"
     CDS             complement(1487..2635)
                     /locus_tag="VJC18_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44221.1"
                     /translation="MEYKRSLNLLSLLENKSFFLFGPRSTGKTWLIKQQLQGKAIIIG
                     LLTSDNLIRLTQNPGEIKDIILAHPEKKLVVIDEIQKIPSLLSEVHQLLDTSDLRFLL
                     TGSSARKLKKGNADMLAGRAWNASLFPLTFLEIDEFDLEHYLRWGGLPQVYPSRKPDE
                     ELSAYVETYLREEILAEGLIRKLPPFSRFLKTAALSNGQLINFTELGNDAAVSPSTVR
                     EYFNVLTDTLIGFQLEPWLSSKKRKAIQTAKFYFFDLGVTHALANTKNLERNSNLYGA
                     SFEQFIGLELRAYLSYSRIKENLTFWRSINGQEVDFLIGEKVAIEVKATNRITSSDTK
                     GLRTLAEEGVFKKYYLVSQDETIMKKDFIECLHWKEFLNRLWSGNGLE"
     gene            complement(2948..3493)
                     /locus_tag="VJC18_02205"
     CDS             complement(2948..3493)
                     /locus_tag="VJC18_02205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLD44222.1"
                     /translation="MDNISKLPSVSDRTYTRVNAKLYLHSEHYSPTRGPQHDSKGLFM
                     APFAEEQDDVVGLSIDAFWEKVSESSVENKDLFLELQQILAMLKRAEKPDPMRGTHKS
                     FKKLNVNISGSGIAFPSDVAYHAGQLLRLSLFFPKKPFSYITVIVEVVRSEKAEIGYD
                     IKCRYKEISDDIREEILAFVS"
     gene            complement(3486..>3917)
                     /locus_tag="VJC18_02210"
     CDS             complement(3486..>3917)
                     /locus_tag="VJC18_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44223.1"
                     /translation="SSVGPCASAQLTVCTDSIITACTDDVASTTCTACVKEDPLTTND
                     ASADIAAKIQAECTAGAVVSLGDITTRSVSKPSAAESNGDGDSDDDDNTSSSSSGCSL
                     NPDVKKATGAMGFFCLIILGPLLSFLTKRLTIIHNPEPNNG"
BASE COUNT         1043 a          950 c          877 g         1047 t
ORIGIN      
        1 cacccatgcc caatgggatt aaatttttca tcttcatcca atttgtccga atccccagag
       61 ccaccatctt catcacctcc tgaggaatcc acctcagcct ccaccgatat ctcccccgcc
      121 tcgatcggtt cgtcggtggc agagggacaa tcactgtctg tgatcgtcat gagttctaca
      181 cccgttaatc catacgccgg ttgctctgtc gtgcacggcc ccacgctcga ggcctccgtg
      241 aaagaatgat agacatcgta cgccctcaac gtgtacgcgg tccccggttt gatgtgcagg
      301 gaaaaaaagc cacaattggc atcacacgtg ccggtggggt caaaatcatt cgtggtcatt
      361 gtgttgaaat aattatttga agccgttgtg tacgataatg tctgcgtcag atcttcatca
      421 tcggtcacgg cccacacatc cacgcagggc acatagttct ccccggtact gtccatgatg
      481 tgcccttcaa ccacacagta gtgatccgag acctccccac tgggatacaa cgtggtgagc
      541 gacatgatgt cgtcttcgtg tggtgtgatc gtttgcatgc ccgatgcgat gggaaacatg
      601 atggggatgt cgccctcaga atagctatcc gccatgtaaa tatcggagtt gacattcgtg
      661 tggcccaggt tcaaaaaatg gcccatctcg tgcacaatcg taaaatccag atcggcttcg
      721 gtcatctcaa cagtgacatc tcccgcctca gattctatca cacagggtcc tgcgggattg
      781 tcgacgatac acaaacaatt gatcacggac tgaccatcaa gcacctcacc atctgcgtaa
      841 tacgagatcc ctgcaaaacc cagcaacaag tattggttgc catcgccaaa gatagccact
      901 gtgatggcgc catcgtcatc aaacgcgatg ggattgatcc catcagtcag taaactttca
      961 tcggaatctg acgtcaggta aaaatagtcc gtgtaattgt cgaaatcgat accatccagc
     1021 tctccggtga ctcccaaaaa acttaaatca acactatcga tcccgctcca cacgcccata
     1081 tctgtcgcgt atttttcgat catctcttcg ttcgaaaaag gaccgcagga tccagattcc
     1141 aaatgatact ctataggagc agaattgtcc caaacagggg gagtggatat gatctcttca
     1201 ccagatgtcg tggtctcgtt gatcatcggg cccgcggcca gggcaggaag ggaacaaaga
     1261 acaagaatga tgacaagata atgccgcatg acacacatat cgcctcatat tgtatctcca
     1321 ttcccaacaa accaaaagta aaaaagatga ttttttaatg agttcagaca gatatggtgg
     1381 aaacgaacat gaatgaatgg catcgaatct gttctgggac ggttgtgtga caaaccagtg
     1441 cggttgctgg tcccgcatca tcagagcaag attttgagcg aggtcttcac tcaagaccat
     1501 tcccactcca gagccgattc agaaattctt tccagtgcaa acattctatg aaatcttttt
     1561 tcatgatcgt ttcatcttga gagacgaggt aatacttctt aaacacaccc tcttcggcca
     1621 aggtacgcaa tccctttgtg tcagagcttg tgattctgtt ggtcgctttg acttcaatgg
     1681 ccaccttctc tccaattaaa aaatccacct cttgtccatt gatggatctc caaaaagtca
     1741 ggttttcctt gatgcgtgag taacttaaat atgctcgcaa ctccagacca atgaactgtt
     1801 caaaactggc cccatagaga ttggaatttc tctcaagatt ttttgtgttg gccagggcgt
     1861 gggtcacacc caagtcaaag aaataaaatt ttgcagtttg aatcgccttg cgtttttttg
     1921 aagataacca gggttccagt tgaaaaccaa tcagtgtgtc cgtaaggaca ttgaagtatt
     1981 ccctgacagt cgatggcgaa acagcggcat cgttccccag ttcagtgaaa ttgatcagct
     2041 gtccattgga gagcgcggcc gtcttgagga accggctaaa cggaggcaat tttctgatta
     2101 gcccttctgc caaaatctct tcgcgcaggt aggtttctac gtaggcagac agttcttcgt
     2161 caggttttct gctcgggtac acctggggaa gacctcccca ccgcaaatag tgctccaaat
     2221 cgaactcatc aatttccagg aaagtcagtg gaaaaagact ggcattccag gcacgtccag
     2281 ccaacatatc cgcatttccc ttttttagtt ttctggcact gctcccagtg agcaggaatc
     2341 gcaaatcgct tgtgtcgagc agttgatgca cttcgctgag aagggacggg attttttgaa
     2401 tttcgtcaat gacaaccaat tttttttctg gatgagccaa aatgatgtct tttatttcac
     2461 ccggattttg ggtcagtctg atcaaattat cagaagtcaa aaggccgatg atgatcgctt
     2521 tgccttgcag ttgttgtttg atgagccagg tctttcctgt tgatctgggt ccaaagagaa
     2581 aaaaagactt gttttcgagt aaagagagta aatttaggga ccgtttatat tccatataca
     2641 gcaattgtac tgctactata ataatttaac aacaatatta ttgctgatag tttagtatgc
     2701 ttttagacat gttaaatgga taagcaaatg gttctacaca catgaatatg acccacaact
     2761 cgttttctgt catatttaca attacttaaa attcaatacc gttcctccgt catataaata
     2821 ccgttcctcc gtcatatcaa tcctatgctt gtagaagtgg ctggctgaac atggggatca
     2881 aagagtgatg cccccttgat cttgatttgg tgccagcccg ttggctctgg cggtttgtag
     2941 gtgttgttta cgaaacgaaa gcaaggattt cttcgcggat atcatcactg atctctttat
     3001 accggcattt gatatcgtag ccaatttcgg ccttttcgct acgaacaacc tcgacgatca
     3061 cagtgatgta ggaaaatggt tttttaggaa aaaacaggga aagacgcaaa agttgacccg
     3121 cgtgataagc cacatcggac ggaaacgcga tgccagaacc actgatattg acattgagtt
     3181 ttttaaacga tttatgagtg ccgcgcatgg gatcgggttt ttcggcgcgt ttgagcatgg
     3241 ccagtatttg ttgtaattcc aaaaacagat ctttgttttc aaccgaagac tcactgacct
     3301 tctcccagaa cgcgtcgatc gacaaaccca ccacatcgtc ctgctcctca gcgaaggggg
     3361 ccataaaaag ccctttgcta tcgtgttggg ggccacgtgt aggtgaataa tgctcgctgt
     3421 gcaaatacaa cttggcattc acacgtgtgt aggtcctatc ggaaacagat ggtaattttg
     3481 aaatgttatc cattgttggg ttctgggttg tgaatgatag taagacgttt tgtcaaaaaa
     3541 ctcaagaggg ggcccaaaat aatcagacag aaaaacccca tagcccctgt tgccttcttg
     3601 acatcagggt taagagaaca cccagaactg gacgacgagg tgttgtcatc atcatcagaa
     3661 tcaccatcac cattgctttc ggcggcggat ggtttgctga ctgatcgtgt ggtgatgtca
     3721 cccaaactca ccacagcacc agcagtgcat tcagcttgaa ttttcgcggc aatgtcggca
     3781 ctggcatcgt tcgttgtcag aggatcctcc ttgacacaag ccgtacaagt tgtgctggcc
     3841 acatcatctg tgcaagccgt gatgatcgaa tccgtacaga ctgtcaattg ggcggaagcg
     3901 cacggtccca cgctgga
//
LOCUS       DATDUN010000162         1141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_589547, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000162 DATDUN010000000
VERSION     DATDUN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589547"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VJC18_02215"
     CDS             complement(<1..248)
                     /locus_tag="VJC18_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HLD44224.1"
                     /translation="MRTRPELENIENNSLSPYSAKSAGTLGRQHTESEHPYRTAFQRD
                     RDRIIHSSAFRRLEYKTQVFINHEGDYYRTRLTHTMEVS"
     gene            529..>1141
                     /locus_tag="VJC18_02220"
     CDS             529..>1141
                     /locus_tag="VJC18_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLD44225.1"
                     /translation="MHPLIITCAITGAETRKSDQPNLPITPKEQAKAAKEAVKAGASV
                     IHLHVREDDGKPSQRLERFEEAISEIRTLVPEVIIQISTGGAVGVPIEKRAKPLELKP
                     EMASLNIGTMNFGDDVFYNHPLDVRALAKKMDELGVIPELEIYDAGMMETAITYLEKG
                     IIKSPAHLQFVLGVRGGMNGDIKNLVALKNLMDLHMSKETHWGI"
BASE COUNT          286 a          247 c          325 g          283 t
ORIGIN      
        1 gaaacctcca tggtgtgtgt gaggcgtgtg cggtaatagt cgccttcgtg attgatgaag
       61 acctgtgttt tgtattcgag tcttctgaag gcgctggagt ggatgatgcg gtcacggtca
      121 cgttgaaagg ctgtgcggta ggggtgttcg ctttcggtgt gctgtcgccc gagagtcccg
      181 gcacttttgg cactgtaagg tgacagagaa ttgttttcga tgttttctag ttctggtcgt
      241 gtgcgcataa gcgtggatcg tggatcgtgg atcgtggatc gaaaacagct caaagctcaa
      301 agctcaaagg gagccgaatg tcgaataacc aacaaggaac aaggaatttt gaagtaaaag
      361 tctctcattt cgacattcga aattccttgt tcgaaattcg atattcaaaa gttcttttgc
      421 ccttatcata ttaatcaggc ctgcggtcaa gcttgcggtg atgttcgctg tggcaatgtg
      481 gttgatattt tgggttggta tggtaaggat cgtccaaagg agtctgccat gcaccctctc
      541 atcatcacct gcgccatcac gggcgccgaa accagaaagt ccgatcaacc caacctgccc
      601 atcacaccca aggagcaggc caaggccgcc aaagaggctg tcaaggctgg cgccagtgtg
      661 attcatttac atgtgcgtga ggatgatggc aagccaagcc agagattaga gcgattcgaa
      721 gaggcgattt ctgagatccg gacactggtg ccagaggtca tcattcaaat atccactggt
      781 ggcgctgtgg gagtccccat cgaaaaacgc gccaaaccct tggagttaaa acctgagatg
      841 gcttcgctca atatcgggac catgaacttt ggtgatgatg tattttacaa tcacccgctc
      901 gatgtgaggg cacttgccaa aaaaatggat gagttgggag tgatccctga gcttgagatt
      961 tacgacgcgg ggatgatgga aacggccatc acttatctcg aaaaggggat catcaagagc
     1021 ccggcgcatt tgcagtttgt gctcggggtt cggggcggga tgaatggtga cataaaaaat
     1081 ctggtggccc tgaaaaatct catggacctg cacatgtcca aagaaacaca ctgggggatt
     1141 g
//
LOCUS       DATDUN010000163         1105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_423352, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000163 DATDUN010000000
VERSION     DATDUN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423352"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VJC18_02225"
     CDS             complement(<1..693)
                     /locus_tag="VJC18_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HLD44226.1"
                     /translation="MNQTILENKVTGFLSRYGQILLVCVVLIVMTLMIWPIEPAILDA
                     LLTINIIGALIVLMLAITISHPMKLSSFPSLLLICTLFRLGLNVSSTRLILSRGEAGE
                     IINTFGYFVTEGNFVVGVIIFFVLLVIQFIVVAKGAERVAEVAARFTLDALPGKQMSI
                     DADLRAGLITSEQAADRRNDLIKESKLYGSMDGAMKFVKGDTIAGFVITFINIMGGLI
                     IGVFQRGLEVGVA"
     gene            complement(855..>1105)
                     /locus_tag="VJC18_02230"
     CDS             complement(855..>1105)
                     /locus_tag="VJC18_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44227.1"
                     /translation="CVTFINNNIPSESVSREFILLAGQQASLQTDAFYREYGRVVTSE
                     VPAQISVIQSYHVQPVPGQEEEEYQVPVFGEELLAMAK"
BASE COUNT          297 a          300 c          254 g          254 t
ORIGIN      
        1 cgccactccc acttccagtc cacgttgaaa caccccgatg atgagcccgc ccataatgtt
       61 gatgaaggtg atcacgaacc cggcaatggt gtcgcctttg acaaacttca tcgcgccatc
      121 catcgaacca tagagcttgg attccttgat caggtcgttg cgcctatcgg ccgcctgctc
      181 gctggtgatg agccccgccc gcagatcggc gtcaatgctc atttgtttac cgggcagggc
      241 atcgagtgtg aatcgggcgg ccacctcggc cacgcgttcc gcccccttgg ccaccacaat
      301 aaactgaatc acaagcaaca caaaaaagat gatcacgccc accacaaaat tcccttcggt
      361 cacaaaatag ccaaaagtgt tgatgatctc accagcctca ccgcgcgaga ggatcaaacg
      421 ggtggaactc acgttaagtc ccaaccggaa caaggtacag atgagcaaaa gagagggaaa
      481 actggaaagc ttcatgggat gactgatggt gatggccagc atcagcacaa tgagcgcccc
      541 tatgatgttt atggtgagca gggcgtccag aatggctggc tcaatcggcc agatcatgag
      601 ggtcatgaca atcaagacca cacagaccaa caggatttgg ccataccgcg ataaaaagcc
      661 cgtcacttta ttttccagga ttgtttggtt catgctcctt catagagcaa ggtctgtgcc
      721 aaaatcggaa atattatttt gattgcgatt tcaatgggtt gaaaattttc ccatctcatt
      781 tcccgtccca ctgtcttggt tttggaacag tccctgagac accctagaca gacatccatg
      841 atgggcgaaa acaattattt cgccatcgcc agcagttcct ctccaaacac cgggacttga
      901 tattcttcct cttcctgtcc aggcactggt tggacatgat aagactgaat gacactgatc
      961 tgtgcaggca cttcggaagt aaccactctg ccatattcac gataaaaggc atctgtttgc
     1021 agagacgcct gctgtcctgc cagcagaatg aattcccgtg aaacactttc ggaaggaata
     1081 ttgttattga tgaacgtcac acaat
//
LOCUS       DATDUN010000164         1204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_48905, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000164 DATDUN010000000
VERSION     DATDUN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48905"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VJC18_02235"
     CDS             complement(<1..482)
                     /locus_tag="VJC18_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44228.1"
                     /translation="MKKMISILLMFCVFFAPLSSALAVDPTLPDSTIQTINNNVEVRL
                     YAYKKTKHYTAVEIGFTNNTNHYVTFSPKEIYLDDEVKYSQSLLSMDQIRIIEQEKPG
                     MAIFPTAIAVGLGIAALGTSRSHNDVAFGLAMGALGAGGAALLTKGLEDRAKQNKFIA
                     "
     gene            702..>1204
                     /locus_tag="VJC18_02240"
     CDS             702..>1204
                     /locus_tag="VJC18_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44229.1"
                     /translation="MSQDGTNLKIDRLVAFLICGEEFLDVLEQIYLLCRYLSGPNNIH
                     VILPPHLKNAGIGFYEKIKSTKATVHVLHATNVASFASRREIKKIVRSHQIRLLHTYD
                     VVAAQAVAKINLRKIACEHIMTVTDHNPYLLASGFKGRGLRKVLAQASQTIFTSHHAQ
                     EKLVARV"
BASE COUNT          293 a          292 c          300 g          319 t
ORIGIN      
        1 aacgcaatga atttgttttg tttggcgcga tcctcaagtc ctttggtgag caaggccgcc
       61 ccaccggcac ccaaggcccc catggcgagg ccaaaggcca catcgttgtg cgaacgggat
      121 gtgcccagcg cggcaatgcc caaaccaacg gcgatggctg ttggaaaaat ggccatgcct
      181 ggtttttcct gctcgatgat tctgatttga tccatagaca gcaaggattg tgagtatttt
      241 acctcgtcgt ccagataaat ttccttgggg ctgaacgtca catagtgatt ggtgttgtta
      301 gtaaaaccaa tttcaacggc ggtgtagtgt ttggtttttt tgtatgcgta caatcggacc
      361 tcgacgttgt tgttgatggt ctgtatggtg gagtcgggca aggtaggatc aacggccagc
      421 gctgatgaca agggtgcaaa gaaaacacag aacatcaata gaatggaaat catttttttc
      481 atactcacct cgtgccgggg atcttatcag aaaagtttat ctgatttcaa tctttcatct
      541 tacacaaggc ctacggtcag tggtcgacgg tcaacagctt acggccgacg gccggaaata
      601 ggtttggcag tcgaccgttg gctgtaagcc gtgagccttt cgctttgagc ttcttccttg
      661 tatccacttg aataccagtc cctttcagcg tatggggccc catgagtcag gatggcacaa
      721 atctcaaaat tgatcgcctg gtggcttttt tgatttgcgg tgaagaattt ttggatgtgc
      781 ttgagcagat ttatctgctg tgtcgttacc tatccggccc aaacaatatt catgttattt
      841 tgccacctca tctcaaaaac gcggggattg gcttttacga aaaaatcaag tccacaaagg
      901 ccacggtgca tgtcctgcat gccacaaatg tggccagttt tgccagtcgt cgcgagataa
      961 aaaaaatagt acggtcccat cagatcaggc ttttgcacac gtacgatgtt gttgccgccc
     1021 aagctgtggc caaaatcaat ctgcgcaaga tcgcctgcga acacatcatg actgtgaccg
     1081 accacaatcc ctatttactc gcgtctggct tcaagggaag gggtctgcgc aaagtcctgg
     1141 cgcaggcctc ccaaaccatc ttcacgtcac atcacgcgca ggaaaaattg gtcgctcgtg
     1201 tcaa
//
LOCUS       DATDUN010000165         2135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_505965, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000165 DATDUN010000000
VERSION     DATDUN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505965"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..1275
                     /locus_tag="VJC18_02245"
     CDS             496..1275
                     /locus_tag="VJC18_02245"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLD44230.1"
                     /translation="MFNHLQQREIFHLMFLRHFCPKIKTNTLSLKGGVNLRFFFNSHR
                     YSEDLDLDIKDIETFQLKDIVMGILESRSLALTLRPFHINKIIPPDINVAKQTETTQR
                     FKVHLITASSENLFTKIEFSKRPMRDDIVVERVRDDVVFPYKIQTLSIPHYTAQAAFL
                     QKIIALAERGKTQARDIYDLAWLKAFVDSQALNCRHRLGTEICAKAKGNIFKISFAIF
                     RDTVQNYLNEEERLLYQHPDSWEELQLRVCQIIEEIVENRL"
     gene            1272..1877
                     /locus_tag="VJC18_02250"
     CDS             1272..1877
                     /locus_tag="VJC18_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44231.1"
                     /translation="MKANFAQMILVHEQTVFTTREAAERCHTSLASASRGLALLTKRG
                     LLTHVMKSVWAKTSDKTFSAHQVIPHLNHKHQFYLSFTSALSLHGVLSQLPQRVTVAS
                     TAHSGMVKTPVGVFEIHQLAPELFQGFDWDSAHQYLLATPEKALVDCLYLSFRKKKQF
                     GYFPEIDMSLLNQKNIMKWINLIRDQRTKSLVKAKLKELLV"
BASE COUNT          609 a          492 c          481 g          553 t
ORIGIN      
        1 ggcacctttg gcggaaagtg ctactaccag tgcatcgatt gacaaactgt tacttgagca
       61 ttcagtgtat gagccaaaca tgacgtctgc aagatcggcc ggatttttgg ccgcacatgg
      121 agagagcaat tttatcgtca cagaagttta tgagtgttca cccaacaagt aaaaggtaaa
      181 aggtgccagt ccccgtaaag cttcgccacg gggcaagcaa caatcgaaca aaaaagtgcc
      241 acccactttt tccaaaccca ttgggtcggt tgcaaccgac cactacgtct tggaaaccaa
      301 atgcgatgta atgacggagc aatgatgttg catgtgtaac cctttgataa aaatacgcat
      361 ttgattgctg tcgaaggtac ctggcaccga ttgatcatac ggggttttta gagatgatct
      421 tcattcataa caaaaaggtt gtttgttaaa tgtatatgtt atataattaa cctataaggt
      481 aaatggataa catatatgtt taatcatctg caacagcgtg aaatttttca tctgatgttt
      541 ttgcggcact tttgtcccaa aatcaaaacc aacaccctgt ccctcaaagg gggcgtcaat
      601 ctgcgatttt ttttcaacag ccatcgctat tccgaggacc tggatctgga cataaaagac
      661 atcgaaacat ttcagctcaa ggatatcgtg atgggcattc tggagtcccg ttccctggcc
      721 ctgacactca gaccattcca cataaacaaa atcattccac cggatatcaa cgttgccaaa
      781 caaacggaaa caacacagag attcaaagtg catctcatca cagcatcgag cgaaaacctt
      841 tttacaaaaa ttgagttttc aaagcgacca atgcgcgatg atattgttgt ggaaagggtc
      901 cgtgatgacg tggtctttcc ctataaaatt caaactctat ccataccaca ttacacggcc
      961 caggcggcat ttctgcagaa aataatagcc ctcgcagaac gaggcaaaac acaggcacgc
     1021 gacatttacg atcttgcttg gctcaaggca tttgttgata gccaggcatt aaactgcagg
     1081 catcgtctgg gcacagaaat ttgtgcaaaa gccaagggaa atatttttaa aatctcattc
     1141 gctattttca gggacacggt gcaaaactat ttgaacgaag aggaaaggtt gctctaccag
     1201 catcccgaca gttgggaaga actgcaactg cgagtctgtc aaatcataga agagattgtc
     1261 gagaacaggt tatgaaagcc aattttgcgc aaatgattct cgttcacgag caaaccgtct
     1321 tcaccacgcg tgaagccgct gaacggtgtc acacgtcgct ggcatcggcc tcacggggtt
     1381 tggccctgct gacaaaacgt ggtttgctga cgcacgtcat gaaaagtgtc tgggccaaga
     1441 cttctgacaa gacattttcg gcccaccagg tcattccgca cttgaatcac aagcaccagt
     1501 tttatttgtc gtttaccagt gccctgagcc tgcatggtgt cctctcacag cttccccaga
     1561 gggtgactgt ggcctcgacg gcgcattctg gtatggttaa aacacctgtt ggtgtttttg
     1621 aaattcacca actcgcgcca gaattatttc agggctttga ctgggatagt gcacatcagt
     1681 atttattggc cacgcctgag aaggcacttg tcgattgtct ctatctctcg tttcgcaaaa
     1741 aaaagcagtt cggatatttt ccagaaattg atatgtcttt actcaatcaa aaaaacatca
     1801 tgaagtggat caacctgatt cgggaccagc gaacgaaatc attggtcaag gcaaaactca
     1861 aggaactcct ggtgtgaaag gtgcatttcg aactattgaa agttgccagt ccccgtaaag
     1921 cttccaccta cgctaaagct tcggtggaca ggtcggggca cgagttgact tggtttgatg
     1981 cgcgtggctt atcacggaat ctttgattcg ggccggtcaa gccagacccc tacgtcttgg
     2041 gtgccaaatg cgatgttatg acggagtaat gatgttgcat gtgtaaccct ttgataaaaa
     2101 tacgcatttg attgctgtcg aaggtacctg gcacc
//
LOCUS       DATDUN010000166         1148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_464298, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000166 DATDUN010000000
VERSION     DATDUN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464298"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VJC18_02255"
     CDS             <1..753
                     /locus_tag="VJC18_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44232.1"
                     /translation="LLANYTNYEENVVVLDFIKQQLPNIAVDSELAQQEASTVSNPLY
                     GTTTDFVSAMNTYITDASEKYAEKAADHHGDSIKQNLDGDYDAAQESIGEFSEDIERQ
                     SASKALTELMTQISELISEDGQIRLGNLRDLVTSYLGALDPTIKDDATSLFMCMLFSV
                     DYNLARTLCNDSTFASGLIANIDAGGGVASTLVEEAKALINCTKLEPSEPSYDYTPGD
                     GLPSVTLTYRTFPWLTDDETADLWATHNASLG"
     gene            complement(789..>1148)
                     /locus_tag="VJC18_02260"
     CDS             complement(789..>1148)
                     /locus_tag="VJC18_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HLD44233.1"
                     /translation="SGKDPSKVDRSACYMARYVAKNIVAAGLAEQCELQVAYAIGYPE
                     PVSLMVNTFGTNKVSEEKIESAVREIFSFKPANIIKHLELLRPIYQNTAAYGHFGRDE
                     FSWEKTDKVAELKRVCG"
BASE COUNT          300 a          311 c          268 g          269 t
ORIGIN      
        1 ctgctggcca actacacaaa ctacgaagag aacgttgtgg tgctcgattt tatcaaacaa
       61 cagctcccca acatcgctgt ggattccgaa ttggcccaac aggaagccag taccgttagc
      121 aaccctctgt acggtacaac gactgatttt gtatcggcca tgaataccta catcacagat
      181 gccagtgaaa aatacgctga gaaagccgct gatcatcatg gcgattccat caaacagaac
      241 ttagatggtg actatgatgc ggcacaagag tcgattggtg aattcagcga ggacattgaa
      301 cgtcaatcag ccagcaaggc gttaacagag ctcatgacac aaatctcgga gttgatttct
      361 gaagacgggc aaatccgtct cggcaacttg agggatttgg tcacatcgta cttgggagcc
      421 cttgatccaa cgatcaaaga cgatgccaca tcccttttta tgtgcatgct tttttcggtg
      481 gattacaatc tggcccgcac cctttgtaac gacagcacat ttgcaagtgg gctgattgcc
      541 aacattgacg ctggcggggg agttgcgtca acactcgtcg aggaagccaa ggcactcata
      601 aattgcacaa agctggaacc gtcagaacct tcgtatgact atacaccagg agacggcctt
      661 ccttcagtta cattgactta cagaacattt ccttggctca ctgatgacga aaccgccgat
      721 ctctgggcta cacacaacgc tagccttgga tagaccaccg ctacggagta acacaacttc
      781 cgttttttct acccacacac ccgcttcagc tccgcaacct tatctgtctt ttcccacgaa
      841 aactcatccc tgccaaaatg accgtaggcc gcagtgtttt gataaatggg tctcaggagc
      901 tcaaggtgct tgatgatgtt ggccggtttg aagctgaaaa tttcgcgcac ggcactctca
      961 attttttcct cggaaacttt gttggtgcca aacgtgttga ccatgagaga gacgggctca
     1021 gggtacccga tggcatacgc cacctgcagt tcgcactgct cggccaagcc cgccgccaca
     1081 atgttcttgg ccacgtaacg cgccatgtaa caggcagagc gatccacctt ggaagggtcc
     1141 ttgcccga
//
LOCUS       DATDUN010000167         1075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_547048, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000167 DATDUN010000000
VERSION     DATDUN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547048"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VJC18_02265"
     CDS             complement(<1..1003)
                     /locus_tag="VJC18_02265"
                     /inference="COORDINATES: protein motif:HMM:NF037180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HLD44234.1"
                     /translation="MKEVTLVDVYTIDRDFSEKDWPRMGALLSNSVTQKFSTVQALSP
                     KEFDWAVEIGFLPGVTDNVAHTAKESIEDLLGVPFEAGQGVYSSQIVFVRGALSRDEV
                     AGVSGLLSNALIQRVHIKSHEEFLRDHGMDLVLPKVRLSQKTNIDEISLALSDQEMET
                     LGQKGICNEDGSRRGPLSLSLDDLKIIQEHFAKKGRRPTDVELEMLAQTWSEHCKHTL
                     FSASLDDITEGIYKTYIRRATEVIRKVKGKSDFCVSVFKDNSGGIVFDQDWIITDKVE
                     THNSPSALDPYGGAITGIVGVNRDTIGFGMGAKPVINKYGFCFGLPHDQKALYRSEHK
                     "
BASE COUNT          262 a          309 c          250 g          254 t
ORIGIN      
        1 acttgtgttc cgagcgatac agggccttct gatcgtgggg taggccaaaa caaaaaccgt
       61 atttgttgat caccggcttg gcgcccatcc caaacccgat cgtgtcgcga ttcaccccca
      121 caatgccggt gatcgcgcca ccgtagggat caagcgccga tgggctgttg tgggtttcga
      181 ccttgtcggt gatgatccaa tcctggtcaa acacgatccc gcccgaattg tccttgaaga
      241 cggaaacaca aaaatcgctc ttgcctttga ctttgcgaat cacctcggtt gcccgtctga
      301 tgtaggtttt atagattccc tcggtgatat cgtcgagaga ggccgaaaac agggtgtgtt
      361 tgcaatgctc cgaccaggtc tgcgccaaca tctccagctc cacatcagtc ggtctgcggc
      421 cttttttggc aaaatgttcc tgaatgatct tcagatcgtc caacgacaac gacaggggac
      481 cacgacgcga accatcctcg ttacaaatac ccttttgtcc caaggtctcc atttcctgat
      541 cggaaagcgc aagggaaatt tcatcaatgt tcgtcttttg agatagcctc actttgggca
      601 aaaccaaatc catgccgtga tcgcgcagaa attcttcatg cgatttgatg tgcacgcgtt
      661 gtatcaaggc attggaaagc aagccactca caccggccac ttcatcacga ctgagtgccc
      721 ccctgacaaa aacaatctgt gacgaataca caccctgccc ggcctcaaag ggaacaccca
      781 acaaatcctc aatgctttct ttggccgtgt gtgccacatt atcggtcacc ccgggcaaaa
      841 aaccaatctc aacggcccaa tcaaactcct tgggagacaa cgcctgcacg gtggaaaatt
      901 tttgggtcac cgaattggac aacaacgctc ccatacgcgg ccagtccttt tctgagaaat
      961 cacggtcaat cgtgtacaca tccacaaggg tgacctcttt cacctgtttg gcaaagcctg
     1021 cagcatggat ccgccgctta cagactgcgg cgcgggtgtc tgtgatgatc gtttg
//
LOCUS       DATDUN010000168         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_214911, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000168 DATDUN010000000
VERSION     DATDUN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214911"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="VJC18_02270"
     CDS             complement(<1..1657)
                     /locus_tag="VJC18_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HLD44235.1"
                     /translation="MIRPAIVTTPLPSMFASASALDATTPGPLREVVAGANPDITRHP
                     QLQKIVASSDRGGGRLRHTYFTARSARQSAKGQRGYIHPFFAGTTLMIGACTYYLVTH
                     EWDRTSGGLLLLDLMSGLALGLVMINRIGLHTEMRVVLSKIREATDNETREILEANLR
                     LYNGVALEWLPIYKMDLRQFPLHRISLLGLDIRQTLLPADCAKIDFTGAKLDRSQVKH
                     IIKHRGQLCGADLSGINLKGIDLSKADLRFVNLQGTNLKKTRLPQDCAGIDFTGALLG
                     RTEVMHIIEHNGALRGANLEGVDLSGMNLDEQDFTDVNLNGVILRNSVLSQSALKGVS
                     LKNVDLRGAVLPDDCSGINFAGALLTDLQVWQILHHGGTVREAQVNDVDFSGRCLESP
                     NFVQVVFKGADLMGASLDGAKFDLASLEDANMIDTKLRGASFTYANLKSAKLVSAQLS
                     GSKLMHADLRRADLSYADLSGADLTEAKLSYAVLEGTRLAGAKLPEDCSGVDARRAIL
                     SNDQREWIRDRGGLVAEARVSAESAGLPETAREARKPPSRQRER"
     gene            1818..>2876
                     /locus_tag="VJC18_02275"
     CDS             1818..>2876
                     /locus_tag="VJC18_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44236.1"
                     /translation="MFRPIKPFVDQFLLCPEPGTDMFLAPLPPSVSARMDVLDTYGTT
                     NMAFAIKDDVPRPRITSRIFERKPQQISDLSAVTPLAPTTEKPITTSDTDPAPSARVY
                     SQQELSDEIVLQLMLLLADDVLKEAERRLSDRASKTANDIESTELEQQRSLLHRAFRE
                     IPKKLSEPSALNDRETRLRIIRSTLDYVQAIPALRKKYSAYIEPIKRFLHIGSGEVGF
                     VSASRHPVALQPRNSGTPEPDHIPLMQFAGKTWQEICELCNFNPRVYQAAWYEALLAR
                     AAQGTDRAYLGAPYQVGKTYACLPLAVALGTTCFPGKTILIIAPQRVVKETIIEDLRR
                     AHAGLKIGIIDADHKDFGC"
BASE COUNT          712 a          814 c          636 g          714 t
ORIGIN      
        1 ccctctctct ttgacgactg gggggcttcc gggcctctct tgccgtttcc ggcaatcctg
       61 cagattctgc agaaacgcgt gcctcggcca ccaaaccacc cctatctctg atccattcac
      121 gctggtcgtt cgagaggata gcccgtctgg catccacacc ggaacaatcc tctggcaatt
      181 tggcacccgc aagtctggtc ccttcgagca cggcataact gagcttggct tccgtaagat
      241 cagcacccga aaggtccgca taactcaggt ctgccctcct aagatcggca tgcatcagtt
      301 tcgatccact gagttgcgcc gaaaccagct ttgctgattt aagattggca taggtaaaac
      361 ttgccccccg caattttgtg tcgatcatgt tcgcgtcctc aagactcgcc agatcaaact
      421 tcgctccgtc tagagaggct cccattaaat ccgctccctt aaaaacaact tgaacaaaat
      481 ttggactctc cagacagcga cctgagaaat caacatcgtt cacttgggct tctctaacag
      541 tacccccgtg gtgcaaaatt tgccatacct gcaaatcggt gagcagggcg ccagcaaaat
      601 tgattcccga acaatcatca ggcaacacgg cccccctcaa atcaacattt tttaaactga
      661 cccccttgag cgctgattgt gataacacgg aattcctgag gataaccccg ttgaggttga
      721 catcggtaaa atcctgttcg tcgagattca ttccggagag atcgacgcct tccagattag
      781 cacccctgag cgccccgtta tgttcaatga tgtgcattac ttcagtgcgg cccaacagtg
      841 cgcctgtaaa atcaattccc gcacagtcct gcggtaatct ggtttttttg agattagtcc
      901 cctgcagatt cacaaatcgg aggtcagctt ttgaaagatc gatccctttt aagttgatgc
      961 cgctcaaatc cgcgccacat aattgtcccc gatgtttgat aatgtgtttc acttgggagc
     1021 gatccagctt ggccccggta aaatcgatct tcgcacaatc agcaggcaac agggtctgtc
     1081 tgatatccaa tcccaacaac gaaattctgt gcaacggaaa ctgtctcaaa tccattttat
     1141 agataggaag ccattccaat gcaacgccat tatacaagcg caggttggct tccagtattt
     1201 cacgcgtttc gttgtctgtt gcctcgcgaa tttttgacaa gacaacacgc atctctgtat
     1261 gtagcccaat cctgttaatc atcaccagac ccaaggccaa gccagacatc aaatcgagca
     1321 ataacagtcc accactggtg cggtcccatt cgtgtgttac gagataatag gtgcacgcac
     1381 caatcatgag ggtggtgcca gcaaaaaaag gatgaatata tcctctctgc ccttttgcgg
     1441 attgtcgcgc gcttcttgcc gtaaaatacg tatgacgaag tcttccacct cccctatcgc
     1501 tactggcaac tattttttgg agttgtgggt gtcttgtaat atctggattg gcaccagcaa
     1561 caacttccct gagaggtcct ggggttgttg catcgagtgc agacgcacta gcaaacatgc
     1621 ttggcaaagg cgttgtgacg atggctggtc tgatcatggt tttttctccc taccattcca
     1681 tcgtcaatcg atcagcattt gttgcgttgc cagcgaattt tccgtgtccg ccaggacacg
     1741 gattttcatg gcctcacaca acattagtct cactctgccg ataattgtgt tgttagcgta
     1801 accccgcgga gacaacattg tttcgaccca ttaaaccatt tgttgatcaa ttcctacttt
     1861 gtccagagcc cggcaccgac atgttcctgg cccctctccc cccttctgta tctgccagaa
     1921 tggacgtact cgatacgtac ggcacaacaa atatggcctt tgccataaag gatgatgttc
     1981 cgcgcccccg tatcacctct cgtatctttg agagaaaacc ccaacagatc tctgatttgt
     2041 cggcagtaac accactggca cccacaacag aaaaaccaat cacgacttcc gatacagacc
     2101 cagctccttc cgcacgcgta tattcccagc aagaactcag cgacgagatt gttcttcagt
     2161 tgatgcttct tttggcagat gatgtactca aagaggccga acgccgtcta tcagatagag
     2221 ccagtaaaac ggcaaatgac attgaatcga cggaactgga acagcaacga tcactgctcc
     2281 atagggcgtt tcgcgaaatc ccaaaaaaac tgagcgagcc ttcagctctt aacgatagag
     2341 agacacgcct tcgaatcatt cgcagtacgc tcgattatgt tcaggcaatt cccgctctgc
     2401 gtaaaaaata ttctgcatat atcgaaccca tcaagcggtt tttacacata gggtcaggag
     2461 aggtcggttt tgtttccgcc tcacgacacc ccgttgccct acaaccaagg aacagcggca
     2521 caccggaacc cgaccacatt cctcttatgc agtttgccgg taaaacctgg caggagatct
     2581 gcgagctatg caattttaat ccgcgcgtct atcaggccgc gtggtatgag gccctgttgg
     2641 cacgggccgc gcaaggtact gatcgcgctt atctgggcgc cccgtatcag gtgggcaaga
     2701 cgtatgcgtg cctgcctctg gctgtggccc ttggcaccac ctgttttccc ggcaagacaa
     2761 tcctgatcat cgccccacaa cgcgtggtca aagaaaccat cattgaggac ctgaggcggg
     2821 ctcatgccgg tttaaagata ggcatcatcg acgcagacca caaggatttt ggatgt
//
LOCUS       DATDUN010000169         1275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_589867, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000169 DATDUN010000000
VERSION     DATDUN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589867"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VJC18_02280"
     CDS             <1..669
                     /locus_tag="VJC18_02280"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor 1 family
                     protein"
                     /protein_id="HLD44237.1"
                     /translation="KKTMFIANLRFSFYLECILKPRNKYVLDESGSTFYKRAMDEIER
                     QIKELKILSQQKQTATQTQSTPQQHTHMTSQGQTPAPLPPIYQKKLALQKKHRSSCLI
                     PISCGAGCLVLIAFVVMILVIVFGFMKSSDVYKTALDRAQHSTGVIEVLGEPIESGWF
                     VSGSINTSGSTGNASLEIPITGPNGAGTIHVQAQKSQGKWQFSSLVVEIKDKGLTINL
                     NAGQ"
     gene            complement(890..>1275)
                     /locus_tag="VJC18_02285"
     CDS             complement(890..>1275)
                     /locus_tag="VJC18_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44238.1"
                     /translation="IANTTTSTLTINYEFTAGASNAPSLTVSGCPVTMDINTSVPIDF
                     GYADGDGNITTLWETVTLAGESVTASGDVSTYMTGTSGTYSTYIEYTGDNDESQTLVY
                     TFWIVDADGNSSNTETCTTELLPNI"
BASE COUNT          340 a          315 c          306 g          314 t
ORIGIN      
        1 aaaaaaacaa tgttcatagc aaatctccgt ttctcctttt accttgagtg cattttgaag
       61 ccaagaaata aatacgttct tgatgaatca ggctccacat tctataaaag agccatggat
      121 gaaattgaac gccagatcaa agaactgaaa atactatccc aacaaaaaca gactgcgaca
      181 caaacacagt ccacaccgca acagcatact cacatgacat cgcaagggca gacgccagca
      241 cccctgccac ccatctatca aaaaaaactg gccctgcaga aaaaacacag gtcctcgtgc
      301 cttatcccca tcagttgtgg cgctggttgt ctggtgttga ttgcctttgt cgtcatgatc
      361 ctcgtgattg tttttggttt catgaaatca agcgatgtgt acaaaacagc cttagacaga
      421 gcgcaacaca gcacaggggt cattgaggtt ctgggagaac ccattgagtc gggttggttt
      481 gtgagcggga gcatcaatac atctggatcc actggcaatg ccagccttga gattcccatt
      541 acaggaccaa acggtgccgg cactattcac gtccaagctc aaaaaagtca gggcaaatgg
      601 caattttcct cgctggttgt ggaaatcaaa gacaaggggc tgacgatcaa tttgaatgcg
      661 gggcaatagg aacgtgagtc ggaaatcgtg ggtcgtctgt gcccctgagg gtatgaatcg
      721 aaatgtgttt tgtcatccct gcgcaggcag ggatccagaa ttgacgtaaa tatggatccc
      781 agctttcgct gggatgacaa attttattat tcgacattcc ttgtttcagc cgtaggactg
      841 atcccgtttg cgggacgatc cacgatacac gattcacgat ccacgttcct catatattcg
      901 gcaacagctc cgtcgtgcag gtctcggtgt tgctcgagtt tccgtcggca tccacaatcc
      961 agaaggtgta cacaagcgtc tggctctcat cgttatcacc cgtgtattct atgtaagttg
     1021 aatacgttcc cgacgtgcct gtcatgtatg tcgacacatc gcccgacgcc gtaacagatt
     1081 ctcccgcgag tgtgacggtt tcccaaagag tcgtgatgtt gccatcacca tcggcatacc
     1141 caaaatcaat cggcactgag gtattgatgt ccatggtgac gggacagcca gacacagtga
     1201 gtgatggcgc attggacgcc cccgctgtaa actcgtagtt gatggtcagt gtggatgtcg
     1261 tggtgttcgc aatga
//
LOCUS       DATDUN010000170         1683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_257773, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000170 DATDUN010000000
VERSION     DATDUN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257773"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..616
                     /locus_tag="VJC18_02290"
     CDS             209..616
                     /locus_tag="VJC18_02290"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HLD44239.1"
                     /translation="MFKRLRGVQNQRGMSLIEIMIVLGIIATATAAIMSAVLPKADIA
                     KRDQTKTTMSSLKGDLQQYKMEKKKYPSNDEGLSALVEAGYIEEVPVDGWGNDFVYEY
                     PGSHGKKYDLCSPGKDEDSDEDDMCNYDKDTEE"
     gene            796..1431
                     /locus_tag="VJC18_02295"
     CDS             796..1431
                     /locus_tag="VJC18_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44240.1"
                     /translation="MTLIEVMLSLAIIAGIMAFVVGAAGNSSGNELRRLAGQMVRTIK
                     HAYMQAAIANGYYRLAIDLSAQPQTYQLEYSAKPFYIVNADDEKEELRKKNEERLVGE
                     EEQSLAKAVGVGEFSEAEDDLVPRTEISADIKIMDVRTEHDQDAVAEGITHLYFFPKG
                     YTEFSIIHLSDAEETKFITLIVNPLTGEVDVVEDFVEHEDALQRLGLSEEE"
     gene            1437..>1683
                     /locus_tag="VJC18_02300"
     CDS             1437..>1683
                     /locus_tag="VJC18_02300"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLD44241.1"
                     /translation="MRKILKNHLGFSLIEILIALGILAGSVGVFVSRAHNSSRMTIED
                     QRELTGVMLANNKMIEVEAEIGDDTARGKFPDEKEEAG"
BASE COUNT          452 a          343 c          471 g          417 t
ORIGIN      
        1 ctcaccccga tcatgctgat ttttatgggg gccatgatcg ccctgatcgt ggccgccgtc
       61 ctcatgccga tcctgcagct ttccggttga ttttttacga tgaatcgtta tggggggggg
      121 tgatttcgta gcagttgcgc aatatttttt ttttggttaa aaaggcaaag tataaaccgt
      181 gctgggaaag ccatgaaagg agtttgtaat gtttaaaagg ttgagaggtg ttcaaaatca
      241 acggggaatg tcattgatcg aaatcatgat tgttctggga attatcgcga cggcaacggc
      301 agccatcatg agcgctgtat tgcccaaggc ggacatagcc aagcgtgacc aaacgaagac
      361 caccatgagt tcactcaaag gtgacctgca gcagtacaaa atggaaaaga aaaaatatcc
      421 ctccaatgat gagggcttgt ctgccctggt ggaagcaggt tatattgaag aagttcccgt
      481 tgatggttgg ggcaatgatt tcgtgtatga atatcccggc agtcatggca agaaatacga
      541 tctttgttct cctggtaaag atgaagacag cgatgaagac gatatgtgta attacgacaa
      601 ggacaccgaa gaataagcca gtgagtttta tgtttaaagg caagatcacg gtttttgagt
      661 acgagaagaa ttggtcgccc cttgatgtgt tgatggtgat tttgatcgtc gtgggtgcgt
      721 tagccgcggc cctgtctcct ttctactgaa cccctaatgt ccttctgtct taacaagcga
      781 agtctccaca gtggcatgac cctcattgag gtgatgctct cgttggcgat catcgcgggt
      841 atcatggcct tcgttgtagg ggccgctgga aacagctccg gcaatgagtt gaggcgcctg
      901 gctggtcaga tggtcaggac aatcaaacac gcatacatgc aggcggcgat cgcgaatggc
      961 tattaccgtc tcgccattga tctgtcggca cagccgcaaa cgtatcagct cgaatattca
     1021 gccaagcctt tctatattgt gaacgcggac gatgaaaaag aggaactgag aaaaaagaac
     1081 gaagaaagat tggtggggga ggaagaacaa tccttggcca aagcggttgg cgtgggagaa
     1141 ttttcggaag cagaagatga tttggttccg cgcacagaga tttccgcaga catcaaaatc
     1201 atggatgtgc gtactgaaca cgatcaagac gctgtcgccg aagggatcac gcatttgtat
     1261 tttttcccca aaggatacac tgaattttca atcattcatt tgagcgatgc agaagagacg
     1321 aaattcatta cgctgattgt gaaccccctg acgggtgaag tggatgttgt ggaggatttt
     1381 gtagaacacg aggacgcctt gcaacggttg ggtttgtcgg aagaggaatg acgttcatgc
     1441 ggaagattct taaaaatcat ttagggtttt ccctgattga aattctgatt gccttaggca
     1501 tcctggcagg ctcggtgggg gtgtttgtct cgcgcgcgca caattcaagc cgcatgacca
     1561 tagaagacca gagggaactt acgggcgtca tgttggccaa caacaagatg atcgaggtcg
     1621 aggccgaaat aggagacgat acggcacggg gtaaatttcc cgatgaaaag gaagaggccg
     1681 gcg
//
LOCUS       DATDUN010000171         1536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_8805, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000171 DATDUN010000000
VERSION     DATDUN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8805"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..726)
                     /locus_tag="VJC18_02305"
     CDS             complement(220..726)
                     /locus_tag="VJC18_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44242.1"
                     /translation="MSKHIINMLYNPGSISTAGTGQGTDDQETRVLLTGNNLSTCTSL
                     GHRDYHQLEELEALVQADDEMGILSRLRSLKEQLRDGMATELRPVLLKEFNQYMSHAL
                     QHHPKSQRIFQNSLEVILTIKKDIDDSPQTNGYISQLEWVELERSVYCLSEWEDGMKS
                     NQEFTFLS"
     gene            1053..1379
                     /locus_tag="VJC18_02310"
     CDS             1053..1379
                     /locus_tag="VJC18_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44243.1"
                     /translation="MINKAPHKHHHLLQFAKTKGFLSPLWLLPNTDPDGVQRAFQELE
                     SFLHNAYQQNELTAMELSDVDEFIWKLKKTYAFKGWEGIAPYLLTFPTHSTQSANEGL
                     YKPKFG"
BASE COUNT          399 a          362 c          349 g          426 t
ORIGIN      
        1 ttcgcgcaaa aacagctcgc cgtgtcaccc aaaaaaggtc accttgcgga aggtttacga
       61 ataggctttt agtgaagggc tcttaatgcc cttgattttc agacaagcta tcgcaagtca
      121 agaaaaaggg ggtgtgggga aaaaacgcgc ccccgatttt tgcattgttg tctccaaaca
      181 tggtgttcgg aggcgcgtca gagaaaaata aaatgatgtt cagcttaaaa aagtaaattc
      241 ctggtttgat ttcataccat cttcccactc agacaggcaa taaacagagc gttccagttc
      301 gacccattcc aactggctga tgtacccgtt ggtttgtggg gaatcgtcga tgtctttttt
      361 gatcgtcaaa atcacttcaa ggctgttttg aaaaatgcgc tgtgactttg ggtgatgttg
      421 caaggcatga gacatgtatt gattgaattc cttaagcaat accggtctga gttctgttgc
      481 catcccatca cgaagttgct ccttgagtga acgtaaacga ctcaggattc ccatttcgtc
      541 atctgcttgt accaaagctt cgagctcttc aagttggtga tagtccctgt gtcccaatga
      601 ggtacaggtg cttaagttgt tgccggtcaa taataccctg gtttcttgat cgtcggtgcc
      661 ctggccagtc cctgccgtgc tgatcgatcc cgggttgtac aacatgttga tgatgtgctt
      721 gctcattcag gctccttcag ttttaaccaa gccgatcgac agaaaatcga acttggcaat
      781 atttagagga gaataacaaa ggatgccgta ccactgtatt gtgaagcaat catagtgcca
      841 aagcgggaac caatttccac ggctaacggc ttgaaattgt taagccgagc gcccttagcc
      901 agttatccac agtcaaaacc cccgacactt ttatcaaaat ggggaatcat attgagaaat
      961 gaatttaatg cactgtatca aagccgtgcc aatgctgagt ttccttttgc tattttcacc
     1021 aaattcaatt atatgaggca tgagaggcgt ctatgatcaa caaagcgccg cacaaacacc
     1081 atcaccttct tcagtttgcc aagaccaaag gctttctgtc gccactgtgg ttgttgccca
     1141 acacggatcc tgatggtgtg cagagggctt tccaagagct cgagtctttc ctgcacaacg
     1201 cgtatcagca aaatgaactg acagccatgg aactgagtga tgtcgacgag ttcatctgga
     1261 aattaaaaaa gacgtatgcc ttcaagggtt gggaaggcat agctccttac ctgttgacgt
     1321 ttcccactca ttccacgcag agtgcgaacg aaggtctgta taaacctaaa ttcggttagc
     1381 ctcatttagc agtccgttgc ttttgtccaa tctctgcgtt ggtgcgtctt tcccgtcgct
     1441 caacgtacat ttgagtacgc ctccctccgg gagaagccgc acacgccttg atcttcaacg
     1501 atttgatcgg ttttctaaat tccgacttgc ggaatt
//
LOCUS       DATDUN010000172         1048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_547357, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000172 DATDUN010000000
VERSION     DATDUN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547357"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..>1048
                     /locus_tag="VJC18_02315"
     CDS             166..>1048
                     /locus_tag="VJC18_02315"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HLD44244.1"
                     /translation="MNRTHFGCFQMGFEKFHTNYLMFGFMQIVSLISLTACQQDPIAL
                     TQFPDLGVNDFGMGDSNDDFDSKDGGGRDWINVDDSGKCIPEPKGEVCDGRDNDCNDK
                     VDDVDPIKLQSDPNNCGACQKVCSFPNAFAKCSQGECLMDSCAPGYFDIDTSKDGCEY
                     QCLVTNGGIEICDHVDNDCNGVIDDNFDLTSNPEHCGTCGNSCAFGNASASCENSTCV
                     QGECLSGFKDLDGLPGCEYQCPIWPIQNPDESLCDGQDNDCDGDIDEDYTGTPCGSDV
                     GECTPGINVCIVGSLFCQ"
BASE COUNT          289 a          195 c          266 g          298 t
ORIGIN      
        1 gtggatttgg ttgtcagctc gttgccaagc acaacatttt gcatggaatc agtggggtat
       61 tactttggtg cgggctaggt cttctgatat attcactaag gcgtagattc cgatatgaca
      121 ggaaaatcca tccagataaa ctgcataatt aaaagggcta tatagatgaa tcgtacacac
      181 tttggttgtt ttcaaatggg ttttgaaaaa tttcatacca actaccttat gtttggcttt
      241 atgcaaattg tgagtttaat ttcgctcact gcgtgtcagc aggatccgat agcattaact
      301 cagtttccag acttgggagt gaatgatttt gggatgggcg actccaatga tgattttgat
      361 tcaaaagatg gaggaggacg cgactggata aacgtagatg atagcggcaa atgcatccct
      421 gaacccaaag gggaggtttg cgacggaaga gacaacgatt gcaacgataa ggtcgatgat
      481 gttgacccca tcaaacttca aagtgatccc aataattgtg gagcatgcca aaaggtctgc
      541 agtttcccca atgcctttgc taagtgttct caaggcgagt gcctcatgga ttcttgtgca
      601 ccgggctact ttgatatcga tacatccaaa gacggctgtg aataccaatg tttggttacg
      661 aatggcggga ttgaaatttg tgatcatgtt gacaatgatt gcaatggtgt catcgacgat
      721 aatttcgacc taacttcaaa cccagaacat tgcggcacct gtggaaatag ctgcgccttt
      781 ggaaatgcga gcgcctcttg tgaaaacagc acctgcgttc aaggcgaatg tttaagcggg
      841 ttcaaggatc tagatggtct gcctggctgc gaatatcaat gtccgatttg gccaattcag
      901 aatcctgatg aaagcctatg tgatggtcaa gataatgatt gcgatggtga tattgatgaa
      961 gattatacag gaacaccatg tgggtctgat gttggagagt gtacgccagg catcaatgtt
     1021 tgtattgttg gcagcctgtt ttgccaag
//
LOCUS       DATDUN010000173         1004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_506374, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000173 DATDUN010000000
VERSION     DATDUN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506374"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VJC18_02320"
     CDS             <1..187
                     /locus_tag="VJC18_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44245.1"
                     /translation="LPFLGSRKSLTNDSCADKQLAENEKMKAKLSQSSQLFTKKLWPK
                     KSKTHMPLSGGSRINNL"
     gene            200..>1004
                     /locus_tag="VJC18_02325"
     CDS             200..>1004
                     /locus_tag="VJC18_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44246.1"
                     /translation="MSPSPSVAPALAFPLPQSIAYSALGLSPKALGLVKAGARAMGET
                     INTQQAFRVVRDTFGLGKDIRTGYGREAHGVLMEKFFPGFTTFTRTVDVVHPVQVQIK
                     DEADGDTTKIWVEGYGNVSVRHDGIDTPESNPSEKLSKVVNYIFAYLQEEHAIPDSDM
                     TAAKAVIQARVVYLGKLAGLVNKAFMGWSEFVSLAPAYARVAGAAHLCDVLDYADKYG
                     RVLGRFMAGMPGEQNDLFDGFITSEVYNVMKAGGMAERETYRQKLGPHSG"
BASE COUNT          267 a          218 c          275 g          244 t
ORIGIN      
        1 actgccgttt ttaggttcaa ggaaatcgtt aacaaacgat tcttgtgctg acaagcaatt
       61 agcagaaaat gaaaaaatga aggcaaaatt atctcaatca tcacagctct ttacaaaaaa
      121 actctggcca aaaaaatcca aaacacatat gcctctgtca gggggcagta gaatcaacaa
      181 cttatgacag ggggaccaca tgtcaccatc accttccgtt gcgccggcct tggcgtttcc
      241 attaccacaa tcgatagcct attccgcgtt gggtttatca cctaaggcgt tgggtttggt
      301 caaggcgggg gccagggcta tgggagagac catcaacaca caacaagcct ttcgtgtcgt
      361 tcgggatacc tttggattgg gaaaggacat tcgtacgggc tacggtcgtg aggcgcatgg
      421 ggtcttgatg gaaaaatttt tccccggatt caccacgttc acaagaaccg tagatgttgt
      481 ccatcccgta caagtacaaa ttaaggatga ggcggatggt gacaccacca aaatctgggt
      541 tgaaggctat ggcaacgttt cggttcgcca cgacggcata gacacacccg aatcaaaccc
      601 gagtgaaaaa ttatcaaaag tcgtgaatta cattttcgcg tatcttcagg aggagcatgc
      661 tattcctgat agcgacatga cagccgcgaa ggctgtgatt caggcgagag ttgtgtattt
      721 ggggaaactg gcagggttgg tcaacaaggc atttatgggt tggtccgagt ttgtcagttt
      781 ggcacccgcc tatgcgcgcg tggcaggggc ggcgcatctc tgtgatgtgc ttgactatgc
      841 cgataaatat ggccgggtgt tgggtcgttt tatggcaggt atgccaggtg aacagaatga
      901 tcttttcgac gggttcatca cgtcggaggt gtataatgtg atgaaggctg gtggcatggc
      961 agagcgtgaa acctatcggc aaaaactggg accgcactct gggt
//
LOCUS       DATDUN010000174         3564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_174075, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000174 DATDUN010000000
VERSION     DATDUN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174075"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1965)
                     /gene="nuoD"
                     /locus_tag="VJC18_02330"
     CDS             complement(151..1965)
                     /gene="nuoD"
                     /locus_tag="VJC18_02330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HLD44247.1"
                     /translation="MTSVSQHLNKTLPDAIVSSCDDVLSRHEVLCILPQRLHDVVLVL
                     KKELFFDVLVDVFSIDWQAKKTKRFEVVYLFKNSRDQLRVCLRVSPVRSDKPSVSSLA
                     DIYSAANWAERECYDLMGIFFEGHPNLQRLLMWDEFEGHPLRKDYPLNLRQPIPKTKE
                     NLFEPFQISNNKGRSRNIEKLSSLRTQGSRTNICMDPSFRWDDKSLFISTSNIYHADS
                     EHLIVNLGPSHPAFHGALRTQVRLDGEVIVQARSEIGYLHRCFEKHAEHASYTQIIPY
                     SDRLNYVSALMNNVGYCKAVEDLMQITIPERAMCVRVLLCELSRVMDHLVCVGTNLVD
                     MGALTNFWYAFNAREKITRLIEKVTGARLTYAYTRVGGLAQDLYPEFLGELKPVLKEV
                     RQAIKDMTGLVVKNKIFLDRTQGVGAVSAKQALDWGFTGPCLRACGLPHDLRKTEPYY
                     QYDTYDFDIPVGQYGDTYDRIMVRFEEMEQSLRIVEQCLVRMPSGPIMIDDRKLCLPA
                     KQETYTNIEALMQHFKIIMHGIKPPPGEVYSRTEAANGELGFFLVSEGGSSPYRIKVR
                     PPCFYLFAAFEHMIRGHMIADAVAILGSLNIVVGECDR"
     gene            complement(1962..2426)
                     /gene="nuoB"
                     /locus_tag="VJC18_02335"
     CDS             complement(1962..2426)
                     /gene="nuoB"
                     /locus_tag="VJC18_02335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HLD44248.1"
                     /translation="MDNMLTTRLKDIVHWGQKYSLWPLPYGTACCAIEFMGTVSAAFD
                     ISRFGAEVVRFSPRQSDLLLVMGTINYKQAPVLKQIYDQMCEPKWVVAVGACASSGGF
                     YDNYSVLQGIDQIIPVDVYVPGCPPRPEQILHAIIALQKKIDPNGFVDRVKT"
     gene            complement(2596..3006)
                     /gene="ndhC"
                     /locus_tag="VJC18_02340"
     CDS             complement(2596..3006)
                     /gene="ndhC"
                     /locus_tag="VJC18_02340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HLD44249.1"
                     /translation="MIEYLPVLIMFGFAAFVAGVFMLVSFVLGPKKPSVVSQSTYECG
                     VTPTGDARKPVSVKFYLTAILFIVFDVEIIFLYPWAVNFQKAVREGRGWHQLLVMGIF
                     FFLLVLGLFYEWRKGALEWGGDLRAHERTSVREG"
     gene            3127..>3564
                     /locus_tag="VJC18_02345"
     CDS             3127..>3564
                     /locus_tag="VJC18_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HLD44250.1"
                     /translation="MKIHEYQAKTLFKQFGIPVPEGIVCKTADDVFTAAQKMGAVLVV
                     KAQIHAGGRGKGQVLDRTNQNKLVLDGGVKVVKSAEEAKAIASQMLGNTLVTHQTGPS
                     GKTVKTVLVETACHIKKEYYLGLVLDRTKSRITFLASAEGGMDI"
BASE COUNT          963 a          943 c          827 g          831 t
ORIGIN      
        1 gatccgacac ggcctcctgt gcttccaaag acacatgccc cagttgactc tgcaccaacc
       61 agagcacggg gagcaaggcc gtctgatgat ggggagtgcg atccaacatc ttttgaatct
      121 ctgcctgcag tggttggtca aaaacgaatt tcatcgatca cattctccca cgacaatgtt
      181 gagtgatccc aaaattgcca ccgcgtcagc aatcatatgc cctcgaatca tatgttcaaa
      241 ggccgcaaat aggtaaaaac aaggtggcct caccttaatg cgatacgggc ttgatcctcc
      301 ctccgaaacg aggaaaaatc ccagttcccc attagcggct tctgttcgtg aatacacctc
      361 accgggtggt ggcttgatcc cgtgcatgat gattttgaaa tgctgcatca gggcttcaat
      421 gttcgtgtag gtctcctgct tggcaggaag acagagttta cgatcgtcaa tcatgatggg
      481 ccctgagggc atgcggacta ggcattgttc gacgatccgc aggctttgct ccatctcctc
      541 aaaacgcacc ataatgcggt cgtaggtgtc cccgtattgt cccacgggga tgtcaaagtc
      601 ataagtgtcg tactggtaat aaggttctgt ctttcgcagg tcgtgcggca gtccgcatgc
      661 ccgcaaacag ggtcccgtga acccccagtc cagcgcctgt tttgcagaca cagcacccac
      721 tccctgcgtt cgatccaaaa aaattttatt cttcacaacg aggcctgtca tatccttaat
      781 cgcctgcctc acttctttga gcaccggctt taactcaccc agaaactcgg gataaaggtc
      841 ttgcgccaag ccaccaacgc gcgtgtaggc ataagtcaat cgggcacccg tcaccttttc
      901 gatgagacgc gttatttttt cgcgtgcgtt gaacgcgtac cagaaattgg tgagagcccc
      961 catgtccaca agattcgtcc ccacacaaac cagatgatcc atcacccgcg acagttcaca
     1021 caacaacacg cgcacacaca tggcccgttc tggaatggta atctgcatca aatcctcgac
     1081 ggccttgcaa tacccaacat tattcatgag ggccgatacg taattgagac gatcggaata
     1141 ggggatgatc tgcgtgtagc ttgcgtgttc ggcgtgtttt tcgaaacacc ggtggagata
     1201 acctatttcg gaacgagcct gcacgatcac ctcgccatca agccgtacct gagtccgcag
     1261 ggcaccgtga aaagcaggat gactgggacc caggttgaca ataagatgct ccgaatcggc
     1321 atggtaaata tttgatgttg aaataaagag gcttttgtca tcccagcgaa agctgggatc
     1381 catacatata tttgttctgg atccctgcgt tcgcagggat gacaactttt cgatattcct
     1441 tgatcggcct ttgttattcg aaatttgaaa aggttcaaat aaattttctt tcgttttggg
     1501 aatgggttgc cgcaagttca agggataatc cttgcgcaaa gggtgcccct caaactcatc
     1561 ccacatcagc aggcgttgca aattcgggtg tccctcaaaa aaaatcccca tcaggtcata
     1621 acactcgcgt tccgcccaat tggcggctga ataaatatcg gccagagagg acacagaagg
     1681 cttgtcagaa cgaacgggtg ataccctaag acaaactcga agctgatctc tggaattctt
     1741 gaacagataa acaacttcga atcgctttgt tttttttgcc tgccaatcaa tggaaaacac
     1801 atccaccaac acgtcaaaaa acaattcctt cttgagcacc aacacaacgt catgcaggcg
     1861 ctggggcaaa atgcaaagca cttcgtgacg actgagcaca tcatcgcaac tcgaaacaat
     1921 tgcgtcgggc agggttttgt tgagatgttg actgactgat gtcatgtttt aactcgatcc
     1981 acaaaaccat tgggatcaat ttttttctgc aaagcaatga tcgcgtgcag aatctgctct
     2041 ggtctggggg ggcaacccgg cacataaaca tccacgggga tgatctgatc gatgccctgc
     2101 aaaacactgt agttatcata aaaaccgcct gacgaagcac aggcgcccac ggccaccacc
     2161 catttcggtt cgcacatctg gtcgtaaatt tgcttcaaaa cgggggcctg cttataattg
     2221 atggttccca tcaccaaaag cagatctgat tgacgtggcg aaaaacgcac cacttcggcg
     2281 ccaaaacgcg aaatatcaaa tgccgccgaa actgttccca taaactcaat ggcacaacag
     2341 gccgtgccat acggcagggg ccacagcgag tatttctgcc cccagtgaac aatatccttg
     2401 agacgggttg tgagcatgtt atccatcaat aatcattgtc cctttgtggg tcgtgggtcg
     2461 tgggtcgtgg atcgaattca gatgattctg gatccctgcc tttcagccga aggactgatc
     2521 ccgtttgcgg gacacaggga tgacacactt caatattcct tgttcgatat tcgacattca
     2581 aatgtctttc acctttcaac cttctcgtac gctcgtgcgt tcgtgagctc gtagatcccc
     2641 tccccactcc agcgcccctt tacgccattc gtaaaacagt cccagcacca acaaaaaaaa
     2701 gaagattccc atcacaagca attggtgcca ccccctaccc tcgcgaacgg ccttttggaa
     2761 attgaccgcc cacgggtaga gaaaaataat ttccacatcg aagacgatga acaaaatggc
     2821 tgtcaggtaa aatttaacgg acacgggctt tcgcgcatcg cctgttgggg tcaccccaca
     2881 ctcatacgtc gactgactca caaccgaggg tttcttgggt cccaacacaa acgagaccag
     2941 cataaaaacc ccggctacga aagcggcaaa accaaacatg atgaggacag gaagatattc
     3001 aatcataaat gacttcctaa acagaaacga tggaaacgat taagtaattt gtccaatagt
     3061 gtcaatttgt attcaggttt tcgttcacct tgacactcac gttttttctt tattaaagtc
     3121 tggggcatga aaattcacga ataccaggcc aagacgttgt tcaaacaatt tggaatcccc
     3181 gtaccggaag gcattgtctg caaaacggct gatgacgtct ttacggccgc acaaaaaatg
     3241 ggcgcggtcc tcgtggtcaa ggcacagatt cacgcgggcg ggcgcggcaa gggccaggta
     3301 cttgaccgca caaaccaaaa taaacttgtg ctggacggtg gcgtcaaggt tgtgaaaagc
     3361 gcagaggaag ccaaggctat cgcctcccaa atgcttggca acacgctggt cacccatcaa
     3421 acaggtccga gcggcaagac ggtcaaaaca gtactggttg agacggcctg ccacatcaaa
     3481 aaagaatatt atttgggatt ggtgctcgac agaacgaaat cgcgcatcac gtttctggcc
     3541 tccgcagagg gtggcatgga catt
//
LOCUS       DATDUN010000175         1370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_342477, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000175 DATDUN010000000
VERSION     DATDUN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342477"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VJC18_02350"
     CDS             complement(<1..1119)
                     /locus_tag="VJC18_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLD44251.1"
                     /translation="MNTNEKTHVLVIDDEKPVCITCQRILEEEGFDVTFVLSGKDGIE
                     KAIAGNFQVILLDLKMPGLSGMETMAHIQQERPDIEIIIITGYATIQTSIEAIKEGAF
                     DYIPKPFTPDELLNSVRKALHNRQLKNNPTYSDQDVQSAMQASRVVGRSQAMQNVFQQ
                     ILKVALTDFTVMVYGESGTGKELIAKAIGQHSQRATKPFVGVDVSAIAPTIVESELFG
                     HVRGAFTGATSNRTGYFSTANGGTLFLDEISNISLELQGKLLRALETKHIQPVGSDKP
                     CQIDIRLITATNKDLSLLVKENKFREDLYYRINVIPITIPSLRERAEDIPLLATHFLN
                     NAKSKTQSEIKGFTTAAMAKMLAYPWPGNVRELKNVIER"
     gene            complement(1116..>1370)
                     /locus_tag="VJC18_02355"
     CDS             complement(1116..>1370)
                     /locus_tag="VJC18_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019282208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLD44252.1"
                     /translation="SGGFVQIVIKDTGKGIPREYRNRVFEPFFTTKGSRKGTGLGLSV
                     SLGIIQRHKGTIQLDSEENQGTTMIVIIPRIQPPTEEVLV"
BASE COUNT          295 a          311 c          384 g          380 t
ORIGIN      
        1 tcgttcaatc acatttttga gttcacgaac attgccaggc caagggtagg ccagcatctt
       61 tgccatggcc gccgtggtga atcccttgat ttcagattgt gttttgcttt tggcattgtt
      121 cagaaaatgt gtggccagca aagggatgtc ctcagcccgt tcgcgcaaag aagggatggt
      181 gatggggatg acgttgatgc gataatagag gtcttccctg aacttgtttt ccttgacgag
      241 gaggctcaag tccttgttgg tggcggtgat gagacggatg tctatctggc aaggtttgtc
      301 actgccaacg ggttggatgt gtttggtctc caaagcacgc aataatttcc cctgcagttc
      361 aaggctgatg ttcgaaattt catccaaaaa cagtgtgcct ccgttggctg tgctgaaata
      421 gccagtccgg tttgaggtgg cgccagtgaa ggctccacgg acgtgcccga agagttcgga
      481 ttcgacgatc gtgggagcga tggccgaaac atcaacaccc acaaaaggtt ttgtggcgcg
      541 ttgagaatgt tgcccaatgg ccttggcgat gagttctttg ccggtacctg attcgccata
      601 aaccataaca gtgaagtccg tgagggcgac tttgaggatt tgttgaaaga cgttttgcat
      661 ggcctgcgac cgccccacaa cgcgagacgc ctgcatggcc gattgtacat cctgatccga
      721 atacgtggga ttgtttttca attgacgatt gtgcagggct tttctgacgg agttcaatag
      781 ctcatctgga gtgaagggtt tggggatata atcaaaggca ccttccttga tggcctcgat
      841 gctggtctgg atcgtggcgt acccggtgat gatgatgatt tcgatatctg gacgctcctg
      901 ttgaatgtgt gccatggtct ccatgccgga caatcccggc attttcaaat cgagcaggat
      961 gacctgaaaa tttcctgcga tggccttttc gataccgtct tttccggaca ggacaaaggt
     1021 gacgtcaaat ccttcctcct caagaatgcg ctgacaggtg atgcacacgg gtttttcgtc
     1081 atcgatgacg agcacgtgtg ttttttcgtt ggtgttcata cgaggacctc ctcagtgggt
     1141 ggttggattc tgggaatgat gacgatcatg gttgtgccct gattttcttc gctgtccaat
     1201 tgaatggttc ccttgtgtct ttggatgatg ccgagagaaa cagacaatcc cagaccggtt
     1261 cccttacggg aacccttagt cgtgaagaag ggttcaaata cgcggttgcg gtattccctg
     1321 ggaatgccct tgcccgtgtc tttgatgacg atctgaacaa agccaccgct
//
LOCUS       DATDUN010000176         1143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_9169, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000176 DATDUN010000000
VERSION     DATDUN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9169"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1143)
                     /locus_tag="VJC18_02360"
     CDS             complement(<1..>1143)
                     /locus_tag="VJC18_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase GatDE subunit E"
                     /protein_id="HLD44253.1"
                     /translation="LIQLSIEEDACREVSDVGHWRTYMTDRLGMPLIEVVTHPDMKTP
                     REAAEAGQMIRFMTRSTGKVRTGNGAGREDVNVSVTGGQRVEIKGVPSIALIPKLTHN
                     EAYRQRALLLIKEELNRRVVQPKDWRVTHTVFSEVDLKLRFPRLALAVDGLNQLNRGN
                     VVVVNLPQFKGLLSFFTQLGRAFSDELADRLKVIACLEKPNLLTSEDISGVVTQEDFV
                     PLCASLPAGPEDALIIFLSPTDDIKTAVETIEERCRLAFAGVPNETRKPKRDGTTLFE
                     RVLPGPDRMYPDTDSAPIPISVQHVATLMAGLPRAVSAQMTQMNEWHVPTDTHEYLLR
                     HNLLPLFRRIVDEFSVSPVFVAALLGHTLKNIQGKMKPSAEFDYKK"
BASE COUNT          255 a          318 c          316 g          254 t
ORIGIN      
        1 cttttttgta atcaaactcc gctgacggct tcatcttgcc ctgaatgttc ttgagggtgt
       61 gccccaaaag ggccgccaca aaaacgggag agacagaaaa ctcatccaca atccttctga
      121 aaagcggtaa gagattatgg cgtaacagat attcatgagt gtccgtcggg acatgccact
      181 cgttcatctg tgtcatttgg gccgacacag cacgcgggag ccctgccatc agcgtcgcca
      241 cgtgttggac actgatgggg atgggggcag agtcggtgtc ggggtacatg cgatcggggc
      301 cgggaaggac acgctcaaac agggtggtcc catccctctt gggtttgcgg gtttcgttgg
      361 gcacccccgc aaaagccagt cggcaacgct cttcaatcgt ttcgacggcg gttttgatgt
      421 cgtccgtggg ggacaggaaa ataataaggg catcttctgg acccgctggc aacgaagcac
      481 acagaggcac aaaatcttct tgagtcacca cgccagaaat gtcttcgctc gtgagcagat
      541 ttggtttttc caaacacgcg atcactttga ggcgatcggc aagctcatcg gaaaaagcac
      601 ggcccagttg tgtgaagaag gacagcaagc ccttgaactg cgggaggttg accacgacca
      661 cattgccacg gttgagttgg ttcaggccgt caacagccaa agccaaccgt ggaaagcgca
      721 gtttgaggtc cacctctgaa aaaaccgtat gcgtgaccct ccaatcttta ggctggacaa
      781 cacgtctgtt gagttcctct ttgatgagga gcaaggccct ctgtcggtag gcttcattgt
      841 gcgtgagttt gggaatgagc gcaatgctgg gcacaccctt gatttccacc cgttgcccac
      901 cagtcacact cacgttcacg tcttcgcgcc ctgccccatt gcctgtgcgc accttgccgg
      961 tggagcgcgt catgaaccgg atcatctgtc ccgcctcagc ggcctcgcgc ggcgttttca
     1021 tatcgggatg cgtcaccacc tcaatgagtg gcatccccag acgatccgtc atgtaggtgc
     1081 gccaatgtcc aacgtcagaa acctcgcggc aggcatcttc ctcaatgctg agctgaatga
     1141 gtt
//
LOCUS       DATDUN010000177         3418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_298910, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000177 DATDUN010000000
VERSION     DATDUN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298910"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VJC18_02365"
     CDS             <1..362
                     /locus_tag="VJC18_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HLD44254.1"
                     /translation="DVVLVDTAGRLQTKTNLMNELKKMVRMTEKELGRDPDQIFLVVD
                     AITGQNGFSQARVFLEAIPLTGIILTKYDASAKGGIIISIVEQTGLPIRYVGLGEGMN
                     DLKPFNANEFVEKLFAA"
     gene            376..2094
                     /locus_tag="VJC18_02370"
     CDS             376..2094
                     /locus_tag="VJC18_02370"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLD44255.1"
                     /translation="MKIAINLDLRNPEQKYIKVTYEISGLTQKDLVVTFPTWTPGSYL
                     IREYQGKVEDLVLTDSHGKMVRQQKTSKHQWQFEHIKTKSVTLTYRVYANTLSVRDAY
                     ADFELVYITPCAVLCFLNNDLRLRPTLTITQPKGWSLALAKSASKGIYHFGDFEELFD
                     TPVLSAKNLIIKNFTLKNNHYRVAYWGICHRDLNEVTSDLKKIVASENRIFHDCPSKN
                     YLFQILFTKDAPAGGLEHRDSSTNLIDGSQLINQDKYLPFLSLLAHEYFHLWNVKRIR
                     PADFDRLDYTQERYTRELWMAEGITRYYDDHTLQRAKIYDPQKYLAVLGENISRMENN
                     KACRVNSLSDASFDAWIRWYRPNENSANRTISYYLKGGLVAMLLDFWIIKQSKGKFCL
                     DQVMQQLYKNWQNHPDQGVTRQDFFLTVQKFAKTSPKGFVRNYLDGTKAIAWSKEFAP
                     FGIKVLKKQDPKKNYLGISLKAQGDRIYIEQIIEDSPAFLSALQAGDEVLAINHECVR
                     SVADLDRFLSQKVLHFILGRRGVVVETTISLRPKSSPLFILEINKRLSSMQKRHLNKF
                     LRDNSI"
     gene            2105..>3418
                     /gene="asnS"
                     /locus_tag="VJC18_02375"
     CDS             2105..>3418
                     /gene="asnS"
                     /locus_tag="VJC18_02375"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006456769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HLD44256.1"
                     /translation="MTHQSIKEILKQSPIAKTVTIKGWLRTARHSKGLSFLEVNDGSC
                     FSSLQAIADDKLANYDSEVSRLLTGCAVAVTGLVVESPGKGQSIELQAQNIEVIGSSA
                     DDYPLQKKRHTFEYLRTIAHLRPRTNTIGAVLRVRNAAARAIHNFFQERDFFYLHTPI
                     ITASDCEGAGQMFRVSCLDAKTPPLKEDGKVDFSQDFFGRESYLTVSGQLEGEIGALA
                     LSKVYTFGPTFRAENSNTSRHLAEFWMVEPEVAFCDIQGDMDLAEEFLKYVFKSCLND
                     CPDDFAFFNERIDPTILETLKHIIESPFERITYTQAVDILLASGKAFEFPVSWGIDLQ
                     SEHERYLTEDHFKKPVMVTDYPKNIKAFYMYLNDDDKTVRGLDVLVPKVGEIIGGAQR
                     EHRLDVLQRRLVECGLSEKDYWWYLDLRKYGSVPHAGFGLGFERIV"
BASE COUNT          921 a          902 c          800 g          795 t
ORIGIN      
        1 ttgacgtggt tttggtggac acggcaggca gactgcagac caaaaccaat ttgatgaatg
       61 agctcaaaaa aatggtgcgc atgacagaaa aagagttggg tcgggatcca gatcaaattt
      121 tcctggtggt cgacgccatc accggccaaa acggtttttc tcaagcacgc gtctttctgg
      181 aggccatccc gctcacaggc attatcctca caaaatatga cgcctccgcc aagggcggga
      241 tcatcatctc cattgtggag cagacaggcc ttcccattcg ctacgtaggg ctgggagaag
      301 ggatgaacga tcttaagccg tttaacgcca acgagtttgt tgaaaaattg tttgcggcat
      361 gaaagaggag ctcacatgaa aatcgccatc aatctcgacc tgagaaaccc cgaacaaaaa
      421 tacattaaag tgacctacga aatttccggt ctcacacaaa aagatctggt ggtgaccttc
      481 cccacctgga cgcctggctc ctacctcatc agagagtatc agggaaaagt ggaagacctg
      541 gtcttgaccg actctcacgg caagatggtg cgccaacaaa aaacaagcaa acaccagtgg
      601 caatttgaac acatcaaaac aaagtcagtc acactcacct accgtgtgta cgccaacacg
      661 ctgagtgtca gggatgccta tgctgacttt gagttggtct acatcacgcc ctgcgcagta
      721 ctgtgctttc ttaacaacga tctgaggctc aggcccacac tgaccatcac gcaacccaag
      781 ggttggtcac tggccctggc caaatccgcc tcaaaaggca tctatcactt tggagatttt
      841 gaggaacttt tcgacacccc tgttttgtcg gccaaaaatc tcatcatcaa aaacttcacg
      901 ctcaaaaaca accactaccg cgtggcctac tggggcattt gtcatcgtga tctcaacgag
      961 gtcaccagcg atcttaaaaa aatcgtcgcg agtgaaaaca ggatttttca cgattgcccc
     1021 tcaaaaaact atttgttcca aatccttttc accaaagacg ccccggcggg cggactggaa
     1081 caccgcgaca gctcaaccaa cctcatcgat ggctcccagc tcatcaatca agacaaatac
     1141 ctgcctttcc tttctctgct ggcacacgaa tattttcact tgtggaatgt caagcgcatc
     1201 cgtcctgcag attttgacag gctggactac acccaagaaa gatacaccag ggaattgtgg
     1261 atggcggagg gcatcactcg ctactacgat gaccacaccc tgcaaagagc caaaatatac
     1321 gatccccaaa aatacctcgc cgtgcttggc gagaatattt cccgaatgga aaacaacaag
     1381 gcgtgtcggg tcaattcgct gtcagacgcc agctttgatg cctggatccg atggtatcgc
     1441 cccaacgaaa actcagccaa ccgaacgatc agctactacc tcaagggggg gctggtagcc
     1501 atgttgcttg atttctggat catcaaacaa tcaaaaggaa agttctgttt ggatcaggtc
     1561 atgcaacaac tctacaaaaa ctggcaaaac catcccgacc aaggcgtcac ccgtcaggac
     1621 ttttttttga ctgtgcaaaa atttgcgaag acctctccaa agggatttgt tcgcaattat
     1681 ctggacggga ccaaggccat cgcatggtca aaagaatttg ccccctttgg catcaaggtg
     1741 ctcaaaaagc aggatcccaa aaaaaattac ttgggcattt ctctcaaggc acagggcgac
     1801 cgcatctata tagaacagat catcgaggac agtcccgcct ttttgtctgc actgcaggcc
     1861 ggggatgaag ttctggccat caaccatgaa tgtgttcgct ctgtggccga tcttgaccgc
     1921 ttcttgtcac aaaaagtcct gcatttcatt ttgggccgaa ggggcgtggt ggttgaaacc
     1981 acaatctctt tgcgaccaaa atcaagcccc ctttttatac tggagataaa caagcggtta
     2041 tcgtccatgc aaaaacggca cctcaataaa ttcctcagag ataattcaat ataaggagtt
     2101 ggcaatgact catcaaagca taaaagaaat cctgaaacaa tcccccatcg ccaaaacagt
     2161 caccatcaag ggttggctcc gtactgcccg tcattccaag gggctgtctt ttctggaggt
     2221 caacgatggc agttgttttt cgagcctgca agccattgcc gatgacaaac tcgccaatta
     2281 tgacagcgag gtttcccgtc tgctgacggg ttgcgcagta gccgtcaccg gtctggtcgt
     2341 ggaatccccc ggcaaaggcc aaagcatcga gcttcaagcc cagaatatcg aggtcatcgg
     2401 gtcatcagcc gacgattacc ctctgcaaaa aaagaggcac acctttgaat acctgcgcac
     2461 catcgcccat ctacggcccc gcaccaacac cattggcgcg gttttgcggg tccgtaatgc
     2521 cgccgcgagg gccattcaca acttttttca ggaaagagac ttcttttatc tgcacacccc
     2581 tatcatcacg gcctcagact gtgaaggtgc cgggcaaatg ttcagggttt cgtgtttgga
     2641 tgccaaaacc cctcccctaa aagaagatgg caaagtggat tttagccagg atttttttgg
     2701 ccgcgaatcg tatctgaccg tttccggaca actcgaggga gagattggtg cgctggccct
     2761 gtcaaaggtc tacacctttg gccccacctt cagggcagaa aattcaaaca ccagccgtca
     2821 tctggccgag ttctggatgg tggaacccga ggtcgctttt tgcgatattc agggcgatat
     2881 ggatttggca gaggaatttc tcaagtatgt ttttaagtcc tgtctgaacg actgtcccga
     2941 tgattttgcc ttttttaatg agcgcataga tcccaccatt ttggaaacct tgaaacacat
     3001 catcgaatcg ccctttgagc gcatcacgta cacacaggca gtcgacattc tgctcgccag
     3061 cggcaaggcg tttgaatttc ccgtttcttg gggcattgac ctgcaaagcg agcatgagcg
     3121 ttatttgacc gaagatcatt tcaaaaaacc cgtcatggtg actgattatc ccaaaaacat
     3181 aaaagccttt tacatgtatt tgaacgacga cgacaaaacc gtaaggggct tggacgtgct
     3241 ggttcccaag gtgggcgaga tcatcggcgg cgcgcaacgc gaacaccgtt tggacgtgct
     3301 ccaaaggcgc ctggtggaat gtggtttgag cgaaaaagat tattggtggt atctcgatct
     3361 gcgcaaatac ggttccgtcc ctcacgccgg gtttgggctg ggttttgagc gcattgtg
//
LOCUS       DATDUN010000178         1631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_423983, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000178 DATDUN010000000
VERSION     DATDUN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423983"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VJC18_02380"
     CDS             <1..558
                     /locus_tag="VJC18_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLD44257.1"
                     /translation="GGSPNGLIHDLMTQVEQMDFKNPNVLKFEKYFETKIRNLAIEGI
                     DDETKLELDRSLDELLEFSDRWTRFRQLGLGNTLGTFRNATALGKLNPDVSEGGLTLS
                     TVHTMKGLEKDIVFLMGFCEGTFPDYRANTTPKMNEERNTAFVAITRARRWLYVSYPQ
                     SRMMPWGSQRSQQPSRFISEMGLAQ"
     gene            758..1135
                     /locus_tag="VJC18_02385"
     CDS             758..1135
                     /locus_tag="VJC18_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44258.1"
                     /translation="MDTEEEKQNVAKLAKVMALMCVRNTGLENIHAGRSPISEIGNYS
                     DVFVHDAKGQKIPWADVSHINDEEMKALMKEIVNHIYTFLLHIDDPDFQAWMERWATV
                     SNKWDDPELEKSFLVTLSDSNSR"
     gene            complement(1449..>1631)
                     /locus_tag="VJC18_02390"
     CDS             complement(1449..>1631)
                     /locus_tag="VJC18_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44259.1"
                     /translation="DTIEAVGVKPDGGRGANPPFQTKNTRREHVRSPLPWAVMACRKG
                     EKMRSVKLKKSTKTIT"
BASE COUNT          417 a          400 c          400 g          414 t
ORIGIN      
        1 ggcggttccc ctaacggctt gattcacgat cttatgacgc aagttgaaca aatggatttt
       61 aaaaatccta acgtcctgaa gttcgagaag tactttgaaa cgaaaattcg aaacttggcg
      121 atcgagggaa ttgacgatga aacgaaactg gaattagatc gcagccttga tgagttgctg
      181 gagttctctg atcggtggac acggtttcga caacttggcc ttggcaatac gcttggcacc
      241 tttaggaatg ccactgcttt ggggaaattg aatccagatg tgtccgaagg cggattaact
      301 ctaagcacag ttcataccat gaagggactt gagaaggata tcgtgttttt gatgggtttt
      361 tgtgaaggta cgtttcccga ttaccgagca aatacaacgc caaagatgaa cgaagaaagg
      421 aatactgcct ttgttgccat cactcgggcc cgtcggtggc tttatgtttc ctaccctcaa
      481 tctcgtatga tgccgtgggg ttcgcagcga tcacagcaac catcccgatt catttcggaa
      541 atggggcttg ctcaatgaac gtcaattgcc cgggacatca agtaccatta gctgattaat
      601 ggcgatgtga catgcagaag ttttgcgctg tgcacgaatg gcaggtttcc aaaaggtcat
      661 ctgtgcatgt aggatgaccg ctttgggccg attgtaaact ccacattgat ttcgccaacc
      721 tatggcctat ccagacatcc cttgatagca taacagcatg gatacggaag aagagaaaca
      781 aaacgtcgca aaactggcca aagtcatggc cttgatgtgc gtgcgcaaca ccggcttgga
      841 aaacatccat gccgggcgct cccccatcag cgagatcggc aactactctg acgtttttgt
      901 ccatgacgcc aagggacaga aaataccctg ggccgacgtg tcccatatca acgacgaaga
      961 aatgaaagcg ttgatgaagg aaatcgtcaa ccacatctat acctttctcc tgcacatcga
     1021 cgatccggac ttccaggcct ggatggaacg ttgggcgacc gtgtccaaca agtgggacga
     1081 tccggaattg gaaaagtcgt tccttgtgac gttgtccgac tcgaacagtc gctgatcagt
     1141 ctccccgcca aaccttatcc acacacctgt ggaaaccccg cgcgcaccga ttcataatcc
     1201 agtgcaaaac ccgtttttgc cttcgtatga tcgggcgcag atgttcgcag cattctcaac
     1261 aacactttgt tttcgcgtcg ccaatccggc cctaatagct gaatcaaact tataaaaact
     1321 ttctgatttt ggggcggccg atttttgtcc agcagctctc tggccggacg gcgcggcgta
     1381 aaaaaaaatc gcgatagctg gaattgtccg gtttgatttg gcgatatcgc aagatgcctg
     1441 aagttgcatt aagttattgt tttagttgat ttttttagtt tgacagatcg cattttctcg
     1501 ccttttctgc aggccataac cgcccaagga aggggagagc gaacatgctc acgtctcgta
     1561 ttttttgtct gaaaaggggg attcgcccct cgcccgccat ccggcttcac gccaaccgct
     1621 tcgatggtgt c
//
LOCUS       DATDUN010000179         1563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_133686, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000179 DATDUN010000000
VERSION     DATDUN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133686"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VJC18_02395"
     CDS             <1..1115
                     /locus_tag="VJC18_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLD44260.1"
                     /translation="LNNNIVTQLSALSQKATQKLSLLTPLSAEEKKGIHQLARVSQVG
                     ASTRIENAILTDAEVLWIDTTLVRDARVGVFQKYEYQIKDKLSRDRERSIEEVAGCRN
                     MHFLIYEQARDFIPLTEITLRGLHAELLQFYPKAKHYLGQYKNVTNSVVEINHKTKDQ
                     RIVFKTADPGPITQAAMSDLVAWYNNTASQFPWTIAVACEFVFRFLAIHPFQDGNGRV
                     GRGLFLLSLLQSSDSVLAEVGRYVAIDRQIEKHKEEYYLVLQRCSGGKFLSDPKKYQF
                     EIFLKFMLKVMDEALDDVDYYLNRLKTTQALSDTARLVLKCINDFPEKKLQTNDIIQQ
                     TKLPRRTVVYSLNALIKSGLIQRNGQGPATRYQVVF"
     gene            1112..1282
                     /locus_tag="VJC18_02400"
     CDS             1112..1282
                     /locus_tag="VJC18_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44261.1"
                     /translation="MINLYQSSAFHHNVSGIVTSNITATDITMINMKTQFLSPSTSAS
                     GYRDRTNLIYGE"
BASE COUNT          456 a          352 c          336 g          419 t
ORIGIN      
        1 aattaaataa caatattgtc acgcaattat cagcacttag tcagaaagct actcaaaagc
       61 tctctttact cacaccgcta tcagccgaag aaaaaaaagg cattcaccaa ctggcccgag
      121 tcagtcaggt tggtgcctcc acacgaatag agaacgccat tcttaccgat gctgaagttt
      181 tatggattga taccacattg gttcgagacg ccagagtggg tgtttttcaa aaatacgaat
      241 accaaatcaa agacaagctc tccagggatc gtgaacgtag cattgaagaa gtggcaggtt
      301 gtcgcaacat gcatttttta atctatgaac aggcacgtga ttttatcccg ctgaccgaaa
      361 tcacgctgag gggtttgcat gcggagcttc ttcagtttta tcccaaggct aaacattatt
      421 tgggccaata caaaaacgtg accaacagcg ttgttgagat caatcacaaa acaaaagacc
      481 aacgcattgt ttttaaaacg gcagatccag gtcccatcac acaagcggcc atgagtgatt
      541 tggtggcctg gtacaacaat acggcttcgc aattcccgtg gaccatcgct gtggcatgcg
      601 agtttgtatt tagatttttg gcgattcatc cttttcaaga tggtaacggt cgtgttggtc
      661 gaggcctctt tttattatca cttcttcaat cttccgattc tgttctggca gaggtgggac
      721 ggtatgttgc gattgatcga cagattgaga aacacaaaga agaatattat cttgttttgc
      781 aacgttgttc gggtggcaaa tttttgtcag atccaaaaaa atatcagttt gagatctttc
      841 tcaaattcat gctcaaggtg atggacgaag ccctcgatga tgtggactat tatttgaatc
      901 gccttaaaac aactcaagct ctttccgata cagccaggct ggttttgaag tgcatcaatg
      961 attttccaga aaaaaaattg cagacaaacg acatcattca gcaaacgaaa ttgcctcgcc
     1021 gaactgttgt ttactcgctc aacgcgttga tcaagtcggg attgattcag agaaacggcc
     1081 aggggccagc cacgcgatat caagttgtgt tttgatcaat ctgtaccaaa gttctgcctt
     1141 tcatcacaat gtgtcgggaa ttgtgaccag caacatcacg gcaacagata tcacaatgat
     1201 caacatgaaa acacaatttc tctcacccag cacgtctgct tcagggtatc gggacaggac
     1261 gaatctgatt tatggtgaat aagctgctgt ctattgacgc ttggcgtgca atttttttct
     1321 ttgtaggggg gccggtcgcg accggccccc actgctttcc gtaaaaaaca tgaacacacg
     1381 aacgcgcctg ccccacaaag ctcgaagagc gtagtggggt aaacacgttc ttcacctttg
     1441 acctttgacc tttgaccttt gagctaatat acccgcccca tgttaccttt caaagaatac
     1501 gagccaaaat ggcaaaaaaa atgggcagaa aataaaattt ttcgcgctga gaatcattca
     1561 tca
//
LOCUS       DATDUN010000180         1123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_49985, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000180 DATDUN010000000
VERSION     DATDUN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49985"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1123
                     /locus_tag="VJC18_02405"
     CDS             <1..>1123
                     /locus_tag="VJC18_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44262.1"
                     /translation="WDDNARSVDEAKEHLQFSIDYAHERFPGKKFYVGEIGWPSQGRM
                     RWKAEPSPVNEARYLRAVIPYLEKAGLHYNIIEAFDQPWKRNNEGCVGGYWGVYDEGR
                     RDKNIFSGPLSNESKWLFLFFCSVLLSITLLTPVRWSAVQNSFVKSLLLFFLAALASF
                     VLVAQGRAFYLSAFNLLAMSKAVFFLVFGLLGTLLMMRNVVHGQPRPSLRIPHQRDEA
                     IQNKVSEQWPFHSVALRWAHGKFAQGTRNGFIIFEKLTVFLFITISLSLSFAGRSSSF
                     LSLAFLFPVLFVLNRAWSQRHIPANTTTSMAEGILALILFVLILLNEGVINGQANLWM
                     LLCVLTAMARWGVFSKTRLGELLRQTPWRTGVKYALGLA"
BASE COUNT          239 a          276 c          282 g          326 t
ORIGIN      
        1 attgggatga caacgcccga tctgtagatg aagccaaaga acatcttcag ttttccattg
       61 attatgcaca cgaacgattt cccggcaaaa aattttacgt aggtgagatt ggctggccca
      121 gtcagggccg catgcgctgg aaggccgagc ccagccctgt caatgaagcc agatacctgc
      181 gagccgtgat cccttatttg gaaaaagcag gtcttcatta caacatcatc gaggccttcg
      241 accagccctg gaagcgcaat aacgagggtt gcgtgggagg ctactggggt gtttatgatg
      301 aaggacggcg cgataaaaat attttttctg gtcccctctc caacgaatca aaatggctgt
      361 tccttttttt ctgctcggta ttattatcca tcacattgct gactccggtg cgctggtcag
      421 ccgtgcaaaa cagttttgta aaatcccttc tgctgttttt tctagccgca ctcgcctcat
      481 ttgttctggt ggcacagggc cgggcttttt acttgagtgc ctttaatttg ttggccatga
      541 gcaaggccgt tttctttctt gtctttggat tgttgggaac ccttttgatg atgcgcaacg
      601 ttgttcacgg tcagcccagg ccgtcattgc gaatccctca tcagagggat gaagcaatcc
      661 agaataaagt gtcagagcaa tggcccttcc acagcgtcgc ccttcgatgg gctcacggca
      721 agtttgctca gggcacacgc aatggcttca ttatttttga aaaactgacg gtgttcctct
      781 tcattaccat cagtctgagt ctctcttttg ctgggcgcag ttccagtttt ttgagtctgg
      841 cctttttgtt tcccgtatta tttgttctca acagggcttg gtcacaacgc cacatacccg
      901 caaatacgac cacctcaatg gccgaaggga ttttggcact catattgttt gtccttattt
      961 tactcaacga aggcgtgatc aacgggcagg ccaatttgtg gatgcttctt tgcgtgctca
     1021 cggcgatggc acgatggggc gttttttcca agacacgctt gggggagctt ttgcgccaga
     1081 ccccttggcg cacgggagtg aaatatgctt tggggcttgc gtt
//
LOCUS       DATDUN010000181         3269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_133768, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000181 DATDUN010000000
VERSION     DATDUN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133768"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VJC18_02410"
     CDS             <1..799
                     /locus_tag="VJC18_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HLD44263.1"
                     /translation="RLTARIQETFGINPHCACQYDALKDLPIEALHGVFFDDQTVPNS
                     LAQMGKSVLPIRIVDRARGSSVIVSPLGHVITAHHVFYDPETGKPRENIKLKYNDEME
                     FTVSEENLRYVSREGDLVILQIPELAQQKDLCFAQVRQTPISEGSELVDVGFPQTAME
                     NIMKRFYTVGAVIAPHENPSENEQEDFAGLRKMGYNFERFIMSNCVNVHGASGGAIFD
                     SDGRVVGINTAFADQAKIGLHSDLTSPGTDEKLRVVLQEIIATYHNG"
     gene            893..1252
                     /locus_tag="VJC18_02415"
     CDS             893..1252
                     /locus_tag="VJC18_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLD44264.1"
                     /translation="MTDKDNLKLLEIGRKVRGWVASDERRRRWPEEIKTDVVALIRQG
                     FSMSDLSRTTGIYCATISKWRFQKKKPGLFSELALVLHPRIVNDATSEISLETARGHF
                     VSLPLSALSALFQAGVL"
     gene            1252..1605
                     /gene="tnpB"
                     /locus_tag="VJC18_02420"
     CDS             1252..1605
                     /gene="tnpB"
                     /locus_tag="VJC18_02420"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HLD44265.1"
                     /translation="MVLFNHRYLKVFVCSRIVDMRGGFESLGRIVSAEMGGDLLDGHV
                     FLFFGRNRRRAKALLFDGTGLVLIHKRLETGRFMRPDEFENSGSITMAELGLILDGTS
                     FKLPLATRQFFLAEK"
     gene            1674..3239
                     /locus_tag="VJC18_02425"
     CDS             1674..3239
                     /locus_tag="VJC18_02425"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HLD44266.1"
                     /translation="MLSNREVILSENHPEVLKGLALFILENNEKLAKENAKLRKLEAE
                     SKLRELELDYQRTILRDLVFGHGVEKSRASSGRNRRIKPNEQLLLHAQSLAPAPTEEE
                     RKNLERSVVSHEMTGAELVTVAKEMGLDRQEQSAFSEIKGLTDDAVEITVIERRYISE
                     LHKLKKYRYHPAPDENLEKEVIVTAERTDKLFSGAGYSIDFALSVVRDKYEYHKPLER
                     QTREMQRLGLNVGTKTLYNMAEAVSVHMEPTVSLVLQDIFKAGLAVECDETPWFIQSN
                     HDDDGQMWTASNQAGTYYRFEPTRSGKVIAEILKDYAGPVMADGYGGYNRLTKDKKID
                     LAGCWAHARRKFIELEDVYPEDVGWVLDRIGELSSIEYEAKNYEELQKLRHEKSRPLA
                     EKIRGYLVEMKTRHLPESALIKKAVNYTLKRWDCLTKFLDDARIPLTNNGAERAMRHA
                     VMGRKNFNGSQTINGADTASVLYTIIESCKKVEVDPTTFMKEIILRNNRDEPSISPLQ
                     YAKQIRTPPAENQ"
BASE COUNT          960 a          652 c          864 g          793 t
ORIGIN      
        1 acgcttgacg gcacgaattc aggaaacctt cggaatcaat cctcattgtg cctgtcaata
       61 tgacgcactg aaggatcttc cgatcgaggc gctgcacggc gttttttttg atgaccagac
      121 tgtgccgaac tcattggcac aaatgggcaa gtctgttttg ccaatccgca ttgttgatcg
      181 cgctagaggt tcttctgtga tcgtttcgcc gttggggcac gtgataacgg ctcatcatgt
      241 tttttacgat cctgagactg ggaaaccgcg ggagaacatc aaactcaaat acaacgacga
      301 aatggaattc actgtttcgg aagaaaactt gagatacgta agccgggaag gcgatctagt
      361 gattttgcag attcccgagc tggctcaaca gaaggatttg tgttttgcac aagtcaggca
      421 aacacccatc tcagaaggaa gcgaactcgt tgatgttggc tttcctcaaa ccgcaatgga
      481 aaatatcatg aaaaggtttt atacagttgg cgctgttatt gctcctcatg aaaacccgtc
      541 ggagaatgaa caagaagact ttgcaggttt acgaaaaatg ggatacaatt ttgaacgttt
      601 catcatgagc aactgtgtca atgttcatgg ggcgagtggg ggggcgattt ttgactcgga
      661 tggccgcgtg gttgggatca acacagcctt tgctgatcag gccaaaattg gattgcacag
      721 cgatctgacg tcacccggca cagacgaaaa actccgcgtt gtcttgcagg agatcattgc
      781 gacttatcac aatgggtgac tcctcagttt aaacacttcg taacccagtc ctaaaccctg
      841 tacttctcgg tttcaaaaat ttctgctaaa gcctccagtc aggaggtgat tcatgacaga
      901 taaagataat ttaaaattgt tagagattgg ccgcaaggtg cggggctggg ttgcttcaga
      961 cgagcgtcga aggcggtggc cagaggaaat aaaaacagat gttgtagcgc tcatcagaca
     1021 gggattttcg atgagcgatt tatcacgcac cacgggtatc tactgcgcga ccatcagtaa
     1081 atggcgtttt caaaagaaaa aaccgggcct gttttcagaa cttgctttgg tccttcaccc
     1141 ccgaattgtt aatgatgcaa cgtcagaaat ttctcttgaa acggcgcgtg gtcattttgt
     1201 cagcctgcct ttgtcagcat taagtgcgtt gtttcaagca ggagtgttgt aatggtttta
     1261 ttcaaccacc ggtatctgaa agtatttgtg tgttcgcgga tcgtggatat gcgtggcggc
     1321 ttcgagagtc tggggcgcat cgtgtccgca gagatgggtg gagatttgct ggatgggcat
     1381 gtgtttttat tttttggcag aaatcgacgg agggcaaagg cgttgttgtt tgacggaacg
     1441 gggctggtgc ttattcacaa gcggttggaa acgggacgat ttatgagacc cgatgaattt
     1501 gaaaactctg gatctataac gatggccgag ttgggtttga ttctggatgg aacaagtttt
     1561 aaattgccgc tggctacccg acagtttttt ctcgcagaaa aatgatgaaa aatatttttg
     1621 tttttgtgta aaaggtcttt gagaacggca aatgctgtga tagagtacat ttaatgttat
     1681 ccaacagaga agtcattctc agcgaaaatc atccagaagt cctaaaagga ctcgctctct
     1741 tcattcttga aaacaacgaa aaactggcaa aagagaatgc caaactcaga aaactcgaag
     1801 cagaaagtaa gctgagagaa cttgaactcg attaccagcg gacaattttg cgtgaccttg
     1861 tctttggaca tggcgtcgaa aagagtcgcg cctcatcagg gcgcaaccgc agaatcaagc
     1921 caaatgagca attgttattg cacgcccaat ccctggcacc cgcaccaaca gaagaagaac
     1981 gtaaaaatct tgaaagatct gtagtgagtc atgaaatgac gggagcagag ctggtgactg
     2041 tggcaaaaga gatggggcta gaccgtcagg agcaatccgc attcagtgaa atcaaggggc
     2101 ttaccgacga tgcagtcgaa atcaccgtga tagagcggcg ctacatcagc gagttgcata
     2161 aactaaaaaa gtatcgttat catccagcac ctgacgaaaa tttagaaaaa gaagtcatcg
     2221 ttacggcaga gcgcacagac aagcttttca gtggagcagg atattcgata gattttgccc
     2281 tttcagtggt aagagataaa tatgagtatc acaagcctct tgagcggcag acgcgtgaga
     2341 tgcagcggct tggacttaat gtggggacca agacgttgta taatatggcc gaggcggtga
     2401 gtgttcatat ggaacccacg gtgagtctgg tgttgcagga tatttttaaa gcgggtctcg
     2461 cagttgagtg tgatgaaaca ccgtggttta tccaaagcaa tcatgatgat gatgggcaga
     2521 tgtggacagc gtcgaatcaa gcggggactt attatcggtt tgaaccaaca cgaagtggca
     2581 aggtgatagc agagatactc aaagactatg cggggccggt gatggccgat ggttatggag
     2641 gatataacag actcacaaag gacaaaaaaa tagatttggc aggatgttgg gcgcatgcgc
     2701 ggcgcaaatt catagaactg gaagatgttt atccagaaga tgtggggtgg gtcctggacc
     2761 gaattggcga gctgagttca atagaatacg aggcaaaaaa ttatgaggag ctgcagaaac
     2821 tgcggcatga aaaatcgaga ccattagccg aaaaaatcag agggtatctg gttgaaatga
     2881 aaacacggca tttgccagag agcgctctga tcaaaaaagc ggtcaactac acactaaaac
     2941 ggtgggactg tctgacgaag tttttggatg atgcgcggat acctttgaca aacaatggcg
     3001 cagagagggc catgagacat gccgtgatgg ggcgaaaaaa ttttaatggg tcacagacaa
     3061 tcaatggtgc agataccgcg tcggtactct acacaatcat cgagagttgc aagaaggtag
     3121 aagttgatcc gacaacattc atgaaagaga taattctgag aaacaacaga gacgaaccct
     3181 caatctctcc actccaatac gccaaacaaa tcagaacacc gcctgccgaa aatcagtaaa
     3241 gtcaatgaca gggtttagga ctgggttac
//
LOCUS       DATDUN010000182         1284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_9481, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000182 DATDUN010000000
VERSION     DATDUN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9481"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VJC18_02430"
     CDS             <1..809
                     /locus_tag="VJC18_02430"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HLD44267.1"
                     /translation="RESSRPPQDIIDEINKLTERGVKEVTLLGQNVNSYGLKEKLGIS
                     FPALLEAIARQTSLKRLRFATSHPKDVSDELIAQFAVNPILAPQFHLPLQSGSDRILK
                     LMNRHYSQEQYLGLVAKLKKAVPHINFSTDLIVGFPSETDDDLQQTLEVMRQVGYTQT
                     YSFVYSPRPHTTAAHLPDDVPLQEKAQRLKTLQELDREVVSQLNQKDVGTVQSVLMEE
                     CRGQEPHPYLGRTPGHKVIHFSTTKKISPGNFVDVRVTRANPHCLFGETV"
     gene            887..>1284
                     /locus_tag="VJC18_02435"
     CDS             887..>1284
                     /locus_tag="VJC18_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HLD44268.1"
                     /translation="MSDLIKMNVSGLTIDPFTNMPIVILKDEEGTLALPIWIGLIEAS
                     AIATEIEKIALSRPMTHDLIKTLLEKLHASVQRIEITDLMDNTFFALIHCQSLNNQFS
                     LDCRPSDAIAVALRTKSDIYVDRKVIEKSR"
BASE COUNT          332 a          344 c          315 g          293 t
ORIGIN      
        1 gacgcgagtc gtcacgcccc ccgcaagaca tcatcgacga aatcaacaaa ctgaccgaga
       61 ggggagtgaa ggaagtcact ctgttgggcc agaacgtgaa ctcttacgga ctcaaggaaa
      121 agctgggcat ttcttttccc gcgctgttgg aggcgattgc caggcaaaca tccctcaaac
      181 gattgcgttt tgccaccagt caccccaaag atgtgagcga cgagctgatc gcgcaatttg
      241 cggtgaaccc catccttgcg ccccaatttc acctgcctct gcagtcaggc tccgatcgca
      301 tcctcaagct catgaacagg cactattcgc aggaacaata tttggggctc gttgccaaac
      361 tcaaaaaggc tgtcccgcac attaattttt ctacggactt gattgtgggg tttccttccg
      421 aaacggatga cgacttgcaa caaaccctcg aagtcatgcg tcaggtgggg tacacgcaga
      481 cctactcatt cgtgtattct cccagacccc acaccacagc ggcccatttg cccgatgatg
      541 tgcctttgca ggaaaaggcc cagcggctga aaacactgca ggagctggat cgggaggttg
      601 tgagccaact caatcaaaag gatgtgggca cggttcaatc ggtgctgatg gaagaatgcc
      661 ggggtcagga gccgcacccc tatttgggaa gaaccccggg acacaaggtg attcacttta
      721 gcaccaccaa aaaaatttcg ccgggtaatt ttgttgacgt gcgcgtgaca cgagccaacc
      781 cccattgcct ttttggggaa actgtttagt ctcttgtatt ttggaatttg atgatttatg
      841 tgattcggac tggcgaacca ccacgagatt tgagggagca cacgacatga gtgatttaat
      901 caaaatgaat gtatcaggac tcacaataga tccttttacg aacatgccca tcgtgatttt
      961 gaaggacgaa gagggcacat tggcactgcc tatctggatt ggcctcattg aggcctctgc
     1021 tatcgccacg gaaatcgaaa agatcgcgct ctcccggccg atgacccatg acctcatcaa
     1081 gacattgctc gaaaagttgc atgcctcggt ccaacgcatt gagatcaccg atctgatgga
     1141 caacactttt ttcgccctga ttcattgcca gagtctgaac aaccaatttt ctctcgattg
     1201 ccgcccgtcc gacgcgattg cggtggcgct caggaccaaa tccgatatct acgttgaccg
     1261 caaagtgatc gaaaaatcac gaaa
//
LOCUS       DATDUN010000183         1082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_50084, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000183 DATDUN010000000
VERSION     DATDUN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50084"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VJC18_02440"
     CDS             complement(<1..690)
                     /locus_tag="VJC18_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44269.1"
                     /translation="MNNKTFLVVVAAIIILGGAYYIRTSEDQAPVSISNPRNTAYQID
                     GQEYLLVNGTVEKETAPGSASKDTVRVFGEPVVGDLDADGDTDAAIFLVKNSGGSGTF
                     YYVALAMNENGTYRGTNALFLGDRIAPQTLEIHEGRAVANYAVRAAEESFAVSPSIGK
                     SLWIHFDQANFEIGEWVKDFEGEADPLRMSLGMKTWVWESALYNDGRKITPKRQDAFT
                     ITFKNDGSFSAT"
     gene            818..>1082
                     /locus_tag="VJC18_02445"
     CDS             818..>1082
                     /locus_tag="VJC18_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44270.1"
                     /translation="MNDHAILETLRTGDVLNVSTTNPANGEVDVLFSVVLREEIGPNG
                     TRLLYLSRLEHERPMAVPIRQNPYHWDSGVGTLVDRDGDPSLEL"
BASE COUNT          262 a          292 c          242 g          286 t
ORIGIN      
        1 tgtggcagag aacgagccat catttttgaa tgtgatcgta aatgcgtctt gtctctttgg
       61 ggtgatcttt ctgccatcat tatagagagc tgattcccaa acccacgttt tcatgccaag
      121 cgacatacgg agaggatcgg cttctccttc gaaatctttt acccattccc cgatctcaaa
      181 atttgcttga tcaaaatgga tccagagact ttttccgatc gacggcgaaa ccgcaaaact
      241 ttcctctgcg gcacgcacgg catagttcgc cactgcccgc ccctcatgga tctcaagcgt
      301 ttgcggtgcg atacgatcac caaggaacag tgcgttcgtg ccacgatacg tgccattctc
      361 gttcattgcc agtgcaacgt agtaaaacgt accgctcccg ccggaatttt tcaccaaaaa
      421 tatcgcggca tcagtatcgc cgtctgcatc aaggtctccc acgaccggtt caccaaaaac
      481 cctcactgtg tctttgcttg cagatcccgg ggcggtctct ttttcaacag tgccgttgac
      541 gagcaggtat tcctgaccat cgatctgata cgccgtgtta cgcggattag agatcgacac
      601 cggagcctga tcttcgcttg tgcgtatgta gtatgcaccg cccaaaataa tgatcgccgc
      661 cacaaccacg agaaatgttt tgttgttcat accgcatagt ttatcatgcg catgaatcat
      721 gccccacact agacaaaact gatatttttt gctataataa tactagatat attttggttt
      781 ggcttttgtt ccactacaac aatcccagga ggtgggtatg aacgaccatg ctattttgga
      841 aacactccgc acaggcgacg tgctcaacgt ctccacaacc aatcctgcaa acggcgaggt
      901 tgacgtactc ttctccgttg tgcttcgtga agagattgga ccaaatggaa cacgtctcct
      961 ctacttgtca cgccttgagc acgagaggcc aatggcggtt ccaatccgac agaatccata
     1021 tcattgggat tcgggagtgg gcacactggt tgatcgtgac ggagaccctt ctcttgaact
     1081 gg
//
LOCUS       DATDUN010000184         1125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_299279, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000184 DATDUN010000000
VERSION     DATDUN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299279"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VJC18_02450"
     CDS             <1..477
                     /locus_tag="VJC18_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HLD44271.1"
                     /translation="ILYLLLGSVDPIAWARLLVILAFVIVGLVILFFQSKKLNVLSLG
                     DEEAHHLGLNVNREKKIIFVITSLLVGASVSVCGLIGFIGLIVPHITRLLFGADNRRV
                     LPISALLGGLLLMTCDFVAGNLFSFDTLNTRLPVGAITALIGAPLFVWLLKRQIRT"
     gene            675..986
                     /locus_tag="VJC18_02455"
     CDS             675..986
                     /locus_tag="VJC18_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44272.1"
                     /translation="MKQNATTIKPVGQLMVLDEKTDVYHKTFTVKINEKNELVFEAYE
                     RGQALKDAYGEGEDERWLTVGPEFKDTVLLNLIKEQFKSDIELKAWLEKKGIPCKMVS
                     F"
BASE COUNT          271 a          236 c          307 g          311 t
ORIGIN      
        1 attctgtatc tattgttggg ttcggtagac cccattgcct gggcgagact cttggtcatt
       61 ctggcctttg ttattgtggg ccttgtcatt cttttttttc aaagcaaaaa actcaacgtt
      121 ctgtcgttgg gtgatgaaga agctcatcat ttgggcctca acgtgaatcg cgaaaagaaa
      181 atcatttttg tgatcacgtc cctgttggtg ggggccagcg tgtcggtttg cggattgatc
      241 ggttttatag gtttgattgt tcctcacatc acccgtctgc tttttggagc ggataatcgt
      301 cgcgtcctgc ccatcagcgc gttactaggc gggcttctgc tcatgacgtg cgattttgtg
      361 gccggcaatc ttttttcgtt cgataccctc aacacacgac tgcccgtggg ggctatcaca
      421 gcgttgatcg gcgccccttt gtttgtttgg ttgctaaaga ggcagattcg gacgtaggtg
      481 tgatggggag gctaatcaat tcctgtctgc aaacggaaac aagacagttt ttggacagga
      541 attctataag aagggagctg tttatgcatt caagaaagtg gtgctatacg gtttgtacgg
      601 tctgggtagt ttgcttgatc tgttctggga tctctttggc ccagacaaca ccaacaccaa
      661 caaaggatgg tcagatgaag caaaacgcaa caaccataaa accagttggt caactcatgg
      721 ttctcgatga gaaaacggat gtctatcaca aaacgtttac ggtgaagatc aacgagaaaa
      781 acgagttggt ttttgaggcc tatgagcgtg ggcaggcgct aaaagatgcc tatggtgagg
      841 gagaggacga acgttggctg accgtcggcc cggaatttaa agacacagtg ttgctcaacc
      901 tcattaagga gcagttcaaa tccgatattg aattgaaggc ctggcttgaa aaaaagggca
      961 ttccctgcaa aatggtgtcg ttctgattgt ggttacgtgc caataggtgc ctattcaaaa
     1021 acctgccggt aagaggtctt ttttgggtga cacggtgagc tgtttttgcg cgaataggcg
     1081 agcacgccgc tgggcgagct tgcgagccca gagcgccaaa aaaca
//
LOCUS       DATDUN010000185         1340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_215735, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000185 DATDUN010000000
VERSION     DATDUN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215735"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VJC18_02460"
     CDS             complement(<1..405)
                     /locus_tag="VJC18_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLD44273.1"
                     /translation="MKIKWSLLFSVAVILVVLFSSYFIVKETEQVVITRFGKPVRSAI
                     KEAGLYFKIPLIDKANYFAKTILEWDGVVEESKPIPTKDKKNIVVDTTARWRITDALT
                     FLQTVRSEEEAQSRLDDIIDSTIRDVISNHDLI"
     gene            complement(402..>1340)
                     /gene="hflK"
                     /locus_tag="VJC18_02465"
     CDS             complement(402..>1340)
                     /gene="hflK"
                     /locus_tag="VJC18_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HLD44274.1"
                     /translation="LKKLSLIPVIVFLLVLALPNMVYTVAPDEDAVVLRFGKFNRTVK
                     PGLHFNLPFGLETVSKVEVRKIKKMEFGFRTEKAGVQSTYRAGDYGPEKTMLTGDLNV
                     VDVEWIIQYQIDDAQKYLFKVRDVRKNLFDISQAVVREVVGDHTVAETLTEARDEISN
                     QSLEKMQEILDKYEMGIRLVALKLQDVFPPNEVKPSFDAVNAAVQDANQIANQAEKIR
                     KKRLQEERGSAEQVVKNAMAYKINLVNRSLGDAQRFLALYHEYQKAPEITKKRLYFDY
                     MTEAMHKADRFYVVDPEVKGIVPLLSITAQSGGGAQ"
BASE COUNT          330 a          353 c          296 g          361 t
ORIGIN      
        1 gatcagatcg tggttactga tcacatcgcg gatggtcgaa tcaatgatgt catccaaacg
       61 cgactgggct tcctcttccg atctgactgt ctgcaaaaat gtgagggcgt cagtgatgcg
      121 ccaacgcgcg gtcgtatcga ccacaatatt tttcttgtct ttggtgggga tgggtttact
      181 ttcctcaacc acaccatccc actccaaaat cgttttggcg aaatagttgg ccttgtcgat
      241 caaagggatc ttgaaataca agcctgcctc tttgatcgcg ctacgcacgg gtttgccaaa
      301 acgcgtgatc acgacctgtt ctgtctcctt gacaatgaaa tagctggaaa acaggaccac
      361 cagaataacg gcaacgctaa ataataaact ccatttgatc ttcattgagc acctccacca
      421 gattgggcag ttattgacaa taagggaaca atgcccttca cttcgggatc cactacgtaa
      481 aacctatctg ccttgtgcat ggcctccgtc atgtaatcga aataaagcct ctttttggtg
      541 atctccgggg ccttctgata ttcgtgatac agggccagaa acctctgggc atcgcccagc
      601 gagcgattta cgagattgat cttgtacgcc atggcattct tgaccacctg ctcggcactc
      661 cccctttctt cctgcagacg ttttttgcgg atcttttcgg cctggttggc gatctggttg
      721 gcatcctgta cagccgcgtt gacagcatcg aacgagggtt tgacctcatt gggggggaaa
      781 acatcctgca attttagggc cactaggcga atgcccattt cgtacttatc gagaatctcc
      841 tgcatttttt ccagagattg attgctgatt tcatcccgcg cctcggtgag ggtctcggca
      901 acggtgtggt ctccgacaac ctcacgcacc acggcctgag aaatatcaaa aagatttttc
      961 ctcacgtccc tcactttaaa cagatatttc tgggcatcat cgatttgata ctgaatgatc
     1021 cattctacat ccactacgtt cagatccccc gtgagcatgg ttttttcggg gccataatcg
     1081 cctgcccgat atgtggattg aactccagcc ttctctgtgc gaaatccaaa ttccatcttt
     1141 ttgattttgc gcacctcaac cttgctcacc gtttccaaac caaagggaag attgaagtgc
     1201 agacccggct tcacggtacg attgaatttg ccaaatcgca aaacaacggc atcttcatcg
     1261 ggcgccacag tataaaccat gttgggcaaa gccaacacga gcagaaatac aatcacaggg
     1321 atcagagaaa gtttctttaa
//
LOCUS       DATDUN010000186         1155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_548088, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000186 DATDUN010000000
VERSION     DATDUN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548088"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VJC18_02470"
     CDS             complement(<1..1132)
                     /locus_tag="VJC18_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44275.1"
                     /translation="MYEESALDPGFGRIHHDETMMVLRTAYYENVFPRIDQIAGLTKH
                     FPGGDGQYLFGGMFFDWLAKRYGEERMYKFQKEYASSLWLFSLNNKARRVYGKSFYKL
                     YKEFKSDYTAQFDAQQHVLSAQGVTPLEDVVKNGDSQTQYTPTPYPGGYAYFEESLDK
                     PSAIHLLTKKFDDTASETITFDAEQKLKRSIFGQMSFSKSGRYLAFSALSRVKPQTLL
                     SEVYYYDLKKKTLMRVLDTENPKQSLRVMDPDFSPADGGQRWLVMVRNFLGTDQLYIY
                     DLFEKKGYVITNEPEKTQLSNPRFSPDGSKIAVSRKDPLTGHRDIVVYTNQGSRLVQI
                     TNDIKPDQHPIFSNDGRFVYYDSYKTGIANIFRHELLTNTTTQ"
BASE COUNT          261 a          257 c          319 g          318 t
ORIGIN      
        1 cctgtgttgt ggtgttggtc agcagttcat gacggaaaat attggcaatg ccggttttgt
       61 aagaatcgta ataaacaaaa cgcccatcgt ttgagaaaat ggggtgctga tcgggtttga
      121 tgtcgttggt gatttgcaca agccgactac cctgattggt gtagacaacg atgtcgcgat
      181 gacccgtgag gggatcttta cgactcactg cgattttgga cccgtccgga gaaaagcgcg
      241 ggttggacag ctgtgttttt tcgggttcgt tggtgatcac gtatcccttt ttttcgaaga
      301 ggtcatagat gtagagctga tctgttccca aaaagtttct caccatcacg agccagcgtt
      361 gcccgccgtc tgccggggaa aaatcggggt ccatcacgcg cagggattgt ttggggtttt
      421 cggtgtcgag cacacgcatg agcgttttct ttttcagatc gtaataatag acttcggaga
      481 gcagtgtttg tggcttgacc cgtgacaagg cggaaaaggc cagatagcgg cccgatttcg
      541 aaaaagacat ttgaccaaaa atggaccgtt tgagtttttg ttctgcgtca aaggtgatcg
      601 tttcggatgc cgtgtcatcg aatttttttg tgagcaggtg gatggcggag ggcttgtcga
      661 ggctctcttc gaaataagca taacctccgg gataaggtgt tggcgtgtat tgtgtttgcg
      721 aatctccgtt cttgacgaca tcctctagcg gcgttacgcc ttgggccgac aacacatgtt
      781 gttgtgcatc aaattgggcc gtgtagtcac ttttgaattc cttgtagagc ttgtaaaaac
      841 ttttgccgta aacccggcgg gccttgttat tgagggaaaa aagccacagg ctggaggcat
      901 actctttttg aaatttgtac atgcgctctt cgccgtagcg cttggccagc cagtcaaaaa
      961 acatgccgcc aaacaaatac tgcccatcgc ctcccggaaa gtgtttggtg agaccggcga
     1021 tctgatcgat gcgcggaaag acattttcgt aataggccgt tctcaagacc atcatggttt
     1081 cgtcgtggtg tatgcggcca aacccagggt cgagtgccga ttcttcgtac acagccatgc
     1141 cttcacgcat ccaac
//
LOCUS       DATDUN010000187         3971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_506893, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000187 DATDUN010000000
VERSION     DATDUN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506893"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VJC18_02475"
     CDS             complement(<1..596)
                     /locus_tag="VJC18_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44276.1"
                     /translation="MQQKFRYQWFAMGDLNAFFGLMLDNVTNLVLLWGLLAVAGFPSH
                     VYFYLMVPGTALGVMFGDIVYTVMAFRLAKKTKNPHVTAMPLGLDTPSTIGVALAVLI
                     PCFVAAKGRGLSVEDAAFVTWQVGMATMIFMGIFKVIMSFAGEWVQRVVPRAGLLGSL
                     AGIGVTLLGYIPLQHVYGLPEVGLVAFGLVIYTLVAHH"
     gene            708..1643
                     /locus_tag="VJC18_02480"
     CDS             708..1643
                     /locus_tag="VJC18_02480"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HLD44277.1"
                     /translation="MLASTFAATTSPMYAFMCPGQARVEPQTELDIATGWPGYGHLLE
                     EAENTLAFDPIDEVIYRNSANQTRTSLIQPAVLTLTTARGLCLQEKGIRPSAVLGHSL
                     GQYSALVLAGVLDFADALHIVYRRGLMMEEIIRLTPGSMLAITSADRDTIEGILPEAG
                     EQGCVVVAGVNSSRQTVVSGASTAVDRIRQIVEERHLGTPVPLCVQVPFHSPILHRMR
                     EDFARLLNGFIFHPPQMAFIDNVTGQRVDDPEIIRANLVEHLTSPVLWADCVRAAHAL
                     GCSVFLELGHKRMLAQFNKHIVTGQQTLLWNELLV"
     gene            1975..3132
                     /locus_tag="VJC18_02485"
     CDS             1975..3132
                     /locus_tag="VJC18_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44278.1"
                     /translation="MITTTPMTVQVTPTTLPSALTQSTGTTFVPPTTQAVLLAHKGAI
                     GRMVQTDPTALVSRLARDPIVPRSQYLRHHRTFHSPMSTFTDKLDQTQLSRVYLENQH
                     ENTLACAAIGFIAGTFCGAITGVTFDNPLLGGIVAGGSILAGLMPMAKGIIADRKTRR
                     EIQTMIDQALALDFDDIKQTATDGDLVTVCHYIIDILDLRVRDERTGYGFWNDIIHAT
                     YSLTTIMKRLADDQQTGSSDNDTHLLPLLMPKLKQLHQILSDINESAKALHYLHPQGT
                     ITSMRDVDLDNIGVAVCGALRIPDESDHIPHEKIIHALDIIQRSDQGLSAILIAFQNA
                     QQLIVEIKSILNEHGRTISDLPDLGYAIPLLYGIRELLKIMHDKLGEQVRL"
     gene            3337..3915
                     /locus_tag="VJC18_02490"
     CDS             3337..3915
                     /locus_tag="VJC18_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44279.1"
                     /translation="MGNEITIYTQADKHDWLVTGYRLEDPLTQQPYVTYEGTAYSPDA
                     KFRCAIPYGTRDLGQALSVCDAAADAFVPAPAPEPAEGQEAQAEQTDPYTQTYEVLLH
                     GYNNYAGKVRRWLDYHDRTVEGTSFIYYDPKCAPELKNREQGRCAYVVDSDTAKKGME
                     EFYFPPATSGYSALLFQGGKRASFVDLINDQL"
BASE COUNT         1101 a          997 c          916 g          957 t
ORIGIN      
        1 tcatgatggg ccacaagagt ataaatcacc aacccgaaag ccaccagccc cacctcgggc
       61 agaccgtata catgttgcag ggggatgtag cccaaaagcg tgacaccaat cccagcgagt
      121 gaacccaaca gtccggcacg cggcaccacg cgttgcaccc attctccagc aaacgacatg
      181 atgactttga aaatccccat gaagatcatt gtggccatcc ccacctgcca ggtcacaaac
      241 gccgcatcct caacactgag ccccctgccc ttggccgcta cgaagcacgg gatcaggacg
      301 gccagggcca caccgatagt ggatggcgtg tcgagcccca aaggcatggc tgtcacgtgg
      361 ggattttttg tttttttggc cagacgaaac gccatcactg tgtatacgat gtctccaaac
      421 atcacgccca gagcggttcc gggaaccatc aaataaaaat agacatggga aggaaatccg
      481 gccacggcca acaggcccca caacaggacc agattggtca cgttgtcgag catgagacca
      541 aaaaaggcgt tcaaatcccc catggcaaac cattgatatc taaatttttg ttgcatgacg
      601 gagacaattt gttaaatttg tgcttttgta aagcgtattt tattatcaga ctctttactt
      661 ctaactgcgc atattgatag gagcaattat ctgcgaaagg acaatctatg ttagccagca
      721 cctttgccgc aacaacaagc ccaatgtatg ccttcatgtg tcctggacag gccagggtcg
      781 aaccccaaac cgaactcgac atcgccaccg gttggcccgg ctatggacat cttttggaag
      841 aagcggaaaa caccttggcc tttgatccca tagatgaggt gatctaccgt aactctgcaa
      901 atcagacacg caccagtctc atacaaccgg ctgtgctcac gttaacaacg gcaagaggcc
      961 tctgtttgca agaaaaaggg attcggccca gcgctgtttt gggacacagt ctcggtcaat
     1021 attccgcttt ggttttggca ggcgtgctcg actttgcgga cgctcttcat attgtctatc
     1081 ggcgtggact catgatggaa gaaatcatcc gcctcacccc cggaagcatg ttggccatca
     1141 cgagcgccga tagagacacc atcgagggca ttttgcctga agctggagaa cagggttgtg
     1201 tggttgttgc tggcgtcaat tcaagcagac aaactgtggt ctctggagca agcactgcgg
     1261 tggatcgcat tcgccaaatt gtggaagaaa ggcatttggg cacacctgtt cccctttgtg
     1321 tacaagttcc ctttcactca cccatcttgc acaggatgcg agaagacttt gcccgcttat
     1381 taaatggatt catcttccat cccccacaga tggcgtttat cgacaatgtc acaggtcagc
     1441 gagtagacga tcccgaaatc attcgcgcca atttggtgga acacttgacc agccctgtgt
     1501 tgtgggcaga ttgcgtcaga gccgcgcacg cgctgggatg ttcggtgttt cttgagttgg
     1561 ggcacaaacg catgctggcg cagttcaata agcacatcgt taccggccag caaactttat
     1621 tgtggaacga gttgctcgta tgacggagac acgcttctca catgtcgaga tgatacgcca
     1681 aaatactccg tcatgataac caaccacttt cacccccaaa acaataatta gacggaaaca
     1741 cttgagatat tccgtcaaca actctgtaga cactttctgc acgttctaca caacaatcag
     1801 ccttttcaac cgataatgtc agagaacgca caaataaaag gagccagctc atagcattga
     1861 ttacaacttg gtggcgcgtc aactatcagc aggcggtgaa gtcatggtcg tgtgtgagga
     1921 tggagaattg caaaaccagg tcacacgtaa ggaccaaaac aaagggatta ttctatgatc
     1981 accacaaccc ccatgaccgt tcaagtaaca ccaaccactc tgccttctgc gctaacacaa
     2041 agcactggca caacctttgt tccgccaaca acccaagctg tgcttctcgc tcacaaaggg
     2101 gcgatcggtc gcatggtgca aaccgatccc acagcgttgg tgagtcgttt ggccagagat
     2161 ccgatcgttc ctcgctcaca atatctacgg caccaccgaa cctttcacag tccaatgtcc
     2221 acttttacag acaaactcga ccagactcag ttaagtcggg tatatcttga gaatcaacat
     2281 gaaaacacac ttgcctgcgc cgcaatcggt tttattgcgg gaactttctg tggcgcaata
     2341 actggtgtga catttgacaa ccctctgttg ggcggtatcg ttgccggcgg ctctatactg
     2401 gcaggtctaa tgcccatggc aaagggcatc attgctgatc gaaaaacccg tcgagagatt
     2461 cagaccatga tcgatcaggc tctagccttg gattttgatg atatcaaaca aacagcgacc
     2521 gatggcgatc tcgtgaccgt ctgtcattac atcattgata ttttggatct cagggtaaga
     2581 gacgaaagaa caggttatgg attctggaac gatatcatac acgcaacgta tagcttgaca
     2641 actatcatga aacgacttgc agatgatcag cagacaggat cctcagataa tgatacacat
     2701 ttgttgccgt tgcttatgcc aaaactgaaa cagttacacc aaattttgtc cgacatcaat
     2761 gaatctgcca aagcccttca ctatctgcac ccacagggaa ccatcacatc gatgagagat
     2821 gtggatttag acaacattgg cgttgctgtg tgtggggctc tgcgcatacc cgacgaatcc
     2881 gatcatattc ctcacgaaaa aataattcac gctctggaca tcattcaacg ctccgatcaa
     2941 ggactctcag caatcctcat cgcttttcaa aatgcccaac aactgattgt tgagatcaaa
     3001 tctattctta acgagcatgg tcgtacaatc agtgacctgc ccgatcttgg ctatgctata
     3061 ccacttttgt acggcatcag ggaactcttg aagataatgc acgataagct gggtgagcag
     3121 gtacggttat gaatattatt ttcctatgac ggagaccata ttattttcct atgagcttga
     3181 tctggccaca aagacattta gagaaattta agaaaataaa ggcaaaagta tgtgacacgt
     3241 tgcgacattg aagacttgtt ttttagcaac ttcttcattt atatgccgat agttgcatgt
     3301 agcagtgatg tataacaatt ttaaaaggat gttattatgg gaaatgaaat cacaatttat
     3361 actcaggccg acaaacatga ttggctcgta acaggttacc gtctggaaga tccccttaca
     3421 caacaacctt acgtgaccta tgagggaaca gcgtatagtc cagacgcaaa gtttagatgt
     3481 gccatacctt atgggacaag agatcttggt caggcgctct ctgtctgtga tgcggccgca
     3541 gatgcttttg ttccagcgcc tgcacctgaa ccagccgaag gtcaggaggc tcaagcagag
     3601 caaacggatc cctatactca aacatatgaa gtcctactcc atggttataa taattatgct
     3661 ggtaaagtca ggagatggct tgattaccat gatcggacag ttgaggggac ctcgtttatc
     3721 tattacgatc ccaaatgcgc gccagaattg aagaatcgtg agcagggacg atgtgcctac
     3781 gttgtggact cagatacggc aaaaaaaggg atggaagaat tctattttcc accggccacc
     3841 tcaggatact cagctcttct gtttcagggg ggcaagagag cttccttcgt cgatctgatc
     3901 aatgaccagt tgtagtcaag aagatgctgg ggggttcggg tcttgactag ggacttgact
     3961 ccaataacga t
//
LOCUS       DATDUN010000188         1065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_548267, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000188 DATDUN010000000
VERSION     DATDUN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548267"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VJC18_02495"
     CDS             <1..253
                     /locus_tag="VJC18_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44280.1"
                     /translation="LYIKVKRIHLLPQVTTKQGAREQPIELRIFGQIKEKKTDQILVK
                     FYDATTSSFTLGEDEAERALKNLSQSLMGDLAQYLKTYY"
     gene            255..>1065
                     /locus_tag="VJC18_02500"
     CDS             255..>1065
                     /locus_tag="VJC18_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLD44281.1"
                     /translation="MKTIIFLDAGSFDYTGVDLSGIRALGRCLTYHSTKPEQVESRIQ
                     KADVVIVNKVVLDKTHLENAKKLQLICVAATGYNNIDLNAAKKCDIAVCNTPYYSTRS
                     VVEHTLMFLFALSHRLIEHHEACQKLKWSRSSTFNLTAFPFSNLNGKTLGIVGYGAIG
                     KSVARLAKHCGMNILVAKIPGRKHDRKTKRTPLKTVLQQSDYVSLHCPLSPLTTRLVN
                     RETLSLMKPTAFLLNLARGPVVDETAVALALLDNQIAGYAADVLSQEPPKKD"
BASE COUNT          301 a          291 c          235 g          238 t
ORIGIN      
        1 actgtacatc aaggtcaaaa ggattcacct gcttccgcaa gtcactacaa agcaaggcgc
       61 ccgtgaacaa cccattgagt tgagaatttt tggacaaatc aaggagaaga aaacagatca
      121 gattctggtc aagttttacg acgccaccac cagctccttt accctgggag aagacgaggc
      181 tgagagagcg ctcaaaaact tgtcacaatc actgatgggc gatttggcac aatatctcaa
      241 aacatattat tgacatgaaa accattatct ttctcgatgc cggatccttt gactacacgg
      301 gcgtggacct ttctggcatc agggccttgg gccgctgtct cacctatcat tccacaaaac
      361 cggaacaggt tgagagcagg attcaaaagg ccgatgtggt gattgtcaac aaggtggttc
      421 tggataagac acatctggag aatgcaaaaa aacttcaact gatctgcgtg gccgcaaccg
      481 gttataacaa catcgatctc aatgcggcca aaaaatgcga cattgctgtc tgcaacacac
      541 cctattactc tacacgctcg gtcgttgaac acaccctgat gtttttgttc gcactctcgc
      601 acagactcat tgagcatcac gaggcctgtc aaaaactcaa atggagtcgc tcctcaacct
      661 tcaacctgac tgcctttcct ttttccaacc tcaacggcaa aaccttgggg atcgtgggtt
      721 atggcgccat tggaaaatca gtggctcgtc tggcaaaaca ttgcggcatg aacattttgg
      781 tcgcaaaaat cccggggaga aaacacgatc gaaaaacaaa aagaacgccg ctgaaaaccg
      841 tgttgcaaca aagcgactat gtctcgcttc actgcccctt gtctccccta accaccaggc
      901 tcgtcaatcg cgaaaccctg tcactcatga aacccactgc gtttttactc aatctggcca
      961 gagggcccgt ggttgacgaa acggccgtcg ccttggcact gctcgacaac cagatcgccg
     1021 gttacgccgc ggatgttctg tcgcaggagc ctcccaaaaa agacc
//
LOCUS       DATDUN010000189         4754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_259030, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000189 DATDUN010000000
VERSION     DATDUN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259030"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1459)
                     /locus_tag="VJC18_02505"
     CDS             complement(200..1459)
                     /locus_tag="VJC18_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HLD44282.1"
                     /translation="MKFLKSSSVFFAIFCAVFMLCTSRTPLFAQNPDAPVNTAMQQLV
                     SANTEFAVNLYAKYASLKENIFFSPYSISSALGMTYEGAQGETAKEMEKVFGFIQEAQ
                     KRREGTLSLFSEINKKDKQYELSTANALWVQAGYKLLDSYTDIVNKFYGSNITPLDFV
                     NKAEESRLTINSWVEKQTHEKIKNIIPPPLPSGVSLILTNAIYFLGNWEKQFDITATS
                     DQKFWITPATSVKAPMMTFAQNPVYRGYTETETYQALNLPYKSEDLSMMIILPKSRDI
                     KKLEAILSDSVISDCQQKTKNRMNVVVSIPKFKIETTYSLTEPLAGLGMKTAFQYPAA
                     DFSGIDGTHNLFISKIIHKAFVEVNETGTEAAAATAIMVEAGASPGPRPVIPVFVADH
                     PFLFVIQQQATGNILFMGRVLDPTKSL"
     gene            complement(1565..2068)
                     /gene="traF"
                     /locus_tag="VJC18_02510"
     CDS             complement(1565..2068)
                     /gene="traF"
                     /locus_tag="VJC18_02510"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HLD44283.1"
                     /translation="MKTKCKLNRSFLLALSLSLSLLLSCSQRPSPISYQEVDSKLQSA
                     KTTGALIFIYSTDCKLCLEQIPVVNKIKDSLKQNGFEVLAVSIDDIEDKFSTYLNKNK
                     VSFKPLILKNKAVGGLTSLLARYSGLYKNSVPYSAVMNRSGKIIKEWTGHASFEEYVH
                     WIQKAAN"
     gene            complement(2116..2787)
                     /locus_tag="VJC18_02515"
     CDS             complement(2116..2787)
                     /locus_tag="VJC18_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44284.1"
                     /translation="MTMKYIKPSLLITGVLLLCLFWAACARANTTTVNFELKRVTNNG
                     QPPIFADGTYSLGDYQATVSQGVYAGLAIGFSIQQADKSWLRFNLDIPNTQHLSVWEK
                     NYDQKTGFQFEYKIGPLPLKLKPADLVVEADARLISPKHYWKPRLYIEKTTEGIASKP
                     YIQFDDFDLTITAFSIQGPRLIMKAEFTGKINDPAMLKTVGAYEIKGTLTIDSSKEIT
                     IMMTD"
     gene            complement(3078..>4754)
                     /locus_tag="VJC18_02520"
     CDS             complement(3078..>4754)
                     /locus_tag="VJC18_02520"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44285.1"
                     /translation="YAQTEVHTEQGGASFEEVRGRIFKTIDGGENWSLVWDGNALTRY
                     LIIDPENTDTLYVSTGIFDREAYDSDCANDIQGGVGVLKSTDGGQTWAQSNNGLNDLF
                     VGSLRMHPTNHNILFAATGSISCSGAGHMGLYKTTNGGTSWTQVIDDAILTTVNFSLS
                     NPSTMYAGGYGGFFKSTDGGTTWTEYTRGSLKTWGPEGVNGGFPIDVTVHPDDPDLVY
                     VNAYGGGVLRSTDGTQTWELWSNGFSGAILYDVEVPSYDSSTVYAIGRSGPFKSTDYG
                     ETWTGIANYDHIANEWMTVATHPDNPDLVFISIEGESIVYRSDDGGTSFEQIIADDEP
                     ESGIKSFAFAPSDPEVIYGCVAKADLYPSESYQNIAHGKGLYKSTDGGQYFSLIDTNL
                     TDVNWRRIVVDPDDADTAYAATSSGLYKTTDGGTHWTRFAGLGDKFLADVIIDFDNDY
                     FVVSEMFGGVYVSDDAGVTWTGPNNTGMNSGNPYVAALVFDPEDIDIIYAAETYSGVY
                     QSTDQGLTWSPFPDDDMSGLDYHAVKDIVITDAALYVATQGAGVFRYVRE"
BASE COUNT         1153 a         1119 c         1169 g         1313 t
ORIGIN      
        1 ctcagcgaca taagtccgat acatcttttg atgctgtgtc gttcattgcg gtgcctgctc
       61 acgtacttgc gtgtacgctc cgctggctcc tcatttcact ccttgcctca aaagcgtctc
      121 gaacttatgt cgctgagtat agaggtcccc ttaagaatag aatgtgaaga tgcctggcag
      181 cctgtgctga atcggtgggc taaagacttt ttgtaggatc aagcactctg cccataaata
      241 gaatattccc cgtcgcctgt tgttgaatca caaagaggaa cgggtggtca gcaacaaaaa
      301 caggaatgac aggtcttggt cctggtgaag cccccgcctc aaccatgatt gccgtggccg
      361 cggcggcctc tgtgcctgtt tcgtttactt ccacaaaggc tttgtggatg atcttgctga
      421 tgaagaggtt gtgcgtgcca tcaattcccg aaaaatcagc ggcggggtat tgaaaggcgg
      481 ttttcattcc caatcccgcc agcggttcgg taagagagta ggtggtttct attttgaact
      541 tgggaattga tacaaccacg ttcattctgt tctttgtttt ttgttgacaa tctgagatga
      601 cagaatccga gagaatcgct tcgagctttt tgatgtccct tgattttggc aggataatca
      661 tcatggataa atcttcgctt ttgtacggaa ggtttaaggc ttggtacgtc tctgtttcgg
      721 tatagcctcg ataaacagga ttttgcgcaa acgtcatcat cggggctttg accgacgtgg
      781 ctggtgtgat ccagaacttt tgatcggatg tggctgttat atcgaattgt ttctcccagt
      841 ttcccaaaaa ataaatcgcg tttgtcagga taagactgac gccggagggc aggggtggcg
      901 ggatgatgtt cttgatcttc tcgtgcgttt gtttttccac ccaggagttg atggtaagac
      961 gtgattcttc ggccttgttt acaaagtcaa gtggggtgat gttgcttcca taaaacttat
     1021 tcactatgtc tgtatagcta tccaaaagtt tgtaaccagc ttgaacccac agggcgttgg
     1081 ctgtggagag ttcgtattgt ttgtcttttt tgttgatctc actaaaaaga gagagggtgc
     1141 cttcacggcg cttttgtgcc tcctgaatga agccaaacac cttttccatt tctttggctg
     1201 tttcaccttg ggcgccttcg tatgtcatgc ccaaggccga tgagatgctg tagggtgaga
     1261 aaaaaatgtt ttccttgagt gaggcgtatt tggcgtaaag gtttacggca aactctgtgt
     1321 tggccgatac gagctgttgc attgccgtgt tgacaggcgc atccggattt tgagcaaaca
     1381 gtggagttct tgatgtacaa agcatgaaca cagcacaaaa aatcgcaaaa aacacactag
     1441 aagatttaag aaatttcata attggttcct gcttaaagac cctttctctt tccaaacgta
     1501 tcacgaataa tccaataata taacaatgtc ggcatcatgg ttgggcaccc tttattgcta
     1561 atgctcaatt ggctgctttt tggatccagt gcacatattc ctcaaaactg gcatgcccag
     1621 tccattcttt gatgatcttg ccggaacgat tcatgacggc cgagtaaggg acagaatttt
     1681 tatataagcc actatagcgg gccaacagag aggttaggcc gccaacagct ttgtttttta
     1741 agattagcgg cttaaaagaa accttgttct tattcaaata agtactaaat ttatcctcaa
     1801 tgtcatcgat agacacagca agtacttcga acccattttg ctttaaactg tctttgatct
     1861 tgttcacaac aggaatttgt tcgaggcata gcttgcaatc tgtgctgtat ataaaaatga
     1921 gggcaccagt ggtctttgcg ctttgaagct tgctgtcgac ctcttggtaa gaaatggggg
     1981 aaggtctttg tgaacagctc agcaataatg acagactaag tgacagagct aacagaaaag
     2041 acctgttgag cttgcatttt gttttcataa gttgattcct ttgtttatgc tggggtcaat
     2101 agtttgcatg ccccgttaat cagtcatcat gatggtgatt tcttttgatg agtcgatggt
     2161 gagagtgcct ttgatttcgt acgcccccac ggttttgagc atggcagggt cgttgatttt
     2221 tcctgtaaat tccgccttca tgatcagccg tggcccctgg atggaaaagg ccgtgatcgt
     2281 gaggtcaaaa tcgtcaaact gaatgtaggg tttgctggcg atcccttcgg ttgtcttttc
     2341 gatatacaat cggggcttcc aataatgctt gggtgagatc aaacgggcat cggcttcgac
     2401 gaccaggtcg gcaggtttga gtttgagggg gaggggacca attttgtatt caaattgaaa
     2461 gcctgttttt tgatcgtagt ttttttccca cacagacaaa tgttgagtgt tggggatgtc
     2521 caaattgaat cgcaaccacg atttgtcggc ttgttgaata ctgaaaccaa tggccaagcc
     2581 ggcatacacg ccctgactca ctgtggcctg gtaatcacca agagagtacg tgccatccgc
     2641 aaagattggc ggttggccgt tgttggtgac gcgcttgagt tcaaaattga cggtggtggt
     2701 atttgctcga gcacaagccg cccagaaaag gcagagaaga agaactcccg tgatgagcag
     2761 tgagggtttg atatatttca ttgtcatgct accttctgac acagttgatt aagataatca
     2821 aacaaaccat tcattctggg tgtacgacat aaatgtgggt gatttgaatt gagtaccccc
     2881 actcctgttg tcaggagggg gtggctgaaa gccgggggag gtagaaaatt tgggaatttc
     2941 ccatctctca gctaatcacg atactacctc cccaccctgc gctgctgcgc agggagcccc
     3001 gccttacaaa agcggggcgg cgccaaagtt taccatcaat gatttacaat ttacccacaa
     3061 atatgtcgta cgcccattca ttctcgtaca tatctaaaaa caccggctcc ctgcgtggcc
     3121 acataaaggg ctgcatccgt aatgacaata tccttgacag cgtgataatc cagaccagac
     3181 atgtcatcgt caggaaaagg agaccacgtc aacccctggt cagtcgattg atacaccccg
     3241 gaataagttt cggcggcgta gatgatatct atgtcttccg gatcaaagac cagggctgcc
     3301 acatagggat tcccgctgtt cattcccgtg ttgtttggtc ctgtccaggt gacaccggca
     3361 tcatcgctca catacacgcc accaaacatt tcgctcacta caaagtagtc gttatcaaaa
     3421 tcaatgatga catcggccag aaatttatcg cccaatccgg caaaacgggt ccagtgtgtg
     3481 ccgccatctg tggtcttgta gagccccgat gatgtggcgg cgtaagcggt atcggcatca
     3541 tccggatcca caacaatgcg gcgccaattc acatcggtca gattggtgtc gatcagtgaa
     3601 aaatattgtc ctccatcggt ggatttatac aagcctttgc catgagcaat gttctgatag
     3661 ctctctgacg gatacaaatc cgccttggcc acacagccat aaatgacttc gggatctgag
     3721 ggggcaaagg caaaagattt gatcccgctt tctggctcat cgtccgcaat gatttgttca
     3781 aatgaagtcc ctccatcatc actgcgataa acaatgcttt ccccttcaat gctgatgaac
     3841 accaagtcgg ggttgtccgg gtgtgtggcg accgtcatcc attcattggc gatgtgatcg
     3901 taattggcaa tccccgtcca ggtttcgccg taatcggtgc tcttaaaagg cccactgcgt
     3961 ccaatggcat agaccgtgga cgagtcatat gaggggactt cgacatcata cagaatggcc
     4021 cccgaaaatc cgttggacca taattcccag gtctgtgtgc cgtctgtgga tcgcaacacg
     4081 ccgcccccgt aggcattgac gtaaaccaga tcgggatcat ccgggtgtac ggtcacatca
     4141 atgggaaacc ccccattgac gccctctggc ccccaggtct taagactgcc ccgggtgtat
     4201 tcggtccagg tggtgccgcc atctgtgctt ttaaaaaaac cgccatagcc cccggcatac
     4261 atggtggaag ggtttgagag gctgaagttg actgtggtca aaatggcatc atcaatgacc
     4321 tgtgtccagg aggtccctcc gtttgtggtt ttatacagcc ccatgtgtcc cgcgccagaa
     4381 caactgatgc ttcctgtggc cgcaaacaga atgttgtgat tggtggggtg catgcgcaga
     4441 ctgcccacga agaggtcatt caggccatta ttgctctgtg cccaggtttg tccgccgtcc
     4501 gtgcttttga gcacccccac accgccttga atatcgttgg cacaatcaga atcgtaggcc
     4561 tcgcgatcaa aaattcccgt ggagacgtaa agggtatccg tattttctgg atcaatgatg
     4621 agataacgtg tcagcgcgtt gccgtcccac acaagcgacc aattttctcc cccgtcgatt
     4681 gttttaaaaa tgcgacccct gacttcttcg aacgaggcgc ccccctgttc cgtatgcacc
     4741 tctgtttgcg cata
//
LOCUS       DATDUN010000190         3516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_465488, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000190 DATDUN010000000
VERSION     DATDUN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465488"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1824
                     /locus_tag="VJC18_02525"
     CDS             229..1824
                     /locus_tag="VJC18_02525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HLD44286.1"
                     /translation="MTQTTSVTVTTEQWQQIYDYLRPIAGTNKNDCSLPADSCVTQND
                     QIDCCLSANDLVGVNLDPNTAPFKINGVWYGDVANLPTLYAKAVALDRNDGSFDGVLQ
                     TTETTTSVTLKLSFAQYDKSIFKITREDTSYGGVGNGWIVEEEPLEGGQYRHWLVTTA
                     SVVDDDDAMETSWQIDVGKEKINLTAAGVDHCSNVGVLYFDSGDRLGTVPLGEEVVSK
                     TDSVIVYGFQDGETPSESSGTVNNLEKYSYQATPAVVQFGATTVNENEGSPVFNTSGY
                     CVGMSLRPNEDQVSLFLPINEVRRVYQQIRAEGVATHGYSGLLSYSRFLEKEELISLG
                     VTGRSYGLIIQDTPLANNFFSQLEVGDIVLQYDGKSVPDANNIQRMDAYFATITDRPV
                     ILHVLRGGEELDVEAQSVVMHQPLYPTYKTEFTTEEGQKFILKEVDRDYLNLRWDVLY
                     EENHFLIVEMDSGDGVYLSKGILTSVNTGPVNSIEEFRAIVDGKEDVKKFVFAGLTPN
                     VIQPDYFSTFIISCANPRYREKF"
     gene            1840..3198
                     /locus_tag="VJC18_02530"
     CDS             1840..3198
                     /locus_tag="VJC18_02530"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HLD44287.1"
                     /translation="MTTMTSTNLLVQPGSGTQSPDSLAFEPDNKAWGTMIGKYQDSVL
                     AIKTVSRFGAGQGTAWVVEKEDLGNGLFRHWCITNAHVVDDELNQEMYYSVFVNNQEF
                     YEPVPAHVQGVDHVNDVAVVWFDSRIILEPMPVDETAKVFKGDEILVIGNQQGEGVIP
                     SLGNVNNVDVYGWSAVEAFQDDTSALPGNSGSPAINAQGRVVGLHNSGRRGVNGMGYN
                     ILISPVMESYRSMRKNGFVVHGRVGEYSGDWVTQDKLELLGVQGYANGLVLGYLPQWH
                     AFYRAGVRSGDVLLGYGNRPLPTNEDIDPVTSFFVHQPKQCASVTIAREGKIKTVKVR
                     IGSYEEKQRKILETQYGYAFAEVDRFFMQDEFNVDVEPGGLALIDQGDGLATGVLTSL
                     NNTPVGTVTEAQEAIDSATGPYILLSFVFYIQGRVQQSLIQLNNANYVAPSNSSPENN
                     SK"
     gene            complement(3153..>3516)
                     /locus_tag="VJC18_02535"
     CDS             complement(3153..>3516)
                     /locus_tag="VJC18_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44288.1"
                     /translation="AVTRFVPVCKKNNLTFLRVFPLTGRTNQIRVHVNVAGGHILGDP
                     LYGGQVSTSDQLRSDQNNTTQTCTMSRLDPMFDPMFLHCRAMTFKPAIGADPVTFTAD
                     WPEHYLELFSGDELEGAT"
BASE COUNT          919 a          702 c          965 g          930 t
ORIGIN      
        1 ctttagatga tgatgaaatt tgtctcgggc catcctcatg gctatattat atattgcaca
       61 atgtaaatgt gcaatatata acttgtctgt atgacggagg aatgattggc ctgcctaacc
      121 gactgaaatt ccatgttttt atttttagca acttttcccg gaagacgtgc gatatttgac
      181 caagcgtgca gagcacaata aaagcgtgta tgacaacaag gggcatctat gacacaaacc
      241 acttcggtca ccgtgacgac ggaacaatgg caacagatct atgattattt aagacccatt
      301 gcggggacca ataaaaacga ttgctcgcta ccggctgaca gttgtgtcac tcaaaacgat
      361 cagattgatt gttgtctttc ggcaaacgat cttgttggtg tgaacctcga tcccaacacc
      421 gcaccgttca agatcaacgg cgtttggtac ggggatgttg ccaatctccc cactctttac
      481 gcgaaagcgg ttgcacttga tcgaaatgat ggttcctttg atggtgtttt acagacgacg
      541 gagacgacca catcagtcac tttaaaactt tcttttgccc agtacgataa aagcattttc
      601 aaaatcacca gggaagatac ctcttatggc ggtgtgggga atgggtggat tgttgaagaa
      661 gagccattag aaggtggtca atacaggcat tggctggtga cgacagcgtc cgtggttgac
      721 gatgatgacg ccatggaaac cagttggcag attgatgtgg ggaaagaaaa gatcaatctc
      781 acagcggccg gtgttgatca ttgttccaat gtgggtgtgt tatactttga ttcgggggat
      841 cgcttgggga ccgtgcctct tggcgaggag gttgtttcca aaacagacag cgtgattgtt
      901 tacggatttc aggatggaga aaccccctcg gagtcctctg gcactgtcaa taatctggaa
      961 aaatattctt atcaggcgac tcctgccgtg gttcaatttg gtgccacaac agtgaacgaa
     1021 aacgagggtt cgcctgtttt taatacgagc ggttactgtg tgggaatgag cctgcgacct
     1081 aatgaagatc aggtcagtct tttccttccg atcaatgagg ttcgcagagt ctatcagcaa
     1141 atcagggcgg agggcgtggc cacgcatgga tacagtggtc tattaagtta ttcccgtttt
     1201 ttggagaaag aagagttgat ctcactaggg gtcacagggc gctcatatgg tttgatcatc
     1261 caagacacac cactggctaa caattttttc agccaattgg aagttggcga cattgtgctt
     1321 cagtatgatg ggaaatctgt gcccgatgcc aataacatcc aaaggatgga tgcctatttt
     1381 gcgacgatta ccgatcgtcc ggttattctg catgttttgc ggggaggaga agagttggat
     1441 gtggaggcac aatctgtggt gatgcatcag ccgctttacc ccacgtacaa gacagaattt
     1501 acaacggaag agggtcaaaa atttattctc aaggaggttg atcgggatta tctgaatctg
     1561 aggtgggatg tgttgtacga agaaaatcat tttttgattg tcgaaatgga ctcaggagat
     1621 ggtgtgtact tgtcaaaggg gatattgacc tctgtgaata ccggacctgt caactcgatt
     1681 gaggaatttc gggccattgt tgatggtaaa gaggatgtga aaaaattcgt gtttgcaggg
     1741 cttaccccga atgttattca accggactac tttagcacct ttatcatttc ttgtgccaat
     1801 ccgagataca gggaaaaatt ttaacaaggg aagatgctca tgaccacgat gacatccaca
     1861 aacttacttg ttcaaccagg ttcgggaaca caaagccctg attctctggc tttcgaacca
     1921 gataataaag cttggggcac aatgataggc aaatatcagg acagtgtgct ggccattaaa
     1981 acggtttcgc gttttggcgc gggacaggga acggcctggg tcgttgaaaa agaagatttg
     2041 ggcaatggtt tgttcaggca ttggtgcatc acaaacgccc atgtggtgga cgacgagctg
     2101 aaccaagaga tgtattattc cgtgtttgtg aacaatcagg agttctacga gccagtgcct
     2161 gcccatgtac agggggtgga tcatgtcaat gatgtggcag tcgtttggtt tgattccaga
     2221 ataattttag agccaatgcc agttgacgaa acggctaagg ttttcaaggg ggatgaaatt
     2281 ctcgttatcg gaaatcagca gggtgagggg gtcatccctt cattgggaaa cgtgaataat
     2341 gttgacgttt acggctggtc tgccgttgag gcttttcagg atgatacctc tgcactgccc
     2401 ggaaacagtg ggtcccctgc gatcaatgca caggggaggg tagtggggtt acacaattcg
     2461 ggtcgacggg gtgtgaatgg gatgggttat aatattctga taagccccgt catggagagt
     2521 tatcgaagta tgaggaaaaa tggttttgtc gttcatggtc gcgtgggaga atatagtggt
     2581 gattgggtca cgcaagacaa gttggagctc ttgggggttc agggttacgc caatggattg
     2641 gttttgggct atctgcccca gtggcatgcg ttttaccggg cgggtgtgag atcgggagat
     2701 gtcttgctgg gttatggaaa tcggcccttg cccacgaatg aagacatcga tcccgtaaca
     2761 tcattttttg ttcatcagcc taagcagtgt gccagcgtga ccattgcacg cgaaggaaaa
     2821 atcaaaacgg taaaagttag aataggatct tacgaggaaa aacaacgaaa gatactggag
     2881 acccagtatg gatacgcttt tgcagaggtc gatcgattct ttatgcagga tgagtttaat
     2941 gtagatgttg agccaggcgg gttagctttg atagaccagg gtgatggtct tgccacagga
     3001 gttttgacca gtcttaataa cacgcccgta ggtaccgtga ctgaggcaca agaggccatt
     3061 gatagcgcga cggggccgta cattcttttg tcgtttgttt tttacataca ggggcgagtg
     3121 caacagagtc ttattcaatt gaacaatgcg aattatgttg cgccctccaa ttcatctccc
     3181 gaaaacaatt ccaaataatg ctccggccag tcggcggtaa acgttacggg atcggcgcca
     3241 atggcgggtt tgaacgtcat ggcccggcaa tgcaaaaaca tggggtcaaa catggggtca
     3301 agtctcgaca tcgtacaagt ttgtgttgtg ttgttctggt cagagcgtaa ctggtccgat
     3361 gtcgagactt gacccccata cagggggtca cccagaatgt ggccacccgc cacgttgacg
     3421 tgaacacgga tctggttggt tctgccggtg agagggaata ccctcagaaa agtcaggttg
     3481 ttttttttgc agaccgggac aaaacgggtc acagct
//
LOCUS       DATDUN010000191         1161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_216006, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000191 DATDUN010000000
VERSION     DATDUN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216006"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VJC18_02540"
     CDS             complement(<1..116)
                     /locus_tag="VJC18_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLD44289.1"
                     /translation="MPLYEYRCQQCGFEFETILKFSDPPITVCPQCGEEKVI"
     gene            275..790
                     /locus_tag="VJC18_02545"
     CDS             275..790
                     /locus_tag="VJC18_02545"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HLD44290.1"
                     /translation="MAGSKNIYITGFMATGKSVTGKLLARKLNRFFVDTDKEVETETN
                     LAIEDIFSSQGESGFRLLERELIARISQRSNLVVSLGGGAILTAENRAIFKKGQLIFL
                     STPFQQIWERLQHSQHRPLAKLNFDTLHQLYQDRLPLYNESDIIIDCAQQDTESICQN
                     ILQRIKEHENH"
     gene            777..>1161
                     /locus_tag="VJC18_02550"
     CDS             777..>1161
                     /locus_tag="VJC18_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLD44291.1"
                     /translation="MKTIEVALKVAPYQIHIDRWDLEAAAQIIHKHLKGNRLFVITNT
                     TIARLYRNKLHQSLGQHFSLEWIVVPDGEQHKTLKTCENIYSKLIQKKAHRDSTLLAL
                     GGGVVGDITGFVAATYMRGIDFIQIP"
BASE COUNT          332 a          309 c          236 g          284 t
ORIGIN      
        1 ttgatgacct tttcttcacc acactgggga cagaccgtga tgggggggtc cgaaaattta
       61 aggatggtct caaattcaaa tccgcattgc tggcacctgt attcgtaaag tggcatagga
      121 ttcctttggg gatcctatag ataataaaca aacccctctt gtcaagatga cctatgcaag
      181 acaatgaaaa aatcaagaaa cgaggccatt cttgacgtgc tttgtcactt tttttccttt
      241 tcattttctc catttcattc taacccaagg ccagatggct ggttcaaaaa acatttacat
      301 caccggcttc atggcaacgg gcaaatctgt cactggtaaa ttattggctc gcaaactgaa
      361 ccgtttcttc gtcgacaccg ataaagaagt cgaaaccgaa accaacttgg ccattgaaga
      421 tattttttcc agccagggtg agtctggttt tcgcctgttg gaacgtgagc tcatcgccag
      481 aatctcacaa cgatccaacc tcgtggtgag tttgggtggt ggcgccatcc tcactgcaga
      541 aaatcgcgcc atttttaaaa aaggtcagct gatttttctg tcaacacctt ttcaacaaat
      601 ttgggaacga ctgcaacatt cgcaacaccg tcctctggcc aagctcaatt ttgacacatt
      661 gcaccagctc taccaggaca gacttccttt atacaatgag tccgacatca tcatcgactg
      721 cgcccagcag gataccgaat ccatttgtca aaacatcctg cagaggatca aagaacatga
      781 aaaccattga ggtcgcactc aaagttgctc cgtaccaaat tcacatcgat cgatgggacc
      841 tcgaggcggc cgctcaaatt attcataagc acctcaaagg gaaccggtta tttgtcatca
      901 ccaacacgac catcgccagg ctctacagaa acaaacttca ccaatcgctg ggtcagcact
      961 tctcgctgga atggatcgtc gttcccgatg gggaacaaca caaaaccctc aaaacctgcg
     1021 aaaacatcta cagcaaactc atacaaaaaa aagcccatcg cgattccacg ctccttgccc
     1081 tcgggggcgg cgtggtcggc gacatcaccg gatttgtagc ggccacctac atgcgcggca
     1141 tcgattttat acaaatacca a
//
LOCUS       DATDUN010000192         1670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_216014, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000192 DATDUN010000000
VERSION     DATDUN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216014"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VJC18_02555"
     CDS             <1..930
                     /locus_tag="VJC18_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HLD44292.1"
                     /translation="LMTLASLALWRLRRSQQRACATITVLLVALLIPVLANAETNRFS
                     IISDVPTLGSSGLVYTLGTQTMDQSWAFSTSLDYQNKPLELSNAGVAQSVSDDLVTAY
                     YAFAWAPADKWQLDVIAPIVVYNKFSTPDVAVVNFNNAWGLGDIFVRLHYRVWQNTDA
                     TKGFSLIPYVTLPTGDEDNYISDATFRGGLKLVGDWQINDKWYTAINVGAELREPVNL
                     LNYAEKGRLLASTGLAYAFTEQVRGMADLVASTAINKPLTEKVTTPVEAMLGLNAKLK
                     KLPIAFNLSGGLALIRGAGTPLFRTLAGMTIGM"
     gene            complement(979..>1670)
                     /locus_tag="VJC18_02560"
     CDS             complement(979..>1670)
                     /locus_tag="VJC18_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HLD44293.1"
                     /translation="NRLLAQYAADVERYLKENYRSLFLSGVGRIADLACQPVKNSQIV
                     STDSPFYRKVPLFLEIMQKWHLVFKAAAQTKHPESSGGIASKRYLFDVGMTSFLIHQN
                     QPVSWRDRSELQNIIYAKLQETFVCQEILALEPEPQMDLNYYRETKNSQEIDFLVPLN
                     GKIIPIEVKSQTSFSKNSLMPMLDFLDHRGLNCGIWVYNGEMRTQRFRGKTIHFIPPF
                     FISELPRVITP"
BASE COUNT          405 a          431 c          419 g          415 t
ORIGIN      
        1 ctgatgacct tggcttcgct ggccctgtgg cgcctgcgtc gctcacagca gagggcctgt
       61 gcgaccatca ctgttttgct ggtggccctg ctcatccccg tgctggcaaa tgccgagacc
      121 aaccggtttt caatcatctc cgatgtgccc acgctggggt catcggggtt ggtgtacacg
      181 ttggggacac aaacgatgga ccagtcttgg gccttttcca cgtcactgga ttatcaaaat
      241 aagcccctgg aactttccaa tgcgggtgtg gcgcaaagtg tgagcgatga tctcgtcacc
      301 gcgtactacg cctttgcgtg ggcaccagcc gacaaatggc agttggatgt gatcgcaccc
      361 atcgtggtct acaacaagtt cagcactccc gatgtggctg tggtgaattt caacaacgca
      421 tggggactgg gcgatatctt tgtgcgcctc cattaccgcg tttggcaaaa cacagacgcg
      481 accaagggct tctcgctgat cccctatgtc actctgccta ctggcgatga agataactac
      541 atctcagacg cgaccttcag gggcggactg aaacttgttg gcgactggca gatcaacgac
      601 aaatggtaca cagccataaa cgttggcgca gaattgagag aacccgtgaa ccttctcaac
      661 tacgctgaga agggccgcct tctcgccagc acaggcttgg cctatgcatt caccgagcag
      721 gtcagaggga tggccgacct tgtggcgtca acagccatca acaaaccact caccgaaaag
      781 gtcaccaccc cggttgaagc catgttgggc ctcaacgcca agctcaaaaa gcttcccatc
      841 gcgttcaatc tgagcggcgg gctggccctg attcgcgggg caggcacacc tctgtttaga
      901 actcttgccg gcatgacgat tgggatgtga aataaaggga tgatgttgtg gaagatgggg
      961 atattttgag ttttttgttt aaggtgtgat cacccgcggc aattcgctga tgaaaaacgg
     1021 tggaatgaaa tgaatggttt tgccacgaaa tctttgtgtt cgcatctcgc cgttatatac
     1081 ccaaatgcca cagttcaatc cgcgatgatc cagaaaatcc agcatcggca tgagggaatt
     1141 tttactgaac gacgtttgtg atttgacttc tataggaata attttaccat tcaagggaac
     1201 aagaaaatcg atttcctgac tgttttttgt ttcgcgataa tagttcaaat ccatctgtgg
     1261 ctctggctca agcgccagaa tttcctgaca aacaaatgtt tcctgcaatt ttgcgtagat
     1321 gatattctgc agttctgatc gatcgcgcca agacacaggt tggttttgat gaatcagaaa
     1381 actggtcatc cctacatcaa acaaataccg ctttgaggcg atgccccccg atgattccgg
     1441 gtgtttcgtt tgagccgctg ccttgaacac aagatgccac ttctgcatga tttccaaaaa
     1501 caaaggcacc ttgcgataaa atggagaatc cgttgaaacg atctggctgt tttttaccgg
     1561 ctggcaagcc agatcagcga ttcttcccac gccagacaaa aatagtgagc ggtagttttc
     1621 tttcagatac ctttccacat cagccgcgta ctgcgcgagc agcctgttcc
//
LOCUS       DATDUN010000193         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_465549, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000193 DATDUN010000000
VERSION     DATDUN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465549"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VJC18_02565"
     CDS             complement(<1..370)
                     /locus_tag="VJC18_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLD44294.1"
                     /translation="MKTVLITGASSGIGMALAKELAMRGYHLILMARRLDLMQGWTQF
                     IQTQHPDCQITVIASDVANHEQHMTDVAQAIEKAQGLDLVIANAGVGFNTNEWTNTWE
                     GTKKIFDVNLLGAIATIETAK"
     gene            complement(584..1510)
                     /locus_tag="VJC18_02570"
     CDS             complement(584..1510)
                     /locus_tag="VJC18_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HLD44295.1"
                     /translation="MSGIGVVLNPFSKKYKKNPGKLEHMAFIIGDKASCRPTEDLDDL
                     YRVVEAFKSREIDIVAISGGDGTIHCTLSAFLKIYGEKPLPKVTLLRGGTLNTIAATL
                     GIRGSTEQLMSELLVKYHEDKNFEIRKLRLTKINESYGCIFGMGVIYNFMEEYYKNPE
                     VNPFIAGKTLVASLLSGVVQGKTIKRMFKRFDADVFVDGVKWPFANYSALFTGSIRQL
                     GLEFNVFRHMLTQNEKICAMGISANAGDLMPYLLNLREGKASGSPDVLDAAVDKMEIY
                     LKEPLPYTIDGDMLPAIDKFIITAGPEVSVLV"
     gene            complement(1705..>2401)
                     /locus_tag="VJC18_02575"
     CDS             complement(1705..>2401)
                     /locus_tag="VJC18_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HLD44296.1"
                     /translation="QMSFTLPAPASEEARVAARVLMEKMGFANVRVITMESLGTDFSF
                     FVVYGASPITIDLREIDVPKLEYPQYSFIEINKMLPEKLGRKAIVLGACIGSDAHTVG
                     IDAIFNMKGYLGDYGLERYSAFQAINLRSQVAIDDLVKKIIDMRADAVLISRVVTQRD
                     EHISELKNFLLALQNAQNVPAHLIRVCGGPRITHQEALELGYDAGFGPGTKPSHVASY
                     LATELVRRLDGKK"
BASE COUNT          611 a          642 c          551 g          597 t
ORIGIN      
        1 ccttggccgt ctcaatcgtg gcaatggccc ccaacagatt cacatcaaat atttttttgg
       61 ttccttccca cgtattcgtc cattcattgg tattgaaccc cacgcccgca ttggcaatca
      121 ccagatcgag accctgagcc ttttctatgg cctgcgccac atcagtcatg tgttgctcat
      181 gattggcaac atccgaggca atcacagtga tctgacaatc gggatgttgt gtttgaataa
      241 attgtgtcca accctgcatg aggtctaagc gacgcgccat gaggataagg tgataccccc
      301 gcatagccag ttctttggcc agcgccatgc caatcccgct actggcgcca gtaatgagaa
      361 cagttttcat atggcacaag ctcaaagctc aaaggggaaa aaacgaatat cgaatcatga
      421 acaaggaaca aggaatttcg aagtttttgt cattccagca aaagctggaa tccagaattg
      481 ttggaaacat ggatcccagc gtgcgctggg atgacattct tcgacatttg atattccctg
      541 atcgatattc acttcccttt cagctttcag ctttgagcta ttatcacacc aacaccgaca
      601 cctcaggccc cgcagtgatg atgaatttgt cgatggcggg caacatgtcg ccatcaatgg
      661 tgtacggcaa aggctctttt aaatagattt ccatcttgtc aaccgccgca tccaaaacat
      721 caggagaacc agatgccttg ccttccctga gatttagcag gtagggcatg agatcgccgg
      781 cattggcact gatccccatg gcgcagatct tttcgttctg ggtgagcatg tgtcgaaaga
      841 cgttgaactc cagacccaat tgccggatgg aacccgtgaa cagcgccgaa taattggcaa
      901 acggccattt gacgccatcc acaaaaacat cggcatcaaa acgtttgaac atgcgcttga
      961 tggtcttgcc ctgcaccaca cccgagagca aagaggccac caaagtcttg cccgcgatga
     1021 acggattcac ctcggggttt ttgtagtact cctccatgaa attgtagatc acacccatcc
     1081 caaaaataca gccgtaggat tcgttgattt tggtgagcct gagcttcctg atctcaaaat
     1141 tcttgtcttc gtgatacttg accaaaagct ccgacatgag ttgttcggta ctgcctctaa
     1201 tgcccagcgt ggccgcgatg gtattgagcg tgccgcccct gagcaaggtc actttgggca
     1261 atggtttttc gccataaatc ttgaggaagg ccgacaacgt gcaatgaatc gtgccatccc
     1321 caccagaaat ggccacaata tcgatctcac gggacttgaa ggcctctacc acgcggtaca
     1381 aatcatcgag gtcctccgtc gggcgacaac tggccttgtc accgatgatg aaggccatgt
     1441 gctccagctt gccgggattc tttttatact ttttagaaaa aggatttaag acaacgccaa
     1501 ttccggacat aaataataaa atgaatatcg aatattgaac aaggaatgtc gaatatcgaa
     1561 atttgtcatc cctgcgaagg cagggatcca gaacgaatgc acatatggat tccagcattt
     1621 cagccgttgg actgatcccg tttgcgggac gctggaatga cagccccctt cgatattccc
     1681 tgttccttgt tcaatattcg aaattcattt tttcccatca agtcttctca ccaactccgt
     1741 cgccagataa ctggccacat gcgagggttt ggtaccggga ccaaaaccgg catcgtaccc
     1801 caactccaga gcctcctgat gtgtgatgcg aggcccaccg caaaccctga tcaggtgcgc
     1861 agggacgttt tgggcatttt gcagggccaa tagaaaattt ttcagttcgg aaatgtgttc
     1921 gtcgcgctgt gtgaccacgc gtgagatcag caccgcgtcg gcccgcatat caatgatctt
     1981 tttcaccaga tcatcgatcg ccacctgtga acgcaaattg atggcctgaa aggccgagta
     2041 ccgctccaat ccatagtcac ccagatagcc cttcatgttg aagatggcgt cgatgcccac
     2101 cgtgtgcgcg tcggacccga tgcacgcgcc caaaacgatc gccttgcgtc ccaacttttc
     2161 gggaagcatc ttgtttattt cgatgaaact gtattgcggg tattccagtt tgggaacatc
     2221 aatttctcta aggtcgatcg tgatagggga ggcaccgtaa accacaaaaa aactaaaatc
     2281 ggttcccagg ctttccatcg tgatcactct gacgttggca aatcccattt tttccatgag
     2341 caccctggcc gccactctgg cttcttccga cgctggcgcg ggcaaagtaa aactcatttg
     2401 g
//
LOCUS       DATDUN010000194         1898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_93730, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000194 DATDUN010000000
VERSION     DATDUN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93730"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..559
                     /locus_tag="VJC18_02580"
     CDS             215..559
                     /locus_tag="VJC18_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLD44297.1"
                     /translation="MKYEMTKEQNVAVVKLQGSLDVSLQKELREVLVKITHASEKDLI
                     IDFSSVTFIDSSCLGTLVSLAKNLRERRGDIKLTGLSDDVRSIFQITRLDRVFDIHDS
                     NEEAIAGFYKPQ"
     gene            561..>1898
                     /locus_tag="VJC18_02585"
     CDS             561..>1898
                     /locus_tag="VJC18_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLD44298.1"
                     /translation="MKVSVPFKISSETVIVEVFENSLFKLQRKNTKIYFSSRLCNDLK
                     VAFMEAVTNAIKHAAELTHQRNVQGKFLLDDKIVGFEIYDHGQGFHLDRVPVPDLNDL
                     KASGRGIFMMKQLGECVSYQVHKKRNALVFKRNLIGHHASTRELDLLYELSEAIISNA
                     GREEVYQIILSQALELFHVERASILIFDETDKRLKVAASRGISDQVREHTSIRPGEGV
                     SGYVFQHGRSLLIEDINLNTRGLERKKHYKTSSFISAPMICSPLRLDEKPIGVINLTD
                     RVDGKKFTKKDLKLLSTIANQAMACLHIRNLIEETKEKERLKQEMDHVRRIQSSYLPV
                     QAPAIDGWDLAGRCDMAQSVGGDYFDFHFVSPHLYLIIADVAGHNTSSAVTMVNFRSQ
                     LKAYLSVMSQPQDILARLNQSLYHDLTQAEQFVSTLLIQINVQTGDYVLANAGH"
BASE COUNT          496 a          455 c          498 g          449 t
ORIGIN      
        1 tttcagggca acattacggt ggtggccaaa aaactcaaga tcggccaggc cacgttgtat
       61 cgcaaggtaa aaaaattcgg gttgcaatct tgatctgttt ttttcagggt ccctgaaata
      121 aacacggcgg caacaggacc cgcatcaatg gcatcaggcc attggggcgc gcccgaagat
      181 cgagtgacag atatcagatg aggggagaac gagcatgaaa tatgaaatga caaaagaaca
      241 gaacgtggcc gtggtcaaat tgcaggggtc cctggatgtg tccctgcaga aagaattgag
      301 agaggtgctg gtcaagatca cgcacgcctc cgaaaaagac ctgatcatcg atttttcctc
      361 tgtcacgttc attgacagtt cgtgtctggg gaccctggtt tcactggcca aaaatttgag
      421 ggagcgaaga ggtgatatca agttgacggg cctttccgac gatgtgagga gtattttcca
      481 gatcacgagg ctcgatcgtg tttttgatat tcacgacagc aacgaagaag cgatcgccgg
      541 tttttataag ccacaatgat atgaaagtca gtgtcccttt caaaatatcc tcagaaacgg
      601 tcattgtcga agtttttgag aactcgctct tcaaactcca gcgaaaaaac accaaaattt
      661 atttttcgag tcgcctgtgc aatgacctga aggttgcttt catggaggcc gtgaccaatg
      721 ccatcaaaca cgcggctgaa ctcactcatc agagaaatgt gcaggggaaa tttctgctgg
      781 atgacaagat tgtgggtttt gaaatctacg atcatggcca gggttttcat ctcgaccgcg
      841 ttccggtacc ggacctgaat gacctcaagg cctctggtcg aggcattttt atgatgaaac
      901 agttgggcga gtgtgtctct tatcaggtgc acaaaaagag gaatgccctt gtgttcaaaa
      961 gaaacctgat cgggcaccac gccagcacac gggaactgga tttgttgtac gagttgagtg
     1021 aggccatcat cagcaacgct ggccgcgaag aggtttatca gatcatcttg agtcaggctt
     1081 tggagctgtt tcacgtcgaa cgggcctcga ttcttatttt tgatgaaacc gataaacggc
     1141 tcaaagtcgc ggccagccgg gggatttcgg accaggtgcg cgagcacacg tcgattcgcc
     1201 cgggggaagg cgtcagtggt tacgtgtttc agcacggacg gtctctcctc atcgaggaca
     1261 tcaacctgaa cacccgcggg ctcgaacgca agaaacacta caagacctca tccttcattt
     1321 cggccccgat gatttgttcc ccgctccgtt tggacgaaaa accaatcggg gtgatcaatc
     1381 tcacggatcg cgtggatgga aaaaaattca ccaaaaaaga cctcaagttg ttgtccacga
     1441 tcgccaatca ggccatggcc tgtctgcaca tccgtaatct gatcgaagag accaaggaaa
     1501 aggaacgact gaaacaggaa atggatcacg tgcgtcggat tcaaagttct tacttgccgg
     1561 tgcaagcccc tgccatagat ggctgggatc tggcggggcg ctgtgatatg gcccaatcag
     1621 tggggggaga ctattttgat tttcattttg tctctcctca cctgtacctc atcattgccg
     1681 atgtcgctgg ccacaacacg agcagtgccg tgacgatggt gaattttcgt tcgcaactca
     1741 aggcttacct gagcgtgatg tcacagccgc aggatattct ggccagactc aatcagtctc
     1801 tctatcacga tttgacccaa gcagaacagt ttgtgtcgac tctgctcatc caaatcaacg
     1861 tgcagacggg ggactacgtt ttggcgaacg ccgggcac
//
LOCUS       DATDUN010000195         1777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_383563, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000195 DATDUN010000000
VERSION     DATDUN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383563"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..>1777)
                     /locus_tag="VJC18_02590"
     CDS             complement(230..>1777)
                     /locus_tag="VJC18_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44299.1"
                     /translation="DSLSGTTLSFQVAVTDGTDAATDHISIEVVSNEISSADALLLHE
                     HVVGVDGGDEVISEKLEEMDAVEVSQLKLWNDCVIVATTDNPVTLLNIDARHLALGDP
                     YADDETGTVIILDRDRLDCSTPIDFADEDLSGRTGVKVISGNQAGDRFGTTMKLGDID
                     GDDVFEIYVVAPGNGNGYVYGLNGVTLSIERLFIGAADKPLTSTLMEFGNFDGISGSE
                     CLFGLSVALGSDDNQTAVEDLTSLSVSAVSGFAGFSGTDAFPAVVNMAATESDYDLSS
                     SIGISHLTKVDLDADAREDVLFGSGEACEIFILYGSDSFGAGDASVDTQVKVQCSEDT
                     TFSTLVTSQDVTGDGFVDVIAGFPEAGDGAGRLLVLFGPLDPEQETYDMDHALVIEGV
                     AGENLGTEVVVGDYDLDGIADLFATVLVGGEKKTKVLMLSSLLEGNSYATGGDSVSGG
                     SGGGCSLQISNAAKTHEDWFMALNLFLVMLLSCVHRWRLESLQRNGIRRACHTIRIMP
                     NYGIIFK"
BASE COUNT          485 a          517 c          349 g          426 t
ORIGIN      
        1 ccgaataggg caaattccct atgtataact tcttactcat gtattgtctc cttgatttaa
       61 tgcttttctt aagggctgga taatacaacg gacggtcaaa attgacgcta ttgacttccg
      121 gtctcttaga aaaccgccaa tagtataaca gcattttaac tcaagcgcaa tatgaatcgg
      181 tttttttgac acatctccta actatttatt attttttatg tttttgatgc tatttgaaga
      241 ttattccata atttggcatg attctgattg tgtgacacgc cctgcggatg ccatttcgtt
      301 gcaatgattc cagccgccac ctgtgcacac aactcaacaa catcaccaaa aacagattca
      361 aggccataaa ccagtcttca tgtgttttgg ccgcatttga tatctgcaat gaacaacctc
      421 cgcctgatcc tccagacaca gagtcaccac cggtggcata actgttacct tcgagcagtg
      481 atgacagcat gagcaccttg gtttttttct ccccacccac aaggaccgtg gcaaacaaat
      541 cggcgatgcc atccagatca taatcaccaa caacaacctc tgttcccaga ttttccccgg
      601 caacaccctc aatgaccaac gcgtgatcca tatcataggt ctcctgttct ggatcaagag
      661 ggccaaacaa aaccagcaag cgaccagccc cgtcaccggc ctccggaaat cccgcgatca
      721 cgtcaacaaa cccgtcacca gtcacatctt gagacgtcac cagggtagag aacgtggtgt
      781 cctcggaaca ctgcactttg acctgcgtat ccacactcgc gtcaccagca ccgaacgagt
      841 cacttccgta caaaataaat atttcacagg cctcaccaga cccgaagagg acatcttcgc
      901 gcgcgtcagc gtcgaggtca accttcgtta aatgagatat gccaatcgag gaagatagat
      961 cgtagtccga ttcagtcgcc gccatgttga caacagccgg aaaggcatcc gtaccactga
     1021 atccagcaaa accactcacg gcactcacgc ttaatgaagt taaatcttca actgccgttt
     1081 ggttgtcatc actgcccaag gccacgctca gaccaaaaag acattcggaa ccagaaatgc
     1141 catcgaaatt tccaaattcc atcaaggttg acgtcagggg tttatcggca gcgccaatga
     1201 acagcctctc aatgctcagc gtcacaccat taagtccgta cacgtaccca ttaccattgc
     1261 cgggtgccac tacgtaaatt tcaaatacat catcaccatc aatgtctcca agcttcatgg
     1321 tcgtcccaaa ccggtcaccc gcctggttgc ccgaaatcac cttcacccca gtcctgccag
     1381 acaggtcctc gtcagcaaaa tcaatgggag tcgaacaatc caaccgatcc ctgtcgagaa
     1441 tgatgacagt ccccgtctcg tcatcggcat agggatcgcc cagcgccagg tgacgcgcgt
     1501 caatattgag tagggtgacc ggattgtcag tcgtggcgac tatgacacag tcgttccata
     1561 atttgagttg actcacctcc acagcatcca tttcctcaag tttttctgaa atcacctcat
     1621 ctccaccatc cactccaaca acatgttcat gcaacaatag ggcgtctgcc gatgatattt
     1681 cgttcgacac aacttcgatg gaaatatgat cggtggccgc atcggtgcca tcagtcacgg
     1741 caacctggaa cgaaagagtc gtcccgctca ggctgtc
//
LOCUS       DATDUN010000196         1718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_216082, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000196 DATDUN010000000
VERSION     DATDUN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216082"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1326
                     /locus_tag="VJC18_02595"
     CDS             319..1326
                     /locus_tag="VJC18_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HLD44300.1"
                     /translation="MVDINNFSFSPERFRTEGGVSPNDTGDCLEDILAQRETTPSLNL
                     PSITKACPNPLWRPEFSCELARNSTDSSPLPWVFADTSHDTSTPVNFFLTDLETDLDS
                     LPYDATQFQKVTLIFNAVRLLEWELSQSPSGQTCTELISNPVGNCVDMTNLMYAICRH
                     FEIECSLIYVYENFKSAINHVCLGIKDDRGETILIDATVADGMDIGYPAWYEISECEF
                     LAMHHTARGVRLEDDLSDDEALKEYGLSRAVYPPGPIPLLNSIGIYMRQGESALAGQG
                     LADLSSKGYNSAQFQYLLGHYYLHNHHLRLAQQAFENAVHLCPQHEGAREQLEQLTSR
                     Q"
     gene            complement(1331..>1718)
                     /locus_tag="VJC18_02600"
     CDS             complement(1331..>1718)
                     /locus_tag="VJC18_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HLD44301.1"
                     /translation="RVALGIGRTGSYAAHGSGEIILAFTTANKIPRQRKKEIIDMKVM
                     PDDLLDSFYRAVIEATEEAILNALCMANDMSGIYRRFAPALPLDDVRQVLNTYQIPQT
                     KIKKADLVDKSDQTSIMQQVGTILKK"
BASE COUNT          459 a          434 c          375 g          450 t
ORIGIN      
        1 tttattttga tcaaacggta tttgaaacaa tctcgaacct tgattttgga aaccccgatc
       61 tctttgtcct cgatgaggtc ggtcccctag aattggaaga caaactggga ttttggcctt
      121 tcttagaaaa cgtctaccaa acccacaatc acactatcac gatcgtcagg gaaaactgcc
      181 tcgatctctt tcgcaatacg tttgcaacac atcactttga tgcaataatt tactcaatat
      241 attcatagag ataacttttc tctcagtttt ggcgaaactt ttttgtcaag tcaccgataa
      301 tggcggcgga gtcaatgcat ggtcgacatc aacaacttct cattttcccc agaaaggttc
      361 agaactgaag gaggggtgag tcccaacgac acaggcgatt gccttgagga tatattggcg
      421 caaagggaga caacaccatc attgaacttg ccatcaataa caaaagcctg tcccaacccg
      481 ttatggcggc ctgagttcag ctgtgaactg gcccgcaaca gcactgattc aagtcctctc
      541 ccttgggtgt ttgctgacac aagccatgac accagcaccc cagtaaactt ttttctgact
      601 gatctcgaaa cggacctgga ttcactccct tatgatgcca cacaattcca aaaagtgact
      661 ttgattttca atgctgtgcg gttactggaa tgggaactga gccagtcccc gtccggacaa
      721 acctgcaccg agcttatcag caaccctgtc ggaaattgcg tggacatgac caatctcatg
      781 tatgcaattt gccgtcattt cgaaattgag tgttccctca tttatgtcta cgagaatttc
      841 aagagtgcca taaaccatgt gtgcctcgga atcaaagatg accgaggtga aacaatcctc
      901 atcgatgcca cagtggcaga cggcatggac attggttatc ccgcctggta cgaaatttcg
      961 gaatgtgaat ttttggccat gcaccacaca gccaggggag tgaggttgga agatgatttg
     1021 agcgatgatg aggccctcaa agaatatggg ctctctcggg ccgtttaccc accaggcccc
     1081 attcccctgc tcaacagcat tggcatttac atgagacaag gtgaaagtgc tttggctgga
     1141 caaggcctgg ccgacctctc ctcaaaaggt tacaactctg cgcaatttca atacctgctc
     1201 ggtcactatt atttgcacaa tcatcattta agactcgcac agcaagcatt tgaaaacgct
     1261 gtccatcttt gcccacagca cgaaggggca agagaacaat tagagcaact cacatcgaga
     1321 caataaaaat ctattttttc aatatcgttc ccacctgttg catgatgctc gtctggtcgg
     1381 atttatcgac gagatcggcc tttttgattt tggtttgtgg aatttgatag gtattcaaga
     1441 cctgcctcac atcatccaaa ggaagcgcag gagcgaaccg cctatagata ccactcatgt
     1501 cgttcgccat acacagggcg ttcaggatgg cttcctctgt ggcctcaatg acagcacggt
     1561 aaaaggaatc cagcaaatcg tctggcatga ccttcatgtc gatgatttct ttttttctct
     1621 ggcgtggtat tttgttggcc gtggtgaagg ccagaatgat ctcaccagaa ccgtgggccg
     1681 cgtacgaacc cgtacgacca atccccaagg ccactctt
//
LOCUS       DATDUN010000197         3021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_548529, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000197 DATDUN010000000
VERSION     DATDUN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548529"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..535)
                     /locus_tag="VJC18_02605"
     CDS             complement(263..535)
                     /locus_tag="VJC18_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HLD44302.1"
                     /translation="MEQVKTNPLKCIYGNTVKLQNTKTRVVELYALVPPNQERLDKKH
                     ISNYTDVGQAIWGKMENDVVEIELPGKGKELFKILGVTSTEVGGRQ"
     gene            562..1926
                     /locus_tag="VJC18_02610"
     CDS             562..1926
                     /locus_tag="VJC18_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD44303.1"
                     /translation="MFQSKKSNAVLVLMGLFLCLLVACEKSDSAKVDMLSLPTPVDPI
                     KTTDKPLTGQPQSPVGSTEIKPPVINVVTSCQDSIKNDSAIEVFREVSPSEISLTINS
                     NKIIDNDSTEKWLKDFVYDLFDRQQCPYKTIAFTTIVDEYSYLQGLITRLDFDLFKQQ
                     KISEPEWIRRFDIKKMTTTEAVRASLRKARQLGDSGTALELVEKLLLDDYASTALKII
                     KANILVEQKQYVEAIDIYRDVILQEPFNVNAWFNLSYCQKMAGQFAEAVENAKKLLAF
                     ITEPAKQEVYLKALGLTRDDLWLHLAECYSKNNEIILAQEALNKVVTKNNPVYGLLTA
                     NLLRLDHKAEQARTVLLSLLDTGQRQDLVFYNLVVLSLDLKDEKGAQDYYQMLRENHP
                     NEARELAFVMNLPKHVPAETDTPLVVSTPVTEEQLAPEVNLPPAAIMPVVGDQGTKEQ
                     RTGE"
     gene            1929..2210
                     /locus_tag="VJC18_02615"
     CDS             1929..2210
                     /locus_tag="VJC18_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44304.1"
                     /translation="MRLFVAFISVLIVACAFHARAEGPALPTEDVVKQTRVENVEALE
                     LPYQPAQNVENADGTEEPLDPTQPVIEWSKVPYVPSHYPVVGPARDPEI"
     gene            2250..>3021
                     /locus_tag="VJC18_02620"
     CDS             2250..>3021
                     /locus_tag="VJC18_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLD44305.1"
                     /translation="MGEEPKQRILSVIKARYPHLSQKNIRELLSQKKISVGKRVAKVN
                     DCVGESDLIVMPDECLRRELVANPRVDCRLIKKTKEYIFFEKKERVHSVAQNYVETAS
                     AANWLLSVDGMLSIIGKPLEAGLCHRLDYETSGVMVAARNQSAYEHLRKLFATGLVHK
                     IYVCLVSEPPPKSGQYTAQVLPGRKAARKVTLQACVSDRGVVVETEILASEKTHDGWY
                     RITLSLITGFRHQIRAHMALLGCPLIGDALYGGRKAERL"
BASE COUNT          823 a          652 c          767 g          779 t
ORIGIN      
        1 cggcatggcg ggcaaggtga tgacgaattt ggaacaggtg tggggcaggg ggattgatac
       61 agaggaatat tcacgccttt ctgccagctt gggacaaaga ctcggtgaat caatgaaaca
      121 atgaatcaat gaaacaatga atcaatgaaa caatgaaaca ataaatcaat gaaataatgg
      181 attaatcatt aatatcattg attgattgat tgattgattt attcattgat tgattcattc
      241 attgtttcat tgtttcattg tttcattgcc tcccgcccac ctcagtgctt gtcaccccca
      301 atatcttgaa cagctccttg cccttgccgg gtagttcgat ctccacaaca tcattttcca
      361 ttttgcccca gatggcttgg cccacgtcgg tgtaattgga aatgtgtttt ttatcgagtc
      421 gttcctgatt cggggggacc agggcataaa gctcgacgac tctggttttg gtgttttgaa
      481 gtttgacggt gttgccgtaa atgcatttga ggggattggt tttgacttgt tccatcactg
      541 ttgtgtatag ttaacgaagc tgtgtttcaa tcaaaaaaat caaatgccgt ccttgtgttg
      601 atggggttgt tcttgtgtct tttggtggcc tgtgaaaaat cagattccgc gaaagtcgat
      661 atgctgtccc tgccgacacc agtcgatccg ataaaaacca cggataaacc gttgacaggt
      721 caacctcagt cgcccgtcgg gtcaacggag atcaaaccac ctgtgatcaa tgtcgtgaca
      781 agttgtcagg attcgatcaa aaatgattca gccattgaag ttttcagaga ggtcagtcct
      841 tctgaaatct ctctcacgat caactcgaac aaaatcattg ataacgactc aacagaaaaa
      901 tggcttaagg atttcgtcta cgatttattt gatcgtcaac aatgtcctta taaaaccatc
      961 gcttttacga cgatcgttga tgagtattct tatttgcagg gtctcatcac caggctagac
     1021 ttcgatttgt tcaagcaaca gaagatttcc gaaccagaat ggatccggcg tttcgacatc
     1081 aaaaaaatga ccacgacaga ggctgtccgc gcgagcctgc gcaaagccag acaattgggg
     1141 gattccggga cggcgctgga attggtcgaa aaacttctgc tggatgatta tgcgagcaca
     1201 gccctgaaaa tcatcaaagc aaatattctg gttgagcaga agcagtatgt tgaggctatc
     1261 gacatctatc gtgatgtgat cttacaggaa ccctttaatg tgaacgcctg gttcaatttg
     1321 tcctactgtc aaaagatggc tgggcagttc gcagaggctg tcgaaaacgc aaaaaaattg
     1381 ttggccttca ttacggaacc ggcaaaacaa gaggtctatc tcaaggcgtt ggggttgacg
     1441 agggatgacc tgtggttgca tttggccgag tgttattcca agaacaacga aattattctc
     1501 gcccaggagg ccctgaacaa agttgtgacc aagaacaatc ctgtttatgg tctgctcacg
     1561 gcgaatcttt tgcgactcga tcacaaggcg gaacaagcca ggaccgtttt gctatcactg
     1621 ctcgacacgg gacagagaca agacctggtg ttttacaatt tggtggtgct ttcgttggac
     1681 ctcaaagatg agaaaggggc ccaggactat tatcagatgc tcagagagaa tcaccccaac
     1741 gaggcacgtg agttggcatt cgtgatgaat ttgcccaaac atgttccggc cgaaacggac
     1801 acaccgctag tggtctcgac acctgtcact gaagaacagc ttgcgccaga agtgaacctt
     1861 cccccagcgg caatcatgcc ggtcgttgga gatcagggga caaaagagca gaggacggga
     1921 gaatgatgat gcgtttgttt gttgcattta tttctgtgct gattgtggct tgtgcttttc
     1981 atgcacgtgc tgagggtcct gccttgccca ctgaagatgt ggtgaaacaa accagggttg
     2041 agaatgtcga agcactggaa ttgccctatc aaccggcgca gaacgtagaa aatgccgatg
     2101 ggactgaaga accactggac ccaacgcaac cggtcatcga atggtcaaaa gttccctatg
     2161 tgccatcaca ttatcccgtt gtgggaccgg ccagggatcc ggaaatataa ggaatcaatc
     2221 aatcaatgaa gtaatgaagt aatgaagtaa tgggagaaga acctaaacaa cgcatccttt
     2281 ctgtcatcaa ggcccgttac ccgcaccttt cacaaaaaaa tatccgtgag ttgttgtcgc
     2341 aaaaaaaaat tagtgtgggc aagcgcgtag ccaaagttaa tgactgtgtg ggtgagagcg
     2401 atctcatagt catgcccgac gaatgtttaa ggcgtgagct tgttgccaac ccgcgagtgg
     2461 attgtcgact catcaaaaaa acaaaagagt atattttttt cgaaaaaaaa gagcgggttc
     2521 attccgtggc gcaaaactat gttgagacag cctcggccgc caactggctt ttgagtgttg
     2581 acgggatgtt gagcataatt ggcaaaccgt tggaagcggg attgtgtcat cggcttgatt
     2641 acgagacgag tggtgtgatg gtggccgctc gcaatcaatc agcatatgag catctcagaa
     2701 agctgtttgc aacgggtctg gttcataaaa tttatgtctg tcttgtttcc gaaccacctc
     2761 ccaagtcagg tcaatacact gcgcaggttt tgccaggcag aaaagcggcc aggaaggtga
     2821 cgttgcaagc gtgcgtgtcg gaccggggtg ttgtggttga aacagaaatt ctggcgagtg
     2881 aaaaaactca cgacggatgg tatcggatca ctttaagcct catcacgggt tttcgccatc
     2941 aaatccgtgc ccatatggca ttgctcggtt gtcccctgat cggtgacgcg ttgtacgggg
     3001 gcagaaaggc cgagcgtctc a
//
LOCUS       DATDUN010000198         1187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_216256, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000198 DATDUN010000000
VERSION     DATDUN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216256"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VJC18_02625"
     CDS             complement(<1..343)
                     /locus_tag="VJC18_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD44306.1"
                     /translation="MISKTGINLFTYLDYRKYLKDWYEYHKQNTTGFSFRSFSQKAGF
                     GSPNFLKRVIEGERNLTESSTRAVIKCLELNKQESAFFERLVLFNQSTSHEEKNVHYH
                     SIVTSQKFAQLK"
     gene            complement(579..>1187)
                     /locus_tag="VJC18_02630"
     CDS             complement(579..>1187)
                     /locus_tag="VJC18_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44307.1"
                     /translation="ASPVPTRRPLVVMPKEPLVPTPPPPRMTREERIAEYTRLRAMMA
                     PANDGPEEALEDDETGEEEIVGVVARHASRPLEVDPSTMIASHVERYRWLDTPSLRVL
                     LRARDTPIEILDEGGLDKIRGTVHHDALLGALAKLKFAPSLLRHQIKKVNHRRSKAPT
                     KYLSLKITHKLRMVYSHETGRIVVAFIGYHEDYETFLDRLSL"
BASE COUNT          284 a          298 c          274 g          331 t
ORIGIN      
        1 gtttgagttg cgcaaatttc tgcgaggtca caatagaatg gtaatggaca tttttttctt
       61 cgtgtgaggt gctctggtta aacaatacca gcctttcaaa aaaagcactc tcctgtttgt
      121 tgagttccag acatttgatg acggcccgcg tactgctctc ggtcagatta cgctcccctt
      181 cgatcacccg tttgagaaaa ttgggagaac caaaccctgc cttttgcgaa aacgatctga
      241 atgagaatcc tgtggtgttt tgtttgtggt actcgtacca gtccttcagg tattttcggt
      301 aatcgagata ggtgaaaaga tttatgccag tttttgaaat cataatatta gtgtgtacag
      361 tgacttgtca ttccagcgaa agctggaatc catgtttccc acaatcctgg atccctgcct
      421 tcgcagggat gacacaacca acatctgcca tctacatcta catctacatc tgcctcaccc
      481 gtatccaaat ctcttccgtt ctgtctatga tattacagtt atttgattta ttatcaatag
      541 gttcagtatt tatttgtatc ctaatggata cgtgaaaatc acagagacaa ccgatccagg
      601 aaggtttcgt agtcctcgtg gtagccgatg aacgccacca caatgcgacc tgtctcgtgc
      661 gaatacacca tgcgcagttt gtgggttatc ttcagagaca agtacttggt gggagccttg
      721 gaacgacgat ggttcacttt tttgatctga tgacgcaata gactcggtgc gaatttaagt
      781 ttggccaagg cccccaacaa ggcgtcatga tgaactgtac cgcgaatctt gtccagccca
      841 ccctcatcca ggatttcaat aggagtgtcc ctcgccctca ataagactcg caaggaaggt
      901 gtgtccaacc accgataccg ctccacatga gaagcaatca tggttgaggg atcgacctca
      961 agaggtcgtg aagcgtgtcg tgcgaccacc cccacgattt cttcttcacc agtctcatca
     1021 tcttccaacg cctcctctgg cccatcattc gcaggcgcca tcatggccct gagtctggtg
     1081 tattcagcga ttcgttcctc tctggtcatg cgtggcggtg gcggcgtggg caccagtggt
     1141 tcttttggca tgacaaccaa cggtcgccgt gtgggaacag gagaggc
//
LOCUS       DATDUN010000199         3008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_134424, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000199 DATDUN010000000
VERSION     DATDUN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134424"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="VJC18_02635"
     CDS             <1..1974
                     /locus_tag="VJC18_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD44308.1"
                     /translation="ACDATKLEAITKLFPTEETRATHINIKPYTLEETDRFLTHIIGQ
                     KEIPKKFVEQFYLNTEGLPGIALDLIQSMIANGLLFDKSGRWNEDLLVNLESTFDSIQ
                     VSETLEQEFEKTYNSLTGTEEDIVNWLSLCPHPLTGANLISLIGQTGVDSALQTLLHK
                     NIVRNENGQITLARSVFQNFIQNNLPNVDAQRRHTLLAHPKTKLDKFWALYHLSLGNN
                     PDLKMKAGSKLAETYKNRGERDKAVQCYLNLIKDNATLPLSAKIDWYIEAASLLIWLD
                     RFVQTVEIISGLEQEIQKHKTPVQFEKFLILLEKKGQALLHQLQLEKAKTYFDTGLKH
                     AVKSDKYKVQELRFQNDLGLIETILGHKNEAIAIFKKTRDAAKQLPPEKQPDITNNDL
                     GHVYLSLKQSEDATKYLTEDIELFTPLTNKEPLARALYSFAELLAGQKNFDGAIAASE
                     KCAKLCRDSNNHPLLLRVYNGLGNTYLLLEDFEQAIKNYQKAIEIAVRLNELTSKAAL
                     LYNQGVIYRKDKNSALALRRFLMAKQVLENKKGELLAYDESLLSRCYADLAQLSIDEQ
                     NSLKALSYQLERIKLVTSSENLKPELFSAKYDLAKIYMENRLNDQFTTEIEELRKIAA
                     SDEDKEAVTKLSKQWETIQNHTTQEITGKIVLK"
     gene            complement(1978..2628)
                     /locus_tag="VJC18_02640"
     CDS             complement(1978..2628)
                     /locus_tag="VJC18_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HLD44309.1"
                     /translation="MQRQCVIFDMDGVLVDSGPWHLKAWQVFANKYGLTFDEAHYFST
                     FGMRNDEIIPKLFPKQFNPKDFYALNEEKEAFYRDLIRGKIVPPAGLMAFVADLRQQG
                     IRMAVGSSGTRPNVLLVLEALRLTDLISAYVCGDDVKRGKPAPDVFLLAAQKMGVAPQ
                     FCVVIEDAVMGIKAAKTAGMQCVAITTTTTREHLHEADMIVDSFTELSAQTVRDLL"
     gene            2674..>3008
                     /locus_tag="VJC18_02645"
     CDS             2674..>3008
                     /locus_tag="VJC18_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44310.1"
                     /translation="MHILASIGFLLVFVSALIFMHVYPMQKLLGLSAEALTQAGLAVP
                     THKALSLSFSLCLVFLVMLAFMLGTFFPKGVLGTLHKISSFWLGALFIFFFVVLCVDL
                     IGRFLPITP"
BASE COUNT          820 a          874 c          674 g          640 t
ORIGIN      
        1 gcctgtgacg caacaaaact cgaggccatc accaaactgt tcccgacgga agaaacccgc
       61 gccacgcaca ttaacatcaa gccctacacg ctggaagaga ctgaccggtt tctgactcac
      121 atcatcggac aaaaggaaat ccccaaaaaa tttgtggagc agttctatct caacaccgag
      181 ggtctgccag gcatcgcgct ggatctgatt caatccatga tcgccaatgg cctgctgttc
      241 gacaagagcg gccgatggaa cgaggacctg ctggtcaacc tggagagcac gttcgattcc
      301 attcaggtgt ctgaaacact ggaacaggaa ttcgaaaaaa catacaactc cttaaccggt
      361 accgaggaag acattgtcaa ctggctgagc ctgtgtccgc accccctcac cggggccaac
      421 ctgatctcac tcattggcca gacaggcgtt gattcggccc tgcagacgct cctgcacaag
      481 aacattgtcc gcaacgaaaa cggacaaatc actctcgcac gcagcgtgtt tcagaatttc
      541 attcaaaata atctccccaa tgtcgacgca caaaggagac acaccctgct ggcccacccc
      601 aaaaccaaac tcgataaatt ctgggcgctc tatcacctga gtttgggcaa caaccccgat
      661 ctcaaaatga aagcgggtag caaactggcc gagacctaca aaaaccgcgg tgaacgcgat
      721 aaggccgtgc aatgttactt gaacctcatc aaggacaacg caaccctccc cctctcagcg
      781 aaaatcgatt ggtacattga agccgcctct ctgctgatct ggctggaccg gttcgtgcaa
      841 actgtcgaga tcatcagtgg tctcgaacag gagattcaaa aacacaaaac tcccgtgcaa
      901 ttcgaaaaat tccttatttt gctcgaaaaa aagggacaag ccctcctgca ccaactccag
      961 ctcgaaaagg caaagaccta ttttgatacc ggtctgaaac acgccgtcaa atccgacaag
     1021 tacaaggtgc aggaactgag gtttcaaaac gatctggggc taatcgaaac cattctgggt
     1081 cacaagaatg aggccattgc catcttcaaa aaaacaagag acgccgccaa acaactcccc
     1141 ccggagaaac aacccgacat caccaacaac gatctgggac atgtttattt gtcactcaag
     1201 caatccgagg acgccaccaa ataccttact gaagacattg agctgttcac gccactcacc
     1261 aacaaggaac cgctggccag agccctgtac tcattcgctg aactgttagc cggtcaaaag
     1321 aatttcgatg gggccatcgc ggcttccgaa aaatgcgcga agctctgccg cgacagcaac
     1381 aaccaccccc tgctcctgcg cgtctacaac ggtctgggca atacctatct cctgctcgaa
     1441 gattttgagc aggcgatcaa aaactatcag aaggccatcg agatcgcggt acggctcaat
     1501 gaactcacgt caaaagcggc cctgttgtac aatcagggcg tgatttaccg caaggacaag
     1561 aactctgctc tggcgctcag acgtttcctc atggccaaac aggttttgga aaacaaaaag
     1621 ggtgaactcc tcgcctacga tgaatccctg ctctcccgtt gttacgctga tctggcgcaa
     1681 ctctcgatcg acgagcaaaa cagcctcaag gcgctgagct atcagttgga aagaatcaag
     1741 ttggtcacca gttcagaaaa tctcaaacca gaactctttt cggcaaaata tgatctggcc
     1801 aaaatctaca tggagaaccg cctgaacgat cagttcacca ccgagattga ggaactgcgc
     1861 aaaatcgccg cgtcggatga ggataaggaa gccgtcacca aactgtccaa acagtgggaa
     1921 accatccaaa accacaccac tcaggaaatc acggggaaga ttgtgttgaa gtaacaatca
     1981 caacaaatcc cgaaccgttt gtgccgacag ttccgtaaag gaatctacaa tcatatccgc
     2041 ttcgtgcaaa tgctcacgcg tggtcgtggt ggtgatggcc acacactgca tgccggcagt
     2101 cttggcggcc ttgatcccca tcacagcatc ttcgatgacc acacagaact gcggggccac
     2161 ccccattttc tgggcggcca gcaaaaacac atcaggggcc ggtttgccac gcttgacatc
     2221 gtcgccacaa acataggcgc tgatcagatc ggtcaaacgc aacgcctcca aaaccagcaa
     2281 cacatttggc cgggtaccgg acgagccaac agccatcctg atgccctgtt gccgcagatc
     2341 agccacaaac gccatcaaac cggcaggagg gacaatcttc cccctgatga ggtcacggta
     2401 gaaggcctct ttttcctcat tcagcgcgta aaagtctttt gggttgaatt gctttggaaa
     2461 gagtttgggg atgatctcgt cgttgcgcat gccaaacgta ctgaaataat gtgcctcgtc
     2521 aaaagtcaga ccgtatttat tggcaaacac ctgccaggcc ttcagatgcc acgggccgga
     2581 atccaccaac accccatcca tatcaaaaat cacacattgt cgttgcatgt ttgatctcta
     2641 tttctctttg tggcacaagg gaagaaataa tttatgcaca ttctggcttc aataggcttc
     2701 ctcctcgtct ttgtctccgc actgattttc atgcacgtct atcccatgca gaaacttctg
     2761 ggcctttccg ccgaagcctt gacgcaggcg ggcctggcgg ttcccacaca caaggccctg
     2821 tcgctctctt tctccctctg tctcgtgttt ctggtcatgc tggccttcat gctggggacc
     2881 ttctttccca aaggagtgtt gggcacgctc cacaagatca gctcgttctg gctgggcgcc
     2941 ctcttcatct tttttttcgt cgtgttgtgc gtggatctca tcggcaggtt tctgccaatc
     3001 acaccctt
//
LOCUS       DATDUN010000200         1239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_50745, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000200 DATDUN010000000
VERSION     DATDUN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50745"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VJC18_02650"
     CDS             complement(<1..1126)
                     /locus_tag="VJC18_02650"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HLD44311.1"
                     /translation="MSQDSIKIAVVGSGPAGLMCAQVLSHDNSFRVTVFDQYRMFGRK
                     LLIAGKTGLNITNTLSSEAVLQNLTGPHEHFQNVFSSFSKQDWIAFVNSLGVHTYEGS
                     SHRVLIKETATDLLESWITSLKNAGILFTNTHQFIDFSIKENKIDLAFLTTTEANFDA
                     VCLALGGQSYAETDPVWIDRFKEKQISISPLLPSNVGYCVDWSKAFLLEAEGQPLKNI
                     NLKTSKGSLRGELTITQYGLEGTPIYQLGAVGIAHLDLKPDLTKKDLVTKCQKNPKKL
                     SPLRLLVKNLNLSPVAEALLYHHTSPEQKSDLSQMIGLIKNFPVTLTAPRPLAEAIST
                     AGGVCFENLNEGLMLKRFPGVFLAGEMLDWDAPTGGFLIQT"
BASE COUNT          304 a          261 c          329 g          345 t
ORIGIN      
        1 aggtctgtat caaaaaaccg cccgtggggg catcccaatc cagcatctct ccggccagaa
       61 aaacaccggg gaatcttttg agcatcaagc cttcatttaa gttttcaaaa cacacaccac
      121 cggctgtgga gatggcttcg gccaagggtc tgggcgcggt gagcgtgacg ggaaaatttt
      181 tgatcagccc tatcatttgt gacaaatcac ttttttgttc cgggctggtg tgatgataga
      241 gcagggcctc ggcgacaggg ctgaggttga ggtttttgac gagcaatctc aagggagaga
      301 gtttttttgg attcttctga cattttgtca caagatcctt ttttgtcagg tccggtttga
      361 ggtcaagatg cgctatccca accgcaccca attggtagat gggtgtgcct tccaagccat
      421 attgggtaat cgttaactcc ccgcgcaggg agcctttgct ggttttgagg ttgatgttct
      481 tgagtggttg cccttcggct tcgagcaaaa aagcctttga ccagtccaca cagtaaccca
      541 cgttggaggg caagagaggg ctgatggaga tttgtttttc cttgaagcga tcaatccaga
      601 cagggtctgt ctctgcgtag ctctgccccc ccaaggccag acaaacagcg tcgaagttgg
      661 cttcggttgt tgtcaagaaa gcaagatcaa ttttattttc ctttatagag aaatcgataa
      721 attggtgcgt gttggtaaaa agaatgcccg cgtttttgag ggaggttatc cagctttcga
      781 gcaggtctgt cgctgtttct ttgatgagga cgcggtgtga ggatccttcg tacgtgtgga
      841 cacccaatga gttcacaaag gcgatccagt cttgtttgga aaatgaggaa aacacatttt
      901 gaaaatgctc atgaggtccc gttaggtttt ggagaacagc ttctgatgat aaggtgttgg
      961 tgatgttgag gcccgttttg ccggcaatca acagtttacg accgaacatt cggtactggt
     1021 caaagaccgt gacgcgaaaa ctgttatcat gactgagcac ctgtgcgcac atcagaccgg
     1081 ctgggcccga tccgacaaca gcgattttta ttgagtcctg tgacatggtc actgatgctc
     1141 taacatgtct ttgtgtcggt gtgaaaggta aaaaagatca atgttcttga gaaagaacag
     1201 aaaatacttt accatgaata tctgtttttt ttgaaaggt
//
LOCUS       DATDUN010000201         1028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_174879, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000201 DATDUN010000000
VERSION     DATDUN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174879"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            828..>1028
                     /locus_tag="VJC18_02655"
     CDS             828..>1028
                     /locus_tag="VJC18_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44312.1"
                     /translation="MNNSTQPKYLKIILATVIAFYSVACDQNIVAPVDLTVDTSISIA
                     TFTVTDNIIEQETTVTLNWNVTD"
BASE COUNT          280 a          218 c          247 g          283 t
ORIGIN      
        1 agggttgcga agaataattg aagattactg tcatccctgc gaaggcaggg atccaaaggt
       61 tacgcatcct tctggattcc agcctgcgct ggaatgacag acacagcttg caaaaagtca
      121 taatctgcct tgagggaaaa agccccgtga ccgttcggtc tcggggcttt tgaatattgt
      181 gatctatgta ggggccgtgt tttataaagg tgtaggggcc gggctgggct ggaccggccc
      241 atgtttttgg gcgagtcacg actcgcccct acgttctatt gtgacagtga ttatcgagct
      301 ggcttaaaaa aacaatctac tatcaatggg ttacaaactc cctattgctt ttctgacaca
      361 gcgcatctat gtgccaagac atggggttag ggcagttctt gtggcagctt ttgatgggcg
      421 caatgaatgg gaattctgtc ttcaacacaa ttgggtctag aattcaaata acggcaaagc
      481 attgtgaaca ctactttatc tgatcacgct gagtgctttg ttcctgtcaa tcgatcaaag
      541 ttgtctcaat gttcttgtca ttttggaggg cgttttgtca tccctgcgaa agcagggatc
      601 caaaggttac gcatacttct ggattcctgc ctgcgcagga atgacaaaaa atatttactt
      661 tgtaacttat gaatacggcg acagaaagca agttgatttc aaacaagaat attttgaaat
      721 aaatgtgaaa tagtacgcaa catttttgcg gtgccaccga taattagtac ggagacgtag
      781 tttgtccgcg cgtgtgtcgt ggacattgat tggagggcag tagcattatg aacaacagca
      841 cccaaccaaa atatcttaaa atcatcttgg ccacagtgat cgcgttttac tcagtcgctt
      901 gcgatcagaa cattgttgct cccgtcgatt taacagtcga tacatcgatc agcatcgcca
      961 catttacagt gaccgataac atcattgagc aagagacgac tgtcacgctg aattggaacg
     1021 tcacagac
//
LOCUS       DATDUN010000202         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_465876, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000202 DATDUN010000000
VERSION     DATDUN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465876"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VJC18_02660"
     CDS             <1..192
                     /locus_tag="VJC18_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44313.1"
                     /translation="NNGNLNLIPNVPEANVDGDWSRRPNGPARESSDFKRALSEISSI
                     QTQDDDYYDIPAFIRRRAD"
     gene            213..2570
                     /locus_tag="VJC18_02665"
     CDS             213..2570
                     /locus_tag="VJC18_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HLD44314.1"
                     /translation="MKAKKRALKKPHDPIHSSLKSEIVALFLFLTGAFYYLCILGYSP
                     LDPSFFSSGSFQHITNLGGVIGAYLAGTFFFIFGGGAYFLGLYFIINSLLVFANKRSR
                     PRFLDFIVFIVCTSFIAILLQMVFIDFTFGGQSINAGGTLGKLLASLGERFLGTWGVY
                     LLVCFGAALTFVLATRLSVIDVLNTSSMALGKSLGTLSNNLYISLVRSHKGLMRYLAQ
                     RKLRSETSAQAKMKEQEFKAIKIERVKPEPPKYKPEVPQPKPELTLHQIEEMLNDQKI
                     VEPRILERKDQKAKRPPNQMELQNMSEGYQLPQLSFLDYDTSQNVEIDEDSLKMSARI
                     LESKLKDFAIEGRVTEIHPGPIITMFEFQPAPGVKLSRISGLSDDLSMAMGGRSVRIV
                     APLPNKAAIGIEIPNHVRENVWLKDIIADERFQKNDSRLLLALGKSTEGIPYVANLQK
                     MPHLLVAGSTGSGKSVSINTMITSILYKARPDEVRMILIDPKMIELQVYEGIPHLLLP
                     VVTDPKKANLALKWAVREMERRYKLLSDVNVRNLSGYNKKIDAGDFKNKTPDPESNEE
                     PIEHTGRLPYIVIVIDEFADLMMTCSKEVEESVCRLAQKARAAGLHVIVATQRPSVDV
                     ITGLIKANFPSRIAFRVTSKHDARTIMDTMGAEHLLGMGDMLFVPPGSSKMARIHGAF
                     ISEDEISRIVEFLKKQATPCYHEEILEQQEPDISAIAEEEIDELYDQAVQVVCENKRV
                     SISSIQRQFRIGYNRAARIVDQMELQGVVSAPNHQGQREVLAGTV"
     gene            2682..>3087
                     /locus_tag="VJC18_02670"
     CDS             2682..>3087
                     /locus_tag="VJC18_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HLD44315.1"
                     /translation="MKSNLWRYLIVLCLVLFSACATTAETTEAGDDAVCGDGTVAGTE
                     ECDDGNTTADDGCSATCTDESAGGGNTDPGTSLGDGLTAQSANLCDSVGGIAYSIYAQ
                     DADNIWLGCAGAIGGLFKSTDGGTNFTQVGADA"
BASE COUNT          757 a          823 c          801 g          706 t
ORIGIN      
        1 aacaacggca acctcaacct gatacccaac gtgccagaag caaatgttga cggagattgg
       61 tcgcgtcgtc ccaatggccc ggcacgtgag tcatcggatt tcaagcgagc gctgtccgag
      121 atcagcagca ttcaaactca agacgatgat tactacgata tcccggcgtt catcaggagg
      181 agggccgatt gagcatcctt gggagccaca gaatgaaagc aaaaaagcgc gctttaaaaa
      241 aaccgcatga ccccattcac agttccctca aatccgaaat tgtggccctg tttttgtttt
      301 tgacgggggc tttctactat ttgtgtattc tcggctacag ccctcttgac ccctcctttt
      361 tctcatcagg gagctttcag cacatcacca atctgggcgg tgtcattgga gcgtatctgg
      421 caggaacctt ttttttcatc ttcggtggcg gcgcgtattt tttggggttg tatttcatca
      481 tcaattccct gctggtcttt gccaacaagc ggtcacgacc tcgctttctg gattttatcg
      541 ttttcattgt gtgcacttcc ttcatagcta tcttgttgca aatggttttc atcgacttta
      601 cctttggagg acagagcatt aacgctggcg gtacgttggg caaattgctg gccagtttag
      661 gggagcgctt tttggggacc tggggcgtgt accttctggt ttgttttgga gcggccctga
      721 ccttcgtgtt ggcaactcgt ttatctgtca tcgacgtgtt gaatacctcc tcgatggctc
      781 ttggcaaaag tctggggacc ctgagcaaca acctttacat ctctctggtc cgcagccaca
      841 aaggcctcat gcgttatctg gcgcaaagaa agctgcgcag tgagacttca gcgcaagcca
      901 aaatgaagga gcaggaattc aaggccatca agatcgagcg ggtaaagcct gagcctccaa
      961 aatacaaacc tgaggttcca caaccgaaac cggaactcac cctccatcaa attgaagaaa
     1021 tgctcaacga ccagaagatt gttgaaccac gtattctgga acgcaaggac cagaaggcca
     1081 aacgcccccc caaccagatg gagttgcaaa acatgtcaga agggtatcaa ctgccacaac
     1141 tgtctttttt ggactacgac accagccaga acgtggaaat cgacgaggac agtctcaaga
     1201 tgagcgcccg cattctggag agcaagctca aggatttcgc cattgaaggg cgcgtgaccg
     1261 aaatccatcc cggtcccatc atcaccatgt ttgagtttca accggcaccg ggagtcaaac
     1321 tgtcgcgcat cagcgggcta tcggatgatc tgtccatggc catgggaggc cgttcggtcc
     1381 gcattgtggc tcccctgccc aataaggccg ccatcgggat cgaaatcccc aaccatgtgc
     1441 gggaaaacgt gtggctcaag gacatcatcg ctgacgaacg cttccagaaa aacgattctc
     1501 gtctcctgct ggccttgggt aagagcacag aaggcatccc gtacgtggcc aacctgcaga
     1561 agatgcctca cctgctggta gccggctcaa caggctcggg caaatccgtg tcgatcaaca
     1621 ccatgatcac ctcgatttta tacaaggcca gacccgacga ggtccgcatg atcttgatcg
     1681 atcccaaaat gatcgaactg caggtttacg aaggcatccc tcacctgcta ttgcctgtgg
     1741 tgaccgatcc caaaaaggcc aacctcgcac tcaagtgggc tgtgcgcgaa atggagcggc
     1801 gttacaaatt attgtccgat gtgaacgtgc gcaatttgtc cgggtacaat aaaaaaattg
     1861 atgctggcga cttcaagaac aaaacgccag atcctgagtc aaacgaggaa cccattgaac
     1921 acacgggtcg cctgccgtac attgtgattg tcatcgacga atttgctgac ctcatgatga
     1981 cctgttccaa agaggttgaa gagtccgtgt gtcgcctggc ccaaaaggcc agggccgccg
     2041 gtttgcatgt gatcgttgcc acacaaagac cttctgtgga tgtgatcacg ggcctgatca
     2101 aggccaactt tccctcgcgc atcgccttcc gcgtcacgtc caaacatgac gccagaacga
     2161 tcatggacac catgggtgcc gagcacttat tgggcatggg tgacatgctc tttgtcccgc
     2221 cgggcagttc caaaatggcg cgtattcacg gggccttcat ttccgaagac gaaatttcgc
     2281 gcatcgtcga gtttttgaaa aaacaggcca cgccctgtta ccacgaagaa atcctcgagc
     2341 aacaggaacc ggacatttct gccatagccg aagaagaaat agatgagctc tacgatcagg
     2401 ccgtgcaggt ggtgtgcgag aacaagcgtg tcagcatctc cagcattcaa cgccaattcc
     2461 gcattggcta caatcgcgcc gcccgcattg tggatcagat ggaactgcag ggtgtcgttt
     2521 cggcccccaa tcatcagggc cagagagagg tgctggcggg gacagtgtga aagacatatg
     2581 gtttgactcc acagctaagg tataatactg tgcgcgcggc tggccccgcc atctcaggca
     2641 cgggcctagt ttcaacacaa acactaataa aggagctttg aatgaaatcc aacttatggc
     2701 gatatttaat tgttttgtgc ctcgttcttt tttctgcctg tgccaccaca gcggaaacga
     2761 cagaggctgg tgatgacgcg gtttgcggcg atggcactgt cgcaggcacc gaagaatgcg
     2821 acgacggcaa cacgactgct gatgatggtt gctctgcaac gtgtacagat gaatcagcag
     2881 gcggcggcaa caccgatccc ggcaccagct tgggcgacgg gctgaccgcg caaagtgcca
     2941 atctgtgcga ctccgtggga gggattgcct attccattta cgcacaagac gccgacaaca
     3001 tttggttggg ttgcgcaggc gccattggtg gcctttttaa atcaacggat gggggcacaa
     3061 attttactca ggttggtgct gatgcca
//
LOCUS       DATDUN010000203         1162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_51028, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000203 DATDUN010000000
VERSION     DATDUN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51028"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..281
                     /locus_tag="VJC18_02675"
     CDS             105..281
                     /locus_tag="VJC18_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44316.1"
                     /translation="MLVDLMWDYFQNKTILITGASSGIGEDLALSSALPNWLFDLLIP
                     RLSGQKKDLEPRLL"
     gene            319..777
                     /locus_tag="VJC18_02680"
     CDS             319..777
                     /locus_tag="VJC18_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44317.1"
                     /translation="MYTFFGLTLETVFAVLGIDRALGFKLKRRVPQKYLEGFVSLTMI
                     PLHGLGLLFLFEPVHAFIRNWFWGFRYLTWAILISLSEVFWGWFCDKTLGFYSWDYYA
                     DSRYKVFKRGYTLWTLVPQWGLAGLVLEGYSDLMIYLSKDAVIFFEAWHY"
     gene            complement(771..962)
                     /locus_tag="VJC18_02685"
     CDS             complement(771..962)
                     /locus_tag="VJC18_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44318.1"
                     /translation="MEEKKFSPCFCTAILGLLVIVLAWWDFSWSGIALTIIGLLVIAK
                     GLINQCCCPRSKTKDSCCQ"
BASE COUNT          272 a          274 c          287 g          329 t
ORIGIN      
        1 agccggggga aaagaaaaaa ccagcgcaac aggccccatc cagcacacgc gatcacaaat
       61 tatagagtgc cccttctgcc gccgcttgca taccctgcaa gtaattgtta gtcgatctca
      121 tgtgggatta tttccaaaac aaaaccattc tcattacggg tgcctcctcc ggtatcggtg
      181 aggatctcgc cctctcgtcg gctcttccca actggttatt tgatttattg atcccccggc
      241 tttccggaca aaaaaaagac ctggaaccga ggttgttata atatgcagac ggtcgttcct
      301 ttaatttttc gctttgtcat gtacacgttt tttggactga cgctggagac ggtctttgcg
      361 gtcttgggga ttgaccgggc tttggggttc aagctgaaac gtcgtgtgcc tcaaaaatat
      421 ctcgaaggat tcgtcagtct caccatgatt cccctgcacg ggttgggtct gctgtttctc
      481 tttgaaccgg tccacgcttt catccgaaac tggttttggg ggtttcggta tttgacgtgg
      541 gcgatcctca tttctctcag cgaggttttt tggggctggt tttgcgacaa gacgctgggc
      601 ttttattcgt gggattacta tgctgactcg cgatacaagg ttttcaagcg aggatacacg
      661 ctgtggacgc tagtcccgca gtggggattg gcgggcctgg tcttggaggg ttattctgat
      721 ctgatgatct atttatcgaa ggacgcagtg atctttttcg aagcttggca ctactgacaa
      781 cacgaatcct tggtcttgga acgggggcaa caacattgat taatgaggcc cttggcaatg
      841 accagtagac caatgattgt aagcgcaatg ccggaccagc tgaaatccca ccaggcgagc
      901 acgatgacga ggagtcccaa aatggctgtg caaaaacagg gtgaaaattt tttttcttcc
      961 atgcaacaat cctttcataa aaatattcag cacacattct aacaagctgt ctgtgatgtc
     1021 aatggattat cgccgttttg ttttggcagg tattcggagg gattccaaac agcgttattg
     1081 cgtttcccga ttggcgccca aaaatttttg aatctgtttt cccacccaag ccccttgaga
     1141 cacgcaggtc tgtatcaaaa aa
//
LOCUS       DATDUN010000204         7131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_259783, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000204 DATDUN010000000
VERSION     DATDUN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259783"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VJC18_02690"
     CDS             complement(<1..478)
                     /locus_tag="VJC18_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HLD44319.1"
                     /translation="MTRKIKSGIIQCSLPMHEGEGSIEAITSAMFEKHIPFIEKAGKQ
                     GVQILCMQEIFTTPYFCPSQDIKWYKTAEKVPGPTVDRLKEYAKKYQMVIVAPIYEEE
                     MTGVYYNTAAVIDADGKYLGKYRKNHIPQVAGFWEKFYFKPGNLGYPVFDTRYGKVG"
     gene            complement(587..988)
                     /locus_tag="VJC18_02695"
     CDS             complement(587..988)
                     /locus_tag="VJC18_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44320.1"
                     /translation="MELTNFGSIMKFAIDRETDLKDILVRAIADPKLSSLADQLTAMT
                     AQNQSNLKLLERTRREGICEMVLHPITGLNSDDYVFAADPHALGSADGFVSFIKIASE
                     KLMRFYATAGEKLPADDAKRAFLKLAKKPYL"
     gene            complement(1184..2635)
                     /locus_tag="VJC18_02700"
     CDS             complement(1184..2635)
                     /locus_tag="VJC18_02700"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HLD44321.1"
                     /translation="MKMLKNYIDGEWVDSSSKNFLEVRNPASAEVLAKVPLSTATEVD
                     VAVQAAQKAYWEWRTTPPVQRARYFYRLKDVLENNFEELAKLTTIEAGKTMDESRGEL
                     RRAIEMVEVACGIPSLMMGACLEDITKNIDSTAFKQPMGVFAAVAPYNFPLMVPFWFW
                     PFAVACGNTFVLKPSEQVPQSQIRIMEIIDEEIGFPPGVLNLVNGGKETVTALLEHPL
                     IKGVSFVGSSPVARIIYSKCGETGKRVQSLGGAKNFHIIMPDANIKETVGAIMGSAFG
                     CAGQRCLATSVLLAVGDVYEPLKKAVVEAASKIKVGYGLDESVQMGPVISKAHKEKVL
                     SYINKGLEEGAELLLDGRKIKVSGFEEGYFIGPTVFDKLKPEMVIANEEIFGPVLGIM
                     QVKTMDEGIEIIKKNPFGNSTSIFTASGKAAREFVYKAECSMMGINIGIAAPMAFFPF
                     GGTKGSFFGDIKGHGREIVDFFTDRKVVVTRWV"
     gene            complement(2742..2999)
                     /locus_tag="VJC18_02705"
     CDS             complement(2742..2999)
                     /locus_tag="VJC18_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLD44322.1"
                     /translation="MHYVYLLQCRDGSLYTGVATDLEKRLAQHMAGKGSKYVRSRLPF
                     TLVYREECESQSEALKRECHIKSLSRAEKLVLIKDISKNPA"
     gene            complement(2999..3877)
                     /locus_tag="VJC18_02710"
     CDS             complement(2999..3877)
                     /locus_tag="VJC18_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD44323.1"
                     /translation="MFTPLRSSSFVGQACSRGGIMDVLQILDYSDYRVFLKELYERRK
                     QISGKYSYRQYADDLGFSASNYLHLVVAGQRNLSPSAAAKIMVHFKWTAQQKKYFRAL
                     VLYNQSTKADEQEACFRALEKILGKKRVMIGPDQAAYFSRWYLPVLKEMVALKNFVPH
                     LNWISKTLFPCVDEPLVKEGLAILERLKMIKRVKDRWVQEAEHLTTPLQINSSMVHGY
                     HREMLKLSANALNMDSAHRDISTMTMSLSQRQFDWLKQRVIDFRDEVQQELQGMRDEQ
                     TLVAQLNIQLFPVTRT"
     gene            4053..4874
                     /locus_tag="VJC18_02715"
     CDS             4053..4874
                     /locus_tag="VJC18_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44324.1"
                     /translation="MVSFVICSFLFCLCRCDCGGSGTTQVGNSSPTDPIVTVTISKIS
                     EDDSLTIYIPLSVIGTNTSSLVETTNDIVINGEEYASDLTALTYYDSANEQVTLEVLN
                     MVDGDQIVITINKPDDTFETYAATASTASDATAILTDSGVIQNEAVPEPEPDPDTYTA
                     ASTLVATLCAEITACVPELSEFVCLIGVGIAPNLSGYFGVDGADAALSLAELETAINE
                     GTYTANEDNLAQCVVDLEAITCAEIIGAYSLDDPVNFEGLTNLIPTDPASCPDVF"
     gene            5076..5969
                     /locus_tag="VJC18_02720"
     CDS             5076..5969
                     /locus_tag="VJC18_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44325.1"
                     /translation="MTMTFPSLKKYLMATGLTIFALMSMSCAAIHSNELASMLAEDLV
                     LEASIYEYSSDDEAQVSAQMETESSGIGIEFNQGESLRAYADANADTEAGISNDVYLS
                     NNAILNLDQDYSGEIDKTTDGGNYYITYTDPDGNETTAIIPSFSVPEITNIENGDILS
                     GTEAVIEWEPTDVSGLLSIVVSYSGSGMVGYRRKYVSNTGSYTMDIEGCVGSGTVDLR
                     STMNLNYMEGFGGANIEMNNNSRRYVSYSNTLASALVKGLSSTEAPPVEQLIAQCQTK
                     CGERDEAYILVDETKYDCCVR"
     gene            complement(6041..6114)
                     /locus_tag="VJC18_02725"
     tRNA            complement(6041..6114)
                     /locus_tag="VJC18_02725"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6078..6080),aa:Pro,seq:cgg)
     gene            complement(6289..>7131)
                     /locus_tag="VJC18_02730"
     CDS             complement(6289..>7131)
                     /locus_tag="VJC18_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLD44326.1"
                     /translation="YSEQGGASWITFSSLGGTAVLDQLTVAGNTNGGGEVPVYNSMTF
                     QSLDRNVDTEVQLSHSLFENNYGSGLYLTDISNSQIDDLVMRDNVGKAIVLGDVGSAV
                     LTNLDISGQELGAIWAYNMWRTEGTYLEIGNSRIHDNVLTFDEMYGNENVTAGLTLNS
                     YTEPMQVVIADSEVVDNDSTQNTGYGRNKAGAIVLIEADLTVRNSSVLRNVTSGSDYL
                     AGGITYSEGAHVTVENSNFGTDENENIPYDTAYFNAPGHWYYNFGDGVNFECDGATHI
                     CTEW"
BASE COUNT         1810 a         1918 c         1600 g         1803 t
ORIGIN      
        1 cacccacctt gccatagcgg gtatcgaaaa ccggataacc cagattgccg ggcttgaaat
       61 aaaatttttc ccaaaaccct gccacctgcg gaatatgatt tttgcggtac ttgcccaaat
      121 acttgccatc agcatcgatc acggccgctg tgttgtaata cacgcctgtc atttcttctt
      181 cgtagatggg agccacaatg accatctggt attttttggc gtactctttc aatcgatcaa
      241 ccgttggtcc gggaaccttc tcggcagttt tgtaccattt gatgtcttgt gagggacaaa
      301 aatatggtgt ggtgaagatc tcctgcatgc aaaggatttg cacgccctgt ttgccggcct
      361 tttcgatgaa gggaatgtgt ttttcgaaca tcgccgacgt gatggcctcg atgctaccct
      421 caccttcatg catgggaagt gaacattgta tgattcctga tttaatttta cgggtcatga
      481 tggcctcctg ttaggcgtgg atcgttgttc gtgaatcgtg gatcgcctgt gccccaaagg
      541 gtacttgtgc ccgttagggt atgattcacg atcaacgatg cacgattcac aagtatggtt
      601 ttttcgccaa cttcaaaaac gccctcttgg catcgtccgc aggcagtttt tctccagctg
      661 tggcataaaa ccgcataagc ttttcactcg ctattttgat gaaggagaca aagccatcgg
      721 cactacccaa ggcgtgtgga tcagcggcga acacataatc atctgagttt aaacccgtga
      781 tggggtgcaa taccatctca caaatcccct cgcgtctggt ccgttccagc aacttaagat
      841 tggactgatt ctgcgccgtc attgctgtga gctgatcagc aagcgatgac agtttgggat
      901 cggcaatagc ccttaccaaa atgtccttga ggtcggtttc gcgatcaatc gcgaacttca
      961 tgattgaacc aaagtttgtc aattccataa tacattccca tcctcaagaa tggctgtcat
     1021 gtttgttttc gcatgtgaga cgccaaggcc ggcacaaaac aacaaataat gacaaacgcc
     1081 gtgttttcat ggccgagtaa ccgcaggccg tttgccccgg caatgatacc acttcggccg
     1141 actgtctcac atgcgaaaac aaacatgaca gccattcttc tttttatacc caccgtgtga
     1201 ccaccacttt tcgatcggtg aaaaaatcca cgatctcacg accatgcccc ttgatgtcgc
     1261 caaagaatga acccttggta ccaccaaacg ggaaaaacgc catgggcgcc gcaatgccga
     1321 tgttgatgcc catcatactg cactctgctt tgtacacaaa ctcgcgcgcc gctttgccac
     1381 tcgccgtaaa gatcgacgtg gaattgccaa atgggttctt tttgatgatc tcaatcccct
     1441 cgtccatggt cttcacctgc atgatgccaa gcacgggtcc aaagatttct tcgttggcaa
     1501 tgaccatctc gggcttgagt ttgtcaaaaa ctgttgggcc aatgaaatag ccttcttcaa
     1561 agccactcac cttgatctta cgaccatcca gcagaagttc ggcgccctct tcgagcccct
     1621 tgttaatgta ggaaagcact ttttccttgt gggctttgga aatgacaggg cccatctgca
     1681 cggactcatc caaaccgtaa cccaccttga tcttggaggc ggcctcgacc acagcctttt
     1741 tgagaggctc ataaacatca ccaacagcga gcaaaacact ggtggccaaa caccgctgac
     1801 ccgcacaacc aaatgccgag cccatgatgg cccccacggt ttctttgatg ttggcatcgg
     1861 gcatgatgat gtgaaagttt ttggcaccgc ccaacgactg aacccttttg cccgtttcac
     1921 cgcacttgct gtatatgatg cgggccactg ggctagagcc cacaaaggac acgcctttga
     1981 tgagaggatg ttctagcagt gcagtgactg tctctttgcc accattaacg agattaagca
     2041 cgccaggggg gaaaccgatt tcttcatcga tgatttccat gatccgtatc tgactttgcg
     2101 gaacctgttc ggacggtttg agcacaaagg tattgccaca agccacggca aagggccaaa
     2161 accagaaagg caccatcagt ggaaaattat aaggggccac cgcggcaaac acacccatcg
     2221 gttgtttgaa cgctgtagaa tcaatatttt tggtgatgtc ctcgagacag gcccccatca
     2281 tcaagctggg tatgccacag gccacttcga ccatttcgat cgcccgacgc aactcaccac
     2341 gggattcatc catcgtctta ccagcctcga tggttgtgag tttggccagt tcctcaaaat
     2401 tattctcgag cacatcttta agacgataaa aatagcgtgc acgttgcacc ggaggggtgg
     2461 tccgccattc ccaataggcc ttttgggccg cctgcactgc cacatcaact tctgttgccg
     2521 tagaaagtgg caccttggca agcacctctg ccgaagcggg gtttctgacc tcaagaaaat
     2581 ttttggaact ggaatcgacc cattcaccat caatataatt ttttaacatc ttcataacct
     2641 atttgcctcc taaaataaat gtgtgtgcga gaccctaggc aggaatcaaa ataacgtcaa
     2701 agatttttta actccccctg ccccctctca taacaggggg ttcatgcggg gtttttagag
     2761 atgtccttaa tcaaaacaag tttttcagcg cgtgacaggc ttttgatgtg gcactctcgc
     2821 ttgagcgcct ctgattgaga ttcgcactct tcacgataca ccaaggtaaa gggcaatcgg
     2881 gaccggacat atttggaccc cttgccagcc atgtgctgag ccaacctttt ttcaaggtcg
     2941 gtggcaacac cagtgtacag gctcccatcg cggcattgta gaagataaac gtaatgcatc
     3001 atgttctcgt cacaggaaac agctggatgt tgagttgtgc caccagggtc tgctcatcgc
     3061 gcatgccctg cagttcctgt tgcacctcat ccctaaagtc aatcacacgc tgtttaagcc
     3121 agtcaaactg cctctgagaa agactcatcg tcatcgtgga aatatcgcga tgggccgaat
     3181 ccatattcag cgcatttgcc gataatttga gcatctctct atgataaccg tgcaccattg
     3241 atgaattaat ctgcagtggg gtcgtgaggt gctcggcctc ctgcacccac cgatccttaa
     3301 cccgcttgat cattttcagc cgctccaaaa tggcaagccc ctccttcacc aatggctcat
     3361 cgacacacgg aaaaagagtt ttggaaatcc aattgaggtg ggggacaaaa tttttcaaag
     3421 caaccatctc tttcaagacg ggcagatacc acctgctgaa ataggccgcc tgatcgggtc
     3481 ctatcatgac ccttttcttg cccagaattt tttcgagtgc ccgaaaacag gcttcctgtt
     3541 cgtcagcctt tgtcgactgg ttataaagca ccagtgccct gaaatatttt ttttgctgag
     3601 ccgtccattt gaaatggacc ataatttttg ccgccgccga gggtgagaga ttcctctgcc
     3661 ccgcaacaac cagatggagg taatttgacg ccgaaaaccc caggtcatcg gcatactgcc
     3721 tgtaggaata tttcccactg atctgttttc gtcgttcgta cagctctttg agaaacaccc
     3781 tgtaatcact gtaatccaat atctgcaaca cgtccataat tccccctcgt gaacacgcct
     3841 gccccacaaa gctcgaagag cgtagtgggg tgaacacgtg aacacgtaaa cacgtgaaca
     3901 cgtgaacacg cccttgcatc gtatcatgca gggcatgcct gcccggatct gtatacaatg
     3961 tatacaataa atgcaaatct ccacattttt ttgctgtcat tttttgggga gcttgttaga
     4021 ccagtgacat gagttcccct caccgatttt ttatggtgtc ttttgtcatc tgttcttttt
     4081 tgttctgcct gtgccgttgt gattgcggcg gaagcggcac cacacaggtg ggaaactcat
     4141 cacccacaga tcccattgtg acagtgacca tcagcaaaat ttctgaggat gattcactta
     4201 ccatttacat ccccttgagc gtcattggca cgaatacaag ctcgctggtt gaaaccacca
     4261 acgacatcgt catcaatggc gaggaatatg caagcgatct caccgcgctg acctattacg
     4321 acagcgccaa tgagcaggtc accttggaag tcctgaacat ggtggatggc gatcagatcg
     4381 tgatcaccat caacaaaccc gatgatacct ttgagaccta cgcggccaca gcctcgactg
     4441 cttcggatgc tacggcgatc ctgaccgatt caggagtcat ccagaatgag gcagttcccg
     4501 aacctgaacc cgatcccgat acctacacgg cggcatcaac gctcgtagcc actctctgcg
     4561 ctgaaatcac agcgtgtgtc cccgaacttt cggaattcgt ctgcctgatt ggtgtgggaa
     4621 tcgcccccaa cctgtccggt tattttggtg tcgacggcgc cgatgccgcc ctgtcactcg
     4681 ccgaactgga aacagctatc aacgaaggaa cctacactgc caatgaggac aacctcgcac
     4741 aatgcgttgt tgatcttgaa gccatcacct gcgccgagat tatcggcgct tattcccttg
     4801 atgatcccgt caattttgag ggtcttacca acctgatccc caccgatcct gccagttgtc
     4861 cggatgtatt ttagcttttc tgtttacgcc cataacccat tacccacctg ccctgagcga
     4921 ttgatcccga gtactgtcga gggaagtcga agggttaccc atcctgtata cactgtatac
     4981 aagataaaga caaaaaccct cttttggggg catctttcgt gtcccaaacg cgataatacg
     5041 aaagaaggaa caataaccct aatacggagg tttatatgac catgactttc ccatctctta
     5101 aaaaatatct catggcgaca ggccttacca tattcgcact catgagcatg agttgcgccg
     5161 ccattcatag taacgagctg gcctcaatgc tggctgagga cctcgtcctc gaggcatcaa
     5221 tctatgagta ctccagtgat gacgaggcgc aggtcagcgc gcagatggaa acggaatcga
     5281 gcggaatcgg gattgaattc aatcagggcg agagcctcag ggcctacgcc gatgccaatg
     5341 cagatacaga agcagggatc tcgaacgatg tttatctatc gaacaacgcc atcttgaatc
     5401 tggatcagga ctacagcggg gagatcgata aaaccactga cggcggaaac tattacatca
     5461 cctacacaga tcccgatggc aacgagacca cggccatcat cccctctttt tccgtccccg
     5521 agatcaccaa cattgaaaac ggtgacatcc tgtcgggcac tgaagccgtg atcgagtggg
     5581 aacccacgga tgtctctgga ttgctttcca ttgtagtcag ttattcggga agcggcatgg
     5641 tagggtatcg cagaaaatat gtgagcaaca caggttccta caccatggac attgaaggct
     5701 gtgtgggatc gggcacagtc gacttaagaa gcaccatgaa cttaaattac atggagggtt
     5761 ttggtggcgc taatattgaa atgaacaata actcccgccg ctatgtaagt tattccaaca
     5821 cgctggcctc tgcccttgtt aaaggcctct cctcaacgga agccccgcct gtagaacaac
     5881 tgatcgcaca atgccagacc aaatgcggtg agcgagacga ggcttacatt ttggttgatg
     5941 aaaccaaata tgattgttgt gtgcggtgaa ggaattggcc tgcatcccct gaatcgcagc
     6001 aaccaaaaaa ggcaaccctc tggttgcctt ttttgttatg tcggggatac gagatttgaa
     6061 ctcgtggcct ttcggtcccg aaccgaacgc gctaccgggc tgcgctaatc cccgctctat
     6121 tcaatctgtc attgcgagcg aaactttagt tgagcgtggc aatctccgct aacaatctgt
     6181 tcaaggggat agccacgtct cgtttcactc acctcgctat gacacatacc ccgacggcct
     6241 atacggtatt ttgaatcaaa aagacaaggg gtttgaagaa gaagaaaatc accattctgt
     6301 acagatatgt gtggcaccat cacactcaaa attcacaccg tccccgaagt tgtaatacca
     6361 atgcccaggg gcattgaaat acgctgtgtc gtatggtatg ttttcatttt catcggtacc
     6421 aaaattcgaa ttttcaaccg tcacatgggc gccttcagaa taggtgatcc caccggcaag
     6481 atagtcagaa ccagatgtca catttcttaa tacggatgag ttccttactg tcaaatcagc
     6541 ttctatgaga acgatggcgc cagccttatt cctgccataa ccagtatttt gtgtggaatc
     6601 attatcaacc acctctgaat cggcaatcac gacctgcatg ggttctgtat aactatttag
     6661 cgtcaggcct gccgtcacgt tctcatttcc atacatttcg tcaaaggtaa gcacattatc
     6721 atgaattcgg gagttgccaa tttccaaata cgtgccctcg gtacgccaca tattgtatgc
     6781 ccagatggcc cccaattctt gcccactaat atcgagattt gtgagcacgg cagagccaac
     6841 atcgcccagc actatcgctt ttcccacatt gtcgcgcatg accaaatcat cgatttgact
     6901 gtttgagatg tcagtcagat aaagaccact gccatagttg ttttcaaaca aagaatgact
     6961 gagttgcacc tcagtatcca cgttgcgatc gaggctctga aacgtcattg agttgtaaac
     7021 aggcacctcc cccccaccgt ttgtgtttcc tgccaccgtc aactgatcca acactgccgt
     7081 cccacccaaa gaagaaaatg tgatccacga ggcaccgccc tgttccgaat a
//
LOCUS       DATDUN010000205         1945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_384265, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000205 DATDUN010000000
VERSION     DATDUN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384265"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VJC18_02735"
     CDS             <1..674
                     /locus_tag="VJC18_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HLD44327.1"
                     /translation="CQFSSFQVDLTAQESDLFAGLHHKHRNVIRKAMAEGVQIVSGTE
                     YLDECARLIQQTMQRQSLPWARKGELMRLAAELGDQVSFYVAKKNGKSQGCAVVIWKA
                     GSSAYYLHGGSIEGPSLGAMNYLQWKIMLDMKAHGVLVYDFMGARMSPLPGSRQESIQ
                     RFKSRFGGQLKVGYLWKYPLKPLRYKLFTATAKLNAYARGLKYTGDIIDQERARSHES
                     DCSHI"
     gene            649..1644
                     /locus_tag="VJC18_02740"
     CDS             649..1644
                     /locus_tag="VJC18_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLD44328.1"
                     /translation="MNQIVLTFDYELFLGAHSGTAQKCLLEPTSAILQILKEHNATGI
                     FFIDASYLLHLVAQRHPDFEKVRAQIIEMAKAGHDFGLHLHPQWLDAVPLNGNEWTFK
                     THERYRLQSLGERERGALIASGKEMIEEIVQQVCPAYKVQAFRAGGWCVQPFQELKNH
                     MLSQGLHYDFSVRPGIANNDNEQAAYDFTKAPQEKDYWRFENDPCLPQELGKFFEVPM
                     TSVKISGRTLLKNYFQMRRSARCGDGQGLRKPKPLAKQILRLLLSELRSSTLDLASVD
                     LVMKQLKKGRQKKRLFQVWVHHPKLWTAKTHQALKTLLLETKTLSFSDVKRIVSA"
     gene            1641..>1945
                     /locus_tag="VJC18_02745"
     CDS             1641..>1945
                     /locus_tag="VJC18_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44329.1"
                     /translation="MTKMRVAILPRVIQDYRVSVYERLSQVESLHIKVFHGCDVKGTK
                     LVNSKKSHSFASKEMFSIPIRISTSNGKGYSSFSPFVFIELLRFSPDVVVVKGASNFL
                     "
BASE COUNT          562 a          361 c          486 g          536 t
ORIGIN      
        1 attgccagtt cagttcattc caagttgatt tgacagcaca ggaaagtgat ttgtttgccg
       61 ggctccatca caaacataga aatgtcatca gaaaggcaat ggcggaggga gtgcaaattg
      121 tatcagggac tgaatatctg gatgaatgtg cacgtttgat acaacagacc atgcaacgac
      181 aatccctccc gtgggcaagg aaaggtgaat taatgaggtt ggccgccgag ttgggagacc
      241 aagtttcatt ttatgtggca aaaaaaaatg gtaaaagtca gggttgtgcc gttgtgattt
      301 ggaaagcagg gagctctgca tattatttgc atggtggcag tattgaagga ccaagtttgg
      361 gagcaatgaa ttatttgcag tggaagatca tgttggacat gaaggcgcac ggagtgcttg
      421 tgtatgattt tatgggtgcc agaatgtcgc cgctaccagg ctccaggcag gaaagtattc
      481 agcggttcaa atcgcgtttt ggaggccagc tgaaggtggg ctatttgtgg aaataccctt
      541 tgaaaccctt gcgctataag ttgttcacag ccacggccaa actgaatgcc tatgccaggg
      601 ggttgaaata tacaggcgac attatcgatc aagaaagagc acgatcacat gaatcagatt
      661 gttctcacat ttgattatga gctttttttg ggtgctcata gcgggacagc acagaaatgt
      721 ctccttgaac cgacatctgc tattttacag atattgaaag aacacaatgc gacaggcatc
      781 ttctttattg atgccagcta ccttttgcat cttgttgcac aacgacaccc tgattttgaa
      841 aaagtcaggg cacaaatcat tgagatggca aaagcgggcc atgattttgg tttgcatttg
      901 catccacaat ggcttgatgc tgtaccgcta aatggtaacg agtggacatt caagactcac
      961 gagagatacc ggttacaatc cttgggtgag cgcgagagag gtgctttgat tgcaagtggc
     1021 aaggagatga ttgaagagat tgtccagcaa gtttgtccag catacaaagt gcaggccttc
     1081 agggctggtg gctggtgtgt tcagccgttt caagaactga agaatcacat gttatcacag
     1141 ggtttgcact atgattttag tgtgcggcca gggattgcaa acaatgataa cgagcaagca
     1201 gcatatgatt ttacaaaagc accgcaggaa aaagattact ggcgttttga aaatgatccg
     1261 tgtttgccac aagaactggg taagtttttt gaagtaccca tgacttcggt gaagatttca
     1321 ggaaggacct tgttaaaaaa ttatttccaa atgcgcagga gcgctcgttg tggtgatggt
     1381 cagggattgc gtaagcccaa gccgttggca aaacaaattt tacgattact attgtctgaa
     1441 ttgaggtcat ctacccttga tttggctagt gttgatttgg tcatgaaaca actcaaaaaa
     1501 ggcagacaaa aaaaacggtt atttcaagtg tgggtgcatc atcccaagtt gtggacagca
     1561 aagactcatc aggcgttaaa aaccctgctt ttggaaacaa agacattatc tttttctgac
     1621 gttaaaagga tagtgtctgc atgacaaaaa tgcgtgttgc catattgcct cgtgtcattc
     1681 aagattaccg ggtctcagtt tacgaacgct tgagtcaggt tgaaagtttg cacatcaaag
     1741 tatttcatgg ctgtgatgtg aaaggcacta agcttgttaa ttcaaaaaaa tcgcatagct
     1801 ttgcatcaaa agaaatgttt tcaataccca ttcggatcag cacatcaaat ggaaaaggtt
     1861 actcgtcatt cagtccgttt gtgtttattg aacttctcag gttctcaccc gatgtggttg
     1921 ttgtaaaggg ggcctccaat tttct
//
LOCUS       DATDUN010000206         1462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_425318, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000206 DATDUN010000000
VERSION     DATDUN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425318"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VJC18_02750"
     CDS             <1..1368
                     /locus_tag="VJC18_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44330.1"
                     /translation="AGGTMDASNLLKPALTNGELKCIGSTTFEDFRRVFEKDHALARR
                     FQKIDINEPTHDETVRILKGLKSRFEEHHNVKYTDAALKAAVELAGRYIHDKKLPDKA
                     IDVIDEAGSANRLQNQTKRKKTIDVVAIENIVAKIARVPVRTVSKDDKINLKSLDQEI
                     KSVVFGQDHAIEQVVTSIKLSRSGLGDAGKPIGSFIFAGPTGVGKTEMAKELARIMGI
                     EFVRFDMSEYQEKHTVSRLIGAPPGYVGFDQGGLLTDQLHKTPHAILLLDEIEKAHPD
                     LFNILLQIMDYGTLTDNNGRKTDFKNVVIIMTTNAGARDMNRQTIGIIKSTSTAQDGK
                     KELERIFSPEFRNRLNGIIYFNPLPSSVVLLIVDKFLREISKQLQEKKVRLQITDDTR
                     EWLARKGYDEKMGARPLARLMHNEIKKPLSEEILFGKLEQGGQVVIEVKDDALSFDYS
                     VPVPA"
BASE COUNT          386 a          370 c          389 g          317 t
ORIGIN      
        1 gcgggtggca cgatggacgc ctccaacctg ctcaaaccgg cgctgaccaa cggagagctc
       61 aaatgcattg gctccactac cttcgaagac tttcgtcgcg ttttcgaaaa agatcatgcc
      121 ctggcccggc ggtttcaaaa aatcgatatc aacgaaccca cccacgatga aacggtcagg
      181 atattaaaag gactcaaatc ccgttttgag gagcatcata acgtcaaata cacggacgcg
      241 gcgctcaagg cggctgttga gctggcggga cgatacattc acgataaaaa attgcccgac
      301 aaggcgatcg atgtgatcga tgaggccggc tccgcgaatc gtttgcagaa tcaaaccaag
      361 cgaaaaaaga ccattgatgt tgtggccatt gagaacatcg tggccaaaat cgcgcgcgtg
      421 cccgtgcgca cggtgtcgaa ggacgacaag atcaatctca aatcgcttga tcaggagatc
      481 aagtctgtcg tgtttgggca ggaccacgcg attgagcagg tggtgacctc gatcaaactc
      541 tcccgttccg gtctgggcga tgccggcaaa cccattggta gttttatctt tgcgggaccc
      601 acaggtgtgg gcaagaccga aatggccaag gagttggccc ggatcatggg gattgagttt
      661 gtgcgtttcg atatgagcga gtaccaggaa aagcacacgg tgtcgcggct catcggcgcc
      721 cctcccgggt atgtgggatt tgatcaggga ggactgctca cggatcaact gcacaaaacg
      781 ccgcatgcca ttctgctttt ggatgaaatc gaaaaggccc accccgacct gttcaacatc
      841 cttttgcaga tcatggatta tggtaccttg actgacaata acggacgcaa gacagatttt
      901 aaaaatgtgg ttatcatcat gaccaccaac gccggtgcgc gcgacatgaa ccgtcaaacg
      961 atcgggatca tcaagtccac cagtaccgcg caagacggca aaaaagagct ggaacgcatc
     1021 tttagtcccg agtttcgcaa tcgtctgaat gggatcatct atttcaatcc cttaccgtca
     1081 tctgtggtgc ttttgattgt cgacaaattt ctcagggaaa tttccaaaca gcttcaagag
     1141 aaaaaagtgc ggcttcagat cactgatgac acgcgtgaat ggctggcccg aaagggttac
     1201 gacgaaaaga tgggggcccg cccgctggcg cggctcatgc acaatgagat caaaaaaccg
     1261 ctgtccgagg aaatcctttt tggcaaactt gagcagggcg gtcaggtcgt gatcgaggtc
     1321 aaggacgacg cgttgtcttt tgattattcc gtccctgtcc ccgcgtaagg tgccagtcta
     1381 caatcggaca aaaaagtgcc acccacattt ccggcgttat ggtggggatg ctcggctgaa
     1441 tatagctcaa ttgatatcaa tc
//
LOCUS       DATDUN010000207         1845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_175301, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000207 DATDUN010000000
VERSION     DATDUN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175301"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VJC18_02755"
     CDS             complement(<1..497)
                     /locus_tag="VJC18_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLD44331.1"
                     /translation="MNKIIIKNGRVIDPASNTDGAFDVVIENTKIGDIVPRGSLSVAN
                     AQTIDATNMIVAPGFVDLHCHLREPGFEYKETIESGTAAAVAGGFTSVCCMANTSPVN
                     DNATVAEFILKKARETGHCHVYPIGAITKGLNGEELASIAELRDCGCVAISDDGNTIQ
                     NAQLM"
     gene            complement(502..1437)
                     /locus_tag="VJC18_02760"
     CDS             complement(502..1437)
                     /locus_tag="VJC18_02760"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HLD44332.1"
                     /translation="MKLNRKDILGIADLSRDEIEGILETARRFAEVNKRAIKKVPTLR
                     GKTVINVFFENSTRTRTSFEIAGKRLSADVINISSSTSATSKGESLLDTAKNLEAMQP
                     DILVCRHKSAGASSILARYLKKTSVINAGDGAHEHPSQALLDMLTVLNVKKKVDGLRV
                     AIVGDISHSRVARSNIYCFQKMGASVAVVGPASMMPCAIKDLGVDVFHQLKKGIEGAD
                     IIMMLRIQMERIKQSPFPSLREYSHYYGLNADTIKAMKKDALIMHPGPVNRGVEIDPD
                     VADGPYSVILDQVTNGVSVRMALLYLLSGAQNMEL"
     gene            complement(1515..>1845)
                     /locus_tag="VJC18_02765"
     CDS             complement(1515..>1845)
                     /locus_tag="VJC18_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HLD44333.1"
                     /translation="RDDLSECADTPILKETRIDFDVNGKTIFLADDVLYTGRTIRAAL
                     DALFDLGRPRSVHLVVMAKRSGRELPIDTDLAVLNIKTQPTDNIKVKFVETDSVDEIV
                     LLEKGEY"
BASE COUNT          408 a          455 c          505 g          477 t
ORIGIN      
        1 ttcatgagtt gggcgttttg gatggtgttg ccgtcatcag aaatggcgac acacccgcaa
       61 tcgcgcagtt ccgcaatgct ggccaactct tcaccgttga gccccttggt gatggcgcct
      121 atcgggtaaa cgtgacaatg accggtttca cgggcttttt tgagaatgaa ctcagcgaca
      181 gtggcgttat cgttgacagg actggtgttg gccatgcaac agaccgaagt gaaccctccg
      241 gccactgcgg cggcggtgcc agattcgatg gtttccttgt attcaaaacc gggctccctg
      301 aggtggcaat gcaggtccac aaacccgggc gccacgatca tgtttgtggc atcgatcgtt
      361 tgcgcgttgg ccacagacaa ggagccgcgg gggacaatgt caccaatctt ggtgttttca
      421 atcacaacat caaaggcccc atcggtgttt gaggcggggt cgatgacacg accattttta
      481 ataatgattt tattcatagc atcacaactc catgttttgt gctccgctga gcaaatacaa
      541 caaggccatt ctgacggaga cgccattggt gacctggtcg aggatgaccg aataggggcc
      601 atcggccaca tcgggatcga tttcgactcc gcgattgacg ggacccggat gcatgatgag
      661 ggcatctttt ttcatggcct tgatcgtgtc ggcattgagg ccataataat gagagtactc
      721 cctgagtgag gggaagggcg attgtttgat gcgttccatc tggatgcgga gcatcatgat
      781 gatgtcggcg ccctcgattc cctttttgag ctggtgaaaa acatccacac ccaaatcctt
      841 gatggcacaa ggcatcatgg aggcggggcc gaccacggcc acgctggcgc ccattttttg
      901 aaaacagtat atgttcgaac gggccactcg tgagtgcgaa atatcgccaa caatcgcgac
      961 cctgaggcca tccacttttt tcttcacatt caaaacggtc agcatatcca acagggcttg
     1021 agacgggtgt tcgtgggcgc catcacccgc gttgatgacg gaggtttttt tgaggtagcg
     1081 cgccagaatg ctggaggcac ccgcgctttt gtgacgacag accagaatgt ccggttgcat
     1141 ggcctcaaga ttcttggctg tgtcgaggag cgattctccc tttgacgtgg cgctggttga
     1201 tgaggagatg ttgatcacat cggcagaaag tcttttgccc gcaatctcaa aactggtgcg
     1261 tgtgcgcgtg gagttttcaa aaaaaacatt gatgacggtc ttgcctctga gtgtgggcac
     1321 ctttttgatg gcgcgtttgt tgacctcggc aaaacgacgg gctgtttcga ggatgccctc
     1381 gatttcgtca cgcgacaaat cagcgatgcc cagtatgtct ttacggttga gtttcatgcg
     1441 cgtatcgtgg atcgcgtatc gtggatcgtg gataactctt ttcgatccac gatacgcgat
     1501 ccacgaccca cgagttaata ttcgcctttt tctagcaata caatttcgtc tacagaatct
     1561 gtttcgacaa atttgacttt gatgttgtct gttggttgtg ttttgatgtt cagcactgcc
     1621 aagtcagtat cgataggcag ttctcgcccc gatctttttg ccatgaccac caaatgtacg
     1681 gaacgtggcc ttcctagatc gaagagagcg tccagcgcgg cgcgaatggt gcgaccagtg
     1741 tacaacacat catctgccag aaaaatggtt ttgccattca catcgaaatc gattctggtt
     1801 tccttgagga tgggcgtgtc ggcgcattca gacaagtcat cgcgg
//
LOCUS       DATDUN010000208         1056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_175396, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000208 DATDUN010000000
VERSION     DATDUN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175396"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VJC18_02770"
     CDS             <1..420
                     /locus_tag="VJC18_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44334.1"
                     /translation="CLYCQTERLVGGVSLSFRFSAYENWNSDEFAMAWHDVMAHLCKV
                     GENGEWEIIDDTPLINPLTGRTIPNSHITIETLISYDSQATTAIRSGAKFMDISSQLA
                     LRFCLMNGDLSFFHLYTHGFGIMPRTPNQIRQWARTL"
     gene            692..>1056
                     /locus_tag="VJC18_02775"
     CDS             692..>1056
                     /locus_tag="VJC18_02775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HLD44335.1"
                     /translation="MAVRIQQALNHHDKERVYRFRYTVLDPDFVPEQTEIDHERGLIF
                     DSHDEHAAIFYAQKGPDIVGSIRVITDLPQQLSEQGMNLYQCHELITRLQPHQTSVTD
                     MFVLDPKVRGQTLVSLLWSA"
BASE COUNT          285 a          263 c          230 g          278 t
ORIGIN      
        1 tgcctttact gccagacaga aagattggtg ggcggcgtct ctctcagttt cagattttcc
       61 gcatatgaga actggaatag tgacgaattc gctatggcct ggcatgatgt tatggcacac
      121 ttatgcaagg taggagaaaa cggcgaatgg gaaatcattg atgacacccc gctcatcaat
      181 ccccttacag gcaggaccat cccaaacagt cacatcacca tcgaaaccct gatctcatat
      241 gactcgcaag cgactactgc catacgatcc ggggcaaaat ttatggatat ttcttctcaa
      301 ctcgctctgc gattttgctt gatgaacggc gacctcagct ttttccacct ctacacccac
      361 ggttttggta tcatgccacg gactcccaat cagataagac aatgggcaag aactctttag
      421 ggccggtcac gaccggccct tgaaaaggtc acgaccggcc cttgaaaagg tcacgaccgg
      481 cctctacact ctttacccgg aaacaattgc ctttcaatag gcaatatctg ccagtttttt
      541 gacgtgacga tctgttacga aaagattttc catcaattac cgtaaattgc cacatgctga
      601 cggcatatct aatctggtcc aatttttgca gtgtgtataa gtgtcaaatc atttgaactt
      661 attgggtaat ataaaccact aggaacccat catggctgtc aggatacagc aggccctcaa
      721 tcatcatgat aaagagcgag tgtatcggtt caggtacaca gtgttggatc ctgattttgt
      781 ccccgaacaa accgagatcg atcacgaaag aggtttgatt tttgattctc atgatgaaca
      841 cgcagccata ttctacgcac aaaaaggccc ggacatcgtg gggtctatca gggttatcac
      901 tgatttgcct caacaacttt ctgagcaggg aatgaatttg tatcaatgcc atgaactgat
      961 cacgaggtta cagcctcatc aaacctcagt cacggatatg tttgtcctcg acccaaaggt
     1021 acggggtcag accctcgttt cgttgttgtg gtctgc
//
LOCUS       DATDUN010000209         1412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_94549, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000209 DATDUN010000000
VERSION     DATDUN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94549"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1412)
                     /locus_tag="VJC18_02780"
     CDS             complement(<1..>1412)
                     /locus_tag="VJC18_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44336.1"
                     /translation="SAAERKFQTGSGSLVLHGVKGLAVGFRAVGSAGLIFAYQTLSSS
                     LYGQWVGRILGIHNNEPQREVLVVQMTGWRGKTARRAMTLARLMGIEAYIGDLIEYRP
                     VKLSLAAVPKKYPVRFVVNLSPDNPFGLDFTAVSEERHEIVPSPTRVPALLQGVLEER
                     FENDIVAMAAAIEQPPETLNNFMTGQLPKDYRVVRQIAESLFADNPSRFVAAWVDDFL
                     DLMEGTYSNHPWGPDSAVLGLMYMIRRRSLFAIYSAEGVTRFENGIELTAIETAILGQ
                     MIRGEFVGESGHLLWNRELAQRIIADTVSDDDELIVQILDLATSEINTEAQKRQGRGH
                     QETTVLEEREVVRDGSEPALFPDHTKLSPQRQWALYATLNLPLSDLFKDWHKKKKHYQ
                     LTINALELLPLLLRGDTRIKLNRLNQFLRAYEPQPSASSSDSDRLPATRWNNTIHKSD
                     FVLGDLIAHEAEDIRTGVSF"
BASE COUNT          350 a          348 c          343 g          371 t
ORIGIN      
        1 aaaggaaacc cccgttctga tgtcttctgc ctcatgtgca atcaggtcac caagcacaaa
       61 atcagatttg tgaattgtgt tattccaacg ggtcgccggc aagcggtcag aatcagaaga
      121 ggaggcagac ggctgtggtt catatgcgcg gagaaattga ttaagtctgt ttagttttat
      181 tctagtgtca ccacgcaaaa gcaagggcaa gagttctagc gcattgattg ttaactgata
      241 atgttttttt ttcttgtgcc aatctttaaa cagatcactc agtggtaaat tcaacgtggc
      301 atacagtgcc cattgtctct gcggacttaa ttttgtatgg tcgggaaaca gtgctggttc
      361 cgaaccatcg cgaacaacct cacgctcctc taggacagtg gtttcctgat gtccccggcc
      421 ttgtcttttt tgtgcctcgg tgttgatttc tgatgtggca aggtcaagaa tctggacgat
      481 caactcatca tcatcactga ccgtgtctgc gatgattcgc tgtgccagtt cgcggttcca
      541 taacagatgg cctgattcgc caacaaattc tccacgaatc atttggccca aaattgctgt
      601 ttcgatggca gttaattcaa tgccattttc gaatcgtgtg accccttcag cagaataaat
      661 ggcaaaaagg gaacggcgtc tgatcatgta catcagcccg agtacggcgc tgtcaggtcc
      721 ccaaggatgg tttgagtagg ttccctccat gagatcaaga aagtcatcca cccaggcggc
      781 aacaaatctt gagggattgt ctgcaaaaag actttcggcg atctgtcgca ccactcgata
      841 atccttgggc agctggcctg tcataaaatt attaagtgtc tctgggggtt gctctattgc
      901 tgcggccata gcgacaatat cattttcgaa ccgctcttct aaaacacctt gcagtagggc
      961 aggcactctg gttggagagg gaacgatctc atgtcgttct tcagacacgg cggtaaaatc
     1021 aagaccaaag gggttatctg gtgataggtt gacaacaaaa cgaacaggat attttttggg
     1081 cacggccgcc aaagacaatt ttacgggacg gtattcaatg aggtcgccga tataggcctc
     1141 tatccccatg agtctggcca gcgtcatggc cctgcgagcg gtttttcctc tccagccggt
     1201 catctgcaca accaacacct cgcgttgtgg ctcgttatta tgaattccca aaatccttcc
     1261 cacccattga ccgtataacg aggatgacag tgtttggtag gcaaagatga gccctgcgga
     1321 gcccaccgcg cggaacccca cggccaaacc tttgacaccg tgcaggacca gcgatcctga
     1381 acctgtttga aatttgcgtt ctgccgcgga at
//
LOCUS       DATDUN010000210         6703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_10933, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000210 DATDUN010000000
VERSION     DATDUN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10933"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /gene="cas5"
                     /locus_tag="VJC18_02785"
     CDS             complement(<1..151)
                     /gene="cas5"
                     /locus_tag="VJC18_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HLD44337.1"
                     /translation="MDYLVFQLRAPLSAWGETAVGEYRPTSNYPSNSALLGLLAAALG
                     IDREDE"
     gene            complement(154..1209)
                     /gene="cas7e"
                     /locus_tag="VJC18_02790"
     CDS             complement(154..1209)
                     /gene="cas7e"
                     /locus_tag="VJC18_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HLD44338.1"
                     /translation="MSRFYQIHLLVSYPPSNLNRDDTGRPKTAIVGDANRLRISSQSL
                     KRAWRTSEAFEEALSGHIGVRTKEMGVEIYQKLIEGKISEKNARDWAKSIASQFGKLK
                     SDKKTEGNEDLHVEQLVHLGTEERAAITCLIEKLVVANIAPTEEDLKLLRKPRQAVDI
                     AMFGRMLASSPAYNIEAAVQVAHAITIHKVAVEDDYFTAVDDLNKGDVDRGSAHIGEA
                     GFGAGVFYLYVCINRELLCDNLGGDVGLTSKALEALMHAITKVSPTGKQNSFASRAYS
                     AYVLVEKGDQQPRSLAQAFLKPVTGTEMLDRAVKELNKRIDNFDRIYGPCADKRCSFN
                     VETGEGSLKDLVTFIKD"
     gene            complement(1230..1736)
                     /gene="casB"
                     /locus_tag="VJC18_02795"
     CDS             complement(1230..1736)
                     /gene="casB"
                     /locus_tag="VJC18_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HLD44339.1"
                     /translation="MSEKISFHQDTALGQVLSRWWKGLDKNRGPRAELRRAHDLTAVA
                     LTSAYQHFYRQMVVAGWVSSASDDWQNERLTAIAGLLAHVTSEDARTLPHAMSDGDQP
                     AFSELRFRRLLESQTIDEAFISLRRAIPMIGDKANIHQLATDVLYWGDNVKKRWAYDY
                     RWPDKKQS"
     gene            complement(1757..3391)
                     /gene="casA"
                     /locus_tag="VJC18_02800"
     CDS             complement(1757..3391)
                     /gene="casA"
                     /locus_tag="VJC18_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HLD44340.1"
                     /translation="MNLLDEAWIPVRRSNGKLDWISPYQMTESDLVALAANRPDFNGA
                     LIQFLIGLVQTTTPMDSQAEWERWNTNPPNIEELKKWFAPVREAFVFDGDGSRFMQDF
                     DLRTQETKDDEFNEIGALLIESPGDQTLKLNKDHFVKRNRTSALCPHCAITALFTLQT
                     NAPSGGNGHFTSIRGGGPLTTLIMPGQSSSLWQIVWLNVREKTQFNTSHGNADLKKPQ
                     YIFPWLGDMKTIQPDGGKTTPIQVHPAHVFWGMPRRIRLDQENVTKGACDICGRLSDK
                     LMTRYLTIARGFDYKGGWRHPLSPYREEKQGEMFAVHPQPDGIGYKHWLSWILGQQTD
                     KKKILPASIVSHVIDSHRLASHYRLWVFGYDMDNMKARCWYESILPLYGVTDKNIDGR
                     RLIQREIAKWLESAEYVVYLLRSNVKSAWFGDDAEVRGNLSFIDVFFWSQTEPDFYHQ
                     LIALIEQARDEENDPTLLPMREAWREVLIKVALHLFDADLVGVAPIQQQNPRRMAEAY
                     KRLRMSLYGDKLKALLGLPVEKKEKKIKKSAKNVAA"
     gene            complement(3388..6165)
                     /gene="cas3"
                     /locus_tag="VJC18_02805"
     CDS             complement(3388..6165)
                     /gene="cas3"
                     /locus_tag="VJC18_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HLD44341.1"
                     /translation="MKSFYKYWGKAKTADAGVLSWHLLPYHCLDVAAVGVEYLNQNTK
                     LCDFFCDRLACNQQQFLNWAGLLLSLHDLGKFSEAFQAQKPDLFLLLQQRAPLPEKQY
                     QERHDSLGQWLWRDWFSEQVSVKKWLGLSSQTTMTGMDYWLRSVTGHHGQPPKVENIA
                     GASDDFFTKADKKSVLSFFESIYGIFLSEGDESILTKHFPEEFEHISQELSWWFAGVV
                     VLADWIGSNTDYFPYQKNEIELAEYWGVAREQARKALLQSGVLPITIKKNLKFNDLFP
                     KIVTPTPLQEWSNKVKISNGPQIYLLEDMTGAGKTEAALMLAYRLMVAGNASGFYVAL
                     PTMATANAMYERIANIYGLLFAGNSNLALVHGSRELVDKFASTIIPKSKSERDDTQDD
                     ETATARCAAWLADHNKRALLAQAGVGTIDQILLGVLHSKHQSLRLLGLFQKVLIVDEV
                     HACDSYMQGVLEVLLEFHARAGGSAILVSATLPNKMKQTLLKSYARGCNQICPTITQT
                     AYPLATSWKCYEPTIVCEQPIAIRTEVSRQVRVEYLCERSDLEAKIENVLASGMCVCW
                     IRNTVSDAMDAYGVLSKKISRDNISLFHARFTLHDRLQKEDVIKELFGPASTAVKRAG
                     KLVIATQVIEQSLDVDFDLVVSDLAPIDRIIQRAGRLRRHVRDKQGNRILDCGAKDQR
                     GSAVLFVYGPAWDENPKPTWFKQQLPKAAAVYSDHAQLWLTAKALQKGQYSMPDSARD
                     LIENVFDENMQIPDGLQKCNLTAQGANMASASVAKNNTLTFATGYRRGDVMDWWSEAK
                     TPSRLGEASNNVILARWKNNKIVPWVNREHAWAYSTVRVAERLIAKAHLPTDQDELLE
                     YERVLEAFPNKGKWLTLLPLKDNGDGIWKAQAWTVESKGHPAQLLTWQYDADHGLRRW
                     DDNLQTPEETE"
     gene            complement(6208..>6703)
                     /locus_tag="VJC18_02810"
     CDS             complement(6208..>6703)
                     /locus_tag="VJC18_02810"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HLD44342.1"
                     /translation="AHERIGFEKLLKEYEHKGIQKKPLLISETFDLKPSDAALLKEYT
                     DELNAFGFEIDFFGGNTFVLKAVPQLPGSQIDIAMFVGDLVEDIKETGEMVSLKTRFH
                     AVLARIACHAQIRAGHHLEPQEIQQLLSDLDQHQFTDFCPHGRPVCVTVTKDEIEKWF
                     KRVV"
BASE COUNT         1795 a         1670 c         1342 g         1896 t
ORIGIN      
        1 gttcgtcttc acgatctatc cccaaggccg ccgccagcaa acccagcaaa gctgagttgc
       61 tggggtaatt tgaggtaggt cgatattcac ccacagcggt ttcgccccaa gccgacaaag
      121 gcgcccgcag ttgaaagaca agatagtcca tagttaatcc tttatgaatg taaccagatc
      181 tttcaagcta ccttcccctg tttcaacatt aaaactacag cgtttgtcgg cgcatggccc
      241 ataaatccgg tcaaaattat caatgcgttt attcaactcc ttaacggcac gatccaacat
      301 ttccgtgcct gtaacaggtt ttaagaatgc ctgtgccagt gaacgtggtt gttgatctcc
      361 tttttctacc aatacgtatg ccgaataggc gcgagaggca aaactgtttt gtttgccggt
      421 gggtgatact ttcgtgatcg catgcatcaa ggcctctaag gccttactgg tgagcccaac
      481 atcaccccca agattgtcac aaagcagttc gcgattgatg cagacgtaaa gataaaatac
      541 accagcacca aaaccggctt ctccaatatg agcagaaccc cgatcaacgt cacctttatt
      601 aagatcatcg acggcggtaa aatagtcatc ttctaccgcc actttgtgaa tggtaattgc
      661 atgagccacc tggacggcag cttcaatatt atatgctggt gaggaggcca acatgcggcc
      721 aaacatggca atatctacag cttgtctggg tttacgaagt agttttaagt cttcctctgt
      781 tggcgcaatg tttgccacca ccaatttttc tatcaagcac gtgatagcag cacgttcttc
      841 tgtgccaagg tgcactaact gctcaacatg caaatcttca ttaccttctg tctttttgtc
      901 agatttcaat ttaccaaatt ggcttgcaat agacttggcc caatcacggg catttttttc
      961 tgaaatttta ccctcaataa gcttctgata aatttcgaca cccatttctt tggtacgaac
     1021 accaatatgg ccactcaatg cctcttcaaa tgcctctgat gttctccacg cccgctttaa
     1081 gctctgtgat gaaatccgca ggcgattggc atcgccaaca atagcggttt ttggcctccc
     1141 tgtatcatcg cgattaagat tcgatggcgg atagctgaca agtaaatgaa tttgataaaa
     1201 tcgtgacatg gtattctcct tatttaaagt taagattgtt ttttgtcagg ccaacggtaa
     1261 tcgtaggccc atcttttttt aacgttatcg ccccaatata aaacatcagt agccaattga
     1321 tgaatattgg ctttatcgcc aatcatggga attgcccgac gtaagctgat aaacgcctca
     1381 tctatggttt gtgattccag aagtcgcctg aaacgcaact cagaaaatgc aggttgatca
     1441 ccatctgaca tggcatgtgg taaagtacgc gcatcttcac ttgtaacatg agccaacaag
     1501 ccggcaatgg ctgtaagtcg ctcattttgc cagtcatccg acgcgcttga aacccagcct
     1561 gccacgacca tttgccgata aaaatgctga taagcactgg tgagtgctac agcagttaaa
     1621 tcatgtgccc ttctgagttc tgcacgggga cccctgtttt tatctaaacc cttccaccag
     1681 cgtgacagca cttgtcctaa tgctgtgtct tgatgaaatg atattttttc actcataatg
     1741 ttactccttc aacaattcat gctgccacat ttttggcaga tttcttgatt ttcttttcct
     1801 ttttttccac cggcaagcca agaagggctt ttaatttatc accatacaaa ctcatgcgta
     1861 atcgcttgta ggcttcggcc atgcggcgcg gattttgttg ttggatagga gccacgccca
     1921 ctaggtcagc atcgaacagg tgtaaggcaa ccttgattaa aacttcacgc caagcctcac
     1981 gcattggcaa taaggttggg tcattttctt catcgcgcgc ctgctcaatg agcgcaatga
     2041 gttgatgata aaaatcaggt tcggtttgtg accaaaaaaa gacatcgata aaactcaagt
     2101 tgccacgcac ttctgcatca tcaccaaacc acgctgactt gacattggat cgtagtaaat
     2161 aaacgacata ttcggcagac tcaagccact tggcgatttc acgttgaatt aggcgccgtc
     2221 catcaatgtt tttgtcagtt acgccgtaaa gaggtaagat tgattcgtac cagcaacgtg
     2281 ccttcatatt atccatatcg taaccaaata cccataatct gtagtgactc gccaaacgat
     2341 ggctgtcgat gacatgagaa acaatcgatg ctggtaatat tttcttctta tctgtctgtt
     2401 gacccagtat ccagctcaac caatgtttat aaccaatacc gtctggctgt gggtgcactg
     2461 caaacatctc gccctgtttt tcttcacgat agggagacaa aggatgacgc catcccccct
     2521 tataatcaaa gcctcgggct attgttaaat aacgtgtcat taatttgtct gacaacctac
     2581 cacagatatc acatgccccc tttgtaacat tttcttggtc caagcgtatg cgtctaggca
     2641 taccccaaaa cacatgagca ggatgcactt gtattggcgt tgttttaccc ccatctggtt
     2701 ggatggtttt catatctccc aaccaaggaa atatgtattg tggctttttt aaatcagcat
     2761 tgccatgaga ggtgttaaat tgcgtttttt cacgaacgtt caaccaaact atttgccaca
     2821 atgatgaaga ctggcctggc ataatcaatg ttgtcaatgg cccaccacca cgaattgatg
     2881 taaaatgccc attgccacca ctcggtgcat ttgtttgaag ggtaaacaat gcggttattg
     2941 cgcaatgagg acataaagct gaggtgcgat ttcttttaac aaagtgatct ttgtttagtt
     3001 tgagtgtctg atcgcctggc gattcgatca acaaagcccc aatttcatta aattcgtcgt
     3061 ctttggtttc ttgtgtacgc aaatcaaaat cctgcataaa ccgcgagcca tccccatcaa
     3121 aaacaaacgc ttctcgcaca ggcgcaaacc attttttgag ttcctcgata tttggtggat
     3181 ttgtattcca cctctcccac tcggcctggc tgtccatggg tgttgtggtt tgtacaagtc
     3241 cgattagaaa ttgtatcaac gccccattaa aatctggtcg gttggcagcg agcgcaacaa
     3301 gatcagactc tgtcatctga tacggtgaaa tccaatccag tttaccattt gatcggcgca
     3361 cgggaatcca ggcttcgtct aataaattca ttctgtttcc tccggcgttt gcagattgtc
     3421 gtcccatcgt ctcaaaccat ggtctgcatc atattgccaa gttaataatt gtgcaggatg
     3481 ccctttagat tctaccgtcc aagcctgtgc tttccaaata ccatcaccat tatctttgag
     3541 cggcaacaga gttaaccatt ttcctttatt tggaaaggct tccaaaacgc gttcatactc
     3601 taacagctca tcttgatccg tcggcaaatg ggctttggct attaacctct cggcaacacg
     3661 cacggtacta taggcccaag catgttcacg attaacccac ggcacgattt tgttgttttt
     3721 ccacctggct aaaataacgt tattgcttgc ctcacccaaa cgcgaaggtg ttttagcttc
     3781 actccaccag tccataacat caccccggcg atagcccgtt gcaaaagtca gcgtgttgtt
     3841 tttagcaacg ctcgcactcg ccatatttgc cccttgcgct gtcaaattac atttttgcaa
     3901 accatctgga atttgcatgt tttcatcgaa tacattttca atcaaatctc tggcagagtc
     3961 aggcattgaa tattgacctt tttgcaaggc tttggcagtc aaccaaagtt gtgcatgatc
     4021 tgaataaact gcggccgctt ttggcaattg ttgcttaaac caggttggct tgggattttc
     4081 atcccaagcg ggaccataaa caaacaaaac agctgaacca cgttgatcct tggcaccaca
     4141 atccaaaatt ctatttccct gtttgtccct gacatgacgt cgcaaacggc ctgcgcgttg
     4201 tataattcga tcaatgggtg ccaaatcact taccaccaaa tcaaaatcca catcgagcga
     4261 ttgttcaatc acctgcgtgg caatgaccaa ctttccagcc cgcttgaccg ctgtactggc
     4321 tggtccaaag agttctttta tgacatcctc tttttgtaag cggtcatgca aagtgaaacg
     4381 tgcgtgaaac agactgatat tatcacgcga gattttttta gaaagaactc cataagcatc
     4441 catggcgtca gataccgtat tacgtatcca gcaaacacac atgccagacg ctaacacgtt
     4501 ctcaattttt gcttccaagt cactgcgttc gcaaagatat tctactcgca cctgacggga
     4561 gacttctgtt cgtattgcaa ttggctgttc acagacaatt gtgggttcat aacatttcca
     4621 tgaagtagcc aaaggatacg ctgtctgggt gatcgtcgga cagatttgat tgcaaccacg
     4681 tgcataggat tttagcagtg tctgcttcat tttattgggt aaagtggccg aaaccaaaat
     4741 tgcgctaccc ccagcacggg catgaaactc taaaagaacc tctaaaacac cttgcatata
     4801 ggagtcacaa gcatgcacct catcaacaat aagcaccttt tgaaataatc caagtaatcg
     4861 caatgactga tgtttgctgt gcaaaactcc taacaggatt tgatcgattg tcccaacccc
     4921 agcttgggca agcaatgccc ttttattatg atctgcaagc caagccgcgc aacgtgccgt
     4981 agctgtttcg tcgtcttgtg tgtcatcacg ctctgatttt gattttggaa taattgtgct
     5041 ggcaaatttg tccaccagct cacgactgcc gtgcaccaaa gccaggttcg aattgcctgc
     5101 aaaaagcagt ccgtagatat ttgcaattct ctcatacatg gcattagccg ttgccatggt
     5161 gggaagagcc acataaaaac ctgacgcgtt gcctgcgacc atcaaacgat atgccagcat
     5221 caaagcggcc tcggttttgc ctgctcctgt catatcttct aaaagataaa tctgtggacc
     5281 atttgaaatt ttaactttgt tcgaccactc ctgcaatggg gttggtgtca caatttttgg
     5341 aaacaaatca ttaaatttta gatttttctt gattgtaatt ggcaaaacac ctgactgtaa
     5401 aagggctttt ctggcttgct cacgagcaac gccccaatat tccgcaagtt caatttcatt
     5461 tttttggtaa ggaaagtaat ctgtatttga acctatccag tctgcaagca ccacaactcc
     5521 tgcaaaccac catgaaagtt cctgactgat gtgttcaaat tcctcaggga aatgtttggt
     5581 gagaatagat tcgtcaccct cgcttaaaaa aatcccataa atgctttcaa aaaacgaaag
     5641 cactgatttt ttatcagcct ttgtaaaaaa atcgtcactg gcacctgcaa tattctcaac
     5701 tttggggggc tggccgtgat gtcctgtgac ggaacgcaac caataatcca tccctgtcat
     5761 ggtcgtctgg gaactcaatc caagccattt tttgacactg acctgctcag aaaaccagtc
     5821 acgccacaac cactgtccaa gtgaatcatg tcgctcctga tactgttttt ctgggagagg
     5881 tgctcgttgt tgcaggagca aaaataaatc aggtttttgt gcttgaaagg cttcgctgaa
     5941 tttacccaag tcatgcaaag acaacaacag accagcccaa tttaaaaatt gttgctgatt
     6001 acatgccaaa cgatcacaaa agaaatcaca cagttttgta ttttggttaa gatattcgac
     6061 accaacagcc gccacatcca gacaatgata tggcaacaaa tgccacgaca ggacaccagc
     6121 gtcagccgtt tttgccttcc cccaatattt gtaaaaactt ttcactcgct tcccctcgca
     6181 ttcacctgct ccccaataac cactccatca cacaaccctc ttgaaccact tttcaatctc
     6241 gtccttcgtg actgtgacgc agacgggacg accgtgggga cagaaatcgg tgaactgatg
     6301 ttgatcgaga tcactcagga gttgttggat ttcctgcggc tccagatggt gccctgccct
     6361 gatctgcgcg tgacaggcaa tacgcgccaa aaccgcgtga aatctggttt tgagagaaac
     6421 catctctccc gtctccttga tatcctccac cagatcaccc acaaacatgg cgatgtcgat
     6481 ttgactgccg ggtaactggg gaaccgcttt gaggacaaac gtgttgcctc caaaaaaatc
     6541 gatttcaaaa ccaaacgcgt tcagctcatc ggtgtactct ttaagcaaag cggcatcact
     6601 gggcttgaga tcaaaggtct cggaaatgag aagaggtttc ttttggatcc ccttgtgttc
     6661 atattccttc agcagttttt cgaatccaat gcgttcgtgc gcc
//
LOCUS       DATDUN010000211         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_466364, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000211 DATDUN010000000
VERSION     DATDUN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466364"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VJC18_02815"
     CDS             complement(<1..344)
                     /locus_tag="VJC18_02815"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HLD44343.1"
                     /translation="MKKTNLFLALLFLSCLHCLGPQKTLPEYSREVARVDDAVIEEEL
                     LRFKMRLEINKLPDGIFQDMNPTLLVQNPDFRELLQSTLNKMIDDQVVIAYGKKKGVA
                     ISLEELAEKLNE"
     gene            398..1048
                     /gene="coaBC"
                     /locus_tag="VJC18_02820"
     CDS             398..1048
                     /gene="coaBC"
                     /locus_tag="VJC18_02820"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HLD44344.1"
                     /translation="MPKPKKILITAGPTQEPIDPIRYLSNHSSGKMGYALARACVKAG
                     HQVVLISGPTNLVVPEGVVFVAVTTAREMYRQAISVFPSCDLVFKVAAVADYRVKKKS
                     LRKIKKTGETMLLELVRNPDILKKLGQLKKPHQTLIGFAAETHKGAAYAQKKLRGKNL
                     DWIALNEINKHNVGFGRDDNEVTLISSGGRKVIIPKQGKDAVAKIILETVYPTATP"
     gene            complement(1011..>2281)
                     /locus_tag="VJC18_02825"
     CDS             complement(1011..>2281)
                     /locus_tag="VJC18_02825"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HLD44345.1"
                     /translation="AKQPIVINTINPQLNELTEQEALELGMVPLELNIKEKDKVELKK
                     VYLGLALYYRDRRESIPVAAQIPTLEYKLNLALLKLTQDKSPRVGLLIGQDKNQYSFL
                     DRFISEIGTPVEIDPQTTNLAAYDLDSFLLVAPDHLDKKILSQIDALIDSGLSVAIFS
                     GNTSVNDNLQASIIATGLDDWLEDKGLMISQKLLVDILQNEQALFQQGALQVYRDYPF
                     WIKTMRADVNPEHPITNSIEEILFPWTNALLIAPQDNPKWEIDELVRSSTNSFLQPEE
                     TTNVAPDYVQTMTQQPTLANYPLSVSLTNKTNPHAGKIYVTSTHNLIQDIFLEQAHAN
                     VLFLSNMLEHATWGNKLISLRTRGKTTRPLKELSPQTLLSIKWGHLAGIPLLAILFGL
                     MVNKLWSVKRNSSIKQISVKGSQSDTLSPK"
BASE COUNT          545 a          507 c          631 g          598 t
ORIGIN      
        1 ctctcgttaa gtttttcggc cagttcctca aggctgatgg caaccccctt ttttttgccg
       61 taagcgatga ccacctgatc atcaatcatc ttattgagag tggactgcaa aagttcgcga
      121 aaatccgggt tttggaccag cagggtggga ttcatgtcct ggaagatgcc gtcaggcaat
      181 ttgttgatct caagcctcat cttgaatcgc aacaactctt cctcaatcac ggcgtcatcc
      241 acgcgggcaa cctcgcggga gtactcaggg agtgtctttt gtggacccag gcagtgcaga
      301 caggataaaa acaaaagggc aagaaagagg tttgtttttt tcatttgcca aaaaaacccg
      361 aaaccgttta aagtcccggc cgtccgcaaa attcattatg ccaaaaccaa aaaaaattct
      421 catcacagcc ggtcccacgc aggaacccat agatccgatc cgctatttgt caaatcattc
      481 cagcgggaag atgggatacg ctttggcgcg agcctgtgtc aaggccggac atcaggtggt
      541 tttgatttcg ggacccacga atttggttgt gccagaaggg gtggtgtttg ttgctgtcac
      601 aacggctcgc gaaatgtatc gtcaggcgat cagcgttttt ccatcgtgtg atctggtctt
      661 caaggttgcg gctgtggctg actatcgtgt gaaaaaaaaa tccctcagga aaatcaaaaa
      721 aaccggtgag acgatgttgt tggaattggt gaggaacccg gacattctca aaaaactggg
      781 gcagttaaaa aaaccgcatc agaccctgat tggttttgcc gccgaaacgc ataagggtgc
      841 ggcgtacgcc cagaaaaaac tcaggggaaa gaacctcgac tggatcgcgt taaacgagat
      901 caacaaacac aatgttggat tcggtcgcga tgacaacgag gtgacgctca tctcaagcgg
      961 tgggcgcaaa gtcatcattc ccaaacaggg caaagacgcc gtggcgaaaa ttattttgga
     1021 gacagtgtat ccgactgcga ccccttaaca gatatttgtt tgatgctgct gtttcgtttt
     1081 accgaccaca gtttgttgac catgagtcca aacaggatcg ccagcagggg gattcctgcc
     1141 agatgtcccc acttgatgct gaggagggtt tgaggtgaca gctctttgag cgggcgggtg
     1201 gttttgccgc gcgtgcgcag ggagatcagt ttgtttcccc acgtggcgtg ctccagcatg
     1261 ttcgataaga acagcacatt ggcgtgagct tgttccaaaa aaatatcttg aatcaaattg
     1321 tgcgtgctgg tcacgtagat ttttcctgcg tgagggtttg ttttattcgt gagagagaca
     1381 gacaggggat aattggccag cgtgggttgt tgggtcatgg tttgaacata gtctggtgcg
     1441 acgttggtcg tctcctcggg ttgcaaaaaa gaatttgtcg acgagcgcac caattcgtca
     1501 atttcccatt ttggattatc ttgtggggct atgagcaggg cgttggtcca ggggaacagg
     1561 atttcctcga tggaattggt gatggggtgc tcaggattca cgtcggcccg catggttttg
     1621 atccaaaaag ggtagtctct gtaaacctgc aaggctccct gttgaaaaag agcctgttca
     1681 ttttgcagga tgtcgaccag gagtttttgg gaaatcatca gtcctttgtc ttcgagccag
     1741 tcatccaggc ccgtggcgat gatggaggct tgcaagttat cgttgacact ggtgttgcca
     1801 gagaagattg ccaccgagag cccactgtcg atcaaagcat cgatttgaga caggattttt
     1861 ttgtcaaggt ggtcgggagc gaccagcaaa aagctgtcga ggtcgtaggc ggcaagattg
     1921 gttgtctgtg ggtcgatctc cacgggagtt ccgatttcgg aaatgaacct atcgagaaaa
     1981 ctgtattgat ttttgtcctg gcctatcagc agtcccacac gcggcgactt gtcctgcgtg
     2041 agtttgagca aggccagatt gagcttgtat tccagtgtgg ggatttgcgc ggcgacggga
     2101 atgctttcgc gacgatcgcg gtaatagagt gccagcccca gataaacttt tttgagctcg
     2161 actttgtctt tttccttgat gttgagttcc agcggaacca tccccaactc aagcgcttcc
     2221 tgttcggtca attcattgag ttgtgggttg atggtgttga tgacgatggg ctgttttgcc
     2281 t
//
LOCUS       DATDUN010000212         3246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_175551, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000212 DATDUN010000000
VERSION     DATDUN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175551"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VJC18_02830"
     CDS             complement(<1..243)
                     /locus_tag="VJC18_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HLD44346.1"
                     /translation="MTSDEIFNKKISEKLNNNAEKVKSINAVYTFELTGDTTESWTID
                     LTKAGDFISQGAAATPNCTVSIKLEDLADIVEKKINP"
     gene            complement(332..754)
                     /locus_tag="VJC18_02835"
     CDS             complement(332..754)
                     /locus_tag="VJC18_02835"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HLD44347.1"
                     /translation="MNFRPSAISLITRLAESTPALILLGTLTLGWFVTPSDLPQETLC
                     TFRRLFHFDCPGCGLTRAFLLLARGHLIDAITYNAAAPILYILFGIITITVIGRQYKP
                     SFGDNPFWSQTRWFLAIMALAIMMIHWVIKVAIFFWML"
     gene            complement(957..2744)
                     /locus_tag="VJC18_02840"
     CDS             complement(957..2744)
                     /locus_tag="VJC18_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HLD44348.1"
                     /translation="MEPNNLIALFNATVAARPSKPSMQYLFNGSWNTLTWSEVKDRVM
                     KIAGGLRQRGIQKGDRVAIISKTRHEWTLADLGIMAASGVTVPIYESNTSTQAAYILE
                     NSQAKIVIVENEGQLQKILPVKNSLPHLTHIVHIDNITSSPTDRLCSLDDVMNSDLTE
                     VQFVYDEALASLNLDHDASFVYTSGTTGEPKGAILTHGNFVAEAKGALAICPIGEDFT
                     GLLFLPLAHILGRVTQFFHIHVGHTQCYAESIDRLIDNIATVKPHFMVSVPRIFEKIH
                     ARTLQNVENSPPLKQHIFRWALKIGEQRSQRLLTKQPLPVCMKLKYFLAHHLVFKKLH
                     DKMGGRIRFFISGGAPLSADLALFFHAFGFTILEGYGLTETTAGLSFNRSHFIKFGTV
                     GQPIEDAVAVKIAGDGEICVRGKIVFKGYFNNPQATREAIDEEGWFHTGDIGEFDTDG
                     FLKITDRKKDIIVTAGGKNIAPQSIENHMITDPFISQFIVHGDKRKFLSALVVLDRDQ
                     VLRFAKEHDIPFASYEELVTHHKLQEFIKNRIEQKNSDLASYETIKKFAILPNDFSIE
                     SGELTPTLKLKRKVIYQKYTSVLDGFYKE"
     gene            complement(2787..3149)
                     /locus_tag="VJC18_02845"
     CDS             complement(2787..3149)
                     /locus_tag="VJC18_02845"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HLD44349.1"
                     /translation="MLSKKDIGNQGEDTAATYLRSKGYRIIEQNVRTRYGEVDLIARQ
                     GNKIFFVEIKTRTGPTYGQALDAITPDKIRKMTNTAQALINKHKEWQTLIPFLSVLAI
                     DYDLNGDPHIEFLPNAVE"
BASE COUNT          733 a          727 c          908 g          878 t
ORIGIN      
        1 cgggttgatt ttcttttcga cgatgtcggc caggtcttcg agcttgatgg aaacagtaca
       61 gttaggtgtg gccgcggcgc cctggctgat gaagtcgccc gccttggtca aatcgatcgt
      121 ccagctctct gtggtgtcgc cggtgagttc aaaggtgtaa acggcattga tgcttttgac
      181 tttttccgcg ttgttgttga gcttttctga aatttttttg ttgaagattt cgtcactggt
      241 catggttgat ctcctgtttg agggacgcca gggattgacg tcgccaatat tatggttgct
      301 ggtaacacag agctttttta caggcaagtg attacaacat ccaaaagaag atagcgacct
      361 tgatcaccca atgaatcatc atgatcgcca aggccataat ggccagaaac cagcgtgttt
      421 gcgaccagaa cgggttgtca ccaaagctgg gtttatattg tctcccgatc acggtgatgg
      481 tgatgatccc aaaaagaata tacaagatgg gggcggcggc attgtatgtg atggcgtcaa
      541 tcagatggcc tcgcgccaat agcaaaaaag cgcgcgtgag accgcatccg gggcaatcaa
      601 agtgaaacag gcgacggaag gtgcagaggg tttcctgcgg cagatcggaa ggggtcacaa
      661 accagcccaa cgtgagggtg ccaagcagaa tcaaggcggg tgtgctctcg gctaaacggg
      721 tgatcaatga tatggctgaa ggcctgaagt tcattcgaag tcccaagttg aatctcgaaa
      781 tctgttttgt catctttgtc atccctgcgc aggcagggat ccagaatata tgtaaatatg
      841 gattccagca tttcagccgt tggactgatc ccgtttgcgg gacgctggaa tgacaaccac
      901 ttcgaaattc ctttgcctgt cctgagccag ccgaaggatt ccttgttcga tattcgttac
      961 tctttataaa atccatccag aaccgaggtg tatttttgat agatcacctt ccttttgagc
     1021 ttgagggtgg gggtgagttc acctgactcg atggaaaagt cattgggcag gatcgcgaat
     1081 tttttgatgg tctcgtaact ggcgaggtcg ctgttcttct gctcaatcct gttcttaatg
     1141 aactcctgaa gcttgtggtg agtgaccagt tcctcgtaag aagcaaacgg aatgtcatgc
     1201 tctttggcaa agcgcaggac ctgatcgcga tccagtacga ccagagcaga caggaatttg
     1261 cgtttgtcac cgtggacgat gaattgcgag atgaacggat cggtgatcat gtgattttca
     1321 atactttgcg gcgctatgtt cttgccgcca gccgtgacga tgatgtcttt tttgcgatca
     1381 gtgattttta aaaaaccatc cgtatcaaac tctccaatgt ccccggtgtg aaaccagccc
     1441 tcttcgtcga tggcctcacg cgtggcctgg ggattgttga aatacccttt gaacacgatc
     1501 ttgccgcgca cgcaaatttc gccatccccg gcaattttga ccgcgacggc atcttcaatc
     1561 ggctggccaa ccgtaccaaa tttaatgaag tgacttcggt taaaggagag ccctgctgtg
     1621 gtttcggtca ggccatagcc ctcgagaatg gtaaaaccga aagcgtgaaa aaagagggcc
     1681 aggtcggctg agagtggggc gccccccgat ataaaaaacc gaatgcgtcc gcccatttta
     1741 tcgtgtaatt ttttgaaaac caggtgatgt gccaaaaagt atttgagttt catgcaaacg
     1801 ggcaggggtt gtttggtcaa gagtctttga gaccgctgtt ctccaatttt gagggcccag
     1861 cggaagatgt gttgttttag gggaggggag ttttctacat tttgcagggt gcgcgcgtga
     1921 attttttcga aaatgcgtgg cacactcacc ataaaatgtg gtttgacagt ggcaatgttg
     1981 tcgatcaacc ggtcaatact ctcggcataa cattgtgtgt gtcccacgtg gatgtgaaaa
     2041 aactgggtca cgcgtcccag aatgtgtgcc agcggcagaa acagcaaccc cgtgaaatcc
     2101 tctccgatgg ggcagatggc caaagcaccc tttgcctcgg ccacgaaatt cccgtgcgtc
     2161 aatatggcgc ccttgggttc tcctgtggtt cccgatgtgt aaacgaaaga ggcgtcatga
     2221 tcgaggttga gcgaggcgag agcctcgtcg taaacgaatt ggacttcggt taaatcacta
     2281 ttcatgacgt cgtccagaga acagaggcga tcggttgggg acgaagtgat gttgtcgatg
     2341 tgaacgatgt gtgtgaggtg gggcaaggaa ttttttacgg gtaggatttt ttgcagttgt
     2401 ccctcgtttt cgacgattac tatttttgct tgggaatttt cgaggatgta cgcggcctgt
     2461 gtgctggtat tggattcgta gatcggtacg gtcacaccgc tggcggccat gatccccaga
     2521 tcggccagag tccactcatg gcgggttttg ctgatgatgg ccacgcggtc acccttttga
     2581 atgccgcgtt gtctgaggcc gcccgcgatt ttcatcaccc ggtccttgac ctcgctccaa
     2641 gtgagtgtgt tccatgaccc gttaaagagg tactgcatgc tgggtttgga ggggcgcgcc
     2701 gcgacggtag cattaaacag ggcgatgaga ttatttggtt ccatgccgcg tttataggcg
     2761 acccgttttt tatggcaaga aaaaactcat tccaccgcat tgggtagaaa ttcgatgtgg
     2821 gggtcaccgt tgaggtcgta atcgatcgcc agaacggaca gaaaggggat gagggtctgc
     2881 cattctttgt gtttgttaat cagggcctgg gccgtgttgg tcattttgcg gattttgtcg
     2941 ggggtgatcg cgtccagcgc ttgaccatag gtgggaccgg tgcgtgtttt gatttcaaca
     3001 aaaaagattt tgtttccctg cctggcgatg agatcgactt cgccgtaacg ggttctcacg
     3061 ttctgttcga tgatccggta accctttgat cgcaagtacg tggcggcggt atcttcgccc
     3121 tgattgccga tgtctttttt ggagagcata tcaaacgtga atcgtggatc gtggatcgtg
     3181 gatcgaaatc tgttttgtct tccctgcgga agcagggatc catatcttca ttaattctgg
     3241 atccca
//
LOCUS       DATDUN010000213         1699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_175700, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000213 DATDUN010000000
VERSION     DATDUN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175700"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VJC18_02850"
     CDS             complement(<1..644)
                     /locus_tag="VJC18_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44350.1"
                     /translation="MCSLNIQTTPPSFPKPPIAVGMTGIAPLDHVRAIPTAWKMAGNS
                     HGDFGDLLGFFSAVRPKTRIAYAVRLNQKRQDRAAKKTIDDQLRAMRGPDYRLGMIPE
                     QLHSLEKVSDSYGSPLCIDRSSPPPQQIALARACVLQKTETVRRCYPVNIDANDFDLL
                     LSALQMLESAQASLGADAVTQGFDNYQRHRTLPQIGERVVTLEYRRWLDYLVRCL"
     gene            1391..>1699
                     /locus_tag="VJC18_02855"
     CDS             1391..>1699
                     /locus_tag="VJC18_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44351.1"
                     /translation="MSRLDSRPHFEVSQAFVQNLNEQTPQVGQYGAILHYLQRQLGGQ
                     GATGPFIAIDTDKIRMSSTEKREYNRRVIDGILEGKEASALMTVRLLPGTSLLYSGLR
                     L"
BASE COUNT          404 a          436 c          436 g          423 t
ORIGIN      
        1 agacaacgca caaggtaatc cagccatcgc ctgtattcca gagtaaccac gcgttcacca
       61 atctgtggga gtgtgcgatg acgttgataa ttgtcaaaac cttgcgtcac ggcatctgct
      121 cccaggcttg cctgtgccga ttcgagcatt tgcaacgcgc tcaggagaag gtcaaaatcg
      181 tttgcatcaa tattcacggg ataacatctg cggacagttt ctgttttttg caggacacaa
      241 gccctggcca aggcgatttg ttgtgggggc ggagatgagc gatcaataca caacgggctc
      301 ccatagctat cgctgacttt ctccagcgag tgcaattgct cggggatcat tcctaaacga
      361 taatctggcc cgcgcatagc ccttagttgg tcatcaatgg tttttttggc agccctgtct
      421 tgacgttttt ggttcaggcg cacggcgtag gcgatgcgtg ttttcggcct cactgctgag
      481 aaaaagccca acaggtcccc aaaatcccca tgcgagttgc ccgccatttt ccacgctgtc
      541 ggtatggcgc gaacgtggtc gaggggcgct attcctgtca tgccaacggc gattggcggt
      601 tttggaaatg atgggggcgt ggtctgtatg ttgagactgc acatgggaca actccttttt
      661 catctgaccc ctcttacttt acttatctca caggggcaca aaatgttgcc tggtaaaagt
      721 gattcaatcc ataaaccggt cacatttgtg accacaatgt gagttttagc acattatgtt
      781 gtcctcatca tcactgcttt tgccgatgat aggggtgtca ccaaagttta cgagggaact
      841 aattatgact atgcagataa ttcgtccgtt aacgacgaca acacctccat tggtcgcggg
      901 gcaaacaagc cttccaacag ccgcccccct ttcacccagt cccgcagggg tggagacgac
      961 acccccgccg cggcaaattg ccccaatcga cactgtgatg attcctgaca agccttcagc
     1021 ggggtggttg gtacgcatta taagatgctt ttcgagcaaa tgcccacgca cagcaccccc
     1081 tcagcccaaa caagccgcat tgccagaaat gtcaacccca acacctcggc ctgatactac
     1141 ggtttcgttg tgtataggag accaaactga aagaaatgac cccgcggagg tttcatctga
     1201 acaggtgatt gcccgagctc ttttggattg catgatgcaa tcgagtctca gggtttctgg
     1261 tgcgctgagt gctctgcctc caaccttgga gtgtgtgctt tgcattaata gaaacaccgg
     1321 cgaggttgtg tttcgtgaac ctcatgtatc attgccatgt actaccgaat ggtttcaggt
     1381 tcaggggagt ttgagtcgac tggatagtcg cccccacttt gaggtgtctc aagcattcgt
     1441 gcaaaacctt aatgagcaga ccccacaggt cggacaatat ggggccattc tccattatct
     1501 gcaaagacag cttggtggcc aaggggcgac agggcctttc atcgccatag atacggacaa
     1561 gatcaggatg tccagcacag aaaagaggga gtataacaga agagttatcg acgggatatt
     1621 ggaggggaaa gaagcttcgg cgctaatgac tgtacgactt cttcccggaa cctcgctcct
     1681 gtatagtggt ttgcggctt
//
LOCUS       DATDUN010000214         2570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_260104, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000214 DATDUN010000000
VERSION     DATDUN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260104"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VJC18_02860"
     CDS             <1..662
                     /locus_tag="VJC18_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HLD44352.1"
                     /translation="NPQRVIPRAVVLSILVSIVLYLLVHIMLIGSGANLAGSEAPLAD
                     ALTQLIGPYGAMIISLGAVVSMFGYCAGLALGTPRYITVLCEDGFLPKLGARQHARYG
                     TPYVAIIIFSLATFILTLVLNFDSLVDIAATVIVIQYLLTCSAIPVLRKKVPSSKNTY
                     TSPFGPLVPVLGILSSLLLISQIRWIELKWVAGTLFLGYAFSGIYCQIMKRLKTYSKP
                     C"
     gene            complement(673..2046)
                     /locus_tag="VJC18_02865"
     CDS             complement(673..2046)
                     /locus_tag="VJC18_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44353.1"
                     /translation="MSEYYRSKSNEIHQLLSENKQHPNVIIVEGPRQVGKTTAVQMCL
                     KENAIPHLELNLEKNLAARDQIDACRNFNDFTDFLKDDFGFEPDSHKVLFIDEAQESE
                     NLGGFVRGMKEDWQNTHVILTGSMMTRLFRHDVRYPVGRVTIVHIQSLSFVEYLRAKR
                     INPEIVTLIESAQLEKISPLRHKDLLQHVSEYLPIGGLPEVIKAHLKNENHEAVIRQI
                     FENYRQDFMRVFGEELAYLFENALRGVADHVGSLSKISQALSHQDVAYRKMPEIFTRL
                     ENWHMIYLSQQRGSFPEGTTLMHPKRYLFDLGLLNKLRTTGIPSLSVLENTESAHRKT
                     LGGIIENFVAFHLVNLGFDLCGFRKNAVGLEIDFVIKRGPVAIPLECKASLRSKDTHL
                     KGLKIYMREFEQKIGVLLSLAPFEIHKFSAGTIIRLPVYAIEALPLLLDRVCAINGGK
                     IDAQVRR"
     gene            complement(2213..>2570)
                     /locus_tag="VJC18_02870"
     CDS             complement(2213..>2570)
                     /locus_tag="VJC18_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44354.1"
                     /translation="THRKFFDLFIAHSGGIATDLVVAPGENPHPDDFLKNFVSGRLGK
                     APFVGGRFFLYSGDEDESWGIKMSLYVENAKQKITQAGGEVVECVREAHGKHMGYRLN
                     TAVHERSIRHFIRLTP"
BASE COUNT          670 a          618 c          521 g          761 t
ORIGIN      
        1 aaaatccaca acgggtgatc ccacgcgcag tcgtgctttc gattctggtg tccatcgttc
       61 tttatttgct cgtgcacatc atgttgatcg gcagtggcgc caatctggca ggttctgaag
      121 cacctttggc ggacgccctg acacaactca ttggccccta cggcgcgatg atcatctcac
      181 tgggcgctgt ggtatcgatg tttggttatt gtgccggtct agcgctgggc acgccccgct
      241 acatcactgt gttgtgtgag gacggttttt tgcccaagct tggtgccaga caacacgcac
      301 gctatggaac accgtacgtt gccatcatta ttttttctct ggccacattt attctgactc
      361 tggttttgaa ttttgattct ctggtggaca ttgcggccac cgtgattgtc attcagtatc
      421 ttttgacctg ttcggccata cctgtgctca gaaaaaaagt cccttcatca aaaaatacgt
      481 acacatctcc ttttggaccg ctcgtaccag tcttgggcat tttgtcttct ttgcttttga
      541 tatcacagat tcgctggatt gagctcaagt gggtcgccgg caccctgttc cttggttacg
      601 ccttttcggg aatttattgt cagatcatga aaaggcttaa aacctattcg aaaccctgct
      661 aagacaacat catcatcgcc taacctgagc atcgattttg cccccattaa tagcacacac
      721 tcgatccagc aatagcggca aagcctcgat ggcataaaca ggaagccgga tgattgtgcc
      781 tgcagaaaat ttgtgtatct caaacggagc caggctcaaa agaacaccaa ttttctgttc
      841 gaactcgcgc atgtatattt tgagtccctt cagatgagtg tctttcgaac gcagagaggc
      901 tttgcattcc agcggaatgg caacaggacc acgtttgatc acaaaatcaa tttcgagccc
      961 aacagcattt ttcctgaatc cacaaagatc aaatcccaaa ttgaccagat gaaaagccac
     1021 aaaattttct atgatgcccc ccaatgtttt tctatgagct gattcagtat tttcaagcac
     1081 cgatagactg ggaatgcccg ttgtcctcaa tttattgagg agccccaaat caaacagata
     1141 tcttttggga tgcatgagtg ttgtcccctc aggaaaacta cccctttgct gactcagata
     1201 aatcatgtgc cagttttcca gacgcgtaaa tatttctggc atttttcggt aggccacatc
     1261 ctgatgcgaa agcgcctgcg aaatcttgct gagggatccc acatgatccg caacacctcg
     1321 caaagcgttc tcaaaaaggt aagccagttc ttcaccaaaa acacgcatga aatcctgcct
     1381 gtaattttca aaaatttggc gaatcacggc ttcatgattt tcatttttca gatgagcttt
     1441 gatgacttcg ggcaaaccac caatggggag atactcgctc acgtgttgca ataaatcttt
     1501 atgccggaga ggtgatattt tttccagttg tgccgattca atcaacgtca caatttcggg
     1561 gttaattctt tttgcacgca gatattctac aaatgacaaa ctctggatat gaactatcgt
     1621 gacacgccca acaggatacc gcacatcgtg cctaaacaga cgtgtcatca tggagcccgt
     1681 caaaatgaca tgagtgtttt gccaatcctc tttcatgcct cgaacaaaac cgcccaggtt
     1741 ttcactttct tgcgcttcat cgatgaaaag aactttgtga ctatcgggtt cgaaaccaaa
     1801 atcatctttt agaaaatctg taaaatcatt gaagttacga caagcatcaa tttgatctct
     1861 agcggcaaga ttcttttcga gattcagttc caaatgaggt atcgcatttt ctttcaggca
     1921 catttgaacc gctgttgttt ttccaacctg acgaggccct tcaacgatga taacattggg
     1981 gtgttgctta ttttctgata ataattgatg tatttcgttt gatttacttc tgtaatattc
     2041 agacattatt taattatcta catatttatt tatttttcaa caaaataatc atactattat
     2101 gtaataattt gtaaaaacga tgcaatatta tggcatctat atgatttatg tagataattc
     2161 aatatttaat gagcatctca tcattggaga tttctcgaca acagtcagcc ttttacggtg
     2221 tcaatctgat gaaatgccga atggatcgct catgcacggc ggtattcaat cggtaaccca
     2281 tgtgtttgcc atgggcctct cggacacact cgacaacctc acctcctgcc tgtgtgatct
     2341 tctgtttggc attttccacg tagaggctca ttttgatgcc ccacgactca tcttcatcgc
     2401 cactgtagag gaaaaaccgc cctcccacaa agggagcctt acctagacgg ccgctcacaa
     2461 agttttttaa aaaatcatcg gggtgggggt tttcgcccgg tgccaccacc agatccgtgg
     2521 caatccctcc tgaatgggcg atgaagagat caaagaattt tctgtgggtc
//
LOCUS       DATDUN010000215         2073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_175748, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000215 DATDUN010000000
VERSION     DATDUN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175748"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1739)
                     /locus_tag="VJC18_02875"
     CDS             complement(18..1739)
                     /locus_tag="VJC18_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLD44355.1"
                     /translation="MPQSLIKDILIIFACSIPVVLLLKRIHLPLVVGFLFTGALIGSH
                     GLGLITNAQSVDTLAELGLALLLFSVGLEFSLDSFAFIRKRAMTVALAQIILTCGAGW
                     LMGHWLGWPLGRSLYFGCVISLSSSAIVLSIFYEKKMGDSVPGRLTTTILIVQDLAFI
                     PMIVLLPLLGPDTPNDSLLLMTLANRGVAILVLIAIVFFGRLIINQVMRHIATLGQRQ
                     MFVIAVLILGLGISWLTASMGLSFALGAFVGGLLIGFTSYKHQALAEVAPFRHCFNSL
                     FFVSIGMLLNIQFVWDNFSIILLVLLLIPLTKLFITVGISTLVKIPLRVGIVTGLALA
                     QIGEFSFLLVHNGFRAGVIGPYLHDLIIAVAVFNMMLTPFLILNAHKWAAHINQLLLT
                     TKRFTKFACDDKQTISPIRQKLVDHVIICGFGPLGETLSHLFDKHNTPYVVLELNPKT
                     IQRIRKKNVIAFYGDGTSEEILYESRIENAKLIAITVPDFLDNIAIIKQARSLNPDLQ
                     IITRSKYRSDVDKLYAAGANVVISEELEGGIEMARYGLKMVGIPVNEVDQLMTHIREY
                     GSADFFN"
     gene            complement(1756..>2073)
                     /locus_tag="VJC18_02880"
     CDS             complement(1756..>2073)
                     /locus_tag="VJC18_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HLD44356.1"
                     /translation="RGHQIVVYLGDGIGLPHARVEGLKKPFLMFLRLKKGITCESSHD
                     KARMLFVLLTPAGQPRTHQKILAALATLLQESEYVKDRLLTARTPTQIMDVIRTGEQA
                     SLD"
BASE COUNT          539 a          513 c          573 g          448 t
ORIGIN      
        1 tgacaacgac cagcctctca attaaaaaaa tccgcgctcc cgtattcgcg gatgtgcgtc
       61 atgagctggt ccacttcgtt tacggggatg ccgaccattt ttaatccgta ccgggccatt
      121 tcgatcccac cctccaactc ctcactgatc accacattgg cccccgcggc gtagagcttg
      181 tccacatcac tgcggtattt ggagcgggtg atgatttgca ggtctggatt taaactacgt
      241 gcctgcttga tgatggcgat gttgtccaga aaatcaggga cagtgatggc gatgagtttg
      301 gcgttttcaa tccggctctc gtaaaggatt tcctcggagg tgccgtctcc ataaaaagcg
      361 atgacatttt ttttacggat gcgctggatg gtctttggat tgagttccaa caccacgtaa
      421 ggggtgttgt gtttgtcgaa caagtgcgag agagtttcgc ccaagggacc gaagccgcag
      481 atgatcacgt gatcaaccaa tttttgtctg atgggggaga ttgtttgttt atcgtcacag
      541 gcgaatttcg taaaacgttt ggtggtcaat agtagctgat tgatgtgggc ggcccatttg
      601 tgggcgttga ggatgagaaa gggcgtgagc atcatgttga acacggcgac cgcaatgatg
      661 aggtcatgca gataggggcc aatcaccccc gcacggaacc cgttatggac aagcaagaat
      721 gaaaattcac caatctgcgc caacgccaaa ccggtcacaa tccccacccg tagtggaatc
      781 ttgaccaacg tgctaatgcc cacagtgatg aaaagtttcg tgaggggaat cagcaagagc
      841 accaacagga tgatggaaaa attgtcccac acaaattgga tattaagcaa cattccgatc
      901 gacacaaaaa agaggctgtt gaaacagtgt ctgaacgggg cgacctcggc caaagcctgg
      961 tgtttataac ttgtgaaccc aatgagcaga ccgcccacaa aggcacccaa agcaaacgac
     1021 aggcccatgc tggctgtgag ccaactgatg ccgagtccca aaatcagaac ggcgatcacg
     1081 aacatttgtc tttggcccag cgtggcgata tggcgcatga cctgattgat gatgagcctg
     1141 ccaaagaata cgatcgcgat caaaaccagt atggccaccc cgcggttggc taacgtcatg
     1201 aggagcaagg agtcattggg tgtgtccgga ccaagcaggg gaagcagaac gatcatgggg
     1261 atgaaggcca ggtcctgcac aatcaggatg gtggtggtga gtctgccggg aacggagtca
     1321 cccattttct tttcgtaaaa aatgctgagc acaatggcgg aactcgacag cgaaatgaca
     1381 caaccaaagt aaagagaacg tcccaaaggc cacccgagcc agtgtcccat caaccaacct
     1441 gctccgcatg tgaggatgat ttgtgccaaa gcaacagtca tggcgcgctt gcggatgaag
     1501 gcgaagctgt cgagtgaaaa ttccagaccc acggagaaca gcaagagggc caaacccaat
     1561 tcggccaatg tgtctacgga ttgcgcgttc gtgatgagcc ccagcccgtg tgaaccgatg
     1621 agggctcccg tgaacagaaa accgacgacg agcggcaggt ggatgcgttt gaggagcaga
     1681 accactggga tggaacaggc gaagatgatg aggatgtctt tgatcaaaga ctggggcata
     1741 ggcaaagctc tgttgtcaat ccagtgacgc ctgttctccc gtgcggatca cgtccatgat
     1801 ctgggtcggg gtcctggccg tcaacaggcg gtctttgaca tactcgcttt cttgcaacag
     1861 tgtggccaat gcggccaata ttttttgatg agttcggggt tgtcctgccg gtgtgagcaa
     1921 aacaaacaac atgcgggcct tatcgtgcga actctcgcaa gtgatgcctt ttttgagtcg
     1981 cagaaacatg aggaacggtt ttttcaagcc ttccacacgc gcgtgcggca gaccgatccc
     2041 gtcacccaga tacaccacaa tctggtgccc ccg
//
LOCUS       DATDUN010000216         1043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_466496, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000216 DATDUN010000000
VERSION     DATDUN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466496"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..>1043)
                     /locus_tag="VJC18_02885"
     CDS             complement(108..>1043)
                     /locus_tag="VJC18_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HLD44357.1"
                     /translation="QQAKPSKRGKQIDPAKLKKQQIKAQNQAILKAVQNIMCKTKTTK
                     LFIYIDALDESYLPEKFPPHVQVILVTKNHEYNYSGPATIKGVLPIPKLKLGRMGLIK
                     IAVILGLSAKLVDTDDRIVFLLGKTGNEMLDTFMCFQIDEESELLTGHGLSDIPKTIV
                     PAVFESTLSLAIELGNQGKEGKPVGTIFVLGDEDRVMQLSRQMIINPFKGYEPEERNI
                     LNPTLKETVRELSSVDGAIVIAGNGEIISAGRYLGASTDNAEIPRGLGSRHLAAAGIT
                     ALTNAIAIVISESSGDVRIFKNGKIIMAIEKPIHK"
BASE COUNT          232 a          238 c          257 g          316 t
ORIGIN      
        1 ttccttttgg gagatttgac ctgatttcaa ataccgttct ctggtcctca catcgaaata
       61 tttgctatcg gcgtttttag tcatatgttt gctcttacgt tagaatgtta tttgtgtatg
      121 ggtttttcaa tagccatgat gattttgccg tttttaaaga tgcgaacatc accgctggat
      181 tcggagataa caatggcaat ggcgtttgtc aatgcagtga tgcctgcggc cgcaaggtgg
      241 cgtgaaccaa gccctcgcgg gatctcggcg ttatctgtgg aagcccccag ataacggcca
      301 gccgatatga tctcaccgtt tcccgcgatg acgatagcgc catccaccga ggagagttcc
      361 ctcactgttt ccttgagagt ggggttgagg atgtttctct cttctggctc gtaaccttta
      421 aaagggttga tgatcatctg acgcgataac tgcatgacgc gatcttcatc gcccaacacg
      481 aaaattgttc ccactggttt gccttctttg ccttggttgc ccagttcgat ggcgagactt
      541 aaggtgctct caaaaacagc aggaacaatg gttttgggga tatctgacaa cccgtgacct
      601 gtcagcagtt ctgattcctc gtcgatctga aaacacatga acgtgtccaa catttcgttg
      661 cctgtctttc caagcaaaaa gacgatccta tcatctgtgt cgacaagttt ggcagacaaa
      721 cccagaatga cagcgatttt gatcaacccc atcctgccca atttgagttt ggggatgggc
      781 agaacgccct tgatggttgc gggtcctgaa taattgtatt catgattttt ggtgaccaga
      841 atcacttgaa cgtgcggggg aaacttttcg ggcaggtagg actcatcaag ggcatcaatg
      901 taaatgaaga gcttggtcgt cttggtcttg cacatgatgt tctgcacggc cttcaagata
      961 gcctgattct gtgccttgat ctgctgtttt ttgagtttgg caggatcaat ttgtttgcct
     1021 cttttcgacg gtttggcctg ctg
//
LOCUS       DATDUN010000217         1123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_508230, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000217 DATDUN010000000
VERSION     DATDUN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508230"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..>1123)
                     /locus_tag="VJC18_02890"
     CDS             complement(263..>1123)
                     /locus_tag="VJC18_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44358.1"
                     /translation="PATTTPVPTTPPDTNTLLQLLPANWQDALVGEKAQLFARVGIDL
                     GIMAAITTGTWFATGLVKKVASHLTQRSDLSDREKYRVEAAIATARMLLVGGIFTASS
                     VVVGDSAMLATVNLLVGAAVAGFFLQETITSLYAYAILSNKQQYPLGSFIKVGGMRGF
                     VLARNLTHTTLIDFVEKAKVKPGEANPDERLDFEDDPTVSIPINVLHLLDGKQPIISV
                     FPNNSLLGCVEVVPQRHLNQKQLDHFLSLLSPDRKTPKASSTVRAQPAKLRPHEQTRV
                     VLIIDPNHPW"
BASE COUNT          300 a          262 c          293 g          268 t
ORIGIN      
        1 aagatcattg aactcgaatt ccctcaactc tccgtgttgt cgtatcagga actggccgag
       61 aatctgcgca tacagcctat cgcgcgcgtg aacctgccac ggctggcggc gaagaaataa
      121 tgtcccgcag agcgggatca gtcctacggc tgaaaacaat gaaacaatca agcaatcaag
      181 caatcaaaca atcaagcaat gaaataatca aaaaatgatt atcgattact tcattgattc
      241 attgattcat tgattcattt cctcaccacg gatgattggg atcaatgatg aggacgacgc
      301 gtgtctgttc atggggacgt agttttgcgg gctgagccct gacggtactg gatgctttgg
      361 gggtcttgcg gtcgggagac aggagggata aaaaatggtc caattgcttc tggttcaaat
      421 gacgttgggg tacgacttcg acgcacccca acagagaatt gttggggaac actgaaatga
      481 tgggttgttt gccatcgagc agatgcaaaa cattgatagg aatactcacc gtagggtcat
      541 cttcaaaatc aagacgttcg tctggattag cctctcctgg cttcaccttg gccttttcta
      601 caaagtcaat caaggtcgtg tgtgtgaggt tccgtgccaa cacaaaacca cgcatgcctc
      661 ccaccttgat gaaagaaccc aaggggtact gttgtttatt ggataaaatg gcgtaggcgt
      721 aaagggacgt gatggtttcc tgcagaaaaa agcctgcgac agccgcgccg accaaaaggt
      781 tgacggttgc cagcatggcc gagtcaccta cgacgactga actggcagta aagatgcccc
      841 ccacgagcaa cattctggct gtggcaatgg cggcctcaac acgatatttt tcacgatcac
      901 tcaaatcaga tctttgtgtc agatgagagg ccactttttt aaccaaacct gtggcgaacc
      961 aagtgcctgt tgtgatggcg gccatgattc ccaaatcaat tcccaccctg gcaaacaact
     1021 gggctttttc tcccacaagc gcgtcctgcc agttagcggg caataattgt aaaagagtgt
     1081 tcgtgtcggg aggggttgtg ggcacagggg tggttgtggc agg
//
LOCUS       DATDUN010000218         1532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_51675, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000218 DATDUN010000000
VERSION     DATDUN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51675"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1532
                     /locus_tag="VJC18_02895"
     CDS             <1..>1532
                     /locus_tag="VJC18_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HLD44359.1"
                     /translation="LDPKPLRDIKLLKRLKQSRYWVQQACQDLSEANRLEQSLPPTAE
                     WLLDNEYILEGNARDVLLNLPRGHYQKLPALESGPFKGLPRIYGLAREFVSGTDFRLD
                     EDNIIKYLEAYQNERALSIAELWTTPQMLRIALIEGIRHLSERALTELREEEMADFWA
                     HRLIMANRRDTKDLFSILAELTENQACPSLHFGAQLIDCLYDEGAVLTPVQNWLENTF
                     NKTFAELSLREKNHEMEDRVSIGNAFTSLRQLALIDWKVMFEKLSVVEKLLRTDPAGI
                     YPKMDFATRDRYRRCVEELHRGSHLTEEQVAQKAIDQARLAQSNRTADGRLAHLGAHL
                     IGEKRNHLAMLIGCRETLRFRLLHGIYAHHLKVYFLGFGFLFSLFLTLILFWGLGELT
                     CLAQCCLVFLLLIPVSQLSLDVMHYFITRFLPPRILPKMDYKISGIPDDCQTLVVVPM
                     MLSNLATVQAEADKLEVRYLANQQNNLLFGLLSDDTDANSPQTAEDATLLQAAVQAIE
                     RL"
BASE COUNT          374 a          383 c          380 g          395 t
ORIGIN      
        1 cgcttgatcc aaaaccttta cgcgacatca agttactcaa acgtctcaaa caaagtcggt
       61 actgggttca gcaagcctgc caggatcttt ccgaggccaa tcgattggaa cagagtttgc
      121 cccccacagc cgaatggctt ctcgataacg aatacatttt ggaaggcaat gcccgtgacg
      181 tgttactcaa cctgccgcgt ggtcattatc aaaaattgcc agcgcttgag agcggaccct
      241 tcaagggttt gccccgtatt tatggtttgg ccagggagtt tgtgtctggc actgattttc
      301 gtttggatga agataacatc ataaaatact tggaggctta tcaaaacgaa agggccttgt
      361 cgattgctga actctggaca actccccaga tgttgcgcat agcgcttatc gaaggcatac
      421 gacacctttc agaaagggcc ctcacggagt tgcgcgaaga agaaatggct gatttttggg
      481 cacaccggct catcatggcc aatcgacgag ataccaaaga cttgttttcc atactggcag
      541 aacttaccga aaaccaggct tgtcccagcc ttcattttgg ggcgcaactc atcgattgtc
      601 tctacgatga gggggcggtg ctcacgcctg tgcaaaactg gctggaaaat acgttcaaca
      661 aaacctttgc cgaactcagc ctgcgcgaaa aaaatcatga aatggaggac agggtatcca
      721 tcgggaacgc ctttaccagt ttgcgtcagc tggcgttgat tgattggaaa gtcatgttcg
      781 aaaagttgag cgtcgttgaa aaactgttac gtacggaccc tgccggcatt tatccgaaga
      841 tggattttgc cacacgcgac cgttatcgtc gctgtgttga ggaacttcac cgtggttctc
      901 atttgacaga agagcaggtg gcccagaaag ccatcgatca agcccgtctg gcgcaatcaa
      961 acagaacagc tgatggccga cttgctcacc tgggggctca cctgatcgga gagaaaagaa
     1021 atcatttggc aatgctgatc ggatgcaggg aaacacttcg gttccggttg cttcacggga
     1081 tatacgcgca tcacttgaaa gtctatttcc tgggttttgg ttttttgttt tctctttttc
     1141 tcacactgat ccttttttgg gggctggggg aattgacctg tctggcccag tgctgcctgg
     1201 tttttctttt gctcatccct gtcagtcaac tctctctgga tgtgatgcat tacttcatca
     1261 cgcgtttttt gccgccacgc attttgccaa aaatggatta caaaatctct ggcatccccg
     1321 atgattgtca gacgctggtg gttgttccca tgatgctgtc gaatttggca accgttcagg
     1381 cagaggcaga caaactggaa gtgcgttatc tggccaatca gcaaaacaac ctcctgttcg
     1441 gactcctgtc ggacgatacc gatgccaatt cgccgcaaac cgcagaagat gccacgcttc
     1501 tccaggcagc ggttcaggcc atcgaaagac tc
//
LOCUS       DATDUN010000219         1439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_260238, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000219 DATDUN010000000
VERSION     DATDUN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260238"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VJC18_02900"
     CDS             <1..410
                     /locus_tag="VJC18_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HLD44360.1"
                     /translation="RGEKTFNATDVDARRVVETLAMQAVIEHERLQGYEPRDVSKQNL
                     GWDIESMTNDGTLRLIEVKGRHHEATTVTVTRNEIMACLNKPELFIVALVIVKDGVAG
                     RPKYIRHFIKQEPDWAVASINYDLRDLVKAMES"
     gene            414..1091
                     /locus_tag="VJC18_02905"
     CDS             414..1091
                     /locus_tag="VJC18_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HLD44361.1"
                     /translation="MSDMLETIQSHCNECGHKTNHRVVFCDTVEGSEGDIDDQEHVQW
                     CTKWRLLKCLGCDDVSLEKRFWFSENCADGWDDPEYYPPRVARSKPRWFERLPDEYKG
                     LLNEIYKALQADGRRLAMMGARAIIDTFIQKKVGDKGNFEKGMEALRTSGFISTKNEE
                     IIKAAIDAGNASAHRAYNPPTDQIDAVMDIIENLIQHDILEEFSVGLKNTTPMRQINK
                     NGILNGN"
     gene            1081..>1439
                     /locus_tag="VJC18_02910"
     CDS             1081..>1439
                     /locus_tag="VJC18_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HLD44362.1"
                     /translation="MEIKTPKKLIEVALPLDAINAASAREKSIRHGHPSNLHRWWARR
                     PLATARAVIFSQMVNDPGWNNKLKRGLKKKEAAAERERLFEIIKKLVLWENTNNEAVL
                     NEDRAEIWKSWRETCEL"
BASE COUNT          450 a          267 c          373 g          349 t
ORIGIN      
        1 gaaggggtga aaaaacattt aacgctacag atgttgatgc gcgtcgtgtg gtcgaaactc
       61 tggccatgca agctgtgata gaacacgaac gcttgcaggg gtacgaaccg cgcgacgtgt
      121 ccaaacaaaa tctgggctgg gatattgagt caatgaccaa cgatggcacc ttgcgtctga
      181 tcgaagtcaa gggtcgtcat cacgaagcta ctacggtgac tgtgacacgc aacgaaatca
      241 tggcgtgttt gaataaacca gaattattca ttgtggcact ggttatcgta aaggatggtg
      301 ttgcagggcg tcccaaatat attcggcatt ttattaaaca ggagcctgat tgggcggtgg
      361 cgagtattaa ttatgatttg agggatttgg tgaaggcgat ggagagttaa cttatgtctg
      421 atatgctcga aacaatacaa agtcactgta atgagtgcgg acacaaaaca aaccaccgag
      481 tggtcttttg tgatacggtg gagggaagtg agggtgatat tgatgaccag gaacatgttc
      541 aatggtgcac aaaatggcga cttctcaaat gtcttggttg tgatgacgtg tctctcgaaa
      601 aaagattttg gttttcagag aactgtgcag atggatggga tgatcctgag tattatccac
      661 cgcgtgtagc aagatcgaaa ccgagatggt ttgagcgatt gccagatgaa tataaagggt
      721 tgcttaatga aatttataaa gcacttcagg ctgatggtcg taggttagca atgatggggg
      781 ctcgtgcaat tattgatact tttattcaaa agaaagttgg ggacaaaggt aattttgaaa
      841 agggcatgga agcacttcga acgtcaggat ttatcagcac aaaaaatgaa gaaattatta
      901 aagcggcaat agatgcagga aatgccagtg ctcatcgtgc ttataacccc ccaacagatc
      961 aaatcgatgc cgtaatggac attatagaga acctcattca acacgatatt ctggaagagt
     1021 tttccgttgg actgaagaat acgacaccaa tgaggcagat aaacaagaat ggaattttga
     1081 atggaaatta agaccccaaa aaaactcatc gaagttgccc tcccactcga tgcaatcaat
     1141 gctgcttccg cgcgtgaaaa atcaattcgt cacggccatc catcaaatct ccatcgttgg
     1201 tgggccagga gacctttggc cactgcacgg gcagtcatat tttcacagat ggtcaatgat
     1261 ccgggttgga acaacaaact caaacggggg cttaagaaaa aagaggctgc tgctgaacgc
     1321 gaacggcttt ttgaaatcat caaaaaactg gtgttgtggg aaaacaccaa taacgaagca
     1381 gtgctcaacg aagaccgtgc tgagatttgg aaaagctggc gtgaaacgtg tgaactcaa
//
LOCUS       DATDUN010000220         1176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_175887, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000220 DATDUN010000000
VERSION     DATDUN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175887"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..>1176)
                     /locus_tag="VJC18_02915"
     CDS             complement(352..>1176)
                     /locus_tag="VJC18_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD44363.1"
                     /translation="KTTINIFGYRDYRAYLKDWFARAKETQRGFSHRYFCKQAGFNTS
                     NFLMLVMSGKRNLTDHSVTRVVKGLGLGRQEEEFFRNLVYFNQSRTNDEQSLYFERLL
                     QCKKFTEATPIEAKQYEYYSAWYHPVIRELVCSADFDGTPEWLAKRLYPTVSPQLCKK
                     SMEILLELGFIKKKGRGYEQSSAIVSTGAELTSVIVHNYHKSILELSKAVMDKLSTKD
                     RDVSTLTLGVKKNRIAQIRARIKDFRKEILGMVSADTEPDEVVQLNMQFYPVTRKG"
BASE COUNT          273 a          344 c          217 g          342 t
ORIGIN      
        1 acagagctgg cgttgatttc gggaatcaga acctctgccg cgctcccaaa atggctgacc
       61 agattggcat agacccctcc tgtatcagta gaccctgtct cggtccctcc caacccgcct
      121 ccgcaaccag ccaaaatcac caatgtcgcc atcaccaaac tgtaattaat ttttttcata
      181 ttatctcctc tcgtttcttc attttacatt ttacattttt cattctgcat tttgcactct
      241 gcattttgca ctctgcattt tgctttcccc tcacccatta cccattaccc tttgaccctt
      301 cctccctgcg actctgcgct tccgcgaccc ttctccccct cccatcaccc atcacccctt
      361 cctcgtcacc ggataaaact gcatattcaa ctgcaccact tcgtccggtt cggtatcggc
      421 cgataccatt cccagaatct ctttgcgaaa atcttttatt ctggccctga tttgcgcgat
      481 cctgtttttt ttgacaccca aagtcagggt gctcacatca cggtccttgg tggacagttt
      541 gtccatcacc gcctttgaga gctcaagaat gcttttgtga taattatgca caatcaccga
      601 ggtcaactcc gcacccgtag atacaatggc actggactgc tcataaccac gccccttttt
      661 tttgatgaac cccaactcca acaggatttc catggatttt ttgcaaagct gtggcgacac
      721 agtaggatac aatcgtttgg ccagccattc cggggttcca tcaaagtcgg cagagcaaac
      781 cagctcacga atgacgggat gataccaggc agaataatat tcgtattgtt tggcctcaat
      841 gggagtcgcc tcggtgaact ttttgcactg caagagtctt tcgaaataga gactttgttc
      901 gtcgttggtt ctggactgat tgaaataaac gagatttctg aaaaattctt cttcttgtct
      961 gcccagcccc aaccccttta ccaccctggt cacactgtga tcggtcaaat tgcgcttgcc
     1021 gctcatcacc agcatcaaga aattgctagt attaaaaccg gcctgtttgc aaaaataccg
     1081 gtgagaaaaa cccctttgcg tctctttggc tcgcgcaaac caatctttaa ggtaggccct
     1141 gtaatcccga tagccaaaaa tgttgatggt ggtttt
//
LOCUS       DATDUN010000221         1025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_592555, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000221 DATDUN010000000
VERSION     DATDUN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592555"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..726)
                     /locus_tag="VJC18_02920"
     CDS             complement(175..726)
                     /locus_tag="VJC18_02920"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HLD44364.1"
                     /translation="MRLIDCDTFFFPFSTPHFSGHSMYEKIIRILASGLGLGYLPLAP
                     GTFGTVLGSVIFYLVRMQSHAWLINLTLVVVLFSLVIAHLAEKEFGEKDCQKIVIDEV
                     AGVLVCYLFVPYSLFNLVMGFILFRLFDIAKIFPARQAQDHLDGGLAVVGDDLVAGVQ
                     AGLLLLFFPQILHWVEVGLTYLA"
     gene            complement(736..>1025)
                     /locus_tag="VJC18_02925"
     CDS             complement(736..>1025)
                     /locus_tag="VJC18_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44365.1"
                     /translation="IFIHAGLLMCFTPHNEQTRASVDSTLLEIAQARQYYTVSSNTAT
                     TGQGMHQGHDERTKTVVKKRATQTKKQTRDDLSVEGSGSQPDLYISHSVYT"
BASE COUNT          292 a          263 c          236 g          234 t
ORIGIN      
        1 atccctggca agcgtgttag atgaatctgc cgcggtcaaa ttggcctacg tgtgtgaaaa
       61 actgcgaacc ggcatcgaaa ttcgtcaaga actcgaacag tttttcatca gaaataaatt
      121 tgatcatttc ctgcacgaaa ccagggccgc gctgacggat tattagaagt gcccttaagc
      181 caaataggtt aacccaacct cgacccaatg cagaatttgg ggaaagaaca acaacaaaag
      241 ccctgcctgc acccccgcca ccagatcatc accaacaacg gccaacccgc catccaaatg
      301 gtcttgcgct tgtctcgcgg gaaaaatttt ggcaatgtcg aacaagcgaa acaaaatgaa
      361 tcccatcacc agattgaaaa gtgaatacgg gacaaacagg taacatacca gcacgccagc
      421 cacctcatcg atcacgatct tttggcaatc cttttctcca aactcttttt ctgccagatg
      481 agcgatgact aacgaaaaca agacaaccac caaggtcaga ttgataagcc acgcgtgcga
      541 ttgcatacgg accagataaa aaatcacact ccccagaacg gtcccgaagg tgccgggcgc
      601 caggggaagg taaccgagac cgaggcccga agccaaaatc cgaatgattt tttcgtacat
      661 gctgtggcca ctaaaatggg gggtggaaaa aggaaagaaa aaagtatcgc aatcgatgag
      721 ccgcacgcca tccgctcacg tatacacgct gtggcttatg tagagatcag gttgagaacc
      781 tgatccctcg acagaaagat catcacgagt ttgttttttg gtttgggtcg ctctcttttt
      841 gaccaccgtt tttgtgcgct catcgtgtcc ctgatgcatg ccctgtcccg ttgttgctgt
      901 gttactagac acagtgtaat attgtctggc ttgggcgatt tctaacaacg tgctgtccac
      961 actggcccga gtttgttcgt tgtgcggtgt aaaacacatg agcagtcccg cgtggatgaa
     1021 tattt
//
LOCUS       DATDUN010000222         1097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_260352, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000222 DATDUN010000000
VERSION     DATDUN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260352"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VJC18_02930"
     CDS             <1..509
                     /locus_tag="VJC18_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44366.1"
                     /translation="PGRFIMTEPLSLPTSKLPLSTDDAQAKSVEPACGDGKTPLTITI
                     EDSRGKARYYEIQNSEIVLRGSQQPLEAIPVVPSFQTATAVLPRGTTRVLVTRLFADE
                     RNDQIETQSATINSGSPFVFSFSGLAVGDSVVFYLCPGNADTVCVGGAGNCYLTSIKF
                     MVVSLAQE"
     gene            complement(563..>1097)
                     /locus_tag="VJC18_02935"
     CDS             complement(563..>1097)
                     /locus_tag="VJC18_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44367.1"
                     /translation="MLSRAKGKNQDSRDAFFCETFEQFTGRGVKKNGQYDVVSALNSL
                     YVLTDKAQIVSVLNGIREHLVDGGQFMASILHEIDPENMLRAQFGLPRTEPKNDMASA
                     LKLGFSHDPLLTLRLFLPGVITKILGASSNTLLDKATWTRLIESAGLVIDDAAGYYGG
                     ANYGEPLPGTFFLAHKA"
BASE COUNT          294 a          283 c          248 g          272 t
ORIGIN      
        1 tcccaggacg atttattatg acagaaccac tttccctccc cacctcaaag ctacctctat
       61 cgacagatga tgcacaggcc aagtctgtcg aaccagcctg cggagatggc aaaacccctc
      121 tcaccatcac gattgaggac agtagaggca aagcgcgcta ttacgaaata caaaactcag
      181 aaattgtttt gagaggctcc caacaacctt tagaggcaat tcctgtagtc ccttccttcc
      241 agacagccac cgccgtgctc cccagaggca caacccgagt tttggtgacg agattgtttg
      301 cagatgaacg caacgatcaa atagaaactc aatccgcaac aattaattca ggctcgccat
      361 ttgttttttc gttcagtggt ttggctgtgg gagattctgt tgttttttat ttgtgtccgg
      421 gaaatgctga tacagtttgt gttggcgggg cgggcaattg ttatctgacc agcataaaat
      481 ttatggtggt ttctttggca caggaatgaa atcaaacaac aagagttaac ctgataaaca
      541 tgactgacgg cacagccacg ttctacgcct tatgcgccag gaaaaacgta cccggcagtg
      601 gttctccgta attggcgccc ccgtaatatc cggcggcgtc atcaatcacc agaccagccg
      661 actcaatcag ccgcgtccag gtggctttat ccaatagcgt gttgctggaa gcacccaata
      721 ttttcgtgat cacaccgggc agaaaaagac gcaatgtcaa aagcggatcg tggctaaacc
      781 ccaactttag agccgaagcc atatcgtttt tgggttccgt tctgggcaaa ccaaattggg
      841 ctctgagcat attttcggga tcgatttcgt gaagaataga ggccatgaac tgtcccccat
      901 caacaaggtg ctcccgtatc ccattcaaga cggaaacaat ttgcgcctta tcggttaaaa
      961 cataaagtga gttcagcgca gaaacgacgt cgtactgtcc gttctttttc accccacgcc
     1021 ctgtaaattg ctcaaaggtt tcacagaaga aagcgtcgcg agaatcctga ttcttgcctt
     1081 tagcgcggct tagcatc
//
LOCUS       DATDUN010000223         1465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_51811, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000223 DATDUN010000000
VERSION     DATDUN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51811"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1465)
                     /locus_tag="VJC18_02940"
     CDS             complement(<1..>1465)
                     /locus_tag="VJC18_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44368.1"
                     /translation="HARISFEQGRYVLRTDHPLFVLRDGDEIAVGRHQQGLRNSHLIL
                     RNGDVIVFKKYTLTFKVILPFSLLSDVTRDWDTEMRAWSYVATLSDVIRLFSEAQETV
                     RGRANVIVEVPVYHDIPPEALDEIDAHCRRALRTYPRLAGYQLVRARNLGGCKPHTTD
                     MFYCDPPVFEIDIARTISVSDAPRTIAHQPDATCMVTPSRLRDIVNATEEYQGHRLGD
                     VVTVLRTRQGLRGPVKVPERGWTITAFDTLRQRVTVRRAMTADEVTQHAANGFIDGVK
                     RILFGHVERPNELELTVPLEYIATDQQIFDAVNGEQTAFWHLSRRVALLRDHAKMTYA
                     EFQALFGGEFHQQNVGNCYLISVLKALTSDPENFEALMRLSIRPVKEGWEVTVPLGLL
                     NGRKIIITRENLRPCHNPHRGEPDYSRPDEIDSRKSLEPVSGPDGYRILEAVYCKLLK
                     GERGATYVPVDRSVIDQGGFSREAIVTLLGDGIIAERL"
BASE COUNT          361 a          408 c          353 g          343 t
ORIGIN      
        1 cagtctttca gcgataatgc catcacccag cagggtcaca atcgcctcac gactgaaacc
       61 accctgatca atcacagaac gatcaactgg cacataagtg gcccccctct caccttttag
      121 aagtttacaa tagacggcct ccaatattct gtagccatcg gggcctgata caggctccaa
      181 tgatttccta gaatcaatct catcgggccg cgaataatca ggctcacccc ggtgtggatt
      241 gtggcaaggc cttagatttt cgcgcgtgat aatgatcttc cggccgttga gaagtcccag
      301 cggtacggtc acctcccagc cttctttaac gggcctgatg gacagccgca tgagtgcctc
      361 aaaattttcg ggatcgcttg tgagtgcttt aaggacagat atcaaataac agttgcccac
      421 gttttgctga tgaaattcac caccaaaaag cgcctggaac tctgcgtatg tcatcttggc
      481 atgatcacgt agtaatgcga cacgtcggct caggtgccaa aatgcggtct gctccccatt
      541 aactgcatcg aaaatttgct gatcagtcgc tatgtattcc aggggaacag tgagttccag
      601 ttcgttgggc cgttccacat gaccaaataa tattcttttg actccgtcga taaaaccatt
      661 ggccgcgtgc tgtgtgactt catcggctgt catggcgcgt ctgacagtca cgcgttgccg
      721 cagtgtatca aaagcggtga tggtccaacc ccgttcaggt accttcacag gccctctcaa
      781 tccctgacgc gtacgcagga ctgtcacgac atctcccaag cggtgccctt gatattcttc
      841 ggttgcgttc acaatatccc tcaagcgtga gggggtgacc atgcacgtgg cgtctggctg
      901 atgggcgatg gtcctgggcg catcggacac gctgatcgtt ctcgcaatat caatttcaaa
      961 gacaggaggg tcacaataaa acatgtcggt cgtatgcggt ttgcacccgc ctagattgcg
     1021 cgcacggaca agctgatacc ccgcaagccg cggataagta cggagggcac gacgacaatg
     1081 ggcatcgatt tcgtccagag cctctggggg aatatcgtga taaacaggca cctcaacgat
     1141 cacattggcc cgcccacgca ctgtctcttg agcctccgaa aacaacctga tcacatcact
     1201 cagcgtggcc acatatgacc aggccctcat ctccgtatcc caatctcgcg tcacatcgct
     1261 caataatgaa aacgggagaa tgaccttgaa ggtcaaagtg tattttttaa aaacaatgac
     1321 gtcaccgttt cgcaatatga ggtgggaatt acggagacct tgctggtgtc tccccacggc
     1381 gatttcatcg ccgtccctca agacaaacaa gggatggtct gttcgcaaga cataccggcc
     1441 ctgttcaaaa gaaatccgtg cgtga
//
LOCUS       DATDUN010000224         3244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_301177, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000224 DATDUN010000000
VERSION     DATDUN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301177"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VJC18_02945"
     CDS             <1..263
                     /locus_tag="VJC18_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44369.1"
                     /translation="LVSPEQEGLEFLGPKFSAESQHGLSFFVMAQDEPAMIGTVNLRT
                     HPKLKQILDALPQNNAEGQVIPLFNNCTENLVNVEEVTEFKN"
     gene            337..1362
                     /gene="tdh"
                     /locus_tag="VJC18_02950"
     CDS             337..1362
                     /gene="tdh"
                     /locus_tag="VJC18_02950"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HLD44370.1"
                     /translation="MKALVKKYPQKGLWLEEIPTPQIEANEVLIKIKQTAICGTDVHI
                     YNWDEWSQKHVTVPMQVGHEFVGEIVEMGKEVKEHFMGERVSGEGHLVCGHCRNCKAG
                     RRHLCCHTRGVGVDHPGAYAEYLALPASNVFSVPADISDNEASIFDPLGNAVHTALSY
                     DLVGEDILITGAGPIGIMAGAIAKHAGARHVVITDINPYRLELAKKMGIKHCVNVKEK
                     SLKDEECALGMTEGFDVGFEMSGSPMALQDMLEAMANGGRVALLGILPPDTKISWDHV
                     IFKGLKIKGIYGREIFETWYKMCAMIQSGLNIRPICTHEYPVEKFEDGFKVMSSGNSG
                     KVILKWA"
     gene            1445..2686
                     /locus_tag="VJC18_02955"
     CDS             1445..2686
                     /locus_tag="VJC18_02955"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HLD44371.1"
                     /translation="MVYQTPQVFFKGVLNKIKSDGLYKEERYICSPQDGKIKARYPLD
                     APVKEIINLCSNNYLGLSSHPEVIKAAHAALDARGYGMSSVRFICGTQDIHRELELKM
                     SSFLGTQDTILFASCFDANAAVFEALLGKDDAIISDKLVHASLIDGIRLCKAQRFIYS
                     HSDMDELEKVLKSINALNKVIVTDGVFSMDGDLAKLDAICALAEKYEAMVLVDDSHAT
                     GFIGKTGRGTPEHFGVMDKVDIITTTFGKALGGATGGCVSAKKEVVDILRQKGRPYLF
                     SNALMPAVVQATMRVLELLSESTQRRDKLEQLTAFWRDSLIKAGFDIKPGTSPIVPIM
                     LYNAQLSQDISRDLWAEGLYAVGFFYPVVPKGQARIRTQMSASLEKADLEKALEVLIR
                     VGKKYDILGKSRSDVVARYGE"
     gene            complement(2700..>3244)
                     /locus_tag="VJC18_02960"
     CDS             complement(2700..>3244)
                     /locus_tag="VJC18_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HLD44372.1"
                     /translation="HDIDDGLKAGLLTMRALEKVSLWQSHFEHICQQHTNLTLTQQRR
                     FTVNAIINHLVTDLIENTSANIDDLGIASIDDVRKRGKELVSFSAPCRQDNAELKEFL
                     YRSLYTHPKVLNMQKRAKVVIAGLFNSLIKESKLIPGEFIAFYPQDEPQPRIVCDYVA
                     GMTDRFALGEFDRLFEEIHH"
BASE COUNT          791 a          727 c          911 g          815 t
ORIGIN      
        1 ggcttgtgtc accagagcag gaaggactgg agtttttggg gccaaaattt tcggcagaat
       61 cacagcatgg tttgtccttt tttgtcatgg cacaagatga acccgccatg ataggaacgg
      121 tcaatttgcg aacgcatccc aaactcaaac aaattcttga tgccctgcca caaaacaacg
      181 cggaggggca ggtgatcccc ttgtttaata attgtaccga gaacctggtc aatgttgagg
      241 aagtgactga attcaagaat tagaggtccc ctgcagacac gaaaaatcat tgttttcatt
      301 tgaaatcagc tatcagtccg attatgttcc ccagtcatga aagcactcgt caagaagtat
      361 ccccaaaaag gtttatggct cgaggaaatt cccaccccgc agattgaagc gaacgaggtt
      421 ctcatcaaaa tcaaacaaac agctatttgt ggtacggatg tgcacatcta caattgggat
      481 gagtggtcgc aaaaacacgt gacagtgccc atgcaggtag gacacgagtt tgtgggcgaa
      541 attgtggaaa tgggcaaaga ggtcaaggaa cattttatgg gtgagcgagt ctctggtgaa
      601 gggcatctgg tttgtggcca ttgccgtaac tgcaaggccg ggcgacgcca tctgtgttgt
      661 cacacgcgag gcgtgggcgt ggatcacccc ggggcgtacg cggaatatct ggctttgccg
      721 gccagcaatg tgttttctgt tcctgctgac atctccgaca acgaggcttc catttttgat
      781 cctttgggca acgcggtgca cacggcgctg tcgtacgatc tggtgggtga ggacattctc
      841 atcacagggg ctggtcctat tggcatcatg gcgggggcca ttgccaaaca tgcgggggcg
      901 cggcatgtgg tcatcaccga tatcaaccct tatcggttgg aactggccaa aaaaatgggc
      961 atcaagcact gcgtcaatgt caaagaaaaa tccctcaagg atgaggaatg cgcattgggc
     1021 atgacagagg gttttgatgt ggggtttgag atgtcggggt cacccatggc tttgcaggat
     1081 atgcttgagg ccatggctaa cggcgggaga gtggctcttt tggggatttt gccacccgat
     1141 accaaaatca gttgggatca tgtgattttc aaggggctca agatcaaggg catttatggc
     1201 agggaaattt ttgaaacctg gtacaagatg tgcgccatga ttcagagcgg gctcaacatc
     1261 cgtcccatct gtacccatga atatcctgtc gaaaaattcg aagatggttt taaggtgatg
     1321 tcatcgggca attcgggcaa agtgatactg aaatgggcgt aaggcgtaga gcattaggca
     1381 taaggcgtag ggcgtaaggc gtagggttgg tcgcgaccaa cccctacact caaagggggg
     1441 ataaatggtt tatcaaacac cgcaggtttt ttttaaggga gtgctcaata agatcaaaag
     1501 cgatggtttg tataaagagg agcgctacat ttgttctcct caggatggca agatcaaggc
     1561 ccggtatcct ttggacgcgc cggtcaagga gatcatcaat ctgtgttcca ataattatct
     1621 gggactttcc agtcacccgg aggtgatcaa ggcggcacac gcggcccttg acgcgcgtgg
     1681 ttatggcatg tcgtcggtgc ggttcatctg cggcacgcag gatattcatc gagaattgga
     1741 acttaaaatg agttcatttt tgggaactca ggacaccatt ttattcgcgt cctgttttga
     1801 tgccaacgcg gcggtgttcg aggccttgct cggtaaggac gatgccatta tttctgataa
     1861 acttgtgcat gcctcattga tcgatgggat cagactttgc aaggcacaac gcttcattta
     1921 ttctcattcc gacatggatg agctcgaaaa agtgctcaaa tcgattaacg cgctcaacaa
     1981 agtgatcgtg acggatggcg ttttttcgat ggatggtgat ttggccaagc ttgatgccat
     2041 ctgcgcattg gccgaaaaat acgaggcgat ggtgctggtg gatgattctc acgcgaccgg
     2101 ctttattggc aaaacaggac gtggcacgcc agagcatttt ggcgtgatgg ataaggtcga
     2161 catcatcacg accacgtttg gcaaggcttt gggcggggcg actggcgggt gtgtgtccgc
     2221 gaaaaaagaa gtggtggata ttttaagaca aaaggggcga ccctatttgt tcagcaacgc
     2281 cctcatgccg gcagtggtgc aggccacgat gagggttttg gaactgttgt ccgaatccac
     2341 gcaaagacgt gacaaactcg aacaactcac cgcgttctgg cgcgattcct tgattaaggc
     2401 tggtttcgac atcaaacccg ggacctcacc gattgtgccc atcatgttgt acaacgcgca
     2461 actctcccaa gacatcagtc gtgatttgtg ggccgagggt ctgtacgccg tgggtttctt
     2521 ttatcccgtg gttcccaagg ggcaggcgcg tatccgcacg cagatgtccg cctcgctcga
     2581 aaaggccgat ctggaaaaag ccctcgaagt gcttatccgt gtggggaaaa aatacgacat
     2641 cctcggaaaa tcccgttctg atgttgtggc ccggtatgga gagtaggcct tgctgtttgt
     2701 cagtgatgga tttcttcaaa taacctgtca aattcaccca gtgcgaagcg atcagtcatc
     2761 ccggcgacgt agtcgcagac gatgcggggc tgtggttcgt cttgaggata aaatgctatg
     2821 aactcaccag gaatgagttt cgattctttg atgagggaat tgaagagtcc tgcaatgacc
     2881 accttggccc tcttctgcat gttgagcacc ttggggtgcg tgtagaggga acgatacaga
     2941 aactccttga gttccgcgtt gtcctgccga catggcgctg aaaaagacac cagttccttg
     3001 ccccgctttc tcacatcatc tatcgatgcg atgccaaggt catcaatatt tgctgacgtg
     3061 ttttcgatca ggtctgtgac cagatgattg atgatggcgt tgacggtaaa gcgtcgttgt
     3121 tgtgtcagtg tcaggttggt gtgttgttgg cagatgtgtt cgaaatgact ttgccagagc
     3181 gaaacctttt caagcgccct catcgtgagc agacctgcct tgaggccatc atcaatgtcg
     3241 tgat
//
LOCUS       DATDUN010000225         2526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_95368, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000225 DATDUN010000000
VERSION     DATDUN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95368"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..326
                     /locus_tag="VJC18_02965"
     CDS             201..326
                     /locus_tag="VJC18_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44373.1"
                     /translation="MANLIAGVIARTPFGNWLFGQFAKIITATASYGGRSPRDRK"
     gene            complement(527..808)
                     /locus_tag="VJC18_02970"
     CDS             complement(527..808)
                     /locus_tag="VJC18_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HLD44374.1"
                     /translation="MKSNKQSLSETLLSVAQIDPSLPSLDLHGLSVHEAELEVDHFLS
                     QQLYRKERVVRIICGVGTGALADAIWEFVQKHPVVEMAKSKGSEIFVVL"
     gene            complement(905..1330)
                     /gene="tsaE"
                     /locus_tag="VJC18_02975"
     CDS             complement(905..1330)
                     /gene="tsaE"
                     /locus_tag="VJC18_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HLD44375.1"
                     /translation="MFTSKSEQQTKDIANDFAKTLKGGEVIELVGDLGTGKTVFVRGI
                     AQAFGSDARVKSPTFTIMNEYPVKAQQVKKVIHIDLYRFNDASQLSALELDEVMTADS
                     VVCIEWPDIFGQSPFHPTHRIKIDHVDEETREIEIESLS"
     gene            complement(1332..2396)
                     /gene="tsaD"
                     /locus_tag="VJC18_02980"
     CDS             complement(1332..2396)
                     /gene="tsaD"
                     /locus_tag="VJC18_02980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HLD44376.1"
                     /translation="MSVCFCCMRILGIESSCDETAIALVTGKGDWLKVENSTVLSQIQ
                     THQQYGGVVPEVAAREHLAAIFPMLERELPFDGQGIDAIAVTSGPGLAPALRVGVEVA
                     KALAWSWGAPLIPVCHMEGHVYASWLPRSKRLFPFFPQRVPKFPALSLIVSGGHTELV
                     MMHDHGKFERIGETLDDAAGEAFDKVAKMLGLQYPGGPNIARLAVGGNKTAFDFPRGM
                     IERDDFTFSFSGIKTSVLYTLRKNEDKLDDAKFKSDIAASFQEAVVDVLTQKSMRAIE
                     QYKPKSFILAGGVAANIELRNRIIKEGERRVTRVFVPPFSYSVDNAAMIAAAGYFRAR
                     NPKNFIEPVDLVADSNMDIV"
BASE COUNT          718 a          672 c          479 g          657 t
ORIGIN      
        1 gttttctggt gccacatcac ctacgatgag aagttggacg gggggacacg accaagatga
       61 cacttccgac ccctccttcg cccagcgagc tcgctgggcg tccgccaatt ctcatcacca
      121 acatcaagtt aatcgacgat cgtcgattaa ctcacatgtg atgagcatcg gtggacctac
      181 aacgccatgg aggaaaaatc atggcaaacc tcatcgcggg cgtaattgcc cgcaccccct
      241 tcggcaactg gcttttcggc cagtttgcca agatcatcac ggcgaccgcc agctacggtg
      301 gtcgctctcc ccgcgatcga aaatgatcgt gggcctgcca attctcatca ccaacatcaa
      361 gttaatcgac gatcgtcgat taactcacat gtgatgagca ttggcagata cgcaaaacaa
      421 cccacacacc tattattaca gtaaaaatga taataggtgt gtgggttttt tgttttgtat
      481 tttatgcatc cacatctttt taagatggta atacaaatat tatatcttat aacactacaa
      541 aaatctctga cccttttgat tttgccatct cgaccactgg atgtttttga acaaattccc
      601 aaattgcgtc agcgagtgcg cctgttccaa ctccacaaat tatcctcaca actcgttctt
      661 ttctataaag ttgttgcgat aaaaaatgat caacctcaag ctctgcctcg tgaacactta
      721 acccgtgcaa atccaagctc gggaggcttg gatctatttg ggcaacactt aaaagtgttt
      781 cgctaagact ttgcttgttt gatttcataa acacacgact ccctccgtcc ttcgccccac
      841 taatgatgga gctatggaac ggcaggctac ctcccccttc gaaaggggag aaattagaca
      901 aaaattaact aaggctttca atctcaatct ctctcgtctc ttcatcaaca tgatcgatct
      961 taatgcgatg agttggatga aatggtgact gaccaaaaat atctggccat tcgatacaaa
     1021 caactgagtc agctgtcatg acctcatcaa gctccagagc agaaagttgc gaagcatcat
     1081 taaaacgata tagatcaatg tgaattacct ttttaacttg ctgagcttta actggatact
     1141 cattcataat cgtaaaagtc ggacttttga ctctggcgtc agacccaaac gcctgagcaa
     1201 tcccccgcac aaaaacagtt ttgccagttc ccaaatcacc aaccaactca atcacttccc
     1261 cacccttaag cgtctttgca aaatcattgg caatatcttt tgtttgttgt tcagactttg
     1321 atgtgaacat atcaaacaat atccatatta gaatccgcca ccaaatccac cggctcgatg
     1381 aagttttttg gattccgagc gcggaagtaa ccagctgcgg caatcatagc cgcattgtct
     1441 actgagtaag aaaatggtgg tacaaaaact cttgttactc gtctctcccc ttctttgata
     1501 atcctatttc gaagttcaat gttcgccgcc actccgccag ccaaaataaa tgactttggt
     1561 ttgtactgct caatggctct cattgacttt tgagtaagca catcaaccac tgcttcctgg
     1621 aacgaagcgg caatgtcgct tttgaactta gcgtcatcaa gcttgtcttc attttttcgt
     1681 aaagtgtaaa gcactgaagt tttgattcca gaaaaactaa atgtaaaatc atcccgctca
     1741 atcatgcccc gcggaaaatc aaaggcagtc ttattcccac caactgccag gcgagcaata
     1801 tttggtccac ccggatattg cagtcctaac atcttggcaa ctttatcaaa cgcctcacca
     1861 gcggcgtcat caagtgtttc accaatccgc tcgaactttc catgatcatg catcatcacc
     1921 aactcagtgt gaccacctga cacgattaaa ctcaaggcag gaaattttgg aactctttgc
     1981 ggaaaaaatg gaaacaatct ttttgatcgt ggcaaccagc tggcgtaaac atgaccttcc
     2041 atgtggcaga ctggaataag tggtgctccc caactccaag ccagtgcttt ggcaacctcc
     2101 acgccaactc gcaaagccgg tgccaagcct gggcctgagg taacagcaat agcatcaata
     2161 ccttgaccgt caaacggaag ctcacgctct agcataggaa aaatcgctgc taagtgttca
     2221 cgagctgcca cttctggcac aaccccacca tattgctgat gagtttgaat ctgcgacaac
     2281 acagtcgaat tctcaacctt aagccaatca cccttgcctg ttaccaaggc tatggccgtt
     2341 tcgtcacaac tagattcaat cccaagtatt cgcatacaac aaaagcatac agacattgac
     2401 aattttgagc aaattgtcta tcctcacacc ttcttgcttt gcacttcgca tcgcagaaga
     2461 gttcatcaag gtgaaaggag cgaccatgga cgacaaccgc cacggtctga ccaacgccga
     2521 gcgagc
//
LOCUS       DATDUN010000226         1593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_467151, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000226 DATDUN010000000
VERSION     DATDUN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467151"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1454)
                     /gene="hemN"
                     /locus_tag="VJC18_02985"
     CDS             complement(78..1454)
                     /gene="hemN"
                     /locus_tag="VJC18_02985"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HLD44377.1"
                     /translation="MLSVIELLNKYDVAGPRYTSYPTLPVWANQVTAADYQTSLSQIR
                     RDDQLSLYVHIPFCENQCHFCACSRIATTDHSLSANYVHTVIKELELVAQNLQNTKAP
                     VTQIHFGGGSPNFLHPTELSLIMRAINKHFDVLQSAELAIEINPKTTSPAFCAVLGRE
                     GFNRVSLGVQDFSAQVQQLIHRHQTSEDTQNTLSQLKTHGVTHFNFDLVYGLPGQSLR
                     TFQTTLETVLSLDPDRLAIYSYAHLPWTQDKQRSFDERDLPSNKLKLKLFSEALQFLT
                     AHNYHQIGLDHFAKTNDELYQALKNKTIHRNFMGYSTRAQAHQIGLGATSISFVNGNY
                     FQNEKNVVDYQNLIAKQQLATTKGHLLSHDDTIRRDLINQIMCHQFVDITVFEKKHTI
                     HFADYFKNELLRLKEFAADGLVTLDNNILAVTRQGQITLRNIAMCFDAFLHQRDVNTI
                     KTKFSRTI"
BASE COUNT          365 a          325 c          460 g          443 t
ORIGIN      
        1 tatcccatca tgtatcccca gtctgaactc aatatcgccg gtgttgtgac cggggtcgtg
       61 cggaagtatg ggtagatcta aatcgttctc gaaaattttg ttttgatggt attgacatcc
      121 ctttggtgca ggaaggcatc gaaacacatg gcgatgttgc gtagcgtgat ttgtccctgc
      181 cgggtgaccg caagaatgtt gttgtcgaga gtcaccagtc catcggcggc aaattctttc
      241 aaacgcaaca gctcgttttt gaaatagtcg gcaaaatgaa ttgtgtgttt cttctcaaaa
      301 accgtgatgt ccacaaattg gtgacacatg atttggttaa tgaggtctcg gcgaatggtg
      361 tcatcgtgag acagcaggtg ccccttggtt gtggcgagtt gttgtttggc gatgaggttc
      421 tgataatcca caacgttttt ttcgttctga aaataattgc cgtttacaaa agagatgctg
      481 gtggcgccca agccgatttg atgcgcctga gctcgcgtgg aataacccat aaagttgcgg
      541 tgaatggttt tattctttag tgcttggtac agttcatcat ttgttttggc gaagtggtcc
      601 aaaccgattt ggtgatagtt gtgtgcggtc aaaaattgca gtgcctcgga aaaaagtttg
      661 agtttgagtt tgtttgaagg aagatcccgt tcgtcaaaag aacgctgttt gtcctgagtc
      721 cagggcaagt gggcatacga gtaaatcgcg agacggtcgg gatccaaact caaaactgtt
      781 tcgagggttg tttgaaatgt ccgcaaactc tgaccgggca gaccgtagac cagatcaaaa
      841 ttgaaatgcg tgacgccgtg tgttttcagc tgtgacaggg tgttctgggt atcctcgctt
      901 gtctggtggc gatggatcag ctgttggacc tgggcagaaa agtcctgcac cccaagggac
      961 acccggttga atccttcgcg tcccaaaacg gcacagaaag caggtgatgt ggtcttgggg
     1021 ttgatttcga tagcgagctc agcggactgc aacacatcaa aatgcttgtt gatggcgcgc
     1081 atgatgaggg agagctctgt cgggtgcaaa aaattgggag acccccctcc aaaatgaatt
     1141 tgtgtgacag gtgcttttgt gttctgaaga ttctgggcga ccagttcgag ttccttgatg
     1201 acagtgtgaa cgtagttggc gctgagggag tgatctgttg tggcgatgcg ggaacacgcg
     1261 caaaaatgac actggttttc gcaaaacggg atgtgaacgt acaggctcaa ctgatcatcc
     1321 cgtcgtatct ggcttagact ggtctgataa tctgcggcgg tcacctggtt ggcccagacg
     1381 gggagggtgg ggtagctggt gtatcgtggc cccgcgacgt cgtatttgtt aagcagttca
     1441 atgacactga gcatggtggc ctctatatga cagaaacaaa tattttttgt cgatgggaaa
     1501 tgtggggtca aagtcactca atggcgcgtt ttgacttttc gggtcctgct ccgggtcgct
     1561 attttttgcc gctcttcgca agcagggctt gct
//
LOCUS       DATDUN010000227         1377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_301353, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000227 DATDUN010000000
VERSION     DATDUN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301353"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VJC18_02990"
     CDS             complement(<1..761)
                     /locus_tag="VJC18_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLD44378.1"
                     /translation="MTLKTNHHIRNIAIIAHVDHGKTTLVDHLFRQSGLFTKHQQLRT
                     KDRVMDNLDLERERGITIAAKNCSVVYNNIKINIIDTPGHADFGGEVERSLKMADGAL
                     LLVDASEGPLPQTRFVLYKALAAKLKIIVVINKIDRSDARAKEVLNEIYDLFIDLGAD
                     DEQLDFPVYYAIGRDGVAKRTLDGIEQDLKVLFDGMLSYISGPTYDDEEPFQMLVCNL
                     GYSDYTGRLAIGKVFHGSAQKNDQLMCLGDQGAAQ"
     gene            1136..>1377
                     /locus_tag="VJC18_02995"
     CDS             1136..>1377
                     /locus_tag="VJC18_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HLD44379.1"
                     /translation="MLIAFTHNLQLTDSEEEAEFDKPETINAIVQALNNLGHSVELVE
                     VSGPASRVVSLLESLSPDLVFNTAEGRTGRFREAFYP"
BASE COUNT          317 a          332 c          365 g          363 t
ORIGIN      
        1 ctttgggccg cgccctgatc gcccagacac atgagctggt catttttttg ggctgaaccg
       61 tgaaagactt tgccgatggc caaacggcct gtataatccg aataacccag attgcacacc
      121 agcatctgaa aaggttcctc gtcgtcatac gtggggccgg aaatatagga cagcatgccg
      181 tcgaatagga cttttaaatc ctgctcaatc ccgtcgagcg ttcgtttggc tacgccatca
      241 cggcctatgg cataatagac gggaaagtcc aattgttcat catcggctcc cagatcgatg
      301 aacagatcgt aaatttcgtt gaggacctcc ttggcgcgcg cgtccgaacg gtcaatcttg
      361 ttgatgacga cgatgatttt gagttttgcg gccagggcct tgtacaacac aaaacgtgtc
      421 tgtggcagag gtccctctga ggcatccacc agtaagagcg ccccatcggc cattttgagg
      481 gatcgttcaa cctcacctcc gaagtcggca tggcccgggg tatcaataat gttaattttg
      541 atattgttat agaccacaga gcagtttttg gcggcgatcg taatgccacg ttcgcgctcc
      601 agatccaggt tgtccatgac cctatctttg gtcctgagtt gctggtgctt ggtgaacagg
      661 ccgctttggc gaaacaggtg gtcgaccaaa gtcgttttgc catggtccac gtgggcaatg
      721 atggcgatgt tgcggatgtg atggttggtc ttgagcgtca tggagacggg cttctaccag
      781 aaataaaagc gaaatggcaa cgggaatatc acaaaacggg gtcttgttgg tctccccgaa
      841 ttgcttttta cacgtctgcg aaggcatctt catattttta ttgggttgag ccattttgct
      901 atagaacgtc ctgaaaatgt gtttgctacg cattgttctc gcaaaatggc tcctgtcgat
      961 ggtgctgtcc tcgacgtttc aaaagcaatt cggggagacc aacaaaatat ttcggccatg
     1021 gtacaaaatt ttgcggataa cctgttgtgt cgccattgag agtgtggtac ttttttctcg
     1081 gtgacagggc gtgttggggt caacacgtgg tcgccacaca gcaacaggaa cataagtgct
     1141 catcgcattt actcacaatc tccaactcac agacagtgag gaagaggcgg aatttgacaa
     1201 acccgaaaca atcaacgcca tcgttcaggc cttaaacaat ctgggacatt ccgtggagct
     1261 ggtcgaggtt tccggtcctg cgtcacgggt cgtttccctg cttgagtctc tcagtcccga
     1321 tcttgtcttc aatacggctg aggggaggac ggggaggttt cgcgaggctt tttatcc
//
LOCUS       DATDUN010000228         1939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_136171, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000228 DATDUN010000000
VERSION     DATDUN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136171"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1939
                     /locus_tag="VJC18_03000"
     CDS             <1..>1939
                     /locus_tag="VJC18_03000"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44380.1"
                     /translation="DDGKYLVTTGFHNLQGLLTKTSHPREAGSAFQAPQGTLGVANPM
                     TQTAYDVFGKTISHTKDATLGTGSQAYTYAQPRVESFTDEEGYQKRVTRDAHGRAIKV
                     ERGKSGTYSTLATYAYDPANNLVSFTDGEGVQYTYSYDGASRLRAVKWSALSGEDNWY
                     ENTYRGTRKTRMQDLSGGYTNWEYDAIGRLKTMTVSDSLPDSPTGITTYEYTYDDEWI
                     GALTKTTDPSGTTEYTYDEFGRSANVSRSYLATDVSGTESLTAEFVYDYDLRGNIVSK
                     VYPSGRALTMEYDYGFLKKTTSTTSGAEDYSISYDYNKWGLLEKALSSHGHSFTNTFT
                     TPLWVDNIAMTFGGTTYSRTFTWHKNGLLQKYKIDSGVAENTGLGNNALPKTKTKITT
                     MSLSGTLTQGMGATKATAKNTATASSAKSAGVSAGISAEVASANDVHYQYAYDNLKQV
                     ITLTKEALSTVNAQAVEEYTYNTSGSITSSTDHSGKEWTYTYDTGKNRIAGRTSSAGD
                     SEEFVYDNAGRVKTWRSVNGTYTYYYDGQGRLRGMVEGASWKLVLDYDASGALVRRAN
                     NNPYTSSPDYVYSFDAWRYDGKTKQVTELDNNLVSMEGGAQKWVLNNYDGQAARVFGQ
                     TGSALSERVVGVFGTDLTQTG"
BASE COUNT          507 a          477 c          557 g          398 t
ORIGIN      
        1 gagatgatgg caagtatctg gtgaccactg gttttcataa tttgcaggga cttctcacga
       61 agacctcgca tccccgagag gcgggcagtg cctttcaggc accgcagggg actctgggcg
      121 ttgccaatcc gatgacacag acagcctatg atgtttttgg caaaacgatc agtcatacaa
      181 aggatgccac actgggaacg gggtcgcagg cttacaccta cgcgcaaccc cgtgtggaat
      241 cctttacgga tgaggagggg tatcaaaaac gcgtgacccg tgacgcgcat ggcagggcga
      301 tcaaggtgga gcgaggcaag tctggcacgt acagcacgtt ggctacgtac gcgtatgatc
      361 ccgcaaacaa tctggtgagt tttacggatg gggaaggggt tcagtacacc tattcgtacg
      421 atggggcctc gcgtttgcgc gctgtcaaat ggtctgccct ctcgggagag gacaattggt
      481 atgagaacac ctatcggggc acgcgcaaga cacgcatgca ggatctgagc ggtgggtaca
      541 cgaattggga atacgatgcc attggtcgcc tcaaaaccat gacggtcagc gattccctgc
      601 cggattctcc cacagggatc acaacgtatg aatatactta cgacgatgaa tggatcggcg
      661 ccctcacaaa aaccacagac cccagcggga cgactgagta cacctatgat gaatttggca
      721 ggtccgcgaa cgtgtcacgg agctatctgg ccacagacgt ttcgggaacg gaatccctca
      781 cggcagagtt tgtctatgat tatgatctga ggggaaacat tgtgagtaag gtgtatccct
      841 caggcagggc tctcaccatg gaatatgact atggtttttt gaagaaaacc acctccacga
      901 cttcgggagc agaggattac agcatcagct atgattacaa caaatgggga cttttggaaa
      961 aggccttgtc atcacatggt cacagcttta ccaacacgtt cacaacaccg ttgtgggtgg
     1021 acaacatcgc catgaccttt ggcggcacca cgtattcgcg cacattcacc tggcacaaga
     1081 atggtctctt gcagaagtac aaaatcgact cgggcgtggc cgaaaatacc ggattgggaa
     1141 ataacgcgtt accaaagacc aagacaaaaa taacaaccat gtcactctcg ggcaccttga
     1201 ctcaggggat gggtgcaaca aaggccactg caaaaaatac ggcgactgcc tcgtcagcga
     1261 aatccgctgg tgtgagtgcc ggaatatcgg ctgaggtcgc aagcgcgaac gacgtgcatt
     1321 atcagtatgc gtacgacaat ctcaagcagg tgatcacgct caccaaggag gccttgtcga
     1381 cagtgaacgc tcaggctgtt gaggaataca cctacaacac gtcgggtagt atcacgtctt
     1441 ccaccgatca cagtggcaag gagtggactt acacgtatga tacaggcaaa aacaggatcg
     1501 cgggccgcac cagttccgca ggagattccg aagaattcgt gtatgacaac gcaggtcgcg
     1561 tcaagacatg gcgttctgta aatggcacgt acacctacta ctatgatggt cagggacgcc
     1621 tgcgcgggat ggtggagggg gccagttgga agttggtgct ggattacgac gccagtggcg
     1681 cgctggtgcg tcgcgccaat aacaatccct acacgtcgtc gcccgattat gtgtacagct
     1741 ttgatgcctg gcgctatgat ggcaagacca agcaggtgac ggaactcgat aacaatctgg
     1801 tctcgatgga aggaggggcc caaaaatggg tgctcaataa ttacgatggt caagcggcac
     1861 gggtgtttgg tcagaccggc agtgccctat ctgagcgggt tgtgggtgtg ttcggaactg
     1921 atttaacaca aacgggaga
//
LOCUS       DATDUN010000229         1661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_301652, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000229 DATDUN010000000
VERSION     DATDUN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301652"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..>1661
                     /locus_tag="VJC18_03005"
     CDS             80..>1661
                     /locus_tag="VJC18_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44381.1"
                     /translation="MRILNDKGNVTAAIIAIMLLAILGAGMAYLVAANQQTRIQQVTS
                     DQSFYSVQAGLEFALGQILEDGWAGTDITCHFSGEVVTIARAGGTITVSATEKDAMST
                     HSIDDPSPPAPLECLDVNTGSAYISSSVYLMGITINRNAACPLPLTITDMTGTTWSPN
                     LGELIRTIRIDATNRFSAPPTGSGGTFDFGASDVLINNFALHALDYIYWNANVVNHNF
                     QLHFAYTYDGSPYSKVVDVNFLAENQADCLTWDTASARLTWTGSRWRQVTGTTVQNTC
                     ASPIRLMIYTPSWSPAVPAVNFTALRINGTNVYSGGSANGIPLAVDYIIPASTTRTVN
                     YLDFSDEILSRNHTVVWTFADATAKTQNLNLFASNQQDCLTINTSGAAINPTTPTRLV
                     GLTIQNTCGQDIGMTSLTMSWAGEPARRLDRSIILDTDGSNTYNSNNASGVPVDFGTN
                     DLYLRNEDSAKAITYFQFNNSITPGLEFTLSFTMGDGNTKSVTFTPNTQAQYLTVNTA
                     SASIGGSGSRDLLGITVTN"
BASE COUNT          405 a          506 c          384 g          366 t
ORIGIN      
        1 atgacgctca gcgcgggaga gggctttgga agcatcacgg tcaggaacac aatttttccc
       61 agaaggtttg ccactgccta tgcgaatttt gaatgacaaa ggcaacgtca ctgccgccat
      121 cattgcgatc atgctcctcg ccatcctcgg cgcgggtatg gcttatctgg tagcggccaa
      181 ccaacaaaca cgcatccagc aagtcactag cgaccagtct ttttacagcg tgcaggccgg
      241 gctggaattc gcgctgggtc aaattctcga ggatggctgg gccgggacgg acatcacctg
      301 ccatttttct ggtgaggtag tgaccattgc ccgcgccggt gggaccatca cggtctctgc
      361 taccgaaaaa gacgccatgt ccactcattc tatagacgac ccctctcccc ctgcccctct
      421 cgaatgtctc gacgtcaaca cgggctccgc ctacatcagc tcaagcgttt atctgatggg
      481 gattacgatc aaccgcaacg ccgcgtgtcc cctgccactg accatcaccg acatgaccgg
      541 caccacatgg tcccccaatt tgggagagct catcagaaca atccgcatcg atgccaccaa
      601 tcgtttttcg gcccctccca ctggctccgg gggcacattt gattttgggg cgagtgacgt
      661 tctgatcaat aatttcgccc ttcatgcctt ggactacatt tactggaacg ccaatgtcgt
      721 taaccacaat tttcaactgc atttcgctta cacttacgat ggctccccct attccaaagt
      781 tgtcgacgtc aactttctag ccgagaatca ggccgattgc ttgacttggg acacggcaag
      841 cgcacgactc acgtggaccg gtagccgttg gcgacaggtc acgggaacca cagttcaaaa
      901 cacctgtgcc tcacccatca ggctcatgat ttataccccc agctggtccc ccgcggtacc
      961 cgcggtgaat tttacggcgc ttcgtatcaa cggcaccaac gtctattcgg gggggtcggc
     1021 caacggcatt ccccttgcag ttgattacat catccccgcc agcacgacga ggactgttaa
     1081 ttatctggat ttcagtgacg agatcctgtc tcgcaatcac accgtggttt ggacgtttgc
     1141 cgacgccaca gccaaaaccc agaacctaaa tctctttgcc tcaaaccaac aagactgtct
     1201 gacgatcaac acgagtggcg cggccatcaa ccccaccaca ccaaccagac tcgtgggttt
     1261 aaccattcaa aatacctgtg gtcaagacat cgggatgaca agccttacaa tgtcttgggc
     1321 cggagagccg gctcgcagac tggacaggag catcatcttg gacacagatg gttcaaacac
     1381 atataacagc aataacgcca gcggcgtccc cgtggatttt ggcaccaatg atctgtacct
     1441 cagaaacgaa gacagcgcaa aagccattac ttattttcaa ttcaacaaca gcatcacgcc
     1501 ggggcttgaa ttcaccttgt ccttcacgat gggagatgga aatacaaaat ccgtcacgtt
     1561 cacgccaaac actcaagctc aatacctgac tgtcaacaca gcgtcagcct ccatcggcgg
     1621 atccggaagt cgcgatcttt tagggatcac tgtcacaaac t
//
LOCUS       DATDUN010000230         1598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_95597, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000230 DATDUN010000000
VERSION     DATDUN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95597"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1598
                     /gene="ptsP"
                     /locus_tag="VJC18_03010"
     CDS             <1..>1598
                     /gene="ptsP"
                     /locus_tag="VJC18_03010"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HLD44382.1"
                     /translation="YEYLKDHFIRGIPAVPGIAMGQLKIVAHEFAKRFDASSKQNIQI
                     EIDRLKEAFLQVSFEIRETKKQAEAKFGPDEASIFEAYLLFLESNNFQQQIINQIKKG
                     ESAVKALEIIVQQYMDRMSLAHDEYIKERAYDIQDISRKIGDFLLYGESSKMNSFNIG
                     EDTIFFNDFWSISDFINLDLKKTKGIISTTGGANSHISVLADTLNLPTVLGLHSLPTQ
                     LKDGDYIIVDGYAGSVIVNPTKTTVDLYKHELDELNKRTKIFSAYKNKHIRLGADHKR
                     PLMIGANLGMVVHAKTALEHGADMVGLYRTEFPFLIRKQLPTEEEQFLIYKRTLDIMK
                     DRHVVFRTLDIGGDKYVAYLNLPKENNPSLGWRAIRFSLERKDLFRIQLRAILRASRY
                     GKIKLLFPMITSIEQVQEIKVILESVKKELSEEGIAYARNIPLGIMIEVPAAVTIADK
                     LAEEVDFFSLGTNDLVQYCLAVDRSNPLVAKLYDPYHPAVVRMIAQTIRAAHHKGIKV
                     TVCGDTASQPLLAALLIGLGVDNLS"
BASE COUNT          449 a          438 c          344 g          367 t
ORIGIN      
        1 tacgaatatt tgaaagatca tttcatcaga gggatccctg ccgttcccgg aattgcgatg
       61 ggccaactga aaatcgttgc acatgaattc gccaaacggt tcgacgcgag ttccaaacaa
      121 aatattcaaa tcgaaatcga cagactcaag gaagcctttt tgcaggtaag tttcgaaatc
      181 cgcgaaacaa aaaaacaggc tgaggccaag ttcggaccag acgaggccag catttttgag
      241 gcctatctcc tttttttgga aagcaataat ttccagcaac aaatcatcaa tcagatcaaa
      301 aaaggggagt ctgcggtcaa ggccctcgaa atcatcgtcc agcaatacat ggatcgcatg
      361 tcgctggctc acgatgaata catcaaagaa cgcgcgtatg atattcaaga catctcccgc
      421 aagatcggcg atttccttct ctacggagaa tcctcaaaaa tgaattcttt caacattgga
      481 gaggacacca tctttttcaa tgacttttgg tccatatccg atttcatcaa cctcgatctc
      541 aaaaaaacca agggcatcat ttcgacaaca ggcggggcca actctcatat ctccgttttg
      601 gcagacacat tgaatttacc cacagtcctc ggtttgcact ctcttcccac gcaattgaag
      661 gacggagatt acatcatcgt ggacgggtat gcgggtagcg tcatcgtgaa cccaaccaaa
      721 accactgtcg atttatacaa acacgaactc gatgagctca acaaacgaac aaaaatattc
      781 tccgcttata aaaacaagca catccgcctc ggggcagatc acaagcgccc cctcatgatc
      841 ggtgccaact tgggaatggt ggtgcacgcc aaaacagccc tggaacacgg tgccgacatg
      901 gttgggcttt accgcacaga atttcctttc ctgatccgaa aacagttgcc cacggaagag
      961 gaacaatttc tcatttacaa gcgaaccctc gacatcatga aggaccgcca cgtggtgttc
     1021 cgcacattgg atatcggcgg cgacaagtat gtggcttatc tcaatctgcc taaagagaac
     1081 aacccctctc tgggctggcg cgccattcgc ttttcactcg aacgcaaaga cttgttcagg
     1141 attcaactgc gcgccatttt gcgggcctcc cgctacggca aaataaaact cctctttccc
     1201 atgatcacgt caatcgaaca ggtccaggaa atcaaggtca ttcttgaatc agtgaaaaag
     1261 gaactttccg aggagggaat cgcctacgcc cgcaacatcc ccttgggaat catgatcgaa
     1321 gtccccgccg ccgtcaccat cgccgacaaa ttggccgaag aggttgattt cttttccctg
     1381 ggaacaaacg atctggtgca gtattgtttg gctgtggacc gcagcaatcc tctcgtagcc
     1441 aaactttacg atccttacca ccccgccgtg gtcagaatga ttgcgcaaac catacgcgcc
     1501 gcccatcaca aaggcatcaa agtcaccgtg tgcggagaca cggcctctca acctttgctg
     1561 gcagctctct tgatcggtct tggtgtcgac aacctttc
//
LOCUS       DATDUN010000231         1187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_550360, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000231 DATDUN010000000
VERSION     DATDUN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550360"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VJC18_03015"
     CDS             complement(<1..979)
                     /locus_tag="VJC18_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLD44383.1"
                     /translation="MTLFFYVRIDPRMPPTLIIGAGIVGLHVAQALHQKGHEVFVLEE
                     SPYLAEHTSGRNSGVAHTGIYYETGSLKEKLCCEGNQLTHEWLRKLKVTHLECGKWVI
                     ANPGHEDELEIFYKRISPLPIPQPVLKTAAQIKSEEPNLRPTPAIFVPSAFVMDAAGY
                     VKALANFVATLGVQIIQPCRVTGVQDNTLSTTRGPIQFDLAINCAGLFCDDIAKMTGI
                     TNYQIKPCRGDYYVASKQLTHRPVYHLPQKGDHALGIHFTPTVDGQTLIGPNAFFIDD
                     KTDYHHRSEVTPFMTALAEDLPGCEKINLTKGFSGNRPRLFKEGKPVTDF"
BASE COUNT          271 a          272 c          333 g          311 t
ORIGIN      
        1 caaaatcggt aacgggtttg ccttctttga acaagcgagg cctgttgcct gaaaagccct
       61 tcgtcagatt tattttctca caccccggca aatcttctgc cagtgccgtc atgaaaggtg
      121 tcacctctga tcgatggtga taatcggttt tgtcatcaat gaaaaaagcg ttgggaccga
      181 tgagcgtttg tccatcaacc gtgggagtga agtgaatgcc cagcgcgtgg tcaccctttt
      241 gtggcagatg atacacgggt cgatgggtga gctgtttgct ggccacataa taatctcctc
      301 gacacggctt gatctggtag ttcgtgatgc ccgtcatttt tgcgatgtca tcacaaaaca
      361 gaccggcgca gttgatggcg agatcaaatt gaatcggccc gcgcgtggtg ctgagcgtgt
      421 tgtcttgcac cccggtgacc ctgcagggct gaatgatctg cacgccgaga gtggcaacaa
      481 aattggccag agcctttacg tagcccgccg cgtccatcac gaaggccgaa ggcacaaaga
      541 tggcgggggt ggggcgtaaa tttggctctt ctgatttgat ctgcgcggct gttttcaaga
      601 caggttgagg gatgggtaat ggcgaaattc ttttgtaaaa aatttcgagt tcatcttcgt
      661 gaccggggtt ggcgatgacc catttgccgc attcgagatg ggtaaccttc aatttcctaa
      721 gccattcgtg tgtcagctgg ttaccttcgc aacagagttt ttccttgaga gagccagttt
      781 cgtaataaat gcccgtgtgg gccacaccgc tgtttctgcc gctggtgtgt tcggccagat
      841 acggcgattc ttctagcacg aagacttcgt gtcctttttg atgcagggct tgggccacgt
      901 gcaagccaac gattccggct ccgatgatga gggtgggggg catgcgggga tcaatacgga
      961 catagaaaaa taatgtcaaa gcaggtgttg ttctgggtgt gctccttatg gggtgctcga
     1021 aactctgtac tccaaagcca gaactgagaa aaaagtaatc gtcgagtttt ctctcatagg
     1081 actcctctca cgctcgaaaa ggggacttct cttgcgattg cggacactcg aatatgcaga
     1141 tttcgttctc ttgagagtac gatgagggaa aacagagtac agaattg
//
LOCUS       DATDUN010000232         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_52638, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000232 DATDUN010000000
VERSION     DATDUN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52638"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VJC18_03020"
     CDS             <1..637
                     /locus_tag="VJC18_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44384.1"
                     /translation="VIGSRIASYIWSACRSNGSGSSLKNILYADSTESCAISSGSGST
                     DSSMTLMATGTGTLQVFVADCAPIVLDGLTIQSGQTLSVNAQCTALSADIAPGDVTNN
                     SDNASSSSTESTAVTCDGISGLIATNSPADPSLSDYVTVTATTIPPASGCSVNYSMSG
                     TDGYAQSATLTTDAAGAISFTIPPAEIEGIVDTVITTESASGKSSTITYVF"
     gene            complement(657..1355)
                     /gene="pgeF"
                     /locus_tag="VJC18_03025"
     CDS             complement(657..1355)
                     /gene="pgeF"
                     /locus_tag="VJC18_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HLD44385.1"
                     /translation="MTKLFKSGILDEFHHLMHGFTTRELNNDYDRIAQEFGILPSQIY
                     ALKQIHSNQIVVLERGMDLVDIPPGDAYVTSRKDVVIGIKTADCVPILVYEVKQDVVA
                     AIHAGYKGMLAGVIEKAIHHMTDHLSCQVEDMRVAIGPCISVDHYEVSPEIFQDFINV
                     YANNVCCQTYATARPHLDLRSTAKNILEGLGIHPEHIDVSKRCTYEEQDLFFSHRREP
                     ENKGRQFNFIGMVS"
     gene            1428..>2069
                     /locus_tag="VJC18_03030"
     CDS             1428..>2069
                     /locus_tag="VJC18_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44386.1"
                     /translation="MQINKINHVTTLTLSALAFLILFSYGIARPAAESLFLKAHGNEA
                     LPHVWLWLTVVMLAVAALYNHLLCRISLVPLFGAVAVFFALLPAFFIVLVKAEIPGSH
                     YALYIWKDVYIIVLVEIFYTFLNSVFPIKLARWYYGFFGMIAALGGSVASFLVGPLAK
                     WGGTRNSLWLVTATLLLCAALCWPLSRSALNISPSSFKNKRTHLINACQVVRQS"
BASE COUNT          458 a          564 c          502 g          545 t
ORIGIN      
        1 tgtcatcggc agtcgcattg cctcctacat ctggagtgcc tgccgttcca acggttccgg
       61 cagttcgtta aagaatatcc tctatgctga cagtactgaa tcctgtgcca tcagctctgg
      121 gtccggatca acagattcct caatgaccct gatggcaacg ggaacaggga cgttgcaggt
      181 ttttgttgca gactgcgccc ccatcgttct tgacggcctg accattcaga gtggtcagac
      241 cctgagtgtc aacgcacaat gcactgccct gtcggcagat attgcaccag gcgacgtcac
      301 caacaattcc gataatgcca gttcttcatc aacagaaagt acggccgtga cctgtgatgg
      361 catctcggga ctcattgcta caaacagtcc tgcagatcct tccctgtccg attatgtcac
      421 ggtaacagcc accaccattc cgccagcctc aggctgtagt gtaaattaca gcatgtcggg
      481 cacagatggc tatgcgcaaa gcgccaccct cacgacagat gcggcgggcg ctatttcttt
      541 tacgattcct ccagcagaga ttgagggtat cgtggatacc gtcatcacca cggagtccgc
      601 ctcgggaaag agcagtacca tcacgtacgt tttctgaggc atcttttgtt gactggtcaa
      661 ctcaccatcc caataaaatt gaattgtcgg cccttgtttt cgggctcgcg gcggtgggaa
      721 aaaaacaggt cctgctcctc gtaggtacag cgcttgctga catcgatatg ctcaggatga
      781 atccccaatc cttccagaat gttttttgct gtgctcctta aatccagatg aggacgcgca
      841 gtggcgtacg tttgacagca aacattgttg gcataaacat tgataaagtc ctgaaaaatt
      901 tctggactca cttcgtagtg atccacagaa atgcagggac ctatggccac ccgcatgtct
      961 tcgacctgac acgacaggtg atcggtcatg tgatgaatcg ctttttcaat cacaccggcc
     1021 agcatgccct tgtaaccagc gtgaatggcg gccaccacat cttgtttgac ttcataaacc
     1081 agaatcggca cacaatccgc tgttttgatg ccaatgacaa catctttgcg gctggtcaca
     1141 taagcatccc ccggggggat atcaaccaga tccatccccc gctccaacac aacaatctgg
     1201 ttggagtgga tttgcttgag ggcatagatc tggctcggca gaatgccaaa ctcctgcgcg
     1261 atcctgtcgt aatcattgtt caactctctg gtggtgaatc catgcatgag gtggtggaat
     1321 tcgtcgagga ttccggattt gaacaatttg gtcatgagag cagtttttat atttgaagag
     1381 ggtccgtttg acaatcgtaa agatgatcag ttacacctca cgttgttatg caaataaaca
     1441 aaataaatca cgtcaccact ctcacccttt ctgctttggc atttctgatt ttattttcct
     1501 acggcatcgc ccgccctgcc gctgaatccc tgtttctcaa ggcgcacggc aacgaggcac
     1561 tgccacacgt ttggctatgg ctgaccgtgg tgatgctcgc cgttgccgct ttgtacaacc
     1621 acctcttgtg cagaatcagt ctggtccccc tgttcggcgc tgtcgcggtt ttttttgccc
     1681 tcctgccggc ttttttcatc gtgttggtca aggctgagat ccccggcagt cattatgccc
     1741 tctacatctg gaaagatgtc tacatcattg tgctggtgga aattttctat acttttttga
     1801 attccgtctt tcccatcaaa ctggcgcggt ggtattacgg gttctttgga atgatcgccg
     1861 cgttgggagg aagtgtcgcc agttttctgg ttgggcccct ggccaaatgg ggaggcacac
     1921 ggaattcact gtggctggtg acggccacct tgttgctgtg cgcggcgctc tgctggccct
     1981 tgtcccggag cgcgctgaac atcagtccgt cctcattcaa aaacaaacgc acccacctca
     2041 tcaatgcctg ccaggtggtc aggcagagt
//
LOCUS       DATDUN010000233         3809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_426892, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000233 DATDUN010000000
VERSION     DATDUN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426892"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /gene="gltX"
                     /locus_tag="VJC18_03035"
     CDS             complement(<1..917)
                     /gene="gltX"
                     /locus_tag="VJC18_03035"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008218106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLD44387.1"
                     /translation="MNQKPIITRFAPSPTGYLHIGGARTALYNYLLAKRYGGKFILRI
                     EDTDLERSTDESIKAILDGMTWLGLNWDEGPYYQTKRFDLYNQAVERLVREGKAYPCF
                     CSAEKIAAQREATQADKETFRYDRACYHLSEDERKRRIEAGEPHCIRFYSNDDETIII
                     HDKIKGEVKVEGKELDDLIIKRTDGAPTYNLSVVVDDAAMGITLVIRGDDHLRNTFRQ
                     VQLYKALGLDVPEFAHLPLILGADKKRLSKRHGAMSVMAYKDLGYLPEAIVNYLVRLG
                     WGHGDQEVFTKQEMISAFGLEACSTAAAV"
     gene            complement(1046..2323)
                     /locus_tag="VJC18_03040"
     CDS             complement(1046..2323)
                     /locus_tag="VJC18_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44388.1"
                     /translation="MKNNTRMMLLVVLLLSSVVYLFVSCGGSSTTNADDNDPGVTGTP
                     QYTTIDELPTALTRVVSAETATSLSALVKPKHAIGFGDSYDDVTGAFVPGTTSMAACE
                     TYNQTKWALSQASQGDTVMCYVQHVWEAIAADANHLLHNDDIYDGDWHTYDLVSPEGE
                     ERPDLIRMRLTKDDDGFITGFTMFACKEGVQQMYLNQTIDTTTGEFDMTNISTYESPE
                     GHGSEKAVVSGVVNELGYFEGTKNMTMEFEGQWGTDTSSGHMDFTQTEDGGSINGIMT
                     GEYDNGDSIGTYENKAYSIFELVDSNTGDTYSIANLALGAGSAHYGMSGTCDECGPEG
                     WEDSGTESWSASAGSPVEDSNDYTDSADDVDLLTAEDISEPSFGSNTVDCVALAADTD
                     GEIDFEDLDVNPEAACEQVDHNHVDCWNLLNDQ"
     gene            2612..>3809
                     /locus_tag="VJC18_03045"
     CDS             2612..>3809
                     /locus_tag="VJC18_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44389.1"
                     /translation="MFITNPLLTKLLPHMNHGSPSVTLTGIADTQSLNAFVESVSSFS
                     SARAEHPFPDDPIKFRAQWSLVKTHHICGGVPLNFQNVEELLAWRKEGRFVVPEQFEN
                     TRNDERGALSSIRSQFTDPDICALHISLFNAGFLIQETRPSAERNIGQWHVRELVDLR
                     EGLEVVELLDAHTQGTAYDIMDLIASVDWGDFRPYGLKNINASLSSLSEFKNPHYRHS
                     NGEEETTIITKKCHCVAHVFMGRLTVLWGKTQMLSFQTPPYRQPSAIKHIAAIQARLR
                     YHSFARLAVEIAVANKLKASGLPHYAFIDDTSFDCMLNAIYHMGFDDMTQLSREDYIA
                     LHKKTFEALWTKTSISHEHTLTRIQDMLTLPLSGTAQSHAENTLDTIARKISEDIREM
                     RSDRACI"
BASE COUNT          998 a         1025 c          806 g          980 t
ORIGIN      
        1 aacacagccg ccgccgtaga acaggcctcc aaaccaaagg cagaaatcat ctcctgtttt
       61 gtaaacacct cctgatcacc gtgcccccaa cccagccgca ccagatagtt tacaatcgcc
      121 tctggcaaat accccaaatc cttgtacgcc atcacactca tggcaccgtg gcgcttggac
      181 aatctttttt tgtcggcccc caaaatgagt ggcaaatgcg caaactcagg aacatccagc
      241 ccaagcgctt tgtaaagctg aacttgacgg aacgtgttgc gcaagtggtc atcgcctctg
      301 atcaccaatg tgatccccat agcggcatca tccacaacaa ccgacaaatt atatgtggga
      361 gcaccatcag tccgtttgat gatcagatca tcaagctctt ttccttcgac tttcacttcg
      421 cccttgattt tatcgtgaat gatgatggtc tcgtcgtcat tggaataaaa gcggatacag
      481 tgcggttctc ccgcttcgat gcgccttttc cgttcgtctt cactcaaatg ataacaggcc
      541 cgatcataac gaaatgtttc tttgtctgcc tgtgtggcct ctctttgtgc cgcaattttt
      601 tcggccgaac aaaaacaggg atacgctttt ccttcgcgca ccagccgctc cacggcctga
      661 ttgtaaagat caaaccgctt ggtttgataa taagggccct cgtcccaatt gagccccagc
      721 caggtcatgc catccagaat ggcttttatg gattcatcgg tagagcgctc aagatcggtg
      781 tcttcgatgc gcaggataaa cttgccacca tatcttttgg ccaataaata attatacagt
      841 gcggtgcgtg cgccgccaat gtgcaaataa cctgtggggg acggggcaaa gcgggtgatg
      901 attggttttt ggttcataaa gttctcgttt cgtaataaaa gctcattaaa acgtgccggg
      961 gaaaaagatc aaattcgaaa aaaagggcgg tctttgggac cgcccttgaa atacagccaa
     1021 caaatgatca aaccagaagc ctctcttatt gatcattcaa caagttccag caatcaacgt
     1081 gattgtgatc cacttgttca cacgcggcct cggggttgac atccagatct tcaaaatcaa
     1141 tctcaccatc ggtatcagct gcgagcgcga cacaatcaac tgtgttgctg ccaaacgaag
     1201 gctcagaaat gtcctccgcc gtgaggaggt ccacatcgtc agcactatcg gtataatcgt
     1261 tgctgtcttc tacaggagac cctgcagagg cactccagga ttctgtgccg gaatcttccc
     1321 agccttctgg gccacattca tcacatgttc ccgacatgcc ataatgagca gatcccgcac
     1381 ccaaagccag attggcaatg gaataggtgt cgccagtgtt ggaatcaacg agttcgaaaa
     1441 tgctgtaggc cttattttcg taggtgccta ttgaatcgcc attatcatac tcacctgtca
     1501 tgatgccatt gattgagcca ccgtcctctg tctgcgtaaa gtccatatgc ccactgctgg
     1561 tatcagttcc ccactgccct tcgaattcca tggtcatatt cttggtccct tcgaagtaac
     1621 caagttcgtt cacaacccca ctcaccacgg ccttttctga accatgtccc tccggacttt
     1681 cgtaggtcga aatgttggtc atgtcgaatt cacctgttgt ggtatcgatt gtttgattga
     1741 gatacatctg ttgcacgccc tctttacagg caaacatggt aaagcccgta atgaacccat
     1801 catcatcctt ggtgagtctc atgcgaatca ggtccggtcg ctcttcgcct tctgggctaa
     1861 ccagatcgta tgtgtgccag tcaccatcgt agatgtcgtc gttatgcaag aggtggttgg
     1921 catcggcagc aatagcctcc caaacatgct gaacatagca catcaccgta tcgccctgac
     1981 tggcctgact caaggcccac ttggtctggt tgtaagtttc acaggccgcc atgctggtcg
     2041 tgccaggaac gaatgccccc gtcacatcat cgtaggaatc gccaaaacct atcgcgtgct
     2101 tcggcttcac caaagccgat aaacttgtcg ctgtttcggc actgaccaca cgcgtgagag
     2161 ccgtgggcag ttcatcaatt gtggtgtact gtggcgttcc cgtgacccct ggatcattat
     2221 catctgcatt tgtggtgcta ctgccgccgc aagagacaaa caaatagaca acggaactca
     2281 gtaacaaaac cacaagtaac atcatccttg tgttgttttt catttttccc ccttaaattg
     2341 ttaatacaac ttgatcacga ttggcccatg accaagcttg cctgagaagg tctccaaaaa
     2401 atattttcgg tgacctcaaa agagtgggcg aatttttaca ctttttgcat agaatagcaa
     2461 gtttttttaa taaattcata ccattacaac cacacccccc acttcggatt tttctttaca
     2521 aaaccaaaga aaaaaaacac aacattttca cctcgttgcc gataataccc aagagggaat
     2581 tttctgactg tcatactccg aggaaacacg catgttcatc acaaatcccc tgctgactaa
     2641 attacttcca cacatgaatc acggctcgcc ttctgtcacc ctgaccggta ttgccgacac
     2701 acaaagcctg aatgccttcg ttgaatccgt cagttcattt tcatcagcac gcgccgaaca
     2761 cccctttccc gacgatccca ttaaatttcg ggcgcaatgg agtcttgtaa aaacgcacca
     2821 catctgtggt ggtgttcccc tgaattttca aaacgtggag gaactgttgg cttggcgcaa
     2881 agaaggtcgc tttgtagtgc cggaacaatt tgagaacaca cgcaatgatg agcgcggggc
     2941 gctgtcttca atcagatcac aatttaccga tcccgacatc tgcgccctcc acatatcgtt
     3001 attcaatgct ggttttttaa ttcaggaaac aagaccctca gcagaaagaa acatcggtca
     3061 gtggcatgtc agggaactag ttgatttgag agaagggctt gaggttgtgg aattgctcga
     3121 tgcccacaca caaggcacgg catacgacat catggatctg attgcctcag tagactgggg
     3181 cgatttccgt ccttatggcc tcaagaacat caacgcatca ctaagttctt tgagcgaatt
     3241 caagaaccca cactatcgcc actccaatgg agaggaggaa accactatta ttaccaaaaa
     3301 atgccactgc gttgcgcatg tctttatggg tcgactcaca gttctttggg gaaaaactca
     3361 aatgctgagt tttcagacac cgccctaccg ccaaccttct gccatcaagc acattgcggc
     3421 cattcaggca cgcctacgtt atcattcttt tgcaaggctg gccgtcgaga ttgctgtggc
     3481 aaacaaacta aaagcgtctg gtcttccaca ttatgcattc atcgatgata ccagttttga
     3541 ctgcatgttg aacgctattt atcatatggg ctttgatgac atgactcaac tcagccgtga
     3601 ggattatata gcgcttcaca aaaaaacctt tgaggcctta tggacaaaaa cttccatcag
     3661 ccatgaacac acactcactc gcatccaaga catgcttacc ctcccccttt ctggaacggc
     3721 ccagtcacac gcagaaaata ctttggacac aatcgcacga aagatatctg aagacatacg
     3781 agaaatgcgg tctgacaggg cttgtattg
//
LOCUS       DATDUN010000234         3126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_467729, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000234 DATDUN010000000
VERSION     DATDUN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467729"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..519)
                     /locus_tag="VJC18_03050"
     CDS             complement(157..519)
                     /locus_tag="VJC18_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44390.1"
                     /translation="MSSKISFALAVFIGFFFILISHNAIAAEAEAPQSYLSQMEGHME
                     LIEANFQKMLQTQDAQEKSAFLKQHWSLLDKHLELMNQMIDNERDLGLKSTKRRMKYR
                     IHALLDMFKKTQHYLKVP"
     gene            complement(612..685)
                     /locus_tag="VJC18_03055"
     tRNA            complement(612..685)
                     /locus_tag="VJC18_03055"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(649..651),aa:Gly,seq:gcc)
     gene            904..1692
                     /gene="mazG"
                     /locus_tag="VJC18_03060"
     CDS             904..1692
                     /gene="mazG"
                     /locus_tag="VJC18_03060"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HLD44391.1"
                     /translation="MKTYSHLNEFINIIRRLRAPGGCPWDRKQTHKSLKPYVLEETYE
                     VLEAIDSGDPEHLKEELGDLLLQVILHAQVASDQGAFTIEDVAKLVSEKMIRRHPHVF
                     GNAKVKDADEVLTNWVAIKRDEKINKGKQPSVFDGIPKHLPALFENYKISRRVARMGF
                     DWKKPEDVFEKVLEELGETRRAVKKKNKTHIEEELGDLLFTVANLCRAYNVNPELTLR
                     QANKKFMKRFEKIERRLLRDNLDPQKISFKKWNELWERAKKSQT"
     gene            1795..>3126
                     /locus_tag="VJC18_03065"
     CDS             1795..>3126
                     /locus_tag="VJC18_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD44392.1"
                     /translation="MTFVSLIFCLFASAVSAQTPLPNLQKPALKEYQKTLEQIDKTRQ
                     KDIIKLKYTIKSKYVNDAVKLSRARDLIDKKQFDSALSLLKKIKKSSPLSPYAALYTS
                     QALVAKKKYEQALDALPALTDPVTKIEWDLFWQRLNVLALSKQKNTLPALLEPVQKKY
                     KKDKNIALKTNYYLGLAEHIAKNEGTAFDHLRKVVVGLPGTEFDQKIFALLSLKKIEA
                     NKFLSTTQWNERAKNLAATGYTIEALEIWKALAQNSAPHSDYLAQAYYKARIYDQAAQ
                     QYENLFAQNPSLKSNAAYWDKLAHSYLRQNQFTKAHDAFLHIATNFSQTREAGIARFK
                     LGFIYFDSGQYAKAIDYFAKFMDQGSSWMKNRAHWFTFWSHYLLKDYPLALTDVEKML
                     SLQKKMTDTKMSLLYWKARLLEKTSQTDQAKGLYQEVSRLDSLGYYPLMARQ"
BASE COUNT          950 a          782 c          682 g          712 t
ORIGIN      
        1 ttatcgtcta aattacacac aacagaaaac cgacaaaaca ctattgattt gtcgcaatcc
       61 ctgttggggg acgttgtttc gcacagatct tgccgtaaag taaagtgaaa tgttgattga
      121 aatggcaatg cgtcagtcag accatgcagg ccatttttat ggtaccttca gataatgctg
      181 ggtctttttg aacatatcga gcagggcgtg aatgcggtat ttcatgcggc gtttggtgct
      241 tttgaggccc aggtcacgtt cattatcgat catttgattc atgagttcga ggtgtttgtc
      301 cagcagggac caatgttgtt tgaggaaagc acttttttcc tgcgcgtctt gggtttgaag
      361 cattttttga aaattggcct caatgagctc catgtgccct tccatttgtg acagatatga
      421 ttggggggct tcggcctcag cggcgatggc attgtgggaa atcagaataa aaaaaaagcc
      481 gatgaacacg gccagcgcga atgatatttt ggatgacatc atttggtaac gcacaactga
      541 ccccgcaaaa cggggtctaa gaacgactaa gttctccttc gcgaaactca ggagaacaaa
      601 gccgttctaa gagcgggaaa cgggactcga acccgcgaca ttaaccttgg caaggttacg
      661 ctctagccaa ctgagctatt cccgcgtaaa ttttgcttcg caaaatttgg tagttagtag
      721 ttagaagtta gcaatcacaa actacccata ccaaatggag cgaccttatt aactattttg
      781 tttttcttgg caagaaaaat ttcattgcga tacgtagttg attgattggt tttttttaca
      841 tatcgacagc tctccggaac tctatgcctt acgccttaaa ccttgtgcca aacagtcaca
      901 gttatgaaaa cttattctca cctcaacgaa ttcatcaaca tcatccgcag attgcgggct
      961 cccggggggt gtccatggga tcgcaaacag acccacaaat ccctcaaacc ctacgtgctg
     1021 gaagaaacct atgaggtgct ggaggccatt gattccggtg atccagaaca tttgaaagag
     1081 gaactgggag atctgctcct gcaggtcatc ctgcacgcgc aggtcgcgtc cgaccaggga
     1141 gcattcacca ttgaggatgt ggccaaactc gtcagtgaaa aaatgatccg cagacacccg
     1201 cacgtgtttg ggaacgccaa ggtcaaagac gccgatgagg tcctcaccaa ctgggtggcc
     1261 atcaagaggg atgaaaaaat caacaagggc aaacaacctt ctgtcttcga cggcatcccc
     1321 aaacacctgc ccgccttatt tgagaattac aagatttccc ggcgtgtcgc ccggatgggc
     1381 tttgactgga agaaaccgga agatgtcttt gaaaaggtcc tagaagaact gggcgaaacc
     1441 agacgggccg tcaaaaagaa aaataaaaca cacatcgaag aagaactcgg tgatcttttg
     1501 ttcactgtcg caaacctgtg ccgcgcctac aacgtgaatc ccgaactgac actcagacag
     1561 gccaataaaa aattcatgaa acgttttgaa aagattgaaa gacgcctttt aagggacaat
     1621 ctcgatcctc aaaaaatcag tttcaaaaaa tggaatgaac tgtgggaaag agcgaagaaa
     1681 tcgcaaacct aggccaaaat atcaaatatg atatatccca agaaatgtca ttcctgcgca
     1741 cgcaggaatc cagaaaataa ccacaaatgg atccctgcct tcgcagggat gacagtgaca
     1801 tttgtatcgc tcattttctg cctctttgcc agcgccgtct ccgcccaaac tccactgccc
     1861 aacttgcaaa aacccgctct caaggaatac caaaaaaccc tcgaacagat cgacaagacg
     1921 cggcaaaagg acatcatcaa actcaagtac acaatcaaat caaaatacgt caacgatgcc
     1981 gtcaaactga gtcgcgcccg tgatttgatc gacaaaaaac aattcgattc tgccctgtca
     2041 ctgctcaaga aaatcaaaaa gagctcaccg ctgtccccct atgccgctct gtatacgtca
     2101 caggcgttag ttgccaaaaa aaaatacgag caggccctcg atgccctgcc cgccctcaca
     2161 gaccccgtaa ccaagatcga atgggatctg ttctggcaaa gactcaatgt gctggccctg
     2221 agcaaacaaa aaaacaccct gcccgccctg ctggaacctg tccaaaaaaa atacaaaaaa
     2281 gataaaaaca tagccctcaa aacaaattat tatttggggc tggctgaaca catcgccaaa
     2341 aacgaaggaa cagcgtttga tcatttgcgc aaagtggtgg tgggactacc cggaacggag
     2401 ttcgatcaaa agatatttgc tctattgtcc ctaaaaaaaa ttgaggccaa caaatttctt
     2461 tcgacaaccc aatggaacga acgcgccaaa aacctggccg ccacaggcta cacaatcgag
     2521 gcactggaga tctggaaggc actcgcccaa aacagcgcgc ctcattcgga ttatctggcc
     2581 caggcctatt ataaagcaag aatctacgat caagccgctc agcagtacga aaacctcttt
     2641 gcgcaaaacc cgtccctcaa gtccaacgcc gcctattggg acaaactggc ccattcctat
     2701 ttgagacaaa accagttcac caaggcacac gatgcctttc tccacatcgc caccaatttt
     2761 tcacagacaa gagaggcagg gatcgcgcgg ttcaagttgg gattcattta tttcgattcg
     2821 ggccagtacg ccaaggcaat cgattacttc gccaaattta tggatcaggg atcttcctgg
     2881 atgaaaaacc gtgcccactg gtttacgttt tggagtcact acctgttaaa ggattacccc
     2941 ttggcattaa cagacgttga gaaaatgctc agcctgcaaa aaaaaatgac cgacaccaaa
     3001 atgtctctcc tgtactggaa ggcacgcctt ctggaaaaaa ccagtcaaac tgatcaggcc
     3061 aagggacttt atcaggaggt atcacgtctc gattcactgg gatattatcc ccttatggcc
     3121 agacaa
//
LOCUS       DATDUN010000235         1879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_52879, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000235 DATDUN010000000
VERSION     DATDUN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52879"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..469)
                     /locus_tag="VJC18_03070"
     CDS             complement(98..469)
                     /locus_tag="VJC18_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLD44393.1"
                     /translation="MRRQGFTIIELVLVLAILAVLSVSAMPKIFGLTTEARTATTDNM
                     VGAVRTGIYLYHLYYQECPSMLLGLRLGPCSATNACFGNVLKTPVRSQWSVLVRNRRF
                     QAPDGRVFDYNLADDCSFNPL"
     gene            complement(609..1028)
                     /locus_tag="VJC18_03075"
     CDS             complement(609..1028)
                     /locus_tag="VJC18_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HLD44394.1"
                     /translation="MKDQHDPACFFDDGIRFECQQCGACCTGDPGTIYVSAEEIKKIA
                     AHNKRPIKRFVKEYLYPFKDSYSIREHGDGRCYFFETGRGCTIYAVRPQQCRTFPFWF
                     SNLRNEAYWQVIANGCPGIGKGRCFSKGEIIEQINSF"
     gene            1076..1843
                     /locus_tag="VJC18_03080"
     CDS             1076..1843
                     /locus_tag="VJC18_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014769600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HLD44395.1"
                     /translation="MTYSYLQIIFLFACAFFAGLVDTIAGGGGIITIPALLAVGIPPH
                     LALGTNKLQSSFGSLTATIRFARQGLVRTESLLAGILITFVSACLGTICIQHMSSDIL
                     KLIMPLMLAGVFLFLIFAPRFGLEDQKPRLGALGFYALFGTLIGFYDGFFGPGTGTFW
                     ALSLAFFLGMNLKTATANTKVLNNTSNVASLLFFLLGHQVLFGLGLLMAVGQVLGAMV
                     GARLVIRHGAHFVKIIFLFVVGGTLCKVVWSTFFAAR"
BASE COUNT          441 a          471 c          416 g          551 t
ORIGIN      
        1 caagataatt cgtgatactc ggaaacagtc aaatcttatt gtgtatttat tcatgttcat
       61 cattgatcat aataaggaaa aaccgattga ccgattgtca gaggggattg aaactgcaat
      121 catcggcaag attgtaatca aaaacacgtc cgtcgggggc ttggaatctt ctgtttcgta
      181 ccaaaacgct ccactgggat cgcacaggcg ttttgagcac attcccaaaa caagcatttg
      241 ttgctgaaca aggtcccaat cttaatccta ataacatcga agggcactcc tgataataga
      301 ggtgataaag ataaatgcct gtacgaacgg cccccaccat attgtcggtt gtcgcagtcc
      361 gcgcttcggt tgtcagacca aatattttgg gcatggcaga taccgacaaa acagctaaaa
      421 ttgccagcac caaaaccaac tcaatgatgg tgaaaccctg tcgtctcatt cccccctcca
      481 tttttctttt ttagacagtc cttaacaaca caagacagcc ttatcaacaa cggcagacac
      541 agcatttcca tggtatcact acagaggttt gtgtgtaaag aaatagacac tgcctttctg
      601 tgcctttctc aaaacgaatt gatctgttcg ataatctccc cttttgaaaa acatctccct
      661 ttgccaatgc ccggacagcc attggcaatg acttgccagt aggcttcgtt tcgcaggttt
      721 gagaaccaaa acgggaacgt acgacactgc tggggccgca cagcatatat cgtgcaccct
      781 cgacctgttt cgaaaaaata acagcgccca tcaccatgtt ccctgattga ataactgtct
      841 ttgaagggat acaggtattc tttgacaaac ctttttattg gccttttgtt gtgtgccgca
      901 atttttttga tctcttctgc cgagacataa atggttcccg gatcacccgt gcaacaggcg
      961 ccacattgct ggcattcaaa cctgatgcca tcgtcaaaaa aacaggcagg gtcatgttga
     1021 tctttcatgc cattcacatt aatcttccta cgttcctttg caagaatgat ttttcatgac
     1081 ttattcctat cttcaaatca ttttcctttt tgcctgtgcc ttttttgccg ggttggtcga
     1141 tacgatcgcg ggcgggggag gcatcatcac catccctgcc ctgctggccg tgggcatccc
     1201 acctcatctg gcgctgggca ccaacaaatt acagtcttcc tttggcagtc tgactgccac
     1261 cattcgtttt gcgcgccagg ggctggtacg cacagaatcc ctgctggcag gaattctgat
     1321 tacctttgtg agcgcctgtc tgggcactat ttgcatccag cacatgtcat ccgacatcct
     1381 caagctcatc atgccgctca tgctggcggg agtctttttg tttttgatct ttgccccgcg
     1441 ctttggcttg gaggatcaaa aaccacgcct tggcgccctt ggattttatg ctctctttgg
     1501 aaccctgatt gggttctatg atggtttttt tggtccgggc acgggaacct tttgggccct
     1561 gtctctggcg ttttttttgg gaatgaacct caaaacagcc acagccaaca ccaaggtgct
     1621 caacaacacc agcaatgtgg cctcgttgct tttttttctg ctgggtcatc aggtcctgtt
     1681 tggtcttggg ctcttgatgg ctgtgggaca agttcttggg gcgatggtcg gagcccggct
     1741 ggtcattcgt catggtgccc attttgtgaa aattattttt ttatttgtcg ttgggggcac
     1801 cctgtgtaaa gtggtttggt caactttttt tgccgcgcgc taacaacctg ttacaacgtg
     1861 cgggtggaga ttcaaaaat
//
LOCUS       DATDUN010000236         1875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_550914, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000236 DATDUN010000000
VERSION     DATDUN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550914"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VJC18_03085"
     CDS             complement(<1..306)
                     /locus_tag="VJC18_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44396.1"
                     /translation="MDRQINFSIHDPRPSAHKLMCGIAGIISLKEPGVDRLWLKKMND
                     TLSLRGPDDEGFYFSDHVGLAHRRLSIIDLASGHQPMTTEDGRFSLVFNGEIYNFLEI
                     "
     gene            complement(327..1298)
                     /locus_tag="VJC18_03090"
     CDS             complement(327..1298)
                     /locus_tag="VJC18_03090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLD44397.1"
                     /translation="MVATKEKKYDLSILIPFFNEEESLVENYNNLKAVIENLAVSTEV
                     IYIDDGSTDRGLSVLRELATRDPHLKIVSFSRNFGQTAAMEAGFKTASGDIYITLDAD
                     NQNDPNDIPTLLEKMNEGYDVVSGWRKNRKDKFLRRVLPSKIANWLISKVTGVRLKDY
                     GCTLKAYRSYYLDQVHLYGEMHRFIPAYAKYAGAKVTEAAVNHRARTKGTSKYGLSRT
                     FKVILDLITVKFLGDYATKPIYFFGGLGALFAFASFLVVSYVLYQKFFFTEPVMVHKN
                     PLFLLSLFIFMLSVVLVMMGLLAELLMRTYHESQDKKTYRIKETIGF"
     gene            1433..>1875
                     /locus_tag="VJC18_03095"
     CDS             1433..>1875
                     /locus_tag="VJC18_03095"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HLD44398.1"
                     /translation="MKPKVFVTGCAGFIGSHYCEALLRQGYPVIGLDNFNNFYDPNVK
                     ENNVLAVQQTAAKQGGTFVLIRGDIRDQGLIQRSFLDHKPAILVHLAAMAGVRSSLEK
                     PALYSDVNNTGTVVVLEGARQAGIVSVVFASSSSVYGNGLTVPFS"
BASE COUNT          502 a          412 c          455 g          506 t
ORIGIN      
        1 gatttctaaa aaattataaa tttctccgtt gaagacgagg ctaaaacggc catcttctgt
       61 ggtcatgggt tggtggccac tggcaaggtc tatgatagac agccttctat gcgccaaacc
      121 cacatgatct gaaaaataaa agccctcatc atcgggtcca cgaagagaga gggtgtcgtt
      181 catttttttg agccaaagac gatccacgcc aggttctttg agcgagatga ttccagcaat
      241 gccacacatg agcttgtggg ctgaggggcg tgggtcgtgg atcgagaaat ttatttgtcg
      301 atccacagac aacaagccac cagcgcctaa aaacctatag tctctttgat acgatacgtt
      361 tttttgtcct gtgactcatg gtatgttcgc atgagcaact cggcaagcaa ccccatcata
      421 accaaaacca cgctcaacat aaaaatgaaa agcgatagca aaaacaaggg gttcttatga
      481 accatcacag gttcagtgaa aaagaacttt tgatagagaa catatgaaac caccagaaaa
      541 gaagcgaagg caaacagggc ccccaggccc ccaaaaaaat aaatgggctt tgtggcgtaa
      601 tctcctaaaa acttcaccgt gatgagatcc aggattacct tgaacgtacg gctcaggcca
      661 tattttgatg ttccttttgt acgcgccctg tgattgacgg cggcctccgt gaccttggcc
      721 ccggcgtatt tagcataggc gggaatgaat cggtgcattt cgccgtacag gtgtacctga
      781 tctagataat atgagcgata ggccttaagg gtacaaccat aatctttgag tcggacccct
      841 gtgaccttgg aaataagcca gtttgcaatt ttcgaaggga gtacacgtct cagaaatttg
      901 tcttttctgt tttttctcca accactcacc acatcatagc cttcgttcat tttttccaac
      961 aatgtgggaa tgtcattggg atcgttttgg ttgtcggcat cgagcgtaat atagatatca
     1021 ccggaggcgg ttttaaagcc ggcctccatg gccgcggtct gaccaaaatt acgcgaaaaa
     1081 gaaacgattt ttaaatgagg atcccgggtg gccaactcgc gcaacaccga caaaccccga
     1141 tccgtggatc cgtcatcgat gtagatgacc tctgtggaga cggccaggtt ttcaatgacg
     1201 gcttttaggt tgttatagtt ttcaacgaga ctttcttcct cattgaaaaa aggaatgagt
     1261 atagataaat cgtatttctt ttctttggtt gctaccatct tgcagctctg atagcagaga
     1321 aagccataaa gatgcaaatt tgaaatgaga agcttccctg ttgacacaca gaccaggggg
     1381 gctgtgttag caggaggcat gcaaccaaaa acatcgtgaa atgtacaagg gtatgaagcc
     1441 taaagttttt gttacaggat gtgccggatt tatcggctcc cattactgtg aggctctgtt
     1501 gcgacagggc tatccggtaa tcggccttga taatttcaat aatttctatg atcccaatgt
     1561 gaaggaaaat aatgtattgg ctgttcaaca gaccgcagca aagcaggggg gtacttttgt
     1621 tttgatcagg ggtgatatcc gtgatcaggg gctgattcaa cgttcgtttc tagatcataa
     1681 gcctgctatc ttagtccatt tagcggctat ggcgggggtg cggtcttctc ttgaaaagcc
     1741 ggcgctgtat tccgatgtga acaacacggg aacagtggtt gtgctcgagg gcgcgcgtca
     1801 ggcggggatt gtttctgtcg tttttgcttc ttcatcttcg gtttacggaa atggcctcac
     1861 tgttcctttt tccga
//
LOCUS       DATDUN010000237         5756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_509731, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000237 DATDUN010000000
VERSION     DATDUN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509731"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1614
                     /locus_tag="VJC18_03100"
     CDS             208..1614
                     /locus_tag="VJC18_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HLD44399.1"
                     /translation="MIGTARPVTTLTSPAFALPNVYLRHADPDALRAMGFPNEAPDVY
                     RQKNTVFFNYWLRDAGRLSPLFPGPERTRRFLRRLVERLPYGNEALRFLRGPAHQDAS
                     TVPGIERVLLDNEGKADPNSAKVLRREGQAPTGDTINDKLFFNAGLAVKQANGLITEV
                     NKTLARMYPALTVDINMLRGALILCINHDPEQADNLGWPNAAAVPIEKGVGLVLFGLL
                     RQEAEKAVFEDSRTSFSVLWHELGHLVRNEIRGFTPYTDIAGAVNEAYADLFSLMMQQ
                     VVTHGNKPFPDGTKPEHWSRWAIGLDFLKPLKIKLADGTTQERQAAIRQFYAKAFEGH
                     PLMGDDASLHDLPRLDASETEEQSPFFAEECTFDEGGVHIKAASINKAHYLMITKIFE
                     GMAFPAMLLFSTSMGIAKVNGYPTDDLHQLAWLEILAATQLFGAEKAAQVRKVWEDDM
                     KVTGVQLNDPLPVPEQQA"
     gene            1667..2515
                     /locus_tag="VJC18_03105"
     CDS             1667..2515
                     /locus_tag="VJC18_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLD44400.1"
                     /translation="MPVLPKTAKEWYQVRQIRPKLFEVHEPQHVSFFVLKHRSQALFI
                     DSGLGLEPVAAKRLLKKLNIKDFSVLLTHAHCDHIGLSHLAKKIYVNPREWKKHIAQN
                     EIRHLSIYHELLAETLPWPRKYAEHPHTKSFEPTNFIKLNQHISFGPFKLKPLPVPGH
                     TKGSLAFYEPSLNVLFLGDFVYNGIIYINLKDSSLGDYLNSMNRILAFFHKTQPHPLL
                     LPCHNDIPLDPAYLYRVKKVLEKLVSNKIKPRGRWPLKRFFQSSHVYIDQGVRLVVKD
                     DQFPNR"
     gene            2704..3273
                     /locus_tag="VJC18_03110"
     CDS             2704..3273
                     /locus_tag="VJC18_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44401.1"
                     /translation="MGSLTFSCDGQKPVNLNDVLWSDLNNDTIPDANEVTLYDQTLSD
                     EQLRCYTQLLNGTKGSRQIRYTLVPYEKYGQVQNPLDENFFDPSSRVSMERLLNDIAD
                     QLKPGTMHVAFIFAPSDRFPRIRIIGTARNGKEIDSIMSSFYYGCSQAPICKAKVLIG
                     ALSDELFRLKCVEARDICTPPTPPDCGQK"
     gene            3454..4815
                     /locus_tag="VJC18_03115"
     CDS             3454..4815
                     /locus_tag="VJC18_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44402.1"
                     /translation="MLVLCAFSYIMAQNSLFFRESDEKFVTLLKYNKQKQRDIIIVDG
                     IKKIGKSTLIKNRIQHASHVWLDLNKDRVILKEIEASHSFSEFQDILSIRYHFIPNTG
                     LILVIDGAHESKIIGSYIAQMKELWQNQTVILIVSNISLLLRERIKELDPYIVRHTVM
                     PLSFAEFLVATGENYLHDQLANWNIAKPFTDTLHTRALEVLENYLNIGGIPTVVESYL
                     NGQSWNDFFVAYFFDYVAEYKSLHGEERANLFEESLRKTAQSLGMPSKLTTIIPSLKR
                     NYRSVSELFFFLEQLGLVYKIPLKSPAEKKESGKTSKIPPKRYLCDHGIRDHFNQLPL
                     KLLSSLYTNTRTPKGFIKGILENFVLCELKARGLRDAISWRQKINGSEISFIIKTSTN
                     FIPIDIRPSPKTNKKFFSSLQACMSEMALNQSVLFSADKGRVIEFDKQRILQVPFYCV
                     AKL"
     gene            complement(4914..5171)
                     /locus_tag="VJC18_03120"
     CDS             complement(4914..5171)
                     /locus_tag="VJC18_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HLD44403.1"
                     /translation="MAKKKESAFMKPVQPDDVLAAVVGAKPIPRTEVVKKLWEYIKKN
                     KLQDAKNKRNINADDKLKLVFDGKKQVNMFEMTKLVSKHLS"
     gene            complement(5283..>5756)
                     /locus_tag="VJC18_03125"
     CDS             complement(5283..>5756)
                     /locus_tag="VJC18_03125"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HLD44404.1"
                     /translation="KITTGVVDTVQGEAVGWVRAQSSRLKNISIGGNLVPRLIDELPV
                     LALVAACSEGKMLVKDAGELRVKETDRIRAICSQLPKLGVNIEEREDGFLIQGNPAGI
                     RVVDQMTLNTFGDHRLAMMEAVAGLVADKSFVIDDVKNVDTSFPGFFNLLDKIRG"
BASE COUNT         1617 a         1443 c         1170 g         1526 t
ORIGIN      
        1 acgcttcggg gctcactgac aaacccttca ttgagcaggt tctagcagag ggaaaaacac
       61 tcgaagagcg ggttattgcc tgggaatccg aggtgttgcg gattgtggcc tcaaagatta
      121 aataattgtg tttttttttc cctccctctt gcattaattc tcaaattcca ataccctgcg
      181 actgtctaac cccaacgagg aggcaacatg attggcaccg ccagacccgt taccacttta
      241 acatctccag cctttgcatt accgaatgtt tatcttagac acgcggaccc agacgccctg
      301 agggcaatgg gctttcccaa tgaagccccc gatgtgtatc gccaaaaaaa taccgttttt
      361 tttaattatt ggttgcgcga tgcaggacgc ctttccccct tgttccccgg acctgaacga
      421 acgcgtcgtt tcctgcggag attggtagag cggctccctt acggcaatga agctttgcgg
      481 tttttgcggg gacctgctca tcaggacgca tccacagttc ccggcattga gcgggttttg
      541 ctcgacaatg aaggcaaggc cgatcccaac tcagccaaag tattgcgacg ggaaggccag
      601 gccccaaccg gggacaccat taacgacaag ctttttttca atgctggtct agccgtaaaa
      661 caggccaatg gactcatcac tgaagtcaac aagaccttgg ccagaatgta tcctgccctg
      721 acggtagata tcaatatgct acgcggcgcc ttgattttat gcattaacca tgaccccgaa
      781 caggcagata accttggctg gccaaacgcc gcggcggttc ccattgaaaa aggtgtcggg
      841 cttgttttgt ttggattgtt gcgccaagag gccgagaagg ccgtattcga ggattcccga
      901 acctcctttt ctgtgctgtg gcacgaactc gggcatctgg tcagaaacga gatccgcggt
      961 ttcactccct ataccgatat cgcgggggct gttaacgagg cctatgcgga tttattttcg
     1021 ctcatgatgc aacaagtcgt cactcacggc aacaaacctt tccccgacgg caccaaacca
     1081 gaacattggt cccggtgggc cattgggctt gattttctaa aaccgttaaa gatcaagttg
     1141 gccgatggca caacacagga gcgacaagcc gccattcgtc aattttacgc caaggccttc
     1201 gagggtcatc cgctcatggg tgacgatgcg tctcttcatg acctgccccg acttgatgcc
     1261 tctgagacag aggaacaatc cccgttcttt gcggaggaat gcacctttga tgaaggtggc
     1321 gttcacatca aggccgcctc gatcaataag gcacactatc tgatgatcac aaaaattttt
     1381 gagggcatgg ccttccctgc catgcttctt ttcagcacct ccatgggaat tgccaaggtc
     1441 aacgggtatc ctaccgacga tttgcaccaa ctggcttggc tcgaaattct cgcggccaca
     1501 caattatttg gtgcagaaaa ggccgcgcag gtccgcaagg tgtgggaaga tgacatgaag
     1561 gtgactggcg tgcaactgaa tgatcccctg ccagttccag aacaacaggc ctaattgatt
     1621 tcttcacttt gacaaaggcg aaccatttca cttaatagat gcgtctatgc ctgttttacc
     1681 caaaacagca aaagaatggt atcaagtccg ccagatcagg ccaaagctgt ttgaggttca
     1741 cgaacctcag catgtcagtt tttttgttct caaacatcgc tcccaggccc tgttcattga
     1801 ttctggcctg gggcttgagc ctgttgcggc caagcgactt ctcaaaaaat tgaacatcaa
     1861 ggacttttcg gtcctgctca cgcatgccca ctgcgaccac attggactta gtcatctggc
     1921 caaaaaaatt tatgtgaacc caagggagtg gaaaaaacac attgcacaaa atgaaatcag
     1981 gcacctgtca atataccacg aactattagc cgagacactt ccctggccaa gaaaatatgc
     2041 cgagcaccca cacacaaaat cttttgaacc gacaaatttt atcaaattga atcaacacat
     2101 ttcatttggc ccctttaaat taaaacccct tcccgtcccc ggtcacacca aaggaagctt
     2161 ggccttctat gaaccatctc tcaacgttct gtttttggga gatttcgtat acaacggcat
     2221 tatctacatc aacctcaaag acagctcact cggggattat ctgaactcca tgaatcgaat
     2281 tctggccttt ttccacaaaa cgcaaccaca cccccttttg cttccctgcc acaacgacat
     2341 ccccctggac ccagcctatc tatatcgagt caaaaaggtt ttggaaaagt tggtcagcaa
     2401 taaaatcaag ccccgtggtc gctggccttt gaaacgtttt ttccaatcta gccatgtcta
     2461 catcgaccaa ggcgtgcgcc tggtcgtcaa agacgatcaa tttccaaaca gatagtttct
     2521 gattcgcccc ccccctgtgc cccgttgtga tgacgcgact gaatcatcag gattcatctg
     2581 tttttgaatt tttcatttta ccgacaacct ttttagtcac atcgccgaaa cgaagtataa
     2641 gtaccatcgc aacaagcaca atcgggcgcc tattgtcaga tggtagtaaa aaaaggagcc
     2701 tttatgggta gtctcacttt ttcatgtgac gggcaaaagc cagtcaacct caacgacgtg
     2761 ttatggtcag atctcaataa cgacacaatt cctgacgcta acgaagtgac attatacgac
     2821 caaacattgt ccgatgaaca gctgagatgc tatacccagc tactcaatgg aacaaaaggc
     2881 tcaagacaaa ttcggtacac attagttcct tacgaaaaat atggtcaggt gcaaaatccc
     2941 ttggacgaga atttctttga cccctcctca cgcgtttcca tggaacgatt attaaacgac
     3001 attgctgatc agttaaagcc gggaaccatg cacgttgcct tcatctttgc acctagtgac
     3061 cgatttccaa gaatcaggat cattggaaca gcccgaaacg gtaaagaaat agactcaata
     3121 atgagcagtt tttattatgg ttgttcacag gcgccaatct gcaaagccaa agtgttgatt
     3181 ggggcgctgt cagatgaact ttttcgcctc aaatgtgttg aagcacggga tatttgtacc
     3241 cccccaaccc cacctgattg tggacagaaa tgacaacagc aactcttccg atgtccaatc
     3301 attttgatca taaattcggc ggacagccgt tgaactgccg tttttaggtt gattgagtgc
     3361 cactcaattt tctatcgtcc attatccatt tgctttatta tgcaaatatc gctatattat
     3421 gacttgtgaa tcacacaaac atagctatat ttattgttgg ttttgtgtgc ttttagctat
     3481 attatggcac aaaactcctt attttttaga gagtccgacg aaaagtttgt aacgctatta
     3541 aaatataaca agcaaaaaca aagggatatc ataatagttg atggtatcaa gaaaattgga
     3601 aaatcaaccc taataaaaaa tagaatccaa catgcctcac atgtatggtt agatttgaat
     3661 aaagacaggg taatattaaa agaaattgag gccagtcatt ccttcagcga gttccaagac
     3721 attctaagta ttcgatatca cttcattcca aatacagggc taatccttgt catcgacggg
     3781 gcccatgaat ccaaaatcat tggctcgtac atcgcgcaaa tgaaggaact atggcaaaac
     3841 cagactgtta ttctcatcgt ctcgaatatc tccctgcttc tacgtgagcg catcaaggaa
     3901 ttggatccgt acatcgttcg tcacactgtt atgccattgt cttttgcaga attcctggtc
     3961 gccactggtg aaaactatct gcatgatcaa ttggcaaatt ggaatattgc aaaacctttt
     4021 acagacacac ttcacacacg agcactcgaa gtgttagaga actatctcaa tatcggaggg
     4081 attccaacag tcgtcgaatc ttatttgaat ggacaaagct ggaacgattt ttttgtcgca
     4141 tatttttttg attatgtcgc agaatataag tctctgcatg gagaagaaag ggcgaatttg
     4201 ttcgaagaat ctttgagaaa gacagcccaa tcactgggga tgccaagcaa gctgactaca
     4261 attattcctt cgttaaaaag aaattaccgt tctgtgtctg aattattttt tttcttggaa
     4321 cagcttggtc tcgtctacaa aataccgtta aaatcaccag cagaaaaaaa agagtcagga
     4381 aaaacttcaa aaattccacc aaaaagatat ctctgcgatc atggtattcg tgatcacttt
     4441 aatcaattac cccttaaatt gcttagctca ctctacacga acaccagaac acccaagggt
     4501 ttcatcaaag gtatcttgga aaactttgtg ttatgcgaac ttaaggcccg tggcttgcgt
     4561 gatgccattt cctggcgtca aaaaatcaat ggcagtgaga tcagttttat tattaaaaca
     4621 agcaccaatt ttattcccat cgatatcaga ccaagcccaa aaacaaacaa aaagtttttt
     4681 tccagcctcc aggcctgcat gagcgagatg gccctaaacc agagcgtgtt gtttagtgcg
     4741 gacaaaggaa gggtgatcga atttgataaa caaagaatat tacaagtccc gttctattgt
     4801 gtcgccaaat tataaaaaaa accatcttgt cgatatctgc cagcgcgcat acacacgcca
     4861 acacacacct ccaggatggc tttctgctaa ttctctaaga tgagacaaca acgtcagctc
     4921 agatgttttg aaacgagctt ggtcatctca aacatgttca cctgcttttt gccgtcgaaa
     4981 accagtttta atttatcgtc ggcgttgatg tttcttttat tcttggcatc ctgcaacttg
     5041 ttctttttaa tgtattccca caatttttta accacttcgg ttcggggtat aggctttgcc
     5101 ccaacaactg cggctaaaac atcgtctggt tgaacaggtt tcatgaacgc tgattctttc
     5161 ttttttgcca taatgactct ccttactcgt taattaatat tagtcccgcc atgatccggc
     5221 gaaagctaat gacaatcgcg ctttttaaca agcacaaaaa actcaaaaaa aacactgtct
     5281 cactaacctc tgattttatc tagcaaatta aaaaagcccg gaaaagatgt gtccacattc
     5341 ttcacatcat caatcacaaa tgatttgtcg gcaaccaatc cggccaccgc ctccatcatg
     5401 gcgagacggt ggtcgccaaa cgtgttcagg gtcatttggt ctaccacgcg aattcctgcg
     5461 ggattcccct gaatcaaaaa accatcctct ctctcttcga tgttcacacc aagtttaggc
     5521 aactgcgaac aaatggcccg aatgcgatcg gtttccttga cacgaagctc tccggcatcc
     5581 ttcacaagca tttttccctc agagcaagcc gccaccaggg ccaacacagg aagctcgtct
     5641 atcaaacgcg gcaccagatt gcctccgatg gaaatgtttt tcaatcgtga ggattgcgcg
     5701 cgcacccagc ccacagcttc gccctgcacg gtatccacaa cccccgttgt gatctt
//
LOCUS       DATDUN010000238         1834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_385607, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000238 DATDUN010000000
VERSION     DATDUN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385607"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VJC18_03130"
     CDS             <1..321
                     /locus_tag="VJC18_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLD44405.1"
                     /translation="NRFIAEGDHTNKDAFLIVDGLAEIWSYAPHDRRITVGPGTVVGE
                     MASINHQARKANVSAQGHVTVLRFSEADLKDMMEKYPIIRTYLQRLAHQRIMDENRLP
                     RTAA"
     gene            complement(458..625)
                     /locus_tag="VJC18_03135"
     CDS             complement(458..625)
                     /locus_tag="VJC18_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44406.1"
                     /translation="MSYKFGHVPPLQQLPASEHDLAEGQVAFDFEIILLGTLMSFSKS
                     RDSHFGQAMVF"
     gene            complement(671..958)
                     /locus_tag="VJC18_03140"
     CDS             complement(671..958)
                     /locus_tag="VJC18_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLD44407.1"
                     /translation="MKTYYVYILASKKNGTLYVGVTSNLSKRIFEHKQDLVDGFTKKY
                     GVHSLVYIEQTNDVNDALLRERRLKAWKRQWKIDLIEKSNPNWNDLYEDLF"
     gene            complement(1054..1239)
                     /locus_tag="VJC18_03145"
     CDS             complement(1054..1239)
                     /locus_tag="VJC18_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLD44408.1"
                     /translation="MQYSEVMTTNEAMKYLKITRKTLLKLVKTGELKGAKVGKDYRYL
                     KSEIDKFLKGEKEERLF"
     gene            1669..>1834
                     /locus_tag="VJC18_03150"
     CDS             1669..>1834
                     /locus_tag="VJC18_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HLD44409.1"
                     /translation="MADALGMIETKGFVAMVEASDAMVKAAKVELVGYEKIGGGFVTA
                     IVRGDVAAVKA"
BASE COUNT          507 a          433 c          392 g          502 t
ORIGIN      
        1 aatcgattta tcgccgaggg cgatcacacc aacaaagacg cctttctgat tgtggatggc
       61 ctagcggaga tctggtcata tgcgccccat gaccgtagaa tcacagttgg tcccggaacg
      121 gtagtcggcg aaatggcctc catcaaccat caggcacgca aggcaaatgt cagtgcgcaa
      181 ggccatgtca ccgtccttcg ttttagcgag gccgatctga aggacatgat ggagaaatat
      241 cccatcatcc gaacatacct gcaaagactg gcccatcaaa gaatcatgga cgagaacagg
      301 ctccccagaa cagccgcata acaacgccct gatgttgctc catacaattg cccattgcca
      361 attgcccatt gccaattgta ttgggacgca tgcccttatt cgaatacaaa tgcaaaaaat
      421 gtgacgctgt ttttgaattg attgtctcca cgacgcgtca gaataccatc gcctgtccaa
      481 aatgcgagtc ccttgatttg gaaaaactca tcagtgtgcc aagcaggatg atctcgaaat
      541 caaacgccac ctgtccctct gccaaatcgt gttcagaggc agggagttgt tgtagtggcg
      601 ggacgtgtcc gaatttatag ctcaaagctc aaagctttgt cattcctgcg aaagcaggaa
      661 tccatgtttc ctaaaacaaa tcttcataca aatcgttcca attcgggttc gatttttcaa
      721 tcagatctat tttccattgc ctcttccaag ccttcaatct tctttctcta agcaatgcgt
      781 cgttcacatc atttgtttgt tcaatataaa ccaaagaatg aaccccatat ttttttgtga
      841 acccatccac caggtcttgt ttgtgctcaa atattctttt ggacaaattg ctcgtaacac
      901 cgacatacaa tgtgccattt tttttgctgg caagaatata cacatagtat gtcttcataa
      961 atctatgctc tggttccctc cctgcgaagg cagggaccac gcagggatga cagaaactgg
     1021 atccctgcct tcgcagggat gacaagatac gattcaaaac agccgttcct ctttttcccc
     1081 tttgagaaac ttgtcgatct cgcttttgag gtagcgataa tccttgccca ccttggcgcc
     1141 cttgagctca cccgttttga ccaatttgag cagggtcttg cgggtgattt tgaggtactt
     1201 catggcctcg ttggttgtca tgacttcgga atattgcatg agaagagtgg atcgtggatc
     1261 gatgttcgtg aatcgtctaa aagtacaaac agctggtcat tgcgagattc acaaagcgaa
     1321 tcgaagcaat ccacagtatc atttctggat taccccggta tcccttgggg cacagtatta
     1381 aaattgcctt ttggcaattc caacgcagtt caccctctca atgacgagcc acaatccgca
     1441 atctagaaac tgattatcac aattggcact aatcatcaac cattaaatag catcatatat
     1501 cactaaatat ctcgtgttat tataaaatga ttttgaatgt ggttttcttt aacgataata
     1561 tcattaacta ttgacagtcc ccaaaaaaaa agtctatgcc gaccaaactt ggttccctat
     1621 tgtaatagta ataagggggc ctctaaatcg ttaataaagg agattgatat ggcagacgca
     1681 ttaggaatga tcgaaaccaa aggttttgtc gcaatggtcg aggccagtga cgccatggtg
     1741 aaagccgcca aggttgaact tgttggttac gaaaaaattg gtggtggttt tgtgaccgcg
     1801 attgtcagag gtgatgtggc cgccgtgaaa gccg
//
LOCUS       DATDUN010000239         3250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_509818, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000239 DATDUN010000000
VERSION     DATDUN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509818"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VJC18_03155"
     CDS             <1..342
                     /locus_tag="VJC18_03155"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HLD44410.1"
                     /translation="AKKVWLHWAELRVHGEAGAWKTPTGLIPKHEDLARLFKQFFNED
                     YTKEEYAYQFSFRCDKWLAKLERSINYYQKNVPDCPQKVYALWHSAIENIKQAQKRHG
                     AVIKPGEEGGE"
     gene            complement(479..1630)
                     /locus_tag="VJC18_03160"
     CDS             complement(479..1630)
                     /locus_tag="VJC18_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLD44411.1"
                     /translation="MKKNLLFIICALSLGIAPAVGQAKKSGDFALPVQKEFKAQNVRV
                     LSAGWETALKPGSPSKRYYPETNSPALGKEAVYIGTQAGLFYAVDRKNGKILWTFEND
                     EGIAATAGLHKDQVVFSDLGGQLICLNRSTGALLWKKSFDKELLSKPLIRENKIFVVT
                     GEQTITALSLNDGETIWNHALSNYMKKLTIRGIANIVAENGSLYVGLADGHLYKLGAA
                     DGRVLWDKNLALPLSTFKDIDASVVLSQDSLFVGGYSGAIYRIHKGTGNILWTADVAT
                     GSSALVLNDLVIVADTNGALHALNRNNGEELWMNKLNESVSSAPVVFKDKLFVSTFDN
                     EAFLIDPATGNQIQKLSLGKGSINTPLVTRDHIFVFTNNAKLIALREVK"
     gene            complement(1645..2349)
                     /locus_tag="VJC18_03165"
     CDS             complement(1645..2349)
                     /locus_tag="VJC18_03165"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD44412.1"
                     /translation="MDDKQLEKVLTSKSFLKRNEFAEVVAKTSAYCRENKKTVTWAVT
                     VLVILGLFFPVFNWYRLKTVQDFNQALYQAEKSVQKIDKYKELLNKFEKLPAAQLVRL
                     KLAQALVDHKNDDEAITVLKEGLGKGQDELLNTLLVLKSVGLLKEKGRFEEASSFAAQ
                     NESLIIKPFLGQYKLLQGDLLVLADKKAEARKLYEFIVGQTSGMNKEKAELSGYDPRV
                     IDVAKDKLLVLDLNAL"
     gene            complement(2375..>3250)
                     /gene="hemA"
                     /locus_tag="VJC18_03170"
     CDS             complement(2375..>3250)
                     /gene="hemA"
                     /locus_tag="VJC18_03170"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HLD44413.1"
                     /translation="PLTTKALPKARHHVYCKTNKEAVRHLFEVTSSLDSQVIGENQIT
                     NQVKKAYLTALSSKTTGGFLNKLFERALFVSKRVKTETDISRGNLSLGSAAVMLAKRI
                     FGSLSNASVLLLGLGEIGQQVVRALNGHNTQKTWLMTRSLTKATEQEALGLGIARPLE
                     TLCSALSEADIVITALSETLPELTVGSLEKALTCRKDRPLFMIDLGVPRNVDVTVATL
                     ENVYLYNLDDLRHISEETLKSRTNTLCQAKNIIDEETALFYGSSPTQKGPEPKHGDGL
                     GLLKKFFRLDDESEL"
BASE COUNT          795 a          763 c          825 g          867 t
ORIGIN      
        1 gcaaaaaaag tctggttgca ttgggctgag ttgcgggtgc atggcgaggc gggcgcgtgg
       61 aaaaccccca cgggactgat tcccaagcat gaagatctcg ccaggctgtt caaacagttt
      121 ttcaatgaag attataccaa agaagaatat gcctaccagt tttctttccg gtgtgacaaa
      181 tggcttgcca aactggagcg cagtatcaac tattatcaaa aaaatgttcc cgattgtccc
      241 caaaaggtct atgccctgtg gcactccgcg atcgaaaaca tcaaacaggc acagaaaaga
      301 catggcgccg tgatcaagcc gggagaagag gggggcgaat aacgaatatc gaacaaggaa
      361 tatcggatgt caaagtgatg gtttcttgtc atcccagcag aagccaggat ccaagagaca
      421 cactatgatg gattcctgct ttcgcaggaa tgacagaaat aattacttct tgcccaactc
      481 atttaacttc acgcaaggcg attaattttg cattgtttgt aaaaacaaaa atgtgatcgc
      541 gggtcactag gggggtgttg atcgagccct tgccgagtga aagcttttga atttgattgc
      601 ccgtggcggg gtcgatgagg aaggcctcgt tatcaaaggt agaaacgaaa agcttgtctt
      661 tgaacaccac aggggctgac gacacagatt cgttgagttt gttcatccag agctcttcac
      721 cgttgtttct attgagggca tgaagggccc cgttggtgtc ggcaacaatc accaggtcat
      781 tcaataccag cgcggaggaa ccggtggcga cgtcggctgt ccagaggatg tttcctgtgc
      841 ctttatggat acggtaaatg gcaccagaat acccacccac aaagagagaa tcctgtgaca
      901 agacgacaga ggcgtcgatg tctttaaatg tgctgagcgg caaggccaga tttttgtccc
      961 acaacaccct gccatctgcc gcccccagtt tgtataaatg accatcggcc aagccgacat
     1021 acagggagcc gttttcggca acgatgttgg ctattcccct gatggtgagt tttttcatgt
     1081 aattgctcaa ggcgtgattc caaatggttt cgccgtcatt taatgaaagg gccgtaattg
     1141 tttgctcgcc tgtcacaacg aaaattttat tttcccggat cagcggcttt gagagcagtt
     1201 ctttgtcaaa agattttttc caaaggagag cgccggtaga cctgttcaaa caaatgagct
     1261 ggccgccgag atcactgaaa acaacctggt ccttgtgaag gcccgctgtg gccgcaatgc
     1321 cttcgtcgtt ttcgaaggtc cacaggattt tgccgttttt cctgtccacg gcataaaaca
     1381 agcccgcctg ggtgccaatg taaacggcct cttttccaag tgcgggagag tttgtttcgg
     1441 ggtaatatcg tttggaagga gaaccgggtt ttagggctgt ttcccaacca gcagaaagaa
     1501 ccctcacatt ctgcgctttg aattcctttt gcaccggtaa tgcaaaatca ccagatttct
     1561 tggcctgacc cactgctggc gcgatcccca gggataaagc acagatgata aacaaaagat
     1621 ttttcttcat gatgttagcc tctcttacaa agcattcaaa tcaagaacca ggagcttgtc
     1681 ttttgctaca tcaatgaccc gtgggtcata tcccgaaagc tcagcctttt ctttgttcat
     1741 cccgctggtc tgccccacaa tgaactcata aagtttcctg gcctccgctt ttttatcggc
     1801 caaaaccaaa aggtctcctt gcaggagctt gtattgaccc aaaaatggtt tgatgatcag
     1861 gctttcgttt tgtgcagcga aagaggaggc ttcttcgaaa cggccttttt ccttaagcaa
     1921 ccccacggat ttgagcacaa gcagtgtgtt gaggagttcg tcctggccct tgccaagacc
     1981 ctctttgaga acagtgatgg cttcatcatc atttttgtgg tccaccaagg cttgggccaa
     2041 tttgagcctg accaactgtg ctgcgggaag tttttcgaac ttgttgagga gctctttgta
     2101 tttatcgatc ttttggacac tcttttcggc ttgatagagg gcttgattaa agtcctgcac
     2161 ggtttttagt cggtaccaat tgaaaacagg aaagaacaag ccaagaataa ccaaaacggt
     2221 gactgcccac gtgacggttt ttttgttttc gcggcagtag gcagaggttt tggcaacaac
     2281 ttcggcaaac tcattgcgct ttaaaaaaga cttgctggtg agaacttttt ctaactgttt
     2341 gtcatccatg gaagcatctc ctttttgagg tcccttatag ttcggattcg tcatcaagac
     2401 gaaaaaattt ctttaacaga cccaagccgt ctccatgctt gggttccggc cctttctgtg
     2461 ttggggagga tccatagaac agcgcggtct cttcatcaat gatatttttt gcctgacaca
     2521 atgtattggt tctcgatttt agcgtttctt cggagatgtg gcgcaggtca tcgaggttat
     2581 acaaatagac attttcaagt gtggcgaccg tcacatctac gttgcggggg acccccaggt
     2641 cgatcatgaa aagagggcga tctttacgac aggttagggc tttttcgagg gatcccaccg
     2701 tcagctctgg cagggtctcg ctgagggccg taatcacgat gtcggcttcg ctcagtgctg
     2761 agcacagggt ttcaagagga cgggcgatgc ccaacccgag ggcttcctgc tccgtggcct
     2821 tagtcaggga acgggtcatg agccaggttt tttgcgtgtt gtgcccgttt aaggcgcgga
     2881 ccacttgttg cccgatttct cccaagccca gaagcaggac cgaagcgttg gataaggacc
     2941 cgaaaattcg cttggccagc atcaccgcgg ctgatcccaa ggacagattg ccccgactga
     3001 tgtcagtttc tgttttgact cttttggaaa caaataaggc gcgttcaaat aacttgttga
     3061 gaaagccgcc cgttgttttg gacgacaacg cagtcagata ggcttttttg acctggttcg
     3121 tgatctggtt ttcgccgatg acctgtgagt cgaggcttga ggtcacttca aaaaggtgac
     3181 gaacagcctc cttgttcgtt ttgcaataca cgtggtgccg tgctttagga agggcctttg
     3241 tggtgagcgg
//
LOCUS       DATDUN010000240         1008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_137149, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000240 DATDUN010000000
VERSION     DATDUN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137149"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VJC18_03175"
     CDS             <1..228
                     /locus_tag="VJC18_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLD44414.1"
                     /translation="GDKISAAGLTPITSSLVTSPSFEEAQLIIEAKKMYWQDFDPTHF
                     LDPEIEKKYPKKDYHRVYYGEVVAIAKTQSV"
     gene            237..980
                     /locus_tag="VJC18_03180"
     CDS             237..980
                     /locus_tag="VJC18_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44415.1"
                     /translation="MPSFIPKINRTLSLLLVACLILVGWSHFKTEKLPNPDEIQKPLY
                     TAPLQTKTERAPFTQTYDGKTYHISPQYDYELTGLMVSYNDLDEAWFNIYYDNDPYNI
                     KDVCVIWGSNLLKDYYQKVEYKSGSWTCYYRFRSQDVPYFNHEELSNSHLLPATTAIS
                     KTLKRARRGDQIHIEGYLINYSVDDREGQRKSSTTRQDKGQGACEVIYVTEFNILKSQ
                     NAGWRTLNHFAFGLGVLILVLKLYIFLAY"
BASE COUNT          280 a          261 c          208 g          259 t
ORIGIN      
        1 ggcgataaaa tctccgccgc cggcctgacc cccatcactt cttcattggt gacatcgcct
       61 tcgtttgagg aggcccaact catcatcgag gccaaaaaaa tgtactggca ggattttgat
      121 cccacccatt ttttggatcc cgagattgaa aaaaagtacc ccaaaaaaga ttatcacagg
      181 gtgtattatg gtgaggtggt ggccatcgca aaaacacaat ctgtctgaac ccttttatgc
      241 cctctttcat tcccaaaata aaccgcacgt tgagtctcct cttggtggct tgtctgatcc
      301 ttgtgggctg gtcacatttc aaaacagaaa aattaccaaa ccccgatgaa atccaaaaac
      361 ccctctacac ggcaccccta caaacaaaga ccgagcgcgc cccattcacc caaacctatg
      421 atggtaaaac ctaccacatc tcaccccaat acgattatga actgacgggc ctcatggttt
      481 cgtataacga tctggacgag gcatggttca acatttatta cgacaacgac ccgtacaaca
      541 tcaaggatgt gtgcgtgatt tggggaagca atctgctcaa ggattattat cagaaagtcg
      601 aatacaagtc tggcagttgg acctgttatt accgttttcg ttcccaggat gttccctatt
      661 ttaatcacga ggaactttcc aatagtcacc tcttgccagc cacaacagcc atttccaaaa
      721 cgctcaaacg ggcccgtcgt ggcgatcaga tccacatcga ggggtatctg atcaattatt
      781 ctgtggatga tcgcgagggg cagagaaaat ccagcaccac ccggcaggac aagggacaag
      841 gggcttgcga ggtcatctac gtaacggaat tcaatatcct gaaatcccaa aacgccggct
      901 ggcgcacgct aaaccatttt gcgtttggac tcggcgtttt gattctggtg ttaaaactct
      961 atatttttct ggcgtactga ccatgtttca attatttttt taatttat
//
LOCUS       DATDUN010000241         2418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_510009, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000241 DATDUN010000000
VERSION     DATDUN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510009"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VJC18_03185"
     CDS             complement(<1..1006)
                     /locus_tag="VJC18_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLD44416.1"
                     /translation="MKSKTHTIKLISLVVLFFTVSLLIVSDASFGTIKPLNLPNIQRI
                     SRSIFLIQHEYYDPQRVKPLEMFQEGLYEIAKEIPEFLPEFSGNHLTIRLGAKTLDIT
                     LEPFGGLFDVLYPTSQIFDFLHENYHGDVAFDDLEYSFIAGMLYTLDPHSNILTPKIY
                     KEFKTQTEGEYGGLGIVISIKDNELTVVSPMDNSPADKNGIKAEDKILQIDDQITTNM
                     NLDEAADLMRGPPGTKVVLKIKSKNIDPRDVTLVREVINIESVQTKLLTLNNLNFAVV
                     QLKGFQENTFQETVTGLRQIQQKTNNNLQGVVLDLRNNAGGLLDQAILIADLFLKEGD
                     I"
     gene            1161..1772
                     /locus_tag="VJC18_03190"
     CDS             1161..1772
                     /locus_tag="VJC18_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44417.1"
                     /translation="MCKKLFVLLFIFVSLSVVAQATLVKPVNLEQLVALSTYVVRATV
                     TDKQVEHDEYESGGIVTYYTLEVQEWLKGTPPVGQEKQLVIKQLANGEFTSGQKTVRQ
                     NLYFPEYEVGQTYVLFLPVPHHVTGLLAPIGLFQGVYKIQKDEKGKDVVPDYKLRAPL
                     LKKGLVKHVHNAFVTKGLTSAAMGTDYSYETFKTMIQTVSGQL"
     gene            1785..>2418
                     /locus_tag="VJC18_03195"
     CDS             1785..>2418
                     /locus_tag="VJC18_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44418.1"
                     /translation="MKKTLMLSAMFVCLLVGGWAFAGQALYMNSSGTPLVWDASDSDP
                     IIVHPESGACGQFSNVEMLSLLETNLEEWSTVTTVDLAFSVLSGEIDSVDGANYADYL
                     VGVSGLTQAQNEDNVQDGLNPVLFDDDGEIVDQATGENSGRFYILGFASPSGFALNGE
                     GTDYETIVDGQAVFNCYCLEDATGDPAHADCNSILFTTDDLDFTMVHELGH"
BASE COUNT          588 a          504 c          626 g          700 t
ORIGIN      
        1 cgatatctcc ctctttcaag aaaagatcgg cgatcaatat cgcctgatcg agaaggccgc
       61 cggcgttgtt gcgcaaatcc aagacaacgc cctggaggtt gttgtttgtt ttttgttgga
      121 tttgtcgcag gccggtgact gtttcctgaa aagtattttc ttgaaaacct ttcaactgca
      181 ccacagcgaa attcagattg ttcaaagtga gtagtttggt ctgcacactt tcgatattga
      241 tcacctctct gaccagggtg acatcgcgtg ggtcaatatt ttttgatttg attttgagca
      301 ccacctttgt tcctggtggg cctcgcatga ggtctgccgc ttcgtccaga ttcatgttgg
      361 tcgtgatctg gtcgtcgatt tgcaggattt tgtcctctgc cttgatgccg tttttgtcgg
      421 ccgggctgtt gtccatgggg gacacaacag tgagctcatt gtccttgatg ctgatcacga
      481 ttcccaaacc gccgtattca ccttccgttt gagttttgaa ctctttgtag atcttgggtg
      541 tgaggatgtt ggaatgaggg tcgagtgtgt acaacatgcc cgcgatgaaa gaatattcca
      601 ggtcgtcaaa ggcaacgtca ccgtgatagt tttcgtgcaa gaaatcgaaa atttgacttg
      661 tggggtacaa cacgtcgaac aggcctccga aaggctccag cgtgatgtcg agcgtcttgg
      721 cgcccaatct gatcgtgaga tgattgccgc tgaattccgg gagaaattcc ggaatttcct
      781 tggcgatctc atacaggccc tcttgaaaca tttccaaggg tttgactctt tgggggtcat
      841 agtactcgtg ttggatcaaa aaaatactgc gactgattct ctggatgttt ggtaagttca
      901 gcggtttgat cgttccaaaa ctggcgtcgg aaacaatcag taggcttaca gtaaaaaaaa
      961 gaacgaccaa agagatcaat tttatggtgt gagtttttga tttcatgtct gtcatcatca
     1021 taggttaatt gtttgagcga agatgataat atgaatagaa taaatcctca agcaatcaat
     1081 ccccaaaaaa caatggattt ggtgcaggaa actgtgtatt gctcctggac cgatatcatc
     1141 atattagtta tgaggttttt atgtgtaaaa aattatttgt attgctgttt atttttgtca
     1201 gtttgtctgt tgtggcccaa gccactttgg tcaaacctgt taatttggaa caattggttg
     1261 cgttgtctac ctatgttgtg agggccactg tgaccgataa gcaggtggaa cacgatgaat
     1321 acgaatcagg cgggattgtg acgtattaca cactcgaagt tcaagaatgg cttaagggaa
     1381 cacctcccgt tgggcaggaa aagcaacttg tcattaaaca attggccaat ggtgagttca
     1441 cgtcgggcca gaagacagtc aggcaaaact tgtactttcc cgagtacgag gtggggcaga
     1501 cctatgtgtt gtttctgccc gtaccccatc atgtgacagg gcttctggcc ccgatcggat
     1561 tattccaagg ggtttataaa attcaaaaag acgaaaaggg caaagatgtt gtgcccgatt
     1621 ataaattgcg agcccccttg cttaagaagg gtttggtgaa acatgtgcat aatgcctttg
     1681 tgaccaaggg actgacctct gccgccatgg ggactgacta ttcttacgag acttttaaaa
     1741 cgatgattca aactgtttct gggcaactct aagggaactt ctctatgaaa aaaactctca
     1801 tgctgagcgc gatgtttgtt tgcttgcttg tgggcggctg ggcttttgcg gggcaagctc
     1861 tgtacatgaa cagtagtgga accccccttg tctgggatgc ctccgattcc gatcccatca
     1921 ttgtgcatcc tgagagtggc gcgtgtggtc agttctcaaa cgtcgagatg ttgtctttgc
     1981 tcgaaacgaa tctggaagag tggtcaactg tgaccacggt tgatttggcg ttcagcgtat
     2041 tgtctggcga aatcgatagt gttgatggtg ccaattacgc ggattatctg gtgggtgtgt
     2101 cgggcctcac acaggcacaa aacgaggaca atgtgcagga tggtctcaat cctgttcttt
     2161 ttgacgacga tggcgaaatc gtcgatcagg caacaggtga aaacagcgga cgtttttata
     2221 ttctgggttt tgccagccca tcaggttttg cgctgaacgg agagggaacg gattatgaaa
     2281 ccatcgtcga tggccaggct gtgtttaact gctattgtct agaggacgcg accggcgacc
     2341 ctgcgcatgc cgattgtaat tcaatcctgt ttacaacgga tgatctcgat ttcaccatgg
     2401 tgcacgaatt ggggcaca
//
LOCUS       DATDUN010000242         1427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_468474, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000242 DATDUN010000000
VERSION     DATDUN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468474"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VJC18_03200"
     CDS             <1..357
                     /locus_tag="VJC18_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44419.1"
                     /translation="NNRIINVIGDSPLAAQFKIDFRSEPAMTNIAEVAIGCNDKAKVT
                     GVILEDEKAGFHWAYGRSEHLGGTTGPEQFLSPSRICHVDYVYAVGCPIVCKKFEFIF
                     EDGSRKTAIKDGVLLV"
     gene            complement(583..>1427)
                     /locus_tag="VJC18_03205"
     CDS             complement(583..>1427)
                     /locus_tag="VJC18_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HLD44420.1"
                     /translation="DNYFIASFLDCCIVSFLSYLFMTQHSSKKKASFYCDNEIKVYGV
                     NICQAIFHNRPADLIKVYILSEKRKKFAHIIEHCVRHKKGYNLVQNEDLEKLTQSVHH
                     EGICMIVREPKTVTVTDMLAVLKKSSSPVCLLCLDGVQNSHNLGNILRTAAHFGVPFV
                     LVNEPNFITLPASARRVSEGGAEFVRVVFARDLSVVLKNLQEMGFLIVATSSHANKEL
                     NEAALAPKTVFVMGDEVGGISEAVSRQVKTHVSISGTGHVESLNVATATAICVSQWWQ
                     KRKN"
BASE COUNT          366 a          345 c          340 g          376 t
ORIGIN      
        1 aacaaccgca tcatcaatgt catcggtgat agtcctttag ccgcgcaatt caagatcgat
       61 tttcgatccg agcctgccat gaccaatatt gccgaagtcg ccattggctg taacgacaag
      121 gccaaggtca ctggtgtcat cctcgaagac gaaaaagccg gttttcactg ggcctatggc
      181 cgtagtgagc atttgggcgg aaccacgggg ccggaacaat ttttatcccc cagcagaatt
      241 tgccatgtgg attatgttta cgccgtcggg tgccccattg tgtgcaaaaa attcgaattc
      301 atttttgaag acggatcgcg aaagacagcg attaaagatg gggttctgct tgtttgaata
      361 tcgaatatcg aacaaggaat aatgaaggat gcgtcattgc gaggagcgta gcgacgtggc
      421 aagctccatg ttttcaattg ttgcgtgggg atcgccacgt ccttcgactg tactctcttc
      481 aagtccgagc ctgagagctc gttccctcag agtcgaggac aggactcgcg acgacgcttt
      541 actcatttcg acaatccttg ttcgacattc gatattcgat attcagtttt tccgtttctg
      601 ccaccattga ctcacacaaa tggccgtcgc cgttgccacg ttgagacttt ccacatgacc
      661 agtgccactg atggaaacgt gtgttttgac ttggcgtgac accgcctcag aaatgccccc
      721 aacctcatcg cccatcacaa aaactgtttt gggggcaagc gcggcttcgt tcaattcctt
      781 gtttgcatga cttgatgtgg ccacaatcaa aaaacccatt tcctgcaaat tcttcagcac
      841 aaccgacaag tctctggcaa acaccacacg cacaaattcg gccccaccct cactcacccg
      901 ccgggccgat gccggcaatg tgatgaaatt gggctcgttc acaagcacaa acggcacacc
      961 aaaatgcgcc gctgtgcgta aaatattgcc aagattgtgt gagttctgca caccatcgag
     1021 acacaatagg cagaccggag acgaggattt tttgagcact gccagcatgt cggtcaccgt
     1081 gactgtcttg ggttccctga cgatcatgca gatgccttcg tgatggacgg attgcgtcag
     1141 cttttcgaga tcctcgtttt gcacgaggtt gtagcctttt ttgtggcgca cacaatgttc
     1201 gatgatgtgg gcaaactttt ttcttttttc tgacaggatg tagactttga tgagatcggc
     1261 tggtctgttg tgaaaaatgg cttggcaaat gttgacaccg tacaccttga tttcattgtc
     1321 acaatagaaa gaagcttttt ttttggagga gtgttgagtc atgaataaat aggaaagaaa
     1381 tgaaacaatg caacaatcaa gaaatgaagc aatgaaataa ttgtctt
//
LOCUS       DATDUN010000243         1915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_261840, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000243 DATDUN010000000
VERSION     DATDUN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261840"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="tpiA"
                     /locus_tag="VJC18_03210"
     CDS             complement(<1..677)
                     /gene="tpiA"
                     /locus_tag="VJC18_03210"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HLD44421.1"
                     /translation="MTRKRMIAGNWKMNYGVVQSLKFFTEFNRLAKTDEDYPDVVICP
                     PFTSMYPLSIAMQESPLVHLGAQNCHDQDTGAFTGEVSADFLKEMGCDFVIVGHSERR
                     QFFGETDALINNKVKKIFEKLMTPIFCLGETEKERDQAKTFEVLERQLSQGLKGLQTS
                     QVAELVIAYEPVWAIGTGKTATPGQAQEAHEFLRKEIAKSFGTSYAAETRILYGGSVK
                     PDNIQALV"
     gene            complement(674..1855)
                     /locus_tag="VJC18_03215"
     CDS             complement(674..1855)
                     /locus_tag="VJC18_03215"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLD44422.1"
                     /translation="MKYPTIDSIKFKDKTVFVRVDFNVSLDEAGKIRDDSRIKASLPT
                     IHFLREKGAKLVLASHLGRPKGERDVKYTLLPVARRLAELLETEVLFPDDCVGMEVKK
                     LVTDLRDKGVVLLENLRFHKGEEDNDPLFAERLASLADIYVNDAFGTLHRAHASTAGM
                     VKHFKEKAIGRLVEKEVNFLGKLVSEPEKPYVVVLGGAKVSDKIAVIENLLNVADHII
                     IGGGMAYTFLKAQGFDIGKSLFEEQRLSVARRMLERAKVKGIDLILPSDHIIASKFEN
                     EASYQVAKNGDDWNDWMGLDIGPDSIAHFSAVITRARTVFWNGPMGVYEMEHFRDGTN
                     AIAKALAESKAMTVVGGGDSLSAVNKSGFADRITHLSTGGGASLEFLEGKDLPGLKVL
                     L"
BASE COUNT          479 a          571 c          397 g          468 t
ORIGIN      
        1 acaagggctt gtatattatc gggttttacc gaacccccat acaaaattct agtttcggcg
       61 gcatacgagg ttccaaaaga ttttgcaatc tcttttcgca ggaattcatg tgcctcctgt
      121 gcctgccccg gggtggccgt cttgccagtc ccgatcgccc aaacaggctc gtaggcaatg
      181 accaactcgg ccacctgact ggtttgcaga cccttaagac cttgactcag ctgtctttcc
      241 aacacctcaa acgttttggc ctgatctctt tctttttcgg tttctcccaa acaaaaaatc
      301 ggcgtcatca gtttttcgaa aatctttttc accttgttgt tgatgagagc atcagtctcg
      361 ccaaaaaact gccttctttc ggaatgtccc acaatcacaa aatcacaacc catctctttt
      421 aaaaaatccg cgctaacctc tccggtaaag gcccctgtgt cctgatcatg gcaattctgc
      481 gcacccaaat gcaccagagg actctcctgc atggcgatcg aaagagggta catcgaagtg
      541 aacggtggac aaatcaccac atcgggatag tcctcgtccg ttttggccaa ccgattaaac
      601 tctgtaaaga actttaacga ctgaaccaca ccgtaattca tcttccaatt gccggcaatc
      661 atgcgtttgc gcgtcataat aacaccttca atccaggcaa gtccttgcct tcgagaaact
      721 ccagtgaagc ccccccgcca gtcgacaggt gtgtgatcct gtcggcaaaa ccagatttat
      781 tgacagccga caatgaatcc ccgccaccca caacagtcat cgccttcgat tcagccaagg
      841 ccttggctat cgcgttggtg ccatccctga agtgttccat ctcgtaaaca cccatggggc
      901 cattccagaa cactgttctg gcccgcgtaa tcaccgccga aaaatgcgcg atggaatcgg
      961 gaccaatatc gagccccatc cagtcgttcc aatcgtcacc atttttggcc acctgatatg
     1021 aagcctcatt ctcaaacttg ctggcaatga tgtgatctga gggcaaaatc agatctatgc
     1081 ccttgacctt ggccctctct aacatgcgcc tggcaacact cagccgctgt tcttcgaaca
     1141 acgacttgcc aatatcaaag ccctgcgcct tgagaaatgt gtaggccatg ccaccaccaa
     1201 tgatgatgtg atccgccacg ttcaggagat tctcgatcac agcgatttta tcggaaacct
     1261 tggcccctcc caaaacaacc acataaggtt tttcgggttc tgagaccagc ttgcccagaa
     1321 aattgacttc tttttccacc aaccggccaa tggccttttc cttgaaatgc ttgaccatcc
     1381 ccgccgtcga ggcatgcgcg cggtgcaggg ttccgaaggc atcgttcacg tagatatcgg
     1441 ccaaactcgc caacctctcg gcaaacaggg ggtcattgtc ttcttccccc ttgtgaaaac
     1501 ggaggttctc cagcaacacc acacccttgt cccgcaaatc ggtgaccagc tttttgacct
     1561 ccatccccac gcaatcatcc ggaaacagga cttcggtttc caacagctct gccaaacgcc
     1621 tggccacggg taaaagcgtg tacttgacat ctcgctcacc cttgggtctc cccaaatgtg
     1681 acgccagaac aagcttggcc cccttctcgc gtaaaaaatg aatcgtgggc agagaggcct
     1741 tgattcgcga atcatcacgg atcttgcccg cttcatcaag gctcacgtta aaatccacac
     1801 ggacaaagac ggttttgtct ttaaatttga ttgaatcgat tgtggggtat ttcataagca
     1861 catagatcgt ggatcgtgga tcgtgaatcg caccagcaca aacaagccca ctcaa
//
LOCUS       DATDUN010000244         4817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_510189, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000244 DATDUN010000000
VERSION     DATDUN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510189"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VJC18_03220"
     CDS             <1..363
                     /locus_tag="VJC18_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLD44423.1"
                     /translation="GTLFSGLLLLVGLGSGALTLTGIPYYYFLGGCIGFLVVAGNNFA
                     VPHLGTMLTTLIIVACQLVTSALMDHFGILGGETIPLSGLRIAGIVVIFLGAVLVFDN
                     KSPQHHEGTEGFCGRGRN"
     gene            complement(345..417)
                     /locus_tag="VJC18_03225"
     tRNA            complement(345..417)
                     /locus_tag="VJC18_03225"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Phe,seq:gaa)
     gene            708..1118
                     /locus_tag="VJC18_03230"
     CDS             708..1118
                     /locus_tag="VJC18_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44424.1"
                     /translation="MKYISFTIIVLSIVLLSACSNKPCSGKGMSIDTRPLTDQWNKMV
                     PIPSGGELCYSAMLEGDESTRQIEYVGLDPRKTYDLWEKNLVADGWELTSNNDLSSKY
                     EITATKIPAAIKVIISKAVTNKGWCTVTFKPTGT"
     gene            1441..2235
                     /locus_tag="VJC18_03235"
     CDS             1441..2235
                     /locus_tag="VJC18_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44425.1"
                     /translation="MPHQKKILLSVLGLCLFFSQHVSADDSILLIGRLTQQSYCQEKK
                     WVDPHFEIGFVPIVLDTKTDVSAFTQKMVIATGTIDQSLPLKKLFIGSACDEEAQSRS
                     DWVWGKGGSVRVLRAVPAWMRPKLKWNEFETSAIRVASINPFTGLQVRLEGTVVVVAF
                     TNLTGRELKNLSLKAHYEGCYGKPMTAEQETIFPVVAAEKKVTTTFPQIYVNPEKLST
                     PLGDRSEHVISSLQIVSENKDVWFDFDAHAKSDLGLAVECLNDGQK"
     gene            complement(2249..3388)
                     /locus_tag="VJC18_03240"
     CDS             complement(2249..3388)
                     /locus_tag="VJC18_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44426.1"
                     /translation="MVAVNGMVRPLVIGQSFQPSPVVAVSSTTQCDQERPVVVTTSAG
                     YPVDQTAFTGLLKNVFIRDKKRRQRPDVLATPDIKIPLARPIDVKPNEVKRACAEVSR
                     FRSVLAGHVPRDLLRYTEVLSGLSALMDIFPDQEPSDFFTALSATLFSHQQYHGIIDV
                     VFACAKRGCSQSMLDPMAEAKYSGIDVSHARPTDEMKMASTALEHVFNLILHYVNYGK
                     KIESSLNPLLYARCDIFTDFDKKELLCRLETWKDKTPQMTGAQKRRLGPIMTFLRDDL
                     LSRSELYKDCLFGQIQSQRALERLLGIILFEVDMAEAMSSDRERMTVGLDVVGLLTES
                     RLTLPYHGLLLHELDRRLRRGLGGVALPDDVIAGRMKLIEAFVPE"
     gene            3777..4100
                     /locus_tag="VJC18_03245"
     CDS             3777..4100
                     /locus_tag="VJC18_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44427.1"
                     /translation="MSDSTINCTNITSQRFSTQALADSAAEQCTTEATPLESYQKQTE
                     VLPECFGGARCGYYRPAIYAVRIHQPAPVVKKTTTAQATAPVKPAVPTISDPDREVHS
                     WSSIR"
     gene            complement(4298..>4817)
                     /locus_tag="VJC18_03250"
     CDS             complement(4298..>4817)
                     /locus_tag="VJC18_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44428.1"
                     /translation="RVSTFTDPLDEDTDDVAESVTETVDLDATLVRTIDAAEEHNVDV
                     TTMDDSFAALDEDGEAITVTVTKPEALITFDNDTHRMASRTVLAGNLVVDHNLANIRV
                     VFGVGDAGLTHVAGFCDPYEGEMNFTAYTIEEDDTIGAVVGTGSITYANGTVQSSVFE
                     GTTFEITTRPCD"
BASE COUNT         1263 a         1197 c         1125 g         1232 t
ORIGIN      
        1 ggcacattgt tctctgggct gttgctcctc gttggtttgg gttcgggggc actcactctt
       61 accggcatcc cctactatta ttttctgggc ggctgtattg ggtttcttgt ggtggccggc
      121 aataattttg ctgtgcctca tttgggtacc atgctcacca cgctcattat tgtggcctgc
      181 caattggtca cttcggcgct aatggaccat tttggcatct tggggggaga gaccatccca
      241 ctctctggcc tgcgcattgc gggcattgtg gtcatttttc ttggcgcggt gttggtgttt
      301 gataacaaaa gcccccagca ccatgaaggt actgagggct tttgtgggcg ggggcggaat
      361 tgaaccgtcg acacgaggat tttcagtcct ctgctctacc gactgagcta cccgcccaac
      421 cacgaagatg cgatgaaatc gaaccttcga ggaaaatcga gagaggccta tacgtaaccg
      481 gttatcggct gtcaatcgca taatgtaccc ataacacatc taagatgttt ttaattagtc
      541 acccctgcaa aacttcattt cttctgattc aaacagtcat ctgtgacaga tcaaccagcc
      601 aaaacccaca aatattcact gacggatgac gacgtattca ataaatatct tgccaaagca
      661 aacccctgtg catatactct gtcttttttt tataaaagga acacatgatg aaatacattt
      721 ctttcactat tatcgttttg agtattgttc tgctatcggc ctgcagcaac aaaccctgct
      781 ccggcaaagg catgtcgatc gacactcgcc cgctgaccga tcaatggaat aaaatggttc
      841 ccatcccgtc agggggtgaa ttgtgctatt ccgcaatgct ggaaggcgat gagagtacgc
      901 ggcagattga atacgtgggt cttgatccac gtaaaacata tgatctctgg gaaaaaaatc
      961 ttgtggctga cggctgggaa ctcacttcca acaacgatct ctcatcaaaa tacgaaatca
     1021 cggcaacaaa aatccctgcc gccatcaaag tcataatcag caaagccgtc accaacaagg
     1081 gctggtgcac cgtaactttc aagccaacag ggacttagag cctagatgaa agtgttaagc
     1141 tatactcgac gacataagtt cgagacgctt ttgaggcaag gagtgaaatg aggagcccgc
     1201 ggagcgtaca cgcaagtacg tgagcaggca ccgcaatgaa cgacacagca tcaaaagatg
     1261 tatcggactt atgtcgtcga gtataaaacc cacaccacag gcattcacac cttcggagct
     1321 gtcggccttg cccccttgat tggagagatc aaaactccaa attgattttg agtttttccc
     1381 aaatgtgcct atatccgcac aagaagctgg tgacattgcc cagaagagga gaacaaacca
     1441 atgcctcatc aaaaaaaaat attattgtct gttttaggcc tgtgtctctt tttttctcaa
     1501 cacgtcagtg cagatgattc cattttgctc atcggccgcc tcacacaaca atcctattgc
     1561 caggaaaaaa aatgggtcga tccacatttt gagattggat tcgtacccat cgtgttggac
     1621 acaaaaacag atgtgtctgc cttcacgcag aaaatggtca ttgccacggg gacgattgat
     1681 caatcactgc ccctcaaaaa attattcatc ggttcagcgt gtgatgaaga agcgcagagt
     1741 cgcagcgatt gggtgtgggg caagggcggg agcgtccgcg tcctgcgcgc tgtgccagcg
     1801 tggatgcgac caaagcttaa gtggaatgaa tttgagacat ccgcaattcg tgtggcatcg
     1861 atcaaccctt ttacgggatt gcaggtcagg cttgagggga cagtcgtggt ggtcgctttc
     1921 acgaatttaa cggggaggga gttaaaaaac ctttcactca aagcccacta tgagggctgt
     1981 tatgggaagc ccatgaccgc tgagcaggaa acaatatttc ctgtggtcgc ggccgaaaaa
     2041 aaggtaacga ccacgtttcc ccagatctat gtcaatccgg aaaagttgag cactccgctt
     2101 ggggatcgca gtgaacacgt catttccagc cttcagattg tgagcgagaa taaagatgtg
     2161 tggtttgatt ttgatgcaca cgcgaaatcg gacttggggc tggcggtgga atgtttgaac
     2221 gatgggcaga aataatggaa aaacgtgatt actctggcac aaaggcctca atcaatttca
     2281 tgcgacctgc gatcacatca tcgggcaaag ccacaccacc cagcccgcgt cgcaaacgcc
     2341 tatccaactc gtgcaaaagt aacccgtggt acggcaaggt gaggcggctc tcagtgagta
     2401 gtcccaccac atcaagcccc acagtcatgc gttcccggtc cgatgacatt gcctcagcca
     2461 tatccacttc aaataagata atccccaaca atctctccag cgcacgttgc gactgtatct
     2521 gaccaaaaag acaatccttg taaagctcac tacgactgag tagatcatcc cttaaaaagg
     2581 tcatgatggg ccccagcctt cttttttgtg cacccgtcat ttgaggagtt ttgtctttcc
     2641 aggtttccaa tctgcataac aattcttttt tatcaaagtc cgtaaagata tcacaacgtg
     2701 catagaggag gggattcaac gaggattcaa tttttttgcc gtaattaacg tagtgcagaa
     2761 tcagattgaa aacatgctcc aatgctgtgc tggccatttt catttcatca gtgggcctgg
     2821 cgtgagagac atcgattcct gagtatttgg cctcggccat gggatccagc atggattggg
     2881 aacacccgcg ttttgcgcac gcaaaaacca catcgatgat accatgatac tgttgatggg
     2941 aaaataatgt ggcagacaag gctgtgaaaa aatcagacgg ttcttgatcg ggaaaaatat
     3001 ccatcaatgc ggataatcca gaaaggactt cagtgtacct caataagtca cgtggtacgt
     3061 gacccgccaa cacagaacga aaacgactca cctctgcaca ggccctcttc acctcatttg
     3121 gtttgacatc aattggccgg gccaatggta ttttaatatc gggcgttgca agaacatctg
     3181 gtctttggcg tcttttttta tcacgaataa aaacgttctt taatagccct gtgaatgccg
     3241 tctgatccac aggatagcca gcagaggtcg tgaccacgac aggcctttct tggtcgcact
     3301 gagtcgttga tgacacagca acgacaggac tcggttgaaa actctggcca ataacaaggg
     3361 gtcggaccat tccattgact gcaaccatat cagctcctta atgcccctat atcgacctca
     3421 caggttaaaa gttgctcaga aatttaaaaa aaacagcctt ttttgtcttt tttttgactt
     3481 ttttaccgtt tctttgttga ctcactgcat ccttttctat aagcaggttg tttgcgcgta
     3541 atataaatat gcgcatttat gccgctttga caatttcttc aaacaaaaca tgaggtacgt
     3601 gagtttatag gcgttagggt tgccgtcatt aacaatgcga acaacctact tttaaaaaac
     3661 ccagcactgt tgacaacatc aactagtttg caacaaacaa tcattctaaa ccaatgccct
     3721 catgatgatg agagccggtc tagtatccgg tagtttgtga ccattgggaa caagccatgt
     3781 cggactcaac tatcaactgt acaaatatca ccagccaacg tttttctaca caagccttgg
     3841 ctgattctgc cgctgagcaa tgcacaacgg aagccacccc tctcgagtcg tatcaaaaac
     3901 aaaccgaggt tttgcctgag tgttttggtg gggcacgttg tggttattat cgtcctgcca
     3961 tttatgccgt tcgcattcat cagccagccc ctgttgtaaa aaaaacaacc acagcacaag
     4021 ccactgcacc tgtaaaaccc gctgtaccca caatttccga ccctgatcgc gaagttcata
     4081 gttggtcgag cattcggtag ctcgcaagca ctgcggtacc ggtgtggtcc ttgggtcact
     4141 atactcagcg ccataagtcc aatacatctt ttgatgctgt gtcgttcatt gcggcgcctg
     4201 ctcacgtact tgcgtgtacg ctccgcgggc tcctcatatc actccttgcc tcaaaagcgt
     4261 ctcgaactta tggcgctgag tatatctcct gttttgttca gtcgcagggt ctggttgtga
     4321 tttcgaaggt tgtcccctca aaaacagaac tctgcactgt gccattggca taggtgattg
     4381 agccggtgcc aaccacagcg ccgatggtgt catcttcctc aatggtgtaa gcggtgaaat
     4441 tcatctctcc ttcataggga tcacagaaac cggcgacatg ggtcaaaccg gcatctccca
     4501 caccaaagac cacacgaata tttgccagat tgtggtcaac caccagattg cccgcgagga
     4561 cggtgcggct ggccatgcgg tgcgtgtcgt tatcgaaagt gatcagggcc tccggtttgg
     4621 tgacggtgac ggtgatggcc tcgccgtctt catccaaagc cgcaaaagag tcgtccatcg
     4681 tagtgacatc cacattgtgt tcttcggcag catcaatggt gcggactagg gtggcatcga
     4741 gatcgacggt ctctgttacg gattcagcca cgtcgtcagt gtcctcatca aggggatccg
     4801 taaatgtgga gactcgg
//
LOCUS       DATDUN010000245         1127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_346445, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000245 DATDUN010000000
VERSION     DATDUN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346445"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..141
                     /locus_tag="VJC18_03255"
     CDS             4..141
                     /locus_tag="VJC18_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44429.1"
                     /translation="MSTSNFHTCALLLAGTMKCWGNNSYGQLGNGTTVDSFVPVGVVE
                     E"
     gene            complement(267..>1127)
                     /locus_tag="VJC18_03260"
     CDS             complement(267..>1127)
                     /locus_tag="VJC18_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44430.1"
                     /translation="KRTNRELLEIFFTVGGVPQYLDKFNLDRSPQLNIDQLFFTPDGY
                     FLQEFDRLFASHFGKNPVYKKILTHLAKEKFSSRDDLLHALQMTEGGRLSDYLEELQL
                     ADFVEPYSPVNKPASSVLIRYRIADPFLLFYFRFVYPNIRKIKTQRKHVGRKAFVSDQ
                     AYAIWQGLAFEYFCDRNHELIAEKLGFSAVQYEHGAWFKKRDLLTGGSQVDLLFLRSD
                     RVVTLCEIKFQSEPIGKNIIEAVQNKIRLFPNPRQLTIETVLITATPATPELVNEGFF
                     RSIITVNDLI"
BASE COUNT          305 a          234 c          281 g          307 t
ORIGIN      
        1 tccatatcaa caagcaattt tcatacatgt gctttactat tggctggaac aatgaagtgt
       61 tggggtaata atagttatgg tcaattgggt aatggtacaa ccgttgattc atttgttcca
      121 gttggtgttg ttgaagagta aataaattag gaggacagac aagcaacggt atcaagtctt
      181 gatgccccac ctgtcagtgg caagacagca caagttgcca agacctcgga agagaatacc
      241 ttccattggg gtttttagag gtgcccttaa atcaaatcat tcaccgtgat gatggagcgg
      301 aaaaaccctt cgttgaccag ctctggcgtt gccggtgttg ccgttatcag aactgtttca
      361 atcgtcagtt ggcggggatt ggggaaaagt cgtattttgt tttgcacagc ctcaatgatg
      421 tttttgccga tgggctctga ttgaaacttg atttcgcata acgtgacaac acgatcggac
      481 cgaaggaaca gtaaatcaac ctgcgagcca ccggtcagca ggtctcgctt tttaaaccat
      541 gcaccgtgtt cgtattgcac ggcgctgaac cccagctttt ctgcaatcag ttcgtgattg
      601 cgatcacaaa aatattcaaa ggccagccct tgccagatgg cgtaggcctg gtcagaaacg
      661 aacgctttgc gccccacatg ttttctctgt gttttgattt ttctgatgtt cggatacacg
      721 aagcgaaagt aaaacaaaag aaagggatca gcgatgcgat agcggatcaa aactgacgac
      781 gcaggtttat tgacagggct gtagggttca acaaaatctg ccaattgcaa ttcttccaga
      841 tagtcgctga gccttccccc ttctgtcatt tgcagagcat gcaggaggtc gtctcgtgag
      901 gaaaactttt ctttggccag atgcgtaaga atttttttat acacggggtt cttgccgaag
      961 tgactggcaa aaaggcgatc aaattcctgc agaaaatatc catcaggtgt aaagaacagc
     1021 tggtcgatgt taagctgtgg agaacgatcg agattgaatt tgtctagata ctgcgggacg
     1081 ccaccaacgg tgaaaaaaat ttcgaggagt tccctgttgg tgcgttt
//
LOCUS       DATDUN010000246         2664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_96654, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000246 DATDUN010000000
VERSION     DATDUN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96654"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VJC18_03265"
     CDS             <1..484
                     /locus_tag="VJC18_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44431.1"
                     /translation="QEVGQVIRETATQNPQMLAKLANDLETFKDDAAKRRKRRFFWSR
                     KLIREFSDDDLGVILSLCDAYILRISELIKERYDQTKDGMNVVFDESGQLLLNGMNIH
                     SLIENCRENPTEKSLVFLKGIRSRLGHVLQNKINSRNYDRIQEVVKGLLGDIEKILID
                     "
     gene            591..>2664
                     /locus_tag="VJC18_03270"
     CDS             591..>2664
                     /locus_tag="VJC18_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HLD44432.1"
                     /translation="MPLCRPVRNSLATTLDTINTYTLHWPLLGLFFLQTLLCLHLKTD
                     HFVWPLMYSLSLLYLLCCYARARKYLSLLLVLVLPLVSITCHQLTRKPVAENLYNKKQ
                     TYVATIVKGDQYTNDQSSFVIDLEHQLTPKTNNISGRIMLTIGEACPLTNKSRVLFTA
                     KIKKPTVYLNPGAFNYQQYLSQKNIWGKAFMQHCGDIIPVFLNPHSLRDKLRTRIRAP
                     FQAISLANANLFSPLTIGTPTISWENKRIIQQAGVSHLFVISGSHFGAMCAIIYCLLT
                     LIANRFPALFNICPRQKIAALGTGLFVIFYLWISIPSPSIMRAGLTALLYFAAILLDR
                     QKNLLYLLLLAATANLLIAPHDLFEISFQLSYLCVLILIVIYPRLLTLLRLTDWIRKR
                     SGGIKLFVHTGLVSLLINLTLMPLIIYFFGTAPTQGFINNLWAIPYFDFVVIPLTLLA
                     MLLQMILPHWTPVFLRLWDHCLTLFFKILAVVDSWQLPVINAPEPHAITIICFYCFVF
                     GIFVAGKKRGPVIPLVLCVFSLWFTFESKQMTSDLKITQIDVGQGDAVLVQTRKKNIL
                     IDTGGHRFMDIGTRVLVPYFRHLWLKRLDIVVITHADLDHFGGLSTLIDNVAIGEIWI
                     TRNTAQDKSFAQIFDKMFLKNIPLKIVSQGDASLIDQNTSLAVLAPPAVHRTPKRNKN
                     ELSLVLRLK"
BASE COUNT          667 a          755 c          537 g          705 t
ORIGIN      
        1 tcaggaggtg ggacaggtga tcagggaaac cgccacccag aatccacaaa tgttggccaa
       61 gctggccaat gatctcgaaa cgttcaagga cgatgccgcc aagagacgaa aacgccgctt
      121 tttttggagt cgcaaactga tcagggaatt cagtgacgat gatttgggcg tgatcttgtc
      181 tctctgtgat gcctacatct tgaggatcag cgagctcatc aaggagcgtt acgatcaaac
      241 aaaggacggg atgaatgtgg tgttcgatga gagcggacag ctcttgctca atggcatgaa
      301 cattcacagc ctgatcgaaa attgccgcga gaatcctact gaaaaatcgc tggtttttct
      361 caaaggcatc agatcgcgtt tgggccacgt tttgcaaaac aaaatcaaca gtcgcaacta
      421 cgaccgcatt caagaggtcg tcaaaggcct cctgggtgat atcgaaaaga ttctcataga
      481 ctaaccgtcc cactatgcgt ctcaccactg agacgctccc aaaaatcata caaaaaacac
      541 caatgatttc gaactatcct tgtggcacaa ttcttgaatg agtattcgac atgcctttat
      601 gtcgtcccgt tcgaaactct ctcgccacaa cccttgacac aatcaacacg tacacgttgc
      661 attggccact cttgggcctg ttctttctgc aaacactgct ttgtctccac ctcaaaacgg
      721 atcacttcgt ctggcctctt atgtacagcc tctcgttgct ctatctgttg tgttgctatg
      781 cccgtgccag aaaatacctt tcgctccttc tcgtcttggt cctcccgtta gtaagcatca
      841 cctgtcacca actcacacga aaacctgtcg ccgaaaatct ctacaataaa aaacaaacct
      901 atgttgccac catcgttaaa ggagaccaat acacaaatga tcagtccagc tttgtgattg
      961 atcttgagca ccaactcaca ccaaaaacaa acaacatctc tggacgcatc atgttgacca
     1021 ttggagaggc ctgccccctc accaacaagt cgcgcgtcct ttttaccgcc aaaatcaaaa
     1081 aaccaacggt gtacctcaat cccggggcat tcaattatca acagtatctg agtcaaaaaa
     1141 acatctgggg aaaggccttt atgcagcact gtggggacat cataccagtc tttctcaacc
     1201 ctcatagcct tcgcgacaag cttagaacgc gtattcgcgc cccttttcaa gctatctctt
     1261 tggcaaacgc caatcttttc tccccactga cgatcggcac accaacgatc agttgggaaa
     1321 acaaaagaat cattcagcaa gccggcgtgt cgcacctttt tgtcatttcg ggctcacatt
     1381 ttggcgcgat gtgcgccatc atctactgcc tcttgacgct cattgccaat agattccccg
     1441 ctctcttcaa catctgtcca cgtcagaaaa tcgcggcctt ggggacaggc ctcttcgtca
     1501 ttttttacct gtggatttct atcccgtctc cctccatcat gcgcgcgggt ttgacagcac
     1561 tgctttattt tgcggccatc ctgctcgatc gccaaaaaaa tctcttgtat ctgctactac
     1621 tcgccgccac cgcaaacctc ctcatcgccc cgcatgacct ttttgaaata tcttttcagc
     1681 tctcttatct gtgtgtgctc atcctgatcg tcatctaccc ccgtctactg acccttctgc
     1741 gcttgaccga ctggatcaga aaacgttctg gtggcataaa actctttgta cacacaggac
     1801 tcgtgtcgct attgatcaac ctgaccctca tgccactcat catttatttt tttgggactg
     1861 cccccaccca agggttcatc aacaatctct gggcgatccc ctattttgat ttcgtcgtca
     1921 tcccattgac cttgctggcc atgttgctcc aaatgatttt gccccactgg acaccggtgt
     1981 ttctccgcct gtgggatcac tgcctgaccc tgttttttaa aattttagct gtggtggatt
     2041 cctggcagct ccctgttatc aatgcgcccg agccccacgc cataaccatc atctgctttt
     2101 attgttttgt ttttgggata tttgttgccg gcaaaaaaag agggcccgtg attcctctgg
     2161 tgttgtgtgt tttttctttg tggttcacct ttgaatccaa acaaatgacc agcgacctca
     2221 aaatcacgca aatcgatgtg ggacaaggtg atgccgttct ggtccaaacc cgaaaaaaaa
     2281 acatcctcat cgataccgga gggcaccgtt tcatggatat tggtacgcgg gtgctcgttc
     2341 cctatttccg gcacttgtgg ctgaaacgac tggacatcgt ggtgatcaca cacgccgatc
     2401 tggatcattt tggaggatta tccaccctca tcgacaatgt ggccattggc gaaatctgga
     2461 tcacgcgcaa cacggcacaa gacaagtcct ttgcacaaat ttttgacaaa atgtttttaa
     2521 aaaacatccc gttgaaaatc gtcagccagg gtgatgcctc gctgatcgat caaaacacct
     2581 cattggccgt gttggcccca cccgctgttc accgcacacc caagagaaac aaaaacgaac
     2641 tctccctggt cctgcgactc aaac
//
LOCUS       DATDUN010000247         1463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_386195, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000247 DATDUN010000000
VERSION     DATDUN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386195"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VJC18_03275"
     CDS             complement(<1..953)
                     /locus_tag="VJC18_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLD44433.1"
                     /translation="MKKVLMGNHAASWAVQLARVKMIAAYPITPQTQIVEELSEMCAA
                     GALDAKFLKVESEHSALASCIAASSTGVRAFTATSSQGLLLMHELVHWAAGARLPLVM
                     VNVNRAVGPGWNIWADQTDSLSQRDTGWIQYYVEDNQEVLDTVLMAYQLAEEVNLPVM
                     VCYDAFFLSHTYEPVDIPDQQTVDAWLKPFHRENILDTKDPHAFNALVMPDVYMELRH
                     KMQKAHEETIRVDKKVAASFHKTFGRSYPAVEAIHCDDADVVLVTSSSTVSPSRLVIE
                     KLRKQGIKIGLVKTRLFRPFPTDDVCAVLKGRQKAIVIDRN"
     gene            complement(950..>1463)
                     /locus_tag="VJC18_03280"
     CDS             complement(950..>1463)
                     /locus_tag="VJC18_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLD44434.1"
                     /translation="NPRTVSVAIGAGKAAAIAIDQFLRKEKGSIKTVIGEFGAHSIMA
                     YAGSGVAHQKARGISRVVSFDDINTNHFEPQPRLMKPVISMLDRQASFNEVSQTIAPE
                     TAFAEAERCFHCGVCTMCDTCYAFCPDVAISFKQMNKWGYDIAYDYCKGCGVCAHECP
                     RAAIVMEDEK"
BASE COUNT          354 a          428 c          332 g          349 t
ORIGIN      
        1 aaattgcgat caatcacaat ggccttctgg cgacctttga ggactgcgca aacatcatcg
       61 gttggaaaag gcctgaacag tctggttttg acaagcccga ttttgatccc ctgttttctt
      121 aatttttcga tcaccagccg cgatgggcta accgtagaac tgctggtcac cagcaccaca
      181 tcagcatcgt cacaatgaat cgcctcaaca gccggataag atcggccaaa ggttttatgg
      241 aaagaggcgg ccactttttt atcgacccga atcgtttctt cgtgcgcctt ctgcatttta
      301 tggcgcaact ccatgtacac atcaggcatc accagcgcat tgaacgcgtg tgggtctttg
      361 gtgtcgagaa tattttcgcg atgaaagggt ttgagccacg catcgacagt ttgctgatca
      421 gggatgtcca ctggctcata agtgtgcgaa agaaaaaatg cgtcataaca aaccatcaca
      481 ggcaaattca cttcttcagc cagctgatac gccatcaaca ccgtatcgag cacttcctga
      541 ttgtcttcca cgtaatactg aatccaaccc gtgtcccttt gcgacaatga atccgtctgg
      601 tctgcccaga tattccaacc cggccccacg gctcggttca cattaaccat gacgagcggc
      661 aacctggctc ccgccgccca gtgcaccagt tcgtgcatga ggagcagtcc ctgcgatgac
      721 gtggccgtaa aagcgcgcac acccgtactg gaggccgcaa tgcaggaggc caacgcggaa
      781 tgttctgatt cgacttttaa aaatttggca tccagcgctc ccgccgcgca catctcggat
      841 aactcttcca caatctgcgt ttgcggggtg atcgggtacg ccgcaatcat cttcacccgg
      901 gccagttgca ccgcccaact cgccgcatga ttgcccatga gaactttttt catttttcgt
      961 cctccatgac aatcgccgcc cgagggcatt cgtgcgcgca cacgccgcaa cctttgcaat
     1021 aatcgtaagc gatgtcataa ccccacttat tcatctgctt gaaactgatc gccacatcgg
     1081 gacaaaaggc ataacacgtg tcacacatcg tgcacacacc acaatgaaaa cagcgttcgg
     1141 cctcagcaaa agccgtttcc ggggcgatcg tttggctcac ttcattaaaa cttgcctgtc
     1201 gatccaacat cgaaatcaca ggtttcatga gccgtggttg tggttcgaaa tgattcgtgt
     1261 tgatgtcatc gaaactcacc acacgtgaaa tccctcgcgc cttttgatgt gccacaccag
     1321 agcctgcata ggccatgata gaatgggcac caaattcacc aatcactgtt ttgatggacc
     1381 ccttttcttt gcgcaaaaac tgatcgatcg cgatggccgc ggctttgcca gccccaatcg
     1441 ccaccgacac tgtccgcggg ttg
//
LOCUS       DATDUN010000248         4742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_53839, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000248 DATDUN010000000
VERSION     DATDUN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53839"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4195)
                     /locus_tag="VJC18_03285"
     CDS             complement(<1..4195)
                     /locus_tag="VJC18_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44435.1"
                     /translation="MSVTLNMPDQTVFDEQDVDAWETWLASQDDSVMQAFFDYASGEV
                     SSSSDFSGVPSQDAFYRALDEYGQKHQLEFIIPSSKRMGGSGLLTSASASSFLRLGAT
                     ETSRQPTYRAQAKDVAFDQIRFATSDTQFCNWLYNGMSGYATVDSNAGLTVCAQDYHK
                     DLMDGVFGGVKLSTTQRDFMAGLFKGQTITLSSEGQITSAVTTDITQPSAVVYGDVDS
                     FFEACHLGAVSAEHEGDTGFGNFVGDRLLDTGTDEEAIEALLRSYQEYPEEWAQFKQQ
                     VGLDFRAQGYSFEEIISREFAGGRELGYISGVIQSLNVGNDYYIGQSFGPMGERLMGL
                     LELQGQINPDDPESVETYNQQFDAIKNQCVKTLGEDYAGVFDLNTEQVARVENLQTLA
                     ALTSSVRTLEQYQHEFPALLNAYDIHPPVDIFCWSNLIGPAGVMMNNVDLATSYFRRS
                     PKTEALGIAFKEYMIQLNEVLSDIRAGQEPSPESTQSLIGKMGVVEREFQIFKTSEEA
                     GREQLISSYEAAETGAYTVVGASIAITMGVYAMPLAIEAGMTTVGVLGTGMLAGTVAG
                     TTAEFSLRSAIDVWRPDVDVTAERLGYNLDEASTNAACAALSLATGGGMTALSQTTKS
                     GIIRWIATNPVSRNILTNEAIAVANAGMRADETFGLTDLLVAGGSGALAGVAQPALCN
                     RALAELVFEVNREGFENIAEIQLEGGKLGAEQIIGAIAAGFTELGGSYHGSQMSVNQQ
                     QEIMLELIRRMQVEAVANGLPFNVDAEAIRRGEFDPQLLATEMARLASQRGETSPQGA
                     EQVSEVVTPTDVEEQTETVGDVASHEVTTVQFDKSAGARSVLIAQLIALRDAVQPGQT
                     YVEVTVAVADVSQYNQTDFREIFEQVFAGTDLGAVRLWIEDGNTGLKARDDVVIDRQK
                     SGVPVFTSQDGQAIIGDMFGVEAQGIFDMIARCDPSVQEKVWKLVARVHDEMTTTRLI
                     NRRGIKQSAYGLVPAVDKSEAKQTLQEFLTAVDEFRTSLSAPVARGSFFRRMTATPAD
                     VDSFIENYPARPADCETQGKMTTFVDKKGQPKDYFVPDWVMQKFDEWMSRQSMDMTSE
                     VHGKFVFEENNGVVTLTDFVPLPSLAQSTEYKRARMSEQAYAAQYGDAYTDCYAAPEF
                     ALVAEAFGYDGVAPETVYAANEMPVHSHYLDSAYQTGPSPNDRKGTPRVEAVRTSQAG
                     LVLYQRGRSDHAPKLVVDKPLLPADQTQAFVSRSSPLPVELSEELSSMGEGSSTASVD
                     YGDLRFYCIGGDYYVARPRAGDDPVMLYDQNGRQVYALYSTKQVMHGEGSRASFVPLD
                     YVPLDEGMTIQLADGTKMVFKKPAFTFGERVRINDPRDTDMYTTQGILNGRVILVSDR
                     GRFMLVPVANVTRVAQENPV"
     gene            4393..>4742
                     /locus_tag="VJC18_03290"
     CDS             4393..>4742
                     /locus_tag="VJC18_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HLD44436.1"
                     /translation="MTDQLSLITALPFILTLLSIAIIPLAAPHFWEHNRNKAVIAGLL
                     ATPILVYFLGHDAHLILKTVEEYFSFIVLLGSLYVIAGGINISGDLKATPKVNACFLG
                     IGAILANLIGTTGA"
BASE COUNT         1127 a         1387 c         1074 g         1154 t
ORIGIN      
        1 tgactggatt ctcctgcgcg acgcgtgtaa catttgcaac aggaaccaac atgaacctgc
       61 ctctatcgct gacaagaatg acacggccgt tcaaaatgcc ctgtgtggta tacatatcgg
      121 tgtcgcgagg atcattgatt ctaacccgtt ccccaaaagt gaaggcaggt tttttaaaaa
      181 ccattttcgt tccatcggca agctggatcg tcatgccctc atccagcggg acataatcaa
      241 ggggaacaaa cgatgccctg ctcccctcac cgtgcatcac ctgcttggtg gaatagaggg
      301 cgtaaacctg ccgaccattt tgatcgtaga gcatcaccgg gtcatcaccc gcccgggggc
      361 gtgccacata ataatcccca ccgatacaat agaatctgag gtcaccataa tccacagatg
      421 cggtggaact gccttctccc atcgacgaca gttcttcgct aagctcgacg gggagagggg
      481 aggaacgaga aacaaaggcc tgtgtttgat cggcgggtag taatggtttg tcaaccacca
      541 gtttcggcgc gtgatcactg cggcccctct ggtacaagac cagacctgcc tgcgaggtac
      601 ggacagcctc aactcgcggg gtgcctttgc gatcgtttgg tgagggtccc gtctggtaag
      661 ctgaatcaag gtaatggcta tgtacgggca tttcgttggc ggcatacacc gtctcgggcg
      721 caactccgtc atatccgaat gcctccgcca caagtgcaaa ttccggtgcc gcataacaat
      781 ctgtgtaggc gtctccatac tgggccgcgt acgcctgctc agacatgcgc gcccgtttat
      841 attcagttga ctgggccaac gacggcaacg gcacaaaatc agtcaaagtc accacaccat
      901 tattttcttc aaaaacaaac ttgccatgca cttcactagt catatccatg ctctgccgtg
      961 acatccattc atcaaatttt tgcatcaccc aatctggcac aaaataatct tttggttgac
     1021 cctttttgtc gacaaaagtg gtcatttttc cctgcgtctc acaatctgct ggacgtgccg
     1081 ggtagttttc aataaacgaa tccacatcag caggtgtcgc cgtcattctc ctgaaaaaag
     1141 aacctcttgc aacaggagca ctcagcgaag tcctgaactc atcaacggct gtcaggaact
     1201 cctgcaatgt ttgttttgct tcggacttgt ccacggcagg aaccaatcca taggcactct
     1261 gtttgattcc ccgtctgtta atcagccgag tcgttgtcat ttcgtcatga acacgggcca
     1321 caagtttcca aactttttcc tgaactgagg gatcacaacg ggcaatcata tcgaaaatgc
     1381 cctgtgcttc cacaccaaac atatcgccaa tgatagcctg accatcttga gacgtaaaca
     1441 ccggcacgcc tgatttctgt ctatcaataa caacatcgtc tcgcgctttc agccccgtat
     1501 tgccatcctc aatccacaaa cgcactgcac ccagatcggt cccggcaaag acctgctcaa
     1561 aaatttctct aaaatctgtt tggttgtatt gactcacatc tgcaacggcc acagtcacct
     1621 ccacgtaggt ttgtccgggc tgaacggcat cgcgtagcgc tatcaactgt gcgatcaaga
     1681 cagaccttgc ccctgctgat ttatcgaact gtacggttgt cacttcatga ctggcaacgt
     1741 cgcctaccgt ttctgtctgt tcctctacat cggttggtgt gaccacttca ctgacctgtt
     1801 ctgctccctg aggagacgtc tctcccctct gcgatgccag ccgtgccatt tcggttgcca
     1861 ataactgcgg gtcaaattcg ccacgccgaa tggcctccgc atccacgttg aacggtagtc
     1921 catttgccac agcctcaacc tgcatccgtc tgatcaactc caacatgatt tcctgttgtt
     1981 gattcacact catctgcgag ccgtggtacg aaccgccgag ctccgtgaat cccgccgcga
     2041 tcgcgccaat gatctgctcc gcccccaatt tgccaccttc cagctgaatc tccgcaatgt
     2101 tctcgaaccc ctcgcgattg acctcgaaga cgagctccgc cagggccctg ttgcacaacg
     2161 caggttgggc cacacccgcc aaggccccgc tcccaccagc caccaaaaga tcggtcaacc
     2221 cgaacgtttc atcagcacgc atgccagcgt tagccacagc aatggcctca ttggtaagga
     2281 tgttgcgact caccgggttg gtcgcgatcc aacggatgat ccccgatttt gtggtttgcg
     2341 aaagggctgt catcccgccc cccgtggcca gactcagagc ggcacaggcc gcgtttgtgg
     2401 acgcctcatc aagattgtat cccagtcttt ccgcggtcac atccacatcg ggacgccaca
     2461 catcgatggc cgatctcagg gaaaattccg ccgttgttcc cgccacggtc ccagccaaca
     2521 tcccggttcc caacaccccg accgtcgtca tgcccgcttc aatggcgagc ggcatggcat
     2581 aaactcccat cgtgatcgcg atgctggcac caacaacagt gtaggcaccc gtttccgccg
     2641 cctcataact actgatcaac tgctcgcgcc ccgcttcctc actcgtttta aagatctgaa
     2701 actcccgctc cacaacaccc atcttgccaa tcaaactctg tgtgctttcc ggactgggtt
     2761 cctgccctgc ccggatgtcg gacaacacct cattgagttg tatcatgtat tccttaaaag
     2821 caatgccgag ggcctcggtc ttgggactcc gtctgaaata actggttgcc agatccacgt
     2881 tgttcatcat cacacccgcc ggaccgatga ggtttgacca gcaaaatata tcgaccggag
     2941 gatgaatatc ataggcattc aagagcgcgg gaaattcgtg ctgatactgt tccagcgtac
     3001 gcacggagga ggtcaaagcg gccaaggttt gcaggttttc aactcgggct acctgctctg
     3061 tattcaggtc aaacacccca gcgtaatcct cacccaaggt tttgacgcat tgatttttta
     3121 tggcatcaaa ctgttgattg taagtttcca cactttctgg atcatcgggg tttatctgtc
     3181 cttgcagttc aagcaggccc atgagccgct ctcccatggg tccaaaggat tggccaatgt
     3241 aatagtcatt ccccacgttc agcgattgaa tcacaccgct gatgtagccc aactcacgac
     3301 ccccggcgaa ttcgcgcgaa atgatctcct caaaactgta tccctgcgcc ctaaagtcca
     3361 accccacctg ttgtttgaac tgggcccact cttctgggta ttcttgatag gaacgcagaa
     3421 gcgcctcaat ggcctcttca tccgtccccg tatcgagcaa acggtcaccc acgaaatttc
     3481 caaaaccggt atcaccttcg tgttctgcac tcacggcacc caggtgacag gcctcaaaaa
     3541 aactgtcaac gtcaccatac acaacggctg atggctgggt aatatcagta gtcacagccg
     3601 atgtgatttg accttctgac gatagcgtaa tggtctgtcc cttaaacaga ccagccataa
     3661 aatccctctg cgtggtggat agtttaacgc ctccaaagac gccatccatg aggtctttgt
     3721 gataatcctg tgcgcacaca gtgagtcccg catttgagtc cacagtggcg tagcccgaca
     3781 tcccgttgta taaccagttg caaaattgtg tgtcactggt cgcaaaacga atctggtcaa
     3841 aggcaacatc ttttgcctgt gccctatagg ttggctgacg ggacgtctct gtggccccca
     3901 atctgaggaa agaactggcg gacgcagagg ttaatagtcc cgacccgccc attcttttgc
     3961 tggagggaat gataaactct agctgatgtt tttgaccgta ctcatccaag gcgcggtaga
     4021 aggcgtcctg actgggcaca ccgctaaagt cggaggacga cgatacctca ccagacgcgt
     4081 aatcaaaaaa agcctgcatg accgaatcat cctgcgaggc gagccaggtc tcccaagcgt
     4141 caacatcttg ttcatcaaag acagtctgat cgggcatgtt aagggttacg gacatatatg
     4201 gtaattttac atgcaagatg taggccagac ctccagctgc gattgatata attaattgca
     4261 tcctatcaag atgttacgga aacatgccat agaaccgcat gataaatccg acaaattttt
     4321 gacaaaattg agaaaacgac aggcgttcga taaccagttg ccagcaatgt attattttct
     4381 tattactaaa aaatgaccga tcagctttcc ctcatcaccg ccctcccttt cattctcacg
     4441 ttgttatcga ttgctatcat ccctctggcc gcgccacact tttgggaaca caaccgcaac
     4501 aaggccgtca ttgccggcct gctggccaca cccattctgg tttatttttt ggggcatgac
     4561 gctcatctga tcctgaaaac cgtggaggaa tatttttcat tcattgttct tttgggctct
     4621 ctctatgtga ttgccggtgg catcaacatc agcggagatc tcaaggcgac ccccaaggtc
     4681 aatgcgtgtt ttttgggcat tggcgcgatt ctggcaaact tgattgggac tacgggtgcc
     4741 ag
//
LOCUS       DATDUN010000249         1692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_96750, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000249 DATDUN010000000
VERSION     DATDUN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96750"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VJC18_03295"
     CDS             <1..422
                     /locus_tag="VJC18_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44437.1"
                     /translation="TDVWGARWWKQTEGTYNASRDVTTVSKTLTHADGWSPPSKSQIL
                     PILSGWGFNNVFFPAINQVGLGNEPQEATTCSFNKIRSDVKVNDYDATSHQVTIDVRG
                     SVQTANSRYDTPNATMGYLLYCRYDAVCHVETGNTGT"
     gene            complement(481..>1692)
                     /locus_tag="VJC18_03300"
     CDS             complement(481..>1692)
                     /locus_tag="VJC18_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HLD44438.1"
                     /translation="NEVARHMGYSSFFALGVDEYDFTSDEMIAMMDKLLEEIKPLYRE
                     LHTYARYELAKRYNLPVPDYLPAHWLGNRWGQNWPGLVEGIDYNQGFKDKTSEWMIKT
                     AEKYYMSQGFPALSENFWKNSDLYELPADAKRKKNTHASAWHLNLDDDYRSLMSIKPD
                     YDWFTTIHHELGHIYYYITYAVPSVPPLLRDSPTSGFHEAITTFNQLAVSQRSYLTSY
                     GITEAKADSPDDDIRALLAQAFEFVVFLPFGAGTMPHFEYALYENNLDPSQFNKAWWD
                     YVKKYQGVVPPTERDEHYCDAASKTHINDYPVMYFNYAFAQVLAYQLNDHIAKNILKK
                     DPHNVNYFGQKEAGDFLRKMMVQGNTKGWRELLKETVGSELSAKPMLEYYAPLMTYLK
                     EQNKGRVYSEF"
BASE COUNT          407 a          445 c          431 g          409 t
ORIGIN      
        1 ggaccgatgt ctggggcgcg cgctggtgga agcagaccga aggcacctac aatgccagtc
       61 gtgatgtcac cactgtatcc aaaacgctca cgcacgctga cgggtggtcg ccaccgagta
      121 agagtcagat tcttcccatc ctttctggtt ggggattcaa caatgtgttt tttcccgcga
      181 tcaatcaggt gggactgggc aatgagcctc aggaagccac aacctgttcg ttcaacaaaa
      241 tacgttcaga tgtaaaggtt aatgattatg atgccacgag ccaccaagtc accatcgatg
      301 tgcgcggatc ggtacaaaca gccaactccc ggtatgacac acccaacgcc acaatgggat
      361 acctcctcta ttgcaggtac gacgcggtct gtcatgtgga gacgggaaac accgggacgt
      421 aaccctcaaa cggtccagcg accgcactca cgatttcaga gacgtataac gactaacagt
      481 tcaaaactcc gaatacacgc gccctttgtt ctgctcctta agatacgtca tgagcggggc
      541 gtagtattcc agcatgggtt tggcactcag ctcagagcca actgtttcct tgagcaactc
      601 ccgccagccc ttggtgttgc cctgcaccat catcttgcgt aaaaaatccc cggcctcttt
      661 ttggccaaaa taattcacgt tgtgagggtc ttttttaagg atgttcttgg caatgtgatc
      721 gttgagctga tacgccaaaa cctgcgcaaa ggcatagttg aaatacatga ccgggtaatc
      781 attgatgtgc gtcttgctgg ccgcgtcgca atagtgttca tcgcgctccg tgggaggaac
      841 gacgccctga tattttttaa cgtagtccca ccaggccttg ttgaactgac tggggtccag
      901 attattttcg tacaacgcgt attcaaagtg cggcatcgtc cctgcgccaa agggcagaaa
      961 gaccacaaac tcaaatgcct gcgccagcag ggcacgtata tcatcgtcgg gtgaatcggc
     1021 cttggcctcc gtgatgccgt agcttgtcag atacgacctt tggctcacgg ccaactggtt
     1081 gaaggtggtg atcgcctcgt ggaacccgga tgtggggctg tcgcgcaaca ggggaggcac
     1141 agagggcaca gcgtaggtga tgtaataata gatgtgcccc agttcatgat gaatcgtggt
     1201 aaaccagtcg taatcgggtt tgatactcat gagtgaacga taatcgtcat caagattgag
     1261 gtgccaagca gaggcatgcg tgttcttctt acgcttggca tcggcaggga gttcgtagag
     1321 atccgagttt ttccaaaaat tctcgctcaa ggcagggaac ccttggctca tgtaatattt
     1381 ttcggccgtc ttgatcatcc actcagacgt tttatctttg aacccctgat tgtaatcgat
     1441 cccctccacc aatcccggcc aattctgccc ccaacgattg cccagccaat gcgccggcag
     1501 ataatcgggc accggcaaat tgtatcgctt ggccagttcg taacgggcgt aagtatgcag
     1561 ttctctgtag aggggcttga tctcttccaa aagcttgtcc atcatggcaa tcatttcgtc
     1621 ggatgtaaaa tcgtattcat caacacccag cgcaaagaac gagctgtagc ccatgtggcg
     1681 tgccacttcg tt
//
LOCUS       DATDUN010000250         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_137638, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000250 DATDUN010000000
VERSION     DATDUN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137638"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VJC18_03305"
     CDS             <1..567
                     /locus_tag="VJC18_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HLD44439.1"
                     /translation="LQDLLTAFKMDVTKNRYDTFSDVLFYCQHSANPVGRLVLHLFGQ
                     SSLELFRLSDYICTALQLTNFWQDIAIDLKKNRVYLPQEELDKFDLPERALFNHTINV
                     NFKRLMQLQYDRTKDLFINGKPLGLMLSGKLGVEIRLTWLTGATILKRTHTANFDVFH
                     NRPQLTRFDFLRLFFVALSRKRYASFKI"
     gene            548..>2266
                     /locus_tag="VJC18_03310"
     CDS             548..>2266
                     /locus_tag="VJC18_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLD44440.1"
                     /translation="MPHSKSNHSSPSSEDTAPADIPHLLATCCADVTSVSSSNFIRAF
                     RFLPPAKTAALVKVYAFCRIMDDCVDEPTSQTHKQQALDYWHEQTRQMFARQPAHPVM
                     AELRKVIDEYKIPQEYFLGIIHGCAQDIVKTRYQTYPELLDYCYHVASLVGLICMKIF
                     GYVTPNAEKVAIDLGMAFQLTNIMRDLGEDYLKGRVYLAQEDLRQALYTEEDLKNKTE
                     NQALKQLLMLYHNRALAYYQTAFEEFEKDEHNKLIAARIMAKTYFKILQKMRRRGFRV
                     LYKRVSLNFFEKAWIMLSFSCKRGERAQGIRLKAHGLQPTANCQRPESDCQPLAVSCQ
                     PISSPVPRPSSLVRRSSALIVGGGVAGLYAAVLLDSLGYKVTLVEQKPILGGRAFSFT
                     DKITGATVDNGQHLLMGAYHETLAFLEAIGAKHTMQFAIPSRIPLWSRDHKINQFTLG
                     TWPPPFNLAKAVLGFWGMSLREKCGLVKMSGTIRKYPTQPSPNNQTAREWLKQFSQQE
                     TALANFWEIFTLATLNSTIDQAPAENLLQVLSHCFFAGRRDGFLVFPQQGLSDCFAHP
                     AQRYLTL"
BASE COUNT          555 a          672 c          525 g          514 t
ORIGIN      
        1 ctgcaggatc tcctgaccgc cttcaagatg gatgtcacaa aaaatcgtta cgacaccttc
       61 tccgacgtgc tcttttattg tcaacactcg gccaatcccg tggggcgcct ggtcctccac
      121 ttatttggac aaagctctct ggaattattt cgtctgtccg attacatctg cacggccctg
      181 caactcacca atttctggca ggacatcgcc atcgacctca agaaaaaccg cgtttacctg
      241 ccccaggaag agctcgataa atttgacctc cccgagaggg ccctgttcaa tcacacgatc
      301 aatgtcaact tcaaaagact catgcaattg cagtacgaca ggacaaaaga cctttttatc
      361 aatggcaagc ccttggggct catgttgtcg ggaaaactgg gagttgagat ccggctcacc
      421 tggctcacgg gggctaccat cttgaaacga acccacaccg ccaatttcga tgtcttccac
      481 aaccgtcccc aattgacccg atttgatttc ctcagattgt tcttcgttgc cctcagccgt
      541 aaacgctatg cctcattcaa aatctaatca tagttccccc agcagcgaag acaccgcacc
      601 cgcagacatc ccccatctcc tggccacctg ttgtgccgat gtgaccagcg tcagttcatc
      661 caacttcatc cgggccttcc gctttcttcc ccctgcaaaa acagcagcct tggtcaaggt
      721 gtatgccttc tgccgcatca tggacgactg tgtggatgaa ccaacttcgc agactcacaa
      781 acaacaggca ctggattatt ggcacgaaca gaccagacaa atgttcgccc ggcagcctgc
      841 tcatcccgtc atggcagaac ttcgcaaagt gatcgacgaa tacaaaattc cccaggaata
      901 ttttttggga attatccacg gctgtgcgca ggacatcgtc aaaacacgtt accagactta
      961 tccggagctg ttggactatt gctatcacgt ggccagtctc gtgggactga tctgcatgaa
     1021 gatattcggc tacgtgacac ccaacgccga aaaagtggcc attgatctcg ggatggcgtt
     1081 tcaactcacc aacatcatgc gcgacctcgg tgaggattat ctcaaaggac gcgtgtacct
     1141 cgcgcaggaa gacctgcgac aggccctcta caccgaagaa gacctcaaga acaaaacaga
     1201 aaaccaggcc ctaaaacaat tgctaatgct atatcacaac cgcgcactcg cctattacca
     1261 gaccgccttt gaggaatttg agaaggacga gcacaacaag ttgatcgcag ccaggatcat
     1321 ggccaaaacg tatttcaaga tcctgcaaaa gatgaggaga cgcggtttcc gggtattgta
     1381 caaacgagtc tcactcaatt tttttgaaaa ggcgtggatc atgctctctt tctcgtgcaa
     1441 acgtggggaa agggcgcaag gcataaggct taaggcgcac ggcttacagc cgactgccaa
     1501 ctgccaacgg ccagaatctg actgtcagcc gttagccgtt agctgtcagc caatttcgtc
     1561 ccccgtccct cgtccctcgt ccctcgtccg tcgttcctcc gctctcatcg tcggtggcgg
     1621 ggtcgcgggt ctctacgccg cggtgcttct ggattcactg ggatacaaag tcacccttgt
     1681 ggaacaaaaa cccattttgg gaggcagggc cttttccttt accgacaaga tcactggcgc
     1741 cactgtcgat aatgggcaac acctgctgat gggggcgtat cacgaaacgc tggcttttct
     1801 ggaggccatc ggagccaagc acacaatgca attcgcgatc ccttcccgta tcccactatg
     1861 gtcacgggat cacaagatca atcaattcac gctgggcacc tggccacctc cgttcaatct
     1921 ggcaaaggcc gtgcttggtt tctgggggat gtccctgcgt gagaaatgcg gattggttaa
     1981 aatgtctggc accatcagaa agtacccaac acaaccttcg ccaaacaacc agacggcccg
     2041 ggagtggctg aagcagtttt cgcaacagga aaccgcgctt gccaatttct gggaaatctt
     2101 cacattggcc acgctcaact ctaccatcga ccaggccccg gccgaaaatc tgctgcaggt
     2161 attgtcccac tgtttttttg cgggaaggcg cgacgggttt ctggtcttcc cccagcaagg
     2221 cttgagcgat tgctttgccc atccggcaca gcgctacctc accctg
//
LOCUS       DATDUN010000251         6419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_427794, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000251 DATDUN010000000
VERSION     DATDUN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427794"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VJC18_03315"
     CDS             <1..403
                     /locus_tag="VJC18_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44441.1"
                     /translation="NTYNGIFVESINHISAHIAWDETEVAFVIDDNDFWVNSGASLTL
                     GDDVALKFRPDSVLLLEDGTSQLLVAGGVNDKESTVVFTSYKDDTVKGDTNADGSTTS
                     PAVGDWGGIYDDTADAPYYLSWSNIYYDELH"
     gene            1028..1759
                     /locus_tag="VJC18_03320"
     CDS             1028..1759
                     /locus_tag="VJC18_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLD44442.1"
                     /translation="MFWLNNISHTFPLQTTPVLHNITLNIPQGSLTAIMGLNGSGKTT
                     LLKVLSSLILPRQGEVFIAGQNATAYKAKELAQHIAFVPQDFPTDFPFTVNEFVLMSR
                     YAWQTKLFDGQEDQIKIDRVLRELNLWDLKDRLITTLSGGERQRVLLARAFAQETKAI
                     LLDEPCNHLDIKNKHELLTWLKREHTRSSKTIIAVMHDFQDVNRHFSDVLLLKHGQLA
                     FWGAKKKALEPERLRAIFETDAVCC"
     gene            complement(1921..2172)
                     /gene="spoVG"
                     /locus_tag="VJC18_03325"
                     /pseudo
     CDS             complement(1921..2172)
                     /gene="spoVG"
                     /locus_tag="VJC18_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
     gene            complement(2322..3191)
                     /gene="ispE"
                     /locus_tag="VJC18_03330"
     CDS             complement(2322..3191)
                     /gene="ispE"
                     /locus_tag="VJC18_03330"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HLD44443.1"
                     /translation="MKVVKLRSPAKINVRLDVLNKRQDGYHDLRMINTPINIYDEIEC
                     ELTEKGIQVECENDATVPDGDQNIVYQVAKEILAYSNKNIGVRIKIYKNIPSAAGMGG
                     GSSNAAAVLTGLNELLKINLSKEKLMKIGLRFGADVPFFVFGSSAIARGVGENLVKIK
                     KMPKLPLVIISPNISVSTKWVYEHYEAANDNRPQIDESKLEEIPHQFATKKSVIKFLN
                     NDLESVTLKKYPLVEDLKKILRKTGALAAQMTGSGPTVFGIYPNKEIAEVACNKIKAN
                     ITDCRVFLAENIT"
     gene            complement(3411..4553)
                     /locus_tag="VJC18_03335"
     CDS             complement(3411..4553)
                     /locus_tag="VJC18_03335"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HLD44444.1"
                     /translation="MLTFFDEDESVLIRGLIKGGALENPQKKDSVWENESRSCWLLSL
                     LALGLFFSGPFVFLTPLPFIYYQLRHKRSPFLSIVWPSVLAVVLVYFLGLDFFHELYQ
                     AHPNAYWLLPIPFLDMVAFFSKPTVIFLGVSSFVVCLVVSFFITKSLLGPKQHVFKRL
                     FWAIFGVFAACVFLGVVVLLPQGDLFIVAFEKALQQGLNEFIAAKESAGLMSVSQIID
                     FKSQIADYVGRAAYVIPFLVMVSLIILFVVNLIIAKRFFANSFPELKKINLTFFCLPF
                     EVVWVVLALIALMVINEFTADATLLFYIGVNSLLVFAVIYFFSGLAIFIAVLDQKKIF
                     GFLRIMFVVMAILMREFSLCLFVILGFADHWLDLRKLESKQTPSLS"
     gene            complement(4691..4963)
                     /gene="rpsR"
                     /locus_tag="VJC18_03340"
     CDS             complement(4691..4963)
                     /gene="rpsR"
                     /locus_tag="VJC18_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HLD44445.1"
                     /translation="MFIKKKKEGKYPLKKTSSRRKACRFCSDPENPLDFRRPKMLAGF
                     LTEEGHIIPRRMTGNCQFHQTRLVEAIKRARHVALLPYTVNHAIRD"
     gene            complement(5044..5394)
                     /gene="rpsF"
                     /locus_tag="VJC18_03345"
     CDS             complement(5044..5394)
                     /gene="rpsF"
                     /locus_tag="VJC18_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HLD44446.1"
                     /translation="MTQTRQYETVCVLLPDVQGDQLKTVEEKIQKIFSNQKIENVTKK
                     DWGIRRLAYPIKRNKSGRYFEFSYTAPTEAITGLEKNLGYEESVLRFLTIKQTKQSPK
                     NVQVEPDGFDFIEY"
     gene            complement(5530..5724)
                     /gene="rpsU"
                     /locus_tag="VJC18_03350"
     CDS             complement(5530..5724)
                     /gene="rpsU"
                     /locus_tag="VJC18_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HLD44447.1"
                     /translation="MPGVSIRDGESFEAAVRRFKKQCEKAGILAEVRKRECFEKPSIQ
                     KKKKQAQARKRVSLMSRRER"
     gene            complement(5884..>6419)
                     /locus_tag="VJC18_03355"
     CDS             complement(5884..>6419)
                     /locus_tag="VJC18_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLD44448.1"
                     /translation="KLTVSHLLLTHGHIDHIAACGELAREMNAKICLHQDDEQLFDGL
                     KDQARQFGVAVRGEHNTVDHYLKDGEALITQEGLTLKVLHTPGHSPGSVCFYADDFTL
                     TSPKGIIEHIPLLCCGDTLFLGSIGRTDLWGGDHRALISGVKNKIFTLPDDTLVIPGH
                     GPTTSVGQEKATNSFFQ"
BASE COUNT         1788 a         1632 c         1316 g         1683 t
ORIGIN      
        1 caacacctac aacggaattt tcgttgagag cattaaccac atcagcgcac acatcgcctg
       61 ggatgagaca gaagtggcct ttgtgatcga cgacaatgac ttctgggtca attctggcgc
      121 ctcactcaca ctcggagatg atgtggccct caaattcaga cctgacagcg tgttgctgtt
      181 agaagatggc accagccaac tgcttgtggc tggcggtgtg aacgacaagg agagcactgt
      241 tgttttcacc tcctacaaag acgataccgt caaaggcgac acgaatgccg atggctcaac
      301 cacctcgccc gctgttggtg attggggcgg catttacgac gacaccgccg acgccccgta
      361 ttacttaagc tggtccaata tttattatga cgagcttcat tgaagaatga aacaatcaat
      421 gtcccgcaga gcgggatcag tcctacggct gaaaaaaatg aatcaatgaa gaaaaaaaac
      481 gatggggaaa ggtcggatac aaaacctctt tgattggtaa atttgtggcc tgccctcccc
      541 acaccacctg gggactaaaa aagacaaaca gacttatcct cataatacca acaagttatt
      601 gacacgtttt ttttaagtca gttggcaccg cgtatgcata ttaagactgt gaaagtcgac
      661 ccaattcctc ttaacggagg aaaccctcat tacccctcaa ggccggttag agcccccctc
      721 tttagccggt ctttcctttt ttacaaccat ctcttgtaaa agggacctct gttggttttt
      781 ataaatgtcg aggaccgcat catcgaatag tgccattttg cgaacaccat agccacaatg
      841 acccctcgtg gattgccacg cctcctcact ctcgttcggg gctggccacg acgccacatc
      901 tgcaaaatgg cacaagaaca caaaaaacaa aagatgcctt cgcagacatt gataaaaacc
      961 aacagaggaa aacacttttt tccatttccc tattgcgacc aatgatacga ttgaatagtg
     1021 tcacaccgtg ttttggctca acaacatctc acacaccttc cccctccaaa ccactcccgt
     1081 cctacacaac atcacgctca atatccccca gggctcgctg acagccatca tgggccttaa
     1141 cgggtctggc aaaacaaccc tgctcaaggt cctcagctcc ctcatcctcc ccagacaggg
     1201 cgaggttttt atcgcgggac aaaacgccac ggcctacaag gcaaaagaac ttgcccaaca
     1261 catcgccttc gtgccacagg attttccgac agactttcct tttaccgtta acgagtttgt
     1321 cttgatgtct cgctacgcct ggcaaacaaa actgtttgat ggtcaagaag accaaatcaa
     1381 aatcgatcgc gtcctgcgtg agctcaatct ctgggacttg aaagaccgcc tcatcaccac
     1441 tttatcagga ggggaaagac agcgcgttct gttggcacgc gccttcgccc aagaaacaaa
     1501 ggccatccta ctggacgaac cctgcaacca tctggacatc aagaacaaac acgaattgtt
     1561 gacctggctt aaacgcgaac acacgcgctc atcaaaaacc atcattgccg tcatgcacga
     1621 ttttcaagac gtgaatagac acttcagcga tgtgctgtta ttaaaacatg gccaacttgc
     1681 tttttgggga gctaaaaaaa aggccctcga acctgagcgg ctgagggcca tctttgaaac
     1741 tgatgcggtg tgttgctaac aaccggcact tttttatttt ttcaggccga gccctgtcgc
     1801 tggtacctcg ccactgttca tggcggcaac actgggcata tcgtcgttcg ccgcaatcct
     1861 tctttcgtaa tcgatgtcgt cgtcgcactc tctcctgacg ggaaaacccg ccttgaccat
     1921 cttgatctcg tcattaaact tatcgaggat atgtttttcg agataatttc tgaaatctga
     1981 gttgatggga tgcgcgatgt ctttgtacga tccgtccttc ttctttttgg acggcatggc
     2041 cacaaagagt cctgtgttcc cgttgatcac cttgagatcg cgaacaacaa aacaatcgtt
     2101 gaaggtcacg gtgacatacg ctttgagtcg ttcttcatcg gcaagaaaaa ttctggtttc
     2161 ggtcacttcc atcatgcaaa ttcctccaat cacgcaacat gcgaatcgca agtatcactt
     2221 accatgtcgc caaaatatga caagtgattt cgtcaagaca gaagaaacgc tctctgaatg
     2281 ttgttatgat gaatctgcaa atttccagta gcctaatgac gtcaggtgat attctcggcc
     2341 aaaaacacgc ggcaatcagt aatgttggcc ttgattttgt tgcaggccac ctctgcgatt
     2401 tctttgttgg ggtaaatccc aaacacggtc ggccctgacc ccgtcatctg cgccgccaag
     2461 gcgcccgtct ttctcaagat ttttttgagg tcttccacaa gcggatactt tttgagcgtc
     2521 accgattcca gatcattgtt caggaatttg atcactgatt ttttcgtggc aaattgatgg
     2581 gggatttctt ctaatttgct ttcgtcaatc tgcggtcggt tgtcattagc cgcctcatag
     2641 tgctcataca cccatttcgt ggacacggag atgttgggtg aaataatcac caagggcagt
     2701 ttgggcattt tttttatctt caccaaattt tcacccacgc ctctggcaat ggcggaggat
     2761 ccaaaaacaa agaaaggaac atctgcccca aagcgaagac caattttcat caacttttct
     2821 ttggaaagat taatctttaa tagttcattg agcccagtca acacggccgc cgcattgctg
     2881 gacccacctc ccatcccggc cgcggacggg atgttcttgt aaatcttgat gcgcacaccg
     2941 atatttttgt ttgaataggc caaaatctct ttggccactt ggtacacaat gttctggtca
     3001 ccatcaggaa ccgttgcatc attttcacac tcaacctgaa tccctttttc tgtcaactcg
     3061 cattcgatct catcgtagat gttgatcggc gtattgatca tcctcaaatc gtgataacca
     3121 tcttgtcttt tgtttagtac atccagcctg acattgatct tcgcaggcga cctgagttta
     3181 acaactttca tgcctgtgcc cctttcggac tactttgcgt aaggaatttt tgaaaaatag
     3241 tttttataaa aattccttct tcaaaaattc acgtacaata tatcttcgag ggccgatgat
     3301 cgcttttttc gacaaccgac ccctcagcgg catacaaaaa aacattgatt tttgtcaatg
     3361 tagggatgct ttcatccaaa caccatgata tccttacaaa aaccattttt tcagcttaaa
     3421 gatggggtct gcttgctttc tagtttcctt agatctaacc aatgatccgc aaaccccaaa
     3481 atcacaaaca gacacaacga aaattccctc attaaaatgg ccatgacgac aaacattata
     3541 cgaagaaaac caaatatctt tttttgatcc agaacggcga tgaagatagc cagtccactg
     3601 aaaaagtaga tcacggcaaa aacaagcaag gagttcacgc caatgtaaaa caacagggta
     3661 gcatctgccg tgaactcatt gataaccatg agggctatca gggccagcac aacccacacc
     3721 acttcaaagg gaagacaaaa aaaggtaagg tttatttttt taagttctgg aaatgaattt
     3781 gcaaaaaatc ttttggcaat gatcagattg acaacaaaca aaatgatgag cgacaccatc
     3841 accagaaacg gtatgacgta agcggcccgg ccaacataat cagcaatctg actcttgaaa
     3901 tcaatgattt ggctcacgct catcagtcca gcactttctt tcgccgcaat gaattcgttc
     3961 aatccttgtt gcaaggcttt ttcaaaggcc acaatgaaca aatctccctg cggcaacaaa
     4021 acaaccactc ccagaaaaac acatgccgca aaaaccccaa agatagccca aaacaatctt
     4081 ttaaaaacat gctgtttggg accaagcagg gatttggtga taaaaaaact aaccaccaaa
     4141 cagacgacaa atgaactcac acccaaaaaa atcacggtgg gcttactgaa aaaggcaacc
     4201 atatccaaaa aaggaatggg caataaccag taggcgttgg ggtgcgcctg gtacagctca
     4261 tgaaaaaaat ccaggcccaa aaaatacaca agcacaaccg ccaagacact cggccagacg
     4321 atggataaaa aaggactcct tttgtgtctg agctgataat agatgaatgg cagaggggtt
     4381 aaaaagacaa aggggcccga gaaaaacagg ccgagtgcca acaggctcaa cagccagcag
     4441 gaacgcgact cattttccca aacggaatct tttttttggg gattttccaa agcgcccccc
     4501 ttgataagac cgcggattaa gacactctca tcctcatcga agaacgttaa caatcgaatc
     4561 cttcgaacca ctagtacatc gactcatcaa tttgtttaca aatttattcg ctcagtacca
     4621 gtagtgagtg agcaaaaata ttactgctat tatttgttaa attcgtaaat attttttatg
     4681 aatcgaacta ctagtcacga atggcgtggt tgaccgtgta gggcaacaat gccacgtgcc
     4741 ttgccctctt gatggcctcc accaaacgcg tttggtgaaa ctggcagttc ccagtcatgc
     4801 gacgcggaat gatatgtcct tcttccgtta aaaaacctgc cagcattttg gggcgacgaa
     4861 aatccagggg gttttcggga tcactacaga aacgacaagc cttacgcctt gaactggttt
     4921 tcttgagggg gtatttccct tcttttttct ttttgataaa catacaacaa acctttcctt
     4981 tttgctttca cactcattaa attttctgtc gcactgccga ccagcactaa ccactttaat
     5041 cattcaatat tcaatgaagt cgaatccatc cggttccacc tgaacatttt tgggtgactg
     5101 ttttgtttgc ttgatggtca aaaacctcag cacagactct tcgtacccca ggtttttctc
     5161 cagacccgtt atggcctcag tgggtgctgt ataagaaaat tcgaaatagc gcccggattt
     5221 gtttcgtttg atcgggtacg ccaaacgtcg gatgccccaa tcttttttgg tgacattttc
     5281 gatcttctgg ttactgaaga ttttttgaat tttttcttct actgttttta attgatctcc
     5341 ctgtacgtcg ggaagcagaa cgcacacggt ttcgtactga cgcgtttgtg tcattgtttc
     5401 tccttttgga tatcaagcct gttctgcgtc aaacacatca gcccgtagcc aactttgtat
     5461 cagcctgctg aagcttgctc agaacaagca aggctatgac aaacaccaac caaattgtcg
     5521 tgtacctctc tacctctctc ttcgagacat gagagataca cgcttacgtg cttgggcttg
     5581 ttttttcttt ttttgaatgg aaggtttttc gaagcattcg cgtttgcgaa cctcggccaa
     5641 aatgccggcc ttctcacact gttttttaaa acgacgaacg gcggcttcaa aagactcgcc
     5701 atctcttata ctgactccag gcataaatgc tcacctctta aatgatgata aaccgcctct
     5761 caggaaacgg cccgttttca aacggtcggg accaatacga taaatagctt cgacaagtca
     5821 aatctttcgg gccaacattt accaggacac ctcaataatc ggataatcct aaacaatatt
     5881 atgtcattgg aaaaaagagt tggtcgcttt ttcctgccct acactcgtgg taggcccatg
     5941 cccgggaatc accaacgtat cgtctggcag agtgaaaatt ttgtttttca cgccactgat
     6001 caacgctcgg tggtcgcccc cccacaaatc tgtccttccg attgagccca aaaacagggt
     6061 gtccccacaa caaagcaggg gaatgtgttc tatgatccct ttgggggaag tgagcgtaaa
     6121 atcatccgcg taaaaacaga cagagcccgg tgaatgaccc ggtgtgtgga gaaccttgag
     6181 ggtcaggcct tcttgtgtga tgagtgcctc accgtctttc aaataatgat caacggtgtt
     6241 gtgttcgcca cgcacagcga caccaaattg acgcgcctga tccttaaggc catcaaagag
     6301 ttgctcatca tcctgatgca gacatatttt ggcgttcatc tcccgtgcca gttcgccgca
     6361 ggccgcgatg tgatcgatgt gtccatgcgt gaggagcaaa tgggagacag tgagttttt
//
LOCUS       DATDUN010000252         1006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_427840, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000252 DATDUN010000000
VERSION     DATDUN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427840"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="nadA"
                     /locus_tag="VJC18_03360"
     CDS             complement(<1..781)
                     /gene="nadA"
                     /locus_tag="VJC18_03360"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HLD44449.1"
                     /translation="MLQKILELKKCKKALIIAHYYQTPDIQDIADYVGDSLGMAIFAK
                     ETDYDLAVICGVKFMAETFKVMNPKKKILVPDLNAGCSLADSCTPAIFEYFKSKHPGA
                     FVMTYINSSLEIKAMSDVIVTSANAVKIARQVPPHQKIIFGPDQHLGRYVAKLCGREL
                     ILFPGNCFVHTSFSARALHEICQQNPDAVLIAHPECEEVLLSQAQFVGSTGQMLKFTK
                     DNPAKKFIVLTEAGILHQMQKASPEKEFIMGPDLSGCACNVC"
BASE COUNT          270 a          245 c          237 g          254 t
ORIGIN      
        1 gacacacatt gcaagcacac ccgctcagat cgggccccat aatgaactcc ttttcgggag
       61 aagccttttg catctgatgc aaaatccccg cctcggtcag cacaatgaat tttttggctg
      121 gattatcttt ggtaaatttt aacatctgcc ctgtggaccc cacaaactgt gcttgcgaga
      181 gcaacacttc ctcacattca gggtgcgcga tcaggaccgc atctgggttc tgctggcaga
      241 tttcgtgcaa ggctcgcgcc gaaaatgagg tgtgcacaaa acaattgccc ggaaacaaaa
      301 tcaactcgcg cccgcatagt ttggccacat agcggcccaa atgttgatcc ggaccaaaaa
      361 tgattttttg gtgtggtggc acctgtctgg caatcttcac agcgttggcc gaagtcacaa
      421 tcacatcgct catggccttg atttcgagac tggaattaat gtacgtcatg acaaaggcgc
      481 cgggatgttt tgacttgaaa tattcaaaaa tggcaggggt gcacgaatca gccagcgaac
      541 aacccgcatt caaatcagga accagaattt tctttttggg gttcatgacc ttgaacgtct
      601 cggccatgaa cttgacccca caaatcacgg ccagatcata atcagtttcc ttggcaaaaa
      661 tggccatacc cagactgtct cccacataat ccgcgatgtc ctgaatgtcg ggggtttgat
      721 aataatgggc tatgatgagg gcttttttgc attttttgag ttcaaggatt ttttgtaaca
      781 taggcgactg acattgtaac tcaaataaca aaaaggaatc aagaaccact ccatgatgac
      841 ctgaccgcgc ctctccttat caggagacag aagttcttaa ataataagcc gttagagcaa
      901 cgagtgggac ccactggttt ttgtgattgt ggaggcccga ctgcttgcag ttgggaggct
      961 ccacgatctc aaaaaccaac gagggtacga agttgctctg gggctt
//
LOCUS       DATDUN010000253         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_137705, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000253 DATDUN010000000
VERSION     DATDUN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137705"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VJC18_03365"
     CDS             complement(<1..373)
                     /locus_tag="VJC18_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44450.1"
                     /translation="MNRFGLASPTRHLPGIPCTSAVFQQPFFKTESARYTRRAVALPD
                     GRRFLNPFQIHGKDLVFMTGLARRCAVQKVLDEQGVSLTPVSLGFGQTAMGVAITDFS
                     ANSLAQDPDNPTAPFDPHSTGY"
     gene            complement(426..1541)
                     /locus_tag="VJC18_03370"
     CDS             complement(426..1541)
                     /locus_tag="VJC18_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLD44451.1"
                     /translation="MVIWRSTPTALVLGGTGHIGANIALQLARQGYPTTTVSRRPVDC
                     IKSPIRNAFEQHGIQHHSIDVYGAENERPLLRLLEQYDLVINAAEPYPVNPHEIAASV
                     SGVARLLSAAHNAGFGQAPKKWIRIGSPPAEIPNPTLSESDNGDRLYPENMSWGILRK
                     RFHPAFSNPYFQTKLQIKQLFDHAVACGQIRGITVAPTAVVGWGADHGAWEPITNYFN
                     RRFPPIIPAIPVDTIPVDHLAKGVVLAIEKGAIGEIFQLSGVHTNMADVILEGLKYAG
                     MNQLPVSINLPRALWGPTLLGEVVMLRGFELFLTSQGLTPPAICRPMTPALSMMMGLR
                     HSRKAQDQLGYQLPTEEDLIRAIHASVDWFRETGEIK"
     gene            complement(1802..>3354)
                     /locus_tag="VJC18_03375"
     CDS             complement(1802..>3354)
                     /locus_tag="VJC18_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLD44452.1"
                     /translation="TLASSRHHYLRQETKDYVPKILAALHIVKNYSSFGYTSSDFDTP
                     MQYDRVTVPDATDIKAIAKSARTTPKVIAAMNPSLVLGITPPGPSSIYVPKGAASEFE
                     RNYAMIPVSKRVTSLQYKTGAHESLTGIAKKYGLSATKLAKLNNIQPARSNRELKKGT
                     LINMPGDQHTLNSIAKLSASAIASSGKPRIQTKTVYYKVRAGDTLSKIAKRNKTSARK
                     LAQWNGMRSSSPLKIGQKIKLQNATYTAPANGGFLAKFTPEPESQERLSGVSHIIVQE
                     TQGGNQFIPSEPEEIEIPNLAAAEDSFAPQEDPLLPAFVKTMDGEVLAYGKKLEEEEY
                     VVQKPAPTVYHVVLPGETLLSIAGKYKMTVEGIKQLNNLESNLIRSNQKIIVSQPSQK
                     QMASAQKTDRKATTKTAYTVKSGDTLSVIAKRNHVSVDKLKAWNNMTGNTIQPGQKIL
                     VQNAEQKTSSRPVAQNDKKVIYHKVQAGETLWSLSRKYDVKISDIMKWNALNTQTVKT
                     NQKIKIIA"
BASE COUNT          699 a          741 c          963 g          951 t
ORIGIN      
        1 ggtaccctgt tgaatgggga tcaaagggtg ctgtggggtt atcgggatct tgagcgagag
       61 agtttgcgga aaagtcggtg atggccactc ccattgctgt ttgaccaaaa cccaagctca
      121 caggggtcaa agacacgccc tgttcatcca atactttttg gacagcacat ctccgggcaa
      181 gcccggtcat gaagacaagg tcttttccat gaatttggaa agggttcaaa aatctgcggc
      241 catcaggcaa tgcgaccgct ctgcgtgtgt accttgcgga ctctgttttg aaaaaaggtt
      301 gttgaaaaac agcgctggta caggggatcc cggggagatg ccgtgtgggt gaagccagtc
      361 caaatctatt cattaatgtc gctatagggt gcgtcaacat aggctgtctc ctaaaagggc
      421 tgtgtctact tgatttcccc tgtttccctg aaccagtcga ccgatgcgtg aatggcgcgt
      481 atgagatcct cttctgtggg aagttggtag cccagttggt cttgggcttt tcgagaatga
      541 cgtaatccca tcatcatgct gagtgcgggg gtcatgggtc tgcagattgc cgggggtgtg
      601 agtccttgac tggtcagaaa gagctcaaaa cctctcaaca taacgacctc accgagcagg
      661 gttggccccc atagcgctct tggaaggttt atgctcacgg gaagctgatt cattccagcg
      721 tattttaatc cctccagaat gacatccgcc atatttgtgt gaacgccact taattgaaat
      781 atttctccga tcgcaccttt ctcaatggcc agaacaaccc ctttggcaag atgatccacg
      841 gggatggtgt cgactggaat ggcgggtatt atgggaggaa aacgccggtt gaaataattt
      901 gtaatgggtt cccatgcacc atgatccgct ccccaaccca caacggccgt gggggctacg
      961 gtgatgcccc tgatctgacc acaggccacc gcatgatcga agagctgttt gatctggagc
     1021 ttggtttgga aatagggatt ggagaaggct gggtgaaaac gttttcgcaa aattccccat
     1081 gacatgtttt cggggtaaag tctgtcgccg ttgtcgctct ctgatagcgt gggattgggt
     1141 atttctgcgg gcggggagcc aatgcggatc catttttttg gcgcttgacc aaatccggca
     1201 ttgtgagccg ccgaaagtag gcgagcaaca cctgagacgg atgctgcgat ttcatggggg
     1261 ttgacagggt aaggctcagc cgcgttaatc accaaatcat actgttcgag gagacgtaag
     1321 agagggcgtt cattttctgc cccataaaca tcaatcgaat gatgttgtat tccgtgttgt
     1381 tcaaaggcgt ttctgatcgg ggacttgatg caatcaacgg gacgcctgct tacggtggtt
     1441 gtgggataac cttgtctcgc caactgcagg gcaatgttgg cgccgatgtg gcccgtgcct
     1501 cccagaacaa gagccgttgg tgttgatcgc caaattacca tggctgtttt atcgtgccat
     1561 gagatgaaaa gttgcgttct atttttacta aattgcagct gtggaatcta gaaaaccgtt
     1621 caaatcgttg aagatcaagg cgctcaggga aaattaaacc gcaacatact tatgatggta
     1681 tgtgaggatt taatttttca tgagcaacgc ggagatccgg cgatttcaac ggttttccct
     1741 gtacacaaat gtgcgctcag gaaatccgac tgagcaggga ggaagcggat ggaaggtaac
     1801 gtcaagcgat gattttgatt ttttgattcg tcttgactgt ctgggtgttg agcgcattcc
     1861 acttcatgat gtcgctgatc ttcacgtcgt acttgcgcga cagggaccac agggtttcac
     1921 ctgcctgcac tttgtgatag attacttttt tgtcgttttg cgcgacggga cgcgaggacg
     1981 tcttttgttc cgcgttttgc accaggattt tttgccccgg ctggatcgtg ttgcccgtca
     2041 tgttgttcca ggcctttagt ttgtccacag agacgtgatt gcgttttgcg atgacggata
     2101 aagtgtcacc agactttacg gtgtaggctg tttttgtggt cgccttccgg tcggtctttt
     2161 gagcagacgc catttgtttt tgagaaggtt gtgagacgat gattttctgg tttgagcgaa
     2221 tgaggtttga ttccagatta ttgagttgct ttattccctc cacagtcatt ttgtatttgc
     2281 cggcgatgct cagcagtgtt tcgccgggca gcaccacatg atacactgtt ggcgctggct
     2341 tttgcaccac gtattcctct tcttccagct tcttgccata ggccagaacc tcaccatcca
     2401 tggtcttgac gaacgcaggc agaagggggt cttcctgggg ggcaaaactg tcttcggccg
     2461 ctgccagatt ggggatttcg atctcctcgg gttcagaggg aatgaattga ttcccgcctt
     2521 gtgtttcctg cacgatgatg tgacttacac cggaaaggcg ttcctgactt tcgggctcgg
     2581 gtgtgaattt cgccaggaaa cctccgtttg caggcgctgt gtaggtcgcg ttttgcagtt
     2641 tgattttttg tccgattttt aagggagagc ttgatctcat gccgttccac tgtgcgagtt
     2701 ttctggcaga ggttttgttg cgtttggcga tttttgagag ggtgtctcct gcgcgtacct
     2761 tgtaataaac ggtcttggtc tggattctgg gttttccgct cgacgcgatg gcactggccg
     2821 aaagcttggc gatggagttt agggtgtgtt ggtctcctgg catgttgatg agggtccctt
     2881 ttttgagttc acggttgctc cgtgcaggtt gaatattgtt gagcttggcc agttttgtag
     2941 cactcaaacc atattttttg gcgatgcctg tcaatgattc gtgggcccca gttttgtatt
     3001 ggagtgacgt gacccttttt gagacgggga tcatggcata atttctttcg aactcactgg
     3061 cggccccttt ggggacgtag atagatgagg gaccaggggg ggtgattccc agaaccaggg
     3121 aagggttcat ggccgcaatg accttgggag tggttctggc acttttggca atggccttga
     3181 tgtcggttgc gtcggggaca gtgaccctgt cgtactgcat gggtgtgtcg aagtctgacg
     3241 aggtatagcc gaaagaggaa taatttttga cgatgtgcag ggccgccaga attttgggga
     3301 cgtagtcctt ggtttcctgc cttaagtagt gatgacggga cgacgccagg gtcc
//
LOCUS       DATDUN010000254         2769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_262327, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000254 DATDUN010000000
VERSION     DATDUN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262327"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VJC18_03380"
     CDS             <1..492
                     /locus_tag="VJC18_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44453.1"
                     /translation="IGWDAFAETQRLLTGRPDKQIVGINKNNLKQFVLGSGKVTITYK
                     DGHSETMTKEKLNITIGALYLHRCLTHYNPTGSIALALANYNWSDTNMIYYHMPYYFG
                     ITTSPFSNRRGQLQTHTALDRQPLEYIAKYLVLQQMFKDTIFAQAIKKEDERKRNLVL
                     AAL"
     gene            625..1602
                     /locus_tag="VJC18_03385"
     CDS             625..1602
                     /locus_tag="VJC18_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HLD44454.1"
                     /translation="MKDMKITDFIKTHYRHFNAATLVDAAEAYKQHLNQGNKMLVTLA
                     GAMSTAELGISLAEMIRQDKIHAICCTGANLEEDIFNLVAHEHYVRVPHYRDLTPQDE
                     QKLLDRHLNRVTDTCIPEEEAFRRLEHTVLAEWTGAEKNNQRFFPHEFMFRMLRSGAL
                     KKYYQIDPKNSWMIAACEKNLPLFVPGWEDSTLGNIFAAHNIDGSIKNPHVVKTGIEY
                     MMELARWYTETAPQTSIGFFQIGGGIAGDFPICVVPMLHQDLQRSSVPLWGYFCQISD
                     STTSYGSYSGAVPNEKITWGKLGIDTPKFIIESDATIVAPLMFAYILEQ"
     gene            1621..1926
                     /locus_tag="VJC18_03390"
     CDS             1621..1926
                     /locus_tag="VJC18_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44455.1"
                     /translation="MLLQRDKKQDPIDAAKVRIEAESVLEMVLLVIKSETQNQTQRSL
                     PEESRLKGLTEDLKSAVTGGDTCKMKESIMAIDHFMASCGEQIKQDIESALPRKKIS"
     gene            1923..>2769
                     /locus_tag="VJC18_03395"
     CDS             1923..>2769
                     /locus_tag="VJC18_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HLD44456.1"
                     /translation="MNLKAFHYDLPEELIAKYPCDRRDESRLLLLDKTTGKTTHTIFK
                     DMATLFNKGDVLVFNNSRVFPCRLITKRKGGGKQEIFLVRKVEDKTRRSWIVNRGSSI
                     NNIAIIKRHTSDTTSNQFANQISKNTDVPLTVRSAIHDSRSTIHEFWTILINVSRKVR
                     EGDVFDFDDFTITICDSAGAERLALIEFEGKLMPLLEKHATVPLPPYLNRVADETDRT
                     RYQTIYASQNGSTAAPTAGLHFTPELMDTLKERGVIMANVTLHVGPGTFLPVRVDNIE
                     NHKMHS"
BASE COUNT          826 a          758 c          583 g          602 t
ORIGIN      
        1 attggctggg acgcgttcgc agaaacgcaa agactgctca cgggtcgacc agacaaacag
       61 attgtgggca tcaacaaaaa caacctcaaa caatttgtcc tgggatcggg aaaggtcacc
      121 atcacctaca aagatggcca cagtgaaacc atgaccaagg aaaaactcaa catcacgatt
      181 ggcgccctct acctgcacag atgcctgaca cactacaacc ccaccggaag cattgccctg
      241 gcgctggcca attataactg gtccgatacc aacatgatat attatcacat gccctattat
      301 tttggcatca caacctcacc gttttccaat agacgcgggc aactgcaaac acacacggca
      361 ttggatcgtc aacccctcga gtacattgcc aaatatctgg tcctgcaaca gatgttcaag
      421 gatacgattt tcgctcaagc catcaaaaaa gaagatgaac gcaagcgaaa cctcgtcctc
      481 gccgctctgt aaagggcatc tctaaataac accagccctc acttgacagc acgtcttcca
      541 aaagactaaa taaaccgcaa atccgctttg tacgccatct ccaaaaggga tgacaacttc
      601 acaaaaacac agaaagacac tgatatgaag gatatgaaaa ttaccgattt catcaaaaca
      661 cattaccgcc atttcaatgc cgccactctt gttgacgccg cagaggccta caaacaacac
      721 ctcaaccaag gcaataagat gttggtcaca ctggctggcg ccatgagcac agcagaactg
      781 ggcatcagtc tggccgaaat gatccgtcag gacaaaatac acgccatttg ttgtaccggc
      841 gccaacctcg aggaagacat tttcaacctg gtggcacacg aacactatgt tcgtgtcccc
      901 cattaccgcg acctcactcc tcaggacgaa caaaaattac tcgatcgaca cctcaaccgc
      961 gtaaccgaca cctgtatccc ggaagaagag gccttcagac gactcgaaca caccgtgctt
     1021 gctgaatgga ccggcgctga aaaaaacaac caacgatttt ttccacacga attcatgttc
     1081 cgcatgctca gaagtggcgc tctcaaaaaa tattatcaga tcgatcccaa aaattcctgg
     1141 atgatcgccg cctgcgaaaa aaacctgccc ctctttgtcc ccgggtggga agattccacc
     1201 ttgggcaaca tctttgccgc ccacaacatt gacggttcca tcaaaaaccc gcacgtcgtg
     1261 aaaacaggta ttgaatacat gatggagttg gcccgttggt acacggaaac agccccacaa
     1321 accagcattg gttttttcca gattggtggc ggcatcgcgg gtgacttccc catctgtgtg
     1381 gtgcccatgt tacatcagga ccttcagaga tcgagtgttc cactctgggg ctatttttgc
     1441 cagatcagcg acagcacaac ttcttatggt tcgtattctg gcgctgtccc caacgagaaa
     1501 atcacgtggg gcaaactggg catcgacaca ccaaaattca tcattgaatc agacgccact
     1561 attgtcgcac cattgatgtt cgcctacatc ctggagcaat gataatgaca tcagcatcct
     1621 atgttactgc aacgcgataa gaagcaagac cctatcgatg ccgctaaagt acgcattgaa
     1681 gcagaatcag ttttagaaat ggttttgttg gttatcaaat cggaaacgca aaatcaaacg
     1741 cagagatccc ttcccgaaga aagcagactc aaaggattga ctgaagatct taaaagcgct
     1801 gtgactggtg gggacacctg taaaatgaaa gaatccataa tggccattga tcattttatg
     1861 gcctcttgcg gtgaacagat caagcaagac attgagtctg ctttgccgcg caaaaaaatt
     1921 tcgtgaacct caaagctttt cattacgatc ttcctgaaga actcattgcc aaatacccgt
     1981 gcgaccgtcg tgatgaatcg cgactcctcc ttttggacaa aacaacgggc aaaacaacgc
     2041 acaccatttt caaagacatg gcgactcttt tcaacaaggg ggacgtgctg gtcttcaata
     2101 attcacgtgt gttcccctgc cgactcatca ccaagagaaa aggcgggggg aaacaggaga
     2161 tttttttggt gaggaaggtt gaagacaaaa ctcgtcgatc gtggatcgtg aatcgtggat
     2221 cgagtattaa taatatcgcc atcataaaac gacacacctc agacacgacc tccaaccagt
     2281 ttgccaacca aatttcaaag aacacagatg tcccactaac cgttcgatca gcgattcacg
     2341 attctcgatc cacgatccac gagttttgga ccatcctcat caacgtcagc agaaaagtgc
     2401 gggaaggtga cgtgtttgat tttgatgatt tcacgatcac catttgcgac tctgcgggcg
     2461 ccgaaagact tgctctcatt gagtttgaag gcaagctcat gccgcttttg gaaaaacacg
     2521 ccacagtccc tttgcctccc tacctcaaca gggtcgcaga tgaaactgat cgcaccaggt
     2581 accaaaccat ctacgcctca cagaacggct ccaccgccgc tcccacagcg ggcctgcact
     2641 tcacaccaga attgatggac acactcaagg aacgaggcgt tatcatggcg aacgtcactc
     2701 tgcatgtggg gccgggaaca tttttacccg ttcgcgtcga caacatcgaa aatcacaaaa
     2761 tgcactcag
//
LOCUS       DATDUN010000255         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_97052, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000255 DATDUN010000000
VERSION     DATDUN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97052"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VJC18_03400"
     CDS             <1..709
                     /locus_tag="VJC18_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD44457.1"
                     /translation="LSMVMMARRNMSLKMLKKVARHLSLSKRETDYFAALALYNQADN
                     EQDKDYYFGKMLTLKPLRKMFQLEKDRFAYFTNHQLVVIREMIALPHFEETPEWIAQA
                     LGNSITAQDVKKAISILLRLGLAVRDEKGRLIQSRGALESPVDAEALEIVRYHRQVLG
                     VAKEAITTVPHDKREIISITIPLADAVMPRIKEILRGCRDEIVRCVNHAQEKNFAEVY
                     QVNMQCVPVTKTRKATP"
     gene            706..1872
                     /locus_tag="VJC18_03405"
     CDS             706..1872
                     /locus_tag="VJC18_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44458.1"
                     /translation="MKNIMRIIVVSVICLAALVAGCQPAVDNLMDESTNPFYNEPGVA
                     DVGTVVNNPKMPSSLSYDLFPDFAKYMPESDLLLPQDTFSQASQASSLTAGLVMSFIS
                     KRQIGFELDAIERLGEVVTAALADAGVTIDHTTQSVVLPETEENFMGAFGRLEVKTEA
                     GANEYTRLYFHDRNEDHIIGTALSRLNAEGRTERGVFVMVRPWLWSEGVAGTGARLLA
                     LAYDFSNPEQALLTVREERFHKILQTHMAVEAHFQCQGSLKTCVAEHLEITSPSPERT
                     FGKGAVRMSWTQGSDRYCFASVSYHDGSRDMGDTMLVQGSSLKGLQGLVGETCSFPFV
                     PVWDDRVFTTSDLVMRYEDTDPPQGTAGQYYLDGTSQTGWEGITTQTIKEWLDY"
     gene            2015..>2366
                     /locus_tag="VJC18_03410"
     CDS             2015..>2366
                     /locus_tag="VJC18_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44459.1"
                     /translation="MSNSLSIPIAVLQTNLPTDAVITDADQDGYLNTGDQISANGQTF
                     AVYYDKEYASIPRLERLVGDLNGYLQRVWSDAAPTLSTHSQRMRDEKKISDLVYLDVT
                     DRTGKTAQNFLGTSV"
BASE COUNT          586 a          546 c          646 g          588 t
ORIGIN      
        1 cctgagtatg gtcatgatgg ctcgccgcaa catgagcctg aaaatgttaa aaaaagtggc
       61 ccgacacctg agcttgtcaa aaagggaaac agattatttt gccgcgctgg cgctttataa
      121 tcaggcggac aatgaacagg acaaggatta ttattttgga aaaatgctga cactcaaacc
      181 cctgcgcaag atgtttcaat tggaaaagga tcgttttgcc tatttcacaa atcaccagtt
      241 ggtggtcatt cgcgaaatga ttgccctgcc gcactttgag gagaccccgg agtggatcgc
      301 gcaggcactg ggaaacagca tcacggcgca ggatgtgaaa aaggccatca gcatcttgtt
      361 gcgtctgggg cttgctgtcc gcgatgaaaa ggggcgtttg atccagagcc gcggtgctct
      421 ggaatcccct gttgatgccg aggccctgga aattgtcaga tatcatcgcc aggttttggg
      481 tgtggccaaa gaggcgatca caacggtgcc acacgacaaa cgcgagatca tctccatcac
      541 gattcccttg gcagacgctg tgatgccgcg gatcaaggaa atcctgcgtg gctgccggga
      601 cgagattgtt cggtgcgtaa atcacgccca ggaaaaaaat tttgcggagg tgtatcaggt
      661 caacatgcaa tgtgtgcctg tcaccaaaac aagaaaggcg actccatgaa aaatatcatg
      721 cgcatcattg ttgtgagtgt gatttgcctg gcggcgttgg ttgcaggatg tcagcctgct
      781 gtggacaacc tgatggatga atccacgaat cccttctata atgaacccgg tgtggcggac
      841 gtggggaccg ttgtgaacaa ccccaagatg ccatccagtc tctcttacga tttgtttcct
      901 gattttgcca aatacatgcc tgagtctgat ctgcttcttc cccaggacac attttctcag
      961 gccagccagg cttcgtcatt gactgcgggt ctcgtgatgt cctttatcag taaacggcaa
     1021 atcggatttg aattggatgc catcgaaagg ctgggagagg ttgtgacagc ggctctggct
     1081 gatgcgggcg tgacgattga ccacacaacc caatctgtag tgcttcccga aactgaagaa
     1141 aatttcatgg gtgcctttgg gcgccttgag gtcaagacag aagccggggc caacgaatac
     1201 acgcgtctgt atttccatga ccgtaatgag gatcacatca tcggcacagc cttgtcacgt
     1261 ttgaatgcgg aaggcagaac cgagaggggc gtttttgtca tggtcagacc ctggttatgg
     1321 tcagagggtg tcgccggtac cggtgcgcgc ttgctggctc tggcctatga tttttctaat
     1381 ccggaacagg cgttgctcac cgtgcgtgaa gaaaggtttc acaaaatttt gcaaacacac
     1441 atggccgtag aggctcattt tcagtgccag ggaagcctga aaacctgtgt ggcggagcat
     1501 ctggagatca cgagtccgtc accagaaaga acctttggca agggcgcagt gagaatgtcc
     1561 tggacccagg gcagtgatcg gtattgtttt gcttcggtgt catatcatga tgggtccaga
     1621 gatatgggtg atacgatgtt ggtccaagga tcttcactga aggggttgca gggactggtc
     1681 ggagagacct gttcttttcc ctttgttccg gtttgggacg acagggtgtt tacaaccagt
     1741 gaccttgtca tgcggtacga ggataccgat ccccctcagg ggactgcggg ccaatattat
     1801 ctggacggca cttcacaaac aggctgggaa ggcatcacca cccaaacaat taaggagtgg
     1861 cttgattatt gaggggtttt agaaaatatc agtgccttct ggtcgagttt gtgaccagct
     1921 ttgcctggaa aagttgtttt ttttttgcgt ttttcacgca acatttgcca gcctctgacg
     1981 atatttcctt cagagacaat gaaaggattt tattatgagc aattcattat caatacccat
     2041 tgcggttttg cagactaatc tcccaactga tgctgtgatc accgatgcgg atcaggacgg
     2101 ctatttaaat acgggagatc aaatctcggc aaacggacaa accttcgcgg tttattacga
     2161 taaggaatac gccagcatcc cccgattgga aagattggtt ggtgatctca atgggtattt
     2221 acaacgcgtc tggagcgatg cggcccccac gctttcgacc cattcccaaa ggatgaggga
     2281 cgaaaagaag ataagtgacc tggtttatct ggatgtcaca gatagaaccg gcaagaccgc
     2341 gcagaatttt ttgggcacct ccgtcg
//
LOCUS       DATDUN010000256         1941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_469105, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000256 DATDUN010000000
VERSION     DATDUN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469105"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /gene="atpA"
                     /locus_tag="VJC18_03415"
     CDS             <1..1210
                     /gene="atpA"
                     /locus_tag="VJC18_03415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HLD44460.1"
                     /translation="PAIVGRVIDAMGLPVDGLGPINTTQTRRIEIKAPGIVARRSVHE
                     PLQTGLKAIDSMIPIGRGQRELIIGDRQTGKTAIAIDTIINQKGLGVFCFYVAIGQKQ
                     STVARVVDRLKHHGAMEYTTVIAANASDPAPLQFIAPYSGCTMAEHYRDNGGHALIVY
                     DDLSKQAVAYRQLSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDEHGGGSLTALPI
                     IETQAGDVSAYIPTNVISITDGQIYLETDLFYTGVRPAVNVGLSVSRVGGNAQIKAMK
                     QVAGTLRLELAQFRELAAFAQFGSDLDKTTQKQLARGKRLIETLKQGQYVPMPVSLQI
                     VQIFATTNGFLDDIEVNQVGKYLKDLECYVKANHVSLIKILDEKKALDDEIKNALKEA
                     LTTFTTTFKG"
     gene            1268..>1941
                     /locus_tag="VJC18_03420"
     CDS             1268..>1941
                     /locus_tag="VJC18_03420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HLD44461.1"
                     /translation="MPTLQDIKKRITSIKSTQKITRAMKMVSAAKLRRAQQSLNNHRP
                     FANKLDELVGLILMLSDERQTPALMIPHDHQHNLELLVFTSNRGLCGGFNANLMRHTE
                     SVLKQLKHDQKKTTLQIIGRKGRAFLTARKIPIDHYWPDYADTLTFSQASVLATEAIN
                     RFTQHESDSFSLVYNQFESAASHKIVTKQILPIALSSREDTPDLARQYCFDFIYEPDR
                     ISFLHQL"
BASE COUNT          504 a          596 c          433 g          408 t
ORIGIN      
        1 ccccgccatc gtcggtcgcg tgatcgatgc catgggattg cctgtagacg gactgggacc
       61 catcaacacc acgcaaacgc gccgcatcga aatcaaggcc cccggcatcg tcgccagacg
      121 ctctgtccat gagcctttgc agacaggcct caaggcaatc gactccatga tccccatcgg
      181 ccgcggtcaa cgcgaactca tcatcggcga caggcagacc ggcaaaacgg ccatcgccat
      241 cgacaccatc atcaatcaga aggggctggg cgtgttttgc ttttatgttg cgataggaca
      301 aaaacaatcc acggtggccc gcgtcgtgga caggctcaag caccacggtg ccatggaata
      361 caccaccgta atcgcggcca acgcgtccga cccggccccc ctgcaattca ttgcccccta
      421 ttccggttgc acgatggcgg aacactatcg cgacaacggc gggcacgccc tcattgtgta
      481 cgacgatctg tccaaacagg ctgtcgcata tcgccagttg tccctgctcc tccgccgccc
      541 acccggtcgc gaggcttatc cgggcgacgt tttctacctg cactctcgac tcctggagcg
      601 cgccgccaaa ttaaacgatg aacatggggg agggtctctc actgccctgc ccatcatcga
      661 aacacaagca ggcgatgttt cggcctatat ccccaccaac gtcatttcca tcactgacgg
      721 tcagatttat ctggaaacgg atctttttta cacaggggtc agaccagctg tgaatgttgg
      781 tttgtcagta tcgcgcgttg gcggcaacgc ccagatcaag gccatgaagc aggttgctgg
      841 cacactgagg ctggaacttg cccaattcag agagttggcg gccttcgcgc aatttggatc
      901 cgatctcgac aagaccacac aaaaacaact ggcccgcggg aagagactga tcgaaaccct
      961 caagcaaggg cagtatgtgc ccatgcctgt gagtctgcag atcgtccaga tattcgccac
     1021 cacaaacggt tttctcgatg acatagaggt caaccaggtc ggcaagtatc ttaaagatct
     1081 ggagtgttat gtgaaggcca accacgtttc actcatcaaa attctcgatg agaaaaaagc
     1141 gttggatgac gaaataaaaa acgcgctcaa agaagcgctc acaaccttta caacaacttt
     1201 taaaggatag aatagtgaca cattcttttc gtgaacacgt aaacacgtga acacgtgaac
     1261 acatttaatg cccactctac aagacataaa aaaacgcatc acctcgatta agagcacaca
     1321 aaaaatcacc cgcgccatga aaatggtgtc cgccgccaaa ctccgacgcg cgcagcagtc
     1381 tttgaacaac caccgtccct tcgccaacaa actggatgag cttgttggcc tcatcctgat
     1441 gctgtcagac gaaagacaaa cacccgcctt aatgatccct catgaccatc aacacaatct
     1501 tgaactcctg gtcttcacgt ccaaccgcgg tttgtgtggt ggcttcaacg ccaacctcat
     1561 gcgtcacaca gagtctgtcc tcaaacaact caagcacgac cagaaaaaaa caacgctcca
     1621 gatcatcggc cgcaagggcc gcgcgttttt gaccgccaga aaaatcccga tcgatcacta
     1681 ttggcctgac tatgccgata ccctgacctt ttcccaggcc agcgtcctcg ctaccgaggc
     1741 catcaaccgc ttcacacaac acgaaagcga ttccttctct cttgtttata atcagtttga
     1801 gtccgccgcg tctcacaaga ttgtcaccaa acagatcctt cccattgccc tttcttcacg
     1861 agaggacacg cctgatcttg cccggcaata ttgttttgat tttatctatg aaccagaccg
     1921 tatctcattc ctgcatcagc t
//
LOCUS       DATDUN010000257         3353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_428079, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000257 DATDUN010000000
VERSION     DATDUN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428079"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..482)
                     /locus_tag="VJC18_03425"
     CDS             complement(87..482)
                     /locus_tag="VJC18_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44462.1"
                     /translation="MTKIFGVMMKWVRVLAYYSPLSVQSHALSKTAKNLYTYYPQFIE
                     NIARNFFKVYSQEHGICRRDSFFCIMCCVKDCLLSSFRNREVPLYREAFSVATIVREG
                     RVYDIKVGNFDFAPPPACCKAGVPVGRGW"
     gene            690..>3353
                     /locus_tag="VJC18_03430"
     CDS             690..>3353
                     /locus_tag="VJC18_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HLD44463.1"
                     /translation="MSIRRYILTILFIFAVITSNQCVDPNAAGTGGVASCNPINGVDQ
                     SVDMGYEDFGEYGYETASIPVTIAKNTDGVDATSISFQEASVVPQLTKFVSKDEAGVT
                     QKYATQALGVFFFNRIDNKCGKVLVLNELGMTIKIIDPQILNSHCSYNLVVDGVVIRP
                     NDVYALVATDDDTVVDNIDVSAPVFLKLNPPTALINHYSFNAYITGVGSDVWGNELIN
                     FVDSGVQDLVEVQATPMSFMDIENTETGLVQSSVIFPVLDSNGDSALWSLPAVGGYPY
                     HFVDTQMLSADIVSTPDQMFFFDMNDQIFQLDSDGTLTGLPISSNLEVPDFEIYPSGD
                     YMLYSTRSTNPFTDRESAVLVVYFEETGEHVEAWLEDQQVNFLHFEFEFINHFTVVLC
                     GRTVDDQWVMIRADLSGIIDGFEDTISNWSIIENGQMELWQPRFANDKIYYLCENEET
                     QIINICAMEILDDTTSVAVSLPLSIVSFDISSDLNSYIVFQTANQAKIGGEGPAIGIY
                     NLQTEEISFVTGGQAPTPSNADVDIIAYRHIPELGEGLQVGIINLNNDFVATPADLEI
                     VDNEKYFSLGQHWVLGVRGGRMPYTFEKVSGPGTLNTSIGLFRATDEEGTAIIRVTDA
                     LGDEAYATMHVVGPGKYNVNYNTTGSRDYSPLEGKITAVEVTDNDRYVAAGVSEGDFF
                     VATTEQYGEGESLFDDDGQTTIPVGVGHDAEARDVVMLQGNRVCASGVANSGADDDFA
                     MIKLNIDGDLDGDFGSGGKVLTDFAEGDDGANAMLEQSDGSIILGGYAQVGANVNSAL
                     ARYTADGDLDTTFGPLEDGKVVVAAAPGVLPDQITSMTLLDDDEFLTVGTAFIGGKQR
                     IALMKFNADGTPDLGFGVGGKVITAIGTRDVT"
BASE COUNT          855 a          723 c          873 g          902 t
ORIGIN      
        1 atcttaatgc ttaatgaaca ttaagattat ggaccaaaaa aaagaggaag tcaatcactc
       61 aaaatctttc aaattggtca tgttttctac cacccccgcc ctacgggcac ccccgcctta
      121 caacaggcgg gggggggggc aaaatcaaaa ttacctactt ttatgtcgta caccctcccc
      181 tcccgaacaa tcgtagcgac cgaaaaggcc tcacggtaca ggggaacctc cctgttacga
      241 aaactgctga ggagacaatc tttcacgcaa cacatgatgc agaaaaacga gtccctcctg
      301 caaatcccgt gctcttgaga gtacacttta aaaaaatttc gcgcaatatt ttcgatgaat
      361 tgaggataat aggtgtacaa attcttggct gtcttggaca aagcatgaga ctgtacgctc
      421 agaggagagt aatatgccaa cacgcgaacc cacttcatca tgacaccaaa tatcttggtc
      481 aaaaaaacca cagttgcttg ttgctcagcc ctgttatcga aagtcacctc ttttccccaa
      541 aactgttctt aatctgtaaa tatatttttt tgagagtcaa aaattgccta cctgtctgcg
      601 aaatggtttg ctcacccaac aataacaagg aattagcacc ttgttttttg tgggttttct
      661 ggcacagcga ttgcatcata ttgtcctaga tgagcatcag gagatacatc ttaacgatcc
      721 tttttatttt tgccgtaatt acctcgaatc agtgtgttga tcccaacgcg gcaggcactg
      781 gcggtgtggc ttcttgtaac cccatcaatg gcgtggatca gagcgttgac atgggctacg
      841 aggattttgg ggaatacggc tatgaaaccg caagcatccc ggtgaccatt gccaaaaaca
      901 cagacggcgt ggacgcgacc agcatcagtt ttcaggaagc gagcgtggtt ccccaactca
      961 caaaatttgt ctcgaaagat gaagcaggcg ttacccaaaa atacgccact caggcattgg
     1021 gggtcttttt cttcaatcgg atcgacaaca aatgtggcaa ggttctggtt ctcaatgaac
     1081 tgggaatgac cataaaaatt atcgatcccc aaatattgaa ttctcattgc tcgtacaatc
     1141 ttgtcgtaga tggtgtcgtg atcaggccca atgatgttta tgccctggtg gccacagatg
     1201 atgacaccgt tgttgataac attgatgtga gtgcccccgt ttttcttaaa cttaacccgc
     1261 cgacagcttt aataaatcat tattccttta acgcttacat cacgggcgtg ggttctgatg
     1321 tgtggggcaa tgagttaatt aattttgttg attcaggggt gcaagacttg gttgaggtgc
     1381 aagcaacccc aatgtcgttt atggatattg aaaacacaga gacgggtctt gttcagagct
     1441 ctgtgatttt tcctgttttg gactcaaacg gtgattcggc tctctggtcg ctcccagcag
     1501 tagggggcta cccctatcat tttgttgata cccaaatgtt gagtgccgat attgtgagca
     1561 caccggatca gatgttcttt tttgatatga atgatcaaat atttcaattg gattctgatg
     1621 ggactttaac agggttaccc atctcctcga atttggaagt tccggatttc gaaatatacc
     1681 caagcgggga ttacatgttg tattccacca gatcgacaaa tccgtttact gatagggagt
     1741 cagccgtgct ggttgtgtat tttgaggaga cgggggaaca tgtggaggct tggttggagg
     1801 accaacaggt caactttttg cactttgagt ttgaattcat caaccatttt acagttgttc
     1861 tctgtgggcg aaccgttgac gatcagtggg tcatgatcag ggcggattta tcgggcatca
     1921 tcgacgggtt tgaagacacg attagcaatt ggagcatcat tgaaaatgga caaatggaat
     1981 tgtggcagcc cagatttgcc aatgataaaa tatactactt atgtgagaac gaggaaacgc
     2041 agatcattaa tatttgcgcc atggaaattc tggatgacac aaccagtgtc gcggtgagtt
     2101 tgcctctgtc catcgttagt tttgatattt cctccgattt gaattcgtac attgtttttc
     2161 aaacggccaa ccaggcaaaa ataggcggag agggaccggc gattggtatt tacaacctgc
     2221 aaacagagga aatttcgttt gtgacgggag ggcaagcgcc caccccttcc aatgcagacg
     2281 ttgatatcat cgcttatcgg cacattcctg aattgggaga aggtctccag gttgggatca
     2341 tcaatttgaa caatgatttt gtggccactc ccgcggatct ggaaatcgtt gataatgaaa
     2401 agtacttttc gttgggtcag cattgggtgc tcggcgtgag gggtggtcgc atgccgtaca
     2461 cgttcgaaaa ggtttcggga cccggtactc tcaataccag cattggtttg ttcagggcga
     2521 ccgatgaaga aggtacggcg atcattcgtg tgaccgatgc cttgggcgat gaggcttacg
     2581 ccacgatgca cgttgtcggt cccggaaaat ataatgtgaa ctacaacacg acgggtagtc
     2641 gagactattc accgcttgaa ggcaagatca ctgcagtgga ggtgacggac aatgatcgct
     2701 atgttgcggc cggtgtctcc gagggggatt tctttgttgc caccaccgaa cagtatggag
     2761 agggcgagtc attgttcgat gatgacggtc agacgaccat accggttgga gtgggccacg
     2821 atgccgaggc gagggatgtt gtgatgttgc aaggcaatcg tgtctgcgcc tcgggtgtgg
     2881 ccaattctgg ggccgacgat gattttgcca tgatcaaatt gaatattgat ggcgacctgg
     2941 atggagattt tggctcaggc ggaaaggtgt taaccgattt tgctgagggt gatgatgggg
     3001 ccaacgccat gcttgagcaa agcgatggga gcatcatttt gggcgggtat gctcaagtgg
     3061 gtgccaatgt gaattctgcc ttggctcgtt acacggcgga cggtgatctg gataccactt
     3121 tcggacccct tgaggatggg aaggttgtcg tggccgcggc acctggcgtg ctccctgatc
     3181 agatcaccag catgacactg ttggatgatg atgagttttt aaccgtaggg acggccttta
     3241 ttggcggtaa acagaggatt gccctgatga agtttaatgc cgatgggacg cctgacctgg
     3301 gctttggtgt gggtggaaag gtgatcaccg cgatcggcac tcgagatgtg acg
//
LOCUS       DATDUN010000258         1232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_552009, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000258 DATDUN010000000
VERSION     DATDUN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552009"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VJC18_03435"
     CDS             complement(<1..508)
                     /locus_tag="VJC18_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44464.1"
                     /translation="MTGMATNYLSPVQSNILGQLAHGNVGALGNANPEQLAGVINAVV
                     NHGASAIGVREAFTGYTERADATTSATLVQTAQPFAARILKQQKAAVLADVREQNASR
                     PAGEPIVLTQMMLGAMISAKAPATQRYAKDAPVLGYVIRGGYADIPEAIRNLMGNIDR
                     TTYSDEWFQ"
     gene            739..1191
                     /locus_tag="VJC18_03440"
     CDS             739..1191
                     /locus_tag="VJC18_03440"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLD44465.1"
                     /translation="MDKNKARLFSIIISILAGMGLAIMMQINATLGQKVGLLESSFIV
                     HLAGMTLAIVLLNTKLNKDFIVRAKTAPKIYFLAGVFGVLMVIVANYLVPKIGMAVCV
                     SLFVTTALVLTTLVDHIGIFNLARFKITPKRVAGLVLAIGGVVMLIWG"
BASE COUNT          300 a          300 c          302 g          330 t
ORIGIN      
        1 gttggaacca ctcatcagag tacgtcgtac ggtcgatatt acccatgaga ttacgaatcg
       61 cttcggggat gtctgcatag ccacccctga tgacgtaacc aagcaccggc gcatccttgg
      121 catagcgttg tgtggcggga gctttggccg agatcatggc acccagcatc atttgagtga
      181 gcacaatggg ttctcctgca ggacgtgagg cattttgttc cctgacatct gccaacacgg
      241 cggccttttg ctgtttcagg atgcgagcgg caaaaggttg ggccgtttgc acaagtgtag
      301 cagacgttgt tgcgtctgca cgctctgtat aaccggtaaa tgcttcgcgc acgccaatgg
      361 ctgacgcgcc atgattgaca acggcattga tcacaccagc caactgctcc ggatttgcat
      421 tacccaaagc gcccacgtta ccatgggcca actgccccag aatatttgat tgcactgggg
      481 ataaataatt tgttgccata cctgtcatga tgatctcctg tttgattttt tgtcaccgct
      541 cacctcagac atgaccatca tgaccaagca aagcggtagt ctaaaacttg taaaatctag
      601 cttccctgag tacctctatt atcggtaagc ctcaagcaat gttgctacct ttcgtcatta
      661 aaccctgaat tccgtcattt gcgaacaaac ttctcattgc gaaaagatga aatatgaata
      721 atgacttaaa gcattaaaat ggacaaaaat aaggcgcgcc ttttctcaat catcatcagc
      781 attctggccg gcatgggtct ggccatcatg atgcagatta acgccacgct gggccagaag
      841 gttgggcttc tggaatcgtc tttcattgtg catctggcgg ggatgacgct cgccattgtt
      901 ttgctcaaca caaaactcaa caaggatttt attgtgcgtg ccaaaaccgc acccaaaatt
      961 tattttttgg ctggtgtgtt tggtgtgctc atggtgatcg tggccaatta tctggtgccc
     1021 aaaatcggca tggcggtctg tgtgagcctt tttgtcacca cggcactggt gctcaccacc
     1081 ctggtggatc acatcggcat tttcaatctc gcccgcttca aaatcacacc caaaagggtc
     1141 gctggactgg tgttggctat tggtggggtg gtgatgttga tttgggggta agaaatatcg
     1201 aatatcgaat atcgaacaag gaatgtcgaa gg
//
LOCUS       DATDUN010000259         3580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_302963, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000259 DATDUN010000000
VERSION     DATDUN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302963"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..476
                     /locus_tag="VJC18_03445"
                     /pseudo
     CDS             279..476
                     /locus_tag="VJC18_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
     gene            complement(551..781)
                     /locus_tag="VJC18_03450"
                     /pseudo
     CDS             complement(551..781)
                     /locus_tag="VJC18_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(777..2543)
                     /locus_tag="VJC18_03455"
     CDS             complement(777..2543)
                     /locus_tag="VJC18_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HLD44466.1"
                     /translation="MNKVFFVRIRVLFCLLFVFSTMLLYSGTVWAVTFFVNDLNDTVD
                     IVPGNGVCADASGHCTLRAAVIETNALAGADKISLMVSGTYFLNIAGSGVADEDLAQN
                     GDLDIADDLEIQELFFVSATIDGNGTERIFELVSPELIFKIKSIQLQNGSASFTGTSG
                     GGMLLDEAGNNSITLQSVTVIDNYSRNRGGAVYTTMPDTTLNIINSSFQRNEANTSGG
                     ALYMYDSSFSHLCRVLVDSSEFLENTSGYDGGAIYISGCAQVTLQNSVTISANQATND
                     AGGLRLCDNTSVNLSNTLIDSNSSGSYAGGLYSIWNTSISVTRSNITNNESGVNTGGA
                     FFLYDTNVSLQHTSIVNNVAANRGGGLYFYGVTATIDSALIEQNSSDDVAGVYIGYSN
                     VAIEDSSIINNTITDALLSDISGLSTTESTVQIDNTTFSGNTGPVTLGVLPFSSGADT
                     SHVVLHSVTIARNNGLGIGNTYSSTATIEATNSLIAQNTGFLVSGLDCYGGVVSNGYN
                     LVGATDSSCTGFSDPTDLVGSTTVPINPYLRSLGLYGGSTPSHALASTSPANGASNDC
                     LATDQRGFARPSTGCSIGSFEY"
     gene            3215..>3580
                     /locus_tag="VJC18_03460"
     CDS             3215..>3580
                     /locus_tag="VJC18_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44467.1"
                     /translation="MTRYLKELAELFTKHKIKVHFLPEGETCYCRRNDSEGGQRETSP
                     AKEHGIFLCRGASLSSALHEMTHAIPSQEVLPLLEEMFPGFDRDLLAFPPLPGRSDNQ
                     TEVFTKLAGRYFRDEAYSGI"
BASE COUNT         1020 a          878 c          786 g          896 t
ORIGIN      
        1 ttgacgattt ggtgaaaaac tgtattttga gacataaagt ccgatcctcc gtggaaagtg
       61 ttcgcgagtg tggtaacccg caaatgccca cagaatcgga ctttatcaat ttatagattt
      121 atttaaccgg acaggcgtga attcaatata ttataattat caacagtttt tccgagttat
      181 gcctcggaaa ggttgataac aaaaaataat gtgcagacgc atcatacgaa caaaggacat
      241 gatactgccc agagtatgtg gtaccgttct accaaaggat gcaatcaatc ggggatttcc
      301 gcagggggtc tatttcagtt accttctgca aatgtggcaa aaaaaattgt gcttgcgccg
      361 ccaaagatca tccgggccat gggccacatt accgttggac aacgacgcgg aagggtaaaa
      421 gttttgcgca acacctgcct ttgggaccag aactggacaa agcacgaaaa gaaattggtg
      481 cttacatatt acaacgcaac caacggcaat ggtataattc ccaaagacaa atcgttgaag
      541 atcaaggcgc cagcagttca tcgagattct gtttttcaat gcgacagcgc cacttcacca
      601 tcggcgttgg ttcaatcgga aactgatgtt gaaaatcccg ctccccgcaa aagtattgcc
      661 aatatggatt ctcaacccac ttctcgacaa cagactcgtc tgattcgtca tagattgctt
      721 tcagataatg caatccaatc atacagataa gcctcgtggt tttgataccg ggccgcttaa
      781 tattcaaaag atcctatgct acaacccgtg gagggtcttg caaaaccccg ttgatcagtt
      841 gccaagcagt cgttgcttgc cccgtttgcg gggctggtgg aagccaaggc atggctgggc
      901 gtggagccac catacagacc aagcgacctg agatacggat tgatagggac tgttgttgat
      961 cccaccagat cagtggggtc gctaaagccg gtacaagatg aatcagttgc ccccacaaga
     1021 ttataaccat tggaaacaac ccccccataa caatcgaggc cagacaccaa aaagcctgtg
     1081 ttttgagcta tcaatgagtt ggttgcctca atggttgcgg ttgaagaata ggtgttgcca
     1141 atccccaagc cattatttcg agcgatagtc acgctatgca agacgacatg actggtatcg
     1201 gccccagacg aaaacgggag cacacccagt gttacgggcc ccgtgttgcc agaaaaggtt
     1261 gtgttgtcga tctgtaccgt tgactctgtt gtgctaagcc cgctgatgtc agaaagaagg
     1321 gcgtctgtta tggtgttatt gataatactg gaatcctcaa tagcaacatt gctgtaacca
     1381 atgtaaacac cagcaacatc atctgatgag ttttgttcaa tgagggcact gtctatagtg
     1441 gctgtgactc cataaaaata aagccctcct cccctgtttg ccgcgacatt gttgacaatg
     1501 cttgtgtgtt gcaggctgac attcgtatca tacaagaaaa aggccccccc agtattcact
     1561 ccagattcat tgttggtgat gttggagcgc gtaacactga tgctggtatt ccatatggaa
     1621 tacagaccgc cagcataact cccgctactg ttcgaatcga taagggtgtt tgacaaatta
     1681 actgaggtat tgtcacacaa acgcaggcca ccagcatcgt ttgtggcttg attggcactg
     1741 atggtgactg aattttgcaa cgtcacctga gcacacccac taatgtaaat ggctcctcca
     1801 tcgtatcccg aagtgttttc gaggaattcg gaactgtcaa cgagcacccg acataaatgt
     1861 gaaaaactcg aatcatacat gtagagtgcg ccgccgctag tgttggcctc gtttctctga
     1921 aaagacgaat tgataatatt gagcgtggta tcaggcattg tggtataaac agccccacca
     1981 cgatttcttg agtagttatc aatgacggtt acggattgaa gggtgatgct attgttgcca
     2041 gcctcatcca atagcatgcc gccaccactg gttcctgtaa aagaggccga accattttgt
     2101 aattgaatac ttttgatttt gaaaattaat tcagggctta cgagttcaaa aatgcgttcc
     2161 gttccattcc catcaatggt tgctgaaaca aaaaagagct cctgaatttc cagatcatca
     2221 gcaatgtcca gatcgccatt ttgggccaaa tcttcatcgg cgacaccgct accggcgatg
     2281 tttagaaaat aggttcccga gaccattaga cttattttgt ccgccccggc caacgcattt
     2341 gtttcaatca cagcggctcg caaagtacaa tgacccgagg catctgcgca aaccccattg
     2401 cccgggacta tgtcaacagt gtcattcaga tcatttacaa aaaaagtaac tgcccagact
     2461 gttcccgaat acagaagcat ggttgaaaaa acaaataaca gacaaaataa aacccgtatt
     2521 ctaacaaaaa aaactttgtt cataattgct ccaagtggat gacactggct acgggctatg
     2581 atgctccgtt gctgtgttga aaggcctatt atgggaaatt catgaaaaaa gtaaaacgaa
     2641 ttgatctctt agtgtccaca gatgtgtgta cacagtcacc gttcaaagtg tccacaactg
     2701 tggactgaat tttcacgtcc aacacaactt ttcattttgc ccgccgagaa cgttgcatga
     2761 aactcaggga atagtaagtc tattgtatta gcccatgata cgagtgtcta caatactgcc
     2821 ctcgcaaata tcctcttacc ggccctgcgc aagaggcccc tgcgcaccaa tccgcgcgaa
     2881 tgacctcata aaagcaacac cacgttcttg ttccaaaaga gaatcacctg cccaaaccca
     2941 ctcggataca aacgaccctg cccggaagtt ggccagactc gttcaggctt taccaatcgc
     3001 gctgacaggt tcctcaaaag tgtttgcccc tgaggatcag ttgatacaga ctcagtcacc
     3061 aaccacaaca ttgccagtca tacaggaaca atttggcccg cttttctcac agggcatcca
     3121 atcacatgca gtctttgaat tgttcttgac catgcttcaa agactcgatg atttggattg
     3181 cagacatata gaccaaccgc cagacactca atcattgacc cgatatttaa aagaactggc
     3241 tgaacttttt acaaaacata aaatcaaggt gcatttttta ccagaagggg aaacctgtta
     3301 ttgccgcaga aatgactcgg aaggcgggca acgtgagacc tctcccgcta aagaacacgg
     3361 aatttttctt tgtcgcgggg catccctgtc atccgcatta cacgagatga ctcacgccat
     3421 tccctcgcag gaagtgttgc ccctcctcga agagatgttc cccggtttcg atcgcgacct
     3481 gctggcattc cctcctttgc ccggcagaag cgacaatcaa accgaagtgt tcacaaaact
     3541 cgccggtcgc tatttcaggg acgaggccta ttcaggcatt
//
LOCUS       DATDUN010000260         4998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_303055, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000260 DATDUN010000000
VERSION     DATDUN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303055"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..2263)
                     /locus_tag="VJC18_03465"
     CDS             complement(260..2263)
                     /locus_tag="VJC18_03465"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HLD44468.1"
                     /translation="MKRIEVVPVATAAERGLWNEFMVRWHYLGKTGLVGSQRRYLISS
                     ERGYLGGLSFSSAAWAVQDRDAWIGWSSQARQAHLNHIVSNSRFLILPWVHVPHLASH
                     VLSVAIKRLEGDWQETYGYKPVLVETFVERERFVGTCYRAAGWTEVGETTGRGRQDKN
                     RTATIPVKKIFLKPLREDFRDVLCVEPVGTAPLFSDWAYEEFARASLGDKRLKQRLIT
                     IAQDFYARPDGNIPKSSGSRAKTKATYRFLDHGDVTMKKILEPHYEATARRCNREKVV
                     LAIQDTTSFNYQTHPDTEGLGLISRHANGAVGILLHETLTVNPQGVSLGLLDAQSWVR
                     DPADYGKKERRRKLPTEEKESYKWLKSFKAVAKFQKRLTQTTLVSVGDREADVYELFQ
                     FAKSRPEHPKLLVRAARNRALSDDQKYLWEYVEAQPVAGVQELQVPRRKGQKARLARL
                     EIRHSEVSLKPPRHRNCRTGLRTITVHAILAKETDTPEGQIPLEWMLLTTLEVKNLDG
                     AIEKLKWYSLRWQIEVYHKVLKTGINIEQRQLGNRDRIENCLAIDLVVAWRVLHLTKL
                     GRETPDVPCTVVFEDFEWKALFVFVNRTREIPTTPPTLREAMRAVAGLGGFLGRKGDG
                     EPGPQTLWVGLQRLDDIATAWQIFVATMLPQKELPVPSKSDYG"
     gene            complement(2695..3147)
                     /locus_tag="VJC18_03470"
     CDS             complement(2695..3147)
                     /locus_tag="VJC18_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HLD44469.1"
                     /translation="MLLNREFQKLLQCLNREKVKYLIVGAYAVMFYAEPRFTQDIDIW
                     VKADRTNAHKILRALKHFGLPVEDITVDDLTNPELMYQIGKEPCRIDILLGIEGVTFE
                     ESWKNRKSAGLKENRVSFIGLNELIKNKKKSKRLQDIIDLKKLRYVAR"
     gene            complement(3429..3869)
                     /locus_tag="VJC18_03475"
     CDS             complement(3429..3869)
                     /locus_tag="VJC18_03475"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLD44470.1"
                     /translation="MHLTPHTYPIEHKTISKDRFSFVLRHYDLPTLMAGKMLACLDRV
                     WEKRGVKVKGRDYYDLLWYMQKQIVPNSDRLAKSMHACTIGEAFGKIRQKVKAIKPRD
                     LLTDLRPLFENGDFAQKWVKTFREQFEHLYGSYVAELRIDHCAD"
     gene            complement(3938..>4998)
                     /locus_tag="VJC18_03480"
     CDS             complement(3938..>4998)
                     /locus_tag="VJC18_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44471.1"
                     /translation="TDRYNLLPRLGDVSGLGRGASKDPLNITHDSVFRYDLQDRATSA
                     HAHARLCRRLGTKLSLRGPSVFNPFGGCDVHTPFMLVDSAVDVFQQGAESGGCLNDIY
                     LWRQMSETNRTACVYQHGFDMTQFYGSLTERYGLRGLMALAMARIEILLKGSIVFVSF
                     FDIEGNSPQIRFLASEDIQAETISPHYLIAFTAGDSDTIKRYWYFQHELMRPNGFEKL
                     ARQLPVDTLILKGVPGLFRGRTRSRALRLALTAARRSDASVLTDVQSKRSGHEFEESG
                     QPYNIWRAGYRPEEIAFDEVSSFGYSISSTKSPIYSGMGTHLIEEGAMMMSLGRSVWE
                     SAKECLVDLRLVIGGSNI"
BASE COUNT         1289 a         1332 c          986 g         1391 t
ORIGIN      
        1 ttttccgcat ttgccaaatg ttgccgagat agatcccctg cgaaaatcac caaaggagag
       61 caccttttga taaagacgtt ctcgttgaat ttcgagtgct tttagatcat ctgacataga
      121 cacctctttc ttaatgttta accaacatta agattatgag gcaaaaaaaa gaggaagtca
      181 atctcgaaaa atctttcaaa ttgaccatgt tttctacctc ccccagcctt cggctacccc
      241 ctccaaatca ttctgatctt tacccataat ctgacttgct gggcacaggg agttcttttt
      301 ggggcaacat cgtcgcaaca aaaatttgcc aggccgtagc aatatcatcc aatcgttgta
      361 aacctaccca caaggtttgg gggccgggct caccatcacc cttccgcccc agaaaaccac
      421 cgagtccagc tacggctctc atggcttctc gaagtgttgg tggtgttgtt ggtatttccc
      481 gtgtgcggtt cacaaaaaca aacagtgcct tccattcaaa atcctcaaac acaacggtgc
      541 atggcacatc cggcgtttcg cgtccgagtt tcgtcaaatg cagaacccgc caagccacca
      601 ccaaatcaat tgccaaacaa ttctctatcc gatccctgtt acccaattgc ctttgctcta
      661 tgttgatccc tgtctttaat actttgtgat acacttcaat ttgccaacgc aaactgtacc
      721 acttgagttt ttcaatcgcc ccatcaagat ttttgacctc aagtgtcgtc aacaacatcc
      781 actcaagtgg gatttgtcct tccggtgtgt ccgtttcttt tgccaaaatg gcatgcactg
      841 taattgtgcg tagccccgtc cggcaatttc gatggcgggg tggtttcaaa ctcacttcag
      901 aatgccgaat ctcaagacga gccaagcggg ccttttgtcc cttgcgtcgt ggaacctgta
      961 attcctgaac ccctgccaca ggctgcgctt cgacatattc ccacaaatat ttctgatcat
     1021 cggacaacgc ccgattgcgt gctgctctta ccaacaattt ggggtgttca ggccgagatt
     1081 ttgcaaattg aaataattcg taaacatctg cctcacgatc acccacggag actaatgtcg
     1141 tttgcgttaa cctcttctga aattttgcta ctgccttaaa actcttgagc cacttgtaac
     1201 tctccttttc ttcggtgggc agtttccgcc ggcgttcttt tttcccgtaa tccgccggat
     1261 ctctcaccca gctttgtgca tccaacaacc caagggacac tccctgcgga tttaccgtca
     1321 aggtctcatg taacaagatc ccaactgccc cgtttgcatg acggcttatc aacccaagcc
     1381 cttctgtgtc agggtgcgtt tggtaattga atgacgttgt atcttgaatc gccaaaacaa
     1441 ccttctcccg attacatcgg cgagctgttg cttcataatg aggttctaat atctttttca
     1501 ttgtcacatc accatgatcc aaaaaccggt acgttgcttt ggtcttggca cgacttccac
     1561 tcgattttgg gatgtttccg tctggccgag cgtaaaaatc ttgtgctatc gtaatcagtc
     1621 gctgctttaa ccgtttgtct cccagagacg ccctggcaaa ctcttcatag gcccagtcag
     1681 agaaaagagg agctgtaccc acaggctcca cgcataaaac atctctgaaa tcttctctca
     1741 aaggttttaa aaaaatcttc tttaccggaa tcgtcgcggt gcgattttta tcctgtctgc
     1801 cgcgtcccgt agtctcacca acctccgtcc agccagctgc cctataacaa gtccccacaa
     1861 acctctcccg ttccacaaat gtttccacaa gtacgggctt atatccataa gtctcctgcc
     1921 aatctccttc aagtcgtttg atcgccaccg acaaaacgtg tgaagccaaa tgtggcacgt
     1981 gtacccaggg taatatcaaa aaacgggaat ttgatactat gtgattcaaa tgcgcttggc
     2041 gagcctgaga actccagcct atccacgcat ccctgtcttg taccgcccag gctgcagacg
     2101 aaaaactcag accaccaaga tagcctcttt cggaagatat gagatatcgc ctctgagacc
     2161 ctacaagacc tgtctttccc agataatgcc agcggaccat gaactcattc cacaaacctc
     2221 tttctgctgc cgttgcgacc ggcacgactt caatccgttt caactcattc aaattacaca
     2281 ataccggaac agtggacaca gacgaaactt ccggcaactt ataagcagga atagtgggac
     2341 ccgcttgagg caaatgaatt aaaccttgcc ggctgagctt attcagggcc ttccgacaac
     2401 ccacctcccg aggccgtcca tcggaagtct tccagtttac ccattcacaa accagtctcg
     2461 atagtctgct tcgcgataca agagcagact cctgttgaat ccggattatg atctctgagg
     2521 tgaaccgttt gccctgtatg gttaactccg tttccaccat atctgtctac cacagtattt
     2581 tgtctgtgtc cagcaatttg agttatggga caggttcaga tctggcgtgt tgcatgtccc
     2641 tagtcaagac cccaatgatg ttcaggggga cacgattgac cccatgatct ggaattatcg
     2701 ggctacatat cgtaattttt tcagatcaat gatatcctga aggcgtttgc tctttttttt
     2761 gtttttaatc agctcattta atccgataaa tgaaacacga ttttccttta atccagcaga
     2821 tttcctgttt ttccaggatt cctcgaaagt gacaccctca atgcccaaca gaatatcaat
     2881 acggcatggt tccttgccaa tttgatacat cagttcagga ttggttaaat catcaacagt
     2941 aatatcctcc accggtaagc caaaatgttt taaagctctc aatatcttgt gcgcattggt
     3001 acgatctgct ttgacccaga tatcaatatc ctgtgtaaaa cgcggttcag cataaaacat
     3061 caccgcataa gcgcccacaa tgaggtactt aactttttct ctgtttaagc actgtaatag
     3121 tttttgaaat tctctgttga gcaacattta ttttagatct ccacaaataa taatcgcaca
     3181 ccattttcca ggcagctttg aatttgactt ctggccccag agcattccaa aaatcaatcg
     3241 aaaaagaatc atctgctttt tttactggga ccaatcttgt catgatgagt ttgggggatc
     3301 tgatcttgtg gttattattt gttttaaatg gtttcatccc tgtcttgtcg atacattgac
     3361 atttacggca ctgtcctcaa cctaaagtat cagtgtggcg ctcaggctaa cagatcgata
     3421 tgataaaatc aatcagcgca gtggtcaatt ctcaattccg ccacataact cccgtacaaa
     3481 tgttcaaatt gttctcgaaa tgttttgacc cacttttggg caaagtcgcc gttttcaaat
     3541 agggggcgca aatcagtcag cagatcacgg ggtttaatgg ccttcacttt ttgtctgatt
     3601 ttcccaaaag cctcgccgat ggtgcaggcg tgcattgact ttgccaggcg atcactattg
     3661 ggaacaatct gcttctgcat gtaccagagc agatcataat aatctctgcc tttcaccttt
     3721 accccgcgtt tttcccagac acggtccaga cacgccagca tttttcccgc catgagagtg
     3781 ggcaggtcat aatgccgcag aacaaaactg aatcgatcct tcgagattgt cttgtgttcg
     3841 atcggataag tgtgtggggt caggtgcacc tcaaccaata aaatcaggtg ccaggtacat
     3901 ttttgcagac aatgttattc ttgattttgt aaagatttca tatgtttgag ccaccaataa
     3961 ccagccgcag gtcaacaaga cattctttgg cggattccca cacagacctt cccaaactca
     4021 tcatcattgc cccctcttca atcaaatgag tgcccattcc agagtaaatt ggggattttg
     4081 tggatgatat cgagtaaccg aaactggata cctcatcgaa ggcaatttct tcaggacgat
     4141 acccagcacg ccatatatta tacggttgcc cactctcctc aaattcatgt ccacttcttt
     4201 tggactgcac atctgtcaac acggaggcat cgctacgccg cgccgctgtc agtgccagtc
     4261 tcagggcccg cgatctggtt ctgcctctga agagtccggg cactcccttg agtatcaatg
     4321 tgtcaacagg caattgccta gccagttttt caaaaccatt gggacgcatg agctcgtgtt
     4381 gaaaatacca ataccgcttt attgtgtctg agtcccccgc tgtgaacgcg atcaagtaat
     4441 ggggagaaat tgtttctgcc tgaatatcct ccgacgcgag aaatctgatc tgcggggaat
     4501 tgccctcgat gtcaaaaaaa gacacaaata cgatactgcc ttttaataat atctctatcc
     4561 ttgccatagc caaggccatc aaacccctga gtccataacg ttcagtcaaa ctgccataga
     4621 actgggtcat gtcaaaacca tgttgataga cacaagctgt cctgttggtt tcggacattt
     4681 gccgccacaa ataaatatca ttcagacaac caccagactc tgccccctgt tgaaacacgt
     4741 ccacggctga atccaccagc ataaagggag tgtgtacatc acaaccccca aagggattga
     4801 acaccgaagg gcctctcaat gacagcttag tccccaatct gcggcaaagc ctggcgtggg
     4861 cgtgggcgga tgtcgctctg tcctgcagat cataacggaa tactgaatca tgtgtaatat
     4921 tcaaaggatc ttttgatgcg cccctcccca atcccgacac atctcccaac cgtggtaaca
     4981 aattgtatct gtcggtgt
//
LOCUS       DATDUN010000261         2670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_303056, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000261 DATDUN010000000
VERSION     DATDUN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303056"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VJC18_03485"
     CDS             complement(<1..842)
                     /locus_tag="VJC18_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44472.1"
                     /translation="MTIGHNKIRKTFLELAQSHKLPQTMLFYGLAGIGKKRIALDLVS
                     HLFCANPPAQHSSPQACGVCQACLMLGQNNHPDCFLIAPTPPKSQGDKPVNATSLDVQ
                     PNWTIKTDQFKALRNKLIYHPLMAKHQVVLIDDAEKMTTATANSLLKILEEPRPHQIF
                     ILITAQTNKILPTIRSRCAKYYFSPLTDQEAKQIIVAQAEDTSLPDEATLSFLVRCFQ
                     GSITNTLKALQSSDLLDFLRTLTAPALSFRQLAAHSKKLATSDQDLNIFLQCLNHYCL
                     EQQK"
     gene            complement(897..1556)
                     /gene="tmk"
                     /locus_tag="VJC18_03490"
     CDS             complement(897..1556)
                     /gene="tmk"
                     /locus_tag="VJC18_03490"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HLD44473.1"
                     /translation="MSTYFGLMFITFEGTEGCGKSTQIKRLKLFLEKQGREIVLTREP
                     GGTVIADKIRKILVDASNHNMVPLCEVLLYYASRAQHIHELIKPALQQGKIVLCDRYN
                     DSSLAYQGTARGMDQGLLEQLSQLVVNQHKPDLTFLLDLPVETGLKRARDRAHTLHPE
                     EREDRFENEVIEFHRAVRCGFLELAKKEPARFCVINADRDEDSIFEDITRIVNDKLLV
                     T"
     gene            complement(1679..>2670)
                     /locus_tag="VJC18_03495"
     CDS             complement(1679..>2670)
                     /locus_tag="VJC18_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLD44474.1"
                     /translation="TGTRGAGGITQGDLRFAPTASSKHFGFYVDQAEIDVESEFGENI
                     RARVDFDLRDLGSPSVSAFLLEQAYVTANFPIGNGMEFLIGKFNAPIGTESVDRHENI
                     FPTYTPGYVFLVPKQVMGSKIYYDFNDNWNFDFVLVDSLNATLGGNSPYPSGIMRFGV
                     IWGDEGRESYVHLAGGFGPEHNVAVGQSSEDMHFDMLGDLWANFAFGDFWDLGFEATY
                     RMTDTRIGTTNQTAMAGQLFTKYQASEVWSVQLRAAAFWDMDTAGLGSGASTTGGTWG
                     GFEGKTYSGSLATTYQITDGANFTFEYRFDYADPVGLATANFHTVMTEFAYNF"
BASE COUNT          647 a          620 c          724 g          679 t
ORIGIN      
        1 tgtttttgct gttccaaaca gtagtggttc agacattgca gaaaaatatt caagtcctga
       61 tcgctcgtgg cgagtttttt tgagtgcgcg gcgagttggc gaaaagaaag agcaggtgcc
      121 gtgagggtac gcaggaagtc aaggaggtct gatgattgaa gggccttcag tgtgttggtg
      181 atggacccct gaaaacaccg cacgagaaaa gacagcgtgg cttcatcagg caaagaggtg
      241 tcctcggctt gtgcgacaat aatttgtttt gcttcctggt cggtgagggg tgaaaaataa
      301 tatttcgcgc aacgcgacct gatggtgggc agaattttgt tggtttgagc tgtgatgaga
      361 atgaaaatct ggtgcggtct gggttcttcc agaattttta gcagagaatt ggctgtggct
      421 gtggtcattt tctcggcatc gtcaatgagc acaacctgat gtttggccat gagcggatgg
      481 tatatgagtt tgttgcgcag ggccttgaac tgatccgttt tgatggtcca attgggttgc
      541 acatcgaggc ttgtggcgtt tacgggtttg tccccctgtg atttgggggg tgtgggtgcg
      601 atcaggaaac agtcaggatg attattttga cccagcatga gacaggcctg acaaacgcca
      661 caagcctggg gacttgagtg ttgtgcaggg ggattggcac aaaacaaatg ggacacaaga
      721 tcgagagcga ttcttttttt gccgatgcca gcaaggccgt agaatagcat ggtctgcggt
      781 agcttgtggc tttgcgccag ttcaagaaaa gttttgcgaa ttttattgtg tccgatggtc
      841 atgagaaaat ggcttaagcc ccaaggcgta aggctcaggg cgcaggcaag cgtaagtcag
      901 gttacaagca atttgtcatt aacgattctg gtgatatcct caaaaatact gtcttcatca
      961 cggtctgcgt tgatcacaca gaatcgtgcg ggttcctttt ttgccaattc aaggaaaccg
     1021 catcgtaccg cccggtgaaa ttcaatcacc tcattttcaa atcgatcttc ccgttcctct
     1081 ggatgcaacg tgtgagccct gtcgcgggcc ctctttagac cggtttcgac aggcagatcc
     1141 agcaaaaagg tcaaatccgg tttgtgctgg ttgaccacaa gttgactgag ttgctccaac
     1201 aaaccttgat ccattccacg cgccgtaccc tgataggcca ggctggaatc gttatagcgg
     1261 tcacagagga caatcttgcc ctgttgcaat gcgggtttga tgagttcgtg aatatgctgt
     1321 gctcgtgaag cgtagtagag aagtacctcg caaaggggga ccatgttatg attgctggca
     1381 tcgaccagaa tttttctgat cttgtcggcg atcacggttc cccccggttc tcgtgtgagc
     1441 acaatttcac gcccctgttt ttccaaaaac agtttcaacc gtttgatctg tgtggacttg
     1501 ccacagccct ctgtgccctc aaaagtgatg aacataagcc caaaatacgt gctcacgtat
     1561 tcgtgtgttt acgtgttcac gaaaaaggcg taaggcttaa ggcgcagggc aaaaggaaaa
     1621 atccttacgc cttacgtctt aaggccttac gcccaagatt ttcaatgtac gcacgaactc
     1681 aaaaattata cgcaaactcg gtcatgacgg tgtggaaatt ggcagtggcc aaccctacgg
     1741 gatcggcata atcaaagcgg tattcaaatg taaaattggc accatcagtg atttgataag
     1801 tggtcgccaa gctaccgctg taggttttgc cttcaaaacc accccaggtt ccgcccgttg
     1861 tagacgcccc cgagcctaga ccagcggtgt ccatgtccca gaaagccgcc gcgcggagtt
     1921 ggacggacca gacctctgat gcctgatact tggtgaaaag ttgtccagcc attgccgttt
     1981 ggttggttgt gccgatccgt gtgtcggtca tgcgataggt ggcttcaaat cccaaatccc
     2041 aaaaatcgcc aaaggcaaaa ttggcccaga ggtcgcccag catgtcgaaa tgcatgtctt
     2101 ctgaactttg gccaacggcc acattgtgct cggggccaaa cccgccagcc aggtgaacgt
     2161 acgattcacg tccttcgtct ccccaaatga cgccgaacct cataatgccc gacgggtaag
     2221 gactgttgcc tcccaaggtg gcgtttaaac tgtcgaccaa aacaaagtcg aaattccaat
     2281 tgtcgttgaa gtcgtaatat atcttggaac ccatgacctg tttgggcacg aggaacacat
     2341 aaccgggggt gtatgtggga aaaatatttt cgtggcggtc cacgctttcg gtaccaatgg
     2401 gggcattgaa tttaccaatg aggaattcca tgccatttcc gatgggaaag ttggcggtga
     2461 cgtaggcttg ttcaaggaga aaagcgctga cgctgggtga accgaggtcc ctcaaatcga
     2521 aatccacgcg ggcacggatg ttttcgccaa attcactttc cacatcgatc tcggcttggt
     2581 ccacataaaa tccaaagtgt ttggaacttg cggtgggcgc aaagcgcagg tcgccctggg
     2641 tgattccacc ggcgcctctg gtgcctgtga
//
LOCUS       DATDUN010000262         5164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_347388, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000262 DATDUN010000000
VERSION     DATDUN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347388"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VJC18_03500"
     CDS             complement(<1..184)
                     /locus_tag="VJC18_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HLD44475.1"
                     /translation="MAVIDKYFTVVRDRGGSDLHLSISNPPMIRKDGEMMIIEGEAVL
                     THESVDKLLREIMPDRN"
     gene            325..1203
                     /locus_tag="VJC18_03505"
     CDS             325..1203
                     /locus_tag="VJC18_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44476.1"
                     /translation="MKYYLRWFIICSLLLVGMHCSSTTTPADNDDSDDDDDGDNTPIV
                     TASNIVADHSAVATFDTGIPTQYVTDAKNNLHIYYGHTSHGSQPVSGLEMLQSPYNTG
                     LDLTENESADLGHNGDTSWVTITEEAIANDSTINVVIWAWCGGVADNTESGINTYLTA
                     MNQLETTYPNITFVYMTGRHWNEDGSASVDGEGAGTNTHIEEMNDLIRAYVDAHDKVL
                     YDFADIEEYDPAGTQYSETSDYCSWCANWCTENASPVCESAVECAHAHPYNCYIKGKA
                     FWYLLARIAGWDGEAL"
     gene            complement(1336..3258)
                     /gene="dnaK"
                     /locus_tag="VJC18_03510"
     CDS             complement(1336..3258)
                     /gene="dnaK"
                     /locus_tag="VJC18_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HLD44477.1"
                     /translation="MSKKSKIIGIDLGTTNSCVSIMEGSEPKVIANQEGTRTTPSVVA
                     FTKDGEVLVGQSAKRQAITNPERTIYSIKRFMGRRKDEVPEEIKLVPYKVIDHKSGGA
                     AIDIDGKIYSPEEISAKILQKLKQAAESYLGETVTEAVITVPAYFNDAQRQATKDAGR
                     IAGLEVRRIINEPTAAALSYGLDKKKNELIAVYDFGGGTFDISVLEVGDNVVEVVATN
                     GDTHLGGDNIDQLIIDFLIAEFKRDQGFDVSKDKMVLQRLKEAAEKAKIELSSAMETD
                     INLPFLTADASGPKHMNLKMTRSKLEQICENIIARSFEPCRKCLSDAGKKASDINEVI
                     LVGGQTRMPRIRDEVKKFFGKEPHMGVNPDEVVADGAAVQGGVLSGDVKDMLLLDVTP
                     LSLGIETLGSVFTKIIDRNTTIPVKKSQIFSTAADSQTSVEVHVLQGEREMAASNRSL
                     GRFHLDGIPPSPRGIPQIEVTFDIDANGIVNVTAVDKATNKEQRITITASSSLSKDDI
                     DKMVKEAKENEGEDKKKRESVENKNKLESLTYEVEKQLKEYRDKLSADDVKKIEAELE
                     NGKKVLESGDSDKMKKAFDDLQQAALKIGEVLYKQQQAQQAGAQQGQSGPNPDAKSEP
                     KKEDDVVDAEFEESKD"
     gene            3472..4131
                     /locus_tag="VJC18_03515"
     CDS             3472..4131
                     /locus_tag="VJC18_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44478.1"
                     /translation="MVDRLEIKYSNQTYRVDASRDALEEERKESQDEEGDGQPNDDKF
                     GSLRDGQDLQRLFEKNQLWNRNIQIKVEDIDKIKVLGLNLKTEPSLLKIRIFLYDGAV
                     IPTAIVSVSRSLAFQIRGLKNAAFVDVNMLTKGTVLSVTVPKDENQIDEEITKITGVP
                     REKTLSRTFKMLISKRNWLQKFGLQDPVSKKINNEIIWVYITTIALMTLLSFGVLYLL
                     T"
     gene            4142..>5164
                     /locus_tag="VJC18_03520"
     CDS             4142..>5164
                     /locus_tag="VJC18_03520"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLD44479.1"
                     /translation="MAKFEPKPFGKYFLTDRIAIGGMAEIYKAKTFGVDGFEKILAIK
                     KILTHYSSDKEFITMLSDEAKLVVNLSHANIVQVYDLGRVGNDYFISMEFIDGINLRD
                     LIEKSRDLDEPIPIDIAIYIVSEVCRGLDYAHNKKDTSGSPLNIIHRDISPQNILVSY
                     DGGVKIVDFGIAKAALNLSQTHAGILKGKVNYMAPEQAFGKPMDGRSDLFSLGIVLYE
                     LLTNKRLYFGETQMEILKQIRNKKINEASFEDDVPDFLKPVLAKVLAYATNKRHETAA
                     DLQIELTRILYATYNDFTPRRLIDLLHRWFADRKEHAGASHEPDEGDTVLVSATQHDV
                     PQVNLVH"
BASE COUNT         1300 a         1269 c         1199 g         1396 t
ORIGIN      
        1 cattgcggtc tggcatgatt tcgcggagca gtttgtcgac gctttcgtga gtcaggactg
       61 cctctccctc aatgatcatc atttcgccat ctttgcgaat catgggcggg ttcgaaatac
      121 tcaaatgaag atcgcttcca ccgcgatcgc gaacaacagt aaaatattta tcaataactg
      181 ccataaaagc tccttgttag accttaattt taaatcatta ccatgaattt gcaatgaaaa
      241 agtgggggga cattccttga tcattattcg acatttagat aagtatcaaa agcggaatcg
      301 cataaacctg actaaggggg ccaaatgaaa tattatttac gttggtttat tatatgttcg
      361 ctcttgctgg tggggatgca ttgttccagc acaacaactc cagctgacaa cgatgactcg
      421 gatgatgatg acgatggtga taacactcct attgtgacgg caagcaacat tgtggccgat
      481 cacagtgccg tagcaacctt tgataccggt atccccacgc aatacgttac tgacgccaag
      541 aacaacctgc acatttatta tgggcatacc tcgcacggca gtcaaccggt ttcgggactg
      601 gaaatgttgc agtctcccta taacaccggt ctggatctga ccgaaaacga aagcgctgat
      661 ctcggacaca atggggacac cagttgggtc acgattacgg aggaggccat tgccaatgac
      721 agcacgatca acgtcgtgat ctgggcctgg tgcggtgggg tggccgataa tacggaatca
      781 ggcatcaaca cttatctgac tgccatgaac cagttggaaa caacttatcc caacatcact
      841 tttgtctata tgacagggcg ccattggaac gaggatggga gcgcctctgt ggatggcgag
      901 ggcgctggca cgaacacaca tattgaggaa atgaacgatc tcatccgcgc ctatgttgac
      961 gcgcacgaca aggttcttta tgactttgcc gatatcgagg aatacgaccc cgcgggcacc
     1021 cagtacagcg aaacatccga ttattgttcc tggtgcgcaa actggtgtac agaaaacgcc
     1081 tcaccggtct gcgaaagtgc tgtggaatgc gcgcacgcac acccatacaa ctgctacatc
     1141 aaggggaagg ccttctggta tttacttgcc cgcatagcgg ggtgggacgg cgaagcgcta
     1201 taatcaaccc atctactgcg tttcccggtc tgtctgcaat cctcacgtat tggcatatac
     1261 gctccggtta cagacagacc gggcgccttg tatctgggct gtttctagcg cttcgaccat
     1321 ttcattatct cttctctagt cctttgattc ctcaaattcg gcgtccacaa cgtcgtcttc
     1381 ttttttgggc tctgacttgg catcagggtt ggggccgctt tgtccttgtt gcgccccggc
     1441 ttgttgtgcc tgctgttgtt tgtacagaac ctcaccgatc ttgagcgcgg cttgttgcag
     1501 gtcgtcaaac gcttttttca ttttgtcgga atcgccagac tcaagcactt tcttgccatt
     1561 ttcgagctcg gcctcgattt ttttaacatc gtcggcagag agtttgtctc tatactcctt
     1621 gagctgtttt tcgacctcat aggtcaacga ctcgagtttg tttttgttct cgacagattc
     1681 acgtttcttt ttatcttcgc cttcgttctc tttggcctcc ttgaccattt tatcgatatc
     1741 gtccttgctg agactggaac tggccgtgat ggtgatgcgt tgttctttat tggttgcctt
     1801 atcgactgct gtgacattga caatgccgtt ggcgtcgata tcgaacgtga cttcgatttg
     1861 cgggatgcca cgcggagaag gggggatgcc atccagatga aagcgtccca aactgcgatt
     1921 ggaagcggcc atctcacgtt caccttgcag cacatggacc tcgacactgg tttggctatc
     1981 agccgctgtg gaaaatatct gcgatttttt gactgggatc gtggtgttgc gatcgatgat
     2041 tttagtaaac acactgccca gggtctcgat gcccaacgaa aggggggtga cgtcgaggag
     2101 taacatgtct ttgacatcgc cagacagcac accgccttgc actgcagcgc cgtcggccac
     2161 gacttcgtcg gggttcacac ccatgtgagg ttctttgcca aaaaactttt taacttcgtc
     2221 tcgaattctg ggcatgcggg tttgcccacc aaccagaatg acctcgttga tgtcgctggc
     2281 ctttttgccg gcatcgctca aacattttcg gcaaggttca aaggaacggg cgatgatgtt
     2341 ctcgcaaatt tgctcgagct tggaacgggt cattttgagg ttcatgtgtt ttggcccgct
     2401 ggcatcggcc gtgagaaagg gcaggttgat atcggtctcc atggcgctgg agagctctat
     2461 cttggccttt tcggcagcct ctttgaggcg ttgcaaaacc attttatcct tcgaaacatc
     2521 aaagccctga tctcttttaa attcggctat cagaaaatca atgatgagct ggtcaatatt
     2581 atcaccaccc agatgcgtgt cgccattggt ggccacgacc tcgaccacgt tatcacctac
     2641 ttcaagaacg gagatgtcga aggtgccacc accaaaatcg tatacggcaa tgagctcatt
     2701 cttttttttg tcgaggccgt aactcaacgc ggcggccgtt ggttcgttga tgatacgccg
     2761 cacctcaaga ccggcaatcc tccccgcatc ttttgtggcc tgacgttgcg catcgttaaa
     2821 gtacgcagga accgtaatga cagcttcggt gacagtctcg ccaagatagc tctcggcggc
     2881 ctgtttgagc ttttggagaa ttttggcaga aatttcttcg ggggagtaga tcttgccatc
     2941 gatgtcgata gcagcaccgc cactcttatg gtcaatcacc ttatagggta ccagtttgat
     3001 ttcctcgggc acctcgtcct tgcgacgacc cataaagcgc ttgatagagt agatcgtgcg
     3061 ctctggattc gtaatggctt gacgtttggc cgactgtcct accaatacct caccatcctt
     3121 tgtaaaagcc acgacagacg gtgttgtacg cgttccctcc tggttggcaa tcaccttggg
     3181 ttctgacccc tccatgattg agacacacga attcgttgtt cccaagtcga taccaatgat
     3241 tttagatttt tttgacattt aaaaactccc attttttcaa ttagttacaa aatcaactgt
     3301 tcacctcatg catgcattca agttaagtcc agaatatcct ttgtcaatcg tttaacatat
     3361 attttttgtt gtggtgtcca caccccaaat gtgatacagt tgaacacaac attatgcatt
     3421 gtttcattga ttcattgttt cattgattca ttgttttatt gccctttttc tatggtcgat
     3481 agactcgaaa taaaatatag caatcaaaca tatcgtgttg atgcttcacg ggatgccctt
     3541 gaggaagaac gtaaagagtc acaggatgaa gagggcgacg gtcaacccaa cgacgacaaa
     3601 tttggctctt tgagagacgg gcaagatctg caacggctat tcgaaaaaaa ccaactgtgg
     3661 aatcgcaaca ttcaaatcaa ggttgaggac atcgataaaa tcaaggtgct gggattgaat
     3721 ttaaaaactg agccgtcctt attgaaaatt cggatttttt tgtacgatgg tgccgtgatc
     3781 cccacagcaa tcgtttcggt gtcacgcagt ctggcctttc aaataagggg gctcaaaaat
     3841 gcggcttttg tagatgtcaa catgttgacc aagggaacag tcctgtctgt taccgtgccc
     3901 aaggatgaaa atcaaatcga tgaggagata accaagatca cgggtgtgcc cagggaaaaa
     3961 acgctgtctc gcacattcaa aatgttgatc tccaaaagga attggttaca gaaatttgga
     4021 ttgcaagacc ccgtatcaaa aaaaattaat aacgagatca tttgggtcta catcacgacc
     4081 attgccctga tgacactgtt gagctttggt gttttgtatt tgctgaccta aaaagagaac
     4141 tatggccaag tttgaaccaa aaccattcgg aaaatatttc ctcactgacc gcatagcgat
     4201 cggtggcatg gccgaaattt ataaggccaa aacatttggc gttgacggct tcgaaaagat
     4261 tctcgcgatc aaaaaaatcc tgacgcatta ttcctccgat aaagaattca tcacgatgct
     4321 ttccgatgaa gccaagctcg tggtgaatct gtcacacgca aacattgttc aagtctacga
     4381 tctggggcgc gtgggcaacg attatttcat ctccatggaa ttcattgacg gcatcaattt
     4441 gcgtgatctc atcgaaaaaa gtcgtgacct cgatgagcct attcccatcg acatcgccat
     4501 ctatatcgtt tcggaagtgt gtcgtggact cgattacgcc cacaacaaaa aagacaccag
     4561 cggatcaccc ctcaacatca ttcatcgtga catctcacct caaaatatcc tcgtgtctta
     4621 cgatggtggc gtgaaaatcg tggatttcgg catcgccaag gccgcactca acctgtccca
     4681 aacacacgct ggcattctca agggaaaggt caactacatg gccccggaac aggctttcgg
     4741 caaacccatg gatggcagat cggatttgtt ttcgttgggc attgtcttgt acgaactgct
     4801 caccaacaag cgtttgtact ttggcgaaac ccagatggaa attttaaaac aaattcgcaa
     4861 caagaagatc aacgaagctt cttttgaaga tgatgtgccg gattttctca aacctgtcct
     4921 ggcaaaagtt ttggcctatg ccaccaacaa acgtcacgaa acagcggcgg acctgcagat
     4981 agaactcaca cgcattttgt acgccaccta caacgatttc accccgagaa gactcatcga
     5041 tcttctccat cgctggtttg cagatcgcaa agaacatgcc ggtgcatcac acgaacccga
     5101 tgaaggcgat actgtattgg tcagcgccac acaacatgat gtgccgcaag tcaatctcgt
     5161 tcat
//
LOCUS       DATDUN010000263         9554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_303293, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000263 DATDUN010000000
VERSION     DATDUN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303293"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1869
                     /locus_tag="VJC18_03525"
     CDS             <1..1869
                     /locus_tag="VJC18_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44480.1"
                     /translation="CQQVSPLVLQFRDLLQNTLHEHQTQFSEVIYEDSSFVGPGCESE
                     KTEGEGTSVGSFYVTPLRVDNELLGYFALGHVKKNAFPRFKKNLLDDFCIHLSHGIRN
                     LLNKSLIIEQMALLEQEKHKTETEKRKLDIILAGMKEGLILSDARQNVLVVNRAACDM
                     LGLENAEGQSRAREFIVRAVLDKGTLAKPRDRDVLLKRPERQVLHISASPIVDEKGIF
                     VAQATLLTDVTREREVERMKSEFVSVVSHELRTPLTAIREAIALIIDGIAGTVNDKQR
                     HCLDVALRDVDRLGRMINDLLDLSRIESGKIGLKRISCSAEYLITCVHTILAAKAQSD
                     NIQLVVAVSENVPPVFADPDTIIQVLINLVGNAFKFTPEGGTVRVSCGTSREAHPVSR
                     ISPDVFSRDTNNEIRMTDRNFVEFSVTDTGPGIPESDQKKLFQKFSQLDMSVTHRVGG
                     TGLGLAISKELVEKHGGTIRVVSDPGKGSCFSFTIPVFTQELFTIDLIQKELDRVRDN
                     RGCLAVILMNFAFPQELSSQNREQVLAHERALRSLMRKKEDVVCVYLERSLLCIVEAN
                     QDGANSLVLRIRNEMIDINSVQIAVYPADGQRAEDLFNRVTLRVPRDTGSVMYNQV"
     gene            1866..2255
                     /locus_tag="VJC18_03530"
     CDS             1866..2255
                     /locus_tag="VJC18_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD44481.1"
                     /translation="MTKKILIIDDEDHIVEMLALRLESLGYKVLASHDGMSGLEMARA
                     ELPDLILLDVMMPKMDGYTVCKLLKNDEAYKHIPIIMLSARSQGVDQTIGADCGADDY
                     ATKPFKHQELFEKINKHLKEMGEGGVG"
     gene            2259..3167
                     /locus_tag="VJC18_03535"
     CDS             2259..3167
                     /locus_tag="VJC18_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HLD44482.1"
                     /translation="MRDSDNKNLITLLTKIYQARGLDFTQYKISMVQRRVRSRMLKCN
                     VSTYEDYFRILDQQPDELGHLIQSFSIQVTELFRNPESFNAIRDIVIPQIIISKREKQ
                     HKIIKAWSCGCSTGDEPYSLAMLFLEKLGAARDRFILTILGTDIDDRAIREAGDTEFE
                     KDQISALNQDIIRNYFVKHAHSKFKIADPVRNLVRLRHHDVINDIPYMHCDVILCRNV
                     LIYFNKELQQETQLKFYDCLNPGGFLVLGMTESLIGSASEYFEPVNNSLRIYRRPIAR
                     TTGAEKDREVLSQNDIDRIIKEMTEE"
     gene            3276..5300
                     /locus_tag="VJC18_03540"
     CDS             3276..5300
                     /locus_tag="VJC18_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HLD44483.1"
                     /translation="MTPQSANKTIVTLNPEPSLVHSITLAQNNLINQGHKDGYWWYTL
                     EANDSINAEYIMLLKYLEIDDKQTEKAICRWILHNQNHDGSWSLYYGAPGDLSATVEC
                     YIALKMAGFDSNQPRMAQARKFIMQLGGVSKIRVFSRIHLALFGLVDWKICPTMPVAL
                     IQFPEWAPVNIYEFSSWARASIVPLLVIMDQKKTKKITGLSIDELYVDSQKKEKWIYP
                     NDAGVLSIENLFIKIDKALHVADTLKFKPLRKQSLKKCEDFIRSHLGVTEDIYPAMFY
                     GVLALNSLGYPMNDTNIEKALMGLKSFQVIMSHKELHAVPFQDSSTIDYNLFKKQADD
                     AHRDYMVYQQCCISPVWDTAWAATTLLKSGFPADSPILLKTARWLISKQITDVVGDWG
                     IKNPGSEPGGWSFEFINKYYPDVDDTIEVLTFLHDVGLPYHVLKRPIEKGVNWLLSMQ
                     CSNGGFAAFDKDNDLVLLNKIPFSDHGACLDPPTVDITGRMIEFLIKVLDFDGSHEAV
                     LLAADFITERQEKDGSFWARWGVNYIYGTWCALEGLCCLSRKKDELIISRAVHWLKSI
                     QNTDGGFCESSASYNLGRFLPLPESVPSQTAWALMGLVAAGQQFSPEARKAADFLISA
                     QKEQGGWDEKYYTGTGFPGHFYIRYHGYRYYFPLLALSKYRDAIVKRIRK"
     gene            5334..5564
                     /locus_tag="VJC18_03545"
     CDS             5334..5564
                     /locus_tag="VJC18_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44484.1"
                     /translation="MTEKQGFRITGRFLKMFFLSLFFALAMGAFFFFLDQAGIFPRAN
                     PDKPGYFLIGLGTILGYNLGIIVSSRIFLKRK"
     gene            complement(5673..6794)
                     /locus_tag="VJC18_03550"
     CDS             complement(5673..6794)
                     /locus_tag="VJC18_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44485.1"
                     /translation="MDKHTIIKSVFLGMFACVITACGDMGLDVNGNSGSQTYTETNEE
                     AMVTAAVESLTSSISSSESASASSYASLTKGPAYLTVGNPINTSYATLAKRFRCEELS
                     SVNEVFSCDNATGAMTREVSFEDCDLIDSNKPGTLSGGFTNTVENGGSGLCDGLDHID
                     FAKMVMGRDGLNAVHTHTIDDGGMVFTLDVSDTVTITMSGNDVVTFTDPVDEDVDGVA
                     ESVTATVVKDINRVTVENGVEVHNVTVSTREESFTPTDEEGTLLEIVNLNYPVHNLVF
                     DETDKVETRTVTSGELVIDRSHSDLRIVLGVGAEGLTFYPQDHCGPFEGTATITGYVL
                     NEEGNYVEAGSGQIVYADGEVQTAEYNGIELNLKPRPCS"
     gene            7046..7741
                     /locus_tag="VJC18_03555"
     CDS             7046..7741
                     /locus_tag="VJC18_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HLD44486.1"
                     /translation="MQITKHYLFLFFVGAFFISGFFPLITFAQTQIDGSPTQDNVLQT
                     RPAPFGNEQRRMRPDHERRRAYDQNGDSRERRMRPNKANRPNLVQELQLTQEQKQKMQ
                     GLRQSHMQALQQRKDSIKQARDAFEQAIKGTKSDEELRSLYQKFEEAKTSMAKEQFEQ
                     ILTIRSLLTDEQKSKFVGFGQQMLGDNPAGARGPGQNRGFRDRDSLQNQPSGPPRGAD
                     PQNHGGTNIINEN"
     gene            7750..8280
                     /locus_tag="VJC18_03560"
     CDS             7750..8280
                     /locus_tag="VJC18_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLD44487.1"
                     /translation="MQKSQINQLIQKYTQSEIKDFSELYNVYHKMIRSILYKMCAPED
                     MEDLMQDVFLKIINGLAQFNQKSSLKTWIYRVACNHALDHLRKKKPITVDYDLQKIGG
                     QNANSGGSNQDIVIKTLQKLSPDHRAVLVLVCLEELTIEEASMAIGVPQGTIKSRLHH
                     AKGKMYELLSQQGVNL"
     gene            8277..8717
                     /locus_tag="VJC18_03565"
     CDS             8277..8717
                     /locus_tag="VJC18_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44488.1"
                     /translation="MIKQPVFDSDKEKSLDVFLKNNNPAPPSPSHGEGERILQNVINA
                     TPSSLGNKAACRKTTPFASILFLRWLVPVAATAAIGFLVINHNFSSIDNKVTSVSDDN
                     LSAYLEETLTGMYEDSYFDQGEDNVASLSTQYYQIPDQINGEGY"
     gene            8796..9296
                     /locus_tag="VJC18_03570"
     CDS             8796..9296
                     /locus_tag="VJC18_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44489.1"
                     /translation="MTNKSFALLGFRLLSLYFIVTGVIAFPIALSSPLMVDGSFFAIF
                     LPYLGFGLPALTAGLLLWFVAPYLVKYVTPKEEGPCSFSGMNVVDFYQVAFVIVGLFL
                     LAYALPECLLVIFSLFDLPCKVSDMTICRCPIGQLVLPLLKILMGLWFLFRSHYLARS
                     LLKIHK"
BASE COUNT         2615 a         2095 c         2219 g         2625 t
ORIGIN      
        1 tgtcagcaag tctctcccct ggttttacag ttcagggatc ttttgcagaa caccctacat
       61 gagcatcaaa cacagttctc cgaagtgatt tatgaagact cttcatttgt ggggcctggt
      121 tgtgagagcg agaaaacaga aggagagggg acttcagtgg ggtctttcta cgtgacgcca
      181 ctcagggttg ataatgaatt gctgggttat tttgccttgg gacatgtcaa aaagaacgcc
      241 tttccccggt tcaaaaaaaa tcttctggat gatttttgta ttcacctgtc ccatggcatc
      301 cgcaatctcc tgaacaaaag tctcattatc gaacaaatgg ctcttttgga gcaggaaaag
      361 cataaaacag agaccgaaaa acgaaagttg gatatcattc ttgctggcat gaaagaggga
      421 ctcatccttt ctgatgccag acaaaacgtt ctggttgtca atcgagcggc ctgcgatatg
      481 ctgggtcttg aaaacgccga gggacaatcc cgtgccagag aattcattgt ccgggcagtc
      541 cttgacaagg gaaccttggc gaagcctcga gacagggatg tcctcttaaa acgaccggaa
      601 cggcaagtcc ttcatatcag cgcgtcacct attgttgatg aaaagggaat ttttgtagcg
      661 caggcaactt tgttgactga tgtgacccga gaaagggaag ttgaaagaat gaaatcggaa
      721 tttgtcagtg tggtgagtca cgaactgcgt acccctctca cggcgattcg ggaggccatt
      781 gccctgatca tcgatgggat cgcagggacc gtcaacgata agcaacgaca ttgtcttgat
      841 gtggccttga gggacgtgga tcgcctgggg cgcatgatta acgatctcct cgacctgtca
      901 cgcattgagt ctggcaagat cggcctcaag aggatatcgt gctcagcaga atatttgata
      961 acgtgtgtgc acacaatcct tgccgccaag gcacaaagcg acaatatcca gttggtggtt
     1021 gctgtgtccg aaaatgttcc tcctgttttt gcggaccccg acacgatcat tcaagtgctc
     1081 atcaatctgg tgggaaatgc gttcaaattt acaccggaag ggggaactgt gagagtgtcc
     1141 tgcggcacat ctcgtgaagc gcatcccgta tctcgtatct caccagatgt tttctcacga
     1201 gatacgaata acgaaatacg aatgacggac agaaatttcg tggaattttc tgtcacagat
     1261 accggccccg gcattcccga atccgatcaa aagaaacttt ttcagaaatt cagtcaactc
     1321 gacatgagcg tgacgcaccg ggtgggcggg acgggtttgg gtctggccat cagcaaagag
     1381 cttgttgaaa aacatggggg aaccatacgt gtggtgtcgg atcccggcaa ggggtcttgc
     1441 ttttcgttta ccatccccgt attcacacag gaactcttca ctattgatct gatccagaag
     1501 gagttggatc gtgtgagaga caatcgaggg tgcctggcgg ttatccttat gaactttgct
     1561 tttcctcaag aattatcctc gcagaacagg gaacaggtac tggcgcatga aagagcgttg
     1621 cgatcactca tgcgcaaaaa ggaagacgtg gtgtgtgttt atcttgaaag gagtttgctt
     1681 tgcattgttg aagcgaatca ggatggggca aatagtctgg ttctcaggat cagaaatgaa
     1741 atgattgata tcaattctgt tcaaattgcc gtttatcctg ccgacggcca acgtgcagag
     1801 gatttgttta acagggtcac acttcgtgta cctcgagata cgggaagtgt gatgtacaat
     1861 caggtatgac aaaaaaaata ctcattattg atgatgagga ccacatcgta gagatgcttg
     1921 ccctgcgact tgagtcgctg gggtataagg tgttggcatc acacgatggt atgtctggac
     1981 tcgaaatggc acgagcagaa cttcccgatc ttatcctgct tgacgtgatg atgccaaaaa
     2041 tggatggcta tacggtgtgc aagctcctca agaatgacga agcgtacaag cacatcccga
     2101 tcatcatgtt gtcagccagg tctcaaggtg ttgaccagac aattggtgcc gactgtggcg
     2161 ccgatgatta cgccacgaag ccgtttaaac atcaggagtt attcgagaaa ataaacaaac
     2221 acctcaaaga gatgggtgag ggtggggtcg gttgaaacat gagagattca gacaataaaa
     2281 acctgatcac actccttaca aaaatatatc aggcccgggg cttggacttt acgcagtata
     2341 aaatcagcat ggtgcaaagg cgtgtccggt cgcggatgct caagtgcaat gtgtctactt
     2401 acgaagacta ttttcggatt ctcgatcagc aaccagacga attgggtcat ttgatccaat
     2461 cgttttccat tcaggtaaca gaattattca gaaatccaga gagttttaac gccattcgcg
     2521 atattgtcat tcctcaaatc attatttcga aaagggagaa acagcacaaa atcatcaaag
     2581 catggagttg tggttgttca acaggagatg agccttattc gttggccatg ctttttttag
     2641 aaaaattggg ggcggcgagg gacaggttca tactcacaat tttgggaacc gatatagacg
     2701 acagggccat cagagaagca ggggacacag agttcgaaaa ggatcaaatc agtgccctta
     2761 accaggatat catccgcaat tattttgtaa aacatgctca cagcaaattc aaaattgcag
     2821 accctgttcg gaatctggtc agactgaggc atcacgacgt gatcaatgac ataccctata
     2881 tgcattgtga tgtgatcctg tgtcgtaacg tgctcattta ttttaacaaa gaattgcaac
     2941 aggagacaca attgaaattc tatgattgcc tcaatccggg aggttttttg gttttgggaa
     3001 tgacagaatc tctcatagga agcgcgtctg aatattttga acccgtgaac aattcactac
     3061 gtatttaccg acgccccatt gccaggacaa caggggcaga aaaagaccgc gaggtcttat
     3121 cacaaaacga tatcgatcgt attatcaagg aaatgacaga ggagtgaggg cgtgttagtc
     3181 atatccacaa tgtcgcagaa tatatattat gttaaataag tagcttttca actcattgac
     3241 ttcttggttc aattaaaata aatctcgcgt ctctcatgac tcctcaatct gcaaataaaa
     3301 ccatcgtcac tctaaatcca gaaccctctc ttgtgcattc catcacactg gcacagaaca
     3361 atttgataaa ccaaggccat aaagatggtt actggtggta cacccttgag gccaacgatt
     3421 caattaatgc tgaatacatc atgttgctca agtatctgga aattgatgat aaacagacag
     3481 aaaaagccat ttgtaggtgg atattgcata atcagaacca tgacggttca tggagtcttt
     3541 actatggagc ccccggagat ttaagtgcga ccgttgaatg ctatattgca ctaaaaatgg
     3601 ctggttttga ttcaaatcag ccaaggatgg ctcaagcccg caaatttatt atgcaattag
     3661 ggggtgtctc aaaaattcgt gtgttttcac gcattcatct ggcccttttt ggtctggtgg
     3721 actggaaaat atgccccaca atgcctgtcg cgctcattca atttcctgaa tgggcccctg
     3781 tcaatattta cgaattttcc agttgggcgc gggcaagcat tgttcctcta ttggtcatca
     3841 tggaccaaaa aaagacaaaa aagattactg gtctttccat tgacgagctg tatgttgatt
     3901 ctcaaaaaaa agaaaaatgg atatatccca acgacgccgg tgtgctcagt attgaaaatc
     3961 tttttattaa aatagataag gctctgcatg tagctgacac gctcaaattc aaaccactta
     4021 gaaagcagtc attaaaaaaa tgtgaggatt tcatccgttc gcatttgggc gtcacagaag
     4081 atatctatcc agccatgttt tacggtgttc tggcgcttaa cagtcttggt tatcccatga
     4141 acgataccaa tatcgaaaaa gcgcttatgg gattaaagtc cttccaagtc ataatgtcgc
     4201 ataaagagtt gcatgctgtg ccgtttcagg acagctcgac gattgactat aacttattca
     4261 aaaaacaggc tgatgatgcc cacagagatt atatggtcta tcagcaatgt tgcatctccc
     4321 ccgtatggga tacggcgtgg gcggctacaa cacttttaaa atcgggcttt cccgccgata
     4381 gccccatact gttaaaaacg gcccgctggc ttatcagcaa gcagatcacc gatgtggttg
     4441 gcgattgggg tattaaaaat ccagggtccg aaccaggtgg ctggtcattt gaatttatca
     4501 ataaatatta tcccgatgtt gacgatacca tcgaggtcct cacttttctg cacgatgtag
     4561 ggctccccta ccatgtcttg aaaaggccga ttgagaaagg cgtcaattgg ttgctctcca
     4621 tgcaatgctc caatggtggc tttgcggcat ttgacaaaga caacgatctg gtgttgctca
     4681 ataaaattcc tttttctgac catggtgctt gtctcgatcc tcccactgtc gacatcacgg
     4741 gccggatgat agaatttttg atcaaggtct tggacttcga cggcagtcac gaggcggttt
     4801 tgctggcggc agacttcatc acggagcgtc aggaaaaaga tggttccttt tgggcccgct
     4861 ggggtgtcaa ttatatctac ggtacctggt gtgccctgga aggcctgtgt tgtttgagcc
     4921 gcaaaaaaga tgaactgatc atcagtcgcg ccgtccattg gctcaaaagc attcaaaaca
     4981 ccgatggtgg cttttgtgaa tccagtgcgt catacaattt gggacgtttt ttaccactgc
     5041 ctgaatcggt gccatctcaa acagcttggg ccctgatggg acttgttgcg gcgggtcaac
     5101 agttttcacc tgaggccaga aaggctgcag attttctgat ctctgcgcaa aaagaacaag
     5161 gtggttggga cgaaaaatac tacacgggca cggggttccc cggccatttt tacattcgct
     5221 accatggtta tcggtattat tttccattgt tggcgcttag caaatatcgc gacgccattg
     5281 taaaacgtat tcgaaaataa aggacactct gaaggtgtcc tgaagggggg actatgacgg
     5341 agaaacaggg ttttagaatc accggccgtt tcttgaaaat gttttttttg agcctctttt
     5401 ttgccctcgc gatgggggcc tttttcttct ttctggatca ggcaggtatt ttcccgaggg
     5461 ccaatcccga caaacccgga tatttcctga tcgggctggg tacgattctg ggttataatc
     5521 tggggatcat cgtcagcagc cgtatttttc taaagcgtaa atgatttatc gaacaaggaa
     5581 catgaagatg gggctaatca ccctatttca tgattccttg ttgcagcctc agccaaagga
     5641 ctgatcccgc tctgcgggac gatattcgat attcaactac agggccttgg tttcagattg
     5701 agttctattc cattgtattc tgcagtttga acctctccat cggcatacac gatttgcccg
     5761 gaaccggctt caacgtaatt gccctcctcg ttcaggacat acccagtaat ggtggcggtg
     5821 ccttcgaatg gaccacaatg atcctgcggg taaaaggtca acccttctgc acccactccc
     5881 agaacaattc tcagatcgga atgactgcgg tcgatcacca gttcaccgga ggtaaccgtg
     5941 cgtgtttcaa ccttgtctgt ttcatcaaac acgagattgt gaactgggta attcagattg
     6001 actatttcca gcagagttcc ttcttcgtcg gtgggggtga aactttcttc cctggtcgat
     6061 acggtcacat tgtgtacctc cacgccgttt tcaacagtca cgcgattgat gtctttgact
     6121 acggttgccg tgacagattc agcgacaccg tccacgtctt catcaacagg gtcagtaaac
     6181 gtcacaacgt cgtttccact catcgtgatg gtgactgtat cggacacatc cagcgtgaat
     6241 accattcctc cgtcatcaat cgtatgcgtg tgtacggcat tcagcccgtc acgtcccata
     6301 accatcttgg caaaatcaat gtgatcaagg ccatcgcata aaccggagcc tccattctct
     6361 actgtgtttg taaagccacc agagagcgtt cctggtttat tactgtcaat caaatcacaa
     6421 tcttcaaaag agacctctct ggtcatcgca cccgtggcgt tatcacatga gaacacctcg
     6481 ttcacgctgg ataattcctc acagcggaaa cgtttggcca aagtggcata tgaggtgttg
     6541 ataggattcc caacagtcag ataggcaggc cccttggtca gtgatgcata ggaactcgca
     6601 gaggctgatt cactcgatga gatactgctt gtcaacgact ccacagcggc agtgaccatg
     6661 gcctcttcgt tggtctctgt atatgtttgc gacccgctgt tgccattcac atccaaaccc
     6721 atgtcaccac atgctgttat gacgcaggcg aacatgccca aaaacaccga tttgatgatt
     6781 gtatgcttat ccataaagat cctttcgtta tgactcaaaa gcgggatacc atagcccgct
     6841 catgagcgaa ttattgtttc attcaagtta gacgccagat gaagagaaaa gttcattctt
     6901 ttttcgcaag ttgaattgtg ttagtcatat caaatagatg attagacagc tcattttctg
     6961 tctttggaac tggaatcaat attatgttat cagaagtgaa ccttttgctt tattgaatcg
     7021 tctaaaaatg taaaaggaca attctatgca aatcaccaaa cattatttgt ttctcttttt
     7081 tgtgggggcc tttttcatct caggattctt tccccttatc acgttcgcac aaacacaaat
     7141 tgatgggtca ccaacacaag acaatgtact gcaaacgcgc cctgccccgt ttggcaatga
     7201 acaacgcaga atgcgacccg atcacgaaag gcgtcgtgct tacgaccaaa atggggatag
     7261 cagagaacgt cgcatgcgtc ccaacaaagc aaaccggcca aatttggttc aggagttgca
     7321 attgacccag gagcaaaaac aaaaaatgca agggctgagg cagtctcaca tgcaggcact
     7381 tcagcagaga aaagacagca ttaagcaggc ccgtgatgcc tttgaacagg ctattaaagg
     7441 cactaaatcc gatgaagaac tcaggtcatt gtatcaaaaa ttcgaagagg ccaagacatc
     7501 catggccaag gaacagtttg agcagatcct cacgatcaga tcactattga ccgatgaaca
     7561 gaagtctaaa tttgttggtt ttggacaaca gatgctcgga gacaatccgg cgggtgcgcg
     7621 tggtcctggg caaaatcgag gatttcgcga tcgcgattct cttcagaatc aaccaagcgg
     7681 gcctccacgg ggagcagacc cgcagaatca tggagggaca aacattatca acgaaaattg
     7741 aatgaaaaaa tgcaaaaatc tcaaatcaac cagctcattc agaaatatac tcaatcagaa
     7801 ataaaggatt tttctgagtt gtataatgtc tatcacaaaa tgatccgctc tatattgtac
     7861 aaaatgtgcg ctccggaaga catggaagat ctgatgcagg atgttttttt gaaaatcatc
     7921 aacggattgg ctcagtttaa tcagaaaagc tcattaaaga cgtggatcta tcgggttgct
     7981 tgcaatcacg cgcttgatca tttgcgaaag aaaaagccca tcactgttga ttacgacttg
     8041 caaaaaattg ggggccaaaa cgctaattct ggaggatcca atcaagatat tgtgatcaag
     8101 accctgcaaa agctcagtcc tgaccacagg gcagtactgg ttttggtttg tttggaagaa
     8161 ttaaccattg aggaggcttc tatggcgatt ggtgtacctc aaggtacgat caagtcgcga
     8221 ttgcatcatg caaagggaaa aatgtatgag ctattgtctc aacaaggggt gaatttatga
     8281 taaaacaacc tgtttttgac tcagacaaag agaaatcgtt agatgttttt ttaaaaaaca
     8341 acaatcctgc tccgcccagc ccttctcacg gggagggaga acgtatttta cagaacgtca
     8401 tcaacgctac gccctcaagt ttaggcaata aggcagcttg tcgcaagacg acaccatttg
     8461 cttccatcct ttttctcaga tggctggttc ctgtcgccgc tacggccgcc attggttttt
     8521 tggtgatcaa tcataatttt tcatcgatcg ataataaagt cacttctgta tctgatgata
     8581 atctgtctgc atatttagag gaaaccctga cgggaatgta tgaggactct tattttgatc
     8641 aaggtgaaga taatgttgct tcgctttcga cgcagtatta tcagatcccc gatcaaatca
     8701 atggagaagg ttattgatat ttattgttta atcacataga tgtaacgaac atcaggcaac
     8761 tctgacgaaa atcaaataat tcaggggggt tgattatgac gaacaaatcg tttgccttgc
     8821 ttggttttag gttgttatct ctgtatttta ttgtgacggg ggttattgct ttcccaatag
     8881 ctctttcaag cccattaatg gttgatggca gttttttcgc catcttttta ccctatttag
     8941 gatttggact cccagcactt accgcggggc ttcttttgtg gtttgttgcg ccctatttgg
     9001 tcaaatacgt tacccctaaa gaggagggtc cttgttcatt ttcggggatg aatgttgtgg
     9061 atttttatca agtcgccttt gtcattgtgg gtcttttttt actagcctat gccctccctg
     9121 agtgtttatt agttattttt tcattatttg acctgccctg caaagtctct gatatgacta
     9181 tttgcagatg tccaatcggt caactggtgc ttcctctgtt gaaaattctt atggggcttt
     9241 ggtttttgtt tcgcagtcat tatctggctc gttctttact gaagattcac aaatagggtc
     9301 gatcatttca accttgcaat ccctttcctt tcgtccctat caaggtatgt ggtacctgaa
     9361 tctgaatcaa caaatatgaa aacgaagttc tggaatacgt gtttaaaata tgtagatgcc
     9421 tatgatcttg tttacctgct ctgcgttgtt tcgagatgcg gctcggctcc aaacagggct
     9481 ggtgcgaaga tgttggtaac aggtgctaaa aattatggga caattggagg tggtcgatcc
     9541 gaatttctct tagt
//
LOCUS       DATDUN010000264        12425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_262990, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000264 DATDUN010000000
VERSION     DATDUN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262990"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="VJC18_03575"
     CDS             complement(<1..1795)
                     /locus_tag="VJC18_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLD44490.1"
                     /translation="MAKSVSDLYRLRKDFSRIKKIVEIPNLIEIQKRSYDTFLQFNVP
                     TDKRDAVGLHGVFSSVFPIRDFNNTASLEFVSYSFEKPRYDVLECRQRGMTFEAPMKV
                     VVRLVVWDNEIGSETPTIRDVKEQEVYFGTIPLMTENGTFIINGVERVIVSQLHRSPG
                     VFFEHDKGKTHASGKLLYSARVIPYRGSWLDFEFDTKDLLYVRIDRRRKIPATILLRA
                     MDMSVEEVLKYFYDSETIIFDGKKLKKKINEGLLRLQRATRDVKVPSTKEVLVKKDGK
                     FTARAIKRMNELKMTELELEDAEVLGCVVAHDVIDQSTGEVLVECNATVTATVLENLR
                     KANIASFDILYIDNLNVGGYIRNTLLVDKVNSPDEAVIEIYKRLRPGDPSTLEAAKAL
                     FANLFFTEGRYDLSKVGRLKINHKFGFDVPIECAVLRKEDILYITKYLCRLKDGFGTV
                     DDIDHLGNRRVRAVGELLENQYRIGLVRMERAIKERMSLQEIETLMPNDLINAKPVSA
                     VVKEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRDRAGFEVRDVHATHYGRI
                     CPIETPEGPNIGLIASLSTYARVNEYGFIETP"
     gene            complement(1990..2361)
                     /gene="rplL"
                     /locus_tag="VJC18_03580"
     CDS             complement(1990..2361)
                     /gene="rplL"
                     /locus_tag="VJC18_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HLD44491.1"
                     /translation="MTTQEIVEAVSKMTVLEVADLVKAFEEKFGVSAAAPMAVAVAGG
                     AAVAAAAEEKTEFNVSLKEIGANKINVIKEIRTITGLGLKEAKDLVEGAPSVVKESVD
                     KDEAQKIKEALEKAGATVEIK"
     gene            complement(2437..2961)
                     /gene="rplJ"
                     /locus_tag="VJC18_03585"
     CDS             complement(2437..2961)
                     /gene="rplJ"
                     /locus_tag="VJC18_03585"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HLD44492.1"
                     /translation="MNRTEKAAHIDEIRDCLAKSQLTILADYQGLPVAALTDLRRQLR
                     AKNSFFKVVKNRLAKIAVKDTAASVLGDSFVGNTAITISQSDPTGPAKVLEEFAKKNE
                     RLKIKVGLLNGKYLDSNGIKNLASLPSKEELIAKLLGSLNAPATNLVSVLCQIPRQLV
                     TVLNSVKGQKEQSV"
     gene            complement(3181..3882)
                     /gene="rplA"
                     /locus_tag="VJC18_03590"
     CDS             complement(3181..3882)
                     /gene="rplA"
                     /locus_tag="VJC18_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HLD44493.1"
                     /translation="MKIPKKQKEIRKKINSEKKYALADAMALVLQGATRKFDETVDVC
                     VRLGIDAKKSDQGIRGSVVLPHGLGKKVRVLALVKGEKETEAKEAGADFVGNTDMIEK
                     IQGGWLDFDSVVATPDMMGVVSKVGKILGPRGLMPNPKLGTVSFDVKKAIQDCKAGKV
                     EFRNEKAGNVHAPFGKISFGAEKLTDNLKVLLETIRKMKPATSKGVYLKNITLSSTMG
                     PGVAVDVNEVSKVAG"
     gene            complement(3890..4315)
                     /gene="rplK"
                     /locus_tag="VJC18_03595"
     CDS             complement(3890..4315)
                     /gene="rplK"
                     /locus_tag="VJC18_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HLD44494.1"
                     /translation="MARKPIAQIKLLCPAGQANPAPPVGPALGQHGVNIMEFCKKFNA
                     ATQKEVGMITPCVITVYSDRSFSFVLKTPPASILLMKAASVKKGAGDTGKSLVGKVTL
                     KQVEDIAKVKMPDLNCFTLDAAINTVKGTAKSMGIEVVA"
     gene            complement(4406..4936)
                     /gene="nusG"
                     /locus_tag="VJC18_03600"
     CDS             complement(4406..4936)
                     /gene="nusG"
                     /locus_tag="VJC18_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HLD44495.1"
                     /translation="MSKKWYVVHTYAGFEQKAKLSLEERVKSTQMQEHFEEILVPSES
                     VVEIRKGQKKTTTRKFFPSYILVKMDLTDETWHLVKDTPKITGFVGNSQSPPSIPEED
                     VLKITQQIDSGELKPKLKYNFEKGDSVRVTDGPFNTFNGIVEEVNPEKGRLKVLVSIF
                     GRATPVDLEFTQVEKN"
     gene            complement(4964..5335)
                     /gene="secE"
                     /locus_tag="VJC18_03605"
     CDS             complement(4964..5335)
                     /gene="secE"
                     /locus_tag="VJC18_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HLD44496.1"
                     /translation="MKNRKIVTICLILVAVFLYWVASQICELLFDWFNLAITRDYFVT
                     IPDLIGAVCAGVAFALTLRHQRFMSFLEEAVTELSKVTYPTTKESGQSAVVVVILVGI
                     ATTMLALYDLFWSTLTRWILN"
     gene            complement(5504..5576)
                     /locus_tag="VJC18_03610"
     tRNA            complement(5504..5576)
                     /locus_tag="VJC18_03610"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5541..5543),aa:Trp,seq:cca)
     gene            complement(5755..5910)
                     /gene="rpmG"
                     /locus_tag="VJC18_03615"
     CDS             complement(5755..5910)
                     /gene="rpmG"
                     /locus_tag="VJC18_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HLD44497.1"
                     /translation="MNLIVTVSLECEVCKKRNYRFKINKDKKDKKLKLKKFCKYCKAH
                     TMHVQVR"
     gene            complement(6061..7251)
                     /gene="tuf"
                     /locus_tag="VJC18_03620"
     CDS             complement(6061..7251)
                     /gene="tuf"
                     /locus_tag="VJC18_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HLD44498.1"
                     /translation="MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAITLVLAVKGRATF
                     VAYDQIDKAPEEKERGITINTAHVEYQTDTRHYAHVDCPGHADYIKNMITGAAQMDGA
                     VLVVSAADGPMPQTREHILLARQVGVPYIVVYMNKVDMVDDPELLDLVELEVRELLSK
                     YKFPGDEIPIVRGSAKLALEGDKSDMAGGSIPRLMDAIDSYIPTPVRAVDKPFLMPVE
                     DVFSISGRGTVVTGRIERGIVKVGEETEIVGLRDTRKTVVTGVEMFRKLLDEGQAGDN
                     VGCLLRGVDKEEVERGQVLAKPGSITPHKKFVAEVYVLTKEEGGRHTPFFKNYRPQFY
                     FRTTDVTGTVTLKEGTEMVMPGENVRITVELITPIAMEKELRFAIREGGRTVGAGVVT
                     EIIE"
     gene            complement(7364..9436)
                     /gene="fusA"
                     /locus_tag="VJC18_03625"
     CDS             complement(7364..9436)
                     /gene="fusA"
                     /locus_tag="VJC18_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLD44499.1"
                     /translation="MALSKLRNIGIVAHIDAGKTTVTERILFYTGKVHKLGEVHEGTA
                     TMDWMQQEQERGITITSAATTCFWKDQQINIIDTPGHVDFTMEVERSLRVLDGAVGVF
                     CAVGGVEPQSETVWRQADKYHVPRIAFVNKMDRVGADFTGCVQQIREKLGHNPVMIQL
                     PIGSEDQFKGVVDLVSQKALVWHEEDLGAHYETQDIPANLIQDVAQARDALLETIADE
                     DDALMDAYLQGKECSEDLVWKALRSACLKIKCVPVLCGAAFKNKGVQPLLDAVLRLLP
                     SPVDLPAVKGHDLQDPDKIIERKPSSKEPFAALVFKIVSDPYVGHISYFRVYSGRMQV
                     GDRVYNATSDKKERVGRLLLMHANKREEIEDVRTGDIVAAVGLRFTRTGDTLCDEQAP
                     LLLERLEIPEPVINIAIEPKTTVDAEKLAASLDKILIEDPTFRVHTDEESGQIIISGM
                     GELHLEIIVDRLFREFKTAANVGKPQVAYCETLQRETEHELEYSKRIGNQTHYAKVRL
                     RAVPNDAQQGFDFVNLIDHPEFPQAYVKAVQMGVRESLDNGVLAGFPVLGVRVELVAA
                     IFDEENSTELSFKIAASMCFRELCLKADAVLLEPVMDTEVVVPDNFMGEVIADINARR
                     GKVQKMESRRGCQLVRAFVPLAQMFGYATDLRSLTQGRASYTMKFNSYRKVPENVTKV
                     IIEKIRGY"
     gene            complement(9574..10044)
                     /gene="rpsG"
                     /locus_tag="VJC18_03630"
     CDS             complement(9574..10044)
                     /gene="rpsG"
                     /locus_tag="VJC18_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HLD44500.1"
                     /translation="MSRKGQAPKRKILPDPKFKDKVVSKFINRIMWNGKKSTAERAFY
                     QSLEILEKKAGDEGVKVFKQALENAKPLIEVRSRRVGGSNYQVPCEVRAERRVSLAMR
                     WLIGAARGRNEKNIAERLAGEFFDAFRNNGTAIKKREDVHKMAEANRAFAHFKW"
     gene            complement(10136..10507)
                     /gene="rpsL"
                     /locus_tag="VJC18_03635"
     CDS             complement(10136..10507)
                     /gene="rpsL"
                     /locus_tag="VJC18_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HLD44501.1"
                     /translation="MPTINQLIKQGRKSPFYKTASPALQSCPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSH
                     DTQGVEKRRQSRSKYGAKKPK"
     gene            complement(10793..>12425)
                     /locus_tag="VJC18_03640"
     CDS             complement(10793..>12425)
                     /locus_tag="VJC18_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HLD44502.1"
                     /translation="EDVVDAFSGEVLISRGNEVTESVVSRIEEAGIEKVVIRSVLTCE
                     SKRGVCIKCYGRDLSRGHMVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAAAKKAEQ
                     STLEARNDGTIKYNNIKYVVNKDGKAVVMNRHGEIVVIDNTGRERERHPVIYGATLYV
                     KDGSVIKAKQLIADWDPYTVPILTEVDGHILFRDVVEGQSMQEVIDEVTGRSRRIIIE
                     SSNKEHRPRLIIADSKGNPVALGSGATAKYLLPMGTNIVCDDGQEVKAGDVVAKIPRE
                     TTKTKDITGGLPRVAELFEARKPKDCAIISEIDGYVTFGKDTKGKRKVLVTPEVGDAY
                     EYLIPKGKHITVHEGEFVKAGEALMDGSSNPHDILRVLGEKELAKYLTDEVQEVYRLQ
                     GVKINDKHIEVIVRQMLSKVRIIEPGDSAFLIDEPVDRFIFDAENASIKKKKGKLAVA
                     EPLLLGITKASLTTDSFLSAASFQETTKVLTEACISGRVDHLHGLKENVIMGRLIPAG
                     TGIPEYSNVKAQVADYTDETETIAEEEETSEQAIA"
BASE COUNT         3019 a         3460 c         2538 g         3408 t
ORIGIN      
        1 aaggcgtttc aataaatccg tactcattga cacgggcata ggttgaaagt gaagcgatca
       61 aaccgatatt cggaccttcg ggtgtttcaa tgggacaaat acgaccatag tgggtggcat
      121 gcacgtcacg cacttcaaaa ccggcccgat ctctcgtgag accgcctggc cccaacgccg
      181 ataatctgcg tttgtgggtg atttctgaca gcgggtttgt ctggtccatg aactgcgaca
      241 actgtgacga tccaaaaaac tctttcacca cggccgaaac aggttttgca ttgatcaggt
      301 cattcggcat gagcgtttca atttcctgca aactcatgcg ttctttgatg gcccgttcca
      361 tgcgcaccaa accaatgcgg tattgatttt cgagcagttc ccccacggcg cgcacacgtc
      421 tgttacccaa gtgatcaata tcatccaccg tcccaaaacc atctttgaga cgacacagat
      481 atttggtgat gtacaaaata tcttctttgc gcaacacggc acactcgatg ggcacatcaa
      541 aaccaaactt atgattgatc ttgagtcgtc ccaccttcga taaatcgtat cgcccctcgg
      601 taaaaaacag gttggcgaac agcgccttgg ctgcttccag tgtagaggga tcaccaggtc
      661 ttaagcgctt gtatatttct atgaccgctt cgtcggggct gttgactttg tcgaccaaca
      721 acgtgttgcg gatgtaacct cccacgttca gattatcgat gtaaagaatg tcaaatgagg
      781 cgatattggc tttgcgcaga ttttccaaca ctgtggctgt cactgtcgca ttgcactcta
      841 ccaacacttc accggtagat tgatcgatca cgtcatgcgc caccacacaa cccagcactt
      901 cagcgtcttc cagttccaac tcagtcattt tgagttcgtt catgcgtttg atggcacgtg
      961 cagtaaactt tccgtccttc ttcaccaaga cttctttcgt gctgggcact ttgacgtcgc
     1021 gagtcgcacg ttgcaaacgc agaagaccct cattgatctt cttttttaat tttttaccat
     1081 caaaaatgat ggtctcagaa tcataaaagt acttgaggac ctcttcgaca ctcatgtcca
     1141 tagcacgcag gagaatcgtc gcggggatct tgcggcgacg gtcaatacgc acatacagca
     1201 agtctttggt gtcgaattca aaatccagcc aggaaccacg ataaggaatc acgcgagcag
     1261 aatagagaag tttgccagag gcgtgtgtct tgcctttatc gtgctcaaaa aagacacccg
     1321 gggaacgatg taactgagac acgatcacgc gttcaacacc gttgatgata aaggttccat
     1381 tttctgtcat gagcgggatc gtcccgaaat acacttcctg ttccttcaca tcacgaatgg
     1441 tcggagtctc gctaccgatt tcattatccc agacaaccaa gcggaccacc accttcatgg
     1501 gggcctcaaa ggtcatccct ctctggcggc attccaaaac atcgtagcgt ggtttttcga
     1561 aactgtagct cacgaattca agagaggccg tgttgttgaa atcgcgaatg ggaaaaacgc
     1621 tgctgaacac accatgcaac ccaacagcat cgcgcttgtc tgtgggaaca ttgaattgta
     1681 aaaacgtatc ataagatctt ttctggatct cgatcaggtt gggaatttcg actatttttt
     1741 tgatacggga aaaatctttc cgcagacggt agaggtccga aacagacttc gccatgattg
     1801 ctcctttgtc taaaatattc aacaggctgt tgaaaaaccc gttttcgact gcatcatcgt
     1861 caaccactcg tcacacgatc gaacaatctg taacaccttc tcaaaactgg gcaacgcaaa
     1921 cacgagggca atctcccacc ttgctttcaa agcaggaaat tgccctcagg aacactttga
     1981 gtggtctttt tatttgatct caacggtggc accagctttt tccaaagctt ctttgatctt
     2041 ttgagcttca tctttgtcaa cgctttcctt gacgacagat ggcgcgccct caacgaggtc
     2101 cttggcttct ttcaaaccaa gacctgtgat cgtacggatt tctttgatca cgttgatttt
     2161 gttggcacca atttccttga gggaaacatt gaattcggtc ttttcttctg cagcagcggc
     2221 aacagcggct ccgccggcaa cagcaacggc cattggggca gctgctgaca caccaaattt
     2281 ttcttcaaac gcttttacca gatcggcgac ttccaatacg gtcatcttcg atacggcctc
     2341 gacaatttct tgtgttgtca tacaaaacga ttcttgaaac ctgtccttgt caaacggctt
     2401 ccgttgacag caaaacattt ttcaaaaaaa tctcttttag actgattgtt ctttttgtcc
     2461 cttcacggaa ttgagcacag taaccagttg tcttgggatt tgacagagca cgctcaccaa
     2521 attcgtggca ggcgcgttca atgatcccaa caacttcgca atgagctctt ccttactcgg
     2581 cagagatgcg agattcttta ttccgttgct gtccagatat ttgccattca gtaatcccac
     2641 cttgatttta aggcgttcat tcttcttggc aaactcctcc agcaccttgg cagggccggt
     2701 cggatcagac tgggaaatcg tgatcgctgt gttgcccaca aaagagtcac ccagcacaga
     2761 agcggctgtg tctttcacgg caattttggc taaacgattt ttgacaactt tgaaaaatga
     2821 gtttttggct ctaagttgtc ttctgagatc cgtcagagcg gcaacgggca gaccctgata
     2881 gtctgcaaga atcgtcaact gagacttggc caagcagtct ctgatttcat ctatgtgagc
     2941 tgccttttca gtgcgattca tgacttgtct cctttcgaga cttcattgac gtcgatacgt
     3001 gccaagccca aacaatcagg ctatcaccgt ctgtgatgga ttgaacactc attttaaaaa
     3061 cgaggcgtac cccattttcc atcagtcttc gacgaaaatg aaacttgttc attcaccaac
     3121 caaaccgaaa atatccgact gcggttcggt tggtcaggtt gccctagcga acaaccccac
     3181 tcaaccagca actttcgaaa cttcgttcac atcgactgcc acaccaggcc ccatcgtgga
     3241 agacagggtg atgttcttga ggtaaacgcc ttttgaggtc gccggcttca ttttacgaat
     3301 tgtttccaat aagactttta aattatctgt gagtttttct gcgccgaagc tgatcttgcc
     3361 aaacggggca tggacgttcc ccgctttttc attgcggaac tcgaccttcc cagccttgca
     3421 atcctggatg gcctttttca cgtcgaaact caccgtgccc aacttggggt tcggcatgag
     3481 accacgaggg cccaaaatct tgccaacctt actcacaaca cccatcatat cgggagtggc
     3541 taccacggaa tcaaagtcga gccaaccacc ctggattttc tcgatcatgt cggtgttgcc
     3601 cacaaaatcg gcccccgcct ctttggcttc tgtttccttc tcacccttca cgagagccaa
     3661 cacgcgaacc ttcttgccca gaccatgcgg caacacaact gatccgcgaa ttccctgatc
     3721 tgattttttg gcatcaatcc ccaaccggac acacacatca actgtctcat caaatttgcg
     3781 tgttgcccct tgcagcacca acgccatggc gtcagccaag gcatactttt tttctgaatt
     3841 aatttttttt ctgatttctt tttgtttttt tggaattttc ataagcacct catgcaacaa
     3901 cttcgatacc catgctcttg gctgttccct tgaccgtatt gatggccgca tccagggtaa
     3961 aacagtttaa gtcaggcatc ttcaccttgg caatatcctc aacctgtttc agggtcacct
     4021 tgccaaccaa agatttacct gtatcaccag cacctttttt tacactggcg gccttcatca
     4081 gaaggataga tgcggggggg gtcttgagga caaaactgaa agagcggtct gagtacactg
     4141 tgatcacgca aggggtaatc atcccgacct ctttttgtgt cgccgcgttg aacttcttac
     4201 agaactccat gatgttcaca ccatgttgcc ccaacgcagg accgacagga ggagctggat
     4261 tcgcctgccc cgctggacat aacaacttta tttgtgcgat tggttttcgt gccataattg
     4321 ttgccgtatc tcgttattcg tgaagcgtat ctcgtaaaga aaataactct cttcacgttt
     4381 cacgtttcac gcttcacgaa atacgctagt ttttctccac ctgcgtgaat tccagatcca
     4441 ctggggtggc cctgccaaaa atgctgacga gcaccttcag ccgacccttc tcgggattca
     4501 cttcttcgac aataccatta aacgtattga aaggaccatc tgtgactctg acagaatcgc
     4561 ctttttcaaa attgtacttc agtttgggct tcaattcgcc actatcaatc tgctgcgtga
     4621 tcttgagcac atcttcttcc gggattgacg gtggactttg cgagtttccc acaaacccgg
     4681 tgatcttcgg cgtgtccttc accaaatgcc acgtttcatc ggtaagatcc attttcacca
     4741 ggatatagct gggaaaaaac tttctggtcg tggtcttttt ttgtcccttg cgaatctcca
     4801 caacactctc tgagggcacc agtatttcct caaaatgctc ctgcatttga gttgacttaa
     4861 cacgttcttc gagggacagt tttgcttttt gttcaaaccc tgcataggta tgaacaacgt
     4921 accacttttt agacatgtga tttcccgcca tctaaacaat agattagtta agtatccatc
     4981 tggtcagcgt ggaccaaaat aaatcgtata aagccaacat ggttgtcgca atgccgacta
     5041 gaatcaccac gacaacagct gattgaccag attcctttgt cgtagggtag gtcactttcg
     5101 acaattctgt cacagcttcc tcgaggaagc tcatgaatct ctgatgcctc aacgtcaacg
     5161 caaacgccac accagcacaa acagcaccaa tcaaatccgg tatggtgaca aaataatcgc
     5221 gtgtgatcgc caagttgaac caatcgaaca gcagttcaca aatttgagag gcgacccaat
     5281 acaaaaaaac ggctacaaga atcaaacaaa tagtaacaat ttttctattt ttcatatgac
     5341 cgtcccctac aacaagagca atttattctg taaaaaccat taataaattg attttattgg
     5401 ttttttattc gtgggttttt tgcagtcgct tgactactct atttattcat gaaaataaaa
     5461 gaaaactcgt cattttcttt aacgcattgc atcattacac aagcaggagt ggcagggttc
     5521 gaacctgcga cctacggttt tggagaccgt tgctcttcca gctgagctac actcctaaaa
     5581 tttgcttcgc aaatttggtt attcgtagtt agtcgttcgt agtttaaaaa ccagaggaac
     5641 cagaaattgc ttcgcaaatt tcgttttttg taattagtaa tatcacaaat cgatccacga
     5701 gccattcatc caccgaagcc ttggctcagg cggacgaacc acaaatcaca atattcacct
     5761 cacttgcaca tgcatggtgt gggccttgca atatttgcaa aactttttta gcttcaattt
     5821 tttatctttc ttatccttgt taatcttaaa gcggtagttt cgttttttac aaacctcaca
     5881 ttcgagcgaa actgtcacta ttaaattcat aaagaaataa aaggcgaccc cttctcagag
     5941 accgccttcc caaatttcgt ctttcgtgga tcgtggatcg tgaatcgtaa atcgcctgtg
     6001 ccctttaggg tatgaatcga atttttcgat ccacgattca cgattctcga ctcacgtttt
     6061 ctactctata atctccgtca ccacgccagc gcccacggtt ctgccacctt cacgaatggc
     6121 gaagcgcaat tctttttcca tcgcgatcgg cgtgatcaat tctaccgtga tccgcacatt
     6181 ctcgccgggc atcaccattt ctgtcccctc tttgagcgtc acggtccccg tcacgtccgt
     6241 ggtcctgaaa taaaactggg gccggtagtt cttgaaaaag ggcgtgtgcc gtccgccttc
     6301 ttccttcgtc aacacataca cctccgccac aaacttcttg tgcggcgtga tcgatcccgg
     6361 cttcgccaac acctgcccac gttccacttc ttctttgtcc acacctctga gcaagcaccc
     6421 tacgttatca cctgcctgcc cttcgtccaa caacttgcgg aacatttcca cgcccgtcac
     6481 cactgtcttg cgggtatcgc gcaaacccac gatctccgtc tcttcgccta ccttcacaat
     6541 gccgcgctcg attctgcccg tcaccactgt cccgcgaccg gagatgctga acacgtcttc
     6601 gaccggcatc aaaaagggct tgtcgacagc gcggaccggg gtcgggatgt agctgtcgat
     6661 cgcgtccatc aaccgaggga ttgaccctcc ggccatgtcg ctcttgtcgc cttccaaggc
     6721 cagcttcgcg ctcccgcgta cgatcggaat ctcatcacca ggaaatttgt acttgctcaa
     6781 aagttcgcgc acttccaact caaccagatc cagcaactcg ggatcgtcca ccatatccac
     6841 tttgttcatg tacaccacta tgtacggaac acccacctgg cgggccaaca aaatgtgttc
     6901 acgggtctgg ggcatgggac catctgccgc gctcaccacc agaacagctc cgtccatctg
     6961 cgccgctccg gtgatcatgt tcttgatgta gtccgcatgt ccggggcaat ccacgtgcgc
     7021 gtaatgccgc gtgtctgtct ggtattccac gtgcgccgta ttgatcgtga tcccgcgttc
     7081 tttttcttcg ggggctttgt cgatttgatc ataggccacg aaggtggccc tgcctttgac
     7141 cgccaacacc agggtgatcg ccgccgtcaa cgtcgtcttg ccatggtcca cgtgcccgat
     7201 cgtccctatg ttcacatgcg gtttcgtccg ttcaaatttt tcctttgcca tatttttctc
     7261 cttaattcgt gggtcgtggt tcgtggatcg cttgtgccct tgagggcatg aatcgtgaat
     7321 gcaactttcg atccacgatc aacgatcctc gattcacgat tttttaatac cctctaattt
     7381 tctcaataat cacctttgtg acattttcag gcaccttgcg gtagctgtta aacttcattg
     7441 tgtaagatgc cctgccctgt gtcagcgacc tcaaatctgt cgcgtaccca aacatctgag
     7501 ccaaagggac aaaagccctc accaactgac aacccctgcg actctccatc ttctgtactt
     7561 ttcctcgacg cgcgttgatg tcggcgatca cttcgcccat aaaattatct gggaccacga
     7621 cctccgtatc catgactggc tccagcaaca cggcatctgc cttcaaacac aattctcgaa
     7681 aacacattga ggccgcaatt ttaaaagaca gctcagtact gttttcttca tcgaagattg
     7741 ccgccacaag ttcaacccga acacccaaca cgggaaaccc cgccaaaaca ccattatcca
     7801 gtgattcccg cacgcccatc tgcacagcct tcacgtatgc ctgtggaaac tccggatgat
     7861 ctatcagatt cacaaaatca aatccttgtt gcgcgtcatt tggtactgcc ctcaacctca
     7921 ccttggcata atgtgtctga ttgcctatcc gtttgctgta ttccaactca tgctcagttt
     7981 ctctctgaag cgtctcacag tacgccactt gcggttttcc gacattagcc gcagttttaa
     8041 actctcggaa caagcggtcc acaatgattt cgagatgaag ttcccccatc cccgaaatga
     8101 tgatttggcc tgattcctcg tcggtgtgca cacgaaacgt ggggtcctca atcaagattt
     8161 tatccaaaga agccgcgagc ttttcagcat cgaccgttgt ctttggttca atcgcaatat
     8221 tgatcacagg ttccggaatc tccaaccgtt ccagtaacaa cggagcctgt tcatcacaaa
     8281 gcgtgtcccc cgtcctcgta aacctcagtc ccaccgcagc cacaatatca cccgtacgca
     8341 catcttcgat ttcttctctt ttgttagcat gcatgagcag aagtcgacca accctctctt
     8401 tcttgtccga cgtcgcgtta tacacgcggt caccgacctg catacgcccg gaatagactc
     8461 gaaagtagct gatgtgcccc acataggggt ccgacacgat cttgaaaacc agagcagcaa
     8521 aaggttcttt tgatgaaggc ttcctctcaa tgatcttgtc gggatcctgc aaatcgtgac
     8581 ccttcacggc tggcagatca accggcgatg gcaacagcct cagcacggca tcaagcaatg
     8641 gttgcactcc tttgtttttg aaggcggcgc cgcacaaaac cggcacgcat ttgatcttga
     8701 gacaagctga tctgagcgcc ttccaaacga ggtcttcaga acactccttg ccctgcaaat
     8761 aggcatccat cagtgcgtca tcttcatctg cgatcgtctc caacaaagcg tcccgtgcct
     8821 gcgcaacatc ctgtatcaga ttcgctggga tgtcttgtgt ctcgtaatga gcccccaaat
     8881 cctcctcatg ccagaccaag gccttctgac tcaccaggtc caccacaccc ttgaattgat
     8941 cctcagaacc aatagggagc tgaatcatca cagggttgtg tcccaatttt tcccggattt
     9001 gttgcacaca acccgtaaaa tcggcaccaa cacgatccat cttgttgaca aaggcaatgc
     9061 gcggcacgtg atacttatct gcctgacgcc agacggtttc gctttgaggt tcaaccccac
     9121 caaccgcaca aaaaacgcca accgcaccat ccagcacacg caaactgcgt tcgacctcca
     9181 tcgtgaagtc aacatgacca ggcgtatcga tgatgttgat ctgctgatcc ttccagaaac
     9241 aagtcgtcgc ggctgacgtg atcgtgatcc ctctctcctg ctcttgctgc atccagtcca
     9301 tcgtggcagt tccttcgtgc acctcaccca atttgtgcac cttgccggtg taaaacagga
     9361 tcctctcagt caccgtcgtc tttcccgcat caatgtgagc gacaatacca atatttctca
     9421 attttgaaag agccattttt gtatcgcgtt ccgccataac cttgatcgcg atgcgcagca
     9481 cgccacgcgt cctgcgcttc ctatcaaaaa ctgcttcagg cagccagtga cagagaccac
     9541 gcacagcgca tcgcgcactg cgcacggctt atattaccat ttaaagtgag caaacgccct
     9601 gttggcttcg gccatcttat gaacatcttc tcttttcttg atggctgtcc cattattcct
     9661 gaaggcgtca aaaaattctc ctgccaaacg ttccgcaatg ttcttttcgt tgcgaccacg
     9721 agccgcgcca atgagccacc gcatggccaa cgacacccgt ctctctgccc tgacttcgca
     9781 gggaacctga tagttggaac caccaacacg ccgtgaacgc acctctatca acggcttggc
     9841 gttctcgagt gcttgtttaa aaaccttaac gccttcatca ccggccttct tctcaagaat
     9901 ttcgagactc tggtagaaag ctctttcagc agtgctcttt tttccattcc acatgatccg
     9961 attgatgaac ttactcacca ctttatcttt gaacttggga tctggtaaaa tttttctttt
    10021 cggtgcttgg cctttacgag acataaatca ttcaattgta gatttttggg ttcgaatttt
    10081 gtttgatcct ggcacaggac aattcaaaac cctagaaaaa aaactacaac cttcattact
    10141 tcggtttttt cgctccgtat ttggaacgac tttgacgacg tttctccaca ccctgtgtgt
    10201 cgtgcgaacc ccgaacaatg tgataccgca caccgggcaa atccttgaca cgaccgcctc
    10261 ggatcaacac aaccgaatgt tcctgcaaat tatgaccttc accaggaatg tatgatgtaa
    10321 cttcaatccc attggttaaa cgaacgcgag ccacttttct gagggccgag ttgggtttct
    10381 tgggtgtcgt tgtgtaaaca cgaacacaaa ccccgcggcg ttgcggacaa gactgcaacg
    10441 ctggtgaggc tgttttataa aacggacttt ttcttccctg cttaatcagc tgattaatgg
    10501 tcggcatagc cacctacctc tttcaacata ttgatattat taatgttata attataaaac
    10561 tcttctcttc acaaagaagg cgcgcaattt agcaagccac accccgcttg tcaagtgacc
    10621 taaaaataaa tctactgttg agatcaaatt tagtctatgt gcttgttttt actaagcttc
    10681 ttagacagct ttttgatcta ttccaaaaag ccaaaaccaa gatggttagt caaaccatct
    10741 tacgacaggt aaaaccatcg tcggctgggg ctccatatcc taaaaaatat ctttatgcaa
    10801 tagcctgttc gctagtttct tcctcttcgg ctattgtctc tgtttcatca gtataatcag
    10861 caacttgagc cttcacattg ctgtattctg ggatgcccgt tccggctgga atcaaacgac
    10921 ccataatgac gttttctttt aagccatgca ggtgatcaac tcgccccgaa atacacgctt
    10981 ccgtgagcac ttttgttgtt tcctggaagg aggcagcaga caagaaactg tccgtcgtca
    11041 aagaggcctt ggtgatcccc aagagcagtg gttctgcaac agccaacttg cccttcttct
    11101 ttttgatcga cgcgttttcg gcatcgaaaa taaaacgatc aacaggctca tcgatcagga
    11161 aagctgaatc tcctggttca ataatacgaa ccttggacag catctgacgc acaatgacct
    11221 cgatgtgttt atcattgatc ttcacaccct gcaatcgata cacttcctgt acttcgtctg
    11281 tgaggtactt ggccagttcc ttttctccca gcacacgcaa gatatcgtgt gggttggaag
    11341 aaccatccat gagcgcctcg cccgctttga cgaattcacc ttcatggact gtgatgtgct
    11401 tccctttggg gatgaggtat tcgtaagcat cacccacttc gggagtcacc aggaccttcc
    11461 tcttgccctt cgtgtctttg ccaaaggtca cataaccatc aatttccgaa atgatcgcac
    11521 aatcttttgg ttttctcgct tcaaaaagtt cagcgactct gggcaaaccg cctgtgatgt
    11581 cttttgtttt cgtggtttct ctcgggatct tggccacgac atcaccagcc ttgacttcct
    11641 gtccatcgtc acaaactatg tttgtgccca taggcaaaag atacttcgca gtcgctccgg
    11701 agcccagcgc aaccggattt cccttgctat cagcaatgat gagtctggga cgatgttctt
    11761 tattgcttga ctcgataatg atacgacgtg aacgcccagt cacttcgtca atcacctctt
    11821 gcatggactg gccttcgacc acatcacgga acaagatatg cccgtcaact tcagtcaaga
    11881 tgggtaccgt gtaaggatcc caatcagcga tcagctgttt ggcttttatc acgctaccat
    11941 ctttgacgta caaggtcgca ccataaataa cagggtggcg ttcacgttca cgaccggtgt
    12001 tgtcaatcac aacaatttcc ccgtggcgat tcatcacaac agccttgccg tccttgttca
    12061 ccacatactt tatgttgttg tatttgatgg taccatcatt gcgagcttcc aacgtggatt
    12121 gttcagcctt tttcgcggca gcccccccaa tgtgaaaggt tctcatcgtc agctgtgtgc
    12181 cgggctcacc aatgctctgc gcggcaatca cgcccactgc ttccccgatg ttgaccatgt
    12241 gtccacgtga caaatcgcgt ccataacact tgatgcaaac accgcgtttg ctctcacaag
    12301 tgagcacgga ccggatcacc actttttcga tccccgcttc ttcaatcctg ctcaccaccg
    12361 actcggtcac ttcattgcct ctgctgatca gcacttcacc agaaaaagca tcaacaacat
    12421 cttcg
//
LOCUS       DATDUN010000265         2116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_347576, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000265 DATDUN010000000
VERSION     DATDUN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347576"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..>2116)
                     /locus_tag="VJC18_03645"
     CDS             complement(76..>2116)
                     /locus_tag="VJC18_03645"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HLD44503.1"
                     /translation="ERAFYKAMRTGGLHRNTNNRITIGAVVDYEDEWYEYNEALIRTL
                     SVVAQKDEAGLERLVRKNIKSQAGELVRKLRALFEKFYTVTPGNIRELVYEHVEKHFD
                     GDVPEQDAFEEMEGIGFETSSWDDVEGQDDDYDADVDSEGNSSGDKAEQTDDLSVGKK
                     LWLAVLNRWEVDLDEFCEELMLFFDRHALSRMAKLANEHVVRMLPGVSCKRDDSREWS
                     ERLFSTIRDEYGVQSSGQAKKALRILEDELPKEFRTRALRRDSVHLHQAEMPAVFKGR
                     PPLVEFLAMRYYLELPPGEQKELVRQLDTLGAEESEGALIRRFFELTVVKVGQFLSIW
                     PEVPQQHRSALEGLQDDVPRGDFQDVKDTIKAEFPLNEANALIKRIEPEPLNVGTIGE
                     VYRVKIGDGKYRVLKVIPASKRAKVELTLRRLRHIYFVLDNYREKVSGASTAMRVISQ
                     LIQSIEKELDLRKERSDALAFGQQLRNYQGGKYSQHVSIPLYSKDYCSRSVLLMSHER
                     AEKITRVTDGALAQKVAKYLDDMLNDMIANAEMYPDDLHPGNILVEKGEEGNPDSWGV
                     VLLDFGRMGRTTPEQRSLVMRFALAAIMKHQESLQKVIEEMSGPSALSDQYDSSAFAS
                     EMSQLINDGTTEDPYELLSHLLTLAGKHHLEIGLPFIQILKAKMTMRGTLHAVGV"
BASE COUNT          526 a          604 c          418 g          568 t
ORIGIN      
        1 ttcaactcat aaaatccgaa gggattataa acgcaggggc tttggttaaa cgaccactgg
       61 caacaccagg gaaggctaca ccccaacagc gtgcaacgtg cccctcatgg tcatttttgc
      121 cttcaggatt tgaataaaag gcaatccaat ttccaggtga tgctttcctg ccagcgtgag
      181 caaatgagac aagagctcat agggatcttc tgttgtgccg tcatttatca actgagacat
      241 ctccgaggca aaggcactgg aatcatactg gtccgataac gctgagggcc cactcatctc
      301 ctctataacc ttttgtagcg attcctgatg cttcattatg gccgccagag caaaacgcat
      361 gaccagagac ctttgctcag gtgttgtccg tcccatgcgg ccaaaatcca ggagcaccac
      421 tccccaactg tctgggttac cctcttcacc cttctctaca agaatgtttc cgggatgaag
      481 atcatcggga tacatttcgg cattcgcaat catatcattg agcatgtcat caagatactt
      541 tgcgactttt tgcgccaaag ctccatccgt tacacgtgtt atcttttcgg ctctttcatg
      601 gctcatgagc aacacggatc gtgaacaata atccttcgaa tacagtggga tgctcacatg
      661 ctgactgtac ttccctccct ggtaatttct caattgctgt ccaaacgcca gggcatcact
      721 gcgctctttc cgaagatcca attctttttc tatggattgg atgagctgtg aaatcacgcg
      781 catggctgtg gaggctccgg aaactttttc ccgataatta tccaaaacaa aatagatatg
      841 ccgtaaccgt ctcagcgtca gctcaacctt ggcccgttta gacgccggga tcactttcaa
      901 aacacggtat ttgccgtcac caatcttgac cctgtacact tcaccaatgg tgcccacgtt
      961 tagcggttca ggctcaatgc gtttgatcag cgcgttggcc tcattcagag ggaattctgc
     1021 cttgatcgtg tccttgacat cctgaaaatc accacgaggc acatcgtctt gcaggccttc
     1081 caaggcactg cggtgctgtt ggggaacctc tggccaaata gacagaaact gtcccacctt
     1141 gacgactgtc agctcaaaaa aacgtcgtat caacgcccct tctgattcct cagcgccaag
     1201 cgtatccagt tgtctcacca gttccttttg ctcaccgggc ggcaattcca gataatagcg
     1261 cattgccaga aactcaacaa gcggtggcct gcccttgaat acagcaggca tttctgcctg
     1321 atgcagatga acactgtctc gccgcaaggc acgcgtcctg aattctttag gcaattcatc
     1381 ctcaagaatt cttaacgctt ttttcgcctg tccacttgat tgcaccccat attcatcccg
     1441 aatagtcgaa aacagtcttt cactccactc acgggagtca tcacgcttac aggaaacacc
     1501 aggcaacatt cggaccacat gctcgttggc aagttttgcc attcgagaaa gtgcgtgtcg
     1561 atcaaaaaac aacatgagtt cttcgcaaaa ctcatccaga tcaacctccc aacgattgag
     1621 aacggccagc cacagttttt ttccaacact caaatcatca gtttgttcag ccttgtcccc
     1681 cgaagaattt ccttcagaat ccacatccgc atcatagtca tcatcctgcc cctcgacatc
     1741 atcccaagaa gatgtttcaa aaccgatccc ctccatttcc tcaaaggcat cctgctcagg
     1801 gacgtcgcca tcaaaatgtt tttccacatg ttcatacaca agctcacgaa tattcccggg
     1861 agtgactgta taaaattttt caaagagtgc cctcagtttc cgaaccagtt ccccagcttg
     1921 acttttaata ttctttctca caagtctttc gagcccggct tcgtctttct gagccaccac
     1981 acttaaggtc cggatcaagg cttcattgta ttcataccac tcatcctcat aatcaaccac
     2041 ggcgcctatc gtgattcggt tgttggtgtt tctgtgtagt ccccctgttc tcattgcctt
     2101 gtagaaggcc ctttct
//
LOCUS       DATDUN010000266         1714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_138476, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000266 DATDUN010000000
VERSION     DATDUN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138476"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VJC18_03650"
     CDS             complement(<1..194)
                     /locus_tag="VJC18_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007018647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HLD44504.1"
                     /translation="MSASNNIKVASLDGRMEYFKQGGKDVFAKAQDFIEADMIRQLNI
                     YPYYQTIDSNEGPLCIIEGK"
     gene            478..>1714
                     /locus_tag="VJC18_03655"
     CDS             478..>1714
                     /locus_tag="VJC18_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HLD44505.1"
                     /translation="MTTKHVLLRAVFFTITLAAVVLISACGGEKLTYTDTPGDGDIIV
                     PPAGGGGTSTDDVIDTDSDGISDENDNCVYYMNTDQADFDADGDGDECDIDDDNDGTK
                     DKSDVCPNDANGHDDPDDDGLCGTSDNCEDVANEDQADADADGIGDVCDDDADNDEIK
                     DDADNCPEISNADQADLDADTVGDVCDDDKDDDTILNDVDNCEIVSNEDQTDSDGDGE
                     GDVCDGDTDGDLIANEDDNCPTVANADQTDEDGDLVGDVCDNCFDVENTDQADLDADT
                     LGDVCDSDADGDGETNDTDCDDLDASSHNGALEVFDFADNDCEGTVDQGISLDNAVFS
                     ITGEASGNYFGYRAVVLSDVNGDGIDDVLASAYKNSEGGTSAGKVYLIYGAGNLSGDL
                     SAENANVTFVGEAANDYLGL"
BASE COUNT          463 a          369 c          459 g          423 t
ORIGIN      
        1 tttttgccct cgataataca gaggggacct tcgttggaat caatcgtctg ataataggga
       61 taaatattga gttggcgaat catgtcggct tcgatgaagt cctgcgcctt ggcaaaaaca
      121 tctttcccac cctgtttgaa atattccatc cgaccgtcga gagaggccac cttgatattg
      181 tttgacgcgc tcataatttc tgcactcctg tttttcctgt actggacatg taatgagggc
      241 cgtatttaac cacaaacaac aagaaatgca aattttgcca aataagacac tcaaaaaccc
      301 gtcttgtaac aacttgacta tggacttgta ataattggtt attttacctt tatgtcttgt
      361 cgcacaaaac atttgttaaa aagacgaaac ttttgcctca aattgtcgat aaatgtcctg
      421 aaaagggtag cgttgtgttt caaaccgggt cgttgtgatg acatgattgg ggtaattatg
      481 acaacaaaac atgttttatt gagagccgtg ttttttacca tcactttggc agctgtcgta
      541 ttgatcagcg cctgcggtgg cgaaaaactg acatacacag acacgcctgg cgatggcgac
      601 atcattgttc cgcccgctgg tggtggtggc acgtcaacgg acgatgtcat cgatacagac
      661 agcgatggta tcagcgacga aaatgacaac tgtgtttatt acatgaacac cgaccaagcc
      721 gacttcgatg ccgatggcga cggagatgaa tgtgatattg atgatgacaa cgacggcacc
      781 aaagacaaaa gcgatgtctg ccctaacgac gccaatgggc atgatgatcc cgatgatgac
      841 ggcctttgcg gaacgagtga caattgtgaa gacgtcgcca atgaggatca ggccgatgcc
      901 gacgcagacg gcattggtga tgtgtgcgat gatgatgccg ataacgatga aatcaaagat
      961 gatgccgaca attgtcccga aatcagcaat gctgatcagg ccgatctgga tgctgacact
     1021 gtgggcgatg tttgtgacga tgacaaagat gatgacacta ttttaaacga cgtcgacaat
     1081 tgcgaaattg tgtccaacga ggatcaaacc gattctgatg gcgatggcga aggtgatgtg
     1141 tgcgatggtg ataccgatgg agatcttatc gccaacgaag atgacaactg ccctacagtg
     1201 gccaacgctg accaaaccga tgaagatggt gatctggttg gtgatgtgtg cgataactgt
     1261 tttgacgtgg aaaataccga ccaagcggac cttgacgcag acaccctagg ggatgtctgt
     1321 gacagcgatg ccgatggaga cggcgaaacc aacgacacag attgcgatga cctcgatgcc
     1381 agctcacata acggggcatt ggaggtgttt gactttgccg acaacgattg cgaaggcacc
     1441 gtcgatcagg ggatcagcct ggacaatgcg gtgttttcaa tcactggaga ggcttcgggt
     1501 aactattttg gatatcgcgc cgtcgtctta agcgacgtga acggcgatgg gatcgatgat
     1561 gtgctggcga gtgcctacaa aaattctgag ggtggcacct cggcgggcaa ggtatatttg
     1621 atctatggcg cgggaaattt gtcgggtgac ctcagcgccg aaaacgccaa cgtgactttt
     1681 gttggcgagg ccgccaatga ttatcttggc ttgg
//
LOCUS       DATDUN010000267         1422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_219772, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000267 DATDUN010000000
VERSION     DATDUN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219772"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VJC18_03660"
     CDS             <1..473
                     /locus_tag="VJC18_03660"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLD44506.1"
                     /translation="GLVTLDLGIRHAKEIAGLVTVAAALKFADPLAGFTHILSKAIKF
                     WPSPNAFQDKTLAKKSTNYQKFATDAFGSLFDYAKEIEAKLSQVKVPLRVLHSKKDQV
                     IAPISANIIYEKVSSKTREIVWFKESGHEMMQDLESPAVFDAIMEFVLKFKKQI"
     gene            578..961
                     /locus_tag="VJC18_03665"
     CDS             578..961
                     /locus_tag="VJC18_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44507.1"
                     /translation="MKKLILGFTVLALVLWAGASFAASLDGNYSFVSRAKANQPDLVG
                     WTGTMSITQGTVTRNLTSPDGKEVKYYDNTLTDNGNDLYTFKTTRAYKPDYVGQEYQN
                     KVTLKDNQLIIASPDNTFNEVWAKK"
     gene            complement(1048..>1422)
                     /locus_tag="VJC18_03670"
     CDS             complement(1048..>1422)
                     /locus_tag="VJC18_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HLD44508.1"
                     /translation="WPYHSVPHKDKPSTWVPFKKGMCALCWATCCTMPIEMCFEDVVR
                     LGWVFGDEDPRFAAKRLLKEGKIDRYRDKAGVFLLARKSNHACILLGEDRQCTEYNKR
                     PATCQNFPKVSSRPGYCPAVKK"
BASE COUNT          378 a          396 c          321 g          327 t
ORIGIN      
        1 gagggctggt cacactcgat ctgggtatcc gccacgccaa ggaaattgcc ggcctggtga
       61 ctgtggccgc cgcgctcaag tttgccgacc cgctcgccgg gtttacacac atcctgtcca
      121 aggccatcaa attctggccc agcccaaacg cctttcagga caaaacactg gccaagaaaa
      181 gcaccaatta tcaaaaattt gccacagacg cctttggatc cctgttcgac tatgccaagg
      241 aaatcgaagc aaaactgtca caggtcaaag tccccctccg tgttctgcat tccaaaaagg
      301 accaggtcat cgcgcccatc agcgccaaca tcatttatga aaaagtgtct tctaaaaccc
      361 gcgagatcgt ttggttcaaa gaatccggcc atgagatgat gcaagattta gaatcgccag
      421 ccgtgttcga cgccatcatg gaatttgtgc tgaaatttaa gaaacaaatt tgacaaatca
      481 cacccatacg ttatgcacgc tgtgttgaca ggcagtaaca ttaacgcttt gcccccagtc
      541 tcgcctcgcg tgatttgggg cacggcaagg agtcattatg aaaaaattaa ttcttggttt
      601 taccgttttg gcactggttc tctgggcagg ggcctcattt gccgccagcc tcgatgggaa
      661 ctattcgttt gtgtcacgtg ccaaagccaa tcaacctgat ctggtcggtt ggaccggtac
      721 catgagcatc acccagggca ccgtcacacg taacctcaca tcgcccgatg gcaaagaggt
      781 gaagtattat gacaacaccc tgaccgacaa cggcaatgat ctttatacct tcaaaaccac
      841 acgcgcctac aaaccagatt atgttggcca ggaatatcaa aacaaggtca cattgaaaga
      901 caatcaactg atcatcgcaa gccccgacaa caccttcaat gaagtgtggg caaaaaaata
      961 attttgtgtc attgcgagtc cttcctcacc ccaatcatga tatcggtgag gaaggacgtg
     1021 gcaatccacg ttcttacaat ttctgattca ttttttcacc gcagggcaat aacccggtcg
     1081 tgaactcacc ttgggaaagt tctggcaggt ggcaggacgc ttgttgtact cggtgcactg
     1141 cctgtcttct cccagcaaaa tgcaggcatg attgcttttg cgggcaagca ggaacacacc
     1201 agccttgtca cgataacggt ctatcttccc ctctttcaac agcctcttgg ccgcaaaccg
     1261 agggtcttcg tccccaaaga cccaccccaa acgcacgaca tcctcaaaac acatttcgat
     1321 gggcatggtg caacacgtgg cccagcacag cgcgcacatc ccttttttga acggcaccca
     1381 agtggatggt ttgtctttgt gggggacaga gtgatatggc ca
//
LOCUS       DATDUN010000268         2863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_14626, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000268 DATDUN010000000
VERSION     DATDUN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14626"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1419
                     /locus_tag="VJC18_03675"
     CDS             103..1419
                     /locus_tag="VJC18_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HLD44509.1"
                     /translation="MTQQRVSIKPATGKLGVLLVGLGAVATTFVAGVEAAKKGVRKPI
                     GSLTQIGHIRLGKRTENRNPLVKDVVPLADINDLVYGAWDLFPDNGYEAACKAGVIPM
                     DLLTKCCDDLKNILPMKAVYDRNYCRRLEATHVKSETNKMAQAEALIKDIAEFKTKNN
                     CDRLTMIWCASTEAFNTVTEAHKSVHALEEGMRNNDPTISPSMIYAYAALKSRIGYAN
                     GAPNLTTEIPALQELAKEMRVPLSGKDFKTGQTLMKTIVAPGLAARYLGMTGWFSTNI
                     LGNRDGEVLDDPQNFKTKEESKLSVLDSILNPELYPDLYGDLYHKVRIEYYPPRRDNK
                     EGWDNIDLVGWLGMPMQLKINFLCRDSILAAPIVLDLVLFMDLAQRAELKGIQEWLSF
                     FYKSPMVKEGLTPIHNLFSQEMKLQNTLRYLIGEEQITFLGLDYYE"
     gene            1519..1863
                     /locus_tag="VJC18_03680"
     CDS             1519..1863
                     /locus_tag="VJC18_03680"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HLD44510.1"
                     /translation="MNRTQRQSFSSIGSLLKKKLAGPEHEPLQKTVALHQQWLKIVGP
                     IISRHSRILYIKDGCLFVSVAHPAWHNELSLMKNQILKQIQEQLPDTKVTSIKFKTDA
                     ESQYEKGTKRTF"
     gene            1835..>2863
                     /locus_tag="VJC18_03685"
     CDS             1835..>2863
                     /locus_tag="VJC18_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLD44511.1"
                     /translation="MKKEQKGPFNKILEVNLTKQIFTVCTVTKKDRVMYLGAKGLALK
                     LLFDRVKPGVDPLGPDNYLAFFMGVVMGTGAPCSGRFSSVTKSPLTGIFTHSSCGGPF
                     GMQLKTAGWDGVLITGAAKKPTYLEITESGVAFKDASSLWGLDTVETQKRFGDKGSAL
                     VIGPAGENGVLFANIASGHRFLGRGGAGTVMGAKNLKAILAIGDVYKILPINQKDFDQ
                     VKKQATHYINSNDVTSDKYRNYGTSANVRLCNKAGILPVNNFTKGQHEDADNISGETV
                     KERFNTKHATCRPCTILCGKKGTFEGQELIVSEYETVGLLGSNLGIFDPIKIAHWNKL
                     CGDMGMDTI"
BASE COUNT          782 a          770 c          666 g          645 t
ORIGIN      
        1 cttttttgca agtggttcga ttaacatatt ggatttcttc tccgactctg cttgatgcaa
       61 tacacgaatc ggatggagag aaaccagaca ggaaggaata atatgactca acagcgtgtt
      121 tccataaaac cggctacggg aaaattgggt gttctccttg ttggcttggg tgccgttgcc
      181 acaacctttg tcgctggtgt cgaagccgct aaaaaaggtg tgagaaaacc tatcggttcc
      241 ctcacccaaa tcggacatat cagactgggc aaacgaacag aaaatcgcaa tcccctcgta
      301 aaggatgtgg ttcctctggc cgacatcaac gacctggtgt acggtgcctg ggacctattc
      361 ccagacaacg gttacgaagc cgcttgcaag gccggtgtga tccccatgga cctgctcaca
      421 aaatgctgtg acgatctcaa aaacattctt cccatgaagg ccgtatacga tcgcaattat
      481 tgccgccgcc tcgaggccac tcacgttaaa tcagaaacaa acaagatggc tcaagcggaa
      541 gccctcatca aagacatcgc cgaattcaaa accaaaaata actgtgatcg tctgaccatg
      601 atctggtgcg cctccaccga ggccttcaac acggtcaccg aggcacataa atcagtccat
      661 gccctcgaag aaggcatgag aaacaacgac cccacgatct cacccagtat gatctacgcc
      721 tatgccgcgc tcaagtcgcg aatcggctac gccaatggag ctcccaacct gacgacagaa
      781 atccccgcct tgcaggaact cgcaaaagaa atgcgtgtgc ctctttctgg taaagacttc
      841 aagacaggtc aaaccctcat gaaaaccatt gtggcccctg gccttgcggc ccgctacctt
      901 ggcatgaccg gctggttctc aacaaacatt ttgggcaatc gcgatggtga ggtcctggat
      961 gatcctcaaa acttcaaaac gaaagaggaa tccaagttat cagtgctcga ttcgatcctc
     1021 aatcccgaac tctaccccga tttgtacggc gatctctacc ataaggttcg cattgaatac
     1081 taccctcccc gcagagacaa caaagaaggc tgggacaaca tcgatctcgt cggatggttg
     1141 ggcatgccca tgcaactcaa gatcaacttc ctgtgccgtg attccatatt ggccgccccc
     1201 atcgtgctcg acctggtcct gttcatggat ctggcccaac gcgcggaact caagggcatc
     1261 caggaatggt tgtcgttttt ctacaaatcg cctatggtga aggaagggct cacgccaatt
     1321 cataacctgt tctcacagga gatgaaattg cagaacaccc ttcgttacct gatcggcgaa
     1381 gaacagatca cctttttggg actggactat tacgagtaag ctgtctgccg acagctgaca
     1441 gccaacagct aaccgccaaa cagttctggc cgttggccgt cgactgtcag ccgtgggctc
     1501 atcgtttgat acatgttttt gaacagaaca caaagacagt ccttttcttc gattggcagt
     1561 ctcctcaaga aaaagttggc tgggccagag catgaacctc tacaaaaaac cgtggctctg
     1621 caccagcagt ggttgaagat cgttggtccc attatttcga gacactcccg aatcctgtac
     1681 atcaaagatg gttgtttgtt cgtcagcgta gcgcaccccg catggcataa cgagctttct
     1741 ctcatgaaaa accagatcct caaacagata caagaacaac tccccgacac caaggtcaca
     1801 agcattaaat ttaaaacgga cgcggagagt caatatgaaa aaggaacaaa aaggaccttt
     1861 taacaaaatt ctagaggtca atcttaccaa acagattttc actgtttgca ctgtcaccaa
     1921 aaaagatcgc gtcatgtatc tgggcgcgaa aggtttggcc ctcaaactcc tctttgatcg
     1981 cgtgaaaccc ggagtcgatc ctttggggcc agacaattac ctcgcttttt tcatgggcgt
     2041 ggtgatgggc accggagcac cgtgttcagg gcgtttctcg tctgtgacca aatcacctct
     2101 cacgggaatt ttcacacact catcctgcgg cggacccttt ggcatgcaac tcaagacagc
     2161 gggttgggac ggcgtcctca tcacaggcgc ggctaaaaaa cccacttatc tcgagattac
     2221 cgaaagcggc gttgccttca aagacgcctc atcactctgg ggtctggata cagtggaaac
     2281 ccaaaaacgt tttggcgaca aagggtctgc gctggtcatc ggtccggcag gagaaaacgg
     2341 ggttttgttc gccaacatcg cttcgggcca ccgttttctg ggtcgcggag gcgcgggaac
     2401 tgtgatgggc gccaaaaatc tcaaggccat tctggccatc ggcgacgttt ataaaatatt
     2461 gcccatcaat caaaaagatt tcgatcaggt caaaaagcag gctacccatt acatcaactc
     2521 caacgatgtc acctccgaca aataccgcaa ctatggtact tctgccaatg tccgtctttg
     2581 caataaggca ggcatcctgc ccgtcaacaa ttttaccaaa ggacagcacg aggacgccga
     2641 caacatctct ggagaaactg tcaaagaaag attcaatacc aagcacgcca cgtgtcgccc
     2701 ctgcaccatt ttgtgcggga aaaaaggcac ttttgaggga caggaactca ttgtttccga
     2761 atacgaaaca gtggggctgt tgggttcgaa tctggggatt tttgatccaa ttaaaatcgc
     2821 gcactggaac aaactctgcg gggatatggg catggatacc atc
//
LOCUS       DATDUN010000269         4532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_263186, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000269 DATDUN010000000
VERSION     DATDUN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263186"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4267)
                     /locus_tag="VJC18_03690"
     CDS             complement(<1..4267)
                     /locus_tag="VJC18_03690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HLD44512.1"
                     /translation="MLTPGPALANAAVVRYAATTPPFSKNTAGVSLALSPADSCFIAL
                     HESLDPLGITVCDVSTFSATVKTGLTEFMGRACLLLDKLLNDDEHKQTFHFVLVEDLR
                     VNACHSSQRDDSIVIGVTLGLIREIGSDEELAFVLAHELEHHHSQIEKDRREKMEARQ
                     TPRNDDLLDTIAWAFKRRSVEHECDVRAVKRLINKQISPQGGLRILSKVRARRLANNP
                     TELPAEGHPLSMTRINAVGAILADSVRHQGQAQNYRLEQQTQTTEVLGNVHELLFKTD
                     CLQNECFEKARAFINPDRVVDEAYQAIADNHKPETLSVEAAEHYEPASCFPEIFNYYW
                     SRASRLLMPELPAEQRLDLQLQLVVNLDRAFKRAKQEILGDDFTVSEYWQYEMLLLLE
                     KRTLDLDIPSIQLCLVQRAYCVEAIKKHGTIPDDTLSLETKQQNLARIERQLEIAKHV
                     FEPGKKLEQYMAKGYTKEQLDVLLLDRRGRRFLDVELARHGLRIREVHDIIVGNKQQL
                     EAIKRKNGFQVLLAATDIMHFIGLLNHYYSGFSRTGQQATNPSGNFIKVLHKAKALVD
                     EALARCENGNLDKQTFEQIKTLISSISFHGDYSAPHTPEVFAHRQGFLDKAISILFSQ
                     KQLFSSTEELFNFECQIVSPSLAVLGPRGAVIKEEVQRLLVETAMTPIKQHIANGHIH
                     EAMLALSDLTKQHKTLRTTILDSLDPVARTNINHHMLTLLKEWANAIGLTSDQLQTFV
                     WITVPQFMPADVNSEQLAKAKADFHALCQNKNFTASLQGRCFHFWAVMHVFCNSGHAK
                     AHLSEAYALAVMAQELWYSIDSLYYNVNFEQSLHDYLFKRLTVERPWDLKLAKILIQQ
                     VEQPTVSKSMPFLQACFRWYIDRFHGVTEATYQFWLAFDEAGISPLQDQLVLIECLIN
                     SFYTHAISPDADPSKHHLISLEFFEAYRAGVVRFAEQRQAEGGAKTTYYKTNYDEPHD
                     QIPVKGGKALDMNLRISWQPLNDFWSEPDNYETLPIALVATAFEHWITEYGYHGINYL
                     GHSGSQTSYHVLMFLFEHHRDHPDVRRVLLRAEMVKAAFFQSAKSRLVKWQLEQWPGF
                     ADFLPNLRAGKGRLPCKEELRPIISDIHEFLRIRFPEPCSASYEVVSFVERHLLTSKT
                     ETELLNQLRVTEQNWTSNPALYAPDAADFIAQALRTDQERMGTLRYLLGIDDDPPQLQ
                     CFSDRFAINTYSPHMVGELKKRVAHGNEFFKILCVQALLADRVNAIESQLYLECKTLI
                     MGEATQHEALSVLFDEYMRVLPCSERKILLALAIAAYDPSTGDHVSFKRLLQAAGPLG
                     AKMSQALVTSGAVTGSLRAELLTSFDDALPPTRKDIFDDLAKIFPDDNIVQSVGRLLG
                     SGSLNYTVEALLRSPVEGVAQRVAVRIQKANVRGQIDNEYLVWS"
     gene            complement(4279..4500)
                     /locus_tag="VJC18_03695"
     CDS             complement(4279..4500)
                     /locus_tag="VJC18_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44513.1"
                     /translation="MSCQTDHYRLSLKALSFPQVLLLVLSAEQPQAFSSLLQRPKDRQ
                     NQSQRPVQSPIWKAQNHISLLRHPLSLET"
BASE COUNT         1132 a         1016 c         1154 g         1230 t
ORIGIN      
        1 ttgaccaaac gagatactcg ttatcaattt gacctctgac gttggccttt tgaattctca
       61 cagccacgcg ctgcgcaaca ccctcaactg gtgatctgag caaggcttca acagtgtaat
      121 tcagggagcc agaccccaga agacggccca ccgactggac gatattatca tcaggaaaaa
      181 tctttgccag atcatcaaaa atatctttac gagttggggg cagggcatca tcaaatgagg
      241 tcaagagttc ggcacgcaag gaacctgtga ccgcgccact ggtgacaagc gcctgagaca
      301 tcttggctcc caagggacca gcggcctgca aaaggcgttt gaacgataca tgatcacctg
      361 ttgagggatc gtaggccgct atggccagag ccagaaggat ttttcgctcc gaacagggta
      421 gtactcgcat gtattcatca aacaggacac tgagcgcttc gtgctgtgtg gcctctccca
      481 tgatgagtgt cttgcattcc aaatagagct gagactcaat cgcgttgacc ctgtctgcaa
      541 gaagggcttg aacgcaaaga attttaaaaa actcatttcc gtgagccact ctttttttta
      601 attcgccaac catatgtggt gagtatgtgt ttattgcaaa acggtcagag aagcattgta
      661 gttgtggggg atcatcatca atccccagaa gataacggag cgtgcccata cgttcctggt
      721 ctgtacgcaa ggcctgtgca ataaaatcag cggcatcagg ggcgtacaag gcgggattgc
      781 tggtccagtt ctgctcggtg acgcgcagtt gatttaacaa ttccgtttcg gttttgctgg
      841 tcaggaggtg tcgttccaca aaactgacca cctcatagct ggcgctacag ggctccggga
      901 agcgaatcct caaaaattca tggatgtctg aaatgatcgg tcgtaattcc tccttgcatg
      961 gcaatcgccc ttttccggca cgtaaattgg ggagaaaatc agcaaagccg ggccactgct
     1021 ccaactgcca cttaacgagc ctgctcttgg ctgattgaaa gaaggcggct ttcaccattt
     1081 cggccctcaa tagaacgcgt ctgacgtcgg ggtggtctcg gtgatgctca aagagaaaca
     1141 taagaacgtg gtagctggtt tgagagccac tatgacccag atagtttatt ccgtgataac
     1201 cgtattctgt tatccagtgc tcaaaggctg tggccaccaa tgctatgggg agtgtttcgt
     1261 agttgtcggg ttcactccag aaatcattca gaggttgcca gcttatgcga agattcatgt
     1321 cgagggcctt gcccccctta acaggtattt gatcatgggg ttcatcatag ttggttttgt
     1381 aatacgtggt tttggccccc ccctctgcct gcctttgttc ggcaaatcgg acaaccccag
     1441 cccgataggc ttcaaaaaat tccagactga ttaaatgatg tttgctgggg tcagcatcgg
     1501 gcgagatggc gtgcgtataa aaactgttaa tcagacattc gatgagaacc aattgatctt
     1561 gcaagggtga aatgccagct tcgtcgaagg ccagccagaa ttgataagtg gcctcagtga
     1621 caccgtggaa acggtcaatg taccagcgaa aacaggcctg aagaaaaggc atgctttttg
     1681 agactgtggg ttgctctacc tgctgaataa gaatctttgc cagtttgagg tcccagggtc
     1741 tctccacagt gagtctttta aacagataat catgaaggga ctgctcgaaa ttgacattat
     1801 aatataaact gtcgatcgaa taccagagtt cctgtgccat gacagccaaa gcataggcct
     1861 cggacaagtg tgccttggcg tggcccgaat tgcaaaagac atgcatgact gcccagaaat
     1921 gaaaacaacg accctgaaga gaggctgtga aattcttgtt ttggcacagg gcatggaaat
     1981 ctgcttttgc ttttgcgagt tgttcggagt taacatcagc tggcatgaat tgggggacag
     2041 tgatccagac aaatgtttgt aactggtcag aggttaaccc gatggcattg gcccattctt
     2101 tcagcagagt gagcatgtgg tgattgatgt tggtgcgtgc tacgggatcc agtgaatcga
     2161 gaattgttgt tcttaatgtt ttatgttgtt tcgtcaaatc gctcagcgcc agcatggctt
     2221 cgtggatatg accgttggca atgtgttgct tgatgggtgt catggcggtc tcgaccagaa
     2281 gcctttgcac ctcttctttg atgacagcgc ctctggggcc caaaacagca agtgacggcg
     2341 aaacaatttg acattcgaag ttgaaaagtt cctcagtaga actgaataat tgcttttgtg
     2401 agaacaaaat ggaaatggct ttatccagaa agccttgcct gtgagcaaac acctcagggg
     2461 tatgtggggc agaatagtcg ccatgaaaac tgatggagga gatcagggtt ttgatctgct
     2521 caaaagtttg tttatcgagg ttaccatttt cacacctggc cagggcttca tcaacaaggg
     2581 cttttgcctt atgcaggact ttaataaaat tccctgaggg gttcgtggct tgttgtcctg
     2641 ttcgggagaa tcctgaataa tagtgattga gcaggccaat aaaatgcatg atatcggttg
     2701 cggccaacag gacctggaaa ccattttttc tttttatggc ttccaactgt tgtttgtttc
     2761 ctacgatgat atcatgaact tctcttattc tgagaccatg acgtgctaat tctacgtcaa
     2821 gaaaccggcg ccctcgtctg tccaacaaaa gaacatccaa ctgttctttt gtgtatcctt
     2881 tggccatgta ttgctcgagc ttctttcccg gttcaaagac atgttttgct atttcaagtt
     2941 gtcgttcgat acgagccaga ttctgttgct tggtctcgag tgacagcgtg tcatcgggta
     3001 tggttccgtg cttcttgatt gcctcaacgc aatacgcccg ttgtaccaga cataactgta
     3061 tgctggggat atccagatca agggtccttt tttccagaag gagcaacatc tcatactgcc
     3121 aatattcgga aacagtaaaa tcatccccca gtatttcttg tttggcgcgt ttgaacgccc
     3181 tgtccaggtt cacgacaagt tgtaattgaa ggtcaagtct ttgttccgct ggaagctcag
     3241 gcattagtag tcgggaagca cggctccagt agtagttaaa tatttcagga aaacaagaag
     3301 ctggttcata gtgttctgcg gcttccacag acagtgtctc tggtttgtga ttgtcagcaa
     3361 tggcctgata agcctcatca acaacgcggt caggatttat gaaggccctg gctttttcga
     3421 aacattcgtt ttggagacag tctgtcttaa aaagaagctc atgaacattt cccaaaactt
     3481 ctgtcgtttg tgtttgctgt tccaatcgat aattttgtgc ctgtccctgg tgtcgcaccg
     3541 aatctgccag gatggcaccg acagcattaa tgcgtgtcat gctcagagga tggccctctg
     3601 ctggtagttc agtgggattg tttgccagac ggcgggcgcg aacttttgaa agtattctca
     3661 gaccaccctg gggactgatc tgcttgttga tcaatctctt gactgcgcgg acgtcgcatt
     3721 cgtgttctac agaacggcgt ttgaatgccc acgctatcgt atcgagtaga tcatcgtttc
     3781 ttggggtttg tctggcttcc attttttccc gtctgtcttt ttcaatttgt gaatgatggt
     3841 gctccaattc gtgggccaaa acaaaagcca gttcttcatc ggagccaatt tctctgatga
     3901 ggccaagagt cacaccaata acaatagaat catcacgttg actgctgtga caggcattta
     3961 cccgcaaatc ttccaccaaa acaaaatgaa atgtctgttt atgttcatca tcattcaaga
     4021 gcttgtccaa tagtaggcac gcgcggccca taaattctgt cagacctgtt ttcactgttg
     4081 cggaaaaagt actcacatcg cacacggtga tgcctaaagg atccagggac tcatgtaatg
     4141 ctataaaaca agaatcagcc ggtgacagcg cgagagaaac gcctgctgtg ttttttgaaa
     4201 agggtggtgt ggttgccgca taacgtacca cagcggcgtt ggctaaagca gggccaggcg
     4261 ttaacatgtc aatggcggtc aggtttccag agagagtggg tgtcttaaca gcgagatgtg
     4321 gttctgtgct ttccagattg gcgattgaac tggacgttgt gactggttct ggcggtcttt
     4381 cggtctctgc aacagagagc tgaaggcctg aggttgctct gctgagagga ccagcaggag
     4441 cacttgtggg aagcttaagg ctttgaggga caagcggtag tggtcggttt ggcaggacac
     4501 aagtcattat tctccaaaag gcagaaatgg ta
//
LOCUS       DATDUN010000270         2646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_179088, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000270 DATDUN010000000
VERSION     DATDUN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179088"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VJC18_03700"
     CDS             <1..1266
                     /locus_tag="VJC18_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLD44514.1"
                     /translation="YRLNLSDCIEMAVRNNEEIKAQEYDVQAYIARKIEATKRYVPVV
                     TIKYRMAPVPRDLNNPAVSFLSGDISVFNSIKLEAGIPITTFGRIEVNKHLAEIGIDA
                     AQLKTKQKTDEVILDIHKLYHGILLARELKVLANKGLDAIHEKIAELEKEENIDQLQI
                     LKLKAVYYEVEKRLDEAHKKETIALAMLKVRTGIGDDVDFDIKDKALTTDYLAIEAYE
                     NLLQTSKAKRPEFQLLQHQVNAKNLQVKLEKKAYFPMLKFGGYFEYGNSPHIIGDEDD
                     NTFNNPFNYTRAAAGLELSNELDFRKIQANVEKAKAEHLKAIAEKRSNDRLLEVDLKN
                     AYLDFIQKQKFLSRAEKEQRAAREIVFLTKSNLDIGLGEKKEYLDALQSYLVIQAAVL
                     ENIYNYNMAAATLKQKLGTIGQEARTKAL"
     gene            1277..2062
                     /locus_tag="VJC18_03705"
     CDS             1277..2062
                     /locus_tag="VJC18_03705"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLD44515.1"
                     /translation="MTSHKPLFIPIILFLIFVGGCSSKKNPEAPSSSPSDQVAVTNQI
                     QYPPEEEYNPKALDPKEIGTPMQAIKGLDQMIETYHLGKELTAEQTEHNSSLKQKIIR
                     GTFDIKELCRLSLGKHWDAISEAQQKHFVGMMTTLLETKAIFSKEQLKGTNKYYSISY
                     KNESFDDETKEKATVFTDMHVPKEKITLNITYKLLLTPYGWKIYDVVVDDASLLTNYK
                     FQFNQIISTKGFDELFARMEKQLNKIKGVPPDNNQTTGGAHQK"
     gene            2059..>2646
                     /locus_tag="VJC18_03710"
     CDS             2059..>2646
                     /locus_tag="VJC18_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLD44516.1"
                     /translation="MTPAQTPFQPFKRSILFLIDGARADVMTHEIMQGHLPHLQKFFV
                     QDGCQKTMLTCFPSTTGPAHLPYLTGAFPGTCNIPGIRWFDKASYAKKGWGFRSIRSY
                     CGPENRLLDRDLAANITTAWHVFNHPYNILGGLTKGLIKEHNLTRQQRELYYYYAHLT
                     DRWSLIDHLAAQKLESIVTKREFDFIFTVFPGVDEY"
BASE COUNT          830 a          698 c          550 g          568 t
ORIGIN      
        1 taccgtctga atttgagcga ttgcattgaa atggccgtca gaaacaacga ggaaatcaag
       61 gcgcaggaat acgatgtgca agcctacatc gcccgcaaga tcgaagcaac caagcggtat
      121 gtccctgttg tcaccatcaa ataccgcatg gccccagtcc caagagactt gaacaatccc
      181 gctgtatcgt tcctctcggg agatatctcc gttttcaatt ccatcaagct tgaggcaggg
      241 attcccatca ccacattcgg ccgcatcgaa gtcaacaaac acctggccga aatcggcatc
      301 gatgccgccc agctcaagac aaaacaaaaa accgatgagg tgatcctcga catccacaag
      361 ttgtatcacg gcattctgct ggccagagaa ctcaaagtgc tggccaacaa aggtctggat
      421 gccattcatg aaaaaattgc cgaactggaa aaagaagaga atatcgacca gcttcaaata
      481 ttaaagctca aagcggtgta ctatgaggtc gaaaaacgcc ttgatgaagc acataaaaaa
      541 gaaaccatcg ccctcgccat gctcaaagtc cgcaccggca tcggggatga tgtggatttc
      601 gacatcaagg acaaggccct cacaacagac tacctggcca tcgaagccta tgaaaacctg
      661 ttacagacgt ccaaggcaaa acgtcccgaa tttcagttat tacaacatca ggtgaacgcc
      721 aaaaacctgc aggtcaagct tgaaaaaaag gcctattttc ccatgctcaa atttggtggg
      781 tattttgaat acggcaacag ccctcacata atcggtgacg aggatgacaa tacgttcaat
      841 aaccctttca attacacacg cgcggccgcg gggcttgaat tgtccaacga actcgatttt
      901 cgaaaaatcc aggccaatgt cgaaaaagcc aaggctgagc acctcaaggc catcgccgaa
      961 aaacggtcaa atgatcgttt gctcgaggtc gatttgaaaa atgcctattt ggattttata
     1021 cagaagcaga aatttttatc ccgtgccgaa aaggaacagc gcgccgccag ggagatcgtt
     1081 tttttgacca aaagcaattt ggacatcggt ctgggagaaa aaaaagaata tctggatgcc
     1141 ctgcaatcct atttggtgat tcaagccgct gttttagaaa atatttacaa ctataacatg
     1201 gctgcggcta cgctcaaaca aaaactcgga acgatcggcc aagaggcaag aactaaagct
     1261 ctttaaggag gatcttttga caagtcacaa acccctattc atccccatca tactcttcct
     1321 cattttcgta ggtggttgct catccaaaaa aaatccggag gcgccatcat caagtccatc
     1381 agaccaggtc gctgtaacca atcagataca atacccaccg gaggaagagt acaaccccaa
     1441 ggctcttgac ccaaaagaaa ttggcacgcc catgcaagcc atcaaaggtc tcgatcaaat
     1501 gatcgaaacc tatcacctcg ggaaagagct cacggctgag cagacagaac acaacagttc
     1561 actcaaacag aaaatcatac ggggcacctt cgacatcaaa gagctgtgcc gcctgtcgct
     1621 tgggaagcac tgggacgcca tatccgaagc ccaacaaaaa cattttgtgg gcatgatgac
     1681 gaccctgctt gaaaccaaag ccattttttc caaggaacag ctcaaaggca caaacaaata
     1741 ttacagcatt tcctataaaa atgaatcttt cgatgacgaa acaaaagaga aagccacggt
     1801 cttcacagac atgcatgtgc ccaaggaaaa aatcacattg aacatcacgt acaagctcct
     1861 gctcacaccc tatggctgga agatttacga tgtcgttgtc gacgatgcct ccctactcac
     1921 caattacaaa tttcaattca accagatcat ttccacaaaa ggttttgatg agctctttgc
     1981 ccgtatggaa aagcagttaa acaaaatcaa gggggttccc ccggataaca accaaacgac
     2041 aggaggcgct caccaaaaat gactcccgca cagacaccat ttcaaccttt caaaagatca
     2101 atactatttt taatcgatgg cgcccgagca gatgtcatga cgcatgagat catgcagggc
     2161 cacttgcccc atctgcagaa attttttgtt caggacggat gtcaaaaaac aatgctgacc
     2221 tgttttccct ccacaaccgg gccagcgcac cttccttacc tcaccggagc ctttcccggc
     2281 acatgcaaca tccctggcat tcgctggttc gacaaagcgt cctatgccaa aaaaggctgg
     2341 gggttcaggt caatccgtag ttactgtgga ccggagaacc gactgctcga ccgcgatctt
     2401 gctgccaaca tcacgaccgc ctggcatgtt ttcaatcacc cttacaatat tttaggaggc
     2461 ctcacaaaag gtctcatcaa agaacacaac ctgacacgac agcaacgcga actctactat
     2521 tactacgcgc acctcacaga cagatggtcc ctcatcgatc atctggccgc tcagaagctt
     2581 gagagcattg tgaccaagag agaatttgat ttcattttta cggtatttcc cggagttgat
     2641 gagtac
//
LOCUS       DATDUN010000271         3574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_347805, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000271 DATDUN010000000
VERSION     DATDUN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347805"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="VJC18_03715"
     CDS             <1..1621
                     /locus_tag="VJC18_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HLD44517.1"
                     /translation="VSGEGRSFSTTQTYLPHTNILKTTFDDGTNAFEVIDFMPRYRDG
                     ESYAQPIELHRFIRPLRGAPVIRINFQPRLNYAQGPTKISIREQASVITAANGFESVF
                     LYSSLPVKEIGEGLPVLLKEEHFLVLSYHEKFTLPTPDVVRALLFKTTEYWEAWSAKC
                     HLPKLYAEQVLRSALTLKLLTYSSSGALLAAATTSLPEIVGQDRNWDYRYCWLRDSSM
                     IVEALKSLGHFEEAQAFIKFLLNILESKQTKIQILYGIGGRMQLEEIELAHLSGYQGS
                     KPVRVGNNAWKQKQNDIFGEIIQAIYLYYFHYKIDPITPEVWSLIKFLANTISKEWNT
                     PDAGIWEFRHEGQHFTFSKVLSWVALDRAATVANLIGKTYAAERWSAAARLVKEDIEN
                     NGWSNNAGAYTQVYGSKALDASLLLMNRYGYLAADDPRWIQTVLACRKGLMNHGWVMR
                     YTNMDDFGKPKNAFFVACLWLAQALISIGQIDEGKILFENVLAKSNHLGLLSEDIDVK
                     TGEQTGNFPQAYSHIEVIRTAALLSDILKEK"
     gene            1635..2405
                     /gene="otsB"
                     /locus_tag="VJC18_03720"
     CDS             1635..2405
                     /gene="otsB"
                     /locus_tag="VJC18_03720"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HLD44518.1"
                     /translation="MNLFSIKNLSKIGGTKGLFLFLDFDGTLSPLVSDPTQAVLLPGL
                     KRTLTKLATYSTVRIMIISGRSLADLRKRVGIPGLIYAGNHGCEIFFKKRYLLRKGEK
                     YKPIIRQATALLKKQLAQIPGVIIEPKGFSVAVHFRKTPKEYLSEIKMILCSIAQQES
                     INKVMLTAGKKIFELRPHQDWNKGKAVLWAWKKLAPEYLPVYLGDDVTDEDAFRALKK
                     SGVTIRVGKKKNSAAQYYVLKPKNVAEFFKRLNQRKGP"
     gene            2408..2989
                     /locus_tag="VJC18_03725"
     CDS             2408..2989
                     /locus_tag="VJC18_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44519.1"
                     /translation="MSLYPALPFALLLLCTALPPLMAHHFWEKARNKALVALGLSRNG
                     VRWGKNEEMGDPMHRTNTLLSPEPLLVRAAQHLITNAGDTAPELSDPWYKGLLHFLAN
                     LAGARTLTYRDEHLLPDNLEGVSFQGVRVDKKIAEAIVRKNGSLNGAILVGGFFGTML
                     NKEQLCKTFRFNTKGEKIRVTTDWLEEQRAMTT"
     gene            complement(2995..>3574)
                     /locus_tag="VJC18_03730"
     CDS             complement(2995..>3574)
                     /locus_tag="VJC18_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD44520.1"
                     /translation="VSRATLLKLSRTYWWVVLIGAVLTLARFSEAFLVLRALQGGLSL
                     GLIPLVLLVMNLVYALGAYPFGKLADKIQHRILLIVGILPLIVADLVLAHSAQPIWLC
                     VGLVFWGTHLAATQGLLAAMVADTAPEDLRGTAFGFFNLISGLAMLIASTLAGVLWDQ
                     LGAPATFYAGAVFSGVALLFLFVRGDNSIGIE"
BASE COUNT         1031 a          884 c          804 g          855 t
ORIGIN      
        1 tgtcagcgga gagggccgtt ccttttcaac aacgcagacg tatctgcccc acacaaatat
       61 tttaaaaaca acctttgatg atggaacaaa cgcctttgag gtcattgatt tcatgcccag
      121 atatcgtgat ggagagtcct atgcgcaacc catcgaactc caccgtttca tacggcccct
      181 gcgcggcgct cctgtgattc gcatcaattt tcaaccccga ttaaattatg cccagggccc
      241 cacaaaaatt tccatcagag aacaggcctc agtcattacc gcagccaatg gttttgaaag
      301 tgtttttttg tattcgtcac ttcctgttaa ggaaatcgga gaaggcttgc ccgttttatt
      361 gaaagaagag cattttctgg ttttgtcgta ccatgaaaaa ttcacacttc ccacacctga
      421 tgttgtgaga gctctgcttt tcaaaacaac ggaatattgg gaagcctggt cagccaagtg
      481 ccacctgcca aaactctatg ccgagcaggt cttgagatca gccctcacgt taaaactgct
      541 gacctattca agttccggcg ccctgttggc cgcggccaca acatctttgc cagaaatcgt
      601 gggccaggac cgcaattggg attatcgcta ctgttggctc cgcgattcct ccatgatcgt
      661 tgaggccctt aaaagtctgg gtcactttga agaggcacaa gccttcatca aatttctttt
      721 gaatatcctg gaaagcaaac agacgaagat tcaaatcctt tatggcattg gcggccgaat
      781 gcagcttgaa gaaatcgaac tcgctcacct ttcaggttac caggggtcaa aacccgtgcg
      841 cgtgggcaac aatgcttgga agcaaaaaca aaacgacatc tttggtgaaa tcattcaggc
      901 catttatttg tattactttc attacaagat tgaccccata acacctgagg tctggtcgct
      961 catcaagttt ctggccaaca ccatttcaaa ggaatggaac acacccgatg ccggcatttg
     1021 ggaatttcgg cacgagggtc agcacttcac cttttcaaag gttctttcgt gggtggcgct
     1081 ggatcgagca gccaccgtcg ccaacctgat tggaaaaact tatgcggccg aaagatggtc
     1141 agccgctgcc cggttggtca aagaagacat tgaaaacaat gggtggtcaa acaacgcagg
     1201 tgcctacacg caggtttatg gatcaaaggc gctcgacgcg agccttttgc tcatgaatcg
     1261 ttacgggtat ttggctgccg acgaccccag atggattcaa actgttttgg cttgccgaaa
     1321 gggtttgatg aaccacggtt gggtcatgcg ttacacgaac atggatgatt ttggaaaacc
     1381 caagaacgcc ttttttgtgg cctgcctttg gttggcgcaa gccctgatca gtattggcca
     1441 aatcgatgaa gggaaaattc tttttgaaaa tgttctggcc aaatcaaatc atctgggcct
     1501 tttatccgaa gatattgatg tgaagacagg cgaacaaacc gggaattttc cacaggcgta
     1561 ttcgcatatt gaggtcattc gaacggccgc cttgttgagt gatattctga aagaaaaata
     1621 aaaataagaa agccatgaac ttattttcta ttaaaaactt gtcaaaaatt ggaggtacaa
     1681 aaggtctttt tctttttctc gattttgacg gtacgctatc ccctcttgtt tctgatccta
     1741 cgcaagccgt tttgctcccc ggactcaaac gaacactaac aaaactggca acatattcca
     1801 ccgtgcggat tatgatcatc tctggacgtt cactggcaga ccttcgcaaa cgtgttggaa
     1861 ttccaggtct catttatgcg ggcaaccatg gatgtgaaat cttttttaag aagcgttacc
     1921 ttttgcgcaa aggagaaaaa tacaagccga tcattcgcca ggccacagcc cttcttaaaa
     1981 aacagctagc ccaaataccc ggtgtcatca ttgagcccaa ggggttttcg gtagcggtgc
     2041 actttcgtaa aacccccaag gaatatcttt cggaaatcaa aatgatattg tgttcaatcg
     2101 ctcaacaaga aagcatcaat aaagtgatgc ttacagcagg taaaaaaatc tttgagcttc
     2161 gacctcatca ggactggaac aagggaaagg ctgtattgtg ggcctggaaa aaactcgccc
     2221 cggaatatct tcctgtttat ctgggcgatg atgtgacaga cgaagatgct tttcgtgctc
     2281 taaaaaaatc gggcgtcacc attcgggtgg ggaaaaagaa aaacagcgcg gctcaatatt
     2341 atgttctcaa gccaaaaaat gtggccgagt tttttaagag gttaaatcag agaaagggcc
     2401 catgagcatg agtctttatc cagcacttcc ttttgccctt ttgttgctct gcaccgccct
     2461 cccgcccctg atggcgcatc acttttggga gaaggccaga aacaaggcgt tggtggctct
     2521 tggactttct agaaatgggg tcagatgggg caagaatgaa gaaatgggtg accccatgca
     2581 tagaacaaac accctattat cacctgaacc tcttctggtg cgagccgcac aacatttgat
     2641 caccaacgca ggcgataccg caccagaact atcagaccct tggtataaag ggcttctgca
     2701 ttttttggcc aatctcgccg gagccagaac attgacctat cgcgatgaac acctccttcc
     2761 agataatctt gaaggtgtgt cctttcaagg tgtgcgtgta gataaaaaaa tcgcagaggc
     2821 catcgtcaga aaaaatggtt cattgaatgg cgccatactt gttggtggtt tttttggcac
     2881 aatgctcaac aaagaacaac tgtgtaaaac tttccgcttc aacacaaaag gtgaaaagat
     2941 acgagtaacc actgattggc tggaagaaca gcgtgccatg acaacataaa aacatcattc
     3001 gataccaatg ctgttatccc cacgtacaaa taaaaacaac aacgcaacac cggagaaaac
     3061 cgctccggca taaaatgtgg cgggcgcacc cagttgatcc cacaacacac cagccaaggt
     3121 gctggcaatc agcatggcca acccgctgat cagattaaaa aaaccaaaag cagttcctct
     3181 caaatcctct ggcgcggtat ccgcgaccat tgcggccaac aagccctgcg tagccgccag
     3241 atgagtgccc cagaacacca aacccacaca aagccagatg ggttgcgcgc tgtgtgccaa
     3301 aaccaaatca gcgacaatca acggcaaaat gccgacaatc aacaggatcc ggtgttgtat
     3361 tttatccgcc aactttccaa acggataagc accaagcgcg taaaccagat tcatgaccag
     3421 caacacgagg gggatcaagc ccagagacaa gcctccctgt agggctcgca aaaccagaaa
     3481 ggcctcagaa aatcgagcca aagtcagcac agctcctatc agcacaaccc accaataagt
     3541 ccgggatagt ttaagcagag tggctcgcga aaca
//
LOCUS       DATDUN010000272         5636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_263346, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000272 DATDUN010000000
VERSION     DATDUN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263346"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VJC18_03735"
     CDS             complement(<1..940)
                     /locus_tag="VJC18_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44521.1"
                     /translation="MSNEIHYIESDYCYDDYDDLGEQNTCEAEESDFEACASEAYAEK
                     SCLLATRAFGVDDDVYFKEDNVARRTLMSAEHVSNASFAQDQFLAITKPPLMKILGNG
                     PAQEVANVLLRGYRNYVSADRDTTNNETMWGSQEPKGFVYEVLDGAKALAREVTQKTG
                     LDDFSFEKAEREVRDSIESVYHEMLREDEEKAELVANEEDVYEEQEWSEDESDYSEDD
                     VCFSSEDYSASCWYEECEQELKTDNAEETSDEQEELETNENQQEFLMPITELVAAVPN
                     EPQPLIIYSTLRQPTGQEGAGTTSTATRQPPSPQDQP"
     gene            complement(1171..2103)
                     /gene="truB"
                     /locus_tag="VJC18_03740"
     CDS             complement(1171..2103)
                     /gene="truB"
                     /locus_tag="VJC18_03740"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HLD44522.1"
                     /translation="MSEPSYFLFLHKPKGMTSQACLTIFKKKHKIKKIGHHGTLDPFA
                     EGLLLVGVNEATKYFCYIDDEKKTYQATIVFGKQTDSLDVTGREVASGEVPYLSREQI
                     VLAMATFLGESQQTPPMFSAIKVNGKKLYELARQGVAIPRQARRIFVFELDVLFWESP
                     VLKIVTTVSRGTYIRVLASDLAKVLGTMGYLSELSRTGLCGAGLEQAMSLDQEEVLLS
                     QQIAIADMLSQYRSLYLNQEGEKRLFQGKLIGRECVISELPPSAMSATQGLTNNVCKV
                     FHDKKFLGLATLERDVIRAKRLINHKKNDATSVS"
     gene            complement(2100..3083)
                     /locus_tag="VJC18_03745"
     CDS             complement(2100..3083)
                     /locus_tag="VJC18_03745"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HLD44523.1"
                     /translation="MTSKTKKISQKKIISSLLGASQILVTTHENIDGDGLGAMIALGQ
                     GLVQLGKKTCLYSKDGVPRIYQFLPGQKSVCQKLDLHKKFDLTLVLDVAERKRVSDVF
                     LAHRFLGTVISIDHHVTGSPDTDYIFSRPQQASCGELIYSLLAKLPVQITKEMATNLY
                     TAIATDTGSFKYSNTKPQTLTTAARLLALGVDVWQVSQNCFEMYSLPRMALLKRLMPK
                     IEVHPCGKIAWVVLTQHDFKVTCAKSEDAEGFINYPRFIEGVEVAVLFKQKSSRVYKV
                     SMRSKEFVDVAAIAAKFGGGGHVRASGCTIEGSLTEIKKQILTEILFSLRK"
     gene            complement(3080..3436)
                     /gene="rbfA"
                     /locus_tag="VJC18_03750"
     CDS             complement(3080..3436)
                     /gene="rbfA"
                     /locus_tag="VJC18_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HLD44524.1"
                     /translation="MKDKRQNRISQAYQKELTSILLYEVRDPRLDGLCVTDVVITPDL
                     RLAKVYYDIPGGRERVVAVEKGFQKAKGYIKKELARRVNIKYMPDLVFYFDETAALQE
                     RVDQLFAEVKRSPDQQ"
     gene            complement(3450..>5636)
                     /gene="infB"
                     /locus_tag="VJC18_03755"
     CDS             complement(3450..>5636)
                     /gene="infB"
                     /locus_tag="VJC18_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HLD44525.1"
                     /translation="EAELKASKKPKPKEEERPRILSFKDRIRGHIDLKKTKPEDVAKE
                     QKLRVAGEPGLAGHVDAGNETAEEKAEKQKKLIRSRKGVKAIGGDLDIEGYGRTQTLA
                     QLSRLVPAERIFRPSDMGRKKKKVVSRKNLKQTVLTEKKTSKRYVEIDQKITVGNFAQ
                     GLGVKAGEIIKRLMDLGVMATINQEIDKETATLIAAEYQYEVRDISFKEDKLLMAKQS
                     VAENENGNPSRPPVVTIMGHVDHGKTSLLDKIRSTNVASGEAGGITQHIGAYTVVLPQ
                     GKITFLDTPGHEAFTTMRLRGAKATDIVVLVVAADDGVMPQTEESIRHAQAAGVPIIV
                     AVNKIDKPEADPEKIKRQLSEKGLVSEEWGGDVIFNHVSAKQGIGIEALLESILVQAE
                     VLELNASTEARARGVVLEARLDRARGPVCTFLVQEGTLMAGDHIVAGCFSGKVRNMTD
                     WMGTPVAKGTPSVAVEIIGLEGVPDAGDAFYAVESERDAKDIVDNRIIEKRRKVQEAQ
                     TQVTLEDMFTKMKAGEISELSLILKTDVQGSLEAIRDAVLKIGNEEVRARIIHAGVGG
                     VNESDVQLAMASRAIIIGFNVRPEIKALHLAKEHHVDIKMYKVIYDLVNEVKLAMQGL
                     LAPDIKETYLGRAEVRQAFEISKVGTIAGCMVTDGMITRAGRLRLLRDNVVLFEGAIM
                     SLKRFKNDAKDVKQGFECGIGIEGYGDIKPGDVLEAFELTKVQKTL"
BASE COUNT         1318 a         1546 c         1185 g         1587 t
ORIGIN      
        1 cgggctggtc ttggggtgat ggaggctggc gtgtggctgt cgaagtggtt ccggcaccct
       61 cctgtccagt gggctgcctc agtgtgctgt agatgataag tggttggggt tcattcggaa
      121 ccgcggccac caattccgtg attggcatta agaattcttg ttgattctca tttgtctcaa
      181 gctcctcttg ctcatcactc gtctcctctg cattgtcagt ttttaattct tgctcacact
      241 cttcgtacca acaactggca gaataatcct cgctggaaaa acaaacatcg tcttcactgt
      301 aatcactttc gtcctcagac cattcttgct cttcgtacac gtcttcttcg tttgctacga
      361 gctctgcttt ttcttcatct tctcttagca tttcatgata aacactctca atactgtccc
      421 tcacttctcg ctctgccttt tcgaacgaaa aatcatccag tcctgttttt tgagtcactt
      481 ctctggccag cgccttggca ccatcaagca cctcatacac aaaacctttc ggctcttgtg
      541 acccccacat tgtttcgttg tttgttgtgt ctctatcggc cgacacatag ttcctgtatc
      601 cccgcagaag gacattggcg acctcttgcg ctgggccgtt gcccaatatt ttcatcagcg
      661 gcggtttggt gattgccaga aattgatcct gcgcgaagga tgcgtttgac acgtgttctg
      721 cgctcatcag tgttcgcctc gcgacgttat cttctttaaa gtacacatca tcgtcaactc
      781 cgaaggctct cgtagcgagc aaacacgatt tttcggcata ggcctctgaa gcacaagctt
      841 cgaaatcaga ttcctccgcc tcgcatgtgt tttgctcacc cagatcatca tagtcatcat
      901 aacaatagtc agactctatg tagtgtatct cgttgctcat cgggtctttc tttattatcg
      961 gtttagatac cttgaaaatc ctttcattgt agtctttaca attcaatttt gccacgccaa
     1021 caatgacaac aaaagaatta aaaatggggt aatatatacc tgttttacaa gcatggcttt
     1081 gtagcctgaa tcatgaactg ttttatggtc tgttcaagaa ttttttctat tgttggtggg
     1141 gtgttctcgt tttgacaata ataacagtgg tcaagagaca ctggttgcgt cgtttttttt
     1201 atggtttatc aggcgttttg ccctgatgac gtcccgttca agggtcgcca gaccaagaaa
     1261 ctttttatca tgaaagacct tacacacatt gttcgtcagc ccctgagtgg cagacattgc
     1321 tgaaggaggc agctctgaaa tgacacattc cctgccgatc agcttacctt gaaaaagcct
     1381 cttttcacct tcttgattca aataaagact tcgatattgt gacaacatgt ctgctatcgc
     1441 aatctgctgg gacaacaaaa cttcttcctg gtcaagagac atcgcctgtt ccaggcctgc
     1501 accacacaaa cccgtgcgtg acaattcgga caaatacccc atcgtgccaa ggaccttggc
     1561 cagatcactg gccaacacac gtatgtaagt ccccctggaa actgtagtga ctattttgag
     1621 caccggggat tcccaaaaca acacatcaag ctcaaaaaca aaaatcctcc tggcttgtcg
     1681 aggaatagca acgccctgtc tggcaagctc atataatttt ttcccattaa ccttgatggc
     1741 cgaaaacatg gggggcgttt gctgggactc ccccaaaaaa gtcgccatag ccaatacaat
     1801 ctgttcacgc gacaagtatg gaacctctcc tgatgccacc tcgcgccctg tcacatccag
     1861 agaatcggtc tgtttgccaa acacaatcgt ggcctgatag gttttctttt cgtcatcgat
     1921 atagcaaaaa tactttgttg cctcgttcac gccaacaagc aacagacctt ctgcgaaggg
     1981 atcaagggtc ccgtgatggc caattttttt gattttgtgt ttttttttga agatggtcaa
     2041 acacgcctgg gaggtcatcc ccttgggttt gtgtagaaaa agaaaatagg atggttcact
     2101 cattttcgta gagaaaaaag aatctccgtc agaatctgtt ttttgatctc tgtcaggctt
     2161 ccctctatgg tacaaccact ggcccgcaca tgacctcccc cgccaaattt tgcggcgatc
     2221 gcggccacgt ccacaaactc ttttgaccgc atgctgacct tatagacccg agagctcttc
     2281 tgcttgaaca acaccgccac ttcaacccct tcaataaaac ggggataatt gataaatccc
     2341 tcagcgtcct cagacttggc acaagtcacc ttgaaatcat gttgcgtcaa tacgacccag
     2401 gcaatcttgc cgcaggggtg cacttctatc ttgggcatga gccgcttgag cagggccata
     2461 cgtgggagcg agtacatttc aaaacaattt tgcgacacct gccaaacatc cacacccagc
     2521 gccagcaaac gggccgccgt tgtgagtgtt tgtggttttg tgtttgagta tttgaaagac
     2581 cctgtgtccg tggcaatggc cgtgtacaaa ttcgtggcca tctctttggt gatctggaca
     2641 ggcaatttcg caagaaggga ataaatcaac tcgccacacg aagcctgttg cgggcgtgaa
     2701 aaaatataat cagtatcggg gctgcctgta acatggtgat caatgctgat gacggttccc
     2761 agaaatcgat gcgcaagaaa aacatcactc acgcgttttc tctcggccac gtccaacacc
     2821 aaagtgagat caaacttttt gtggagatcc aatttctgac agactgactt ttgcccaggc
     2881 aaaaattgat agatccgcgg caccccatct ttggaataga gacatgtttt cttgcccaat
     2941 tgaaccagcc cctgacccaa agcaatcatc gcccccagcc cgtctccgtc gatattctcg
     3001 tgggtggtga caagaatctg tgaggcaccc agaagggacg aaatgatttt tttctgacta
     3061 atctttttgg ttttggacgt cattgttgat caggggacct cttgacttcc gcaaacagtt
     3121 gatcgacgcg ttcttgcaac gcagccgttt cgtcaaaata aaacacaaga tcgggcatgt
     3181 atttgatgtt gacccgacga gccagttcct ttttgatata ccctttggct ttttgaaacc
     3241 ctttttctac agcgacaaca cgttcacgcc cacccggaat gtcgtaatat accttggcca
     3301 accgcaaatc gggcgtaatc accacatcgg tcacacacag accatcgagc cgaggatcac
     3361 gaacttcgta gagcaaaatg ctcgtaagct ccttctggta ggcctgtgaa atcctgtttt
     3421 gtcttttgtc tttcatgtaa aaaaaggccc tatagcgttt tttgaacttt agtgagttca
     3481 aaagcctcaa gcacatcacc cggtttgatg tcgccataac cttcaatccc tatgccacac
     3541 tcaaacccct gtttgacatc cttcgcgtcg ttcttgaaac gcttcaacga catgatcgct
     3601 ccctcaaata aaaccacgtt gtctctcaac aaccgcaaac gcccggccct ggtgatcatg
     3661 ccatcggtca ccatgcaacc agcgatggtg cccactttgg atatctcaaa ggcttgtctg
     3721 acttcggctc gccccaaata ggtctctttg atgtcgggag ccagcaatcc ctgcatcgcc
     3781 agcttcactt cattgaccag atcgtaaatc accttgtaca tcttgatatc cacatgatgt
     3841 tctttggcca agtgtaaggc cttgatttct ggtcggacat tgaaaccaat gatgatcgct
     3901 cgagaggcca tggccaattg cacatctgat tcattcacac caccaacgcc agcatgaatg
     3961 atcctcgccc tgacttcttc gttgccaatt ttgagaacgg catcacgaat ggcttcgagc
     4021 gaaccctgaa catctgtttt caagatcaag ctcaactcgc tgatctcccc tgccttcatc
     4081 ttggtgaaca tgtcctccag agtcacttgc gtctgtgcct cctgaacctt gcgacgtttt
     4141 tcgataattc tgttgtcaac aatatccttg gcatctctct ccgactcaac agcataaaat
     4201 gcatcaccgg catcaggaac accttccaac ccaatgattt caacggccac agagggcgtc
     4261 ccttttgcaa caggggtccc catccaatca gtcatgttgc gcactttacc ggaaaaacag
     4321 cccgccacga tgtggtcccc tgccatcagg gttccctcct gcaccaggaa cgtacacaca
     4381 ggccctcgcg cgcgatccag tctggcttca agcacgaccc ctctggcacg tgcctctgtg
     4441 ctcgcgttca actccaggac ttccgcctgc accagaatgc tctccaacaa agcctcaatg
     4501 ccaattccct gtttggcaga cacatgattg aaaatcacat cgccgcccca ctcttccgac
     4561 accaaaccct tttcggacag ctgtcgcttg attttttcgg gatctgcttc gggtttgtcg
     4621 attttgttga cggccacaat gatgggaact ccagccgcct gtgcgtgtcg gatagattcc
     4681 tcggtttgtg gcatcacacc atcatctgca gccacaacca gaacaacaat atcggtcgct
     4741 ttggctcccc tcaaacgcat ggtggtaaag gcttcgtgcc ctggcgtgtc caggaaagtg
     4801 atcttgcctt gaggaagaac caccgtgtag gcaccaatgt gctgagtgat gccacccgct
     4861 tcgccactgg caacattggt tgaacggatt ttatccaaca aggatgtttt gccgtggtca
     4921 acatggccca tgatcgtcac cacaggaggc ctcgagggat taccattttc attttctgca
     4981 actgactgct tcgccatgag aagcttgtct tccttaaaag agatatccct tacctcatat
     5041 tgatattctg ccgcgatgag tgtggcggtc tctttatcga tttcttgatt gatcgtggcc
     5101 atgacaccta aatccatcaa cctcttgata atctccccgg ccttgacacc cagcccttgc
     5161 gcaaaattgc ccacagtaat tttctgatcg atctcaacgt agcgcttcga tgttttcttt
     5221 tcggtcagga ctgtttgttt gagattcttg cgggacacga ccttcttttt ctttcgcccc
     5281 atatccgaag gacgaaaaat ccgttcggcc ggcaccaatc ttgataactg agccagggtt
     5341 tgggtgcgcc cgtaaccttc aatgtcaaga tccccgccga tggccttgac acccttgcgt
     5401 gacctgatga gcttcttttg tttttctgcc ttctcctcag ctgtttcgtt gcccgcatca
     5461 acatgtcctg cgaggcccgg ttcaccggca acacgcagtt tctgctcttt cgcgacatct
     5521 tctggttttg tctttttgag atcaatgtgt cccctgattc tgtctttgaa agaaagaatt
     5581 ctgggtcttt cctcctcttt gggtttgggt ttcttcgatg ccttaagttc tgcctc
//
LOCUS       DATDUN010000273         1374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_303662, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000273 DATDUN010000000
VERSION     DATDUN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303662"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VJC18_03760"
     CDS             <1..621
                     /locus_tag="VJC18_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLD44526.1"
                     /translation="TVSSWPIALTAETYLQHQYIEELEKTFLPIAVDIQTGEEIVFRT
                     GKLGDAIRASCSIPGVFGPTLIRGQRLVDGGVRDNVPASLLAEEGADFLVVSHVIPSP
                     QKVRSRREGGFLRDLLSQVWLPGRINDSIRSLYILFREAGGRQAAMADVAFTPDLSRF
                     LPSDFNKSHQIVAEALKQTDQFRREVRDRYRAFCFGKGRDRGQVSG"
     gene            complement(714..>1374)
                     /locus_tag="VJC18_03765"
     CDS             complement(714..>1374)
                     /locus_tag="VJC18_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HLD44527.1"
                     /translation="GMRTPTSPDLLLLPEEEYLLSPTVASKRRLEFTMGRVAAREAIT
                     KLLGTPAPPILKGPNGEPVWPVGIVGSIAHTTEQALAIVAKKHDALSLGIDMERLDRS
                     MDLALTKKLCTPEESNWVHETSNLSEQKLRFIFCAKEAVYKALFPLTGSIRGFLDVAM
                     FPPPGSLAEFQTRAVLQIDWGSGWDQRKEILVNGTTLQNHLVCWVNQRGQTKGVKSLV
                     D"
BASE COUNT          303 a          330 c          380 g          361 t
ORIGIN      
        1 accgtgagtt cctggcccat tgccctgacg gcagagacgt atttgcaaca ccagtatatc
       61 gaagaacttg agaagacctt tttacccatc gctgtcgata tccagaccgg agaggaaata
      121 gtttttcgga caggaaagtt gggtgatgcc attcgtgcca gttgttcgat tcccggtgtg
      181 tttggcccca cactgattcg cggacaacga cttgtagacg gaggggtgcg tgacaatgtc
      241 cctgccagcc tgcttgccga agaaggggcg gattttcttg tggtctcgca tgtgattccg
      301 tctccgcaaa aggttcgctc tcgcagggag ggaggttttt tgagggacct cttgtctcag
      361 gtttggttgc cgggtcggat caatgacagc atcagatcgt tatacatttt gtttcgcgag
      421 gcgggaggac gacaggcggc catggccgat gtggcgttta ctccggatct ctcccgtttt
      481 ttgcccagcg acttcaataa atcccaccag attgtggctg aggcgctaaa gcagacagat
      541 cagtttcgtc gcgaagttcg cgatcggtac cgggcatttt gttttgggaa gggacgtgat
      601 aggggtcaag tctctggttg actagttagt cgtttgttga cctgcttgtg aaacaatttg
      661 cattcttaaa ttaaaggtct ggacatggtg caaaataaag atgttcaaac taactagtca
      721 accagagact tgaccccttt ggtttgaccc ctttggttga cccaacagac cagatgattt
      781 tgcagagttg ttccgttgac cagaatttct tttcgctgat cccaacccga cccccaatcg
      841 atctgcaata cggctctcgt ttgaaactcc gccaaagaac caggaggagg gaacatggcc
      901 acatcaagaa acccccgaat gcttcccgtc agggggaaca aggccttgta gacggcctcc
      961 ttggcgcaga aaatgaatct gagtttttgt tcggataggt tgctggtttc atgaacccag
     1021 ttagactcct ctggtgtgca cagttttttg gtgagagcca gatccataga cctatcgagt
     1081 cgctccatat caattcccaa actgagggca tcatgttttt tggcgacgat ggcgagggcc
     1141 tgttcggtcg tgtgtgcgat actgcctaca atacccacag gccacacggg ttcgccgttt
     1201 ggccctttaa gaatgggagg tgcgggagtt ccgagcagtt ttgtgatcgc ctcgcgcgcg
     1261 gccacgcgtc ccatggtaaa ttcgaggcgg cgtttggacg cgacggttgg agatagtaaa
     1321 tattcttctt ccgggagcaa aagcaaatcc ggcgatgttg gcgttctcat gcca
//
LOCUS       DATDUN010000274         2902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_303688, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000274 DATDUN010000000
VERSION     DATDUN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303688"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /gene="lnt"
                     /locus_tag="VJC18_03770"
     CDS             complement(<1..1233)
                     /gene="lnt"
                     /locus_tag="VJC18_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HLD44528.1"
                     /translation="MEDGRAVSHMKKNVSQQYKPFLVSRTALYGALLTGVLGFLMHPF
                     VVFGYRFPDLGFITWFFLVPLFLGIHRHKFWHKFVLCFAAGTIMGYGKFYWLITAMQK
                     FGGLNFFESFGALTFMCLFLGLLFGFFLALSSWTNHMTKIPLLFIVPLFMTSHDVLLH
                     YFPFNGFPWGLPPYALGEWLAFFQWVDHTGLFGLSLFIYLINGLIAGSFLLFIHRRQL
                     DKAIARFVVMVVLVVLSLFASSLASQQYEKNKTTVKHINLGLVQGNIPQDKKWDPYFA
                     QDILNNYFALTHKAIKDGAQLVIWPETAYPFNLQYPNLFQEQFLNKEKLMTPLLIGAF
                     VTERGDDALHWYNSVVQVNTDASMPRIYRKMHLVPFGEYLPLRRLLSFVPTITRAVGE
                     LTAGHQFDMFTFDDLSIGS"
     gene            complement(1203..1664)
                     /gene="ybeY"
                     /locus_tag="VJC18_03775"
     CDS             complement(1203..1664)
                     /gene="ybeY"
                     /locus_tag="VJC18_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HLD44529.1"
                     /translation="MSLCIQNQHNHLKTSLPCLRKNVRRALEALKLKNTALDIALVND
                     LAMRRLNFEQRNVSTTTDVLSFPSIQPGPDQVGVPRVFSGIFLGDVIISLDVAQKQAL
                     AQHISLSKEVTFLIIHSLLHLLGYDHDTQKKLRTMQAVESRIWRMVALSRT"
     gene            complement(1582..>2902)
                     /locus_tag="VJC18_03780"
     CDS             complement(1582..>2902)
                     /locus_tag="VJC18_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HLD44530.1"
                     /translation="LRFGSFLGGSLQDNTPFPVDITTFYYIIPVAAGAMIVRYILNAE
                     IALIFSIVLSFFAGMFLEHNYSITTYYFLSSITAAHLIGQVEKRSSVLNRGLLLGCGN
                     ALIVLGLSLITNIASATTVDYVALANNTSFAFFGGLITGLTLLAISPVMETIFNYTTN
                     IRLLELADMNHPLLREMIVRAPGTYHHSQLVGVLAEAGTRVIGGNALLARVASYYHDI
                     GKMKKPHYYVENQTNENPHDNLTPSMSALIVESHIKEGMDLAQEHKLPQSIAAFIPEH
                     QGTKLISYFFHKAKMVAGEESGKIDEKDYRYQGPKPQSRESGVVMMADTIEAAVRSLP
                     DKSPQKIRTQVEKLVNNHFVDGQLSNCDLTLRDLYLITEAFIETLIGGIYHQRVEYPE
                     ENKKSQTASLTIIPGLQTKADEPLYPESAQPPENVSPLFKEKRQKGS"
BASE COUNT          747 a          654 c          806 g          695 t
ORIGIN      
        1 ggaaccgatg ctgaggtcat cgaaggtgaa catgtcgaac tggtgtccgg cagtgagctc
       61 accaactgcc cgcgtgatgg tgggaacaaa ggagaggagt cgcctgaggg gcaggtattc
      121 gccaaagggg accaaatgca tctttcgata aatcctgggc atgctggcgt ctgtgttgac
      181 ctgcaccacg gaattgtacc agtgcagagc gtcatcgcca cgttctgtga caaaggcacc
      241 gatcaacagg ggcgtcatca gtttttcttt gtttaaaaat tgttcctgaa acaaattggg
      301 gtattgcaga ttgaagggat aggctgtctc aggccagatg accagctgag caccgtcttt
      361 aatggcttta tgggtgagag caaagtaatt gttgaggatg tcctgcgcga agtaggggtc
      421 ccactttttg tcctgcggga tgttgccttg caccaatccc aaattgatgt gtttgactgt
      481 tgttttgttt ttttcgtact gttggcttgc cagcgaactg gcgaacaggg acaacacaac
      541 caacacgacc atcactacga agcgggctat ggctttgtcg agttgacgtc ggtgaatgaa
      601 aagtagaaaa cttcccgcaa tcaagccatt gatgaggtaa atgaacagag acagaccaaa
      661 cagacctgtg tgatccaccc attgaaaaaa tgccagccac tcgcccaaag cgtagggagg
      721 caggccccag gggaatccgt tgaaaggaaa ataatgcaac agcacatcgt gtgaggtcat
      781 gaacagaggg acgatgaaca acagggggat tttggtcatg tgatttgtcc acgaggacag
      841 agccagaaaa aaaccgaaga gcagacccaa aaaaaggcac atgaaggtga gggcgccgaa
      901 cgactcaaaa aaattcaaac ctccaaattt ttgcatggcc gtgatgagcc agtagaattt
      961 gccgtaaccc atgatcgttc ccgcagcgaa acacagcacg aatttatgcc agaatttgtg
     1021 acggtgaatc cccagaaaca gggggaccag aaaaaaccag gtgatgaatc ccaaatcggg
     1081 aaagcggtac ccgaaaacca caaaggggtg catgagaaaa cccaacacac ccgtgaggag
     1141 ggcaccatag agggctgttc gcgacaccag aaagggtttg tattgttgtg atacgttttt
     1201 cttcatgtgc gagacagcgc gaccatcctc caaattctgc tctccacagc ctgcatggtc
     1261 cgtaattttt tttgggtgtc gtgatcataa cccagcaggt gcaacaggga gtggatgatc
     1321 aggaaggtga cttcctttga tagcgagata tgttgtgcca gagcctgctt ttgggccaca
     1381 tccaaagaga tgatgacgtc ccccaaaaaa attcccgaaa aaacccgggg cacaccaacc
     1441 tggtccggac cgggttgtat cgagggaaaa ctcaacacat cggtggtggt ggaaacgttt
     1501 cgttgctcaa agttgaggcg acgcatcgcg agatcgttga ccagcgcaat gtcgagggcg
     1561 gtgttcttta atttgagagc ctcaagagcc cttctgacgt ttttccttaa acaggggaga
     1621 gacgttttca ggtggttgtg ctgattctgg atacagaggc tcatccgcct ttgtttgaag
     1681 gccgggaatg attgtgaggg atgcggtttg gctctttttg ttttcttctg ggtattcaac
     1741 acgctggtga taaatgcctc caatgagagt ttcaataaaa gcctcggtaa tgaggtacag
     1801 gtcgcgcaat gttaaatcac aattgctcaa ttgtccatcc acaaaatggt tgttcaccag
     1861 tttttcaacc tgtgttctga ttttttgtgg agacttgtct ggcagggaac gcacggcggc
     1921 ctcgatggtg tcggccatca tgacgacccc ggattcgcgg ctttgcggtt tggggccctg
     1981 ataacgataa tccttttcat caattttacc tgattcttct cctgctacca ttttggcttt
     2041 gtgaaaaaag taactgatga gcttagtgcc ttggtgttcc gggatgaaag cggcgatgga
     2101 ttggggaagt ttgtgctcct gagccagatc catgccttct ttgatgtggg attccacgat
     2161 gagcgcgctc atggagggtg taaggttgtc gtgcggattt tcgttggttt gattttccac
     2221 gtaatagtgg ggctttttca ttttgccgat atcatggtaa taactggcca ctctggccag
     2281 cagggcattg cctccaatga ccctggtgcc ggcttccgcc agtacgccta ccagttggct
     2341 atggtggtac gtccccggag cccgaacgat catttctctg agcaaggggt ggttcatgtc
     2401 ggccaattcc aataaacgga tgttcgtggt gtagttgaag atcgtttcca tgaccggtga
     2461 aatggccagc aaggtgagac ctgtgatcaa accaccgaaa aaagcgaacg aagtgttgtt
     2521 tgccagcgcg acataatcaa ccgtggtcgc cgaggcaata ttcgtgatga gacttaaccc
     2581 cagcactatg agagcgttgc cgcaacccag tagcagaccc ctgttgagaa cggacgaccg
     2641 cttttcaacc tgaccaatca ggtgggccgc tgtgatggag ctcaagaaat aatacgtggt
     2701 gatgctgtag ttgtgttcca ggaacatgcc cgcgaaaaaa ctgagcacga tcgaaaaaat
     2761 caaggcgatt tcggcgttga ggatgtatcg gacgatcatg gccccggcgg ccacggggat
     2821 gatgtagtag aaggtggtga tgtctaccgg aaagggcgtg ttgtcttgca gggaaccgcc
     2881 cagaaagctc ccgaaacgca ga
//
LOCUS       DATDUN010000275         1805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_54478, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000275 DATDUN010000000
VERSION     DATDUN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54478"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VJC18_03785"
     CDS             <1..862
                     /locus_tag="VJC18_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44531.1"
                     /translation="FLPTMFPRFVQSTGEHIVSLHPDDIATLQWLYGDGATGTISGEI
                     SDAGGSPVETMLVTVRNTASPLCEAYSQSTSILCSDLNTSPSGDGQAYFNASYCLDGY
                     QLGTYQIPLLENGSYTIDVQEIPEQFSSSIARFDYSVREIPGGAEFYNTLDEANEDTF
                     AITPVNFMQYNLYNKDIVLASTVSGYGDAERVGYAYFETDPLFETTETNDTYCPETGD
                     ITEDMIAETLSQANFAISSDLQEDTQTPIGGCSLRPLNKTHKTQSLSFLCITLFALVC
                     LSTSRLVKSL"
     gene            894..1109
                     /locus_tag="VJC18_03790"
     CDS             894..1109
                     /locus_tag="VJC18_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44532.1"
                     /translation="MKKWFLLLFVSCFLLYKYVLPKIDAEISQGYYEDWGQPQQEVKK
                     EGTWDKAKKFFENLFTDKNKKEKFIYK"
     gene            1487..>1805
                     /locus_tag="VJC18_03795"
     CDS             1487..>1805
                     /locus_tag="VJC18_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLD44533.1"
                     /translation="MFALIGFSLLSTLTTAQTVPTASPLANQIEHLAEEFNPQVVNWR
                     RNFHQHPELGNGEVRTSKIIADELRKLGFEVTNGIAKTGVVGVLRGGKPGPVIALRAD
                     MDAL"
BASE COUNT          483 a          491 c          394 g          437 t
ORIGIN      
        1 ttttctgccc accatgttcc cccgctttgt tcagtcgaca ggagaacaca tcgtgtcgtt
       61 gcatcccgac gatatcgcca cactccaatg gctctacgga gacggagcaa ccggcaccat
      121 ctccggagaa atcagcgacg ccggtggttc tcccgtggaa accatgctgg tgactgtgcg
      181 caacaccgca tctccccttt gcgaagccta ctcgcagtca acgagcattt tgtgttcaga
      241 cctcaacacg tccccgtcgg gcgatggaca agcctacttt aatgcgtcgt actgtcttga
      301 tggctatcaa ctgggaactt atcaaatccc cttgttggaa aatggttcgt acaccatcga
      361 tgtccaggaa atcccagaac agttctccag ttcaatcgca cgctttgatt acagcgttcg
      421 cgagatcccc ggtggggccg aattttacaa caccctcgat gaggccaatg aggatacctt
      481 cgccataacg cccgtcaatt tcatgcaata caacctctac aacaaagaca ttgtgctggc
      541 ctctacggtc agtgggtatg gcgatgccga acgcgttggc tatgcctatt tcgaaacaga
      601 tccccttttt gaaactaccg aaacaaatga cacctactgt ccagaaacgg gggacatcac
      661 agaagacatg atcgcagaga ccctcagtca ggccaatttc gcgatttcct ccgatcttca
      721 agaggacaca cagaccccca tcggaggttg ttctctcaga cccctgaaca agactcataa
      781 gacacaatct ctctcgttcc tctgcatcac cctgtttgcg ttggtgtgct tgtcaacgtc
      841 caggctcgta aaatcccttt aatccaaatg cgaatagtgt tagaatcgca cctatgaaaa
      901 aatggttttt acttttattt gtctcctgtt ttctcttgta caaatacgtg ctccccaaaa
      961 tcgacgccga gatttctcag gggtattacg aagactgggg acaaccacag caggaggtca
     1021 aaaaggaagg gacctgggac aaggccaaga aattctttga aaacctgttc accgacaaaa
     1081 acaaaaaaga gaaattcatc tacaagtaaa gcagatagat agacagacta ttttttgaca
     1141 gactattttt tggaccccca ctaatctcgc accacccttt cccacttttt cagaggaggc
     1201 ggggtcaggt cttgactagg gacatgcctg ccacagatgg tcaatgagcc acatactcac
     1261 tgcatgtccc tagtcaagac cccgctaccc cgctaccccg ctatttaaag tttataaagt
     1321 tcctaaagaa atcgttcttg cctatccggt tgccaaagtg tactttggcc ccagtttcga
     1381 ctgtcaacag tttgatcaaa attgcaccca acacaaataa aaaccttggc gaaattttaa
     1441 agatcgattt tagatgccta ttggagactt caaaatgaga actttcatgt ttgccctgat
     1501 cgggttttcg ttgttatcaa cccttacaac tgctcaaacg gtcccaacgg ccagtccatt
     1561 ggcaaaccaa atcgagcacc tggcggagga gttcaacccg caggtggtca actggcggcg
     1621 caattttcac cagcatcccg aattgggaaa tggtgaggtt cgcacatcga aaatcatcgc
     1681 agatgaactg cgcaaactcg gcttcgaagt gactaatgga attgcaaaaa ccggtgtcgt
     1741 cggcgttctg cgcggaggca agccaggtcc ggttatcgcg cttcgggcag acatggacgc
     1801 actgc
//
LOCUS       DATDUN010000276         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_54545, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000276 DATDUN010000000
VERSION     DATDUN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54545"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1460)
                     /locus_tag="VJC18_03800"
     CDS             complement(156..1460)
                     /locus_tag="VJC18_03800"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HLD44534.1"
                     /translation="MENIVSLCKRRGFIYQSSEIYGGINGFWDYGPMGVELKKNVKNF
                     WWRTMVDLRDDVVGVDTTIIAHPETWRASGHVASFSDPMVDCRLCKGRYRADQLEKDA
                     NGKYLCPTSKCRGDFTEARAFNLMFRTFVGAQEDSSSEAYLRPETCQSIFTQFKNVLT
                     TSRQKVPFGIAQIGKSFRNEITPRNFTFRSREFEQMEMEFFIKPDTAVQEEWYEYWKN
                     TRYQWYHDLGIKKESLHLRDHEKDELAHYASACVDVEYDFPFGRSELEGIANRGAYDL
                     SQHIQFSKKDLSYFNQETNEKFVPAVIEASAGCDRTVLTVLCDAYHEDEVDGEKRVVM
                     SFAPHIAPIKIAILPLSKKLAEPAKKIEAELRRKVRTDYDDAGSIGKRYRRQDEVGTP
                     FCATYDFDSETDQKVTIRHRDTMEQQRVAIGEIEEYLRERGI"
     gene            1712..2179
                     /locus_tag="VJC18_03805"
     CDS             1712..2179
                     /locus_tag="VJC18_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44535.1"
                     /translation="MLVLPAEEMATLKTCHFEKLSFETKDFEQITASRLELKTTIWAL
                     ELFKTDAKALKLTVCNNFRATKDLLQRREKLELTNYAGFRPATTITNAVLFQQFFKLN
                     DRLHFDVLWMGEPAEDAKKTTPLQPLFKEVVKRADQALKSQLKAQNPVADKWF"
BASE COUNT          570 a          605 c          473 g          626 t
ORIGIN      
        1 ttttttctgg attgtttttt tgctcttttt cattttttat ttcctttgat tcatgtgtat
       61 tgaaacttgt attcgagtgt atcgataaca cctgtggcca ttgacttcat taacctaacg
      121 tcaacacgta cgcacgccaa cacgtacgca cgtatttata tcccccgttc cctcaaatat
      181 tcctctatct caccaatcgc gacccgctgt tgttccatgg tatcccgatg acggatagtg
      241 actttttgat ccgtttccga gtcaaaatcg tacgtggcgc aaaagggagt ccccacctcg
      301 tcctgacgac ggtagcgctt tccgatcgaa ccggcatcgt cgtaatcggt tctgactttg
      361 cgcctgagct ctgcctcaat tttcttggct ggctcagcca gtttttttga aagcggcagg
      421 atggcaattt tgatgggcgc gatgtgcggc gcaaaactca tcaccaccct cttttcccca
      481 tccacttcgt cttcatggta cgcatcacaa agcactgtga ggaccgtcct gtcacaaccc
      541 gctgacgcct caatgacggc gggtacaaat ttttcgttgg tttcctggtt aaaatagctc
      601 aggtcttttt tggaaaactg gatgtgctga ctcagatcgt aggcccctct gttggcaatg
      661 ccttcgagtt cgctccggcc aaagggaaaa tcgtattcca catcgacaca ggcactggca
      721 tagtgggcca actcgtcttt ttcgtgatcc ctgaggtgca aactttcttt tttgataccc
      781 agatcatgat accactgata tcgcgtgttc ttccagtatt cataccactc ctcttgcaca
      841 gccgtatccg gcttgatgaa aaactccatt tccatctgtt caaactcgcg tgaacgaaac
      901 gtgaaattgc gcggggtgat ttcgttacga aagctcttgc caatctgagc aatgccaaac
      961 gggacttttt ggcgcgatgt ggtcagcacg tttttaaact gcgtaaaaat cgactggcag
     1021 gtctccggcc gcagatacgc ctcgctggag ctgtcttcct gcgcccccac aaacgtcctg
     1081 aacatgagat tgaacgcacg tgcctccgta aaatcgccac ggcatttgct ggtgggacaa
     1141 agatatttgc cattggcgtc cttttcgagc tggtccgcgc ggtagcgacc cttgcacaac
     1201 cggcaatcga ccatgggatc cgaaaaggag gccacatgcc cgctggcccg ccacgtttca
     1261 gggtgcgcga taatagtggt gtcgaccccg accacatcgt ctctcaggtc gaccatcgtt
     1321 cgccaccaga aattcttgac atttttcttg agttccacgc ccatgggacc atagtcccaa
     1381 aacccgttga tgccaccata gatttcactg ctttgataga taaagcccct tcgcttgcac
     1441 aacgacacaa tgttttccat gccattacct cattattata ttcattagtg gcgaattgat
     1501 taccaaaaag acgcagacct ttacaataaa tttattgtat agtccagagt attggggtgg
     1561 caatttgggc tgaatgccag cgaacacttg ccataacgag gagaatcggg tacgaaaaac
     1621 ccctatgcct cttacaacta ccaatactag caaatgtatt cttttcactg cctgttgttt
     1681 tgacccccgc ttaaaactgg gaacgggttg catgcttgtt ttacccgctg aagaaatggc
     1741 cacccttaaa acctgccatt ttgagaaact ctctttcgaa acaaaggact ttgagcaaat
     1801 cacagcctcg cgtctggaat tgaaaaccac aatctgggcc cttgagctct ttaagaccga
     1861 tgccaaagct ctcaaattga ctgtttgcaa caatttcagg gccaccaagg atcttttgca
     1921 aagacgagaa aaactcgaac tcaccaacta cgccgggttc agacctgcga caacgatcac
     1981 caacgctgtg ttgtttcaac agttcttcaa gcttaacgac cggttgcatt ttgatgttct
     2041 ttggatgggt gagcctgcag aagacgcaaa aaaaaccaca ccactacaac ccctgtttaa
     2101 agaggttgtc aaaagagccg accaagccct caaatcccaa ctcaaggccc agaatcctgt
     2161 cgccgataag tggttttaaa tgactatgct tttgaatcaa atatttccgg tcattgtcgt
     2221 cattgtatca gcaaaacctt ataaaacctt attaataaac cttataaacc ttat
//
LOCUS       DATDUN010000277         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_179449, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000277 DATDUN010000000
VERSION     DATDUN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179449"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..858
                     /locus_tag="VJC18_03810"
     CDS             175..858
                     /locus_tag="VJC18_03810"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HLD44536.1"
                     /translation="MFIKSHNLAHKGFTLLEIVIAMSILAVMMVSITQLTGSILVKKD
                     RNEIRMQLQHQITVAITKITDDLHMAFLADSKFQGKDQKYKTGFVGKEDGVNFSTMSQ
                     VHFVKNKADTEQVAVGYFLEKNEADTFVLKRRQTDRLPEKLDVGGQAFALLRDVSQFS
                     LEYFDANKKEWQKQWDTESVSAGGQLPRRVKIKIAIMGESIDEEEQRKEYNFEFLVEI
                     PMFATKINF"
     gene            871..2211
                     /locus_tag="VJC18_03815"
     CDS             871..2211
                     /locus_tag="VJC18_03815"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44537.1"
                     /translation="MNKKGAAIILVLAAIMLLTVVCLDMFDDSLVSHQLAQNYRARTQ
                     AYYLAKSGVNFSKMLLFYNKQVESELTKKKTSLQALGYEPLYKMIPLSSEALRGMVSM
                     GAMLSGGEEGEVTDGTEDEAEAEAEAEEDKDESENEGDASTADSVGMLQKEGIEEFLG
                     FEGNFDVEISEEQSKYSLNAVSKMTSNSPSYDLHKKVLLSLLLRPEFKNFFNNQAQEA
                     ETLTHAIADFVDANNVINEFDQVERGDEGSAYNKLSYQAKDGPFLTLSELRLVPGMGD
                     DIFETIKPLVSVYHTSEKVNVCLAEPEIVDALIAHYTKYSECTTPLDGEKDADEIAKH
                     RDELLALCPDKTAMATALNVALGISSAADANTATTTSSASGTTTNVSSATGKTSSKVA
                     GCQIQFEDLIATDNNVFRIKASGSVGDITSSIVIVLDTSGGKANQWPVLYYRID"
     gene            2258..>2657
                     /gene="pilM"
                     /locus_tag="VJC18_03820"
     CDS             2258..>2657
                     /gene="pilM"
                     /locus_tag="VJC18_03820"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HLD44538.1"
                     /translation="MSQSILGIDLGTYSIKVLRLERRLQEIHLLEFFELPLNLHVRHQ
                     HHEQVAEVLEPFFKEHQFSADIICLNVPGHTLSNRILEIPFTNAKKIAQSIEFELEGH
                     IPFPIEEVFWDYHVLSQGESSSQVLCAYAYE"
BASE COUNT          746 a          553 c          690 g          668 t
ORIGIN      
        1 atcaaataac gagcttacga aagacgaaaa cacgagggac gaaggacgac aggacgaaga
       61 aaagcaaaat gtcagccgca ggaatgatcc cgctttgcgg gcaaaatgaa aagcaaaggg
      121 tttttttcct ttaagctttg agctttcacc tttgagctga tttcgatcca cgctatgttt
      181 ataaaatcac acaacctcgc ccacaaaggc ttcactctgc tggagatcgt gattgcgatg
      241 agcatcctgg ccgtgatgat ggtgtccatc acacagctca cgggaagtat tttggtcaaa
      301 aaggatcgca atgagatcag gatgcaattg cagcaccaga tcactgttgc catcaccaag
      361 atcacagacg atttgcacat ggcttttttg gcagatagca agtttcaggg caaggaccaa
      421 aagtacaaga caggttttgt gggcaaggaa gatggtgtga atttttccac aatgagtcag
      481 gtccattttg taaaaaacaa agccgatacc gagcaagtcg ctgtggggta ctttttggaa
      541 aagaacgaag cagacacatt tgttctcaag agacggcaaa cagaccggtt gccggaaaag
      601 ctggatgtcg gtggccaggc ctttgctctc ttgcgagatg tgagtcagtt cagtttagag
      661 tattttgatg ccaacaaaaa agagtggcag aaacaatggg acacggaatc cgtgtcggcc
      721 ggcggtcaat tgccccggag ggtaaaaata aagatagcga tcatggggga gtccatcgat
      781 gaagaggaac aacgaaaaga gtacaacttt gagtttctgg ttgaaattcc catgtttgcc
      841 accaaaatca atttttagat gaaaacacga ttgaacaaaa aaggagccgc catcattctg
      901 gtcttagccg ctatcatgct gctcaccgtg gtgtgtttgg acatgttcga tgattctctt
      961 gtgagtcatc agttggctca aaattacagg gcgcggacgc aggcttatta tctggcgaaa
     1021 tcaggcgtca atttttccaa gatgttgctt ttctacaaca aacaggttga gagtgaattg
     1081 acaaaaaaga agacgagttt gcaggcattg ggatatgagc cgttgtacaa aatgatccca
     1141 ctcagcagtg aggccttgcg cggcatggtc tccatggggg cgatgctgtc aggaggagaa
     1201 gagggcgaag tgactgatgg gacagaggat gaggctgaag ctgaagctga agcagaggag
     1261 gataaagatg aaagtgaaaa tgagggtgat gcctctacag cagacagcgt gggcatgttg
     1321 caaaaagaag ggattgagga atttttgggt tttgagggaa atttcgatgt ggagatcagc
     1381 gaagagcaat ccaaatattc tctgaacgct gtttccaaga tgacatcgaa ctcacccagc
     1441 tatgatttgc acaaaaaagt cttgctgtcc ttgctcctgc gccctgaatt taaaaatttc
     1501 ttcaacaatc aagctcaaga ggctgagacc ctgactcacg cgattgctga ttttgtcgat
     1561 gccaataatg tgatcaacga gtttgaccag gtggaacgag gggacgaagg gtctgcctat
     1621 aacaaattat cataccaggc caaggatggc cctttcttaa ccttgtctga actgcgtctg
     1681 gttccgggaa tgggtgatga tatttttgag accatcaaac ccctggtttc ggtttatcac
     1741 acgagcgaaa aagtgaatgt ttgtcttgcg gagcccgaga tcgtcgatgc cctcatagcc
     1801 cactatacaa aatattccga gtgtacgacg cctcttgacg gggaaaagga cgctgatgaa
     1861 atcgccaagc atcgcgatga gcttctggcc ctttgtcccg acaaaacagc gatggctacg
     1921 gcgttgaatg ttgctttggg aatcagttcg gctgccgacg caaacacagc aacgacgaca
     1981 agctctgcct ccggcactac caccaatgtc agttcagcga cgggaaagac atcgtcgaag
     2041 gttgcgggtt gtcagattca attcgaagat ctcatcgcga cagacaacaa tgtctttcgc
     2101 atcaaggcat caggctccgt tggcgatatc acctcatcaa ttgtgattgt tctcgatacg
     2161 agtggtggca aagcgaatca gtggccggtt ctgtattatc ggatcgattg attatttcct
     2221 ttgtaatttt atcaacttgt aatactatgg cggggagatg tcccagagta tattaggcat
     2281 tgatctcggc acttattcga tcaaggttct caggctggaa aggcgattgc aggagattca
     2341 tttgttggaa ttttttgaat tgcctctcaa tctgcatgtg cgacaccagc atcatgagca
     2401 ggttgccgaa gtattggagc cgtttttcaa ggaacatcag ttttcagcag acattatctg
     2461 tttgaatgtt cccggccaca cgctgtcaaa tcgcattttg gagatcccct ttacgaacgc
     2521 gaaaaaaatt gctcaatcta tcgagtttga gctggaaggc cacatccctt ttcccattga
     2581 ggaagttttc tgggattatc atgtgctgag ccagggagag agcagttcgc aggtgttgtg
     2641 tgcctatgct tatgagg
//
LOCUS       DATDUN010000278         4189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_139166, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000278 DATDUN010000000
VERSION     DATDUN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139166"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..345)
                     /locus_tag="VJC18_03825"
     CDS             complement(157..345)
                     /locus_tag="VJC18_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44539.1"
                     /translation="MTKKFDCVDMKHIAAKEIYKETKDMTLEQELEYWTEQANELRKL
                     MESKKKKSSQTTHITSGF"
     gene            complement(338..796)
                     /locus_tag="VJC18_03830"
     CDS             complement(338..796)
                     /locus_tag="VJC18_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002787576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLD44540.1"
                     /translation="MKRPRVYVDTSVYGGVFDDEFKDVSGIFFSQVQKGTFLLVTSPT
                     VKNEIDRAPEQVKIFFDSFMSQAEMAIVTNEVLQLRNAYLDAGIVSQNYSDDALHVAL
                     ATVALCQIIVSWNFKHIVHYDKIDLYNAINTKNGYNRIAICAPAEVIKYD"
     gene            981..2420
                     /locus_tag="VJC18_03835"
     CDS             981..2420
                     /locus_tag="VJC18_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44541.1"
                     /translation="MIDLATNEFHETKQPFSDLLGAEVDQATYLKQAAQKLALLNSLY
                     DPAQNVETQTEHFLGLLSDRIFLPSPAILRHITSNIPVLFDDVAFLLSDDTDHIFDTL
                     KRASLFLQNHTRVTIDFSALRAKRSGAALGNKQFVGPIRLMELFERTPQPSETPTKLN
                     FTLSLDHLEIDDCLHYIRTAPKHIEYTIGVPNQFVSALARKERFFLRHQPHAEPSTSI
                     DAIKLFHKLINLIRDDHPIDLVFTDKINAFKQQRALPTNLILNGLHQLVWPGELMATG
                     VIDLTLLTEELTDHGDKLRETMARVINFLDNTFDLNHYFDKDSEALTTRTRRLGISIN
                     GLDTLLTKMNPEHSPNKNQHITSKLAHTIKETLIEASHKLGQQRGYVTQVSFANHPIK
                     TRNTQLLAQIHDPLLANELDATNFLMTSPAPLTQYPEHYDTHLRWQEVCDNLVSLPHP
                     LHHTDLDTFVKLFFLAFEKGFLSFETVRA"
     gene            2676..>4189
                     /locus_tag="VJC18_03840"
     CDS             2676..>4189
                     /locus_tag="VJC18_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLD44542.1"
                     /translation="MESAVKIDQRYEIIKNLGGGLSGEVLLVKDQEGIKALKFLKKIQ
                     MNVSREDALRNFKNEFSILKELNHPNIARILDFGFETKLQKYYFTTEFIQGAELHTAC
                     ENQPLDVLEKIIVQVLRALNYLHSRGIYHFDIKPQNILVYTEGGVPVTAKIIDFGLAG
                     FSASRKKVGTPAYMAPEVVQGGLLDGRTDLYSFGVLLYKILTGTNPFATKDLKEILHN
                     QVHLIAKPPSTVKSDLPSYWDHIVGRLLEKNPLNRYSQASLVIRDLNFLSTKKFDIET
                     KDTKLSYLPEKGTLIGREEEWQTFAEMFAETFLDDELPAEKTLIVEGGRGTGKTRLLS
                     EIKYFSQLRNVPAKTLKQFESEERANAYLLMLDDPAIDTSRVNALIQETANEKCLLIW
                     ATETAPKGWANTRTLTLKNYDKPQLKQYLESVTGLSSAPDRLIDEVYKRTQGTPLFVT
                     EFIKLVLDNDLLYDASGKWDATTFEDIKIDFNRIHIPNSVEEILIDKFHKLESS"
BASE COUNT         1149 a         1178 c          861 g         1001 t
ORIGIN      
        1 gctaacgctc agggagcccc tccttatcac cgcaaggcgg gatcccgttt gcgggacaaa
       61 aaggaggggc ggtgccctcc tgttgcttgt tgcgcaataa gatttcaaag agaaaagcct
      121 tttttaccca cttttatgtc gcacacccga taccctttag aatcctgatg taatatgagt
      181 tgtttgagat gatttctttt tcttggactc catcaacttt cgcagttcgt tggcttgttc
      241 cgtccaatat tccagttcct gctcaagagt catatccttt gtctctttgt aaatttcttt
      301 tgccgctatg tgcttcatgt cgacacaatc aaatttttta gtcatacttg atcacctccg
      361 ccggcgcaca aatggcaatt ctattgtagc cattcttggt attgatcgca ttgtacagat
      421 caattttgtc gtaatgaaca atgtgtttaa aattccagct cacaatgatt tgacacaatg
      481 ccactgtcgc caacgcgaca tgcagagcat catccgaata gttttgagat acgattcccg
      541 catccaaata agcatttcgt aattgcagga cttcgttggt gacgattgcc atttcggctt
      601 ggctcatgaa tgaatcaaaa aaaattttga cttgttcggg agcacgatca atctcatttt
      661 ttaccgtagg tgaggtgacc aaaagaaacg ttcctttctg aacttgcgaa aagaaaatac
      721 cactcacatc tttaaattcg tcatcaaaaa ccccaccgta aacggacgta tcaacataca
      781 cgcgtggtct tttcatacct cgactccttg tgcgcaaaat tataccaata aaactgttac
      841 gcgatcaata accaaataat ccgaataaaa tcacgccgta gcgttaatgc tgtgttgctc
      901 aatgcaacag ggccaccagc tcatctttgg agacgggggt attgccaaga acagggatct
      961 gtgacataca aaatccaaat atgatcgatt tggccacaaa cgaattccat gagacgaaac
     1021 agcctttttc tgacctcttg ggagcagaag ttgaccaagc cacatacctc aaacaggcag
     1081 ctcaaaaatt ggctctgctc aattctttgt atgaccctgc ccaaaacgtg gagacccaaa
     1141 ccgaacattt tttggggtta ttgtctgatc ggattttcct gccctccccc gcgatcctgc
     1201 gccacatcac cagcaacatt cctgtgctgt ttgatgacgt ggctttcctg ctttccgacg
     1261 atacggatca catcttcgat accctcaaaa gggcttcgct ctttttgcaa aatcacacgc
     1321 gcgttacgat tgatttttct gccttgaggg ccaagcgatc aggggccgca ctgggaaaca
     1381 aacaatttgt cgggcccatc cgtctgatgg agctttttga acgaacccct cagccgtctg
     1441 agactcccac caaactcaat tttaccctga gcctcgacca tctcgagatt gatgattgcc
     1501 tgcattacat cagaaccgcc ccaaaacaca tcgaatacac gattggcgtg cctaaccagt
     1561 ttgtcagtgc tctggcgcgt aaagagagat tttttttaag gcatcaaccc catgccgaac
     1621 cgtcaacatc catcgacgcc atcaagttgt ttcacaaact catcaacctc attcgcgatg
     1681 atcaccccat cgatcttgtg ttcacagaca aaatcaatgc cttcaaacaa caacgagccc
     1741 tgcctaccaa ccttatcctg aatggcctcc accagttggt ctggccggga gaattaatgg
     1801 caactggggt gatcgatttg acgctcctca cagaggagct cacagaccac ggagacaaac
     1861 tgcgagagac aatggcacgg gtcatcaatt ttctcgacaa cactttcgat ttaaatcatt
     1921 acttcgacaa agattctgag gcactcacaa cccgcaccag acgtctgggc atctcgatca
     1981 atggcctcga tacactgttg acgaagatga acccggaaca cagcccaaac aaaaaccaac
     2041 acatcaccag caaactggct cacacaatca aggaaacact cattgaggcc tcacacaagc
     2101 tgggacagca acgcggttat gtgacacagg tgtcgtttgc caaccacccg atcaagaccc
     2161 gaaacacgca gttattggct caaatacacg atcccctgct tgccaacgaa cttgacgcca
     2221 cgaacttcct catgacctcc cccgcccctc ttacacaata tccagaacac tacgacaccc
     2281 acctgcgctg gcaagaagtc tgtgacaacc tcgtctctct gcctcacccc ctgcaccaca
     2341 cagatctcga tacctttgta aaactgtttt tcctcgcctt tgaaaaaggt tttttgagtt
     2401 tcgagaccgt gcgggcttga tcagtcatga ttgtcaatgc ctgttaagac ctgtcatgct
     2461 ttgtcctgcc acggcttgtc cttagatcaa aaaaatcgtc acgccatcat catgaatcgt
     2521 atgagtgggg cctcttattt atatttcata tatacaccga gaccattcat cttgtataat
     2581 ataagttgat ttacattctc tcaggacatg tctacgggtc ctgcctgaga gaatggcggc
     2641 acacgatggt tgtttgggga gggggttagg tcaacatgga atcagccgtt aaaattgatc
     2701 agcgctacga gatcatcaaa aacctgggcg gcggcctgtc gggagaggtc cttctcgtga
     2761 aagatcagga agggatcaag gccctcaagt ttctcaaaaa aatccagatg aacgtctcac
     2821 gcgaagacgc cctgcgcaat ttcaaaaacg aattctcaat tttaaaagaa ctcaatcacc
     2881 ccaacatcgc ccgcattctg gattttggtt ttgaaaccaa actgcaaaaa tattacttca
     2941 ccaccgaatt cattcaggga gcagaactcc acacggcctg cgaaaaccag ccactcgatg
     3001 ttctcgaaaa aatcatcgtg caggtcctca gggcgctcaa ttacctgcac tcgcgaggga
     3061 tctatcactt cgacatcaaa cctcagaaca tcctcgtgta cacggaaggc ggggtccctg
     3121 tcaccgccaa gatcatcgac ttcggtctgg ccggtttttc ggcttcccgc aaaaaagtgg
     3181 gtacccctgc ctacatggcc cccgaagtag ttcaaggggg tcttctcgat ggacgcaccg
     3241 atctgtactc ctttggcgtg ctcctctaca aaatcctgac gggcaccaac ccgttcgcca
     3301 ccaaagacct caaggaaatc ctgcacaatc aggtgcactt gatcgccaag ccgccctcaa
     3361 cggtcaaaag cgacctgccg tcgtattggg accacatcgt gggtcgactg ctggagaaga
     3421 atcctctcaa ccgctattcc caagcctcgc tcgttattcg cgatttgaat tttctatcga
     3481 ccaaaaaatt cgacatcgaa accaaagaca ccaaactcag ctacctgccc gaaaaaggca
     3541 ccctcattgg gcgcgaagaa gagtggcaga cctttgccga gatgttcgcc gaaacctttc
     3601 tggacgacga actccccgcg gaaaaaaccc tcatcgtcga gggtgggcgc ggaacaggca
     3661 agacccggct gttgtcggag ataaaatatt tttcgcaact gcgaaacgtc cctgccaaaa
     3721 ccctcaagca attcgaatcc gaggagaggg caaacgcgta cctcctcatg ctggacgatc
     3781 ccgccatcga caccagtcgg gtcaacgccc tcatacagga gacagccaac gaaaaatgcc
     3841 tgctgatctg ggctaccgaa acagccccca agggttgggc caacacacgc accctcacac
     3901 tcaagaacta cgacaaacca caactcaaac agtatctcga atcggtcaca ggtttgtcct
     3961 cggccccgga cagactcatc gacgaagtct acaagcgcac acagggcacc cctctttttg
     4021 tgaccgaatt catcaagctc gtcctcgaca atgatctctt gtacgatgca agcggcaagt
     4081 gggatgccac cacctttgag gacatcaaga tcgactttaa caggatccac atccccaaca
     4141 gcgtggagga aatcctgatc gacaaatttc acaaactcga atcctcgca
//
LOCUS       DATDUN010000279         1131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_303940, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000279 DATDUN010000000
VERSION     DATDUN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303940"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..>1131)
                     /locus_tag="VJC18_03845"
     CDS             complement(316..>1131)
                     /locus_tag="VJC18_03845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HLD44543.1"
                     /translation="TPIKFYQMAQALNTKSPTYIRANIKKIPEEKLILKFKEDNIPCF
                     QTSVSNCLLLPELSISPKDIPGYHEGLWTIQNMASQRIVRYLEIKDGDDILDACAGYG
                     GKTLQMAEILKDKVNIHYFDTIDKKMHAIDVRAQKMGFRHVKPVMQFEPIYNRMLIDA
                     PCSGLGTISSHPEIKWFRTPEDLQEQSKKQLEVLNTYSQYLKPGGTLLYAVCSIDKNE
                     GEEVVKTFLGEHAEFERDKSLSTPFLDTEFGSYLLPTDKGESGYFIARFLKKA"
BASE COUNT          272 a          265 c          275 g          319 t
ORIGIN      
        1 atgacgacca gacgttcgaa accgctggca tcgtaggttt tggccttgta cacctgcgcc
       61 ataccgccct gggcaatttt ttcgatgaga gtgtactttc cgagtcgttt ttgattctgg
      121 tcaatcatga ctaaaaggtc acatgaatac cgaatagcga tcaaggaatg tcgaatcctt
      181 caacaacgtt caggacaagt ccttcaacaa cgttcaggac aagtgttgaa gtgagggttt
      241 cttgttctac atttcagcca taggactgat cccgctttgc gggacattat tcgatattca
      301 aagatgcttc tcgcatcacg cttttttcaa aaaccgtgca atgaaatacc ccgactctcc
      361 tttatcggtc ggcaataaat acgatccaaa ttcggtgtcc aaaaagggcg tgctcagcga
      421 tttgtcgcgt tcaaactcag cgtgttcacc cagaaaggtc ttcaccactt cttcgccttc
      481 gtttttatcg atcgaacaca cagcgtaaag caacgtgccg cccggtttga gatactgact
      541 gtacgtattc agaacctcaa gctgtttttt gctctgttcc tgcaaatcct ctggagtacg
      601 gaaccatttg atttcgggat gggatgatat ggtgcccagt ccggaacagg gcgcgtcgat
      661 gagcatccgg ttgtagatgg gctcaaactg catgacaggc ttgacgtgcc tgaaccccat
      721 tttttgcgca cgcacatcga tggcgtgcat tttcttgtcg atggtatcga aataatgaat
      781 attgaccttg tctttgagta tctccgccat ttgcagagtc ttgccgccat accccgcgca
      841 ggcatcgaga atatcgtcac cgtccttgat ctcgaggtag cgaacaatcc gttgactggc
      901 catgttctgg atcgtccaca gaccttcgtg gtaaccgggg atgtccttgg gagaaatcga
      961 aagttcgggt aaaagaaggc aattggagac agaggtctga aaacaaggga tattgtcttc
     1021 cttgaattta aggatgagtt tttcctccgg gattttcttg atattcgcgc ggatgtacgt
     1081 tggtgatttg gtgttcagcg cctgagccat ctgataaaac ttgatggggg t
//
LOCUS       DATDUN010000280         1297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_303950, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000280 DATDUN010000000
VERSION     DATDUN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303950"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VJC18_03850"
     CDS             <1..1061
                     /locus_tag="VJC18_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44544.1"
                     /translation="ICGDVINNNRVDKPYWFLRVIEAKFRLNDIESAIQYGVRYMQMF
                     PDIKTSFFMGNPENYAKQGLAPAIHYVLGLSLDRMNLFGDHVYNNIAFGMDKITDEKE
                     KILLTAFAWLEMRQYEKAIPLYADYIDKSASEIRDVYFSDQAVDLDSQTFKLAEQQLL
                     DTLQLKGNHPQTLIMLGNIWMGRTGNPGYMTQAHHCFDRAIKLKPDYMEAWHHQGLAY
                     VVDNNMEQAIAHLGHSISLHAQTQNSALVGYADFVASIYLISKDRLSKTVSPEAAHYK
                     ALFICASSQWQGEQTEPARDNLQRIKEDLNKLDASTLTREIRDVFAQMLALLPATMKQ
                     DERAQVLIRDIEWKLKNT"
     gene            complement(1086..>1297)
                     /locus_tag="VJC18_03855"
     CDS             complement(1086..>1297)
                     /locus_tag="VJC18_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44545.1"
                     /translation="RDGKTHQIESLMQMGKAFGNMLLNGSLQELVKKGLVDATDAYYK
                     SVDKEDMLKKLQEIGVKLDTSKLTN"
BASE COUNT          340 a          301 c          319 g          337 t
ORIGIN      
        1 gtatctgtgg tgatgtcatc aacaataaca gggtcgataa accttactgg tttctcaggg
       61 tgatcgaggc caaattcaga ttgaatgata ttgagtcggc cattcaatat ggtgtcagat
      121 acatgcaaat gtttccggat atcaaaacgt cattttttat gggcaacccg gaaaattatg
      181 ccaaacaagg cttggcgcct gccattcatt atgtgctggg gctctccttg gaccgcatga
      241 atctctttgg agatcacgtg tataacaaca ttgcttttgg catggacaag ataaccgatg
      301 aaaaagaaaa aattctattg acggcctttg cctggttgga aatgaggcag tacgaaaagg
      361 ctattccgct gtatgctgac tatatcgata aatctgcctc tgagatcaga gatgtctatt
      421 tttctgacca agcggttgat cttgacagcc aaaccttcaa gttggctgaa caacagttgc
      481 tggataccct gcaactcaaa ggcaatcatc cacaaacctt gatcatgctt ggcaatatct
      541 ggatggggcg cacgggcaat cccggctaca tgacacaggc acatcattgt tttgacaggg
      601 cgatcaagct caagcccgat tacatggagg cgtggcatca tcagggtttg gcctatgttg
      661 tcgacaacaa tatggagcag gccatcgcgc atcttggtca ctccattagt ttgcatgcac
      721 agactcagaa cagcgctctt gtggggtatg ccgattttgt ggcctcaatt tatttgattt
      781 caaaggacag attatccaag acagtgtctc cggaagcggc tcattacaag gcattgttta
      841 tttgtgccag ttcccagtgg cagggcgaac agacggaacc agccagggac aatctgcaac
      901 gcatcaagga agacctcaat aaactggatg cctcaacttt gacacgcgag atcagggacg
      961 tctttgccca aatgttagca ctcttgccgg ccacaatgaa acaggatgaa cgcgcgcagg
     1021 tgctgatccg cgatattgag tggaagttga aaaacacgtg aagagcgggg tctgcccagt
     1081 ggggatcaat tggtcaactt gctggtatcg agtttgacac cgatctcctg caattttttg
     1141 agcatgtcct ctttatcaac actcttgtaa taggcgtccg tcgcgtcgac caggcctttc
     1201 tttaccagct cctgcaggga gccattgagc aacatgttgc caaaggcctt gcccatctgc
     1261 atgagcgatt caatctggtg tgtcttgccg tcacgga
//
LOCUS       DATDUN010000281         1207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_179524, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000281 DATDUN010000000
VERSION     DATDUN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179524"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..258)
                     /locus_tag="VJC18_03860"
     tRNA            complement(186..258)
                     /locus_tag="VJC18_03860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Lys,seq:ttt)
     gene            527..>1207
                     /locus_tag="VJC18_03865"
     CDS             527..>1207
                     /locus_tag="VJC18_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44546.1"
                     /translation="MAFNLEKFFIDVFAPQKDEVVTVMYDLPHENITDKPAWLERRKM
                     AKAWHKIITGFAKRYDLTVNPPLSYLATGANNANIPEFGILNNQKTRLEDVISKSTIV
                     IVMPQYSATAPLFILTKKYKKLRVGSMPGVAKFMEETGLSADYKKIAETCRHLAPHFS
                     KATGVAVEFSTGHHCHFDLSNHNHIGCDNGCLWPRSDSGTEEALHNLPSGEVYTVPNE
                     AVTSKTHGE"
BASE COUNT          352 a          320 c          250 g          285 t
ORIGIN      
        1 agggttatgt cctaggcctc tagacgatag ggacagaact ttttgacaat agtcaaaaag
       61 atagtttttg gtagttggca gatcgtagtt agaggacatc aacaaaaaca aacctgcttc
      121 ccaactacta cctacaaatt accaactact aactactaaa cttttctgcg atgcagaaaa
      181 gtttgtgagg cgcctgggat ttgaacccag gactcccgcc ttaaaagggc ggtgctctac
      241 cgactgagct agcgcctctc ctcactcccc cagtgcagtg cctcccgatc atcgatcctt
      301 cgggggtggc gtctctaact ccttaatatc acaacaagtt acgaccgcca tttaaggacg
      361 ccctcaatac atgaactcag tatttctagt caattgatat ttataaaaat ccctgtccgt
      421 atccaaataa aatgattcac gtattgataa tcatattgca acgaaacagc catacaattt
      481 atacgtcaca ggtcattaac aaccattttg aaatcaggag aatcctatgg cttttaattt
      541 ggaaaaattt tttattgatg tgttcgcacc gcaaaaagac gaggtcgtga ccgtgatgta
      601 cgacctgccc cacgagaaca tcacagacaa acccgcgtgg ctggaacgtc gcaaaatggc
      661 caaggcctgg cacaaaatca ttaccggatt tgccaaacgc tacgacctga ctgttaatcc
      721 ccccttgagt tacctggcca caggggcaaa caacgccaac atccccgagt ttggcatcct
      781 caacaatcaa aaaacaagac ttgaggatgt gatcagcaaa tcgaccatcg tcattgtcat
      841 gccccaatat tcggccacgg cgccactgtt catcctgacc aaaaaatata aaaaactacg
      901 tgtcggcagc atgcctggcg ttgcaaaatt catggaggaa acgggcctgt ccgcggacta
      961 caaaaagata gccgagacct gtcgccactt agcgccgcat ttttccaaag ccaccggcgt
     1021 tgcagtggaa ttttcgaccg gacatcattg tcactttgac ctgtccaatc ataatcacat
     1081 tggttgtgat aacggttgcc tttggccacg ttccgattcc ggaacggagg aggctcttca
     1141 caacctgccc tcaggcgagg tatacactgt acccaacgag gcggtcacca gcaaaaccca
     1201 cggtgag
//
LOCUS       DATDUN010000282         1248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_54671, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000282 DATDUN010000000
VERSION     DATDUN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54671"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1176
                     /locus_tag="VJC18_03870"
     CDS             259..1176
                     /locus_tag="VJC18_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HLD44547.1"
                     /translation="MHKAYTIFQNHIIEAETMEAGSILVFINPDDLEKHLLVDQFKID
                     EHTLHSSLDPDELSRLEFEPNHLAIIYKRPKSYSGKEKLLFRVSSAGLFLFQDKLVIL
                     LSEDIPLFDGKQFSRVSSLPDVLLRLVYRSVFHFLEHLKIINMLTDELESQINESMDN
                     KKLLHLFTLGKSLVYYVNSINSNSLLVEKLKYNATKLGFTPEEIEFVDDLMIENTQCL
                     KQAEIYSNILASLMDARASIVGNNLNVLMKTLNIIIIIIMVPTFVVSAFSMNVPHPLS
                     SNPWAFWHILGLALFSMLLIVLMWRKKKW"
BASE COUNT          346 a          343 c          243 g          316 t
ORIGIN      
        1 tgactggaaa agacgtgaca agttatgaaa tacatacgaa atggtccgcg ctatttcaaa
       61 atagcaacaa acataccatt cagacaaaaa aacataacaa gttatttcaa cagattacaa
      121 aagccaattt cgcgataggt aaaacttgcc tctttcctga cccaaaattg cccaaccccg
      181 aataggaacc cgcctttttt tccgctcaag cctcattctc atattgcccc cattggcaaa
      241 atcgattaga cacgccccgt gcacaaagcg tataccattt ttcagaatca catcatcgag
      301 gcggaaacaa tggaggcagg gtctatcctt gttttcatca atccggatga tctcgaaaaa
      361 cacctgttgg tcgaccagtt caaaatagac gagcacacgc tccattcttc actggacccg
      421 gatgaactgt cgcgcctgga atttgaaccc aatcacctgg ccatcattta caaacgtccc
      481 aaaagttatt cgggcaagga aaagctcctg tttcgcgtgt cctctgccgg tttgttttta
      541 tttcaggata aactcgtcat cctcctttcg gaggacatcc ctctcttcga tggcaaacaa
      601 ttttcgcggg tttcgtcctt gccagatgtg ttgctccgac tggtgtaccg ttccgtgttt
      661 cattttttgg aacacctcaa gatcatcaac atgctgaccg acgaactgga atcgcaaatc
      721 aacgaatcaa tggacaataa aaaactcctg cacctgttca cgttgggcaa gagccttgtc
      781 tattacgtca actccatcaa ctccaactca cttctggttg agaaattaaa atacaacgcc
      841 acgaaactgg gattcacgcc cgaagaaatc gaatttgtag acgacttgat gatcgaaaac
      901 acccagtgtc tcaaacaggc cgagatctat tccaacattc tggccagcct gatggacgcg
      961 cgcgcctcta tcgtgggcaa taatctcaac gtcctcatga agaccctgaa catcatcatc
     1021 atcatcatca tggtccccac ttttgtggtc agcgcctttt cgatgaacgt tccccacccg
     1081 ctaagtagta acccttgggc cttctggcac attctggggt tggccctctt ctccatgctc
     1141 ctcattgtgc tgatgtggcg aaaaaagaag tggtgatcac gcggaattta ggaccacact
     1201 cccgcacaca aagcgccaca gtgagggcac ctgccctgtt gcaattct
//
LOCUS       DATDUN010000283         1299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_220137, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000283 DATDUN010000000
VERSION     DATDUN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220137"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VJC18_03875"
     CDS             complement(<1..412)
                     /locus_tag="VJC18_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44548.1"
                     /translation="MKIDIYKSDEKQAQMQKFLQYGTVMVFVDSRHPEVMVPGYLKGD
                     YQLRLNFDYAYELEDFRVLADRIEASLSFNKKDFFCVLPYEAVYLMVNHSIQRGSLFV
                     ETVPVEMLQFFADEAQAGEGKKSKKQETVAQKKSP"
     gene            571..>1299
                     /gene="xseA"
                     /locus_tag="VJC18_03880"
     CDS             571..>1299
                     /gene="xseA"
                     /locus_tag="VJC18_03880"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HLD44549.1"
                     /translation="MSNNLANDSKCVLSITDLIAQVNDALEDRFQSVWVRGEISNFKA
                     YPSGHYYFTLKDQNAQISAVMFKGYNKALKFEMENGLSVIAHGKLSVYEAQGRFQIQI
                     NHAEPDGLGALQLAFEQLKNKLQQEGLFDPKRKKPLPVLPRRLGVVTSLQGAAIRDII
                     TVAQRRFANLDMVIYPVKVQGEGAAQEIAGAIDYFSREQNCDVLIVGRGGGSLEDLWA
                     FNEEAVARAVARCTIPLVSAVGHET"
BASE COUNT          301 a          322 c          323 g          353 t
ORIGIN      
        1 gagggctctt tttttgcgct acggtctcct gctttttgct ttttttgcct tctccggcct
       61 gggcctcatc ggcaaaaaac tgtaacatct ccacgggcac ggtctcaaca aagagagatc
      121 ctcgttggat actgtgattc accatgaggt acacagcctc gtaaggaagg acacagaaaa
      181 aatctttttt attaaagctc agggaggcct caatgcggtc cgccagcacc ctgaaatcct
      241 caagctcgta ggcataatcg aagttgagcc tgagttggta gtcccctttg agataccctg
      301 gcaccatgac ctcgggatga cgcgaatcga caaagaccat gacggtgccg tactgcagga
      361 acttctgcat ctgggcctgt ttttcgtcgc ttttgtagat gtcgatcttc atgcccttat
      421 attataaccc caagtttttt gttcgtaaat tgttttttgt ttcttttatg acaaggataa
      481 cttgaattcc accagaaatc cttatagttt tgtattggtt ggttcgccag tcacctgtgc
      541 ggaatgtttt cacctgtgtc atattcgatc atgtccaata accttgcaaa cgactcaaaa
      601 tgtgttctct ccatcaccga cctcatcgct caggtcaacg atgctcttga agatcgcttt
      661 cagagtgttt gggtgcgggg tgagatttct aattttaagg cctacccctc gggacattat
      721 tatttcactc tcaaggacca aaacgcacag atcagcgccg tcatgttcaa gggttacaac
      781 aaggccctca aattcgaaat ggaaaatggt ctttcggtga ttgcacacgg caaactctct
      841 gtctatgagg ctcagggccg gtttcagatc cagattaacc atgccgaacc cgatggtttg
      901 ggtgccctgc agttggcctt tgagcaactc aaaaacaaac tacagcaaga agggctgttt
      961 gacccaaaaa gaaaaaagcc cctgcccgtc ctgccccgca gattgggtgt ggtgacttct
     1021 ttgcaaggag ccgccattcg cgatatcatt acggtggcgc agaggcgttt tgccaacctg
     1081 gatatggtga tttatcccgt caaggtgcag ggtgagggcg cggcacagga aatcgccggg
     1141 gccatcgatt atttttcacg tgaacaaaac tgcgatgtgc tcattgtggg gcgcggaggc
     1201 ggttcccttg aagatctgtg ggccttcaat gaagaggccg tggcgagagc tgtggcccgc
     1261 tgtacgatcc cgctcgtgtc cgctgtgggt catgagacc
//
LOCUS       DATDUN010000284         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_429018, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000284 DATDUN010000000
VERSION     DATDUN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429018"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VJC18_03885"
     CDS             <1..414
                     /locus_tag="VJC18_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HLD44550.1"
                     /translation="AKEGINFGYLPGQNRTKVSLSRDRLLFDAKQDVLDMAKTYVPPT
                     PREDILVPGVGGKMAMFSAIEGYLAQGLISAHDALIAKKLAHVMCGGDIPTQARVSEQ
                     YLLDIEREAFISLCGEEKSQARMQSLLMTGKPLRN"
     gene            complement(494..2233)
                     /locus_tag="VJC18_03890"
     CDS             complement(494..2233)
                     /locus_tag="VJC18_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44551.1"
                     /translation="MLAKCATTVPAMIEAVFPALAGPKIPAPVTTAGRPPKRTHFVDT
                     APNTQGIAIRQHPLTQSPVRAAELTTHAITPPSTIATCPANSIPMSALALSGMFLSGC
                     DGVESITQLVFVGLSLMSLGYASGRGYQAISSALQKHNLVGKYWVEKLETEEMKSYAL
                     SRGLAIEALSNVVVAGCLLNIGTEWLFSTHNPLTGLASCLVALGAVYVMRKNNVVRTS
                     PEALEEKRAQIIAELQKGFLNETLSSGLNPRLFAEDPFFRECLRYFFSSTEYGRVPHL
                     NTSPFVSSHLAPLQVYADQQDLFMAFAEFMVIKLRHNWVPIENRRRIAQGLCDYLEHI
                     CPHCPDGLLWRVLPLLSDSDLPMRHSLTRFFELGLARMTPLQRQIHIQKLTTEIKGAT
                     YHDEDNEGSDYLRSLAMVLAASWDVMNDDQRKPLLRKFVRYIDVRRLGRELRMIAEKI
                     SPEDDGISVYSNESLTAYLSAYLGTSDTLPTAKTLRTYCDNIDPTNPNKLTDALVAFE
                     VNTLGLPLQRKQELTSLLQALALTGDPTLQTFCGKAIEILKAKNASASSSQTTTEPSS
                     KARSAQAQAKTTS"
BASE COUNT          544 a          504 c          638 g          598 t
ORIGIN      
        1 gccaaagagg ggatcaattt tgggtacctg ccgggacaaa atcgtaccaa ggtttcactc
       61 tctcgcgatc gtttgttgtt tgatgccaag caggatgtgt tagacatggc caagacctat
      121 gtgccaccca caccgcgtga agacattctg gtaccgggtg ttggtggcaa gatggccatg
      181 ttctcagcga ttgaagggta tttggcgcaa gggctcatct ccgcgcatga cgccttgatt
      241 gccaaaaagc tggcgcatgt gatgtgtggc ggtgatatcc ccacgcaggc ccgtgtgagt
      301 gagcagtatt tactcgacat cgaacgtgag gcgttcatca gtctgtgtgg tgaggaaaaa
      361 tcacaggcac gcatgcagtc actgctcatg acgggcaaac cgttacgaaa ttgattcaaa
      421 gcaaaataga caaattcaaa aggcgcccat tcgggcgacg cgaataggag cactcccatg
      481 cctgggtggt gttttacgag gtggtcttgg cttgtgcttg tgcggaacgc gctttggaac
      541 tgggttctgt tgttgtttgg gagcttgaag ctgacgcgtt ttttgccttg aggatttcaa
      601 tggccttgcc acaaaaggtt tggagtgtgg ggtcgcctgt gagggccaat gcttgcaaca
      661 gggaggtcaa ttcctgtttg cgctgcagtg gaagtcccaa agtattgact tcaaaggcga
      721 ccaaggcgtc tgtgagttta ttgggatttg ttgggtcgat gttgtcgcag tacgtccgca
      781 gtgtcttggc ggtagggagt gtgtcacttg ttcccagata ggcagaaaga taggctgtga
      841 gggactcatt ggaataaaca gaaatgccgt cgtcttcagg ggatatcttt tcagcgatca
      901 ttctgagttc tctgcccagt cggcgaacgt caatatatct tacaaatttg cgcaacagcg
      961 gttttcgttg gtcatcattc atgacgtccc acgaggcggc taggaccatc gccaagcttc
     1021 tgaggtaatc tgatccttca ttatcttcgt catggtaggt ggcccctttt atctcggttg
     1081 tcaatttttg aatatggatt tgtctctgga gaggggtcat tctggccaaa cccaattcaa
     1141 aaaagcgcgt gagggaatgc ctcattggca agtcactgtc actcaacaaa ggcaagacac
     1201 gccataagag cccatctgga caatgcgggc aaatatgttc cagataatca catagtcctt
     1261 gtgcaatgcg gcgccgattt tcaatgggga cccaattgtg tcgaagcttg atgaccatga
     1321 attccgcaaa ggccataaac aggtcttgtt gatcagcgta gacttgtaag ggggcaaggt
     1381 gggaagacac aaaaggcgag gtgtttaagt gagggaccct cccatattcg gtgctcgaaa
     1441 aaaagtatct gaggcactct cgaaaaaagg gatcttctgc gaatagtctg ggattaagcc
     1501 ctgaagaaag tgtttcgttc aaaaaacctt tttgcaattc tgctataatt tgcgcgcgtt
     1561 tttcttccag cgcctctggt gatgtgcgga ctacattgtt tttgcgcatg acatacacgg
     1621 ctcccagcgc tactaagcaa gaggcaagcc ccgtgagcgg gttgtgggtc gagaaaagcc
     1681 attcagttcc gatattgaga agacaacccg caacgacaac gttgctcaag gcctcaatgg
     1741 ccaaaccacg ggatagggca taagacttca tttcttctgt ttccagtttt tcgacccaat
     1801 atttaccgac caggttgtgt ttttgtagtg cgctagaaat cgcttggtag ccccgtccac
     1861 tggcataacc caagctcatg agggataaac caacaaatac aagttgtgtt atggactcaa
     1921 cgccatcaca accagacaag aacatgccgc tcaaagccaa ggcggacatg ggtatcgagt
     1981 tggctggaca tgttgctatc gttgaagggg gagtgattgc gtgtgttgtg agctcagcgg
     2041 ctctcacagg agactgtgtg agaggatgct ggcggatcgc tatgccctgt gtgtttggag
     2101 ccgtgtcaac aaaatgggtt cgttttgggg gacgccctgc tgtcgttact ggggctggaa
     2161 tcttggggcc ggctagggcc ggaaagacgg cctcaatcat agcgggtacc gttgttgcac
     2221 atttggcaag catgacagtt tctccttgtt tgtatggtca tcgtttgatc tctttaaaag
     2281 ttgt
//
LOCUS       DATDUN010000285         3401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_54854, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000285 DATDUN010000000
VERSION     DATDUN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54854"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VJC18_03895"
     CDS             <1..1002
                     /locus_tag="VJC18_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HLD44552.1"
                     /translation="CEDICRVATKEVIIATKSTVPVGTGDELDEIFRKNLKYPFVVFS
                     NPEFLKEGDAVNDFMKPDRVVVGTNNDRIIPLLRELYAPFTHQRNRIIFMSRRSSEIT
                     KYASNCMLALRISFMNELARFCDKTGANINDIRSGMGADPRIGPAFLYPGIGYGGSCF
                     PKDVKALIRAGHKHDLPLKTIMACDEVNQEQAVYFYHKIIHAFGGEKNLKDKKIAVWG
                     LAFKAKTDDIRESQSLKIIDLLLKAGAHMDVSDPQAMDNVKTELLDSLTYHDNCMSCL
                     AGADALFVATDWDDYKSPDFNEMKKVMKTPRIFDGRNLYNPEVVRKHGLEYWGVGVG"
     gene            complement(1072..2526)
                     /gene="cysS"
                     /locus_tag="VJC18_03900"
     CDS             complement(1072..2526)
                     /gene="cysS"
                     /locus_tag="VJC18_03900"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HLD44553.1"
                     /translation="MMLHLYNTLTRKKEEFKPLTDGKVGMYACGVTVYDYCHLGNARA
                     MVNFDVLHRYLKYVGYDVTFVRNFTDVDDKIIRKANAENISCSEVTSKYIAAFYEDTN
                     ALGLVNPTIEPRATDHIPDIIAIINKLVDKGLAYQIGGDVFYAVRSFPQYGKLSGKNI
                     EDLESGARVDVSDVKKDPLDFALWKSAKPNEPKWPSPWGEGRPGWHIECSAMSTKYLG
                     DTFDIHCGGCDLIFPHHENEIAQSEGATGKPFARFWVHNGFLNIDSEKMSKSLGNFFT
                     TREVLKKFPAEAIRHFILSAHYRSPIDYSEKNISDSIKALERFYQTKSRLEKFIQQPS
                     DPAIPSQQHVPAKLENLKNEFIMALDDDLNTAMVMGLVFEWVRTWNKLIDENRANQKD
                     AKDFLDIINDIHKVLGVFGSDANAYLDELKQKGMAGLLVLEEDILKLIEERKLARKNR
                     DFKRSDEIRDQLLKQGITLKDNTDGTTSWMVNKL"
     gene            complement(2682..3161)
                     /gene="ispF"
                     /locus_tag="VJC18_03905"
     CDS             complement(2682..3161)
                     /gene="ispF"
                     /locus_tag="VJC18_03905"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006516237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HLD44554.1"
                     /translation="MNIRIGMGYDVHRLVAGRPCIIGGVNFEHDKGLLGHSDADVLLH
                     AICDALLGALALGDIGKLFPDTDPQYAGAASKKLLKECFLRVKEKGFVIGNLDTVVVC
                     EKPKLQDKRAEMSRVIAAILETSVDNVSIKATTEEKLGFTGREEGIAAWAYVLLTEA"
BASE COUNT          938 a          819 c          775 g          869 t
ORIGIN      
        1 tgtgaagaca tctgcagggt cgccaccaaa gaggtcatca tcgctaccaa gagcactgtg
       61 cccgtgggca ctggcgacga actggatgag attttcagaa aaaacctcaa ataccccttc
      121 gtggtttttt ccaaccccga atttttgaaa gaaggcgacg cggtcaacga tttcatgaaa
      181 ccagatcgcg tggtggtggg caccaacaac gaccgcatta tccctttgct cagggaactg
      241 tacgccccct tcacccacca gcgtaaccgc atcattttca tgagcagacg ctcatcagaa
      301 atcacaaaat acgcctcaaa ttgcatgctc gccctgcgta tttcgttcat gaacgagttg
      361 gcccggttct gcgacaagac cggcgccaac atcaacgaca tccgctcagg gatgggtgcc
      421 gacccgcgca ttggcccggc ttttttgtac ccgggcattg gttacggggg atcgtgtttt
      481 cccaaagatg tcaaagccct cattcgcgcc gggcacaagc acgatctgcc actcaaaaca
      541 atcatggcct gtgatgaggt caatcaggaa caggcagtct acttctatca caagatcatc
      601 catgcgtttg gtggtgaaaa aaatctcaaa gataaaaaaa ttgctgtttg gggcttggct
      661 ttcaaggcca aaacagatga tatccgcgaa tcacaatcgt taaaaatcat tgatctgttg
      721 ctcaaagccg gggcccatat ggatgtaagc gacccacaag cgatggacaa cgtgaaaact
      781 gagcttctcg acagcctgac ttaccacgat aactgcatga gctgtcttgc tggtgctgac
      841 gccctgtttg tggcgaccga ttgggatgat tacaagagtc cggattttaa cgagatgaaa
      901 aaggtcatga aaacaccgcg catctttgat ggcagaaacc tctataatcc ggaagtggtc
      961 agaaaacacg gactggaata ttggggagtg ggggtggggt aaacgaagct atgaaaaaac
     1021 gaaaacacga gggacgaaag gacgaaagga atgttttgtc atcgcgagct tctataactt
     1081 gttcaccatc caactcgtcg tgccatcagt attatccttg agggtgatgc cttgtttcaa
     1141 gagttggtcc cgaatttcat cggatctttt aaagtctctg ttcttgcggg cgagttttcg
     1201 ctcctcaatc aacttcagaa tgtcttcttc caaaaccaac agccccgcca tgcccttctg
     1261 tttgagctcg tcaaggtagg cattggcatc agagccaaag acacccagca ctttatgaat
     1321 atcgttgatg atgtccaaaa aatccttggc atctttttga ttggcccgat tttcatcaat
     1381 gagcttattc caagtcctca cccattcaaa gaccagcccc ataaccatgg ctgtgttgag
     1441 gtcatcgtcc aaggccataa tgaattcatt ttttagattt tcgagtttgg caggaacatg
     1501 ttgttgagag gggatggcgg ggtcggaggg ttgctggata aatttctcta atcgcgattt
     1561 ggtttggtaa aaacgctcca gagccttgat ggaatctgat atgttttttt cactgtaatc
     1621 gatgggagaa cggtaatgcg cactcaatat aaaatgcctg atggcttctg ccggaaattt
     1681 ttttaaaacc tcacgagtcg taaaaaaatt acccagcgac ttgctcatct tctcgctatc
     1741 gatgttgaga aaaccgttat gaacccaaaa ccgcgcaaaa ggtttgcccg tggcgccttc
     1801 ggattgtgcg atttcgtttt cgtgatgcgg aaaaatgagg tcacacccgc cacagtggat
     1861 gtcaaacgtg tcgcccagat atttggtgct catggccgaa cattcgatgt gccagcccgg
     1921 cctgccctca ccccacggcg aaggccattt gggttcattg ggtttggccg atttccaaag
     1981 ggcaaaatcc agcgggtctt ttttgacatc gctcacatcg acccgtgcgc cggattccag
     2041 atcttcgatg ttcttgccac tgagtttacc atactgtgga aaactcctga cagcataaaa
     2101 cacatcgcca ccaatctggt aggccaaacc tttgtccacc agtttattta tgatcgcgat
     2161 gatgtcgggg atatggtcgg tcgccctggg ctctattgta ggattcacaa gccccagcgc
     2221 atttgtgtct tcataaaacg ccgcgatgta ttttgaggtg acctcagagc aagagatatt
     2281 ttcggcattg gccttacgga tgattttatc gtcaacatca gtaaaattgc gcacgaacgt
     2341 cacatcgtaa ccgacgtatt tcagataacg atggaggacg tcaaaattaa ccatggcccg
     2401 cgcattgccc aagtgacaat aatcatagac ggtcacgcca caggcgtaca tgccaacttt
     2461 gccatcggtg agcggtttaa attcttcttt tttacgagtg agggtgttgt agagatgtaa
     2521 cataataacg tgttcgagtg tttacaaaat aacgcttaca gccgataccc tcaggggcgc
     2581 aaggcgcaag caaaacaatt gcttcaattg tctcaacgat caactacatc aattgccaat
     2641 tgtctgttgc tcattgaagt agttgtagca attgatttgc cttatgcctc tgttaataac
     2701 acatacgccc aagcggcgat gccttcctca cggcccgtga acccgagctt ttcttcggtt
     2761 gtggccttga tggacacgtt gtccacgctg gtttctaaaa tagccgctat gacgcgcgac
     2821 atctccgccc ttttgtcttg aagcttgggt ttttcgcaaa ccacaaccgt atcgagattg
     2881 ccaatgacaa aacccttttc cttgacgcgc aaaaaacact ctttaagaag ttttttggaa
     2941 gcggctccgg cgtattgggg atcagtatcg ggaaacaatt tgccgatatc acccaatgcc
     3001 aaagccccaa gaagtgcatc acaaatagcg tgcagaagaa catcggcatc ggaatgccca
     3061 agaagtccct tgtcgtgttc aaagttcaca ccaccaataa tacaggggcg gccagccaca
     3121 aggcgatgca cgtcgtaacc catgccaatc ctgatattca tgccggagaa cataacatca
     3181 agaatgcaac tagggcaata ggattttgtt tgttgaacat aataatgcta atcttttcaa
     3241 tcttttagat catcatcaaa atattcatcc caatcaattg acaatttacc tcgtttggat
     3301 tttagaattt gggtccgatt ccggagaaat gagaatatga aaaaaaacag ccaaaaaaat
     3361 aagcagaaga aaaaaatcgt aaaaaactca gcaagaaaaa a
//
LOCUS       DATDUN010000286         1018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_220447, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000286 DATDUN010000000
VERSION     DATDUN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220447"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VJC18_03910"
     CDS             complement(<1..447)
                     /locus_tag="VJC18_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44555.1"
                     /translation="MFKKLFTTMTVVFFALLIVTLQSQEARAGFLPSNLKYHTLKTEH
                     FSIHFPEGLGPVAKEMQIISEEAYQNIVTRLDWQPWGRTHVVLTDKSDKANGMATVLP
                     YNYILLYITSPDADSNLDHYKDYWRMLFNHEFSHIVHIDKHYRWATP"
     gene            644..>1018
                     /locus_tag="VJC18_03915"
     CDS             644..>1018
                     /locus_tag="VJC18_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015955785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLD44556.1"
                     /translation="MTIENVIIIGSGPAGYMAGIYTARAGLAPLLFEGYQSGGQLMIT
                     TDIENFPGYPGGVAGPEMMEDLKKQALRFGTRLITQDVSRVDLLQQPFCIESEGKEYF
                     AKALIIATGATASYLNIDSEKKF"
BASE COUNT          269 a          205 c          283 g          261 t
ORIGIN      
        1 gggggtagcc caccgatagt gcttgtcgat gtgcacaatg tgtgagaatt cgtgattgaa
       61 aagcatgcgc cagtaatctt tgtagtggtc aaggttggaa tcagcatcgg gagaggtgat
      121 gtacagcagg atgtagttgt aaggaagcac tgtggccatg ccgttggcct tgtctgattt
      181 gtcggtcaac acaacgtgcg tgcggcccca aggttgccag tcaagacggg tcacgatgtt
      241 ttgataggcc tcttcgctga tgatttgcat ctcttttgcc acgggtccca gcccttcggg
      301 aaaatggata ctgaaatgtt ctgttttgag ggtgtggtac ttgaggttgc tggggagaaa
      361 acctgctcgc gcttcttgtg actgtagggt gacgataagc aacgcgaaaa atacaacagt
      421 cattgtagta aaaagttttt tgaacatgaa aaacacctgc ccccctgata gagaatgaca
      481 cgagtcgtcg ccggcagaca cggaatggca acaactaaat acagtccaat ttcatactca
      541 actgttggac ttatgtcgat aaatttatta tggtttacag aggtcgatga ggtacggata
      601 cgggcacaaa tagcgaggca tcatttcaca aaaggagttg ggtatgacca ttgaaaatgt
      661 catcattatc ggttcgggtc cagcggggta tatggctggg atttacacag ccagggcagg
      721 actggcgcca ttgttgtttg aaggctacca atcgggcggg cagctcatga tcaccaccga
      781 catcgaaaat tttccagggt atcccggtgg tgtggcaggt ccagaaatga tggaagatct
      841 taaaaaacag gccttgcggt ttggcacacg tttgatcacg caggatgtga gcagggttga
      901 tttgttacaa cagccatttt gcattgagtc agagggaaag gaatattttg ccaaggccct
      961 gatcattgct accggtgcga ccgccagtta tctgaacatt gattccgaaa aaaaattc
//
LOCUS       DATDUN010000287         3874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_55186, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000287 DATDUN010000000
VERSION     DATDUN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55186"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VJC18_03920"
     CDS             <1..625
                     /locus_tag="VJC18_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44557.1"
                     /translation="ATVLEIFAEQAIQHILPSLQSPERFERYGLPVIDEYFGMFFEDL
                     PAKSEVRALLFVIMSKVHVYRNEMDNAIAAVNAGLEACPDTSIEVRASLNLTLAMIAV
                     RQGNYDCAVTQCEAVRKSLSSNLDRDEFVKVMLQIIDIYRWLFQSSQNSAHQKNGLAH
                     VDEVISALGGFRAYDPTDPQWKMLLNYQRHLKTGKMPQAEGQGVLNE"
     gene            646..2400
                     /locus_tag="VJC18_03925"
     CDS             646..2400
                     /locus_tag="VJC18_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44558.1"
                     /translation="MSSVNNTDQSASSQSADLFQALGCVDKNGDGVIEERKGNKKKSE
                     GDEKYIPNIDLDRDGYITQTEAKVHLLTHHDVPRPASLSSDYLTTFDKLREQELVVLL
                     ATLLSGDVLVKKEILCVLEKLVNSPMSERLKFSMASPLLGVITDENDDVKKSAIFLLI
                     DLHFSCGVPLDQQMVEPCLEYLPQTEQYHEKLKLGMALSDLGRANPSDVLHARIVSCL
                     VATFGGQQWLTRFGGQEDLLFALNHPHIDSQVKTAIVAGLAGTLQTDDSSVREDTYRL
                     LLSLANSDASVEVKMAMGQLIDPIILTFSKLLDCDLEIFPPTGALEALCKANIPAELQ
                     TLILNKLLMALGNDDRNIQICSKHVIIALVCQSTIPDLPEKCVQILLGGLSDPNVVVR
                     RNVLGLLTGLISLSFTSYPFYGWSVMGVGQDNEDMSCYEREDDAIVMSSGAGGLLGWT
                     DFALASAEHLWREGYKKNVKSVIAALSAEEPSIRAASAIVLGFCSRRAFCNQTRKKIV
                     KELFETLYDDNTDVRSAARTALSQYGYDSNTLPPHDYTVPQHSLIGTMVEPLKVALKD
                     GDMEVRKQAAAALLAISK"
     gene            complement(2404..2532)
                     /locus_tag="VJC18_03930"
     CDS             complement(2404..2532)
                     /locus_tag="VJC18_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44559.1"
                     /translation="MKKIMLIAVLGLALIASTAMACPNCPCGANCPCGKTCECVKK"
     gene            complement(2597..2875)
                     /locus_tag="VJC18_03935"
     CDS             complement(2597..2875)
                     /locus_tag="VJC18_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HLD44560.1"
                     /translation="MKKGTNHNNKLVALNRIEGQVRGIQNMITEGRYCIDIINVVSAT
                     IGALRKVEASILQDHLQACVLSAFAEKSDRQIKEKIDEVCTLLNKLHR"
     gene            complement(3184..>3874)
                     /locus_tag="VJC18_03940"
     CDS             complement(3184..>3874)
                     /locus_tag="VJC18_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD44561.1"
                     /translation="IQKIFLADGDALVLSNNKLLPILKELSHYFGQGIRISAYATPQN
                     ILYKSHDELCALKNAGLQMIYYGVESGDNLVLKKICKGASAEQIAEGILRAKKAGITV
                     STTNLLGIAGKQYSKQHTQSTAKLLSETSPEFISFLMVMFPLGENRFRTCFGNDYDPL
                     SPLELIQELRETLSDLHVHNSQIRANHASNYLPIKARFPTDKLKTLQLIDEVLNNPDT
                     HKIKPEFLRGL"
BASE COUNT         1011 a          787 c          954 g         1122 t
ORIGIN      
        1 cgctaccgtc ttggaaattt ttgctgagca ggctattcaa cacattctgc cttcactgca
       61 gagcccagag agatttgaac ggtatggact gcctgtcatc gatgaatatt ttggaatgtt
      121 ttttgaagac ctaccggcca aatcagaggt cagggccttg ctgtttgtta tcatgtccaa
      181 agtccatgtt tatcgaaacg agatggacaa tgccattgcg gcggtcaatg cgggacttga
      241 ggcttgtccc gatacaagca ttgaggtgcg agcgtcatta aatctcaccc tggccatgat
      301 cgctgtgcgg cagggcaact atgactgtgc ggtaacccag tgcgaggcag tgcgcaaaag
      361 tctcagctca aatcttgata gagatgagtt tgtgaaggtc atgctacaaa tcattgacat
      421 ctatcgttgg ctgtttcaat ccagtcagaa ttctgcgcat cagaaaaatg gtttggcaca
      481 tgtggatgag gtcattagtg ccttgggtgg ttttcgggcg tatgacccca ctgacccgca
      541 gtggaagatg ctccttaatt atcagaggca tcttaaaaca gggaaaatgc cacaggccga
      601 aggacaaggg gttctgaatg aatagaaccc agtcaggagg aaacgatgtc atcagtaaat
      661 aatacggatc aatctgcttc atcacaatcg gcagatctat ttcaggccct gggttgcgtc
      721 gacaagaatg gagacggggt catagaagaa agaaagggaa acaaaaaaaa atcagaggga
      781 gacgaaaaat acattcccaa tattgatctt gaccgcgatg gctacatcac ccagaccgaa
      841 gcaaaggttc atttattgac gcatcatgat gtccctcgac ccgcttcttt atcgtctgat
      901 tatctgacca cattcgataa attgcgtgaa caggagttgg ttgttctcct tgctacgttg
      961 ttgtccggtg atgtgctggt caaaaaagag atcctgtgtg tcctggaaaa attggtcaac
     1021 tcaccaatga gcgagcgcct gaaattcagt atggcgtctc ctttgttggg tgtcatcaca
     1081 gatgaaaatg atgacgtcaa aaaatctgcc atttttttgt tgattgattt gcattttagt
     1141 tgcggtgtgc ctttggatca gcaaatggtt gagccttgtc ttgaatattt gccacagact
     1201 gagcaatatc atgaaaaatt gaaactgggt atggccttgt cggatctggg cagagcaaat
     1261 ccatccgatg tgcttcatgc gagaatagtg agttgtcttg tagctacctt tggggggcaa
     1321 caatggttga ccagatttgg tggtcaagaa gatcttttgt ttgcattgaa ccatccccac
     1381 attgactcgc aagtcaaaac cgcaattgtt gctggccttg caggcacttt acagaccgat
     1441 gactccagcg ttcgtgagga tacatacaga ctgttattgt ctttggccaa ctcggatgct
     1501 tccgttgaag tgaaaatggc catgggtcaa ttgatcgatc ccatcatcct cacgttctca
     1561 aaactactgg actgtgatct cgaaatattt ccacccacgg gagctcttga ggccttgtgc
     1621 aaggcaaata ttcccgcaga attacaaacc ctcatcctga ataaattgtt gatggctttg
     1681 gggaatgacg acaggaatat tcaaatatgt tcaaaacatg tgatcatcgc actggtgtgt
     1741 cagtccacta tccctgattt gcctgaaaag tgtgttcaaa ttcttctggg tggattgagt
     1801 gatcctaacg tagtggtgcg acgaaatgtc ctagggttac tgacaggcct gattagtttg
     1861 agttttacat cttatccctt ttatggatgg tctgttatgg gagttgggca ggacaatgag
     1921 gatatgagtt gttatgaacg tgaagatgat gccattgtca tgagctcggg ggcaggtggt
     1981 ttgttaggat ggacagattt tgcattagca tctgcggaac atttatggcg tgaagggtac
     2041 aaaaagaatg tgaagagcgt cattgccgca ctctctgctg aagaaccttc aattcgtgcc
     2101 gcctcggcca tcgttttggg cttttgcagt cgacgggctt tttgtaatca gaccagaaaa
     2161 aaaattgtaa aagaactttt tgaaacttta tatgatgaca acacagatgt gagaagtgcg
     2221 gctaggacgg ctttgagtca atatggttat gacagcaaca cattaccgcc ccatgattat
     2281 actgttccac agcattcatt aataggcact atggtagagc ctctcaaagt ggctctgaag
     2341 gatggtgata tggaagtgag aaagcaggcg gccgccgctt tattggctat cagtaaatga
     2401 tagtcacttt ttcacacact cacatgtttt gccacaaggg cagttggcac cacaggggca
     2461 gttgggacag gccatggctg tggaggcaat gagagcgagt cctaaaacgg caatcagcat
     2521 tatttttttc ataaatatcc tttcgggatc atctgcaaca gtcttgtgct ccttggttta
     2581 tgggagtcca gaactgtcat cgatgcagtt tgtttaacaa ggtacagact tcatcaatct
     2641 tctctttgat ttgtcgatct gatttttccg caaaggcaga aagcacgcag gcctggaggt
     2701 ggtcttgcag aatggaggcc tccactttcc tgagggcgcc tatggtagcg ctgaccacgt
     2761 tgatgatatc gatacaataa cggccctcag tgatcatgtt ttgaatgccg cgcacctgac
     2821 cctcaatgcg gttgagagcc accaatttat tgttatgatt tgttcctttt ttcataataa
     2881 tcctttctct ctggttctag catagcatac ccctatgggg tatacaagag ttattttgtc
     2941 ttgttcgtaa tgtcatgaaa tgtccaacga atcctcctcc tcaattgttc tcaacagaga
     3001 atgaaatttg tttacagggc acaacaagtt gtctgctgtt accgataaag ttcatgaaag
     3061 gagttggtgc cgaagtaata agtgatgcgc aggattttat tcgttttgtt ttttactcgg
     3121 cgtaactcat gattcctgtc gctggcaagc aggctgacgt tttataagcc atatcatttt
     3181 ttttcacaaa cctctcaaaa actccggctt gattttgtgt gtgtctgggt tgttcaaaac
     3241 ctcatcgatg agttgtaagg ttttcaattt gtcagtagga aaccgcgcct tgatgggcag
     3301 gtagttggag gcatgattgg ccctgatttg tgaattgtga acatggagat cggagagcgt
     3361 ctcacgcagt tcctggatga gttcaagagg cgatagcggg tcatagtcgt tgccaaaaca
     3421 tgttctgaaa cgattttctc caagaggaaa catcaccatc aaaaaagaaa taaactcggg
     3481 gctcgtttcg ctgaggagtt tggccgtgct ttgtgtgtgt tgttttgaat attgtttgcc
     3541 ggcgattccc aaaagatttg tggtggagac ggttattccg gcctttttgg ctctgaggat
     3601 tccctcggca atttgttctg ctgaggcccc tttgcaaatt tttttaagaa cgaggttatc
     3661 accggattca acgccgtaat aaatcatctg caaaccggca tttttcaggg cacacaattc
     3721 atcatgagat ttgtagagaa tgttttgcgg ggtggcgtag gcactgatgc gaataccctg
     3781 tccaaaatag tgagacagtt ctttcaaaat gggcaggagt ttattgttgg acaacaccaa
     3841 tgcgtcaccg tcagctaaaa agattttttg aatt
//
LOCUS       DATDUN010000288         3739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_220472, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000288 DATDUN010000000
VERSION     DATDUN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220472"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VJC18_03945"
     CDS             <1..943
                     /locus_tag="VJC18_03945"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HLD44562.1"
                     /translation="HFLLQYYTDQKFIPSQILVENVYAEWGGLEEVLAQRAGHKVVIK
                     RPQKGVKKELVTLAHTNLKSHIEQDDQSEKNRQALLLRLKDTFILTRVPYRIECYDVS
                     NLSGSDAVASMVVFSDGEQNEAGYRRFKIRTVKGPNDFAMLREVFVRRFQNTKGDWLK
                     PDLTLVDGGKGQLKQVLSVFAELNVTGTDVVAIAKGRGEGVRAKGSWEKKKEEEFFIP
                     GRKNPIVFNKGATELLFLQRVRDEAHRFAIEYHRKLRDKTLVHSALDDVPGIGPKLKA
                     RLIKAFGSPEAVLRASAQDLRAVRGVTEMLAKTIKRL"
     gene            1110..1763
                     /locus_tag="VJC18_03950"
     CDS             1110..1763
                     /locus_tag="VJC18_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLD44563.1"
                     /translation="MPRINRFDYLANDMLLHVRIQINNGEFRFFEERHFELFNRICRM
                     YLKKYPTIKLTDYEWMSNHAHLIFHIDIYTDLPKFMHDFCWRFAFEYNKMQNRKGHLF
                     QQRYRASNVNTDQYALSCQRYVYRNQLRAGMVKELQDTKWSSYHYYAYGQPNGLITPI
                     FTYEQFGNTPEKQQIEFRFFVEKMPEHEETLWRAKLLHPLLKTKKEIVLNYLKKCHV"
     gene            complement(2069..3019)
                     /locus_tag="VJC18_03955"
     CDS             complement(2069..3019)
                     /locus_tag="VJC18_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44564.1"
                     /translation="MFIPHSFANLGMPGSVQFGGDDAMASAAFGAAHPSNQFGEDWIR
                     PYPSWIDRRSHVLTANINGLKLGWPKDPEDHVIPATRDLVMGLFDTKRSRNPHLDIFV
                     FHLPPWGSSLRKLNEPILKNNTSINGITYAGGKAVQFDTGGHFYRGCLAQNTEVTLPG
                     GTEVTAAKNTWLRFSKTYEFYERKKSTQPQYICEVTLAQQATIDGLSLAKGTVVGLDA
                     KGHITSLILADTMRVPVTHHVGARGVIKFLELSADSEITYLDQPPKTVVKSALAVSYE
                     GHRLAGKTRMLRSGAETWLTFAQEMLINRVTGAVITVQRP"
     gene            3256..>3739
                     /locus_tag="VJC18_03960"
     CDS             3256..>3739
                     /locus_tag="VJC18_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44565.1"
                     /translation="MASTQLYISSVDDLHYSGACPARAVACLGHSIFKIIFCRVFLIA
                     SNIDERVAEREVTFMNTTKNIFLNMLIIAVIALVTSCGSMDQTVIDAGLENESVIAND
                     AEPSALITGSFAEADVGNNLIGFSVAFQSRSGSFGDADAFACGDFNGDGVQDYAFSDA
                     A"
BASE COUNT         1004 a          781 c          875 g         1079 t
ORIGIN      
        1 acactttctt ttgcagtatt acaccgatca aaaattcatt ccttcgcaga ttctggttga
       61 aaatgtgtac gctgaatggg gtggccttga ggaagtgtta gcccaaaggg cagggcataa
      121 ggtggtgatc aaacgccctc aaaaaggggt caaaaaggaa ttggtgacgc tggcgcacac
      181 gaatctcaag agtcatatcg aacaggacga ccagagtgag aaaaaccgac aagctctctt
      241 gttgcgtctc aaggacacgt tcatactcac tcgtgtgcct taccgcatag aatgttacga
      301 tgtttcgaat ctgtcgggga gtgatgctgt agcctcgatg gtcgtgtttt ctgatggaga
      361 acagaatgag gcgggttatc gtcgtttcaa aatcagaacg gttaagggtc ccaacgattt
      421 tgcgatgctc agggaggtct ttgtccgtcg ttttcaaaat acaaaaggtg attggttaaa
      481 gcccgatctg actctggtcg atggcggcaa gggtcaactc aaacaggtct tgtccgtatt
      541 tgcggagttg aatgtaactg gtaccgacgt tgtggcgatc gccaaggggc ggggggaggg
      601 cgtgcgcgcg aaggggagtt gggaaaaaaa gaaggaggaa gaatttttta ttccggggcg
      661 caaaaatccc attgtgttca ataagggggc aacggaatta ttgtttttac agcgtgtgcg
      721 tgacgaggct catcggtttg ccattgagta tcatcgcaaa ttgcgtgata aaaccttggt
      781 tcattctgcg ttggatgatg ttcccggcat tggccccaag ctcaaggctc gtctcatcaa
      841 ggcctttggt tcccccgagg ctgttttgcg tgcgagcgca caagatctac gtgcagtcag
      901 gggggtgacg gagatgttgg ccaagaccat taaacgcctg taaaggtgcc acatactttg
      961 tctcatatac gcttgttagt gtcccatttt tgtgtctcaa tactgagaat ggcacatttt
     1021 ctttcgggtc tattttttct atctgactgt aatttcaaca taatatattt tttaattttg
     1081 gcatcttctt tgcttatatt ttttgtatta tgcccagaat caatcgtttt gactatttgg
     1141 ccaatgacat gctcttgcat gtcagaatac aaatcaacaa tggtgagttc cgattctttg
     1201 aagaaagaca ttttgagcta ttcaatagaa tctgtcggat gtatttgaag aaatatccca
     1261 ctatcaaact cactgactac gaatggatga gtaatcacgc tcatttgatt tttcatatcg
     1321 atatctatac tgatcttccc aaattcatgc atgatttctg ttggcgtttt gcatttgaat
     1381 ataacaagat gcaaaatcga aaaggtcatt tgtttcaaca gcgttatcga gcaagcaatg
     1441 tgaacaccga tcaatatgcc ctgagttgcc aacgatatgt ttaccgtaat caattgaggg
     1501 caggaatggt caaggagctc caagatacaa aatggagcag ttatcactat tatgcttatg
     1561 ggcaaccgaa tggcttgatc acccccattt ttacctatga acagttcggc aataccccag
     1621 aaaaacaaca gattgaattt agattctttg tagagaaaat gcctgaacac gaagaaaccc
     1681 tgtggcgtgc aaaactatta cacccccttc ttaaaacaaa aaaagagatt gtcttgaact
     1741 acctgaaaaa atgtcatgtg tgaaaatgtg ataaacaaat aatgccagct ttgttctgat
     1801 ccccaaatgc cctttcttta caagctgcac ttaaaaaaaa tggccgtcaa taataatcct
     1861 tcgagagaat aattacgaat taggtgttgc ataaacaata gacactatat taccaatatt
     1921 acttttaaat aataaaggta tgtggtactt ttttgctgtt gcgtggcaaa aagtatgtgg
     1981 cacctttctt tcatgaaaat gatgttttgg tcacagttgt tgcaataagg tatgtggtac
     2041 ctttcatgca cgatgtgtag gcttacagct aaggtcgttg aacagtgatc acagcacctg
     2101 ttacgcggtt aatgagcatt tcttgagcaa atgtgagcca ggtttccgcg cccgaacgaa
     2161 gcattcttgt tttaccagcc agacgatgac cctcatacga aactgctaac gcactcttaa
     2221 caacggtctt gggcggctgg tcaagatagg tgatctcgct atcggcagaa agttccagaa
     2281 acttgatgac gcctcttgca ccgacgtggt gcgtgacggg gactctcatt gtgtcagcaa
     2341 gtatgagtga ggtaatatgc cctttggcat ccaaaccaac aaccgtgccc tttgcgaggg
     2401 atagtccatc gatagtagcc tgttgtgcca gagtgacttc gcagatatac tgtggttgtg
     2461 ttgatttttt gcgttcataa aattcatacg ttttggaaaa tctgagccaa gtattcttag
     2521 cggcggtgac ctcggtacct cccggtagag tcacttctgt gttttgagca agacaaccac
     2581 gataaaaatg gccgcccgta tcaaactgaa cagcttttcc acccgcatag gtaatgccat
     2641 tgatgcttgt attgtttttg aggatgggtt cattaagttt gcgaagagat gaaccccagg
     2701 ggggtaaatg aaatacaaaa atgtccagat gaggattcct tgatcgtttt gtatcgaata
     2761 gccccatcac caagtctctt gtggcaggaa taacatgatc ttcaggatct tttggccagc
     2821 ccagtttcaa accattgata ttggctgtta gcacatgaga gcgtcgatct atccatgaag
     2881 gataaggacg gatccagtct tcaccgaatt ggttgctggg atgagcggca ccaaatgcgg
     2941 cactcgccat agcatcgtcg ccaccaaact gaactgaacc gggcattccc aagtttgcaa
     3001 aactatgtgg aataaacatt aatacctcct gcggtagacc attgattgaa cgaacccact
     3061 tcgtgtgagc ttttgaggct cgcgtatact cagtgcggct catccagata tctcctatta
     3121 tcgggttctt ttcttaaacg ttgcgtgttt ttgataaggt atgtggcact atttgcaaaa
     3181 gtagtgcatt catttataga atccgcccca ttttgactcc caaaaatgtc ccgtccacaa
     3241 ttgtggactg aatttttggc ttccacacaa ctttacattt catctgtcga tgacctgcac
     3301 tatagcgggg cgtgccctgc acgggctgtt gcctgtttgg gtcattcaat cttcaaaatc
     3361 attttttgcc gcgtcttctt gatcgcatca aatatcgatg aacgcgtggc agagagagag
     3421 gtcactttta tgaacacaac aaaaaatatt tttttgaata tgctgatcat cgctgtgata
     3481 gcgttggtca caagttgcgg ctcaatggat caaaccgtga ttgatgctgg actcgaaaat
     3541 gaatcggtca tcgcaaacga tgccgagccc agcgcactca tcacaggatc ttttgccgag
     3601 gccgatgtgg ggaacaacct cataggtttt tctgtggcct ttcagagtcg ttcaggcagt
     3661 tttggcgatg cagacgcgtt tgcttgcggc gatttcaatg gagatggtgt tcaggattac
     3721 gccttttcgg atgctgctt
//
LOCUS       DATDUN010000289         1119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_220523, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000289 DATDUN010000000
VERSION     DATDUN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220523"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VJC18_03965"
     CDS             complement(<1..311)
                     /locus_tag="VJC18_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019457646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HLD44566.1"
                     /translation="MTTYKVDERDVHFNLFEYLHLGDLTQYDRFKDYNEEIFKMVVTE
                     ALKFAKNEIDPLYVSGDREGCQINQGVVTTPKGFKEAYQKMGENGFIGIDTPVTHGGQ
                     G"
     gene            522..>1119
                     /locus_tag="VJC18_03970"
     CDS             522..>1119
                     /locus_tag="VJC18_03970"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLD44567.1"
                     /translation="MLLTIDIGNTNIVLGFFVHDKFLSEMRLATIKNLPAKQWPEVLL
                     PKFKEQGIDNQTIKHVVIGSVVPELRDNIQNLCHSLLPQSQPLWVTQEHCRKLININV
                     PEPHKVGIDRLINVCAAQHVCHENLLVIDLGTATKFDCVLEGTFIGGAIAPGLLSSAN
                     HLFQNASQLHRIEIKKPPTVIGNNTQACLESGLFFGYLG"
BASE COUNT          298 a          312 c          239 g          270 t
ORIGIN      
        1 aaaccttgcc caccatgtgt gaccggtgtg tcgataccaa tgaacccatt ctcgcccatt
       61 ttttggtagg cttccttaaa gccttttggt gtggtcacca cgccttgatt gatctggcaa
      121 ccctcccgat cacccgacac atacaaagga tcaatttcgt ttttggcaaa cttgagggcc
      181 tcggtcacca ccatcttgaa aatctcttcg ttgtaatcct tgaacctgtc atactgagtc
      241 agatcaccca gatggaggta ctcaaaaagg ttaaaatgca catctctttc atcgactttg
      301 tatgttgtca taaaatatcc tttcttgttt acaaaaatag cctctgcggg gtagcataac
      361 gcacaatgtc actcaagtaa aaacgaagcg actaaaaacg aagcgacgag ggggcgaaaa
      421 cacgatggac gaaacaggtc atcgcctccg gctaacgatc agattctgtc tgtagccgta
      481 agccttgcgc cttgtgcctt acacccaagt ttgataatca catgttatta accatcgaca
      541 tcggcaacac caacatcgtt ctgggatttt tcgtacatga caagtttttg tccgaaatgc
      601 gattggcaac catcaagaac ctgccagcaa agcagtggcc cgaggtattg cttcccaaat
      661 tcaaggagca gggcatcgac aatcaaacca tcaagcatgt ggtgattggc tcagtggtcc
      721 ccgaacttcg agacaacatt caaaacctgt gtcacagcct cctcccccaa agccagcccc
      781 tctgggtcac acaggagcac tgccgcaaac tcattaacat caacgtgcct gaacctcaca
      841 aggtgggcat cgaccggttg atcaatgtct gtgcggcaca gcatgtttgt cacgaaaatc
      901 ttcttgtgat cgatctgggc acagccacca aattcgactg tgtgctcgaa ggcaccttca
      961 tcggcggcgc catcgccccg ggactgctgt cttcggcgaa ccacctgttc cagaatgcct
     1021 cacagttgca ccgcatcgag atcaaaaaac caccaacagt cattggtaac aacacacagg
     1081 cctgtctgga atcgggcctg ttctttggat acctcggtc
//
LOCUS       DATDUN010000290         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_220573, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000290 DATDUN010000000
VERSION     DATDUN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220573"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /gene="atpH"
                     /locus_tag="VJC18_03975"
     CDS             complement(<1..475)
                     /gene="atpH"
                     /locus_tag="VJC18_03975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HLD44568.1"
                     /translation="MKESLIAKRYARALISLAIQANKTDLVGQQLHSTRQLLQDNPSL
                     WAGLTNSDFDSQRRQNIFREIARRLQLDELVQKTIVFMMQKNRLVLMSELCEVYETLA
                     NQEMSRLVMLVTSAVELPKEQYDDITLYFGGQTKKNIILKKHIDTDVLGGVSVKIG"
     gene            complement(472..1029)
                     /locus_tag="VJC18_03980"
     CDS             complement(472..1029)
                     /locus_tag="VJC18_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44569.1"
                     /translation="MTKKRIISLAFILYLALAIVNWLWWGGVPIVYALLNFSLFCTLL
                     ILGTKDRVTTFFCERSDVLSHTMTTAQKTFENASRSFSEAQNNLKLLSEQKQAILDTV
                     VFSAKKEALLMSEETQQLRAQLLSDAKQRAHQEEQKTKRRLKMKTINEATLLARQKII
                     QTLTKKDHEQLGREFLIRIRQETLL"
     gene            complement(1026..1481)
                     /locus_tag="VJC18_03985"
     CDS             complement(1026..1481)
                     /locus_tag="VJC18_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44570.1"
                     /translation="MHILPDFNNIKELALSVSCMWVIFILSLLSLNFLVFKPTLSIIK
                     ERGRRTDGLEKSSRDLCTRTQLEQDRKQTLNEEIMTLGKKLREDILAKALIEKTSLLT
                     KTRQETLAYLDANRTELTQQTQAAEVVLSNNATSLADDICRKLIQKNAA"
     gene            complement(1627..2310)
                     /locus_tag="VJC18_03990"
     CDS             complement(1627..2310)
                     /locus_tag="VJC18_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44571.1"
                     /translation="MITFQPTDTELLDYRLGRTDPSLTRQIEAYLNTHSETKRQLAEH
                     NEIEQLFKNYPLIEPGQSVLERVRHQAARQVRPKFWTTFFDRFALPIPRALAWTATIV
                     LVVGLGYFVMQSNTLTPTSPPYQNAPATLTGNTAPPPSTVVNESKVATDGPATESLAE
                     ADYAQALAFFNDNRFAEACQLFQKIDNTYPDFGKRAELLSYWIIALEKTNQPDLAQKK
                     QAERELISQ"
     gene            complement(2345..2959)
                     /locus_tag="VJC18_03995"
     CDS             complement(2345..2959)
                     /locus_tag="VJC18_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLD44572.1"
                     /translation="MQLTAKDYPHLTNEVLFNRYVGGDLSAFDELLNRHKGLLYTLIL
                     RYVPNRSEADEIFQDVFFKVCKNKELFKESVSFKSWLVTICKNTCIDHTRKRKNRLLN
                     DSFDNLPGDEHRAPSESIASKDLGPDQTLTIQTEGQELEALLDKLPTEQRETFYLKVI
                     MEFTFEEIGESMGCSCNTAKSRFRYALDSLRGLVRRKKVFEKAV"
BASE COUNT          709 a          689 c          825 g          912 t
ORIGIN      
        1 cgcctatttt tacactgacg ccccccagca catcggtgtc gatgtgcttt tttaagatga
       61 tgtttttttt ggtctgccca ccaaaataaa gagtgatgtc gtcgtattgt tctttgggca
      121 attccacggc ggaggtgacc aacatgacca gccgtgacat ttcctgattg gccaaggtct
      181 cgtagacctc acagagctcg ctcatcaaca cgagtcggtt tttttgcatc atgaagacga
      241 tggttttttg aaccaactca tcaagctgaa gacgacgggc gatttcccta aaaatatttt
      301 gccgacgttg agagtcgaaa tccgagttgg tcagccctgc ccacaacgag ggattgtcct
      361 gcaggagttg ccgggtgctg tgcagttgtt gccccacgag atctgttttg tttgcctgaa
      421 tggccagaga gatcagggcg cgggcgtagc gtttggcgat gagggattct ttcacaataa
      481 ggtctcctgc ctgatcctga tcaaaaattc ccggccaagt tgttcgtgat ctttttttgt
      541 gagggtttga atgattttct ggcgggccag cagtgtggct tcgttgattg ttttcatctt
      601 caagcgacgt tttgtttttt gttcttcttg atgtgcgcgc tgtttggcat cggatagcaa
      661 ttgtgcccgc agttgttgcg tttcttccga catcaggagg gcttcttttt ttgccgagaa
      721 caccaccgtg tccagaatgg cctgcttttg ttcgctgagg agcttgaggt tgttttgggc
      781 ctctgagaaa gagcgtgagg cgttctcgaa tgttttttgg gccgtggtca tggtgtgtga
      841 caggacgtcg gagcgctcgc aaaaaaaagt tgtcacacga tctttggtgc ccaaaatcag
      901 cagagtacag aagagcgaga aattaaggag ggcgtaaacg atgggaacac caccccacca
      961 caaccagttc acgatggcca gagcgaggta caaaataaaa gcgagagaga tgattctttt
     1021 tttggtcatg cggcgttctt ttgaatgagt ttacgacaaa tatcgtcggc cagggaggtg
     1081 gcgttgttcg atagcacaac ctcagccgcc tgtgtttgtt gggtcagctc tgttctgttg
     1141 gcatcaaggt acgcgagtgt ttcctgacgt gttttggtca gcaggctggt tttttcgatg
     1201 agggccttgg ccaggatgtc ttcccgcaac ttttttccca gggtcatgat ctcttcgttg
     1261 agagtctgct tgcggtcttg ttcaagctgg gttcttgtgc acagatcgcg tgagcttttt
     1321 tcgaggccgt cggtgcggcg tccccgctct ttaatgatgc tgagtgtggg tttgaacacc
     1381 agaaagttca gtgagagcaa actcaggata aaaatgaccc acatgcagga gacagacagg
     1441 gccaactctt taatattgtt gaagtcggga agtatgtgca tgggctagga acgtaacatc
     1501 gaaacaaaaa agctgtcaag gagatcaggg aggacaatca atcaatcaat caatcaatca
     1561 atcaatcaat caatcaattc atttttatat tacttcattg cttcattggt tgattgtttc
     1621 atttttttat tgtgatatca gttcccgttc cgcttgtttt ttctgtgcga ggtcaggctg
     1681 attggttttt tcaagcgcga tgatccaata ggaaagaagt tctgcacgtt tgccaaaatc
     1741 gggataggtg ttgtcgattt tttgaaacaa ctggcaagcc tctgcgaaac ggttgtcgtt
     1801 aaagaaagcc aaggcctgtg cgtaatcggc ctcggccaga gattctgttg cagggccatc
     1861 agtagccacc tttgattcat tcaccactgt tgagggaggg ggtgctgtgt tgccagtcaa
     1921 tgttgctggc gcgttctgat aaggtggtga ggttggggtc agagtatttg attgcataac
     1981 aaaatagccc agacctacca ccaaaacgat cgtcgcggtc caggccaagg cgcgcggaat
     2041 gggaagggca aagcgatcaa aaaaagttgt ccagaattta ggcctcacct gcctagcggc
     2101 ctgatgcctg acacgttcca gaacagactg accgggttcg atcaggggat aatttttgaa
     2161 caattgctcg atttcgttgt gctcagcgag ctgtcttttt gtttcgctat gtgtgttgag
     2221 gtaggcctct atttgtcgcg tgagggacgg gtcggtcctt cccaggcggt aatcgagtag
     2281 ttctgtgtct gttggttgaa atgttatcat atttgtttaa tctaaattgc agttgctatc
     2341 taggttaaac cgctttttca aaaaccttct tgcgtcgtac caagcctctc aaagagtcaa
     2401 gcgcataccg gaaacgtgat tttgccgtgt tgcaggaaca gcccatggac tcgccgatct
     2461 cttcgaaagt gaattccatg atgaccttca gatagaacgt ctctcgctgt tccgtaggca
     2521 atttatccag cagggcctca agttcctgac cctcggtctg gatcgtcagt gtttgatcag
     2581 gacccagatc cttgctggca attgactcgg acggagcgcg gtgttcgtct ccgggcaaat
     2641 tgtcaaaaga gtcattcaac aacctgtttt ttctctttct tgtgtggtca atacaggtgt
     2701 ttttacaaat ggtgaccaac cacgatttaa acgaaacaga ttccttgaaa agttctttat
     2761 ttttacagac tttgaaaaaa acatcctgaa agatttcgtc ggcttcggag cgattgggca
     2821 cgtacctcag gatgagggtg tagagcagtc ctttgtgtcg gtttagcaac tcgtcaaagg
     2881 ctgataaatc accgcccacg taacggttga acagaacctc gtttgtgagg tgagggtagt
     2941 cttttgcagt cagttgcata gcgctgtgtc ttattgtgag ccaacagtaa ttaccgtgtt
     3001 gtggttctca gagcctattc gaaaactcca tcgagaggca ctttttcggg tcctgccacg
     3061 gctcgctgtt ttttggcgct ctgggctcgc aagctcgccc agcggcgtgc tcgcctattc
     3121 gcgcaaaaac agctc
//
LOCUS       DATDUN010000291         3389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_304599, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000291 DATDUN010000000
VERSION     DATDUN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304599"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(368..2155)
                     /gene="aspS"
                     /locus_tag="VJC18_04000"
     CDS             complement(368..2155)
                     /gene="aspS"
                     /locus_tag="VJC18_04000"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HLD44573.1"
                     /translation="MSEFLLTNQRTHYCHELSTEDVGREVVLFGWVDTRRDHGGLIFV
                     DLRDRFGLTQIVFHPEVDPRVHELGHHLRTEYCLGIKGKVNLRPEGMTNLNLKTGEVE
                     VDVLDFEVFNRSKTPPFMIVDDIDVHEEIRLKYRYLDLRRNKIKNNIIIRSQVCHVVR
                     NYFNEQHFVEVETPCLTKSTPEGARDYLVPSRVQKGSFYALPQSPQIFKQLLMVSGLD
                     RYFQIVKCFRDEDLRADRQPEFTQIDLELSFITQDELFKIMEGLIAKIWYEVKGIAPH
                     TPFARITYDECLERFGLDAPDMRFGMELKTITDVFKVSQFKVFHDIANAGGDSVIKAI
                     NLKNAGDMPRSQIDALEKIVKVYGAKGLAYIRVTETEWASPIAKFFSDAEKQKLTDLF
                     NIEPGDVMLFGAGKKKIVNDSLGNLREHLGEKRGLIDNNKDAFVWVVDFPMFDYDEAA
                     KRHVAIHHPFTSPKTEDMPLLDSEPLKCRANAYDLVLNGNEIGGGSIRIHDQVIQSKV
                     FGLLGIDEERAKQKFGFLLDALQYGAPPHGGIAFGLDRIMMLLTGCTNIRDVIAFPKT
                     QKASDLMSDCPSEVDANQLLELGLRVIES"
     gene            complement(2159..3172)
                     /locus_tag="VJC18_04005"
     CDS             complement(2159..3172)
                     /locus_tag="VJC18_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HLD44574.1"
                     /translation="MEFSKTPQRSQNQDALKGILFPVQQELLALEEAIFGLLDSQIPF
                     VRTVGEHILKNGGKRIRPILTIVCAKMTGFQGNRAAQVGACVEFLHTASLLHDDVIDN
                     AKVRRGKTSANALWGNHVSVLVGDFFYSRASQLLIEQGNTRILKVLADCTIATTEGEI
                     WETLKSSDLDTNRDDYINIIKLKTAFLFQAACQVGGILGGVSDCFVKALSDYGLHLGM
                     AFQLVDDILDYNASEDVFGKSQGIDLREGKLTLPLILSLGRAAPDEKQVIKNAVLADK
                     IDQELFERVRQIIRKYHGFEDTLGLAVEHIAQAKQSLDPFRESLEKGILASLADYVIN
                     RER"
BASE COUNT          830 a          975 c          773 g          811 t
ORIGIN      
        1 tacatttgag tacgcctccc tccgggagaa gccgcacacg ccttgatctt gaacaaaatc
       61 aacaggctgt cctaaaaaca gcagttcaac tgccgttttt aggttgatgt taccaatctt
      121 gttttctaaa tgtgtgacac gaccatgaac cccattcact tcacctgcag aatccctctc
      181 tcaaaactcc tgcccccacc gggcaaaggg ccacacctca catatgaggg gccgtccgtg
      241 agcgaactgc tgtcccccct gcttcaaaag ttgatgatcg gaagtttcag atacgcttgg
      301 aacctgaatg gccgggcact gagaacctgc gccaaaagat ttgcctcaca cgcgccaaaa
      361 atcgttttca cgattcgatc accctcaacc ccagctccag caattgatta gcatcgacct
      421 cacttgggca atcgctcatg agatcagagg ccttttgtgt tttgggaaaa gcgatcacgt
      481 cgcggatgtt cgtgcaaccc gtgagcaaca tcatgatacg atccaagcca aaggcaatcc
      541 ctccgtgcgg gggggcccca tactgcaagg catcgagcaa gaaaccaaat ttttgtttgg
      601 ccctctcttc gtcaatcccc agaagaccga aaactttgga ttgaatcacc tgatcatgaa
      661 tccggataga cccaccgcca atctcgttgc cattcagcac cagatcgtat gcgttggccc
      721 ggcacttgag gggctccgaa tccagcaaag gcatgtcctc tgtcttggga gaggtaaacg
      781 ggtgatgaat ggccacgtgg cgttttgcgg cctcatcgta atcaaacatc ggaaaatcca
      841 caacccacac gaacgcgtct ttgttattgt ctatgagtcc cctcttctca cccaggtgct
      901 cacgcaggtt gccaagacta tcatttacaa tttttttctt gcccgccccg aacagcatca
      961 cgtcaccggg ctctatgttg aacaggtcgg tcagtttttg tttctcggcg tccgaaaaaa
     1021 acttggcaat gggagaggcc cattcagtct cggtcacacg gatgtaggcc aaacccttgg
     1081 cgccgtacac cttgacgatt ttttcgagcg cgtcgatctg cgaacgcggc atgtcaccgg
     1141 cgtttttgag gttgatggcc ttgatcacag aatccccacc agcattggca atgtcatgaa
     1201 acactttgaa ctgagacacc ttgaacacat cggtgatggt tttgagttcc attccaaaac
     1261 gcatatcggg cgcgtccagc ccaaaacgct ccaggcactc atcgtacgtg atgcgggcaa
     1321 atggggtgtg gggtgcgatg cccttcacct cgtaccagat tttggcgatc aggccctcca
     1381 tgatcttgaa gagttcatcc tgcgtgatga aagaaagttc aaggtcgatc tgcgtgaatt
     1441 ccggttgtcg atctgcccgc aaatcctcat ctctaaagca tttgacgatc tgaaagtaac
     1501 ggtcaagccc gctcaccatg agcagttgct tgaagatttg tggcgactga gggagggcgt
     1561 aaaaactgcc cttctgcaca cgcgacggca ccagataatc gcgggcccct tctggtgttg
     1621 acttggtcaa acacggggtc tccacttcga caaagtgctg ttcattaaaa taattccgca
     1681 ccacatgaca cacctgcgag cgaatgatga tgttgttttt gattttgttg cggcgcaaat
     1741 cgaggtaccg gtacttgagc ctgatctcct cgtgcacatc aatgtcatcc acaatcatga
     1801 aaggcggtgt tttggagcgg ttgaagacct caaaatccag cacatcaacc tcgacctcgc
     1861 cggtcttgag attgaggttg gtcataccct ccggccggag attgaccttg cccttgatcc
     1921 ccagacaata ttcagtacgc agatgatgcc ccagttcgtg cacgcgggga tcgacttcgg
     1981 gatgaaacac aatctgggtg aggccaaacc ggtcgcgcag gtccacaaag atcagcccac
     2041 cgtgatcgcg ccgcgtatcg acccaaccaa acaacaccac ttccctgccc acatcctctg
     2101 tggagagttc atgacaataa tgcgtgcgtt gattcgttaa caaaaattca ctcataattc
     2161 acctttcccg gttgatcaca taatctgcca gtgatgccaa aatgcctttt tccaaggact
     2221 cgcgaaatgg gtccagactc tgcttggctt gagcgatgtg ctctaccgcc aaccccaagg
     2281 tgtcctcaaa accatgatac ttgcggatga tctggcgtac cctctcaaag agctcctggt
     2341 caatcttatc ggccaacacg gcatttttga tgacctgttt ttcgtctgga gccgcccggc
     2401 ccaatgacaa aatcaggggg agagtcagct tgccctccct gaggtcaatg ccctgggatt
     2461 ttccaaaaac gtcctcgctg gcattgtagt ctagaatatc gtccaccagt tgaaacgcca
     2521 tacccagatg caaaccataa tcactgagcg ctttgacaaa acaatcgctc acaccaccca
     2581 aaataccgcc cacctgacac gccgcctgga acaaaaaagc cgttttgagt ttgatgatgt
     2641 ttatatagtc atcgcggttg gtgtccagat cggaactttt gagggtttcc catatctctc
     2701 cctcagtcgt ggcgatggtg caatcggcca gcacctttaa aatgcgggtg ttgccctgct
     2761 caatcaacag ttgggacgca cgactgtaaa aaaaatcccc gaccagcacg ctgacgtgat
     2821 tgccccacaa agcgttcgcc gaggtcttgc ctcgccgaac cttggcgttg tcgatcacat
     2881 cgtcgtgcaa gagactggcc gtatgcaaaa actccacaca ggccccgact tgtgccgccc
     2941 tgttaccctg aaaacctgtc atcttcgcac acacaatcgt cagaatgggc cggatcctct
     3001 tgcccccgtt ttttaagatg tgctctccca cggttcgcac aaaggggatt tgggaatcca
     3061 gcaaaccaaa aatcgcctct tcaagcgcca gcagttcctg ctggaccgga aacagaatcc
     3121 ccttgagggc atcctgattt tgactccttt gtggggtttt ggaaaattcc atgggcgccg
     3181 attactatat gcattccctc atccttgtca aaataaagct cgttgatctc tcagctctct
     3241 cctcctataa agaaaaacgc actctcactc cccccaaatc aaccccctct atcttcccgg
     3301 caacaaaagt gccgcggtat tgtgcctgca cggtttcacg ggaacccctg actgcctgag
     3361 acacttgatc aatcatttga acgcgcaag
//
LOCUS       DATDUN010000292         1626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_596875, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000292 DATDUN010000000
VERSION     DATDUN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596875"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VJC18_04010"
     CDS             complement(<1..552)
                     /locus_tag="VJC18_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44575.1"
                     /translation="MSITTTCCGVSPQAILKSQPALSTAQVDAHLRALTDHAIREIVA
                     SDFAPLPNRRVSPTDRFLMLTALNGIRVDDPALDGQTLALGLTNRHSGNIYFQTAPVQ
                     RFHTTPFRVTYQDEQGGTLHYDHEDDPHAWDVGFGGTFIRQRSHHFNQSDADGNLLPV
                     SIITNPNTACSEACDGCSRVALGF"
     gene            complement(657..>1626)
                     /locus_tag="VJC18_04015"
     CDS             complement(657..>1626)
                     /locus_tag="VJC18_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLD44576.1"
                     /translation="YTSNPDVATGDGIAMGHRAGCAIANLEFVQFHPTCLYHPKAKSF
                     LISEALRGEGGRLKLIDGKSFMRKHHKLAELAPRDIVARAIDFELKKHGHEYALLDMT
                     HHPRSFLKKRFPTIYQTCRQFDCDMATTPIPVVPAAHYFCGGIKVDACGRTSIPNLYA
                     IGEVSCTGLHGANRLASNSLLEGLAFADFACQDLLTRKDLNQRQDLSIRNWDIGSATN
                     SDEAVVITQNWDEIRRLMWNYVGIVRTDKRLHRAQNRIAILKEEIRQYYWDFILTPDL
                     IELRNICLVAELIIESALKRKESIGLHYNLDHPTPSQNSKRFNLVK"
BASE COUNT          412 a          375 c          441 g          398 t
ORIGIN      
        1 aaatcccagt gccacacggg aacaaccgtc gcaggcctcg ctacaggcgg tgttgggatt
       61 ggtgatgatg gaaacaggca acagatttcc atcagcatca gattgattga aatgatgact
      121 gcgctgacgg atgaaggtgc ccccaaagcc cacatcccat gcatgaggat catcctcatg
      181 atcataatgt agcgtgccgc cttgttcatc ttgatacgtg acccgaaaag gagttgtgtg
      241 aaatctctgg actggggctg tctgaaaata aatatttccc gaatgcctgt ttgtaagacc
      301 cagtgccaaa gtttgaccat ctaaagcagg atcatcaact ctgatgccgt tgagggctgt
      361 gagcatcaaa aaacggtcgg tgggtgagac gcgtcgattg ggcagggggg caaaatccga
      421 cgcgacaatc tcacgaatgg cgtgatcggt cagcgctctt agatgggcat caacttgggc
      481 agttgataac gcgggttgtg attttaaaat tgcctgaggt gaaacaccac aacatgttgt
      541 cgtaatactc atggctgtca ctcctttgaa ctaaagtttt gtcgagtcaa aactttagta
      601 aaaaagataa aaaaacaaaa cattaatttt aagttgtagc aatttttcca aattactcac
      661 ttcaccagat taaaccgctt gctgttctga gagggggtag ggtggtcgag attgtaatgc
      721 aggccaatgg attccttgcg cttgagggcc gattcgatga tgagctcggc caccagacag
      781 atgtttctga gttcgatgag atcgggggtc aggataaaat cccaataata ttgacggatt
      841 tcttccttga ggatcgcaat tctgttctgt gcgcggtgca ggcgtttgtc ggtgcgcaca
      901 atgcccacat aattccacat gaggcgtcgg atttcgtccc aattttgcgt gatcaccact
      961 gcctcatcgg aattggtggc actgccgatg tcccagtttc ttatggagag atcctgtcgt
     1021 tgattcaaat cttttctggt cagcaaatcc tgacaggcaa aatccgcaaa ggccaagcct
     1081 tcgagtaacg agttggaggc cagacggttg gcgccgtgta gtccggtaca actcacctca
     1141 ccaatcgcat acagattggg aatggatgtt ctgccacacg cgtccacttt gattccgccg
     1201 caaaaataat gggcggcagg caccacaggg atgggcgtgg tggccatgtc acaatcaaat
     1261 tgcctgcagg tctggtaaat ggtgggaaac cgttttttta agaaggatcg gggatgatgc
     1321 gtcatgtcga gcagagcgta ttcatgacca tgttttttga gttcaaagtc aatggcgcgg
     1381 gccacaatgt cgcgcggggc caactcggcg agcttgtggt gcttgcgcat aaaactcttg
     1441 ccatcgatca gcttgagtct gccgccttca ccgcgcaggg cctcggagat gagaaacgac
     1501 ttggccttgg ggtgatacaa acaggtcggg tgaaattgga caaactccag attggcaatg
     1561 gcgcacccgg cgcgatgccc catggcaatg ccgtcacccg tggcaacatc gggattcgat
     1621 gtgtac
//
LOCUS       DATDUN010000293         1770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_220738, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000293 DATDUN010000000
VERSION     DATDUN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220738"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VJC18_04020"
     CDS             <1..1122
                     /locus_tag="VJC18_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006706802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLD44577.1"
                     /translation="HGKKVRCPEGYQAYVPDPLPPPIEWNDRLARHLSDADRLIGQLA
                     SIGAALPNPHLLIRPFVRREAVLSSRIEGTQTSLAELFADEAGVESQQDRSDLREVEN
                     YVKALEYGIKRLKTLPISLRLICEVHEQLMKGVRGNMATPGEFRRSQNWIGVAGCTLQ
                     NATYIPPPPHLLMDCLGALEKFFHESTLPPLVTIALIHLQFEAIHPFLDGNGRVGRLL
                     IILYLVERGVLPSPILYLSAFFEAKRDEYYARLRAVNENGDWEGWVEYFLNGVARQAE
                     DALGRIKRIQETVAKWKQSFTAKADKNCLLLIDDCMANPFMAVSGVAKKHKIAFPTAQ
                     RAIDKLEKTGVLKQTSDAKRNRIYCARKLLNILDEPAKV"
     gene            1170..>1770
                     /locus_tag="VJC18_04025"
     CDS             1170..>1770
                     /locus_tag="VJC18_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HLD44578.1"
                     /translation="MNTLIVSYLPSGERSNTKQLLDALLTRIPAINMERLDLLQDVPD
                     MFDELRLSAYIDRNYQNKTLSAEQQNAIQKMDRMTAQLKSADIVVIAYPMHNFSMPAM
                     IKAYFDSVMQKGVTWSIGSKGFEGLMKGKKALVLTSSGGVYEGPMVSWEHSTTLAKTE
                     FSFMGFSDVRVISAAGLNMDPQKTDQIIDDAKKQLRVAIK"
BASE COUNT          469 a          426 c          430 g          445 t
ORIGIN      
        1 catgggaaaa aggtcaggtg tccggaaggt tatcaagctt atgtccctga tccgcttcct
       61 ccgccgatag aatggaatga tcggctggca agacatcttt ctgatgccga tcgcctcatc
      121 ggtcagctgg cctcaatcgg agccgcccta cccaatcctc atctgttgat tcgtcccttt
      181 gtccgacgcg aagccgtctt gtcgagccgt atcgaaggaa cccaaacctc actggcggaa
      241 ttgttcgcgg atgaggccgg tgtggaatca cagcaagacc gttcggatct cagggaagtc
      301 gaaaactatg tcaaagcctt ggaatatgga atcaagcgtc tcaagactct ccccatttca
      361 ctgcgtctga tttgcgaggt ccatgaacag ctcatgaagg gtgttcgagg caatatggct
      421 acccccggag aattccggag atctcagaac tggattggtg ttgctgggtg cacgttacag
      481 aacgcgacct acattccgcc acctccgcat ctgctcatgg attgtctggg agctttggaa
      541 aaattctttc acgaatccac actgccgcca ctcgtgacga ttgctctgat ccatctgcaa
      601 tttgaggcga ttcacccatt tctcgatgga aacggtcgtg tgggacgcct gttgattatt
      661 ctgtatcttg tggagcgcgg tgttttacca tcaccaatct tgtacctcag cgcctttttt
      721 gaagccaagc gagacgagta ttatgcgcgc ctacgcgctg tgaatgaaaa tggcgattgg
      781 gaaggttggg ttgaatactt tctcaacgga gtcgcccgtc aggcagaaga tgcattaggt
      841 cgtatcaagc gaatacagga aaccgtcgca aagtggaaac aatcctttac ggctaaggcc
      901 gacaaaaatt gtttgttgtt gattgacgac tgcatggcga atccttttat ggccgtgagt
      961 ggcgtggcca aaaaacacaa gatcgccttt cctacggccc agcgcgccat cgataaactg
     1021 gagaaaacgg gtgtcctgaa acaaactagc gacgcaaaac gaaatcggat ttattgcgct
     1081 cgaaaacttt tgaatatctt ggatgagcct gcaaaggttt aaggtcagtg ggaatagtga
     1141 aatgacattt gtcttgtaaa ggggaattta tgaatacttt gattgtcagt tatttgccca
     1201 gcggtgaacg ttcaaacacc aaacaattat tggacgcatt gctgacacga atccctgcaa
     1261 ttaatatgga gcgattggat ctgctccaag atgttcccga catgtttgac gagttgcgac
     1321 tcagcgccta catcgatcgc aactaccaaa ataaaacact ctcggcagaa caacaaaacg
     1381 ccatccaaaa aatggatcgc atgaccgctc agctcaagtc tgccgacatt gtggtgatcg
     1441 cataccccat gcacaatttt tccatgcccg ccatgatcaa ggcctatttt gattcggtga
     1501 tgcaaaaggg agtcacctgg tctatcggat caaaaggatt tgaaggactc atgaaaggca
     1561 agaaggccct tgtcctaacc tcctctggtg gtgtttatga aggccccatg gtgagttggg
     1621 aacactccac cacccttgct aaaaccgaat tttcatttat gggtttttct gatgtcaggg
     1681 tgatttcagc cgctggcctt aatatggatc ctcaaaaaac agaccaaatc attgatgatg
     1741 cgaaaaaaca attacgagtt gccatcaaag
//
LOCUS       DATDUN010000294         1640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_348972, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000294 DATDUN010000000
VERSION     DATDUN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348972"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..727)
                     /locus_tag="VJC18_04030"
     CDS             complement(74..727)
                     /locus_tag="VJC18_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44579.1"
                     /translation="MTQGSTKHTSPRYFVLARISGLFGVFSLGLFLLILFSDTVTLLY
                     ADREIVQGLVRWQTTPFFIFFDTVLIILPLVGHLVFFIFRFFRCRLNVHLYPYPENLR
                     FVMLKIAGVLGLSFVGYHVGQLYGVYFEGMAGYQYLQTAIRAHQNLPTIILYWTGLAG
                     LGYYFFQLVWDQCIDFGICVSKTSQRVLTLVCLSGFLFYILVGLVLMIKAVFGAIEP"
     gene            914..1582
                     /locus_tag="VJC18_04035"
     CDS             914..1582
                     /locus_tag="VJC18_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HLD44580.1"
                     /translation="MFSFFRSSLGKKYLMAITGVPLILFLFAHMIGNLHIFLGPDALN
                     GYAHFLQGLGPLLWIFRMILITLFVVHVFVSATLVVENQLARPKAYSKKATLIATLSS
                     RTMIYTGTLIVACLVYHLLHFTVQSTDPIFQYLLDKEGRHDVYRMISLSFSYPTIAGA
                     YMSAVILLGFHLNHSASSFFQSLGLNNHRFHKYIQLIGPVTCLILVVGFSAVPFSILM
                     GWIR"
BASE COUNT          482 a          414 c          326 g          418 t
ORIGIN      
        1 cattgttaca ttcggagcca tggagccgta gaaccatggg gccgtggaac cgtagtccca
       61 tggctccatg gctctatggc tctatggccc caaaaaccgc cttgatcatc aggaccaacc
      121 caacaagaat ataaaataaa aaacctgaca ggcaaaccag ggtgagcacc ctctgggatg
      181 tcttgctcac acaaattcca aaatcaatgc attggtccca aaccaattga aaaaaataat
      241 agccaagccc cgcaagcccc gtccaataca gaatgatggt tggcagattt tgatgggccc
      301 tgatggccgt ctgcagatac tgataacctg ccatgccctc aaaatataca ccgtacagtt
      361 gtcccacatg ataccccaca aacgaaagtc ccagcacacc agcgatcttt agcattacaa
      421 aacgcagatt ttccggataa gggtacagat gcacattcag tcgacatcga aaaaaacgaa
      481 aaataaaaaa caccagatgc cctacgagtg gcaggataat caaaacggtg tcaaaaaaaa
      541 taaagaatgg tgtcgtctgc caacgcacaa gtccctgcac aatctctcga tcagcataga
      601 gaagcgtgac tgtgtcagaa aaaagaatca gcaaaaacaa ccccaaggag aaaacaccaa
      661 aaagccccga aatacgggcc aaaacaaaat accgaggcga cgtgtgctta gtagaaccct
      721 gagtcatatc aatagtttag tttattcaac aaaccccacc aatggtcaag gattaaatgg
      781 tttatggcac aacccattac ccatcaacca tatgacgttt gtcattgaaa cacgcctcga
      841 taaatactaa ccagtcgcca cttcatgcga tcctgccaca tcgtttttct gaccacacaa
      901 tatgggagcc aacatgtttt ctttttttag atcctcacta ggcaagaaat acctcatggc
      961 catcacgggc gtacctctca ttcttttttt attcgcccac atgattggca atctgcatat
     1021 ttttttgggg cccgatgcac tcaacgggta cgcgcatttc ttgcaaggtt tgggcccatt
     1081 gctttggatc ttcagaatga ttctcattac cctgttcgtg gtacacgtct ttgtatcggc
     1141 gacactcgtt gtggaaaatc aattagcccg accaaaggcc tattcaaaaa aagcaacact
     1201 gatcgccacc ctttcctcgc gtaccatgat ttatacgggt accttgattg tggcctgtct
     1261 ggtctatcac cttcttcatt ttactgtgca atccactgac cccatttttc aatatctgct
     1321 ggacaaggag ggacggcatg acgtgtaccg gatgattagc ttgtctttct cttatcccac
     1381 aatcgccggt gcgtatatgt ctgctgtgat tttgctgggt tttcacctca accacagcgc
     1441 ttccagtttt tttcagagtc tgggactgaa caatcaccga tttcacaagt acatccaact
     1501 gattggtcct gtgacctgtc tcattttggt tgtgggattt tcagccgtac cgtttagtat
     1561 tctgatggga tggatacgat aaatacgtag atcgtggatc gtgtatcgtg aatcgtgaat
     1621 cgaaaataaa tttaacacga
//
LOCUS       DATDUN010000295         3591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_388308, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000295 DATDUN010000000
VERSION     DATDUN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388308"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1611)
                     /locus_tag="VJC18_04040"
     CDS             complement(154..1611)
                     /locus_tag="VJC18_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLD44581.1"
                     /translation="MNTTLTIDKNIRKKAIDRCREKGVILPTFAQQRDPTTIPDAIKI
                     KLKNIGLWDINSLNLFRITWKNEIKAGGFGAVNFLEIPQAITGVKARIIGLVGKYFPT
                     GAHKVGASYGCLVPRLVTGGFDPTTQKAVWPSTGNYCRGGAYDAALLGCESVAILPQE
                     MSRERFDWLKKIGSEIIATPGCESNVKEIYDKCWELKKERGDKVVILNQFDEFGNCMW
                     HYAVTASAIDEVLRSQLGPKDHLFAYISATGSAGTIGAGDRLKELYPHMKVVASEALQ
                     CPTLYNNGYGAHRIEGIGDKHIPWIHNVKNTDVVTAIDDEACMHVMRLFNEDAGKTYL
                     SELGVNKDVIERLSLMGISGIGNMLSAVKTAKHFELTERDVLFTIFTDSMEMYGSRLD
                     EERARLGAYSHEKAAVDWNRHLAGFGTDHLQELTYADRKRIHNLKYFTWVEQQGKDVA
                     ELNAQWYDREYFPNHLRRADEYDRMIAEFNKEVGL"
     gene            1740..1910
                     /locus_tag="VJC18_04045"
     CDS             1740..1910
                     /locus_tag="VJC18_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44582.1"
                     /translation="MPYTNGHGIRVYYFVELFEFFYGSGFQTPERAQAIANKQISALL
                     NVSTVIFSIHCT"
     gene            complement(1898..3139)
                     /locus_tag="VJC18_04050"
     CDS             complement(1898..3139)
                     /locus_tag="VJC18_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44583.1"
                     /translation="MNKHVVNVMGLFLLVLCLYGSNAVALYIEVQARTYTQVSAGDRH
                     SCGLADDGSVWCWGQGNDGKLGNGDVVDQDQPVQVINSSGNVMKDFKQVSAGSFHTCA
                     LRADTSVWCWGAGEDGQMGNGDSSNQISPVQVMNSDGRAMKGFSLISAGNDHTCGVRN
                     NSTIWCWGNNRYGQIGNKANTDVYIATQVVNELGKVNKGFVQVSAGAYHSCALKDDRS
                     VWCWGYGDFGQLGNGGEVSSNVPVAVVEISDIKVKITDFDAVSAGGYHTCALKADSGS
                     LWCWGMGNNGQLGNGQIAHQASPVRVVSASHQKMADFIDVSAGEYHTCGMTSDDAVWC
                     WGQGDSGQLGNGNTDDVNGAVLVLENDENTEIGFGQLSVGGEHTCGFKDEGSIWCWGL
                     GREGQLGNADVSNHSTPVQVQ"
     gene            complement(3283..>3591)
                     /locus_tag="VJC18_04055"
     CDS             complement(3283..>3591)
                     /locus_tag="VJC18_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44584.1"
                     /translation="PAAGVFRSHQQIGNLVQSGDVLGEVAGQPITAKIAGVLRGLIMP
                     GIEVSAEQKLGDIDPRGDVSYSRTISDKARTISGSVLEVVVSYENSCLIQNSQNGKNV
                     "
BASE COUNT          855 a         1035 c          770 g          931 t
ORIGIN      
        1 caaccccggc aggggttgct tatcgtagcc gtgggcttta gcccacggtc agtatgcaaa
       61 tcccatttcc aaccccgtca ggggttgcga atcgtagccg tgggcttcaa cccacggtca
      121 gtatgcaaat cccatttcca accccgtagg gcatcacaac cccacctctt tattaaattc
      181 cgcaatcatg cgatcgtatt catcggcgcg ccgcaggtga tttggaaagt actcgcggtc
      241 ataccactgc gcattgagtt cggccacatc cttgccctgc tgttcaaccc atgtaaaata
      301 ttttagattg tgaattcgtt tgcgatcagc atacgtcagc tcttgcaagt gatccgtgcc
      361 gaaacccgcc agatgcctat tccaatcgac tgccgccttt tcgtgactgt acgcccccaa
      421 acgcgcccgt tcttcatcca aacgagaacc gtacatttcc atggagtcgg taaaaatggt
      481 gaacagcaca tcacgctctg tgagttcaaa gtgcttggcc gttttgacag ccgagagcat
      541 gttgccaatt cccgaaattc ccatgaggga tagcctctct atcacgtctt tattcacgcc
      601 cagttcggac agatacgttt tgccagcatc ttcattgaag agtctcatca cgtgcatgca
      661 ggcctcgtca tcaatggccg tgaccacatc ggtgtttttg acgttgtgaa tccacgggat
      721 gtgtttgtcg ccaatgccct caatgcgatg cgcgccataa ccattattgt aaagtgtggg
      781 acactgcagg gcctcgctcg ccaccacctt catgtgcggg tagagttctt taagcctgtc
      841 gcccgctcca atcgtgccag ccgaacctgt ggccgaaatg taggcaaata aatgatcctt
      901 gggtcccagt tgcgagcgca aaacctcgtc gatggcgctg gccgtaacgg cgtagtgcca
      961 catgcagttg ccaaactcgt cgaactgatt gaggatcacc accttgtcac cgcgttcttt
     1021 tttgagttcc cagcatttgt cgtaaatttc tttgacgttc gattcgcaac ccggagtcgc
     1081 gatgatttcg gaaccaattt ttttaagcca gtcaaatcgt tcacggctca tttcttgtgg
     1141 cagaatggcc acggactcgc aaccgagaag tgctgcatcg tacgcgccgc ccctgcagta
     1201 attgcccgtg gatggccaca cggccttttg cgtggtgggg tcaaacccgc cggtgaccaa
     1261 acgtgggacc agacaaccat acgaggcacc caccttgtgg gcccctgtgg gaaaatactt
     1321 gcccaccaga ccaatgatgc gtgccttcac acccgtgatg gcctgcggga tttccaaaaa
     1381 attcaccgcg ccaaacccgc cggctttgat ttcgttcttc cacgtgatgc gaaaaaggtt
     1441 cagcgaattg atatcccaaa gccctatgtt tttgagtttt attttgatgg catcagggat
     1501 tgtggtgggg tctctttgct gggcaaatgt gggcaggatc acaccctttt cgcgacaacg
     1561 atcaatggct ttttttcgaa tgtttttatc aatagttaat gtggtgttca taaaaggttc
     1621 cttacgcgaa ttaacaaaat tttgcgctag ttatgaccgc agggccccaa aatcaaggca
     1681 tttttgtctg gaccggataa ttgacttgat cacaatagct gtctacagtc gcccggtcta
     1741 tgccctatac caatggccac ggaattcgcg tgtattattt tgtggagctc tttgaatttt
     1801 tctatggaag tggttttcaa acccccgaac gggcacaggc catcgccaac aaacaaatat
     1861 ccgcgctatt gaacgtctca acggtcattt tctcaattca ctgcacctga actggtgtgc
     1921 tgtgatttga gacatcggca tttcccaact gtccctccct accaagaccc caacaccaga
     1981 tggaaccctc gtctttgaat ccacaggtgt gttcaccccc tacgctcaat tgtccaaaac
     2041 caatctcagt attttcatca ttttccagca ccaacacagc gccgttcaca tcatcggtgt
     2101 taccgttccc caactggccg gagtccccct gaccccagca ccaaacggcg tcatctgagg
     2161 tcattccgca tgtgtggtat tcacccgcgc tcacgtcaat gaagtccgcc atcttttgat
     2221 gagaagcaga gaccacacgc acgggagaag cttggtgtgc tatttgaccg tttcccagtt
     2281 ggccgttatt gcccattccc cagcaccaga gtgaaccact atcagccttt aacgcacagg
     2341 tgtgataccc gcctgcgcta accgcatcaa aatctgtgat cttgaccttg atgtcggata
     2401 tttcaaccac ggcaactgga acgttgctac taacctcgcc cccatttccc agttggccaa
     2461 aatcaccata cccccaacac cagacagacc ggtcatcctt gagggcacaa ctgtggtagg
     2521 ctcccgcact aacctgaaca aaccctttat tgactttgcc caattcattg accacctgcg
     2581 ttgcgatata gacatctgta tttgccttgt tcccaatctg tccgtatctg ttatttcccc
     2641 aacaccaaat tgtgctgttg tttctgacac cgcaggtatg atcattaccc gcgctgatca
     2701 gagagaatcc cttcatcgcc cgaccgtcag aattcattac ctgcacaggc gatatttggt
     2761 tagatgaatc gccgttgccc atttgcccgt cctcaccagc accccagcac caaacagagg
     2821 tatccgccct caacgcacac gtatgaaatg acccggcact cacctgtttg aaatccttca
     2881 tcacgttccc gctggagttt atcacctgaa ctggttgatc ttggtcgaca acatctccat
     2941 tacctaactt gccatcattg ccttggcccc aacaccagac agacccatca tccgccaatc
     3001 cacacgaatg cctgtccccc gcactcacct gagtgtatgt tcgagcctga acctctatgt
     3061 aaagcgcaac cgcgttactt ccatacaaac ataatactaa caggaacagt cccataacat
     3121 tgacaacgtg tttgttcata caacctccca tggcgtgatg accacctctg aaccagcaag
     3181 ataaatgcca aagtcagaag atatatttga caatgatttc agatagatat tacctatatc
     3241 cgttgataga tcaggcattt ttttgacagg tattatttaa aatcagacat tcttaccatt
     3301 ttgagaattt tgtataagac agctgttttc ataactcacc accacctcta acacgctccc
     3361 actgatagtg cgcgccttgt cggagatggt tctggaataa gaaacatcgc ctctgggatc
     3421 gatgtcacca agcttttgtt ccgcagaaac ctcgatgccc ggcatgatga ggccgcgaag
     3481 gacgcctgcg atctttgcgg tgatgggttg tcctgccacc tcgcccagca catcacctga
     3541 ttgaaccaga ttgcctattt gctggtggct cctaaatacg cctgcggcag g
//
LOCUS       DATDUN010000296         1973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_16297, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000296 DATDUN010000000
VERSION     DATDUN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16297"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VJC18_04060"
     CDS             <1..151
                     /locus_tag="VJC18_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLD44585.1"
                     /translation="VLSIFSAKAPVLIPLIFLGFGVFVSFIQAFVFTLLSTVYIGLAV
                     ETHEH"
     gene            390..695
                     /locus_tag="VJC18_04065"
     CDS             390..695
                     /locus_tag="VJC18_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HLD44586.1"
                     /translation="MKSVSFILSTLIGLFITGLAFASGDAETATSSLNLAPIGAGLIM
                     AFASGLGTLAQSKAAAAALEGIARNPEAASKVQTPMIIALAFMESLVIFAFIIAITL"
     gene            complement(861..1037)
                     /locus_tag="VJC18_04070"
     CDS             complement(861..1037)
                     /locus_tag="VJC18_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44587.1"
                     /translation="MRNRENELQIVTIDEDIRLEHDEDALTNRERLEIYNRYYELEID
                     RLNLEYQDLNPTIH"
     gene            1520..>1973
                     /locus_tag="VJC18_04075"
     CDS             1520..>1973
                     /locus_tag="VJC18_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44588.1"
                     /translation="MKTQNHKHWPLVIMLAGVTFLVLTLYPRTSLSQTGGLQRKELSA
                     VGEPNNNNATVFELKEKAIERIFQLNEKCGDRIDNNHDNTIDEGCCGNGSLDRPAEEC
                     DTTTAVPNAHCNQCEVVCDSGYTKTMVNNRIACVAEAICGDGSKNQPTE"
BASE COUNT          480 a          486 c          437 g          570 t
ORIGIN      
        1 ggtcctttcc attttttctg ccaaggcgcc tgtcctcatc cccctcatct ttttgggctt
       61 tggcgtcttc gtttcgttca ttcaggcctt tgtgtttaca cttctgtcca ctgtatacat
      121 tgggctggcg gtggaaacgc atgaacattg aatccatcag tgaactgtgc gtaataatag
      181 cgggtgatac ggggagctat tattttggcg cggcctgcgg agcgagcttg cgagcggaga
      241 gcgtcaaaaa atagcgaccc gaagcaggac ccgcattgat tattacgcac agttcactga
      301 tgggttgcca tgagtattga atcaacaatt gacaaaacgc tcacaaaatt aaatattcac
      361 acacgttttt ttacaaggag ccgcacacta tgaaatctgt ttctttcatc ctttctacac
      421 tcattggtct tttcatcacc ggcttggcct ttgcttccgg tgacgccgaa acagccacct
      481 ccagcctcaa cctagcgccc attggcgctg gcctgatcat ggcctttgcc tcggggttag
      541 gcacactggc ccagtccaag gcggccgccg ccgcactcga gggcattgcc cgcaatcccg
      601 aagcggccag caaggttcaa acccccatga tcattgcttt ggcttttatg gaatctttgg
      661 tgatctttgc cttcatcatc gccatcacgt tgtagtgcat acggcacaac agaccttcat
      721 tggcctctgg caactatttt tttgatattt gtttttgttt tgtggatcgg cacggttcgc
      781 tgggctcgcc tcagaatgac aacccatgtc tcaccttttt tgccttacgc cttacgcctt
      841 ttgccttgcg ccttacgcca ttaatgaatc gtggggttca aatcttgata ttcgagattc
      901 aggcgatcga tttccaattc gtaataccgg ttgtagattt cgagcctctc gcggttggtc
      961 agtgcgtctt cgtcatgttc gagcctgatg tcttcgtcga tggtcacaat ttgcaattcg
     1021 ttttctcgat ttctcataat gtacctctca accatattat cgtcacacga aacctgtttg
     1081 ttgctattta tttttaatac ttgatctttt gtcgcgattt tcaatgagtg acaacggtga
     1141 acagcagatg acaaaagaga tctcttgaat cgccattgct tagccgtgcg ctgtggtgac
     1201 attgccaagg gcaatgttag cgttgtgcgc cgccgtgggg tctccttgtt gtagggagat
     1261 cgccacgatt cactacatga atctcgcgat gacgagaggc ttacgctccc cgccctacgc
     1321 cttacacctt gcgccttacg cctttttcgt aaacacctga acacacccag taccataatg
     1381 gtactgggca acatttgttt cccattttga taatcctatc gaaaagagaa cccctgtccc
     1441 cctcataccg tcctaatcat ttgattaatc agagtttttt tgtgtcaaaa ctggcataaa
     1501 ccttgcttct aagaacaaca tgaaaacaca aaatcataaa cattggcctt tagtaatcat
     1561 gttggcaggc gtcacttttt tggtgctaac attgtatcca cgaacgtctt tgtcgcaaac
     1621 aggcggactg caaagaaaag aattaagcgc tgtcggtgaa cctaacaata ataatgcgac
     1681 tgtgtttgag cttaaagaaa aagctataga aaggattttt caacttaatg aaaaatgtgg
     1741 cgatcggata gataataatc acgataatac gatcgatgaa ggctgttgtg gtaatggtag
     1801 cctagatcga ccggcagagg aatgtgatac cacaactgcc gtaccaaatg cccactgcaa
     1861 tcaatgtgag gtcgtatgtg attcgggtta cacaaaaact atggtgaata accgcatcgc
     1921 ctgtgtcgcg gaagctatct gcggtgatgg aagcaagaat caacccaccg aag
//
LOCUS       DATDUN010000297         1563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_512536, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000297 DATDUN010000000
VERSION     DATDUN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512536"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VJC18_04080"
     CDS             complement(<1..449)
                     /locus_tag="VJC18_04080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HLD44589.1"
                     /translation="MPIFIVKNNPSLANPIKLDKDDEHHLLRVMRMKPGSRVTLTDNQ
                     GTLALACLDHISPVVLTRLSTSQQPPPPPLVVCLPLIEQRHMEWAVEKITELNAGTIQ
                     LITTARCQQQTLSARKMNRLEKVALAAQKQSERACPLQILDPVPLKK"
     gene            complement(424..1311)
                     /gene="prmA"
                     /locus_tag="VJC18_04085"
     CDS             complement(424..1311)
                     /gene="prmA"
                     /locus_tag="VJC18_04085"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLD44590.1"
                     /translation="MSTQKQQNWLEAKWLIDPSNTELFQERLEQYGSLGSYENLPMEP
                     TGQETDIELISYFPDTADVNALAAQLQSLENNTTRLLTVKKIPQGNWATEWKKYFKPF
                     LFADNIVIRPSWEPYTPKQNEIVLTIDPGMAFGTGQHDTTQFCASLICKIKKQRPELS
                     TLIDVGCGSGILSLLASLIGFQKVVGFDVDETAIETAFENLEKNPSVRNVDFRLSKGT
                     ISELFLHPADVVAANIIAETLVELRDDLVSLVAKNGCLILSGILPDKDELIKKSFSDL
                     VLCEMAQSQNWYAYLYCKK"
BASE COUNT          382 a          340 c          399 g          442 t
ORIGIN      
        1 aattttttga gaggcacagg atcaagaatt tgtagcggac aggcgcgctc tgattgcttt
       61 tgagcggcca gcgccacttt ttctaggcga ttcatcttac gtgctgatag tgtttgttgt
      121 tggcaccgtg ctgttgtgat gagttgaatg gttccggcat tgagttcagt aattttttcc
      181 acggcccatt ccatgtgtcg ttgctcaatg aggggtagac aaaccacgag aggtggaggg
      241 ggaggttgct gtgatgttga cagtcgtgtc aggaccacag gggaaatgtg atcgaggcaa
      301 gccaaggcaa gagttccctg attgtctgtc agggtaacac gggaacctgg tttcatgcgc
      361 atcacgcgca acaggtggtg ttcatcatct ttgtcgagtt tgatgggatt tgcgagggag
      421 gggttatttt ttacaataaa gataggcata ccaattttgt gactgcgcca tttcgcaaag
      481 gacaagatca gaaaaacttt ttttgatgag ctcgtctttg tcgggcaaaa ttccggataa
      541 gatcaggcaa ccattttttg cgaccaacga cacaagatca tcgcgcaact ctaccaatgt
      601 ttcggcaatg atgtttgccg ccaccacatc cgccggatga aggaaaagtt cactgatggt
      661 gcctttgctg agtctgaaat caacattacg gactgaggga tttttttcca gattttcaaa
      721 tgcggtctca atggctgttt catccacatc gaaccccacg actttttgaa acccaatcag
      781 gctggccaat agggacaaaa tgccgctccc gcaaccgacg tcaatcaagg tggatagttc
      841 aggtctttgt tttttgattt tgcaaatcag actcgcacaa aactgtgtgg tgtcgtgttg
      901 ccctgtcccg aacgccatgc ccggatcgat cgtgagaacg atttcatttt gtttgggtgt
      961 gtagggttcc cagctggggc ggatgacaat gttatcggca aaaagaaaag gtttgaaata
     1021 ttttttccat tcggtggccc agttgccctg cgggattttt ttgacggtca acaacctggt
     1081 tgtgttgttt tcgagggatt gtaattgagc ggccagagca ttgacatcag cagtgtctgg
     1141 aaaataggag atcagctcga tgtctgtttc ttgtcctgtg ggctccatgg ggagattttc
     1201 ataactgccc agggagccgt attgttccag cctctcctga aagagttcgg tgtttgaggg
     1261 atcaatcagc catttggctt ccagccagtt ttgctgtttt tgtgtgctca cgaacacttt
     1321 tctaacgcac gcacgcacgt acgtcaacac gtacgcacgc aaaaatgctt gtcatcttgt
     1381 ctggtgccta atagtgaagc aacatggaaa acacaacccc cccaacaaat aacgtccaag
     1441 tcatgtgcga atgggaagcc aattacaaga cggatccctt gcggtcatat gttaatacgg
     1501 tgtggcaggt catattacac ccagtaagtt attttgccac gatcaaaccg tttcaaaact
     1561 att
//
LOCUS       DATDUN010000298         1697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_220870, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000298 DATDUN010000000
VERSION     DATDUN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220870"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VJC18_04090"
     CDS             complement(<1..765)
                     /locus_tag="VJC18_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLD44591.1"
                     /translation="MQILSSKYLVTMLGEPVFDGAVAVEGGEIIDVGREQDLLTRYSG
                     AVHEDYPNHVIMPGLINCHTHLDMSFYKDFPFDPVRTGGLTVNFIEWLLGTIQYKKTA
                     EPGQKHQSIEWAVDECLQSGTTCVADMSSYEGIFNILEQKNMRAVVFPEVLSIDNAVT
                     KELFESAMAIIEKYADYDSDLVSVGAGPYSPYMLSRNLLRIMSQVSCSSQIPIMIHVA
                     ESFCEMEFFHNSTGDIATKLFPNIGWDDLPPEHHRTP"
     gene            complement(801..1640)
                     /gene="panC"
                     /locus_tag="VJC18_04095"
     CDS             complement(801..1640)
                     /gene="panC"
                     /locus_tag="VJC18_04095"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HLD44592.1"
                     /translation="MKRVTSIAVMQKLAREIKQKNTIALVPTMGFLHEGHLSLVRLAI
                     KKADVVIVTIFVNPTQFGPHEDFQKYPRDTNSDLQKLGKLDVDYVFVPTSESMYPEGH
                     QTSVGVSRLTQPLCGKSRPGHFDGVALVVNKLFNITQPDFAVFGLKDYQQLVVIERLV
                     KDLNMPITIVRGKTIREKDGLAMSSRNAYLNPQERQLAKALNRGLSKIKKVCANGACT
                     VLQMKTQFYSEIPDDHRVRVDYLEVLDANRLTLIKKYQRNNTLVAAAVFVGKTRLIDN
                     LVF"
BASE COUNT          428 a          451 c          384 g          434 t
ORIGIN      
        1 aggggtacgg tgatgctccg gcggcagatc atcccaacca atgttgggaa acagtttggt
       61 ggcgatgtcc ccagtggaat tatgaaaaaa ctccatctca caaaaactct ccgccacatg
      121 aatcatgatg gggatttggg acgaacagct cacctgcgac atgatgcgca acaagtttcg
      181 cgacaacatg tatggcgaat agggccccgc ccccacactg accagatcgg aatcatagtc
      241 cgcgtatttt tcgatgatgg ccatcgcact ttcaaaaagt tccttggtca cggcattgtc
      301 aatgctcagc acctccggaa aaaccacagc acgcatgttt ttttgttcaa gaatattgaa
      361 gatgccctca taactgctca tatcggccac acaggtcgtt cctgattgaa gacattcatc
      421 gacagcccac tcgatggatt ggtgcttctg ccctggttcg gcggtctttt tgtactgaat
      481 cgttccgagt aaccactcga taaaattgac cgtcaacccg cccgtacgca cggggtcaaa
      541 agggaaatcc ttgtaaaaac tcatgtcgag atgggtgtga cagttaatga gtcccggcat
      601 gatcacatga ttggggtaat cctcgtgaac cgcccctgag tagcgcgtca agagatcctg
      661 ttcgcgaccc acatcgatga tctcgccccc ctccacggcc acagccccat caaagacagg
      721 ctcgccaagc atggtcacaa gatattttga tgataaaatc tgcatcggct caccctcaaa
      781 cagtcgccag cgactgtcaa tcagaatact aggttatcaa tcaaccgcgt ttttcccaca
      841 aaaaccgccg ccgccacgag cgtgttattt ctttgatatt tttttatcaa ggtcaagcga
      901 tttgcatcca atacttcaag gtaatcgaca cgcacccggt ggtcatcggg gatttcacta
      961 taaaactggg ttttcatttg caaaacagta caagccccgt tcgcacaaac tttcttgatt
     1021 ttggacaaac cacggttgag cgctttggcc aattgccgct cttgcgggtt caaataggcg
     1081 ttacgtgagc tcatggccaa tccgtctttt tcgcgtatgg ttttgccacg cacaatggtg
     1141 atcggcatgt tcaggtcttt gactaggcgc tcaatcacca cgagttgttg ataatccttg
     1201 agaccaaaga cggcaaaatc aggttgtgtg atgttaaaca gcttattgac caccaacgcc
     1261 acaccatcaa agtgtccggg ccttgacttc ccacacaacg gctgcgtgag ccgtgacaca
     1321 cccacgctgg tttgatgccc ttcgggatac attgattcgc ttgtgggaac aaacacgtaa
     1381 tccacgtcca attttccaag tttctgcagg tcactatttg tgtctcgcgg gtatttttga
     1441 aaatcttcat ggggtccaaa ttgggtgggg ttgacaaaga tcgtcacaat cacaacatcg
     1501 gcctttttta tggccagcct gaccagagac aaatgcccct cgtgcaaaaa acccatcgtt
     1561 ggcaccagcg caatggtgtt tttttgtttg atctccctgg ccagtttttg catgacagcg
     1621 atggacgtga cccttttcat gggcgatccc ttagcccgat aagtccaact aatcaaagac
     1681 tatttgacgt cggcctg
//
LOCUS       DATDUN010000299         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_471028, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000299 DATDUN010000000
VERSION     DATDUN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471028"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VJC18_04100"
     CDS             <1..1172
                     /locus_tag="VJC18_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLD44593.1"
                     /translation="WTQAFSLLAKYVRTGRAVLLFDEISWMAAGDKTFSAKLKIAWDT
                     LFKTNPKLVLVLCGSVTSWMDQNILNNAAFVGRVSLTLTLQELPLHHCNEFWGKKKNT
                     ITSAEKFKILSVTGGVPRYLEEIKTNLSAEQNIKRLAYQPAGFLFSEFDNIFNSTFSR
                     RAPVYREIVTTLVSGSKTFTEICHILKRDNNGTMSHYLNDLIESGFLKQDFVFSTERK
                     KNGVSKYRLSDNYLRFYLKYIEPAKEQIKGGLYATVSLETLPGWDVIMGLQFENLILN
                     NLPALCGHLLVPLNSVVSCSPYFQKTTQRKQACQIDLLIQTKNTIYVCEIKFKKNISA
                     SVIKEVQDKIVKLKTPKSVTIRPVLIYCGCLATELKYDDFFSHKINADAFLTALS"
     gene            1555..1815
                     /locus_tag="VJC18_04105"
     CDS             1555..1815
                     /locus_tag="VJC18_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLD44594.1"
                     /translation="MLKLLGENIRLARLRRHLSATRLAERAGMTRMTLRAIEHGEPSV
                     SLGAYANVLVSLGLEKDLSLVAKDDELGRKLQDADLLAHKLS"
     gene            1819..>2414
                     /locus_tag="VJC18_04110"
     CDS             1819..>2414
                     /locus_tag="VJC18_04110"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HLD44595.1"
                     /translation="MIWARSKRNKNYASWLNLLIAPGGSLGGARPKASVIDARGELWI
                     AKFPSRNDELNVGAWEFVVHKLAGLAKITTAEAQIKKFSSPHDTFLTKRFDRQQKQRI
                     HFASAMTLLGKKDGECADTGASYLDLVEFLMQQGSQVQKDLEELWRRIVFNICVSNVD
                     DHLRNHGFILTEKGWRLAPAYDMNPSETGDGLKLNISK"
BASE COUNT          715 a          522 c          547 g          630 t
ORIGIN      
        1 attggacgca ggctttctca cttttggcaa aatatgttcg cacgggcagg gctgtgcttt
       61 tgtttgatga aatatcgtgg atggccgcag gcgacaagac cttttctgcc aaactcaaaa
      121 ttgcctggga taccctgttc aaaacaaatc caaaactcgt tcttgttctc tgtggttcgg
      181 tcacctcctg gatggaccaa aatattctca acaatgccgc ctttgtcggg cgtgtttcac
      241 tgacgctcac tcttcaggaa ttacccctgc atcactgcaa tgaattttgg ggcaaaaaga
      301 aaaacacaat tacgagtgcc gaaaaattca agattttgtc ggtgacgggc ggtgttccgc
      361 gatatctcga agagatcaaa accaacctat cagctgaaca aaacataaaa aggctggctt
      421 atcagcctgc aggatttctg ttttcagaat ttgacaatat ctttaactcc actttttcta
      481 gacgcgcccc tgtttacagg gaaatcgtca ctaccttggt cagcggaagc aagacattca
      541 cagaaatctg ccacatttta aagagagaca acaatggcac catgtcgcat taccttaatg
      601 atttgattga atccggtttt ttaaaacagg attttgtttt tagtacagaa agaaaaaaaa
      661 atggggtcag caaatacagg ctctctgata actatctccg gttttatctg aaatacattg
      721 aaccggccaa agaacagatc aagggcgggt tgtatgctac ggtgtcattg gagaccttgc
      781 cggggtggga tgtgatcatg ggtttgcagt ttgaaaatct tattctaaac aacctgcctg
      841 ccctttgtgg tcatttattg gtgcccttga actcggtggt gagttgttca ccctattttc
      901 aaaaaacgac gcaaagaaaa caagcctgcc agattgattt gctcattcaa accaaaaaca
      961 cgatttatgt ttgcgagata aaattcaaaa aaaacatctc tgcttcggtc atcaaagagg
     1021 tgcaggacaa aattgtaaaa ttaaaaactc ccaagtcggt gacgatcaga ccggttttaa
     1081 tttattgtgg gtgcctggca acagaattaa aatacgatga ttttttcagt cataaaataa
     1141 acgccgatgc cttcctcacc gccctctctt gattgaatga tcgttacccg caagcatgtt
     1201 gtcctgggca gtttttaccc catcaaatcg acaaaataat gactcctttc aacatcaaaa
     1261 cgcgtcattg ttgattctgc gtaagaagcg actttctaac ccaacttgcg cagttagcgt
     1321 agtctgagaa cgataaatca ataggttagc cgaaaagtcg gcactaaaga ggtagggtca
     1381 aagattgaca agagacaagt tgtgacaaca aattcacaat gtgaagagtt attgatcctg
     1441 aaaaatccca ttaacgttaa gaaaagtata atatgattgc caaaaagtat agatttgtat
     1501 attatattat acgtgcctaa gaaaacatca ccccaaatgc ccaagcttgt ttctttgctc
     1561 aaattgcttg gtgaaaacat ccgtctggca cggctacgac gccacctttc tgctaccagg
     1621 cttgccgaaa gggcgggaat gacccgaatg accttgaggg cgattgaaca cggagaacct
     1681 tctgtgagct tgggggccta tgccaatgtg cttgtcagtc tggggcttga aaaagatttg
     1741 tcacttgtcg ccaaagatga tgagttgggc cgaaaattgc aggatgctga tttgctcgca
     1801 cacaagttgt cctgaaaggt gatatgggca agaagcaaac ggaacaaaaa ctacgccagc
     1861 tggctcaacc ttctcattgc ccccgggggg tctctgggag gggcccggcc caaggcgagt
     1921 gtgattgatg ccagaggaga attgtggatt gcaaaatttc caagcagaaa tgatgaactc
     1981 aatgttgggg cctgggaatt cgttgtgcac aaacttgcgg gcctggccaa aatcaccaca
     2041 gctgaggcac aaatcaaaaa gttttcaagt ccccatgaca cttttctcac caaacggttt
     2101 gatcgccagc agaaacaaag aatccatttt gcttcggcca tgacactgct tggtaagaag
     2161 gatggagaat gcgctgacac gggggctagt taccttgacc tggtggaatt cctgatgcaa
     2221 caagggtcgc aggttcaaaa agatttggag gaactctggc gcagaatcgt ctttaatatt
     2281 tgcgtgtcca atgttgatga ccacctgaga aaccacggat tcattttaac agaaaaaggt
     2341 tggcggcttg cccctgccta cgacatgaat ccttccgaaa cgggggatgg tctcaaactg
     2401 aatatttcaa aaga
//
LOCUS       DATDUN010000300         1729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_512760, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000300 DATDUN010000000
VERSION     DATDUN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512760"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..467
                     /locus_tag="VJC18_04115"
     CDS             228..467
                     /locus_tag="VJC18_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HLD44596.1"
                     /translation="MKKKIKYTDESLGDIKLVSDHFPKPHELVFKEDTVKVTIELTKN
                     SVAFFKEQAKKHHTHYQAMIRTLLNYYSTICGNER"
     gene            complement(518..754)
                     /locus_tag="VJC18_04120"
     CDS             complement(518..754)
                     /locus_tag="VJC18_04120"
                     /inference="COORDINATES: protein motif:HMM:NF027581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HLD44597.1"
                     /translation="MFYLFYQVNKSDIHYLKHILEAYENMIQVSTVDEALPKLQLSIA
                     PDFVDDVLGIIQNLQKSFFLKQLDDDPHVSQGNY"
     gene            complement(906..>1729)
                     /locus_tag="VJC18_04125"
     CDS             complement(906..>1729)
                     /locus_tag="VJC18_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLD44598.1"
                     /translation="RRGKSKARITNYESLLSQSNEQYNDDLQIFIPPGPRLGAKVIEA
                     NKVSKAFGDKLLFENLEFNLPQGGIVGVIGPNGAGKTTLFKLILGQEKVDAGTFEIGD
                     TVKLSYVSQDRFGTDDTRTVWDVISGGAETVKLGTRELSSRAYVSWFNFTGADQQKTV
                     NLLSGGERNRVHLALALKDPGNVLLIDEPTNDLDVNTIRALEDALINFAGCAVIVSHD
                     RWFLDRVATHILAFEGDSQVYWFNGNFSDYEEDKVRRLGDSALIPKRVQYKRFSK"
BASE COUNT          461 a          408 c          423 g          437 t
ORIGIN      
        1 cgatagatat tgcctcaatc aggcggattc ggtgctaaaa aattgtaaac cggtgagata
       61 ttgttgattt tcgggacagc acaataatga caaaaaccgg gagctatcag tatggataat
      121 aacccatata atcatattga caatcatatg gcatatgcta tgatgtcttg tggctggcaa
      181 ggcgacattt gagtgggcgg gttattggag aaagggcaag gtcatttatg aaaaagaaaa
      241 taaaatacac agatgagtcc ttgggtgata tcaaacttgt ttccgatcat tttcccaagc
      301 ctcacgaact ggttttcaag gaagataccg tcaaggtgac cattgaactc accaaaaaca
      361 gtgtggcatt tttcaaggaa caggccaaaa aacatcatac ccattatcaa gccatgatcc
      421 gcacccttct gaattattat tccaccatct gtggcaatga gagatgagac aatgcgtgtt
      481 catgagactt ttattgaatc tataggatga cgcctaatca ataattcccc tgactcacgt
      541 ggggatcatc gtcgagctgt ttgagaaaaa aacttttttg caggttctgg atgattccca
      601 gaacatcgtc cacaaaatcg ggggcgatgg agagttggag tttgggcaga gcttcatcca
      661 cagtagacac ctgaatcatg ttttcatacg cctcaagaat gtgcttgagg taatgaatat
      721 cggatttgtt gacttgatag aaaagataga acacgtagat cgtgaatcga caatcgtggg
      781 tcgcaaaata agagttgtct catcgggggt agggatccag aatgaatgca cacatggatc
      841 cctgctccgc agggatgaca aaatagattt cgatccacga tccgcgatcc acgctatttt
      901 gatggttatt tactaaatct cttatactgc acccgcttgg gaatcagggc gctgtcgccc
      961 aaacgacgca ctttgtcttc ttcgtagtcc gaaaaattgc cgttgaacca atacacctga
     1021 ctgtcaccct caaaggcaag aatgtgcgtg gccacccggt ccagaaacca gcggtcgtga
     1081 ctcacgatca cagcacagcc cgcaaaatta atcagcgcgt cttcaagggc ccggatggtg
     1141 ttgacatcca gatcatttgt tggttcgtcg atgagcagga cattgcccgg atccttgagg
     1201 gccagagcca gatgcactcg gttgcgctcg cctccggaca gcagattgac ggtcttttgc
     1261 tggtcggcgc ccgtaaaatt aaaccatgac acatacgctc gcgagctgag ttcgcgtgtt
     1321 cccagcttga cggtttcggc cccgccggaa atgacatccc acactgtacg tgtgtcatca
     1381 gtaccaaaac gatcctgtga gacgtagctg agcttgaccg tgtcgccgat ttcgaaggtg
     1441 cccgcgtcga ccttttcctg gcccaaaatg agtttgaaca gggtcgtctt gcccgcgccg
     1501 ttgggaccga tcacacccac gatcccgcct tgcgggaggt tgaattcaag attctcgaac
     1561 agcaatttat cgccaaacgc cttggagacc ttgttggcct caatcacttt ggcacccagg
     1621 cggggtccgg ggggaataaa aatttgcaaa tcgtcattgt actgttcgtt gctttgactc
     1681 aacagacttt cgtagttcgt gatgcgggct ttgctcttgc cgcgacgag
//
LOCUS       DATDUN010000301         1152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_388472, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000301 DATDUN010000000
VERSION     DATDUN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388472"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1152
                     /locus_tag="VJC18_04130"
     CDS             <1..>1152
                     /locus_tag="VJC18_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HLD44599.1"
                     /translation="ISLSAGIVVTRVTSDKKDSNLGRDVMMQLTSHPKVFVISSVLLA
                     GLAAVPGLPALPFLAISITCGAFAFSKLRKKQEKVKEIMQEPKEKKIKKAVEKHGDQL
                     PFILPSPISIEVGSAIIPFVDDSQDGGRFINELIPLLRHGLYYELGVNFPGIQVRGQT
                     IDMDPEAYIININEVPVARGKIMQNCILVGEPLEQLQLFNIEGKETIHPIDGSIVSWV
                     SSGFKEVCVQAGFRMWDISEYLILHLSYILRKHAHEFLGLQEVQTLLNELEKTHPALV
                     KELVPKVITVLQLAEIFQRLVQEEVAIRDLKNIFSTLAQWAEIERNTLILTEHIRAGL
                     KRYVTHKYAGHGNSLAVYLLDPTIEDLVKESIRTTEKGNYLALDPEITQ"
BASE COUNT          281 a          281 c          322 g          268 t
ORIGIN      
        1 catctctctg tccgcgggca ttgtggtcac acgcgtgacg agcgataaaa aagattccaa
       61 tctcggtcgt gatgtgatga tgcaactcac atcgcacccc aaggtgtttg tgatcagctc
      121 ggtcttgttg gcgggcctgg cggccgtccc cggattgccc gcgcttccct ttttggccat
      181 ttcgattacg tgcggggcgt tcgcgttttc gaagctccga aagaagcagg agaaggtcaa
      241 agagatcatg caggaaccca aagaaaagaa aattaaaaag gccgtcgaga aacatggcga
      301 tcaacttccc ttcattttgc ccagccccat ttccatcgaa gtgggttcag ccatcatccc
      361 gttcgtggat gacagtcagg acggggggcg ctttatcaat gagttgatcc cattgctcag
      421 gcatgggttg tattatgaac tgggtgtgaa ttttccgggc attcaggtgc gtgggcagac
      481 aatcgacatg gaccctgagg cttacatcat caacatcaac gaagtgcccg ttgccagagg
      541 caaaatcatg cagaactgca ttctggtggg ggaaccgctg gaacagttgc aactcttcaa
      601 cattgagggc aaggaaacga tccaccccat tgatggttcg atcgtgagtt gggtgagtag
      661 cgggttcaaa gaggtgtgtg tgcaggccgg attccgcatg tgggacatca gcgaatattt
      721 gattttgcat ttgtcgtaca ttttgcgcaa acacgcgcac gaatttttgg gattgcaaga
      781 ggtgcagaca ctgctgaacg aacttgaaaa aacccacccg gcgctggtca aggagctggt
      841 gcccaaggtg atcacggttc tgcagttggc cgaaattttt cagaggctgg tgcaggaaga
      901 agtggccatt cgtgatctca agaatatttt ttccactctg gcccagtggg ccgagattga
      961 acgcaatacg ctcattttga cagagcacat ccgggcggga ctcaaacgtt acgtgaccca
     1021 caaatacgct ggccatggca attcgctggc ggtgtatctg ctggatccga ccattgagga
     1081 tctggtcaag gaatccatcc gcaccacgga gaagggcaac tatctggcgc tcgatcccga
     1141 gatcacccaa ga
//
LOCUS       DATDUN010000302         1047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_139950, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000302 DATDUN010000000
VERSION     DATDUN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139950"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1047)
                     /locus_tag="VJC18_04135"
     CDS             complement(<1..>1047)
                     /locus_tag="VJC18_04135"
                     /inference="COORDINATES: protein motif:HMM:NF036810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HLD44600.1"
                     /translation="APSPINLKNSSPQILAVGPELKNTICLTDENRAFVSPHIGDLEN
                     VPTLDYFAEAVNHFQTILDLHPNILAYDLHPEYLSTKYVHEQKRVDLVTVGVQHHFAH
                     IASVMAEKQIDEPVIGLALDGTGYGTDGTIWGGEVLLCHTTHFRRAAHLRPLPMPGGS
                     LAIKENWRMAFAYLRSAFGDAWQTVSSECLKQISQENLLLLDQAITANINAPFTSSLG
                     RLFDAVASLLDIKHRNAFEGQAPMMLEAMATNGHPQFLLPFDITEQNHWAPVMQPTTS
                     LNIDVMGSLPEIKTQIIDYSATIRTLVTNWQKGVDRAELAMAFHQTIVQSLTQITQHI
                     SQATGITTVVLSGGS"
BASE COUNT          262 a          226 c          285 g          274 t
ORIGIN      
        1 ggacccgcca gagagtacaa cggttgtgat tccggtagct tgtgaaatat gctgtgtgat
       61 ttgtgtgagt gattgaacaa tggtctgatg aaaggccata gccaattcgg cccgatcaac
      121 ccccttttgc caatttgtaa caagggtcct gatcgttgct gaataatcaa tgatctgagt
      181 tttgatttct ggcaaggagc ccataacatc aatgtttaag gaggttgttg gttgcatgac
      241 aggggcccaa tgattttgct ctgtaatgtc aaatggcaga aggaactggg gatgaccgtt
      301 tgtcgccatg gcctccaaca tcatgggggc ctgtccttca aaggcgttgc ggtgtttgat
      361 gtccagaaga ctggccaccg cgtcaaagag acgacccagg gatgacgtga acggtgcgtt
      421 aatatttgcc gtaatggcct ggtccaacaa taagaggttt tcttgtgaaa tttgtttgag
      481 acactccgag gacacagttt gccaagcgtc gccaaaagct gaccgaaggt aagcaaaggc
      541 catgcgccaa ttttccttta tggccaaaga gccgcctggc atgggcaggg gtctgaggtg
      601 tgccgcacgt ctgaaatgag ttgtgtgaca caataatacc tcaccacccc aaattgtccc
      661 atcggtcccg tagccagtgc catcaagagc cagaccaatg acaggttcgt caatttgttt
      721 ttcggccatc actgacgcga tgtgagcaaa atgatgttgc acgcccacgg tgacgagatc
      781 aacgcgtttt tgttcgtgta cgtattttgt ggaaagatat tcagggtgca aatcgtaagc
      841 caatatattt gggtgcaggt cgaggatggt ttgaaaatga ttcacggcct cggcaaagta
      901 atccagagtg ggcacgtttt cgaggtcacc aatatgtgga gaaacaaagg ccctgttttc
      961 gtctgtcaga caaatcgtgt tttttaactc tggtcccacg gccagaatct gcggagaact
     1021 atttttgagg ttgatcggcg atggtgc
//
LOCUS       DATDUN010000303         4468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_553885, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000303 DATDUN010000000
VERSION     DATDUN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553885"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1499)
                     /locus_tag="VJC18_04140"
     CDS             complement(87..1499)
                     /locus_tag="VJC18_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44601.1"
                     /translation="MVHKLTEQKLNLLIAEGEGLSVEFKQKFSSKIAQDIVAFANSKG
                     GRLLLGVADDAKIVGEKLTNSMKAEIVHLAKTCEPSINVSTNQVGQVVVVDVPESDDK
                     PHMCSAGCFKRYDAVTQKLTPQDIRKILRTEAIGYFESRVNTNCQLKDLSLDKVRRFL
                     KTTGAGVTATQKNLPDILESHKLLQHKKITNAGIMMFAQDVSQYIMHCQIFLVAYKGT
                     EGINIYDRKDVRDDLLTQFDEALAFIEKHLNVRSEIRGKNRNDIYEIPLEALREAVTN
                     ALVHRDYNMSGTNVQVEIHADRVVVSNPGGLPEGLTLSLLGKKSIRRNEIIADLFHRM
                     NKMERVGSGIKRIKGLLKDAALPPPKFESDSFFTVTFERSKEFRTVQEEIFPGTTQET
                     TQETTQETTQETTQETTQETTQEKILAYLQSDPTVTRKELAEKIGITDDGIKYHLAKM
                     TGDGIVRHVGATKKGRWEIL"
     gene            complement(1664..2245)
                     /locus_tag="VJC18_04145"
     CDS             complement(1664..2245)
                     /locus_tag="VJC18_04145"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HLD44602.1"
                     /translation="MKTLTRCPWCGSDPIYIDYHDKEWGVPVHDDKKLFEFLVLESAQ
                     AGLSWLTILKRREGYRNAFADFDAEKVARFSEKRVAKLLQNKGIIRNRLKVTAAVSNA
                     KAFLKVQQKYGSFSDYSWRIVDGRTIVNKWERLDQLPVTSKESDAFSRDLKTRGFKFV
                     GSTIIYAHMQAVGMVNDHLTSCFRYKETGVCAS"
     gene            complement(2336..2602)
                     /locus_tag="VJC18_04150"
     CDS             complement(2336..2602)
                     /locus_tag="VJC18_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44603.1"
                     /translation="MTKALREKNLMLESRAIKEKLIKPRPLTVVEAMRELQEVRAIPV
                     QIKSTTMWVRTDIAGNVQKLFAAIGLKPPPKVLSLSKKIKNVVA"
     gene            complement(2774..3958)
                     /locus_tag="VJC18_04155"
     CDS             complement(2774..3958)
                     /locus_tag="VJC18_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44604.1"
                     /translation="MPHLRKRHVLPQLIKKLRFSPVVAMQGARQTGKSVLARDILPAT
                     LKQSFYVTLDQFSKREFVQERPGVFLEQNADFRPLIIDEAQKAPNIFDEIKHSVDQKR
                     RPCRFLLLGSTEFSKLSRIRESLTGRMSRLRLFPLTVSETLDQLSSTKNLFGSLTKRS
                     YCQRAQLIKYLKKGGMPALFSVRDDSEWTLAAQDWLELTVHRDCQQFPSVKIDGELCY
                     RILQQIAILEEPNAANLVKALKVNPRKLNTHLNVLQSLFVINALPPHSAGTGKTLFFL
                     LDTGLAGFFCASFERQLHTLALSEILAFSSYFETKPTQISYYRGSRQGLAHLVLESAE
                     GTVVIKILQSETFDKRELEILKSFRKKYSGVKPRLLAFGPERFNLKDLQIEVYPWENL
                     AL"
     gene            4091..>4468
                     /locus_tag="VJC18_04160"
     CDS             4091..>4468
                     /locus_tag="VJC18_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44605.1"
                     /translation="MITSPSDYMNEVKAKKQVVGTWGFNSNRDFTRTSTEETNVWFFE
                     QVFNFLRAFFGLVMPDNMEVITHNATKHIKRQNLDQQTFLDELMLVMKGLKEHIWTLR
                     LNLNIVGFIRAQGDPDNPVRVQIQ"
BASE COUNT         1139 a         1008 c         1052 g         1269 t
ORIGIN      
        1 gacttcgtct gtgatttcta tcagatcatt ttcagctttc aacacagaaa gaaattcgcg
       61 agtgcaggtg atggtttttt ttaaaattat aaaatctccc aacgaccctt tttggttgcg
      121 cctacatgtc ggacgatccc atcaccggtc atcttggcca aatggtattt aataccatca
      181 tcagtgatgc ctatcttctc ggctagttct tttcgagtca cggttggatc agactgcaga
      241 taggccagga ttttttcttg ggtagtttct tgggtagttt cttgggtagt ttcttgggta
      301 gtttcttggg tagtttcttg ggtagtttct tgggtagtgc caggaaatat ttcttcttga
      361 acagttctga attccttcga ccgctcgaag gttaccgtga aaaaagaatc gctctcgaat
      421 tttggaggtg ggagtgcggc atcttttaaa aggcctttga tgcgtttaat accagacccc
      481 acgcgttcca tcttgttcat tctgtgaaag aggtcagcga tgatttcatt tcgacgaatg
      541 gattttttcc ccaaaagtga aagggttaat ccctcgggca aacctcctgg gttggatacc
      601 accacacgat ccgcatgaat ttcgacctga acgttggtgc ccgacatatt gtaatcccgg
      661 tgaaccaagg cgttcgtgac ggcttctcgc agagcctcca gaggaatttc ataaatgtcg
      721 ttgcgatttt taccacgaat ttcgctacgg acattcaaat gtttttcaat gaaggccagg
      781 gcctcatcaa actgggtgag caaatcatcg cgcacgtctt ttctgtcata gatattaata
      841 ccctcagttc ccttataggc gaccaaaaaa atctggcaat gcatgatata ttgactcaca
      901 tcctgagcaa acatcatgat acccgcattg gtaatttttt tgtgctgtaa aagtttgtga
      961 ctctcaagga tatctggcaa gtttttttga gttgcggtaa caccggcacc agttgtttta
     1021 agaaaccgtc tcaccttatc gagtgacaaa tcttttaact gacaatttgt gttcactctg
     1081 gattcaaaat aaccaatcgc ctctgtccgc aaaattttac ggatgtcctg cggtgtgagt
     1141 ttttgtgtga cagcatcgta gcgtttaaaa caaccggcgg aacacatgtg tggtttgtca
     1201 tcgctttcag gaacatcaac cacaacaact tgaccaacct ggtttgtgga aacattgata
     1261 gaaggctcac aagtcttggc caaatgaacg atttcggctt tcattgaatt ggtgagtttt
     1321 tcgccaacaa ttttggcatc atcagccacg cccaatagaa ggcggcctcc tttcgaattg
     1381 gcaaaggcca caatatcttg cgcgattttt gaagagaatt tttgtttgaa ttcaacggac
     1441 agaccttctc cttcagctat caggaggttg agcttttgtt ctgtaagttt atggaccata
     1501 acttgagatt acaactattc cgttaacacg atcatttttt tttaaccatc gcattttttc
     1561 agtgtgaaca aagcggcttg tcgtgtgctg gttgggggtt gcttgagcca catgcgggct
     1621 ggcttagtcc agcgaaaacg gggggtcaat ggatttgcca ttatcaagag gcacagacgc
     1681 cagtttcttt gtaacgaaaa cagctcgtga gatgatcgtt gaccatccca accgcctgca
     1741 tgtgcgcgta gatgattgtt gaccccacaa acttgaaacc gcgcgttttc aaatctctgc
     1801 tgaaagcatc ggattctttt gatgtgacag gcaactgatc caagcgttcc cacttgttga
     1861 cgattgtgcg gccatcaaca atgcgccaac tgtagtcgct gaatgatcca tatttttgct
     1921 gcaccttcaa aaacgccttg gcattggaca ctgccgccgt caccttgagg cgattgcgga
     1981 taatgccttt attctgcaag agttttgcca cacgcttttc cgaaaaacgc gccacctttt
     2041 ctgcgtcaaa atctgcaaag gcattgcgat agccttcgcg gcgtttgaga atggtcagcc
     2101 agcttagccc tgcctgcgct gattccagca ccagaaactc aaaaagtttt ttatcgtcat
     2161 gaacgggtac gccccactct ttgtcgtgat agtcgatgta aatcggatca cttccgcacc
     2221 aggggcaacg tgtgagcgtt ttcataggtg atccgtttac gtccaaaggt cccgacgtcg
     2281 acaccagaaa ataaaaagct tttctttttc aggcagttag agacgagacg ctgccttatg
     2341 ccactacatt tttgattttt ttggaaagac tcaaaacttt gggcggtggt tttagtccga
     2401 tggcagcgaa gagtttttgt acgttaccag cgatgtcggt tctcacccac attgtggtgg
     2461 attttatttg aacggggatc gctcgcactt cttgtagttc tcgcatggcc tcaacaacag
     2521 tcagaggtct gggtttgatg agtttctctt tgatggcacg actttccaac atcagattct
     2581 tttctctcaa cgctttcgtc atcagtgact cacaaagata agccagaaaa cacattgtca
     2641 ggtgtccgat gatgttatga gcttttttct gatcttttgc aaaatatctt ctctggcttt
     2701 tatgtcgcgt tccgcgcggg cgcgcggatt gaaaccagtt tgacacagca caagcccttt
     2761 ctctggggaa aaatcatagg gccaagtttt cccagggata gacttctatt tgcaggtcct
     2821 tcagattgaa tctttccggg ccgaaagcca aaagtcgcgg cttcacacca gaatattttt
     2881 ttctaaagga tttcagaatt tcaagctcgc gcttgtcgaa ggtctcgctt tgaagaattt
     2941 ttataaccac agttccctcg gcagattcca agaccaagtg tgccagcccc tgtctgctcc
     3001 ctctgtaata agagatttgt gttggcttgg tctcgaaata cgaagagaag gccaaaattt
     3061 cagagagggc caaggtgtgc aactgacgtt caaaggaggc acaaaaaaaa ccggctaacc
     3121 cagtatccaa caaaaaaaag agggtctttc cggtaccggc agaatgggga ggcaacgcat
     3181 tgatgacgaa caaagactgg agaacattaa gatgagtatt aagttttcgc ggattaacct
     3241 tgagcgcttt tacaagattc gcggcattgg gctcttccaa aatagcgatc tgttgcagaa
     3301 tgcggtagca aagttcgcca tcgatcttga cagagggaaa ttgctgacaa tcgcgatgca
     3361 ctgtcaattc aagccaatcc tgtgcggcca gggtccattc ggaatcatct ctgacagaaa
     3421 aaagtgcggg catccctccc tttttgaggt atttgatgag ttgggctcgt tggcaataag
     3481 accgttttgt gagagatccg aacaggtttt ttgtcgagga aagttggtct agggtctcgg
     3541 acacggtaag ggggaacaat cgcagacgac tcattctacc ggttaaggat tccctgattc
     3601 gagaaagttt cgaaaattcc gtagaaccca aaagaagaaa gcggcagggc cttcgttttt
     3661 gatcaacgga gtgcttgatt tcgtcgaaaa tattgggggc tttttgtgcc tcgtcaataa
     3721 taagaggacg aaaatcagcg ttttgttcca aaaaaacgcc cgggcgttcc tggacgaatt
     3781 cacgtttgga aaattggtcc aaggtaacgt aaaaggactg tttcagggtt gcgggcagga
     3841 tatcgcgtgc caaaacgctc tttcccgtct gtcgagcgcc ttgcatggcg accaccgggc
     3901 tgaagcgaag tttttttatc agttgtggca ggacgtgacg ttttctgagg tggggcatgt
     3961 tccattttaa cccatccaat tcacgactgt caacgtccat aatttgatgg gttaaaaaca
     4021 aggtgacgat ccctaaaacg gacaatcatt accatttgat tattccctgg caaacgaata
     4081 ggtgttttcc atgatcacca gtccttccga ttacatgaac gaggtcaagg ccaagaaaca
     4141 ggtggtgggg acctgggggt tcaattcgaa cagggatttt acccgcacga gcaccgaaga
     4201 aaccaacgtc tggttttttg aacaggtctt caattttttg cgcgcttttt ttggtctggt
     4261 gatgcccgat aacatggaag tcatcaccca caacgcgacc aaacacatca aacgacagaa
     4321 tctcgaccag caaacatttc tggatgagct catgctggtg atgaaggggc tcaaggaaca
     4381 catctggacg ctcaggctca atctgaacat tgtcgggttc atccgggcac agggcgatcc
     4441 cgacaatcct gtgcgagtgc agatccag
//
LOCUS       DATDUN010000304         1874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_180674, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000304 DATDUN010000000
VERSION     DATDUN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180674"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VJC18_04165"
     CDS             complement(<1..618)
                     /locus_tag="VJC18_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLD44606.1"
                     /translation="MKNHDDQKSIVLCLILLFSFAFFACNRPSVYEIPKSATNLAPDQ
                     ALASGQFRLIVDASMIQIRVVKDDHIPVRGTFNLSEGQLVLKSIENAIGSVLLDITSW
                     DSGLSERDAIITKLLLQSEQPGFEKASLDLGVLPKQAVEALIKTKRVDDVTLDAVLLF
                     KGNRVSLPLKVKMFFDERDRFVVQSVSPSMVTLSALGLKDLLPEYI"
     gene            complement(615..1634)
                     /gene="queG"
                     /locus_tag="VJC18_04170"
     CDS             complement(615..1634)
                     /gene="queG"
                     /locus_tag="VJC18_04170"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HLD44607.1"
                     /translation="MTADLKRQIQFLANDHGFDACGFTNVLEPEHRDYFLSWLKAGQH
                     ADMRWMERGCDKRLNPRLLMEGATSAIVLLASYQHSANQSDYRLARYAWGRDYHLWLR
                     QKADELALGIKKRFCPDLLYRVFVDTGPILERDLACKAGLGWIGKNTCLINPRFGSFV
                     FIAVILTNLDLSADETIKDHCGRCDRCVSACPTQALKPYQLNASLCLAYHNIEKRGER
                     EPVIRDKMGDRLVGCDLCQEVCPHNKSKPETNIVEWLATFDQYQLGSLGDLLSLSEHD
                     YELKTRDSALSRLKYFDFMRNVFIVIANAARTDLLNEVCAWQLRHPTMLMSECVACAQ
                     KLGVK"
     gene            complement(1624..>1874)
                     /locus_tag="VJC18_04175"
     CDS             complement(1624..>1874)
                     /locus_tag="VJC18_04175"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HLD44608.1"
                     /translation="FTILNQYQTDRFLLNHLDLYRMQTFDELEHIDLLSCFEDPGAIT
                     FVEWADKFDELRSEYTKLVHFEYIDGEALKRKITCHDC"
BASE COUNT          523 a          493 c          419 g          439 t
ORIGIN      
        1 aatgtattcg ggcaacaggt ccttcagtcc caatgccgag agcgtcacca ttgatggtga
       61 gactgattgg accacaaacc ggtcacgttc atcaaagaac atttttactt tcagaggcaa
      121 cgacacacga ttgcccttaa ataacaacac ggcatcaagg gtcacatcgt caaccctttt
      181 ggtcttgatc agagcttcaa cggcctgctt tggcaaaacg cccaaatcaa gactggcctt
      241 ttcaaaaccc ggttgttcac tctgcaacaa gagcttggtg atgatcgcat cacgctcgct
      301 caaaccactg tcccacgaag tgatgtcaag cagaaccgac ccgatggcgt tctcgataga
      361 tttgagcacc aactgccctt cactgagatt gaaagtaccc cgaaccggga tgtgatcgtc
      421 cttgaccacg cggatctgga tcatggaggc gtccacaatc aacctgaatt gaccggacgc
      481 caaggcctga tccggtgcca aattcgtagc cgatttgggg atttcgtata cactgggacg
      541 gttacaggca aaaaaagcaa aactgaagag cagaatgaga cataagacaa ttgacttttg
      601 atcatcatgg tttttcattt cacccccagt ttttgcgcgc atgccacaca ctcggacatc
      661 agcattgtgg ggtggcgcaa ttgccaagca cacacttcat tcaacaaatc tgtccgcgcc
      721 gcgttggcga tcacaataaa cacattacgc atgaaatcaa aatatttcag acgcgacagg
      781 gccgaatcac gtgtcttgag ttcataatca tgttcactca aagaaagcaa atcacccaaa
      841 ctgcccagct gatattgatc gaatgtggcc aaccactcca caatgttggt ctcaggttta
      901 gacttgttgt gcgggcaaac ctcctgacac agatcacaac ccaccagacg atcacccatt
      961 ttgtccctga tgaccggttc acgctctccc cttttttcga tgttgtgata tgccagacac
     1021 aacgaagcat tcagttgata gggtttgagg gcctgcgtgg gacacgcact cacgcaacga
     1081 tcacaacgac cacaatgatc cttgatggtc tcatctgcac tcaagtcaag attggtcaaa
     1141 atcacggcta taaaaacaaa agacccaaat ctgggattga tgagacaggt atttttgcca
     1201 atccagccca gcccagcctt gcaggccaaa tccctttcca aaatcggccc cgtatccaca
     1261 aagacacggt aaagcaggtc gggacaaaac ctctttttga taccgagggc caactcgtca
     1321 gccttttgtc tgagccacag gtgataatca cggccccaag cgtaacgtgc cagacgatag
     1381 tcactttgat tcgctgaatg ttgataagaa gccaacaaca caatcgccga tgtggcaccc
     1441 tccatgagga ggcgaggatt aaggcgttta tcacaaccac gttccatcca gcgcatgtcg
     1501 gcgtgttgcc ctgccttgag ccacgacaaa aaatagtctc tgtgttctgg ctccagaaca
     1561 tttgtgaacc cgcaggcatc gaacccatgg tcattggcaa ggaattgaat ctgtcgtttg
     1621 aggtcagcag tcatggcaag tgattttgcg tttcaaggct tcaccatcga tatactcaaa
     1681 atgaaccagc ttggtgtatt cgctcctgag ttcatcaaat ttatcggccc actccacaaa
     1741 ggtgatggca cctgggtcct cgaaacacga cagcaggtcg atgtgttcga gttcatcgaa
     1801 ggtttgcata cggtataaat caaggtgatt cagtaaaaac cggtcggtct ggtactgatt
     1861 gagaatagta aacg
//
LOCUS       DATDUN010000305         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_180721, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000305 DATDUN010000000
VERSION     DATDUN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180721"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1515
                     /locus_tag="VJC18_04180"
     CDS             61..1515
                     /locus_tag="VJC18_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD44609.1"
                     /translation="MKILIVSPNREAFVHRVAPIGPLYLVAALEERGHTVRFVDGMFL
                     SDPFEAFAQAVRDFQPDLVGLAIRNIDTLLAKDVYHMPEFKRYIEILRQFSKAPVVAG
                     GPGFSLMPDEIMRSFDLDFGIAGEADLALPALAESLNNKTSYASLPGLLYRDQGKLII
                     NKPEQIEDLDAIPFQAVAHIDAKSYGRYRGNLAVFTRKACPMKCIYCPEAALHGNRVR
                     LRSAKRVVDELEYIVKTTGVLDFDFADTTFNVPREHAVAVCEEIIRRGLKIRFEVELS
                     PIGQNDESVRLLKAAGCIGVDLTAESGSDRMLDTLRRGFTADLTLKAGALYKRHGIPY
                     TAGFLLGGPGENRQSVEDSIHLAKALPGMTSAYFTVGIRLFKNTELVRLALEEGVIKS
                     TDELIKPVFYLSEQFDTACAHSLLTACHRSLRLYVSDLFYTPMMRKFFGTADRRNVRP
                     IWKFGTLPKLLEWGLRLGRPGLRWDQGKRCYVAS"
     gene            1708..>2464
                     /locus_tag="VJC18_04185"
     CDS             1708..>2464
                     /locus_tag="VJC18_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLD44610.1"
                     /translation="MTTAIETFFDDLIKEWGSDRVTRDPSVWAPLLTNTLGVHRTLTG
                     VVYPESAEDVCQVVKMARQHKVPLYPISCGQNVGYGQRLPVRDGSVLVDLGRMNKIKE
                     FDSIQGSVVVEPGVTQGQLYQFLEDQKAKFWLDATGAGLRASIIGNSLEGGFGHTPKG
                     DHRRFWTDVALVLGNGQVLKTGTFPGLGPDLAGLFVQSNFGIITSARVELMPKPERFV
                     SFIVSVKGDEQLGSLVDAMRQLRQAGHLQSLVHI"
BASE COUNT          528 a          584 c          727 g          625 t
ORIGIN      
        1 tgccttgcat gccggtatgg agcgcctgtt gtcttcggac aaacgggctg tgaagtgatc
       61 atgaaaatac tcattgtttc ccccaatcgc gaggcgtttg tgcatcgagt ggcgcccatc
      121 ggtccactgt atctggtggc ggctctggag gaacgcgggc acacggtacg ttttgttgat
      181 ggcatgtttt tgtccgatcc gtttgaagcc tttgcccagg ctgttcgcga ttttcaaccc
      241 gacctggtgg ggttggccat tcgcaacatc gataccctgc tcgccaagga tgtgtatcat
      301 atgcctgagt tcaagaggta cattgaaatc ctgcgccagt tttcgaaagc ccctgtggtg
      361 gctggcggac cgggattcag tctgatgccg gatgagatca tgcgttcttt tgacctcgat
      421 tttggcattg cgggtgaagc ggaccttgcc ctgcctgcac tggcggagag tctcaataat
      481 aaaacatcgt acgcatccct tcccggactt ttgtatcgtg atcagggaaa actcatcatc
      541 aacaaaccag aacagattga agacctcgat gccattccct ttcaggcggt ggctcatatc
      601 gacgccaaaa gttatggccg gtatcgcggt aatctggcag tctttacgcg caaggcctgt
      661 ccgatgaaat gcatttactg tccggaggcc gccctgcatg gcaaccgggt gcgcttgcgt
      721 tcggccaaac gcgtggtcga tgaactcgaa tacatcgtga agaccacggg ggtgctggat
      781 tttgatttcg cggataccac cttcaatgtc ccgcgggaac acgccgtggc tgtgtgcgag
      841 gaaatcatca ggcgtgggct caagattcgt tttgaggttg agctgagccc catcggtcag
      901 aatgatgagt ccgtgcgtct gctcaaggcc gcgggctgta ttggcgtgga tctgaccgct
      961 gaatccggca gtgacaggat gctggacaca ctgcgtcgcg gttttacggc tgatcttacc
     1021 ctcaaagcag gggcgttgta caaacgacat ggtattccgt acacagcggg ttttttgttg
     1081 ggtggtcccg gtgaaaacag acaaagcgtt gaggatagta tccacctggc caaggccctg
     1141 ccagggatga cctcggccta ttttactgtg ggtatccgcc tattcaagaa caccgaattg
     1201 gtgcgtttgg ctctggaaga aggtgtcatc aaatccacag atgagctcat caagcccgtc
     1261 ttttatttga gcgagcagtt tgataccgcc tgtgcccaca gcttgcttac ggcttgtcac
     1321 agaagcctgc gcctgtatgt cagtgattta ttttacaccc ccatgatgcg caaatttttt
     1381 ggaacggcgg atcggcgtaa cgtcagaccg atatggaagt ttggtacttt gccaaaatta
     1441 ttggaatggg gcctgcgttt ggggagaccc ggtttaaggt gggatcaggg caagcggtgt
     1501 tatgttgcct cttagtgcct attcgaaaac ctcctgaagg ggcggtggat ggcaatgtgg
     1561 catttttcct gcaccctttc agggtgcaat attaccggcg cctgttttcc gtaggctaaa
     1621 gcctacggct gtattcccac atcccttcgg gacgacagtt atccacaggt tttcgaatag
     1681 gcacttaaag agcatgggga ggaatcaatg accacagcga ttgagacttt ttttgatgac
     1741 ctgatcaaag aatggggcag tgatcgcgtc acgcgggacc cgtcggtatg ggcaccgttg
     1801 ctgaccaaca ctctgggcgt tcatcggacc ctgacaggtg tggtgtatcc cgaatcagca
     1861 gaagacgttt gccaagtggt aaagatggcc agacagcaca aggttcccct ttatccgatc
     1921 agttgtgggc agaatgtggg gtatggtcag aggcttcctg tccgcgatgg ttctgtattg
     1981 gtggatttgg ggcgcatgaa caaaataaaa gagttcgaca gcattcaggg gtcagtggtg
     2041 gtggagcccg gggtgaccca ggggcagttg taccaatttt tggaagatca aaaggccaag
     2101 ttctggttgg acgcaaccgg ggcagggctt agggccagca tcattggcaa ttctttggaa
     2161 ggcgggtttg ggcatacgcc caaaggggat caccgtcgtt tttggaccga cgttgcactg
     2221 gttttgggaa acggtcaggt tcttaaaacg gggacttttc cgggactggg accagatctg
     2281 gcgggacttt tcgtgcagtc aaactttggc attatcacga gcgcgagggt tgaactcatg
     2341 cccaagcccg aacggtttgt gtcgttcatt gtcagcgtca aaggggatga acaattgggt
     2401 tctctggttg atgccatgcg ccagctgaga caggcagggc atttgcagag tttggttcac
     2461 atcg
//
LOCUS       DATDUN010000306         1188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_513311, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000306 DATDUN010000000
VERSION     DATDUN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513311"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /gene="rpsB"
                     /locus_tag="VJC18_04190"
     CDS             complement(<1..693)
                     /gene="rpsB"
                     /locus_tag="VJC18_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HLD44611.1"
                     /translation="MAQVQIPEMMEVGAHFGHQTKRWNPKMRPYIYGDRSGVHIIDLG
                     QTKQLADAALAFIKSVVASGERVLFVGTKTQARQIIREQAERVGMHYVNNRWMGGTLT
                     NFNTIKKSIDRLIDLQTRRQNEDFKGYTKKELLDVDRTVVKLEASLGGIKSLDRTPGA
                     VFVVDPTHERIAVREARKLGIPIVAMVDTNCDPDPIDYIIPSNDDALGAIIYFVNKIT
                     EACQEGLQDGEKI"
     gene            1006..>1188
                     /locus_tag="VJC18_04195"
     CDS             1006..>1188
                     /locus_tag="VJC18_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44612.1"
                     /translation="MYKFHISKLGLFCCLFVLLAVLSTTSFADDADEDYQAYESTKYA
                     SSKWDAQVKQGMEAYHS"
BASE COUNT          302 a          294 c          252 g          340 t
ORIGIN      
        1 aattttttcg ccatcctgca aaccttcctg acaagcttcg gtgattttat ttacgaagta
       61 aatgatggcg cccaaggcat catcgttcga agggatgatg taatcgatgg gatctgggtc
      121 acagttcgta tcaaccatcg ccacaatggg aatgcccagt ttgcgcgctt cacgcacagc
      181 aatgcgttca tgtgttggat cgaccacaaa cacagcacca ggtgttctat ccaaagactt
      241 gatccctccc aaggaggcct cgagtttgac aactgttctg tcgacatcga gcaattcttt
      301 tttggtatac cctttgaaat cttcgttctg acgacgtgtc tggagatcaa tcaagcgatc
      361 gatgctcttt ttgatcgtgt tgaagttggt caacgtgcca cccatccaac ggttattcac
      421 gtaatgcatg ccaacacgct cagcctgctc gcgaataatt tggcgagcct gcgtttttgt
      481 tcccacaaaa agcacgcgct caccactcgc caccacagat ttgataaacg ccaatgcggc
      541 gtccgccaat tgtttggtct gccctaaatc aataatgtga acccctgaac gatccccgta
      601 aatatagggg cgcattttgg gattccacct ttttgtttga tgcccaaaat gagcaccgac
      661 ttccatcatt tctggaattt gaacttgagc catatttctc ctttggttta cccagttacg
      721 cgcgacaaat gaagtcactc atgaccggct tccaactcag ttgctttttt ggactagaaa
      781 agtcttaacc ccccataatc cttgatagga caaccaggag gacaactggg ttgtgttttt
      841 ttgttgattg atctactaat actgccgtcc caaaaaaaca ggacagcccc ttgtaatcaa
      901 ggccacggct cgctaacaca cggctgtcct taacgcaaca tgaaacatca gcaggattcc
      961 ccttgattgt agtgccaaac attgtatgat aacattatgt taatattgta taaatttcac
     1021 atttcgaagt tggggttgtt ttgttgtctc tttgtgctgt tggcagttct gtcaaccaca
     1081 tcgtttgctg atgatgctga cgaggattat caggcctatg aatctacaaa atacgcgtca
     1141 tccaagtggg atgctcaggt caaacagggt atggaggcct atcactcg
//
LOCUS       DATDUN010000307         1218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_430110, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000307 DATDUN010000000
VERSION     DATDUN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430110"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..737)
                     /gene="gmk"
                     /locus_tag="VJC18_04200"
     CDS             complement(174..737)
                     /gene="gmk"
                     /locus_tag="VJC18_04200"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLD44613.1"
                     /translation="MTQAPHRPPFVVISGPSGSGKTTICREIAKHFDWYYSVSHTTRK
                     IRKGEAHGQDYFFVSDDDFQKMVGRNEFLEWAQVYQNRYGTSQQIIEDKLSSGQGVVV
                     DVDTQGAQIIKQKMPEAILIFIKPPDVEELKKRLVSRGRDPETDIEKRLMQTQQELAR
                     MSAYDHVVVNDKLDRAVQEIISILNDK"
     gene            complement(718..>1218)
                     /locus_tag="VJC18_04205"
     CDS             complement(718..>1218)
                     /locus_tag="VJC18_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44614.1"
                     /translation="CSGWALSTPASILCILGLIVFIRWVPRVQSQRNIFGLGLEILIL
                     LGLIVTTISSYLFILNKAFFSNNPLTYETFCSTRSPLFGLNQVANFCWEVALTQPEQT
                     LKSPYLLTVPTLAIRIVYFVWAIVVGFFLFGELKNLLPGLKHYFGLGTKPLFKRKPKK
                     NDASAT"
BASE COUNT          320 a          293 c          294 g          311 t
ORIGIN      
        1 tgtgtttacg tgcgcgtgtt gaaattcaac ttggatatgt cattcctgcg aacgcaggaa
       61 tccagaatta ctgtaaatat ggatccctgc atgcgcaggg atgacaaaag ccatcttcct
      121 ttcattgtcc aaatacccaa cgcacgcacg ccaacacgta cgcacgaatt taatcacttg
      181 tcattcaata tcgaaataat ttcctgcaca gcgcgatcga gtttgtcgtt aacgaccacg
      241 tgatcatagg cactcattct agcaagttcc tgctgggtct gcatcaatct tttttcgatg
      301 tcggtttcgg ggtcccgccc ccgggaaaca agccgtttct ttagttcctc gacatcggga
      361 ggtttgatga agatcagaat ggcttcgggc atcttttgtt tgatgatttg tgccccctgt
      421 gtgtcgacat ccaccaccac accctgacca ctggaaagtt tgtcttcgat gatttgctga
      481 gaggtgccgt aacggttctg gtacacctgc gcccattcca gaaactcgtt gcgcccaacc
      541 attttctgaa agtcatcatc ggatacaaaa aaatagtctt gcccgtgggc ctcgcccttg
      601 cggattttgc gggtcgtgtg cgaaacggaa taataccaat cgaaatgttt cgcaatttcg
      661 cgacaaatcg ttgtcttgcc cgatccgctc ggtcccgaaa tgactacaaa aggaggtcta
      721 tgtggcgctt gcgtcatttt tttttggttt ccttttgaac aggggcttgg ttcccagacc
      781 gaaatagtgc ttcagtccgg gcaaaaggtt tttcagttcc ccaaacagga aaaaacccac
      841 cacaatggcc cagacaaagt acacgatgcg tatggcgagt gtgggcacag tcaacaaata
      901 gggcgattta agggtttgtt ctggttgtgt caaggccact tcccaacaaa aattggccac
      961 ttgattcaga ccaaacaagg gggatcgagt cgagcaaaac gtctcatagg tcaaggggtt
     1021 gttcgaaaaa aaggctttgt tgagaatgaa aaggtatgaa ctgatcgtag tcacaatcag
     1081 gccaagcagt atcaatattt ccagaccgag accaaaaatg ttgcgttggg attgcacgcg
     1141 ggggacccaa cgaataaaaa cgatcagccc cagaatgcag agaatggatg ctggtgtgct
     1201 cagggcccag ccggaaca
//
LOCUS       DATDUN010000308         1298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_513386, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000308 DATDUN010000000
VERSION     DATDUN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513386"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..615)
                     /gene="arfB"
                     /locus_tag="VJC18_04210"
     CDS             complement(190..615)
                     /gene="arfB"
                     /locus_tag="VJC18_04210"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HLD44615.1"
                     /translation="MIQITRTITIDEGEIHLKFIRAPGPGGQNVNKVATAVQLRFDVV
                     KSPSLPDDVRKRLIHLAGRRITEEGVLIIEARRFRTQEGNRQDAISRLTELIRKAAEK
                     PKPRRKTKPTFASKRRRLETKHRKGEAKRMRRSAPHLED"
     gene            complement(914..>1298)
                     /locus_tag="VJC18_04215"
     CDS             complement(914..>1298)
                     /locus_tag="VJC18_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLD44616.1"
                     /translation="VHQIEPLIASTHIPPAFVGLIILPLVGNVAEHSVAITSATKNKI
                     NLSLAIAAESSTQIALFVSPICVLIAGLFGQNFNLIFNKIELITLGMSIGGVWLVVHD
                     NKSNWWEGFLLLSFYFIIAITAYFL"
BASE COUNT          325 a          308 c          315 g          350 t
ORIGIN      
        1 ccgcacggac atcccctgat ttccaaagga atatcataca ggtaatacct gcacataaaa
       61 tcgctcgttt caatttttga ttcttcatat aatttatgac ccttcaacaa catgcttgcc
      121 acagaggtca cagagatcac agagagtttt tcctctgtgc cttctgtgta ctctgtggct
      181 atattgtggt taatcttcta aatgcggagc tgaccgtcgc atgcgttttg cctcgccctt
      241 gcgatgcttg gtttccagcc tgcgtctctt tgatgcaaag gtaggttttg ttttgcggcg
      301 gggcttgggc ttttcggctg ccttacggat tagttcagtc agacggctga ttgcatcctg
      361 ccgattacct tcctgtgtac ggaaacgcct ggcttcgata atcaatacac cttcttcggt
      421 aatccgcctg ccagccaggt gaatcaaccg cttgcgaaca tcatcgggca gtgacggtga
      481 tttgaccaca tcaaaacgga gctgcacagc ggtggcgacc ttgttgacat tctgcccgcc
      541 aggccccgga gctcggatga acttcaggtg aatttcgccc tcgtcaattg tgatggtgcg
      601 tgtgatttga atcatgcctt tgcctttttg tgcttactaa aaattacttt agcatcaatc
      661 aaaaaagtgc caagtgacgt gtggcgagtg acgggtataa accgcttgtt acctgacact
      721 cgacacttgt cattatctct atgtgcttgc taaaacttgt ctcatgaaaa ttgggaaaaa
      781 cctttacagg gtaaaccctt ataaaatggc atgaaaatat gccaccttcc ctttgtccct
      841 cccatcaagg gagggaaagc ttcctcgccc cttgtgggag aggattaagg tgaggggatt
      901 ttcgtgtcaa atactataaa aaataggcgg tgatggcgat gatgaagtaa aacgacaaca
      961 gcaaaaaccc ttcccaccag ttgctcttat tatcgtggac gaccagccac acgccgccaa
     1021 tggacatgcc cagggtgatc aattcgatct tgttaaaaat caggttgaaa ttctggccaa
     1081 aaagacccgc gataaggacg caaatgggcg aaacaaagag ggcaatctgt gtgctgctct
     1141 ctgcggcgat ggcaaggctc agattgatct tgttcttcgt agcgctggtg atagctacgg
     1201 aatgttcggc tacgttgccg actaaaggca agatgattaa gcccacaaag gcaggtggaa
     1261 tgtgcgtgga tgcaataaga ggctcgatct ggtgcacc
//
LOCUS       DATDUN010000309         1073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_305542, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000309 DATDUN010000000
VERSION     DATDUN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305542"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..78)
                     /locus_tag="VJC18_04220"
     CDS             complement(<1..78)
                     /locus_tag="VJC18_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HLD44617.1"
                     /translation="MNDRIRVPEIRVIGSQGDQLGVMTPF"
     gene            complement(187..>1073)
                     /gene="thrS"
                     /locus_tag="VJC18_04225"
     CDS             complement(187..>1073)
                     /gene="thrS"
                     /locus_tag="VJC18_04225"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HLD44618.1"
                     /translation="DLPCRWAELGTVYRYERSGVLHGLMRVRGFTQDDAHIICTQEQM
                     ADEILCVLKFSLHMLRSFGFVNYELYLATRPQEKYVGEIQLWDDAQNALRRALEAEGV
                     SYQVDEGGGAFYGPKIDIKIEDAIGRQWQCSTIQFDFNLPERFDMTYIGSDGAKHRPY
                     MIHRALLGSLERFFGILIEHYAGHFPLWLAPVQAVVINVTTDQLAYAREISEKLNQAG
                     FRVALSDHNEKLGYKIREAQLQKVPYMIVVGKNEVEAGTLAIRQSQGGDLGSFTFEDF
                     VKRLQDENIAGKVVTGHA"
BASE COUNT          248 a          328 c          248 g          249 t
ORIGIN      
        1 aaacggggtc atgaccccca attgatctcc ctgtgaaccg atcacacgaa tctcgggaac
       61 cctgatgcga tcattcatgc gtggcccatc aatcctgggg cgaaatggcc ttctcccccc
      121 tcggggtggt ctgctgttta gaataataac ctcctatttt gcggtgttca gtcgattggt
      181 ccgccatcaa gcatgaccag tcacaacctt tcccgcaatg ttttcatctt gaagcctttt
      241 aacaaaatcc tcgaacgtga acgaacccaa atcgcctccc tggctctgtc tgatggcgag
      301 cgtgcccgcc tcaacttcgt tctttccgac cacaatcata tagggcacct tctgcagttg
      361 cgcctcgcga atcttgtacc cgagtttttc gttgtgatca gataaagcca cccgaaaacc
      421 cgcctgattg agtttttcgc tgatctctct ggcataagca agctgatcag tcgtgacatt
      481 gataaccaca gcctgcaccg gcgccaacca caagggaaaa tgccccgcgt aatgttcaat
      541 gagaatgcca aaaaatctct ccagcgaacc cagcaaagcc ctgtgaatca tgtagggtct
      601 gtgtttggcg ccatcgctcc cgatgtaggt catgtcgaat ctctcgggga gattgaaatc
      661 gaactggatg gtggaacatt gccattggcg cccgatcgcg tcctcaatct tgatgtcgat
      721 cttgggcccg taaaaagcgc cgccgccctc atcgacctgg tacgacaccc cctcggcctc
      781 caaggccctg cgcaaggcat tttgcgcgtc gtcccacaat tggatttcgc ccacgtactt
      841 ctcctggggt ctggtcgcca gatacagttc ataattcaca aaaccgaaag acctgagcat
      901 gtgcaggctg aatttcaaca cgcacaggat ctcgtcggcc atctgttcct gcgtgcaaat
      961 gatgtgcgcg tcgtcctgcg tgaacccgcg caccctcatg agcccatgca atacaccgga
     1021 acgctcgtaa cgatagacgg tacccaactc agcccaacga cagggcaggt cac
//
LOCUS       DATDUN010000310         5715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_17713, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000310 DATDUN010000000
VERSION     DATDUN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17713"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1426)
                     /gene="nusA"
                     /locus_tag="VJC18_04230"
     CDS             complement(89..1426)
                     /gene="nusA"
                     /locus_tag="VJC18_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HLD44619.1"
                     /translation="MIVDLNHVLEQIQKEKGIAKETLIEAIEAAMMSAARKKLGFYGD
                     LEAQYNPEMGEVELFQYKTVAEEVEDDQLEMDLITARKLDGEAMVGDSFGVKIDQKDL
                     GRIAAQTAKQVIIQKLREAEKDVIVNEYESKLGQILSGVVRRFEKGNLVIDLNKTEAV
                     IYRRELVEGETFKTGDRIQALLIEIDTRARAQMLILSRRDPQFVKSLFEMEVPEISEG
                     IVEIKNVARDPGVRSKIAVYSSDSDVDPVGACVGMKGSRVQSVVQELRGEKIDIVLWD
                     NDPARFVCNAIAPAQVAKVIIIEKRKSMELIVPDDQLSLAIGRKGQNVRLAASLTGWN
                     IDVFSETKVDEMAKFAKGKLVEVLGLSDSLATVLYGHAFRSVEEIAETDIDEFTGIPG
                     MDKETLCMIHRKAVAVVKYGEQPNQPELGGGEEITVVLEGKPAQEEVMQDDEV"
     gene            complement(1496..1978)
                     /gene="rimP"
                     /locus_tag="VJC18_04235"
     CDS             complement(1496..1978)
                     /gene="rimP"
                     /locus_tag="VJC18_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HLD44620.1"
                     /translation="MQNDTREVLRSLVMPLVTQAGYELVDVELAQEGGRKIVRLFVDK
                     PGGISLGECATISHAVEDVLEVEAVISGAYHLEVSSPGLNRPLRTKEHFEAVIGQTVT
                     VSTEEKIDGRRRYRGVLKQVEGDTLVITIDNQDFKVPLNKLSKANLDYHFKTALTPSS
                     "
     gene            2324..3322
                     /locus_tag="VJC18_04240"
     CDS             2324..3322
                     /locus_tag="VJC18_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44621.1"
                     /translation="MRLTQQEFATLRQSIIDGNGATELSKMLCLSETHQQQLIEALFT
                     SAQAQTTSSEPTILFIVSALPFVPQSRQFAYLMRLIPLFGKIKDAHKANLRNLIDEEV
                     ANNLRPLEQISLAHELSNLLKDSNPIQVEIALGGLVKIIPQLHLVNQKEFLHDILDLS
                     QQSALHELTREALTALLLSLESSHCADLALFFADLLKKENWLSRRYFIIDLLKQIVSL
                     LPENDRLMLADQVAPMIYFSDMTLVKKTISFFATTISLLPITQRFYYAQMIASLINDY
                     NMRADVIDAVEKAIPHLANEHEKLLFLELLSQFQPGQSALLETDHAYIPVYYRSGD"
     gene            3391..5118
                     /locus_tag="VJC18_04245"
     CDS             3391..5118
                     /locus_tag="VJC18_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLD44622.1"
                     /translation="MAELTVYQKNKKFFCVPLDSSQLTIGRSSKNDLILTGDQVSRHH
                     AVIEKMNTVFWLRDMSTAGTFLNDHKLVVPTQLQPGNTIAIADWQLVFASDSSVQAAE
                     TERKETQITKLTEELPGDTTQILKFEPDALFYKQLKPCLIIEDAKNGNRTCLVKKNRL
                     VVGSAKNCDILLDDEFVSKYHAEFRLSDLGFHVVDLDSTNGTFVNNFKIKECYLKGGE
                     KIELGNSRIVAVFEEGSSVTLDPFHDNHFCGIYAQSHSMRLLFSKIQKIAATDMTTLI
                     VGETGSGKEMVARALHDLSDRRHKPYLVINCGAITTTLIESELFGHEKGAFTGADQRH
                     LGAFEQANAGTVFLDEIGELPLELQSKLLRVLEYQTLRRVGGSQDIKINVRIIAATHR
                     DLPSLVEHKAFREDLFYRLYVICLKLPPLRERKEDIPLLTKVFIDKCSPDKLLYLDQS
                     SIEKLKNHSWPGNVRELKNTIMRAIAFCQSNIITASDIEFLLPHKGPAIEMAENADPA
                     TALPQTREEVMAPEALRAQEKSRIEGVLKTTNHDKTRAAKLLGMGRSTLFRKMKSLGI
                     ALDDTDKQE"
     gene            5162..>5715
                     /locus_tag="VJC18_04250"
     CDS             5162..>5715
                     /locus_tag="VJC18_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HLD44623.1"
                     /translation="MKAKERLINACLNKTVDRPPVWVMRQAGRYLPEYRSLKAKHSTK
                     HMMTTPSIAAEITTQPVSILGVDAAILYSDILMICDAMGLGLHFITGEGPQFDFCVRD
                     CDAVNRLHTDNINEKLSFVFETIKLCHKKLPKDFPIIGFAGGPFTVAAYMFSGNHQLN
                     FSAIKNFAWTQPKVYEQLLDIITQL"
BASE COUNT         1487 a         1562 c         1186 g         1480 t
ORIGIN      
        1 gttttttctt gttcttccat aatagtatct gaattcgatg attaaataat caagctgaca
       61 aaaaagaata gccggcattt tcttgccgtt agacttcatc gtcttgcatc acttcttctt
      121 gtgcaggctt gccttccaaa accaccgtga tttcttcacc accacccagc tctggctgat
      181 tcggttgttc accatatttc acaacagcaa ccgctttgcg gtgaatcatg caaagtgttt
      241 ctttatccat gcctggaatc cccgtgaatt catcgatgtc ggtctctgca atctcttcga
      301 ctgaccggaa ggcatgccca taaagcactg tcgccagtga atcactgagt cccagcacct
      361 ccacaagttt tcctttggca aattttgcca tttcatcgac cttcgtttcg ctaaagacat
      421 cgatgttcca ccctgtcaag ctggccgcca aacgcacatt ctgccccttg cgcccaatgg
      481 ccaaagataa ttgatcatct ggaacaatga gttccatgga ttttcttttc tcaatgatga
      541 tgaccttggc aacctgggct ggcgcgatgg cattacagac gaatctcgcc ggatcattat
      601 cccacaaaac gatatcgatc ttttccccgc gcagttcttg cacaacagat tgaacacggg
      661 accccttcat gcccacacag gcccccaccg gatccacatc actgtctgaa gaatacactg
      721 cgattttgga acgtacgcca gggtctctcg ccacattttt gatctcgaca atgccctcgg
      781 atatttcggg aacttccatt tcaaaaagcg attttacaaa ttgaggatct cttctagaca
      841 agatcagcat ttgagcccga gcccgcgtgt cgatctcgat aagcagggct tgtatgcgat
      901 cacctgtttt gaacgtttcc ccctcaacca gttcccgacg ataaatcaca gcttctgttt
      961 tgttgagatc aatcaccagg ttcccttttt caaaacgtct caccacgccg gaaaggattt
     1021 gtcccagctt gctctcatac tcattcacga taacgtcttt ttctgcctcg cgtaattttt
     1081 gaatgatgac ctgtttggcc gtctgtgcgg caatgcgccc cagatctttt tggtcaattt
     1141 taacgccaaa actgtctccc accatggctt caccatccag ctttctggcg gtaatcaagt
     1201 ccatttcgag ttgatcatct tccacttctt cggcgacagt tttatactga aacagttcca
     1261 cctcgcccat ttcgggattg tattgtgcct caaggtcacc gtagaatcct aatttttttc
     1321 tggccgctga catcatagcc gcttcaatgg cttcgatcag cgtctctttt gcaatgccct
     1381 tctctttctg aatttgctca agcacgtgat tgagatcgac tatcatcttc ctctcctttt
     1441 gtatcaagac acctctcatt atactcgcgc ctaaccccct cccgcttcgc agtgattaag
     1501 aagagggcgt tagagctgtc ttaaaatgat aatccaagtt tgccttggat agtttgttca
     1561 aagggacctt aaagtcctga ttatcaattg tgatgaccag cgtgtcaccc tcaacttgtt
     1621 ttaacacgcc gcggtaacgt cgccgcccat caattttttc ttcggtagag acagtgactg
     1681 tttgaccaat caccgcctca aaatgttcct ttgtccgcaa agggcgattc agtccgggag
     1741 aagacacctc cagatggtaa gccccgctga tcacggcttc tacttccagc acatcttcaa
     1801 cagcatgtga aatcgtggca cactcgccca ggctgatccc gcctggtttg tctacaaaaa
     1861 gtcttacaat cttacgcccg ccttcctgcg ccaattccac atccaccagc tcatagcctg
     1921 cttgggtcac aagcggcata acaaggctgc gcaggacttc tcgtgtgtcg ttttgcataa
     1981 gcacccccga cagtgtgcgg gtaaaaaaaa gggggccttc cgtgtaagcc cccaaaagat
     2041 atcgccagat tgagcacgga ccttatagga aaaccctttg ggaaatgcaa taaaaaagcc
     2101 actctcctgt aattcattgc aaacagttgc tcttgttgcc aggcaaggct acaaaaccct
     2161 cttttcctat agaaagacat gctcaggcaa ttttttccta gccaaaacag tcattcgctc
     2221 acaaaacaca ataatctctt gctgatatca acgtgttagc cgacaggctc tctggcatta
     2281 ttcttgctta tagtgtctgt aggacacagg gggagtgtaa acaatgagac tcacacaaca
     2341 agagtttgct acactgagac agagcatcat tgatggaaac ggggcaactg aactatccaa
     2401 aatgctgtgt ttgtcggaga ctcatcaaca acagttgatt gaggcgctct tcaccagcgc
     2461 tcaagcacaa accacgtcaa gtgagcccac catcttgttc attgtttcgg ccttgccctt
     2521 tgtccctcag tcgcggcagt ttgcctacct catgcgactt attcccttgt ttggcaaaat
     2581 caaggatgcg cacaaggcca atttgcggaa cctcattgat gaagaggttg cgaacaattt
     2641 aaggccgctc gaacagattt ctctagcaca cgaactgtca aatctgctca aagactcaaa
     2701 ccccattcag gttgaaatcg ccttaggggg gctcgtcaag atcattcccc agctgcacct
     2761 tgtgaatcaa aaagaatttt tgcacgacat tctcgatctc agccaacaga gtgctctgca
     2821 tgagcttacg agggaggcct taacagccct cctgctctcg cttgagtcca gccattgtgc
     2881 tgacctggca ctcttttttg ccgatcttct caaaaaagaa aattggctct cgcgcaggta
     2941 tttcatcatc gatttgctca aacagatagt atcgctcctg cccgaaaatg atcgcctcat
     3001 gctggcagac caggtcgcgc ccatgatcta tttttccgac atgacgttgg tcaaaaaaac
     3061 catatctttt tttgccacga ccatcagtct attgcccatc acgcagaggt tttattacgc
     3121 ccagatgatc gccagcctga tcaatgacta caacatgcgt gctgacgtca ttgatgccgt
     3181 agaaaaagcg attccccatt tggccaacga acacgaaaaa ttattatttc ttgaactatt
     3241 atcccaattc caacccggac aaagcgccct gctggaaaca gatcacgcgt acattcccgt
     3301 gtattaccgc tccggggact aacctcttcc ccagtatcct tttgacttta ccgtttatcg
     3361 gacataaacc gaaggaaaca ggaggtgctt ttggcagaat tgaccgtcta ccaaaaaaac
     3421 aaaaaatttt tttgtgtccc cttggattca agccagctca cgattggaag gtcctcaaaa
     3481 aacgatctca tcctcacagg tgaccaggtt tcgcgtcatc acgctgtgat tgaaaaaatg
     3541 aatactgtct tctggcttcg cgacatgtct acagcaggaa ctttcctcaa cgatcataag
     3601 cttgtcgtcc ccacacagtt gcaacctggc aacacaattg ccatcgcaga ttggcaactt
     3661 gtttttgcat cagactcatc agtacaggcc gccgaaacgg aacgcaaaga aacacaaatc
     3721 accaagctca cagaagagct ccccggcgac acaacacaaa ttcttaaatt tgagcccgac
     3781 gccttgtttt acaagcaact caagccctgt ctgatcattg aagatgccaa aaacggcaat
     3841 cgcacctgtc tggtcaaaaa aaaccgtttg gtggttggct ccgcaaaaaa ctgtgacatt
     3901 cttttggatg acgagttcgt gtcgaaatac catgcggaat ttcgtctttc cgatctgggg
     3961 tttcacgtgg tcgaccttga ttcaaccaac ggaacgttcg tcaacaactt caaaatcaaa
     4021 gagtgctatc tcaagggcgg cgagaaaatt gagttgggca attcccgcat cgtcgctgtg
     4081 ttcgaagagg gttcctcggt gaccttggat ccttttcacg ataatcattt ttgtggcatt
     4141 tatgcacagt cacattccat gcggttgctg ttttccaaaa ttcaaaaaat tgccgctacc
     4201 gacatgacca ccctgatcgt tggagaaaca gggtctggca aggaaatggt cgcccgtgcc
     4261 ctacacgatc tttctgaccg tcgtcacaaa ccctacttgg tcatcaactg cggagccatc
     4321 acaaccaccc tcatcgaaag cgagttgttt ggtcacgaaa aaggggcctt taccggtgcc
     4381 gatcaaagac atctgggtgc ctttgaacaa gccaatgccg gcactgtgtt tttggatgaa
     4441 attggagagt tgccgttgga actccaatca aaattattgc gcgtcctgga atatcaaaca
     4501 ctcagacgcg tcggtggcag tcaggacatt aaaatcaacg tccgcatcat tgcggccacg
     4561 catcgtgatt tgccttccct ggtcgaacac aaagcctttc gtgaagacct tttttacagg
     4621 ctctacgtca tttgtctcaa gcttcctccc ctgcgagaaa gaaaagagga catcccgttg
     4681 ctcacaaagg tctttatcga caaatgctct cccgacaaac ttttgtacct ggatcagtcc
     4741 tccatcgaaa aactaaaaaa tcattcctgg cctggcaatg ttcgtgaact caaaaacacc
     4801 atcatgcgcg ccattgcttt ttgccaaagc aacatcatca cagccagtga cattgagttc
     4861 ttgctgcctc acaaggggcc tgctatcgag atggcagaga acgccgatcc ggcgactgcg
     4921 ctgccgcaaa ctcgggaaga ggtcatggcc cctgaggcac ttcgggcgca ggaaaaaagt
     4981 cggatcgaag gggttctaaa aaccacaaac cacgataaaa cacgtgccgc aaaactattg
     5041 ggaatgggaa ggtccaccct gtttcgaaaa atgaaaagtc tgggcattgc gcttgacgac
     5101 accgacaaac aggaatgagt ccggacacca ccctttcaac ggttttgccc aagaggaaga
     5161 tatgaaagcc aaggaacgcc tgattaacgc ctgcctcaac aaaaccgtag accgcccgcc
     5221 cgtttgggtc atgcgccaag ccggacgcta tttgccggaa taccgttctc tcaaggcaaa
     5281 acacagcacc aagcacatga tgacgacacc cagcattgct gccgaaatca cgacacaacc
     5341 cgtttctatt ctgggcgtgg atgccgccat tctttacagt gatatcctca tgatctgtga
     5401 cgccatgggt ctggggttgc acttcatcac gggagaaggg cctcaatttg atttttgtgt
     5461 gcgagactgt gacgccgtca atagactgca caccgacaac atcaacgaga agctgtcttt
     5521 tgtgttcgaa acgatcaaac tctgccacaa gaaactcccc aaagacttcc ccatcatcgg
     5581 ctttgcgggg gggcctttca ctgtggcagc ctacatgttt tcgggaaatc atcagctcaa
     5641 tttttcggcc atcaaaaact ttgcctggac gcagccgaag gtttacgaac aattgctcga
     5701 catcatcacg caact
//
LOCUS       DATDUN010000311         3875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_17860, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000311 DATDUN010000000
VERSION     DATDUN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17860"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VJC18_04255"
     CDS             <1..255
                     /locus_tag="VJC18_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLD44624.1"
                     /translation="NIRAGLEPMDTNIRAGLEPAPTGTPLSEIVRQLKTFSARRINKM
                     RGSSSPVWQRNFYEHIIRNENDLNHIREYIIHNPQQRDSV"
     gene            344..1375
                     /locus_tag="VJC18_04260"
     CDS             344..1375
                     /locus_tag="VJC18_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HLD44625.1"
                     /translation="MKKRYGYKWLATLSVCGFFMFCTACGGGGTSTAVDDDNDGTDPT
                     DLDYRALMRTFVQDLSAYAKGLDSNFIIIPQNGHQLLTTDGEVGGAVDEDYVSAIDGV
                     GREDLFYGYDNDNQPTSAVDRDDMLSFMDLAEENEVEVLVIDYCSTESYMDDSYEENH
                     YRGYVSFAADHRDLDNIPDYPATPYGENAEDITTLAEARNFLYLINPDQYATKDDFIA
                     GVVATNYDVLITDFFFQGEEEFTADDVTALKAKANGGERLVISYMSIGEAEDYRYYWQ
                     GDWTVGSPDWLDAENPDWEGNYKVRYWDEDWQDIIFGNNESYLHKIVSAGFDGVYLDI
                     IDAFEYFEE"
     gene            1513..3525
                     /locus_tag="VJC18_04265"
     CDS             1513..3525
                     /locus_tag="VJC18_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HLD44626.1"
                     /translation="MKTIFTKLFRNVILVLMMFFVCCLFVACQSGGTSETTGYWYLYA
                     IDDEGNYSQPTFLQIAQNDSTLTIEDSCDPGADPFSTGSIEGKWVSFTMDFGGEEDGN
                     TAVFMGELSDDGYEIEGTVVQNDLERTAVLLRTLEHVCNPDDPTQMIAEYPAEVTASL
                     SLTDTTPTTDCSDYISNSHYPTPGSTPSAGAKGDSTPSNVDGDDFPLVCVDQNYTSST
                     NKSLDNSVLINAETNLFPGAVFQGSGLLENPVEFDPITYKRSSNNTITLSNVTLPAGC
                     SYSTTITSEYTQAAVNDAIGSLLTCKGGPTGTAAYFTYNAQEVYSSDHMAFQLGLDGK
                     YSEYSASNQFSIDTTTNKNHVVLSFWQVFYTVSFPTPANPSDVFADNSTSCSDGSQMG
                     AGNPPIYVKSADYGRVVYFLMESEFSQQDIKDTLEGAYNGLASSLKVTSGVTRQEVAG
                     KSQVHYAVYGGSAALAVQPIAADEDSMFDAVKSYIASEKAATYSASNPGKPMRYVTEF
                     LASNRPTILNFNVTYDIHSCAPQAASNSFKITASSINSHLYMHEGASTSNMSSVVDTG
                     GSSTSYTFSDDDCAADPENPDCERYVSVELTNGDCFSSGVKLNMYRNGSHIWERDYSH
                     GWSTCGEQYYARWKLNYLTGDYTEDYSKGYHGAHWDSPDWGWVKGE"
     gene            complement(3536..>3875)
                     /locus_tag="VJC18_04270"
     CDS             complement(3536..>3875)
                     /locus_tag="VJC18_04270"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HLD44627.1"
                     /translation="SVLRYFDAAEGLNRLLAPVFTHIWGLPAETVPAILIGILKKEIA
                     VAMLAPLGLTTKQLIIACTILALYFPCVATFFVLLKELGVKDLLKTLSLMIFISLLVG
                     GSMNWLFPNF"
BASE COUNT          993 a          921 c         1007 g          954 t
ORIGIN      
        1 aacatacggg cgggtttgga accgatggac accaacatac gggcgggttt ggaacccgcc
       61 cctacgggga caccattatc cgaaattgtg cgacaattaa aaacattttc ggcacgacga
      121 ataaacaaaa tgcgtggatc atcgtccccc gtttggcaac gcaatttcta cgaacacatc
      181 atccgtaacg aaaatgatct gaatcacatt cgggaataca tcatccataa ccctcaacaa
      241 agggattctg tttgagggat aatggggcgg ggttttccca tataaatgtt gtcaaataaa
      301 acgacattgg acattttctc aaggttgtaa catatgatcc cgtatgaaaa agcgatatgg
      361 atacaaatgg ttggcaactt tgtctgtgtg cggtttcttt atgttttgta cggcctgtgg
      421 gggaggtggg acgagcacgg cggttgatga tgataatgat ggcacagacc cgacagacct
      481 cgattaccgg gcgcttatgc gcacgttcgt gcaagacctt agcgcgtatg ccaaaggttt
      541 ggattcaaat ttcatcatca tcccgcaaaa tggtcatcaa ctgctcacaa ccgatggaga
      601 ggtcggcggc gcagtggatg aggattatgt ttctgccata gatggtgtgg gacgagaaga
      661 tttgttttat gggtacgaca acgataatca acccaccagt gccgttgacc gcgatgacat
      721 gctgtctttc atggatttgg cagaggaaaa tgaggtagag gtgctggtca tcgattattg
      781 ttccaccgag tcctatatgg acgattcgta tgaagagaat cactatcgcg gttatgtgtc
      841 ttttgcggcg gatcatcgtg acctcgacaa cattcccgac tacccggcaa ccccctacgg
      901 cgagaatgcg gaggacatca ccacattggc cgaagcacgg aacttcttgt atcttatcaa
      961 ccccgatcaa tatgccacca aggacgattt catcgctgga gtcgtggcca ctaattacga
     1021 cgtactcatc accgattttt tctttcaggg tgaagaagaa ttcacggccg atgatgtgac
     1081 cgccctcaag gccaaggcta acgggggaga gcggctggtg atttcgtaca tgagtattgg
     1141 tgaggctgag gattatcgtt attactggca gggtgattgg actgtgggca gtcctgattg
     1201 gcttgatgcc gaaaaccctg actgggaggg taattataaa gtgcggtatt gggacgagga
     1261 ttggcaggac attatttttg gcaacaacga atcgtatttg cataaaattg tctctgccgg
     1321 ttttgacggg gtgtatctcg acatcatcga cgcgtttgaa tactttgaag agtgatgtgg
     1381 ggtgctgaat ttttgaagcg gtctgtgctg taggggccgg gcttgaccgg cccttataat
     1441 gggagcgtca aaaaatagcg accctgagca acctgcttgc cgacaggcag gctttattga
     1501 acaggagaaa tgatgaaaac gatttttacg aaactgtttc gaaatgtgat cttggttttg
     1561 atgatgtttt ttgtttgttg tttgtttgtg gcctgccagt caggtggcac gagcgaaacc
     1621 acgggttact ggtatttata tgccattgac gatgagggca attactcgca acccacgttt
     1681 ttgcaaatcg ctcaaaatga ttccaccctg accatcgagg attcctgtga tcccggtgcc
     1741 gatccttttt ccacggggag catcgagggt aagtgggtgt ctttcaccat ggactttggt
     1801 ggcgaagaag acgggaacac agcggtgttt atgggagagc tgagtgatga tgggtatgag
     1861 attgaaggca cggtcgtgca gaatgatctg gagcgaacgg cggtgctctt gaggacgctg
     1921 gaacatgttt gtaaccccga cgatcccaca cagatgatcg cggagtatcc tgcagaagtc
     1981 acggcctctt tgagtttgac cgacaccaca cccaccacag attgttctga ttacatcagc
     2041 aacagtcatt atccaacacc aggctcaacc ccttcggcgg gagccaaagg tgattcaacc
     2101 ccttccaacg tagacggtga tgattttccg ttagtttgtg ttgaccaaaa ctataccagt
     2161 tcaaccaaca agtctttgga caattctgtt ttgatcaacg ccgagaccaa cctgtttccg
     2221 ggtgccgtct ttcaaggttc gggactgttg gagaaccctg tggaattcga ccccatcact
     2281 tacaaacgca gttccaataa cacgatcaca ctcagcaatg tgaccctgcc tgcggggtgc
     2341 agttattcta caaccatcac gagcgaatac acccaggcgg ctgtcaatga tgccataggc
     2401 agtttgttga cttgcaaagg aggtcccaca ggcacggccg cctactttac ctataacgct
     2461 caagaggttt attcgagtga ccatatggcc tttcagttgg gactggacgg aaaatattcc
     2521 gaatattctg cgagcaatca attttcgatc gacaccacca caaataaaaa ccatgtcgtg
     2581 ttgtcgttct ggcaggtgtt ttacacggtc tcctttccca caccagccaa tccctctgat
     2641 gtgtttgccg acaacagcac cagttgttct gatggctcac agatgggggc gggcaatccc
     2701 cccatttatg tcaaaagtgc tgactatggg cgtgtggtct actttctgat ggaatccgaa
     2761 ttctcacaac aagacatcaa agatacgctg gagggcgcct acaacggcct ggcttcttca
     2821 ctcaaggtga cctcaggtgt gacgcggcaa gaagtggccg gcaaatcgca ggtgcattat
     2881 gctgtttacg gggggtcagc ggctctggca gtgcaaccca tcgccgcaga cgaagacagc
     2941 atgtttgatg ccgtcaagag ttacattgcc tcagaaaagg cagcaaccta ttctgcctcc
     3001 aaccccggca aacccatgcg ttatgttaca gaatttttgg caagcaacag accaacgata
     3061 cttaacttca acgtaacgta cgacatacat agctgtgctc cccaggccgc gagcaacagc
     3121 ttcaagatca cggccagctc catcaacagc catctttata tgcacgaggg tgcgtcaacg
     3181 tccaacatga gttctgttgt ggacaccggt ggttcttcca cctcgtacac gttttcggat
     3241 gatgattgcg ccgctgatcc cgaaaacccc gactgcgaac gctacgtgtc tgtcgaactc
     3301 accaatggtg attgtttttc gtccggtgta aaactcaaca tgtaccgcaa tggctcccat
     3361 atctgggaac gtgattacag ccatggttgg agcacctgcg gtgagcaata ctatgcccgc
     3421 tggaagctca attatctgac tggcgactat accgaagact attcaaaagg ctaccacggc
     3481 gcacactggg acagcccgga ttggggctgg gtgaaagggg aatgattagt ttcattcaaa
     3541 aatttggaaa gagccagttc atgctccccc ccacgagcag tgaaatgaag atcatgagac
     3601 tcagggtttt taagaggtct ttgacgccca gttccttgag tagaacaaaa aacgtggcca
     3661 cgcacggaaa atacagggcc aaaatggtgc atgcgatgat gagttgtttg gtcgtgagcc
     3721 caaggggggc cagcatggcc acggcaatct cttttttgag tatgccgatc aaaatggcgg
     3781 gcactgtttc ggcgggcagg ccccagatat gcgtgaacac tggggcaagc aggcgattca
     3841 atccctcagc ggcatcaaaa taacgcagaa cactc
//
LOCUS       DATDUN010000312         1122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_513935, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000312 DATDUN010000000
VERSION     DATDUN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513935"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..479
                     /locus_tag="VJC18_04275"
     CDS             117..479
                     /locus_tag="VJC18_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD44628.1"
                     /translation="MTKKILIVDDASYMRALIKDVLVTHGYEVVGEAEDRAGAVIKHK
                     ELKPDLITMDIVMKNSSGIDAIREITTEDKNAKILVLSAMGHQAMFVEAIQAGASGFV
                     LKPFKPEVLLEEVKRILG"
     gene            539..>1122
                     /locus_tag="VJC18_04280"
     CDS             539..>1122
                     /locus_tag="VJC18_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD44629.1"
                     /translation="MSKKIRVIIADDSALMRKKLGEILNSDHEIEVVAIVRNGRELLD
                     VAFHLKPDVITLDVEMPVLNGLEALGYIMSEMPTPCVMISAFTKLGAKETIKALEYGA
                     VDFVAKTSGTISPDIDFLGPEIINKVKLASRVAVQKLKLIWTEKTKDDAKVLKKPQAM
                     TKVFALASSTGGIQALATILPALPADLPAGVLVV"
BASE COUNT          309 a          237 c          308 g          268 t
ORIGIN      
        1 tttgtgctga agcctttcaa accggaggtg ttgttggaag aagtcaaacg cattttggga
       61 taatgcgttt cgtgaagcgt gaagcgtatc tcgaaatacg agatacggag gcaataatga
      121 ctaaaaaaat tctcattgtc gatgacgcca gctacatgag ggcactcatc aaagacgtgc
      181 ttgtgaccca tgggtacgaa gtggtgggcg aggccgaaga cagagcgggt gctgtgatca
      241 aacacaaaga gctcaaacct gatctcatca ccatggacat tgtcatgaag aactcgtcgg
      301 ggattgatgc catacgggag atcacgacag aagataaaaa cgccaaaatc ctggttctta
      361 gcgccatggg acatcaggcc atgtttgtag aagccatcca ggctggtgcc agcggatttg
      421 tgctgaagcc tttcaaaccg gaggtgttgt tggaagaagt caaacgcatt ttgggataat
      481 gcgtttcgtg aagcgtgaag cgtatctcga aatacgagat acgaaatacg gaggcgtggt
      541 gtctaaaaag attcgagtta ttatcgctga cgacagcgcc ctcatgcgca agaagttggg
      601 tgagatcctc aacagtgacc atgagattga ggttgtggcc attgttcgca acggacggga
      661 actgttggat gtggcatttc atctcaagcc ggatgtcatc acactcgacg tggaaatgcc
      721 tgtcttgaat ggacttgagg cactcggtta catcatgagc gaaatgccaa caccgtgtgt
      781 gatgatcagt gcttttacaa agctgggagc caaagaaacc attaaggccc tggagtatgg
      841 cgccgttgat tttgttgcca aaacgagtgg caccatttct cccgatatcg atttcctggg
      901 tcccgagatt atcaacaagg taaaattggc ttcacgggtc gcggtacaaa agctcaaatt
      961 gatatggaca gaaaagacca aagatgatgc aaaggttctc aaaaaaccgc aggccatgac
     1021 aaaggtcttt gccttggcat cgtctacggg tggtattcag gctctggcaa ctattttgcc
     1081 agccctgcca gccgatttgc cggctggtgt tctggtggta ca
//
LOCUS       DATDUN010000313         6674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_430284, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000313 DATDUN010000000
VERSION     DATDUN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430284"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VJC18_04285"
     CDS             <1..192
                     /locus_tag="VJC18_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLD44630.1"
                     /translation="LLQEKYHVTSVVVSHDMASIFRVADRVALLHDHKMVFTGSIEEF
                     RSGKNVMAKQFVNGELGNE"
     gene            350..1264
                     /locus_tag="VJC18_04290"
     CDS             350..1264
                     /locus_tag="VJC18_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLD44631.1"
                     /translation="MKKQKENIQVQVGAFVAIGILLFMMAIFFLGSKMNILQRYYELI
                     CYFDKVNGLRVGAPVQLAGINVGFIDSISFEDRTEEVDVMVSRVDQQLKKLPPLMKKK
                     QVTVKVKAVMKIDRAYQERIRTDTVASIQTQGLLGDRMVFLTVGSQDARPLKSGEVVS
                     QVIDPSGFNQLVYEGSELIQRAKSFLDNSTGFISNTDSFVTRLNTIMDQVIEGDGFAH
                     EVFYNKEHQQAFSKLDEILGNFEKASVNFSQVSDKINTGKGTLGSLVNDDSLYQDIKM
                     LLGKANRNKLIRSVIRYTLETKEKEQLK"
     gene            1403..3484
                     /locus_tag="VJC18_04295"
     CDS             1403..3484
                     /locus_tag="VJC18_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HLD44632.1"
                     /translation="MTLPNELINVINTSDEIRHFRELNWKGAFGDYLALFQETPAVAR
                     IAFQRIYDMILSYGTDEYVEYKKHITRFTFFSDPFEDGKDAVFGIDIHLMKLVNVFKA
                     AALRYGPEKRVILLHGPVGSSKSTIVRLLKKGLENYSRIPEGALYSFRWKKLRQNDAE
                     LDRIFGLVDTIDDPIHGEPLYLIPKKAREQFLDEVNKKLSEKDRIVLEGDLSPISRFI
                     YTELFNHYEGDWAKVVEHVEVYRVSLSEKDRIGIGTFQPKDEKNQDSTELTGDINYRK
                     IAEYGSESDPRAFNFDGEFNIANRGVIEFVEMLKLDVAFLYDLLGASQEHKIKPKKFA
                     QTDIDEVIIGHTNEPEYRKLQNNEYMEALRDRTIKIDIPYITRVSQEIKIYEKDFSNR
                     KIKGKHIAPHTLEVAAMWAILTRLEEPKKANLTLMQKLRLYDGKSLPGFTEDNVKELR
                     KEAGREGLEGISPRYIQDKMSNVLVSSNTTSVNPFMLINELESGLRHHSLLNAEETRK
                     RYQEMFTVVKEEYEDVVKNEVQRAITADEDAIQRLCANYIDNVKAYTQKEKVRNKFTG
                     QNEEPDERLMRSIEEKIDIPESRKEDFRREIMNYIGALAIENKKFDYRTNERLHKALE
                     LKLFEDQKDSIKLSSLVTSTMDEDTQEKIEVVKSRLIKNYGYNDESATDVLNFVASIF
                     ARGDIKNRDNR"
     gene            3587..4693
                     /locus_tag="VJC18_04300"
     CDS             3587..4693
                     /locus_tag="VJC18_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HLD44633.1"
                     /translation="MAEKIEQDLSRFKKIIRGKVKKELGKFVSSGELIGRQGKKLISI
                     PIPRIDIPRFKFTDKQQGGVGQGEGQAGDPLDQGDEDGYGKAGDSPGQHLLEVDISIE
                     ELVDMLGEELALPNIQPKNNEKFEEIKNVYRGIRRSGPESLRHVKRTYKQALKRQIFT
                     GEYNPLKPRVVPYKEDRRYRTYKQERLPNFKAVIIYMMDVSGSMGNEQKEIVRLESFW
                     IDAWLKKQYKGIETRYIIHDAVAREVDQETFYHTRESGGTIISSAYKLCQEIIEADYD
                     PMAWNIYPFHFSDGDNWSANDTQECVKLVDEYFSKVANVFCYGQVESEFGSGQFLKDL
                     REKFAGKNDNVITSQIENRDGILNSIKDFLGKGK"
     gene            4690..6204
                     /locus_tag="VJC18_04305"
     CDS             4690..6204
                     /locus_tag="VJC18_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HLD44634.1"
                     /translation="MIQNTNLSTELEEWRAKLEAWAKEYGLDFYETIFEVLDWNQINM
                     VAAYSGFPNRYPHWRFGMEYEQLAKSYSYGLSKIYEMVINNDPCYAYLLHSNNLVDQK
                     MVMAHVFAHCDFFKNNIFFGPTPRNMMDQMANHKTRVQRYIDNIGYDKVESFIDTCLS
                     IENLIDYQRPFFKQPAVEPKQDDDDRSVQEERGVHKLKVEKDYLETYINPKAFIDLQK
                     QRIEEDRKKEERFPLHPERDVMGFLLEHAPLSRWEYDVLHIVRNEAYYFAPQAQTKIM
                     NEGWASYWHSKIMTEKALLDSELIDFADHHSGTMAMSAQSLNPYKIGIELIRNIEDRW
                     NKGQFGKEYDECDDMVVRKNWDKQLGLGRQKIFEVRKIYNDIMFLDEFLTPEFCREYK
                     LFAFTFNPLSNRYEISDRDFAVVKQRLLAALTNFGQPIIAIVDANHDNRGELKLRHEH
                     EGVDLDIQYARETLQNIFKIWKRPVLIETKFNNGKKIFQYDGNEHKILESKEGI"
     gene            6498..>6674
                     /locus_tag="VJC18_04310"
     CDS             6498..>6674
                     /locus_tag="VJC18_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLD44635.1"
                     /translation="MNKSDLIEYLSEKMAQPKKKTEDVVNHIFDTMTNVLAEGGRIEI
                     RGFGSFVTKDYGCYT"
BASE COUNT         1903 a         1411 c         1746 g         1614 t
ORIGIN      
        1 ttgttgcagg aaaaatatca cgtgacctcg gtggtggtgt cgcacgacat ggccagcatc
       61 ttccgggtgg cggatcgtgt ggcgcttttg catgatcaca aaatggtgtt tacgggaagt
      121 attgaggagt ttaggagcgg aaagaatgtg atggctaaac agtttgtgaa tggggagttg
      181 gggaatgaat aacagctgaa ccgcaaagat ttgcggttca gaatatcgaa taaagaacaa
      241 ggaatcatga atttcgaaat aggtcatccc tgcgaaggca gggatccaga ataaccagaa
      301 aataacacac atggattcca gcctacgctg gaatgacaaa ccgtgctata tgaaaaaaca
      361 aaaagaaaat attcaggtgc aggtgggggc ctttgtggcg atcgggattt tattattcat
      421 gatggccata ttctttctgg gttctaaaat gaatatcctg cagaggtatt acgaactgat
      481 ctgctatttc gacaaggtca atggactgcg agtcggtgcc cctgttcagt tagcgggtat
      541 caatgttggt tttatcgatt ccatttcctt tgaggaccgg accgaagaag tggatgtgat
      601 ggtgtcaagg gtggatcaac aactcaagaa actgccaccc ctgatgaaga aaaaacaggt
      661 gacggtcaaa gtgaaggcgg tcatgaagat cgatcgcgct tatcaggaac gcatccgcac
      721 ggacactgtg gccagtattc aaacacaagg gcttttgggg gatcgtatgg tgttcctgac
      781 cgtagggagt caggacgcga ggcctctcaa aagtggcgag gtggtttcac aggtgataga
      841 tccttcgggg ttcaatcagt tggtctacga agggagtgaa ttgattcaga gggcgaaaag
      901 ttttctcgac aattccaccg gattcatcag caacacagat tcatttgtga ccagactcaa
      961 cacgatcatg gatcaggtga ttgagggaga tggattcgca cacgaggttt tttacaacaa
     1021 ggaacaccaa caggcgtttt ccaaactcga tgaaatcctt ggcaatttcg aaaaggcgag
     1081 cgtgaatttt tcccaggtct cagacaagat caataccggg aaggggacct tggggtcact
     1141 ggtgaatgac gattccttgt atcaggatat taaaatgtta ttgggcaaag ccaatcgaaa
     1201 caaattgatt cgctccgtca tcaggtacac actcgaaaca aaagagaaag agcaattgaa
     1261 ataaaatact tgtacatgtt acaatgaaaa tgtaagccat cacttcccgt tagagagact
     1321 tctttgatcc gcatcggaat tgattttttg caagttttaa ttgccgtgct ggctggcaac
     1381 ggcgcaaatc aaggagatat tcatgacgtt accaaacgaa ttaatcaatg tcatcaacac
     1441 atcagatgag atcaggcatt ttcgggagct gaactggaag ggcgcttttg gtgattatct
     1501 ggccttgttt caggaaaccc ccgctgtggc cagaattgcc tttcagagaa tttacgacat
     1561 gatcttgagt tacggcacag atgagtatgt cgagtacaaa aaacacatca cccggttcac
     1621 gtttttctct gatcctttcg aagacggcaa agacgcggtt tttgggatcg acattcatct
     1681 catgaaactg gtgaacgtgt tcaaggcggc cgctttgcgg tatggtcccg aaaaacgcgt
     1741 gattcttttg catggtcctg tggggtcatc caagtccact attgtgcgtc tgctcaaaaa
     1801 aggcctggag aattattcgc gcatccctga aggggccctg tacagttttc gctggaaaaa
     1861 actcagacag aacgatgctg aactcgatcg catttttggt ctcgtggaca ccattgatga
     1921 tcccattcat ggtgaaccgt tgtacctgat tcccaaaaaa gcgcgtgaac agtttctgga
     1981 cgaggtcaac aaaaagttgt cggagaaaga tcgtatcgtc ctggaagggg atttatcgcc
     2041 gatcagccgg ttcatttaca cagagttgtt caatcattat gaaggtgact gggccaaggt
     2101 ggtggagcat gtcgaggtgt accgtgtgtc tttgtccgaa aaggaccgca tcgggattgg
     2161 gacctttcag cccaaggacg aaaagaatca ggattccact gaactcacgg gggatatcaa
     2221 ttaccgcaag atcgccgaat atggttctga gtccgatccc cgcgcgttca atttcgatgg
     2281 tgagttcaat atcgccaacc gcggcgtgat cgagtttgtc gaaatgctca agctcgacgt
     2341 ggcctttttg tacgacttgt tgggggcctc gcaggaacac aagatcaagc ccaaaaagtt
     2401 tgcccagaca gacatcgacg aagtgattat cggtcacacc aatgagcctg agtaccgtaa
     2461 actgcagaac aacgaataca tggaagccct gcgtgaccgc acgatcaaga tcgacatccc
     2521 gtacatcacg cgcgtgtcgc aggaaatcaa aatctacgaa aaggattttt ccaacagaaa
     2581 gatcaagggc aaacacattg ccccgcacac tctcgaggtg gccgccatgt gggccatcct
     2641 cacgcgcttg gaagaaccca aaaaggccaa cctgacgctc atgcagaaac tccgtttgta
     2701 cgatggcaag agcctgccgg gattcacaga ggataacgtc aaggaattgc gcaaggaggc
     2761 cggacgcgaa ggtttggagg gcatttcccc gcgttatatt caggacaaga tgtccaacgt
     2821 gctcgtgagt tctaacacca cgtcggtcaa cccgttcatg ctcatcaacg agctggaaag
     2881 cgggttgaga catcattccc tgcttaacgc tgaggaaacc cgcaagcgat atcaggaaat
     2941 gttcacggtc gtgaaggaag agtacgaaga cgttgtcaag aacgaggtac agagggcgat
     3001 caccgcggat gaggacgcca ttcagagatt gtgtgccaat tacattgata acgtcaaagc
     3061 gtatacacag aaggaaaagg tgcgcaacaa atttacgggg cagaatgagg agcccgatga
     3121 aaggctcatg cgctctatcg aggaaaaaat cgatatcccc gagtcccgca aggaagattt
     3181 ccgtcgcgag atcatgaact acattggggc tttggcgatt gaaaacaaaa aatttgatta
     3241 ccgcaccaac gagagattgc acaaggctct ggaactcaag ctgtttgagg atcagaagga
     3301 ttcgatcaaa ctgagttctc tggtcacctc cacgatggac gaggacacgc aggagaagat
     3361 tgaggtggtt aagtcgcgtc tcatcaagaa ctatggctac aatgacgaga gtgccaccga
     3421 tgtattgaat ttcgtggcct ccatcttcgc gaggggcgat attaaaaata gggataatcg
     3481 ataaattagt ggatcgtggg tcgttgatcg tagatcgtgg ggtgttaact gtacgattca
     3541 cgattcatac cctctggggc acaagcgatt tacgattcgc gttttcatgg ctgaaaaaat
     3601 tgaacaagat ctctcccgtt ttaaaaagat catccgtggc aaggtcaaaa aggagctggg
     3661 caaatttgtc tcgtccggtg aactcattgg caggcagggg aaaaagctca tcagcatccc
     3721 catcccccgc attgacatcc cgcgtttcaa gtttaccgac aaacagcagg gaggggtagg
     3781 tcagggagag gggcaggcgg gggaccctct ggatcagggt gatgaggatg gttatggcaa
     3841 ggccggtgat tctcccggtc agcacctgtt ggaggtcgat atttcgatcg aagaactggt
     3901 cgatatgttg ggcgaagagt tggccctgcc caatatccaa cccaagaaca atgaaaaatt
     3961 cgaggagatc aaaaacgtct atcgcggtat ccgtcgttcg ggtccggaat cgttgcgtca
     4021 tgtcaagcgg acctacaagc aggcgttgaa gagacagata tttaccggcg agtacaatcc
     4081 tctgaaaccg agggtcgtgc cttacaagga agatcgtcgg taccgcacct acaagcaaga
     4141 aagactcccc aatttcaagg cggtgattat ttacatgatg gacgtttcgg gttccatggg
     4201 caatgaacaa aaagagattg tccgtttgga atccttttgg atcgatgcct ggctcaagaa
     4261 gcaatacaag gggatcgaaa cacgttacat tattcacgat gccgtggccc gcgaggtcga
     4321 tcaagagact ttttaccaca cgcgcgaatc cggtggcacc atcatctcat cggcctacaa
     4381 actgtgtcag gagatcatcg aagcggatta cgatcccatg gcgtggaata tttacccgtt
     4441 ccatttttcc gacggagaca actggtcggc caacgacacg caggaatgtg tcaaacttgt
     4501 cgacgagtat ttttccaagg tggccaacgt cttctgttac ggtcaggtcg agagtgaatt
     4561 cgggagcggt cagtttttga aggacctcag ggaaaagttt gcgggcaaga acgacaacgt
     4621 gatcaccagc cagatcgaaa acagggacgg gattctcaat tcgatcaagg attttctggg
     4681 gaagggcaaa tgattcaaaa cacaaattta tcaaccgaac tggaagagtg gcgggccaaa
     4741 cttgaggctt gggccaagga atatggcctc gatttttacg agaccatctt cgaggtgttg
     4801 gactggaacc agatcaacat ggtggcggcc tatagcggct ttcccaacag gtatcctcat
     4861 tggcggttcg gcatggagta tgaacagctt gccaagagtt attcatacgg gttgtccaaa
     4921 atatatgaga tggtgatcaa caatgaccct tgttatgctt atttgttgca tagcaacaat
     4981 ctggtcgatc aaaagatggt gatggcgcac gtgttcgccc actgtgattt tttcaaaaac
     5041 aacattttct ttggtcccac gccccgcaac atgatggacc agatggctaa tcacaaaaca
     5101 cgtgtacagc gttacatcga caacatcggg tacgacaagg ttgaatcatt catcgatacg
     5161 tgtttgtcga tcgaaaatct tattgattac cagcgacctt ttttcaagca accggcggtt
     5221 gagccaaaac aagatgatga tgaccgttcg gtgcaggaag agcgcggtgt gcacaaactg
     5281 aaggttgaga aggattattt ggagacgtac atcaatccca aggcattcat tgatctccaa
     5341 aaacaacgca tcgaagaaga caggaaaaaa gaggagaggt ttcctttgca cccggagcgg
     5401 gacgtgatgg gttttctttt ggaacatgcc ccgttatccc gttgggaata tgatgtgttg
     5461 cacatcgtgc ggaacgaggc ctactatttc gcaccgcaag cccagaccaa aatcatgaac
     5521 gaaggttggg cgagttattg gcattccaag atcatgacag aaaaggccct gttggattcg
     5581 gaactcatcg atttcgccga tcatcattca gggacgatgg ccatgagtgc ccaatcgctc
     5641 aatccttaca agattggtat tgagttgatc agaaacattg aagaccgttg gaacaaggga
     5701 caatttggca aggaatatga cgaatgcgac gatatggttg tgaggaaaaa ctgggacaaa
     5761 caacttggtc tggggagaca aaaaatattc gaggtaagaa aaatttataa tgatattatg
     5821 tttcttgacg aatttctcac accagaattc tgtcgcgaat acaaactctt tgcctttact
     5881 tttaatccgc tgagtaaccg ctacgaaatt tctgatcgcg atttcgctgt ggttaaacaa
     5941 cgtctcctgg cggcgctcac aaattttggg caacctatca ttgcgattgt ggatgccaat
     6001 catgacaaca ggggtgaact gaaattgcgc catgaacacg agggtgtaga tctggatatc
     6061 cagtacgcca gagaaacgtt gcaaaatatt ttcaagattt ggaaacgtcc cgtactcatc
     6121 gaaaccaagt tcaataatgg caaaaagatt tttcagtatg acggcaatga acacaagatt
     6181 ctcgagagca aagaaggtat ctgaagcctg attatcactg atgttttttg gccacatctt
     6241 ttttatccac aggcgacatc gcccgcaaat acaataatat caaggtttta ccgtttgttg
     6301 acagattgct cagagaatgt taagatttgg gaagtgatat taagcactta gtccttgtgg
     6361 ataagttgtt atcacaggag ttgcagtttg ggagtgtgtt gtttgaatga tggcgactgt
     6421 gtttggtttg tgtttggttt tgtcaatcaa agccgtcttg gctgagtggt cagcggcggc
     6481 gtgccaaggg agtggatatg aataaatcag atctgatcga gtatctgtca gagaaaatgg
     6541 ctcagcccaa gaagaaaacc gaagatgtgg tgaatcacat tttcgatacg atgaccaatg
     6601 tattggcaga gggaggccgc attgagattc gcgggtttgg gagctttgtg accaaggatt
     6661 acggttgtta caca
//
LOCUS       DATDUN010000314         1141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_181051, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000314 DATDUN010000000
VERSION     DATDUN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181051"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VJC18_04315"
     CDS             complement(<1..1082)
                     /locus_tag="VJC18_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44636.1"
                     /translation="MLRKIFNTVLLLSLFVGFMTMISFSAKAETLVSRSTPRMMQGVR
                     PLGMGGAFVAVAGTDENALFYNPAAINDFPQEFHFQFLLPTAEFSYKSLNFLISDVPD
                     LVDDIDAANGDSAKIDVLDAFAASNTGRYEEIAARGNVITMMHKYITASLFYETHGVV
                     ALLNPSSSTVDMELMGQAGLQLGSAYSFFDDYLQAGVAVKLMERYLVDETVTQRDVIA
                     NAEFNDIIDYESFGFGVGVDVGFKGTFPVSGKTWDYLKPKFAFTVQDVGHTRFFAGDP
                     VGRQKESMTFGLAVHPDFWKFKSIFAIDMRDLEYRGDFFTKFHAGYELTWPEISKILR
                     AVSLRVGVNQIYVTGGVGFDFKYFKM"
BASE COUNT          311 a          296 c          264 g          270 t
ORIGIN      
        1 ttcatcttaa agtatttgaa atcgaacccc acaccgcctg tgacgtagat ctggttgacg
       61 cctactctga gagaaaccgc tcgcaaaatc ttgctgatct ctggccaggt aagctcatag
      121 cccgcatgaa acttggtgaa gaaatcgccg cggtattcca aatcgcgcat gtcgatggca
      181 aaaattgatt tgaacttcca gaaatcggga tgcacggcca gaccaaacgt catggactct
      241 ttttgacggc caacggggtc accagcgaaa aaccgtgtgt gccctacgtc ttgcacggta
      301 aaggcaaact tgggttttaa ataatcccag gtcttgccac tcacagggaa cgtccccttg
      361 aaacccacat caacccccac accaaaacca aaggattcgt agtcgatgat gtcgttgaat
      421 tctgcgttgg caatgacgtc tctttgggta acggtttcgt caaccagata ccgctccatg
      481 agctttaccg cgacaccggc ctgtaaataa tcatcgaaaa agctgtacgc cgaccccaat
      541 tgcaatccgg cttgtcccat cagttccata tccaccgtgc tggaagaggg attgagcagg
      601 gccacaacac catgtgtttc gtaaaaaagt gatgccgtga tgtatttgtg catcatggtg
      661 atcacattgc ctcgggctgc gatttcttcg tagcggcccg tgttgctcgc cgcaaaggcg
      721 tctaacacgt cgatcttggc cgagtcgcca ttggcggcgt cgatgtcatc gaccagatcg
      781 ggcacatcgc tgatgagaaa gttaagcgac ttgtacgaaa actccgctgt tggcagtaaa
      841 aactgaaaat gaaattcctg tggaaaatca tttatggctg ccgggttata aaaaagtgcg
      901 ttctcgtcgg taccagcaac ggcaacgaaa gcaccgccca tacctaatgg cctgacccct
      961 tgcatcatgc gaggcgtgga acgcgaaacc aatgtttctg cctttgcaga aaaactgatc
     1021 atggtcatga agcccacaaa caggctcagc aaaagcacag tgttgaatat ttttcttaac
     1081 atgagaaatc cccctttact ggattaaagt gatgtgttca ctaaataaca cgcctcaact
     1141 g
//
LOCUS       DATDUN010000315         1922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_514274, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000315 DATDUN010000000
VERSION     DATDUN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514274"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VJC18_04320"
     CDS             complement(<1..1376)
                     /locus_tag="VJC18_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLD44637.1"
                     /translation="MKEKILVVDDEAAIKKALTKFLTSQEYDVDSASDGAEAVELAKN
                     KIYDLVITDLKMPNMTGIELITELKKINPQTVTLVMTGFGTIDSAVEAIKAGAFHYFT
                     KPFELDDVAMLIDKALKYSRLEDQNQRLRRQVSMRYQFENIVGCSEKLKEVFDVVQKV
                     ADTDSTVLVTGESGTGKELIARAIHFNSKRAQNSLVAVNCAAIPENLLETELFGYVKG
                     AFTGAVNSKIGKFEQAQGGTVFLDEVGDMSMKLQVKLLRVLQERKLEPVGSTQTIDID
                     VRIIAATQHHLEELVEQGLFREDLYYRLNVIDVPVPALRDRVSDIPLLLNHFMTMYAA
                     ANQVKRPVLSEEVINLFMSYKWPGNVRELENTIERLVVLKPGQQIAIDELPDKFSKLT
                     NAQFLKPGFSIPEGGISLKNVVEDFENTLIEKALAKTNWNKNRAASLLKLNRTTLVEK
                     IKKKNLIKP"
     gene            complement(1404..>1922)
                     /locus_tag="VJC18_04325"
     CDS             complement(1404..>1922)
                     /locus_tag="VJC18_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44638.1"
                     /translation="IVRHLLDFSREKSDEKMEPVFLGDVVSKTMSLVSVSAKQVGVQV
                     VQDLDPDLPTITGDFNRLQQVVLNLVTNSVFAMKDKGGTLSLCTFSNPERCKVFLQIA
                     DTGVGIAPKDLDRIFDPYFTTKEPGEGTGLGLSICYKIIEAHKGKIDVESDVGLGTTI
                     TIAFVANPATRG"
BASE COUNT          446 a          530 c          441 g          505 t
ORIGIN      
        1 ggcttgatga gattcttctt cttgattttt tcgaccagtg ttgttcgatt caacttcaac
       61 aaagacgccg ccctgttctt gttccagttg gttttggcca acgccttctc aatgagcgtg
      121 ttttcgaaat cctccactac atttttgaga gaaatccccc cctcaggaat ggaaaagccc
      181 ggcttgagga actgggcatt cgtcagttta ctgaatttat cgggcaactc gtcaatagca
      241 atctgttggc cgggtttgag caccaccagt ctctcgatgg tgttttccaa ctcacggaca
      301 ttgcccggcc atttgtagct catgaagagg ttgatcacct cctcagacag gaccgggcgt
      361 ttgacctgat tggccgccgc gtacatggtc atgaaatggt tcaagagcag ggggatatca
      421 ctcacgcgat ctctcaaggc agggacaggc acatcgatca cattcaggcg gtaatacaaa
      481 tcctctcgga aaagtccctg ttccaccaac tcttccaggt gatgctgtgt ggcggcaatg
      541 atgcgcacgt caatgtcgat ggtttgcgtg gaccccacag gttccagctt gcgctcctgc
      601 aacacccgca aaagcttgac ctgaagcttc atgctcatgt cgccaacttc atcaagaaaa
      661 acagtgcccc cctgagcctg ctcgaacttg cctatttttg aattgacggc gcccgtgaag
      721 gcccctttga cgtatccaaa gagttcggtc tccaaaaggt tttcggggat ggccgcacaa
      781 ttcacagcca ccagggagtt ctgcgcacgc ttgctgttaa agtgaatggc ccgcgcgatc
      841 aactccttgc cagtcccgct ttcgcctgtc accaaaacgg tggaatccgt atcggcaact
      901 ttttgcacca catcaaacac ttccttgagc ttttcggaac accccacgat attttcgaac
      961 tgataccgca tgctgacctg acggcgcaaa cgctggttct ggtcttccag cctcgaatat
     1021 ttgagggcct tgtcgatgag catggccaca tcatcgagtt caaacggctt ggtaaagtaa
     1081 tgaaaggccc cagccttaat ggcctccaca gcactatcga tggtgccaaa accggtcatg
     1141 accaatgtca cggtttgcgg attgattttt ttgagttccg tgatgagttc aatccccgtc
     1201 atgttgggca tcttcaaatc ggtgatgacc aaatcgtaaa tcttgttctt ggccagctcc
     1261 acagcctctg cgccgtccga agcactgtcc acatcgtatt cctgggatgt cagaaatttg
     1321 gtcagcgctt tcttgatggc cgcctcatca tcaactacca gaattttttc tttcataagt
     1381 ttctcctcac ctgtgcgttt gccctatccc ctcgtagcgg gattggccac aaaagcaatc
     1441 gtgatcgtcg ttcccaaccc cacatcactt tcgacatcga ttttcccttt gtgagcctca
     1501 atgatcttgt aacatatgga tagccccaaa ccagtgcctt cgccaggttc ctttgtggtg
     1561 aaatagggat caaaaatcct gtcgagatcc tttggggcaa tgcccacacc ggtatcggca
     1621 atctgcaaaa acactttaca ccgttcgggg ttcgagaagg tacaaagaga gagtgtacca
     1681 cccttgtcct tcatggcgaa cacactgttg gtcaccagat tgagcacgac ctgctgtaaa
     1741 cggttgaagt ctccggtgat cgtgggtaaa tcggggtcaa gatcctgtac cacctgcaca
     1801 cctacctgtt ttgcgctcac cgaaacgaga gacatggttt ttgacacgac gtcacccaaa
     1861 aacacgggct ccattttttc gtccgatttt tcgcgtgaaa aatccagaag gtgtcgcaca
     1921 at
//
LOCUS       DATDUN010000316         3599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_18426, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000316 DATDUN010000000
VERSION     DATDUN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18426"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VJC18_04330"
     CDS             complement(<1..429)
                     /locus_tag="VJC18_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44639.1"
                     /translation="MDKKTDRRSTIHDPRSFMPTPQQAMEYIWNKIPKQDDGTIRYQP
                     GDLDYLYNNGFVNSRKYSREQWKSAFRAYARPDGTYRIKKNQFIALCVFREVSPLKIF
                     DASKIKDGPWTDEELRTLYHEAIVPSSELRPESFTVFVDTL"
     gene            complement(465..1196)
                     /locus_tag="VJC18_04335"
     CDS             complement(465..1196)
                     /locus_tag="VJC18_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44640.1"
                     /translation="MATPYETMLYIWDKIPKNPDNHTITYSDADLIYLYHEGFVNTRK
                     FSMKDWKKAFVPYLGKDQKYTLNRNQFLAIRKYRYNGPSDEMFDCSRIREGAWTDAQL
                     TVFYRRSVRQASEMPEEMFWKLINDLKKNGYSDENGNMIFDADIKKGLSYVVAQFPSP
                     RTRLEYKVKTLREKGEEGLKALEQKKTKSQFSVGTTAVETTLASLQKLQSSAPKASQV
                     DTTQTATQEEVDIKKLRKPKGKLFG"
     gene            complement(1284..2615)
                     /locus_tag="VJC18_04340"
     CDS             complement(1284..2615)
                     /locus_tag="VJC18_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44641.1"
                     /translation="MKIYYPIIYEGKRPNENPLYLLSYLRSHSDIAFSTHTLTPQNMS
                     ELENSEDNYILLSVTSLSPFYKFHHLLMMLRKTFRHIKCKIIVGGAVFYILNHQDVLK
                     HYPEITHICVGKGEEVLKAIIEKNLPRGVYSAQNFDRVQKYLLIPEFAVKDVVMLTFR
                     DSRCSWGKCLFCHHQTRCMYPNKNAADVAHEVMHYIDAFGTRYFYFFDNHMEPETLAE
                     FLEILFHRGYAKFHPQFYVFGIRVDGNLKALNPILQKWPKATIDGIAWGVEFYDQEIL
                     DRFKKGTSLAQIDTALDFFPKYGTVNDVYILLGLPTITQKNIAHHERFIEKQYNNIFR
                     LMTSFFLFNDGLKISEHSDEFKINKLDYQFTLQDFFMYAVDIPPIRTVYTDFTSWDED
                     LQKYASRGETFLKYLSLYQKFNKIGTCPRLSFLDEKTHTILKKIRPDLYFG"
     gene            complement(2612..3157)
                     /locus_tag="VJC18_04345"
     CDS             complement(2612..3157)
                     /locus_tag="VJC18_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44642.1"
                     /translation="MSSSKEVMEQIWATMKKADGKNVVFKQADVDYLYYSGFVDEKRC
                     TIEDWQKAFMPFSQDGKTFVMNQQQFASLAPFRYEGVVKEIFDPIKLRDGIWTKEQLR
                     MLFERSIKPCSAISEEVFWKFIEGCQPKNDASEQDGFFLDKGVKVGLREFMEQFPSNR
                     RRLEKTVRQVACKKIQRGQKT"
     gene            complement(3255..>3599)
                     /locus_tag="VJC18_04350"
     CDS             complement(3255..>3599)
                     /locus_tag="VJC18_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44643.1"
                     /translation="EADPSLIEQTTQQRTESTASATGTVAQEGSYYDESRGMKEAGES
                     RSFNNFESNNILNIDDQSVASTELQPPANKKLKSFEAGVTKNDLNPVGLKSASSEAQA
                     SVTASGSQLTSV"
BASE COUNT          880 a          776 c          884 g         1059 t
ORIGIN      
        1 gagtgtgtcg acaaacacgg taaaactctc cggtctcagc tccgaactgg gaacgatagc
       61 ctcgtggtag agcgttctga gttcctcatc cgtccagggg ccgtccttga tttttgaggc
      121 atcaaatatt ttgagcggtg acacctcacg gaaaacgcac aaggcgatga actgattttt
      181 tttaatgcgg tacgtgccat cgggacgagc gtaggcgcga aacgcgctct tccattgttc
      241 ccgagagtat tttcgagaat tcacaaaacc gttattgtag agataatcga gatccccagg
      301 ctggtagcga atggtgccgt catcctgttt tgggatcttg ttccagatgt actccatggc
      361 ttgttgcggt gtgggcataa aggaacgtgg atcgtgaatc gtggatcgtc gatcggtttt
      421 tttatccacg atccacaatc cacaatccac gatcaacgct gtgactatcc aaataacttt
      481 cccttgggtt tgcgcagttt tttaatgtcg acttcctcct gggttgcagt ctgtgtcgtg
      541 tcaacctgtg atgccttggg agcagaggat tgcagttttt gcagggaagc caaagtggtt
      601 tcgacagcgg tggttcccac tgagaattga ctctttgttt tcttttgttc cagtgccttt
      661 aagccttctt ctcctttttc tctcagagtt ttgaccttat actcaagccg ggtcctgggc
      721 gaggggaatt gggcaaccac gtaagaaagg ccctttttaa tatcggcatc gaaaatcata
      781 ttgccgtttt catcggaata gccgtttttt ttgagatcgt tgatcaactt ccagaacatt
      841 tcctcgggca tctctgatgc ttggcgtaca gagcgtctat aaaagacagt cagttgggca
      901 tctgtccacg ccccttcgcg aatacgactg caatcaaaca tctcgtcgct ggggccgttg
      961 tagcggtatt ttctgatcgc aagaaactgg ttgcggttga gggtgtactt ctgatccttt
     1021 cccaggtagg gaacaaaggc cttcttccaa tccttcattg aaaacttgcg ggtgtttaca
     1081 aaaccttcgt gataaagata aatcaagtcg gcatccgaat aggtgatggt gtggttatcc
     1141 gggtttttgg ggattttgtc ccaaatgtac aacatggttt cgtaaggtgt ggccataaag
     1201 gtcctccgtt ttctgggaac attgtagcac gcgcaacccc atcagatcaa agagaaactc
     1261 accaagtcat tattcagggt ctgctaccca aaatacaagt cgggtctgat ttttttaaga
     1321 attgtgtgtg ttttttcgtc caggaaactc agacgcgggc aggttcctat tttgttgaac
     1381 ttctggtaca gcgacaggta tttcaaaaaa gtttcgccgc gtgaggcgta tttttgcagg
     1441 tcctcatccc acgaggtaaa atcagtgtag actgtcctga tcggagggat atcaactgcg
     1501 tacatgaaaa aatcctgcaa agtgaattga taatcgagtt tgttgatttt gaattcgtca
     1561 ctgtgttcac tgatcttcag accgtcgtta aataaaaaaa acgaggtcat gaggcgaaaa
     1621 atgttgttat actgtttttc aatgaagcgt tcatgatgag caatgttttt ttgcgtgatt
     1681 gtgggtagtc ccaacaatat atagacatcg ttaacggtgc catattttgg aaaaaaatcg
     1741 agggccgtat cgatctgtgc cagcgaggtt ccttttttaa aacggtccag aatttcttga
     1801 tcataaaact caacacccca ggcgatgccg tcgattgttg cctttggcca cttttgcaga
     1861 atgggattca atgccttgag gtttccatcc acgcgaatcc caaaaacgta aaactgcgga
     1921 tgaaactttg cataaccacg gtggaacagg atctccaaaa actcagccag cgtttcgggc
     1981 tccatgtgat tgtcgaaaaa ataaaagtag cgagtgccaa aagcatcgat gtaatgcatg
     2041 acctcatgtg ccacatcggc cgcgttttta ttgggataca tgcatcgagt ttgatggtga
     2101 cagaacaggc acttgcccca ggagcatcgg ctgtccctga aggtgagcat cactacgtct
     2161 ttcacggcga attctggtat cagcaggtat ttctggactc tgtcgaaatt ttgcgcgctg
     2221 tagacgccac ggggcaggtt tttttcgatg atggccttga ggacctcttc gcccttgccc
     2281 acgcagatgt gggtgatctc gggatagtgt tttaaaacat cctgatgatt caaaatataa
     2341 aagactgcac cgcctacgat aattttgcat ttgatgtggc gaaatgtttt acggagcatc
     2401 atgagcagat ggtgaaattt ataaaatggg gaaagtgatg tgaccgacaa aaggatgtag
     2461 ttgtcctcag agttttccag ctcgctcatg ttttgcggcg tgagggtgtg tgtcgaaaag
     2521 gcaatgtcgg aatgagatcg aagatagctg agcaggtaca ggggattttc gttgggacgt
     2581 tttccctcgt agatgatggg gtagtatatc ttcatgtctt ctggcccctt tgaatttttt
     2641 tacaggcaac ctgtctgacg gttttttcga gtcgacggcg atttgagggg aattgttcca
     2701 tgaattctct caaaccaacc ttgacgccct tatcaaggaa aaaaccatct tgctcgctgg
     2761 catcgttttt gggttggcac ccttcaataa atttccaaaa gacctcttcc gaaatcgctg
     2821 agcagggttt gatggatctc tcaaaaagca tacgcagttg ttctttggtc cagatgccat
     2881 cgcgcagttt gatggggtca aaaatttcct ttacaactcc ctcatagcga aagggggcca
     2941 atgaggcgaa ttgttgctgg ttcatgacaa aggttttgcc atcttgcgag aagggcatga
     3001 aggctttttg ccagtcctca attgtgcatc ttttttcatc cacaaaaccg ctatagtata
     3061 aataatccac atcggcctgt ttgaaaacaa cgttttttcc atcggccttt ttcattgtgg
     3121 cccagatctg ctccatgact tcttttgatg atgacatgat tacccctgaa ataatgcaaa
     3181 atgtaaaatg taaaatgcaa agtgttaagt aaaaaagccc cgctttgcga ggcaaaaaac
     3241 acaaataaca atgtctatac ggatgtcaat tgactgccgg atgctgtgac cgatgcctgt
     3301 gcctctgaac tggcggattt cagaccgacc ggattgaggt cgtttttggt gacccctgct
     3361 tcaaagcttt tgagtttttt gttcgcgggt ggttgaagct cggtcgaggc gacggactga
     3421 tcgtctatgt tcaggatgtt gttgctctcg aagttgttga aagaccggct ctcgccggct
     3481 tccttcatgc cgcgagactc atcgtaataa gacccttctt gtgcgactgt acctgttgca
     3541 gaagcggtgg attctgttct ttgttgggta gtttgttcaa tcagcgaagg gtcggcttc
//
LOCUS       DATDUN010000317         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_222044, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000317 DATDUN010000000
VERSION     DATDUN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222044"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..563)
                     /gene="rpsM"
                     /locus_tag="VJC18_04355"
     CDS             complement(180..563)
                     /gene="rpsM"
                     /locus_tag="VJC18_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HLD44644.1"
                     /translation="MPRIAGVNIPGNKKIPYALQYIYGIGRTYAWDIVKKAEIDPNTR
                     ADDVSDDDMAKIRQMLENDYKVEGELRREVQLNVRRLMDFNTYRGTRHRKGLPVRGQR
                     SKTNARTVKGPRKTVAGKKKAPTAK"
     gene            complement(573..686)
                     /gene="rpmJ"
                     /locus_tag="VJC18_04360"
     CDS             complement(573..686)
                     /gene="rpmJ"
                     /locus_tag="VJC18_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HLD44645.1"
                     /translation="MKVRSSVKKICEKCNIIKRRGVVRVICSNPRHKQKQG"
     gene            complement(708..929)
                     /gene="infA"
                     /locus_tag="VJC18_04365"
     CDS             complement(708..929)
                     /gene="infA"
                     /locus_tag="VJC18_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HLD44646.1"
                     /translation="MAKEDVIEVEGTVVEPLPNAMFRVALPNGHKILAHISGKMRMHF
                     IKILPGDKVRVQLSPYDLNRGRIVFRDKT"
     gene            complement(951..1697)
                     /gene="map"
                     /locus_tag="VJC18_04370"
     CDS             complement(951..1697)
                     /gene="map"
                     /locus_tag="VJC18_04370"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HLD44647.1"
                     /translation="MIIIKSKDEIGFMRRAGRIVAGVLLKLGELIKPGVTTGELDRFA
                     EDYIRAHGGLPTFLGYRGFPGSLCTSLNCEVVHGIPGKRKLIEGDIISIDCGVTLDGY
                     IGDAARTYAVGQITADAARLIEGTEKALEAGMRQMKVGNRVGDVGCAVEGVAKQYGFG
                     VVRDFVGHGVGRSMHEEPQIPNFGNPGTGPRLKTGMVFAIEPMLNLGGYEVKVLEDKW
                     TVVTADGSLSAHAEHTVALTENGPEILTRV"
     gene            complement(1701..>2220)
                     /locus_tag="VJC18_04375"
     CDS             complement(1701..>2220)
                     /locus_tag="VJC18_04375"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HLD44648.1"
                     /translation="QTDLGKKAESFMLAGQLVPDAVVIGIVDERLLAPDCHKGYVLDG
                     FPRTEAQAEALSLTLENRGTKIHCVINIEVPDVLLVARLSGRRVCGTCGASYHIEYSP
                     SKTQGICDQCQNELIQRKDDNEETVQERLRVYHSQTKPLIAYYAKQNTLRPIDGTGEI
                     GFVKERIAKVLG"
BASE COUNT          528 a          660 c          418 g          614 t
ORIGIN      
        1 gcctcgggtg ccgccgttgt tgccgttgtt gccgttggcg cagttgccgc cgttgcttct
       61 gtttttgttt cgcttgcggt tgtattggtg ttggttgcca ttgtatcagt tcctgataac
      121 atcagacaaa tatcctgcac acacgcacaa tacccgtctg acagatttac aattattacc
      181 tacttggctg tcggtgcctt tttcttgccc gccacagtct ttctgggccc cttcactgtc
      241 ctggcgtttg ttttggaccg ttgtccgcga acaggtagcc ccttacgatg acgcgtacca
      301 cggtacgtgt taaaatccat caaacgacgt acattcaact gcacttctcg acgcaactcg
      361 ccctcgacct tgtaatcatt ttcgagcatc tgacgaattt tggccatatc atcgtcactc
      421 acatcatcgg cccgcgtgtt gggatcaatc tcggcctttt ttacgatatc ccaagcatag
      481 gttcgcccaa taccatagat gtactgtaaa gcgtagggta tttttttatt tcccggtatg
      541 ttcacaccag caattcgtgg catatgatct ccttatcctt gtttttgttt gtgtctcggg
      601 ttcgaacaaa tcaccctcac aacaccacga cgtttgatga tgttgcactt ttcacaaatc
      661 ttttttacag acgatctgac tttcataatc catccctgtc aaaaacgtta agttttatct
      721 ctaaacacta ttcttcccct gtttaaatcg tacggtgata actgaacacg caccttatca
      781 ccaggaagaa ttttgataaa atgcatacgc atcttacccg aaatgtgcgc caaaatttta
      841 tgcccgttcg gcaaagccac acgaaacatc gcattgggca acggctccac cacagttcct
      901 tccacttcaa taacatcctc tttagccata gttcacaccc taatcacttt tcacaccctg
      961 gttaatatct cgggtccatt ttctgtcaac gcaaccgtat gttcagcatg agcagagagc
     1021 gatccatcag ctgtcaccac agtccatttg tcttccaaaa ctttgacctc gtacccaccc
     1081 aaattgagca tgggttcgat cgcaaacacc atcccggtct ttaatcgcgg tccagtacca
     1141 gggttcccaa aatttgggat ctgtggttct tcgtgcatgc tccgacccac tccgtgtccc
     1201 acaaaatccc tcaccacacc aaacccatac tgtttggcga ccccttcgac agcacaccca
     1261 acatcaccaa cacgattccc aaccttcatt tgtcgcatcc ctgcttccag agccttttcg
     1321 gtcccctcga tcaacctggc cgcatctgcc gtgatctgac caacagcata ggttctggcc
     1381 gcgtcgccaa tatagccatc gagggtcaca ccacaatcaa tgctgatgat gtctccctca
     1441 atcaattttc tttttccggg aatcccatga acaacctcgc agttgagcga tgtacacaag
     1501 gatcctggaa aaccacgata acccaaaaaa gtgggtagcc ccccgtgcgc acgaatatag
     1561 tcctcggcaa aacgatcaag ctcacccgtg gtcacgcctg gtttgatcaa ctcgcccaat
     1621 tttaaaagaa cacccgctac gattcggccc gcccgccgca tgaaaccgat ttcatctttt
     1681 gatttgatga taatcacttt ctaacccaat accttggcaa tgcgttcctt gacaaagcca
     1741 atctcgcctg tgccatcaat gggacgcagc gtattttgtt ttgcataata cgcgatcagc
     1801 ggtttcgtct gagaatgata aactctcagc ctttcctgca cggtttcctc gttgtcatct
     1861 tttctctgga tcaattcatt ctgacactga tcacagattc cctgagtttt gcttggcgaa
     1921 tactcaatgt ggtaactggc accacaggtg ccacaaacac gtcttccgct caaacgtgcc
     1981 acgagcaaca catcgggcac ttcaatatta atcacgcaat gtattttcgt tccccggttc
     2041 tccagcgtta acgacaaagc ctctgcctgc gcctccgttc tggggaagcc atccaacaca
     2101 tagcctttat ggcaatcggg ggccaacaac cgctcatcca caatcccgat cacaacggca
     2161 tcaggaacca gttgcccggc cagcatgaac gactcggcct ttttgcccaa atcagtttga
//
LOCUS       DATDUN010000318         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_598058, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000318 DATDUN010000000
VERSION     DATDUN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598058"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VJC18_04380"
     CDS             <1..600
                     /locus_tag="VJC18_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44649.1"
                     /translation="GHSLLDLINNLLDLAKIESGKMLLNIEAFDFNDVITDIQSSLKP
                     LIEKKALKLRIHTQDKLPPMRADLFKMKQVLMNLLGNAIKFTENEGDIDIFVEHHQET
                     GELCHNIFSQQKLTDEIRLHSAFLVRVRDTGVGIRPEDLPHIFDLFRQADSSFTRKHE
                     GTGLGLALAKQIVTLHSGLINVNSQIGRGTEFRILIPQL"
     gene            641..1117
                     /gene="greA"
                     /locus_tag="VJC18_04385"
     CDS             641..1117
                     /gene="greA"
                     /locus_tag="VJC18_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007375825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HLD44650.1"
                     /translation="MDRIPMTPEGYQKLMHELKRIKSVERPKIVAEIEVAREHGDISE
                     NAEFHAAKDKQAVLTARMRAIETKLAQAEVIDPVMTKQIDKIVFGATVTLFDNETEGE
                     VLYKIVGDDETDIKGNKIGISSPLARGLIGKRLGDEVVIRTPKGTKEFEVLQVEYV"
     gene            1132..>2097
                     /locus_tag="VJC18_04390"
     CDS             1132..>2097
                     /locus_tag="VJC18_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HLD44651.1"
                     /translation="MIYLDHNATTPIDHRVLEAMMPYLTTVFGNPSSTTHAFGWDAVK
                     AVQTSREALAKCMGSTEADSIIFTAGATEANNLAILGAIDVAEKPHLITQKTEHDSVL
                     RTFAHLASRGCLVTYLDVDETGWLAPERLRSAMTDKTRLVSIMFANNEVGTVQEIRTL
                     AGIAHEWPHVLFHTDAAQAMGKVPIDVVTDDIDLMSFTAHKCYGPKGVGGLYVSSHCR
                     SLLKPIQFGGGQEHGLRPGTLNVAGIVGLAKAMVLGCEEQQCERQRLTQMRDHFIRDM
                     MHRLDGVTLNGHPTKRLFGNVNLSFAGVDAGKLTLSLKNVACSAGS"
BASE COUNT          561 a          479 c          560 g          497 t
ORIGIN      
        1 ggtcatagtt tgctcgatct catcaataac ctgttggatc tggccaagat cgaatcaggc
       61 aagatgttgc ttaacatcga agcgtttgat ttcaacgatg tgatcaccga cattcaatcc
      121 tcactaaaac cactcatcga aaaaaaggca ctgaagttgc gaattcacac acaggacaaa
      181 ttgccaccga tgagggcaga tctgttcaag atgaagcaag tcctcatgaa ccttttgggc
      241 aatgccatca agtttacaga aaacgagggg gatatcgata tttttgttga gcatcatcag
      301 gaaacaggcg agctttgcca caatattttt tcgcaacaga aattgaccga cgaaatacgc
      361 ctgcactctg cttttctggt tcgtgtgcgc gatacaggtg tggggatcag gccggaggac
      421 ctccctcata tttttgatct gttccgacag gcggattctt ctttcacgcg gaagcatgag
      481 ggcacgggct tgggcttggc gctggccaaa cagattgtca ccctgcactc gggcctcatt
      541 aacgtgaaca gccagatcgg cagaggaaca gaatttcgaa tattaatccc gcaactttag
      601 gagacgagag cgcatttttg ggcgttcttg acgtatattt atggatcgca ttcccatgac
      661 ccctgagggc tatcaaaaac tcatgcacga actcaaacga atcaagagcg tcgagagacc
      721 aaaaatcgtt gctgagatcg aagtggccag ggagcatggt gatatctctg agaacgccga
      781 atttcacgcc gcgaaggata aacaggccgt cctgacagcc cgcatgagag cgatcgaaac
      841 caaattggcc caagctgagg tgattgatcc cgtcatgacg aagcagatcg ataagattgt
      901 ttttggggcc acagtcactc tttttgacaa tgaaaccgaa ggggaagtgt tatacaaaat
      961 tgttggtgat gatgaaaccg atatcaaagg gaacaaaatt ggtatttcgt cacctttggc
     1021 cagggggctt attggaaagc ggttgggcga tgaagtggtg attcgaacac ccaagggcac
     1081 caaagaattt gaggtcctgc aagtggaata tgtctaaaga atgattgtgt catgatttat
     1141 ctcgatcaca acgctacgac cccgattgac cacagggtgc tggaggcgat gatgccttat
     1201 ttgaccacgg tgtttggcaa tccgtccagc acaacacatg cctttgggtg ggatgctgtc
     1261 aaggctgtgc agacgagcag agaggctctc gcgaagtgta tgggatctac agaggccgat
     1321 agtattattt ttacggccgg tgccaccgaa gcaaacaacc tggctattct gggagcgatc
     1381 gatgtcgccg aaaaaccgca tctcatcacg caaaagaccg aacacgacag tgtcctgcga
     1441 acatttgctc accttgcttc aagagggtgt ttggtgactt atcttgacgt tgacgaaacg
     1501 ggatggcttg ctccagaacg tctgcgctcg gccatgacag acaaaacgcg actcgtgagc
     1561 atcatgtttg ccaataacga ggtgggtacc gtgcaggaga tcagaacatt agctggcatc
     1621 gcgcatgaat ggccacatgt tttgtttcat accgacgccg cccaagccat gggaaaagtt
     1681 cccatcgatg tcgtgaccga cgacatcgac ctcatgagtt tcaccgcgca caaatgttat
     1741 ggacccaaag gggtaggggg attgtatgta tcctcccact gtcgatcact gttaaaaccg
     1801 atccagtttg gcggtgggca ggagcacgga ttgcgcccgg gaacacttaa tgtggcgggt
     1861 atcgtgggac tagccaaggc gatggtgctt ggttgtgagg aacagcagtg cgagaggcag
     1921 agactaacac aaatgagaga tcatttcatt cgggatatga tgcaccgact cgatggcgtg
     1981 acgctcaatg gtcatcccac caagaggctt tttggtaacg tcaatttgtc ttttgcgggt
     2041 gtcgatgccg gtaaattgac attgtcactt aaaaacgttg cctgttcggc gggatcg
//
LOCUS       DATDUN010000319         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_598171, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000319 DATDUN010000000
VERSION     DATDUN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598171"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VJC18_04395"
     CDS             <1..175
                     /locus_tag="VJC18_04395"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLD44652.1"
                     /translation="NHVNNIPIIIPCHRVIGKNGKLTGYAGGLHIKKWLLEHEKGAKN
                     SCLCYQGPEAAIY"
     gene            complement(126..1055)
                     /locus_tag="VJC18_04400"
     CDS             complement(126..1055)
                     /locus_tag="VJC18_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44653.1"
                     /translation="MNRNNDIITLFKIVFCLLALSACSGASDSYVDDGGDSVEVPALT
                     CSYDNDCDGDGLYTTCDTDDGDAEIVAIKEGCDEDGDGFVDTSCAAYSDDNGDGFITA
                     DERDINCDVCAGIADPDQTDSNDDGIGDACDVLLNINESAAPPAEQDPADTSVLTETA
                     MLSVTEHSVLITRGGQFNISFSLDNDSSVQSFLVDVVLLGETKTMIKAASLTLSPVVN
                     VKQLGLCGIYYEACVYLDEQEIESPSGQEAEYGLSFTVPTDAAAGTYTVQLRIMTVAA
                     DGAVTYYDRESGLGEVTISKLQLLVPDSKGKNF"
     gene            1275..>2043
                     /locus_tag="VJC18_04405"
     CDS             1275..>2043
                     /locus_tag="VJC18_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HLD44654.1"
                     /translation="MKLTNLLICFLVVIINLAVWLYINKPYHPVNSHYPLNGMCFNPF
                     QKNQTPYDGVAFNAQDMEKDLAVLSDKTKTVRVYTATNGMDQLPKLAEKFGLNIVLTA
                     WLDSRAVSNNNQTEVNSAIQLTGRNRNIKRLLIGNETQLHKSVPREELITYLDEARRR
                     LKTPVSTAESWDYWLNNPDLADHVDFIAIHVLPYWVGIPIEEAADYVLGKYWALKERF
                     PEKKIIITEAGWPSEGPQRGSAVPSLINQAMFIRSFVR"
BASE COUNT          562 a          593 c          439 g          449 t
ORIGIN      
        1 caatcatgtg aacaacatcc ccatcatcat cccctgccac cgggtgatcg gtaaaaatgg
       61 aaaactgaca ggttacgccg gcggtctgca cataaaaaaa tggttattgg aacatgaaaa
      121 aggagctaaa aattcttgcc tttgctatca gggaccagaa gctgcaattt actgatcgtc
      181 acctcaccca gaccactctc ccgatcataa taggttacgg ccccgtcagc agcaacagtc
      241 atgattctga gttgcaccgt gtaggttcct gccgcagcat ctgtggggac agtgaaactc
      301 agcccatact ctgcctcttg acccgatgga gactcgatct cctgttcgtc aagatacaca
      361 cacgcctcat agtagatccc acacaaacca agctgtttaa cattcacaac aggcgacaat
      421 gtgagcgacg ctgccttgat cattgttttg gtttccccca acaacaccac atccaccaga
      481 aaggactgca cactggaatc attgtctaag gaaaaactaa tgttaaactg tccccctctg
      541 gtgatcaaca cggaatgttc tgtcacactc agcattgccg tttcggtcag tactgacgta
      601 tcagctggat cctgctcagc gggaggagcg gcagactcgt tgatgttcaa gagcacatcg
      661 cacgcatcac ctatgccgtc atcattggaa tctgtctgat cgggatcggc aatgcccgcg
      721 cacacgtcac agttgatgtc ccgctcatcg gctgtgatga acccatcacc attgtcgtcg
      781 gaataagccg cacaggacgt gtccacaaaa ccatcgccat cctcgtcgca accttccttg
      841 atcgccacaa tctcagcatc accatcgtcc gtatcgcagg tggtgtacaa cccatctccg
      901 tcgcagtcgt tatcgtagga acaggtgagc gcgggaacct ctacgctatc accgccatca
      961 tcgacataac tatcagaggc tccggaacag gccgacaggg ccaacagaca aaaaacaatc
     1021 ttaaaaagcg tgatgatgtc attattgcgg ttcatattgg tgcgactccc ttctgaccca
     1081 ttatcggcaa aatttcaatg aagttgtgag gttttacacc cccttctcca atgatgctga
     1141 atcaatagta ttattagccg gttaggaaaa aaatgccccg catcacccta ttgacgacag
     1201 ggtaaaataa gaatagtgag ccgctaagaa aaatcacctc cgtcgagagt ctatccaacg
     1261 ccgatactaa ctctatgaaa ctcacaaatc ttctgatctg ttttctggtc gtcatcatca
     1321 atttggccgt ctggctatac atcaacaagc cctatcaccc cgtaaattcc cactacccgc
     1381 tgaacgggat gtgcttcaac cccttccaaa aaaaccaaac tccctatgat ggtgtggcct
     1441 ttaacgcgca agacatggag aaagatctcg ctgtacttag cgacaagacc aaaaccgtac
     1501 gggtgtacac agccaccaac ggcatggacc aactccccaa gctggccgaa aaatttggtt
     1561 tgaacattgt gctcacagcc tggctcgata gtcgcgcggt ctcaaataac aatcagaccg
     1621 aggtcaactc cgccatccaa ctgacaggcc gcaaccgcaa catcaagcgc ctcctgattg
     1681 gcaacgaaac acaactgcac aaatccgtgc ccagggaaga actgatcacc tacctcgatg
     1741 aggccagacg ccgtctgaaa acccccgtgt ccacagctga atcgtgggac tactggctca
     1801 acaatcccga tctggccgat cacgtcgatt tcatcgccat ccacgtcctg ccctactggg
     1861 tgggcattcc cattgaggaa gccgccgact atgtgctggg aaaatactgg gccctcaaag
     1921 aaagattccc cgaaaaaaaa atcatcatta ctgaagcggg ctggcccagc gagggcccac
     1981 aaagggggtc ggccgtaccg agtttgatca atcaggccat gttcatccgt tcgtttgtcc
     2041 gtc
//
LOCUS       DATDUN010000320         1136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_306229, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000320 DATDUN010000000
VERSION     DATDUN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306229"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VJC18_04410"
     CDS             complement(<1..631)
                     /locus_tag="VJC18_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLD44655.1"
                     /translation="MLGKNFKAGIFVLATILLLVYMILRINQGSLFKTGGYPIFLEIE
                     SAVGIDTRTQVQIAGVGVGEVKSITLLPTNKARVELNIRKDIVISENAMGLIKVTGVL
                     GDAYIEIYQPGQASAPMRSGSTIKQVTSFGDFNSVTGKISAIADDVKAVTEQMKKLMA
                     GDESVFDKTVKNIESITGSLERLTQKNEDNVNAIISNFKALAENLNQVVA"
     gene            complement(654..>1136)
                     /locus_tag="VJC18_04415"
     CDS             complement(654..>1136)
                     /locus_tag="VJC18_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLD44656.1"
                     /translation="MTVFENIAFPLVEHTKKPFLEVTKDVSALAAMVGLSRLALSKFP
                     SELSGGMRKRVGLARAIALKPKILLYDEPTTGLDPIMTDVVNHLITDTQKQLHITSVV
                     ISHDIQSSFEIGDKIAMLQNGKIVLEGDAGEFQKTQNTVVKNFLSGKACQEEYTDTSV
                     "
BASE COUNT          265 a          306 c          254 g          311 t
ORIGIN      
        1 tggccaccac ctgattcagg ttttcggcca gcgccttaaa attactgatg atcgcgttga
       61 cattgtcttc gttcttttgc gtgagtctct ccaacgaacc cgtaatgctc tcgatatttt
      121 tcaccgtttt gtcgaaaacg gattcgtcac cagccatgag ctttttcatt tgctctgtca
      181 cagccttgac atcatcggca atcgccgaaa ttttacccgt cacgctgttg aaatcaccaa
      241 aactcgtcac ttgtttgatc gtggaacctg acctcatggg agcggaggcc tggccgggct
      301 ggtaaatttc gatgtaggca tcacccagca cacccgtgac tttgatgagt cccatcgcat
      361 tttccgaaat aacgatatct ttgcgaatgt tcagttcgac tcgtgccttg ttggttggca
      421 agagcgtgat gcttttgact tcgcccacac ccacaccggc tatctggacc tgtgttctgg
      481 tatcgatccc aacggcggac tcgatttcga gaaaaatggg gtaaccacct gtcttgaaca
      541 acgagccctg gttgattctg aggatcatgt agacgagcaa cagaatcgtc gccagaacaa
      601 aaatacccgc tttgaaattc ttaccaagca tcaaaatctc ctgactgaca gactcaaaca
      661 gatgtgtcag tgtactcttc ctggcaggct ttaccactta ggaagttttt gacaactgta
      721 ttctgggtct tttgaaactc accagcatcc ccttccagca caatcttccc gttctgcaac
      781 atggcgattt tgtcgccaat ttcaaacgag gattgaatgt catgactgat caccacagac
      841 gtgatgtgca gttgcttctg cgtatctgtg atgaggtgat tcaccacatc ggtcatgatg
      901 ggatccagcc cggttgtggg ttcgtcatac agcaggatct ttggttttaa ggcaatcgcc
      961 ctggccaacc ccaccctctt cctcatccca cccgaaagct cagagggaaa cttggacagg
     1021 gccaatcgac tgagtcccac catggccgcc aatgcgctca catccttggt cacctcaagg
     1081 aacggctttt ttgtgtgttc caccagaggg aatgcgatgt tttcgaaaac ggtcat
//
LOCUS       DATDUN010000321         4750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_140922, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000321 DATDUN010000000
VERSION     DATDUN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140922"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VJC18_04420"
     CDS             complement(<1..286)
                     /locus_tag="VJC18_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44657.1"
                     /translation="MKIFRIKIVALGLGVILVALLMNMCGGSSATDGDDDTDGDGDSS
                     VTVSFAPSATASAISRSATVSATFSAAITEPLSWYNLVKLQKNGTGATLCS"
     gene            complement(370..897)
                     /locus_tag="VJC18_04425"
     CDS             complement(370..897)
                     /locus_tag="VJC18_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44658.1"
                     /translation="MKRVKWILIFLFSCLACLGGGTVWAATSCENTTTTCEDMCAHVY
                     DTCAVSLIDSGGSARSEAECVSACASTDPAVISCLASVSCTAEAVDNCLISATSTGTS
                     TDDSSYIGGGGVSDGETTTSGGACGEITDCNSCISNCRCRYDGTVAASQCCTGCGCEC
                     ELYCMPTEWWIATCI"
     gene            1029..1238
                     /locus_tag="VJC18_04430"
     CDS             1029..1238
                     /locus_tag="VJC18_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44659.1"
                     /translation="MSKKISTLVNEDVWDEFKGLSRQHHQNLTGMLNEAIREYIQRRK
                     LRPKFIRHMENSIADNNTLGRLLAK"
     gene            1235..1624
                     /locus_tag="VJC18_04435"
     CDS             1235..1624
                     /locus_tag="VJC18_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HLD44660.1"
                     /translation="MILFPSLDEALELYQKLIDEFGGSHGIRDLGLLESALYRPQTGY
                     YEDLFSMAAALMESFLINHAFVDGNKRMAFFITDIFLRMNGYKIKTIPKTGHKFIINL
                     THSSSQRFETIVTWLETHCVSLRDNDA"
     gene            complement(1604..2302)
                     /locus_tag="VJC18_04440"
     CDS             complement(1604..2302)
                     /locus_tag="VJC18_04440"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HLD44661.1"
                     /translation="MIGLGTLVNVVAIIVCSLIGMGLGHKLPERVHDLLTKAMGLVIV
                     GIGLKMFLATQHIVIVLLSLVLGALVGELLGIEAGLETCGHKLKKMTRSKSSHFLEGF
                     VTASLIYCVGPMAIIGAMTDGMGAGHEILFAKSILDGTISITLATTLGVGVLFSFIPV
                     LLYQGGFTVLAFFLGHFFAQPLIAELTATGGLLIAGIGINILGQLTDRNRIAVGNLLP
                     AIVIAPVLLSIIIS"
     gene            complement(2420..3208)
                     /locus_tag="VJC18_04445"
     CDS             complement(2420..3208)
                     /locus_tag="VJC18_04445"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLD44662.1"
                     /translation="MIFENAIQAWRQRCPWREQRHVEQDLILHSMLTLIYSDPIIAEK
                     LAFRGGTCLNKLYLDRPFRYSEDLDFVQIKAEPIGPIAERIKIKLTEIFSDKPQWEAK
                     KGGFKIFFSFLPEDSPYKQKIKIEINTREHLAVKGFVKKKIVLDSLWRSGEADVTTFV
                     LEELLATKLRALYQRKKGRDLFDLWVSQKLEPDYKEVVAIFGAYMKNEGHRVSREQFM
                     ENLQNKMQLPQFLNDMAPLIRPGTGYDPKHAWKFVLGKLLSLLA"
     gene            complement(3198..3995)
                     /locus_tag="VJC18_04450"
     CDS             complement(3198..3995)
                     /locus_tag="VJC18_04450"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HLD44663.1"
                     /translation="MYKKKGENLLDTLLAEAKTCFSLADAQQKLGLPKNLVVKILSTL
                     RKKNKIIKLSKGYYAYFPPAEKKFGFNILSALDQVMKHRELPYFVGLLSAADYYGAAH
                     HKPQVMQVVIPRQISFRKAKDLGISLHVQKQFPKKGLVRIKTPQGYLWYSSPEYLVLN
                     MLEYEQACGGIENVVAIIKEMLPLLNDDALKQMALEYPVAAVVQRLGYVLEKMNADKA
                     LLKILIQVMHKRGASVAKLSAVLPKKGTFESKWKIIQNTRLEMNDDI"
     gene            complement(4169..>4750)
                     /locus_tag="VJC18_04455"
     CDS             complement(4169..>4750)
                     /locus_tag="VJC18_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44664.1"
                     /translation="REHQGIRGEAIDDYCVEPKPYDVERLYYRFVDGAHQLVADEIAG
                     HLLHYDALFIAMLVSLIEGRWIGLPTEAEFEYVARAVLGEQGWMPKADKARDIRLSEM
                     AIQVNLNRSIRDLEPDILGFYGLLGVVSQHCADTMCQYSSDTQADPIGRGRNNERISR
                     GAGKLRRPNHVHARFMNSPDDAYSSNAIRLVGQ"
BASE COUNT         1298 a         1214 c         1012 g         1226 t
ORIGIN      
        1 aagaacacag ggtggccccc gtgccgttct tttgcagttt gaccagattg taccaactca
       61 aaggctctgt gatggccgcc gaaaaagtgg cggagaccgt tgcagaacgc gagatcgctg
      121 acgcggtcgc tgagggggca aacgatacgg tgaccgagga gtctccgtca ccatcagtgt
      181 cgtcatcccc atcggtcgca ctcgaaccac cacacatgtt catcaacaac gcaaccaaga
      241 tcacacccag acccaaagcc actatcttga ttctaaatat tttcataata acctcgcact
      301 tcgctatctc tgccatattt caatagtccc caccgttcgc cctgcgcctt acgccttaag
      361 ccattcgcct tatatacacg tcgcaatcca ccattcggtg ggcatgcaat acaactcaca
      421 ctcacacccg caaccggtac aacattgact ggccgcgact gtcccgtcat aacggcatcg
      481 acaattcgaa atgcaggaat tgcagtcggt gatttcgcca cacgccccgc cgctcgtcgt
      541 ggtctcgcca tcggacacac ccccaccgcc aatgtaggaa ctgtcatctg tagaagtccc
      601 cgtgctggtg gcgctgatca gacaattatc aaccgcctcg gccgtacagc taacggaggc
      661 gagacaagaa atgacagcgg gatcagtcga ggcacaggcg gaaacacact cggcctccga
      721 tcgcgcggac ccgcccgaat caatcagact cactgcgcaa gtatcgtaaa catgggcgca
      781 catgtcctcg caggtcgtcg tggtattttc gcacgacgtg gccgcccaaa cagtcccgcc
      841 cccaagacag gccagacagc taaaaagaaa aatcaaaatc catttcactc gtttcatcct
      901 gcccctccct ctttatttct tcattgttta cgtatagata acaaagtacg tgggatgtaa
      961 agcgcaaatt tttccagtat gatcttgata tatcttgaat tatctagatc attgtaggat
     1021 aatgacccat gagtaaaaaa atcagcacat tggtcaatga agatgtttgg gatgagttta
     1081 aaggcttatc cagacaacat caccaaaatc tgactggcat gcttaacgaa gccatcagag
     1141 agtacattca gcgccgcaaa ctacgtccca aattcatcag acacatggaa aactccatag
     1201 ccgacaacaa tacgttggga cgattactcg caaaatgata ctcttcccct ccctagacga
     1261 agccctcgaa ctgtatcaga agctcatcga tgaattcggc ggcagtcatg gaatcagaga
     1321 cctgggtctg ctggagagcg ccctgtatcg cccacaaaca gggtactacg aagatctatt
     1381 ttccatggcg gccgctctga tggagtcctt cctcatcaac cacgcttttg ttgatggcaa
     1441 taagcgcatg gcatttttca tcaccgatat ttttctgcgg atgaacgggt acaaaataaa
     1501 aacgattcca aagacagggc acaagttcat cataaacctc acccactcta gctctcagcg
     1561 ttttgagaca attgtcactt ggctggaaac gcattgcgtc tctttacgag ataatgatgc
     1621 ttaacaacac cggtgcgatg acgatggcag gcaacagatt tcccacggca atgcggttcc
     1681 tgtctgtcaa ttgccccaga atgttgatcc ctatgcctgc aatcaacaaa cctcctgtgg
     1741 cggtgagttc ggcaatcagc ggctgggcaa aaaaatgccc cagaaaaaaa gccagcacgg
     1801 taaatccccc ctgataaagc aaaacaggaa taaacgaaaa aagaacaccc acacccaggg
     1861 tcgtcgcaag cgtgatcgag atggtgccat cgagaatgga tttggcaaaa aggatctcgt
     1921 gcccagcgcc catgccatct gtcatggcgc cgatgatcgc catggggccc acacagtata
     1981 tgagactagc ggtgacaaag ccctccagaa aatgggagct tttggaccgg gtcatttttt
     2041 ttagtttgtg accacatgtc tcaagtcccg cctcaatgcc cagcagttct cccaccaaag
     2101 cccccagcac caggctcagg agtacaatga cgatgtgctg ggtggccaga aacatcttga
     2161 ggccgatgcc cacaatgacg aggcccatgg ccttggtcaa aagatcgtgc acacgttcag
     2221 gcagtttgtg gccaagaccc atgccgatga gtgagcacac aataatcgcc acgacattaa
     2281 ccagtgttcc cagtccaatc atgtgagcat cttattacaa aaaatgtaag aaatgaattt
     2341 aaatattttc acccgtgtcc tgcaggacac ggtttgtgcg gcagggaagc aataaatctt
     2401 ttttcaatcg ttggttcacc tacgcaagta acgaaagcaa ttttcccaaa acaaatttcc
     2461 aagcatgttt tgggtcataa ccagtcccag gtctgatcag tggtgccata tcgttcaaaa
     2521 attgtggtaa ttgcattttg ttttgcaggt tttccatgaa ctgttctcgt gaaactcggt
     2581 gtccttcatt tttcatgtat gcaccaaaaa tggcaaccac ttctttgtaa tctggttcga
     2641 gcttctgact gacccaaaga tcaaaaaggt ctcgtccttt ttttcgttga taaagggcac
     2701 gcaacttcgt ggccaataat tcctcgagca caaaagttgt cacatcagct tctcctgaac
     2761 gccacaacga atccaacaca attttctttt tgacaaaacc ttttacggcc aaatgttctc
     2821 tggtattgat ttctattttg attttttgct tgtacggcga atcttcaggc aaaaacgaaa
     2881 aaaatatttt gaaccctccc ttttttgctt cccattgagg tttgtcagaa aaaatttctg
     2941 tgagcttgat ttttatacgt tcggcgatcg gtccgatggg ctcagccttg atctgtacaa
     3001 aatccagatc ttcgctatag cgaaagggtc tgtccaaata gagtttattc aggcaggttc
     3061 caccacgaaa agccaatttt tcagcaatga tagggtccga ataaatgagc gtgagcatcg
     3121 agtggagaat caaatcctgt tccacgtgtc tttgttcacg ccaagggcaa cgctgacgcc
     3181 acgcttgaat ggcgttttca aatatcatcg ttcatctcca gtctggtgtt ttgaatgatc
     3241 ttccactttg attcaaatgt ccctttcttt ggcaatactg ctgacaattt agcgacagaa
     3301 gctccccgtt tatgcatcac ttgtatcaag attttcaaca gagctttatc ggcattcatt
     3361 ttttccagaa cataacccag tctttgcaca acagccgcca cggggtattc caaggccatt
     3421 tgtttcaacg catcatcatt cagcagtggc aacatttctt tgatgatggc gacaacattc
     3481 tctatcccac cgcaagcctg ttcgtattcg agcatattca atacgaggta ttcgggcgag
     3541 gaataccaga gatatccctg aggagttttg atccgaacca gtcccttttt gggaaattgt
     3601 ttctggacgt gtaatgatat tcccaaatct ttagcctttc gaaaactgat ctgtcggggg
     3661 atcacaacct gcatgacttg tggtttatgg tgtgcggccc cgtagtagtc cgctgccgag
     3721 agcaatccaa caaaataggg tagctcccga tgtttcatga cttgatcaag agcgctcagg
     3781 atattaaaac caaacttttt ttctgcaggg ggaaaatagg cataataccc ctttgaaagt
     3841 ttgatgatct tgtttttttt cctcagggtt gacagaattt ttaccacgag attttttgga
     3901 agtcccaatt tttgttgagc atctgcgagc gaaaaacagg tttttgcttc tgccagcaag
     3961 gtgtcgagca agttctcccc ttttttctta tacataccat atctaccgtt tatcatgagt
     4021 tattatatat acaggaaatg tgaatggtca agacatcatt aggaattttt cacttgagaa
     4081 taaaaaacgg agtatcatgc tggggttttt agagatgtct atagatttag gtaaaaggaa
     4141 atgataaaaa aaatgggccg ttcccgggtc actgtccaac aagacggata gcattggaag
     4201 aataggcatc atccggagaa ttcataaaac gagcatgcac atgatttggt cgtcgtaact
     4261 tccccgctcc tcgcgagata cgttcgttat ttcttccccg acctatcgga tcggcttgtg
     4321 tgtctgaact gtattgacac attgtatctg cacagtgttg agatactaca ccaagcagtc
     4381 catagaaacc gaggatatcc ggctccaggt ctctgatcga acgattcaag tttacttgga
     4441 ttgccatctc cgataacctg atgtcgcgag ctttgtcggc cttcggcatc catccttgct
     4501 ctccaagcac agcacgggcc acatattcaa actcagcttc agtcggcaga ccgatccagc
     4561 gaccctctat caatgatacc aacatcgcaa taaacaaggc gtcgtagtgt aaaaggtgcc
     4621 cggcaatctc atcagcaacc aactggtgtg cgccatcgac aaaacgataa tagagacgct
     4681 ccacatcata aggcttcggt tcaacgcaat aatcatctat cgcttctcct cttataccct
     4741 gatgctcgcg
//
LOCUS       DATDUN010000322         1094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_389706, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000322 DATDUN010000000
VERSION     DATDUN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389706"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VJC18_04460"
     CDS             <1..300
                     /locus_tag="VJC18_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44665.1"
                     /translation="GDGEYSLFSVNTDLSSLAPEGTDTFCLVSVDGGLPTQTEEVNCS
                     ALETAYAWADEEFPFELDPEIPVVFEEKEFFESTDDDLIASDGSNFSIPQYLTVE"
     gene            368..892
                     /locus_tag="VJC18_04465"
     CDS             368..892
                     /locus_tag="VJC18_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44666.1"
                     /translation="MKTKYFFIVLFTIIFFNCTCFGKDIAKYACEQDADCMNSCSQGA
                     VNATWYSKEFAIKTECLDGCDGPATSSVRCVNKICATFQFGKYAAHCSRGQNIQPPYP
                     LELTKFSCDRNTDCLMHCAHGAVNRSWNNTNGNKDACEDGCVGEGTYPRCEKHICTAY
                     RDDKPYAECTKVME"
BASE COUNT          273 a          251 c          288 g          282 t
ORIGIN      
        1 ggtgacggtg agtattccct gttttcggtc aacaccgacc tgtcttcgct ggcacccgaa
       61 ggcacagata cgttctgtct ggtgagtgtc gatggcggat tgcccacaca gaccgaagag
      121 gtcaactgtt ctgcgctgga aacggcgtac gcctgggcgg atgaagaatt tccctttgaa
      181 cttgatccgg aaatccctgt ggtgttcgaa gaaaaggaat tctttgaaag cacagacgat
      241 gatctgatcg ccagtgatgg gagcaatttc agtatccctc agtacctgac tgttgaataa
      301 ttcgggagcg cacaacaaag actgcccgcg tggcatctgt caccacagcg ggcagtcgca
      361 ttattttatg aaaacaaaat atttttttat tgttctattc acaatcattt tttttaactg
      421 cacttgcttt ggcaaggaca ttgccaaata cgcgtgtgag caggatgcgg actgcatgaa
      481 ctcctgttcg caaggggcgg tcaatgccac ctggtacagc aaggagtttg ccatcaaaac
      541 cgagtgtctg gacggctgtg acggaccagc cacgtctagc gtgcgttgtg tgaacaagat
      601 ttgcgccacg tttcagtttg ggaaatatgc ggcccattgt tcacgtgggc aaaatataca
      661 accgccttat cccctggagt tgaccaaatt ctcctgtgac cgcaatacag actgcctcat
      721 gcattgtgcg cacggcgcgg tgaatagatc atggaataac actaatggca acaaggatgc
      781 ctgtgaagat gggtgcgttg gtgagggcac atacccacgt tgcgaaaagc acatctgcac
      841 ggcctatcgt gatgataaac cgtacgcaga gtgcacgaaa gtgatggaat agaactttga
      901 gtgtaatcta tcagtcttaa ggtgggaaag gtacagaatc attacctttg agtctcctat
      961 ttcagtctct tcttgatggc gtcttccagc gtatgcgcat cgtggttttt gtactcatac
     1021 cccacacgca tggtgggctt gttgatggtc acgaggctgg tcaaattgat tggcgttgcg
     1081 aacaagacga gatc
//
LOCUS       DATDUN010000323         3282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_181720, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000323 DATDUN010000000
VERSION     DATDUN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181720"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VJC18_04470"
     CDS             <1..440
                     /locus_tag="VJC18_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44667.1"
                     /translation="DSLVTTNDILMNATNYPLWLLSGLILLRGASASRALSESGNSLI
                     QAIREKGPDRITTAKQFAISLSSTTGSALMLCCVLFSHPVIAYQGPVLKASAMTFLFL
                     SAGLDFGAAIKRQTWKDGFIAGLKRLKKNGFSRHQSPSITETK"
     gene            complement(442..2424)
                     /locus_tag="VJC18_04475"
     CDS             complement(442..2424)
                     /locus_tag="VJC18_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HLD44668.1"
                     /translation="MSINAISVTKARMTMAFITRLRVVGFFAAAVLFVALIALPLFYR
                     HINRVFEIEVDAIMAKCNSEWEVTAQFSQAGYDLLTKPYISGLQIKQNKSQGFVPVVK
                     IPRISFSYRVTLGARPHLVITQIDLNRPEVLFTLDSIPQDKDPQILVHQSKQALKGLF
                     EKLSQFHRSLSSDFVSLAPDVRVTWNDALVKITDRISEEPYQAVQHSFEGLSGTSTFF
                     LPQQEIQVVVSSTRHPEQKIIAALNKQNDDFVLKLKAEQIILNEFARYLPDVVKTTGQ
                     SSLSGKLSMAPIDLSQSSHILFDFSIQKIGLEHWRLADQELSDLSFKTKGDLDWLASE
                     QKVRVHKTSLARDDVTLSFVGDLEYGAKKSINTTLTLESTPIQKLLNALPKEFIPVIK
                     GAKVAGDISVKVDVALDMARPRDMVFEPKINILGFDLVRAPEMADVLKLKQAFEHTVR
                     LSGTDEKTFAVGPQNRQFVSYGRLGNNIIKGVLTCEDGSFFRHEGFQLKHIRESIIQN
                     IKEKRFVRGASTITMQLAKNLFLSGQKTLSRKFQEMLIAYALEQELDKKRLLEIYMNV
                     IEWGPGIYGVGQASRHYFHKAPAQLSVLEAAYLGSIIANPKKYYYMYRRGEVTDYWAD
                     YLRVIVSKMSLAGAALEDVLLDDSYKPRFGWVASKI"
     gene            2678..>3282
                     /locus_tag="VJC18_04480"
     CDS             2678..>3282
                     /locus_tag="VJC18_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44669.1"
                     /translation="MNKEKLEYGAYQVAINCLRTQPNDQVCIITDDVTMHIGKAIESQ
                     FLKVTKKVKLFRMEDFGDRPDDGSNPLKFPDEIANYLKNSQVSVYAGRGKKGELPSFR
                     HPMTILTDEYNIRHGHMPNVNDLLMETGMCADYAKIKENNAKVMKLVQGTKHAQVTTD
                     AGTNIEVTLDPNWKWINCDGNIQPGHWSNLPDGEVFTCAKSV"
BASE COUNT          873 a          866 c          725 g          818 t
ORIGIN      
        1 ccgattctct ggtcaccacc aacgacatcc tgatgaacgc caccaattat cccttgtggt
       61 tattatcagg actaattcta ctacgagggg cgagcgcgtc acgggccttg tctgaatccg
      121 gcaattcact gatccaagcc attcgtgaaa aggggccaga tagaataacg actgccaaac
      181 agtttgcaat ttctctctcc agcaccacag gttctgcgct gatgctgtgc tgtgttctgt
      241 tctcccaccc cgtcattgcc tatcaaggac ccgtcctcaa ggcgtcggcc atgacgtttt
      301 tatttttgtc ggcaggactg gattttggtg cggccatcaa acgacaaacg tggaaggacg
      361 gatttatcgc gggtctgaag agactcaaaa aaaacgggtt ctcacgccac cagtccccat
      421 cgatcacaga aacaaaataa atcaaatctt tgacgctacc cagccaaacc tcggtttata
      481 ggaatcatca agcaggacat cttccagcgc ggcccctgcc aagctcatct tgctcacgat
      541 cacacgcagg taatctgccc agtagtccgt cacctcacca cgtcgataca tgtaataata
      601 ttttttgggg ttggcgatga tggaccccag atacgccgct tccaaaacgg agagttgggc
      661 cggtgctttg tgaaaataat gccgtgaggc ctgccctacc ccataaattc ccggccccca
      721 ttcaatgacg ttcatgtaga tttccaacaa tcgtttttta tcgagctcct gttccagtgc
      781 gtaggcaatc agcatttcct gaaatttacg cgaaagtgtt ttttgtccag acaaaaacaa
      841 attttttgcc agttgcatgg tgatcgtcga agcccctctg acgaaacgct tttccttgat
      901 gttctgtatg atcgattcgc gaatgtgttt gagttgaaaa ccctcgtgtc tgaaaaaact
      961 gccatcctca caggtgagca cccccttgat gatgttgttt cccaagcgcc cataagacac
     1021 gaactgcctg ttttggggac ctaccgcgaa ggtcttttcg tcggtccccg acaatctgac
     1081 tgtatgttca aaggcctgct tcaacttgag aacatcggcc atttctgggg ccctaaccaa
     1141 atcgaaaccc aaaatgttaa tcttcggctc aaaaaccata tcccgaggac gggccatatc
     1201 caacgcaaca tccactttga ccgaaatatc cccagccact ttggcgccct tgatcacggg
     1261 aataaactcc ttaggcaagg cattgagcag tttttgaatg ggcgtgcttt cgagggtcag
     1321 tgtggtgttg atacttttct tcgcaccgta ttccaaatct cccacaaacg ataatgtcac
     1381 gtcgtcacgg gcaagggagg ttttatgcac acgcaccttt tgctcagaag ccaaccaatc
     1441 caaatctccc ttcgttttaa aactcaaatc ggataattcc tgatccgcca gccgccagtg
     1501 ttccaaccca attttttgaa tcgaaaaatc aaagaggatg tgacttgatt gtgacaagtc
     1561 gattggcgcc atgctcaatt tgccgctcaa ggagctctgc cccgtggttt tgacgacgtc
     1621 cggaagataa cgcgcaaatt cgttcaagat gatctgctca gccttgagct ttaatacaaa
     1681 atcatcattc tgtttattca gcgcggcgat tattttttgc tccgggtgac gggtggaaga
     1741 gacgacaacc tgaatctcct gttgtggcag aaaaaacgta ctggttccgg ataggccttc
     1801 aaagctgtgt tgcacggctt gataaggttc ttcggagatt ctgtctgtaa ttttgaccag
     1861 ggcatcgttc caggttaccc ggacatcagg ggccagggac acaaaatcag aagacaggga
     1921 tcgatgaaac tgggagagtt tttcaaacag acctttcaaa gcctgtttcg attggtggac
     1981 caggatttgc ggatctttgt cttgagggat cgaatccagc gtaaaaagaa cttcgggacg
     2041 attcaaatca atctgtgtga tcaccagatg ggggcgggca cccagtgtaa cccgatagga
     2101 aaatgaaatt ctgggaattt taacaaccgg cacaaaaccc tgggacttgt tttgtttgat
     2161 ctgcaacccg ctgatgtacg gctttgtgag caaatcgtat ccagcctggc taaactgggc
     2221 cgtgacctcc cattcgctgt tgcacttagc catgatggca tcaacctcga tctcaaaaac
     2281 acgattgatg tggcgataaa acaagggcaa ggcaatgagt gctacaaaaa gtactgccgc
     2341 cgcaaaaaaa cccacaaccc gcaatctggt gatgaacgcc atcgtcatgc gcgctttagt
     2401 aacagaaata gcattgatac tcaacacccc tttccaacta tcaactctca attttcaact
     2461 gctcatattg gcaattgact gttgccaatt gatagttgtt gcctcccatt accaattatc
     2521 catgacccat gacctatgac ctattaccca tataatatgc ttgatatttg gctggtatcg
     2581 tggcactttt tactccgctg tcattgctcg cgatggccat tctatatttt cagaatgttt
     2641 tcgtaacgct acgtccagaa acaaaggaga tattaccatg aacaaagaaa aactcgaata
     2701 cggtgcttat caagtggcca tcaactgtct cagaacccag cctaacgatc aagtttgcat
     2761 catcaccgat gatgttacca tgcacatcgg caaggccatc gaaagccagt ttttaaaagt
     2821 caccaaaaag gtcaaactgt tccgcatgga agactttggt gatcgtcccg atgacggttc
     2881 caatccccta aagtttcccg acgagattgc caactacctt aagaattcac aagtgagtgt
     2941 gtacgctggt cgcggcaaaa agggtgagct ccccagcttt cgtcacccca tgaccatcct
     3001 gaccgacgag tataacattc gccacggcca catgcccaat gtcaacgatc tcctcatgga
     3061 aaccggcatg tgcgccgact acgccaagat caaagagaac aacgccaagg tcatgaagct
     3121 ggtgcaggga acaaaacatg cccaggtcac caccgacgcc ggcacaaaca tcgaggtcac
     3181 gctcgatccc aactggaagt ggatcaattg cgacggcaac atccagcccg ggcactggtc
     3241 caacctgccc gacggcgagg tctttacctg cgccaagtct gt
//
LOCUS       DATDUN010000324         2312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_100605, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000324 DATDUN010000000
VERSION     DATDUN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100605"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VJC18_04485"
     CDS             <1..1213
                     /locus_tag="VJC18_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44670.1"
                     /translation="GNQAAAWTWTDTTGSFWGRNKLGQKWVEATHAVLANKSDPFFGL
                     RDYFYTAGQRISMLHFALPVAPEYKLLLAGAIFKSLAKPQVPQMPNYHTFVDLFSLLT
                     ESDGALETPLQKTNQARNKQSERPMTQGDVIDTLLGYVLHEAEDPLQYGNIRPVIVTM
                     ALARHYGPHAKQLDAFFVEHGEWLEDLFDFDLSALEFPTGQDVNLKTRHLVATLGRVG
                     IDISSQVNGISPVIEDSAPFKNRQYIVDELDAALYRQLTTRSEQGFSQDDLHRQFEEI
                     EDAFDQQRHEEAKKKVRTLVAVQGNSPHQQEIRAFLETHKWIERELKIDIDDPRWPSL
                     RKHRDRALRIKKEVSISLTEYEPNLRYDATRRVPFWKSAMAFVFGRRSKKQTEAGTET
                     TRFSEGLFVTP"
     gene            1307..2302
                     /gene="galT"
                     /locus_tag="VJC18_04490"
     CDS             1307..2302
                     /gene="galT"
                     /locus_tag="VJC18_04490"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HLD44671.1"
                     /translation="MTDLRKDYVINRWAIIATERSKRPSDLASREGREHFETSAVCPF
                     EMGRETSTPPEVFAYRTEDTPPDTPGWLLRVVPNKFPALAEDQAFEIVDNRLLRSSYG
                     YGVHEVIIEGPNHNLQLSEFDLDDLVLVLRSYRERMLFHYTKNDIRYVQVFKNHGKRG
                     GASLEHSHSQLIALPFVPTLIDMELEGAKKHYESQGQCVFCDFISQEKELGLRIMAER
                     GDMIALCPFASRYPFETWLLPQTHLARFETSPDTILIDTARLLKIVLGKLNEHLNYPP
                     YNFVLHSAPVHCDPSIYHWHIEVIPITTSIAGFERGTECYINTISPENAAAALKK"
BASE COUNT          620 a          544 c          554 g          594 t
ORIGIN      
        1 tggcaatcag gcggcggcct ggacttggac cgatacaaca gggtcgtttt ggggcagaaa
       61 caagttaggg caaaaatggg ttgaggcaac tcatgctgtg cttgccaaca aatctgatcc
      121 tttttttggt ctgagagact atttttacac agcggggcag aggatcagca tgttgcattt
      181 tgccctgcct gttgcccccg aatacaaact gctcttggca ggggctattt tcaagagcct
      241 agcgaaaccg caagtgccgc aaatgcccaa ttatcatacc tttgtggatc tttttagcct
      301 gttgaccgaa agcgatggtg cccttgaaac acccctgcaa aaaaccaatc aggccagaaa
      361 caagcaatcg gaaagaccaa tgactcaggg ggatgtgata gacacgctct tgggttacgt
      421 gttacacgag gcagaggatc ccttgcagta tggaaacatc aggcctgtca ttgtcactat
      481 ggccctggcg cgccactacg gtccgcatgc caaacaactc gatgcctttt ttgttgaaca
      541 tggggagtgg ctcgaagact tgtttgattt tgatttgtcg gcactagaat tccccactgg
      601 gcaagatgtc aatctcaaaa ccagacattt agtcgctacc ttggggcgcg tgggcataga
      661 catctcctcg caagttaatg ggatcagtcc cgtcatcgaa gactccgcac cattcaaaaa
      721 cagacaatac attgtcgacg agctggatgc cgcgttgtat cgtcaactca ccacgcgatc
      781 tgaacagggg ttctcacagg acgatttgca tagacaattt gaggagattg aagatgcttt
      841 cgaccaacaa agacatgagg aggctaagaa aaaagtaagg actctagtgg ctgtccaagg
      901 aaactctcct caccagcagg aaatcagagc ttttttggaa acccataaat ggatcgaaag
      961 agaattaaag attgatattg acgaccccag gtggccgagc cttcgcaaac accgcgatcg
     1021 tgctttgcgc atcaagaagg aagtttctat ctctcttacg gagtatgaac cgaaccttcg
     1081 ttatgacgcg accaggcgag tacctttttg gaaatccgcg atggccttcg tgtttggacg
     1141 acgaagcaaa aagcaaaccg aggctggcac tgagacaacg agattttcag aggggctttt
     1201 cgtgacccct tgaacatatc aacaagactt ctcctgtctt tgtgtgcatt ttcaacttgc
     1261 cagactactg tctttttgga atgaatgctt ttttgagggc ttgttcatga ctgatttaag
     1321 aaaagactat gtcattaaca gatgggcgat cattgcaacc gaacggtcaa agcggccaag
     1381 tgatttggct tcgcgcgagg gtcgtgagca ttttgagaca tctgccgtct gtccctttga
     1441 gatgggcaga gagacctcta cccccccaga agtctttgct tatcgcactg aagacacgcc
     1501 gcctgataca cctggatggt tgctcagggt ggtaccaaat aaatttcctg ctttggcgga
     1561 ggatcaggca tttgagattg ttgataatcg gctcctgcgt tcgtcctatg gttatggggt
     1621 gcacgaagtg atcatagaag gccccaacca caatttgcag ctatcggagt ttgatcttga
     1681 tgacttggtt ctggtgctac gttcttatcg tgaacgaatg ctttttcatt acacaaaaaa
     1741 cgacattcgc tatgtgcagg ttttcaaaaa tcatggcaaa cggggcgggg ccagcctgga
     1801 gcactcccat tcccaattga ttgctctgcc ctttgtacca acactcatcg atatggaact
     1861 ggagggggca aaaaaacatt acgagtcaca aggtcaatgt gtgttttgtg atttcataag
     1921 ccaagaaaag gagttggggt taagaataat ggctgagcga ggagacatga tagctctttg
     1981 cccctttgcc tcccgatatc ccttcgaaac ctggttacta cctcaaaccc acctagccag
     2041 atttgaaacc tctcccgata cgatcttgat agataccgcc aggttgctca agattgttct
     2101 tggcaaactc aacgaacact tgaattatcc tccctataat ttcgtcttgc attctgcgcc
     2161 tgtgcattgt gatccatcca tttaccattg gcacattgaa gtgatcccca tcaccacgag
     2221 cattgctggt tttgaaagag ggactgagtg ttacatcaac acaatttctc cagaaaatgc
     2281 ggcagcagct ctcaaaaaat aaaaaaagtt at
//
LOCUS       DATDUN010000325         2164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_100681, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000325 DATDUN010000000
VERSION     DATDUN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100681"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..959)
                     /locus_tag="VJC18_04495"
     CDS             complement(18..959)
                     /locus_tag="VJC18_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HLD44672.1"
                     /translation="MPTLHKILIQQIALSPLFQTRFQRDDRELASSIQTHGLLIPPVL
                     WQNEAGSFFIIDGAARLACASAIKQEAVACQVMSCAEMNRQQAFVLCLELNRWCRAYN
                     IVEKALLFKQAKNLFAEIPNIPPFFWHAVGIKPHYKIIYQYQNLLKLPDLVLTHAITN
                     DIPINVVITFLKFPPQEQQRIASQLFILPINQNKLGEMLDSLNDISKQEGTQPSEALE
                     QTLRALPGHLTPLEKVQSLRDQLNTRRRPHLIKHQKQFDQSIKKLPLTENTKICPSPF
                     FEDDYIQLTAKLTTQEDVTRLEELLKKIEIDDYLYRP"
     gene            complement(1013..1399)
                     /locus_tag="VJC18_04500"
     CDS             complement(1013..1399)
                     /locus_tag="VJC18_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44673.1"
                     /translation="MINDHQKPGFFMSAQKHKKSFDWQDGETLDDKENYSPMGDVRAT
                     GMPKLKKAVPADPVTRKKERDAKILAEQSLPLQKIRKPQKEGEIIIEESQSGLSGDFE
                     DSQVRKIIDHGKKLRGVDRFGKKKES"
     gene            complement(1430..1705)
                     /locus_tag="VJC18_04505"
     CDS             complement(1430..1705)
                     /locus_tag="VJC18_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLD44674.1"
                     /translation="MTKAELIDRVAKDTEVSKALAEQVINSVTDQVSKCLKKNDKITL
                     TGFGTFMVNKRKARTGRNPQTGAEIKIKATRVPKFKPGKALKDQVSK"
     gene            complement(1915..>2164)
                     /locus_tag="VJC18_04510"
     CDS             complement(1915..>2164)
                     /locus_tag="VJC18_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HLD44675.1"
                     /translation="LPKNIFSYIVKTKPGAGGEIQLTDAIRNIIADTGVNSYVFEGER
                     FDTGEPLGLLLANLVFSLKHPVYKDKIQKTVKMLSAGI"
BASE COUNT          481 a          437 c          558 g          688 t
ORIGIN      
        1 attccgactt gcggaattta gggtctgtac aagtaatcat caatttcaat ttttttgagg
       61 agttcttcaa gacgggtcac gtcttcctgt gttgtgagct tggctgtgag ctggatgtaa
      121 tcatcctcga aaaatggcga agggcaaatt ttggtgttct ccgtgagggg gagtttcttg
      181 atgctttggt cgaactgctt ttgatgtttt atcaggtggg gacgtcgtct tgtgttgagc
      241 tggtcacgaa gggattgcac tttttcaaga ggggtgaggt ggccaggcaa tgccctgagc
      301 gtctgttcga gtgcctcaga tggctgagtc ccctcttgtt tggagatgtc gttgagtgaa
      361 tcaagcatct cacctaactt gttctgattg attggcagga tgaaaagttg actggcgatt
      421 ctttgctgtt cctgcggggg aaacttcaga aaggtgatga ccacattaat tggtatgtcg
      481 ttggtgatgg catgggtcag gacgaggtcg ggtagcttga gcaggttttg atattgataa
      541 atgattttgt aatggggttt gattcccacg gcatgccaaa aaaatggggg aatgttgggg
      601 atttcagcaa aaaggttttt ggcttgtttg aacaacaagg ccttttcgac aatgttgtag
      661 gcacgacacc atcgattgag ttccaaacac aatacgaagg cctgttggcg attcatttcg
      721 gcacaagaca tgacctgaca agcaactgct tcctgcttga ttgccgaggc gcaggccaat
      781 cttgcggcgc catcaatgat aaagaaagag cctgcttcat tttgccagag cacaggcggg
      841 atcaaaaggc catgcgtttg tatcgaagaa gccaactccc tatcgtccct ttgaaatcgt
      901 gtttggaaca agggagacaa ggctatttgc tgaatgagaa ttttatgaag ggtgggcatt
      961 gttgtggtga atgctaaaag gttttatcct aaatcattgc tgttagcctc atttacgatt
     1021 ctttcttttt gccgaagcga tcaaccccgc gcagtttttt gccgtgatca atgatttttc
     1081 tgacctgact gtcctcgaaa tcacccgaaa gaccagactg actttcttcg atgatgattt
     1141 cgccctcttt ttggggtttt cggatttttt ggagaggcaa cgattgttca gcgaggatct
     1201 tggcgtctct ttcttttttt ctggtgacag gatcggcggg aacggccttc ttgagtttgg
     1261 gcattcccgt ggcgcgcaca tcccccattg gcgaatagtt ctctttgtcg tccagcgtct
     1321 cgccatcttg ccaatcaaag gattttttgt gtttttgagc agacataaaa aagcccggtt
     1381 tttgatgatc atttatcata aaccgggctc tgattaaaag aacaatttat tacttgctga
     1441 cttggtcttt cagggctttg ccgggtttaa acttggggac tctggtcgct ttaattttaa
     1501 tttcggcgcc ggtctgtggg ttacggcctg tgcgggcttt tcttttgtta accatgaaag
     1561 taccaaaacc agtcaatgtg attttgtcgt ttttcttcaa gcattttgac acttggtcag
     1621 taacgctgtt gatgacttgt tctgccaatg ccttggaaac ttctgtgtcc ttggctacgc
     1681 gatcgatcaa ttctgctttt gtcataatct ctcctgttga gatgatataa aatcagaggg
     1741 tggcgaacac acaaccatta ggtgtttgcc gccttctgat acgttgccgc gaacaatact
     1801 actcggcaaa gcaatgtcaa ggcgaatcag acgggtttgg aacttttttt ttagcttcgt
     1861 tcactggtca gtcaagcgtt tggtgttgac gttctttgta aagagatgtt gtgcctatat
     1921 tcccgcggag agcattttga ctgttttttg aattttgtct ttgtacacgg gatgcttgag
     1981 tgaaaaaacc aggttggcta acaagaggcc caatggctct ccggtgtcga atcgttcccc
     2041 ctcaaacacg tacgagttca ctcccgtatc ggcaatgatg ttgcgaatgg catcggtgag
     2101 ctggatttct ccccccgcgc ctggttttgt tttgacgatg tagctgaaaa tgtttttggg
     2161 aagc
//
LOCUS       DATDUN010000326         1772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_350418, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000326 DATDUN010000000
VERSION     DATDUN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350418"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VJC18_04515"
     CDS             complement(<1..212)
                     /locus_tag="VJC18_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44676.1"
                     /translation="MNNDSAFVFWLDKFSALPAVTGWSWTPSVRDEFESLFRSRNICA
                     CFDKDTLLLGARSSFDWQNQFEQTRIV"
     gene            complement(219..1667)
                     /locus_tag="VJC18_04520"
     CDS             complement(219..1667)
                     /locus_tag="VJC18_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD44677.1"
                     /translation="MTTHYTSQYLNIYPSHRDSCILFHGITGSIDEVTRALGKLLKKH
                     QRSKTQTDASLPDSVIEFLSSRGHITTLSAHEEWAFFKTYSTELHQKVLEQNKDSLAS
                     FLIIPGYNCNLGCPYCFQKNNAATHSVMTTQTVDTIFERVLPSLCTHLSRPSHAQITL
                     YGGEPFLKPHLPAISRIVEHTQKQQLRISAVTNGTHIDLFKNFFGIAKGLISQVQITL
                     DGDQLTHDRSRKTKSGKGTYDQILKNIHMLIDRGVSVALRVNTTPDNIQSLTALWEHL
                     TREEIVTNPLVTLYLAPIHDFLQSGCQPVFATKHKLTHYLEKTKSPLPSPQNEFTQKF
                     KQVFNASSGIALNTTYFCMQNTGHAFAIDPHQRLYACYEEVGNPDLSVGQFDAQGLVH
                     FNERYDLYKKRNVTTIEACSRCSLALSCGGGCSACARGNQQQDTALFTPACEGFKEMT
                     AKTIRKLFEDKVEGSLCFKKCVPQTAANMPRI"
BASE COUNT          453 a          365 c          474 g          480 t
ORIGIN      
        1 acaattcggg tttgctcaaa ttgattctgc caatcaaaag acgaacgagc gcccaataac
       61 agggtgtctt tatcgaagca ggcacagatg tttctgctgc gaaaaagaga ttcaaattca
      121 tcacgaacgc tgggtgtcca agaccaaccc gttacggcgg gaagagcgga gaatttatcc
      181 agccaaaaaa caaaggcaga atcattgttc ataaacactc atatccgcgg catgtttgcc
      241 gctgtttgtg ggacgcattt tttgaaacac aaagagccct caaccttgtc ttcaaaaagc
      301 ttccgtatgg tttttgccgt catttccttg aatccttcac aggccggggt aaaaagggcc
      361 gtgtcttgtt gctggtttcc tcgggcacag gccgaacaac ctccgccaca ggacaaagcc
      421 agagagcaac gactacaggc ctcaatggtt gtcacatttc gttttttgta aagatcatag
      481 cgttcattaa aatgcacaag tccctgtgca tcaaactgtc ccacagacag atcgggattg
      541 cccacttctt cgtaacaggc atagagtctt tggtgaggat cgatggcaaa ggcgtgacct
      601 gtattttgca tgcagaaata ggtggtgttg agggcaatgc cagaggaggc gttaaaaacc
      661 tgtttgaatt tttgtgtgaa ctcattttgt ggtgacggga gaggtgattt tgttttttcc
      721 agatagtgtg tgagtttgtg ttttgtggca aagactggct gacaacctga ttgcaaaaaa
      781 tcatggatgg gggccagata caacgtgacc aagggattgg tgacaatctc ctcacgtgtc
      841 agatgctccc acaaggctgt taggctctga atgttgtcgg gcgtggtatt gactctcagg
      901 gccacagaga cgccccggtc gatgagcatg tgaatgtttt ttagaatctg atcataggtc
      961 cctttgccag acttggtctt tctgctacga tcgtgtgtga gttgatcgcc atcaagggtg
     1021 atttgcactt gagagatgag gcccttggca atgccaaaaa aatttttgaa gagatcaatg
     1081 tgggtgccat tggtgacggc actgatacgc aactgttgtt tctgtgtatg ttcgacaata
     1141 cgggagatgg cgggcaagtg tggttttaaa aagggctctc ccccatacaa ggttatttgt
     1201 gcatggcttg ggcgtgaaag atgtgtgcat aaagaaggaa gcacgcgttc aaaaatggtg
     1261 tcgactgtct gtgtggtcat cacagaatgt gttgccgcgt tgtttttttg aaaacaatat
     1321 gggcagccca gattacagtt gtaaccgggg atgatcaaaa acgaggctag cgaatccttg
     1381 ttttgttcca ggactttttg atgcagctcc gtggagtatg ttttaaaaaa ggcccactcc
     1441 tcatgagcag acagtgtcgt gatgtggccg cgggaagaga gaaactcgat gacagaatcg
     1501 ggtagggaag catccgtttg tgttttggat cgctggtgtt ttttgaggag ctttcccaaa
     1561 gcccgggtca cttcatcgat ggatccggtg atcccgtgaa acaaaataca actgtccctg
     1621 tgtgagggat agatgtttaa gtattggctc gtgtaatgtg tcgtcataag tcatgggcct
     1681 cgatgaacta attgccgttt aaataagcca aacaccagca attgatgccg ctggcctgca
     1741 agagccggtt gacctggcct tccaggactt gt
//
LOCUS       DATDUN010000327         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_141392, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000327 DATDUN010000000
VERSION     DATDUN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141392"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VJC18_04525"
     CDS             <1..267
                     /locus_tag="VJC18_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44678.1"
                     /translation="KEIITADEAYRDYYFKPNELLHELRGLRILFYNEGFVAGRHVLQ
                     CVAQKPIDKDVAKYHAFDMGSGEGPKKDSSRELLEAMFKKKADS"
     gene            308..526
                     /locus_tag="VJC18_04530"
     CDS             308..526
                     /locus_tag="VJC18_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44679.1"
                     /translation="MPDTASCASFYRSPRTKIPISLFWNRIFNANHWKLGLSLASIGF
                     ILFLVRQFYDGIIMKLCPEGMGFELGVG"
     gene            547..2373
                     /locus_tag="VJC18_04535"
     CDS             547..2373
                     /locus_tag="VJC18_04535"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HLD44680.1"
                     /translation="MNKSIFLTKSDFAVARTCPTKLFYLKNHYPNSLQDDEYLELMAE
                     GGYMVGNLAQLMYPDGIEITLDKGIADAIAQTQEQLKKDNVTLFEPIIVAGNKRIRID
                     ILIKNGDRFELIEVKAKSLDPKKPYLNKNGSIASDWRPYFDDVAFQVIILKEAYPHLR
                     ITPCLLLVDTTKRANVDNLAGLFEIHKVSSYDEGFRSYDVAFTGNAAEVINNGLLIKI
                     DVTRQIADIWNETKTVVQTLEDSLKNGLKKIATPLSVHCNKCEFRDTDVGPCGFKECW
                     GKLADVTPHIFDLFFGSTIGGSKNPLFNSMIAEGKVSLYEIPLDALPEKRGIRQAIQI
                     KHTRENTEWISHELKEKMDACAYPLHFIDFETTRVPLPFHKGMRPYEQIAFQWSCHTI
                     EKQGADPLHSDWINTKDHFPNFEFAKSLMEQAGTGGTVFMWATHENTVLSDIFRQAQE
                     YGYKDDQLIGWLDMMTKKDKEDSYTMVDMCALARNYYFHPDMKGKTSIKSVLPAVWKN
                     YPNIKATPWLTKYYQADQNGRVLSPYETLPPINIGEHHEVIRDGTQAMRAYNEMMIGM
                     HRHDTATKDLWVALLKQYCELDTLAMVVIWNYWQTRFVKNLM"
     gene            complement(2415..2567)
                     /locus_tag="VJC18_04540"
     CDS             complement(2415..2567)
                     /locus_tag="VJC18_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HLD44681.1"
                     /translation="MRKNPLATIQEMATDLEVSTRAIEKQIAKLKESGQITRIGPDKG
                     GHWKVK"
BASE COUNT          729 a          603 c          574 g          676 t
ORIGIN      
        1 aaagagatca tcaccgcgga cgaagcgtat cgcgattatt atttcaaacc caatgagttg
       61 ttgcatgagc tgaggggcct gcggattttg ttttacaacg aggggtttgt tgcaggacgc
      121 catgtgttgc aatgtgtggc gcagaagccc atcgacaaag atgtggccaa gtatcatgcc
      181 ttcgatatgg ggagcggcga gggacccaaa aaagattcct cccgtgagtt gctggaagcg
      241 atgtttaaga aaaaggcaga cagttgaaaa ccagggtcag aatgatagcg ggatttcaac
      301 agaaaagatg ccagataccg catcctgtgc aagtttttat cgatccccac gcacaaaaat
      361 cccaatctca ttgttttgga accgcatctt caacgccaat cactggaaac tcggtctgtc
      421 tttggccagc atcggtttca tcctttttct tgtcagacaa ttttacgacg gcatcatcat
      481 gaaactgtgc ccggagggga tggggtttga gttgggggtg gggtgaaata acgaaaggat
      541 ttttcgatga acaaatccat ttttctcacc aaatccgatt ttgccgtcgc tcgtacctgt
      601 cccacaaaac tcttttacct caaaaatcac tatcccaatt cattgcaaga cgatgaatat
      661 ttggaactca tggcggaggg tggctacatg gtgggcaatc tagcccaact catgtatccc
      721 gacggcattg agatcacgct cgataagggc attgccgatg ccattgctca aacacaggaa
      781 cagcttaaaa aagacaatgt cacattgttt gagccaatca tcgtggctgg caataagcgc
      841 atccgcatcg acatcctcat caaaaacggt gatcgatttg aactcattga ggtcaaagcc
      901 aagtccttgg atcccaaaaa accctatcta aacaaaaacg ggtccattgc atctgactgg
      961 cgaccctact ttgacgacgt ggcctttcag gtcattatac tcaaagaagc ctacccccat
     1021 ttacgcatta ctccctgttt gttattggtc gacacaacca aacgggccaa cgtggataat
     1081 ctggcaggcc tttttgaaat tcacaaagtc tccagttacg acgagggttt cagatcttac
     1141 gatgttgcat ttactggcaa tgcggccgaa gtgataaata atgggttact catcaaaata
     1201 gatgtgacca ggcagattgc tgatatttgg aacgaaacaa aaaccgttgt tcaaacactt
     1261 gaggacagtc ttaaaaatgg actgaaaaaa attgccacac ccctttcggt acattgcaac
     1321 aaatgcgaat tcagagacac tgacgtcggt ccctgcgggt tcaaggaatg ttggggcaag
     1381 cttgccgatg taacgccaca catttttgat cttttctttg gctcaaccat tggcggcagc
     1441 aaaaacccgc tcttcaatag catgatcgcc gaaggcaaag tttcactgta cgagattcct
     1501 ttggatgcat tgccagaaaa acgtggtatc agacaagcca ttcaaatcaa acacacgcgc
     1561 gaaaacacag aatggatttc tcatgaatta aaagaaaaga tggacgcctg tgcttatccc
     1621 cttcacttta ttgattttga aacaacccgc gtcccgcttc catttcacaa aggtatgcga
     1681 ccttatgagc aaattgcctt tcagtggagt tgtcacacca ttgaaaagca gggggctgat
     1741 ccccttcatt cggactggat caacaccaaa gatcactttc ccaattttga gtttgccaaa
     1801 tcattgatgg agcaggctgg aacaggtggc actgttttta tgtgggccac ccacgaaaat
     1861 acggtgctct cagatatttt tagacaggca caagagtacg gttacaaaga cgatcaactg
     1921 attggttggc tggacatgat gaccaagaaa gacaaggaag attcatatac aatggttgat
     1981 atgtgcgcgt tggcgcgaaa ctactatttt catcccgaca tgaagggcaa aacatccatc
     2041 aaatctgttc tgcctgcggt ttggaaaaac tatcccaaca tcaaggccac accttggcta
     2101 accaaatatt atcaagcaga ccaaaatggc cgtgttttaa gcccgtatga gaccctgcca
     2161 cccatcaaca ttggcgaaca ccacgaagtc atccgcgatg gcactcaagc catgcgcgcc
     2221 tataatgaaa tgatgattgg tatgcatcgc cacgacactg caaccaaaga cctttgggtc
     2281 gcattgctca agcagtattg cgagttggat acactggcga tggtggttat ttggaattat
     2341 tggcaaacca ggtttgtcaa aaatctgatg tgattttttt ttgattatct cccagtaacc
     2401 tccttttgcc gaccctattt aactttccaa tggccgcctt tatctgggcc aattcttgtg
     2461 atttggcccg actctttgag ttttgcgatc tgtttttcaa tcgcccgggt tgatacttcc
     2521 aaatcggttg ccatttcttg aatggttgcc aagggattct tgcgcatcaa cgactgtatt
     2581 tt
//
LOCUS       DATDUN010000328         2685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_431118, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000328 DATDUN010000000
VERSION     DATDUN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431118"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="VJC18_04545"
     CDS             complement(<1..1386)
                     /locus_tag="VJC18_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44682.1"
                     /translation="MGILNLNNSLVAPTPDMIADPGELTIGFFQHTVIQVMGGRMPYK
                     FAKESGPGEIDPRVGLFSADDEVGTAVIRVTDTLGDSTTVTIHIERSGVHDINYNGNG
                     SINYSPMEGMFKALELTNDNHFVSVGTSEGDFLVAITDRLGEGDDWMGDDGDITIPVG
                     VGGVAEAKDVILSDGYGLYIAGVAGSGTSNDFAMIKIDGQGTLDDSFGAGGKILTDFV
                     GDDDGANAMIAQGDGKIILGGYAKIGPNMNSALARYTPEGELDTDFGPLENGKVVIPV
                     APGASPDIITSMIRLEDDEFMTVGKALIDGKQRVALMKFNADGTPDVSFGVGGRMITA
                     IGARDVTATKMVYAEGRLYVTGYIRNNTSGKDEIIVLKYKLDGSFDETFSEDGWAVVR
                     SPLPQYSAYGFSILVMTNGVVTIGGAMGNLAAGTKFPLMTRLRSNGRINLDYGTTGFV
                     DFPDAGEGMIFD"
     gene            complement(1335..1670)
                     /locus_tag="VJC18_04550"
     CDS             complement(1335..1670)
                     /locus_tag="VJC18_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44683.1"
                     /translation="MDSDFNKNNICQYNLADDSVRTVVDLRGNIAGFSLSYDAASYLA
                     FQLDAPDASGNPGHTIGLYNLQTEESTVLINGSAPMPSNIVLCPRLVANDRWVSSIST
                     TALWPPRRI"
     gene            complement(1693..1830)
                     /locus_tag="VJC18_04555"
     CDS             complement(1693..1830)
                     /locus_tag="VJC18_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44684.1"
                     /translation="MGFMNILNESTGILESRVIFSVLYDDDSAALWSLPATGGSAAQI
                     C"
     gene            complement(1897..2133)
                     /locus_tag="VJC18_04560"
     CDS             complement(1897..2133)
                     /locus_tag="VJC18_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44685.1"
                     /translation="MLMQIIEPEMNLDCRYSLIADGIDVRLNEEYALLATDADSVVDN
                     IRTSPPVFLKLIPPSEMIPNLSFNVYITGVGSPV"
     gene            complement(2226..>2685)
                     /locus_tag="VJC18_04565"
     CDS             complement(2226..>2685)
                     /locus_tag="VJC18_04565"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44686.1"
                     /translation="TVTKVVYVDDRLYMTGYIRNNISGKDEMIVLKYKLDGTLDATFS
                     SDGWIAQRAQNQTYSAYGNSIAIELNGKIIVGGMMGSMGATPNYPVLMRLKTNGALDI
                     NYGDGGFAEFSGATGSVLDILDVASIGTVYGGFTHDSETGDFTPVIGVMW"
BASE COUNT          704 a          773 c          570 g          638 t
ORIGIN      
        1 atcaaaaatc atgccttcac ccgcatcagg aaaatccaca aaaccagtgg tcccgtaatc
       61 aagattgatc ctgccattcg atcgcaagcg ggtcatgagg gggaatttgg ttcccgccgc
      121 cagattgccc atggccccgc caatggtgac aacaccattg gtcattacca aaatcgaaaa
      181 tccatacgcc gagtattgcg ggaggggaga cctcaccacg gcccaaccat cctcgctgaa
      241 cgtttcgtca aaagaaccat ccagcttata cttgaggaca atgatttcat ccttgccact
      301 ggtgttgttg cgaatgtacc ctgtcacata gagtctgccc tccgcgtaca ccatcttggt
      361 tgccgtcaca tcgcgggcgc caatcgcggt aatcatcctt ccacccacac caaaactcac
      421 atcaggggtt ccatcggcat taaacttcat cagagcaacc ctctgtttgc catctatcag
      481 ggccttccca acagtcataa actcgtcgtc ttccagccga atcatgctgg tgatgatgtc
      541 tggtgacgcc ccgggcgcta caggaataac aaccttccca ttttccagcg gtccaaagtc
      601 tgtgtccagc tccccttcgg gcgtataacg agccagagca gaattcatat tgggaccaat
      661 cttggcgtac cctcccaaaa tgatcttgcc atcgccctgc gcgatcatgg cattggcacc
      721 gtcatcatca cccacaaaat cagtcaggat ctttccacca gcaccgaaac tatcgtccag
      781 agtcccttgc ccatcaattt taatcatggc aaaatcgtta ctggtccccg atcccgccac
      841 tcctgctata tacaatccat acccgtcaga aagaataaca tccttggcct ctgcaacacc
      901 acccacgccc accgggatcg tgatatcgcc atcatcgccc atccaatcat caccctcccc
      961 taggcgatcc gtgatggcaa ccaaaaaatc gccttcagat gttcccaccg acacgaaatg
     1021 gttatcattt gtaagctcta gggccttgaa catgccctcc attggtgaat agtttattga
     1081 gccattgccg ttataattga tatcatgcac acccgaacgc tcaatgtgaa tcgtcacggt
     1141 ggtcgagtca cccaaggtgt cggtcacacg aataacggcc gttcccactt catcgtcagc
     1201 cgaaaacaaa cccacgcggg gatcgatctc gccgggacct gattccttcg cgaacttata
     1261 gggcatgcga ccacccatga cctgaataac cgtatgctgg aagaacccaa tggtgagctc
     1321 accggggtcg gcaatcatat ccggcgtggg ggccacaagg ctgttgttga gattgaggat
     1381 acccacctgt cgttcgccac caatctcggg cataaaacga tgttggaggg catgggggct
     1441 gatccgttga tcaaaaccgt gctttcctcc gtctgcagat tgtataagcc aatcgtgtgc
     1501 cccggattac cggaggcatc gggggcgtcc agttgaaaag ccagatacga cgcggcatca
     1561 tacgataggc tgaatccagc gatattgccg cgcaaatcaa ccactgtgcg cactgaatcg
     1621 tctgccaaat tgtattgaca gatattgttt ttgttaaagt cagagtccat acaaacgaag
     1681 tagacactgt tttcagcaaa tctgggccgc agaaccgcct gttgcaggca gtgaccagag
     1741 ggccgctgaa tcatcgtcgt atagcacaga aaagatcacc cggctctcca ggatacctgt
     1801 cgattcattc agaatgttca tgaagcccat aggtctttcc tgcacctcca tgatgtcgtc
     1861 gacccactcg tcttcataag tgatatattc gctctctcac acaggagagc ccacacccgt
     1921 aatatatacg ttgaacgaaa ggttcggtat catttcggag ggaggaatga gctttaggaa
     1981 aacagggggg ctggttctga tgttatccac aacgctgtcc gcgtctgtgg ccaggagggc
     2041 gtattcctcg ttcaacctga catcaatacc gtccgcaatc aatgaatacc ggcaatccaa
     2101 attcatttcc ggttcaatga tttgcatcaa cacattgact cgaattgatc acagcaaaaa
     2161 caaataaaat ggtgagtatg tatctcctga tgttcatctt atacaacccc gtcctggatt
     2221 tgaaatcacc acatcacacc gatcaccggt gtaaaatcgc ctgtttcaga atcgtgtgta
     2281 aaaccaccat acacagtgcc gattgaggca acatcaagga tatccaaaac actgcctgta
     2341 gctccagaaa actcggcaaa accaccatcg ccataattta tatcaagtgc accgttggtt
     2401 tttaagcgca taagaaccgg atagtttggc gtggccccca tacttcccat catcccacca
     2461 acgataatct tcccattcag ttcaatagcg attgagttcc cataggccga ataggtctgg
     2521 ttctgtgccc tctgcgcaat ccaaccatca ctgctgaacg ttgcgtccaa ggtgccatca
     2581 agcttatatt tgaggacaat catctcatcc ttaccgctta tgttgttgcg aatataacct
     2641 gtcatgtaga gtcgatcatc cacatacacc accttggtta ccgtc
//
LOCUS       DATDUN010000329         3420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_19760, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000329 DATDUN010000000
VERSION     DATDUN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19760"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VJC18_04570"
     CDS             <1..328
                     /locus_tag="VJC18_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HLD44687.1"
                     /translation="GVGIVEAQRGTLTHHYWTDERGIVTKANIIVGTTNNNAAICMSL
                     KKAAQGLIKKGVEVTEGTLNMIEMAFRAYDPCFSCATHRLPGTMPMILTIRDEQGAVI
                     QSVKRD"
     gene            445..717
                     /locus_tag="VJC18_04575"
     CDS             445..717
                     /locus_tag="VJC18_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HLD44688.1"
                     /translation="MYLWDDKKSEANKLKHGISFEEARDHVFEKANVLATDVAYDKGE
                     VRHAVIGKFQGKYFTGIFTVKAQGVRIISVRRSRDEEEKQAKKHGL"
     gene            680..937
                     /locus_tag="VJC18_04580"
     CDS             680..937
                     /locus_tag="VJC18_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44689.1"
                     /translation="MKKKNKPKSTDFDQDFDDGKIAVDFSEGVLTKGLSHLVTLPPLT
                     IPSWLNAELERIAQLQANTKTAVVRQLLVEALLAKKGVSPV"
     gene            951..1532
                     /locus_tag="VJC18_04585"
     CDS             951..1532
                     /locus_tag="VJC18_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HLD44690.1"
                     /translation="MILGLGNDILSDDAVGPKLVWELEKRRDEGRKHEGRGTKTRKHE
                     AQSDVDRGSLIVDRNMQCPIHDSRSTIHDFKTAALGGLELMEMIQGYDQVVIIDAIKT
                     PHGVPGTVYELKPEDFKNALHINNLHDTSFLQALELGKRLGLNLPKQIDIIAVEIVED
                     LLFSNDFSPKVQQKWPEIVTEVEGLVSEKILMI"
     gene            complement(1559..1690)
                     /locus_tag="VJC18_04590"
     CDS             complement(1559..1690)
                     /locus_tag="VJC18_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44691.1"
                     /translation="MLSYFLGDTGSCYFDTTGQSPQSGIPFAGQADWQACEANSVKK"
     gene            1708..2577
                     /gene="metF"
                     /locus_tag="VJC18_04595"
     CDS             1708..2577
                     /gene="metF"
                     /locus_tag="VJC18_04595"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HLD44692.1"
                     /translation="MTRFSDFYQKGFHYSLEVFPPKTGKGVASLLSELKKLEAIKPAY
                     VSVTYGALGSTHALTKDLAIKVYDEVRAHTAFHFTCVGMGKAEIKEYVEVLYKKGIKL
                     ILALRGDTPKGEKVFKPARDGFHHANELVSYLATLHDFSIAVAGYPEKHPEAKNLDED
                     IDNLKRKIDAGAELVITQLFFENHLYYDWLDLVRHKGITVPIVAGIMPILKLSQVKKI
                     TELCGATLPKTLIQKLEACGDDERAMRAIGVEHASIQCADLKQHGVSGIHFYCLNQAE
                     SVLKIVNGSTSLF"
     gene            2660..2977
                     /locus_tag="VJC18_04600"
     CDS             2660..2977
                     /locus_tag="VJC18_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLD44693.1"
                     /translation="MLRHKSPLVAQIRSARLALGITQGMLAKKVGLPQSHLSKIENSQ
                     VNITMDSLIEITRALGLEILLVPRGKITLVKALIESPSTKDNDRLTRPAYELDDEQEK
                     ESP"
     gene            2974..>3420
                     /locus_tag="VJC18_04605"
     CDS             2974..>3420
                     /locus_tag="VJC18_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HLD44694.1"
                     /translation="MNLTRQLNVLLYEEKVGSLHLLPGDKSVFVFEPSYIDQHHRNVL
                     SLSFKSALGDLITETRVTRGRLPPYFSNLLPEGFLREYLAELAGVKPQREFFLLGVLG
                     RDLPGAVRVVWEGREALSFDPEQKESRPAGQKTMRFSLAGVQLKFSA"
BASE COUNT          932 a          746 c          890 g          852 t
ORIGIN      
        1 gggtgtgggg attgtcgaag cccaacgcgg gaccctcacg caccattatt ggaccgatga
       61 gcgcggcatt gtcaccaagg ccaacatcat tgtcggcact accaacaata acgcggcgat
      121 ctgcatgtcg ctcaaaaagg ccgcacaagg tctcatcaaa aaaggcgtcg aagtcactga
      181 gggcacactc aacatgatcg agatggcctt tagggcctac gacccctgtt tttcgtgcgc
      241 cacacaccgg cttcccggta ccatgcccat gatcctcacc atccgcgatg aacagggtgc
      301 agtgattcag agtgtgaaga gagattgaga caatttgcaa ttgttgcaat gggcaatgat
      361 aacaatgcag gtttgtaggg gcgggttcta tacccgccct ttttttggca ttgcaaattc
      421 atataattct aatatataat ctatatgtat ttatgggatg acaagaaaag cgaggcaaac
      481 aaactcaaac acggcattag ttttgaggag gccagggatc atgtgtttga aaaagccaat
      541 gttttggcaa ctgatgttgc ttatgataag ggtgaggtgc ggcatgctgt gataggaaag
      601 tttcagggga aatattttac aggtatcttt acggtgaagg cacagggtgt gcgaatcatt
      661 tcggtcagga ggtcacgcga tgaagaagaa aaacaagcca aaaagcacgg actttgatca
      721 agattttgat gacggaaaaa ttgccgtgga tttcagcgaa ggagtactca cgaaaggact
      781 cagtcatctg gtcacgttgc caccgctaac aatcccttcc tggcttaatg ctgaactcga
      841 gcgcattgcg cagttgcagg ccaatacaaa gacggctgtt gtcaggcaat tattagtcga
      901 ggctttgttg gcaaaaaagg gagtatcacc tgtctgagca gaaacaaatt ttgattttgg
      961 gtcttggcaa cgatatcctt tccgatgacg cggtggggcc gaagttggtt tgggagcttg
     1021 agaaacgaag ggacgaggga cgaaaacacg agggacgagg gacgaaaaca cgaaaacacg
     1081 aagcacagtc agatgtggat cgtggatcgt tgattgtgga tcgaaatatg caatgtccga
     1141 ttcacgattc acgatccacg attcacgatt ttaaaactgc cgcgctgggt gggttggagc
     1201 tcatggagat gattcagggg tatgaccaag ttgtcatcat cgatgccatc aaaacgcccc
     1261 atggtgtgcc cggcacggtc tatgagctca aaccagagga tttcaaaaac gccctgcaca
     1321 tcaataacct tcacgatacc tcgtttttgc aggcgttgga actgggcaag cgtcttgggt
     1381 tgaatcttcc gaaacagatc gacatcattg cggttgaaat tgtggaggat ttgttgttta
     1441 gcaacgactt ctcgccgaag gtgcaacaga aatggccgga gattgtgaca gaagttgagg
     1501 ggttggtgag tgaaaaaatc ctgatgattt gatatttttt gggtcctgct acgggtcgct
     1561 attttttgac actattggct tcgcaagctt gccaatcggc ctgtcccgca aacgggatgc
     1621 cgctttgcgg tgactggccg gtcgtgtcaa aataacagct tcccgtatca cccaaaaaat
     1681 aagaaagcat aatcgaagaa agacctcatg actcgtttca gtgattttta tcaaaaaggt
     1741 tttcattact ctctggaggt ctttcccccc aagacaggga agggtgttgc ctctttgctt
     1801 tctgaactga aaaaactgga ggccatcaaa cccgcttatg tctcggtgac gtatggggcc
     1861 ttgggcagta cgcacgcgct caccaaagat ttggccatca aagtctacga tgaagtgcgg
     1921 gcccacacgg cctttcactt tacctgcgtg gggatgggta aagccgaaat aaaagaatat
     1981 gtggaggttt tgtacaaaaa gggcataaaa ttgattctgg ccctgcgagg ggatacgccc
     2041 aagggagaaa aagttttcaa acctgcgcga gatggctttc atcacgccaa tgaactggtt
     2101 tcttatctgg cgacattgca cgattttagc attgccgtgg cggggtatcc cgaaaaacat
     2161 cccgaagcca aaaatcttga tgaggacatc gataacctca aacgcaagat cgatgcgggt
     2221 gccgagcttg tgatcaccca gttatttttt gaaaaccatt tgtattacga ttggctggat
     2281 ttggtacgtc ataagggcat taccgtgccg atcgttgcgg ggatcatgcc catcctcaaa
     2341 ctcagtcagg tcaaaaaaat cacagagctt tgcggggcca ctctgcccaa aaccctgatt
     2401 caaaaacttg aagcttgcgg tgacgacgaa cgtgccatgc gcgcgatcgg tgtggagcac
     2461 gcttctatac aatgtgctga tctcaaacag catggcgtgt ctggcattca cttctattgc
     2521 ctcaatcagg cagaatcagt gctgaaaata gtcaacgggt caacctcgct cttctgaatc
     2581 acactgccac atctatggcc aggagaggtg tgggatgatt ttgattgata tattttagca
     2641 tataatatat agtatatata tgcttagaca taaatcaccc ttggtggcac aaatcaggag
     2701 cgcgcgcttg gctttgggca tcacacaggg catgctggca aaaaaggttg ggcttccgca
     2761 aagtcatttg tcaaaaatag aaaactcaca agtgaacatc accatggata gcctgatcga
     2821 aatcacaaga gcgctgggtc tggaaattct gcttgttccg cgaggaaaaa tcacattggt
     2881 caaagccctc attgagtcac catcaaccaa agacaatgat cgactcacca gacctgccta
     2941 tgaacttgat gatgaacaag aaaaggaatc accatgaacc tcacgcgaca gctaaacgtt
     3001 ttattgtacg aagaaaaagt gggctcgttg catcttttac cgggtgataa gagcgtcttt
     3061 gtttttgaac cttcctacat tgaccagcat catcggaacg tattgagctt gtcgttcaag
     3121 tctgctttgg gtgacttgat aacagaaacg cgcgtgacgc gagggcggtt accaccctat
     3181 ttttcaaatt tattgcccga aggtttttta agggagtatc tggcagagct cgcgggtgtg
     3241 aagccacagc gtgagttttt tcttttgggc gttttgggga gggatttgcc tggcgccgtt
     3301 cgtgttgtgt gggagggcag ggaggcattg tcctttgatc ccgaacaaaa agaatccaga
     3361 ccggccggac agaaaaccat gcgtttttca ctggcggggg tgcagttaaa gttttcggcc
//
LOCUS       DATDUN010000330         1168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_182038, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000330 DATDUN010000000
VERSION     DATDUN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182038"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VJC18_04610"
     CDS             <1..1074
                     /locus_tag="VJC18_04610"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HLD44695.1"
                     /translation="DRISKEMKKVGFDEVRVDGLGSVIGRIGNGPVKIAFDAHIDTVD
                     VGSRALWSFDPYNAHIKDGKVWGRGVADQKGGMASMVYAGKIIKELGLVKNATIYMVG
                     SVMEEDCDGLCWDYLIRKENLRPDICVLTEPTSLNIYRGHRGRMEIKIKHKGLSCHGS
                     APERGDNAVYKMSKLVLDIEQLNARLKTDDFLGKGTVAVTEFKSVSPSLCAVPDESEI
                     HLDRRLTWGETEESAIQEICKLATASGARVFVPTYEGKGYTGLIHPMKKYFPTWKIPT
                     EHKGVVSAVNGYKKLFDREPQVDKWTFSTNGVAIMGIHNIPCIGFGPGHEPQAHAPDE
                     WTPVDHLSKASAFYAQFVEEMAV"
BASE COUNT          292 a          268 c          339 g          269 t
ORIGIN      
        1 gaccgtattt ccaaagaaat gaaaaaggtc gggttcgatg aggtgcgcgt ggacggtctg
       61 gggtcggtga ttggccgcat tggcaatgga cctgtcaaga tcgcgttcga tgcgcatatc
      121 gatactgtcg atgttggctc ccgtgcgctg tggagttttg atccgtacaa tgcgcacatc
      181 aaggatggca aggtttgggg gcggggcgtg gccgatcaaa agggtggcat ggccagcatg
      241 gtttatgcgg gcaagatcat caaagaattg ggtcttgtca agaacgccac catttacatg
      301 gttggttccg tgatggaaga ggactgtgac ggtttgtgtt gggactacct catccgcaaa
      361 gaaaatttgc ggcccgatat ctgcgtgctc accgaaccca cgagcctcaa catttatcgt
      421 ggtcatcgtg ggcgcatgga aatcaaaatc aaacacaagg ggctttcctg tcatggttcc
      481 gcgccagagc ggggtgacaa cgcggtgtat aaaatgtcaa aacttgtgct cgatattgaa
      541 cagctcaacg ctcgtttgaa gacagacgat tttttgggca agggaactgt ggctgtgacg
      601 gaattcaaat cggtgagtcc ttctttatgc gccgtgcctg atgagtccga aattcatttg
      661 gaccgcagac tcacgtgggg tgagaccgag gaatcggcga ttcaggaaat ttgcaaactg
      721 gccacggcga gcggggcgcg cgtttttgtg cccacgtacg aaggcaaggg ttacacaggt
      781 ctgattcacc ccatgaaaaa atattttccc acctggaaga tccccacaga gcacaagggc
      841 gtggtgtcgg ccgtaaacgg gtacaaaaaa ctttttgatc gcgaaccgca agtcgacaaa
      901 tggacgtttt caaccaacgg cgtggccatc atggggattc acaacattcc ctgcattggt
      961 tttggccccg gtcatgaacc gcaggcccac gcgccggacg agtggacccc cgtggatcat
     1021 ttgtccaagg cctcggcgtt ttacgcgcag tttgtggagg aaatggctgt gtaaactgca
     1081 tttgaggaag tgcaaaacaa ttttctgata aggagtgtgg attaaccaac acatatacag
     1141 ctcaaagctc aaggctcaaa gctcaaag
//
LOCUS       DATDUN010000331         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_598666, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000331 DATDUN010000000
VERSION     DATDUN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598666"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VJC18_04615"
     CDS             complement(<1..797)
                     /locus_tag="VJC18_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44696.1"
                     /translation="MKKALYVFVFFFAFCLTVTSQAVAEEGHQLTTKVPMTLYGFVSA
                     QSMWGDSQVGSFGVNLNMTAPNRVIDETTVAANNDFIGFTLQNTRVGLYLEPYDFGGK
                     SFNIDARIELDFWSTAAAAYAVQTPRIRRAYTSIGTDTWNVLFGQEWDLFAPLNPLTI
                     NVGSVLWNQGNLGSRRPQIRLTTKYAYDQSGIELGASVNSPNNSMLFNDLGNTTGMPM
                     FQGRLGYWREGDSGKYLAYLSGVYGRHDNVVVGGADVNNWGTAFSLDL"
     gene            complement(900..1484)
                     /locus_tag="VJC18_04620"
     CDS             complement(900..1484)
                     /locus_tag="VJC18_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HLD44697.1"
                     /translation="MKKYGITKQDALLLVIDAQERLCSAMDQALLGRMLHQTGLLCLY
                     AKKEGLPVILTEQYSKGLGKTIPSLQKHLDGIPYDYFDKVSFGCAGDGPFMKTLTEKY
                     PDKKIIITGMETHVCVYLTALGLIEKGFSVLVAQDAVISRGREYHQNGLQLMDRLGAV
                     ITNTESLVFQLMQCAGGDTFKAVSGYIKENGLKA"
     gene            1650..>2160
                     /locus_tag="VJC18_04625"
     CDS             1650..>2160
                     /locus_tag="VJC18_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44698.1"
                     /translation="MIVGFFLIRKGQEARLASQNYALSQQARASIEESYYKTKDELDK
                     IKLKIDEYNNKTLEAQKSEAAAREYVTHLQRQIDTLTSKLDTAEKQSTENHDGKVKAL
                     ADMKAAQDILTSERKILEDAKLKFHDAFKGLAATALEGNNQQFLELSKSFFKQQADNI
                     KNDMKQKQIS"
BASE COUNT          601 a          576 c          471 g          512 t
ORIGIN      
        1 agatccaaac taaaagcggt cccccagtta ttgacgtcgg cgcctcccac cacaacattg
       61 tcgtgacgac cgtagacgcc tgaaagataa gccagatatt tgccactatc accttctcgc
      121 caataaccca accgcccttg aaacatcggc atgcctgtgg tgttgcccaa atcgttgaac
      181 agcatgctgt tgttgggact gttgacgctg gcgcccaatt cgataccgct ctgatcgtac
      241 gcgtatttgg tcgtcaaacg gatctgaggt cgacgagaac ccagattacc ttgattccac
      301 aacacgctac ccacgttgat tgtcagagga ttgaggggag cgaacaggtc ccactcttgg
      361 ccaaacaata cgttccaggt atcggtacca atactggtgt aggcgcgtct gatgcgcggc
      421 gtttgcacag cataagcagc ggcggccgta ctccagaaat cgagttcaat ccgcgcatcg
      481 atattaaagc tcttgccacc aaaatcgtag ggctccagat acagacccac gcgtgtattt
      541 tgcagggtga atccaataaa atcgttattg gccgccactg tcgtttcatc gatcacgcgg
      601 ttgggggcgg tcatgttcaa gttcacacca aagctaccaa cttgtgaatc accccacatg
      661 gactgggccg atacgaatcc gtatagcgtc atggggacct tggtggtcaa ctgatgccct
      721 tcttcggcca cagcctgcga ggtcaccgta agacagaaag cgaaaaaaaa gacgaataca
      781 taaagtgctt ttttcatttt gttctccttg aaaataaatt taacaataac aacgtgcgac
      841 tgattattaa ctaaaaacag aaaaggcaat gagaaattac gcaccttgcg ccttatgccc
      901 tacgccttga gcccgttttc cttaatatac cccgacacgg ccttgaacgt atccccgccc
      961 gcacattgca tgagctgaaa taccagactt tcggtattgg tgatgacggc accgaggcga
     1021 tccataagtt gtaaaccgtt ctgatgatat tctctgccac gtgatatgac cgcatcctgc
     1081 gcgaccagca cagagaaccc tttttcgatc aatcccagcg ccgtcaggta gacacataca
     1141 tgcgtttcca tgccggtgat gatgattttt ttatcgggat atttttccgt aagtgttttc
     1201 atgaacgggc catcgcccgc acagccaaaa ctcaccttgt cgaaataatc atagggaatg
     1261 ccatcaagat gcttttgcaa tgagggaatg gttttgccca acccctttga atattgttcg
     1321 gtcagaatga caggcaatcc ttcttttttg gcgtacagac acaggagtcc cgtttggtgc
     1381 aacatgcgcc ccaacaaagc ctgatccatg gcagagcaaa gcctttcttg tgcatcaatc
     1441 acgagcaaca gggcatcttg tttggtgata ccgtactttt tcatatcgga ggcttttatc
     1501 cattagacaa aattccgtca actctgcaaa aaaccaattt cccccttgcg ccggacaatc
     1561 gttcgtagtt agtaatatcg gctatggaat cccaaacttt aacattatta acaggctaca
     1621 tattgtcctg tgtttttttt attttcgcca tgattgttgg tttttttctg atccgcaagg
     1681 gccaggaagc cagactggcc tcccaaaact atgccctctc ccaacaggca cgcgccagca
     1741 tcgaagagtc ctattacaag accaaggatg aactggacaa aatcaagctc aagattgacg
     1801 aatacaacaa caagaccctc gaggcccaaa agagtgaggc cgccgctcgt gagtacgtca
     1861 cgcatttgca aagacagatc gacaccctca ccagcaagtt ggacacggcc gaaaaacaat
     1921 ccaccgaaaa tcacgacggc aaggtcaagg ccctggccga catgaaggcc gctcaggata
     1981 ttttgacttc cgagcgcaaa attctcgaag acgccaaact caaattccat gacgccttca
     2041 aaggcctcgc ggccacagcc ttggagggta acaaccagca atttctggaa ttgtccaaat
     2101 cgttcttcaa acaacaggct gataacatca agaacgacat gaagcaaaaa caaatcagca
//
LOCUS       DATDUN010000332         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_472277, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000332 DATDUN010000000
VERSION     DATDUN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472277"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VJC18_04630"
     CDS             <1..1032
                     /locus_tag="VJC18_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HLD44699.1"
                     /translation="NNTKPEYIMGHSCQAVALIVEAENGALAVPLAARIHEGVVFSNR
                     CKKRLPAKMVSLVRLLGITDPYYFLGDAYYACHHVICGVTDNNGHCLSRVRINAVACH
                     PASTPQKRGRGRPKVYGTKIKLASLFDNLKLMTEAQSPVYGESQVVIRYRCVDLLWKP
                     AGRVIRFVAVDHPVRGRILLMTTDLSLVAIELIRLYGLRFKIEVSFKQELRIIGAYGY
                     HFWMMGMKKIRRCSGNQYLHCESDVYRNAVRRKVAAYHRFIQIGLIAQGLIQYLSSTF
                     PKLVWKSFGSWLCTIRPGVPPSEMVTANALRSTFPEFLMGNNNASTFTKFLLDRIDLN
                     RSEGLRLTG"
     gene            complement(1134..1673)
                     /locus_tag="VJC18_04635"
     CDS             complement(1134..1673)
                     /locus_tag="VJC18_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44700.1"
                     /translation="MAIVSRISRKDPSVTGKPATTGLYEADRVPYLTFSRAHDQQMHY
                     FRALLSDLVSYLTLFCEPAFFLVSGVFPTPQFKLWIPSFVGVGIGFFPAVPGSLLAFF
                     HAGGPQTANLPITHSGIWVKKEATKGTTMLLVRLKWFWEAHASCIGRRLFRVFSTKTN
                     ITSGNQTTESNTFEEKCGE"
     gene            1704..2024
                     /locus_tag="VJC18_04640"
     CDS             1704..2024
                     /locus_tag="VJC18_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44701.1"
                     /translation="MFRPSPKPLIPDRVRKITGSFSWIDHRLITQGWFDVLSRDEILT
                     YLWWTTVGDKNGISFYSDEKTATRLKLSVPQIQTARQGLVEKQLVVYRSGVIQVLDLP
                     RRFL"
BASE COUNT          514 a          483 c          496 g          533 t
ORIGIN      
        1 aacaacacca agccagaata tatcatggga cattcgtgtc aggccgttgc tctcatcgtt
       61 gaagctgaaa acggtgccct tgctgtgcct ctggcagctc gtatccatga aggcgtagtg
      121 ttctccaatc gttgcaaaaa gagattgccg gcaaagatgg tgtcactggt tcggctgttg
      181 ggtattactg acccatacta ttttcttggt gatgcgtact atgcttgcca ccatgtgatt
      241 tgcggggtta ctgacaacaa tggtcattgt ctctctcgcg ttcggataaa tgccgtggcc
      301 tgtcacccgg caagcacgcc ccagaaacgg ggacgtggga gaccaaaggt ctatggcaca
      361 aaaatcaaac ttgcctctct ctttgataat ctcaaactca tgactgaggc acaaagcccc
      421 gtttacggcg aatcgcaggt tgtcattcgt tatcgttgtg tggatctgct ttggaaacca
      481 gcgggcaggg taattcggtt tgttgctgtg gatcaccctg ttcgtggacg cattctatta
      541 atgacgactg atttgtctct ggtcgccata gagctgattc gtctctacgg actacgtttc
      601 aagattgagg tttcatttaa gcaagaactc aggatcatcg gtgcctatgg gtaccatttc
      661 tggatgatgg gcatgaagaa aatccgcagg tgttcaggaa accagtatct ccactgtgaa
      721 agtgatgtct atcgaaacgc agttcgaaga aaagtagctg cctatcaccg cttcattcaa
      781 attgggctaa tcgctcaggg tttaattcag tacctgtcgt caacttttcc gaagttggtt
      841 tggaaatcct ttgggtcttg gttgtgtacg atacgtcccg gcgttccacc ttctgaaatg
      901 gtgaccgcta atgcgctcag atcaacgttt cccgaatttc tcatgggcaa caataatgca
      961 tctactttca cgaaattcct gctcgataga atcgacctca acagatccga ggggttacgc
     1021 ctgactggtt agcctgagaa aaagggagtc ttaagtaaat agtaccgccg atctagaagt
     1081 gtaccaaaaa cgccgcttgg ccggtgtacc tgtaccattg gcgtgtaatt tttttattct
     1141 ccgcatttct cttcaaacgt atttgattct gtcgtttgat tcccgctggt gatgtttgtt
     1201 tttgttgaga agactcgaaa taatctcctg ccgatacagg aggcatgagc ttcccaaaac
     1261 cacttcaatc tcacaagcaa catcgttgtc ccttttgtgg cctctttttt cacccagatc
     1321 ccagagtggg tgatcggcaa gtttgctgtt tgcggcccgc ctgcatgaaa aaacgcgaga
     1381 aggctgccag gaaccgctgg aaaaaagcca atcccgacgc cgacaaagga cggtatccaa
     1441 agcttaaact gtggcgtggg aaaaaccccg gataccaaaa agaacgcagg ctcacaaaaa
     1501 agtgtgagat acgagacgag atcggacagc aaagcccgaa aatagtgcat ttgctgatca
     1561 tgggctctgg aaaacgtgag atacgggaca cgatcggcct cgtaaagccc tgtcgttgcg
     1621 ggtttgccag tcacggatgg gtcttttcgc gagatacgag acacgatagc catacagtgc
     1681 catcatgagg tacagggagc gtcatgttca gaccatcacc aaaacccctg atccctgata
     1741 gggttcgaaa aatcacaggg tccttcagtt ggatcgatca ccgtttgatt actcaaggct
     1801 ggttcgatgt cttgagtcgt gacgaaatcc tcacctacct ttggtggacc acggttgggg
     1861 ataaaaacgg catcagcttt tattccgacg aaaaaaccgc cacccgtctc aagctctcgg
     1921 ttcctcagat acagacagcg agacaaggtc tagtcgaaaa acaactcgtt gtttaccgat
     1981 ctggcgtaat acaagtcttg gatttgcccc ggaggttttt atgatt
//
LOCUS       DATDUN010000333         4983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_390415, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000333 DATDUN010000000
VERSION     DATDUN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390415"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..649
                     /locus_tag="VJC18_04645"
     CDS             239..649
                     /locus_tag="VJC18_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HLD44702.1"
                     /translation="MNTTKGWRVKRVVNDLGKRLKAEQKGPWLEKEGDYLRADPIKIS
                     TALTHLMTLANLTVVSQGATKPSLFEINDYSLHVILYGIDKGELGRIFVGKQGPDNDT
                     CFIKTSASDNIYLAPVNLRPLLMRSLENWSEQSK"
     gene            654..1631
                     /locus_tag="VJC18_04650"
     CDS             654..1631
                     /locus_tag="VJC18_04650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HLD44703.1"
                     /translation="MVKKRTQTVQKKTESKKTKAGLPVRDLVVVPSESLPVVARRQAE
                     LIPIDPLKRYLIEVSRYPIMDAQEEKEVAEHYKKNQDRASAEKLVLANLRLVAKIATE
                     YRSAYYNVLDLIQEGNLGLMRAVQKFDPERGVRFTSYAVWWVRAFILKFILDNFRLVK
                     VGTTQAQKKLFFNLMKEKEKVEKLGFVATSKLISERLDVKETEVIEMQNRLSAREMEL
                     DAPRPSFEGALNMDFIAIPDSSASEQVERQELKEILIKNLDSFTQKLKPKEKEIFQKR
                     LFAEVPKTLQEIADAYGITRERVRQIEERLIQKMREYFKDKGLSVDVHV"
     gene            complement(1674..2219)
                     /locus_tag="VJC18_04655"
     CDS             complement(1674..2219)
                     /locus_tag="VJC18_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44704.1"
                     /translation="MKPYLLHLLMIPALVLLICNPFTYALAYSLSRLLFSSVSPFQSL
                     AVFLVISYGAYLCWFRGRNPRDWVVLGISGVVALFWSFFALPFVVWMAGMILVFSIMR
                     GLIMKKSLSGFGTDLTHTLVGAGAAVFLFPVGLSLSVGVFLIIQFLCDLRSQQPRTVH
                     HPDRFYENLKTVKQILASQSP"
     gene            complement(2299..2838)
                     /locus_tag="VJC18_04660"
     CDS             complement(2299..2838)
                     /locus_tag="VJC18_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HLD44705.1"
                     /translation="MTLLYRIKRLVKANAHGFVDGLEEHQWIVNQAIRDMESELEKQT
                     TFLSEQKQVRIALEQRVQNDDNELSQIEKDIDLALAEKRDDIAKHFIKKSLVGKKRAH
                     TLSAELTSLKAEIGTLEKDLAQKQQAFDDICCQCESAKLSTNQDDVFDAAKVLVEKDL
                     SLEHEVELEFLRRIKKDSK"
     gene            complement(2969..4441)
                     /locus_tag="VJC18_04665"
     CDS             complement(2969..4441)
                     /locus_tag="VJC18_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLD44706.1"
                     /translation="MKFKHLMYSRKLTQWLGLVMLTCLIAYGAQAKIWPDPKITNCPV
                     GANCITPTTVTPPVATWQKSGPVQAKAQLSQYKVLQGSDGMIYLKVDLEALDNDLINN
                     TSRKPTDFVVVLDRSGSMSEKNKIEYARKAVTALLGQLKPEDRFSLVSFDTIVENPIP
                     LTKVSSANLETLTTLDNSIAPRDSTNLEGGLTRGMDILKELGKTSGNARRMILVSDGL
                     ANVGLSDTPALSRIADGAVPGEFVISTIGVGLDFNENLLSALADHGTGSYYYLENPSG
                     IDKVLLAEFFNASSILAKNLKLTFDLAGGFDIVDASGYPVTVDGRVKIVQPGHLYDQQ
                     KRTMYVSIKAPTNTIYAEALGQITLSYEINNTTHQLKLIDSNVMISCLPQEKRDEVLA
                     SIDKETFSKAWTQNNVGYFMKDSAKKINKGEYNQAKAGLKEFKDRLVNAYSAAPTPAM
                     KQQIDDLDKMETQMEAGIASGATKSLSKDMQYKGIEKQRN"
     gene            4649..>4983
                     /locus_tag="VJC18_04670"
     CDS             4649..>4983
                     /locus_tag="VJC18_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD44707.1"
                     /translation="MKPKILIVEDEEAILKGLTDVFLYHGYEVASAMNGREGLNLALT
                     GTYHLIILDIMLPEMDGLTICNHIRDKDRSQPIIMLTAKGDEDDVVAGLKQGADDYVT
                     KPFSIKELLA"
BASE COUNT         1277 a         1159 c         1279 g         1268 t
ORIGIN      
        1 tcgtacaccc ccacaaatcc tttgaccggc gtttgtttcg aacttaaaca cgaacacgat
       61 gttgagaaga ttgtgtgtca ttattctggg tctcttgttc tgtcttttct ttggctggta
      121 cattgtgtcc gcagataggg cccccaacaa aaccactcaa acaggccccc tttttgggtt
      181 ctcacaggat gcgattgcgg cctttcaggt caatcaattc acccaaggac tgtattttat
      241 gaatacgaca aagggttggc gggtgaaaag ggttgtgaac gatttgggaa agaggcttaa
      301 ggcggaacaa aagggaccat ggctcgaaaa agagggggat tatctgagag cagaccccat
      361 caaaatcagc accgccctga cacatctcat gaccctggca aacctgacag tggtttcgca
      421 aggggcaaca aaaccctcgt tatttgagat caatgattat tcgcttcacg tgatccttta
      481 tggtattgac aaaggggaat taggacgtat ctttgttgga aaacagggcc cagacaatga
      541 cacctgtttc attaaaacgt cggcctcaga caacatctac ctggcccctg tgaacctgag
      601 gccattgctc atgcgctcgt tggaaaactg gagtgagcag tccaagtaag ggtatggtaa
      661 aaaagagaac acaaactgtt caaaagaaaa cggaatcaaa gaaaaccaaa gccggcctcc
      721 ctgtgcgtga tttggtggtt gtgccgtcgg aatcattgcc ggttgtggca cgtcggcagg
      781 cggagctgat cccgatagac cccctcaaga ggtatttgat tgaggtcagt cggtatccca
      841 ttatggacgc gcaggaagaa aaagaagtcg cggagcatta caaaaaaaac caggatcgcg
      901 cgtcagccga aaaactggtt ctggcgaacc tgagattggt ggccaagatc gccaccgagt
      961 accgcagtgc ctattacaac gtgctcgacc tgattcagga aggcaatctg gggctcatgc
     1021 gggctgtgca aaaattcgat cccgaacgcg gtgttcgttt cacctcgtac gccgtttggt
     1081 gggtgcgggc cttcatctta aaattcattc ttgataattt tcgactggtc aaggtgggga
     1141 ccacgcaggc gcagaaaaaa ttgtttttta atctgatgaa ggaaaaggaa aaggtcgaga
     1201 agttgggttt tgtggccacc tccaaactca tttcggaacg cctggatgtg aaggagaccg
     1261 aagtgatcga gatgcaaaat cggctttccg cgcgcgaaat ggaacttgat gcgccacgcc
     1321 ccagttttga aggggcactc aacatggatt ttatcgcgat cccggattcg tccgcgtctg
     1381 agcaggtgga gcggcaggaa ctcaaggaaa tcctcatcaa aaaccttgac tcgttcaccc
     1441 aaaaactcaa accaaaggaa aaagagattt ttcagaagcg actctttgca gaggttccca
     1501 aaaccttgca ggagattgcc gacgcgtacg ggatcacccg ggaacgcgtg cgccagatcg
     1561 aagaacgatt gattcaaaag atgcgggaat attttaaaga caagggcctc agtgtggatg
     1621 ttcacgttta gccagttgtc caagcctttt ctgtggggcc gttacagggt ttgtcacggt
     1681 gactgtgagg ccagaatttg ctttaccgtt ttcagattct cataaaaacg atctgggtgg
     1741 tgcacggttc ggggctgttg acttctcaag tcacacaaaa actgaatgat cagaaacaca
     1801 ccgacgctca acgatagtcc cacagggaaa agaaatacag ccgcaccagc accgaccagg
     1861 gtgtgtgtca aatccgtgcc aaagcctgac aaactctttt tcatgatcag tccgcgcatg
     1921 atcgaaaaga ccaaaatcat cccggccatc caaacgacaa agggcagagc aaagaagctc
     1981 caaaagagag ccacaacacc gctgatgccc aaaacgaccc agtcgcgagg gtttctgccg
     2041 cgaaaccagc agagataagc tccgtaagaa atgacaagaa aaacagccag agattgaaaa
     2101 ggcgaaacag aggaaaacag gagtcgcgac aaggaatacg ccaaagcgta cgtgaagggg
     2161 ttgcagatca gcaggacgag ggcggggatc atcagtaaat gcaaaagata gggtttcatg
     2221 ggagctccta ttcgtgggtc gtggatcgtg attcgcctgt gccctttagg gtatgaatcg
     2281 caaaacacgc cccacaattt atttgctgtc cttcttgatt cgtctgagaa attccagttc
     2341 cacttcgtgt tccaggctca gatccttttc cacaagcact tttgccgcat cgaagacatc
     2401 atcctgattg gttgacaact tggccgattc acattgacag caaatgtcat cgaaggcctg
     2461 ctgtttttgg gccaaatcct tttcgagcgt gccaatctcg gctttcaggg aggtcagttc
     2521 ggcggacaag gtatgtgccc tctttttgcc gaccaggctt tttttgatga agtgtttggc
     2581 aatgtcatcg cgtttttctg ccagggccag gtcgatgtcc ttttcgattt gtgataattc
     2641 attgtcatcg ttttggacac gttgctccag agcaatgcgc acttgttttt gttcggaaag
     2701 gaatgttgtt tgtttttcaa gctcggattc catatcacga atggcctgat tgacgatcca
     2761 ttggtgttct tcgaggccgt ccacaaatcc gtgggcattg gctttcacaa gtcttttgat
     2821 gcggtataat agtgtcatga tagtcctttc gtgattcgtg gatcgtaaat cgtggatcgt
     2881 gttattcgat tcataccctc aaaggcataa acgatccata ccctaaaggg cacaagcgat
     2941 ccacgagcca caatccacga tgcacgtttt agtttctctg cttctctatc cccttgtatt
     3001 gcatgtcttt gctcaggctt tttgtggcgc cactggcaat gccagcctcc atctgcgttt
     3061 ccatcttatc gagatcatcg atttgttgtt tcatggcggg agttggtgcg gcactgtacg
     3121 cgttgaccag acggtctttg aactccttga gtccggcctt ggcctgattg tattcaccct
     3181 tattgatttt tttagcactg tccttcatga aataaccgac gttgttttgt gtccaggcct
     3241 tggaaaaagt ttctttgtcg atggaggcca gaacctcatc tcttttttcc tgtggcagac
     3301 acgagatcat cacgtttgaa tctatgagct tgagttggtg tgttgtgttg ttgatttcat
     3361 aagacagggt gatttgaccc agggcttcgg cgtagattgt gtttgtgggg gccttgatgc
     3421 tgacgtacat ggtccgcttt tgttgatcat acaaatgacc gggttgcacg attttgaccc
     3481 taccatccac agtgacggga tacccggagg catcgacgat atcgaacccg ccagccagat
     3541 cgaaggtgag tttgaggttc ttggccaaaa tggaactggc gttgaaaaat tcagcaagca
     3601 agaccttgtc gataccagag ggattttcca gataatagta agagccagtg ccgtgatcgg
     3661 ccagggcaga caacaggttc tcgttgaaat ccaatcccac accgattgtt gagatcacga
     3721 attctcccgg gacggctccg tctgcgatgc ggctcaaggc aggggtgtcc gaaaggccaa
     3781 cattggccaa accatcagaa accagaatca tccggcgggc attgcccgaa gttttaccaa
     3841 gttctttcaa aatgtccatg ccgcgcgtca gtcccccttc cagatttgtt gaatctctgg
     3901 gtgcaatgct gttgtccaga gtggtgaggg tttccagatt ggccgaagaa accttggtga
     3961 gggggatggg gttttccacg atggtgtcaa aagaaacgag cgagaaccga tcttctggtt
     4021 ttaattgccc cagaagcgcg gtgacggcct tgcgggcata ctcaattttg tttttttccg
     4081 acatgctgcc actgcggtcc agcaccacaa caaaatccgt gggtttacgt gaggtgttat
     4141 tgatgaggtc attatccaga gcttccagat ccactttgag atagatcatg ccatcggatc
     4201 cctgcaagac cttgtattgg gagagttgag cctttgcctg cacggggcca ctcttttgcc
     4261 aggtggccac aggcggagtc actgtggttg gtgtaatgca gttcgcgccg acaggacaat
     4321 ttgtgatctt tgggtcgggc cagatttttg cctgtgcccc ataggcgatc aggcaggtga
     4381 gcatgaccaa ccccagccat tgtgtcagtt ttcttgaata catgagatgt ttgaatttca
     4441 tacgattctc cttttttgcc tcgccggagt ctcgacgaag gcgggttttc tatttcacac
     4501 acatcataac gcatcagggg gtgaaatgtg agacaaagga ttgtaaaagg actgtgtttt
     4561 cgttttacaa atgttttaca atttcaccga aaggaattag gtagaatata agaaacgact
     4621 gacattccat ggtgtcgagg gaaatgacat gaaaccaaaa attctcattg tagaagacga
     4681 ggaggcgatc ctcaaaggac tgaccgatgt gtttttgtac cacggttacg aagtcgcatc
     4741 ggcgatgaac ggcagagagg gacttaacct ggccctgacg ggcacgtatc acctcatcat
     4801 tctggacatc atgctcccgg aaatggatgg gttgacgatc tgcaatcaca ttcgtgacaa
     4861 agaccgctca caacccatca tcatgctcac agccaagggc gatgaagacg acgtcgttgc
     4921 gggattgaag cagggtgctg atgattacgt caccaaacca ttttccatta aagaactttt
     4981 ggc
//
LOCUS       DATDUN010000334         2492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_141680, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000334 DATDUN010000000
VERSION     DATDUN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141680"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1926
                     /locus_tag="VJC18_04675"
     CDS             <1..1926
                     /locus_tag="VJC18_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44708.1"
                     /translation="KPRAGSGALTAGLAGAYVVTEVAHEAFDSPEWLRKTTDAGLLGT
                     AVALTPPSTLLPSLAPGLGGAMMVGEASRTLTDATEWSPLVDTTVETTASIGGFYAGV
                     SVTSAGTAIVCTELGIEVASLSGMLVGTGVGVGVGLALLGGYALFRHYNKQNAFEAWR
                     EDFLGKIPEFFESPNNPNLIHEIRTKFFSIESAIANMGGSIDTADELAQLQVIRDQLQ
                     AEKSQPEEKQPATLALAFMGDYLYERYFKKMKVGGEVSAQKLTAINLLRDIRFPGFSG
                     MCARVQSILTTGSETRANRDQTLSQLRTLTEQASGFEKLMFQKCGITLVEDYINPWLK
                     KAYEHETTKQEGEQQLTQTQKNDQAIDFITMHASLCELNDYIRDPFAFEEQARQEQQF
                     YQQTHTRIESRSDPQSIETFRQNVAEVLARNNPDDVRLFIHSLGLPYSQEQMLIDLCD
                     FLKPDELREAVVTASVDQGQHIRTTQLSQQPRIEKPDEAKLQAFDDYAEATYNTLRAQ
                     HRENPWGPVGEHFTDTFRVNFLHELDLKFLEIDRTYSSQQPDSAATWIKLHMLKMWMS
                     DVVLYLEEQGFEEADLSLDPEDLEKWSRTYGQLETIEKLIDSFEQTGDLELLGSPRSQ
                     KDDCIFKLMLDVGRAMQ"
     gene            2031..2104
                     /locus_tag="VJC18_04680"
     tRNA            2031..2104
                     /locus_tag="VJC18_04680"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2065..2067,aa:Pro,seq:tgg)
BASE COUNT          634 a          690 c          604 g          564 t
ORIGIN      
        1 aaaccacgag cgggctctgg tgcgctcaca gcaggactcg cgggtgcgta tgttgtcacc
       61 gaggtggcac acgaggcctt cgattccccc gaatggttgc gcaaaaccac cgacgcgggt
      121 ctgctgggca ccgctgtcgc actcacccca cccagcacac ttctccccag cctggccccg
      181 ggtctgggcg gcgccatgat ggttggcgag gcttccagaa ccctcacaga cgccacagaa
      241 tggtctcctc tggtggacac caccgttgaa accaccgcaa gcattggtgg tttttacgcg
      301 ggcgtctctg tcacctcggc gggcacagcg attgtctgta ccgagctggg tattgaggta
      361 gccagtttga gcggcatgct cgttggcacg ggtgtgggcg ttggcgtagg actggccttg
      421 cttggcggtt acgccctgtt cagacattac aacaaacaaa atgcctttga ggcgtggaga
      481 gaggacttct tgggcaagat ccccgaattt tttgagtccc ccaacaatcc caatttgatc
      541 cacgagattc gcacaaaatt tttctccatt gaatctgcca tcgccaacat gggtgggagc
      601 attgataccg cggacgagtt ggcacaattg caagtgatcc gtgatcagct acaggccgaa
      661 aaatcacagc cggaggagaa acaaccggca accctcgcac tcgccttcat gggcgattat
      721 ctttacgagc gttattttaa aaaaatgaag gttggcggtg aggtgagcgc gcaaaaactg
      781 acagcgatca atctgctcag ggacatccgt tttccgggat tctccggcat gtgcgcacgt
      841 gtgcaaagta tactgaccac aggttcagag acacgcgcaa atcgtgatca aaccctgtca
      901 cagctacgga ccttgactga gcaggccagc ggttttgaaa aactcatgtt tcaaaaatgt
      961 ggcataactc ttgtagagga ttacatcaat ccgtggctca aaaaagcgta tgaacacgaa
     1021 acgaccaaac aagagggcga acaacaactg acgcagaccc aaaaaaatga tcaggccatc
     1081 gatttcatca ccatgcacgc ctcactgtgt gaactcaatg attacatccg agaccccttt
     1141 gcgttcgagg aacaggcccg gcaggaacaa caattctacc aacaaacaca cacacgcatt
     1201 gagagtcgtt cagacccgca aagcattgag accttcagac agaacgtcgc cgaggtactt
     1261 gcccgcaaca accccgatga tgtgaggttg tttattcaca gcctgggcct cccctattct
     1321 caggaacaaa tgctcattga tctgtgtgat tttttgaagc cggatgaact gcgagaagcc
     1381 gtggtgacgg cctcagtcga ccagggccag cacatcagaa ccacccagct ttcgcaacaa
     1441 ccccgcatcg aaaaaccgga cgaagcaaaa ctgcaggcgt ttgatgacta tgccgaagcg
     1501 acttacaata ccttgcgggc ccagcaccgc gaaaatccct ggggtcccgt tggggaacat
     1561 tttacagata cgttccgtgt aaattttttg cacgaactgg atctcaagtt tttggaaatt
     1621 gatcgcacgt actcgagtca gcaacctgat tctgcggcca cctggatcaa attgcacatg
     1681 ctcaagatgt ggatgagtga tgtggtactc taccttgagg aacaggggtt tgaagaagct
     1741 gatttgagtc ttgatcccga ggatctggaa aaatggtccc gcacctacgg ccaattggaa
     1801 accatcgaga aactgatcga ttcttttgaa caaacaggcg acctcgaatt gttgggctcc
     1861 ccccgttcgc aaaaggatga ttgcatcttt aaactcatgt tggacgttgg ccgcgccatg
     1921 cagtaaggtg ccagtcaacc atttctacca caatcaacca tttcaaccat ttctactctt
     1981 tacagaggag tacaaaaatt gtagtggtcc cctcacaagt gttttttgtt cggggagtag
     2041 ctcagcccgg aagagtgcct gctttgggag caggacgtcg caggttcaaa tcctgtctcc
     2101 ccgacattga aaaaagccca ttcacgaagt gattgggctt tttgttttct atcgattgca
     2161 ttttctcctt tacgtgttat tacgtccgcc atcatgacga taataaaaca tctgcatcct
     2221 gtcctttcgt ccatcccacc tacgctcttc gagcttccac ctgcgctgaa gcttcggtgg
     2281 acaagtctgt gggcatgcgt tctttcactc ctcgtgaccc tcctgccagg ctgtactcca
     2341 ggccaggccc aggcacgaga acaaggattg aacaaccaac accagctcaa tcaaactgtt
     2401 accatgaccc caacccctcc gcctctcacg tttgccaccg cggatttcat gcgcgagctc
     2461 aacgcctaca tcaaaacagt ccaaaaaacc tg
//
LOCUS       DATDUN010000335         1444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_431684, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000335 DATDUN010000000
VERSION     DATDUN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431684"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VJC18_04685"
     CDS             complement(<1..1290)
                     /locus_tag="VJC18_04685"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HLD44709.1"
                     /translation="MLTRQAAHALLDQLAGQKCDFADVYDEQVTETTIRVQDKQLKTC
                     QTGVTQGISLRVIDGLKTRHAYTNSTKKADWHQLVSELNLSDHVRRGTRHIRIKQEKS
                     HLPYSVATPRDKTDHQDIIALCHELSDNTLAHDQRIQQVVLSYHDRERLVRICNTDGK
                     DIREILKILTLSISITAKDNHDCFTSSEIISRLGGQDIVCDSDLKPIVHDTVERALRH
                     FGAKSPKGGVLPVVLSGSAGGTIIHEAVGHGLEGDLVIEGMSLFRGKIGQLIAHPKVT
                     IVDDATIPNLRGSFSFDDEGTPSQSNVLIENGVLKTYLCDRLSASRLGIPPTGNGRRE
                     SFEHPPLTRMTNLFLSSGTDKTDNIIASVADGLFVTKMGGGQVNPVSGDFMFRVQEAF
                     EIKHGKIGTPLKNVTLTGNGPEMMKHITMMSGDPSFVP"
BASE COUNT          306 a          332 c          420 g          386 t
ORIGIN      
        1 gggaacaaaa ctggggtcgc ctgacatcat ggtgatgtgt ttcatcatct cgggaccgtt
       61 gcccgtcagc gtgacatttt tgagaggggt tccgattttg ccgtgcttga tctcaaaggc
      121 ctcctgcacg cgaaacatga aatcaccgct gaccgggttg acctgccccc cacccatttt
      181 ggtgacaaac aacccgtctg ccacagaggc gatgatgttg tcggttttgt cggtgcccga
      241 ggagagaaat aaattggtca tccgcgtgag aggggggtgt tcaaaggatt cgcgcctgcc
      301 gttgccagtg ggtggtatcc ccagtcgcga ggcagacaag cggtcacaga ggtaggtttt
      361 caacacgccg ttttcgatca agacgttgct ttgcgatggc gtgccttcgt cgtcaaaact
      421 aaaagacccc ctgaggtttg ggatggtggc atcatccaca atggtgactt tggggtgggc
      481 gatgagttga ccaattttgc cgcgaaacag cgacatcccc tcaatcacca gatcaccttc
      541 gagcccgtga ccaacggctt cgtggatgat tgtgccaccc gcggatccag acaacacaac
      601 cggcagaaca ccccctttgg gtgatttggc tccgaagtgc cgcagggccc tctcgactgt
      661 gtcgtgcaca atcggtttta gatcggagtc acagacgata tcttgaccgc ccagacgcga
      721 aatgatttcg gaggaggtaa aacaatcatg gttatctttt gcggtaatgc tgatcgataa
      781 ggtcaatatt tttaaaattt cgcgaatatc tttgccgtca gtgttgcaga tgcgtaccag
      841 tctttccctg tcatgatagg agagaaccac ctgctggatc ctctggtcgt gtgccagtgt
      901 gttgtcactc agttcgtggc agagtgcgat gatgtcctga tggtcagtct tatcacgtgg
      961 cgttgcaaca gaatacggga gatgcgattt ttcctgttta atacggatgt gtctggtgcc
     1021 acgcctgacg tgatctgata gatttagttc agagaccagt tggtgccaat cggctttctt
     1081 ggtgctgttg gtgtaggcgt gccgggtttt cagtccgtca atgactctca ggctaatgcc
     1141 ttgtgtgacc ccagtctggc aggtcttgag ttgtttgtcc tgcacacgga ttgtggtttc
     1201 ggtgacctgt tcgtcataca cgtcggcaaa gtcacatttt tgcccggcga gttggtcgag
     1261 cagggcatgg gcagcttggc gggtgagcat cgctccttat ttcgcagaat gtcgatatta
     1321 tcaagtttca aagggggtaa tggtagggga ttgttttttt aatagggacg ggttatggcg
     1381 tgggggaaga ttgcgaaagg aaccttatgc aagaatacat tgggatcatt ggtgggagcg
     1441 gctt
//
LOCUS       DATDUN010000336         4124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_515566, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000336 DATDUN010000000
VERSION     DATDUN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515566"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VJC18_04690"
     CDS             <1..254
                     /locus_tag="VJC18_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44710.1"
                     /translation="ITEKGRPVSSEHNIFVKGGGCRCWLIELIKKMVIHVRENRIQRL
                     QDKLEKIGDSDPTDEAMNKKAVYEKRIRRITEHMVSATE"
     gene            470..1882
                     /locus_tag="VJC18_04695"
     CDS             470..1882
                     /locus_tag="VJC18_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLD44711.1"
                     /translation="MSYNILCVDDDPRFLIGIRAALMGDYAVFTAENFDVAANLLNQN
                     SIDVVLLDLELQTITGLEALKLIKARYPDVEVLMLSGYKNPKDIVEAMRAGASDYLTK
                     DTCPEEIIVYINKLLKNKEIKDRYSALIESTKPEQVKLPFMGKAKAFEKILSQARRLK
                     GINAHVLIEGESGTGKELLARYIHDIESDPKRPFIAVNCAAIPENVLESDLFGHEKGS
                     FTGATERKIGKFELADNGDILLDEINSLKIELQGKILRALEEKEFYRVGSIKPISVNF
                     RVIACANVCLSKLVAEGKFREDLYHRLHVIALCIPPLRERRDDILGFINHFTNQFSQY
                     YQRMNKKTFTDRALQFLYQYAWPGNVRELKNLIQSLTIMCERDVVDVCDLPHSMLTDA
                     KIKKEGVATQEDDKDSHHLFSQFDKTLNEFRLQAERQYVKCVIKANQGNKTRAAKVLG
                     VSRPTLHAKIRLDEVTRIGK"
     gene            1893..>4124
                     /locus_tag="VJC18_04700"
     CDS             1893..>4124
                     /locus_tag="VJC18_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44712.1"
                     /translation="MTVFKRSNTIRNSKHKVQGICDVATLAVKQSLTKTLLVCLGILI
                     VGVWLLIQLVIVPFMAELKDLRAQTSHSLFERNREIMRSTVELGASNIQQLFAATEVA
                     VNTMITDLHSPKVKMAKSPFLPTAQEYLTADAIKKSMSPHPLYPDRLVSLEYPRVYYP
                     SDEMSDARALEEKNLQGYWPSFEKTVSSLHSLTGEFKWLYVASETGMLLVYPGSDNVP
                     KGYDPRVRPWYVGAMGRQAGYWTPPYYTAGGNEIVITFSQPLGQHAFMKNAVVAADLV
                     LTDIINKVLFTPLCDECKIYLVNERDEIIAQREPATLKASWTEPLPVKTLGEVLASRA
                     DVVGEGNDGQHNIFSVSIDRLHWRLVGVIYNHSLGNDGGQIIARISSFSQKNKTALMI
                     IMGVVLAIVLILMGTLYLIVRRTMRTKMEPSLDQFKRLAQNLDAVHLDNVLSLMAGDA
                     TNVTESLTHEHKIVVEALQRLGDKLLNAEKQIETKSKLAAVGETAGQVAHDIRSPLTA
                     LSVIAAELGELAEEKRLLIRSAVQRIEDIANDLSSKRVQRPKTNDQSQKQGEIVIPAK
                     AGIQNITPTVDPSLRWDDKTNPTLKGTSDTLSLPPLATNSHLLSAVVESLVSEKRTQY
                     RNRSGVTIEANLGSESYGLFANIQLDQFKRVLSNIVNNAVEAMEGGGSLVVAMRAVEP
                     QSHRAYDSEFTEAPTPHGSMAQDIQITIIDTGKGIPAEILPRLFRCGESFGKIDGAGL
                     GLYL"
BASE COUNT         1130 a          945 c         1094 g          955 t
ORIGIN      
        1 aaataaccga aaaaggtcgt ccagttagta gtgaacacaa cattttcgtc aaaggcggag
       61 gctgcaggtg ttggttaatc gagctgataa aaaagatggt catacatgtg cgtgagaata
      121 gaatccaaag attgcaggac aagctcgaaa aaattgggga ttctgatcct acagacgaag
      181 ccatgaacaa gaaggcagtt tatgagaaaa ggattcgtcg cataacagaa catatggtaa
      241 gtgctactga gtaattattg ggaagattga actggctaca tttttttgcg accccccccc
      301 ttttttcacc gttcaacacc ccttacactg taacgatacc tttacagtta actgtccaac
      361 atcttgcaca gaatttatgc ggcacaaaca atcccttgta agcattgcat aaatatttat
      421 gagaccgtgg tacgacgatt gcatagtaca aaggcaaggg gtgtgtacta tgtcatataa
      481 tatactttgt gtggacgatg atccacgttt tttgattggt atccgggccg ccctcatggg
      541 cgactacgcg gtcttcacgg ccgaaaattt tgatgtggct gcaaatctgc tcaatcagaa
      601 tagcatcgat gtggttctgc ttgatctgga gttgcaaaca atcacggggc ttgaggcgct
      661 gaagcttatc aaggcacgat atcccgatgt tgaagtgttg atgttgtcgg gttacaaaaa
      721 tccaaaggac atcgtggagg ccatgcgggc tggtgcctcc gattatttga ccaaagacac
      781 ctgccccgaa gaaatcattg tctacatcaa taaactgctc aagaacaaag aaatcaagga
      841 tcgttacagc gccctcatcg aaagcacaaa acccgaacag gtaaagctcc ccttcatggg
      901 caaagcgaag gccttcgaaa aaattctgag tcaggccaga cggctcaagg gcatcaacgc
      961 ccacgtgctc atcgaaggag agagcggcac aggcaaggaa ctgctggctc ggtacattca
     1021 cgacattgag tccgatccga aacgcccctt catcgccgtg aactgcgccg ccattccgga
     1081 aaatgtattg gaatcagatt tgtttggaca cgaaaagggt tccttcacgg gcgccacaga
     1141 aagaaagatt ggcaagtttg aattggccga taacggcgat atccttttgg acgaaatcaa
     1201 ttccctcaaa atagaactgc aggggaaaat cctgcgcgct ctggaggaaa aagagtttta
     1261 ccgcgtgggg agcatcaaac cgatctccgt caactttagg gtaatcgcgt gtgccaatgt
     1321 gtgtttgtcc aaattggtgg ccgaggggaa attcagggaa gacctgtatc atcgcctcca
     1381 tgtcattgcc ttgtgcatcc ccccgctgag ggaacggcgc gacgacattt tggggttcat
     1441 caatcatttc acaaatcaat tcagtcaata ttaccagagg atgaacaaaa agacattcac
     1501 ggatcgggcg ttgcaatttc tttatcagta tgcgtggccg ggcaatgtgc gtgagctgaa
     1561 aaatctgatt cagagcctca cgatcatgtg cgaaagggat gtggttgatg tctgtgatct
     1621 gccgcatagc atgttgactg atgcaaaaat caaaaaggaa ggcgtggcga cacaggagga
     1681 tgataaagac tcccatcatc ttttttcgca gtttgacaaa acgctcaacg agttcagact
     1741 acaggcggaa cggcagtatg ttaaatgcgt gatcaaggcg aatcagggca acaagaccag
     1801 ggcggcgaag gttctcggtg tgtcgcggcc caccctgcat gccaaaatta gactggatga
     1861 ggtgacgcgc attggtaaat gatggggaac gtatgacggt tttcaaacga agcaatacta
     1921 tccggaacag caaacacaag gtccagggca tctgtgatgt ggccacactt gccgtcaagc
     1981 agtccctgac gaaaacgctg ctggtttgtc tgggcattct gattgtgggt gtgtggttgc
     2041 tgatacaact tgtcatcgtt ccctttatgg ccgaactgaa ggatttgcgg gcacagacca
     2101 gccattcgct ctttgagaga aatcgggaaa tcatgcgctc cactgtggaa ctgggggcct
     2161 ccaatatcca gcaattgttt gctgctacgg aggtagcggt caataccatg attactgatc
     2221 ttcattcccc caaggtcaaa atggccaaga gcccgtttct gcccacagcg caggaatacc
     2281 tgacggcaga cgcgatcaaa aaatcgatga gccctcatcc cctgtatcct gatcgtttgg
     2341 tgagtctgga atatccccgt gtgtattatc ccagtgatga aatgtctgac gccagggcac
     2401 tggaggaaaa gaatctgcag ggatactggc cgtcgtttga gaaaactgtg agcagtctcc
     2461 attccctgac gggtgaattc aaatggttgt atgtggccag tgagacgggc atgttgttgg
     2521 tctatccggg aagcgataac gtgccgaagg gttacgatcc cagggtccgg ccctggtacg
     2581 tgggggcgat gggccgtcag gcaggatact ggacccctcc ctattacacg gcgggaggaa
     2641 atgaaattgt catcacgttt tcacaaccac tggggcaaca tgcttttatg aaaaacgccg
     2701 tggtggcggc tgacctggtg ttgacggata tcatcaacaa ggtgctgttc acacccttgt
     2761 gtgatgaatg caaaatctat ctggtaaatg aaagagatga aatcattgcc cagagggaac
     2821 cagccacact gaaggcatcg tggactgagc ctctgccggt caaaacgttg ggtgaggtct
     2881 tggcatcccg cgcagacgtg gtgggggagg ggaatgacgg gcaacacaat atcttcagcg
     2941 tgtctattga tcggttgcat tggcggttgg taggtgtgat ctacaatcac tcacttggta
     3001 atgacggggg acagatcatc gccaggatat catcattttc ccaaaaaaat aaaacagcct
     3061 tgatgattat catgggtgtg gtgcttgcca ttgttctgat cctcatgggg acgctgtatc
     3121 tgattgtcag aagaaccatg agaaccaaaa tggaaccttc cctggatcaa ttcaagaggc
     3181 tggcccaaaa cctggatgcg gttcatctgg acaacgtgct gagccttatg gcaggtgatg
     3241 cgacgaatgt gacagagagt ctgacgcacg aacataagat tgtggtggag gccctgcagc
     3301 gtctggggga caaactgctc aatgccgaaa aacagatcga aaccaaatcc aagctggcgg
     3361 ccgtgggaga aacggccgga caggtggcgc acgatatccg ttcaccgttg accgccctga
     3421 gcgtcatcgc ggcggaattg ggggaattgg ccgaggaaaa aagattgctc attcgctcgg
     3481 ctgtacaacg cattgaggat attgcgaatg atctatcatc aaaaagagtc caaagaccaa
     3541 agaccaatga ccaaagtcag aaacaagggg agattgtcat ccctgcgaaa gcagggatcc
     3601 agaatataac gccaactgtg gatcccagcc tacgctggga tgacaaaacg aatcctaccc
     3661 tcaagggcac aagcgatacg ctttctcttc cccccctagc cactaatagc cacctcctct
     3721 ccgctgttgt tgaatcattg gtgtcggaaa aacgcacaca gtatcgtaac cgttcaggtg
     3781 tgactatcga ggctaatctg ggcagtgagt cgtacggtct tttcgcaaac atacaactgg
     3841 atcagttcaa gagagtcttg tccaacattg ttaataacgc tgtggaggcg atggaaggcg
     3901 gcgggagttt ggtggttgct atgagagccg ttgagccaca gagccataga gcctatgatt
     3961 cagaatttac cgaggctcca acgccccatg gttccatggc tcaagacatt caaatcacca
     4021 tcatcgatac gggcaaaggc attcccgccg aaatcctgcc cagacttttt cggtgcgggg
     4081 aaagttttgg gaaaatagac ggtgccggtt tgggattgta tctt
//
LOCUS       DATDUN010000337         1255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_20268, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000337 DATDUN010000000
VERSION     DATDUN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20268"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VJC18_04705"
     CDS             <1..235
                     /locus_tag="VJC18_04705"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLD44713.1"
                     /translation="GVRMIFWPTFDLVLVIVLGILISLVGVIGDLCESLLKRGFGVKD
                     MGNMIPGHGGLLDRFDSLLFTAPLVYFITQLLP"
     gene            235..>1255
                     /locus_tag="VJC18_04710"
     CDS             235..>1255
                     /locus_tag="VJC18_04710"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLD44714.1"
                     /translation="MAEYQKKISLLGSTGSIGVSTLDLARQHPDRFQILALAAGSNTR
                     LLLEQIAEFRPRLVSVKSKQDYEEIKAQLSCDTEIFYGDDGAQRVATAEGADLVVSAI
                     VGAAGLKPTLAAIEAGKDVCLANKESMVIAGEIMSQKARQNRVRILPVDSEHSAIFQC
                     LNGEHPDDVDKLILTASGGPFWNLPQSAFIHITREQALKHPNWDMGAKITIDSATMMN
                     KGLEVMEAHWLFGMPVAKINVVVHPQSIIHSMVQFVDGSVMAQMGSPDMRVPIAYALM
                     YPRRITTGVKTLDLPKIEKLSFFEPDLEKFSCLRLAFDVARKGLSYPAVLNAANEVAV
                     DAFLKK"
BASE COUNT          282 a          297 c          349 g          327 t
ORIGIN      
        1 tggtgtgcga atgatttttt ggcccacctt cgatctggtc ctggttatcg ttttggggat
       61 cctcatttcg ctggttgggg tgattggtga cttgtgtgag tcactgctca aacggggttt
      121 tggtgttaag gatatgggaa acatgattcc tgggcacggc gggcttctgg atcgctttga
      181 ttccctcctg tttacggctc cgctggtcta tttcatcacg caattattac catgatggca
      241 gaatatcaaa aaaagatttc cctattgggt tcgaccggat cgatcggtgt ttccacactg
      301 gatctggcca gacaacaccc ggatcgtttt cagattttgg ccttggcggc tggttccaac
      361 acaaggttgt tgctcgagca aatcgcagag tttcggcccc gcctggtgtc agtcaaatcc
      421 aaacaggatt atgaggaaat caaggctcag ctttcgtgcg acacagagat attttatgga
      481 gacgatgggg cacagcgcgt ggctaccgca gaaggtgcag atttagtcgt ctccgccatt
      541 gtgggagccg ctggactcaa accaacattg gccgccatcg aagcgggcaa ggatgtgtgt
      601 ctggccaata aagagagcat ggtcattgcg ggtgagatca tgtcgcaaaa ggccaggcag
      661 aatcgggttc gcattttgcc tgtggacagc gagcactcgg ccattttcca atgcttgaac
      721 ggtgaacatc ctgatgatgt cgacaaactt attttgaccg cgagcggggg gcctttttgg
      781 aacttgccac agtcagcctt catccacatc accagagaac aggccctcaa acaccccaat
      841 tgggatatgg gggccaaaat cacgattgat tcggcaacca tgatgaataa aggtttggag
      901 gtgatggagg cccactggtt gtttggcatg cccgttgcta aaatcaatgt cgtggtgcac
      961 ccccagagca tcattcattc catggtacag tttgtggatg gttcggtgat ggcccagatg
     1021 ggttcgccag acatgcgcgt gcccattgcc tatgccttga tgtacccacg acgcatcaca
     1081 acaggtgtga agacgctcga tcttccaaaa atcgagaagc tttctttttt tgaaccggat
     1141 ctcgaaaaat tttcgtgcct gcgcctggcc tttgatgtgg ccaggaaggg cctgagttat
     1201 ccggccgtgc tgaacgcggc caatgaagtg gccgtggatg cctttttgaa aaagc
//
LOCUS       DATDUN010000338         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_515753, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000338 DATDUN010000000
VERSION     DATDUN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515753"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VJC18_04715"
     CDS             complement(<1..1034)
                     /locus_tag="VJC18_04715"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HLD44715.1"
                     /translation="MKLLLLQPPIEDFYDTDVRLQPIGLCYLKAVVKKHHPDIDVKVV
                     DLHHGWGKKTIPLPKELTYLKDYYPYKDKSPFCGFYQYYHFGASDDDILGVIEREAPD
                     VVGISSLFSPYYREVLHTAGLVKAVRDVPVIVGGSHVSADPLSLLQDPSVDFVICGEG
                     EKPLVEFLHSLKLKRGNTKIRWSHIPNLGYRKGTEYCINIERENYPIDEIPMPDLEDF
                     ESKNYSYFGKPLAFVITSRGCPHHCSFCSVHATFGFQYRKRAIDSVLDEIKKRYQAGY
                     RIINFEDDNLTFDKPFMKEFCQRLIDAFADKDITFVAMNGLSYLSLDDELLELMKKAR
                     FTHLNVALVTT"
     gene            complement(1075..2343)
                     /locus_tag="VJC18_04720"
     CDS             complement(1075..2343)
                     /locus_tag="VJC18_04720"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44716.1"
                     /translation="MDLSSIKILNPWWSDAQAIERDSHLLAVLKKNWYFDNPVKQTLA
                     FEPSHTYILRGPRQVGKTTLIKEKIFSALATKAIDANNIIFLSCEAMRDFQQLQEILV
                     SVLRLRNKEKVLLCLDEITFVKEWQRALLWAVNAGLLSNATTIITGSDAKDLKISSER
                     FPGRQVAEIAVHPLSFENYSHIACFDHLTEMELFDIYLRVGGFPHAVSDFAQYGQVTD
                     ETYEVYSNWIFGDAHKYGLSREILTHILYRIFDTVSSQVTWQGLIEKTPVHSHETAFA
                     YVEHLEQSFLCSVLRCFDPENERSSPRKAKKIYFVDPIIYAIAGALLRGVKNCYSWWL
                     QQINLDDFRGKIFEGVFINHVFRHTKKTPYYWYSRTLKREADLLIESEGQLFLYDAKL
                     KEQSVNTVLGRPVSVVTPQRFIKEKGMIIM"
     gene            complement(2535..>2932)
                     /locus_tag="VJC18_04725"
     CDS             complement(2535..>2932)
                     /locus_tag="VJC18_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44717.1"
                     /translation="LSNYQKKYKQVPVADVASKNAKMLGRMRDLPGMAGILDTDPNVV
                     AILNIVPATMYRRSDVLRLPKGRTLQIEVPAWGPGSTQTSNLGQGAYFVYEVLKMDES
                     WRTTDAHHYMVNAETKGPNKGKPIAYVPV"
BASE COUNT          812 a          660 c          676 g          784 t
ORIGIN      
        1 gttgtgacca aggccacgtt caaatgtgtg aaccgcgcct ttttcatgag ctccagcaat
       61 tcgtcgtcca gactcaaata cgaaagccca ttcatcgcca caaaggtgat gtccttgtcc
      121 gcaaacgcat cgatgaggcg ctggcaaaac tccttcatga agggtttgtc gaaggtgagg
      181 ttgtcatcct caaagttgat gatgcgatac ccagcttggt aacgtttttt gatctcgtcc
      241 aaaacactgt caatcgcccg cttgcggtat tgaaaaccaa aggtcgcgtg caccgagcaa
      301 aatgaacaat gatgcggaca accgcgagag gtgatcacaa aggccagtgg tttgccaaaa
      361 taagaataat tttttgattc aaaatcttcc agatcgggca tggggatttc gtcgatcggg
      421 taattttcgc gttcgatgtt gatgcaatat tccgtgcctt tgcggtagcc aagattgggg
      481 atatgcgacc acctgatctt ggtattgccc cgcttgagtt tgagggagtg caaaaattcc
      541 acaagcggtt tttcgccctc accacagatc acaaaatcaa cactcggatc ctgcaagagc
      601 gacaatggat cggccgaaac gtgcgagcca cccacaatca cgggcacgtc cctcacggcc
      661 ttgaccaagc ctgccgtgtg caggacctca cgataataag gagaaaacag gctcgaaatc
      721 cccaccacat cgggggcctc gcgttcaata acaccgagga tatcatcgtc actcgcgcca
      781 aaatggtaat actggtaaaa accgcaaaaa gggcttttgt ctttgtaagg ataataatcc
      841 ttgaggtaag tcaattcctt gggaagtggg atggtctttt tgccccagcc gtggtgcagg
      901 tcaacaacct tcacgtcgat atcggggtga tgttttttga ccacggcctt gaggtagcaa
      961 agcccaatgg gttgcaggcg aacatcggta tcgtaaaaat cctcaatggg tggttgcaag
     1021 agtaatagtt tcacaggcgc gtgtcatatt atgggagggg gactgggtca atgtctacat
     1081 gatgatcata cctttttctt tgatgaatct ttgtggcgtc accacagaga ctggtctgcc
     1141 caaaacagta ttgactgatt gctcttttag tttggcatca tacagaaata gctgtccctc
     1201 agattcaata aggagatcgg cctcgcgttt cagagtgcgg gaataccaat agtaaggtgt
     1261 tttttttgta tgacgaaaaa cgtgattgat gaaaacacct tcaaatattt ttcccctaaa
     1321 atcatcaagg ttgatttgtt gcaaccacca ggaataacaa tttttcactc ccctcagaag
     1381 cgcaccagca atggcataga tgataggatc tacaaaatag attttttttg ccttgcgggg
     1441 agaacttctt tcattttcag gatcaaaaca acggagcact gaacagagaa aggattgttc
     1501 caaatgctcc acgtacgcaa aagctgtttc gtgactgtga acaggcgttt tttctatgag
     1561 tccctgccat gtcacctgcg atgacactgt atcgaaaata cgatagagaa tgtgtgtcaa
     1621 tatctctcgg gacaggccat acttgtgggc atcaccaaaa atccagtttg aataaacttc
     1681 atacgtttcg tccgtgacct gtccatattg agcaaaatca gagacagcgt ggggaaaacc
     1741 gcctaccctc agatagatat cgaacaattc catttcagta agatgatcaa aacacgcaat
     1801 atgagagtaa ttttcaaaag aaagggggtg aacagcaatt tcggccacct gtcttcccgg
     1861 aaatcgttct gaactgattt ttaagtcttt ggcgtctgag cccgtgatga tggtcgttgc
     1921 gttcgagagc aatccggcgt taacagccca caatagagca cgttgccatt cttttacaaa
     1981 agtgatttca tcaagacaaa gcaggacttt ttctttattg cgtaaccgca aaaccgaaac
     2041 cagaatttcc tgaagttgct gaaaatccct catggcctcg caagaaagaa aaataatgtt
     2101 gtttgcgtca atggccttgg ttgccagtgc agaaaaaatt ttttccttga tgagagttgt
     2161 tttacccact tggcgaggcc cacgcaaaat ataggtgtgt gatggctcaa aagccagagt
     2221 ctgttttaca ggattgtcaa aataccagtt ttttttcaac acggccaaca gatgagaatc
     2281 tctttcgatg gcctgcgcat cactccacca aggattgagt atttttattg aacttagatc
     2341 cataacaata tgatattaaa tcatattact atcttttgtc aattacgcga ttgtaatcgc
     2401 actaatttgc cagtgggtca ataggttatg ggttatgggg aacggataat gggtttggtc
     2461 aatggcgatc gaggtcaaaa aaaaaccaga cagggccttg cacgagaccc tgtctggcat
     2521 tgtgttgatt ttcatcaaac aggaacgtaa gcaatgggct tgcctttgtt ggggcctttg
     2581 gtttcggcat tcaccatgta gtgatgggca tcggtagtac gccaagattc gtccatcttc
     2641 agaacttcat aaacaaagta agcgccctga ccgaggttag aggtctgtgt tgaaccggga
     2701 ccccatgcag gcacttcgat ttgcaaggtc ctgcccttgg gcagtctcaa cacatcactt
     2761 ctgcgataca tggtggcagg tacaatatta agaatcgcaa ccacatttgg atcggtatca
     2821 agaattccag ccataccagg caagtcacgc attctgccca gcatctttgc attcttactc
     2881 gcaacatcgg ccaccggcac ctgtttgtat ttcttttgat agttggacag tt
//
LOCUS       DATDUN010000339         1815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_57883, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000339 DATDUN010000000
VERSION     DATDUN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57883"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VJC18_04730"
     CDS             <1..533
                     /locus_tag="VJC18_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44718.1"
                     /translation="SLFENRAYVPPLDLGTFWATFADVYARSTHKRSNLFDNRSPNEI
                     ATILKHSILKTLSPAEALLLERSTKTTIGIVIRGCLGHGVVSDFGHHWYNLFREGDTF
                     VIMNGQVDSQTNIVALEDSQVAILSPNFATEIKSDDPVLAARLSMSTLAIIQQFIAEM
                     EEGSVDVENETIAIQL"
     gene            complement(554..970)
                     /locus_tag="VJC18_04735"
     CDS             complement(554..970)
                     /locus_tag="VJC18_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLD44719.1"
                     /translation="MDISEFSIPDKLYFRIGEVAKLIGVEPYVLRYWETEFSEIAPTK
                     SRSQQRLYRRQDVELICLIRDLLYKSHFTIKGAKQKLKDKMAGREVAKAQLAAEHVPV
                     QLPLGVAGGALTEPAREAIKAVIEEMQEFLDTPQKM"
     gene            complement(980..1300)
                     /locus_tag="VJC18_04740"
     CDS             complement(980..1300)
                     /locus_tag="VJC18_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HLD44720.1"
                     /translation="MTDNVLSPEGDKTKTLTKAELIESIYEKVGFSKKEASDIVELVF
                     ETLKATLEKGEKVKISGFGNFVVRQKRPRIGRNPQTGQEIEISSRRVLTFKPSQVLKT
                     SLNK"
     gene            complement(1425..>1815)
                     /locus_tag="VJC18_04745"
     CDS             complement(1425..>1815)
                     /locus_tag="VJC18_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44721.1"
                     /translation="LLTEHKLREKVFYAELDYDFFASNYQKQPRAYRPIGQLPSVYRD
                     LSLVIPKEMTYTEVQTVIEKYRPELLGTYEIFDLFQGGNLGDDKKSLTLSMIYEPKGE
                     SLTDEQVNGVHFELVEKLKKELGVELR"
BASE COUNT          448 a          471 c          408 g          488 t
ORIGIN      
        1 tcagtctatt tgaaaatcgg gcctatgttc caccactcga tctgggcacg ttctgggcca
       61 cctttgctga cgtgtacgcg cgtagcaccc acaagcgatc gaacctcttc gataatcggt
      121 cgccaaatga aattgccacc atcctgaagc actccatcct caaaaccctg tcccctgcgg
      181 aagcgctact gcttgagcgt tccaccaaaa caacgatcgg cattgtcatc aggggttgtt
      241 tgggacatgg tgtggtcagc gacttcggac atcactggta caatcttttc agggaaggcg
      301 acacctttgt gatcatgaat gggcaagttg attcgcaaac gaacatcgtc gccctggaag
      361 acagtcaagt ggccatcctc tcacccaact tcgccacgga gatcaagagc gatgaccctg
      421 tgctggccgc acgcctgagc atgagcacac tcgccatcat ccagcagttc atcgcggaaa
      481 tggaagaggg atcggtggat gtggagaatg agacaatcgc aattcaactt tgaaaatgaa
      541 gaaataagcc tcttcacatc ttttgaggtg tatccagaaa ctcctgcatt tcctcaatca
      601 cagccttgat cgcctcgcgt gccggctcag tcaatgcccc cccagccacg cccaatggca
      661 actgcacagg gacatgttca gcggcaagct gcgctttggc cacctcgcga cctgccattt
      721 tatctttgag cttctgtttg gcacccttga ttgtgaaatg acttttgtag agcagatcgc
      781 gaatcaggca gatcagctcg acatcctgac gtcggtataa cctctgctgt gaccgcgact
      841 tcgtgggggc aatttcggaa aattctgtct cccaatagcg aagaacatac ggctccacac
      901 cgatgagctt ggccacctca ccaatgcgaa aataaagttt gtcgggtatg ctgaattcgc
      961 tgatgtccat cggtgacctt tatttgttca acgaggtctt aagcacctgc gacggtttga
     1021 aggtcagtac gcgccgggaa gagatttcga tctcttgtcc tgtttgggga ttacgtccaa
     1081 tgcgcgggcg tttctggcgg acgacaaaat tgccaaaacc agaaattttt accttctcgc
     1141 ccttttccag tgtggccttg agggtttcga ataccagctc aacgatgtcg gaggcctctt
     1201 ttttggaaaa accgaccttt tcgtaaatgg actcaatcag ttcagccttg gttaaggtct
     1261 tggtcttgtc gccctcaggt gacagcacgt tatcagtcat atcttctcct tattttttac
     1321 tttaagtatg caagaaatta tgccggtcga atatgtcttt tttagataag atattctaaa
     1381 actcaactca agaaaaaaca atcgttttct gtaaccctta tacctcaccg taactcaacc
     1441 cccaactcct ttttgagttt ctcgaccagc tcaaaatgaa ccccattgac ctgttcatcg
     1501 gtcaaggact ccccttttgg ttcatatatc atcgataatg ttaaggattt tttatcatca
     1561 ccaagattcc ccccctgaaa caggtcaaag atttcgtaag tgcccagtag ctccgggcga
     1621 tatttttcga tgactgtctg tacctcggta tacgtcattt cttttggaat cacgagggac
     1681 aggtcgcgat acaccgaagg gagttggcca attggccgat aggcacgcgg ctgtttttgg
     1741 tagttgctgg caaaaaaatc ataatccaat tcagcgtaaa aaactttttc gcgcagtttg
     1801 tgttctgtca aaagc
//
LOCUS       DATDUN010000340         1651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_432374, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000340 DATDUN010000000
VERSION     DATDUN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432374"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VJC18_04750"
     CDS             complement(<1..1085)
                     /locus_tag="VJC18_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLD44722.1"
                     /translation="MNQKIAITGDWDEGLELVTKELHKSIFEPLLVQAPDLKAVLKRK
                     QSDVFLFCVKDGSGSVTGQINEIRRSNEGLPIFVFSKTVDVPFIVACIKGQVNDFFVR
                     PLDRHVLLNKIEKEVHLYQLTQKVFMGSSSSSDDSRFENMIGRSRCMKENFQMISVVA
                     KSNATVLITGESGTGKELVAKAIHRRSDRAPNRFVDINCGAIPKELLENELFGHEKGS
                     FTGAHKRYQGSFEVAHQGTLFLDEISEMDVMLQVKLLRALQERTITRIGSNENIKIDA
                     RIIAATNRNLQKNIEEGRFREDLFYRLNVVNIDIPPLRERREDIPLLAMFFLEYCSAK
                     NNRIFLDFANDAMEALINYDWPGNVREL"
     gene            complement(1126..>1651)
                     /locus_tag="VJC18_04755"
     CDS             complement(1126..>1651)
                     /locus_tag="VJC18_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44723.1"
                     /translation="SHSLSQAVKDGFMREDLYNILSHAQWHIPSLSERVEDLPLLVAY
                     HLRQGPHQEVRHYFSRDAMTALCQHEWDHNVAELFASVDAIVQQASQTMVSAHMLPNR
                     ILQRSFYRTQAQSANVFEQTYNEAKEKIVADFTRAYFHDLLAKVDGNLTVAAEQAGMD
                     RSNLKKLIKRYGLL"
BASE COUNT          399 a          442 c          394 g          416 t
ORIGIN      
        1 agttcccgga cattgcccgg ccagtcgtag ttgataagcg cttccatggc atcgttagcg
       61 aaatccagaa aaatccggtt gtttttggcc gaacaatatt ccagaaaaaa catggccaag
      121 agggggatgt cttcgcgtct ctccctcagc ggtgggatgt cgatgttgac cacgttcaac
      181 cggtaaaaca ggtcctcgcg aaaacgtccc tcctcaatgt ttttctgcag attgcggttg
      241 gtggcggcga tgatcctcgc atcaatcttg atattttcat tactgccaat ccgtgtgatc
      301 gttctctctt gcagagccct gagcaacttg acttgtaaca tcacatccat ttcgctgatc
      361 tcatcgagaa aaagagtgcc ctgatgcgcc acttcaaagc taccctgata tcgtttgtgc
      421 gcacccgtga acgatccttt ttcgtgccca aacagttcgt tctcgagcaa ttctttggga
      481 atcgcgccac aattgatatc gacgaaacga ttcggcgccc tgtcggaacg acggtgtatg
      541 gccttggcca ccagctcttt tcccgttccg gattccccgg tgatcaacac cgtggcattg
      601 gacttggcca caaccgagat catctgaaaa ttctccttca tgcaccggga acgaccaatc
      661 atgttttcaa aacgagaatc atcggaagag gaggaactgc ccataaacac cttttgggtg
      721 agttggtaga gatggacttc cttttcgatc ttattcagca agacatgacg atccagagga
      781 cgcacaaaaa aatcgttcac ctgaccctta atgcaggcca caatgaaggg aacgtcaacc
      841 gtcttggaaa acacgaaaat gggcaggcct tcgttgcttc tcctgatttc gttgatttga
      901 cctgtcacag aacctgatcc gtctttgaca caaaaaagaa acacatcgga ctgtttcctt
      961 ttgagcacgg ccttgaggtc cggggcctgc accaacaaag gctcaaaaat tgatttgtgt
     1021 aactctttgg tcacgagttc aagtccctcg tcccagtctc ccgtaatggc aattttttga
     1081 ttcatcatca ttgatcctaa cccggcctgg ccggaaaata acccatcaca acagcccata
     1141 acgctttatg agctttttga gattactgcg gtccatcccc gcctgctcgg cagccacagt
     1201 gaggttgccg tcaaccttgg ctaacaagtc gtgaaaatag gcccgcgtga aatcggcgac
     1261 aattttttcc ttggcctcgt tataggtctg ttcaaacaca ttcgcacttt gggcctgtgt
     1321 cctgtaaaac gatctttgca ggattcgatt cggcaacatg tgtgcagaga ccatggtttg
     1381 gctggcttgt tgcacaatgg catcgacact ggcaaacagc tccgccacat tgtggtccca
     1441 ctcatgctgg cacagcgctg tcatggcatc gcgcgaaaaa taatgccgca cctcctggtg
     1501 agggccctgc ctgaggtggt aggcaaccaa caagggcagg tcctcaacgc gttcgcttaa
     1561 ggagggaatg tgccactgcg cgtgactgag gatattgtaa aggtcctcgc gcatgaatcc
     1621 gtccttcacc gcctgcgaca gggagtgaga g
//
LOCUS       DATDUN010000341         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_351242, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000341 DATDUN010000000
VERSION     DATDUN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351242"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VJC18_04760"
     CDS             <1..462
                     /locus_tag="VJC18_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44724.1"
                     /translation="MNKTTRQQLLLSVIKREVITSQEELLLGLQQKGAKTTQATLSRD
                     ICELGIIKKGGRYHCPKPHNTSLFFGHVQSLEPVTGSFLIVQTTSGLANGVAEWIDQQ
                     KLPEVMGTIAGDNTILIALKSSESSQNLIKKFSKLFSNTSSRSRRSRSSSI"
     gene            638..1387
                     /locus_tag="VJC18_04765"
     CDS             638..1387
                     /locus_tag="VJC18_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44725.1"
                     /translation="MNCSQLNIDIVDSNHSGTFDLSEDSLWINDQYVSGPELTNLMTA
                     LSVTEDDFSALGWFDYGLYQADNLLTEKVTTFFKKKYDELPSYVASSTAIVPEDCLSV
                     TNPAFKHDLGAFLNQHPLFFQAKTQTHTFKQSIGHRCEDGCTAVDGLVVGSTLYHAIV
                     MPENTGGVVLTFPVKENVSLMEHDSLTESEPPFGYQTQTLCVGHDGTPATYLVQEEKY
                     AHEMMGLPYLLQQHGAMLSIVSGLGPSECQE"
     gene            complement(1521..>2234)
                     /locus_tag="VJC18_04770"
     CDS             complement(1521..>2234)
                     /locus_tag="VJC18_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HLD44726.1"
                     /translation="IFSEKKQHDNKAINTSNDIVLSDNTFWGKLKSGMWVGLADMVDH
                     TGPWILFGLALAASIQPYLEQSRIFYAIPPAWQVPMFALLGMPIYICASGITPFVAVL
                     IYHGVSPGAAIAFMLTGPATNATTFGILAQLHGKKIAITFAALVMLASCSVGYIINFF
                     HITIPKILPLEHQHAFPAHTIAFFVMLTALYAFSLLRCGPQAWARKIIAGDVPAMEVH
                     EDCKDEEAPHEHGHCGCGG"
BASE COUNT          579 a          522 c          572 g          561 t
ORIGIN      
        1 atgaacaaga caaccaggca acaactcctt ctttctgtca tcaaaagaga ggtcatcacc
       61 tcgcaggaag aactgcttct tgggcttcag cagaaggggg ccaaaaccac acaagccacg
      121 ttatcgcggg acatctgcga attgggtatt atcaaaaaag gtgggcggta tcattgtccc
      181 aaaccccaca acacttcact gttctttggc cacgttcaat ctttggaacc ggtcacgggt
      241 tcgtttttga tcgtgcaaac aacgtcgggt ttggccaatg gggtggccga atggatcgat
      301 caacaaaaac tgcccgaagt tatgggcacg atcgctggcg acaacacgat tctcatcgct
      361 ttaaaatctt cagagtcatc gcaaaatctg atcaaaaaat tttccaaact tttttccaac
      421 acatcgtctc gttcacgtcg tagccgttcg tcctcaatct gaccgaaact atcggcagtt
      481 ggcgaacggc aagtttattt caatcaaaat catttgaatt gtagtttatt ataaactttt
      541 tttatcacag gttgtgaggt catgacttgt gaatgaaaaa accgtgggca catttgccca
      601 cagccctgag cggtgtcgaa gggcaaagga gacgtgtatg aactgttcac aactcaacat
      661 tgacattgtt gattccaatc acagcgggac ttttgattta tcagaagact ccctctggat
      721 taacgatcaa tatgtttcag ggccagagct gacaaatctg atgacggcac tctcggtgac
      781 ggaagatgat ttttcagcgt tgggctggtt tgattatggt ctgtatcagg ctgacaacct
      841 tttgacggaa aaagtcacca cttttttcaa aaaaaagtat gatgaactcc catcttatgt
      901 tgcatcgtcc acggccattg ttccagaaga ttgtctgtcg gttacaaatc ctgccttcaa
      961 gcatgatctg ggggcttttt taaatcaaca cccgctgttt tttcaggcaa aaacacaaac
     1021 gcataccttc aaacagtcta tagggcacag atgtgaggat gggtgtacgg ctgtggatgg
     1081 tctggtggtg ggtagtaccc tctatcatgc gattgtcatg cccgaaaata ctggtggggt
     1141 cgtgctgact tttccggtga aggaaaatgt ctcgttgatg gagcatgatt cgttgacaga
     1201 gtctgaaccg ccgttcggct atcagacgca aacattgtgt gtagggcatg atggcactcc
     1261 tgccacatat ctggttcagg aggaaaaata cgcacacgag atgatgggct tgccctattt
     1321 gttacaacaa cacggggcca tgctgagtat tgtcagtggg ctagggccat cggaatgcca
     1381 agagtagaaa caacaagacc aatcaatgca ggggccgggt tatctggccc ctgcaatttt
     1441 taatatcctc aagtgaaggg acacttttcg gtctaaacgg tcgtcctctc gtccttcgtc
     1501 ctttcgtgtt ttcgtccccc ctatccccca cacccacaat gaccgtgctc gtgcggcgct
     1561 tcttcatctt tgcaatcctc gtgtacctcc atggcgggga catctcccgc gatgatcttg
     1621 cgggcccagg cttgtgggcc acaacgaagc agagagaagg cgtacaaggc ggtgagcatg
     1681 acaaagaagg cgatcgtatg agcggggaaa gcatgctgat gctccaacgg caggatcttg
     1741 ggaatcgtga tgtgaaaaaa attgatgatg taacccacgc tacaggaggc gagcatgacg
     1801 agtgccgcga atgtgatggc gatttttttg ccatgcaatt gggccaatat accgaacgtc
     1861 gtggcgttgg tggcagggcc tgtcagcatg aaggcaatgg ccgcgccggg tgagacacca
     1921 tgataaatta acacagcgac aaaaggggtg atgcccgagg cacagatgta aataggcatg
     1981 cccagtagcg cgaacatcgg aacctgccag gcagggggaa tggcgtaaaa gatgcggctt
     2041 tgttccagat aaggttggat ggatgccgcc aaggccagcc caaacaatat ccagggacct
     2101 gtgtggtcaa ccatgtcagc caggcccacc cacatgccgg atttgagctt cccccaaaag
     2161 gtgttatcgc tgagcacaat gtcgttgctg gtgttgatcg ctttgttgtc atgttgtttt
     2221 ttttcggaga agat
//
LOCUS       DATDUN010000342         3539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_516173, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000342 DATDUN010000000
VERSION     DATDUN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516173"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1295
                     /locus_tag="VJC18_04775"
     CDS             285..1295
                     /locus_tag="VJC18_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HLD44727.1"
                     /translation="MVKLISHLLVALVFFVALVGAVVYYAWLPTGTMTIHNEIVSQDP
                     LSLKKPDQRIKQLTVVTYNIGYAYGLENNKGGRLTKSDVTRNLLQMIDALRQSNPDIL
                     FLQEVDFHSRRTYDINQAVLIANALNMPYSAHSLTWDHTYLPWPFWPISSHYGRVVSG
                     QVVMSRFPIVSQDVLVFDKPQSNPFWYNWFYLDRVVQKITLLINNQRYSVFHVHLEAF
                     DEKARQQQTARLSRWVSENRETALLAAGDFNSTTNIGPSLSIREANELLASKVFLKTL
                     EKETLLTNAETANLLTYPSDLPQMKLDHIFYSSKLKPVKTTVLNSNASDHAAVLSTFK
                     LK"
     gene            complement(1695..3242)
                     /locus_tag="VJC18_04780"
     CDS             complement(1695..3242)
                     /locus_tag="VJC18_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HLD44728.1"
                     /translation="MFARIFSAGLFGVEAYLINVEVDIRMSQLPQWSTVGLADSAVKE
                     SKDRVIAAIRNAGYDFIFRRVTINLAPADTKKDGTAFDLPIAIGLMAASGNLHTKLIN
                     DSLFIGEMSLTGDLRPIKGVLPMAILARDLKISNIILPKENQGEAGMVKGLNVLGFAH
                     LSEVVGFLTGQFKIEKTEPNNEFEETLPRAWSRDFSDIYGQYQAKRAIEVAAGGGHNI
                     MLSGPPGSGKTMLASRIPTILPPLTFEESLETSKIYSVMGLLKRKHQLLSQRPFRDPH
                     HSVSNAGLIGGGTFPRPGEVSLAHNGVLFLDELPEFQKNVLELLRQPLESHEVCISRS
                     AASLTYPARFILVASCNPCPCGYLGHPKIGCSCTPQQVQRYRAKLSGPLLDRIDLHVD
                     VPPVAYEDMRRRNNNVETSCVVAKRITKVRGIQRKRFLGSDLYLNSQMTTKLIEKHCE
                     LDVAGEKILKSSMEKFHLSARAISRILKVSRTIADLEESEAIKMDHLLEAIQYRSFDR
                     MAGNNAF"
BASE COUNT          931 a          890 c          743 g          975 t
ORIGIN      
        1 atcggctgtg ctttcgagtt tgataatttc tcgcgcttga gtcttttttg ccataacatc
       61 tccttttaac acgccatagc atcccacaac accggcacat gcctggcgcg tagcgaaggc
      121 ggtttttaac gtcgacaatg caacggggcg gctgtatagc gtcaacagat caggaaagtc
      181 aattcaaggg aatatcgtgt atcatttcat gcgagtatcc ctatgccaca ataacagact
      241 gtaccaaaac ctgttttaac aaatcttttg gataggtttt ttatatggtg aagctcatat
      301 cgcacttact cgttgccctc gtttttttcg tagcgcttgt cggtgccgtt gtctattacg
      361 cctggttacc aactggcacc atgacaattc ataacgaaat cgtttcgcaa gatcccctct
      421 ctttaaaaaa acccgatcaa cgcatcaagc agctgacagt tgtcacatac aacatcggct
      481 acgcctatgg tctggagaac aataaagggg gacggctgac caaaagcgat gtcacgcgta
      541 acctgttaca aatgatcgac gctttgcgac agtcaaatcc tgatatttta tttttgcaag
      601 aagtggattt tcactctcga cgcacgtatg acatcaatca agccgtactc atcgccaacg
      661 ccctcaacat gccttactca gcacacagcc tcacttggga ccacacatac ctcccgtggc
      721 ctttttggcc aatctcctca cactatggcc gggtggtttc gggtcaagtg gtcatgagcc
      781 gatttcccat cgtgagccag gacgttcttg tcttcgataa acctcaaagc aatccttttt
      841 ggtacaattg gttttatctt gatcgcgttg tgcaaaaaat caccctgctc atcaacaatc
      901 aacgatattc tgtctttcat gttcatctgg aggcgttcga cgagaaggcg cgccaacaac
      961 aaaccgcacg gctatcccgt tgggtatcag aaaatcggga aaccgcgttg ctggcggccg
     1021 gtgattttaa cagcacgact aacattggtc cctcgttgtc aattagggag gcaaacgagt
     1081 tgctggcctc aaaagttttt ttaaaaaccc tcgaaaagga aaccctttta acaaacgccg
     1141 aaacggccaa cctgctcacc taccccagcg acctacctca aatgaaactg gatcacattt
     1201 tttattcctc caaactcaag cctgtaaaaa cgactgtcct gaattcaaac gcaagtgacc
     1261 acgccgccgt cttgtccaca ttcaaactaa aatagcatga taattttttc tgagaatatt
     1321 ttgacaccac attcaaaaca acagcctgtg gataaggcac aaactacttt attatgaatt
     1381 acgcgctgtt acaaaacatt gtccacaacc acagacaagc ttgctttgac cacacaataa
     1441 ccaagtcgac tcaaggagtt aggcttggag gctttgccat acacaacaac gcttctgtgg
     1501 ataagttttt tgtaatgttt tttcctcaat gattccgaga atgaaaaaaa tgtcattttt
     1561 ttttgttgtc tctgagaaga aaaaaacatt taatgggcca tggcatagcg ccagataaag
     1621 attgacagag cacaaagacc cggcgtcggt aggggcactt atgaagcgcc cttacaggcc
     1681 cgggcctttg tgttttaaaa cgcattgttt cccgccatgc ggtcaaaact gcggtattga
     1741 atcgcctcca acagatgatc catttttatt gcctcacttt cttccagatc ggcaatcgtc
     1801 cgcgaaacct tgagaatccg gctaatcgcc cttgcggaca aatgaaattt ttccatggat
     1861 gactttagga ttttttcgcc agcgacatcc aactcacagt gtttttcgat gagcttggtt
     1921 gtcatctgtg aattcaaata aaggtcgctt cccagaaaac gttttctttg aatcccccgc
     1981 acttttgtaa tgcgcttggc cacaacacat gaggtttcaa cattgttgtt gcgtcgcctc
     2041 atatcctcat aagccacggg tggtacatct acatgaagat caatccggtc cagtaacggt
     2101 ccggaaagtt tggctcggta gcgttgtacc tgctgtggcg tgcaggaaca tccaattttg
     2161 gggtgtccca aatacccaca gggacaaggg ttgcaggagg ccaccaatat gaaacgtgcc
     2221 ggatatgtta aactcgcagc ggaacgcgag atgcaaacct cgtggctttc taatggttgc
     2281 ctcagcaact caagaacatt tttttgaaac tcaggcagtt cgtccaaaaa tagtacccca
     2341 ttatgggcta ggctcacctc tccgggacga gggaatgtgc cccctccaat caaccccgca
     2401 ttggatacgg aatgatgggg atccctaaaa ggtctttgtg acagcaactg gtgttttctt
     2461 ttgagaagcc ccatcacact gtatattttc gatgtctcca gagattcctc aaatgtgagt
     2521 ggaggcagaa tggtgggaat ccgagaggcc aacatcgttt tgcctgatcc tgggggacca
     2581 ctcaacatga tattatggcc accaccagcg gccacctcga tagccctctt ggcctggtat
     2641 tgaccgtata tgtcggaaaa atccctgctc caggctcgcg ggagtgtttc ttcgaattca
     2701 ttattgggtt ccgtcttttc tatcttgaat tggcccgtga gaaaacccac aacctcactc
     2761 aaatgagcaa acccaagtac attcaggcct ttgaccatgc ccgcttctcc ctgattttcc
     2821 tttggtaaga tgatgttgct tatcttaaga tctctcgcca gtatggccat cggcaacact
     2881 ccttttattg gtctgaggtc tcctgtcaga ctcatctctc caataaaaag agaatcatta
     2941 ataagcttgg tgtgcaaatt acccgaagcc gccatcaacc ctatggcaat gggcaaatcg
     3001 aaagcagtac catccttttt tgtgtcggct ggtgccagat tgattgtgac acgacgaaaa
     3061 ataaaatcgt aacccgcatt gcgtatagcg gctatcaccc tatccttgct ttctttgacc
     3121 gctgaatcag caagtcccac tgtgctccat tgaggcaatt gcgacatacg aatatcgacc
     3181 tcaacattaa taagataggc ctcaacccca aacaaccccg cactgaaaat tcttgcaaac
     3241 ataatcgctc ccattccgtg ttaccgtttt ccattcaatt ttcatgccaa aggcttcatg
     3301 ttttagtgtt cgattatcac ttgctatttt ttttatctct cacaataaag atcgtgcgtc
     3361 ccgtccgtgg gacgcattgt gggacgaata gagagtttga gtgggatttg cagcgggact
     3421 ttttggccaa atttgacctt ttgtaaactg tgtgctaacc ggcctctcca atttttggtt
     3481 gttctttttt tacatccgct aggtagcagg tgggtgaggg ggcgacacaa gaaaatgga
//
LOCUS       DATDUN010000343         2470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_599512, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000343 DATDUN010000000
VERSION     DATDUN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599512"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VJC18_04785"
     CDS             complement(<1..1202)
                     /locus_tag="VJC18_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD44729.1"
                     /translation="MIKLSGISLSHLLPWRMAKIIRDWAQKLRALLDQNERLADANQR
                     PDLADQLSQVVQAVVVQQSERTADYQRQKQQLESVVNALSDAVILTDAQHVLILANPA
                     WYRLFDSSPVCLGQPLLQSVRIPELFDGLTHTQKTGLTSTIDFDYLGRFFTARISKIS
                     VSTLDGCFTVLSDVTQIKNAEQVRRDFTANVSHEMKTPLTSILGYSETLMQGALGDPP
                     TANAFLQIIHDQAVKLKDLIDDVLHLARIESPAWKVEKQPVDLAKVVNEVVNEFRKHP
                     KQLSLSTNEFNVTVVSDAQAIKLILHNLIDNAVKYTPTGGQVSVTVKEDPPKGVVLIV
                     QDTGVGISTNEQSRIFERFYRVGGHSTKEGTGLGLAIVKHLVEKLGGTITVTSTVGKG
                     SAFSLNLP"
     gene            complement(1199..1885)
                     /locus_tag="VJC18_04790"
     CDS             complement(1199..1885)
                     /locus_tag="VJC18_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD44730.1"
                     /translation="MSDIILIIEDDPSIAQLLTYNFQKEQFDVKHTVSGEDGLSLLTQ
                     KIPSLIILDLMLPGICGLDACRIIKSEPKTKNIPIIMLTAKSEEMDKIIGLETGADDY
                     VTKPFSVRELILRVKAILKRSTKSTPQKAVLVFKDLTLDSNEYRVTLGKQKIDLTLTE
                     FKLLLFLLSNPNKIFSRDRLLNRVWGYQSDVFSRTVDTHITRLRSKLGSHGQYLVSVR
                     SVGYRWEGAT"
     gene            1884..2159
                     /locus_tag="VJC18_04795"
     CDS             1884..2159
                     /locus_tag="VJC18_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44731.1"
                     /translation="MPTNLLNIFFYCNTIEFTVVLTEEALNGNLQPHYELAILGKTVC
                     YPTKLDHELNRALYATRGELAVVMGKTVGVNCFYEEQTRLDGALDPG"
     gene            complement(2182..>2470)
                     /locus_tag="VJC18_04800"
     CDS             complement(2182..>2470)
                     /locus_tag="VJC18_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HLD44732.1"
                     /translation="DQCYVGTCPELMIGGVHGKNETRVYNELCKVVDDWIKIYQKDNE
                     PLPEPVIHKDYSGKFILRIGKELHKVLAIKALLEGVSLNHFCMKLIKSKLA"
BASE COUNT          655 a          542 c          589 g          684 t
ORIGIN      
        1 cacggcaggt taaggctaaa cgccgaccct ttccccacgg tgctggtgac cgtgatggtg
       61 ccacccagtt tttcaaccaa atgtttgacg atcgccagac caaggcccgt tccctctttt
      121 gtggagtgac ctcccacgcg ataaaatctt tcaaaaatgc ggctctgttc atttgttgaa
      181 attccaacgc ccgtgtcttg aacaatcaag acgacaccct tgggtggatc ttctttgacg
      241 gtgacggaaa cctgtccgcc tgtgggtgta tatttgacgg cattatcgat cagattgtgt
      301 aagatcagct tgatggcttg tgcatcggaa accacggtga cgttaaactc atttgtcgat
      361 agggataatt gttttggatg tttgcgaaat tcattcacaa cttcattcac aaccttcgct
      421 aaatcaacgg gctgcttttc caccttccac gccggagatt caattcgggc caaatggaga
      481 acatcgtcga tcaaatcttt gagcttaact gcttggtcat gaatgatttg cagaaaagcg
      541 tttgcggttg gtggatcacc cagtgcgccc tgcatcaagg tttcggaata acccaagata
      601 gatgtgagtg gtgttttcat ttcgtgcgaa acattggccg taaaatcacg acgaacctgt
      661 tcagcatttt tgatttgggt cacatcgctt aagactgtga aacaaccatc cagtgtggag
      721 actgatattt ttgagatgcg tgcagtaaag aatcgaccca aataatcaaa atcaatcgtg
      781 ctcgtaaggc ctgttttctg cgtgtgtgtg agaccatcaa aaagctcggg gatccgaacg
      841 gactgcaaca gaggctgacc caaacacaca ggagacgagt cgaagaggcg ataccaagcc
      901 ggattcgcca agatgagcac atgttgagca tcagtgagga tgacggcatc tgacaaggca
      961 ttcacaactg attcaagttg ttgcttttgt ctttgatagt ctgctgttct ttcactttgc
     1021 tgaacaacga cagcctgcac aacttgagag agctggtcag caagatcagg gcgttgattc
     1081 gcatcagcga gcctttcgtt ttgatcgagt aaagcccgaa gcttctgtgc ccagtcacga
     1141 ataattttag ccattcgcca cggcaaaaga tgagaaagag agatgcctga gagcttgatc
     1201 atgttgcacc ttcccatcga taacccacac tacggaccga tacaaggtat tgaccgtgtg
     1261 atcccaactt gctgcgtaag cgcgtgatgt gcgtatccac cgttctggaa aagacatcgg
     1321 attggtaccc ccacacgcgg ttcagaagtc tgtcgcgcga aaaaatcttg ttgggattcg
     1381 aaagcagaaa gagaagaagt ttaaattctg tgagtgttaa atcaattttt tgtttaccca
     1441 aagtcacgcg atattcattt gaatcaagtg tgaggtcctt gaaaacaagc acggcttttt
     1501 gtggagttga ttttgtactg cgcttcaaaa tggccttcac acgcaagatg agttctcgaa
     1561 cagaaaaagg cttggtcaca taatcatcgg ctcccgtttc cagcccaatg atcttatcca
     1621 tctcctcgga ttttgccgtg agcatgatga tagggatatt tttggtttta ggttcagatt
     1681 tgatgatcct gcaggcatcc aagccgcata ttccaggaag catcagatca aggatgatga
     1741 gactgggtat tttctgcgtg agcaaagaaa ggccgtcttc accggaaacg gtgtgtttaa
     1801 catcaaattg ttctttctga aaattgtagg tcaataattg agcaatactc ggatcgtctt
     1861 cgatgatgag aataatatct gacatgccaa caaacctatt gaacatattt ttttattgca
     1921 acacaattga gtttacagtt gtgctgactg aagaggccct gaatggcaat ttgcaacctc
     1981 attacgaact ggctattttg ggcaaaaccg tgtgttaccc caccaagctt gatcatgagc
     2041 tgaatagggc tttgtacgcc acacgagggg aattggctgt tgtgatgggc aaaacagtgg
     2101 gtgtgaattg tttttatgaa gagcagaccc ggctcgatgg tgcgttggat ccgggctaga
     2161 gaaagatgag gcgcatagtc atcaggcgag ttttgatttt atgagcttca tacaaaaatg
     2221 atttaggctg accccctcaa gcagggcctt tatggccaag accttgtgaa gttcttttcc
     2281 gatacgcaga ataaatttac cggagtaatc tttgtgtatt acgggttcgg gtaaaggctc
     2341 gttgtccttt tgatagattt tgatccaatc atccacgacc ttacaaagtt cattgtaaac
     2401 ccgggtttcg ttttttccgt gcacgccacc gatcatcagt tccggacatg tgccgacata
     2461 acactgatct
//
LOCUS       DATDUN010000344         1707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_472948, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000344 DATDUN010000000
VERSION     DATDUN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472948"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..838)
                     /locus_tag="VJC18_04805"
     CDS             complement(125..838)
                     /locus_tag="VJC18_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLD44733.1"
                     /translation="MVIKQIKCACEQCDAFEHSVFRSLTADESRLFEDSKICNLYKKG
                     QVIFHEGNRPQGLYCVKDGNVKVFKTGVDGREQIVRMAKKGDVLGYRTLIGEEAYTAT
                     AAAIVDSQICLIEKQVFFDILRTNNEMLWSFLHLLANELRNAENFIADLAQKTVRERL
                     AEVLLLLKNKYGCQPSDNQTINVVLTRDELASIVGTATETLIRHLSDFKDEQVIEVKG
                     RKIKILNTKKLFHIANINL"
     gene            995..>1707
                     /locus_tag="VJC18_04810"
     CDS             995..>1707
                     /locus_tag="VJC18_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44734.1"
                     /translation="MPKPVVSKFSSAASHFKKLLDQLKDEMGFIAHSSAETFEVYREK
                     ITQNWNVKWPETLNNYKAALKELPKGLRQRDFVQTAHAEIEKFEQELNRFSEQIKNES
                     LDDWRRTFNKQLTQANEFMGRFQASIKRMDEVVAELEENTLVQKFLRGEPVFSCRTKL
                     QWRRKLFHAFMGLSFLYLFVYAGLAPSTKNTIAVLFIGTAIATEILRAHSKWFGNLVC
                     RLFGPIMREHEKTKVTAAT"
BASE COUNT          423 a          446 c          375 g          463 t
ORIGIN      
        1 acgatgccct tggcaaaata gcgcgtgtcg gccacacacc aaccggacga cacagacttt
       61 ttcttgaggt agttcccttt gagatagtct gtgagcacct caatgaatat agagacgacg
      121 tctttcatag attgatgttc gcgatgtgaa aaagtttctt ggtgttgagg atcttgattt
      181 tgcggccctt gacctcgatc acctgttcat ccttgaaatc cgacaggtgc ctgatgaggg
      241 tctcggtggc cgtgcctaca atgctcgcca attcgtcacg cgtcaacacc acgttaatcg
      301 tttgattgtc gctgggttgg cacccgtact tgtttttgag caggagcaac acctcggcca
      361 acctttcgcg gaccgtcttc tgggccaggt cggcaataaa attttcggcg ttgcgcaatt
      421 cattggccaa caggtgtaaa aacgaccaca acatttcgtt attggtcctc aggatatcga
      481 aaaacacctg cttctctatt aagcagatct gcgaatccac aatggccgcg gccgtcgccg
      541 tgtacgcttc ttcgccaatg agggtccggt agcccaacac atcgcccttt ttggccatgc
      601 ggactatctg ctcgcgacca tccacacctg ttttgaagac cttcacgttc ccgtctttaa
      661 cgcagtacaa accctgtgga cgattcccct catgaaaaat gacctgtcct tttttgtaga
      721 gattgcagat cttggagtcc tcaaacaatc gggactcgtc tgccgtgaga cttctaaaaa
      781 cagagtgttc gaatgcgtca cattgctcac aggcacattt gatttgtttg atcaccatag
      841 gttcgcggga aattatgttg tttgttccca aaaggcaagc ttcttcattt tttcttcatt
      901 ttttgattgt ttcattgttt cattgcttga ttgtttcatt gtttcactct ctttcttctt
      961 tattatcttg atcctatttg ataacaaaac cttcatgccc aaaccagttg tcagcaagtt
     1021 ttcctcagcg gcctctcact ttaaaaaact cctggaccaa ctcaaggacg agatgggctt
     1081 tatcgcccat agctcagccg aaacctttga ggtctatcgc gaaaaaatca cccaaaactg
     1141 gaatgtcaaa tggcccgaaa ccctcaataa ttacaaagca gccctcaaag aattacctaa
     1201 ggggctccgt caacgtgatt ttgtccagac tgcccacgcg gaaatcgaaa aattcgagca
     1261 ggaattgaac cggttctccg agcaaatcaa aaacgaatct ttagatgatt ggaggcggac
     1321 ttttaacaaa caattgaccc aggccaatga attcatgggc cggttccagg cctcgattaa
     1381 acgcatggat gaggtcgtgg cagaactcga ggaaaacacg ttggtgcaga aatttttgcg
     1441 cggagagccc gtgttttcgt gccgcaccaa actccagtgg cgccgcaaac tgtttcatgc
     1501 cttcatggga ctgagttttc tttatctgtt cgtttacgcg ggactcgccc cgtcaaccaa
     1561 aaacaccatt gccgtgcttt tcatcggcac agccatcgcc accgaaatcc tgcgcgcgca
     1621 cagcaaatgg tttgggaacc tcgtctgtcg attatttggt cccatcatga gggaacacga
     1681 gaaaaccaag gtcaccgcgg ccacaca
//
LOCUS       DATDUN010000345         5128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_142359, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000345 DATDUN010000000
VERSION     DATDUN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142359"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /gene="nadE"
                     /locus_tag="VJC18_04815"
     CDS             <1..591
                     /gene="nadE"
                     /locus_tag="VJC18_04815"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HLD44735.1"
                     /translation="MQINIDPLHKVYEKFFHKIFPQGVSDLTEQNIQARIRANLLMAI
                     SNNSERLLLNTSNKSELAMGFGTMYGDLCGAIAVISDLTKTQVYELCGHLNLQKEIIP
                     KNILKKAPSAELKPDQKDSDTLPPYDKLDPLLDVLINQYGQGVDFDSCDDLTRKVMNT
                     IMKNEWKRFQAPPTLRISNKAYGAGRRIPVVGKLRI"
     gene            complement(588..1226)
                     /locus_tag="VJC18_04820"
     CDS             complement(588..1226)
                     /locus_tag="VJC18_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44736.1"
                     /translation="MKKSATKKGVKKTVLSVNRVRSTKQRNEKAKKGSLNGSESVAHY
                     NAILIEELKSDFKFVAEQVMGLHDVIKQEVRQVRDDLGARIDNVESVLTYHSKLLEEN
                     KAEHEQMRAENKAEHQQMKSENAAEHAQIRAEFMTALENLRAENKAEHEQMRAENQAE
                     HEQMRAENNAEHERMMLENTAEHERIESKLDSLIVVVSRHETVIEEIQSAVI"
     gene            complement(1322..2248)
                     /locus_tag="VJC18_04825"
     CDS             complement(1322..2248)
                     /locus_tag="VJC18_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44737.1"
                     /translation="MHTANISNMVLTALSGVGTGKADKAMNFVAGLMTLVADPQLTGQ
                     VLLTHIEQFLQTQKTPLGDRDMVRLFTAVKTHLGENPELLTQMLLSRENKTKISRVSS
                     KTSADEGGETAKVVARDVSQNDPAANHDWASSRHSPNGQLAHVPVVCQTPAELINHPP
                     NSKTAHKKSPRRLSADPFGLLLCEKIYPPFLIKIKKLLARASEQNVTVRVLDAYRDDP
                     KGGSFHNYGLAVDIDFAVTADTNKTLLVLNKIILMNLAEAQGLFVRSSLNPTATHLHF
                     EYPHAVTISECQQAWDQCELEAVWALLSKKKL"
     gene            complement(2291..3619)
                     /locus_tag="VJC18_04830"
     CDS             complement(2291..3619)
                     /locus_tag="VJC18_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HLD44738.1"
                     /translation="MQTINQVNQNYAVRLEVGKNDNLETSFIDGKYLIRGSSADDQIE
                     AMATTIDGKKMVNIVVNGQLLDTVSYTQMQSLLVQGEAGNDNITIHSGLLLDVKLLGK
                     GGNDVIMGGDGNDFIDAGDGNDTVEAGKGNDVVLAGSGDDTISGGDGKDKIMGNEGSD
                     VLNGGVGDDFIEGGEDTDVITGGGGNDEIYGNTKPAFIIPKPIKSPVPTAPVVTQKPV
                     VINPITNPVPPCKPAPSPTLAPISTTNPMPTDPSAPVSLVNGVLRINGTNANDEIKVT
                     KTTSGYLVTINGKQTRISDQNVGKIIVNGQVGNDNIEIANDIALKTVLNGGSGNDTIK
                     GGGGADYIAGGSGNDWVEGGAGDDHLRGNDGNDVLKAGAGNDHVYGNGGDDALIGWSG
                     NNTLDGGDGNDIYSGNKLKDKFDVGNGTDKINGVGLTPTVTIFGKYSLKV"
     gene            complement(3641..4132)
                     /locus_tag="VJC18_04835"
     CDS             complement(3641..4132)
                     /locus_tag="VJC18_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44739.1"
                     /translation="MDVLMAEEQKSYSEQIKNNRAMKNAHFQKRMNMLDNNIDKLKDS
                     QSQTRKSGFFNAIVGVVGSLLSQAAQYLNTVVPGLGTAVSTALSGITKLLQSQDPFAK
                     NAGEAQIAAEDYKKLAEYEGNRSQLYADDAESARSSKQAVTQRISKSIDNQQDSGSVA
                     VSC"
     gene            complement(4207..4734)
                     /locus_tag="VJC18_04840"
     CDS             complement(4207..4734)
                     /locus_tag="VJC18_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44740.1"
                     /translation="MITTNSTNSIDIEGLANNVMNDLSDGDINDKKVIKACIQSERNA
                     LKTKRAERIKNLEEQLRGVAGGGGKCIKILKPIAKVLDFISKPLSLLSLGKLDIGLGS
                     ALKNIEEAKKQGKLTGLKIDEQGIMRAIEGVKKYLTDNVASLDSQEQSVQQQVNTILK
                     MLDDIGNSVHQVGNV"
     gene            complement(4752..>5128)
                     /locus_tag="VJC18_04845"
     CDS             complement(4752..>5128)
                     /locus_tag="VJC18_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44741.1"
                     /translation="IKFDGKNEKDAVTLNSDEKEYLQDDKEFVGDVETGEVEDVCKTK
                     TTDFESQKVKGTSHTHVSKEVIEKKIEIYKQKLDSLNEQTELMSVSLQSLTNQRKICF
                     ETISNIVKKQEDCVGTIVRNIG"
BASE COUNT         1238 a         1305 c          964 g         1621 t
ORIGIN      
        1 atgcagatca acatcgatcc gttgcacaag gtgtatgaaa agttttttca caagattttt
       61 ccgcaaggcg tgtccgatct gaccgaacaa aacatccagg cccgtattcg cgcgaatctg
      121 ctcatggcca tctcgaacaa ctcagaaagg ttgctcctca acaccagcaa caagtccgaa
      181 ctcgccatgg gtttcggtac catgtacggc gatctttgcg gggccattgc cgtgatttcg
      241 gatctgacca aaacacaggt ttacgaactt tgtggccacc tcaatctgca aaaggagatc
      301 atccccaaaa acatcctcaa aaaggcgcca tcagcggagc tcaaacccga tcaaaaggat
      361 tccgataccc tgccacccta tgacaaactc gaccccttgc ttgatgtgtt gatcaaccaa
      421 tacggccaag gcgttgattt cgattcgtgt gacgacctga ccagaaaggt gatgaatacc
      481 atcatgaaaa acgagtggaa gcgttttcag gcaccaccca cgctcaggat ttctaacaag
      541 gcctacggcg cggggcgccg cattcctgtg gtgggcaaat tgcgcattta gataactgcg
      601 gattgaattt cttcgatcac tgtctcgtgg cgactcacaa ccacaatcaa agaatccagt
      661 ttggattcaa tgcgctcatg ctcagcagta ttttctaaca tcatcctttc atgctcggca
      721 ttattctcag ctctcatttg ttcatgctca gcttgattct cagctctcat ttgttcatgc
      781 tcggccttat tctcagccct cagattttca agagcagtca tgaattctgc tcttatttga
      841 gcatgttctg ccgcgttttc agacttcatc tgttgatgtt cggccttatt ctcggctctc
      901 atttgctcat gctcggcttt attctcttcc aatagtttgc tatgataggt aagcaccgac
      961 tcaacattat caattctggc ccccagatcg tctctgactt gtctgacttc ctgtttgatc
     1021 acatcgtgca aacccatgac ctgttcagcc acaaacttga aatcggattt aagttcttcg
     1081 atcagaatgg cattgtaatg ggcaacggat tctgatccat tcagtgaacc ttttttcgct
     1141 ttctcatttc tttgttttgt tgaccgaacc ctattgacag ataatacagt ttttttgacg
     1201 cccttttttg tggcagactt tttcataaca ctcccaacaa ttttcttgtt tacgcttacc
     1261 ggcctactta atgaatttcg gggtgtcaac atgtgatagg caacttgcga aattcattaa
     1321 gctataattt ttttttagaa agcaacgccc aaactgcctc tagttcacac tggtcccacg
     1381 cctgttgaca ttcgctgatc gtgactgcgt gagggtattc aaaatgcaaa tgcgtcgctg
     1441 tgggattcaa actactcctc acaaaaaggc cctgtgcttc tgccaaattc atcaaaatga
     1501 ttttattgag caccaaaagt gttttgttgg tgtctgcagt gactgcaaaa tcaatatcca
     1561 cagccaaccc gtaattgtga aatgagcctc ccttggggtc atctcgatac gcatccaaga
     1621 cgcgaacagt cacgttttgc tccgatgctc tggcaagcag ttttttgatc ttgatcagaa
     1681 aaggcggata gattttctcg catagcaaca gcccaaaagg gtcggcagac aaccggcgag
     1741 gggatttttt gtgagctgtt ttgctgttcg gtggatgatt gatcaattcg gctggggtct
     1801 ggcaaacaac aggcacgtgc gccaattgcc cattgggaga atgtctggac gaggcccagt
     1861 cgtgatttgc agcgggatcg ttttgtgata cgtctcgcgc caccaccttg gcggtttcac
     1921 caccctcatc ggctgatgtt tttgatgaca cacgactgat tttggttttg ttttctctgg
     1981 acaaaagcat ctgtgtcaat aactccggat tttcgcccaa atgagttttg actgcggtaa
     2041 agagcctgac catatcgcga tcgccaagcg gtgtcttctg ggtttgtaaa aattgttcaa
     2101 tgtgtgtcag caaaacctga ccggtcagtt gcgggtcagc cacgagggtc atgagccccg
     2161 ccacgaagtt catggcttta tcggccttcc ccgtccccac ccccgataag gccgtgagaa
     2221 ccatgttgga aatgtttgcg gtgtgcatac tgattattgc cagttcactc tttgcaagag
     2281 acacccttcg ctacactttc agcgaatact taccaaaaat ggtcactgtt ggggttaatc
     2341 ctactccgtt aattttgtct gtaccgttcc ctacgtcaaa tttgtccttt aacttattac
     2401 ctgaataaat atcattgcca tctccaccat ccagcgtatt attacccgac cacccaatca
     2461 gcgcatcatc tcctccattt ccatatacat gatcattgcc agcacctgct tttaatacat
     2521 cgttcccatc atttcctcgt aggtgatcat ctccagcgcc accctcgacc caatcattgc
     2581 cgctcccacc tgcaatataa tcggctccac caccaccctt gatcgtatca tttccactac
     2641 caccattgag tactgtcttg agtgctatat cattagctat ctcaatatta tcgttaccaa
     2701 cttgaccgtt cacaattatt ttgcccacat tctgatcaga aatgcgcgtt tgctttccat
     2761 ttattgttac aagataacca ctcgtggttt ttgtgacctt gatttcatca ttggcattcg
     2821 taccattgat cctgagcaca ccgtttacca gcgaaaccgg cgcggatgga tccgttggca
     2881 ttggattggt tgtagagatc ggtgccaatg tgggtgacgg tgctggctta caagggggaa
     2941 ccggattcgt tataggattt atcacaacag gtttttgagt tacaaccggt gctgtaggca
     3001 ctggcgattt aattggcttt ggtattataa atgctggttt tgtatttcca taaatctcgt
     3061 cattaccccc acctcctgtg atgacatctg tatcttctcc gccctcaatg aaatcatcgc
     3121 ccactcctcc attcaatacg tcgctccctt catttcccat tattttatct ttgccatctc
     3181 cgcctgatat ggtgtcgtcg ccagaaccag ccagcacaac atcattgcct tttccagcct
     3241 cgacagtatc attgccatca ccagcatcga taaaatcatt tccatcccca cccataataa
     3301 catcattgcc acctttaccc agaagtttaa catccagcag taacccacta tgaattgtaa
     3361 tattatcatt gcctgcttca ccttgaacaa gcaaactctg catttgtgta taggaaactg
     3421 tatcaagcaa ttgaccattc acaacaatgt tgaccatttt cttgccgtca attgtagtag
     3481 ccatcgcctc aatttgatca tctgcgcttg atccccttat gagatatttc ccatcaataa
     3541 aggatgtttc aaggttatca tttttcccca cttcgagtct cactgcgtaa ttctggttca
     3601 cttgattgat tgtttgcata atatgatcct ctctccattg ttaacaacta acggccacag
     3661 aaccactatc ctgctgattg tctattgatt tactgattct ctgcgttacg gcctgtttgc
     3721 tggagcgtgc tgactcggca tcatcggcgt ataactgaga tcgatttccc tcatattctg
     3781 cgagtttttt ataatcctct gccgcaatct gtgcctcccc cgcatttttc gcaaaggggt
     3841 cctgggattg aagcaacttc gtaatgcccg acaaggctgt actgacggct gtacctagcc
     3901 ctggcacaac tgtatttaaa tactgggcgg cttgagacaa tagacttcca acaacgccga
     3961 caatcgcatt gaaaaaacca gatttgcgtg tttgactttg ggaatcttta agcttgtcga
     4021 tgttattatc gagcatgttc atacgctttt gaaagtgcgc gtttttcatt gcccgattat
     4081 tttttatttg ttcagaatat gacttttgtt cctccgccat caaaacatcc acattcgtcc
     4141 cttgaccacc tacggtcatc cctgaccctg ctgctactgt tgatattgac atacagtctc
     4201 cttgttttaa acattaccca cttgatgaac actgtttcca atgtcatcca acatttttaa
     4261 aatggtatta acctgttgtt gtaccgattg ttcttgagaa tccaaagacg ccacattgtc
     4321 tgtcaggtac tttttaacac cctcgatagc cctcataata ccctgttcat caatttttaa
     4381 tcccgtcagt ttcccctgtt tttttgcctc ttcgatgttt tttaaggcac ttcctaaccc
     4441 aatatccaat ttaccaagcg ataataggga gagaggtttg gagataaaat caagaacctt
     4501 ggcgattggc tttaaaatct taatgcattt gccaccacct cctgcgactc ctcgcagttg
     4561 ttcctcaaga tttttgattc tctccgcccg tttcgtttta agtgcatttc tctcggattg
     4621 aatacaagct ttgatcactt ttttgtcgtt gatatcgcca tcgctcaaat cattcataac
     4681 attattcgcc aatccttcga tatcaattga gtttgtgctg ttagttgtaa tcatgattac
     4741 ctccccatac attatccaat attcctgact atcgtaccga cacaatcctc ttgctttttc
     4801 acgatattag aaatggtttc gaaacagatt ttgcgttggt tggtcaatga ctgcaaactc
     4861 acactcatca attctgtttg ttcattaagt gaatcaagtt tctgtttata gatctcgatt
     4921 ttcttttcga taacttcctt ggaaacatgt gtatgggaag tccccttgac tttttgagac
     4981 tcaaaatctg ttgtttttgt tttgcaaaca tcttcaactt ccccagtttc tacatcgcca
     5041 acaaattctt tgtcgtcctg aagatattcc ttttcatcgg aattgagggt cacagcatct
     5101 ttttcgtttt tgccatcaaa tttgattt
//
LOCUS       DATDUN010000346         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_351515, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000346 DATDUN010000000
VERSION     DATDUN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351515"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VJC18_04850"
     CDS             <1..1234
                     /locus_tag="VJC18_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HLD44742.1"
                     /translation="MKPRDLLPSVDDITEKAKKIFRFEPEDTTEKALKERMGKKPIAR
                     VYTDEPRSLIPPAPLEEAPNGFAQSNVYESLLSEIKPQHLIDRDEPVRSLQPSRSYFE
                     QVAEDDLSITQSEMPRRKAAQVGFWDKVFLTLGMVKFQHSIFALPFALTSVLVASQGR
                     PGWLNVLLVIVAMITARNCAMTFNRIVDRHFDKKNPRTRDRAIPSGQLSLKFAVIFGT
                     INLIAFIVIAGLFNSLTLALSPLALAIILGYSLTKRVTHYTQFYLGFALGCAPMAAWI
                     AMTGKLAVFPAVLGLAVLFWVAGFDIIYATQDYEFDCRHKIKSLVVKYGIRRSLMMSR
                     VIHVLAVISFVLAGALQGRHLFYYLGCLLTASLLAYEHSIIKPYDLSRVNLAFFTLNG
                     YVSLLFFVFVLLDVILLA"
     gene            complement(1184..>2047)
                     /locus_tag="VJC18_04855"
     CDS             complement(1184..>2047)
                     /locus_tag="VJC18_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD44743.1"
                     /translation="LLLLPYRAKWAIMLVFCALMALTTFNRIHTFRDSVALWSDTCQK
                     ISPSQLNQPSLYKPFFYLGNHLAQAGQYSRAIEAYQTSIQVQPLLAPAYFNMGLAYFK
                     ISDYQKAIASLLKASALSPENYSIYFHLGNTYQMLQDHAQAVKFYNMSINLNSVHLGS
                     HVQLGVSLFESDQLDAAFEKFQKLIKLDSKNGQVHDYFGRVLLKKNQLPEALESFRIA
                     HELLPNDKPICFHIAQIEWTLGNKEQALLFVQKALDDNPNFVEAKTLLGEITTSRQQD
                     DVQENKHKKKQ"
BASE COUNT          454 a          500 c          568 g          525 t
ORIGIN      
        1 catgaaaccc agggatttgt taccatcggt cgacgacatc accgaaaagg ccaaaaagat
       61 cttccggttc gaaccggagg acaccaccga aaaggccctc aaggaaagaa tgggcaagaa
      121 acccatcgcg cgggtataca cggacgaacc tcgttccctg atacctcctg cgccacttga
      181 agaggcacct aacggttttg cgcagtcgaa cgtgtatgaa tctctgctgt ccgagatcaa
      241 accgcaacat ttgatcgatc gtgatgaacc tgtccgatca ctccagccct cacggtctta
      301 ttttgaacag gtggcagagg atgatttgtc catcacacag tcggaaatgc ccagacggaa
      361 ggccgcacag gtaggttttt gggacaaggt gtttctcacg ttggggatgg tgaaattcca
      421 gcactcgatc tttgccctcc cgtttgctct cacctctgtg ctggttgcct cgcagggaag
      481 gccgggctgg ctgaacgtgc ttctggtgat tgtggccatg atcacggcac gcaactgcgc
      541 catgaccttc aatcggattg tcgatcgaca cttcgataaa aaaaatcccc gtacgcgtga
      601 tcgggccatc cccagcggac aattgtcact gaagttcgcc gtgatttttg ggaccatcaa
      661 cctgatcgcg ttcattgtga tagcaggctt gttcaacagc ctgacgctgg ccctgtcgcc
      721 gctcgcgttg gcgatcattc tgggctattc gctgaccaag agggtgacac attacacgca
      781 gttttattta ggctttgccc tggggtgcgc gcccatggcg gcctggatcg ccatgacggg
      841 aaaactggcc gtctttcccg ctgtgctggg actggctgtg ctgttttggg tggcggggtt
      901 cgacatcatc tatgccacgc aggattacga atttgactgc cgacacaaaa tcaagagtct
      961 ggtggtcaaa tacgggattc gccggtcgct catgatgtct cgggtaattc atgtccttgc
     1021 cgtgatcagt tttgttttgg ctggggccct gcagggcagg cacctgttct attatctggg
     1081 ttgtctgctc acagcctcgc ttttggccta tgaacacagc atcatcaagc cgtacgatct
     1141 gtcacgcgtg aacctcgcgt ttttcaccct caatggctac gtctcactgc ttttttttgt
     1201 gtttgttctc ttggacgtca tcttgctggc gtgacgtggt gatctcaccc agcagggtct
     1261 tggcttcaac gaagttggga ttgtcatcca acgccttctg tacaaagagc agggcctgtt
     1321 ctttgtttcc cagagtccat tcaatctgtg caatgtgaaa acagatgggt ttgtcgttgg
     1381 ggagaagctc gtgagcgatc ctgaaggact ccagggcttc tggcaactga ttttttttga
     1441 ggagaaccct gccaaaatag tcgtggacct gtccgttttt tgagtcgagc ttgatgagct
     1501 tctggaattt ttcgaaagcg gcatccagtt gatctgactc aaacaaagac acccccagtt
     1561 gtacgtgcga acccaaatgg acggaattga gattgatgct catgttgtaa aacttgacgg
     1621 cctgcgcgtg atcctgaagc atctggtagg tgtttcccaa atgaaagtag atggaataat
     1681 tttctggcga tagggccgac gccttaagca gagaggctat ggccttttga taatcgctga
     1741 tcttgaaata agccagaccc atgttgaaat aggcgggggc cagcaggggt tgcacctgaa
     1801 tggaggtttg ataggcctca atggcgcggc tgtattgacc cgcttgcgcg aggtggttgc
     1861 ccagataaaa aaatggtttg tacagcgagg gttgattcag ttggctggga ctgatttttt
     1921 ggcatgtgtc ggaccacagg gcgacagaat ccctgaacgt atgaatgcga ttaaatgtgg
     1981 tgagggccat gagggcgcag aagaccagca tgatggccca tttggcccgg tatggcagaa
     2041 gcagaag
//
LOCUS       DATDUN010000347         1013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_432748, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000347 DATDUN010000000
VERSION     DATDUN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432748"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..666
                     /locus_tag="VJC18_04860"
     CDS             139..666
                     /locus_tag="VJC18_04860"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017352169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HLD44744.1"
                     /translation="MLSIIVAIAKNNCIGINNQMPWHIPEDFAHFKKITMGHTVVMGE
                     KTFESIGKPLPGRKNIIITLLKNYHPEGVDVIHGLDELFALAQGDDEVFVIGGATIYK
                     LTLPYADRLYLTRVNITLDGDAFFPQIDFENDFEIVEEGDVLFSAKQGLEYQFVVAER
                     AIAPPHNSADTSMPM"
     gene            complement(597..>1013)
                     /locus_tag="VJC18_04865"
     CDS             complement(597..>1013)
                     /locus_tag="VJC18_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44745.1"
                     /translation="LSTLDSYLFIAGTTISYDLMPFRFRNRRLTHNLGMIFAGVVSIL
                     LGVFFEGNIKNVWKTFGSYFSACFLVPIVLSYVFPKRIGDRAFVAITIITAIVVTAWR
                     LWERQGFWQEVDELYIGMLVSALLCGGAMALSATTN"
BASE COUNT          284 a          252 c          252 g          225 t
ORIGIN      
        1 aagccgcctg ttccggtgcc gccgcgaggg aagcctgctg aaccaacaac gagcgccacg
       61 caggcaacaa caccttcctt ggccaatctg cccaagccat cgcgaccgac aggggcgagg
      121 gatagaaaga agtaacgaat gttgtccatc atcgtagcca ttgccaaaaa taattgtatc
      181 ggtatcaaca accagatgcc gtggcatatc ccggaggatt ttgcccattt caaaaaaatc
      241 accatgggtc acactgtggt gatgggcgaa aaaacatttg aatccattgg caagcccttg
      301 cccggacgca aaaacatcat catcacgctc ctcaaaaact atcatcctga aggcgttgac
      361 gtcattcatg gccttgatga gttgtttgct ttggcccagg gtgacgatga ggttttcgtg
      421 atcggtggtg ccaccatcta taaactcacc cttccttatg ctgaccggct gtatctgaca
      481 cgcgtgaaca ttacattgga tggcgatgcg tttttcccgc agattgattt tgaaaatgat
      541 tttgaaattg tcgaagaagg tgatgtcttg ttttcggcaa aacagggact ggagtatcag
      601 tttgtggtgg cggagagggc catcgccccc ccgcacaata gcgccgatac cagcatgccg
      661 atgtagagtt cgtcaacttc ttgccaaaag ccctgtcgtt cccaaagacg ccaggcggtc
      721 accacgatcg cggtgatgat ggtaatggcg acaaaagccc tgtcgccgat gcgtttggga
      781 aaaacgtagc tcagaacaat cggtaccaaa aaacatgccg agaaataact gccgaaagtt
      841 ttccagacgt ttttgatgtt gccctcaaaa aacacaccaa gcaaaattga aaccacaccg
      901 gcaaagatca tgcctagatt atgggtcagg cgcctgttgc ggaacctgaa aggcatgagg
      961 tcataagaaa tcgtggtgcc ggcaatgaac aaatatgaat caagcgttga cag
//
LOCUS       DATDUN010000348         1966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_391436, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000348 DATDUN010000000
VERSION     DATDUN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391436"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..>1966
                     /locus_tag="VJC18_04870"
     CDS             159..>1966
                     /locus_tag="VJC18_04870"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HLD44746.1"
                     /translation="MEASLLPYQSWMNLDAIVRSLYRRFISHRQLLEWSTVQMTGRSA
                     QKQVSQFYLSVGVSSVCTSFFVMGSLYDKPNHLIQASPWLGVWLFSPLVAWFLNRRPA
                     VKQPDSFISDDDRQFLRRISRRTWRYFSDFVNEQSSWLPPDNYQVSHRNQLALRTSPT
                     NIGLWMVSVLGARHFGYVTTDDVVHKLSKTFETLAKLERYEGHLLNWYDIQTLAPLPP
                     RYVSTVDSGNFLGCLWTLKKGLEELVLLPLLDDKAVEGIRDAYEILKEYVTSETRGCF
                     IVDDLDSLLHNWNTSRKYAGDVLAILWKTKTIVTHLKQKTSAFPGTPGNTNYWVGALL
                     NQLESWRDTADRYLGWIEILNEKTDEDILRLDKNILPEFHSALTQAPSLVALAKGQVS
                     VIKSLESVRSQMATQDQARLAWIDRVLIAFSRSKWLAGEALAFTQRLTLECEQLSESI
                     NMHFLYNPERKLFSIGYNVSEGRLDRSFYDLLASEARLGSFIAIARGEVPVAHWFDMT
                     RPYGAVGRHRVLLSWTGTMFEYLMPFLFQRSYGNTLLDKAAHEAVAIQIAYANKHRVP
                     WGISESAFGDLDIHKTYQYHAFGVPSLGLKRGQEET"
BASE COUNT          453 a          489 c          489 g          535 t
ORIGIN      
        1 ggttgctatc gtattgggtt ggtttcatcg ccagcgcctt tgtgattgta cagtttatgt
       61 tccactctct cgcccagcat ttcacctggg ccacgacgcg gcaggggttc aaaagcgttt
      121 ccttttcaag gattgtggcg gatattgctc gagtcgttgt ggaagcctct ctcctgcctt
      181 atcaatcctg gatgaatctg gacgcgatcg tgcgcagtct ttacagacgt tttatttcgc
      241 accgccagct tctggaatgg agtacagtac aaatgactgg gcgcagtgcc cagaagcagg
      301 tttcccagtt ttacctttca gtgggagtca gcagtgtttg cacctctttt tttgtgatgg
      361 gatctcttta tgacaaaccc aatcacctga tccaggccag tccctggttg ggtgtgtggt
      421 tgttttcccc acttgttgcc tggttcctca atcggcgacc tgctgtcaaa caacctgact
      481 cattcatttc cgatgacgat cgacaattcc taagacgcat ttcgcgacgc acctggcgct
      541 acttttcgga ctttgtgaat gagcaatcct cctggttgcc gcccgataat tatcaggtct
      601 ctcaccgaaa ccaactggct ctgcgtacca gtccaacgaa cattggcctt tggatggtca
      661 gtgttttggg tgcgcgtcat tttggatacg tcacgacaga cgatgtcgtg cacaaattgt
      721 caaaaacctt tgaaaccctg gccaaattgg aacgctatga aggtcacctt cttaattggt
      781 acgatattca aaccttggct cccttgcccc cccggtatgt ttccacagtc gatagcggga
      841 atttcctggg gtgcctttgg acccttaaaa aagggctcga ggaactcgtt ttgctccctt
      901 tgctggacga caaagccgtt gagggtattc gtgatgctta tgaaatattg aaagaatatg
      961 tgacaagcga aaccaggggg tgttttattg tggatgacct tgattctctt ctgcacaact
     1021 ggaacacgtc acgaaaatac gccggggatg tgctggcgat cctttggaaa acaaaaacaa
     1081 tagttactca tctcaagcag aagaccagcg cgttcccagg cacaccgggg aacacaaact
     1141 attgggttgg tgcgttgctc aatcaacttg agtcctggcg ggacacagct gatcgctatc
     1201 tgggctggat cgaaatcttg aatgaaaaaa ctgatgaaga catcctccgt ctggataaaa
     1261 atattctgcc cgaatttcat tctgcgctca cccaagcccc gtcactcgtg gccttggcca
     1321 agggtcaggt gtccgtgatc aaatcactcg aaagtgtacg aagccaaatg gcgacccagg
     1381 atcaggcaag acttgcctgg attgatcgcg tcttgatcgc tttcagcaga tcaaaatggc
     1441 tggcgggaga agctctggcg ttcacgcaac gactcaccct ggaatgtgaa caactttccg
     1501 agtccatcaa catgcatttt ttgtataacc ctgagcgcaa attattctcg ataggttaca
     1561 atgtttcgga agggcgattg gatcgctcct tttatgatct attggccagc gaagcgcgtt
     1621 tgggtagttt cattgccatc gcacgcgggg aggttcccgt tgcgcattgg ttcgacatga
     1681 ctcgtcctta tggcgctgtt gggcgtcacc gggtattgct cagttggaca ggaaccatgt
     1741 ttgaatacct gatgcctttt ctttttcaac gctcgtatgg gaatacgttg ctcgacaaag
     1801 ccgctcacga agctgtcgcc atacaaattg cctacgccaa caaacaccgg gttccgtggg
     1861 gaatctcgga atcggctttc ggtgatctcg acattcacaa gacgtatcag tatcatgctt
     1921 ttggagtacc gagtctggga ctcaaacgtg gacaagaaga aacgat
//
LOCUS       DATDUN010000349         2676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_58343, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000349 DATDUN010000000
VERSION     DATDUN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58343"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VJC18_04875"
     CDS             complement(<1..1480)
                     /locus_tag="VJC18_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44747.1"
                     /translation="MKLSKFISVLCLLVMLCLPPLNTQAFEFSAHGYYRTRFEYSHDL
                     DLQRPNAGIVPGDANNASNDRFGTIAFAQQRFRLNPKLKINDNISLYGQIDFLDNLLM
                     GQSDVTTLHIFNPVVGTVNMSPANGPYGVIGPIGGDILGNGGGNVNVRRIWADILTPA
                     GLFRIGRQPSHFGLGILNNDGDSFDGDFGDTYDRIMYMAGLNFNNGSRLNFGMAYDFA
                     YEATTDPSLSGLTSGVSSNWNDVMQGGAIIMFSSDHVEFGIFGGARFRDGNEGGYTTT
                     AAYVDNCADDGRPGDYVCPDPADTTDPAYDVDNDGQVDDLIDLPAGVDGDTLVYMGDV
                     YARFHFARDYTLALEGVYMGGKLAPGIAIDAIALDADSQAGITNPLTSPIELPLNGTQ
                     NDLQIIMGAMEFDAEWPFGGEVHIQSGYASGDQSPLSQTITQLGFRPDYDIALILFDQ
                     PIGTSPALVIGGVTELGRVPVSPNYVNNAIYGTLEYKQEFDIT"
     gene            1626..>2676
                     /locus_tag="VJC18_04880"
     CDS             1626..>2676
                     /locus_tag="VJC18_04880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HLD44748.1"
                     /translation="MNKITITPLGGLGRTGALNCMLYETAKTAILVDCGAGFVDDGYP
                     GVDILIPNFDLLETKRDKLKAVILTHGHEDHVGGLPYLLQEICVPVYATPFTRGVITS
                     KISEFKIKGVQLHELQPSHKIQIGDFAIEPVFIHHSIMDVVAFYITVADQKIFHCTDF
                     KLDDSAPAQKQMDLDRFAAIGKAGVDLALLDSTNVLAEGTSESEAVIKDNLYEIFSSV
                     PGRLIACLFSSNTYRLQSLLQCARQAGRKVALTGRSTKEYFRIAASLDRLDLAGIDFH
                     DVEDIGQFADQEVFVIVTGSQAEPRSVLSRMSRDMFRPFKIREGDTLLMSSRMIPGNE
                     GRILHMLNNIAQLGAH"
BASE COUNT          633 a          669 c          751 g          623 t
ORIGIN      
        1 cggtgatgtc gaactcctgt ttgtattcca aagtgccgta gatagcgttg ttgacgtagt
       61 tgggagatac ggggacacga ccaagttcgg tgacgccgcc aatcaccagg gccggtgatg
      121 tgccgatggg ttggtcgaac aaaatcagcg cgatgtcgta atcgggtctg aatcccaact
      181 gcgtgatggt ttgcgagagc gggctttgat ccccgctggc ataaccgctc tgaatgtgta
      241 cctcgccacc aaaaggccat tctgcgtcaa attccattgc ccccatgatg atttgcaggt
      301 cgttttgtgt cccattgagt ggaagttcta tgggggacgt gaggggattt gtgatgccag
      361 cttgtgaatc ggcatcgagc gcgatggcgt caatggcgat cccgggggcg agcttgccgc
      421 ccatataaac gccttcgagg gccaaggtgt aatcacgcgc gaaatgaaaa cgtgcgtaca
      481 catcgcccat gtacaccagc gtgtcaccat cgacacccgc tggcaggtca atcaggtcat
      541 ccacctgacc atcgttatcg acatcgtacg cgggatcggt ggtgtcggcg gggtcaggac
      601 agacataatc acccgggcga ccgtcatcgg cacagttgtc cacgtaggcg gccgtggtgg
      661 tgtaaccgcc ttcattgccg tctctaaagc gggcccctcc gaaaatgcca aactccacgt
      721 gatcggacga aaacatgatg atggcaccgc cctgcatcac atcgttccag ttggaagaca
      781 cgcccgaggt gagtccgctg agagaggggt cggtggtggc ctcgtacgcg aagtcatagg
      841 ccatgccaaa attaagtcgt gacccattgt tgaaattaag tcccgccatg tacatgatgc
      901 gatcgtatgt gtcgccaaag tcaccgtcga agctgtcgcc gtcattgttg agaatcccca
      961 gaccaaaatg agagggctgt cggccaatgc ggaacaaacc agcgggcgtg aggatgtcgg
     1021 cccagatgcg gcggacgttc acattgccgc cgccattacc caaaatatct cccccgatgg
     1081 ggccaatcac gccgtaaggc ccgttcgcgg gactcatgtt tacagtgccc acaacgggat
     1141 tgaagatgtg cagtgtggtg acgtccgatt ggcccatgag caggttgtcc agaaaatcga
     1201 tctgaccgta gagggagatg ttgtcgttga ttttaagttt gggattgagg cgaaaacgtt
     1261 gttgcgcaaa ggcgatggtt ccgaatcgat cgttgctggc gttgttggca tctccgggga
     1321 cgatgcctgc gttgggtcgc tgcaggtcaa ggtcgtgtga atattcaaaa cgcgtgcggt
     1381 aatagccgtg agccgaaaat tcgaatgctt gcgtgttcaa gggcggcaaa cacagcataa
     1441 ccaacaaaca caggacggaa atgaattttg ataatttcat atcgtttagg gattttagtt
     1501 gtcctcccct ataactatag agtataaagg aaatcaactg tttataaggg taaatcaaat
     1561 tcagtacctg aatttttatt atgatgcctg acacatcatt ggacgcgaac gtcagaaagt
     1621 tgcctatgaa taaaatcacg atcacaccgc tgggaggact cggtcgaacg ggagccctta
     1681 actgcatgct ctacgagact gccaagacgg ccatactggt cgattgcggt gccggttttg
     1741 tagacgacgg ttatcccgga gtggacatcc tgattccgaa ttttgatttg ttggaaacca
     1801 aacgcgacaa actcaaggcg gtgatcctca cgcacggcca cgaggatcac gtagggggct
     1861 tgccgtattt gctacaggaa atctgtgtgc ctgtatatgc cacccccttt acgcgaggcg
     1921 tcatcacctc gaaaatttcc gaattcaaaa tcaagggcgt gcaattgcac gagttgcaac
     1981 cctcccacaa gattcagatt ggcgatttcg cgatcgaacc tgtgttcatt catcacagca
     2041 ttatggacgt ggtggccttt tacatcacag tagcagatca aaaaatcttt cattgcacgg
     2101 attttaaact cgatgattcg gcacccgcgc aaaaacagat ggatctggac cgttttgcgg
     2161 cgatcggcaa agcgggagtg gatctggctt tgctggattc caccaatgtt ctggcggaag
     2221 ggacgagtga gagcgaggcc gtgatcaagg acaaccttta cgaaatcttc agttcggtcc
     2281 cgggccggtt gattgcgtgt ttgttctcgt ccaatacgta ccgcctgcaa agtctgttgc
     2341 aatgtgcgcg gcaggcaggg cgcaaggtgg ccctgacggg tcgttccacc aaggaatatt
     2401 ttcgcattgc cgcgtcattg gaccggctcg atctggccgg cattgatttt cacgatgttg
     2461 aggacatcgg tcaatttgcg gaccaggagg tgttcgtgat tgtgactggt tcgcaggccg
     2521 aaccccggtc cgtactgagt cgcatgtcac gcgacatgtt tcgccccttc aaaattcggg
     2581 agggagacac tctgctcatg agttcacgca tgatacccgg caatgaaggc cgtatcttac
     2641 acatgctcaa caacatcgct caactggggg cgcaca
//
LOCUS       DATDUN010000350         1230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_142651, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000350 DATDUN010000000
VERSION     DATDUN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142651"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..695
                     /locus_tag="VJC18_04885"
     CDS             228..695
                     /locus_tag="VJC18_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HLD44749.1"
                     /translation="MHPKRYLYDIGILYDLRLKGLTEISLDDLGSNVLRTPLGGLIEN
                     AVALSLNQQFPDDVFGIRLSHQTEIDFGIKHQGVVFPIECKMALKFKQNYLKGLSIFQ
                     QKIAKKTHSLLLYGGPKYLYSEHIHALPYYLCDGMKSYLEKLTSLKSDVFSLE"
     gene            741..>1230
                     /locus_tag="VJC18_04890"
     CDS             741..>1230
                     /locus_tag="VJC18_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HLD44750.1"
                     /translation="MTIVWLFISLVLLLGLNAFFVLAEFAIVKVRPSRVEELIDSGNK
                     TAKILAQIQTNLDEYLSVCQVGITLASIALGMVGKNTIDVLMHNQQYSLFRYALAMTV
                     SFVLVSGSHIVLGEQVPKYIAIRIADKAALWCTRPLRFFRHLFYPFLGLLTWLANAIL
                     RML"
BASE COUNT          306 a          289 c          278 g          357 t
ORIGIN      
        1 atagattata gaatagatat tatcaagtta tttcgtttga atacctataa taaagcgctt
       61 tgagagaata gatattgtac tggatactcc accttttggc ctaccgggtg ggggcaggca
      121 gggctcccgt cacgggatga tctggaagat ggcgttcgca tcatttatcc cgcggcgttg
      181 gccatgaatt acgggatcgc ggcgggggtg aaccagaaca aaataaaatt catcccaaac
      241 gttaccttta cgatattggt atcttgtacg atctacgcct gaaaggactg acggaaatct
      301 ccttggatga tctcggttca aatgtgttga gaacaccact gggtgggctc attgaaaatg
      361 ctgtggccct ttctctcaac cagcaatttc cggatgacgt ctttggcatc cgcctcagtc
      421 accagactga aatcgatttt ggcatcaaac atcagggtgt cgttttcccc attgaatgca
      481 agatggcttt aaaattcaaa cagaattatc tgaaagggct ttccatcttc cagcagaaaa
      541 tcgcaaaaaa aactcattca ttgcttttgt acggcggacc caaatattta tactcagaac
      601 acatccatgc gttgccctat tacctatgcg acggaatgaa atcatatttg gaaaaactga
      661 catcactcaa atctgatgtt ttttcccttg aatagcgaca ggttaacaat taggtctgtt
      721 tccctctggg gggatcactt gtgacgattg tgtggctttt catttcgctt gtcctgctct
      781 tgggtctcaa cgcgtttttc gtgctggctg aattcgccat cgtcaaggtc cgtccatcgc
      841 gtgttgagga attgatcgac tctggcaata agacagccaa aattctcgct caaatccaaa
      901 ccaatctcga tgagtacctg agcgtgtgtc aggtgggcat caccttggcc agcattgcct
      961 tgggcatggt cggcaaaaac accattgatg tgctcatgca taatcagcaa tacagtctat
     1021 ttcgctatgc gctggccatg actgtctctt ttgtgctcgt atcgggttct cacatcgttt
     1081 tgggtgaaca ggtccccaaa tacattgcca tcagaattgc ggacaaggcc gctttgtggt
     1141 gcacacgacc cttgcggttt ttccgtcatt tattttaccc atttttggga cttctgacct
     1201 ggctcgcaaa cgccatcctc agaatgctgg
//
LOCUS       DATDUN010000351         3604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_267184, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000351 DATDUN010000000
VERSION     DATDUN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267184"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VJC18_04895"
     CDS             complement(<1..650)
                     /locus_tag="VJC18_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44751.1"
                     /translation="MPITRTQTEIDALKSTTQALDTNKDGQVDKPEYYQGHHDIEPAI
                     TNFARGEFHIVTFDDYKYWTQSYSAGTPAKFWVTKKTDVFATDDGFDMQAEIRSAYDD
                     GQILDMALAIENDPRHDEAMVKLAKLEQDWLAYETDSKQKREKYTDTQLITRICSKCP
                     RDLWQKANLDPRQYFTSLGLSHDQLQTLNVLVDLINFIQTEKGTQYVPDASTTDLL"
     gene            1111..1551
                     /gene="mraZ"
                     /locus_tag="VJC18_04900"
     CDS             1111..1551
                     /gene="mraZ"
                     /locus_tag="VJC18_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HLD44752.1"
                     /translation="MFRGRYIHTIDDKGRVSIPSKFRELLSSQFDERLIITNFDGCLW
                     GYTYPEWQKIEEKVAALPQFKPEVKALQRVFISAATECVIDKPGRIMIPQTLREYAGI
                     TKELVFVGMTKRIEIWASDRWAKVFEESQNSINSLDSKLADLGL"
     gene            1566..1928
                     /locus_tag="VJC18_04905"
     CDS             1566..1928
                     /locus_tag="VJC18_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLD44753.1"
                     /translation="MEITKMHDISVLHLFGEVSFLEMDHIEKILASLKKTNNNKVLID
                     MSSVDHVHYAVIKKLVENVMHFRRNQGDIKLVSSNSNTKDIIKFTGADQYLEDYASIS
                     EAMLSFLGHAEQNEALYQ"
     gene            1951..2832
                     /gene="rsmH"
                     /locus_tag="VJC18_04910"
     CDS             1951..2832
                     /gene="rsmH"
                     /locus_tag="VJC18_04910"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HLD44754.1"
                     /translation="MNGHESVLLKTTIDSVVTRTDGLYLDCTGGWGGHSIEILSRLTP
                     GGKLWICDYHVETVKGLANRIAHDERATVICSRFSQVFDNLNFSFDGIIADLGISSVQ
                     LADPNLGIGFQLDNAPLDMRLDHRLTTSAADILSTHDASELADIFFYWGGERASRKIA
                     SAIVFDRNQGKHYTHTKQLKELCERVLGRYYHKKKIHPATKIFQALRIAVNHEIDELK
                     TLLETAPHKLSPGGKLAVISFHSGEDGLVKKMFKDLAQTEDFQLAHKKAIKPDDTEIQ
                     RNPRARSARLRVLTRTP"
     gene            2834..3205
                     /locus_tag="VJC18_04915"
     CDS             2834..3205
                     /locus_tag="VJC18_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HLD44755.1"
                     /translation="MFMRHYHTEPRAVQTQKPVTKLDFLKTRIVMKLLVLGIFVVCLS
                     LFYIWSHTRIVQLGYEINELTSAQIDLLNDHKNYQIELVLMKAPQRLQTVATEKLGMA
                     PPQAQNLIAIDGVVTAPVETE"
     gene            3206..>3604
                     /locus_tag="VJC18_04920"
     CDS             3206..>3604
                     /locus_tag="VJC18_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44756.1"
                     /translation="MVTETENNKTPRFRIFIVSLFFCFFYTVIIFRAYMLQIHGNEKL
                     SNLATSQHNANLRVSPQRGTIFDRKGEILAIDVPVASVGIHPHQIQDKDQVIKLLAEH
                     TSLNLKQLAEKLASTKKFEWIERRIPIDNGR"
BASE COUNT         1034 a          850 c          791 g          929 t
ORIGIN      
        1 atcagcaagt cggttgttga tgcgtccgga acatattgtg ttcccttttc tgtttgaata
       61 aaattgatta aatcaaccag cacattgagt gtttgtagct ggtcgtgact gagaccaagg
      121 ctggtaaaat attgtctggg atccagattt gctttttgcc aaagatcccg cgggcacttg
      181 ctacaaatcc gggttataag ttgtgtgtct gtatattttt cccgcttttg ttttgaatcc
      241 gtttcatagg ccagccaatc ctgttccagc tttgccaatt ttaccattgc ttcatcgtgg
      301 cgcgggtcgt tttctatggc caaggccata tcgagtatct gtccatcgtc gtaggcagag
      361 cgaatttcgg cctgcatgtc aaaaccatca tcagtggcaa aaacatccgt tttttttgtt
      421 acccaaaact tggcgggggt ccctgccgaa tagctttggg tccaatattt gtaatcatca
      481 aaggtgacga tatggaactc gccacgggca aagttggtga tggcaggttc tatatcatga
      541 tgcccctgat aatattctgg tttgtcaacc tgaccgtctt tgtttgtgtc taaagcttgt
      601 gtggtcgact ttaaggcatc aatttctgtt tgtgtgcgag tgatgggcat atcttctatt
      661 gtgccagaaa ctttctaaag cgactagtga aaagggggga aagctggggc acggcatcct
      721 ccttttctgg cggacaggtg ccgtgttcgt tcggctctta tcccaaatca acacatcagc
      781 gctcattgtg taaaaacctg ttgaaaaaca atattatgtc gctaattatg tctatttttc
      841 aatcagttat gctaataata ttattttgtg cgatattaat gatattaacc cattgtttta
      901 tataataaac atacgccttg tacacagttt tttttaacaa cacatttcct gccctacaac
      961 ctacaaagcc atcataaatt caatgagtta aattatttca gaaaaaaata ataatgactt
     1021 gactgtcagg aaaaaaatgg gataaaatac cataatgtgg gataaagtgg taaatccaac
     1081 gtagttttaa ataaaaagaa ggtataatca atgtttaggg ggcgctacat tcatacgatc
     1141 gacgacaagg gaagggtctc cattccttcc aagtttcgtg aactgctaag ttctcagttc
     1201 gacgagcgcc tcatcatcac aaatttcgat ggttgcctct ggggttacac ctacccggag
     1261 tggcaaaaaa tcgaagaaaa ggtcgccgcc ctgccccaat ttaaacccga agtgaaggct
     1321 ttgcaacggg tttttatttc ggccgccaca gaatgtgtca tcgacaaacc aggccgcatc
     1381 atgatccccc aaacgcttcg cgaatacgcc gggatcacca aagagttggt ttttgtgggc
     1441 atgaccaagc gcattgaaat ttgggccagc gaccgttggg caaaggtttt tgaagagagt
     1501 cagaacagca tcaacagcct ggacagtaaa ttggccgatc tggggttgta atcacaggag
     1561 ccttcatgga aatcacgaaa atgcacgata tttctgtcct ccacttattt ggagaagttt
     1621 cctttctcga aatggaccac atagaaaaaa ttttggcctc tctcaaaaaa acaaacaaca
     1681 acaaggttct catcgacatg tcttcggtgg atcatgtgca ttacgctgtc atcaaaaaac
     1741 tggttgagaa cgtgatgcat tttcgccgaa accaaggcga catcaaactg gtcagttcca
     1801 actccaacac caaagacatc atcaaattta caggtgcaga tcagtatttg gaagactacg
     1861 cgagtatctc cgaagccatg ttgagttttt tggggcacgc ggaacaaaac gaagctcttt
     1921 atcaatagaa taatcagaat ttacaaaaca atgaatggcc atgaatcagt cctgctcaaa
     1981 acgaccatcg acagtgttgt tacccgaacc gatggtctct acttggactg cacaggcggc
     2041 tggggcggcc attcaatcga aatcctctcg cgactcacac cgggaggaaa actatggatt
     2101 tgtgattatc acgtggagac cgtcaaggga ttagcaaacc gtattgcaca cgatgagaga
     2161 gcaactgtaa tctgttctcg gttttcccag gtctttgaca atttgaattt ttcctttgat
     2221 ggcatcatag cggacctcgg catttccagc gtgcaactgg ctgatccaaa cttgggtatc
     2281 ggttttcagc tcgacaacgc ccccctggat atgcgtctcg atcatcgttt gaccacctca
     2341 gcggcggata tcctcagtac tcatgacgcg tctgaactgg ctgatatttt cttttactgg
     2401 ggtggggaaa gggccagtcg caagatcgcc agcgccatcg tcttcgacag aaaccagggg
     2461 aaacactaca cccacaccaa acaactcaag gaactctgtg aacgtgtttt gggacggtac
     2521 taccacaaga aaaaaattca ccctgccaca aaaatttttc aggcgctccg gatcgctgtc
     2581 aatcatgaaa ttgatgaatt gaaaacattg ctcgaaacag ccccacacaa actttcaccg
     2641 ggcggcaaac tggccgtgat ctcctttcac tctggagagg acgggttggt caaaaaaatg
     2701 tttaaggatt tggcacaaac tgaggacttt caactggcac acaaaaaagc catcaagcct
     2761 gatgacacgg aaatccaacg caacccacgt gcccgaagcg cgaggttgcg cgtgttgacg
     2821 aggacacctt aacatgttca tgcgacatta ccacaccgaa ccacgtgccg tacaaacaca
     2881 gaaacccgtt acgaaactgg actttctaaa aaccaggatc gtgatgaaac tcctcgtgtt
     2941 ggggatattc gttgtgtgtc tgtccctctt ctacatctgg tcacacacca ggatcgtgca
     3001 gttgggctac gagatcaatg aattgaccag cgcacaaatc gatctgctca atgaccacaa
     3061 aaactaccag attgagcttg tgctcatgaa ggcaccacaa agattacaaa ctgtcgcaac
     3121 agaaaaattg ggcatggccc cgccacaggc tcaaaacctg attgccatcg atggggtggt
     3181 cacggcccct gtggaaacag aataaatggt tacagaaacc gaaaataata aaacccctcg
     3241 attcagaatc tttatcgtca gtctgttctt ttgtttcttt tacaccgtca tcatcttcag
     3301 ggcgtacatg ctccaaatcc acgggaatga aaaattaagc aacctggcca catcacagca
     3361 caacgcaaac ctgagggtca gtccgcagag aggaacaatt ttcgatcgca aaggcgaaat
     3421 tctggctatc gatgttcctg tggccagcgt gggtatccac ccccaccaga ttcaggacaa
     3481 agatcaggtc atcaagctgt tggccgaaca cacatcactc aacctgaaac agctggccga
     3541 aaaattggca tcaacaaaaa aatttgaatg gatcgaacgg cgcatcccca tcgacaacgg
     3601 ccgc
//
LOCUS       DATDUN010000352         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_142732, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000352 DATDUN010000000
VERSION     DATDUN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142732"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VJC18_04925"
     CDS             complement(<1..852)
                     /locus_tag="VJC18_04925"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLD44757.1"
                     /translation="MAANFPKLIITTGPEEGKEFDLSTGESFLVGRSDENDIILDDSS
                     LSRRHASIHQKEGVWFIRDEGSRNGTIVNSTALTPAMTTPLTHLDVIRLGIYEMRFAT
                     QSFTNDEVKNFKPAEKAVTPPTLPTNEIATNKNSKNQSEPQTDLLDNPLTTDHQELKE
                     DQKQLLPENHLEEKQKTGKSLLVFTLIALFFCVIGLVIFFSYHRSQNTSQDQDPGAQE
                     TSETEEVTQEAPPSDQLPPPEEAQTKPDTPPADTNAVQQNISEALTTVPKTQGPLQTA
                     AGKQSENL"
     gene            981..2027
                     /locus_tag="VJC18_04930"
     CDS             981..2027
                     /locus_tag="VJC18_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44758.1"
                     /translation="MFVGLTLPAWAQSCQTVASQEAMSNSFLQYLEDQVQVKKDKIVI
                     LPFFDNNLSQQDSVMYHGIPFLIYEMFSSKNPNVVHPYLSFAAINSLAVVGEKLIDPE
                     VIKQVAKTMNAHFVVFGAYQYSENKTMRVMINLYDAKKTEFLDTALSFDTDVNDAFFD
                     LLRATVFDAFKKLSSHFALRSDDYQAPSLQAFRFYAQGLPLAMRYDQANLELAAVYFE
                     KALKENFQKYDDAALALARAHFMMALIQKLNGGDFTLHWTQAKQALGFITQKFSKQPV
                     KYTLTYRYIEAQNLAAQALTAFVVKNFKRAHLHALSGLELLPEDGLLQNIFMTTLEKG
                     EPKKNIVINNPMCF"
BASE COUNT          516 a          480 c          578 g          650 t
ORIGIN      
        1 caggttttcg gattgtttgc cggcggcggt ttgcagtggt ccctgcgttt tgggcacggt
       61 cgttaatgct tcggatatat tttgttgcac cgcgtttgtg tctgcaggcg gcgtgtctgg
      121 ctttgtctgt gcttcctccg gtggaggaag ctgatcagag ggaggggcct cttgagtgac
      181 ctcttcggtt tcggaggttt cttgggctcc gggatcttga tcttgtgagg tgttttgaga
      241 acggtgatac gagaaaaaaa tgaccagtcc gatcacgcaa aagaaaaggg cgatgagcgt
      301 gaacaccagc aggcttttgc ccgttttttg tttttcttcc agatgatttt cgggaagcaa
      361 ttgcttctga tcttccttaa gctcttgatg gtctgtggtc agagggttgt cgagcaagtc
      421 tgtttgaggt tcgctttgat ttttgctgtt tttgttagtg gctatttcgt ttgttggcaa
      481 ggttggtgga gtgaccgctt tttctgctgg cttaaaattt ttgacttcat catttgtaaa
      541 actttgtgtg gcaaaccgca tttcgtagat gcccagacgg atcacatcca gatgagtgag
      601 gggcgtggtc atggctgggg tgagcgcggt gctgttcacg atggtgccat tgcgagagcc
      661 ttcgtcgcgt atgaaccaga caccctcttt ctggtggatc gaggcgtgtc gacgtgacag
      721 gctggaatca tccagaatga tgtcgttttc atccgaacgc cccaccaaaa aagactcacc
      781 ggtgcttaag tcaaattcct tgccttcctc tggacctgtt gtgatgatca gttttgggaa
      841 attagcagcc ataatggttg tgttctaact atgagaaatc acaaagcaaa tttcaaaatt
      901 gtgattgacc acgtcaacgt taaacagtta aatacaaatt cgtgatccgt caaattataa
      961 atgtcattat tccgtgcgtt ttgtttgttg gccttacctt acccgcttgg gcccagtcct
     1021 gtcaaacagt agccagtcag gaggccatga gcaacagttt tttgcagtat cttgaggatc
     1081 aggtacaggt aaaaaaggat aagattgtca tcttgccctt tttcgacaat aatttatcac
     1141 agcaagactc tgtcatgtat cacgggatcc ctttcttaat ttacgaaatg ttttcttcga
     1201 aaaacccgaa cgtggtgcac ccttatttga gttttgccgc catcaattca ttggccgtgg
     1261 ttggcgagaa attgatcgac ccggaggtca tcaaacaagt ggccaaaacc atgaacgcac
     1321 atttcgttgt ttttggggcg tatcaatatt cagaaaacaa aaccatgcgt gtgatgatca
     1381 atctgtacga tgccaagaaa acagaatttt tggacactgc cttgtccttt gataccgatg
     1441 tgaatgatgc cttttttgat ttgctcaggg cgactgtttt cgatgcgttc aaaaaactgt
     1501 ccagccattt tgccttgcgc tctgatgact accaggcgcc cagcctgcag gcatttcgtt
     1561 tttatgctca aggcctgcct ctggccatgc gttacgacca agccaatctg gagttggccg
     1621 cggtctattt cgaaaaggcg ctgaaggaaa attttcaaaa atacgatgac gcggcgctgg
     1681 cgttggctcg ggcccatttc atgatggcac tcatacaaaa gctgaatggg ggtgatttta
     1741 cattgcactg gacgcaggcc aaacaggcct tggggttcat cacgcaaaaa ttcagcaaac
     1801 agcccgtgaa gtacaccctg acataccgat acatcgaggc gcagaatctg gccgcgcagg
     1861 ccttgacggc gtttgtggtc aaaaatttca agagggctca tctgcatgcc ctctcgggcc
     1921 tagaattatt gcccgaagat gggttgttgc agaatatttt tatgacaact ctcgagaagg
     1981 gtgagccgaa aaagaatata gtgatcaata accccatgtg tttttgaggg cgctttcctg
     2041 ctcatgtcgt caggcaacgc tctagccgct atgaaaaaag tgacaatcat tgttgttttg
     2101 atgttgggtg ttttttttgt gtcgagtgcc gatgcggcca aggccaggaa gccttctgag
     2161 cgttccgtga tgcataacaa tctgggtgtc acggcgctgt atcaggaggg cgatatcgac
     2221 aagg
//
LOCUS       DATDUN010000353         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_391529, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000353 DATDUN010000000
VERSION     DATDUN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391529"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VJC18_04935"
     CDS             complement(<1..408)
                     /locus_tag="VJC18_04935"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HLD44759.1"
                     /translation="MKICIIGTGYVGLVTGACFAQTGNTVYCVDNDQTKIKLLNEGFI
                     PIFEPGLEDYVRDNMKKRRLFFTTDLAEGINQADVSFIAVGTPQDHDGSADLKYVRQV
                     CEDICQVATKEVIIATKSTVPVGTGDELDAIFKQ"
     gene            complement(464..823)
                     /locus_tag="VJC18_04940"
     CDS             complement(464..823)
                     /locus_tag="VJC18_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLD44760.1"
                     /translation="MEQETGRNIADRLIDFAVGVIDLSEVLPSSFLGNHIGCQITRSA
                     TSAGANYEEARGAESKADFIHKLGVALKELKESRYWLKLISKTKICKTDTKIAVLQGE
                     CEELCAIIGKSIITTKR"
     gene            906..>2071
                     /locus_tag="VJC18_04945"
     CDS             906..>2071
                     /locus_tag="VJC18_04945"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HLD44761.1"
                     /translation="MLDTRHMSQTFKKHSPRQELLKIEKKIYAQNQLTIKDSSRVTDK
                     NFKVYYRAYKKAIRHYTKSVTPEVVEADIARSRVVFVGDYHTLDQSQRSFVRILRSFF
                     RHQHKEVIICIEAVQQRHQKHLNEFLSGTSDKDTFIKKIGFKAHWFFDLWENYSIIFD
                     FLRYHKIPVFGIEMNSADKRSLKERDAFMAEQIVARAAEHPNKVVFVLVGDLHLAPPH
                     LPREVNQRADKAKLNLPQVLLYQNSPEIYWQLSQKNIVDHTLIVKTGQGAYCRMHTPP
                     IIVQQSYINWLYHEEGVFDWVDPKASFLLIVEQIAKILQLQLPADYAEVEVYTCGDLG
                     FMRLTSFKKKFSKKELKFIQSQLLGSESYFMPQARIVYIANVSIHHAAEEASHYV"
BASE COUNT          521 a          503 c          475 g          572 t
ORIGIN      
        1 ctgtttgaaa atcgcgtcga gttcgtcgcc agtccccaca ggtaccgtgc tcttggtggc
       61 gatgatgacc tctttggtgg cgacctggca gatgtcttcg cagacctggc ggacgtattt
      121 gagatcggcg gagccatcat gatcttgagg ggtacccacg gcgatgaaag acacatcggc
      181 ttgattgatg ccttcggcca aatcggtggt gaaaaacagc cgacgttttt tcatgttgtc
      241 gcgcacgtag tcttcgagtc ccggttcaaa gatggggatg aagccttcgt taagcagttt
      301 gatcttggtc tggtcgttgt cgacgcaata aaccgtgttg cccgtttggg caaaacacgc
      361 gccagtgacg agaccaacgt agccagtacc gatgatacaa attttcataa gggccctttc
      421 aattatagca atgagcaatc gttgcaattg ttgcaacgag agattatctt tttgtagtta
      481 tgatactttt tccaataatt gcacacagct cctcacactc accttgtagc acggcaatct
      541 tggtatctgt tttgcatatt tttgtcttac taatcaattt tagccaataa cgcgactctt
      601 taagttcttt taatgcaact cctaatttgt gaatgaaatc tgcttttgat tcagcccctc
      661 ttgcttcttc atagttggcc cctgctgagg ttgccgatct ggtgatttga caaccaatgt
      721 ggttgcctag aaaactactg ggtaaaactt cggaaagatc aataacgcca acagcgaaat
      781 ctatgagtcg atcagcgata ttccttcctg tttcttgttc catgttttcc ccaattgcaa
      841 attgcactat tgcttattgc gtgattgcta tcattgaata atgtatttcc tctttccccg
      901 tcaatatgtt agacacacgc cacatgtcgc agactttcaa aaagcatagt ccccgtcagg
      961 agttgctcaa gatcgaaaaa aaaatttacg cccagaatca gttaaccatc aaggattcct
     1021 cccgtgtgac cgacaagaac ttcaaggttt attaccgcgc ctacaaaaag gccattcgtc
     1081 actacaccaa atcagtgacc cctgaggtcg tcgaggccga catcgcgcgt tcccgtgttg
     1141 tctttgtggg ggattatcac acgctcgatc aatcgcagag atcctttgtg cgcattttgc
     1201 gcagtttttt tcgtcaccag cacaaagagg tgattatttg cattgaggcg gtgcaacaga
     1261 ggcatcaaaa acacctcaat gagtttttgt cgggcacgag cgacaaagac acgttcatca
     1321 aaaagattgg attcaaggcg cactggtttt ttgatttgtg ggaaaactac agcatcattt
     1381 ttgatttttt gcgttatcac aaaattcctg tttttggcat cgagatgaac agtgccgaca
     1441 aacgctctct caaagaacgc gacgcgttca tggccgaaca aattgtggcg cgggccgccg
     1501 agcaccccaa caaagtcgta tttgtgctcg tgggcgattt gcatttggca ccgccgcacc
     1561 taccacgcga ggtaaatcag agggctgaca aagcaaagct caatctgcca caggttcttt
     1621 tgtaccagaa tagccccgag atctactggc aattgtccca aaaaaatatt gtcgatcaca
     1681 ccctcattgt caaaacgggg cagggtgcct attgccgcat gcacacgcca ccgatcattg
     1741 tccagcagag ctacatcaac tggctgtacc acgaggaggg cgtgttcgat tgggtcgatc
     1801 ccaaggcgag ttttctgctc attgtggaac agatcgccaa gattttgcaa ctccaactgc
     1861 ctgccgatta cgctgaggtt gaggtctata cctgcggtga tttggggttc atgcggctca
     1921 cgagtttcaa aaagaaattt tccaaaaagg agctcaaatt tattcaatcc cagcttttgg
     1981 gttccgaatc ctatttcatg ccgcaggcac gcatcgttta catcgcgaac gtgtcgattc
     2041 atcacgcggc tgaggaggcg agtcattacg t
//
LOCUS       DATDUN010000354         1083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_391579, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000354 DATDUN010000000
VERSION     DATDUN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391579"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VJC18_04950"
     CDS             <1..258
                     /locus_tag="VJC18_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44762.1"
                     /translation="AAFTAAWISVNGTYTGCDKSGKRGMVLIHVAFLLHGSLFVLPRT
                     TPVFHIPSVKIMQKMTPTLLFSPQMCAACNRSRNRAFWFLG"
     gene            448..>1083
                     /locus_tag="VJC18_04955"
     CDS             448..>1083
                     /locus_tag="VJC18_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44763.1"
                     /translation="MTESAVTISRDLIKKLPKTDLHVHLDGSVTPQTIVELAKKQKID
                     LIDVAKKQGLANLKTGDASELEKKIFKESYQSLAEYLVSFEFINIVLRCYDGLKEASY
                     RLACDNFKEGVRYVEVRFAPQKHWGSDFDWVDIVKAVDDGFKQAAKEYNQKKEIKSGE
                     EPEYKYGIILCAIRMINQNMGKYYRYLYELHVGADLQNLAAMSSYEVAKLVN"
BASE COUNT          302 a          263 c          252 g          266 t
ORIGIN      
        1 gccgcattca ctgccgcatg gatctctgtg aatggcacgt acacgggttg tgacaaaagt
       61 ggtaaacggg gaatggtgct catacacgtt gcctttctgt tacacggttc tctgtttgta
      121 ttaccaagaa caacccccgt atttcacatt ccctctgtaa aaatcatgca aaaaatgacg
      181 cccacactac ttttcagtcc acaaatgtgt gcggcttgta atcggtcacg caatagagcg
      241 ttttggtttt tggggtgata cggggagcta ttattttgac gcggcctgcg gagcaagccc
      301 cgcttgcgaa gagcgtcaaa aaatagcaac ccgaagcagg acccgaaaaa ccaaaacgct
      361 ccgtttactg acggatacgt gtgattttct taaatcgtgg acaattttgc caataatgtc
      421 tataggcctc gccttaaaag gagccttatg actgaatctg ctgtaaccat ttctcgcgat
      481 cttataaaaa aacttcccaa aacggacctg catgtccacc tcgatggttc ggtcacgcca
      541 caaaccattg tggagttggc caaaaaacaa aaaatcgacc tcattgatgt tgccaaaaag
      601 cagggtttgg ccaatctcaa aactggcgat gcctctgagc tggaaaaaaa gattttcaag
      661 gaatcgtatc aatcactggc ggaatacctg gtctcgtttg agttcatcaa catcgtgctt
      721 cgctgttacg acggccttaa ggaggcctct taccggctcg cctgcgataa ctttaaggaa
      781 ggtgtgcgtt acgttgaggt ccgttttgcc ccgcaaaaac actggggaag cgattttgat
      841 tgggtcgaca ttgtcaaggc cgttgacgac gggttcaaac aggccgccaa ggaatacaat
      901 caaaaaaaag aaatcaagtc cggtgaggag cccgaataca aatacggcat catcctgtgc
      961 gccatccgca tgatcaacca gaacatgggc aagtattacc gttatctgta tgaactccat
     1021 gtcggtgccg acctgcaaaa tctggcggcc atgtcctcgt atgaagttgc caaactcgta
     1081 aac
//
LOCUS       DATDUN010000355         2667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_21131, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000355 DATDUN010000000
VERSION     DATDUN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21131"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..>2667)
                     /locus_tag="VJC18_04960"
     CDS             complement(325..>2667)
                     /locus_tag="VJC18_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44764.1"
                     /translation="EWLELSNMDLRPQSNELAINGFVIGTFGLGTGSGRVVSPPIKLL
                     VRGVSAKKDGYQLIEIREDGSFGKTFLWNQSRGEVIEASPSVQAVDANNLSTLLDDAG
                     IPRFDLEPNRFPKKAFNPRGQNHTESTVLDLDPAMRTISDEVLFAGHRPATIRPPRTG
                     DPDWILETMLHLVDQPQSCFLPDVESSFGLTLTGRQQEIFRFVTPQAILEIPMVLFNG
                     KYAVDRKPDPWYGVRGARLMLKDPSHAGMMVQVPVVFHSRRVRQSSRLEMILCLGQNQ
                     FADFDFEFLSCRNTLSRRPLQSLRLVSKNPVTPEIGSREAVFRQQARDDSRILLVEWA
                     HELSLDQNGRYEKIADGNDITLDNGVRFNIGLSDNGEDHIRPTGIMRDENGDFVLGRA
                     VTGQTVRVDLLRSERVGGLLCAVCGTEKKILTVNQLTKSEWFVFNHSRQLDKFFETVT
                     AQRYPMLNRELKEYDVSGRSSAIVDKTYEAEAELSFTVKASLGRYLGASAQSLQTEGS
                     GKPKLSVNLTANKITGQIEVQGLGRMSPDGSGNKERDVEVQIVALPGGVRLYANDAQA
                     GYAWIEIDFKNIEGNWDPVITRARHCFYKFPYDVMPGNAGFLGALWYAAPEDVKICTD
                     LLEEADLSGLRDYLRGSNMRATDLPDVLTRLDQHRFSTTHPRFTLDATALMRASWMGK
                     EPFEKRRRQELESLHPKQDLPDMTTVLGREWRRLSVHRSFVATPTPITIPPKQEQFNG
                     SRCGAGLLSGVKPAALNPFASAKMLTMPMPIMMGGIGLRV"
BASE COUNT          712 a          686 c          595 g          674 t
ORIGIN      
        1 cgaaatcata gcacaagttg aaaaaaaggc ctcgctctac aaactcgcgt caaaacaaag
       61 cctgcagacc gtgttgatca cttcttcacc accaaccaag gcactcatcg aagcccggta
      121 tttcagtcgc attttgcttc tggaagattt ggtttgagct acggttgaga aaagatcgtc
      181 acattcataa ggggcatctc aaaaaacccc cgcagatagc atttgcccca tcgcaaaaag
      241 cagtacgttt ttgacaacct gttttgagtt gggtaaccct gatcgaagag tctgtattta
      301 tcgattggtg cgggctcagt agttctaaac acgcaaaccg atcccaccca tcatgattgg
      361 cattggcatg gtaagcattt ttgccgaggc aaagggattt aatgccgccg gttttactcc
      421 tgacaagagg ccggccccac aacgagaacc attaaactgt tcttgctttg gaggaatggt
      481 aatgggtgtt ggcgttgcaa caaaagatct gtgaaccgac aatcgccgcc attcacgacc
      541 caagacagtg gtcatgtcag gcaaatcttg tttagggtga aggctttcca attcctgacg
      601 acgtcgtttt tcaaatggct cttttcccat ccaggaggca cgcatcaggg ccgttgcatc
      661 taaagtaaac cggggatgtg tggtcgaaaa acgatgttga tcaagtcgtg tcaaaacatc
      721 tggcaaatct gttgcccgca tatttgaacc tcgcaaataa tcgcgaagac ctgataaatc
      781 ggcctcctcc aataaatcgg tgcaaatttt aacatcctct ggcgcagcat accacagggc
      841 tcccaagaaa ccggcgttac ctggcatgac atcgtaaggg aatttgtaaa aacagtgccg
      901 cgcacgtgtg atcacaggat cccagttccc ctcaatgttc ttgaaatcaa tttcgatcca
      961 ggcgtaaccc gcttgagcat cattggcata cagacgcaca ccaccgggca gagctacaat
     1021 ctgcacctcc acatcacgtt ctttattccc acttccgtcg ggggacattc tccccaaccc
     1081 ttggacctcg atttgaccgg tgattttgtt cgctgttaaa ttgacagata gttttggttt
     1141 tcccgagcct tctgtctgta aactttgtgc tgaagcgccc agataacgac ccagagacgc
     1201 cttgaccgtg aacgagagtt ctgcttctgc ttcgtatgtt ttatccacga tggccgatga
     1261 gcgtcccgat acatcgtatt ctttcagctc ccggttcaac atgggatacc gttgtgcagt
     1321 cacggtttca aaaaacttat ctagttgacg cgaatgatta aaaacgaacc actcagattt
     1381 ggtcagctgg tttacagtga ggattttttt ttcggtcccg caaaccgcac agagaagccc
     1441 ccccacacgt tcagaacgca aaaggtcaac acgaacagtc tggcccgtaa cagcacgacc
     1501 caaaacgaaa tccccatttt cgtcgcgcat gattcctgtg ggcctgatgt gatcttcacc
     1561 gttgtcggaa agaccaatgt tgaaacgcac accattgtcc agtgtgatgt cattcccgtc
     1621 agcaattttt tcgtaacgtc cattctgatc aagggagagt tcgtgtgccc attctacgag
     1681 caaaatacga ctatcatctc ttgcttgttg cctgaagact gcttctctgg aaccaatttc
     1741 tggagtgaca gggtttttgg aaacaagtct taaactctgc aggggtctgc ggctcaaggt
     1801 gtttcgacac gaaagaaatt caaaatcaaa atcggcaaac tgattttgac ccaaacataa
     1861 aatcatttcc agccgcgatg attgcctaac ccttcgtgaa tgaaaaacca ccggcacttg
     1921 aaccatcatg ccagcatgtg aagggtcctt aagcattaag cgagcacctc tcacaccgta
     1981 ccaaggatca ggtttgcgat ccaccgcata ctttccattg aaaagaacca tggggatttc
     2041 cagaatcgct tgtggtgtca caaaacgaaa aatctcctgc tggcgcccgg ttagggttag
     2101 gccaaaggag gattccacat cgggtaaaaa acaagactgg ggctggtcaa ccaggtggag
     2161 catggtttca agaatccaat cagggtcacc tgtgcgcggc ggacgtatcg ttgccggtcg
     2221 gtggccagca aacaacactt catcagagat tgttctcatg gcagggtcca aatcaagaac
     2281 tgtagattct gtgtggtttt gaccgcgcgg attgaatgcc tttttgggga acctgtttgg
     2341 ttccaaatca aagcggggga taccggcgtc gtctaacaat gtagaaaggt tgttggcatc
     2401 aacagcctgc acagaaggac tcgcctcaat gacctctccc cgcgattgat tccataaaaa
     2461 agtctttcca aaactcccgt cttccctgat ttcaatcagc tggtagccat ctttttttgc
     2521 agaaacaccc ctgaccaaca atttgatggg tggtgaaaca actctgcccg atcctgtccc
     2581 taaaccaaat gttccgatca cgaaaccatt gatagccagc tcattgctct gtggtctcaa
     2641 atccatattt gacagttcca accactc
//
LOCUS       DATDUN010000356         2002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_433314, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000356 DATDUN010000000
VERSION     DATDUN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433314"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VJC18_04965"
     CDS             <1..1479
                     /locus_tag="VJC18_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HLD44765.1"
                     /translation="KQSENLPIPTDDGAEKAANNDAQTSPNVPTQQDDSFTTTPVSTV
                     TEQPSTALREFTVLLDIKTEPYPATIYFKGERLCTAPCRQQASVNANDTYTLAADYDL
                     RELNDIYRKKVEFTVKPNTDVLELTIKAEIGQLKVSKLPMRIEFYLEGYYDYDQLKAN
                     PAKINDVAYGKSIYLPYGKYVVELREKTQIAGSQNTITQIRFQREYTINQDNPTIDLN
                     VTDRDLQFFPANIKSDPPGAEVFYSDEKMGNTPFSGLLPLGPHQIKLIKEGFFPTIID
                     IDMRMNSVYETTVTLKTSKMGELINEAREQIRNAQWPNALDTLVNALKFGGSIKEKSE
                     VYFLLGSVHNNQKSFDQAVPYLERAKENPDYYTQAALELVRSYHGLTKDIEALKLIVE
                     VLANLRPETSADLRKEANNVFRLISPVKSVIYIYTEPEGAKVFINDKIIEQASPLILS
                     DLGLGNYRIQIEKSGYQTYKTNQNLKIGEFVLIKVRLTPEQL"
     gene            complement(1506..1823)
                     /locus_tag="VJC18_04970"
     CDS             complement(1506..1823)
                     /locus_tag="VJC18_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44766.1"
                     /translation="MITLTGLLLSLGVVAKKTDEVGPHSSLVLVAGQTMRHFDIDKDG
                     VLNRYENSLYNSHKIFGYPLITKPSEALYDINHNLMLEPFEQQTYLKDKKSGTSSRTN
                     NKH"
BASE COUNT          596 a          531 c          414 g          461 t
ORIGIN      
        1 aaacaatccg aaaacctgcc aatcccgacg gacgatgggg cagaaaaagc ggcaaacaat
       61 gacgcgcaga cgtcgcccaa cgtcccaaca caacaagacg attccttcac aacgacacct
      121 gtcagcactg tgaccgaaca accctctacc gcactcaggg aattcactgt actgctcgac
      181 atcaaaaccg aaccttatcc cgccaccatc tactttaagg gcgaacgcct ctgcacggcc
      241 ccctgtcgcc aacaggcttc ggtcaatgca aacgatacct acaccttggc cgcagactac
      301 gacttgcgtg agctcaacga catctatcgc aaaaaagtgg aatttacagt caaacccaat
      361 accgatgttt tggaattgac catcaaagcc gagatcggcc aactcaaggt cagcaagctt
      421 cccatgcgca ttgagttcta cctggaaggg tattacgact atgatcaact caaagccaat
      481 cctgccaaaa tcaacgatgt ggcctacggc aagtccattt acttgcccta tggaaaatat
      541 gtcgtcgagc tgagagaaaa aacccagata gcgggttcac aaaacaccat cacgcaaatc
      601 cggttccaaa gggaatacac catcaaccaa gacaatccca cgattgatct gaatgtcaca
      661 gacagggacc tgcaattttt tccagccaac atcaaatcag accccccggg cgccgaggtc
      721 ttttattcgg acgaaaaaat gggcaacaca ccgttttcag gccttcttcc gttgggacct
      781 catcagatca aactcataaa agaggggttc ttccccacta tcatcgacat cgacatgcgt
      841 atgaattcgg tttatgaaac aaccgttaca ctcaaaacat cgaaaatggg tgagctgatc
      901 aacgaagcca gggaacagat tcgcaacgcg caatggccaa acgcgctcga tacactcgtc
      961 aacgccctca agtttggagg atcaatcaaa gaaaaaagtg aagtgtattt cctactgggg
     1021 tccgtgcaca acaaccaaaa aagtttcgat caagccgttc cctatctgga aagggccaag
     1081 gaaaatccag attattacac tcaagcggcc ctcgagctcg tcaggtcgta tcacggtctc
     1141 acaaaagaca tcgaggccct caaactgatt gttgaagtgc tcgcaaatct taggcccgaa
     1201 acaagtgccg atctacgcaa agaggccaac aatgtcttcc gcctgatctc ccccgtcaaa
     1261 tcagtcatat acatctacac cgagcccgaa ggggccaagg ttttcatcaa cgataaaatc
     1321 atcgagcagg catcaccgct gattttatcg gatctcggct tgggcaacta ccgcattcag
     1381 attgaaaaaa gtggttacca gacttacaaa accaaccaga acctcaagat cggcgaattc
     1441 gtcttgatca aagtcagact cacaccggaa cagctgtgaa gcttagaaat tctttggtgc
     1501 atgattcaat gtttgttatt cgttcgtgag ctcgtcccac tctttttatc cttcaaatat
     1561 gtctgttgtt cgaacggttc cagcatgaga ttgtgattga tgtcgtacag tgcctcactg
     1621 ggttttgtga tcaagggata accaaaaatc ttgtgtgaat tgtagaggga gttttcatat
     1681 cggttcagca caccgtcctt gtcaatgtca aagtgccgca tggtttgccc tgcgaccaac
     1741 accaacgaag aatgcggccc gacttcatcg gtctttttgg caacaacgcc aagtgacagc
     1801 aacagaccag ttaatgtgat cataaaatac atcgcgactg ataccttggc tcgcatgggc
     1861 cagacatggc attttattga aacaagatca atcatcacat tcctctggac ttttgaaaag
     1921 ctcttcttta gggattgcct catgaataaa atcatttcca attttctgat gtgtttgatt
     1981 gtggtcttgt tgctgacagc aa
//
LOCUS       DATDUN010000357         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_556813, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000357 DATDUN010000000
VERSION     DATDUN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556813"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VJC18_04975"
     CDS             complement(<1..401)
                     /locus_tag="VJC18_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44767.1"
                     /translation="MSLRDRLVTAFAVLFATICMYMGTQKLVIHWIKVYKVDLLLPGE
                     DKIPFLPLSIIIYALEYVLPIILFVLITRKKAIKKTVVAYGILIVVQAFFYVVMPVPY
                     VLRPELNINMFGYELVWLLYQVDAPINTFPS"
     gene            complement(541..1032)
                     /locus_tag="VJC18_04980"
     CDS             complement(541..1032)
                     /locus_tag="VJC18_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44768.1"
                     /translation="MIRALFGETYEISLFCFDLIVVIVSHLFLVACGNADAEIDLDGP
                     GTDDSAYPDCDEAGDSMTNGDLVTTTFGNPCLAPAPSESSTEHAIVLDCTARVATAST
                     TDYVKVSCGSGDLDIRFFCSDTSIVKCHGDCGDAEGNDVEMYETTELICDLEDGMFLY
                     GEA"
     gene            complement(1070..2404)
                     /gene="ffh"
                     /locus_tag="VJC18_04985"
     CDS             complement(1070..2404)
                     /gene="ffh"
                     /locus_tag="VJC18_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HLD44769.1"
                     /translation="MFNQLSDKINGVFKKLRNTGVIREANIQDAIRDVRLALLEADVH
                     VSVVKDLLNQIKTRALGQEVIGSNTPGQQFFKIVQSELVKTLGGEQKNLIFNGPPPHI
                     VLLVGLQGSGKTTTAAKLALHFRKKGRRPYLVPADFNRPAAVEQLKTLARQINVPAYD
                     TDIKKDPVKTVRKALDEAGEAFCDIVIVDTAGRLHIDDDMMNELQRMQKKLDDPVVLF
                     VADAMTGQEAVNVAKVFHDKININGVILTKMDGDAKGGAALSVQSVAQCPIYFVGMGE
                     KIDEFEPFHPERLVSRILDRGDIMSLIEKAEDVIDVDQARDLAKKFTKNEFDLEDFRQ
                     QLKQMKKLGSLSSLVKMLPGAKSMTKNVDFDHAEGELQRKEAIINSMTVQERRRPKIL
                     NGSRRSRIAQGSGTSVSEVNRFMKEFDQVKVMMKGFGGGKIAALMQRFGKGF"
     gene            complement(2469..>2847)
                     /locus_tag="VJC18_04990"
     CDS             complement(2469..>2847)
                     /locus_tag="VJC18_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLD44770.1"
                     /translation="IVPKDRAAAITPIVFKASAGAIEQLNVVVVTNLVRALEELKKHS
                     FWLYGATLAPDSIALPKIEPAPRSALVLGSEGKGLRHLVSETCDFKVKIPMAVNFDSL
                     NVAQAGTIFLYYFSNKIGVVDRK"
BASE COUNT          781 a          734 c          564 g          768 t
ORIGIN      
        1 atcgacggaa atgtgttgat cggtgcatca acctgataca agagccacac caactcatat
       61 ccaaacatat ttatattcaa ttctggacgc aacacatagg gcacaggcat taccacataa
      121 aaaaatgcct gaactactat taatatacca taagctacta ctgttttctt aattgccttt
      181 tttcttgtaa tcaagacaaa caatattatt ggtaatacat attctaaagc ataaataata
      241 atacttaatg gtagaaaagg tattttatcc tcacctggta gtaatagatc aactttgtat
      301 accttaatcc agtgaataac gagtttttga gtccccatat acatgcaaat ggttgcaaac
      361 aagacggcaa aggccgtgac taagcggtct cgcaaactca taaaaatcct ctcgtctcta
      421 ttgtatcagt tatccacagg ggcgtctacc ctcaggtgtc gacaattgta actggttgaa
      481 agatgtgaaa aaaatgaaat aggccacaga ggaatcgatt tgcattcaat cgctggtgat
      541 ttaggcttca ccatacaaaa acataccatc ctccaagtcg caaatgagtt cggtggtttc
      601 atacatctcc acgtcgttgc cttccgcgtc tccacaatca ccatgacact ttactatgct
      661 tgtatcgcta caaaaaaaac gaatatcgag atcacccgaa ccacagctaa cctttacata
      721 atctgttgtg ctggctgttg ctacccgtgc tgtacaatcc aaaacgatgg catgttctgt
      781 tgaagactcg gaaggtgctg gggccaagca agggttacca aatgttgtgg ttactaaatc
      841 accatttgtc atagaatctc cggcttcatc acagtcgggg taggccgagt catctgttcc
      901 cggaccatca agatctattt cggcatctgc attgccacac gccactagaa acaaatggga
      961 tacaatcaca acaatcaaat caaagcaaaa cagactgatt tcataagtct ctccaaataa
     1021 ggctctgatc aaaagatttt ttgagtttta cacagcttac aaaatattgt caaaatccct
     1081 taccaaatct ctgcatcaga gccgctattt tgccgccgcc aaaacccttc atcatgactt
     1141 tgacttggtc gaattccttc ataaaccggt tgacctcgga cacagaagtg ccactacctt
     1201 gggctatgcg ggaacggcgc gacccgttga gaattttggg gcgtcgtctc tcctgcaccg
     1261 tcatggaatt gatgatcgct tcttttcttt gaagctctcc ctcggcatga tcaaaatcca
     1321 cattttttgt catactcttg gcaccgggta acatcttaac cagtgaactc aaagacccca
     1381 actttttcat ctgtttcaac tgttgcctga agtcctcgag atcaaactca ttctttgtaa
     1441 attttttggc caaatccctg gcctgatcca catcgatcac atcttcggcc ttttcgatga
     1501 gcgacatgat atcgccccta tccaaaatgc gtgagaccag tcgttctggg tgaaaaggct
     1561 caaactcgtc aattttctct cccatcccga caaaataaat agggcactgc gccaccgatt
     1621 gcaccgaaag cgccgcccca cccttcgcat caccatccat tttggtcaga atcacgccat
     1681 tgatgttgat cttgtcgtga aaaaccttgg ccacgttgac agcctcctga ccagtcatgg
     1741 cgtcggccac aaaaaggaca acaggatcat caagtttttt ttgcattcgc tggagctcat
     1801 tcatcatgtc gtcatcaatg tgcaaacgcc ctgccgtatc aacaatcaca atatcacaaa
     1861 acgcctcccc ggcctcatca agggccttac gaactgtttt gacggggtct tttttgatgt
     1921 cagtatcgta tgcgggcaca ttgatctggc gtgccagtgt tttgagctgt tccacagcgg
     1981 caggtctgtt aaagtccgca ggcaccagat agggtcgtct cccttttttt ctaaaatgca
     2041 gggccaattt ggccgcggtc gtggtcttgc cagaaccctg caaacccacc agcaaaacga
     2101 tgtgcggggg cggaccgtta aaaatcaggt ttttttgttc gccgccaagc gtcttgacca
     2161 attcagattg cacgatcttg aaaaactgtt gacccggcgt gttggaacca atcacctcct
     2221 gacccagcgc gcgtgttttg atctgattca ataggtcctt gaccacactc acgtggacat
     2281 cggcctccag aagggccagg cgcacatcgc ggatggcgtc ctgaatattg gcttcgcgga
     2341 tcacgccagt gttgcgcaat tttttaaaaa caccgttaat tttgtcggat agttgattga
     2401 acattcattc gtgaatcgtt gatcgtggat cgtggatcgt aaatcatttc tgatctgaaa
     2461 taaatcctct attttctatc aacaacccct attttattgc taaaataata caaaaaaatc
     2521 gtccctgcct gcgccacatt gagggaatca aaattcacag ccatcgggat cttgacctta
     2581 aaatcacaag tctcactgac cagatgccgc aagcctttgc cctcagaacc caataccagc
     2641 gcagacctcg gggcgggctc aatctttggc aaggcaattg agtcgggtgc gagtgtcgct
     2701 ccatataacc aaaacgagtg ttttttcaac tcttccagag cgcgcaccag attagtgacc
     2761 acaaccacat tcaattgttc aatggccccg gcactggcct tgaacacaat cggtgtgatt
     2821 gccgccgccc tgtctttggg cacaatc
//
LOCUS       DATDUN010000358         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_143023, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000358 DATDUN010000000
VERSION     DATDUN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143023"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VJC18_04995"
     CDS             <1..1407
                     /locus_tag="VJC18_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44771.1"
                     /translation="APEVLPKVGETAILSVQKKGDTKALQANDDDTLSPLTIGTTTYK
                     YTQESLEALRIYIVGLFQNKPEVYFGDSFASISFSAKEHVESASQTKFNEEITEIDRT
                     ATSDIDRVIAQLNTMLQSHPDRFKSVTITGIASATGVKVAGPNGKKRVITHGNNGTYS
                     IGNIFETDGITLKAGYSFASDLLAKGYTDDQRKYHRGNFDYAKARHDAVAAYLEEKLG
                     AEKYKLIEAKISITEVGGRREPSCKLDIAYVVDTRFKVDESAFDSVGFVKPEPKERLA
                     QFIRTELQAYFEAPTQDKAIAVLRTIAEYFPEKKTAVAEALYTHLEKNVASAPGAEGL
                     MIGLNTFFKLGKTPSVAKTPVILDTADKAPSVITLEPGARLTIPGAEGASLFLGSRDV
                     TNLFNYSAGNYVSKATFSATDIGAMRSAFGGTVTYAVKTDGSTKSVSVTLVGGTVATP
                     RQKTEQPRVVEDPASPDD"
     gene            1452..2558
                     /locus_tag="VJC18_05000"
     CDS             1452..2558
                     /locus_tag="VJC18_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44772.1"
                     /translation="MTTRYVTIGNTTLAKWETEFDSGRKGSDSCVDDTGLDDNSDKNV
                     VAITLGDEVFEKKVGDYYTKKPVADEPSMADIQNLLNAMTCQGATCNKFVLPLQESQK
                     NNFETLFGEAIVSKAPYATNANIYKVELDFGDSVDNTELQALINFRLQMAANQSPQQI
                     ATNLGPDIANLYLYAQEQVTANAEKPQIFSRGVFDGPINMQWINPYMQRTQIDKLFAP
                     GEYANWMSGNYTWFNGNDLTERDFDNIIHINDPTRVSHLTDRDRIANNENDLGILVSY
                     MQTAKEHLKKNAKVNYVNNPVAGVTYANFFYEKDPTTKKGSAVLYYVNAENKWTRRSI
                     RLDDGNQTWFTSSTAEKVTPTEIEQALWKMFVGD"
     gene            complement(2555..2818)
                     /locus_tag="VJC18_05005"
     CDS             complement(2555..2818)
                     /locus_tag="VJC18_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLD44773.1"
                     /translation="MIYSIELTRNAEKGFAQIMKAQPKIGARIASAIDELATAPKLGI
                     PLKGELKGLFKYRVGSYRIIYQIVHSRLIITIIDIGHRKDVYR"
     gene            complement(2815..>3011)
                     /locus_tag="VJC18_05010"
     CDS             complement(2815..>3011)
                     /locus_tag="VJC18_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLD44774.1"
                     /translation="YRYIVSKRGETVAVLLSLEDYESMIETLNETGDREGLRRIKQGM
                     AQAKKGQTVRWQDVKNKMGL"
BASE COUNT          858 a          712 c          715 g          726 t
ORIGIN      
        1 gctcccgaag ttctgccaaa agtgggcgaa acagccatct tatctgtcca gaaaaagggc
       61 gatacaaaag cattgcaggc caatgacgac gacacattat cgccactgac cattggcaca
      121 acaacttata aatataccca ggaaagccta gaagccctgc gtatttatat tgtcgggttg
      181 tttcaaaaca agcctgaggt ttattttggc gattcgtttg cctcaatcag tttttccgcg
      241 aaggagcatg tcgagtctgc cagtcagacc aaattcaacg aagagatcac agagattgac
      301 cgaacagcga cctctgacat tgaccgtgtg attgcacaac tcaacactat gttgcaaagc
      361 catcccgatc gtttcaaatc tgtcacgatc accggcattg cctcggcaac aggagtgaag
      421 gtcgcgggcc caaacggcaa aaagagggtc ataacgcacg gaaataatgg cacgtactcc
      481 attgggaata tttttgagac tgacgggata acactcaaag cgggttactc gtttgcgtcc
      541 gatctgctgg ccaagggtta caccgatgat caaagaaaat atcacagagg taatttcgat
      601 tatgccaaag caaggcacga cgcggtcgcc gcataccttg aggaaaagct cggggctgaa
      661 aaatataagc tcatcgaagc caaaatttcg atcaccgagg ttggcggacg ccgtgaacct
      721 tcatgcaagc tggatatagc ctatgtcgtg gatacgcggt ttaaggtaga cgaatcggcg
      781 ttcgattcgg tgggttttgt caagcctgaa cccaaggaaa ggttggccca atttatccgc
      841 actgagttac aggcttattt tgaagccccc acccaagaca aggccattgc tgtcttgcgc
      901 accattgcag agtactttcc cgaaaagaag actgctgtgg cggaggcctt gtacacccat
      961 ctcgaaaaga acgtcgcttc tgccccgggc gcggaaggtc tcatgatcgg cttgaataca
     1021 ttcttcaagc ttggcaaaac accatcggtt gcaaagactc ctgttatact cgacacagca
     1081 gataaagctc caagcgtgat tacgcttgag cctggcgcga gactcacaat tccaggcgcg
     1141 gaaggggcaa gtctcttcct tgggtcaaga gacgtcacga atctctttaa ttactcggca
     1201 ggtaattatg tgagcaaggc cacattttca gccacagata ttggcgcaat gaggtcagct
     1261 ttcggtggca ccgtcactta tgctgtgaaa acagatggtt ctacaaagtc tgtaagtgtg
     1321 accctcgttg gagggacagt tgctacacct aggcaaaaga cagagcaacc aagagttgtt
     1381 gaagacccag cctctccgga tgattaatgg aagtgcggga cccgaaatga caatagtgaa
     1441 agagggattt tatgacaaca cgttatgtga caattggcaa cacgacactg gccaaatggg
     1501 aaacagaatt tgatagcggt cgcaaaggtt ccgatagctg tgttgacgat accggcttgg
     1561 atgataacag tgataaaaat gttgtcgcta tcacactggg tgatgaagtc tttgaaaaga
     1621 aagtcggtga ttattatact aagaagcctg ttgccgatga gccttcgatg gctgacattc
     1681 aaaacctcct caacgccatg acttgccaag gagcgacttg caacaagttt gtgttgcctc
     1741 ttcaagagag ccaaaaaaat aatttcgaaa ccctttttgg agaagcaatt gtttcaaagg
     1801 caccctatgc cacgaatgcc aatatttaca aggttgaact tgattttggc gattctgttg
     1861 acaacaccga acttcaggcc ttgatcaatt ttaggttgca gatggcagcc aatcaaagtc
     1921 cacagcaaat agccacgaac cttggtcccg atattgccaa tctgtattta tacgcgcagg
     1981 aacaagtgac agccaatgcg gaaaaaccgc aaatcttctc acgcggtgtg ttcgatggcc
     2041 ccattaacat gcaatggatc aacccctaca tgcaacgcac gcagattgac aaactctttg
     2101 cccccggcga atatgcgaat tggatgagtg gcaactatac gtggttcaat ggcaatgacc
     2161 tgactgaaag ggattttgac aacattattc acatcaatga cccgaccaga gtttctcact
     2221 tgaccgatcg cgacaggatt gcgaacaatg aaaatgacct gggcatactg gtgtcttaca
     2281 tgcaaacagc caaagagcac ctcaagaaga acgccaaggt taactatgtc aacaaccctg
     2341 ttgcgggtgt gacgtacgcg aacttttttt acgaaaaaga tcccacaacg aagaaaggtt
     2401 cggcagtgtt gtattatgtc aatgcagaaa ataaatggac caggagatcg ataagattag
     2461 atgacggtaa tcaaacgtgg tttacttcct ccacagcaga aaaagttaca ccaacagaga
     2521 ttgagcaggc gttgtggaag atgtttgtgg gggattaacg atacacatcc ttgcgatgac
     2581 cgatgtcgat gatggtgatg atgagccttg aatgaacaat ttgataaatg atgcgataac
     2641 tccctacccg gtatttaaat aaacccttca actccccctt aagaggaatc cccagtttgg
     2701 gagcggttgc cagttcatcg atcgctgatg caatgcgcgc cccaatcttt ggttgcgcct
     2761 tcatgatctg cgcgaaaccc ttttcggcat ttctggtgag ttcaatactg tagatcatag
     2821 gcccattttg ttcttaacat cctgccaacg gactgtttgc ccttttttgg cctgagccat
     2881 tccttgtttg attctgcgca ggccttcgcg atccccggtt tcgttgagcg tctcaatcat
     2941 gctctcgtaa tcctcgagcg acaacagcac agccacggtc tctccccttt tggacacaat
     3001 gtaacggtag a
//
LOCUS       DATDUN010000359         1056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_516924, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000359 DATDUN010000000
VERSION     DATDUN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516924"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VJC18_05015"
     CDS             complement(<1..507)
                     /locus_tag="VJC18_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44775.1"
                     /translation="MYQLDALSSRADALAAMMIGHFGQGTNRLAKPTHPIDEAVRELS
                     APDWHLGGGMGTTGTNRPTSARYEGSQQMGRRSQLFGSRSHLFGTAGEADDAAGGIVS
                     DDVQAVRRGVIRPENHIIRVIGKIVLNEEQRERLGKLEVRYIASDNPARTDVDEIQSR
                     IGDSTIVLN"
     gene            complement(585..800)
                     /locus_tag="VJC18_05020"
     CDS             complement(585..800)
                     /locus_tag="VJC18_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44776.1"
                     /translation="MCTNENKLQLSDMLVHLRHSLGNHLAVIGSLIAVCKNVAVDDPD
                     FARVVANAEGSYGEILALLQQPGQTVQ"
     gene            complement(803..>1056)
                     /locus_tag="VJC18_05025"
     CDS             complement(803..>1056)
                     /locus_tag="VJC18_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLD44777.1"
                     /translation="QWFAQNSALDETVPREEAVPATRQTSLSCQQINKTLKDYLLEVE
                     KNYIDRALKAHRGNITHTAKVLGISRTTLYHRFHDKEVV"
BASE COUNT          230 a          302 c          229 g          295 t
ORIGIN      
        1 attgagcaca atggtggaat caccgattct ggattgtatt tcgtcaacat cagttctggc
       61 tggattgtca ctggcaatgt atcgtacttc caattttccc aggcgctcgc gttgctcctc
      121 atttaaaaca atcttaccaa tgacgcggat aatatggttt tcgggccgta tcactccacg
      181 tcttacagcc tgcacatcat ccgaaacaat tcccccagcc gcatcatctg cctcaccagc
      241 ggttccaaac aagtggcttc ttgagccaaa caactgtgat ctcctgccca tctgttggct
      301 tccttcataa cgtgctgatg tcggtctgtt tgttccggtc gtccccatgc ctcctcccaa
      361 atgccaatca ggtgctgaca attcccgaac agcttcgtca atagggtgcg ttggtttggc
      421 caacctgttt gtcccctggc cgaaatgacc aatcatcatt gccgccaagg catccgcccg
      481 tgatgataat gcgtcaagct gatacatgtt gtgtcccctc cgtttattgt gcctgactcg
      541 tctcaaagca cactcgattc aaaatattaa tagcgactgt cccgtcattg aacagtttgc
      601 cctggctgtt gtagcagggc cagaatttcg ccataactgc cttcggcatt ggccaccact
      661 ctggcaaaat cggggtcatc gacagcgacg tttttgcaga cagcaatcaa tgacccaatg
      721 accgccaaat gattgcccaa gctgtgtctc aagtggacca acatatcgct caactgtaat
      781 ttgttttcgt tcgtgcacat agtcaaacaa cctctttgtc atgaaagcga tgatacaacg
      841 tggttctgct aatgcccaac accttggccg tgtgcgtgat gttcccccga tgcgccttga
      901 gggcccggtc aatgtagttt ttttcaacct ccagcaaata gtccttgagg gtcttgttga
      961 tctgctgaca cgaaagactt gtttgcctcg ttgctggcac cgcttcttcc cgcggcaccg
     1021 tttcgtccag cgcgctgttt tgcgcaaacc attgcg
//
LOCUS       DATDUN010000360         4465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_516930, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000360 DATDUN010000000
VERSION     DATDUN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516930"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VJC18_05030"
     CDS             <1..1368
                     /locus_tag="VJC18_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44778.1"
                     /translation="LVGVSGADVYSILPESIIKIESLSDNNGVDGIVVDSRDDSIYTS
                     QWQDNSFIRHYDSAFNLIETVIAADYCLNMALDEDNNLLYYTDSVANTISSVNLVTDE
                     VGIVRENIGNGAASNNIGVAVDDDGNLYYSNFYADLEDNKGLFKYNPSLPDGERETKI
                     MDIAYGVGNIYWWSAGEVVIQTNVIVGSLIVMDEGQTEGEPLEHNVNTSALVATSAGV
                     VYLYSADQMLKKVDDGEIQVIGDPLGSGVLTMALDHNDDLFLPIDRFLHRVNLENGMT
                     TQWLAFEDGNMIVRIEYDFKNNEMVVMTESTLAGDEAISIWRTATELGSTPIRVAEIA
                     LEDNDFSAMVVDQETGKVYFYNRLLNELMFVDENDGSVETVLENVIEDDIEAPAGLNF
                     LSSENSLLITYLDRGRYLLPLDGSGPSLFCEMAYGMDNQQGYVDGRGYYYGTNSGVIY
                     RIRPE"
     gene            complement(1446..2270)
                     /locus_tag="VJC18_05035"
     CDS             complement(1446..2270)
                     /locus_tag="VJC18_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44779.1"
                     /translation="MNQEALKYNTDSREGLSFEEIKAMDSELIAQQLRHSSRVGEINR
                     HDIKTFLKSISPDKRKQVQNSLRQNYFLNIKNRLSLFGRHNNGIKYLDRPAGTETTNT
                     REYIQEPATGILIDAYEFAITSASSERFIKTGGVCDCIVLTLYSPSKKTGVMAHVPRR
                     VLPDDPSLATSMQRIMQELSRYGIDANNNDLQARLVGGWPGLSAGLYEQLGQQMKQYG
                     LAVVENSTMVPAVQHLVFDTSNGLLYDVPEITSREPPSLINLPRGALHFFIPDSNF"
     gene            complement(2698..3030)
                     /locus_tag="VJC18_05040"
     CDS             complement(2698..3030)
                     /locus_tag="VJC18_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLD44780.1"
                     /translation="MKIANRFSSVFVMLFLFVGLWGCQRQTALIIDVRSQGEYNTEHI
                     ANAILIPHETIGSEIEKIEENRGREIIIYCRSGHRAGLAKTILEGMGYTHVTNGGGLM
                     DMKAKGYQ"
     gene            complement(3295..3675)
                     /locus_tag="VJC18_05045"
     CDS             complement(3295..3675)
                     /locus_tag="VJC18_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HLD44781.1"
                     /translation="MKICVPTKNNNGLQSEVSDHFGSAPFFMIVDTDTAKCEAIINNN
                     QHHSHGMCQPLQTIGGHDFDSIVVRGIGQGALSKLTSAGKRVLVSSAGTIQSLVDEVK
                     AGQCHDIDPAHSCGHHGHGCGDHG"
     gene            complement(3620..3964)
                     /locus_tag="VJC18_05050"
     CDS             complement(3620..3964)
                     /locus_tag="VJC18_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HLD44782.1"
                     /translation="MRPCKKRRVCCQPYSSYFKPRGVPCSSLDEVVLKPDEIEAIRLA
                     DLEELYQEEAAKKMNISRPTFGRIIDSAHKKIAHALLEGKALRLDVKQKGKIHENLCP
                     HKKQQRPPKRSL"
     gene            complement(4045..>4465)
                     /locus_tag="VJC18_05055"
     CDS             complement(4045..>4465)
                     /locus_tag="VJC18_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLD44783.1"
                     /translation="RTLTVGFLTPFYFLRAGSLVSLPALSAAPLVLVVLLLSKVISKI
                     FGLFPVIGIFHRGTKERWYYTLLMSTGLTFGTISALYGLTHNIVTSEQYSFLVAAVIA
                     SAVVPTMIANFFFLPRHLLKQAPTADEELPELENEDV"
BASE COUNT         1227 a          939 c         1062 g         1237 t
ORIGIN      
        1 ctcgtaggtg tttcgggtgc tgatgtttat agtattctgc ccgagtccat catcaaaatt
       61 gaaagtcttt ctgataacaa tggtgttgat ggtattgtgg tcgattcacg tgatgattcg
      121 atttatacct cacaatggca agataacagc ttcattcgtc attatgacag cgcctttaat
      181 ctcatcgaaa cagtgattgc agctgattat tgtttgaaca tggctctcga tgaagacaat
      241 aatcttttgt attatactga ctctgttgcc aatactattt cgtcagtcaa tttagttact
      301 gacgaagttg gcatagtccg agaaaatatt ggtaatggtg cggcttctaa taatattggt
      361 gttgctgtgg atgatgatgg aaacttgtat tattcaaatt tttatgcgga tcttgaagat
      421 aacaaaggtc tgttcaaata caacccctca ttaccggatg gtgagagaga aacaaaaata
      481 atggacatag cctatggtgt gggcaacatt tattggtgga gcgccggaga ggttgtgata
      541 caaacgaacg tgattgtggg atcgttgatt gttatggatg aggggcagac tgagggtgag
      601 ccgctggaac acaacgtcaa cacttctgcg ttggtggcca catccgcagg ggttgtgtat
      661 ctttattcag cagatcagat gctcaaaaaa gtcgacgatg gcgaaatcca agtcatcggc
      721 gatcctttgg gaagcggtgt tttgacgatg gctctcgatc acaacgatga tctctttctt
      781 cccattgatc gttttcttca tagagttaat cttgagaatg gtatgacgac ccagtggctg
      841 gcttttgagg atggcaatat gattgtcagg atcgagtatg attttaaaaa caacgaaatg
      901 gttgtgatga ctgaatcgac cctggcagga gacgaggcaa tttcaatttg gagaacagcg
      961 accgaactcg ggtcaacccc gatacgggtg gcggaaattg ctctcgagga taatgatttt
     1021 tctgctatgg tcgttgatca ggaaacgggt aaagtctatt tctataatag gcttctcaat
     1081 gagctgatgt ttgttgatga aaatgatgga tcggttgaga cggtgcttga aaatgttatt
     1141 gaagatgaca ttgaagcgcc tgccgggcta aattttttgt cttcggagaa cagtttgctt
     1201 attacctatc tcgacagggg tcgttatcta ctgcctctgg atgggagcgg gccatctctt
     1261 ttttgcgaaa tggcttacgg catggataat caacagggat atgttgatgg gcgtggatat
     1321 tattatggaa ccaattcggg cgttatttac cggatcaggc ctgaatagga tgaaatatag
     1381 aattttgata gctgctgctt gatgtcattc aaaccgccgc cattgccatt tcaatgaatc
     1441 cagttctaaa aattggaatc tggaataaag aaatgcaaag ccccgcgagg aaggttgatc
     1501 aagcttggag gttccctgct ggtgatttca gggacatcgt acaacagacc attagaggta
     1561 tcaaagacaa gatgttgtac cgctggcacc attgtgctat tttcaacaac cgcaaggcca
     1621 tattgtttca tttgttggcc cagctgctca tataacccag cagacaaccc aggccatccc
     1681 ccaacaagac gcgcttgcaa atcgttgttg ttagcatcaa tgccgtaacg agacagttct
     1741 tgcatgatgc gttgcataga tgtcgccaga ctgggatcgt caggtaaaac gcgtcgagga
     1801 acatgggcca tcactcctgt ttttttagaa ggcgaataca atgttaagac aatacaatca
     1861 cacacaccac ccgttttaat aaacctttcc gaactggcag aagtgattgc aaactcatat
     1921 gcatcaataa gaattcctgt agcgggttct tgaatatatt ctctggtgtt tgtggtttca
     1981 gttcctgcag ggcgatccaa atatttaatc ccgttattat gacgcccaaa caatgaaagc
     2041 ctatttttga tgttcaaaaa ataattttgt cttaaactat tttgaacttg ttttctctta
     2101 tcaggagaaa tgcttttcaa aaaagtttta atatcatgcc tgttgatttc tccaactcgt
     2161 gaggaatgtc tgagttgctg agcgatgagt tcgctatcca tggcttttat ttcttcaaaa
     2221 gacagacctt cacgcgaatc cgtattatat tttaaggctt cttgattcac gggttgaaga
     2281 aacttgatcg ccataggttt atgtaatgca aatactgtgc cgcaaacaca aaaaacaaac
     2341 acgcctaagc tcgtgatatg atataagatt atttttacaa gttgaaaggt gcggaacgat
     2401 gttctctatt tgagaaaaat caacatgact aagagcctat ccgaaaacct gtggataact
     2461 gtcgtcccga agggatgagg gaatccagtc gtaggcttta gcctacgggg aaaacaggcg
     2521 ccggaaatat tgcaccctga aagggtgcag gaaaaatgcc acattgtcat ccgccgcccc
     2581 ttcagggcgg aaaaaatatt tcgccacgat ttccgtgggt tgaaacccca ctgctctatt
     2641 ccaccctccc ttcaggaggt tttcggatag actcttaggt ttaaggcagg ccttggctca
     2701 ttgatatccc ttggccttca tgtccataag gccgccgcca ttggtgacat gtgtgtatcc
     2761 catgccttcc aaaatcgttt tggctaaacc agccctgtga ccactacggc agtagataat
     2821 aatctccctg cccctgtttt cctcaatttt ttctatttca ctgcctattg tctcatgggg
     2881 aatcaggatg gcatttgcaa tatgctcggt gttgtattca ccctgtgatc ttacatcgat
     2941 gatcaaggct gtctgtcttt gacagcccca caagcccaca aacaaaaaca acataacaaa
     3001 aacagatgag aatcgatttg ctattttcat ggctcgtttc tggcagattc tgcatcttca
     3061 ttcaatcagg aaatagcatt ttcgggacag atatcaatgc aagcaccaca ggctgtacac
     3121 agcgcagaat taatggtgag aatttccggc ttgcacggga ccgaaaacga ggcacagtat
     3181 ttgatgcatt ttgcgcaact gatgctacac gctgtcgtga tggattggac gggacacacg
     3241 gcaagacata ttttgcacaa tgtacaacgt gccggatcaa tagtgggtgt gtgcttagcc
     3301 atggtcacca cacccgtggc catgatggcc gcaagaatgc gccggatcaa tatcgtggca
     3361 ttggcctgct ttgacttcat caaccaatga ttgaattgtc ccggcgctgg aaacgagcac
     3421 gcgcttgcct gccgacgtga gtttgctcaa agcaccttga ccaatgcctc gaacaacaat
     3481 gctgtcaaag tcatgaccgc caattgtctg cagaggctga cacatgccgt ggctgtggtg
     3541 ctgattgttg ttgatgatgg cctcgcattt ggcggtgtct gtatcgacaa tcataaaaaa
     3601 gggtgctgaa ccaaagtgat cagagacttc gctttggagg ccgttgttgt tttttgtggg
     3661 gacacagatt ttcatggatt tttccttttt gcttgacatc gagcctgagg gccttgccct
     3721 ccaaaagagc gtgagcaatt tttttatggg ctgagtcaat aatgcgacca aacgtgggac
     3781 gcgatatgtt catctttttt gccgcctctt cctgatacaa ttcttccaga tcggccaacc
     3841 gaatggcttc gatctcatcg ggctttaaga caacctcatc gagtgaggaa cagggaacac
     3901 cacggggttt gaaataggaa gaataaggct gacaacaaac acgtcttttt ttacagggcc
     3961 gcatgcgtta tgaacatatg accataatca aaacttggca atgagatttt ttgtttttga
     4021 agtgaaacat aaataaacgt aacatcacac atcctcattc tccaactcgg gcaactcctc
     4081 atccgccgtc ggagcctgtt ttaacaagtg gcgtggcagg aagaaaaaat tggcgatcat
     4141 tgttggaacc acagcgctgg cgatgacggc ggcaaccaga aatgaatact gttctgaggt
     4201 cacaatattg tgtgtcagac catagagagc agaaattgtg ccaaacgtga gtccggtgga
     4261 catgaggagt gtgtaatacc agcgttcctt ggtgccacgg tgaaagatgc caatgacagg
     4321 gaacagacca aatattttag aaatcacttt gctgagcagc agcacaacca agacaagagg
     4381 ggcagcgctc agggcgggca acgaaacaag tgagccggcg cgcagaaaat aaaacggtgt
     4441 gagaaaacca accgtcaacg tccgt
//
LOCUS       DATDUN010000361         1077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_473739, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000361 DATDUN010000000
VERSION     DATDUN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473739"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VJC18_05060"
     CDS             <1..1055
                     /locus_tag="VJC18_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HLD44784.1"
                     /translation="SLVAGTNHVSVMFSHAYGENHLGLLRYQDPLDSKTYVYTAGGSE
                     FARYVFPCFDQSDITSSLNLVLQTPADWRVITQGKADVVSLPNKTNRFWAFRSSQPAV
                     VSSFVLIAGPFEQWQKDHNQLSMRLFVRASLADKIKPVEWFSPIKLGMAFYQNYFKTP
                     YPHNKIDQVFLPEQVSQEGHSALLVMSDASLSSQRRVQVRSLLNKMAHQWLAPNITTA
                     STRDLWLVDGYAQYSAGLAADQIDRLASQQAQALPTNARPEPPVNTTGLDTTLTPQGD
                     DTNFYSYLKTLTPTATRESVSTLASNTAATTCSQERLEQLDRFITNNPRLPGVMKTHF
                     LSAKQQYELCLNKRGE"
BASE COUNT          270 a          310 c          240 g          257 t
ORIGIN      
        1 gaagtttggt ggcaggcacc aatcacgtaa gcgtcatgtt ctctcacgcc tatggtgaaa
       61 accacttggg cttgctgagg tatcaagatc ctctggattc aaaaacctat gtgtacacag
      121 cggggggctc tgaattcgcg cgatacgtct tcccctgttt cgatcaatca gacatcactt
      181 cctcactgaa tctggttttg caaacacctg ccgattggcg agtcatcacc cagggcaaag
      241 cagatgttgt gtccttgcca aataagacga atcgtttctg ggcctttcga agctcccaac
      301 ccgcggttgt cagttctttc gtcctcatcg cggggccctt tgagcagtgg cagaaggatc
      361 ataaccaact ctccatgcgc ctgtttgtca gggcatcttt ggctgacaag atcaagcctg
      421 tggagtggtt ttctcccatc aaactgggaa tggcttttta tcaaaattat tttaagacac
      481 cctatccaca caataaaatc gatcaggttt ttttgcccga acaagtctct caagagggcc
      541 actcagcact gctcgtaatg agcgatgcct ctctgtcctc gcaaagaagg gtccaggtgc
      601 gctcgctact caataaaatg gcacaccaat ggttggctcc caacatcacc accgcatcga
      661 ccagggactt atggcttgtt gacggctacg cgcaatattc agctgggtta gccgcggatc
      721 agattgatcg attggcttca caacaagctc aggctctgcc gacaaatgcc cggccggaac
      781 ctcctgtcaa cacaaccgga ctcgacacca cactcacccc ccagggtgac gacacgaatt
      841 tttattctta ccttaagaca ctcactccca ctgcaacacg tgaatcggtc agcacgctag
      901 ccagcaacac agccgcgacc acctgctcac aggagcgcct tgagcaacta gatcgtttca
      961 tcaccaataa cccgcggctc cccggtgtga tgaaaacaca ttttctttct gccaaacagc
     1021 aatatgagtt gtgtctgaat aagcgaggag agtaacggtc tctgacgcca tcacaca
//
LOCUS       DATDUN010000362         1289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_143169, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000362 DATDUN010000000
VERSION     DATDUN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143169"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1289)
                     /locus_tag="VJC18_05065"
     CDS             complement(<1..>1289)
                     /locus_tag="VJC18_05065"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HLD44785.1"
                     /translation="YYFVKLSSQDAASRAVITEIPFYASGTDYSYWSMDEAHGLELVA
                     DKGSYEFGDTAKVLIKSPYPKTQALIAVERDRIMSYEVKLLEGTSPVLDIPIKKEYAP
                     NVYVSVVLLKGSEEIDPSLVAQKQEGQLTSDPLIKAGKIELNVKPYQKNLDLEVRADR
                     ASYHPGDTVTVSFKLKDFQSPEKAEITVMAVDEGVLLAGGYQLANPLDTLYEYYEHGV
                     ALMDSRTRFVGKQGAKEKLEEPSSGGGQMDGYRQKFIPLAYYNDAVETDENGQATVSF
                     VLPDQLTSFKVMAIANAQVDQFGLGETTFKTSKEIMIRPALPRFIRDGDHFESDVAIH
                     NQTNEDLEVTLSVDAVGLEVTLGAVGKITVPAQKTAVYPVAFTVAPGLFEGGPTKTVR
                     VAAEKTAQAKVVFDAKTKNQSDQVIIKLPVYQERLVE"
BASE COUNT          292 a          364 c          302 g          331 t
ORIGIN      
        1 tttccacaag ccgttcctga tacaccggga gtttgatgat cacctgatcg gattgatttt
       61 tggttttggc atcaaacacg actttggcct gagctgtttt ctcggccgca accctaaccg
      121 tctttgtggg accgccctca aacagaccgg gtgccacagt aaacgccacg ggataaaccg
      181 ctgttttttg ggctggcaca gtgattttgc cgacagcacc caaagtcacc tcaagcccaa
      241 cggcatcaac actcagcgtc acctccaggt cttcattggt ctgattgtga atcgccacat
      301 ccgattcaaa atgatctccg tccctgatga accgcgggag cgccggcctg atcatgattt
      361 ccttgctggt cttgaaggtc gtttcaccca acccaaactg gtcaacttgg gcgttggcga
      421 tcgccatcac cttgaatgag gtcagttgat ccggcaaaac aaaagacacc gtggcctgcc
      481 cattctcatc tgtctccacg gcatcgttat agtacgcgag cggaatgaat ttttgtcgat
      541 aaccatccat ttgcccccca ccgctggagg gttcctcgag tttttccttg gcgccttgtt
      601 ttcccacaaa tcgcgtgcga gaatccatga gggccacacc gtgttcataa tattcgtaca
      661 gggtatccag agggttggcc agttgatacc ctcccgccaa caacacaccc tcatcgacag
      721 ccatgaccgt gatttcggcc ttttctggag attggaaatc cttgagcttg aacgaaaccg
      781 taactgtgtc tcccgggtga taagaggcac gatccgctct cacctccaaa tccagatttt
      841 tctggtacgg cttgacattg agttctatct tgcccgcctt gatcagagga tcggacgtga
      901 gttgcccctc ctgtttctgc gccacaagtg atggatcgat ctcttcagaa cccttgagca
      961 gtaccacact cacatacaca ttgggggcgt attctttttt gatgggaata tccaaaacag
     1021 gcgaggtccc ctcaagcagt ttgacctcgt aggacattat tcgatcacgt tctaccgcaa
     1081 tcagggcctg ggttttcgga tagggggatt tgatgagcac cttggcggta tccccaaact
     1141 cataactgcc tttgtcggca accaactcca gtccatgggc ctcatccatt gaccaatacg
     1201 agtaatctgt gcccgatgcg taaaacggaa tttccgtaat gaccgcacgt gacgcggcgt
     1261 cctggctgga gagtttgaca aagtaatac
//
LOCUS       DATDUN010000363         1041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_473898, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000363 DATDUN010000000
VERSION     DATDUN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473898"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1041)
                     /locus_tag="VJC18_05070"
     CDS             complement(<1..>1041)
                     /locus_tag="VJC18_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44786.1"
                     /translation="REKDSIFALVEKTHGFHPSIYKAYRQRGEALFEELAGYVSKILD
                     DDFGLAEMQKIQDEHGEEFLLGVIQMASPTSGASYVKREEHLDLLRKIRTAGDLRAHV
                     PSSWKGKLETFEQELGQWQIRTGENADPLGKVSAMLARLRSRKGQEPVTQDQLVTVLA
                     DYLNAKTPEERSVKHQSVLALLYKLAGQDDALREKIERIHSLDYTALTLLEQLFSDKD
                     LLQKILADALNQVPNDVLASQAKKQPLEGNAHRLIKAIKDLWNKALPTDKKILILGNM
                     LASYHPADIAEKIIPRVDGTLRLAITSAMEAVPVTNKNCILQELLEEPLRLIRTERKK
                     YTYTNAGRITVGL"
BASE COUNT          205 a          256 c          267 g          313 t
ORIGIN      
        1 agtcccacgg tgatgcggcc cgcatttgtg taggtgtatt tcttgcgttc ggtccgaatc
       61 agtctcaggg gttcttccag aagttcctgc aggatgcagt ttttgttggt cacggggacc
      121 gcctccattg ctgatgtgat ggcgagcctc aaggtcccat ccacacgcgg gatgattttt
      181 tccgcgatgt cggctgggtg ataggatgcc agcatattac ccaaaatcaa tatttttttg
      241 tcggtgggca aagctttgtt ccagaggtcc tttattgctt tgatcagtct atgggcgttg
      301 ccttcaagtg gttgtttttt tgcttggctc gccaagacgt cattgggaac ctggttcaag
      361 gcgtcagcaa gaattttctg cagcaaatct ttatcactaa aaagctgttc cagtagcgtg
      421 agggcggtgt aatccaagga atgaatgcgt tcaatttttt cgcgcagggc atcgtcctgg
      481 cccgccagct tgtaaagaag agccagcaca ctttgatgtt ttaccgatct ttcttcgggt
      541 gttttggcat tcagataatc ggcaagcact gtgaccagtt gatcttgtgt gacaggctcc
      601 tgccccttgc gtgacctaag cctggcgagc atggcgctca ctttgccaag gggatctgcg
      661 ttttcaccgg tcctgatttg ccattgtccc aattcctgct caaatgtttc gagtttgcct
      721 ttccaggaag acgggacatg cgctctcaga tcacctgctg tgcgtatttt cctgagcagg
      781 tccaggtgtt cctctctttt tacatagctg gccccgctgg tgggtgaggc catctggatt
      841 acgccgagca gaaactcttc gccgtgttcg tcctgtattt tctgcatttc agccaggcca
      901 aagtcatcat cgagaatctt tgatacataa ccggccagtt cctcaaatag tgcttcaccc
      961 cgttgacgat aggccttgta gatactggga tgaaagccgt gcgttttttc aaccaaggca
     1021 aaaatgctgt ctttttcccg g
//
LOCUS       DATDUN010000364         2146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_102539, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000364 DATDUN010000000
VERSION     DATDUN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102539"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VJC18_05075"
     CDS             complement(<1..871)
                     /locus_tag="VJC18_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44787.1"
                     /translation="MKKSSGGSRDAGIFMQWQKNILSLLTRLYSEFAVRGKRLCACRC
                     DGFRELFFEEMKLPDIRGRHLITAKLRIVIFVVTWIVFFVFFPAVWKIAPVIPVLFNL
                     GFLITSFCYYQMIKNNQPSLSIILLEIFADIVSQTVIIYLLGVKGWAPFLIYGMYIVA
                     LGILSGYYAALISSIMALFCYNFIYFLIISGGLADFAYPQTNIGYIDFDYLRPFLNLF
                     FLPLIFIGIVYSVRIANYFTRVKEQVLERRNIQLTALNHIGATIRRVLNAPKVIDEVL
                     KAVIQGLGFDVCFL"
     gene            complement(846..>2146)
                     /locus_tag="VJC18_05080"
     CDS             complement(846..>2146)
                     /locus_tag="VJC18_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HLD44788.1"
                     /translation="EVYHDQLTLTLKFKLDPQQFRGQNISGVLTFQGCSQRICFRAVK
                     VPFSFDINPTTITRVSNTTDVNQHGKHSLWGLLRARQFEDVMSEGVWIALLLTFIAGM
                     ITGLSPCVLPVIPLTLAFIGVSKEVSTRTKLFHLFIFIIGMVVMYVFLGIMSVTLGMT
                     LGFVFQQRFFLIILIVFLLLMAAWLWGLFSLALPASWQNAIVNCQPRGYLRFFYAGLT
                     IGILATPCVGPLLGPLLVYVAAHQNLSLGMLLMLSYSLGLSALFVVLGFFSNQWIAHF
                     GQKGHILKKVIAVLLLISAFHFALTLIESHAIDEGNDHFFENEFYVAQQKALDQQKDM
                     IVDFYADWCAPCQAWDEEVWQRKEIQERVLKNYVPVKINCTRETKACLEMVNRYRVFG
                     WPTVVFVNNMGIEFPQTRLVGTVMDRDDFMKYLEEIERRQ"
BASE COUNT          690 a          552 c          455 g          449 t
ORIGIN      
        1 ccaggaaaca cacatcgaac cccaacccct gaatgacggc cttgaggact tcatcaatca
       61 ccttgggcgc atttaacact ctgcgaatcg tggcaccaat gtgattgagg gccgtcaact
      121 gaatattgcg tcgctccaac acttgctcct tcacccgggt gaaataattg gcaatgcgca
      181 cagaataaac gatgccgatg aagatcaaag gcagaaaaaa cagatttaaa aaaggacgca
      241 ggtaatcaaa atcgatgtag ccaatatttg tttgagggta cgcgaagtca gccaacccac
      301 ccgaaataat caaaaaataa atgaagttgt aacaaaacag ggccatgatt gatgagatga
      361 gggccgcgta atagcccgat agaatgccca acgccacaat gtacatgcca tagatcagga
      421 aaggagccca acctttgact cccaacaggt aaataatgac cgtctggctc acgatatcgg
      481 caaagatctc cagcaatatg atcgacaagg acggctggtt gtttttgatc atttgatagt
      541 agcaaaatga tgtgatcaaa aaacccagat taaacagcac agggatgact ggggcaatct
      601 tccagaccgc gggaaaaaat acaaaaaaca caatccacgt caccacaaaa atcacaatac
      661 gaagcttggc tgtaatgaga tgccgcccgc gaatatctgg cagtttcatt tcttcgaaga
      721 acagttcacg aaaaccatca cagcgacagg cgcacaaccg ttttccgcga acagcaaact
      781 cagagtaaag tctggtcaat aatgatagta tgttcttctg ccactgcata aaaatcccgg
      841 catccctact gcctccgctc gatttcttca agatacttca taaaatcatc ccggtccata
      901 acggtcccca ccaatctggt ctgcggaaac tcaatgccca tattgttgac aaaaacaacg
      961 gttggccagc caaacacacg gtaacgattg accatttcga gacaggcttt tgtttctctg
     1021 gtacagttga tcttgacagg aacgtagttt ttcaacaccc tctcctggat ttccttacgt
     1081 tgccacacct cttcatccca cgcctgacag ggggcacacc aatcggcgta aaaatcgaca
     1141 atcatgtcct tctgctggtc cagggccttt tgttgtgcca cataaaattc attttcaaaa
     1201 aaatgatcat ttccttcgtc gatggcgtgt gactcaatca gggtcaacgc aaaatgaaaa
     1261 gccgatatca ggagcaacac agcaatcact tttttgagaa tgtgcccctt ttgaccaaaa
     1321 tgggcaatcc actgattcga aaaaaaaccc agcacaacaa acaaagcgct caatcccaat
     1381 gagtaactga gcatgagcaa catgcccagt gatagatttt gatgagcagc cacatagaca
     1441 agcaacggtc ccagcagggg ccccacacaa ggtgtggcca ggatgccaat ggtcagaccc
     1501 gcgtaaaaaa atcgtaaata ccccctgggc tgacagttca caatggcatt ttgccaactt
     1561 gccggcaagg ccaatgaaaa caaaccccac aaccaggccg ccatgagcaa taaaaataca
     1621 atcaaaatga tcaaaaaaaa cctttgttga aacacaaaac ccagcgtcat ccccaaagta
     1681 acagacatga tccccaaaaa aacatacatc accaccatgc caatgatgaa gatgaataag
     1741 tgaaacaatt tggttctggt ggagacctcc ttggaaaccc caatgaaggc cagtgtcaga
     1801 ggaatcactg gcaacacaca gggcgaaagt cctgtaatca ttcccgcaat gaaggtcaaa
     1861 agcagggcga tccacacgcc ctcgctcatg acatcttcaa actggcgagc ccgcaacagc
     1921 ccccaaagag agtgtttgcc gtgttgattg acgtcagttg tgttcgagac ccgtgtgatg
     1981 gtcgtcgggt taatgtcaaa agaaaaggga acctttaccg ccctgaagca aatgcgctgg
     2041 gagcaaccct gaaaggtgag cacgcccgag atgttctgtc ccctaaactg ctgtgggtcc
     2101 agcttgaact tgagggtcag ggtcaactga tcgtgataca cctccg
//
LOCUS       DATDUN010000365         1761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_102559, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000365 DATDUN010000000
VERSION     DATDUN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102559"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VJC18_05085"
     CDS             <1..362
                     /locus_tag="VJC18_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44789.1"
                     /translation="TGRSANKDLASFIVDKAAEIFDLTTLPADFADTLDQIINSPDMP
                     AASEPIEKLFYIQNQLETIYAENGLDLEFSFRPIIALPIASFVRLNVYRSDLGDLEYF
                     KQFMIFLCPTGHGFWGA"
     gene            789..1646
                     /locus_tag="VJC18_05090"
     CDS             789..1646
                     /locus_tag="VJC18_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44790.1"
                     /translation="MFGLPTKPTWMATTTLSGDQSVPVIHVNTCAPDLRVAVDDLVDR
                     AKKIQSRPDKYIRATGKTTAGDCRHTIVSTRINGILIQIGWDRGKFTGTDGEARSELY
                     DRARRKMAEREPRPTPTERLRDQLHKERTLKAEITQTRKTLDELQQSHAADGLATMTD
                     DQLKALLIDVSHMLRTKAGEIIFKEIVTKTLLHIGIDGPRYRRSRFFSLKQSGAKTFR
                     QQLEWYAGEIVDMIQTNQLLAMNTWLNCVDVFSSKRGHLRTPYGIASYAYEFIRGIRG
                     VAGSILQFD"
BASE COUNT          512 a          416 c          402 g          431 t
ORIGIN      
        1 acacaggcag gagtgcaaat aaggaccttg ccagtttcat tgtcgataag gccgcggaga
       61 tttttgatct cacaactctg ccagctgatt ttgccgacac tttggatcag atcatcaaca
      121 gtcctgatat gcccgcggca agtgagccca ttgaaaaact gttttatatt cagaatcaat
      181 tggagacaat ttacgcagaa aatggcctgg accttgaatt ttcctttcgc cccatcattg
      241 ccctgcctat cgccagtttt gtgaggctta atgtttatcg aagtgacctg ggtgatttgg
      301 agtattttaa acaattcatg atttttttgt gtcccacagg acacggtttt tggggcgcat
      361 aagcaacaaa tcactctttc atacgatatg actgctatgt actgacatcg tcaggtttgc
      421 aaaacgatgt tacgaaaatt gtgagcaaca tttttacaaa ggagatagtt atggctttaa
      481 cagtcgcacc aacagctgtg acaccggcga ttcaggaata gtggagtcag gtcttgactt
      541 gggacttgca gtgtgtgtgt gactcatgaa ccatttgtgg caggcaagtc ccaagtcaag
      601 acctgacccc actatttttg tggcaggcaa gtcccaagtc aagacccgac cccaacccac
      661 tacaatccgt gtccctcagg acacggattg tgtggccgag aggcaacaaa tcattcgctt
      721 tgacgataac ctgtaataag aatcatcatg cgactaagcc ccgaatttcg ttatcagaga
      781 ggtgcagaat gtttggacta ccaaccaagc ctacgtggat ggcaacaaca acactttctg
      841 gcgaccaatc tgtccctgtc atccatgtta acacttgtgc gccagatctg agagtagcag
      901 tggatgattt ggttgaccgc gccaaaaaga tccagtcaag gccggataaa tacatcaggg
      961 caaccggcaa aaccacagcg ggagattgtc gacacacaat tgtgtctact cgtattaatg
     1021 gaatcctcat acagatagga tgggaccgtg ggaaattcac tggcaccgat ggagaggcaa
     1081 gaagcgaact ttacgacagg gcaagacgta aaatggcaga acgagaaccg cgacccacac
     1141 ccacagaaag attgcgcgat cagctacata aagagaggac cctcaaggca gaaataacac
     1201 agacaagaaa aacactcgat gaattgcaac agagccatgc cgcagacggt ctcgctacaa
     1261 tgacagatga tcaattgaag gccctgttga tcgatgtgtc ccacatgtta agaacaaagg
     1321 ctggtgaaat catattcaaa gaaatcgtca ccaaaacatt attacatatt ggcattgatg
     1381 gaccacgata cagacggtcc cgttttttta gtctaaaaca aagtggggcc aagacgttcc
     1441 gacaacagct ggaatggtac gcgggtgaaa ttgttgacat gatccagacc aaccaactcc
     1501 ttgccatgaa cacctggctg aactgtgtgg atgtcttttc ttcaaaaaga ggtcacctgc
     1561 gaacccctta tggaatcgcc agctacgcct acgagttcat acggggaatc cgaggggtgg
     1621 cagggtcaat tcttcaattt gattaactaa cgattttatt gatttctttt cgatttgact
     1681 ggccaagctc atcgcattct gctaacctgt cttcagaatg ctggatacac tcacagtcga
     1741 tctcctccat ccccatgacc g
//
LOCUS       DATDUN010000366         1471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_434159, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000366 DATDUN010000000
VERSION     DATDUN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434159"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VJC18_05095"
     CDS             <1..425
                     /locus_tag="VJC18_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44791.1"
                     /translation="QCLHGEGSVDCEILGWQELATLFVYKGMFAEDKEADERTSWVGF
                     RERYCDVVLSDETLEAYTTALIVKIILACEWYGLDEKAGQLFDYICEHGMHHLEEGQA
                     FLGDDHNLGFLLDGICTYQEWQGRTFRWLEKLRERLGK"
     gene            complement(599..1078)
                     /gene="rsfS"
                     /locus_tag="VJC18_05100"
     CDS             complement(599..1078)
                     /gene="rsfS"
                     /locus_tag="VJC18_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HLD44792.1"
                     /translation="MSPKKSPPSLSQVIVKACQAKKGLDVQLLDLSQISSYAEQVIVV
                     STTSDRHAQTIADHILDQAHKTLALSPIGVEGYETGQWVLVDFGSVVCHVFCNELRYE
                     YHLEDMWPSVSPVLEDALPAFFKPKIKIKTKASPKTATKTKTTKTVAKKRARSNASK"
     gene            complement(1075..>1471)
                     /locus_tag="VJC18_05105"
     CDS             complement(1075..>1471)
                     /locus_tag="VJC18_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HLD44793.1"
                     /translation="ANAFVVCDLPGLIEGAHQGKGMGDRFLKHAQRTRVLLHLVSLAP
                     DETRPAIDRYRVIEKEITKYFDDSKKRKRIIVLTKSDLVDNTVVTDVLKEFKKAKIKA
                     PLIVISAATRTNVNLVLDALVKELETNPK"
BASE COUNT          348 a          303 c          432 g          388 t
ORIGIN      
        1 ggcagtgttt gcatggggaa gggtcagttg actgtgaaat ccttggttgg caagagctgg
       61 cgaccctgtt tgtgtacaag gggatgtttg cggaggacaa ggaagccgat gagaggacgt
      121 catgggtggg tttcagggag agatattgcg atgtggtgtt gagtgatgaa acattggagg
      181 catacacaac agcgctcatt gtcaaaatca ttctggcctg cgaatggtac ggcctggatg
      241 agaaagccgg gcaattattt gactatatct gtgagcacgg gatgcatcat cttgaggagg
      301 ggcaggcttt tcttggcgat gatcacaacc ttggcttttt gctggacggg atctgcactt
      361 atcaggagtg gcaaggcagg acgtttagat ggttggagaa gttaagggaa aggctgggaa
      421 aatgagctca cgagcttgcg aatacatgag cgtacggaaa aacggcggat gaagaagtga
      481 aaagtacaaa atgaaaaatg aaaaatagga agggctgtca ttcctgcgta agcaggaatc
      541 cagaatagtt gaaaacatgg atcccagcat ccgctgggat gacaaatcgg tgtgtcattc
      601 acttgctggc gttggaacgg gctctctttt tggcaactgt ttttgtggtt tttgtttttg
      661 tggccgtttt gggagaggct ttggttttaa tttttatttt tggtttgaaa aaagcgggca
      721 gggcgtcttc caacacagga ctcacgctgg gccacatgtc ttcgaggtgg tattcatacc
      781 tgagttcgtt gcagaacacg tgacaaacca cggatccaaa atcgaccaga acccactggc
      841 ccgtctcata accctcaacg ccaatcggtg acagggccaa cgttttgtgt gcctgatcca
      901 aaatatgatc ggcgatggtt tgcgcgtgcc ggtcactcgt ggtgctgacg acgatcacct
      961 gttcggcata acttgagatt tgtgacagat ccaacaactg cacgtccaaa ccttttttgg
     1021 cctgacaggc cttgacgata acttgagaga gtgatggggg tgattttttg gggctcattt
     1081 tgggttggtc tccaattctt tgacgagggc atccagaacc agattcacat ttgttcgggt
     1141 tgccgccgaa atgacaatga ggggggcctt gatcttggcc tttttgaatt ctttgagcac
     1201 gtcagtcacc accgtattgt ccaccaaatc gcttttggtg agcacgatga tgcgtttgcg
     1261 ttttttggaa tcgtcgaaat acttggtgat ttctttttcg atcacccggt aacgatcgat
     1321 ggccggtctg gtttcgtcgg gtgccagact caccagatgc agaaggacgc gtgtgcgctg
     1381 ggcgtgcttc aaaaaacgat cgcccatgcc cttgccctga tgggctccct caatgaggcc
     1441 ggggagatca cagaccacga aggcgttggc t
//
LOCUS       DATDUN010000367         1502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_267952, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000367 DATDUN010000000
VERSION     DATDUN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267952"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VJC18_05110"
     CDS             <1..947
                     /locus_tag="VJC18_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLD44794.1"
                     /translation="QFDGLIQRPAQSAMGVKVKTAGQEFFDIKQEQVSVYWFDGFSQQ
                     GFVGSDNQVLLGESLYESLQFIPGDDEVVSITHPFADIGPTGEMEPSQRDFVLAGIFS
                     TGRLAFDESFALVSPSGLAQLSDDDFLEKEYYIFPRQWESLAQIQTLWQQGRGAQLGV
                     MKTWFDRNSNLFKAMKLEQLMYFVIFIFVFLISCFNMAALISIFGTSKSKETAILSAL
                     GLRLKQARLIFIQIGFVLGALGTLVGVGSALLLTVIAQKSGFRLPEAYGFTELPLAIN
                     VITLTGLLLGTPLFTALIACVPAYRLIHRDITQVMREN"
     gene            947..>1502
                     /locus_tag="VJC18_05115"
     CDS             947..>1502
                     /locus_tag="VJC18_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44795.1"
                     /translation="MNFILFLAKRYLSRTGVQGLRSWLIIVSTVGIFLASFMVLVALG
                     VLAGYQRVYHKAVLGFSAHLIIFNSEGLTDGDQQEITGFLKQSKMANVFSPYHFYETI
                     APSEDGPKPIIFKGVDETKMRQMYPIEFEDLPAGSSNNVFVGKDVLVRQPEPTSQGQL
                     NYLTNPHLGSERKTTLKSMPLQGIF"
BASE COUNT          336 a          350 c          401 g          415 t
ORIGIN      
        1 agcagtttga tggattgatc caacgccccg cgcagtccgc aatgggagtc aaggtgaaaa
       61 cggcagggca ggaatttttt gatatcaaac aggagcaggt gtcagtgtac tggttcgatg
      121 gtttttcaca gcaaggtttt gtaggcagtg acaatcaggt attgcttggc gaaagtcttt
      181 atgaatctct gcaatttatc ccgggggatg acgaagtcgt gtcgatcacg cacccctttg
      241 ccgacatcgg cccgacgggg gagatggaac cgagtcagcg ggattttgtt cttgcgggga
      301 ttttttccac gggccggttg gcctttgatg aatcctttgc gcttgtctca ccgagtggtc
      361 tggcacagtt gtcagacgat gattttttgg agaaggaata ttacattttt cccagacagt
      421 gggagtctct tgcccagatt cagaccctgt ggcaacaagg gcggggggcg cagctcggtg
      481 tgatgaaaac ctggtttgat cgcaacagca atttgtttaa ggccatgaag ctggaacagc
      541 tcatgtattt tgtcattttc atatttgtgt ttttgatttc gtgttttaat atggcagcgc
      601 tcatttccat ttttggaacg agcaaatcca aagagacggc cattttgagc gccttgggac
      661 tgagactcaa acaggccagg ttgattttta tccagatcgg ttttgttctg ggggccctgg
      721 ggacgttggt gggtgtgggc tcagccctgc tgttgaccgt cattgcgcaa aaaagcgggt
      781 tccgtttgcc cgaggcctat ggctttaccg aattgccttt ggcaatcaat gtgatcactt
      841 tgacaggtct gttattgggc acgcccctgt ttaccgcgtt gattgcctgt gttcccgcgt
      901 atcgattgat tcaccgcgac atcacacagg tgatgaggga aaattgatga acttcatttt
      961 atttttggcc aaacgctatc tttccagaac aggtgtccag ggacttcgtt cgtggctcat
     1021 catcgtttcc acagtgggta tttttctggc ctcattcatg gtgctggtgg ccctgggagt
     1081 tctggcagga taccagcgcg tctatcataa ggccgtactg gggttctctg cccacctcat
     1141 catttttaac agcgaaggac tcacagacgg tgaccagcag gagatcaccg gtttcctcaa
     1201 acaaagcaag atggcgaatg tgttttctcc ctatcatttt tatgaaacca tcgcccccag
     1261 cgaagatggt cccaaaccca tcatctttaa aggtgtggac gaaaccaaaa tgcgacagat
     1321 gtaccccatc gagttcgagg accttcccgc gggttcatcc aataacgtct ttgtgggcaa
     1381 agatgtgttg gtgcggcaac ccgaaccaac gagtcagggt caacttaatt acctgaccaa
     1441 ccctcatttg gggagcgagc gcaagaccac gctcaaatcc atgccgttgc agggtatctt
     1501 ca
//
LOCUS       DATDUN010000368         1220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_59754, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000368 DATDUN010000000
VERSION     DATDUN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59754"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VJC18_05120"
     CDS             <1..975
                     /locus_tag="VJC18_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLD44796.1"
                     /translation="RSKRSLGLDLKKSDAQTIIGRLIQKYDVLVEGFRSGVMSKLGLG
                     YETLKELNPALIYCSITGYGQTGPYSDRAGHDINFVALSGSASYTGRKESGPLPLGTQ
                     MSDLAGSYNAALGILAAVVSRKLTGRGQHIDVSMSDGMLALNVIAGAGFAALGLEPDY
                     EDSILNGGGTYDYYQTCDGRYFSVGCLEPKFFKRLCEAAGCQNKNTVTKKELKNIFKT
                     KTFDEWREIFANSDACVEPVLKFSEVAEHPHFRARGMFVQVDKRDGNTQRQIAFPVKF
                     SETCADYKHVGPTPGHDTRQILTEAGFLSEEISEFFRTGVVAAGQKIY"
     gene            1048..>1220
                     /locus_tag="VJC18_05125"
     CDS             1048..>1220
                     /locus_tag="VJC18_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLD44797.1"
                     /translation="MFKDLFARKSVAMLEAEMKGENRLRRILGPVALTNLGIGCIIGT
                     GIFVLTGVAAHDKA"
BASE COUNT          306 a          260 c          340 g          314 t
ORIGIN      
        1 cgttccaagc gctccctggg gctggatctc aaaaagtccg atgcccaaac catcattggt
       61 cgactcatcc aaaaatacga tgttttggtt gagggttttc ggtcgggcgt gatgagtaaa
      121 cttggtttgg gttacgaaac cctcaaagag ctcaacccgg ccctgattta ttgttcgatc
      181 acgggttacg gacaaacggg accatacagc gatcgtgcgg ggcatgatat taattttgtg
      241 gccttgtctg gatcggctag ttacacaggc cgcaaggaga gtggccctct tcccttgggg
      301 acacagatgt cagatttggc aggttcctac aatgcggcct taggcattct tgctgctgtg
      361 gtctcgcgga agctgactgg caggggccaa cacatcgatg tcagcatgag tgacgggatg
      421 ctggccctca atgtcattgc aggggccggc tttgcggctc tgggattgga acctgactac
      481 gaggactcta ttctcaatgg tggcgggacc tacgactatt accaaacatg tgatgggcgc
      541 tatttttcgg tggggtgcct ggaacccaaa ttttttaaaa ggctttgtga ggccgcgggt
      601 tgtcaaaata aaaacacggt aacaaaaaaa gaactgaaaa acatttttaa gacaaagacg
      661 tttgacgaat ggcgagagat ctttgccaat agcgatgcct gcgttgagcc tgttctcaaa
      721 ttttcggaag tcgcagaaca ccctcatttt agggctcggg gcatgtttgt gcaggttgat
      781 aaaagagatg gtaacacaca gaggcagatc gcttttccgg taaaattttc ggaaacctgt
      841 gctgattata aacatgtcgg gccgactcct ggtcatgaca cgaggcagat cctcaccgaa
      901 gccggatttt taagtgaaga gatatcagaa tttttcagga caggtgttgt tgctgctgga
      961 cagaagattt attgacagcc ttaataaaat acccacatac tgctcacgtg atttctgaat
     1021 ttcacaatta atgagggagg agttattatg ttcaaagacc tctttgctcg aaaatcggtg
     1081 gcgatgctgg aggcagaaat gaaaggtgag aatcgtttgc ggcgcattct gggccctgtg
     1141 gcgctaacga atttggggat tggttgcatc atcgggacgg ggatctttgt gctcacaggt
     1201 gtggcggccc acgacaaggc
//
LOCUS       DATDUN010000369         3905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_474187, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000369 DATDUN010000000
VERSION     DATDUN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474187"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..694
                     /gene="nrdR"
                     /locus_tag="VJC18_05130"
     CDS             242..694
                     /gene="nrdR"
                     /locus_tag="VJC18_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HLD44798.1"
                     /translation="MKCPTCQHPESKVIDSRVSNEANNIRRRRECEQCGQRFTTYERI
                     EDFLPMVIKKDGRRQVFDRLKILEGVKKACEKRPVSVEKISELVSQIEKKIFEKDAKE
                     IPSTEIGSLVMDKIKFLDHVAYVRFASVYREFKDINEFMKELQGLLKK"
     gene            696..1847
                     /gene="ribD"
                     /locus_tag="VJC18_05135"
     CDS             696..1847
                     /gene="ribD"
                     /locus_tag="VJC18_05135"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HLD44799.1"
                     /translation="MNNESRTRNKELRSDADHMLMALKLARKGAGRVSPNPLVGCVIV
                     KNGQVVGKGYHKKYGDYHAEVNALKMAGHKARGATLYVNLEPCFHYGKTPPCVDAVIA
                     AGIKRVVIAMQDPNPKTDGRSITKLKRHGIEVCVGVCAKEARELNQYFIHAMTHKQPY
                     VIVKVAQSLDGMITAQKGQRTQITGKRAVDYVQRLRSEVSAVLVGVNTVRVDDPLLTV
                     RNPKKPQPKRIILDSNLSLPHTVNLFGASGGQVILVCALPARHKKVLAFTKRGVVVIS
                     DVPRDRQGLDLKYVLKSLYDLGLRSVLVEGGAKVFRSFLTQKLADEWQIITAPVVYGE
                     CGLAWMSPDFFARLQLQETRTLGRDKLIVFKCSRVHERLKVQGLRRKVK"
     gene            1959..2543
                     /locus_tag="VJC18_05140"
     CDS             1959..2543
                     /locus_tag="VJC18_05140"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HLD44800.1"
                     /translation="MFTGIITNIGTIVNWKPAKDGGRMGVRPQKKYPKIKMGESIALN
                     GCCLTVVGYKKGLIDFDISDETIRKTAFLEIGKGRRVNLERAMMAHSALGGHFVLGHV
                     DGVGSIKAIKKNPGSVEYTIAYPKKFAHLMIEKGSVSVDGISLTVCNLTKSTFEVYII
                     PHTLKETIMSEYTVGQKVNLEFDVLGKYVARLKA"
     gene            2693..>3905
                     /locus_tag="VJC18_05145"
     CDS             2693..>3905
                     /locus_tag="VJC18_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44801.1"
                     /translation="MAKIRFFISYFLILSLTVIFVWGACGGGDSDSDGDDVVIVECDG
                     ATSGSFADDGGQFELTIPDSGVSDCLYISPNVTSSSDTTDLVGENETLLSGSAFELSL
                     NTEGDNDAEASPDEDLLITIAFDESLVPDAGDVDALHIYANVAFEDEDGNEVVVPVFG
                     DVDDGELIINTKGLYNVTRWVVVYNPDMGSDIDTTEPTVYLTDASQLTDEPGYSGGWN
                     QNDWCAMYDASDAVLRQTIATALSKAVGELTGADISTYIKTNVSLAAKIAGLTYADGG
                     FREPNLYPYEPSSSPSQVNICSDATRDYYKVIIAGSSHYQNPSTVKGKNPFGILYLGS
                     SEVNDAITGAAGSTFDAIAHEMFHAIFAGYDLSFLRRGLGMNEGMASTLGHTLGWADY
                     FDRVSINSTDWD"
BASE COUNT         1016 a          863 c         1043 g          983 t
ORIGIN      
        1 cggcgctcac cacgcgcggc atgaaggaat ccgagatgaa aatcattgct ggctggatga
       61 ctgatgtcat caccaaaccc aacgacgagc aactgcatca tctcgttctt gccaatatca
      121 gggaactcac ggccaggttt gggttgtatg tttagcaacg tggatcgtta atcgtgaatc
      181 ggggatcgaa aatgtttttt aagatccact atccacgaac cacgaaccac gatctacgag
      241 tatgaaatgt cccacctgcc agcaccccga aagcaaggtc attgactccc gcgtatcgaa
      301 cgaggccaat aacatcagac gtcggcgtga gtgcgagcag tgcgggcaga ggttcaccac
      361 gtacgaacgc atcgaggatt ttttgccgat ggtgatcaaa aaggatggcc gtcgtcaggt
      421 ttttgaccgt ctcaagatcc tcgaaggtgt caaaaaggcc tgcgaaaaac gccctgtctc
      481 agtcgaaaag atcagcgagc ttgtctctca aatcgaaaaa aaaatattcg aaaaagacgc
      541 caaggaaatt ccctccacag aaattggatc attggtcatg gataaaatca agtttctgga
      601 ccacgtggcc tatgtccgtt ttgcctccgt ttaccgcgag ttcaaggaca tcaatgagtt
      661 tatgaaggaa ctgcaggggc ttctcaaaaa ataaaatgaa taatgaatct cgaacaagga
      721 acaaggaatt gcgaagtgat gcagatcaca tgttgatggc attgaaactg gcccgtaaag
      781 gagctggtcg cgtgtcgccc aatcctcttg tgggatgcgt gatcgtgaaa aacgggcagg
      841 tggtggggaa gggttaccac aaaaaatacg gtgattatca cgccgaggtc aatgccctca
      901 aaatggctgg ccacaaggct cgcggggcta cgctgtatgt gaatctggag ccatgttttc
      961 attacggtaa aactcctccc tgcgttgacg cagtcattgc cgctggcatc aaacgtgtag
     1021 tgattgccat gcaagaccct aatcccaaaa cggatggccg atccatcaca aaactcaaac
     1081 gacatggtat tgaggtctgt gtgggtgttt gtgccaaaga ggcgcgggaa ctgaatcagt
     1141 attttattca tgcgatgact cacaaacaac cttatgtcat tgtcaaagtg gcgcagagtc
     1201 ttgatgggat gattactgcc caaaaaggtc agcggaccca aatcacgggt aaacgagccg
     1261 tggactatgt gcaaagactc agatccgaag tgtctgccgt gcttgtgggt gtgaatacag
     1321 tgcgcgttga tgatcctctg ttgacagttc gtaatcccaa aaagcctcag cctaaaagaa
     1381 ttattctgga ttcgaacttg tcactccctc acactgtcaa cctgtttggt gcgtccgggg
     1441 gacaggtgat tctggtttgc gccttgccag cgcggcataa aaaagttttg gcatttacaa
     1501 agcgtggcgt ggttgttatt tctgatgtcc ccagagacag gcagggcttg gacctcaaat
     1561 atgtcctcaa atctctctac gatttgggat tgcgctctgt attggttgag gggggcgcca
     1621 aggtttttag gtcatttcta acgcagaaac tagcggatga atggcagatc atcacagcgc
     1681 ctgtggttta tggggagtgt ggtttggcct ggatgtcgcc ggatttcttt gccagactgc
     1741 agttgcagga gacaaggacg ttggggagag ataaattaat cgtatttaag tgttcacgtg
     1801 ttcacgaaag gctaaaggtc caaggcttaa ggcgtaaggt taagtagggg gggctgaata
     1861 tcgaatcatg aacaaggaac aaggaatttc gaaatgaagg cttttggtct cgacattcga
     1921 cattccttgt tcgatattca atattcgagt ttgctgttat gttcacagga atcattacaa
     1981 atatcggaac gattgttaac tggaaacccg ccaaagacgg ggggcgcatg ggggtgagac
     2041 cccaaaaaaa ataccccaaa atcaaaatgg gcgagagcat tgcgctcaat ggttgttgct
     2101 tgacggtggt ggggtacaaa aagggtctga tcgattttga catttctgac gaaaccattc
     2161 gcaagaccgc ttttttggaa ataggcaaag gcaggcgcgt caatcttgaa cgggccatga
     2221 tggcccattc ggcgttgggt ggtcattttg tgttggggca tgtggatggc gtggggagca
     2281 tcaaggccat caagaaaaat cccggttccg ttgagtacac cattgcgtac cccaaaaaat
     2341 tcgcccatct gatgatcgaa aaagggtctg tctccgtcga cggcatttct ttgaccgttt
     2401 gtaacctcac aaagagcact ttcgaagtgt acatcattcc tcacacactt aaagagacca
     2461 tcatgtcgga atacactgtt ggacaaaaag tcaatctgga attcgacgtg ctgggcaaat
     2521 atgtggcgag gctcaaggcg taaagggttg tctcacaccc ctaataataa cttgaaaata
     2581 cggcaaaaaa tgcctaacct tccccgtccg taagggtcgg tctcgaccga cccctacaat
     2641 ttcatttggg gtagcaaaaa aaaaccaaag caatccaata aaggggggag cgatggcgaa
     2701 gatcagattt ttcatttctt attttctaat tttatcgttg accgttatct ttgtctgggg
     2761 ggcgtgtggg ggcggtgatt cggattcgga tggtgacgat gtcgtcattg tggaatgtga
     2821 tggcgctacc agtggcagtt ttgccgatga tggtggtcag tttgaactga caatacctga
     2881 ttcgggggtg agtgattgcc tctacatttc tcccaacgtg acttcgtctt cagacaccac
     2941 agatcttgtg ggtgaaaacg aaacacttct ctcgggttcc gcctttgaac ttagccttaa
     3001 taccgagggt gacaatgatg ccgaagcctc acctgatgaa gacctcttga tcacgatcgc
     3061 ttttgatgag tcccttgtgc ctgatgctgg tgatgtcgat gccctgcaca tctacgccaa
     3121 tgtggcgttt gaggacgagg atggcaatga ggtcgtagtc cccgtgtttg gtgatgtgga
     3181 tgatggcgaa cttatcatta acaccaaagg gctttataat gtcacacgtt gggttgtggt
     3241 ttacaatccc gatatgggat cggatattga cacgacggaa ccgactgttt acctgacaga
     3301 tgcctctcaa ttgacggatg aaccaggcta ttcgggcggg tggaaccaga atgattggtg
     3361 cgccatgtac gatgcgtcag atgccgtgtt gagacaaacc atcgccacgg cgctcagcaa
     3421 ggctgtggga gaactgacgg gtgccgatat ttcaacatac atcaaaacga atgtctctct
     3481 ggcggccaag attgccggtc tgacttatgc cgacggggga ttcagggaac ccaatctgta
     3541 tccctatgag ccatcatcga gtccctcgca ggtcaatatc tgttcggatg ccaccaggga
     3601 ctattataaa gtcatcattg cgggcagttc gcactatcag aatcccagca cggttaaggg
     3661 taaaaatccc tttgggatat tgtatttggg aagctctgag gtgaacgatg ccattacggg
     3721 tgccgcggga tccacgttcg atgcgatcgc gcacgagatg tttcacgcga tttttgcggg
     3781 ttatgattta tctttcctcc ggcgaggtct gggcatgaac gagggtatgg cctccacact
     3841 gggtcatacg ttgggatggg ccgattattt tgatcgcgtc tcgattaatt ccacggattg
     3901 ggact
//
LOCUS       DATDUN010000370         3189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_267996, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000370 DATDUN010000000
VERSION     DATDUN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267996"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3189
                     /locus_tag="VJC18_05150"
     CDS             <1..>3189
                     /locus_tag="VJC18_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HLD44802.1"
                     /translation="VLPTIHHISTRERVSSIASVGEVSHTISQFETPHTATPKTQLLS
                     NGRYALMVTNAGGGYSRWGDFEITRWRSDTTRDAFGTFCYIHDVDSNQLWCNTYHPLG
                     GVVETYQATFTVDRAVFQRQDHDIETETEIIVPPEDDVEVRRLTVINRSARVRKLNVT
                     SYIELSMAPHQADKQHPAFNKLFIQTEGVPGQQAVLAYRRSRGDGAPPICVAHCFTQD
                     CLVTEGMQFETDRRQFIGRGRTLANPMGAGQKLGNSQGFVLDPILSLRQGLILEPGQR
                     VQISLVLAVAPSRELVLNLISKYSSAHTVEHAMDTVWASAQVELRLLRIQSEEARRFQ
                     LLASHLLYPNYLLRAPAKRIMENGKGQSGLWAYGISGDLPIVLVVIGEARDIGLIRQI
                     LQAHNYWHRHGLKSDLIIINEEAHSYEQPLREKLERMMLELTTSTGKEQPGGVYLRSA
                     GLIPKEDLILMRAVANVVLIAARGGLPQQMGEATDAPELPKLLVRKQSDRDRATALPF
                     LELNYFNSLGGFTQDGREYAIYLGPDLNTPAPWVNVIANPSFGTVISETGAGFTWQGN
                     SQRNRLTEWSNDPVADPSSEAIYIRDEETGRLWTPTASPIREKSAYRTKHGIGYTLFE
                     HNSHGINQELTVFVPVNDQGGEPVKLQKLRLFNDSGRPRKLSLTYYVEWILGEARESS
                     QMHIVTHWDDELRIMTARNSYHPDYPQAIAFVALNIPAEAYTGDRSIFLGRNRSPQNP
                     LALEHVRLSGRSGAGLDPCAALQVTVTLAAGEATELVCLLGQTPTVARAQALVLNYRR
                     KHSFETAFNRTKGWWDQNLNTIQVQTPELSTNLLVNRWLLYQTLSCRIWGRSAFYQSG
                     GAFGFRDQLQDVMALLYMQPSLARAHILLAANRQFREGDVQHWWHPPGGAGIRSRISD
                     DLLWLPYVVAQYVYVTNDQGILNEIISFIDAPELDQNQHELFLMPVQATEQVSLFGHC
                     QRAVSRAQRFGAHGLPLMGTGDWNDGMNLVGALGRGESVWLAWFMIDVLKRMAEMSVL
                     QSETELARTYLQARDKLIQSTEGFAWDGEWYLR"
BASE COUNT          747 a          816 c          850 g          776 t
ORIGIN      
        1 cgttctgccc acgatccatc acatctccac acgagagcgc gtatcttcaa tagccagtgt
       61 gggtgaagtg tctcacacca tcagccagtt tgagactcct cacacagcaa cacccaaaac
      121 ccagcttttg agtaacggtc gctatgccct catggtcaca aatgccggtg gcggttacag
      181 caggtggggt gatttcgaaa tcacacgttg gcgatcagat accaccaggg atgcttttgg
      241 aaccttctgc tacattcatg atgtcgattc gaatcagttg tggtgtaaca catatcaccc
      301 cctaggagga gttgttgaga cctatcaggc gacgtttacc gtcgaccgcg ctgtgttcca
      361 acgacaagat cacgacatcg aaaccgaaac agaaatcatt gtgccgccag aagatgatgt
      421 tgaagtgcgt cgcctcacag tcatcaaccg ttccgcgcgt gtcagaaaac tcaacgtcac
      481 gagttatata gaactttcta tggcgccaca ccaggccgac aaacagcatc ccgcctttaa
      541 caaacttttc attcaaaccg aaggtgtgcc tggtcagcag gccgtattgg cttaccgcag
      601 gtctcgcggt gacggtgcgc ccccgatttg tgtcgcacat tgcttcacgc aggactgtct
      661 tgtgactgag ggtatgcaat ttgaaacgga tcgtcgccaa ttcattggac gagggaggac
      721 actcgccaat cccatggggg ccgggcagaa gcttggcaac tctcagggtt ttgttctgga
      781 tcccatcttg agtcttcggc agggcttgat tttggagccc ggccagcgcg ttcagatttc
      841 tctggtgttg gctgttgccc cttcgcgcga gctcgtcttg aacctcatca gcaaatacag
      901 ttcagctcac accgttgaac acgcgatgga tacggtgtgg gcgtctgccc aggtggaatt
      961 gagactgctc cgtattcaat ccgaggaggc gcgcagattc caattattgg ccagccatct
     1021 cctttatccg aattatcttc ttcgtgcacc ggccaaacgc atcatggaaa atggcaaagg
     1081 tcaatccggc ttgtgggcgt acgggatttc gggtgatctt cccatcgtgc tcgttgtcat
     1141 tggtgaagcg agggacattg gtttgattcg tcagatatta caggcccata attattggca
     1201 caggcacggg cttaaatccg atctgatcat catcaacgaa gaagcccaca gctacgaaca
     1261 acccttgcgc gaaaaactgg aacgcatgat gctggaactc actaccagca caggcaaaga
     1321 acagccgggc ggtgtttatc tgcgcagtgc aggactcatt cccaaggaag atctgatcct
     1381 catgcgtgcc gtggcaaatg ttgtgttgat tgcggcccgt ggtggcctgc cacaacaaat
     1441 gggagaggca acagacgcac cagaacttcc caaattactg gttcgaaagc agtcagatcg
     1501 tgatcgcgca acagccttgc cttttttgga actgaattac tttaacagtc tgggtggttt
     1561 cactcaggat ggacgcgaat atgcaatcta tctgggcccc gatctcaaca caccggcacc
     1621 ctgggtgaat gtgatcgcca atccctcttt tggaacagtg atcagcgaaa cgggcgctgg
     1681 tttcacgtgg cagggaaaca gccagcgtaa ccgtctgacc gagtggtcga atgatcctgt
     1741 tgccgacccg tcatctgagg cgatttacat tcgtgatgaa gagaccggtc gcctctggac
     1801 gcccacggcg tcaccgattc gcgaaaaaag tgcttaccga accaaacacg gtattggtta
     1861 cacgttgttt gaacacaaca gtcatggcat caatcaagaa ctcactgttt ttgttcctgt
     1921 gaatgatcag ggaggagagc cggtcaaact tcaaaaactc agactcttta acgattctgg
     1981 cagacctcgc aaactctcgc tcacctatta tgtggaatgg atattgggag aggcgcgcga
     2041 atcttcgcaa atgcacattg tgacccactg ggatgatgaa cttcggatca tgacagcccg
     2101 taacagttat catcctgatt acccgcaggc gatagcgttt gtggccttga atattcctgc
     2161 cgaagcttac acgggtgatc gttcgatttt tttgggaaga aatcgttccc ctcaaaatcc
     2221 actggccttg gaacatgtgc ggctctcagg ccgatcgggg gctggactcg atccctgtgc
     2281 ggccctgcaa gtgactgtga cactcgctgc tggcgaagca accgagcttg tttgcctgtt
     2341 gggacagact cccactgtgg cacgagctca agcgctggtt ttgaactacc gcaggaaaca
     2401 ctcttttgaa acagccttca acagaacaaa aggctggtgg gaccagaact tgaacaccat
     2461 ccaggtgcaa acccctgagt tgtcgaccaa tcttctcgtc aaccgctggt tgttgtatca
     2521 aacgctgtcg tgccgcattt ggggaagatc ggctttttac caatccggcg gtgcgtttgg
     2581 ttttcgtgat cagttgcagg atgtgatggc cctgctttac atgcaaccat ctttggcgcg
     2641 tgcacacatt ttgcttgcgg ccaatcgtca gttcagggaa ggtgatgtac aacattggtg
     2701 gcacccaccc ggtggtgcgg ggatccgttc gcgaatctca gatgatcttt tgtggttgcc
     2761 gtacgtggtc gcgcaatatg tttacgtgac aaacgatcag ggcattctca acgaaatcat
     2821 ttcttttatc gacgcgcctg aattggatca gaatcaacac gaactcttct tgatgcccgt
     2881 gcaggccaca gagcaagtct ctctttttgg gcattgtcag cgagcggtat cacgtgcgca
     2941 acgatttgga gcacacggtt tgcctctgat gggtacagga gactggaatg acgggatgaa
     3001 cctggtgggt gctttgggaa ggggtgagag tgtctggttg gcgtggttca tgatcgatgt
     3061 gctcaagcga atggccgaaa tgtctgtgtt gcagagcgag acggagctgg cacgcacgta
     3121 tctgcaagcg agagacaagc tcattcaaag caccgagggt tttgcctggg atggcgagtg
     3181 gtatcttcg
//
LOCUS       DATDUN010000371         1226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_434554, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000371 DATDUN010000000
VERSION     DATDUN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434554"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..711
                     /locus_tag="VJC18_05155"
     CDS             196..711
                     /locus_tag="VJC18_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44803.1"
                     /translation="MEVFAYVQAPLTAFLFWRLARRNVVGDLIAGSIVGMFVEFASEP
                     LWNYHFKLTIWKDVSPGIIMGYGVLFALAVFFSEKLYKWWFKESTIKDYDKRIFITDV
                     LAAAWIALPLEKIGLITKVWDYNQDVLKWSTIYIPIVDLQLEVFVGYALLMLIAPTFV
                     RYWQKGFERDL"
     gene            750..>1226
                     /locus_tag="VJC18_05160"
     CDS             750..>1226
                     /locus_tag="VJC18_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44804.1"
                     /translation="MNFRKHMGTMFVHQIEFMSKIRMQILVACVVAFIGTALLWFMPN
                     SPFPFTFCLVTGFSAVFHFFIFIIFKKIQPAHVVFVLVMNVVLTPFIVHLSGGIASPF
                     MLLFSSILLTALLFGMRSTTSLAIVIIPYLVVIMLEYWGILLPPTLSAATIYQSGLA"
BASE COUNT          268 a          251 c          311 g          396 t
ORIGIN      
        1 ttatcactat gagatactgg actggagccc ggcgcgcgat ccgatttttg gcatgcccta
       61 cgaggtactg atcggctatg tgttactgat gatgatcgcg cccacgttcg tgcggtactg
      121 gcatgccacg ttcgaacgtg agatagaaaa gattcgataa aattacctgt gaggtgacca
      181 atgttgttat cattcatgga agtgtttgcg tatgtgcagg ctcctctgac cgcgtttttg
      241 ttttggagat tggcgcggcg caatgtggtg ggtgatttga tcgcgggatc cattgtgggc
      301 atgtttgttg aatttgcatc tgagccattg tggaattacc atttcaagct aacgatctgg
      361 aaggatgtat cacctggtat tatcatgggg tacggggtgc tgtttgccct ggcggtgttt
      421 ttctcggaga aactgtataa gtggtggttt aaggaatcta ccatcaaaga ctacgacaaa
      481 cgtattttta tcaccgatgt gcttgctgct gcctggattg cgttaccgct tgaaaagatt
      541 ggcttgatca cgaaggtgtg ggattataat caggatgttc taaagtggag tacgatttat
      601 atcccgatcg tggatttgca attggaagta tttgtgggat atgccctgct gatgttgatc
      661 gcgcctactt ttgtgcggta ttggcaaaaa ggatttgaac gagatctata aacatgatca
      721 acaaagccaa cgatgtctat catgcgttta tgaatttcag aaaacatatg ggtacgatgt
      781 ttgtgcatca gatcgaattt atgagcaaga tccgtatgca aatactggtc gcctgtgtgg
      841 ttgcctttat cggtacggcg ctcctgtggt tcatgcccaa ttcgcccttt ccatttacct
      901 tttgtctcgt gaccggtttt tcagccgtct ttcatttctt catatttatt atatttaaaa
      961 agatccaacc tgctcacgtc gtatttgtgt tggtcatgaa tgtggtgctg acaccgttca
     1021 ttgttcacct gtcgggtggc atcgcttcac cgttcatgct attgttttcg agtatcctgt
     1081 tgaccgccct gttgttcggc atgaggagta ctaccagcct tgccattgtt atcataccgt
     1141 atttggtcgt catcatgttg gaatattggg gcatactcct tccaccgaca ctctcggctg
     1201 ccacgatcta tcaaagcggg ttggca
//
LOCUS       DATDUN010000372         4064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_434620, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000372 DATDUN010000000
VERSION     DATDUN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434620"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VJC18_05165"
     CDS             <1..493
                     /locus_tag="VJC18_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HLD44805.1"
                     /translation="PLLDERTQLMGQVYGVVNTANMVLHALTGVILRLAGIPITMSVI
                     PVMLAFSLGAFIAIPSLICIAIVKISSKVLDYTLFRSARECLYIPLSHEERTQGKSVV
                     DMIVNRVGKVGASFIVMGLISAGVTELVSWMGLFLILIWTILAIVITRRFRKLVSRKE
                     ELS"
     gene            490..987
                     /locus_tag="VJC18_05170"
     CDS             490..987
                     /locus_tag="VJC18_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLD44806.1"
                     /translation="MTPKQQHRYFIGIVSDTHGHKPPMLKTLFHNVDLIIHAGDIGDS
                     SVLDFLGAIAPVHAVRGNMDHIPEWSRLPETAIVTIGSHRFFVLHDLEELDFDPQTAG
                     FMAVIHGHTHQASHQRQGGVDYINPGSAAGIRSPHATAALVTITDNALTVSCINLDMT
                     KTHNP"
     gene            1375..1704
                     /locus_tag="VJC18_05175"
     CDS             1375..1704
                     /locus_tag="VJC18_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44807.1"
                     /translation="MKKYFSMILLTLCLSLLPPMLYAEGKVDQSSCSYNGRALYGKVQ
                     VVTAFPDLKVQKVTAFPDLKVEQVSAFPDSCGKWQMVDAFPDLKIQFVDAFPDITIQY
                     VTAFPGV"
     gene            complement(1701..2612)
                     /locus_tag="VJC18_05180"
     CDS             complement(1701..2612)
                     /locus_tag="VJC18_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44808.1"
                     /translation="MKTNHIQTIIFCFFVLFVMGACGGIGAGVNDALFDNAMVSEDGL
                     SQEIMTGESDEGDSDEEVLGQSDDVIPQDEFNEIPTAGQESAEGMDDGDYGNDSPTAD
                     QGNKATDANDEKDLDDPDYLETETGPTSLPVTIAKEEPEEGDACEPEEEGERFATSLD
                     VTASTPREAGEKIGPKGKKISTKIGTIASEALTEEGVDVEYVPGVKVIECRYNEEENG
                     YYWQKIPDNIVYEEYDLNLRFVDTGEEETTEFTPQGTMLLDQSLLEVSEETDVCVTLV
                     EKETNQKVSGTGLVISNKTVKQNAPKC"
     gene            2978..>4064
                     /locus_tag="VJC18_05185"
     CDS             2978..>4064
                     /locus_tag="VJC18_05185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLD44809.1"
                     /translation="MEQIAKKYRVLKKLGQGAMGEVYLVLPPHGEPVALKLLKSLEAE
                     ANKQALEQFENEFKVLKRLAHPNIGRIFDYGFDEQLNKVFFTLPWLQGQEIFEATKDI
                     TYETCEEYFVQTLRALNYLHQKKLIHCDLKPGNIYIENNKVILIDFGLTGYWGENIVG
                     TPTYLAPEIFHGKHHTVASDLYAVGVIFYNCLTRSQPFSGKDLQEVYDRHRCFTPPPA
                     TEVNPKVPKYMSDIIATLINKKPEERYPSAASVIEEIDAYSQTHYSVETEDTLLSYLP
                     TESEVPGNEENIMDIMAALEAFNAQHIDEPYHLILVQGKKNVGKGRLLTKIKNELQLA
                     KVSVEQIKSPLSEQQKEVLSNSTAVIID"
BASE COUNT         1006 a         1110 c          897 g         1051 t
ORIGIN      
        1 tcccctgctg gatgaacgca cacaattgat gggacaagtt tacggtgtgg tcaacacggc
       61 caacatggtt ctgcacgccc tgacaggcgt gattctgcgt ctggcaggga tcccgatcac
      121 catgagcgtg atccccgtca tgctggcctt ctccctggga gctttcatcg ccatcccctc
      181 tctcatctgc atcgcgatcg tcaagatttc cagcaaggtg ctcgattaca cgttgttccg
      241 ctcggccaga gaatgcctct acatcccgct gagtcatgag gaacgcactc aaggcaaatc
      301 ggtggtcgac atgattgtca atcgcgtggg caaggtgggc gcctctttca ttgtgatggg
      361 cttgatctct gccggagtca ccgaattggt atcctggatg ggactgtttt tgattctcat
      421 ctggacaatc ctcgccattg tgatcaccag gcgatttcga aaactcgttt cgcgcaaaga
      481 ggagttgtca tgactccaaa gcagcaacat cggtacttca ttggaatcgt ctccgacacg
      541 catggccaca agcctcccat gctcaagaca ctgtttcaca atgtcgacct catcatccac
      601 gccggggata tcggtgactc gtctgtattg gattttttgg gtgccatcgc gcccgttcat
      661 gccgttcgtg gcaatatgga tcacatccca gaatggtcac gactgccgga gacggcaatc
      721 gtcacgatcg gttcacaccg attttttgta ttgcatgact tggaagagct cgattttgac
      781 ccgcagaccg caggttttat ggccgtcatt catggacaca cacaccaggc atcacatcag
      841 cgacaaggcg gcgtagacta catcaatcct ggatcagccg ccgggatcag atcacctcac
      901 gccaccgcgg ccctggtaac cattactgat aacgctctta cagtttcatg catcaatctt
      961 gatatgacga aaacccataa cccataaccc ataacccatt accagcgatg tgccctgagg
     1021 gtgtgcaaca taaaagtggg tgattttgat tttgcccccc cccgcctgtt gtaaggcggg
     1081 ggtgcccgta gggcgggggt ggtagaaaat ttgggaattt cccatctctc agctgatcac
     1141 gatactacct ccccaccctg cgttgctacg cagggagccc ctccttatca ccgcaaggcg
     1201 ggatcccgtt tgcgggacaa aaaggagggg cggggcacca ttttacccac aaatatgtcg
     1261 cacatcccca actgagagag tgattgataa agtacaggga atgacgatat gctccgacaa
     1321 tgtgcatctg tttttctgat gtaccatgag tcaataaacc ccggggagac caaaatgaaa
     1381 aaatattttt cgatgatctt gctgaccttg tgtctgagtt tgcttccgcc gatgctgtat
     1441 gccgaaggca aggtagatca atcctcatgt tcgtataatg gccgcgcctt atacggcaag
     1501 gttcaggtgg tcactgcgtt tcctgacctc aaggtccaga aggtgacggc gtttcccgat
     1561 ctcaaggtgg aacaagtctc cgcttttccg gattcctgcg gcaaatggca aatggtggac
     1621 gcttttcctg atttaaagat tcaattcgtc gacgcttttc cggacatcac catccagtac
     1681 gtcacggcct tcccgggggt ttagcacttg ggggcgttct gcttcaccgt tttgttagaa
     1741 atcaccaggc cggtgccgga gactttttga ttggtttctt tttctaccaa ggtcacgcac
     1801 acatcggttt cctcactcac ctcgagcagt gattgatcaa gcaacatcgt cccttgaggg
     1861 gtgaattccg tggtctcttc ctcccccgtg tcgacaaatc tgagattgag atcgtactct
     1921 tcgtacacaa tattatcagg aattttttgc cagtaataac cgttttcctc ttcattgtag
     1981 cgacattcga tcaccttgac ccctgggaca tattccacat caacgccctc ttcggtgaga
     2041 gcctcggagg cgatcgtgcc tatctttgtg cttatttttt taccctttgg tccaatcttc
     2101 tcaccagctt cgcgcggtgt cgacgctgtg acatcgaggc tcgtggcaaa gcgttcgcct
     2161 tcttcttcgg gttcacacgc atcaccctcc tcgggttctt ctttagcaat cgtgaccggg
     2221 agagatgtcg gtcccgtttc tgtttccaag taatcgggat catcgagatc tttctcatca
     2281 ttggcatcgg tcgctttgtt tccctgatca gccgtcgggc tatcattacc gtaatcgcca
     2341 tcatccatcc cctccgcaga ttcctggccc gctgtgggga tctcattgaa ttcgtcttgg
     2401 gggatgacgt catctgactg cccgagcacc tcctcatcgg aatcgccctc atcactttcg
     2461 ccggtcatga tttcctgcga aaggccatct tcactcacca tcgcgttgtc aaataatgca
     2521 tcgtttaccc ctgccccaat gccaccacaa gcacccatga caaacagcac aaaaaaacaa
     2581 aatatgatcg tctggatatg atttgttttc atatccttta tataagcaat ccctatgcca
     2641 aaacacttcc taatatttgt atgcatgata ttgttttcct tatataaaaa aagtcgttcg
     2701 ttccataaac ctaatattca ggtgactata gacccctttt ttatcaaaaa gaaaaaagaa
     2761 caatgccgac catcttcttc tttacatctt attgaattca caatagtatc tggagtcacc
     2821 accattcctt gttattgttg tggtcttttg tcatcaataa acaataaaat acttgtggtg
     2881 tcaagtcatc atttgatgga taatgtgaac tggggtactt tggaagcttt tgcgttagaa
     2941 ttataagaag aacatcatcc caacggattg gtttttaatg gaacagattg ctaaaaaata
     3001 ccgtgtcctc aaaaaactgg gtcaaggggc catgggcgag gtttatctcg tcctaccacc
     3061 acacggagaa cccgtcgccc tcaaactttt aaaatcactg gaagcagagg ccaacaaaca
     3121 ggctttggag caattcgaaa atgaattcaa agtgctcaaa agattggccc atcccaacat
     3181 cggtcgcatc tttgattatg gttttgatga acagctgaac aaggtttttt ttacactccc
     3241 gtggttgcag ggacaggaaa tcttcgaggc cacaaaagac atcacctacg aaacctgcga
     3301 ggaatatttt gtgcagactc tgagggcact caattacctg caccagaaaa aactcattca
     3361 ttgtgatctg aaacccggca acatctacat cgaaaacaac aaggtcatcc tcatcgactt
     3421 tggcctcacc ggttactggg gcgagaacat cgtcggcaca cccacctatc tcgcacccga
     3481 gatttttcat ggcaaacacc acacggtcgc cagcgatctg tatgcggtcg gagtcatttt
     3541 ttacaattgt ctcacccgtt cacaaccctt ttctggcaag gacctgcagg aagtctacga
     3601 ccgtcaccgc tgtttcaccc caccacccgc cacagaggtc aatcccaagg ttcccaaata
     3661 catgagcgac atcattgcca ctttgatcaa caaaaaaccc gaggagcgtt accccagcgc
     3721 ggcctcggtg atcgaagaaa tcgatgccta ttcccagacc cattactctg tcgaaacaga
     3781 agacaccctg ctttcgtacc ttcccactga gtctgaggtc cccggcaatg aagagaacat
     3841 catggacatc atggccgccc tagaggcctt caacgcccag cacattgacg aaccctacca
     3901 cctcattctg gttcagggaa aaaagaacgt gggcaaaggg cgtctgctca ccaaaatcaa
     3961 aaatgaactg caactggcca aggtcagtgt ggaacaaatc aaatcccctc tgtcggaaca
     4021 gcaaaaagag gtcctcagca acagcacggc cgttatcatc gatc
//
LOCUS       DATDUN010000373         3613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_474323, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000373 DATDUN010000000
VERSION     DATDUN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474323"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VJC18_05190"
     CDS             complement(<1..203)
                     /locus_tag="VJC18_05190"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557172.1"
                     /note="regulates pyrimidine biosynthesis by binding to the
                     mRNA of the pyr genes, also has been shown to have uracil
                     phosphoribosyltransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HLD44810.1"
                     /translation="MKTLLSSAAFNTAIDKLGAAICDQQSDYQNLAFVGIHTRGVPLA
                     KRLTAYFHKQNKPVLQGLLDINL"
     gene            397..1278
                     /locus_tag="VJC18_05195"
     CDS             397..1278
                     /locus_tag="VJC18_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLD44811.1"
                     /translation="MTAHRKLMVVLGLILLFILSSSCGENESLPSPNPNITLSPGLYY
                     QNLQTKEWTRLATEASEVLIGDLNADGRDDIVGAWPSEGGLYLFDLVADEWTKLSSVG
                     DSLAVCDMTGDGRDDLVGSWGEQGIIYRDSADGTWSGSLGQSTDVMACADMDQDELGD
                     LVVAWNQELYSYLSTTKTWTKLSSGQPVDLALCDVTGDDVPDMLVSIENGVYYRDTSS
                     GIWSEKLGEQADHLACGDVDEDGKADVLVNYLTGTTGVWIHYSTTGIWASLTYEADDI
                     VLGDMNNDHHLDLVGVW"
     gene            1362..1559
                     /locus_tag="VJC18_05200"
     CDS             1362..1559
                     /locus_tag="VJC18_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44812.1"
                     /translation="MTKAHDHIIPLLSKRFQKKEPLSTTSRIMRHVVKETARMAANEN
                     KPKERGVVVDKTTPTDKSGMV"
     gene            1820..2140
                     /locus_tag="VJC18_05205"
     CDS             1820..2140
                     /locus_tag="VJC18_05205"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HLD44813.1"
                     /translation="MRDETINNNKWIGFLGEDDIVFLKRFVLCSGSLKDLAKEYGITY
                     PTVRLRLDRIIAKVQVMDREEYADEYEQILRVQYAEEKIDATTFQTLLQAYRQINKQT
                     GSLR"
     gene            2175..3077
                     /locus_tag="VJC18_05210"
     CDS             2175..3077
                     /locus_tag="VJC18_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44814.1"
                     /translation="MKNKILPIMVGLLFVAMAQAKAGETKVVTDISFDGQTFQAGKQI
                     ENPDNPAEKIIEVNVSGSGNDTVTVFSLDNPKIAETEYALTGQISYHNITTPGFLEML
                     NHFPQGQFFTRALSPEGPLGLIQASSPWRTFTLPFTTNKDIKAPSKIELNLSLPGGGT
                     IYLKSISLVEHTTGAKALVAPLASGTGDGPVWWSDRTAGWLGAMSGVFIGFAGALIGI
                     CSSKGRAKRLVQGIMFLTLGLGLMMVVMGIVAVIREQPYVVYYPLLLGGGIITVVFGA
                     LVKTIQKRYLDLELRKMSAVDAGR"
     gene            complement(3087..>3613)
                     /locus_tag="VJC18_05215"
     CDS             complement(3087..>3613)
                     /locus_tag="VJC18_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HLD44815.1"
                     /translation="GTKFYIKTSGDVNIGSGGSGTSTSSSGTNLQEIETGIELKVTPQ
                     ISVNDFIKLAINATESEADFTKAIDGVPAVIDNTAETNIMLKDGETTIIGGLFRVRDA
                     QTVKGIPGLMKIPLLGSLFKSKSKAREKSELLIFITPKIINSAVSTLPYFSEKDSVHN
                     PDENAKEQRLKKKK"
BASE COUNT          894 a          773 c         1004 g          942 t
ORIGIN      
        1 tacaaattga tgtcgagcag tccttgcaag acaggcttgt tttgtttgtg aaaatacgcc
       61 gtcaagcgtt tggccagggg gaccccacgc gtgtgaatgc caacaaaggc caggttttgg
      121 taatctgatt gttgatcaca aatggcggcg cccagcttat caatggctgt gttgaatgcg
      181 gcagaggaaa gtagtgtttt cataaactcc tcacatgaga gccctttgag cgcagggcgt
      241 gtcggtgtgt tggtgtgttg ttggaaagga aaagaacatt caaattgacg agtctatagg
      301 caaagcggcc agcaatgtca aattgaggag acgcacggac aagcgatgtt gactcggagg
      361 gtctgggagg tatatgaaac ccgtggggag cctcgcatga cagcacacag aaaattgatg
      421 gttgtattgg gcctgatttt acttttcatt ttatcgtcct cttgtgggga gaacgaatcc
      481 ttgccttctc ccaatcccaa tatcaccctt agtcctggcc tatattatca aaacctgcaa
      541 accaaggaat ggactcgtct ggcgacggag gcctctgagg tcctgattgg cgacctgaat
      601 gccgatggac gtgatgatat tgtcggggcc tggccatccg aagggggcct ttaccttttt
      661 gaccttgtgg ccgatgagtg gaccaagctg agttcagtgg gtgattctct cgctgtgtgc
      721 gacatgacgg gcgatggccg tgatgatctg gtgggttcat ggggcgagca aggcattatt
      781 tatcgtgatt ccgctgatgg cacgtggtcg ggatcactag ggcaaagcac agatgtgatg
      841 gcgtgtgcgg atatggatca ggacgagctg ggcgaccttg ttgtggcttg gaatcaggag
      901 ctctattcct atctgtccac cacaaaaacg tggacgaagc tatcatcggg acaaccggtt
      961 gatttggcgc tttgcgatgt gactggcgat gatgtgccgg atatgctggt ttcgattgag
     1021 aacggggtgt attatcgtga cacctcttcc ggaatctggt cggagaaact gggtgaacag
     1081 gcggatcatc tggcctgtgg tgacgttgat gaggatggca aagctgatgt tttggtcaac
     1141 tacctgacag ggacaacggg cgtctggatc cattattcca caacaggcat atgggccagc
     1201 ttgacctatg aggcggacga tatcgtgttg ggcgacatga ataacgatca ccatttggat
     1261 ctggttggcg tgtggtgatg gaaaaagggc gcaacttttg gcccaatgtt gcgataccgt
     1321 gttcgagagg caacagtccc cagaggacaa aggagccgtc tatgacaaaa gcacatgacc
     1381 acattattcc cttgttatca aagcggtttc aaaaaaagga gccgctctca accacctcac
     1441 gcatcatgcg gcatgttgtg aaggagacag ccagaatggc cgccaatgag aataaaccaa
     1501 aggaacgtgg cgttgttgtc gacaagacaa ccccaaccga taaatcgggg atggtgtaat
     1561 cgtatcgccg ggaggcatca tccggcctcc cgctgtaagt tttgagaggt gtccttaaat
     1621 attcttggca gactcgctct gtcgtgtgac tctgacaatt catcttttcg catgtcgccc
     1681 gcgccgaacc accaaatctt aatataatta aagttgacat aaacattatt tggtgtataa
     1741 ttaaattgtg ttttgataat attgagaata aaatgaaaac gaattttgtt taagtttgtt
     1801 gttgtctaaa ggagtcgaaa tgagagatga aacaataaat aataacaagt ggataggctt
     1861 tctaggggaa gatgacatcg tttttttaaa gagatttgtc ttgtgctcag gttctctaaa
     1921 agatttggcc aaggaatatg gaataactta cccaacagtg cgactgaggt tggatcgtat
     1981 cattgccaag gtacaggtga tggaccggga agagtacgca gatgaatacg aacaaattct
     2041 tcgtgtacag tacgcggagg aaaaaatcga tgccaccacg tttcagaccc ttttacaggc
     2101 ttatcgccaa atcaataaac aaacaggcag ccttcgctga aaagatcagc tacggcgagc
     2161 ccagggagga caatatgaaa aataaaatac tgcctatcat ggttgggttg ttgttcgtag
     2221 caatggcaca ggcaaaagcc ggggaaacca aggttgtgac cgatatttcg tttgacgggc
     2281 aaacctttca agctggcaag cagatagaaa acccagacaa tcctgcagaa aagatcattg
     2341 aagtgaatgt gtcgggcagt ggaaatgaca cggtgacggt cttttccctg gacaacccaa
     2401 aaatagcaga aacagagtat gccctcacag ggcagatctc ttaccataac atcacgacgc
     2461 ccgggttttt ggaaatgctg aatcattttc cccaagggca atttttcaca cgcgctttgt
     2521 ctccggaggg tccgttgggt ttgatacagg cgtcttcacc ttggcgtacg ttcacccttc
     2581 cttttaccac gaacaaggac atcaaggcgc catcgaaaat tgagctgaac ctgtctcttc
     2641 cgggcggcgg caccatctat ttaaaatcga tttctctcgt tgaacacaca actggcgcaa
     2701 aggcacttgt cgcacccctg gcaagcggca cgggtgatgg tcctgtctgg tggtctgata
     2761 gaactgcggg ctggttgggg gctatgagtg gcgtttttat tggttttgca ggggcgttga
     2821 taggaatttg ctcaagcaaa ggacgggcta aaagactggt ccaggggatc atgttcctca
     2881 ctcttggtct gggcctgatg atggttgtga tgggtattgt tgcggtgatc agggaacaac
     2941 cctatgtcgt ttattacccg ctcttattgg gtggcgggat catcacggtc gtttttgggg
     3001 cgttggttaa gacgattcaa aaacgatatc tcgatttgga attgcgaaaa atgagtgcgg
     3061 tggatgcggg acgctaggac gctgtttcac ttctttttct tcaagcgttg ctccttggcg
     3121 ttttcgtcgg gattgtgcac gctgtctttt tccgaaaaat agggaagcgt agaaacggcg
     3181 ctgttgatga tctttggcgt aatgaagatg aggagttcgc ttttttcgcg tgccttggat
     3241 ttgctcttga acagggagcc cagcaaggga attttcatga gtcccgggat acccttgaca
     3301 gtctgcgcgt cgcgtacccg gaacagtccg ccgatgatgg tggtttcgcc atccttgagc
     3361 atgatgttgg tctccgccgt gttgtcgatg accgcaggaa cgccgtcaat ggccttggta
     3421 aaatcggcct ccgattcggt ggcgttgatg gccagcttga taaagtcgtt gaccgaaatt
     3481 tgcggtgtca ccttaagttc gatgcctgtt tcgatctctt gcagattggt tcctgagcta
     3541 ctggtagacg tgccggagcc gccgctacca atattcacat caccgctggt tttgatgtaa
     3601 aacttggtgc cgc
//
LOCUS       DATDUN010000374         2697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_308902, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000374 DATDUN010000000
VERSION     DATDUN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308902"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VJC18_05220"
     CDS             complement(<1..370)
                     /locus_tag="VJC18_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HLD44816.1"
                     /translation="MINRNDESLSVRRQCELVGLNRSSLYYEPVLVSAETQLIMNRID
                     DIYTGCPFYGSRKISAQLRREGFSIGREAVATLMKELGLAAVYPKRNLSLRNQAHKVY
                     PYLLRDMPVSAANQVWSADIT"
     gene            complement(384..668)
                     /locus_tag="VJC18_05225"
     CDS             complement(384..668)
                     /locus_tag="VJC18_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44817.1"
                     /translation="MQRKIHGAEFKAKVALMSLREDMTLSQLASKFEVHPTLITKWRK
                     HLLANVTDVFLKKSENKANAEHLADELYKQIGQLKVENEWLKKKSDLIIG"
     gene            complement(754..1515)
                     /locus_tag="VJC18_05230"
     CDS             complement(754..1515)
                     /locus_tag="VJC18_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44818.1"
                     /translation="MGQPLFVGPQLVGADIAGETCAGHHDSPKLKAPTYAKASVGSRG
                     SWIACAPEGMDRGSTVHSSHLFIHPAILNERTAHGARRLSPNAGSFIERVRPHVPIQM
                     AGAKKGSKGTDEGTIPVASAGGTPTDEPKVLTLNRTSTITTVAGVALLLDRVYEIGTV
                     STTVDEKLLEIAIEVEGLGISDDASVALNGDVLAKLGLFCDLCDALIETDDTAFTTQV
                     EQWIEVHGTFTHEELDGFVSSLVDPSTRSTRSGQV"
     gene            complement(1832..>2697)
                     /locus_tag="VJC18_05235"
     CDS             complement(1832..>2697)
                     /locus_tag="VJC18_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44819.1"
                     /translation="DPWSAKAALDKACSLQLDSDEAAPTYKELYHRFRDKQITDQAEV
                     CLQSAFDTGVFDLEVVELMGLKSYRRGDLLIAKQCFVDIARHAPAQIQAIKNVGVICN
                     EQGDYRQAKQSLFYTLGLYDRLISLLDYFTEAGVEPAFQEEIAHFPKDLLAMLPYDDD
                     PKHEHNKNTFASTKQTLLRDRDAVYRYLKNIYLHEKKDIPVEILAHTPEDVFNSDYEK
                     FVTQTSMVTQQSLPEQSVGDPLSPRNRFYIGSQFITVYEIAFYSFVNECSLTFKQILP
                     AERKEKALVRR"
BASE COUNT          684 a          697 c          574 g          742 t
ORIGIN      
        1 atgtaatgtc ggcgctccac acctgattcg ctgcgctaac aggcatgtcg cgcaacagat
       61 acggataaac cttgtgcgct tggttgcgca ggctcaagtt ccgcttgggg tacacggctg
      121 caaggcccag ctccttcatc agagttgcaa ccgcttctct gccaatcgaa aaaccttctc
      181 tcctcagttg tgccgagatt ttacggctgc cataaaaagg acaacccgta taaatatcgt
      241 caatgcgatt catgatgagc tgcgtctcgg ccgatacgag tactggctcg taatacaacg
      301 acgagcggtt cagcccgacc aattcgcatt gccgcctcac actcaggctt tcgtcattcc
      361 tgtttatcaa agagcgtttc tgatcagccg ataataagat cagatttttt tttaagccac
      421 tcgttctcca ctttgagttg gcctatctgc ttgtaaagct cgtccgctag atgttcagcg
      481 ttggccttgt tttcggactt ctttaaaaag acgtccgtta cgtttgccaa cagatgtttc
      541 cgccattttg tgatcagcgt cgggtggacc tcaaatttgg aggccaactg actgagcgtc
      601 atatcctctc tcaaggacat caatgccacc tttgccttaa attcggctcc atgtatcttt
      661 ctttgcatat ttgactcctt aaaggagcca cggctttttg gtcaaatcac ctttcttaaa
      721 tttcctaaaa aatggtccag ttttggccgg ccactatacc tgccctgagc gagtggaacg
      781 agtcgaaggg tcaacaaggg aactcacaaa cccgtcaagt tcttcgtgcg taaatgtgcc
      841 atgcacctct atccactgtt caacctgtgt tgtaaaagca gtgtcatccg tctcaatcaa
      901 cgcatcacaa agatcacaaa acaaaccaag tttggccaac acgtccccgt tcagagcaac
      961 agaagcatca tcgctgatcc ccaaaccttc tacctctata gctatttcca acaacttctc
     1021 gtcaaccgtt gtggataccg taccaatttc ataaacacga tccagtaaca aagcaacacc
     1081 tgccactgtt gtgattgtgg acgtacggtt caatgtcaaa accttgggtt catcagtggg
     1141 tgtgccacct gcgcttgcaa ctgggatggt accttcatca gtaccctttg accctttttt
     1201 agcacctgcc atctgtatgg ggacatgtgg ccgaacacgt tcaatgaaac tgcccgcgtt
     1261 tggactcagg cgacgtgcac catgcgctgt gcgttcgttc agaatggccg gatgaatgaa
     1321 taaatgtgaa ctatggactg tcgatccacg atccataccc tctggggcac aagcgatcca
     1381 cgatccacga ctacccacag aagccttggc gtaggtggga gctttgagct ttgggctatc
     1441 atgatgccct gcacacgttt ccccagcaat atctgcccct acaagttggg gcccaacaaa
     1501 tagaggctga cccaacgcac tcgtttggcc caacaaacct tgattgaatg gcgttatagc
     1561 tttcatatta tcatagcgat tgatgaacac atccgtctcg aagctcacac atcgcttatc
     1621 gaaagattaa ttcgaaggta ccaattcaca ggtaccaatg acgcacatac gtacacatgt
     1681 gtgcacttac actggggttg tatcgtcagc tcaaacgaaa agttgcttgt tttttttgtg
     1741 tttgttgacc ggagggaacc ttccagaaag ctgtttttat taggctatta tccacaccaa
     1801 aatgtacctg gcacctctca tctggcacct ctcatcgcct gacaagagcc ttctcttttc
     1861 gttcagcagg aagaatttgc ttaaaagtga gactgcactc gttcacaaag gaataaaagg
     1921 cgatttcgta gaccgttatg aattgactgc ctatgtaaaa tctatttctc ggtgataaag
     1981 gatccccaac agattgttcg gggagtgatt gctgtgtcac cattgaggtt tgagtcacga
     2041 atttttcata atcactgtta aacacgtctt ccggagtgtg agccaatatt tccactggaa
     2101 tatctttttt ctcatgtaaa taaatgtttt tcaaatagcg ataaaccgcg tccctgtccc
     2161 tgagcagagt ttgtttggtg ctggcaaatg tgtttttgtt atgctcatgc ttggggtcgt
     2221 catcataagg caacatggcg agcaagtctt tgggaaagtg cgcgatctct tcctggaaag
     2281 caggctcaac accagcctct gtaaaataat ccaaaagcga tatgagccta tcatataacc
     2341 ccagcgtgta aaatagagat tgctttgcct gtcgatagtc accctgttcg ttacaaatga
     2401 cccccacatt tttgatggcc tgaatttgtg ccggggcatg cctggcaatg tcgacaaagc
     2461 actgctttgc aatcaaaagg tctccccgcc tgtaactttt taatcccatg agttcaacaa
     2521 cctccaaatc gaaaaccccc gtatcaaagg cgctctgcaa acagacctct gcctgatcag
     2581 ttatttgttt atccctgaat cgatggtaca attccttgta cgtaggggcc gcctcatcgg
     2641 aatccaattg caatgaacag gccttatcaa gcgccgcttt ggcactccag gggtcat
//
LOCUS       DATDUN010000375         3218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_308915, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000375 DATDUN010000000
VERSION     DATDUN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308915"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VJC18_05240"
     CDS             <1..391
                     /locus_tag="VJC18_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLD44820.1"
                     /translation="EVKSYLEVLAEKGMTPVSKKEDAVVVHDSCVYARHEGVVDEPRQ
                     LLERAGVTIKNPRECKKLTFCCGGPAESLFPSKAFAVGAKRMKQLSKEGNKVAVMCPI
                     CYANLKHAANKDVQLVDMADYLGECYL"
     gene            430..2151
                     /locus_tag="VJC18_05245"
     CDS             430..2151
                     /locus_tag="VJC18_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HLD44821.1"
                     /translation="MENPRIGVYICWCGSNIAKMVDVEAVSAAMAKLPNVVIAKNYKY
                     MCSDPGQELIVRDIKENNLTRVVVAACSPRMHEPTFRRALANAGLNPYLFEMANIREH
                     DSWVHEDRAQATEKALDLVSAAISRVAYHEVLDSRTVSINPATMVVGGGIAGMSAALE
                     IANAGKKVFLVERTNKLGGLMSHLDGAYQDFHDISRSLNVKVEQVQKHPQISVYLNSE
                     LKDVFGYIGNFEAKITQQGKDVDLKFGNIIMATGLKTFDPTVIPEYGYGKLANVVTSV
                     DFERMLKNGKVHTKAGQTPKSVAIIHCVGSRNEKYHEYCSRNCCMTALKYSVQLKKAL
                     PKTRVYDLYADMRAFGKCGEDLYRQASENKVLFMMFDQQEGLPLISQSQEGDGAGLTI
                     TINDKLSQETLEVPADMVVLMVGVEAQADAKQMSHFVGISLCGHDFFIEKHPKLDPVA
                     TTTSGVYIVGSCQGPKDIPDSVSQARAASARVLGTICKGNVAVEVTTACVNEELCCGC
                     QTCVTVCPYSAVSFNAEKKVSVVNEVLCKGCGTCGAGCPSGAIRCRHFTDEQIMSQIE
                     GLLGFVA"
     gene            2260..2748
                     /locus_tag="VJC18_05250"
     CDS             2260..2748
                     /locus_tag="VJC18_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HLD44822.1"
                     /translation="MTFEPKIVGFLCNWCSYTGADLAGTSRIKYPANIRVIRVMCSGR
                     VDPSFVLKAFREGADGVLICGCHPGDCHYHEGNYKCLRRFHLLKKYVEQMGIHPDRLR
                     LDWISASEGKQFAELIQNMTATVRNLGPSTVKKTLDACEKSSHAHCQDNAGVKKDIKQ
                     TA"
     gene            2837..>3218
                     /locus_tag="VJC18_05255"
     CDS             2837..>3218
                     /locus_tag="VJC18_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HLD44823.1"
                     /translation="MTKPKIKMAVYWGAACGGCCVSVLDVHEKLFTVVEHADIVFWPI
                     ALDFKYKDVEAMPDKSIDLCLFNGAVRNSENEHMACTLRKKSKVLVAYGSCAHLGGIP
                     GLANFKTKEGIFNRAYLESESTVNP"
BASE COUNT          788 a          713 c          927 g          790 t
ORIGIN      
        1 ggaggtcaaa agttatctgg aggttttggc cgaaaaagga atgacaccgg tatccaaaaa
       61 agaggatgcc gtggtggtgc atgattcatg cgtgtatgcg cgtcatgaag gagtggtgga
      121 tgaaccacgc cagttgctgg aacgcgccgg tgtgaccatc aaaaatccgc gtgaatgtaa
      181 aaaactcacc ttctgttgtg gggggcctgc ggaatcgctg tttcccagca aggcgtttgc
      241 ggtgggtgca aagagaatga aacagttgtc gaaagagggc aacaaggttg cggtgatgtg
      301 tcccatttgt tacgccaatc tcaaacatgc ggccaacaaa gatgttcagt tagtggatat
      361 ggcggattat ctgggtgagt gttatttgta ggggcaggtt ctaaacctgc ccgtacgcgg
      421 agataaatta tggaaaatcc aagaattggt gtttacattt gttggtgcgg ttcaaacatc
      481 gccaagatgg ttgatgtgga ggctgtgtcc gcggccatgg ccaaattgcc caacgtggtc
      541 atcgcaaaaa attacaagta catgtgttcc gatcccgggc aggaacttat cgtccgcgat
      601 atcaaagaga ataacctcac gcgcgtggtg gtggcagcgt gttctccccg catgcacgaa
      661 cccacttttc gcagggcgct ggctaatgcg ggactcaatc cttacctgtt cgaaatggcc
      721 aacattcgcg aacacgattc ctgggtgcac gaggaccggg ctcaagccac tgagaaggcg
      781 ctcgatcttg tgagtgccgc catcagtcgt gtggcgtatc acgaagtgct cgatagccgc
      841 accgtatcga tcaatcccgc aacaatggtg gtcggtggtg gtattgctgg catgtctgcg
      901 gcgctggaga ttgccaacgc gggaaaaaag gtttttctgg tggaacgcac aaacaagctc
      961 ggtggcctca tgtctcatct ggatggcgcc tatcaggatt ttcatgacat cagtcgatcg
     1021 cttaacgtca aagtcgaaca ggtgcaaaag cacccgcaga tctctgtgta cctcaattct
     1081 gaactcaagg atgtttttgg ttacattggc aactttgaag ccaaaatcac acagcagggc
     1141 aaggatgttg acctcaaatt tggtaacatc atcatggcta ctggtcttaa aaccttcgat
     1201 cccaccgtga ttcccgagta cggttatgga aaattggcca atgtggtaac ctcggtggat
     1261 tttgaacgca tgctcaagaa tggcaaggtg cataccaaag cagggcagac cccgaaaagt
     1321 gtggcgatca ttcactgtgt gggtagccgc aacgaaaaat atcacgagta ctgttcacgc
     1381 aattgctgca tgactgcgct taaatattcg gtgcaactca aaaaggccct gcccaagacg
     1441 cgcgtttacg atctctatgc cgatatgcgg gcctttggaa aatgtggcga agatctttac
     1501 cggcaggcct cggagaacaa ggtgttgttc atgatgtttg accagcaaga gggtcttccg
     1561 ctgatcagtc agtcccagga gggtgatggt gccggtctga caatcacgat caatgataaa
     1621 ctgtctcagg aaaccctcga ggtcccggca gacatggttg ttttgatggt gggtgttgag
     1681 gcgcaggccg atgccaagca gatgtcgcac tttgtgggca tcagtctttg cggacatgat
     1741 ttttttatcg agaaacaccc caaactcgat cccgtggcca ccactaccag tggcgtgtac
     1801 attgtgggtt cttgtcaggg accaaaggat attcccgata gtgtgagtca ggcgcgggcg
     1861 gcgtcggcgc gagtgttggg aacgatttgt aagggtaacg tggcggtcga agtgaccacg
     1921 gcgtgtgtaa acgaggagct ttgctgtggt tgtcagactt gcgtcacggt gtgtccgtat
     1981 tcggcagtga gtttcaatgc agagaaaaag gtttcggtgg tgaacgaagt tttgtgcaag
     2041 ggttgcggca cgtgcggggc agggtgtccc agcggcgcga ttcgttgccg tcattttact
     2101 gatgagcaga tcatgtcgca gatcgaaggg ttgttgggat ttgtggcata attcgtgaat
     2161 cgtgaatcgt gaatcgtgaa ttgtgaattg tggatcgata cagaccacaa ttttcgaaca
     2221 acgatcaacg atctacgatc cacgattttg gagcaattta tgacattcga accaaaaatt
     2281 gtgggttttt tgtgcaactg gtgctcctat acaggggccg atttggcggg aacgtctcgc
     2341 atcaaatacc cggccaacat tcgcgtgatt cgcgtgatgt gttctggccg tgtggatccg
     2401 tcgtttgtgc tcaaggcgtt ccgcgaaggg gcagatggag tgctcatttg cgggtgtcac
     2461 cccggtgatt gccattatca cgaaggcaat tacaaatgtt tgcgccgttt tcaccttttg
     2521 aaaaaatacg tggagcagat ggggatccat cccgatcgac tgcggctcga ttggatcagt
     2581 gcctccgagg gcaaacagtt tgccgagctg attcagaaca tgaccgctac ggtgcgcaac
     2641 ctgggcccaa gcacggtcaa aaagacattg gacgcgtgcg aaaaatcgtc tcacgcgcat
     2701 tgtcaggaca atgcaggtgt aaagaaggac attaagcaga cggcttagaa cgagcgcacg
     2761 agcgcatgaa ctcacgagcg cacgatgaat tttcgtgagt tcgtgagttt gtgagttcgt
     2821 gagttcggtg aaggatatga ccaaaccaaa aatcaaaatg gcagtctact ggggggccgc
     2881 atgtggcggt tgttgcgtgt cggtactcga cgtgcacgaa aagcttttca ctgtggttga
     2941 acacgcggat attgtgtttt ggcccattgc gcttgatttc aagtacaagg atgttgaggc
     3001 catgcctgac aaatccattg atctttgctt attcaatggg gcagtgcgca acagcgaaaa
     3061 tgagcacatg gcctgtacct tgcgtaaaaa atccaaggtg ttggtggcct atggttcttg
     3121 tgcgcatttg ggcggtatcc cgggccttgc caatttcaag accaaagagg ggattttcaa
     3181 ccgagcctat cttgaatcag aatccaccgt gaatcctg
//
LOCUS       DATDUN010000376         3684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_557603, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000376 DATDUN010000000
VERSION     DATDUN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557603"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /gene="ftsZ"
                     /locus_tag="VJC18_05260"
     CDS             complement(<1..988)
                     /gene="ftsZ"
                     /locus_tag="VJC18_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HLD44824.1"
                     /translation="MFEFEDDLSVAAKIKVVGVGGGGGNAIKTMMRARMEGIDFAAIN
                     TDIQALKLNEAPTKIQIGSQLTKGLGAGANPEIGRHAAIEDAAIIQETLKGSDMVFIT
                     AGMGGGTGTGAAPVIGKLARELGILTVGVVTKPFSFEGKKRMRHADEGIAALKNEVDT
                     LICIPNDNLLNLADKNTPIIDSFKMVDHVLLQAVKAISELITTPGLINLDFADVNTIM
                     RNAGVAVMGTGVASGENRALEAARLAISSPLLENISVAGATGLLLNITGSSNMTLFEL
                     NEACKLIQQEAHEDANIIFGSVIDDNAGDEISVTVIATGFDQEQLDTLTDEHS"
     gene            complement(998..2218)
                     /gene="ftsA"
                     /locus_tag="VJC18_05265"
     CDS             complement(998..2218)
                     /gene="ftsA"
                     /locus_tag="VJC18_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HLD44825.1"
                     /translation="MAKKENLIVGLDIGTTKICAIVGEVTHDGIDVIGIGSHPSRGLR
                     KGVIVNIEATIDSMKKAIEEAELMAGCDILSVYAAISGSHIKGINSHSIVSVKNKLIT
                     DRDIERVIDGASAVVMTMDRESIHVIPQEFIVDDQDGIKEPLGMAGSKFEANVHIVTA
                     STACIQNILKCATRCGVHVNDIILGPLASSYASLTEDEKELGVIVLDAGGGTTDLVIY
                     VNGSVVHTASIPLGGNHITNDIAVGLRTPALEAEKIKQKYGCALASMINKNDTIDVPS
                     VGGRKPRILSRQILAEIIEPRVEEIFDLVRQEIVRSGYEDNIASGVVLTGGMANLEGT
                     TEMAEQIFDFPVVRATPRGVGGLVDVVNSPIYTTGVGLVLCGYHKKQQLQPKNENVYV
                     KVKKKMGTWLGDVF"
     gene            complement(2227..3084)
                     /locus_tag="VJC18_05270"
     CDS             complement(2227..3084)
                     /locus_tag="VJC18_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HLD44826.1"
                     /translation="MLLGTNNKNRKWQNRPLLYKGRYYYIRRSLQWLLVVCVLSGVTG
                     LFFYFKNSDALCLQNITVSGDLNHLTRDDVVTLSELTPGDKLFESTLNVLRKKIARHP
                     WVADVKIRRKFPDTIQIHVTEKKAKALLLANKLYLIDEEGRVFKPLEKNEPADLPIMT
                     GFREDATQQHPHLTKDYFQQTLTLLDFLQQQDFYNEDSLSELHFDPVYGFTVYTKNRA
                     LEIYYGRDSFVQKQQTLEKFKLSDHYHNERLTRLDLDTPGRVIARQTALPASLPAATV
                     QGNNSNSLR"
     gene            complement(3075..>3684)
                     /gene="murB"
                     /locus_tag="VJC18_05275"
     CDS             complement(3075..>3684)
                     /gene="murB"
                     /locus_tag="VJC18_05275"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HLD44827.1"
                     /translation="KLCQEEGIADIMSLTGIPGSVGGLVKMNAGIPSREIKDVVRNIT
                     IITKEGEQQTLSREHLDFEYRSLKLPRTHFILSVLFRFRDLSSPEDALAIMRQHQEAR
                     VSKQPLEFPSLGSVFKNPQPLHRKDIATPAGKLIEEAGLKNIRIGGARISPKHANFII
                     NENEAKASDVLSLISLIKDKVKQISQIELETEIRILGEEACC"
BASE COUNT          753 a          949 c          998 g          984 t
ORIGIN      
        1 gagaatgctc atcggtgagt gtgtccagtt gttcttgatc gaaacccgtg gcaatcacgg
       61 tgactgagat ttcgtcgccg gcattgtcgt cgatgacgga accaaaaatg atgttggcgt
      121 cttcgtgtgc ctcttgttgt atcagtttgc aagcttcgtt gagctcgaat aaggtcatgt
      181 ttgaagaacc tgtgatattc aagaggagtc ctgtggcccc cgcgaccgat atgttttcga
      241 gcaggggaga agagatggcc aaacgcgcgg cctccagagc acggttttct cctgaggcga
      301 cccctgtgcc catgacggcc acacccgcgt tgcgcatgat ggtgttgacg tcggcgaaat
      361 ccagattgat gagcccgggc gtggtgatga gctcggaaat ggccttcacg gcttgcagga
      421 ggacatggtc caccattttg aaactgtcga tgatgggggt gtttttgtct gccaagttga
      481 gcagattatc attggggata cagatgagcg tgtccacttc attcttgaga gcggcgatcc
      541 cttcgtctgc atgccgcatt cgtttctttc cttcgaaaga gaagggcttg gtcaccacgc
      601 ctactgtgag gatacccaat tcgcgggcga gcttgccaat cacgggagcg gcccctgttc
      661 ctgtgccacc gcccatgccg gctgtgatga aaaccatgtc tgatcccttg agagtttctt
      721 gaatgatggc cgcgtcttct atagcggcat gtcgtcctat ctcaggattg gcgcctgctc
      781 ccagtccctt ggtgagctga gacccgatct ggattttggt gggcgcctca ttgagtttga
      841 gagcctggat atcggtgttg atggcggcga agtcgatgcc ctccatccgg gccctcatca
      901 tggtcttgat ggcgttaccg ccgccgccgc ccacacccac aacttttatc ttggcggcca
      961 cactcaaatc gtcttcaaat tcgaacataa gagctcctta aaaaacgtca cccaaccagg
     1021 tacccatttt tttcttgacc ttcacgtaca cgttttcgtt tttgggctgg agctgttgtt
     1081 ttttgtgata cccacaaagc accagaccaa cacctgtcgt gtagatgggg ctgttcacga
     1141 catccacgag accaccgacc ccgcgggggg tcgctcgcac aaccgggaaa tcaaaaatct
     1201 gttcggccat ctcggttgtt ccctccagat tggccatgcc ccccgtcaga accacaccgg
     1261 aggcgatgtt gtcttcgtaa cctgagcgca cgatttcctg acgaaccaga tcgaaaattt
     1321 cttccacgcg cggttcgatg atttcggcca gaatctgtcg tgataaaatt ctgggtttgc
     1381 gaccgccgac ggaagggacg tcaatcgtgt catttttgtt aatcatgctg gccagggcac
     1441 agccatactt ttgtttgatt ttttcggctt ccaacgccgg tgttctcagg ccaacagcga
     1501 tgtcgttggt gatgtgattg ccgcccaggg ggatcgaggc tgtgtgcacc actgacccgt
     1561 tgacgtagat gaccaggtcg gtcgtgccgc cgcccgcatc cagcacgatc acgccgagtt
     1621 ctttttcgtc ttcagtcagg gaggcgtagg aactggcgag tgggcccaaa atgatgtcgt
     1681 tcacatgaac gccgcaccgt gtggcacact tcagaatatt ctgaatgcag gcagtcgaag
     1741 cggtcacgat gtgcacgttg gcctcgaact ttgaccctgc catgcccaga ggttccttga
     1801 tgccgtcctg atcatccacg atgaattcct gcggaatcac atggatgctt tcgcggtcca
     1861 tggtcatgac cacggctgac gcgccgtcaa tgacgcgttc gatatcacgg tcggtgatga
     1921 gcttgttctt gaccgacacg atgctgtggc tgttgatccc cttgatgtgg cttcccgaga
     1981 tggcagcgta caccgacaga atatcacaac cggccatgag ttcggcctct tcaatcgctt
     2041 ttttcatgga gtcaatggtg gcttcgatgt tgacgatgac ccctttgcgc aaacctcggg
     2101 aggggtggga gccgatccca atgacgtcga tcccatcgtg tgtgacctca ccaacaatgg
     2161 cgcagatctt tgtggtgccg atgtcgagtc ccacaattaa attttctttt tttgccataa
     2221 gcgtcctcaa cgtaatgaat tactattgtt cccctgaact gttgccgccg gaagagatgc
     2281 tggtagtgcc gtttgtctgg cgatcactct tcctggcgtg tccaagtcca atcgagttag
     2341 tctctcgttg tgataatggt ctgacaattt gaatttttcc aaggtctgtt gcttttgtac
     2401 aaagctgtct cttccgtaat agatttccaa agccctgttt ttggtgtaca ctgtaaagcc
     2461 gtacacggga tcgaaatgaa gctccgacaa actgtcttca ttataaaagt cttgctgttg
     2521 taaaaaatcc agcagggtca gcgtttgttg aaaataatct tttgtgaggt gtgggtgctg
     2581 ctgggtggcg tcttccctga aacctgtcat gataggaagg tcagcgggtt cattcttttc
     2641 cagcggcttg aacacgcgac cctcctcgtc gatgaggtag agcttgttgg ccagcaacaa
     2701 cgccttcgct tttttctccg tgacatgaat ctggatggtg tcgggaaatt tgcggcggat
     2761 tttcacatcg gccacccagg ggtggcgtgc aatcttcttt cgcaaaacat tcagggtgct
     2821 ttcaaacaat ttgtctcccg gagtgagctc tgagagggtg acgacatcgt cccgggtcag
     2881 atggttgagg tcgccggaga cggtgatgtt ctgcaaacag agcgcgtcgg agtttttgaa
     2941 ataaaagaac agccccgtaa cgccagacaa gacgcacacg accaacaacc actggaggga
     3001 acggcggatg tagtaatatc gccccttgta tagcaagggc ctgttttgcc attttctgtt
     3061 tttgttgttg gttcctagca acatgcctct tctcccaaaa tcctgatctc agtttccagc
     3121 tcgatttggc tgatctgttt taccttgtct ttgatcagac tgatcaaaga cagcacatcg
     3181 gacgccttcg cctcattttc gttgatgatg aagttggcgt gcttgggtga aatgcgggcg
     3241 ccaccaatgc ggatgttttt gagccccgct tcctcaatga gtttgcccgc aggggtcgcg
     3301 atgtctttgc ggtgcagagg ttgtggattt ttgaacacag agcccagact gggaaactcc
     3361 agcggttgtt tgctcacccg ggcttcctga tgctgacgca tgatcgccaa cgcgtcttcc
     3421 ggtgatgaga gatccctgaa ccggaacagg acgctcaaga tgaagtgggt gcgtggcagt
     3481 ttgagtgagc ggtattcaaa atcgaggtgc tcgcgggaca gcgtctgttg ctctccttct
     3541 ttggtgatga tggtgatgtt tctcacgacg tccttgattt cgcgggaagg gatgcccgcg
     3601 ttcattttta cgagcccgcc cacactgccg ggaatccctg tgagggacat gatgtcagcg
     3661 atgccttctt cctggcacag cttg
//
LOCUS       DATDUN010000377         1133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_60034, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000377 DATDUN010000000
VERSION     DATDUN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60034"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VJC18_05280"
     CDS             <1..392
                     /locus_tag="VJC18_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008490648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLD44828.1"
                     /translation="SPGGDVVSGLAIYDTIQYIRPQVQTICIGQAHNIAALLLACGAK
                     GKRIALKNSNILLRQPMGGIGGQASDIEIQTRENVRIRKQMNEILSRHSGQTVEKITQ
                     DTDRDFFLSADEAKNYGLIDQTWESRP"
     gene            412..>1133
                     /gene="clpX"
                     /locus_tag="VJC18_05285"
     CDS             412..>1133
                     /gene="clpX"
                     /locus_tag="VJC18_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HLD44829.1"
                     /translation="MAKEQNLRCSFCGKSQEEVEKIIAGPTVYICDECIDLCNDIIEE
                     ERAKETEQTPQHHLLKPKQIKAILDQYVVGQQEAKKTLSVAVYNHYKKISAPHKTSDV
                     ELAKSNVLLIGPTGTGKTLLAQTLAKILKVPFAMADATTLTEAGYVGEDVENIILKLL
                     QASDYDIEQAQKGIIYIDEIDKITRKSENVSITRDVSGEGVQQALLKIIEGTQANIPP
                     QGGRKHPQQEFLQVDTSSILFI"
BASE COUNT          327 a          316 c          274 g          216 t
ORIGIN      
        1 acagtcccgg cggggatgta gtgtcaggtt tggcgattta tgatacaatc cagtatattc
       61 gcccccaggt gcagacgatt tgcattggtc aagcccacaa cattgccgcc ttgttgctgg
      121 cctgtggggc aaagggcaaa agaattgcat taaaaaattc caacatcctt cttcggcagc
      181 ccatgggcgg cattgggggg caggcatcgg acatcgaaat ccaaacccgc gaaaacgtgc
      241 ggattcggaa acagatgaac gaaatcctct cacgccacag cgggcaaacg gtggaaaaaa
      301 tcacccagga tacagatcgc gattttttcc tcagcgccga cgaagcaaag aactacgggc
      361 ttatcgatca aacttgggaa tctcggccct aaaacgctct ggaggcatca gatggctaag
      421 gaacaaaacc ttcgctgctc cttttgtggg aaatcccaag aggaagttga aaaaatcatc
      481 gccgggccta ccgtctacat ctgtgacgag tgcatcgacc tctgcaacga catcattgaa
      541 gaagaacgcg ccaaggagac cgaacaaact ccccagcacc atctgctaaa acccaagcag
      601 atcaaagcaa tccttgatca atatgtggtt ggtcagcaag aagcgaaaaa aacgctcagc
      661 gttgccgtct acaaccacta caaaaaaatc tctgcccccc acaagaccag cgatgtggaa
      721 ctggccaagt ccaacgtttt gctcatcggc cccacaggca caggcaaaac attgttggcc
      781 caaaccctgg ccaagatttt aaaggtgccc tttgccatgg ccgatgccac caccctcacc
      841 gaggcgggct acgtggggga agacgtggaa aacatcatcc tcaagctgtt gcaggcctct
      901 gattacgaca tcgaacaggc acaaaagggg atcatctata tcgacgagat cgacaaaatc
      961 acccgcaaat cagaaaatgt ctccatcacc cgcgacgttt ccggcgaggg cgtgcagcag
     1021 gcattgctga agatcattga aggcacccag gccaacattc ccccccaagg cgggcgtaag
     1081 cacccccagc aggaattttt gcaggtggat acctctagca tcctgttcat ttg
//
LOCUS       DATDUN010000378         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_517713, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000378 DATDUN010000000
VERSION     DATDUN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517713"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VJC18_05290"
     CDS             <1..996
                     /locus_tag="VJC18_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD44830.1"
                     /translation="MVLVGIFSSLFDICMKAAIPVMMGKADTLRANSILLGGRNFVVA
                     GSALCAVFAHVWMTRFDTIFVIDAVTYLISGFVLLTVNIKTSELPVSSQACAIGMVAK
                     PKPVRKNIFGEYAEIWRLRNAPILFLFMTVMFIDAFASASHNVGWPLFSKSYSPERPM
                     FLYGFVLFFWAGGNVLGIVFLNKLAMLKRLAPEVLYLFFTIMMSVGMILTFAFDAKLL
                     IAVAALIAGVGDGTYQTYFTTYVQKVDDAVRGKVFALTNIFLKTGFGLGFIVIPMVMD
                     SLGPAWTAALFHGLVVLCCGLGLWYPTSVMVRCFASKGQMLCVKGSNAERQRVRC"
     gene            complement(1016..1255)
                     /gene="acpP"
                     /locus_tag="VJC18_05295"
     CDS             complement(1016..1255)
                     /gene="acpP"
                     /locus_tag="VJC18_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLD44831.1"
                     /translation="MAKALLESKIIDIIAAQLSLRNSDIKLSSHFIDDLGADSLDVVE
                     LIMEIEDVFGIEIPDEDVEEMETVEDVINYVAKTK"
     gene            complement(1259..1441)
                     /gene="rpmF"
                     /locus_tag="VJC18_05300"
     CDS             complement(1259..1441)
                     /gene="rpmF"
                     /locus_tag="VJC18_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HLD44832.1"
                     /translation="MAVPKRRTSKSKKGMRRSHHHLRALDVSVCPNCQNAKLPHRVCP
                     HCGYYKSKEILTLDKE"
     gene            complement(1521..2096)
                     /locus_tag="VJC18_05305"
     CDS             complement(1521..2096)
                     /locus_tag="VJC18_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HLD44833.1"
                     /translation="MNKINIHHISVTKGLDLTLNQKDEWFLNLIKRLIPEHRLDPNSL
                     VGQFNFLRTNTIVDVMGSISFTHYPPCDRCATEIKSDENISFSTHLSPASLVEDKKKR
                     GAHGKTPDNETEIELHEDELDHCFYHNEEIDLEPLINDEVALSIRYNYYCTPAQDCKI
                     PVPNQEYITVNDTSDPRWAPLVKLKNTKPNS"
     gene            2187..2690
                     /gene="rpiB"
                     /locus_tag="VJC18_05310"
     CDS             2187..2690
                     /gene="rpiB"
                     /locus_tag="VJC18_05310"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HLD44834.1"
                     /translation="MSKERIAIGADHAGFLLKEEVKSYLIQLGYDVVDHGCKSEDRVD
                     YPDYAKLVAEDVGQGRVDRGLLVCGSGIGMAITANKFAGVRAASIIDEVSAKLSRQHN
                     DLNVLCLGARLLDWEHAKAILEIFLNTEFEGGRHAERVEKIRTHERMNSSPQSGIPLA
                     GRAHEGT"
     gene            2847..>3254
                     /locus_tag="VJC18_05315"
     CDS             2847..>3254
                     /locus_tag="VJC18_05315"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455119.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HLD44835.1"
                     /translation="MNFDLLQKQDAEVFSAVEKEIARQENKLEMIASENFVSKAVLEA
                     LGSPLTNKYAEGYPHKRYYGGCEYVDIVEDIAIERAKKLFGAEHANVQPHSGSQANMA
                     VYMSVLNPGDTFMGMNLSEGGHLTHGSPVNFSGR"
BASE COUNT          770 a          657 c          901 g          926 t
ORIGIN      
        1 atggtcctgg tggggatctt ttcgagtttg tttgatatct gcatgaaagc ggcgatcccg
       61 gtgatgatgg ggaaagcgga tacactgcgg gccaactcga ttcttttggg gggacgcaat
      121 tttgtcgttg ctggttccgc cttgtgtgct gtcttcgctc atgtctggat gaccagattt
      181 gataccatct tcgtgattga tgccgtcact tacctcatat ccggttttgt tttgctcacg
      241 gtcaatatca aaacgagtga gctccctgtg tcgtcgcagg cgtgcgcaat tgggatggtg
      301 gcaaagccca agcctgtgag aaaaaatatt tttggcgagt atgccgagat ttggcgtctg
      361 cgcaacgcgc ccattttgtt tttgttcatg acagtgatgt tcatcgatgc ctttgccagc
      421 gcatcccaca atgtgggctg gcccctgttt tccaagagct attcccctga gaggccaatg
      481 tttctgtatg ggtttgtgct gtttttttgg gcggggggca atgtgttggg gatcgttttc
      541 ttgaacaaac tggccatgct caaacggttg gctcccgaag tcttgtattt gttttttacg
      601 atcatgatgt ctgtggggat gatactgacc ttcgcgttcg atgccaagct gctgattgct
      661 gtggccgcct tgattgcggg tgtgggggat ggcacgtatc aaacatattt taccacatat
      721 gtgcaaaagg tcgatgatgc cgtgcgtggc aaggtttttg ctctgacgaa tatttttctc
      781 aaaaccggat ttggtctggg tttcattgtc attccaatgg tgatggactc tctgggaccc
      841 gcctggacag cggctctctt tcacgggttg gtagtgctgt gctgtgggct gggactgtgg
      901 taccccacgt cagtcatggt cagatgtttt gcgtcaaagg gtcaaatgct ttgcgtcaaa
      961 gggtcaaatg ctgagcgtca aagggtcaga tgctgagcgt caatgggtca atgagttatt
     1021 ttgtcttggc cacgtaattg atcacatcct cgacggtttc catttcttcg acatcttcgt
     1081 cgggaatttc aatgccaaag acatcctcga tttccatgat gagctcgaca acatcgaggc
     1141 tgtcggcgcc caaatcgtcg atgaaatgtg aactgagttt gatgtcgcta tttcgcagag
     1201 acaactgagc ggctatgatg tctatgattt tagactcaag taaagccttg gccacggatc
     1261 actccttgtc cagagtcagg atttctttgc ttttgtaata accacagtgg gggcagacgc
     1321 ggtgcggtag tttcgcgttt tgacaattgg gacacacaga cacatcgagg gcacgcaagt
     1381 ggtgatgtga acgacgcatg ccttttttgg atttcgatgt tcttctcttt gggacagcca
     1441 taaacaactc ctcgtttacc caaaaaaata aaaaatgggt cgccttaact agtatttaat
     1501 agactggcat gtcaagaaaa tcacgaattg ggcttggtgt tttttagttt taccaagggc
     1561 gcccaacggg gatcggacgt gtcattaaca gtgatatatt cttgatttgg cactggaatt
     1621 ttacagtctt gagctggggt acagtagtag ttgtatctga ttgacaaagc cacttcgtcg
     1681 ttgatgaggg gttccagatc gatttcttcg ttatgataaa aacaatggtc taactcgtcc
     1741 tcgtgtaact cgatttctgt ctcattatcg ggtgtttttc cgtgagcccc cctttttttc
     1801 ttatcctcaa cgagtgaggc tggggagagg tgagtcgaga atgagatgtt ttcgtccgat
     1861 ttgatttctg tagcgcaacg gtcgcaaggt gggtaatgtg taaaactgat ggagcccatg
     1921 acatcgacga tggtgtttgt cctcaaaaag ttgaattgtc ctaccaaaga gttgggatcg
     1981 agacggtgtt cggggatgag tctcttgatg aggttaagaa accactcatc tttctggttg
     2041 agagtgaggt cgagtccttt ggtgacggaa atgtgatgaa tgttgatttt attcacgtgg
     2101 ccgccactat caacaataca ggaaaagggc aagtgtggtt caactttgta ctttcttttt
     2161 ttgagttgat tcactatcaa tcgcccatgt caaaagaacg tattgccata ggggcggatc
     2221 acgcggggtt tttgcttaaa gaggaggtca aatcctacct gatccagttg gggtacgatg
     2281 ttgtcgatca tggttgcaag agtgaggata gggtcgatta tcccgattat gccaagttgg
     2341 tggccgagga tgtggggcaa ggtcgtgttg atagaggttt attggtgtgc gggtccggca
     2401 ttgggatggc gatcacggcc aataagtttg cgggtgtgcg tgccgccagc atcattgatg
     2461 aggtatcggc aaagttgtcc cgacagcaca atgacctgaa tgtgttgtgt ttgggcgcgc
     2521 gtctgttgga ttgggaacac gccaaagcta ttttggaaat ctttctgaat acggaattcg
     2581 aaggcgggag acatgcggag agggtggaga agatacgaac tcacgagcgc atgaactcat
     2641 caccgcagag cgggatcccg ttggcgggac gagcgcacga ggggacgtag gtcgaaaaca
     2701 cgagggatga aacaattgag caatttgcaa tgggcaatcg tagcaattgc ggcaattgca
     2761 atgattgcaa caattgctgt aattgctata gttgatgacg atccacgatc cacgatccac
     2821 gatccacgag taaggagtta acaaacatga actttgattt attacaaaaa caggatgctg
     2881 aagttttctc tgccgtcgaa aaagaaatcg cgcgacaaga aaacaaactc gagatgatag
     2941 cgagtgaaaa tttcgtttct aaagccgtac tcgaagcgtt gggatcacca ctgaccaata
     3001 agtacgccga agggtacccc cataaacgat attacggagg ctgtgagtat gtagacattg
     3061 ttgaggacat tgcgatcgaa agagctaaaa aattatttgg ggccgagcat gccaacgtgc
     3121 aaccccacag tggttcgcag gccaacatgg ctgtttacat gagtgtgttg aatccgggcg
     3181 ataccttcat ggggatgaat ttgtctgaag gtgggcatct cacacacgga tcgccggtta
     3241 atttttcggg tcga
//
LOCUS       DATDUN010000379         2591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_225526, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000379 DATDUN010000000
VERSION     DATDUN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225526"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..337)
                     /locus_tag="VJC18_05320"
     CDS             complement(131..337)
                     /locus_tag="VJC18_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44836.1"
                     /translation="MNSYSQIPIIVYKKHQREFTLTPTMRLQIRINQPHQLLSNFRDI
                     RGHLDTTFIEVGVQGEQGKISVLR"
     gene            380..>2591
                     /locus_tag="VJC18_05325"
     CDS             380..>2591
                     /locus_tag="VJC18_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44837.1"
                     /translation="MRSPQDCVSLLAVIDKLRQADLGIQWGAAESILQVVANACLNNA
                     VFGKFEAGQFDLDTFYLTMSQIAQQLVLLYHERGGLQYTEDELMHRLNEFLREYSHTT
                     GERALQQTISELIESKTPPQSEGEKLSALVLLFWEQPELVCLIEEYRLLLATRLTPQG
                     PNVLAPWPRFMDFCQRLIRFFYGYCAEKSLTESSRLLSDLFTGFWYEIDASLLANLSA
                     NFSFRKTERQYQVRGMLAIAGYSDLLHSTPWAKRKRSDIAQKVQVLFYSMVIECIMEK
                     PEVKGLSTFQQAALLRELLLYYDCYRDQQQSTFLGAGVAANRESAVLPIYTASAKQLI
                     QWGRATNIADLVWFYELIQTASVRHSGQRVEAENFCSLQSVVQDQTDILTEIIVRRLA
                     DLDAPEHLTSLQEWTRAGLPVRDLLARWRFKRADYSEPEPNSEYRERAVHRRKETAEL
                     VLNLMKALPTYKRLGQQGELLEIVTLLEANISLREAMQRQTLEHLWKKAGTFEARLAL
                     AFEHPFSRREMTLKRRLIEEEMGSEVEVQAVAGRIRQNFDDLYGEGDEVLAVEQIRYF
                     FGKRYSFETLNILLHTHSSDHNLKNTISSILDEDGFLVGAPKGEEVVVRLRHELHSID
                     AMLDQLYSLPLPIRLYVIKKFLIDGKLIVEPKERQRLYKLFLDETIVASEQNSAFATV
                     MRNVAQAMAATTIWEPIFHAITPTLAEHIARPPQAQTPWTSFGFPHMAQRAELID"
BASE COUNT          658 a          580 c          677 g          676 t
ORIGIN      
        1 gagggcctgg caacaagggt atgcgaatca ggggcacctt aattatttcc aaggttatcg
       61 tttactttat cgtgacgaaa gacagagggc aagggcccat caaaagcggt tggaaggcct
      121 tggccagctt tcagcgcaag acagaaatct tgccctgttc tccctgcaca cccacttcaa
      181 tgaatgtggt gtccagatgc cccctgatgt cgcggaagtt gctcaacagc tgatgaggtt
      241 gattgatgcg gatctgcaga cgcattgtgg gggtcagcgt gaactctctc tgatgcttct
      301 tgtacactat tatgggaatc tgggaatacg agttcaatcc cctcctttcg agagggtttt
      361 caagcagttt tgcaacgtca tgcgatcacc tcaagactgt gtctcattgt tggcagtcat
      421 tgataagctg aggcaggccg atcttggtat tcaatggggt gcggcggaga gtattttaca
      481 ggtggtagcc aatgcgtgtc tcaataatgc cgtatttggc aaatttgagg cagggcaatt
      541 tgatcttgac accttttatt tgaccatgag ccaaatcgcc cagcaattgg ttttgctgta
      601 tcacgagagg ggcggccttc aatacaccga agatgagctg atgcaccgcc ttaatgaatt
      661 cttgagagaa tattcccaca caacagggga gcgggcgtta cagcaaacaa tctctgagct
      721 tattgaatca aagacaccac cacaatctga gggagaaaaa ctctctgctc tcgtcttgtt
      781 attctgggag caaccagaac tggtctgtct tatcgaagag tatcgattgc tcttggccac
      841 acgcttgact ccccagggtc caaatgtgct tgccccctgg ccgcggttta tggacttttg
      901 ccagagatta ataagatttt tttatggcta ttgtgcagaa aaaagtctga cagagtccag
      961 tagactcctg agtgaccttt ttaccgggtt ttggtacgag attgatgcta gtctcctcgc
     1021 taatctctct gcaaatttct ccttccggaa gactgaacgt caataccagg tacgggggat
     1081 gctcgcaatc gctggttaca gcgatctgct acatagcact ccttgggcga agaggaaacg
     1141 ctcagacatt gcccagaaag ttcaagttct cttttactcg atggtgattg agtgcatcat
     1201 ggagaaacct gaggtgaaag gcctcagcac ttttcaacag gcggcgttgt tgagagaatt
     1261 gctgttgtat tatgattgtt atcgtgatca gcaacagtcc acgtttttgg gggcaggcgt
     1321 tgctgccaac agggaatccg ctgtcttgcc gatctacaca gcttctgcga aacagcttat
     1381 tcagtggggc cgcgcaacaa acattgcgga tctggtctgg ttttatgagt tgatccaaac
     1441 agcatctgtt cgtcacagtg gccagagggt agaggctgaa aacttctgtt ctcttcaatc
     1501 cgttgtgcag gatcaaacag atatcctgac cgaaatcatt gtcagacgtt tggcagactt
     1561 ggatgccccc gagcacctca ctagtttgca ggaatggacg cgtgcggggc ttcctgtaag
     1621 ggacctgttg gcgcggtggc ggttcaaaag agctgattat tcagaacctg agcccaatag
     1681 tgaatatcgt gagcgcgctg tgcacagaag aaaagagact gctgaactcg tgctgaacct
     1741 catgaaagcc ttgccaacct acaagaggct tgggcaacag ggtgagcttc tcgaaatcgt
     1801 gaccctcctg gaggcgaaca tttcgttgcg agaggccatg caaaggcaga cactggaaca
     1861 tctatggaaa aaggcaggga catttgaagc tcggttggcc ttggctttcg agcacccatt
     1921 ttctcggcgt gagatgacgc tcaagaggcg gttgatcgag gaagagatgg ggtcggaagt
     1981 cgaagtccag gctgtggcgg ggcggatcag acaaaacttt gatgatcttt atggtgaggg
     2041 tgatgaggtt cttgccgtgg agcaaatacg ttattttttt ggtaaaagat atagttttga
     2101 aactttgaat attctgttgc acacccattc atctgaccat aatttgaaaa acaccatcag
     2161 ctcgattctt gatgaagatg gttttctcgt gggtgctccc aagggtgaag aagtggttgt
     2221 gcgactgcgt catgagcttc acagtataga tgccatgctg gaccaactgt attcattacc
     2281 cttgccaatc agactgtatg tgatcaaaaa atttctgatc gatggaaaac tgattgtgga
     2341 accaaaagag cgacagagac tttataagct atttttggac gaaacaattg ttgcgtctga
     2401 gcaaaactca gcctttgcta ctgtgatgag aaatgtcgcg caggctatgg cggcgaccac
     2461 gatttgggag ccgatctttc acgccattac cccaactttg gccgaacaca ttgcccggcc
     2521 gccacaagca caaacaccat ggacaagttt cggatttcca catatggcac aacgggcaga
     2581 acttatagat t
//
LOCUS       DATDUN010000380         1510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_434861, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000380 DATDUN010000000
VERSION     DATDUN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434861"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="VJC18_05330"
     CDS             <1..170
                     /locus_tag="VJC18_05330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596897.1"
                     /note="The point of entry for the majority of electrons
                     that traverse the respiratory chain eventually resulting
                     in the reduction of oxygen; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HLD44838.1"
                     /translation="FQHSYSVVKGVDKILPVDIYVPGCPPRPEALTEGLLKIQEKMKT
                     EKQLVRHSKNT"
     gene            167..652
                     /locus_tag="VJC18_05335"
     CDS             167..652
                     /locus_tag="VJC18_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HLD44839.1"
                     /translation="MNQKEKEIASHLIDKFSKEVLDWCEPEKGDWYLFVPASKLVEIL
                     LYLKDTWEFCFDHLVNLSAFDAQEHIEIVYHLYSYRHKQRFTLKVRTSRLQGEVPTVS
                     TVYPTADFQEREVYDLLGVAFAGHPHLTRIMLPEDWVGHPLLKDYEEGAEYGGIGTGR
                     K"
     gene            854..>1510
                     /locus_tag="VJC18_05340"
     CDS             854..>1510
                     /locus_tag="VJC18_05340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985600.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HLD44840.1"
                     /translation="MTQTETHNDSFHTEEMVVNMGPQHPSTHGVLRFIVHTDGEVMRK
                     AVPDIGYLHRGIEKIAEKVGYAGFMPYTDRIDYVAAMTAGQGYALAVEKLMGIEVPKR
                     AEYLRVVACELNRISSHLVAAGSLGLDMGASTPFVHALREREAINDLLESLCGARLTY
                     NYMRIGGVSFDLPKGFEEKTVLFLNHFEPIIDEYNRLLTDNKIFLKRLSGVAVISKED
                     A"
BASE COUNT          394 a          356 c          427 g          333 t
ORIGIN      
        1 tgttccaaca cagctattcc gtggtcaaag gggtcgataa aatcctgccc gtcgatattt
       61 acgtcccggg ctgtcctccc agaccagagg cgctcacaga aggattactc aagattcagg
      121 aaaaaatgaa aactgagaaa caattggtca ggcatagtaa gaacacatga accaaaaaga
      181 aaaagaaatc gccagtcacc tcatcgataa attttccaaa gaagtgctgg actggtgtga
      241 gccggaaaag ggtgattggt atctgtttgt tcctgccagc aagcttgttg aaatactgct
      301 ttatctcaaa gacacgtggg agttttgttt tgatcatctg gtgaatctga gcgcgttcga
      361 tgcccaggaa cacattgaga ttgtgtatca cctgtactca tacagacaca aacagcgctt
      421 cacgctcaaa gtgcgaacca gccgcctgca gggcgaggtc cccacggtga gtacggttta
      481 tcccacggcc gattttcagg aacgcgaggt atatgatttg ttgggagtgg cctttgcagg
      541 gcatccccat ttgacccgca tcatgttgcc cgaggactgg gtggggcacc cgctacttaa
      601 ggattatgag gaaggggccg agtatggggg gattgggacg gggaggaaat agaccaaagt
      661 ccaaagacta aagttggggg gcgacccgcc tgcgccgcag actatggcgc ggcaagggga
      721 cgaaaacacg agggacgatt gtcatccctg cgaaggcagg gatccagaat tgttgaaaat
      781 atggattcca gcgtgcgctg gaatgacaaa cgatacgcga tacgcgatac gcttttggct
      841 ttacgaaaga tagatgactc aaaccgaaac acataatgac tcttttcata ccgaggaaat
      901 ggtggtcaac atggggccac aacacccgtc gacccacggg gtgttgcgct ttatcgtgca
      961 caccgatggc gaggtcatgc gcaaagccgt gcccgatatt gggtacctgc atcgcgggat
     1021 cgaaaaaatc gccgaaaagg tcgggtacgc aggattcatg ccctacacgg accgcattga
     1081 ttacgtggcc gcgatgacag ccgggcaggg ttatgccctg gctgtggaaa aattgatggg
     1141 gatcgaagtc cccaaacgcg ccgagtatct gcgtgtggtg gcctgcgaac tcaaccgcat
     1201 ttcttcgcat ctggtggcgg cagggtcgct gggactcgat atgggtgcgt ccacgccgtt
     1261 cgtgcatgcc ctgcgggaac gcgaagccat caacgatttg ctggagagtc tctgtggggc
     1321 cagactcact tataactaca tgcggatcgg cggtgtttcg ttcgatctgc ccaaaggctt
     1381 cgaagaaaaa acggttttgt ttttaaatca ttttgaaccg atcatcgatg aatacaatcg
     1441 tttgctcact gataataaaa tctttctcaa acggttgagc ggcgtggccg tgatcagcaa
     1501 agaggacgcg
//
LOCUS       DATDUN010000381         3247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_434875, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000381 DATDUN010000000
VERSION     DATDUN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434875"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1463)
                     /locus_tag="VJC18_05345"
     CDS             complement(99..1463)
                     /locus_tag="VJC18_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLD44841.1"
                     /translation="MSPAQALTDKARLHDVKFLLNTLEQNYAYTWLKRVDWDALREKY
                     IPLVRSHTTDQKHYELLTNILQELRDSHCLIINPTLPERFNPGFVINEIENKYIVSEV
                     SVNSPAYRSGIRKGFELTRVQHQPVAQVLNDFQKHLMGSPQWIRAYSTDLLATVPGTS
                     KKTYTPQTFTFFDQQRKEKDIFLSVPAGVELTERSFWRWESSRPLVQSTTLPGNVGYI
                     KVTGFKHKKGQEPSVSSQFRNHLRGLSQRQTKGIVIDLRNTNGGYGDEVCRMASFFMP
                     DQTLLRQTCHPKSRNCIVQNKSKLRLSMNYTTYPDLYMYGGPVVLLTNSYCRSACDMF
                     SAAFKMTGRGRIIGQTTAGAGGTYSCARLPSKVPFCYTNWPVVLADGSLVEGVGISPD
                     VPLRQTLKDLQRGQDTLLTAAKSHIEKQFRIAIELEKLNSRRQTFDDALFAEPARRQT
                     SGYR"
     gene            complement(1583..>3247)
                     /locus_tag="VJC18_05350"
     CDS             complement(1583..>3247)
                     /locus_tag="VJC18_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44842.1"
                     /translation="EINARTFPGDANSRPVITNNPPVANAGPDQTVVINSKVALDGTD
                     SAGEDLIYSWSIESGYATFSDSTAQAPTLTTYYSQGDVVAKLTVTDINNESASSEVTV
                     HVIALDTLGATLDQPLVMIHNGTGIYETRNEDDIILSYNSAEIRMVQSDVADEDLGYA
                     LAITKEGTLVIGTPSANDGRGKVYFSKTPLDSLSGDIDLSTEDSLDEFEVVLGAEEND
                     HLGTYAATGDINGDDVDEIFVVAPDAGEDDQGEIYIYDSHFDLIGTVSGSDGYCATSV
                     FVNNYISAVPDDLFLGPDHKSLNANLQRHDAPQEANMSQHGFLLSGETDFEEEIDIDE
                     ANIDAIIGDDLYQAAALGDINHDDEDDLALASSDGTISLFFGPIEMGTDLSEEDADVM
                     ITDGEVLDGFGKMLTIGDVTGDGIEDIIVGAPDYGDNLTGAVFVIFGRMTWPSSVSIT
                     SDTTLLTVLGESANDQIGGDFLLGDLNNNGVNELYTTKGAHDVYRVSLLVTAPGGSYE
                     LRGTNGGCGYSLAQNINNNPKQQISPFVLILIFLYTLVIPRLCTKC"
BASE COUNT          739 a          803 c          862 g          843 t
ORIGIN      
        1 aacaattaaa gaagccgtaa ataatattgt tactgtcttt gcgcaagccg aatgttggcg
       61 atcctcgttc cttcgtccta tcgtcctttc gtctcccttt acctgtaccc cgacgtctgt
      121 cggcgggcgg gttcggcgaa aagagcgtca tcaaaagttt gtcgtcggct gttgagtttt
      181 tccaactcta tggcgatcct gaattgcttt tcgatgtgac ttttggccgc cgtgagcagg
      241 gtgtcttgcc cccgttgcag gtctttgagc gtttgtcgca ggggcacgtc gggtgagata
      301 cccacaccct caaccagcga accgtccgca agcacaacgg gccaattggt gtaacaaaag
      361 gggaccttgc tgggaagtct ggcacaggag tatgtcccgc ccgcaccagc ggttgtctga
      421 ccgatgatcc tgccacgccc tgtcatttta aaagcggccg aaaacatgtc acacgctgag
      481 cggcaatagg aattggtgag tagtaccacg gggccgccgt acatgtagag atcggggtac
      541 gtggtgtagt tcatggagag acgcagtttg gatttgttct gtacaatgca gtttcgcgat
      601 ttgggatggc aggtttgcct gagcaaggtt tgatctggca taaaaaaact ggccatgcgg
      661 cacacctcgt cgccatagcc gccgtttgta ttcctgagat cgatgacaat tcccttggtc
      721 tggcgctgag acaggcctcg taaatgattc ctgaactgtg aggacacaga aggttcctgg
      781 ccctttttgt gtttgaaacc ggtgaccttg atgtaaccta cgtttccggg aagcgttgtg
      841 gactggacaa gcgggcggct tgattcccat cgccaaaagg aacgctcggt cagttcaacc
      901 cctgcgggga cgctgagaaa gatgtctttt tcttttcgct gttggtcaaa aaaagtgaac
      961 gtctgtggcg tgtacgtttt tttacttgtg cccggaactg tggcgaggag atctgtgctg
     1021 tacgcccgga tccattgagg tgatcccatg aggtgctttt ggaaatcgtt gagcacctgc
     1081 gctacgggct ggtgttggac cctggtcagt tcaaaacctt ttctgatgcc ggatcggtag
     1141 gcgggtgagt tgacgctgac ctcagagaca atgtatttgt tttcgatctc attgatcaca
     1201 aaaccgggat taaaacgctc tggcagggtg gggttgatga tcaaacagtg actgtcgcgc
     1261 aattcctgca gaatgttggt caacaattcg tagtgttttt gatcggtcgt gtgactccgt
     1321 accaagggga tgtacttttc cctgagggcg tcccagtcca ctcttttgag ccatgtgtag
     1381 gcgtagtttt gttcgagtgt gttgagaagg aatttgacat cgtgcaggcg ggccttgtct
     1441 gtgagggcct gtgcgggtga cacgcacaac agacagaaaa aaataaaaga cagagaaaat
     1501 gaaacgaagc ttgggtgtct cacagttacg gacgtgccta gcacatcatt ttatgtctgt
     1561 cgagagagac aagctcaaag cctcaacatt tggtgcacag acgaggaatg accaatgtgt
     1621 acaggaaaat caaaattaaa acaaagggcg aaatctgttg tttgggattg ttgttaatgt
     1681 tctgtgccag tgagtaccca cagcccccgt tcgtccctct taattcatac gatccaccgg
     1741 gcgcggtgac caagagtgaa acgcggtata catcatgggc acccttggtt gtgtagagtt
     1801 cgttcactcc gttattgttc aagtccccca gcagaaagtc tcctccaatc tggtcattcg
     1861 cactttctcc gaggacggtg agcagggtgg tgtccgaagt tatcgaaacg gaacttggcc
     1921 aagtcattct gccaaagata acaaagacgg cacccgtcag gttgtctccg taatcgggtg
     1981 cccccacaat gatgtcttca atcccgtcac cggtaacatc accgatcgtt aacatcttcc
     2041 caaaaccatc gagcacctcg ccatctgtga tcatgacatc tgcatcctcc tcagagaggt
     2101 ctgtgcccat ttcgatgggg ccaaaaaaga gactgatggt gccatcagac gatgccaagg
     2161 ccagatcatc ttcatcgtcg tgattgatat cgcccagagc ggcggcttga taaaggtcat
     2221 cgccaatgat ggcatcaatg ttggcctcat caatatcaat ctcctcttcg aagtcagtct
     2281 cgcccgacaa gaggaagccg tgttgactca tgttggcctc ttgaggggca tcatgtcgtt
     2341 gcagattggc gttaagggat ttgtgatccg gtcccagaaa aaggtcatcg ggcacggccg
     2401 agatgtaatt gtttacaaat acagaggtgg cacagtaacc atcactgcca gacaccgttc
     2461 caatcaaatc aaaatgggaa tcataaatat aaatctcacc ttggtcatcc tctcccgcat
     2521 ccggtgccac aacaaaaatc tcatccacat cgtccccgtt gatgtcgcct gtggccgcat
     2581 aggtgcccag atgatcgttt tcctcagccc ccaaaacgac ttcaaattcg tccagactat
     2641 cctctgtgct caaatcaata tcgccggaca atgaatcaag gggtgttttt gagaaataga
     2701 cctttccccg cccatcgttg gcgctggggg ttccgatcac gagtgtgccc tctttggtga
     2761 tggccagggc atagcccaag tcttcatctg ccacgtcgct ttgtaccatc ctgatctcgg
     2821 cgctgttata gctgaggatg atgtcatctt catttcgggt ttcgtatatc cctgtcccat
     2881 tgtgaatcat gaccaagggc tgatccaggg ttgcccccaa cgtatcgagt gcgatcacat
     2941 ggacggtcac ctcactgctg gcactttcgt tgttgatatc tgtgacagtc agttttgcca
     3001 cgacatcacc ttggctgtaa tacgtcgtga gtgttggggc ttgcgcagta gaatccgaaa
     3061 aggtggcata gcctgattcg atggaccagc tgtagataag gtcctctcct gctgagtcgg
     3121 tgccatcaag ggcgactttg ctgttgatga caaccgtctg gtcgggtccc gcattggcga
     3181 cgggaggatt attggtaatg accggcctgc tgttcgcgtc gccggggaag gttcgggcat
     3241 tgatttc
//
LOCUS       DATDUN010000382         1486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_601585, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000382 DATDUN010000000
VERSION     DATDUN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601585"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VJC18_05355"
     CDS             complement(<1..311)
                     /locus_tag="VJC18_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HLD44843.1"
                     /translation="MFSRTNKPIKPRALKKGDTIRIVAPSSPFEKQPFWNGVTVLENL
                     GFRVTYNESIFSKIPYLAGTDERRAREFIDALEESTSQAVLFARGGYGAMRLLPWLDK
                     YL"
     gene            836..>1486
                     /locus_tag="VJC18_05360"
     CDS             836..>1486
                     /locus_tag="VJC18_05360"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HLD44844.1"
                     /translation="MNDYSAFLVLIVIVSAGLLLGRIKIKGISLDLSAVLFVALLFGH
                     WGLQVSAIFQTMGLCLFIYAVGVQAGPGFFDSFGKQTLKHILLAVLLVVVAALTAWVC
                     FLIYDIDMSMAVGLLTGALTSTPGLAAAIEASQNPALASIGYGIAYPFGVIGVILFVY
                     LVPIFFRVNVKKEEQDYQQEVIEQHPQIMHRHFMVNNKNIFGKTIAELNIRTLTGAT"
BASE COUNT          361 a          332 c          364 g          429 t
ORIGIN      
        1 agatatttat ccaaccaggg caaaagtctc atggcgccat acccaccacg cgcgaataaa
       61 actgcctgac tggtcgattc ttcgagggca tcgatgaact cccgtgcgcg tctttcatcg
      121 gtgccagcca gataggggat tttggaaaaa atggattcgt tgtacgtgac tcgaaaaccc
      181 agattttcaa gcactgtcac accattccaa aagggttgtt tttcaaaagg cgaactgggg
      241 gccacgatgc ggatggtgtc accttttttg agggccctgg gtttgatggg tttgtttgtt
      301 cgagaaaaca ttgggagatt acaaacacta aacggaatgt ttggcaagtt tttgattggg
      361 cgattcgact tccctcgaca gtactcggga taaatcgctt atcacaaatc tggtttcatt
      421 gattcattga ttccttcctt tccttgtata ttcagccgga ataactatgc ataaatagga
      481 tacatgtttt ggacggaaag aggctgtttt gtggagccat aaataaaata gccctgaaaa
      541 cgtgagctta aaaaacagac atttttggca tgtcgtatgc atcattgata gtcatgagcg
      601 ggctagtcaa caaaaagaat ttctaccaag tggtcaaaag tttcgggttc ctgaccgcta
      661 ttaagctttt actcaccaaa gaaagaacct tcctcggctt cctcatgacc cagcacctgt
      721 tttaacccct ctttaagaag gttcccaaac actttcaaat taccgattca aatttatttt
      781 gctgttccaa aaacccattt gtggtatccc gagggcggaa tttcgaggga tatttatgaa
      841 tgattattct gcttttcttg tattgattgt gattgtgtct gccggattgc tattggggag
      901 aatcaaaatc aaagggattt cactggacct ttccgcggtg ctctttgtgg cgctcttgtt
      961 tggtcactgg ggcctgcagg tgtccgccat ttttcaaacg atgggattgt gcctgtttat
     1021 ctacgcggtg ggggtgcagg cggggccggg tttttttgac tcttttggca aacaaacgct
     1081 caagcacatc ttgttggctg tgttgctggt ggttgtggcg gccctcacag cctgggtttg
     1141 ctttttgatc tacgatattg acatgtcgat ggctgtgggg ttgttgactg gtgcgctcac
     1201 cagcacgcct gggttggcgg ccgcgatcga agccagccaa aatcccgctc tggcctccat
     1261 tggttacggc atcgcgtacc cgtttggtgt catcggggtg atcctctttg tgtatctggt
     1321 gccgattttc ttcagggtca atgtcaaaaa agaagaacag gattaccagc aggaagtgat
     1381 cgaacagcac ccgcagatca tgcaccgaca cttcatggtc aacaataaaa acatctttgg
     1441 caagaccatc gccgaactca atatccgaac actgactggc gctacg
//
LOCUS       DATDUN010000383         1313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_601679, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000383 DATDUN010000000
VERSION     DATDUN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601679"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1313
                     /locus_tag="VJC18_05365"
     CDS             <1..>1313
                     /locus_tag="VJC18_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLD44845.1"
                     /translation="MQMDLKANTGPYGSHCLTVPMNACSKSLPLFLCDNVHFNVTTYY
                     SNIPPTGAYQGYGAPKGSFALQLASAELAKELGLDHLAFLEKNRVHDGSMLEILRCLG
                     EGREGVAQKVSSCGLGPALQKGSEMIKWGQKEVSDDPDVKIGKGVAIIQQGSGLPGLD
                     SANATVSLLGDGTFMLLSGGTDLGTGLDTLTTKMVAETLCVDMNSVSMIAADTDVTPY
                     DDGAYASSGTFFSGSAALNAAKALKEKILHTASQMLGEPMETLELVYPAGVKGKRKTL
                     SFVEISRFTQCGTGAGQLSATANFTTDKAAIPYGAHFCQVAVNVRTGKVKIQKYYALQ
                     DCGTPINPELALGQIYGGVLKTIGHSLFEQMILDEKGKCLNANLLDYKVPMINDLPED
                     FKAVLVDTDDPFGPFGGKSISEIACNGASPAIAAAIHDAVGVWMR"
BASE COUNT          294 a          310 c          389 g          320 t
ORIGIN      
        1 ccatgcaaat ggatctcaag gccaacacgg gtccttatgg atcgcattgt ttgaccgtgc
       61 ccatgaacgc gtgttccaag agtctgccct tgtttttgtg tgacaacgtt cattttaacg
      121 tcacaactta ttattccaac atcccgccaa caggcgcgta tcaaggttac ggggcaccca
      181 agggttcctt tgccctgcaa cttgcgtcgg cagaactggc caaagagttg gggctggacc
      241 atctggcttt tttggaaaaa aatcgtgtgc acgacggttc catgctcgaa atcctgcgtt
      301 gtctggggga agggcgcgag ggagtggccc aaaaagtttc cagttgcggc ttgggtcccg
      361 cgctccaaaa gggatcggag atgatcaagt ggggtcagaa agaggttagt gacgaccccg
      421 atgttaagat tggcaagggt gtggcgatca ttcaacaggg atcaggtctg ccgggactgg
      481 attccgccaa tgctacagtg agcctgcttg gtgacggaac gttcatgttg cttagcgggg
      541 ggaccgattt ggggactggg ttggatacac tgacgaccaa gatggtggcc gaaacattgt
      601 gtgtggacat gaactctgtt tcgatgatcg cggccgatac cgatgtgacg ccctacgatg
      661 acggtgccta cgcctccagt gggacgtttt tttcgggctc agcggcgctc aacgcggcca
      721 aggcgctcaa agagaaaata ttgcatacgg catcgcaaat gctaggtgaa cccatggaga
      781 cgctggagct ggtttatccg gctggcgtga agggcaaaag aaaaaccctg tcttttgtag
      841 aaatttcgcg ttttacgcag tgtgggaccg gggcagggca gttgtctgcc acggccaact
      901 ttaccactga caaggcggcc attccttatg gggcgcattt ttgtcaggtg gcggttaatg
      961 tgcgtaccgg aaaagtgaag attcaaaaat attatgcctt gcaggattgc gggaccccca
     1021 tcaaccctga actggcgttg ggtcagattt acggtggcgt gctcaagacc attggccatt
     1081 ccctgtttga acaaatgatt ctggatgaga aaggcaaatg cctcaatgcc aatcttttgg
     1141 attacaaggt gcccatgatt aacgaccttc ccgaggattt caaggctgtg cttgtggata
     1201 ctgatgaccc ctttgggcca tttgggggca aatcgatttc cgagattgcc tgcaatgggg
     1261 cctcgcctgc gatcgccgcg gccattcacg atgctgttgg tgtgtggatg cgc
//
LOCUS       DATDUN010000384         1683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_309423, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000384 DATDUN010000000
VERSION     DATDUN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309423"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VJC18_05370"
     CDS             <1..223
                     /locus_tag="VJC18_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44846.1"
                     /translation="EHEDQPLLKRTFAAQLHRSGLTNEAITVLDKLVETLMQKGDRQG
                     AMEVINQIILMNPPNVENYRQILNQMKNS"
     gene            438..1466
                     /locus_tag="VJC18_05375"
     CDS             438..1466
                     /locus_tag="VJC18_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HLD44847.1"
                     /translation="MPKTKNNAIMKWLFIFSFVVAFLVIFGGFVRLTRSGLSIVEWNP
                     ISGVMPPIGAQAWQEEFAKYQLTPEYIKINTGMTLPEYQFIFYMEWVHRLIARLAGLF
                     YAIPVFYFLFKKIIPFKEFGIYIVMGLLFIGQAFMGWYMVASGLVDSPAVSHIRLTIH
                     LLLALSLFGIVLWTALGHKYGFPDTSKKVKWSLPSRLSFWLTLVLLIQITYGGMTAGL
                     KAGHVSDTWPLMFGKIIPPNLFNAWSDLFESPQTIVFVHRWFGWLGMILVPVVYYIVK
                     KQNYPADILNGFKWLTGLVALQIILGIFTILSYVNIVIALIHQANALALFGLALYLIH
                     RFRALDRK"
BASE COUNT          432 a          435 c          368 g          448 t
ORIGIN      
        1 agaacatgaa gatcaacctc tgctaaaacg cacgttcgca gcacaactgc accggtcagg
       61 ccttacaaat gaagcgatca ccgtgcttga taaactcgtt gaaaccctca tgcaaaaagg
      121 ggacaggcag ggagccatgg aggtcatcaa tcaaatcata ttgatgaatc cgccgaatgt
      181 ggagaattac cgccagatac tcaaccaaat gaaaaattcc taggactaca gaatacccat
      241 cgcaattgat gggtattttc ttttctgcgc ttcaccctta atgcgaacct gctcctgttt
      301 cgaagtgctt attcattatg acatatagtc acccggcttg tgacaaaagt tttaacagct
      361 caaaaagcct gcgtgctata atccttgcga tttgcaggtc gagccggacg agacctcatc
      421 aacactggag aaatatcatg ccaaaaacca aaaacaacgc catcatgaaa tggttgttca
      481 tttttagctt cgtggtagcc ttcctcgtta tcttcggggg atttgtgcgg ctgacccgct
      541 ccgggctttc cattgtcgaa tggaatccca tcagcggcgt gatgcccccc atcggtgcgc
      601 aagcctggca ggaggaattc gccaaatatc aactcacgcc ggagtacatc aagatcaaca
      661 ccggcatgac gctcccggaa tatcaattca ttttctacat ggagtgggtc caccgcctga
      721 tcgcgcgcct cgcaggtttg ttctatgcga ttcctgtgtt ctatttcctt ttcaaaaaaa
      781 tcatcccatt taaggaattc ggcatttata ttgtgatggg actgctcttt atcgggcagg
      841 ctttcatggg ttggtacatg gtcgccagcg gattagtgga tagcccggcc gtcagccaca
      901 tccgccttac cattcacctg ttattagcac tctccctctt cggaatcgta ttgtggacgg
      961 cgctcggtca caaatacggc ttccctgaca cgtcgaaaaa agtaaaatgg tcactgccgt
     1021 caagactatc cttttggcta acgcttgtgc tgttgattca aatcacctac ggcggaatga
     1081 ccgctggact caaggctggt cacgtttcag atacatggcc tctcatgttt ggaaaaataa
     1141 tcccacccaa cttgtttaac gcatggagcg acttgtttga atccccgcag acaattgttt
     1201 tcgtccaccg ctggtttggg tggctgggaa tgatcctggt gcctgtggtg tattacatcg
     1261 tcaagaaaca aaactaccct gccgacatcc taaatggatt caaatggttg acaggtttgg
     1321 ttgctctgca aatcatccta gggatattca ccattctttc ttatgtcaac atcgtgatcg
     1381 cgctgatcca ccaagccaac gcgcttgccc tattcggact ggcgctttat cttattcaca
     1441 ggttcagggc attggatcgg aaatagacga gctaaacaac aaaaagcgat gatggaaatg
     1501 atccatcatc gccttttttt actcagctat ccttcactct cgcctcttca cggggaatgt
     1561 tctaccgatc tagccgcgcg caattccgct cacaaaaatg atcataatca ggatccagga
     1621 gggatattcg ttctctattg acccatctcg gccagagcgg tatcgatctt ttcctggaaa
     1681 aca
//
LOCUS       DATDUN010000385         1880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_22597, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000385 DATDUN010000000
VERSION     DATDUN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22597"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..713
                     /locus_tag="VJC18_05380"
     CDS             135..713
                     /locus_tag="VJC18_05380"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLD44848.1"
                     /translation="MYRYKVDETKFYLALKKAGFRSLSDFAQRNPVNRMTLFHYFKGQ
                     GPFSESYYTICASLGLDPLSLLSQVSAGIPCEFVDEILPIAELATKDNKQLAVMIIGS
                     RAKGTSKQYSDWDLGLTTGSAPLTTHDYFRIKRVLTEAADDLPRSLDVVNLDQAPHWF
                     LEKMDYTPIFLSGNRSAHDFFLGVLHGSQKKC"
     gene            691..1074
                     /locus_tag="VJC18_05385"
     CDS             691..1074
                     /locus_tag="VJC18_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HLD44849.1"
                     /translation="MVVKKNVKLLKSLNNLKSAVRFYEKDTKAPDEIRFIALSKAFEV
                     CVEYAWKELKTRVEEEGLDAESPKETIRQSARIGLIASAEIWLDFISVRNEGVHDYFD
                     IPESDYLELAKCLLKECNKHLLLKS"
     gene            complement(1079..>1880)
                     /locus_tag="VJC18_05390"
     CDS             complement(1079..>1880)
                     /locus_tag="VJC18_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLD44850.1"
                     /translation="KICALLKIRVYELQTVPFVHVPLGPDWRGHLPKLLALLRKRWQA
                     LRLTQDEVRNLLSHMSSATTTNDILAGNITDKNLMVFEELLLLFHIETNPWELWDEQI
                     VSANVKRIELGRLIRDRLSQGGMLSPIRLARLAGISNSEKGYIVHGYRDPSRREVRRL
                     LAVLGMSLEGDETPVQTYLQEAIDWHRRPYQFGPNQTPPSSSKQEWCQRMTGIFLETG
                     HSVSSVCHFFGCDRSAVENIFTGNFVWNDPLVIRLSEIFRMCFVNREG"
BASE COUNT          521 a          473 c          402 g          484 t
ORIGIN      
        1 tagtggggtt ttttagaaag cgttttgcca acctcacagc ggatcagcgg ttcgaagcca
       61 cccggaacca aatcaatacg ctttttgtat aaatatatat acattttgta ttatatttgt
      121 taatataaaa gggtatgtac cggtacaaag tcgatgaaac caaattttac ctggcactca
      181 aaaaggcggg atttcgttca ttatcggatt ttgcccaaag gaatcctgtc aatcgcatga
      241 ccttgtttca ttacttcaaa ggccaggggc ctttcagcga gtcatattac accatctgtg
      301 cctctttggg tcttgacccg ttatccctgc tgtcacaagt atccgcgggc attccctgcg
      361 agtttgttga tgaaattctg cccattgcag aactggccac caaagacaac aaacagctgg
      421 cggtgatgat cattggttca cgtgccaagg gcacatcaaa acaatattcg gattgggatt
      481 tgggtctcac cactggctcc gccccattga ccacacatga ctattttaga ataaaaagag
      541 tcctcacgga agccgctgac gatcttcccc gttcgcttga tgtcgtcaac ctggatcagg
      601 caccccactg gtttctcgaa aaaatggatt acacgcctat ttttctgtct ggcaatcgtt
      661 ctgctcacga tttttttctg ggggtattac atggtagtca aaaaaaatgt taaactactc
      721 aagtccctga acaatctgaa atcggcggtg cgtttctacg aaaaagacac aaaagcgcct
      781 gacgagatca gattcatcgc cctgtccaag gcctttgaag tttgtgtgga gtacgcgtgg
      841 aaggagctca aaacccgtgt tgaagaagaa ggtctggatg cagaatcacc caaggaaacc
      901 attcgtcaat cagccagaat cggattgatt gcctctgcag aaatctggct cgattttatc
      961 tcggtacgca atgaaggcgt gcacgattat tttgacatcc ctgaatccga ctatctggaa
     1021 ctggccaaat gtctgttgaa agaatgtaac aaacatctct tgctcaagtc atgatcgttc
     1081 acccttccct gttcacgaaa cacattctga agatctcgga caacctgatg acaagcggat
     1141 cattccaaac aaaattcccg gtgaaaatat tttcaaccgc cgaacgatca cacccaaaaa
     1201 aatggcacac actgctcaca ctgtgtcccg tttccaaaaa aatgcctgtc atgcgctgac
     1261 accattcctg ttttgaagaa gaagggggtg tctgatttgg tccaaattgg taaggtcgtc
     1321 ggtgccaatc aatggcctcc tgcagatagg tctgcacggg ggtctcatca ccctcaaggc
     1381 tcatgcccag cacagccagt aaacgcctga cttcacgccg ggaaggatca cggtaaccat
     1441 gaacaatata acctttttct gaattgctga tgcctgccaa tcgggccaac cggatgggtg
     1501 aaagcatccc accctgactg agtctgtcgc gtatcaatct gcccaactca atccgtttga
     1561 cattggcaga aacaatttgc tcgtcccaca gctcccaggg attcgtttca atatgaaata
     1621 ataaaagcaa ttcctcaaat accatgagat tcttgtctgt gatattgcca gcaagaatgt
     1681 cattcgtagt cgtcgcgctc gacatgtgcg acaacaggtt cctgacctca tcctgagtca
     1741 atcgcaacgc ctgccagcgt tttctgagaa gcgccaacaa ttttggcaga tgaccccgcc
     1801 aatccggccc aagaggaacg tggacaaaag gcacggtttg taattcataa acacgaatct
     1861 tcagcaacgc acatattttc
//
LOCUS       DATDUN010000386         1317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_103619, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000386 DATDUN010000000
VERSION     DATDUN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103619"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VJC18_05395"
     CDS             <1..709
                     /locus_tag="VJC18_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HLD44851.1"
                     /translation="HEFATIGGYPKLYDMRDDSERLNNYVQHSIIEPTLGRDILSLHA
                     VDKPALLRQLFWYVSRMPAQIVSYEKILGCLQGKGNSATLVHYAELLRLAFIIVPLSK
                     FSQKANRTKRSLPKWIIPNQALIDPSVKQEALRDFIFENLVGSHFLNLLFGRRDYELV
                     YWREDNDEVDFILTKNNEPVLTIEVKSARVRGVVSLDLLIRNGICCPLVVITQKNIEQ
                     FLLTTSIEEMLACFIES"
     gene            complement(835..>1317)
                     /locus_tag="VJC18_05400"
     CDS             complement(835..>1317)
                     /locus_tag="VJC18_05400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HLD44852.1"
                     /translation="HEQQKTAPYSARGGSVSGVDLLAAGSDYTFWTNKPCHLSQDIDG
                     TSFSAPYTTRVIRMMRQINPQLKGETVIAILKASAEDLGTQLPEDQGAGRLVASRCFF
                     LAWLMRPGIRGPKKADVLAKELCIEHELRELRWLARRIKSRQRHNVSVDQSYGVYEKI
                     "
BASE COUNT          331 a          313 c          324 g          349 t
ORIGIN      
        1 tcatgaattt gcgacgattg gcggctatcc aaaactttat gatatgcgag atgattctga
       61 acgccttaac aattatgtgc aacacagcat tatagaacca acactgggcc gtgatatttt
      121 gtcactgcat gctgttgata agccagcact cctgcggcaa ctcttctggt atgtttcgcg
      181 catgcctgca cagatcgtga gttacgaaaa aattctggga tgcttgcaag gcaaagggaa
      241 ttctgcaact ctggttcatt atgctgaatt gttgcgattg gccttcatca tcgttccgtt
      301 atctaaattt tctcaaaagg cgaaccggac gaaacgctct ttgccaaaat ggatcatccc
      361 caaccaagcg ctcatagatc cttctgtgaa acaagaggct ctgcgtgatt ttatttttga
      421 aaatctggtg ggctcacatt ttttgaatct tctttttggc cgcagagatt acgaacttgt
      481 gtattggcgc gaggacaacg acgaagttga tttcatcctt acaaaaaaca atgaacctgt
      541 tttgacaatc gaagtcaaaa gcgccagagt tcgcggagtc gtgtctttgg atttgctcat
      601 aaggaacggt atctgttgtc cactcgtagt gattacacaa aaaaacatcg aacagttttt
      661 actcacaacc agcattgaag aaatgttagc gtgctttata gaatcctgag cgtccgcttc
      721 tgccaaagga cagatgagtc ctctggctca aaaggtggat cagcctctgg ctgagacgaa
      781 gccatgtatc gtacggtacc gggcaacgca tttcggtgga ccacaccccc cgatctaaat
      841 tttttcatag acaccgtacg attgatctac ggagacgttg tgacgctggc ggcttttgat
      901 gcggcgagcg agccagcgta attcgcgaag ctcgtgttct atgcagagtt ctttagccag
      961 gacatctgct ttctttgggc cccgaattcc cggtcgcatg agccaggcca gaaaaaaaca
     1021 acgcgaggcc accaagcgcc cggccccctg atcttcggga agctgtgtgc ccaggtcttc
     1081 cgcactggcc ttaaggatcg caatgactgt ctctccttta agttgcgggt tgatctgacg
     1141 catcatgcgg atgacacggg tggtgtaggg tgctgaaaaa ctcgtgccat cgatatcttg
     1201 ggacaaatga catggtttgt tggtccagaa tgtgtaatca gatcccgcgg caagcagatc
     1261 cacaccgctc acagaaccgc cacgggcgga atagggagcg gttttttgct gttcgtg
//
LOCUS       DATDUN010000387         1700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_144595, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000387 DATDUN010000000
VERSION     DATDUN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144595"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VJC18_05405"
     CDS             complement(<1..299)
                     /locus_tag="VJC18_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44853.1"
                     /translation="MNKFLLLCCLLFSGVSQATEAPVICGLVTDLNNNPVESASVRWQ
                     AADIKTLTDQSGRFCLPKTDALITNSNNMTAGKEGYYITGQKIEQTTTQTTITLKP"
     gene            complement(330..662)
                     /locus_tag="VJC18_05410"
     CDS             complement(330..662)
                     /locus_tag="VJC18_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLD44854.1"
                     /translation="MTKNTLKEATPQNVANYVSYQEGSVVSREIISRKVGTVTVFAFD
                     AGQGLSEHTAPFDALITVVDGQAEIIIAGKPHTVQTNEMILMPANIPHAVKAHQKFKM
                     VLTMIRGE"
     gene            791..1354
                     /gene="cyaB"
                     /locus_tag="VJC18_05415"
     CDS             791..1354
                     /gene="cyaB"
                     /locus_tag="VJC18_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HLD44855.1"
                     /translation="MPNIEIKAAYPDHDRAKKILMSLNAKHVGTDHQIDTYFFSPQGR
                     LKLRESSLSGAQLIPYMRPDQQGPKKSDYLVIPIVDPEACKRLFTQILGQDVVVDKIR
                     DIYLIDNVRVHLDQVKGLGLFFEFEAVYQNAHDEPTEYHKVRELIGIFGIREQDLING
                     SYRELVKGTSKNHVRYEKLQSESKQGR"
     gene            1485..>1700
                     /locus_tag="VJC18_05420"
     CDS             1485..>1700
                     /locus_tag="VJC18_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HLD44856.1"
                     /translation="MNVFNDLLPDFICDVIASQGYRPTGQIFQLNSYENRVYEVALET
                     DRPIVVKFYRPGRWSTETLLDEHRVLQV"
BASE COUNT          424 a          379 c          446 g          451 t
ORIGIN      
        1 ggtttgagcg tgatggttgt ttgggtcgtg gtctgttcga tcttctgccc tgttatgtaa
       61 tagccctctt tgccggcagt catgttattg ctgttggtga tgagagcgtc tgtttttggc
      121 agacaaaatc tgccgctttg atctgtcagg gttttgatgt cggcggcttg ccagcgaaca
      181 gaggcgcttt cgacaggatt gttgttgagg tcggtgacca gaccgcaaat cactggggct
      241 tccgtggcct gcgagactcc agaaaacagc agacagcaca acaaaagaaa tttgttcatc
      301 cccccccctt ttttagaggt cctctttact cattcccccc tgatcatcgt cagcaccatc
      361 ttgaattttt ggtgggcctt cacggcgtgc gggatgttgg caggcatgag gatcatctcg
      421 tttgtttgca ctgtgtgtgg tttgccagca atgatgattt cggcttggcc gtcgaccacg
      481 gtgatgagcg catcaaacgg tgccgtgtgt tcgctcaggc cctgacctgc gtcgaacgcg
      541 aacacggtga ccgtgcccac ttttcttgaa atgatctcgc gactgacgac agagccctcc
      601 tgataagaga cgtaattggc aacattttga ggcgtggcct ccttgagggt gtttttggtc
      661 atgctgttaa ttgtcacagt tcttggtgat aatcaatcat gcgaaaatag gttgtaaaaa
      721 aacaggatag tcaatagcgg gcagggtgtg aaaacaagtg gtgactattt aaaaaaaaag
      781 ggggcgagcc atgcccaaca ttgaaatcaa agccgcttac ccggatcatg atcgggccaa
      841 aaaaattctg atgagcctca atgcaaagca cgtggggact gatcatcaaa tcgacaccta
      901 ctttttttct cctcaagggc gcttgaaact gcgcgaatca tcactcagtg gtgcccagtt
      961 gattccctac atgcgcccgg atcaacaagg tcccaaaaaa agtgattatc tggtgatccc
     1021 tatcgttgac cctgaggcgt gcaagagatt gttcacgcaa attttggggc aagatgttgt
     1081 ggtggacaag atacgcgata tttatttgat cgataacgtg cgcgttcatt tggatcaggt
     1141 caagggactg ggtttgtttt ttgaatttga ggccgtgtat caaaatgcgc atgatgaacc
     1201 gaccgaatac cacaaggttc gtgaactgat cgggatcttt gggattcggg aacaggacct
     1261 catcaatggg tcttacagag aattggtaaa gggcacctct aaaaaccatg ttcgttacga
     1321 aaaattacaa tctgagtcga aacaaggacg ataagcaaaa agtccgcaga cgtataaggc
     1381 acgctacgtt gaggactttt tgtgaaatcg gacgcagttg cagaccagag tgtaattttg
     1441 cagtagaaga tggtttttag aggtgcccta aagggcaata aatcatgaac gtgttcaacg
     1501 acctgcttcc cgattttatc tgcgatgtga ttgcctcaca gggctaccgt cccaccgggc
     1561 agatttttca actcaacagc tatgagaatc gggtgtacga ggtcgcactc gaaactgacc
     1621 gccccattgt ggtcaaattt taccggcctg gacgatggtc aaccgaaaca cttttggacg
     1681 aacaccgcgt tttgcaggtg
//
LOCUS       DATDUN010000388         1206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_185777, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000388 DATDUN010000000
VERSION     DATDUN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185777"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VJC18_05425"
     CDS             complement(<1..256)
                     /locus_tag="VJC18_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44857.1"
                     /translation="MLQAVLLQKKSRSPLIENLENDFAFTRVNNVAALKKQLFKKEFD
                     FVFIAEKDVLQLMQSVLTVYELDKELPIIAHDSPEIYNKIP"
     gene            complement(266..979)
                     /gene="mnmD"
                     /locus_tag="VJC18_05430"
     CDS             complement(266..979)
                     /gene="mnmD"
                     /locus_tag="VJC18_05430"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HLD44858.1"
                     /translation="MTQNFSWFKTDDGSYTLHRRDIDEIYHSRSGALTECRHVYLNPF
                     LQSSPQSHSPTWRVLDVGFGLGYNWLCYVNETYSNGQNQSPPPDFLDITSIEHEPEIL
                     TLPVTNEHLKLGKHQEELLNLLAEYKHNKVIARPHLRSQLMIGDLKEKLNQLVANNAK
                     FDVILHDPFSPSKTPECWSHEVFSLLRQCCDAETMLLTYSVAGHVRRALTEAGFEVKK
                     IPGHGTKREQLIAFYRMRR"
     gene            complement(976..>1206)
                     /locus_tag="VJC18_05435"
     CDS             complement(976..>1206)
                     /locus_tag="VJC18_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HLD44859.1"
                     /translation="VIGDNTQACLESGLFFGYLGLLESMIARIEAETRCPLKIIMTGG
                     LSHLFVSEMIRPVQHEPHLTSNGLRLIWEKCQ"
BASE COUNT          295 a          256 c          321 g          334 t
ORIGIN      
        1 aggggatttt gttgtagatc tcgggcgaat cgtgcgcgat gatcggcaat tccttgtcaa
       61 gttcgtatac cgtgaggacc gattgcatta gctgcagcac atctttttcg gcgatgaaga
      121 cgaaatcgaa ttcttttttg aacaactgtt ttttgagagc cgcgacgttg ttcacgcgtg
      181 tgaaagcaaa gtcgttttcg agattttcga tgagggggga gcgggatttt ttttgcaaaa
      241 ggacggcttg taacatgctt ttcgtttacc gcctcatccg ataaaacgca atcaactgtt
      301 ctcgcttggt gccgtgtccg gggatttttt tgacctcaaa accggcctca gtcagagccc
      361 ttctaacgtg ccctgctacg gaataggtga gtaacatggt ctccgcgtca caacactgtc
      421 tcaatagcga gaaaacttcg tgagaccaac attctggggt ctttgagggc gaaaatgggt
      481 cgtgtaaaat aacatcgaat tttgcattgt tggccacgag ttggttaagt ttttctttaa
      541 ggtcaccgat catcaactga gagcgtaaat gggggcgtgc gatgaccttg ttatgtttgt
      601 attcggcgag caggttgagc aattcctcct ggtgtttacc gagtttcaag tgttcatttg
      661 ttactggcaa ggtcagaatt tcgggttcat gttcgatgga ggtgatatcg agaaaatctg
      721 gtggagggga ctgattttgt ccatttgaat aggtttcgtt aacataacag agccagttgt
      781 aacccaggcc aaaccccacg tcgaggacgc gccaggtggg cgaatgcgat tgaggcgagg
      841 attgcaaaaa aggattcaaa taaacatgac ggcattcggt cagggccccc gaacgagaat
      901 gatagatttc atcgatgtct ctgcggtgca gggtataact cccatcatcg gttttgaacc
      961 aactgaaatt ctgtgtcatt gacatttttc ccagatcagt ctgagaccgt tgctggtcag
     1021 gtgaggttcg tgttgtacag gccgaatcat ttcggagaca aaaagatgag acagccctcc
     1081 cgtcatgatg attttcaggg ggcaacgggt ttcggcctcg atccttgcaa tcatcgattc
     1141 cagaagaccc agatatccaa agaacagacc tgattccaga caggcctgcg tgttgtcgcc
     1201 gatgac
//
LOCUS       DATDUN010000389         1866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_60805, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000389 DATDUN010000000
VERSION     DATDUN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60805"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VJC18_05440"
     CDS             <1..351
                     /locus_tag="VJC18_05440"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HLD44860.1"
                     /translation="AVMHFGYDIIDDLKEGMATYLANKGLQNVSHIIGRSLPFITSHE
                     ELCYEKKIKSVINDILCVRCGDCVIACQDGGHMAITADIERKPVVDNDKCVGCGLCRL
                     VCPVRGCVEINSCR"
     gene            complement(368..1075)
                     /locus_tag="VJC18_05445"
     CDS             complement(368..1075)
                     /locus_tag="VJC18_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44861.1"
                     /translation="MTLLKSPPTLKHLSRAYFELGTLGARAVGQKHLWPYRFSSAEEL
                     FCLTSEMARYDPRLFGILVEFVIFHWENLNPLCVRRFIKAMNAPQIAGVIFEFARGVL
                     KNFEFGCFADYITRGLEPIAPQLFFRSLTAPASHTMRLTAQTPVHEFLKWGFIARERP
                     VLHGATRQSLGSWNSKTRQNIILELAERHSSFTIHDYLGAIDHTISRQQALNDLKGSK
                     WVKMKGQKRGSRWVKKR"
     gene            complement(1072..1596)
                     /locus_tag="VJC18_05450"
     CDS             complement(1072..1596)
                     /locus_tag="VJC18_05450"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HLD44862.1"
                     /translation="MDKKQMLSAFERLDHKLPKSAKLLLGGGGAMVLAHGFPLATMDV
                     DALFYKTPFSEADVLSQIHEVAVELNIPHDWLNPYFGTFLYTLPPDYETRLKTIFRGH
                     LLEVNALGLDDLLILKCFAGRDKDIPHARALIKKGANIDFVAQHIHKMMDINIPKADQ
                     ARDFLDDLIDEVGG"
BASE COUNT          464 a          417 c          486 g          499 t
ORIGIN      
        1 gcagtcatgc actttggtta cgacattatc gatgatttga aggagggcat ggcaacctat
       61 cttgccaaca aagggctcca aaacgtttca cacatcatcg gccgttcact cccgttcatc
      121 acgtcacacg aagagttgtg ttacgaaaag aaaatcaaat ctgtcatcaa cgatatcctc
      181 tgtgtgcgtt gtggagactg tgtcattgcc tgccaggatg gtggtcacat ggcgatcaca
      241 gccgatatcg agcgcaaacc cgttgtcgat aatgataaat gcgtgggttg tggtctctgt
      301 cgcctcgtgt gtccggtacg ggggtgtgtg gagatcaatt cgtgtcgctg agtatggtat
      361 aacaatgtca cctttttttt acccaccggc tccctctttt ttgtcctttc attttgaccc
      421 attttgaccc tttaaggtcg ttcaaggcct gctggcgtga gatggtgtga tcaatggccc
      481 caagataatc gtggattgta aatgaggaat gacgttcggc cagttcaagg atgatgttct
      541 ggcgtgtttt tgagttccaa ctgcccaggg actggcgtgt ggcgccatgc agaacgggtc
      601 gctctctggc aataaacccc catttcaaaa attcatgtac tggtgtttgt gcggtcaagc
      661 gcatggtgtg gctggctggt gctgtgagag acctgaagaa cagctgagga gcaataggtt
      721 caagcccgcg ggtgatgtaa tcagcaaaac aaccaaattc aaaattcttc aagaccccac
      781 gagcaaattc aaagataacg cccgcgattt gaggggcgtt catcgccttg ataaatcgcc
      841 tgacacagag gggattgagg ttttcccaat ggaaaatcac aaactcaacc aagatgccaa
      901 atagccgtgg gtcataacgc gccatttcag aagtcagaca gaacagttct tcagcactgc
      961 tgaaacggta gggccagagg tgtttttgcc ccacagccct ggcacccaag gtgcccaatt
     1021 caaaataggc gcgtgacagg tgtttaaggg taggcgggga tttaaggagc gtcatccccc
     1081 gacctcgtcg atcaggtcgt ccaaaaaatc ccgggcttgg tctgcctttg ggatattgat
     1141 atccatcatt ttgtgaatgt gttgtgccac gaaatcaata ttcgcgcctt ttttgatcaa
     1201 ggccctggcg tgagggatgt ctttgtctct tccggcaaaa cacttgagga tgagcagatc
     1261 atcgaggccc agtgcattca cttccagcaa gtgaccacga aatattgttt tgagacgggt
     1321 ttcataatcg ggaggcagag tgtacagaaa cgtgccaaaa taggggttga gccagtcgtg
     1381 tgggatgttg agttccacgg ccacttcgtg aatttgggac agtacgtctg cttcagaaaa
     1441 tggggttttg taaaagagcg cgtccacatc catggtggcc aggggaaaac cgtgggcgag
     1501 caccatggca ccgccgccgc ccagcaaaag ctttgctgat ttgggcaatt tgtgatccag
     1561 ccgttcaaag gcggagagca tttgtttttt gtccatgttt cattatagat atacttatga
     1621 caagttgtca aatatattgt catattggag acacagctta tgcttgcaga gttggtgttt
     1681 ttggcagtac taatggaaaa gttctttgtc ccgatcaaat catcgaaaac gaagctgtct
     1741 gttggttgag aagggtttac cctgcccaat tggtgtaggt ctttccccat aatgcttttt
     1801 ccacactctg tctttcgatg taaccaaaaa gaagtgagtt gtagtctatg gcatcccaaa
     1861 acaggg
//
LOCUS       DATDUN010000390         1258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_435709, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000390 DATDUN010000000
VERSION     DATDUN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435709"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..260)
                     /locus_tag="VJC18_05455"
     CDS             complement(72..260)
                     /locus_tag="VJC18_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HLD44863.1"
                     /translation="MRTTLNLNEEIYQQAARATGIKLKTELIHLGLKALIRESARERL
                     STLHGAVKKAMAAKRRRM"
     gene            complement(473..>1258)
                     /locus_tag="VJC18_05460"
     CDS             complement(473..>1258)
                     /locus_tag="VJC18_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLD44864.1"
                     /translation="ANALRSLMTCLPFPGTYKNSLIKNEDAKRLMSVPFLPVGLWTAA
                     GGIYGNQAQVRAAKMAIRKQVAGFGRVNFFSDLTLSLLLHLSAAPLIKITRFGKRANE
                     SLKAFQPIHQLMQGVPSPEAMDNIGWRHAEPDKMGLLWCAPTISSEGNKAREAIQLAE
                     KIFYEHGFEMPATLTLVRPERLVCVLSIHFDKSDPAERERAHTLYHTLNHRFAEMGIY
                     PYRTSILTMEKMQYEGGKQEVLAALKKIFDPDGLISPGRYGMG"
BASE COUNT          301 a          334 c          299 g          324 t
ORIGIN      
        1 atctgtcagg gcctgttcgt ctggtttgcc tctgaagaaa tcaatccata cgctagtgtc
       61 gaccaggacc ttcacattct cctccgtttg gccgccatcg cctttttgac agcaccgtgc
      121 aatgtggaca atctctcgcg ggcagattcc cggataagag ccttgaggcc caaatgaatg
      181 agctcggttt tcagtttgat ccctgtcgcc cttgccgcct gctgatagat ttcttcattc
      241 aaattaagtg ttgttctcat atgcatcatt atgcatcttt tatatgcatt cgtcaacaaa
      301 acaacacaag cagtgggtgt gcgatctaac cgtgggtaaa ttgtttttgg tagtccccct
      361 ccaaatcatt gtaccgggga agacagagaa tatgggcaca agctgaaaag ttccttgtcc
      421 ttaaaaggcc cccaatgata ttgtgaaccc gagatcgtaa atcgatttca gtttatccca
      481 tcccatagcg ccccggggaa atcagcccat cgggatcaaa gatttttttg agtgccgcca
      541 gcacttcctg tttccctccc tcatactgca ttttttccat ggtcaggata ctggtgcggt
      601 acggataaat ccccatttcg gcaaaacggt gattgagcgt gtgatacaat gtgtgcgcac
      661 gttcgcgctc cgcaggatcg gatttatcaa aatggatgct caacacgcag accagcctct
      721 ccggccgcac cagagtcaac gtggcaggca tttcaaaacc atgttcataa aaaattttct
      781 ccgccagctg tatggcctcc cgtgccttat tcccttcgga ggaaatcgtt ggcgcgcacc
      841 acagaagccc cattttatct ggttccgcgt gacgccaccc aatgttatcc atggcctcag
      901 gggagggaac cccctgcatc agttgatgaa tgggctggaa ggccttaaga ctttcgttgg
      961 cccgctttcc aaaacgcgtt atttttatga ggggggccgc cgacaggtgc aacaacaagg
     1021 acaaggtgag gtcggaaaaa aaattcaccc tgccaaaacc agcaacttgt tttctgatgg
     1081 ccatcttggc tgccctgacc tgtgcctggt taccgtaaat tccacctgcg gctgtccaga
     1141 gccccacggg aagaaaaggc acactcatca agcgcttggc atcttcattc ttgatcagag
     1201 aatttttata ggtcccggga aagggcaggc aggtcatgag tgaacgcaag gcgttggc
//
LOCUS       DATDUN010000391         1166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_602410, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000391 DATDUN010000000
VERSION     DATDUN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602410"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..>1166
                     /gene="rodA"
                     /locus_tag="VJC18_05465"
     CDS             147..>1166
                     /gene="rodA"
                     /locus_tag="VJC18_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HLD44865.1"
                     /translation="MKQPFNKSFYFPLFVFALILSVIGIVNLYSATISLETNQLSDYF
                     KAQLIYTLVGIVIMLMASFVSISRWYSLSPFIYGFALALLVLVLLMGSKTHGSLSWLD
                     LGFVRLQPSEFGKFGLVMMLARFLANLQKDEPLSFGEMCFMGLVFIIPTGLVIAQNDL
                     GSSLFYGFIFGTMIFLQGVKFRYIVIALIVIGVLGMVSYTFVLKDYQKARIASFMKPE
                     LDPKGSGYHLVQSKIAVGSGRLMGKGYLKGQSHRLKFLPERHTDFIFPVLAEEWGFVG
                     GGVTLLCYFLFLMMGLNVASESSSRFGFFVAAGVTATYFWHLVINLGGVLGLMPLTGV
                     PLPFLS"
BASE COUNT          209 a          249 c          333 g          375 t
ORIGIN      
        1 gctgtttctg tggtggtgga acacgggcgt gggggatcgt ttgcggcgga acccgtggcc
       61 atgaaggtga tcgatgcttt ttttgaggct caagcccggc acgattcaga ggccagtgat
      121 gaaaaaaagg aaagcgagaa ctgatcgtga aacaaccctt taataaaagt ttttattttc
      181 ccctgttcgt atttgccctc attctgtctg tgatcgggat cgtgaatttg tattcggcca
      241 cgatttctct cgagaccaat cagctcagtg attatttcaa ggcccagctc atctacacgc
      301 tggttggcat tgtcatcatg ctgatggcgt catttgtgag tatctcccgc tggtatagtt
      361 tgtccccctt tatttacggt tttgccttgg cgttgctggt tctggtgttg ttgatgggct
      421 cgaaaacgca tgggtctctc agttggctcg atctgggttt cgtgcgtctg caaccctccg
      481 agtttggcaa gtttggtctg gtgatgatgt tggcccgttt tctggccaat ctgcagaagg
      541 atgaaccttt gtcctttggg gagatgtgct ttatgggttt ggtgttcatc atccctaccg
      601 gtcttgtgat tgcccaaaac gatctgggga gcagtctttt ttatgggttc atttttggaa
      661 ccatgatttt tttgcagggg gtcaaatttc gatacatcgt cattgccctg atcgtcattg
      721 gtgtgttggg tatggtcagt tacacttttg tgctcaagga ttatcaaaag gcccgcattg
      781 ccagtttcat gaagcccgag ctcgatccca agggttccgg gtaccatctg gtgcaatcca
      841 aaatcgcggt tggttctggt cgactgatgg gcaaggggta tcttaaaggg caatctcatc
      901 gtctcaaatt tttgccagag aggcataccg attttatatt ccccgtgttg gcggaggagt
      961 gggggtttgt aggagggggg gtgacgctcc tctgttattt cctgtttctg atgatgggtc
     1021 tcaatgtggc gtctgaatca agttcgcgtt ttgggttttt tgtggccgcg ggcgtgaccg
     1081 cgacgtattt ctggcatctg gtcattaatc tgggcggcgt gttggggctc atgcccctga
     1141 ccggcgtgcc cctgcctttt ttaagt
//
LOCUS       DATDUN010000392         4366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_185969, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000392 DATDUN010000000
VERSION     DATDUN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185969"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VJC18_05470"
     CDS             complement(<1..564)
                     /locus_tag="VJC18_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HLD44866.1"
                     /translation="MNSNKYYAWFKYVRRMPLRTRLILAFVVLILSSASATIMIGNTV
                     FSRTVDHMVQNKGQQIETTAMYAQLKDTTTTLFTSLIASGMIFGFIMTYLFSLWLVKP
                     IAKLAEAMNRVAEGDLDLKVRIESADELGKLARAFNRMVAAIKERDYKLMEFNENRFT
                     AVEKQISIGRLAAGVAHEINNPLTAVLS"
     gene            840..1346
                     /locus_tag="VJC18_05475"
     CDS             840..1346
                     /locus_tag="VJC18_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HLD44867.1"
                     /translation="MSTQKAITYVSDIILGRTGSIIDRKYQRNMIREYAQNNNIEIVA
                     WFEDEIYSEDLIMRPGIQQLLGYEEKYDLLLVERVWALSRQLPQLEPFFKEMDRKSVS
                     LKCSTTMWDCVSQQCRRRFDSSLFKPDVSQIKKEFECVQEKETESVTIKKPSQLFFLN
                     LIKDPLKS"
     gene            1357..3405
                     /locus_tag="VJC18_05480"
     CDS             1357..3405
                     /locus_tag="VJC18_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HLD44868.1"
                     /translation="MGTTLSHAHRQIAWNVSHIEVMYVLFGLAMIVFGWGLWRHIRYW
                     RRGTATHGRFDHRWLRLKLLFKEILWQTKIRDSMVPAVFHSLIFYSFVILVITTTIIA
                     LDFDLGTSFFKGYVYVFFTVLAELAGLLILVGVGIAWYRRIRKPKTLPHARGDTIALI
                     LIALLVLTGFLVEGLRLAVHPDPWAWLSPVGQVFSFIFYGMNISTGRLMHHWLWWTHT
                     VLGMAWIASIPFTKLFHVLSVPANIYLSKTTPRGELFRFDIEALMSQEDFDAEKFKLG
                     IEKPSDFTWKERLDFDACISCGRCEEVCPATLAKMPFSPRELIANLKKCVHDADQKTV
                     GVTTSQAEEGTSEQPQSVVGTAFDHEYLWYCRTCTACMEVCPARVEHVDTVIDLRRNE
                     VVMQGRVPTEATLALKLLETHGNAFGPQTERSDWIDQLGVRVVAPGESCDVIYWIGCC
                     SVFDPAKRKIATDLAVILQKCGISFGVLGSDEMCCGDPARVLGQEHLFQTIAKKQVEA
                     LNARKFKVLLVSCPHCYNVLANEYPFFGGHYRVLHHSEFLHEMLWSGILKPKFGQRRK
                     AVYHDPCYLGRYQKVYEAPREVLKTIPGLAMVEMKNHKNKSLCCGGGGGHFWMDIKKG
                     ERINNLRVQQAVDVGADTIVTSCAYCMQMLEDSVKIMGKGDDLQVIDLATLTLESLER
                     "
     gene            3478..3549
                     /locus_tag="VJC18_05485"
     tRNA            3478..3549
                     /locus_tag="VJC18_05485"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3510..3512,aa:Cys,seq:gca)
     gene            3629..3895
                     /locus_tag="VJC18_05490"
     CDS             3629..3895
                     /locus_tag="VJC18_05490"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLD44869.1"
                     /translation="MGASDFGFERLDVYQRALSFAQIAWQITQKIRNTSNRSWCDQFE
                     RAAMSIPLNIAEGSGRRFEKEKKSFYNIAQGSVFECVAVMSLGY"
     gene            3928..4013
                     /locus_tag="VJC18_05495"
     tRNA            3928..4013
                     /locus_tag="VJC18_05495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3962..3964,aa:Leu,seq:taa)
BASE COUNT         1129 a          875 c         1128 g         1234 t
ORIGIN      
        1 agacaggaca gcggttaacg ggttgttgat ttcgtgcgca acccctgcgg ccaggcggcc
       61 aatggaaatc tgtttttcga cggcggtaaa acgattttca ttaaactcca tgagtttgta
      121 atcgcgttcc ttgatggcgg ccaccatccg gttaaaggca cgtgcgagtt tgccgagttc
      181 gtccgccgat tcgatccgga ccttcagatc gagatcgcct tctgccaccc ggttcatggc
      241 ttcggcgagt ttggcgattg gtttgaccag ccacaaggag aacaggtacg tcatgatgaa
      301 accaaagatc atgcccgaag caatgaggct ggtgaacagc gtggtggtgg tgtccttcag
      361 ttgcgcgtac atggcagtcg tttcgatctg ttgccctttg ttttgcacca tgtgatcgac
      421 agtgcggcta aataccgtgt tgccgatcat gatcgtggcg gaagccgatg aaagaattaa
      481 caccacaaag gccaatatga gacgtgttct gagcggcatc cgccgtacat acttaaacca
      541 ggcatagtat ttattactat tcataatggt gctccttgaa ctgcgtgtat aaaataaata
      601 tacatgtata aaaatatttt ggcacctcct tttttagact tgttttgagc aaaggttatt
      661 atctcaataa taccaagtca ttaccccatg gcaggtcgtt tctgatgctg tcattgtgta
      721 gtcataagat atacagcttt attggctctg tgcttcgaaa taactgattg tcacgtaaat
      781 acaaggagtt aaataaatgg catgccgatt gctgtattca ttacaggagg catgaatata
      841 tgagtacaca aaaagccatt acatacgtga gtgacattat tttaggtcgg actggttcga
      901 tcatcgaccg aaaatatcag aggaacatga ttcgtgaata tgctcaaaac aacaatatcg
      961 aaattgttgc ctggtttgag gatgaaattt acagcgagga tttgatcatg cgaccgggga
     1021 ttcaacaatt gcttggttat gaggaaaaat acgatctgtt attggtcgaa agggtttggg
     1081 ccttgtcacg acagctgcct cagttggaac cattctttaa ggagatggac cgtaagagtg
     1141 tctccttgaa atgttccacc accatgtggg attgtgtgtc gcagcaatgt cgtcgtcgat
     1201 ttgattcgtc attattcaaa ccggatgtga gtcagatcaa aaaagaattt gaatgtgttc
     1261 aagaaaagga aacagaatcc gtaaccatca aaaaaccatc acaattgttt tttttgaatc
     1321 tgatcaagga tccattaaag tcataaaggg ggccatttgg gcacaacact ttcccacgcg
     1381 caccggcaga ttgcctggaa cgtctcacat atcgaagtca tgtacgtttt gtttggtctg
     1441 gccatgattg tttttggctg ggggctgtgg cgacacattc gctattggcg tcgcgggaca
     1501 gcaacacacg gacgttttga ccaccgttgg ttgcgcctga agttgttgtt caaagaaatt
     1561 ctttggcaga caaaaatcag ggattccatg gtgcccgctg tgtttcacag cctgattttt
     1621 tattcttttg tgattttggt gatcaccacc acgatcatcg ccttggattt cgatttgggg
     1681 acatcgtttt ttaagggata tgtgtacgtg ttttttactg tgttggccga gctcgcaggg
     1741 ttgctcatcc tcgtgggggt gggcattgcc tggtacagaa ggattcgaaa accaaaaacc
     1801 ctgcctcacg caaggggaga tacgatcgcc ctgattttga tagccctgct ggtcctcacg
     1861 ggttttttgg tggaaggatt acggttggct gttcatcctg atccttgggc gtggttgtct
     1921 cctgtgggcc aggtgttcag tttcatcttt tacggaatga atatctcgac ggggcggcta
     1981 atgcatcact ggttatggtg gacccacacg gtcttgggaa tggcttggat cgcatcgatc
     2041 cccttcacaa aactgtttca tgtgttgtct gttccagcaa atatttactt gtccaaaaca
     2101 actccccgtg gtgaattgtt tcgatttgat atcgaggcgc tgatgtctca ggaagatttc
     2161 gatgctgaga aattcaaact ggggatcgaa aaaccatccg actttacctg gaaggagaga
     2221 ctggattttg atgcctgtat ttcctgtggc cgttgcgaag aagtttgtcc tgcgacgctg
     2281 gccaaaatgc ctttttctcc gcgtgaactg attgccaatt taaaaaagtg tgttcatgat
     2341 gccgatcaaa aaactgtggg tgtgacgacc tcacaggcag aagaggggac ttcggaacaa
     2401 ccgcaatcgg ttgttgggac tgcgtttgat cacgaatatt tgtggtattg ccgtacgtgc
     2461 actgcctgca tggaagtgtg tccggctcgt gtggagcacg tggataccgt gatcgatctg
     2521 cgacgcaacg aggttgtgat gcaggggcga gtgcccaccg aagcgacttt ggcgttaaaa
     2581 ttgttggaaa cgcatggaaa cgcgtttgga ccacagacag agaggagcga ttggatcgac
     2641 caactgggtg tgagggtggt ggccccgggt gagagttgcg atgtgattta ctggatcggg
     2701 tgttgttctg tttttgatcc cgccaagaga aaaatcgcga ctgatcttgc cgtcatttta
     2761 caaaaatgtg gtatcagttt tggcgtgctt gggtccgatg agatgtgttg tggtgatccg
     2821 gcgcgggtgt tggggcagga gcatctgttt cagaccattg ccaaaaaaca agttgaggcc
     2881 ctgaacgccc gcaagttcaa agtattgttg gtttcgtgtc cgcattgcta taacgtattg
     2941 gcaaacgaat acccgttttt tggagggcat tatagggttt tgcatcacag cgaattttta
     3001 cacgaaatgc tgtggagtgg aattctcaaa ccaaaatttg gtcaaaggcg aaaagcggtt
     3061 tatcacgatc cctgttactt gggccggtat caaaaagtgt atgaggcgcc ccgagaagtc
     3121 ctcaagacaa tcccgggcct tgcgatggtt gaaatgaaga atcataaaaa caaaagcctg
     3181 tgttgtggcg ggggcggcgg ccatttctgg atggatatca agaagggtga gcgcatcaat
     3241 aatctgaggg tccaacaggc tgttgatgtt ggtgctgaca cgatcgtgac cagttgcgcg
     3301 tattgcatgc aaatgcttga agactcggtc aaaataatgg gaaaaggtga tgacttgcag
     3361 gtgatcgatt tggcaactct cacactggag agcctcgagc gctaagcaat atcaataaaa
     3421 tatcaatttg acacccatcg tctggacatt tataggacca gccctaaatt ctattttggc
     3481 acggtcgcca agtggtaagg ccaggctctg caaaagcctt atacgtcggt tcaattccga
     3541 cccgtgcctc aaaaatggtt acccaccaaa tggtgggtaa ccattttagt agttagtagt
     3601 tagtggttta ggaattccag gaaaaataat gggagcatca gattttggtt ttgagagact
     3661 ggatgtttat caacgggcgc tgtcttttgc tcaaattgct tggcaaatca ctcaaaaaat
     3721 aagaaataca tcaaatagaa gttggtgtga tcagtttgaa cgagcggcca tgagtattcc
     3781 tctgaatatt gctgagggct caggtaggcg gtttgaaaaa gaaaagaaaa gtttttataa
     3841 cattgcacag ggctctgttt ttgagtgtgt cgccgtgatg agtttgggct actaataact
     3901 aaaatccacc caaagggtgg attttctgcc cgggtggcgg aattggtaga cgcaagggac
     3961 ttaaaatccc tcggcctttt ttggctgtgc cggttcaagt ccggccccgg gcacaaaatt
     4021 tttgcatggc aaaaaattta gttgttggta gttcgaaatt ggtagtttgg aagagtccct
     4081 caaatattaa aaaatagtct ctcagagatt ctcgtgattt attttctgtc tgaacaacac
     4141 cggacaatac atgaaatggt acacttttgg caaaaacagc ttaggtcttt gtttgttaga
     4201 ccaaaaattt tttcattgac ggtatctggc gtcttattga tataaacagc ggacaagtca
     4261 tgagtcatgt gcgttttggt ctgggcattt ccaaacaaaa atgtggcgac tctattaccc
     4321 tcaaggagag actaactatg aaatccaaat tgatcatgtt gacctt
//
LOCUS       DATDUN010000393         1888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_309847, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000393 DATDUN010000000
VERSION     DATDUN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309847"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /gene="glmM"
                     /locus_tag="VJC18_05500"
     CDS             complement(<1..658)
                     /gene="glmM"
                     /locus_tag="VJC18_05500"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836902.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HLD44870.1"
                     /translation="MRKLFGTDGVRGEANKYPMVPEVALALGQAIARYFRNKKTTQRI
                     VIGKDTRRSSYMLEQAIAAGVCSMGRDAVLTGPLSTPGVAFLTRAMRADAGIMISASH
                     NAYHDNGIKFFDHEGYKLSDEIERAIEDLIEQGFNDDQRPTGDQIGKAFRIDDATGRY
                     VEFVKRTFPKDVTLDGINVVLDCANGAAYKQGPMALWELGAHVTSWGIKPNGVNINSE
                     C"
     gene            complement(765..1586)
                     /gene="folP"
                     /locus_tag="VJC18_05505"
     CDS             complement(765..1586)
                     /gene="folP"
                     /locus_tag="VJC18_05505"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HLD44871.1"
                     /translation="MKSLLTQHRPVIMGILNLTPDSFSDGQVNAGIDDFLAKAAQLIS
                     EGCAILDVGGESTRPDAKPVSLDEEKRRVLPFLEAFRKQYPDFPVSLDTKKYELAKQA
                     MPYGIQWLNDVSCFSDERFAELVRESDVCYVLMHSRGDAQTMTKLTQYPQGVVEGVAL
                     ELGLKLERLRHCGVAMSQVVLDLGFGFAKTPEQCLELIESLSGWEKRFDHNLLFGISR
                     KRFLQGLVGEVPPLDRDDISARLAVRAYAAGFDIIRTHNVALTKKYIADYLSLHS"
     gene            complement(1593..>1888)
                     /locus_tag="VJC18_05510"
     CDS             complement(1593..>1888)
                     /locus_tag="VJC18_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HLD44872.1"
                     /translation="IGLHHKADYSEATALAIDNEIKNIIQTNYDRTKKILLENRDKLD
                     AIAKALIEFEILDAQQINLVLAGETLPPICKPVAGANQAEPAITAPDPTPQAT"
BASE COUNT          446 a          531 c          436 g          475 t
ORIGIN      
        1 cacattccga attgatgttg acaccattgg gtttaatgcc ccaactcgtt acgtgcgcgc
       61 cgagttccca caaagccatg ggcccctgtt tgtaggccgc cccgttggca caatccaaaa
      121 ccacgttgat gccatcgagc gtcacatctt tgggaaacgt gcgtttgacg aattcaacat
      181 aacgccccgt cgcgtcatcg atcctgaagg ccttaccaat ctggtctccc gtgggtctct
      241 ggtcgtcatt gaaaccctgc tcaatcaagt cctcaatcgc acgttcgatt tcatcgctaa
      301 gtttgtagcc ttcgtgatca aaaaacttga ttccattatc gtggtacgca ttgtgtgacg
      361 cactgatcat gataccagca tcggcacgca tggcgcgggt caaaaaagcc acacccggag
      421 ttgacaaggg acccgtgagc acagcatcgc gccccatcga acacacacct gcggcaatgg
      481 cctgttcgag catgtaggaa ctgcgccgcg tgtccttgcc aataacaatg cgttgcgtgg
      541 tttttttatt gcgaaaatac cgcgctatgg cctgcccaag ggccaacgcg acttcgggca
      601 ccatggggta cttgttggcc tcacccctca cgccatctgt tccaaataat tttctcattg
      661 tcacccctga cttgtcatcc cagcgaaggc tgggatccaa atttttgata gctctggatt
      721 cctgcttacg caggaatgac aaaaaaactt aattgacagt ctctctacga gtggagactc
      781 aaataatcag caatatattt ctttgtcagt gccacattgt gcgttctgat gatatcaaaa
      841 cccgccgcgt aagccctgac tgccagccgg gcgctgatgt catcacggtc caaagggggg
      901 acctccccca ccaagccctg caaaaatctt ttgcgtgaaa taccaaatag caaattgtga
      961 tcaaaccgtt tttcccaacc tgagagactt tcaatcaatt ccagacactg ctcgggtgtt
     1021 ttggcaaaac caaatcccag atcgagaaca acctggctca tcgccacgcc gcaatggcgc
     1081 aggcgttcca gttttagccc cagttcaagg gcgacccctt ccaccactcc ctggggatac
     1141 tgggtcagct tggtcatggt ctgcgcatca ccacgagagt gcatgagcac ataacaaaca
     1201 tcagactccc tcaccaactc ggcaaatcgt tcatcagaga aacaactgac atcattcaac
     1261 cactggatcc cgtacggcat ggcctgtttg gccaattcat attttttggt gtcgaggctc
     1321 acaggaaaat cgggatactg ttttcgaaac gcctccagaa acggcaacac acgtcttttt
     1381 tcttcgtcca gactgactgg ctttgcatcg gggcgcgttg attcgccacc aacgtccaaa
     1441 atcgcgcaac cctcactgat caactgtgcg gctttggcca gaaaatcatc aattcccgca
     1501 ttcacttgtc cgtcagaaaa cgaatcgggc gtgaggttga gaatgcccat gatgacaggt
     1561 cggtgttgcg tcaatagcga tttcacaatc cctcacgtcg cttgcggggt tggatcaggc
     1621 gctgtgatcg caggctccgc ctgattcgcc cctgctactg gtttgcagat cggcggtaga
     1681 gtttctccag caagcaccag attgatttgt tgcgcgtcca gtatttcaaa ctcgatgagg
     1741 gccttcgcaa ttgcatcgag cttgtcccga ttttccaaaa gaattttttt ggtgcggtcg
     1801 taattggtct gaatgatatt cttgatctcg ttgtcgatgg ccagtgccgt ggcctctgaa
     1861 taatccgctt tgtggtgcaa gccaattt
//
LOCUS       DATDUN010000394         1162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_144851, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000394 DATDUN010000000
VERSION     DATDUN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144851"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VJC18_05515"
     CDS             <1..315
                     /locus_tag="VJC18_05515"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HLD44873.1"
                     /translation="IIRNRLKVEAAVSNARAFLKVRDEFGSFDAYSWRFVDGNRIINK
                     WKHLENLPSTSKESDAFSKDLKARGFKFVGSTIVYAHMQAVGMINDHLASCYRYKEIC
                     GA"
     gene            complement(344..>1162)
                     /locus_tag="VJC18_05520"
     CDS             complement(344..>1162)
                     /locus_tag="VJC18_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HLD44874.1"
                     /translation="KETLIIVTADESHGSELGDWGSAWVPAIVFAPEQNKLPKINASV
                     YGLIDTTLSVLDYFAKTPPDTPGRSFFRKYRKPREMVSFTADRLRWLDETGTRIECNS
                     QGKCQLCAAQSLIGPADCQFSESVDSPHWVQKAKWLDQNLNRLSDLQTLHFATGKKQV
                     VKKPWQDQWADNLVGAQYLDFAANTETEVFMKWKIIKAPADGAQMVLSLKESDHDIDE
                     PVPVLPVLHAGQTSELSFKIQNKTARANFSFHLLAETPLEMQLLDFTVVIKKKS"
BASE COUNT          286 a          286 c          287 g          303 t
ORIGIN      
        1 atcattcgca accgcctcaa ggttgaggcc gctgtttcca atgcccgcgc ctttctcaag
       61 gtgcgggatg aatttggttc gtttgatgcg tacagctggc gctttgttga cggcaacagg
      121 atcatcaaca aatggaaaca ccttgaaaac ctgccatcaa catcaaaaga gtctgatgcc
      181 ttcagcaagg acctgaaggc acgcggtttc aagtttgtgg gatcgactat cgtctacgcc
      241 cacatgcagg cagtcgggat gatcaacgat catctggcga gttgttaccg atacaaagaa
      301 atctgtggtg cctgagtgaa cgatgttgcg tgagaaatga gggttaggat ttctttttga
      361 tcaccaccgt aaaatccagc aactgcatct ccaagggggt ttcggcgagc aaatgaaaag
      421 aaaaattggc cctggccgtt ttattttgaa tcttgaacga aagctctgat gtttgtcctg
      481 catgcaaaac aggcaggaca ggcactggtt catcaatgtc gtgatcggat tctttcaggg
      541 agagcaccat ctgggcacca tcagccggag ccttgatgat tttccatttc ataaacactt
      601 cggtttccgt attcgccgca aaatccaaat actgggcccc caccagattg tcggcccatt
      661 gatcctgcca gggttttttc acaacttgtt ttttgcccgt ggcaaaatgc agggtttgca
      721 ggtctgacaa gcgattcagg ttctgatcaa gccattttgc cttttgcacc caatgcgggg
      781 agtctacgct ttcactgaat tggcaatccg caggccctat gagggactgc gccgcacaga
      841 gttgacactt gccttgggaa ttacactcaa tccgggttcc tgtttcatcc aaccagcgca
      901 aacgatcggc tgtaaaagac accatttcgc gcggcttgcg gtattttcta aaaaaactcc
      961 gccctggtgt gtctggcggg gttttcgcaa aataatccag aacagataat gtggtgtcga
     1021 tcagcccgta aacactggcg ttgattttgg gaagtttgtt ttgttctggg gcaaaaacaa
     1081 tggcaggaac ccaggcgctt ccccaatcac cgagttcaga cccatgggac tcatccgcag
     1141 tcacaatgat cagtgtctct tt
//
LOCUS       DATDUN010000395         2685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_144930, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000395 DATDUN010000000
VERSION     DATDUN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144930"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /gene="dacB"
                     /locus_tag="VJC18_05525"
     CDS             <1..752
                     /gene="dacB"
                     /locus_tag="VJC18_05525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HLD44875.1"
                     /translation="SMTISRQFAEGTEYVTASGGVSDEKIKYANASDPVQYAGTTFAW
                     VLKQMGIDFTGKITSGNARGPVVLLKDKSKELSLILRDLNKFSNNFTAEMVLKTVAAN
                     RQGAPGSTEKGVVLLKDFVSALGIDSSEYAIFNGSGLSQRNHLSPNVLNRVLIAAYQN
                     NKIRSDFMSSLSIAGVDGTLGNRLKADVLKGNVKGKTGTLSDVSALAGYLETRAKNMV
                     AFTILVNGPAAGAGGYFAMQEKLLLDIYESY"
     gene            916..1617
                     /locus_tag="VJC18_05530"
     CDS             916..1617
                     /locus_tag="VJC18_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HLD44876.1"
                     /translation="MKPWNAPITLLFWLALFITSQLPLLTLIVVLLLTQPDMLKAGSA
                     ALSSNALLLVLASLIHLASTVVFSVMFIRLKRGLSVRAYLALNKASLKTYLLWILFTL
                     LFASAAGLAEFLFNGHDSQIWFEKLWSSTTNHIPLLLSIFVLAPLSEEILYRGVLQTG
                     LAQSRLGFAGAVIIPSLLWAGTHLQYNAFDMGLIFLLGLLLGIARQRTQSLYVTIAMH
                     CAMNLFSVCLFLITR"
     gene            complement(1785..2399)
                     /locus_tag="VJC18_05535"
     CDS             complement(1785..2399)
                     /locus_tag="VJC18_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HLD44877.1"
                     /translation="MTDKRIILDLETKRTFEEVGGRNALDKLGISVVGVYQYETDTYS
                     TFLEKDFGALQNLLIDASLIVGFNHVYFDMPVLQPYLSVDVKKLPCFDIMLDLQKIIG
                     HRIGLDAVAQATLGVGKIATGLDAIRFYQEGRWDELKKYCLKDVEVTKEVFEYGIKNG
                     QVMFRSRFGPAIKEVKVKWTPYNKRQATKTMSENPAEPAQYKLF"
BASE COUNT          643 a          678 c          645 g          719 t
ORIGIN      
        1 acagcatgac catttcgcgg caattcgccg agggcacgga atatgtcaca gcgtctggtg
       61 gtgtgtctga cgaaaagatc aagtacgcca acgcctccga ccccgtgcaa tacgcgggca
      121 ccacctttgc ctgggtgcta aagcagatgg ggattgattt tacgggaaaa atcacctcgg
      181 ggaatgcccg gggtccagtg gtgttgctca aagacaaatc caaggaactc tccctgatct
      241 tgagggacct caacaagttc agcaacaact tcaccgccga aatggtgctc aaaacggtgg
      301 cggccaacag acagggcgcc ccaggctcca ccgaaaaagg cgttgtgctg ttgaaggatt
      361 ttgtttcggc attaggcatt gactcgagcg aatacgccat tttcaatggt tccgggctgt
      421 cgcaacgcaa tcatctctcg ccaaatgtcc tcaatcgcgt gctgatcgcg gcgtatcaga
      481 acaacaagat ccgttctgat ttcatgtcgt cgctatccat cgcgggtgtt gatggcacgc
      541 tgggcaatcg tctcaaggcc gatgtcctta agggtaacgt caagggcaaa acgggaacac
      601 tcagcgatgt ttcggctctc gctgggtatc ttgaaacaag ggccaaaaac atggtcgcct
      661 tcacaatcct ggttaatggc cctgcggctg gcgcgggtgg ctattttgcc atgcaggaaa
      721 aacttttgtt ggatatttac gagagctatt aaaggcgtaa agccggatca attgctacaa
      781 ctactccaat gagcaacaga caattggcaa ttgatgtagt tgatcgttga aacaattgaa
      841 gcggttgttt tgccttatgc cttatgcctt tagccttatg cctacacatg aacacttgaa
      901 cacttgaaca catacatgaa accctggaac gcccccatca cactgctttt ctggctggcc
      961 ctgttcatta cctcccaact gcccctgctc accctcatcg ttgttctgtt attaacacaa
     1021 ccagacatgc tcaaggcagg ttctgctgcg ctgtcatcaa acgcgctctt gctggtcttg
     1081 gcttcactga ttcatctggc aagcactgtt gttttcagcg taatgttcat tcgtctcaaa
     1141 aggggtttgt ctgtcagagc ctatctggcc ttgaacaagg cgtcactcaa gacatatctc
     1201 ttgtggattc tctttaccct gctctttgcc tctgcggctg ggctggcgga atttctgttt
     1261 aatggccacg attcacaaat ctggtttgag aagctctggt catcaacaac aaatcacatc
     1321 ccgctactgc tcagcatttt tgttttggcc cccctcagcg aggaaatcct gtatcgtggg
     1381 gtgttgcaaa cggggctggc ccaatcgcgc ttggggtttg ctggcgccgt catcatccct
     1441 tccctgctgt gggcaggcac ccacctgcaa tacaatgcct tcgatatggg cctcatcttt
     1501 ttattgggtc tcctgttggg catcgcccgc caacgcacac agtcgctcta tgtcacgatc
     1561 gccatgcatt gcgcgatgaa cctgttttca gtctgtcttt ttttgatcac acggtaaggc
     1621 tgacgaaaac agaaattttt attctggccc tccaaacacc gaaacaggaa cattcgttcc
     1681 gtaatgatct tgccgaatgg tgtcattccc gcaaacaaga tcccgctttg tggtgatgac
     1741 tagtgcgagg ggctgttggc cgtaaactgt tggctgtggg ccatttaaaa caatttatac
     1801 tgtgcgggtt ctgcggggtt ttctgacatg gtcttggtcg cctgtctttt attgtacggg
     1861 gtccacttga ccttgacttc cttgatggcg ggtccaaaac gcgatcggaa catgacctgg
     1921 ccatttttga tgccgtactc aaagacctct ttggtcacct caacatcttt gaggcaatat
     1981 tttttaagtt cgtcccaacg accttcctga taaaaacgaa tcgcgtccaa gccagtggca
     2041 atttttccaa cccccagggt cgcttgcgcc acggcatcaa gaccaatacg gtgaccgatg
     2101 attttttgca gatcgagcat gatgtcgaag cagggtaatt ttttgacatc gaccgagagg
     2161 tagggttgca aaacaggcat gtcaaaataa acgtgattga aacccacaat caaagacgca
     2221 tcaatgagga gattctgcag ggcgccaaaa tctttttcca aaaacgtgga ataggtgtcg
     2281 gtctcgtatt gataaacccc cacaacggag atgcccagtt tatcgagcgc gttgcggcca
     2341 cccacttctt cgaaggttct tttggtttcg aggtccaaga tgatgcgttt gtcggtcata
     2401 aatttgcgtt gatcgacgat cgtggatcgt ggatcgtttg tgccctagag ggcatgaatc
     2461 gaaatcagct caaagcgcaa aggaaaacga atatcgaatc atgaacaaag aacaaggaat
     2521 ttcgaagtga tggtcattcc agcgatcctt cgactttgct taggatggtg agcttgtcga
     2581 accaaagctg aaatccatgt ttacattaat tctggatccc tgccttcgca gggatgacat
     2641 atttcgacat tcgacattcc ttgttcgata ctcaatattc atttc
//
LOCUS       DATDUN010000396         1487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_23135, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000396 DATDUN010000000
VERSION     DATDUN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23135"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VJC18_05540"
     CDS             complement(<1..847)
                     /locus_tag="VJC18_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44878.1"
                     /translation="MLQTILEFCLRLQKRFALLILIFGLVLAVICAPRAIKLLSTIKT
                     DLIHLLPDHYPSVHYTEKIQKTFNRRSSLFLILNSPDAQANLEAMFATKAYLETLPSV
                     QFVETQKRGYDFFDQNKLLLIDLKDLYQIHDRLKDKIQKEKLGGLYIDFSEDDQPEQA
                     VTFDDLIKKFKEEFNEGVRSRYKTNEDGTVIVLNIYPKSTDDSLSYFKKFGDEIDRHV
                     QQFDFKKYHPEMTFGYAGAIKTRVDQYDTLISDLKIAGIISLGSILLALYFYFWRFVK
                     GQRKDT"
     gene            complement(1087..>1487)
                     /locus_tag="VJC18_05545"
     CDS             complement(1087..>1487)
                     /locus_tag="VJC18_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44879.1"
                     /translation="GEGDPRQGLLVQNQEQCVAANTAGLSAIFFATNPDAAESTDLML
                     MAGAAVVSGGNPLAHLFDVARSTRTSFVAVPEGLTRPEAGAYLFITTRVPASEVVTID
                     PDNGTIYLGLLPIEEKPSFVLDEIQFLSGP"
BASE COUNT          381 a          360 c          353 g          393 t
ORIGIN      
        1 aggtgtcttt ccgttgacct ttcacaaaac gccaaaaata gaagtacaga gccagcagaa
       61 tcgaccccag actgatgatg ccagctattt tcaaatcaga gatgagggta tcgtactgat
      121 ctacccgcgt tttgatggcc cccgcgtacc caaaggtcat ttcgggatga tactttttga
      181 aatcgaactg ctggacatgg cgatcaatct cgtcaccaaa tttcttaaag taactcagac
      241 tatcatccgt gcttttggga tagatgttaa gcacgatgac cgtgccatcc tcgttggttt
      301 tgtaacggct gcgtaccccc tcgttaaact cctccttgaa ctttttgatg aggtcatcaa
      361 aggtcaccgc ctgctcgggc tgatcgtcct ccgaaaaatc aatgtacaac ccccccagct
      421 tttccttttg aatcttgtct ttgagccgat cgtgaatctg gtagaggtct tttaaatcaa
      481 tcagcaacaa tttgttctgg tcgaaaaaat catagccgcg tttttgcgtt tcgacaaatt
      541 gcacactggg aagggtctcg agataagcct tggtagcaaa catggcctcc agattggcct
      601 gtgcatccgg cgagttcaga atgagaaaga gactactgcg cctgttgaat gtcttctgga
      661 ttttttccgt gtaatgaaca ctggggtaat gatcgggcag aagatggatc agatcggttt
      721 tgatggtact gagcagtttg atcgctctgg gggcacagat aacggccagc accagcccaa
      781 aaatcaatat caataaagcg aatcgctttt ggagtcgtaa acagaattcc aatatcgtct
      841 gtaacatggt cgtacgatca actaccctaa cgcactgtaa tttcaaacga ttttttttaa
      901 aaaaaacacg caacttttgg atggtcgtga cgataatgct tatgtaagct cgaaagagct
      961 gactgacccc cttttaagac cttcgcattg ctaatcggca gtgagtgagg gtcttttttt
     1021 tttgcgaacc ctcctgaaat ccactcatct gcttcgtttc ctgcctcgtt tgcaaccgga
     1081 gccaactcat ggccccgaca gaaactgaat ttcatcaagc acgaaactgg gtttttcctc
     1141 aatgggaagg aggccaaggt aaattgtccc gttgtcagga tcaatagtga caacctctga
     1201 agctggtact cgggttgtga tgaagagata ggcacctgct tctggtcgtg tcaggccttc
     1261 cggcacagca acgaagcttg tgcgtgtgga acgtgccaca tcaaaaaggt gcgccagagg
     1321 gttgccacca gagaccacag ccgctccggc catgagcatc agatcagtgc tctcagcggc
     1381 atcggggttg gtggcaaaga aaattgcgga caggcctgct gtatttgccg ccacgcattg
     1441 ttcctgattt tgcacaagca ggccttgacg gggatcacct tcgccaa
//
LOCUS       DATDUN010000397         1447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_103918, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000397 DATDUN010000000
VERSION     DATDUN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103918"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VJC18_05550"
     CDS             complement(<1..532)
                     /locus_tag="VJC18_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HLD44880.1"
                     /translation="MKKAYIRTFGCQMNEHDSRQMLSLLAELGYEKTQEVTEADIIVL
                     NTCSIRAKAEHKIYSELGRLRPLKKTNPQLIICVAGCVAQQEKQKLSKRFPLIDIILG
                     PDQIGTLPSLVQQRLLSSSEQEHCVLCHTNLKEDGDYSFLNVLPSAGDVPVKSFVNIQ
                     KGCDNFCAYCIVPYVRG"
     gene            complement(587..931)
                     /locus_tag="VJC18_05555"
     CDS             complement(587..931)
                     /locus_tag="VJC18_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44881.1"
                     /translation="MQTRIVKLCKHKGCTNAATTMGYCRYHYLKNWKKIKDIQKKKAV
                     KNLNKYIDHIMHKNPDGYMDSIREDLRNQDQFVKKAEGYFSEDDFHDVMDELGSDDVD
                     RIIDSIKIDDSY"
     gene            complement(1090..1332)
                     /locus_tag="VJC18_05560"
     CDS             complement(1090..1332)
                     /locus_tag="VJC18_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44882.1"
                     /translation="MTTLLIIALIIIVVFIVLFYHRDRLYMKEDLKDVVSEDMRKRLN
                     MKTADAVAFENNLAKISRKKPLSQKIANEWDEITAP"
BASE COUNT          370 a          327 c          315 g          435 t
ORIGIN      
        1 gtccacgcac atacggcaca atgcaatacg cgcagaaatt gtcacagcct ttctggatgt
       61 tcacaaaaga tttgacgggg acatcaccgg cgctgggcag gacgttcaaa aaagaataat
      121 ccccgtcctc ttttaaattc gtatgacaca agacgcagtg ctcctgctcg gatgaactta
      181 atagtcgctg ttgcaccaat gagggcaagg tgccaatctg atccggtccc agaatgatgt
      241 cgataagggg aaaacgcttt gataatttct gcttttcctg ttgcgcgaca caaccagcca
      301 cgcagatgat cagttgggga ttggtttttt tgagaggtcg cagacggccc agttcagaat
      361 atattttgtg ttcggccttg gcgcgaatgg agcaggtatt gagcacgatg atatccgcct
      421 cagtcacctc ctgcgttttc tcgtaaccca gctctgccaa tagtgagagc atctgacggc
      481 tatcgtgctc attcatctga caaccaaatg tgcggatgta ggcttttttc actgaagctt
      541 atccaaaaac ctgtttgata tttgtttttt ggatagaaat agaatattaa tatgaatcat
      601 ctattttgat cgagtcgatg attctatcca catcatcact gcccaactcg tccatcacat
      661 cgtgaaagtc gtcttccgaa aaataaccct cggctttctt cacaaactga tcctgattgc
      721 gaaggtcctc acgaatggaa tccatgtacc catcgggatt cttgtgcatg atgtggtcaa
      781 tgtacttgtt gaggttctta acggccttct ttttttgaat gtccttgatt ttcttccaat
      841 tttttaaata atggtaacgg caatacccca tggtggttgc ggcattggta caacccttgt
      901 gcttgcataa tttgacgatt cttgtttgca tcctgcctat aattctctgt ttaatttcaa
      961 caagttgggc aagaagtagt cacccaaaaa aaagcctaat ccatggcaat cacttgcctt
     1021 tatcacaaag acccctagta gggaacatct cgaatgatgt caacaaataa ctgggattga
     1081 cgatatgcct taaggcgcgg tgatttcgtc ccactcattc gcgatttttt gggacagtgg
     1141 cttcttgcgg ctgattttgg cgaggttgtt ttcgaaggca accgcatccg cggttttcat
     1201 gttgagtctt ttgcgcatgt cctcagacac cacgtcctta agatcctctt tcatgtacag
     1261 acgatcgcga tggtagaaca gcacaatgaa aacaacgatg atgatgaggg ctatgattaa
     1321 taaggtcgtc atgtgatttt tccgtcgatc acatcataga gaactttttt gatctcgtcg
     1381 agtctttctc gtgatgtaat tttctgacca aaaatgtctt tgaggtacag ggtatccacc
     1441 acctgat
//
LOCUS       DATDUN010000398         1698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_23178, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000398 DATDUN010000000
VERSION     DATDUN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23178"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VJC18_05565"
     CDS             complement(<1..1311)
                     /locus_tag="VJC18_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44883.1"
                     /translation="MNKTPKEYHRVSTSSLLQFINENVTHKNVMIVSGARQVGKTTLI
                     KSILKDKPHLYINLEEKPSVADQIDRCVDFTDFESFLKERCQFDLETQILCIDEAQKS
                     VRLGSFVGFMKENWEGATVILTGSLINELHNETPRRPVGRETFLDLWPMTFREFVAAM
                     EQTSLVTVLDTFRPGNTITPMQHTRLLELFDQYLFVGGLPEIVTQFREGLDFQTRRRD
                     IFKTYEDDFVRYFSQENINLFRRAISAVADHVGSLSKDTHVVENNRPGYRKVAGIFSR
                     LEKWKLIIKCEQIGISPEHNARAPKRYLYDVGVLCEYRLRGLEQISVTQIDNPLLRIP
                     LGGIVENALALSLRNQFGDALYGLQLSPDAEVDFLVKHDRAAIPIECKMSLKFKTSYL
                     SGLATYLKKTQSKNPAFLFYGGMPLTEPTAGCFIVPYYFCDSVKR"
     gene            complement(1421..>1698)
                     /locus_tag="VJC18_05570"
     CDS             complement(1421..>1698)
                     /locus_tag="VJC18_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HLD44884.1"
                     /translation="LPVSEPVQFVSNLLGFDPLYLPCEGRFLAIVRPEHAKDIVKHLH
                     TLPHGAQARIIGEVTQQKNTSGVMLNTLTGGKRILDMPVGELLPRIC"
BASE COUNT          447 a          366 c          412 g          473 t
ORIGIN      
        1 cctcttgacc gaatcgcaaa aataatatgg aacgatgaaa caaccggcgg tgggttcagt
       61 gagtggcatg ccgccataaa acagaaaagc aggatttttt gattgtgttt tcttgaggta
      121 ggttgcgagt cctgacaaat agctggtttt gaatttgagc gacattttgc attcaatcgg
      181 gatagcggca cgatcgtgtt tgactagaaa atccacttcg gcgtcaggtg aaagttgcaa
      241 accatacagg gcatctccga actgatttct taaactcagt gccagcgcgt tttcgacaat
      301 gccccctagg gggattcgca acaacgggtt gtcgatctgg gtgacactga tctgttcaag
      361 tccgcgaagt ctgtattcgc acaaaacgcc cacatcatag agataacgtt tgggtgccct
      421 cgcgttgtgc tcggggctga tgccgatctg ttcgcatttg atgatgagtt tccatttttc
      481 tagtcgtgaa aaaattccag cgacttttct gtaaccaggt ctgttgtttt ctacaacgtg
      541 tgtgtctttg ctcagcgacc ccacatgatc ggctacggct gaaatagcac gtctgaacag
      601 attgatgttt tcttgcgaaa agtagcgtac gaaatcgtct tcgtaggttt tgaaaatgtc
      661 acggcggcgg gtttgaaaat cgagaccttc ccgaaattgt gttacgattt cgggaagccc
      721 acccacgaac agatattgat caaataattc gagcaagcgc gtatgttgca tgggggtgat
      781 tgtgttacca ggacgaaacg tgtccaacac ggtgacgaga gaggtttgtt ccatggccgc
      841 cacaaattct ctgaaagtca tgggccacag atccagaaaa gtttctcgtc ccacgggtcg
      901 gcgaggtgtt tcattatgca gttcattgat aagcgatcct gtgagaatga ccgtggcccc
      961 ctcccagttt tctttcataa aaccgacaaa actacccaat cggaccgatt tctgcgcctc
     1021 atcaatgcag agaatttgcg tttctaaatc aaattgacac ctttctttca agaacgattc
     1081 gaaatctgtg aagtcaacac accgatctat ttgatcggca actgaaggct tttcttccag
     1141 attgatgtat aagtgaggtt tgtcttttag aatggatttg attagtgtcg ttttgccaac
     1201 ctgtcgtgca ccagaaacaa tcatgacatt tttatgtgtt acattttcat taataaactg
     1261 caatagcgag gatgtgctaa cacgatgata ttcttttggt gttttattca tgatatcatc
     1321 atagcataga tattggcaaa ttcaagataa aatttacgca aaaacggcct aatttgcgaa
     1381 gttgttttcg cagaacaggc caaaacagcg agctggctta ttaacaaatc cttggcaaca
     1441 gttcgcctac gggcatgtcg aggatgcgtt tgccacctgt gagggtattg agcatgacac
     1501 cactggtgtt tttttgttgt gtgacctcgc caatgattct ggcctgagcg ccgtgaggga
     1561 gtgtgtgcaa gtgcttcaca atgtctttgg catgctccgg gcggacgata gccaaaaaac
     1621 ggccctcgca ggggagatac aaggggtcaa accccaaaag attcgaaaca aattgtaccg
     1681 gctcactgac ggggagat
//
LOCUS       DATDUN010000399         1804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_186116, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000399 DATDUN010000000
VERSION     DATDUN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186116"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VJC18_05575"
     CDS             complement(<1..617)
                     /locus_tag="VJC18_05575"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HLD44885.1"
                     /translation="MINRVDANDRFALARKRMVQEQLVARSITDERTLSAMNRVYRHC
                     YVEEALCEQAYTDAPLSIGEGQTISQPYIVALMTQALSLTGHEKVLEVGTGCGYQTTI
                     LALLADKVYTVERFKSLAIKARQRFKELALKNIVMRVGDGSVGWREAAPFDRILLTCA
                     APGFPQQFVEQLTPQGILIVPCKNQGGEQHLLRMTKTEVGCETQD"
     gene            complement(629..1369)
                     /gene="surE"
                     /locus_tag="VJC18_05580"
     CDS             complement(629..1369)
                     /gene="surE"
                     /locus_tag="VJC18_05580"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HLD44886.1"
                     /translation="MIDVLVTNDDGVEAKGIKALAKVLGGCRGVRVTVVAPRHEQSST
                     SHAISLRTPLRISKVKPNVYAVEGTPIDAVFAGVWIILKKKPDFIFSGINRGGNLGDD
                     VHYSGTVSAAMEGGLLGVPSVAISQLGKTEFDYTQSAAFARIMLEKVRKHTLPAGVVL
                     NVNVPEKANLCHYAVTKTGQRDYGGKYIEQRDPRGAPYYWIGGDQYKHISMKGSDCDA
                     VFSGKISITPLQVDITSYGHIETVRNWF"
     gene            complement(1446..>1804)
                     /locus_tag="VJC18_05585"
     CDS             complement(1446..>1804)
                     /locus_tag="VJC18_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44887.1"
                     /translation="SELQQQEQVSLAAQSSLAGAKEQDTSKKQTDVSPESGLGQSPDK
                     QSRTDRAVEKSALELLGSESFETRKQTPEPVPTVAPARRAKQFLRQYTFSELMLFGFG
                     ALVILVIIIVFAVLFL"
BASE COUNT          445 a          534 c          385 g          440 t
ORIGIN      
        1 aaatcttgtg tctcacaccc cacttctgtt ttggtcatcc gcaataaatg ctgttcacca
       61 ccctgatttt tacagggaac aataagtatt ccctgcgggg tcaactgctc cacaaactgc
      121 tgtgggaacc cgggagccgc acaggtcagc aatattctgt cgaacggcgc cgcttcacgc
      181 caacccaccg acccatctcc cacacgcatc acgatattct tgagcgccaa ctccttaaat
      241 cgttgcctgg ccttgatcgc caggcttttg aatctctcaa ctgtataaac cttgtcggcc
      301 aacaacgcca aaatcgtcgt ctggtaccca caacccgtcc cgacctccaa caccttttcg
      361 tgcccagtca acgacagtgc ctgtgtcatc agggccacaa tgtacggctg cgaaatggtc
      421 tgaccctcac cgatactcaa aggcgcatcc gtgtaggcct gttcgcacaa ggcctcctcc
      481 acataacagt gtctgtacac ccggttcatc gcggacaaag ttctctcatc ggtgatgcta
      541 cgcgccacca gttgctcctg aaccatccgt ttacgagcaa gtgcgaatct gtcgtttgca
      601 tcaaccctgt ttatcactaa aagctcctct aaaaccagtt tctcacagtc tcgatgtgac
      661 cataactggt gatatcaacc tgaagtgggg taatcgaaat ctttcccgaa aaaacagcat
      721 cacaatccga gcccttcatg gaaatatgtt tgtattgatc tccccctatc caataataag
      781 gcgctccgcg gggatccctt tgttcgatgt atttgccacc gtagtcgcgc tgaccagttt
      841 tggtcacggc ataatgacac agattggcct tttcgggcac attgacattg agcaccacac
      901 ctgcgggcaa tgtgtgtttt cggacctttt ccaacatgat tcgggcaaac gcagcgcttt
      961 gggtgtaatc aaactccgtc ttgcccagtt gtgaaatggc gactgaagga acacccaata
     1021 atcccccctc catggccgcc gaaacagtcc cggaatagtg aacatcgtca cccaaattac
     1081 cgccccggtt gatccccgaa aaaataaaat ctggtttttt tttgagaatg atccacacac
     1141 cagcaaaaac agcatcaatc ggtgtgccct ccacagcata tacattcggt ttcaccttac
     1201 tgatgcgcaa cggagttctg agcgatatcg cgtgagacgt cgaagactgt tcatgccgtg
     1261 gcgccaccac ggtcacacgc accccgcgac agccccccaa cacttttgcc aaagccttga
     1321 tgcctttggc ctcaacacca tcatcattgg taaccaacac atcaatcatg aggctgttaa
     1381 tactgattat tcagagaaag acaagccttt gtcactcatt ctctcggcat tttttggggg
     1441 tgctcttaca gaaacagcac agcaaacaca atgatgatca caagaatcac cagcgctcca
     1501 aacccaaaca acatgagttc cgaaaaagtg tactgacgca agaattgctt cgccctccgc
     1561 gcgggggcaa ccgttggaac aggctcaggg gtctgctttc gggtttcaaa agattcgctt
     1621 cccaaaagtt ccaaggctga tttctcaaca gccctatcgg ttctactttg tttgtctggc
     1681 gactggccaa gaccggactc cggagacaca tcagtctgct ttttgctggt gtcctgttct
     1741 ttggcgcccg ccagggacga ttgtgcggca agggacacct gctcctgctg ttgcagttct
     1801 gagg
//
LOCUS       DATDUN010000400         4231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_475902, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000400 DATDUN010000000
VERSION     DATDUN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475902"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..579
                     /locus_tag="VJC18_05590"
     CDS             187..579
                     /locus_tag="VJC18_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD44888.1"
                     /translation="MSQPKILIVDDEYFFIETIKMRLETLGYDVIFADNGADGMEEAL
                     AQKPNLILADMIMPDMGGLELITLLRTNETTRHIPIIALSALSEPQNIREAIEAGAND
                     YIVKPFESAELINKITSLLTERRAWGKT"
     gene            813..1160
                     /locus_tag="VJC18_05595"
     CDS             813..1160
                     /locus_tag="VJC18_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HLD44889.1"
                     /translation="MFQTLTPFYTVLLLIISNVFMTFAWYGHLKYKASPLYLAILVSW
                     LIASVEYCFQVPANRIGHGYFSTAQLKTLQEIITLTVFVFFSVFYMKEAIRWNHLVGF
                     GFMILAAFFIFKE"
     gene            1341..2012
                     /locus_tag="VJC18_05600"
     CDS             1341..2012
                     /locus_tag="VJC18_05600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HLD44890.1"
                     /translation="MKKFLVSICVVFILIFALGGYIFAKFKIMGHISRGTPISVYQNP
                     HEALLIIDLQNDLTDPAGKHPINLQQTDRVITNTNKLINIFNDAGRLIVYIKQIHETG
                     PIVSYFTKGALASGTKGAELDSRVLVRGNNILEKKVMDAFSNPELGKILAKHSVNRLY
                     ITGLAADQCIDRTTKAALNRKYDVVVIEDAVIASSDQVVSSKRQEFKSLGVSVLKTDE
                     LKLLQ"
     gene            complement(2065..2364)
                     /locus_tag="VJC18_05605"
     CDS             complement(2065..2364)
                     /locus_tag="VJC18_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLD44891.1"
                     /translation="MVFYVYILKCKNGRYYVGQTNNLSRRLRQHTVGDGCKYTTDFIL
                     DELIYYEIFSDRTQAVQREKQRKGWSRAKKEALILNQKPRLKLLGISHQSPRYHR"
     gene            3350..>4231
                     /locus_tag="VJC18_05610"
     CDS             3350..>4231
                     /locus_tag="VJC18_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLD44892.1"
                     /translation="MSYQSAYDESITNPEAFWGEAAKEIQWHKPYDRVFDGSRSPFFS
                     WFPGGELNTCYNCLDWHVEQGRSDQTAIIYDSPVANIIKKISYRELLGLVSRFAGVLT
                     ALEVRQGDTVILYMPMIPETVVAMLACARIGAVHSVVFGGFAPHELAIRIDDAKPRVV
                     ISASCGLEGKKIIEYKPLLDRALELATHKVQKCVLYQRAQAKAPLMAGRDIDWQIAMQ
                     TATPVACVPVKSTDPLYILYTSGTTGIPKGVVRDNGGHAVALKWTMKHIYGVQPGEVF
                     WAASDVGWVVGHSYIVYA"
BASE COUNT         1092 a          931 c         1019 g         1189 t
ORIGIN      
        1 actgctgttt ttaggacagc ctgttgattt tgttcaagat caaggcgtgt gcggcttctc
       61 ccggagggag gcgtactcaa atgtacgttg accgacggga aagacgcacc aacacagaga
      121 ttggacaaaa gcgacaggct actaaatgag gctaacagcc gaatttaggt tgaaagggaa
      181 ttttttatgt cacaacccaa aattctgatt gttgatgatg aatatttttt tatagaaacc
      241 atcaaaatgc ggctggaaac tttgggctat gacgtcatct ttgccgacaa tggtgccgat
      301 ggcatggaag aggccttggc acagaaacca aacctcattc ttgccgatat gatcatgccc
      361 gatatgggtg ggttggaatt gatcaccctc ctgcgtacta acgaaacaac cagacacatc
      421 cccatcatcg ccctgtctgc cctctcggag ccacagaaca tccgagaagc aatcgaggcc
      481 ggtgccaatg attatattgt caagcctttc gaatccgccg agctcatcaa caaaatcacg
      541 tccctgctca ctgaacgaag ggcttggggg aaaacttagg gggttcttag aggtggccat
      601 tattgtaaac gatcgattgc ttacgctgtg tttgaagttg atgggggcgc ctcaaggtaa
      661 ccatcttgag attccaaatt ggaatctcaa gatggagcga gtgatatgac aaaccttatc
      721 ccttttgaaa gaatagaaaa aagcatttta cgttattctg tttcgggagg ggaaatgttg
      781 ttttttaaca gcaaatgtgt tgtgttccct tcatgtttca aacactcaca cctttttaca
      841 cggttttgct gttgatcatt tcaaatgttt tcatgacctt tgcttggtac ggtcatctca
      901 aatacaaagc cagtcccttg tatctggcaa tcttggtgag ctggctcatt gcgtctgttg
      961 aatattgttt tcaggttcct gcgaacagaa ttgggcacgg gtatttttcc actgcacaac
     1021 ttaaaactct tcaagagatc atcacgctca cggtgttcgt atttttctcc gtgttttata
     1081 tgaaggaagc gatccgctgg aatcatttgg tgggatttgg tttcatgatc ttggcagcat
     1141 tttttatttt taaagagtga tgggggataa aaatgatggc agcactgcac ctatgtgcta
     1201 gcacataggt gcacatatca tggggctatg tctctgcatc ataccggatt agtttttcga
     1261 caaattgcat gcttgcatta tgttgaagga gttgagagag ggaaaatgat aggggaggac
     1321 gttgcacggg ggaaaaaatc atgaaaaaat ttcttgtttc aatttgtgtt gtatttattc
     1381 ttatcttcgc cttgggcggt tacatatttg caaaattcaa aatcatgggc catatttcac
     1441 gaggcactcc tatttctgtt tatcagaatc ctcatgaggc tctattgatc attgatttgc
     1501 aaaatgatct cacagatccc gcggggaaac accccatcaa tttacaacag acagataggg
     1561 ttattaccaa tacaaataaa ttgataaaca tctttaatga tgcgggacgc ctcattgttt
     1621 acataaaaca aatccatgag acagggccta ttgtcagcta ctttactaaa ggcgcgcttg
     1681 cgagtggcac aaaaggcgct gagcttgaca gccgtgtttt ggtgagagga aataatattt
     1741 tagaaaaaaa ggtgatggat gccttttcca acccggagct tggcaaaatt ttggcaaaac
     1801 actcggtcaa tcgtctttac ataacaggcc tggccgctga tcagtgtatt gacaggacaa
     1861 ccaaggccgc cttgaacagg aaatatgatg ttgttgtgat tgaggatgct gtcattgcgt
     1921 catcggatca agtggtttct agcaaacgac aagaatttaa gagtctcggc gtgtcagttt
     1981 tgaagactga tgagctcaag ttgctgcagt aaattctagc aggtagttga aaattgaatg
     2041 attgaggaat gcttggcaca tgtttcaccg atgatacctc ggcgattgat gagaaatacc
     2101 caaaagtttg agccgtggtt tttggttgag aatcaaagct tcctttttgg cgcgggacca
     2161 acctttgcgt tgtttttctc tttgaacagc ctgtgttcga tctgaaaata tttcgtagta
     2221 gataagctca tccaaaatga agtcagtcgt gtatttacag ccatcgccaa cggtgtgctg
     2281 tcttaatctt cttgagagat tgttggtttg ccctacatag tatctgccgt ttttgcattt
     2341 aaggatgtag acgtagaaaa ccatacgcct gatcttctgt tgtttgggaa tgagagtttc
     2401 ggatttggtg gaattttttt gtgcttcgac tccctcgatg aactcgggac aggtccctgc
     2461 ggtcgctcag cacaggttgc ttcgactact tcgacgaact caatacaagt cagtttatcc
     2521 cgagcgacta aaaggagtcg agggactcag cacgtcgcac ctggcctgag cccgtcgaag
     2581 accttcgact catgaaagcc tgttttggac aaaaaaaaga cccgcaacgc tcactcaagg
     2641 tgcctgccct gagcgagcga agcgagtcga agggctccgg ctgttggact tgaaccaacg
     2701 acctagcggt tactttctga cttgagtttc cccaagagtc ggactttatc ttcatccgtc
     2761 tgatattaac atcagcgagg atggcgggtg tagagtctct gcacttgccc ccctgaaccg
     2821 atcactggtt cagggtgcta gctcaggatt gccgacgact catgttgtgc gcatgatcgt
     2881 ttcgggttcc ctgatttagc ccgcttattc actctgctgt ttccagcaga ggctgctaac
     2941 ttggcatgca attcacgatg acaatttgca caaagcatca cacatttatc cagctcctct
     3001 ttaacgcttt tctgtcatcg tcaaccacaa gccttgtccg cccaaggcgg actcggcaca
     3061 gccgcttgct ctaccactga gctaagccgg aatgcgttct caataaaacg gaagagaacg
     3121 agcggcttta ctagactagt aaaaagtgcc ttgtcaaatg atattgcgga ggtagcgtaa
     3181 ccataacgga ttaccctggt tgttttcctt ccattgattt ttgaggaaat tcaagttact
     3241 ccgtcatcaa gcttatcaag attattttga ggaaattaaa gttaccccat cacaagacta
     3301 ccatgattat tgatcatgct tatttgttga gaatagaaaa ggactaaaca tgtcatatca
     3361 atctgcttat gatgaatcca ttaccaatcc tgaagccttc tggggcgaag cggccaaaga
     3421 aatccaatgg cacaaaccat acgaccgtgt ctttgatggg tctcggagtc cttttttcag
     3481 ctggtttccg ggcggagagc tcaacacctg ttacaattgt ttggattggc atgtggaaca
     3541 aggccgatcc gaccagacag ccatcatcta cgacagccct gtcgcaaaca tcattaaaaa
     3601 aatttcgtac cgtgaactac tgggattggt atcacgattt gcaggggttt tgaccgcact
     3661 tgaggtgcgc cagggagata ctgtcattct ttacatgccc atgattcccg agactgtggt
     3721 ggccatgctg gcctgtgccc gaattggggc cgtgcattct gtggtgtttg gagggtttgc
     3781 cccccatgag ctcgccatac ggatcgacga tgccaaacct cgggttgtga tttcggcctc
     3841 atgcgggtta gagggaaaaa agattattga gtacaaaccg ttgttggatc gtgctctgga
     3901 actcgctaca cacaaggtcc aaaaatgcgt cctgtatcaa cgggcccagg ccaaggctcc
     3961 cctgatggca ggtcgggata ttgactggca aatcgccatg caaacagcca cacctgttgc
     4021 ctgtgtgcca gtcaagtcca ctgacccttt gtacatcctt tacacctcgg gcaccaccgg
     4081 gattcccaag ggcgtggtac gtgacaacgg cggtcatgca gtagcactca agtggaccat
     4141 gaagcatatt tatggagtac agcctggtga ggtgttctgg gcggcttcgg atgttggttg
     4201 ggtagttggg cattcgtaca ttgtttatgc t
//
LOCUS       DATDUN010000401         1269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_269447, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000401 DATDUN010000000
VERSION     DATDUN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269447"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1269)
                     /locus_tag="VJC18_05615"
     CDS             complement(<1..>1269)
                     /locus_tag="VJC18_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HLD44893.1"
                     /translation="TILENNAIQSIATAAGYSNGALAASLAAYMTITNRLIPSWQAMI
                     WLILLGVLGVLFAFPFKKRFINDEQQPFPEGKACGIVMEGLHSGQDREGVFKTKLMMF
                     SMGLATVWKLCSSELVMKSLWSKLGYIPAYLDDFFYNTLNLKPSILDTQLKDLTVRLE
                     SDLVMYAFGGLIGIRIGSSLLLGALINYVILAPIMMKQGIIVGVGFKAITSWALWGGV
                     AMMTTASLLSFFAKPKMLISAFSGMFSNKKRNRDVLKDIELPMKVFVIGIPTVGLTTA
                     MVGHFFFHFSWWLGLIALPLVFIMTLISVHSTALTAITPSGPVGKITQLIYAVVSPGN
                     ITTNIMSACVAAETSSSASNLLMDIKPGYMLGAKPRQQALGHVLGVIVGVLVAVPVFY
                     MMINYDLTNLFSEKLPAPAGTVWKAVAELLT"
BASE COUNT          331 a          300 c          367 g          271 t
ORIGIN      
        1 gtgagcaact ctgccacagc tttccagacg gtgccggcag gcgcggggag tttttcggaa
       61 aaaagattag tgagatcgta gttgatcatc atgtaaaata cggggaccgc gacgagcact
      121 cccacaatca cacccaacac atggcccagg gcctgttgtc ggggtttggc tcctaacata
      181 taaccgggtt tgatgtccat gagcagattg gaggcactgc ttgatgtctc ggcggctacg
      241 caggccgaca tgatgtttgt ggtgatgttg cccggtgaca ccacggcgta gatcaattgt
      301 gtgattttgc caacgggtcc gctgggagtg atggctgtga gtgccgtgga atgaaccgaa
      361 atcagtgtca tgatgaaaac gaggggaagg gcaatgagac ccagccacca ggagaagtga
      421 aagaaaaaat gtcccaccat ggcagtcgtc agccctacgg tcgggatgcc aatcacgaag
      481 accttcatgg ggagttcaat gtccttgagg acatcacgat ttcttttctt gttggaaaac
      541 atgcccgaaa aagcggaaat cagcattttg ggtttggcga aaaaggaaag taacgaagcc
      601 gtggtcatca tggcaacgcc gccccacaag gcccaactgg tgatggcctt gaagccaacc
      661 cccacaatga ttccctgttt catcatgatg ggggccaaaa ttacgtagtt gatgagcgca
      721 cccagcagaa gagaggaacc gattctgatc ccgatgagtc cgccgaaggc atacatcacc
      781 agatcggatt caagacgtac ggtcaggtct ttgagttgtg tgtccaaaat cgatggcttg
      841 agattgagcg tgttgtagaa aaaatcgtcc agataagcgg gaatgtaacc cagctttgac
      901 cagagggatt tcatgacgag ctcggatgaa cacaacttcc agacagtcgc gaggcccatg
      961 gaaaacatca tgagttttgt cttgaacacg ccttctctgt cctgaccgga atgcaagccc
     1021 tccatgacga tgccgcaggc cttgccttcg ggaaagggtt gctgttcatc attgataaag
     1081 cgtttcttga aagggaaggc aaacaaaaca cccaagacgc ccaggagaat cagccagatc
     1141 atcgcctgcc aggaggggat gagacgattg gtgatggtca tgtaggccgc gagactcgcg
     1201 gccagcgcgc cgtttgaata gccggcggct gtggcgatgg actggatggc attgttctcg
     1261 aggatggtc
//
LOCUS       DATDUN010000402         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_310333, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000402 DATDUN010000000
VERSION     DATDUN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310333"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VJC18_05620"
     CDS             complement(<1..656)
                     /locus_tag="VJC18_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44894.1"
                     /translation="MTARPPTPPPFCAIPEPKDDAYTCYPRDPRLELSVYQIKKKQKA
                     SQIRRDIERHFRKYQDDESFFDALRTFKNQETQQIIDSLESQPIHNRQRILEALSDLA
                     DNILFAAYQRVFQRLRGKYGAPGFLSSYKQTGETPLAIVGMGKLGGREINYQSDLDLI
                     FVYAHKGETLGAPPVDNGQFFIKLAQQLMNTLSVMTATGRCYQVDTELRPSGNAGTLV
                     T"
     gene            complement(646..1983)
                     /locus_tag="VJC18_05625"
     CDS             complement(646..1983)
                     /locus_tag="VJC18_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44895.1"
                     /translation="MPSNLFQHSANPEEAENFFANFRKAFRQNHQKEFVPDETNLSFL
                     KIFGNSKFLCRFAINNTWVYEQYLGSKFKGTEKPLEQFTSEIKTIVTNEAQPDSTLKK
                     YKYQEFLRLTLKELSGMDQATTYRELSHLAAALVAHSVKKHHAELSHKHGISAGSTGE
                     YAIIALGKLGGQELNYSSDIDIIGLYDEDPTLSDLSRHQFYSQLFAQTGQHLSHVDKD
                     GFFFRVDWDLRPEGKTGVLANSLGAMERYYQTFGQEWERQAFIKAHVLLQTRNLGDRF
                     LVMLAPFVFRRSFDSKTIHNIWNMKTLIEQDRKNNNPEGINIKLDEGGIRDIEFFVQG
                     FQLLHGGKSTELRTTNTAEALLRLSQHQLITTQIKNVLTQNYFFLRRLESCLQMDNEQ
                     QTHVLKNDPRHKLAVARRLGFTHPEKEALSILEERLFNVMSATKRIFHEHYDR"
BASE COUNT          454 a          461 c          569 g          588 t
ORIGIN      
        1 gtgaccagag tgcctgcgtt tccggacggt ctgagttcgg tatccacctg atagcaacgg
       61 ccggtggcgg tcatcaccga caacgtgttc atgagttgtt gcgccagttt gatgaagaac
      121 tggccgttgt ccacaggggg ggcgcccaag gtctcgcctt tgtgtgcgta tacgaaaatg
      181 agatcgaggt cagattgata gttgatctca cggccgccca gtttgcccat gccgacaatg
      241 gccagcggcg tttcacctgt ctgtttgtaa gaactcaaaa aaccgggggc gccatatttg
      301 cccctcagtc tttgaaaaac gcgctgatac gccgcaaaga ggatgttgtc ggctagatcc
      361 gagagtgctt ccaagatccg ttgccggttg tggattggct gtgattcaag gctgtcgatg
      421 atttgttgtg tctcctggtt tttaaaggtt ctgagtgcgt caaaaaaact ctcgtcatcc
      481 tggtattttc gaaagtgtcg ttcgatgtcg cgtctgatct gcgaggcctt ctgttttttt
      541 ttgatttgat agacactcaa ctccaatcgc ggatccctcg ggtagcaggt gtaggcatca
      601 tccttgggtt caggaatggc gcaaaagggc ggtggcgttg gcggtctagc ggtcatagtg
      661 ttcgtgaaag atgcgtttgg tggcgctcat gacattgaat agtcgttcct ccaaaatgct
      721 caaggcctct ttttcgggat gcgtaaaccc caggcgtctg gccacggcca gtttgtgccg
      781 ggggtcgttt ttcaatacat gggtctgttg ttcgttgtcc atttgcagac aggattcgag
      841 acgccgcaga aaaaaataat tttgcgtcag cacgttcttg atctgtgtgg tgatcagttg
      901 gtgttgcgat agccggagca gggcttctgc tgtgttggtg gtcctgagtt cggtactttt
      961 gccaccgtgc agcagttgaa atccttgaac gaaaaattca atgtcgcgaa ttcccccttc
     1021 gtcgagtttg atgttgatgc cttcagggtt gttgttttta cggtcctgtt cgatcaacgt
     1081 tttcatgttc cagatgttgt ggatggtctt ggagtcgaaa gatcgtcgga acacaaaggg
     1141 cgcgagcatc accagaaatc ggtcacccag atttctggtt tgcaacagga cgtgggcctt
     1201 gatgaaggcc tgtcgttccc actcctgccc aaaggtctgg tagtaacgtt ccatagcacc
     1261 cagagagttg gccagcaccc ccgttttacc ctcggggcgc aggtcccagt ctacccgaaa
     1321 aaagaaaccg tctttatcga catgtgacag gtgctggccg gtttgagcga aaagttgtga
     1381 ataaaactga tggcgagaca gatcggacag ggtggggtct tcgtcgtaca gcccgatgat
     1441 gtcgatgtcg gagctgtaat tgagctcctg cccacccagt ttgcccagag cgatgatcgc
     1501 gtattcgccg gtactgccag cggagatgcc gtgtttgtgg gacagctcgg catgatgttt
     1561 tttgactgaa tgggcaacca gggcggctgc cagatgtgac agttcgcggt aggtggtggc
     1621 ctgatccatt cccgacagtt cttttagggt gagtcttaga aactcctgat acttgtattt
     1681 tttgagagtg ctatcgggtt gcgcctcatt ggtcacaatg gttttgatct cgcttgtgaa
     1741 ttgttccaaa ggtttctccg tgcctttgaa tttcgaacct aaatattgct cgtaaaccca
     1801 ggtattattg atggcgaaac ggcacagaaa tttggaattg ccgaatatct tcaaaaaaga
     1861 cagattggtt tcatcaggga caaattcttt ttgatggttt tgtctgaatg ccttgcggaa
     1921 attggcaaaa aaattctcgg cttcttccgg attagcgctg tgttgaaaga ggttgctcgg
     1981 cacgcccaca caattacatc aattgtttca atgatcaact acttcaattg acaattgaaa
     2041 gttgaaacag ttgaaataat tgaccaattg at
//
LOCUS       DATDUN010000403         4587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_393763, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000403 DATDUN010000000
VERSION     DATDUN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393763"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1394)
                     /locus_tag="VJC18_05630"
     CDS             complement(183..1394)
                     /locus_tag="VJC18_05630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLD44896.1"
                     /translation="MKQLAQRMNAIQPSPTLVIDARAKELKAQGKNVINFGVGEPDFE
                     TPAHIREAAKRAIDEGKTRYTPVAGTVSLKKTVMEKLRRENNLHYTTDEIIITNGGKH
                     ALFQIFMALVDEGDEVIVPSPYWVSYPDQIRVFGGRPVIIPTTDKTNFKITSAELEAN
                     ITNKTIAFVLNSPSNPTGAAYHPDELRALAAVCQNKGVYIISDEIYEHIVFDDFSVTS
                     IASLSPQSKQMTIIANGASKCYAMTGWRMGFAAGPAHIISAMSKAQGQSTSNVCSITQ
                     AACEEAYGGPQQAIKEMVCAFQERRDHIVAALNRLPGVTCKQPKGAFYVFPNISGLMG
                     RQTSRGPKLNTSQEFCMHLLEEYGVATVPGSAFGCENYFRISYATSLEEIKEGMNRIS
                     QLVQDLTKGHF"
     gene            1883..>4587
                     /locus_tag="VJC18_05635"
     CDS             1883..>4587
                     /locus_tag="VJC18_05635"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HLD44897.1"
                     /translation="MKIKRLFTKKGESPYQNLKFVKRTCEIKNPDGSVAFYADDVSVP
                     ESWSQVATEVLVQKYFRKAGVPLDQENGQLQLAENSRQKTGAEKDARQIFHRLAGCWR
                     FWGEKFKYFDTKEDAQAFYDETAFMLANQMAAPNSPQWFNTGLHFAYGINGPSQGHFY
                     VDPKTKELAKSTSAYERPQPHACFIQSISDDLVNDGGIMDLWIKEARLFKYGSGTGTN
                     FSSLRAKGESLSGGGRSSGLMSFLKIGDAAAGAIKSGGTTRRAAKMVIVDVDHPDVQE
                     FVNWKVVEEQKVACLVTGSQVIRKALSQIMKACQNKELKNDRFDPLKNKELGLAVRDA
                     RSQQVPQNYILRVIQFARQGFTSLDFAEYNVDWNSDAYATVSGQNSNNTVRVNNDFMN
                     AVRENADFSLKGRITDENIKTIKAKELWEQICSAAWNCADPGLQFDTAINEWHTCPQD
                     GRINASNPCSEYMFLDDTACNLASLNLVSFYDTQKGTFQIRDFAHATRLWTIILEISV
                     LMAQFPSQLIAQKSFDFRTLGLGYANLGALLMRMGLAYGSKEAVAITGAITALLTGEA
                     YATSAEMAKELGPFQKYYRNQEDMLRVIRNHRRVAYDAPSDEYEMLTILPQGIDQKNC
                     PPDLLQAAKESWDHALEGGEKYGFRNAMVSVIAPTGTIGLVMDCDTTGIEPDFALVKF
                     KKLAGGGYFKIINESVPPALKKLGYTDNQIQDIVNYCRGTGTLKSAPAINHKTLTEKG
                     FTADALEKLEIALTQAFDITFVFNKFTLGETFFKRDLKLDPKQYNEPSFNLLKQLGFT
                     DQDIERANNYCCGTMTVEKAPHIDPKHYPVFDCASKCGRYGTRFISADAHVEMMAAAQ
                     PFISGAISKTINMPNDATIEDISRIYFKSWGLMLKANAIYRDGSKL"
BASE COUNT         1184 a         1199 c         1060 g         1144 t
ORIGIN      
        1 catctcccga ctcactttcg cctgatgtcc caagagggtg tcggcatctt ttcctaacag
       61 ttcaatgagt ttttcgtgat tcatgttttg actccattca aagccttctg tctctggtcg
      121 taaaaacaac cagaaaccga cagcgagaaa tcctcgaaca cctctaaata ctatcatcgt
      181 acttagaaat gcccctttgt taaatcttgc accagttgac tgatacgatt catgccttct
      241 ttgatttctt ccaagctggt cgcataactg atgcggaagt aattttcaca cccaaaggcc
      301 gagcccggca cggtggccac accatattct tccagcaggt gcatgcaaaa ttcctgcgaa
      361 gtgttgagtt ttggtccccg gctggtttgt ctgcccatca aacccgaaat attgggaaaa
      421 acataaaacg cgcccttcgg ctgtttgcag gtcacacccg ggagtctgtt gagcgcagcg
      481 acaatgtgat cacgtctttc ctggaaagcg cacaccattt ccttgatggc ttgttgtggt
      541 cctccgtacg cctcctcaca ggccgcttgc gtaatggaac acacgttcga ggtcgactgc
      601 ccctgcgcct tgctcatggc actgatgatg tgtgccggcc ctgccgcgaa acccatgcgc
      661 caccccgtca tcgcataaca tttggaggcc ccgttggcaa tgatcgtcat ctgcttgctt
      721 tgcggggaca gggatgcgat gctggtgacg gaaaaatcat caaacacaat gtgttcataa
      781 atttcgtcag aaatgatgta cactccttta ttctgacaga ctgcggccag cgccctgagt
      841 tcatcgggat gataggccgc ccccgtgggg ttgctggggc tgttgagtac aaaggcaatc
      901 gtcttgtttg tgatgttcgc ttccagctcc gcgctggtga ttttaaaatt ggttttgtct
      961 gttgtgggga tgatgaccgg tcgtccgcca aaaacacgaa tctggtcggg ataactcacc
     1021 caatagggag agggaacaat gacttcgtct ccctcgtcca cgagggccat gaaaatttga
     1081 aacagcgcat gtttgccgcc attggtaatg atgatttcat cggtcgtgta gtgaagattg
     1141 ttttctcttc gcaatttttc catgacggtt ttttttaggg acacggtgcc cgccactggt
     1201 gtgtacctcg tcttgccctc atcaatggcc ctcttggcgg cttccctgat gtgcgctggc
     1261 gtttcgaaat caggttcacc cacgccaaaa ttgataacat tttttccctg tgccttcagc
     1321 tccttggctc gtgcgtcaat aaccagtgtg ggcgaaggtt gaatggcgtt cattcgttgc
     1381 gcaagttgtt tcatataaaa tccccaagaa tctgacgcct catatcaatg aacacacaat
     1441 aattgcaaac attcacaaca agatcattaa cccgctaacg atgtagattc atttgtgatt
     1501 ccatggcaat tagacatttt tgggtttttg ttcaaaaata acagagggat acgattcgta
     1561 aaagacgtca atacaaaaaa ggtgttgtct tataaccata agatattttt gttgtttttc
     1621 tgtcctgtca ctttgagcgc gacaacaatg acgtgttgcg tcaaagaaag aaaacaagat
     1681 ttcatgcggt tagatttttt ttattaaaac tgttgtcgac cacaatatat tgtggtttaa
     1741 tgcccaatgt tgcactatat gttgtggata agacatttat gtacatgtta tcatttggct
     1801 tcttttcttc ttcttatcct aaagcagctg ctgaattgtg gtttttaggt cgtaggacat
     1861 cgagctgggg ggcgttaaaa atatgaaaat caaaagacta tttacaaaaa aaggtgagtc
     1921 tccttatcaa aatctgaagt ttgtaaaacg aacctgtgag attaaaaatc ctgatggttc
     1981 tgtcgccttt tacgctgacg acgtgtctgt tcctgagtct tggtctcagg tcgcaaccga
     2041 agtattggtt caaaaatatt ttcgaaaggc gggggtcccc ctcgatcagg aaaacggtca
     2101 attacaactg gcggaaaact cccgacaaaa aaccggagcc gaaaaagacg cgcgccagat
     2161 tttccatcgt ctggccgggt gttggcgttt ttggggtgaa aaatttaaat atttcgatac
     2221 caaagaggac gcgcaggcct tctatgacga aacagctttc atgttggcca atcagatggc
     2281 cgcgcccaac agcccacaat ggttcaacac gggcttacac tttgcctacg gaatcaacgg
     2341 ccccagtcaa ggccacttct acgtcgatcc caaaacaaaa gagctcgcca aatcaacaag
     2401 tgcctacgaa cgtcctcagc cacacgcgtg tttcatccaa tccatctcgg atgacctcgt
     2461 caacgacggc ggcatcatgg acctctggat caaagaagcc cgccttttca aatatggttc
     2521 cggtacaggc accaattttt cttctctgcg cgctaaaggc gagagcctgt caggcggcgg
     2581 caggtcttcc ggtctcatga gttttttaaa aatcggcgat gccgcggcag gggctattaa
     2641 atctggcggc acaacacgtc gcgcggccaa aatggtcatt gtcgacgtag accaccccga
     2701 cgtgcaggag tttgtcaact ggaaggtcgt cgaagaacaa aaggtcgcct gcctcgtcac
     2761 cggatcacaa gtcatccgaa aggccctcag tcaaatcatg aaggcctgtc aaaacaagga
     2821 actcaaaaac gaccgcttcg atccgttgaa aaacaaagaa ttggggttgg ccgttcgtga
     2881 tgccagatcg caacaggtgc cacaaaatta cattcttcgc gtgattcagt ttgccagaca
     2941 gggttttacg agccttgatt ttgccgaata caacgtcgac tggaattccg atgcgtacgc
     3001 gacggtatcg ggtcagaatt ccaacaacac ggtgcgcgtg aacaacgact tcatgaatgc
     3061 cgttcgagaa aatgcggatt tttccctcaa gggaagaatc accgatgaga acatcaaaac
     3121 aatcaaggcc aaggaattgt gggaacagat ctgttccgcc gcctggaact gtgctgaccc
     3181 tggtttacaa ttcgacacag ccatcaatga atggcacacc tgtccccagg acggccggat
     3241 caatgccagc aacccctgta gcgagtacat gtttttagac gataccgcgt gcaacttggc
     3301 cagcctgaat ctggtcagtt tttacgacac ccaaaaaggg acttttcaga tcagggattt
     3361 cgcacacgca acccgcctct ggaccatcat tctggaaatc tcggtcctca tggcgcaatt
     3421 tccctcacag ctcattgcac aaaaaagttt tgatttccga accctcggcc tgggctatgc
     3481 caacctgggt gccctgctca tgcgcatggg acttgcctac ggcagtaaag aagccgttgc
     3541 catcacaggc gccatcacag ccttgctcac cggcgaggcc tatgccacct ccgccgaaat
     3601 ggccaaagag cttggtccct tccaaaaata ttaccgcaac caggaagaca tgttgcgcgt
     3661 gattcgcaat caccgccgtg tggcctacga tgccccctcc gacgagtacg aaatgctcac
     3721 catattgcct cagggcatcg atcagaaaaa ctgtccgccc gatctgttgc aggcagccaa
     3781 agagtcctgg gaccacgcct tggaaggggg agaaaaatac ggtttccgca acgccatggt
     3841 ttctgtcatc gcacccacgg gcaccattgg gctggtcatg gactgcgaca ccaccggcat
     3901 cgaaccggac ttcgccttgg tcaagttcaa aaaactcgct ggtggcggtt atttcaaaat
     3961 catcaacgaa tctgttcctc ccgcactgaa aaaattgggc tatacagaca accagattca
     4021 ggacatcgtc aattattgcc gtggcacagg gaccctcaaa tccgcaccag ccatcaatca
     4081 caaaacactg acggaaaaag gcttcactgc ggacgctctg gaaaaactcg aaatcgcact
     4141 cacgcaggcc ttcgacatca cgttcgtgtt caacaaattc actttgggcg agaccttttt
     4201 caaacgcgat ctcaaacttg acccaaaaca gtataacgaa cccagcttca atctgctcaa
     4261 acaactgggg ttcacggatc aggatattga acgggccaac aactactgtt gtggcaccat
     4321 gactgtagag aaggcgcccc atattgatcc caagcattat cccgttttcg actgtgccag
     4381 caagtgtggt cgttacggca cgcgcttcat ttcggcggat gctcacgtcg agatgatggc
     4441 cgcggcacaa cctttcatct cgggagccat ttccaaaacc atcaacatgc ccaacgatgc
     4501 gaccatcgaa gacatcagcc gcatttattt taaatcctgg ggactcatgc tcaaggccaa
     4561 cgccatttat cgcgatggtt ccaagct
//
LOCUS       DATDUN010000404         1117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_269651, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000404 DATDUN010000000
VERSION     DATDUN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269651"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VJC18_05640"
     CDS             complement(<1..334)
                     /locus_tag="VJC18_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLD44898.1"
                     /translation="MTHRTYKKTFLRPFVLSSFRPFVPRPSSLVLSSFRSQGFTLLEA
                     LLSIVIIGAGLIGVLYVFTAGTRGSLVANQTIIASNLAREKLDQIVASRASAGYPATI
                     ATNFSDGVL"
     gene            complement(354..812)
                     /locus_tag="VJC18_05645"
     CDS             complement(354..812)
                     /locus_tag="VJC18_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLD44899.1"
                     /translation="MAQRGFTIIELVVVIAILGVLSVAAVTMTFDFKRSYVEVAAQKT
                     RSDIEHARSLAMTKKGTIYGAFFNDATDSYTVYQTAIATPVEDPLTKQNLIENFSKWS
                     GVSITGGDYTVEFNQLGAPTVGGGGGVQLTDGTNTKIISVTAVTGKVSVE"
     gene            complement(815..>1117)
                     /locus_tag="VJC18_05650"
     CDS             complement(815..>1117)
                     /locus_tag="VJC18_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44900.1"
                     /translation="ARDAVSSAVQTALSLYAASQISQGAAESYPADLDGEDDGIAADR
                     EHPLFDIILKGGVIKSWTKQDDNCYQYTGGAGTDEYQYDSTAPDPVGTFVYVSAGC"
BASE COUNT          281 a          279 c          297 g          260 t
ORIGIN      
        1 ctaaaacacc gtcggaaaaa tttgttgcga tcgttgccgg gtaacccgca ctggccctgc
       61 tggccacaat ctgatcgagc ttctctctgg ccaaattcga tgcgatgatc gtctgattgg
      121 cgaccagaga gccacgcgtg ccggcagtga acacatacaa gacaccaatg agtcccgcgc
      181 cgatgatcac aatggagagc agtgcttcga ggagggtgaa gccttgggag cgaaaggacg
      241 aaaggacgag ggacgaggga cgagggacga aaggacgaaa ggacgaaagg acgaaaggac
      301 gaagaaaggt ttttttataa gtccgatggg tcatataggt ccaatcattc tcattattcc
      361 acagaaacct tccctgtcac cgctgtgaca gaaattattt tggtgttcgt gccatcagtg
      421 agctgaacgc cccctcctcc ccccaccgtg ggagcaccca gttgattgaa ttctacggta
      481 taatcgccac ccgtgatcga gacgccagac cattttgaaa aattctcgat gagattttgt
      541 ttggtaaggg ggtcttctac aggcgttgcg atggccgttt gataaacggt gtaagaatcg
      601 gttgcgtcat taaaaaaggc accgtaaatg gtgccttttt tcgtcatggc caagcttcgt
      661 gcgtgttcga tgtcggaacg tgtcttttgg gcggcaactt ctacgtagga tcgcttgaaa
      721 tcgaaggtca tcgttactgc ggcaacagat aagactccca aaatggctat cacgacaacc
      781 agttcaatga ttgtgaatcc ccgttgggcc ataatcaaca accagcggaa acgtaaacga
      841 aagtgccgac ggggtctgga gctgtgctgt catactggta ttcgtcagtg ccagcaccgc
      901 ccgtgtattg gtaacaatta tcatcctgct tggtccagct tttgatcacg ccacccttga
      961 ggatgatgtc gaacaaagga tgttctctgt cagccgctat gccatcgtct tcaccatcaa
     1021 gatctgcagg atagctctct gccgccccct gtgaaatctg gctggccgcg tataacgata
     1081 aggccgtttg caccgctgaa gacaccgcgt cgcgggc
//
LOCUS       DATDUN010000405         1491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_436395, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000405 DATDUN010000000
VERSION     DATDUN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436395"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..>1491
                     /locus_tag="VJC18_05655"
     CDS             168..>1491
                     /locus_tag="VJC18_05655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLD44901.1"
                     /translation="MKAKINHLSPYQHLGVIVTVHLLLSLPGLWVPYFNIDELTNALY
                     ARFINAGMVGLKDFLGSTYLLTHYLYALVYSFTEKNNMLPMHIVHTFWQAGTIGALYG
                     AGSLLGNKKTGLWAAWFYCVFAHGFLSKDFYTPSAESLSLFPAVLCAIFVIRAFKNRA
                     TLDYLLVGVFAAIAASFKAPMACVLLAAVLAFLIGGGFCLRGFLALGFGFVCVIILPV
                     CLVEPFGSGFGLMRDKFTETNTLYIQAYEGFSFLYWFIKFVIRTTLVLSASFAMTVFA
                     VYGIRPVFRLGRFKSDDFRHTLFLFLWLVLLWFTIALGKRVFYHYFVFLLGPLSLLAA
                     EGVRFWDERQPGAAKRVSRGKIWLLATLGHVRKHMVLLMALPASLFFIEAACNFSTMP
                     NDVTSSIEYVKQHTSGADRIYIWGNVPQLYFYSERLPSTAYFWSDVLAG"
BASE COUNT          291 a          323 c          420 g          457 t
ORIGIN      
        1 gatcgagttc ttttggcgaa gatgcaggat aagtcctgct tgatggccag ggagtacacg
       61 tgggagaaag tggttgatgc ctatgtggac ctgtgtgaag gacgaaacaa ttgagcaatg
      121 agcagttggc aacggcaaca attgcatcaa tggcagtaat tgctacaatg aaagctaaaa
      181 tcaatcacct atccccgtat cagcatttgg gtgttatcgt gacagttcac ctgttgttgt
      241 ctttgcccgg tctgtgggtg ccttatttca atatcgatga gttgaccaac gcgttgtacg
      301 cgagattcat caatgcaggg atggttggcc tgaaggattt tttgggaagt acctacctgc
      361 tgacgcatta cctgtatgcc ctggtttatt cgtttacaga aaagaacaat atgcttccga
      421 tgcatatcgt gcacactttc tggcaggcgg ggacgatcgg ggctctgtat ggggctgggt
      481 cattgttagg aaataaaaaa acgggactgt gggcggcgtg gttttattgt gtgttcgcgc
      541 acggttttct gagcaaagat ttttacacgc ccagtgcgga gagcctgtca ctgttccctg
      601 cggtgctgtg tgccattttt gtcatcaggg cgttcaagaa tcgggcaacg cttgattatc
      661 tgttggtggg tgtttttgcg gccatcgccg cgtccttcaa agcccccatg gcgtgtgtgt
      721 tgctggcggc cgtgctcgcg tttttgatag ggggcggatt ttgtctccga ggttttttgg
      781 cgttggggtt tggttttgtg tgcgtgatta tcctgccggt ttgtctcgtg gagcctttcg
      841 gatcaggttt tggtctcatg cgcgacaagt ttaccgaaac caatacactt tacatccagg
      901 cctatgaagg tttttctttt ctgtattggt tcatcaagtt tgtcatccga accaccctgg
      961 tgttgtctgc gtcgtttgcc atgacagtgt ttgctgtcta tggtatcagg cccgtgtttc
     1021 ggcttggtcg tttcaagtct gacgatttcc gacacactct ctttttgttt ctgtggctgg
     1081 ttttgctttg gtttaccatc gctctgggca aacgggtatt ttatcattat tttgtgtttt
     1141 tactgggccc gctatcgtta ctggcggcgg aaggggtccg gttttgggac gagcgacaac
     1201 ccggggccgc aaagcgagtg agtcgcggaa aaatctggtt gttggcaacc ctggggcatg
     1261 tacgcaaaca catggtgttg ttgatggccc tgccagccag cctctttttt atcgaggccg
     1321 cgtgcaattt ttcgaccatg cccaacgatg tgaccagttc cattgagtac gtcaaacagc
     1381 acacctcagg ggcagaccgt atctatattt ggggcaatgt gccacaactc tatttttatt
     1441 cggagcgtct gccatcaaca gcgtatttct ggagcgatgt gttggcagga a
//
LOCUS       DATDUN010000406         1133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_436475, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000406 DATDUN010000000
VERSION     DATDUN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436475"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1087
                     /locus_tag="VJC18_05660"
     CDS             329..1087
                     /locus_tag="VJC18_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLD44902.1"
                     /translation="MKDKTSYIKQLAHDIRSPLATLQMAAGDVQMTLAGNDELSDSLT
                     LINLSVNRLMGMAENLLCRLCDQTILEQSIDVHEVIDKLMAEYGHTKQLWQITFTNQY
                     FDSSLKVSGAPERLMQALGNIIQNAIEAMNYCGEIFITTMKKDGQVVIIIKDTGPGMS
                     DQIKRNIFTEGYTTKKEGHGIGLGVVSNVINELNGTVKVFSKPGQGCIFHLTLPLAGA
                     SPEADASIETIDLPKTVMCPLTNKSGERWIKESL"
BASE COUNT          336 a          229 c          265 g          303 t
ORIGIN      
        1 gtttcaagct cactgatgtt gtcaccaatt ttatttaata gttcatcaga aacatgatcg
       61 aaagaagttg agggtgaatg gcgaataaaa aactgttcat ttttgaggga taagaacttc
      121 tgtttttctt caagatcaat gctcacatgt gtgatctctt ttgccagagt cgacaaatta
      181 tgatccccaa tatcgttttg acgaatgccc gtatgacgga ggtgcgagtt gtaagggttt
      241 gtgcaatatt gtttaataaa gtcatgatgt taatattcct gtcatcatat ttacacgaat
      301 tggcatgtaa actgcttatg aaaacggcat gaaagacaaa acatcataca tcaaacaact
      361 ggcgcacgat atcagatcac ccctggcaac acttcagatg gccgctggtg atgttcaaat
      421 gaccttggcg ggcaatgacg agttgagtga ttcgctgacg ctgatcaatt tgagtgtcaa
      481 tcgccttatg gggatggccg aaaatctttt gtgcaggtta tgtgatcaaa ctatcttgga
      541 acaatccatt gatgtgcacg aggtcattga taaactgatg gctgagtatg gccatacaaa
      601 acagctctgg cagatcacat ttaccaatca atattttgat tcttcgctta aagtctcggg
      661 agccccagag cgtttgatgc aggcacttgg caatatcatc cagaacgcca ttgaggccat
      721 gaactattgc ggagagattt tcatcaccac catgaagaaa gacggacagg tagtcatcat
      781 cataaaggac acgggtcctg gcatgagtga ccagatcaaa agaaatattt ttacagaagg
      841 gtacaccacc aagaaagagg ggcatggtat cggtctgggt gtggtcagca atgtcatcaa
      901 tgagctcaat ggaacggtaa aggtcttctc aaaacccggg cagggttgca tttttcatct
      961 gaccttgccg cttgctgggg cctctccaga agcagacgca tcaatcgaaa cgatcgacct
     1021 gcccaaaacc gtcatgtgtc ccctcacgaa caagagtggc gagcgatgga taaaagaatc
     1081 attgtgatcc ctgtggacag taaaatgatg gtggcatttt taaccttgag aat
//
LOCUS       DATDUN010000407         3274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_354087, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000407 DATDUN010000000
VERSION     DATDUN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354087"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1003
                     /locus_tag="VJC18_05665"
     CDS             68..1003
                     /locus_tag="VJC18_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLD44903.1"
                     /translation="MGIESNNLSQKRKTTASKTKLSQLIHLHEGYARSQDGTRIWYQS
                     QGQGTPIIFCNGLGCSWAFWKHVHAHFAKKHQVIVFDWRCHGKSEHITDEDKLTIDTL
                     AEDMHAVLKKLTINKAIIAGYSMGTQVLYRFWEKYPKKVLALIPCCGTYSRPMDTFYN
                     SKTSKYIFAGVYIFNHFFPRLSNSLGHLLSKNPLWWQMGTALEMLNPGLADRKVLKEY
                     IEHFTAMDSVMLTALAQSMQDYNAEPILKRIHVPTLIIAAENDRFTPVWISKKMHHLI
                     PQSELIIIKKATHVALVEQPALINLRIEKFLQERL"
     gene            1102..2262
                     /locus_tag="VJC18_05670"
     CDS             1102..2262
                     /locus_tag="VJC18_05670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLD44904.1"
                     /translation="MKVLVSILCAGLILATVSCSKIQTQNTAQDIFDLPKAKQGDLIA
                     KVNDTEIHQGLLDILKELNPRVEQQLSNPMTRKKIITSLIDQQLLYQEATKRNLASQE
                     DVIMKYLINRHAIITNALLQQEVEQAMTKAYEEKKDSQFTKLKVALIAANFKKDEPAK
                     DKKEQPAKPTDTEVANALERIQKIKARIDKGEDFAKMAQAESDDKLTSKKGGDAGKIS
                     KDDKRFERLGYGPLVTAAFAMKKDQVSDPIKTDKGYFLIKVTDDPSVVPYEEAKRVLG
                     FELQNKIKDDLLAKLKNGAKIEYATVDTPEPKKEDSANPLQNQLNMIQSGIQQKAQPQ
                     ANDPHQTLPEPKKEPGQASLQDKLNMIQSGIHKQTPPEDEKNGATPKKDDAH"
     gene            2265..>3274
                     /gene="purM"
                     /locus_tag="VJC18_05675"
     CDS             2265..>3274
                     /gene="purM"
                     /locus_tag="VJC18_05675"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019520133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HLD44905.1"
                     /translation="MNKPITYKDSGVDISAGDDLVDLIQVSAKKTQRPEVLGGLGSFA
                     GLFSLEKCSLKKPVLVASTDGVGTKLKLALDDQHIQNLGQDLVAMCVNDLICCGAEPL
                     FFLDYYATGALNTAQAALVIQNIASCLENINCTLLGGETAEMPGLYAKGDFDLAGFAV
                     GIVDRDRIITGEQVKEGDVIIGLTSSGVHSNGYSLVRKIIEQKKLDLHTKPAGWHAPL
                     GQVLLEPTRIYVNPVLAFIKQYPVQAMAHITGGGLVENLPRVFPDTFQAQLFKNKIPT
                     PAVFRYLQEQGNVPEPEMWRVFNMGIGFALIVDASLKAEAIKFFEQQNVPAFEIGVIT
                     RQ"
BASE COUNT          984 a          884 c          695 g          711 t
ORIGIN      
        1 agaaagagtt gcttaacaca agattatgat tgtctatttg tgataaatct aataatttag
       61 cgacgtcatg ggcattgaat ccaacaatct gtcacaaaaa cgcaaaacca cggccagtaa
      121 aaccaagtta tcacagctca ttcatttgca tgagggctac gccagaagcc aggacggcac
      181 ccgaatctgg tatcaaagtc agggccaggg gaccccgatc atcttctgta acggtctggg
      241 ctgttcctgg gccttttgga aacatgtgca cgcgcatttt gcaaaaaaac accaggtcat
      301 tgttttcgac tggcgttgtc acggcaaatc cgaacacatt acggacgaag acaaactcac
      361 tatcgatacc ctggccgaag acatgcacgc cgtgctcaaa aaactcacca tcaacaaagc
      421 catcatcgcc ggctattcca tgggcactca ggtcttgtat cgcttttggg aaaaataccc
      481 caaaaaggtc ctggccctta ttccctgttg cggcacctac agccgcccca tggacacttt
      541 ttacaacagc aaaacctcca aatacatttt tgccggggtt tacattttca atcatttttt
      601 tccgcgtctt tcaaacagtc tgggtcacct gctgtccaaa aaccccctct ggtggcaaat
      661 gggcacggcc cttgagatgc tcaaccccgg cctcgcagac aggaaagtgc tcaaagaata
      721 catcgagcat tttacagcca tggattctgt catgctcaca gccttggccc agagcatgca
      781 ggattacaac gccgagccca tcctcaaaag gattcatgtc cccaccctca ttatcgccgc
      841 cgaaaatgat cgtttcaccc ccgtgtggat ttcaaaaaaa atgcatcacc ttatccccca
      901 atcagaattg atcatcatca aaaaggccac acacgtggcc ctcgttgaac aaccagccct
      961 gattaacctg aggatcgaaa aatttttgca ggaaagactg tgactcgata ttcatgtttc
     1021 cacttgcctt aaaacacact catgtttata atcccacgcc acaagtttcg ttcaatataa
     1081 ccaaactcat tggaggtttt catgaaagtt ttagtttcta ttctatgcgc gggtttaatc
     1141 ttggccactg tgtcctgttc aaaaatacag acacaaaaca cggcgcagga catttttgat
     1201 ctgcccaaag ccaaacaggg tgatttgatc gccaaggtca atgacacaga aatccatcaa
     1261 gggcttctcg atatcctcaa ggaactcaac cccagagttg aacagcaact cagcaacccc
     1321 atgacccgca aaaaaatcat cacgtcgctc atcgaccaac aactcctgta tcaagaagcc
     1381 accaaacgca acttggccag ccaggaagac gtcatcatga agtacctcat caacaggcac
     1441 gccatcatca ccaatgccct cctgcaacaa gaggtggaac aagccatgac caaggcgtac
     1501 gaggaaaaga aagattccca gttcaccaaa ctcaaggtcg ctctcatcgc ggcaaacttt
     1561 aaaaaagatg agcctgcaaa ggacaaaaag gaacaaccag ccaaacccac cgacacagaa
     1621 gtcgccaatg ccctcgagcg cattcaaaaa ataaaggccc gtattgacaa aggtgaggat
     1681 tttgccaaaa tggcgcaggc agaatctgat gacaaactca caagcaaaaa aggtggcgat
     1741 gccggcaaaa tatcaaaaga cgacaaacga ttcgagagat tgggttacgg cccactcgtg
     1801 accgccgcct ttgccatgaa aaaagaccag gtctccgatc ccatcaaaac agacaaaggg
     1861 tacttcctca tcaaagtcac tgacgatcca tcagtcgttc cctacgaaga agccaagagg
     1921 gtcttgggtt ttgaactgca aaataaaatc aaagacgatt tattggccaa actcaagaat
     1981 ggtgctaaga tcgaatatgc cacggtggac acgcccgaac ccaaaaaaga agatagtgca
     2041 aatcccctcc agaaccaact taatatgatc cagagcggca ttcagcaaaa ggcacagccc
     2101 caggctaacg acccccatca aacattacct gaacccaaga aagaacccgg acaagcttct
     2161 ttgcaggata aactcaatat gattcaaagt ggcattcaca aacaaacgcc ccctgaagac
     2221 gagaaaaatg gggcgacacc caaaaaggac gacgcccatt aatcatgaac aagcccatca
     2281 cctacaagga ctccggcgtc gatatctcag caggagatga tttggtggac ctcattcagg
     2341 tctccgccaa aaagacgcaa aggcctgaag tgctgggagg gcttggctct tttgctggtc
     2401 tgttctccct ggagaaatgt tccctcaaaa aacccgtact ggttgcctcg acagacggag
     2461 ttggcaccaa actcaaattg gccctcgatg accagcatat tcagaatctg ggtcaggatt
     2521 tggtggccat gtgtgtcaac gacctcatct gttgtggggc cgagccactg ttttttttgg
     2581 attattatgc cacaggggca ctgaacacag cgcaggccgc gctggttatc cagaatatcg
     2641 cctcctgcct tgaaaacatc aactgcacat tgttgggggg tgaaaccgca gagatgcccg
     2701 gtctatatgc caaaggtgat tttgacttgg ccggttttgc cgtgggcatc gttgatcgtg
     2761 atcgcatcat cactggtgaa caagtcaaag agggtgatgt catcattggg ctgacctcca
     2821 gtggtgtaca ttcaaacggt tactcactgg tccgcaaaat tattgaacaa aaaaaactcg
     2881 acttgcacac caaaccagcc ggttggcatg cccccttggg acaagttctg ttggagccca
     2941 cacgcatcta cgtcaatccc gtgttggcct tcatcaagca ataccccgtt caggccatgg
     3001 cacacatcac aggtggcggc cttgtggaga atctgccgcg cgtctttcca gatacctttc
     3061 aggcccaact ctttaaaaac aagataccca cccctgctgt gttcagatat ttacaggaac
     3121 aaggcaacgt tccagaaccc gaaatgtggc gcgtgttcaa catgggcatc ggtttcgctc
     3181 tcatagtgga cgcatcactc aaagctgaag ccatcaaatt tttcgaacaa caaaatgtgc
     3241 cggctttcga aatcggggtg atcacaagac aaca
//
LOCUS       DATDUN010000408         1570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_310580, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000408 DATDUN010000000
VERSION     DATDUN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310580"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1570)
                     /locus_tag="VJC18_05680"
     CDS             complement(<1..>1570)
                     /locus_tag="VJC18_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44906.1"
                     /translation="NMFMWACVGELAPDEAYQKYLGAMTEKPATGIEPDLIERIHQVS
                     GDAVYAQQRPMKELLDARQVPSGPFVNSQIVNEFYRNFYFSILHRMGMYVLEQLSSID
                     AAKLFAQQLQQALAQDEVGRDMASWFGSLCLLEVRVSGPIMAEDYFNFTRLGPTAAVR
                     VYKKLTKYLSFGDAAFGVATEQLLQRLDDRIENRHYLSRFAINLLNDVFLYRRLVPNY
                     KDEVVRNYPYDSAYDAYLNKDYQKIYDLLSVATGEKGNLIKLISKGEGIDVEKVIDLW
                     KGAVKERPDAVGLAQGYAEFLMEQKQYAQAREVITAWLKISQPKDLQLVHSMNLIAKS
                     YLLEGEYQKGYDAIAPHLEVGQNYSFLLAGQLLAKLNRFDEAKLIFLKAIDRYPGYFK
                     TVMPYVKICWEHGKFEEAAQLLSQHAKLIRIEDWRWIVGKEFAEVFAELPLAKAQQAI
                     SFLVTNNISGYALRELAIALKNKEHYELALDVLDKVHVSGLGQVQADLDAYFILRKTK
                     SDSEALTWLRGRLGG"
BASE COUNT          392 a          444 c          335 g          399 t
ORIGIN      
        1 ccacccaggc gcccacgcaa ccaagtcaag gcctcgctgt ctgacttggt cttgcgcaga
       61 atgaaatagg catccagatc ggcctgcacc tgtcccaaac cggacacatg cactttatcg
      121 agaacatcga gggccaactc gtagtgttcc ttgttcttca gggcaatagc gagctccctg
      181 agggcataac cagaaatatt attggtcacc agaaacgaaa tcgcctgttg cgccttggcc
      241 aacggcaatt cggcaaagac ctcggcaaac tctttcccca caatccatcg ccaatcttca
      301 atgcgaatca attttgcgtg ttgcgacaac aactgcgcgg cctcctcgaa cttcccgtgc
      361 tcccaacaga tcttcacata aggcatcaca gttttaaaat aaccggggta tcgatcaatg
      421 gccttgagaa aaatcagctt tgcctcgtca aaacgattca acttggccag aagttggcct
      481 gccaggagaa acgaatagtt ctgcccgact tccaaatggg gtgcaatggc gtcgtaccct
      541 ttttgatatt ccccctccag gagatatgat ttggcaatca ggttcattga atgaaccagt
      601 tgcagatcct tgggttgact gatcttgagc cacgcagtga tcacctcacg tgcctgggca
      661 tactgctttt gctccattaa aaattccgca tagccctgcg ccaaacccac agcatctggt
      721 ctttctttga cagcgccctt ccacaaatca atgaccttct caacgtcaat accctctcct
      781 ttactgatga gctttatcaa gttgcctttt tctcctgtgg caacagaaag cagatcgtaa
      841 atcttttggt agtctttgtt cagataagca tcgtaggcgc tgtcgtaggg ataatttctg
      901 acaacctcgt ccttataatt tggaacaagc cttcggtaga gaaagacatc gttgagcaaa
      961 ttaatcgcga atcgcgacaa gtagtgcctg ttctcaatgc ggtcatctaa tcgctgtagc
     1021 agttgctccg tggctacacc aaacgcggca tctccaaaac tcaaatattt ggtcagcttt
     1081 ttgtataccc tcactgcggc tgttggtccc aaacgagtaa agttaaaata atcctctgcc
     1141 atgatcggac cagacaccct gacttccaag aggcaaagac tgccgaacca actggccata
     1201 tcgcgcccca cttcgtcttg tgccagtgcc tgttgcagtt gttgcgcaaa cagcttggcc
     1261 gcatcgatac tgctcaactg ttccagcaca tacatcccca tccggtgcag gatggaaaaa
     1321 tagaagttcc tgtaaaattc attcacgatt tgagaattca cgaacgggcc agatggcact
     1381 tgcctggcgt ccagcaattc tttcatcggt ctctgttgtg cgtagaccgc atcaccgctg
     1441 acctggtgga ttcgctcgat cagatcaggc tcgatccccg tcgccggttt ttctgtcatc
     1501 gcccccagat atttctgata cgcttcgtca ggggccaact ctcccacaca ggcccacatg
     1561 aacatattga
//
LOCUS       DATDUN010000409         1585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_145186, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000409 DATDUN010000000
VERSION     DATDUN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145186"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1585
                     /locus_tag="VJC18_05685"
     CDS             <1..>1585
                     /locus_tag="VJC18_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44907.1"
                     /translation="LIVPPHPTRNQHIWEFTLSSDAAFRATGMPLIFTTSQQIATLIS
                     HSAERLPTGGLVSHKFQQKIHALLPHVLAKTGNRQDLYDRVVNYPKNQPECIDPVTEE
                     DLYRNYFFMNGPNVLAKTRWALGVNASGPVQDTSTNSLWGGSLCQNTLVRQKCEISED
                     CTNSSSSCQAGYCDAFDPQTDRWDVLREISYETVPSHPVFPSLNSDFSLNGGVSCHYG
                     STAQAMIEVATHNQACNPYGPDVNEVVGTEELLYRAVAAGLIDLLTITEDEWWPGLIT
                     NGSASEDLFFLLTYKSMPTYHAAAPYLRQIMTDRVNGRSLGDQVFELWTEAMGRMVDR
                     GIPEVMASSLNQTAHQVLNLEMFYQASEISLYETHARHFSKRFVESAHPAGFFREGSG
                     IDATYPGMTHWNMGQAYALSRSDPIGPDNRLREGLRRSYYFLNHTVAPEPDGSFVGGF
                     NFAQRTGGGFFQEQYQGAVFLIEDLPEIAVWSRPHAASITDADSLRAFHTFQTNSFET
                     NADRLLESWGIRQDALLWKF"
BASE COUNT          401 a          411 c          389 g          384 t
ORIGIN      
        1 ctcattgtgc cgccgcatcc tacccgaaac cagcatatct gggaattcac gctctcatct
       61 gatgccgctt ttcgggccac gggaatgccc ctcattttca caacatccca gcaaatagca
      121 acacttatca gtcattccgc cgaacgcttg cccacaggag ggctcgtcag tcacaagttt
      181 caacaaaaga tccacgccct tctgccacac gtattagcta agacaggtaa cagacaagac
      241 ctctacgaca gggtggtcaa ctatcccaag aaccagcccg aatgcatcga tcctgtcaca
      301 gaagaagatc tgtaccgaaa ctactttttt atgaatggac caaatgttct ggcaaaaaca
      361 cgttgggccc tgggcgtaaa cgcaagtggg cctgtgcagg atacaagtac caacagttta
      421 tggggaggct ccctttgtca aaacacgctg gttcgccaga aatgtgaaat cagtgaggac
      481 tgcactaaca gcagttcttc ttgtcaggcg gggtattgtg acgcatttga cccccaaaca
      541 gatcgatggg acgtcttgcg tgaaatcagt tacgaaactg ttccaagcca tccagtattc
      601 ccctcgctca attcggattt ttccctcaac ggcggtgtct cgtgccacta tggaagcaca
      661 gcacaagcca tgatcgaggt tgccacacac aatcaggcct gtaatcctta tgggcctgat
      721 gtcaatgagg ttgtgggcac agaagagttg ctataccgtg cggtagcggc cggactgatc
      781 gacctgctga ctatcaccga ggacgaatgg tggcctggac tcatcacgaa tggttcggca
      841 tcggaagatt tgtttttctt gctaacatac aaatccatgc ccacatacca tgcggctgcg
      901 ccatatctgc gccagatcat gaccgaccgt gtcaatggac gttcgttggg tgatcaggtc
      961 tttgaactct ggaccgaggc aatgggacgc atggtcgatc gggggatacc cgaggtcatg
     1021 gcctcgagtc ttaatcaaac agcgcatcaa gtgttgaatc tggagatgtt ttatcaggca
     1081 tcagaaatca gtctctacga aacacatgcc agacattttt ccaaacggtt tgttgaatcc
     1141 gctcacccgg caggattttt cagggaaggg agcggcattg atgccaccta tcccggcatg
     1201 acacattgga atatgggtca agcctatgcc ctgagtcgaa gcgatccgat aggtcccgat
     1261 aatcgtttac gggagggact acggcgttcc tattattttc tgaatcatac tgtcgcccct
     1321 gaacctgacg gttcatttgt gggcggattc aattttgcac agagaacggg cggtggtttt
     1381 ttccaagaac aataccaagg agctgtgttt ttgatcgaag atctgcccga aatcgcggtg
     1441 tggtcacggc cccatgcagc atccattacc gacgccgatt cattacgggc cttccacacg
     1501 tttcaaacaa attcttttga aaccaatgcc gatcgattgc tggaatcatg gggcatcaga
     1561 caggatgctc tgttgtggaa atttg
//
LOCUS       DATDUN010000410         2419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_23778, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000410 DATDUN010000000
VERSION     DATDUN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23778"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..579
                     /locus_tag="VJC18_05690"
     CDS             151..579
                     /locus_tag="VJC18_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HLD44908.1"
                     /translation="MPNTLNQRIKRLVQLIGTESFDSQEGLLLKLKEEGFDVTQTTLS
                     RDLHKIGAYKEHGNYVLAKLDYALKRMGRIKTIRFCPPNLIVIKTVAGLAQATGLFID
                     NAKINGVAGTVTGDDTIFVGLSDPEKNVVILSELQRILAE"
     gene            1240..1728
                     /gene="ilvN"
                     /locus_tag="VJC18_05695"
     CDS             1240..1728
                     /gene="ilvN"
                     /locus_tag="VJC18_05695"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HLD44909.1"
                     /translation="MKHTISILVENEFGALARIAGLFSSRGYNIESLTVAPTLDPTVS
                     RMTINTNGDERTIEQIIKQLNKLINVIKIKDVTSEDPIHRLLALIKVNLGHRNKEKLK
                     KIIQPFFGRVVDSDERCSIVQLVAEEEKLQELVEQLTPYGVMELVSTGTVAIQKGKHT
                     IK"
     gene            1739..>2419
                     /locus_tag="VJC18_05700"
     CDS             1739..>2419
                     /locus_tag="VJC18_05700"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HLD44910.1"
                     /translation="MTNQAKNVRCHCGQTKEHHWVSPEGNLILLFSLLLSLVLLHCCP
                     YLSLLGIVWFLFCVYFFRNPKRVSPKEEGVLLSPADGVVIVDELVTEPHFLHIPMRKI
                     SVFMSPFNVHVNRNAVSGVVQDKTELAGRFKAAFDKDASEKNERVAVWLRTKEGHDVV
                     MVQIAGWFARRIVNYLKAGDVVAAGNIFGVIKFGSRVDLYFPDDYRVVVLLKQKVRAG
                     ETLLARKKS"
BASE COUNT          670 a          513 c          575 g          661 t
ORIGIN      
        1 gacgtattgc agatcagcac cgttagtttt taaaaccccc ttaagcatcg cgcgcctgta
       61 tggtcaaaac aaaatgaaaa agcaaaaaca aaatttcatt gccaagtcgt gtttcgatga
      121 ttatcttcta tcaccgcggg aggcgctatt atgccaaaca cactcaatca acgcatcaaa
      181 aggttggtgc aactcatagg cacagaatct ttcgatagtc aggaaggtct gttgctaaaa
      241 ttgaaagaag aaggttttga tgtgacccag accaccttgt cgcgtgattt gcacaaaatt
      301 ggcgcgtaca aagaacatgg taattacgtc ttggccaaac ttgattatgc cctcaaaaga
      361 atgggtcgca taaaaacgat tagattttgt ccacccaacc tcattgtgat taaaaccgtt
      421 gccggactcg ctcaggccac aggattattc atagataacg caaagatcaa tggtgtggct
      481 ggcacagtca ctggcgatga caccatcttt gtggggctgt cggatccaga gaaaaatgtg
      541 gttatcctat cggaattgca gagaattctt gcagaatagc atgttttaat cataattcta
      601 ttgacaatcc gtattatttg cgcatatatg cgcatataag tgttcaaaga aggacacttt
      661 ttatttaggc cgagtttttc tacccgaggg gcttggcatg ttgcgcgtat caaagcgcat
      721 ctcgacttga tccagaaaat cttacaagga ttttccctaa aatttaattt aagttcttgg
      781 ctttgtgacc gcctgttgtt ggtagtttga tcggcataca gtgtcacata atgctgggga
      841 cagagtcttt tctagggaga accagatgga tttcaattct ttaagtgaaa tgtctattca
      901 tagatatgat tctgctacaa cgcagccgat agattcggtc acactgcctg atattgatgt
      961 ggtagccatg ttacaaagtg ccgctgaggg gctagtaaag attttgacag gctcaaaatc
     1021 gacacaacct gagtcggcac aatccgcgtc acttgccgct tccaggtccc aaagcccctt
     1081 catcctaaaa tattggggca aataattttt ttgcgggtaa acgtccaaaa agttttttat
     1141 caaaggccgg agcgttcctc cggctttttt tatgtgcgaa gcagacgccg atgattttct
     1201 tgacggtagc aatacaagac cagtattcac taacgttcca tgaaacacac catttctatt
     1261 ctggtcgaaa acgagtttgg tgccttggca cgcatcgcgg gtctcttctc cagccgtggc
     1321 tacaacattg aatcactcac agtggccccc acactggacc ccacagtgtc tcgcatgacc
     1381 atcaacacca atggtgatga acgaacaatt gagcagatca tcaaacagct caacaaactc
     1441 atcaatgtga tcaagatcaa ggatgtgacc agcgaggatc ccatccatcg cctgttggca
     1501 ctcatcaagg tgaatctggg acaccgcaac aaggaaaaac tcaaaaaaat cattcagcct
     1561 ttttttggac gggtcgttga ctccgatgag cgttgctcta ttgtgcaatt ggtggcagaa
     1621 gaagaaaaat tgcaggaact tgtcgaacaa ctcacaccgt atggcgtcat ggaactggtg
     1681 tcgacaggca cagtggccat tcaaaaaggc aaacacacta tcaagtgaga ggccgatcat
     1741 gaccaatcaa gcaaaaaatg ttcggtgtca ctgtggtcaa accaaagagc atcattgggt
     1801 ttctcccgaa gggaatttga ttcttctttt ttcattattg ctgtcgttgg tgcttctgca
     1861 ctgttgtcct tatctttcct tgttggggat agtgtggttt ttgttttgcg tttatttttt
     1921 cagaaatccc aaacgggtgt cgcccaagga agaaggggtt ttgctctcac ccgccgatgg
     1981 cgtggttatt gtagatgaac tcgttacaga accgcatttt ttacacatcc ccatgcgaaa
     2041 aatcagtgtg ttcatgtcgc cttttaatgt gcacgtgaac cgcaatgctg tgagtggggt
     2101 ggtgcaagac aaaacagagt tggcagggcg attcaaggcg gcgtttgata aagacgcgtc
     2161 cgaaaaaaat gagcgtgtgg ctgtttggct cagaacaaaa gaagggcacg atgtagtgat
     2221 ggtgcagatt gccggttggt ttgcgcgaag gatcgtcaac tatcttaaag cgggagatgt
     2281 tgttgcggca ggcaatattt ttggtgtgat caagtttggc tcgcgggtcg atctgtattt
     2341 tcccgatgat tatcgggtag tggtgttatt aaagcaaaaa gtcagggcgg gagagaccct
     2401 tttggccaga aagaagagt
//
LOCUS       DATDUN010000411         1440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_436585, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000411 DATDUN010000000
VERSION     DATDUN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436585"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VJC18_05705"
     CDS             <1..594
                     /locus_tag="VJC18_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HLD44911.1"
                     /translation="ALLIGRLVLLATYSHNNNLWSGGLGTGAYRVSYINLRLDADGAK
                     GPSPKSSPPQKPPASNSLSALSTPHTAVPADPSPPRQTPSFGTGTSDTPAGGVGAGFG
                     QEGVISETAPNVRAQINKMIRQKLIYPLVAKENGWTGTVRVRLHIENNGTVEEVSVVQ
                     GSGYSVLDRSAVATVKKATPLPFYPETILLDIDYQLE"
     gene            764..>1440
                     /locus_tag="VJC18_05710"
     CDS             764..>1440
                     /locus_tag="VJC18_05710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLD44912.1"
                     /translation="MSFVSVIIPTYNRQGTVLRAVDSVLRQSHRAFELLVVDDGSTDN
                     TREWLALYNDPRLRYHCIPHAGVATARNAGAKEASGEWLCFLDSDDLWHKNKLAEQSR
                     FHTAYPHFLLSQTDDVWIRNSERVNKMKKHSVRAGNLFRQSLRLCLICCSSVMIQKEL
                     FMATGGFDETLPTCEDYDLWLRLLSKHEVGFINKELVTKFGGHQDQLSKSFPVMDRYR
                     IYALEKL"
BASE COUNT          349 a          397 c          353 g          341 t
ORIGIN      
        1 gccctgttga tcgggcgcct cgtcttgctg gctacttatt cacacaataa caacttgtgg
       61 tccggtgggc tgggcacagg tgcgtatcgc gtgtcttaca taaacctgcg ccttgatgcc
      121 gatggcgcaa agggaccgtc accaaagagc tctccccccc aaaaaccacc cgcttcaaat
      181 tcgctgtccg ctctttccac gccccacact gctgttccag cagacccaag ccccccccgc
      241 cagaccccgt cttttggcac cggaacatcc gacacgcctg caggtggcgt tggcgctggc
      301 tttggtcaag agggagtgat ctcggaaaca gcccccaatg tgcgcgccca aatcaataaa
      361 atgatccgac aaaaattgat ttaccctttg gtggcaaagg aaaacggttg gacaggaact
      421 gtccgcgtgc ggcttcacat cgaaaacaat ggcaccgtgg aagaggtcag cgttgtgcag
      481 ggctcgggat atagcgtgct ggacaggtct gccgtggcta cagtgaaaaa agccaccccc
      541 ctgccctttt accctgaaac gattttgttg gatattgatt atcaacttga ataaaggcct
      601 tccaagaacc tttttcggta tttttttggg tgatacggga agctattatt ttgatgcggc
      661 ctgcggagcg agcttgcaag cgaagagcat caaaaaatag cgccccgtag caggacccaa
      721 aaaaattgaa gctgtgagct tttcggacaa gctctcataa catatgtcgt ttgtctctgt
      781 catcatcccc acctacaatc gccagggcac cgtgctgagg gccgttgatt ccgttctgcg
      841 tcaatcacac agggcctttg agcttttggt ggtggatgac ggctctacag acaacacccg
      901 cgaatggctc gcgctctata atgatccccg tctgagatac cattgtatcc cccacgccgg
      961 ggttgccacg gcccgcaatg cgggcgccaa agaggcctca ggagagtggc tgtgtttttt
     1021 ggactctgat gacttgtggc acaaaaacaa attggccgag cagtctcgct ttcacacagc
     1081 ttatccacac ttcctcctct cgcagacaga tgatgtctgg atcaggaatt ccgaacgtgt
     1141 caacaaaatg aaaaaacaca gtgtccgcgc gggcaatctt tttagacaaa gtctgcgctt
     1201 gtgtctcatt tgttgctcca gcgtcatgat ccaaaaagag ctcttcatgg ccacaggagg
     1261 atttgatgaa accttgccaa cctgtgagga ctacgatttg tggctccggc ttctgagtaa
     1321 acacgaggtc ggcttcatca acaaagaatt ggtcaccaag tttggaggac accaagacca
     1381 gctatccaag tcctttcccg tcatggaccg ataccgcatt tatgcccttg aaaaactttt
//
LOCUS       DATDUN010000412         1030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_310673, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000412 DATDUN010000000
VERSION     DATDUN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310673"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1030)
                     /gene="mutL"
                     /locus_tag="VJC18_05715"
     CDS             complement(<1..>1030)
                     /gene="mutL"
                     /locus_tag="VJC18_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HLD44913.1"
                     /translation="PNDLINKIAAGEVVERPASVVKELVENSLDAGATQIDITIRDGG
                     KKLIQVSDDGCGIPHDQLSLAIARHASSKIATFDDIYALKTKGFRGEALASICSVAKV
                     SLASCHGDSEAHEVFVDEGEIVDERVCAHPRGTTVTVKYLFHKTPARLKFLKSSETET
                     THIEREITKLALCHPSVGFCLRQDNKELLKLDRGQTLRERVLAVLGKDLADDIFPFGE
                     QQPKIAVSGYFGHPQIARSQRSFGYFFVNARPVNDKVIWHAVMEAYRDLLMRGKYPVL
                     VLNLQIDEHLIDVNVHPTKAEVRFHQSQQVHQIIKNTLRKFLSEAPWLSRRGEGFSRD
                     VTQDFGVV"
BASE COUNT          243 a          285 c          247 g          255 t
ORIGIN      
        1 ctgaccacac caaaatcctg ggtaacgtca cgggaaaaac cctctcctcg tcgtgaaagc
       61 cagggcgcct ccgataaaaa ttttctgagc gtgtttttga tgatctggtg cacctgttgg
      121 gactgatgaa aacgaacttc agccttggtg gggtgcacgt tcacatcgat gaggtgttcg
      181 tcgatctgga gattgagtac cagcacggga tatttaccac gcatcaacaa atcgcgatag
      241 gcctccatca cggcatgcca aatgacctta tcgttgaccg gtctggcatt cacaaaaaaa
      301 tagccaaagc tgcgttgact acgagcaatt tgcgggtgac caaaataacc gctcaccgca
      361 atcttgggct gttgttcgcc aaagggaaaa atatcatctg ccagatcctt ccccaaaaca
      421 gccagcaccc gttctctgag agtttgccca cgatcaagtt tcaataattc cttgttgtcc
      481 tgcctcaaac aaaatcccac cgaggggtga cacagggcca gtttggtgat ctccctctca
      541 atgtgcgtcg tttcagtttc gcttgatttc aggaatttga gtcgggctgg cgtcttgtga
      601 aacaggtatt tcaccgtcac tgtcgttccc cgtgggtgcg cacagactct ttcatcaaca
      661 atctctccct catccacaaa aacctcatgg gcctcactgt ctccatgaca gcttgccagc
      721 gaaaccttgg ctacagagca aatgctggcc aaggcctctc cccgaaaacc ctttgttttg
      781 agggcataaa tatcgtcgaa ggttgcgatt ttgctcgacg cgtgtcgggc aatggccaac
      841 gagagctgat cgtggggaat cccacagccg tcatcgctca cctgaatgag tttcttgccc
      901 ccatccctga tggtgatgtc tatctgcgtg gcacccgcat cgagggaatt ttccaccagt
      961 tccttgacga cagaggcagg tcgttccacc acctcaccgg cggcgatctt gttgatgagg
     1021 tcattgggga
//
LOCUS       DATDUN010000413         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_145350, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000413 DATDUN010000000
VERSION     DATDUN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145350"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /gene="pth"
                     /locus_tag="VJC18_05720"
     CDS             <1..597
                     /gene="pth"
                     /locus_tag="VJC18_05720"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLD44914.1"
                     /translation="LIVGLGNIGKKYENTRHNFGFLVLDALVHTKKLSFKESSKFQGE
                     WCSHNHSDHEKVFYLKPHTYMNLSGASVYALTHYYKINSQDIFVIYDDVDMSFGSVRI
                     ARSGGAGGHRGIQSIIDVLGTNEIPRMKLGIGRPVHEGQEVADHVLDKFSAEEMKEVA
                     SLQDLSTKAIEAYLSQGIDVTMNRFNKVIRPAPKKVDE"
     gene            819..>2007
                     /locus_tag="VJC18_05725"
     CDS             819..>2007
                     /locus_tag="VJC18_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLD44915.1"
                     /translation="MSIIQKGIKTIIVAVVLLGLSGQVWASYSGNVSLTSLTALNSVS
                     YTEQNTRDKGGTDIVTGDFNGDGFIDMAVSDRNYDSANSQTKLIGRVYVFYGDVFGST
                     GSLSTADVIFEAESTTANQLLGYSLAAVDMDNDGDDELFMTASEYSSNRGVAYVIFGG
                     TTLSGTLALSGISPTSTTVPGVRLTGTNSNERVRNVANAGDINGDGYDDLLIGKQKNT
                     SGTTTGTYGTVSLIYGSSTFTTDFTAATYTVASVADATINGYATGNELEFCASAGDLD
                     NDGFGDFFVGSEKYSSNKGIAYLFYGNALLTGTISTSAANANFVAAASSNKAGRAMAG
                     AGDINGDGFGDIVLGAYNATGSTSSSGAAYIFNGSCPRFSGSYTLNSTMATAYLKGTA
                     SADY"
BASE COUNT          517 a          441 c          534 g          515 t
ORIGIN      
        1 ctcattgtcg gtctgggtaa catcggtaag aaatacgaaa acacaaggca taattttggt
       61 tttttggtcc tcgatgcact tgttcacaca aaaaaacttt cattcaaaga gtcctcaaaa
      121 tttcagggtg agtggtgttc gcacaatcat tcggaccatg aaaaagtttt ttacctcaaa
      181 ccacacacct acatgaacct gagcggggct tccgtgtacg ccctgactca ttactacaaa
      241 atcaattcgc aggacatttt tgtgatctac gatgatgtgg acatgtcctt tggttccgtg
      301 cgcattgccc gcagtggcgg cgcggggggg cacaggggca ttcaatcgat catcgatgtg
      361 ttgggcacca acgagatccc gcgaatgaag ctggggattg ggcgtcccgt gcacgagggg
      421 caagaggtgg ccgatcacgt gctcgacaaa ttcagcgccg aagagatgaa ggaggtggcc
      481 agcctgcaag acctgtcaac aaaggccatc gaggcttatt tatcacaggg cattgatgtg
      541 acgatgaacc ggtttaacaa agtcatacgg cctgctccga aaaaagttga tgagtagagc
      601 cttgtgaaga aagctcttcc attttgtaac gactgacaac aatcctggtg tgaatctttt
      661 ttacgggcgt ccacacaatt gtggacgttt tttttattgg ggggctatat ttgtttgttc
      721 ttaacgataa aaggcactaa taaacaaaga atcaagcaaa tgagtttttt tgagcaccag
      781 aaaatcatca tcaacacata acagcaagga gcaagacaat gagcataata caaaaaggaa
      841 taaaaacaat tattgtggca gtggttttac ttggcctttc gggtcaggtt tgggccagtt
      901 attcgggcaa tgtgagtctg actagtctga cggccttgaa tagtgtcagc tacaccgaac
      961 agaacacacg tgataaaggt ggtactgata tcgtgacagg cgatttcaat ggggatggct
     1021 tcattgatat ggctgtgtcc gaccgcaatt atgattcggc gaattctcaa acaaaactca
     1081 tcggtcgtgt gtacgttttt tacggtgacg tttttggatc aacgggaagc ctgtcgacag
     1141 ctgatgtgat ttttgaagct gagtccacga ctgccaatca acttttaggg tacagtttgg
     1201 ccgctgtgga tatggacaat gatggtgacg atgaactctt catgaccgct tccgaataca
     1261 gttccaaccg cggtgtggcc tatgtgatct ttggagggac gacgctttca ggcacgttgg
     1321 ccttgagcgg gatttccccc acgtcaacaa ctgttccagg cgttcgcttg acaggaacca
     1381 atagcaacga gcgtgttcgt aatgtggcca acgcgggtga tatcaacggc gatggttatg
     1441 atgatttgtt gattggtaaa cagaaaaaca cctcggggac cacaacgggc acctatggca
     1501 ctgtgagtct gatttatggc tcgtctactt ttaccacgga ttttacagcc gcgacctata
     1561 cggtagcttc tgtggctgac gcgaccatca atgggtacgc gacgggcaat gagcttgagt
     1621 tttgtgcctc cgcgggtgat ttggataacg atgggtttgg tgattttttt gtggggagcg
     1681 aaaaatattc atccaacaaa ggtattgcct acctctttta cggcaacgcg cttttgacag
     1741 ggacgatctc tacgagcgcc gccaacgcta attttgtagc cgcagcgagc tccaataaag
     1801 cgggtcgggc catggcgggc gcgggggaca tcaacggaga tggctttggg gacatcgtgc
     1861 tgggcgccta caatgccacg ggatcgacat cgagttcggg tgccgcctac atttttaacg
     1921 gcagctgtcc gcggtttagc gggagctata cgctaaattc cacgatggcc acagcatatt
     1981 taaaaggcac agccagcgct gattatt
//
LOCUS       DATDUN010000414         1779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_518803, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000414 DATDUN010000000
VERSION     DATDUN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518803"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="deoC"
                     /locus_tag="VJC18_05730"
     CDS             complement(<1..561)
                     /gene="deoC"
                     /locus_tag="VJC18_05730"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HLD44916.1"
                     /translation="MEINNTNIEQLVELITKKVTERLNQTLSPGNPNAPICSSDPSAC
                     NNCGQCVVNKPGAINNLVDTGAARVGSSTGIKQGVDQKIAGMIDHTLLKPDATKDELT
                     KLCVEAKQYGFATVCVNSSNIPFCARLLKGSTVKPIAVVGFPLGAASTQAKVFEAKEA
                     IRNGAEEIDMVIAIGAIKDRDYQLVYE"
     gene            complement(565..834)
                     /locus_tag="VJC18_05735"
     CDS             complement(565..834)
                     /locus_tag="VJC18_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HLD44917.1"
                     /translation="MILCRVIGNVVATIKQPAYQGHKMLIVQPVDEHGNDKGKSFLSF
                     DSVQAGVGDVVLVEQEGNCARQILGKREDPFHSVICGVVDKVNLE"
     gene            complement(831..1181)
                     /locus_tag="VJC18_05740"
     CDS             complement(831..1181)
                     /locus_tag="VJC18_05740"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HLD44918.1"
                     /translation="MKLAKVIGTVVATVKDPGIQNLKILMIQPLDEQYRPDGGPIAAI
                     DTVQAGPQEIVYYTLAKESTLALPNPFAPVDAAITGIVDELTVEDKGIANKQNIFEEV
                     ASAHLSSDGPKETK"
     gene            complement(1284..>1779)
                     /locus_tag="VJC18_05745"
     CDS             complement(1284..>1779)
                     /locus_tag="VJC18_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HLD44919.1"
                     /translation="DEMVKKAPVRVLETHAICPGKYMVLICGEVADVEESLKKGIEIG
                     HDMIVSHMFLPHAHPDLIPAITGTNVIDHFGALGIVETFSIATCVEAADIAAKATDSK
                     LVEVRLANGLGGKGYFMITGALCDVQASMERAKEFVKNTGMLAGCEIIEAPHPEMLAR
                     GVYW"
BASE COUNT          365 a          477 c          454 g          483 t
ORIGIN      
        1 ttcgtaaaca agttggtaat cgcgatcctt gatggccccg atggcgatca ccatgtcgat
       61 ttcttcggcg ccattgcgga tggcctcttt ggcctcaaaa accttggcct gtgtggaggc
      121 ggctcccaac gggaacccta ctacggctat gggcttgacg gttgatccct tgaggagtct
      181 cgcgcagaac gggatgttgc tggagttcac acagacggtg gcaaaaccgt attgtttggc
      241 ttcaacgcag agtttggtca gctcgtcctt ggtggcgtct ggtttgagca gggtgtgatc
      301 aatcatgccc gcgatctttt gatcaacgcc ctgtttgatg cccgttgagg accctacgcg
      361 cgcggcaccg gtatcgacca gattgttgat cgcgcccggt ttgttgacca cacattgtcc
      421 gcaattattg caggcagaag ggtcgctaga gcaaatgggg gcgttggggt ttcccggaga
      481 cagtgtttgg ttcagcctct ccgtaacctt tttggtgatg agttcaacca gttgttcgat
      541 gttggtattg ttgatttcca tattttactc caaattcacc ttatccacca cgccgcaaat
      601 gactgagtga aaagggtctt cccgtttgcc cagtatttgt ctggcgcagt taccttcttg
      661 ctccaccaaa accacatcgc ccacacccgc ttgtacggaa tcaaatgaca agaaggattt
      721 ccccttgtcg tttccgtgtt catcaaccgg ttgcacaatc agcattttgt gtccttggta
      781 ggccggttgc ttgatggtgg cgaccacatt gccaatgact ctgcagagga tcatttggtc
      841 tcctttggtc cgtcggagct caaatgagcg gaggcgacct cttcaaaaat attttgtttg
      901 ttcgcgatcc ctttgtcctc cacagtcaat tcgtccacaa tccctgtgat ggccgcatct
      961 accggcgcga acgggttggg cagggccagt gtggattctt tggccagcgt gtaataaaca
     1021 atctcctgcg ggccagcttg cacagtgtcg atggcggcaa ttggtccgcc gtcaggacga
     1081 tactgttcat caagcggctg gatcatcagt atcttcaggt tctggatgcc ggggtctttg
     1141 acagtggcaa caaccgtgcc gatgacttta gctagtttca taaaaaatag tctctgattg
     1201 ttttaatctg ggcttaaggc tcaaggcaca aggtgtaagg cgtcaaaatc gccctatgcc
     1261 ttacgccttt agccatgcgc cctttaccaa tacactcccc ttgccaacat ctctggatgc
     1321 ggtgcctcga tgatttcgca cccagccaac atacccgtgt ttttgacaaa ttcctttgcc
     1381 cgttccattg aggcctgaac atcgcacaat gcgcccgtga tcataaaata ccctttgccc
     1441 cccaggccgt tggccagtct gacttccacg agtttgctgt cggtcgcctt ggccgcgata
     1501 tcggcggctt caacacaggt tgcgatcgaa aatgtttcaa caatcccgag cgcaccaaaa
     1561 tgatcaatca catttgttcc tgtgatggca gggatgaggt ctgggtgtgc gtgtggcagg
     1621 aacatgtggc ttacgatcat gtcatgtccg atctcgatcc ctttttttag ggattcctcc
     1681 acatcggcca cctcaccgca aatgagcacc atgtatttgc ccgggcagat ggcatgggtt
     1741 tcgagaacgc gcacaggcgc cttcttgacc atctcatcg
//
LOCUS       DATDUN010000415         7121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_354232, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000415 DATDUN010000000
VERSION     DATDUN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354232"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="VJC18_05750"
     CDS             complement(<1..1645)
                     /locus_tag="VJC18_05750"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HLD44920.1"
                     /translation="MGGKSFFHNKLFRSIMFVFLVLLFSTIALYPKTAQATSLAWNTF
                     LGGNSQHLGYGVTTDSSGNVYAVGLTNVNWNTGMFGAPKDSIGYQGGATDGVVAKLDS
                     TGHVLWYRILGGSINDALVNVKVDASGNVYVIGYAFGAYTFTPQATPSPVHGYVGGDI
                     DAFVAKLASNGNLLWHTYLGSTSNDYPWGLSVDADGNVYVGGESYRVWHADFNNVIGT
                     NKSSDGYQGYLVKLNSSGTYQWHRFLTTYAVYEVDASLSGYVHTVKKGLYYSGGWNYP
                     AEVGKYNTTTGEQIWNTTVGSSTQDSFGIDVDTSGNVFISGSSTATWGTPIRAHAGST
                     DIYAAKLNSSGTLQWNTFLGSAGTDVGRSNQPGGISVDAGGNVYTMGYSSATWGTPIT
                     AHAGGSSDGFAVMLNPSGVLVWNTFLGGTNMDTMVALSASAGGDVFVSGYTQQNSWGT
                     PVNFPTGCYYCQSVAKISNSSTLTAIKAGAGAGSVSSAPTGISCGDDCTENFSAGTSI
                     TLTATPDEGSSFAGWSGGTCSGTDPCTFSMGDSVGITATF"
     gene            complement(1789..3132)
                     /locus_tag="VJC18_05755"
     CDS             complement(1789..3132)
                     /locus_tag="VJC18_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44921.1"
                     /translation="MTTLMHTSQLTAALSLLYPASPAFGGLREQRAQSATMISPALLP
                     QHDSAIDSYASVNAGHTSHSQRFNGFRMSPHETTPSRPHIDELCRQYVGRQIRATVTN
                     DSGQNRPVSGRVIDVHVRQGQIFLSIPTRPNIPLSDATFIEDVQPTPVTEPQESNVAE
                     ARKIYGRKWIVITDESGQRIVQVFAVFERDGRVLLQCADVHDAIPVDKVVRLANEAEA
                     KNARQLLEQAIIDRGTSRTKQHAIKASATPPRSVSVKKTFAKKTKPPCAEPPPLPPST
                     EEPAPTVSDLPTSKPMIPSSTHVQGIALPPAFYFDMLAMNVHCSLVHDLTIAPDTGRI
                     TIYASENGLIKSPKNSTLIAYLLDGKLYSPETVEEHEVSFDETNFCPPSRVCANNMIV
                     LVHTNPEGEKQLVGIILKPQHIKPFKTFAQRRQLGTITINKTVFSIDGSGRLSHI"
     gene            complement(3140..4681)
                     /locus_tag="VJC18_05760"
     CDS             complement(3140..4681)
                     /locus_tag="VJC18_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44922.1"
                     /translation="MLQKPVSIVSPPSYSTSGPLDFVDCLPKTWGMIHSVQQALSPRT
                     QEGARYPHPHLSLTNRWRVGRLLTTTSQKTRLHIARRLTDEGLSTAVIHSLLGALEGT
                     PPLTTGQDGHPHYDLDQTVACLSAFGETARDSLMQMLRPSVDATALQKMASSYALLIT
                     AAQHVRDFELPRESGSAVWAILSGLTLIKLAHDAIDPQLLWRSAQNYVTTGINYLPNT
                     STTEAGLRLYIDFAVKELVRIGCQDQLTLTSLFQSERVTAFQRVLLLMTLTRIEKNQE
                     TIDFVTSCYIDSDSPDANSDESRLINQIVFNYLASDTSAFSHSPLLAKFLKDKQNNLD
                     GIPFRYLTAISLLPEDTTQQITSTCASIISSELRYRARKYSIDELLADTAFIKATERG
                     GSAITEIIEKLQQSMFLPSPTHCTNLAIWYLKSHSDNQVCINTVTEYLARAISRNSPE
                     DEIAKILKPLAFQAPDAFAKVFKWAKKHHPKHAIATIIKNLRAEQRRQMASITANVFT
                     HMPVH"
     gene            4961..5614
                     /locus_tag="VJC18_05765"
     CDS             4961..5614
                     /locus_tag="VJC18_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLD44923.1"
                     /translation="MPRINRFDYLANDMLLHVRIQINNGEFKFFEDKHFELFDKICRM
                     YLKKYPTIKLTDYEWMSNHAHFIFHIDVDTDLPKFMHDFCWRFAFEYNKMQNRKGHLF
                     QQRYRASNVNTDQYAMSCQRYVYRNQLRAGMVTDLQDTKWSSYHYYAYGQPNDLITPI
                     FTYELFGNSPEKRQIEFRFFVENVPEHEETLWRAKLLHPLLKTKKEIVLNRMKKCHV"
     gene            5942..>7121
                     /locus_tag="VJC18_05770"
     CDS             5942..>7121
                     /locus_tag="VJC18_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD44924.1"
                     /translation="MSTFFSRFFMTGPIVPVTLKTHEEIDRVYKPKRWSVFLSVTLGY
                     TLFYVCRLNFAIIKKPLMEQGLFNAEQLGLIGSVMFITYAVGKFLNGFFADRLNIKNF
                     MSLGLFATATVSLILGNITYFWFFVVLWGLNGWFQSFGAAPSVVALSQWFSTREMGTY
                     YGIWIISHNLGSGLTAVGTAYFVKWWGWQYGFIVPGAVALIGSYFLYRFMFDRPQTLG
                     LPPVYVYRNDPLQTSQTESVWELQKEVIRKPAVWILGLSSACMYIARYAFESWGIVFM
                     GAQKGYSTVTAGKLVFMSQMFGLVATMLCGYVTDKFFASRRNVLALITGILYAVSLAV
                     FLLTKEKSLFIDCASLAVYGLSLGVLLTFLGGLMAVDICSKRSAGAAMGMIGLFSYVA
                     T"
BASE COUNT         1685 a         1585 c         1869 g         1982 t
ORIGIN      
        1 taaaggtcgc ggtaataccg acgctatctc ccatggagaa ggtgcaaggg tctgttccgg
       61 aacaggtgcc gccgctccac cccgcaaaag atgacccttc gtccggcgtg gccgttaggg
      121 taatcgaagt gccagcggaa aaattttccg tacagtcatc accgcaactg atgcccgtgg
      181 gggcactact gacactaccc gcgcctgctc cagccttgat cgccgtgagt gtggatgaat
      241 ttgaaatctt ggctactgat tggcaataat aacagcccgt aggaaaattg actggggtgc
      301 cccaggaatt ctgttgcgtg tatccagaga caaagacatc accgccggcc gaagcggaca
      361 gggcgaccat ggtatccata ttggtgccgc ccaaaaacgt attccagacc aggacaccgc
      421 ttgggttcaa catgacggca aaaccatccg aagaacctcc cgcgtgtgct gtaatgggtg
      481 ttccccacgt tgccgaactg taacccatcg tgtaaacatt gccaccagcg tccacagaaa
      541 taccgccggg ttgattgcta cggcccacat cggtcccggc tgaccccaaa aacgtgttcc
      601 actggagtgt gccactgcta ttgagtttgg cggcatagat gtcggtgcta cccgcatggg
      661 ccctgatggg ggtgccccaa gtggctgtgc tggatccgct gatgaataca ttgccgctcg
      721 tgtccacgtc tattccaaag ctgtcctgtg tgcttgatcc cacagtggtg ttccagattt
      781 gttcccctgt tgtcgtgttg tatttgccga cctcagcggg gtagttccat cccccagaat
      841 aatacaatcc ctttttgacc gtgtgcacat aaccgctcaa gctggcatcc acctcataga
      901 cggcgtaggt ggtcagaaaa cgatgccatt gatatgtccc actgctgttg agcttgacca
      961 aatatccctg ataaccatcg gatgatttgt tcgttccaat gacgttgttg aaatcggcat
     1021 gccatacacg atatgactca ccgcccacat agacgttgcc atcagcgtct acagaaagtc
     1081 cccagggata atcgttgctg gttgatccaa ggtatgtgtg ccacaaaaga ttgccgttgc
     1141 tggcgagctt ggccacgaag gcatcaatgt cgccacccac atagccatgc acaggcgatg
     1201 gcgtggcctg cggtgtgaag gtgtaggcgc caaaggcgta accgatcacg tagacattgc
     1261 cactggcatc gactttcaca ttcaccagtg cgtcattgat cgaaccaccc agtatacggt
     1321 accaaagcac atggcctgtg ctgtcgagtt tggctaccac accatcggta gcccctccct
     1381 gataaccaat gctatccttg ggggctccaa acatgcctgt attccagtta acattggtga
     1441 gtcctacagc gtacacattg ccactggaat ccgttgtgac cccgtaaccc aggtgttggc
     1501 tgttgccgcc cagaaatgtg ttccaggcca gcgaagtggc ctgtgcggtt ttgggataga
     1561 gcgcgatggt ggaaaacagg agcaccagaa acacgaacat gatggatcga aaaagtttgt
     1621 tatggaaaaa tgatttcccc cccatgcaag cccccacgtg aagttattat tgaggacttt
     1681 tgccagtcac gatgcgtgtc taatattgta acataaaagt gatgtgttgg tgagaaaaaa
     1741 atgtgttttt tacaggtccc ctaacacttg ggaatcataa atataatatc atatgtgtga
     1801 gagacgacca gagccatcga tggagaaaac agttttattg attgtgatag tgccaagctg
     1861 tcttctttga gcaaaggttt tgaacggctt gatgtgttgt ggtttcagaa tgataccgac
     1921 cagctgtttt tctccttcgg gattggtatg gacgagcaca atcatgttgt tggcacaaac
     1981 cctactgggt gggcaaaaat tggtttcgtc aaaggaaact tcgtgctctt cgactgtttc
     2041 tggcgaatac aattttccgt caagcagata ggcgatgagg gtgctgtttt taggagattt
     2101 tataagaccg ttttctgatg cgtagatggt gattctgcct gtgtcaggag caatggtcaa
     2161 gtcgtgaacg agtgaacagt gcacgttcat ggcgagcatg tcaaaataga aagcgggtgg
     2221 aagggcaatt ccctgcacgt gtgtcgatga ggggatcatg ggtttgctgg tcggtagatc
     2281 agaaactgtc ggagcgggct cttctgttga aggcggcagt ggtggtggct cagcgcatgg
     2341 tggttttgtc tttttggcga aggttttttt tacagatact gatcggggtg gagtggccga
     2401 tgccttgatt gcatgttgtt tagttcgtga cgtaccccta tcgattatgg cttgttccag
     2461 taattgtctt gcgtttttcg cctcagcctc gttggccaga cggacaactt tgtcgacggg
     2521 aatggcgtca tggacgtcag cacattgaag taaaacgcga ccatctcttt caaatacagc
     2581 aaacacctga acgatacgct ggcccgattc atccgtgatc acgatccact tacgtccata
     2641 tatctttcgt gcctcggcaa cgtttgattc ttgtggttca gtgacaggag ttggttggac
     2701 atcttcgatg aaggtcgcat cgctaagggg gatgttaggt ctggttggaa tggatagaaa
     2761 gatctgacct tggcgcacgt gaacgtcaat gacgcgcccg ctgactggtc tattctgtcc
     2821 actgtcattg gtgacggttg ccctgatctg gcggccaaca tattgtctgc agagttcgtc
     2881 aatgtgtggt ctggagggtg ttgtttcatg gggagacatg cgaaacccat tgaatctttg
     2941 tgagtgactg gtgtgtccgg cgttgacact agcatatgag tcgattgctg agtcgtgctg
     3001 gggcaaaagg gcaggggaaa tcattgttgc gctttgcgct cgttgttcgc gcaggcctcc
     3061 aaacgcagga gatgcgggat agaggaggct gagagccgca gtcagttgag atgtgtgcat
     3121 cagtgttgtc atatcaattt caatgtaccg gcatgtgtgt aaatacattg gcggtgatcg
     3181 aagccatttg tctgcgttgt tcagcgcgca aattcttgat gatggtggcg atggcgtgct
     3241 tgggatggtg tttttttgcc cacttgaaca ccttggcgaa cgcgtcgggg gcctggaacg
     3301 ccagaggttt taagatcttt gcgatttcat cttcgggtga gtttctagag attgcccgag
     3361 cgaggtactc tgttactgtg ttaatgcaca cctgattgtc actgtgagat tttaaatacc
     3421 agatggcgag attggtacaa tgtgtgggac tgggaaggaa catgctttgt tggagttttt
     3481 cgatgatctc tgtgatcgcg ctcccgccac gttctgttgc cttgatgaac gcagtatcgg
     3541 ccagcagttc atcaatagaa tatttacgtg ccctgtaccg gagttcactg gagatgatgc
     3601 tggcacaagt ggaagttatt tgttgtgttg tgtcctctgg caataatgaa atggccgtca
     3661 aatacctgaa cgggatgccg tcgagattgt tctgtttgtc ctttagaaat ttggcaagca
     3721 aaggactgtg tgaaaatgcc gaggtgtcgg atgccagata attaaaaacg atttggttga
     3781 tgagcctgga ttcatcgcta ttcgcgtcgg gactatcgga atcgatatag cacgaggtga
     3841 cgaaatcaat tgtttcttga ttcttttcga tgcgtgtcag cgtcatcagg aggagcactc
     3901 tttgaaaggc tgtgaccctt tcggattgaa acaagctggt gagtgtgagt tgatcctgac
     3961 agccgatcct caccagctct ttgacagcaa agtcaatgta taagcgcaga ccagcctcag
     4021 tggtggatgt gtttggcaaa tagttgatgc cggtggtcac atagttttgc gcggaacgcc
     4081 agagtagctg cgggtcaatg gcatcgtgag cgagtttgat cagggtcagt ccgctcaaaa
     4141 ttgcccagac tgctgagccg gattcacgag gtagttcgaa atcacgaacg tgttgtgcgg
     4201 ccgtgatcaa caatgcgtaa gaggaggcca ttttttgcaa ggcggttgcg tctacgcttg
     4261 gccggagcat ttgcataaga gagtctctgg ctgtttcgcc aaaggctgac aaacacgcaa
     4321 cggtttggtc caagtcataa tgagggtgac catcttgacc tgtcgttaag ggaggggttc
     4381 cttccagcgc gccaaggaga ctgtgtataa cggctgtgga aagcccctca tctgttaatc
     4441 gacgggcaat gtgcagtctt gtcttttgtg atgtcgtggt cagcagacgt cccacacgcc
     4501 aacgattggt caaagaaaga tgtggatgtg ggtacctggc accttcttgg gtacggggac
     4561 tcagggcctg ttgcacagaa tgtatcatgc cccacgtttt ggggaggcaa tcaacaaaat
     4621 cgaggggtcc agaggtggaa tacgatgggg gagaaacaat tgaaacaggt ttttgtaaca
     4681 tattgattcc ttattgcttg tctgttctaa cctattgaat tattgtttct atcgcacgta
     4741 atttgtaaaa tgttgcgtgt tttttgagtc tgcaaagcgt ttatttacta atttactggt
     4801 gccccatacc ttgtcccaca tatcactgtt agcgtctcat tttcagtccc aacactgact
     4861 caatggtacc acataccata gaatctttca tatcttgttg ttatttcagg catgtttttt
     4921 tttgcagttc tggcactgtc cttgcttgtg actctgtatt atgcccagaa tcaatcgttt
     4981 tgactatttg gccaacgaca tgctgttaca tgtcaggatt cagatcaata atggtgagtt
     5041 caaattcttt gaagacaaac actttgagct attcgataaa atctgtagga tgtacttaaa
     5101 aaagtatccc accatcaaac tcacagatta cgaatggatg agtaatcacg cacactttat
     5161 ttttcatatc gatgttgata ccgatctccc caaattcatg catgatttct gttggcgttt
     5221 tgcctttgaa tataacaaga tgcaaaatcg aaaaggtcat ttatttcaac aacgttatcg
     5281 agccagtaat gttaataccg accaatatgc catgagttgc cagcgttatg tttatcgcaa
     5341 tcagttgagg gcaggtatgg tcacagacct ccaagacaca aaatggagca gttatcacta
     5401 ttatgcatac ggacaaccaa atgacttgat cacccccatc ttcacctacg agttatttgg
     5461 caacagccca gaaaaaagac agattgagtt caggtttttt gttgagaacg tgccagaaca
     5521 cgaagagact ctgtggcgtg ctaaactttt acacccgctt cttaaaacaa aaaaagaaat
     5581 tgtattgaac cgcatgaaaa aatgtcatgt ttgagcatga tcaatacagt gaacacatca
     5641 aatattttca caaacaaatt accttacgtc gattcatgta aaaaatcatg ttacaacatc
     5701 atgtcctcat gatgaccatt gttggttagt cactgaaatt agtcgataga ttatcaaatc
     5761 atctgcaatt tgagtaaagt atgtggcacc tctgcttttg agtaaagtat gtggcacctc
     5821 tgcttaagta tgtggcacct ctgcttttct taagtatgtg gcacatctgc ttctactgac
     5881 aggtttgtaa tttgttcgac agccttcagt cttgttagta ggtatgcttg gggaggctga
     5941 tatgtccact tttttttcca gattctttat gacgggtccg attgttcctg tgacgctgaa
     6001 aacacacgag gaaatcgatc gtgtctacaa gccgaaacgc tggtcggttt tcttgtctgt
     6061 cacactgggg tacacgctgt tctatgtttg ccggttgaac tttgcgatca tcaaaaaacc
     6121 gctgatggaa caaggcctct tcaatgcgga acagttgggt ctcattggat ccgtaatgtt
     6181 catcacctac gcggttggaa agtttctgaa cggctttttc gcggatagac tcaacataaa
     6241 aaacttcatg tctttgggac tttttgccac agccacggtc agcctgattc tgggcaacat
     6301 cacgtatttc tggttcttcg ttgttctgtg gggtctgaac ggctggttcc agtccttcgg
     6361 tgccgcgcca agcgtggttg cgctgtcgca gtggttttct acgcgcgaga tggggacgta
     6421 ctacggtatc tggatcatca gtcacaatct ggggtcggga ctcacggcag tcggtacggc
     6481 ctattttgta aagtggtggg gttggcaata cggattcatt gtgccgggtg ctgtggcact
     6541 cattggctcc tattttttat acaggttcat gtttgaccgt ccacaaacgc ttggcttgcc
     6601 acctgtgtat gtgtaccgga acgatcccct gcagaccagc cagacagaaa gtgtttggga
     6661 attgcagaaa gaggtgatca gaaaaccggc tgtgtggatt ctgggtttgt ccagcgcttg
     6721 catgtacatc gcccgctacg cctttgagag ctgggggatc gtgttcatgg gcgcgcaaaa
     6781 aggatattcc actgtgacgg cgggcaaact tgtcttcatg agccagatgt ttggactggt
     6841 ggccaccatg ctttgtgggt acgttacaga caaattcttt gccagcagac gcaatgtgtt
     6901 ggccctgatc acggggattc tatacgcggt ctctttggct gtgttcttgc tcacgaagga
     6961 gaagagcttg tttatcgact gcgcctcact ggctgtgtat gggctgtcgt tgggagtcct
     7021 gctcacattt ctgggaggcc tcatggcagt ggacatctgt tccaaacgtt ctgcgggagc
     7081 ggcgatgggg atgatcgggc ttttcagcta cgttgccacg g
//
LOCUS       DATDUN010000416         1736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_145458, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000416 DATDUN010000000
VERSION     DATDUN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145458"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..510)
                     /locus_tag="VJC18_05775"
     CDS             complement(49..510)
                     /locus_tag="VJC18_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HLD44925.1"
                     /translation="MNKTTRQQLLLSVIKKEVITSQEELLLGLQQKGAKTTQATLSRD
                     ICELGIIKKGGRYQCSQVEEALSFLGQVQALEPVTGPLLIMKTTAGLANGVAEWIDQQ
                     KFSEVIGTIAGDNTILIALNASAPPQNFIKKISHLFSKTNSRSRRSRSSSI"
     gene            complement(775..1365)
                     /locus_tag="VJC18_05780"
     CDS             complement(775..1365)
                     /locus_tag="VJC18_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HLD44926.1"
                     /translation="MSIVNPQTKEINCKIVYYGPALSGKTTSLLMLQEQFKNKKKKGV
                     VHQVPKTERTMFFDFMALSSKEIIRGYRSKFQVYTVPGQVLYEDSRKLLLHGVDGIIF
                     VADSRLEYIEDSLSSLNELALNLSKLGYDPDQIPMVIQYNKRDCPTAGRIDELKKLLN
                     PKGFPDFETVAHKGVGVMESFETILKNVMLDLKKAA"
     gene            1438..>1736
                     /locus_tag="VJC18_05785"
     CDS             1438..>1736
                     /locus_tag="VJC18_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HLD44927.1"
                     /translation="MVIKTDMPTTDLFDPHRDPSRIHLADGAMGTCLINKGFDPASCL
                     VNLNLTHARLIQQTHREYVNAGAEIIYTHTFGANPVFLKRFGLENLLGEINKRAV"
BASE COUNT          457 a          395 c          394 g          490 t
ORIGIN      
        1 attttgattg aaataaactt gccgttcgcc aactgccgat agtttcggtc agattgagga
       61 cgaacggcta cgacgtgaac gagaatttgt tttggaaaaa agatgtgaga tttttttgat
      121 aaaattttgt ggtggtgccg atgcgttcag ggcaatgagg atagtattgt ctcctgcaat
      181 ggtcccaata acttcagaaa acttctgctg atcaatccat tcggccaccc cattggccag
      241 gcccgctgta gttttcatga tcagcaaggg accggtgaca ggttcaagag cttgcacctg
      301 ccccaaaaac gacaacgcct cttctacctg tgaacactga taccgcccac cttttttgat
      361 aatacccaat tcgcagatgt cccgcgataa cgtggcttgt gtggttttag cccccttctg
      421 ctgaagccca agaagcagtt cttcctgcga ggtgatgacc tcttttttga tgacagaaag
      481 aaggagttgc tgcctggttg tcttgttcat tgttcaacac cattccccat gaatgatatg
      541 atgtgttgta tatatgcgta tatatacagt gcgtcaagga aattgttttg aaggttgtgt
      601 gtcagacatt gatggaacga aatttacaat aaatgtttaa atcagtcaaa atgagtgaac
      661 atctcccgca agaggatcta cgctgaagaa ttgggaaaca aattttattt gaccgaaggc
      721 atgtggcacc ttctatactg agcgacaaaa atatttgccg attccagggt ttcctcatgc
      781 cgcttttttg agatcgagca tgacgttttt aagaatggtt tcgaaactct ccatgacccc
      841 cacacctttg tgggctacgg tctcaaaatc gggaaaccct ttgggattaa gcagtttctt
      901 gagttcatcg attcgtccag ccgtggggca atcacgtttg ttgtactgga tgaccatggg
      961 gatttgatcc ggatcgtaac ccagtttgct caaattcagg gccagttcat tgagggaact
     1021 caaggaatct tcgatgtatt ccaaacggga atcggccaca aagataatgc catccacacc
     1081 atgcagaaga agtttgcgcg aatcttcgta taaaacctgc cccggcacag tatacacctg
     1141 aaacttggag cggtatccac gaataatctc cttggacgaa agggccatga aatcaaaaaa
     1201 catcgtacgt tccgtcttgg gaacctgatg caccacccct tttttctttt tgtttttaaa
     1261 ctgttcctgg agcatcagaa ggcttgtcgt cttgcccgac aaagccgggc cgtaataaac
     1321 tattttgcaa ttaatttctt tggtttgtgg attgactata gacatgcgcg gcaaattatg
     1381 tcgcgggtta ggggatgtca ataacctttt tccttgtttt gaggccaaac aatttctatg
     1441 gtaatcaaaa ccgatatgcc cacaacagat ttatttgatc cgcatcgcga tccctccaga
     1501 atccacttgg ccgatggtgc catggggacg tgtctgatca acaaaggctt tgaccctgcc
     1561 tcctgtctgg tcaacctcaa tctgactcac gccagactta ttcagcagac ccacagggaa
     1621 tacgttaacg cgggagcgga aattatttac acgcatacct tcggcgccaa tcctgttttt
     1681 ctgaaacgat ttgggctgga aaatctgctg ggcgagatca acaaacgcgc tgttga
//
LOCUS       DATDUN010000417         3470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_187115, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000417 DATDUN010000000
VERSION     DATDUN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187115"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1862
                     /locus_tag="VJC18_05790"
     CDS             48..1862
                     /locus_tag="VJC18_05790"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HLD44928.1"
                     /translation="MTQDQNQPSPQDNTTTDKPCCPCHSKWVWIPTLVILVLLAFAAF
                     AYYYYFHRPVPSLTTDKTFINLSEIAAPVNTKVFFAFQPDTQGKLYALSKKTYQNFQK
                     NPATKKFLDEINKSYGIIWDPKLFNTLDPQGMSLMLWPLQEDQSLLSAVKTTSPDGSA
                     PFGLAALLNLGPKASVAAIISQALSSFQNQEEFTAGKGQIADHDVSFLQSKAHPGLKL
                     QWSFWNELLVFATNDRDMTTVLNRLDKGTNDLAQAPLYQKTKTTLAQNTETLFFANLE
                     NMFKQTKQDPKAPLSPHDQFLNALQYLNFSMGTQGNVGTFVSNLVWDPTRFGPIGEAL
                     TTIDQTLTPQYSKLASQSATYTFSLNARALWQMVTKIVALYPALSAQKSSVLAGIFAK
                     GVDIDKDILANITGELSLIIELVQAKPEAPQNADPAAMINNVMKMFEHSYVMIGTKNS
                     AALKDVLQKLMGPTPTELADEPYQNYTLTNLGQFAIASSAENVLVAMSPDALKGIINQ
                     AQAGNVLEKKGLYNTLSGSIDNKTAFLQMYLNFPAYADYMAGMLTSSVPTDKPEMAKL
                     VDDNVKALQMVKELYGDTLTSYSIEKDGIKMLGAMELK"
     gene            complement(1909..3078)
                     /locus_tag="VJC18_05795"
     CDS             complement(1909..3078)
                     /locus_tag="VJC18_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLD44929.1"
                     /translation="MKRKNTGNYIVSTVAGETVRAFVPDPLPPHPFVVWSNDLIQKQE
                     EAAIALGRLDSMTSFLPEPSLFLYSYVRKEAVLSSQIEGTQSSLSDLLAFENAGAPGL
                     PVDSDVVEVSNYVAAMEHGLKRLRGGFPLCLRLLCEIHAILLSKGRGHAKQPGSFRTT
                     QNWIGGTRPGNATYVPPPHHVLSDCLGALEKFLHDDPVKTSVLEKAALIHVQFETIHP
                     FLDGNGRVGRLLIALLLHWQGALNQPLLYLSLYFKKNRQVYYDLLQSVRSDGDWEAWL
                     SFFFEGVRETANQAVATAKCLLSLYEKDKQRIASQGRVAASVLQVHHYFQGAPLSSPN
                     IIVKTLKLSSATVNKALDYLQETQILREVTGRQRDRLFSYHRYLKILNEGTEGAS"
     gene            complement(3287..>3470)
                     /locus_tag="VJC18_05800"
     CDS             complement(3287..>3470)
                     /locus_tag="VJC18_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44930.1"
                     /translation="TGVYHPDRLYRFPFPAHAGMNRLIVIAGTDIYSVPRTRGDEPIT
                     GFFEVGQLCRAPHMRS"
BASE COUNT          964 a          944 c          754 g          808 t
ORIGIN      
        1 tttaattgtt tgttgcgctc ataccgacca aacccgaaag gatttttatg acccaagatc
       61 aaaaccaacc ctcaccgcag gataacacaa cgaccgataa accctgttgc ccctgtcaca
      121 gcaaatgggt ctggatacca actttagtca ttctcgtgct cctggccttc gcggccttcg
      181 cctactacta ttatttccac cggcctgtcc cgtcattgac gacagataag acttttatca
      241 acctttctga aatagcggcc cccgtcaaca ccaaggtttt ttttgccttt caacccgaca
      301 cacaaggcaa attgtacgcg ctcagcaaaa aaacctacca gaattttcaa aaaaacccag
      361 ctacaaaaaa atttctggat gaaatcaaca aatcttacgg aatcatttgg gatcccaagc
      421 tcttcaacac cctcgatccg caaggcatgt ctttgatgtt atggcctctc caagaagatc
      481 aatcccttct ttcggccgtc aaaacaactt cccctgatgg ctcagccccc tttggcctgg
      541 ccgctctgtt gaacctgggt cccaaggcca gcgtggccgc catcatcagt caggccttgt
      601 cgtcctttca gaatcaggaa gaattcacag ccggcaaggg gcaaattgcc gaccatgatg
      661 tgtccttcct gcaatccaag gcccatcccg gactcaagct gcagtggtcc ttttggaacg
      721 aactgctcgt tttcgccacg aatgatcgtg acatgaccac ggtactgaat cggctcgaca
      781 agggaacgaa cgatctggcc caggccccct tgtaccaaaa gaccaaaacc actctggccc
      841 aaaatactga gaccctcttc tttgccaatc tggaaaacat gttcaaacaa acaaaacagg
      901 atcccaaagc ccccctctcc ccacatgatc aatttctcaa tgcgctacag tatttgaatt
      961 tttcgatggg cacacaagga aatgtgggca catttgtctc gaatctggtc tgggatccca
     1021 cccgctttgg tccgattggt gaagccctca ccacgatcga ccagaccctg actccacaat
     1081 attcaaaact ggcctcgcaa tcagccacct acactttcag tctcaacgca cgcgcgctct
     1141 ggcagatggt cacaaaaatt gttgctttgt atccagccct gtctgcccaa aagagcagtg
     1201 ttttggcggg catcttcgcc aaaggcgtcg atatcgacaa agacattttg gccaacatca
     1261 ccggtgaatt gtccctgatc attgaactgg tccaggccaa accagaggca cctcaaaacg
     1321 cggaccccgc cgccatgatc aacaatgtca tgaaaatgtt tgagcattcc tatgtcatga
     1381 ttggcacaaa aaacagcgcg gcgctcaaag acgtcctgca aaaactcatg ggtcccactc
     1441 ccacagagct ggcagatgaa ccctaccaaa attacaccct caccaacctc ggccaatttg
     1501 ccatcgcatc ttcagccgaa aatgtcctcg tagccatgag tcctgacgcg ctgaaaggca
     1561 tcattaacca ggcacaagca ggcaacgtcc tcgaaaaaaa gggtttgtat aacaccttgt
     1621 caggttcaat tgacaacaaa acggcgtttc tgcaaatgta tctcaatttc cccgcttatg
     1681 ccgattacat ggccggcatg ctgaccagta gtgtccccac agacaagccc gaaatggcca
     1741 agctcgttga cgataatgtc aaggccctgc agatggtaaa ggaactgtac ggcgacaccc
     1801 tcacctcgta cagcatcgaa aaggacggga tcaaaatgtt gggggccatg gagttgaaat
     1861 agaaagtaaa cttcgcgtgc ctctctaccg ttggatcgtc ccctcctact aactcgcgcc
     1921 ctctgtccct tcgttcaata ttttcaaata ccgatgatat gagaataacc gatccctctg
     1981 tctgccagtc acctcacgca gaatttgtgt ctcttgcaaa tagtccaagg ctttgttcac
     2041 agtcgcggaa gagagtttca atgttttaac tattatgttg ggcgaactca acggggctcc
     2101 ctgaaaataa tgatgaacct gtaaaacaga agcggctacc cgaccttggc tggcaattcg
     2161 ttgcttgtct ttttcataaa gtgacaagag gcatttggcc gttgccacag cttgatttgc
     2221 tgtttcgcgc acaccctcaa agaaaaagga cagccaagct tcccaatctc catcgctacg
     2281 aacgctctgc aagagatcgt aataaacctg acgatttttt ttaaaataga ggctcaaata
     2341 caaaagtggt tggttaaggg ctccctgcca atgcaagagc aaggcaatga gcaggcgtcc
     2401 cacgcgtcca ttcccatcca gaaaagggtg gatcgtttca aattgaacat gaatcaaagc
     2461 cgctttttca aggacagatg ttttgacagg atcatcatgc aaaaattttt caagggctcc
     2521 gagacaatca gaaagcacgt ggtgaggagg aggaacatag gtggcattgc ccggcctagt
     2581 tccaccgatc cagttttgag tagtccgaaa gctccccggc tgtttcgcat gtcccctgcc
     2641 ctttgacaga aggatcgcat gaatctcgca gagcaacctt aaacagagtg gaaatcctcc
     2701 acgcaaccgt ttcagaccgt gttccatcgc ggcaacatag tttgagactt caacaacatc
     2761 gctgtccacg ggcagtcccg gagccccagc attttcaaac gccagaagat ccgacaaaga
     2821 ggattgagtc ccttcgattt gtgaggagag aactgcctct ttccggacat agctgtataa
     2881 aaataatgat ggctcaggta aaaaagatgt catactatca agacgaccca aggcgatcgc
     2941 cgcttcttcc tgtttttgaa tcaggtcatt agaccagaca acaaacggat ggggtggcaa
     3001 agggtccggg acgaaggcac gaacagtctc acccgctacg gttgacacaa tgtaatttcc
     3061 ggtgtttttt ctcttcataa gactatttta gaaactaaaa taagagctaa cgttatttta
     3121 caatatggca gttttgtaaa ataaagcaac tcttaaaagg aatggtcata atcggtcggt
     3181 aagcgacacc ctcacctcgt acagcatcga aaagagcggg atcaaaatgc tgggggccat
     3241 ggagttgaag tgaggcacgc actgtttttc gctaagttct ctcagttcaa ctccgcatgt
     3301 gcggggcacg gcaaagctgt ccgacctcaa agaatccggt gatcggttca tccccgcgtg
     3361 tgcggggaac ggaataaata tccgttccgg caatcacaat taaccggttc atccccgcgt
     3421 gtgcggggaa cggaaaacga tacaggcgat cggggtgata aacaccggtt
//
LOCUS       DATDUN010000418         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_603829, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000418 DATDUN010000000
VERSION     DATDUN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603829"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VJC18_05805"
     CDS             <1..841
                     /locus_tag="VJC18_05805"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLD44931.1"
                     /translation="GSKKAKIIAVCSQKGGVGKTTTAVNLASALVKFHKKKVLLTDLD
                     PQGHVEKSLATVIEEGIDYSPISQILLAKKGEILDGVVKTTLENLHITPGDKALYETE
                     GQLASKLGREFILHNTFATAQTHYDFIIIDCPPSLGNLTINALCAADYAVIPCEMSVL
                     AFEGVTDLIDTLETIKTRLNRKLHVLGVLFTRVDRRNVMMNEIVEENMKKQFHNRVFK
                     NQITINTALNKAQLEGEPVFDFAPSSSGAKNYAALANELLGKIPKAGGKRVSKANDKR
                     VVA"
     gene            complement(861..1676)
                     /locus_tag="VJC18_05810"
     CDS             complement(861..1676)
                     /locus_tag="VJC18_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HLD44932.1"
                     /translation="MDKLAEQFGINIDYYAVLIAWGLIFTRTFTMLILTPFLGGRGIP
                     GRVRMMTAIVLATFVFYFMNDDLVNKIPADKGIIIILFFKEIFVGLALGITTIMVFYA
                     IEAGGRIVDTQRGSANAQVFLPALGQVSIFGLFQFWLGLAIFCYFGGHVIFLKAFLEG
                     FITIPIFSLPHVAAGISPFLELFIRMSAEVLIFGMQIAAPVLIAIFLTDLVLGIANKM
                     APQIPVFEIGFMMKGYVGVVMTWISITVVASQMEVFFGIMQTNVQKVIHYFGV"
     gene            complement(1697..1963)
                     /gene="sctS"
                     /locus_tag="VJC18_05815"
     CDS             complement(1697..1963)
                     /gene="sctS"
                     /locus_tag="VJC18_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctS"
                     /protein_id="HLD44933.1"
                     /translation="MDAYMTSIAKQALYLVLIMSAPPIMAALLIGLFVSVLQATTQVQ
                     EQTLTFVPKLVAIILVVALLGPLGAVKLMQFTTQIFQGIPVYIH"
     gene            complement(1983..2753)
                     /gene="sctR"
                     /locus_tag="VJC18_05820"
     CDS             complement(1983..2753)
                     /gene="sctR"
                     /locus_tag="VJC18_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HLD44934.1"
                     /translation="MKKLLVALCALTMSFLFEAVAYAQTSTTIDDQPISKPILILFIL
                     FIMSLAPFILMMTTSFVKIAVVLSLVRSAMGTQQIPPNQIITGLALILTVYVMIPVAQ
                     QVYDASSDVIKTADANQELLSTASVDVLKRAVEKGKEPVRQFLLKHVNDKERKLFYGL
                     GIKLKINASIGGLEDSDFIVLIPAFVISELSAAFQIGFVIFLPFLIIDMVVSNILLSM
                     GMFQVSPITVAMPFKLLLFVLVDGWYLITEGLVRGYIT"
     gene            complement(2750..3058)
                     /locus_tag="VJC18_05825"
     CDS             complement(2750..3058)
                     /locus_tag="VJC18_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HLD44935.1"
                     /translation="MFIAQTTGIETLSYGVLFAKTVVIMVVIIGLAFVSIKYLVPRLV
                     KIRNKKSATIEILDFLALEQRKAIYVVRIEEKKLALAVTDHAVAKLCEWDESAPREAS
                     "
     gene            complement(3076..>3286)
                     /locus_tag="VJC18_05830"
     CDS             complement(3076..>3286)
                     /locus_tag="VJC18_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HLD44936.1"
                     /translation="EIPVQMAAVLAKKTMRLSEILTLNVGEVLEFKKQPNEPLDLVAN
                     GKLVAKAELVVVDGKLGVRIVKLVR"
BASE COUNT          937 a          891 c          763 g          695 t
ORIGIN      
        1 gggatccaaa aaagccaaga tcatcgctgt ctgttctcaa aaaggtggcg tgggcaaaac
       61 caccacggcg gtcaacctgg catccgccct ggtaaagttc cacaaaaaaa aggtcctgct
      121 caccgatctc gatccgcagg gacatgtcga aaagtctttg gccacggtga ttgaagaggg
      181 cattgactac agccccatct cacaaatcct gctggccaag aagggggaaa ttctggatgg
      241 cgtggtcaaa acaacactgg aaaatctgca catcacgcca ggtgacaagg ccctgtacga
      301 aacggagggc cagcttgcct ccaaactcgg acgtgaattc attttgcaca acacgtttgc
      361 aaccgcacaa acccattacg atttcatcat catcgattgc ccgcccagtc tcggcaactt
      421 gaccatcaat gccttgtgcg ccgcggatta tgcggtgatc ccgtgcgaga tgagcgtgct
      481 ggcctttgag ggcgtcaccg atctcattga cacactcgaa accatcaaaa ccaggctcaa
      541 ccgcaaattg catgttttgg gggttttgtt cacgcgtgtc gatcgcagaa acgtcatgat
      601 gaatgagatt gtcgaagaga acatgaaaaa acagtttcac aaccgtgtgt ttaaaaatca
      661 gatcacgatc aacacggctc tcaacaaggc ccaacttgaa ggtgagcctg tgtttgattt
      721 tgcaccgtcg tcttcgggcg ccaaaaacta cgccgccctc gccaatgaac tgctgggcaa
      781 aattccaaaa gcgggtggta agagggtctc caaggccaat gataaacggg ttgtcgccta
      841 aagggaacct tccataacct tcacacaccg aaatagtgga tcaccttctg tacgttggtt
      901 tgcatgatgc cgaaaaagac ttccatctgc gaggccacca cggtgatcga gatccaggtc
      961 atgacgaccc ccacgtagcc cttcatcata aagccgattt caaacacggg gatctggggc
     1021 gccattttgt tggcaatccc caagaccagg tcagtcagga aaatagcgat cagcacgggc
     1081 gcggcaatct gcatcccgaa gatcaagacc tccgccgaca tcctgatgaa gagctcgaga
     1141 aaaggagata tcccggcggc cacatgcgga agggaaaaaa tggggatcgt gatgaacccc
     1201 tccaaaaagg cctttaggaa aataacatgc ccaccaaaat aacaaaagat ggccaaaccc
     1261 agccagaatt ggaacaaacc aaagatcgac acctggccca aggccggtaa aaacacctgc
     1321 gcgttggccg acccacgttg cgtgtcgacg atcctgcccc ccgcttcaat cgcgtaaaat
     1381 accatgatcg tggtgattcc caaagccaac ccgacaaaga tttccttgaa aaacaggatg
     1441 atgatgatcc ccttgtccgc gggaatttta ttcaccagat cgtcattcat aaaatagaac
     1501 acgaaggtcg caagcacgat ggctgtcatc atgcgcaccc tgccgggaat accacgacca
     1561 cccagaaacg gtgtaaggat aagcatcgtg aacgtgcgcg tgaagatcag cccccaggca
     1621 atcagaacag cgtaataatc gatgttgatg ccaaattgtt ccgccagttt atccacaaag
     1681 acccttttgg acacctttaa tgtatataaa ccggtatccc ctgaaaaatc tgcgtcgtga
     1741 actgcatgag cttcaccgcc cccaaaggcc ccagcaacgc aacaaccaaa atgatggcaa
     1801 ccagtttggg aacaaatgtg agggtctgct cctgcacctg ggtggttgct tgcagaacgc
     1861 tcacaaacaa tccaatcaac agcgcggcca tgatgggcgg agcagacatg atgagaacca
     1921 gatacaacgc ctgtttggcg attgaggtca tgtaagcatc catagtgttg ctctccctat
     1981 actcaggtga tgtagcctct caccaacccc tcagtgatca ggtaccagcc gtccaccaaa
     2041 acaaaaagca ggagtttgaa aggcatggcc acggtaatgg gcgacacctg aaacatcccc
     2101 atcgacaaca aaatattgga cacaaccatg tcaatgatga ggaacggcag aaatatcaca
     2161 aaccctatct ggaacgccgc tgacaattcg ctgatcacga aggcagggat caggacgatg
     2221 aaatcggaat cttccaaccc cccgatcgaa gcgttgattt tcagtttaat gcccagacca
     2281 taaaacaatt tccgttcttt atcgttgacg tgcttgagca aaaactggcg aacaggttcc
     2341 ttccctttct ccaccgcacg tttcaacaca tccaccgatg ccgtagacaa caactcctga
     2401 ttcgcatcgg ctgttttgat cacatcgctc gatgcatcgt acacctgttg agcgacaggg
     2461 atcatcacgt acacggtcag gatcaaggcc aaaccggtga taatctggtt gggcgggatc
     2521 tgctgtgttc ccatcgctga cctgaccaag gacagcacca cagcaatttt gacaaacgaa
     2581 gtggtcatca tgaggatgaa aggcgccagc gacatgatga acagaatgaa taaaataaga
     2641 atgggcttcg aaatgggttg gtcatcaatc gtggtactcg tctgggcata agcgacggct
     2701 tcgaatagaa aggacatggt caacgcgcac agcgcaacaa gcagtttctt caagaagcct
     2761 cccttggggc tgactcgtcc cattcacaaa gcttggccac ggcgtgatct gtgacagcca
     2821 aagccagttt cttttcttcg atcctgacca cataaatggc tttacgctgt tccagggcca
     2881 gaaaatcgag aatttcgatc gtggcagatt ttttgtttcg gattttcacc aagcggggaa
     2941 ccaggtactt gatactgacg aacgccaaac cgataatcac aaccatgatc acaacggtct
     3001 tggcaaacaa cacaccataa gaaagggtct cgatgccggt agtttgagcg atgaacatgc
     3061 agattgtcct tcaggtcacc taaccagttt gacgattctc actcccagtt taccatccac
     3121 cacaaccaat tccgccttgg ccaccaattt gccattcgcc accagatcca gcggttcatt
     3181 gggttgtttt ttgaattcca gcacttctcc gacattcagt gtgaggatct cgctcaaacg
     3241 catggtcttc tttgccaaca cagccgccat ctgcacggga atctcg
//
LOCUS       DATDUN010000419         1648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_62072, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000419 DATDUN010000000
VERSION     DATDUN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62072"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1426
                     /locus_tag="VJC18_05835"
     CDS             272..1426
                     /locus_tag="VJC18_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44937.1"
                     /translation="MYKREISVPKNNSYFLFGPRGTGKSTLIKQLYPDRESLYVDLLL
                     FQTENQYARDPDLLYRQVKALSDEKKVVIIDEIQKIPKLLDVVHKLIEETDKRFILTG
                     SSARKLKQGGVNLLAGRAFERTLHPLTSAELGKDFDLLQALQWGTLPGLGRQVNASDR
                     MDFLQSYANTYLKEEIVSEQLIRKLDPFRKFLEVAAQHNGIIINFSNIAEDVGVDSKT
                     VKSYFSILEDTLVGFVLEPYLQSTRKRVHQSPKFYFFDIGVCRALANLLTVVPAMSTA
                     YYGTLFEHFVILEFIRREAYARKGFRFYYLSDERQEVDLIIERPGKALALIEIKSAIH
                     IGERQVKKLRNFKSSFPSAEFYCLSQDPTTQSFDGIMAYHWQDGLNHLTQ"
     gene            complement(1456..>1648)
                     /locus_tag="VJC18_05840"
     CDS             complement(1456..>1648)
                     /locus_tag="VJC18_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLD44938.1"
                     /translation="TIGVWCSYVALKHTSAGIAATIIATLPIMVLPVVMIVYKEKITW
                     RALVGTLLAVCGVALLFLR"
BASE COUNT          475 a          370 c          363 g          440 t
ORIGIN      
        1 tcgttaatca ccgcgcgtgt gtaggctgtg cgcggcacgg cggtcgatga catcggttgc
       61 gaaaaaaaat cacagtaccc gcatttgctg acacagaaag ggaaatggat gtagatatga
      121 gcgggcatgg ataacctatt caccaagtgc gtcgtatgtt gtcttgaagg cacttatggc
      181 tgactccata aattttttgc atatttttct tttctttttt caaaaaatgg atcatactcc
      241 gtattatcgt cgaaaacaag gagtgtggtc tgtgtacaaa agagagattt cagttccaaa
      301 aaacaacagt tattttctgt ttggtcccag aggaacgggc aaaagcacgt taatcaaaca
      361 attgtatccc gatcgagaaa gtctctatgt tgatttgttg ctgttccaaa cagaaaatca
      421 gtatgcccga gatcctgact tgttgtaccg tcaagtgaag gcactgtctg atgaaaaaaa
      481 agtggttatc atcgatgaaa ttcaaaaaat ccccaagttg ttggatgtcg ttcataaact
      541 catcgaagaa actgacaagc gattcatact gacaggatca agtgcccgca aactaaaaca
      601 aggtggtgtg aacttgttgg ccgggagggc ttttgaaaga actttgcatc cgctcacctc
      661 ggcagaactg ggcaaggatt ttgatctttt gcaagccctg caatggggaa cccttcctgg
      721 acttggtcgc caggtcaacg cgtctgatcg catggatttt ttacaatctt atgccaacac
      781 ctatttgaaa gaagaaatcg tcagtgaaca attgattcgc aaactcgatc cctttagaaa
      841 atttttggaa gttgccgcac aacacaatgg aatcatcatc aatttttcaa acatagcgga
      901 ggatgtgggc gttgattcca aaacagtgaa atcctacttt tcaattcttg aagacacact
      961 ggtaggtttt gtcctcgaac cttaccttca atcaacacga aaacgtgttc atcaatcacc
     1021 aaaattctat ttttttgaca tcggggtatg ccgtgccttg gcaaatctcc tgactgtagt
     1081 gcctgccatg tcgacggcct attacggcac actcttcgaa cattttgtga ttttggagtt
     1141 catcagacgt gaagcgtacg cgcgcaaggg attccgtttt tattacctgt cagatgaaag
     1201 acaggaagtt gatttgatca ttgagcgacc cggcaaagct ctggctttga tcgaaatcaa
     1261 atcagccatt catattggtg aacgtcaggt aaaaaaactc agaaacttca aatcaagttt
     1321 tccatcggca gaattctact gtttgagcca agatcccacg acccagtctt ttgacggcat
     1381 catggcgtac cactggcaag atggtttgaa ccatttgacc caatgatgaa aatcattggc
     1441 atatcaattc tgccctcacc tcaaaaaaag tagcgccacg ccgcacaccg ccagcaaagt
     1501 ccccaccaac gcccgccatg tgatcttttc cttgtagaca atcatcacca caggaagcac
     1561 catgatggga agcgtggcaa tgatggtggc cgctattccg gcagaagtat gtttgagggc
     1621 aacgtaggag caccataccc cgatcgtg
//
LOCUS       DATDUN010000420         5355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_477129, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000420 DATDUN010000000
VERSION     DATDUN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477129"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VJC18_05845"
     CDS             complement(<1..343)
                     /locus_tag="VJC18_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HLD44939.1"
                     /translation="MKITKNLLPALVLAVFIFGCNCHSCGDGGGNTTPTTTNLVSATD
                     PADGADGVFINRKIAATFSGEMDSATITDTTFTIMQGTTSVSGAVAYSGVTAVFTPAS
                     NLVVNTEYTATI"
     gene            complement(368..1513)
                     /locus_tag="VJC18_05850"
     CDS             complement(368..1513)
                     /locus_tag="VJC18_05850"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HLD44940.1"
                     /translation="MKYKILNTLVMALFVAFFLWGCLSGSGGNSTGTLSVGLTDASTD
                     QYQAVYVTVSEIQVHQSADGEETEEGWISVGTPNATYNLLDLVNGVRESVGLSDLDTG
                     HYTQMRLIVGENPDSTINLLSQPHPFANYVIDLEGDLHELKVPSGMQSGIKIVQGFEI
                     NVNETTELVLDFNASASVVVAGNSGQYLLKPTIQMLDTHEAAIISGDVTQSSEVLTGA
                     TVSAQIFDDMAVDAEDRVTVQAATLSDDAGHYSLFLSPGTYNLVFYKEGFQAFVAKIT
                     VVAGDSVTQDATLDAATTGTLLLTSNISDTDSEAHATTSFRRNTTVDDVNVEIELFVL
                     NIADGGDGLITLPTGDVVVVTSSFEETTQSITVVIDADTQTVLNVSL"
     gene            complement(1613..1768)
                     /locus_tag="VJC18_05855"
     CDS             complement(1613..1768)
                     /locus_tag="VJC18_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HLD44941.1"
                     /translation="MLGTILIVFLVLMVVGVLPTWPHSRNFGYYPSGIGGTLLIIVVI
                     LLLMGRI"
     gene            complement(1826..2185)
                     /locus_tag="VJC18_05860"
     CDS             complement(1826..2185)
                     /locus_tag="VJC18_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44942.1"
                     /translation="MKKFSKMTMMACATGALFLSLGLMGIGCGAEEAINDFNSKNTCE
                     DYCAKNFECNSVAPTTDETDVCVSDCRASMENDCGNENQDAANDQINECVDKGCAEFW
                     ACMVFDAAPECYGFVSE"
     gene            complement(2277..3269)
                     /locus_tag="VJC18_05865"
     CDS             complement(2277..3269)
                     /locus_tag="VJC18_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLD44943.1"
                     /translation="MKNKSYQIIAFIIFLSWLSGCASLGIAVLYKKVNLPENQVIRNL
                     NYSLDEGSKNAMTRLDLFLPKGTNWPTMVFVHGGGWNTGDKDLNVAGADVYGNIGRYF
                     ASQGIGTAIISYRLMPDVDWKTQVMDVARATAWVYEHIPEYQGDQKSIFLAGHSAGAQ
                     LASRVALDRSALLSLGLSPQILCGVIAVSGAGYNFMSHEMYEYGKEQGAIEAIFNRTE
                     LSKILRKKLSPIFFAKQAAPPFLILYAARDEKEIKHDSLRFDQALKNVGAQRQLYSVP
                     KTNHKTMILALSHANKFPASLMTVFMKTCECGTSTTRIFNSSPTPPEKVRKSPS"
     gene            complement(3311..3487)
                     /locus_tag="VJC18_05870"
     CDS             complement(3311..3487)
                     /locus_tag="VJC18_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HLD44944.1"
                     /translation="MLRWSISFFLVAVLAAVFGFTGIAADAVSIAKVLFVVFLVLFIV
                     SLIVPRWRRNQGIN"
     gene            complement(3501..3707)
                     /locus_tag="VJC18_05875"
     CDS             complement(3501..3707)
                     /locus_tag="VJC18_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44945.1"
                     /translation="MQNWLPIILLLIVFAGVACDNGFEKAGANVVRELQKTGDKIDQG
                     LKTTGEDIQKNLDKAGDKVEDVTK"
     gene            complement(3820..4497)
                     /locus_tag="VJC18_05880"
     CDS             complement(3820..4497)
                     /locus_tag="VJC18_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLD44946.1"
                     /translation="MKKPLNISSIKKTKIFIVDDHPILRQGLTLLINNEEDMIVVGEA
                     ENAPEAIKGIEKERPDLAIVDISLEGTSGLELTKNILVKQPKLLVLIISMYDESVYVE
                     RVVRAGAKGYVNKREVSEHVISAIRKVLDGKIYVSDQWKDKLVHRFSNKGTATTAPFV
                     DGLSDRELEVLQMTGQGQTTRQIAEELHVSVKTVESHYANIKNKLDLKNSHELIQFAV
                     KWCLSEK"
     gene            4747..>5355
                     /locus_tag="VJC18_05885"
     CDS             4747..>5355
                     /locus_tag="VJC18_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HLD44947.1"
                     /translation="MVPDLKQIKFVYMGTPDFAQVILKHLHQSGANIVAVFAQPDRPS
                     GRGQQLTCPPVALWAKANGLSVFQPENLKNDFTVNHIAALNPDFIVAAAYGNLLPERI
                     LRTPKQESLNVHASLLPHYRGAAPINYALLNGDAQTGVSIMRMVKQLDAGPVFATQAL
                     PLLHEDNAITLTHKLARLGGELLITTIGGILMGSCKPTEQNDT"
BASE COUNT         1447 a         1333 c         1149 g         1426 t
ORIGIN      
        1 tgatcgttgc cgtatattca gtattcacaa ccagattgct ggccggtgta aaaacggcag
       61 taacgccaga gtaggccaca gcacctgaaa cagacgttgt tccctgcatg atggtaaaag
      121 tggtgtctgt gatcgttgct gaatccatct ccccgctgaa ggtcgcggct atttttctgt
      181 tgatgaatac gccatcagca ccatcagccg gatcagtcgc actcacgagg ttggttgtgg
      241 tgggtgttgt gttgcctcct ccgtcgccac agctatgaca attgcatcca aaaataaaaa
      301 cggccagaac caaagccggc aacaaatttt tcgtgatttt cataattttc ccttcaaaaa
      361 acgtgtctta cagactcaca ttcaatacgg tttgcgtgtc ggcatcgatc acaactgtga
      421 tcgattgagt tgtttcctca aaagaggagg taacgacaac cacatcgccc gtgggcaaag
      481 tgatgaggcc atcaccgccg tcggcaatgt tgagaacaaa cagttcaatc tcaacattga
      541 catcatcaac agtggtgttt cgacgaaaac ttgtggtcgc gtgagcttct gaatcggtat
      601 cgctgatgtt ggaggtcagc aaaagcgttc ctgtcgtggc agcatcaagg gtggcgtcct
      661 gcgtaacgct gtcacccgcg acaacggtaa tttttgcaac gaacgcttga aaaccttctt
      721 tataaaaaac caggttgtac gtgccgggtg atagaaaaag tgaatagtgg ccagcatcat
      781 ctgaaagagt ggccgcctga accgtcacgc gatcttcggc gtccacagcc atgtcatcaa
      841 atatctgggc tgaaacagtt gcgcctgtca gcacctctga tgattgtgtc acatccccgc
      901 tgatgatggc ggcttcgtgt gtatccaaca tctgaattgt gggcttgagt aaatattggc
      961 cgctattccc ggcaaccaca actgaagcgc tggcattaaa gtcaagcacc agctctgttg
     1021 tttcatttac attaatctca aaaccctgca caattttgat ccctgattgc attccactgg
     1081 gaacttttaa ctcatgtagg tctccctcca agtcgatcac ataattggca aagggatgag
     1141 gctggctcaa aagattaatg gtgctgtcgg gattttcgcc aacaatcaag cgcatctgcg
     1201 tgtaatgccc tgtatcaaga tcagagagcc ccacggactc gcgcacacca ttgaccaggt
     1261 cgagcagatt gtaagttgcg tttggcgttc caaccgatat ccaaccttct tcagtttctt
     1321 ctccatcggc agattgatgg acttgaattt cgctcacggt cacatacacg gcttgatact
     1381 gatccgtgga ggcatcggtg agtccaacag acagagttcc tgtgctattt ccaccactcc
     1441 cgcttaaaca accccacaga aagaacgcaa cgaatagtgc cattacgaga gtattcaata
     1501 ttttatattt catagaaatt acctttttga aattttcata ttcccaacat tcaaacccaa
     1561 atcgacacct cagccctggc aacaccagag atgagagaca gaagaccgat ttttaaatgc
     1621 gtcccatcag aaggagaata acaacgatga tgagcaaggt gccgccaatt ccgctggggt
     1681 agtagccaaa attgcggcta tgcggccaag tcggtaaaac gcccaccacc atgaggacca
     1741 ggaagacaat aagaattgtt cctaacatat tatgttctcc tttttatttc ttgagatatt
     1801 ttaacaaaat cgttcatttt cttttctatt cactcacaaa gccgtaacac tctggcgcgg
     1861 catcaaagac catgcaggcc cagaattcgg cacatccttt atcgacacac tcattaatct
     1921 gatcattggc agcgtcctgg ttttcgttac cgcaatcgtt ttccatggaa gcgcggcagt
     1981 cgctcacaca gacatctgtt tcgtcggttg ttggggctac gctgttacat tcaaaatttt
     2041 tggcacagta gtcttcgcag gtattttttg aattgaaatc gttgatagct tcctccgcac
     2101 cgcagcctat gcccatgagg cccaaagaca gaaacagggc acctgtcgcg cacgccatca
     2161 ttgtcatctt ggaaaatttt ttcatgttaa aactcctttg aattgttttt ggtttcatca
     2221 cctgtaggag aaatacaaaa gaatggtaaa agaaaaaatc aggggagaca gggttttcag
     2281 ctgggggatt tccgcacttt ttcgggtggg gtcggggagg aattgaatat tctcgtcgtt
     2341 gacgttccgc attcacacgt cttcataaag accgtcatga ggctcgctgg aaatttgttg
     2401 gcgtggctca atgccagaat catggttttg tgatttgttt tggggaccga ataaagttgc
     2461 ctttgtgccc ccacattttt cagggcttga tcgaatctta atgaatcgtg cttgatttct
     2521 ttttcatccc tggcggcgta gaggatcaga aaaggtggtg ccgcttgttt ggcaaaaaat
     2581 atgggagaca atttctttct taatatttta ctcagttctg ttctgttgaa aatggcctcg
     2641 atggcgcctt gttcttttcc atattcatac atttcgtgac tcataaagtt ataacctgcc
     2701 ccactgacag caatgacgcc gcacaagatt tgaggggaca accccaaaga cagtaaagca
     2761 gaacgatcaa gagccaccct gcttgccagc tgagcaccag cagaatgccc agccagaaag
     2821 attgatttct gatcaccctg atattcggga atgtgttcat aaacccaggc ggtagctctt
     2881 gctacatcca tcacctgagt tttccaatcg acatcgggca ttaaccgata actgatgata
     2941 gctgtgccaa ttccttgcga agcaaaatat ctgccgatat tgccataaac atctgctcca
     3001 gcaacgttaa gatctttgtc gcccgtattc cagcccccac cgtgaacaaa aaccatggtg
     3061 ggccaatttg tgcccttggg caaaaaaagg tcaagccgcg tcatggcgtt ctttgaaccc
     3121 tcatccaaag aataattgag gtttcgaatc acttggttct cgggcaaatt cactttctta
     3181 tacagaacag caatgcccaa agaggcgcaa ccgcttaacc aggatagaaa gataataaac
     3241 gcgatgatct ggtaggattt atttttcata aacgtattcg aagggaccaa tcatctcaca
     3301 agcgaaatcg ttaattgatc ccctgattcc tccgccaacg cggcacgatc aatgaaacaa
     3361 taaacaagac aagaaatacg acaaacaaaa ccttggctat tgaaaccgca tctgcagcaa
     3421 tccctgtgaa cccaaacacg gcggccaata cggcaaccag aaaaaatgaa atggaccatc
     3481 gtaacatagt gtgctcctat ttattttgtg acatcttcta ccttatcgcc agccttatcg
     3541 aggttttttt gaatatcctc gcctgttgtt ttcaaacctt gatcaatctt gtcgcctgtt
     3601 ttttgaagtt cgcgaaccac gttcgcacca gccttctcaa aaccattgtc gcaagccacg
     3661 ccggcaaata cgatcaataa cagaatgatg gggagccaat tttgcatata agttcttttt
     3721 tgatttattt gtttttaatt tttgaacacc cagagattct aaaggagctg gctcactatc
     3781 acaatcgggg gtgacaggat tttgggatgg gggattttct tatttttctg acagacacca
     3841 cttgactgcg aattggataa gttcgtgaga atttttgaga tcgagtttgt ttttgatgtt
     3901 ggcgtaatga gattcaacag tcttgacgct tacgtgaagt tcttccgcaa tttgacgcgt
     3961 ggtctgtccc tgcccggtca tctgcaacac ttcaagctca cgatcactga ggccatcaac
     4021 aaaaggagcc gttgtagccg tgcctttgtt cgaaaaccga tgcacaagtt tgtctttcca
     4081 ttgatcactc acataaattt taccatccaa caccttgcga atggctgaaa tgacatgctc
     4141 actcacctct cgcttattga catacccctt tgctccagct cgtacaacgc gttccacgta
     4201 aacagactca tcatacatcg aaatgatcag gacaagcaac ttgggttgtt tgactaaaat
     4261 atttttggtg agctcgagac cacttgtgcc ttccaaggaa atatccacga tcgccaagtc
     4321 aggtctttct ttctcaatgc ccttgatggc ctctggcgca ttttctgctt ccccgacaac
     4381 gatcatatcc tcttcattgt tgatcaaaag cgtcagtcct tgacgcagga tcggatgatc
     4441 atcaacgatg aagatcttag tttttttgat gctgcttata ttaagaggtt ttttcaaaca
     4501 agactccttt ggatgagcgg tccttatata tctaaagtat tccaaattca atttacaaac
     4561 gtaacaaaga aagaacaatc agggggaacc atattcttga gcaggggatt gtcctagtaa
     4621 actgtcctaa agcctttttc gtaacagaaa atcacagccc ccctctagga gtcccaacga
     4681 cgaggttctg tcccaaaaag agatacgctt tacaattcaa ccgaatcacg ttactttcca
     4741 aacccgatgg ttccagacct caaacaaatt aaattcgtct atatgggaac ccccgatttc
     4801 gcgcaggtga ttttgaagca tctccaccaa tcaggcgcca acatcgtggc cgtatttgca
     4861 cagcccgaca gaccttctgg ccgtgggcaa caactcacat gccctcccgt tgctctgtgg
     4921 gcaaaagcaa atggcctcag tgtgtttcag ccggaaaacc tcaaaaacga tttcaccgtg
     4981 aaccacatcg ccgccctcaa ccctgatttc attgtggcgg ccgcctatgg aaatctcttg
     5041 ccagaaagaa tcctgcgaac cccgaaacag gagagcctga acgtacacgc atcgcttctg
     5101 ccccactacc gtggcgccgc gcccatcaac tacgccctgc tcaatggtga tgcacagacg
     5161 ggtgtgtcga tcatgcgcat ggtaaaacag cttgatgccg gtcccgtttt tgccacacag
     5221 gcactacccc tattacatga ggacaatgcc atcacgttga cccacaagct ggccaggctg
     5281 ggcggtgaac tgctgatcac aacgatcggc ggcatcctga tgggttcatg caaaccaaca
     5341 gagcagaatg acacg
//
LOCUS       DATDUN010000421         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_354403, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000421 DATDUN010000000
VERSION     DATDUN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354403"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..>2521)
                     /gene="rpoB"
                     /locus_tag="VJC18_05890"
     CDS             complement(112..>2521)
                     /gene="rpoB"
                     /locus_tag="VJC18_05890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLD44948.1"
                     /translation="VCPVETPEGPNIGLIASLSSYARVNDYGFIETPYRVVTKGLVTE
                     EVRYFTALDEEKHVIAQANAKLSKEGKFEDEFVSCRKDGEFALVPRQEVSLMDVSPNQ
                     LVSIAASMIPFLEHDDANRALMGSNMQRQAVPLLTSESPIVGTGMERVVARDSGVATA
                     AGRDGTILSVDASRIVIRADRVKKKDSGSEIDIYNLIKYQRSNQNTCINQVPLVQAGM
                     KVKLGDIIADGPSTEKGELALGKNLVVAFMPWGGYNFEDSILLSEKLVRDDVLTSIHI
                     EEFECVARDTKLGKEEITRDIPNVGEEALKDLDETGLVRIGAEVKSGDILVGKVTPKG
                     ETQLSPEEKLLRAIFGEKAGDVKDTSLRVGPGVVGTVIATQIFSREGVELDDRAKDQQ
                     SQATKKLKKDQIDEIKAIQISYEKQLRQLFVGQTASSDALDESGKVAIKEGKKIAAEV
                     LTKLPISYWKNISINDEEVEEQIEVLFDDMEDQIDMVAMVYDQKVNKLRKGDELPPGV
                     IKLVKVYVAIRRKIMVGDKMAGRHGNKGVVSIVLPEEDMPYLEDGTPVDIILNPLGVP
                     SRMNIGQVLETHLGWAGLKLGQKIEAMIERSFDRDDAIKFLKKVFHDKDQQQIFLNMT
                     DKELIDFCRNQKEGIRFATNVFDGATENEIKDLLEMADIDRSGQHIVFDGRTGEAFAN
                     PVTVGVLYMLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAY
                     GAAYTLQEFLTVKSDDVVGRTRMYERIVKGDHTLEPGLPESFKVLMKELQGLSLNIEL
                     VEEK"
BASE COUNT          585 a          730 c          545 g          661 t
ORIGIN      
        1 tcgtggatcg tggatcgtgg atcgtacttg gacacaataa ccaatatgcc cctcaacaga
       61 tcgcaaatag catctgcgac caacgattca cgattcacga tccacgattt ttcacttttc
      121 ttccaccaat tcaatattca aactgaggcc ctgcaattcc ttcatgagca ctttaaatga
      181 ttcgggcaaa cctggttcca atgtatggtc tcccttaaca atgcgttcgt acatgcgggt
      241 ccggcccacc acgtcatctg acttgaccgt cagaaattct tgcaatgtgt aggcggcacc
      301 ataggcctcc aaggcccaaa cttccatttc tcccagtcgt tgccccccaa attgggcctt
      361 accacctaat ggttgctgcg tgaccaacga ataaggcccg atagacctcg cgtggatctt
      421 gtcatcaacc aagtgatgca acttcaacat atacaatacg cccacagtca cggggttggc
      481 aaaggcctcc ccggtgcggc catcaaacac aatgtgttgt cctgaacgat caatgtccgc
      541 catttcgagc agatctttga tttcattctc cgttgcccca tcaaaaacat ttgtggcaaa
      601 ccgaatgcct tccttctgat tgcggcaaaa atcgatcagt tccttgtccg tcatgttcag
      661 aaagatctgc tgttggtctt tgtcgtgaaa cactttcttg agaaacttga tggcgtcatc
      721 gcgatcaaag gagcgttcaa tcatggcctc aatcttctgc cccaatttga gccctgccca
      781 acccaagtgt gtctccaaaa cctgtccaat gttcatacga ctgggcacgc ccaagggatt
      841 caagatgatg tctacgggcg ttccatcctc gagataaggc atgtcttctt cgggcagcac
      901 gatgctcacg acccccttgt ttccgtggcg gccggccatt ttgtcaccca ccatgatttt
      961 gcggcgaatc gccacataaa ctttgaccag cttgatcacg ccaggaggca gttcgtcccc
     1021 cttgcgaagt ttgttcactt tttgatcgta caccatggcc accatgtcga tctggtcttc
     1081 catgtcatca aataacacct cgatctgctc ttcgacttct tcatcgttaa tggaaatatt
     1141 cttccaatac gatatgggca gtttcgtcaa cacctcggcc gcgattttct tgccttcctt
     1201 gatcgccacc ttgccactct catccagggc atcggaagaa gctgtctggc caacgaacaa
     1261 ttgacgcaat tgtttttcgt aactgatctg aatggccttg atctcatcga tctggtcttt
     1321 tttgagtttt ttggtggcct gactctgttg gtccttggca cgatcatcca actcaacgcc
     1381 ttcacgtgag aaaatctgtg tcgcgatcac agtacccacc acacccggac caaccctgag
     1441 tgacgtgtct ttgacatcgc cggccttttc accaaaaata gctcgcagga gtttttcttc
     1501 tggtgacagc tgggtttcgc ccttgggtgt gactttaccc accaggatat cccccgactt
     1561 cacttcggcg ccaatacgca ccaaccctgt ttcatcgaga tcctttaagg cctcttcgcc
     1621 aacgttggga atgtcacgcg tgatttcttc cttgcccaac ttggtgtcgc gggcaacaca
     1681 ctcaaattct tcgatgtgaa ttgacgtgag gacatcgtca cgcaccagtt tctccgacaa
     1741 caggattgag tcttcaaaat tataaccgcc ccagggcatg aacgccacca ccagattttt
     1801 cccaagagcc aattcgccct tttctgtcga aggaccatca gcaatgatgt cgcccagttt
     1861 caccttcatg cctgcctgca caagaggcac ttggttgata caggtgtttt gattcgaacg
     1921 ctgatattta atgaggttgt aaatgtcgat ttcggaaccc gagtcttttt tcttcacgcg
     1981 gtcggccctg atcacgatgc gcgaggcatc aaccgacaag atcgttccat cccgacctgc
     2041 cgcggtggcc acacctgaat ctctcgccac cacacgctcc ataccagtcc ccacgatagg
     2101 ggattcactg gtcagcaagg gaactgcctg acgttgcatg ttggacccca tgagggcgcg
     2161 gttggcgtca tcgtgctcca aaaacggaat catggaggca gcgatactga ccaactgatt
     2221 gggagacacg tccatcaaag acacttcctg acgcggaacc aacgcgaact ccccatcttt
     2281 gcgacacgac acaaactcat cctcgaattt gccttctttt gacagtttgg cgttggcctg
     2341 agcgatgacg tgtttttcct catccaaggc cgtgaaatag cgcacttcct cagtcaccag
     2401 ccctttggtg accacacggt aaggcgtctc gatgaatccg taatcattga cacgagcata
     2461 agatgataat gacgcaatca aaccgatgtt tggaccttcg ggtgtttcga cgggacacac
     2521 g
//
LOCUS       DATDUN010000422         1519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_227207, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000422 DATDUN010000000
VERSION     DATDUN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227207"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /gene="rplB"
                     /locus_tag="VJC18_05895"
     CDS             complement(<1..351)
                     /gene="rplB"
                     /locus_tag="VJC18_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583348.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HLD44949.1"
                     /translation="MRSFRPTSAGVRFKTVPDYSEITRGYPEKSLTETLVRSAGRNAH
                     GHITSRFRGGGHKRKYRVIDFRRNKVDVPAKVFSIEYDPNRSSYIALLVYADGEKRYI
                     IAPEGLKVGSTVMAS"
     gene            complement(448..732)
                     /locus_tag="VJC18_05900"
     CDS             complement(448..732)
                     /locus_tag="VJC18_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HLD44950.1"
                     /translation="MNLYDVIKQPVVTEKSSFLKESGDVYVFEVDKKATKIDIKKSIE
                     KLFSVKVKAVKTLVQRGRAKRFGKGHGQTKSWKKAVVTLKEGKIEVFEGV"
     gene            complement(729..1346)
                     /gene="rplD"
                     /locus_tag="VJC18_05905"
     CDS             complement(729..1346)
                     /gene="rplD"
                     /locus_tag="VJC18_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HLD44951.1"
                     /translation="MELQVIDKNNKKVSTIAVSDEMGLKPSQDVLYYAVKAARNNLHH
                     GTACVKDRSDINKTNKKIYRQKGTGNARHASRRANIFVGGASAFGPHPRSFGEHVNKK
                     FKKRSYQEVFKYLIQGDKIRVLNEINFDKPSTKAAKTFLSKIGLTKAVVFLSAENENA
                     KKSFRNLKDVSVMNENNLSLYEILRHDAVVMTEVYFTQVKERYGL"
BASE COUNT          359 a          402 c          297 g          461 t
ORIGIN      
        1 cgatgccatg accgtactgc cgaccttgag gccttctggt gcgatgatgt atctcttttc
       61 cccgtcggca tacacgagca atgcgatgta tgaagatctg ttgggatcgt actcaatcga
      121 aaacaccttg gcgggcacat caactttgtt gcgtctgaaa tcgatcacac ggtacttgcg
      181 cttgtgacca ccaccgcgaa aacggctcgt gatgtggcca tgagcattac gtcccgcact
      241 cctgaccagg gtttctgtca atgatttttc gggataccca cgagtgattt cactgtaatc
      301 gggcaccgtt ttaaatctca caccagcact tgttggtcta aaacttctca ctttgttact
      361 ccctcatttt caacacaatt cacaacccgc aaacaacacc cgtttcataa aacggtcgcc
      421 gccttacgga tggcaaagtg ccattgatta cacaccttca aaaacttcga ttttaccctc
      481 tttcaaggtc accacagctt ttttccagct ttttgtttga ccatgacctt tgccaaagcg
      541 tttcgctcga ccacgctgga ccagtgtctt caccgctttg accttcacag aaaacagctt
      601 ttcaatactt tttttgatat caatcttggt ggcctttttg tccacttcga acacatagac
      661 atcaccagac tctttcaaaa aactgctttt ttccgtaaca acaggttgtt taatgacatc
      721 gtataagttc ataagccata tctttctttg acttgtgtga aatacacctc agtcatcacc
      781 acggcgtcgt gccgcaggat ttcgtacaaa ctaaggttgt tctcattcat cacactgacg
      841 tccttcaggt ttctgaaaga cttctttgcg ttttcatttt cggccgacaa aaacacaaca
      901 gccttcgtca aaccaatctt gctcaaaaac gtcttggccg ctttcgtcga tggcttgtcg
      961 aaattgattt cgttcaacac cctgatcttg tcgccctgaa tcaggtattt aaaaacttcc
     1021 tgatagcttc tctttttgaa tttcttattc acatgctcgc caaacgatcg ggggtgcggc
     1081 ccaaaggcgg aagccccccc cacgaagata ttcgcgcgac gtgacgcatg acgggcattc
     1141 cctgtcccct tttgacgata gattttttta ttggtcttat tgatgtcgct tctgtctttc
     1201 acacaggcag tgccatgatg caaattgttc cgggccgctt tgacagcgta gtacaagaca
     1261 tcctgactgg gcttgagccc catctcgtca ctgacagcaa tcgtggagac ttttttattg
     1321 tttttatcga taacctgtaa ttccataaaa tacctctaac cgatttggtg ttttttatga
     1381 ctgaggaccc tcttgcggtt tgtttgccgt aaacaccctg ttctcaaaat cctgcgcgtg
     1441 tgtgtaaaga taaaccaaag cgtttctgga accgggcaga gcgcctttga tcaacacaat
     1501 gttctgctca ggttcggtg
//
LOCUS       DATDUN010000423         2315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_270143, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000423 DATDUN010000000
VERSION     DATDUN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270143"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1350
                     /locus_tag="VJC18_05910"
     CDS             100..1350
                     /locus_tag="VJC18_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD44952.1"
                     /translation="MAQKYWSIEGVFLKSAIDLRIFNTPFGSISLLYGPRQIGKTASL
                     RLFLSKLTDSETIIFTDCSAILNKADLTQHLAELIQGPTTIVLDEVQEVEGWHLALRS
                     LHAIGKLQQCRIWCTGSEARHLLESGERLPGRKGQGISVFARPWSFREFMDFLHPDQT
                     QSLRKLKHNHITQQWLNEQNFLWQNYWQHYLMTGGIPQVVGEFYTTGKISDATWNIYV
                     DWILGTWSKQRTAERSLTALARQLCETLNSRVSFETLKKGTDILSPNTIKTLIEMQED
                     HFSLKLLPRFDPQKNRFLPIKLKKIYPIDPFIARVWASIGWNVRRLMSQNVPTPHLDE
                     CAFLTQTYRWQNGPETSYLYSDTKKAELDFYFEGIAFELKSGGKPTASQRSLLNQCPQ
                     SFFLNHKTLPLMAYLLGEGRELDT"
     gene            1427..>2315
                     /locus_tag="VJC18_05915"
     CDS             1427..>2315
                     /locus_tag="VJC18_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44953.1"
                     /translation="MPLLTSNIIPSAPLTQGAPWFSTLSHSITTGLARSLQSIANTGS
                     KYLSGTSAVRMGADITPTEFKSVKTPTRTAPDTRLETASTPVVDNPESIPVLPPPTAP
                     PQQSPSSFDPVEVYKSFQGTIDLMLRFVAQWAAAPFMYSSGKELVGSVYFRGEQPETF
                     GGVCGNPFKEMTPPADPMDWRYSGAFDRGVAYLFWIPANFVSICLDTYLLRELERRHQ
                     GGQITSADYESGKKIIKSCLLANKFMLADTALFVGSFLYMSHPEHSEAVGLPLLVATQ
                     VGNVVAHAAMLRRNDLVIRH"
BASE COUNT          638 a          607 c          507 g          563 t
ORIGIN      
        1 tcatcatgca atatattatc attaattcaa atgcagatat atattaacca agaattattt
       61 aatgataaca atataatact taaaagcccc gcactcgaca tggcgcaaaa atactggtcg
      121 atagaaggtg tttttttaaa atccgcaata gatttgcgga tttttaacac tccgtttggc
      181 tccatttcgc ttttgtatgg ccctcgtcaa attggcaaaa ccgcctctct cagattattt
      241 ttgagcaaac tgactgattc tgaaaccatc atttttacgg attgttctgc catactcaac
      301 aaagctgacc tcacccaaca tttggccgag ctcatccagg ggccgacaac catcgtcttg
      361 gatgaagttc aggaggttga aggctggcat ttggcgctca ggtcgctaca cgccattggc
      421 aaactccagc aatgccgtat ctggtgcact ggcagtgaag caaggcactt gctggaaagt
      481 ggtgaaagat taccggggcg caaaggccaa ggcatctctg tctttgctcg tccctggagc
      541 ttcagggaat tcatggattt tctgcacccc gatcaaacgc agtcgctcag aaaactcaaa
      601 cacaatcaca tcacgcaaca gtggcttaac gaacaaaatt ttttatggca aaattattgg
      661 caacattatc tgatgaccgg tggcatccct caagtcgttg gggaatttta cacaacgggc
      721 aagatttctg acgctacctg gaacatctat gttgattgga ttctgggaac ctggtcaaag
      781 caacgaacgg cagagcgaag cttgacagcc ttggcccgac aactttgcga aacactcaac
      841 tcacgagtca gctttgaaac gctcaaaaaa gggactgaca tcctgagccc caacacgatc
      901 aaaaccttga tcgaaatgca ggaagatcat ttttccctga aacttcttcc cagatttgat
      961 ccccaaaaaa atcgattttt gccgatcaaa ttgaaaaaga tttaccccat tgatcccttc
     1021 atcgccaggg tctgggccag catcggctgg aatgtaagac gcctcatgtc gcaaaacgtc
     1081 cccacccccc accttgatga atgtgctttt ttgacacaaa cgtatcgttg gcaaaacggt
     1141 cccgaaacca gttatttata cagtgacaca aaaaaagcag aactggattt ctattttgag
     1201 ggaatagcat ttgaactaaa atcaggaggc aaaccaacag caagccaacg atcgctatta
     1261 aaccagtgtc cgcagagttt ttttctgaat cacaagacat tgcctctcat ggcttacctt
     1321 ttgggagaag gccgcgagct tgatacataa aagacaaact gtttaccggc tttgcgatga
     1381 gcatcaacgt tgacaacccg tctcacaacg aataaacgcg cacgcgatgc cattgctcac
     1441 ctcaaacatc ataccatctg cgcccctcac tcagggtgcg ccctggttca gcaccttatc
     1501 ccacagcatc accactggct tggcgcgttc tttacaatcc attgccaata ccggatcaaa
     1561 atatttatcg ggcacaagcg ctgtcaggat gggtgcggac atcacgccca cagaattcaa
     1621 atccgtcaaa acaccaacga ggacagcacc agacacacgg cttgagacag catccacacc
     1681 ggtcgttgac aatccagaat cgatacctgt tctccctccc ccaaccgctc ccccacaaca
     1741 atcgccatct tcatttgacc cggtagaggt ctacaagtct tttcagggca cgatcgattt
     1801 gatgctcaga ttcgtggccc aatgggccgc cgcgccgttc atgtattcca gtggcaagga
     1861 gttggtgggc tcggtgtatt tcaggggaga acagccggaa acctttgggg gtgtgtgcgg
     1921 caaccctttc aaagagatga cacctcccgc tgatcctatg gattggcgat acagcggggc
     1981 ctttgatcgc ggagtggcat acctgttttg gataccagca aattttgtat ccatctgtct
     2041 cgatacctat cttctgcgtg agctggaacg ccgccatcaa ggtggccaaa tcacatccgc
     2101 agattatgag agcggcaaaa aaatcatcaa gtcctgtttg ctggccaaca aattcatgct
     2161 ggccgataca gcgttattcg tgggctcatt cttgtacatg agtcatccgg aacactcaga
     2221 ggcggtcgga ctcccactgc tcgtggccac gcaggtaggc aacgtagtgg cccacgccgc
     2281 catgttgcgc agaaatgatt tggtcatcag gcata
//
LOCUS       DATDUN010000424         1142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_187204, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000424 DATDUN010000000
VERSION     DATDUN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187204"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VJC18_05920"
     CDS             <1..420
                     /locus_tag="VJC18_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44954.1"
                     /translation="EEDAIRTNCARLGVKLIPKSLAGFVPICVDRRSTIDAILIDDDP
                     LIHSTWHSVARMKKKKIIAYKNTHDFLSECSRIDKTTPLYIDSKLSDGVKGEVAAKTI
                     HAKGFENIYLATGTPASEFGPMPWIKGIVGKVPPFAK"
     gene            complement(433..777)
                     /locus_tag="VJC18_05925"
     CDS             complement(433..777)
                     /locus_tag="VJC18_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLD44955.1"
                     /translation="MTNDSLTFPFVSVRQLKTILDNKPDDVFLLDVREADEFAFTHIK
                     GFVNIPISQFEQRVLAELDKNKTAFVISHLEGRALAACAYLHHQGYENVTCVQGGLTA
                     WSCQIDAKIRRY"
     gene            complement(782..>1142)
                     /locus_tag="VJC18_05930"
     CDS             complement(782..>1142)
                     /locus_tag="VJC18_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44956.1"
                     /translation="QTTQKQTQRALVSLGYQIMDTDESRNRILTGWVPTTSDSHYLEY
                     FNRRDYGAGAGSYYQLVADVFQEGNQVKVSVCTTIKSISGKLTSSRVVEKRLLAKLDD
                     FMRSPQIVITNVGMQEH"
BASE COUNT          281 a          270 c          300 g          291 t
ORIGIN      
        1 gaggaagatg cgattcgcac gaattgtgct cgattaggcg tgaagttgat acccaaaagt
       61 ctggcggggt ttgtgcctat ttgcgtggat cgacgatcca cgattgatgc catcctcatt
      121 gacgatgatc ccctcattca ctccacgtgg cattctgtgg cgcggatgaa aaagaaaaaa
      181 atcattgctt ataaaaacac ccatgatttt ctgagtgaat gtagcagaat cgacaaaacc
      241 acgccgctgt acatcgattc caagctatca gacggggtga agggtgaggt ggcggccaaa
      301 accattcacg ccaagggatt tgaaaatatc tatctggcca ccggaacgcc tgccagtgag
      361 tttggcccca tgccgtggat caagggcatt gtgggcaaag tcccgccttt tgcaaagtga
      421 aaaaaggtgt ccttaatatc ttcttatctt cgcgtcgatc tgacaagacc aagccgttag
      481 cccaccctga acacaagtca cattttcgta tccttggtga tgcaaatacg cacaggcggc
      541 cagtgccctc ccctcgagat ggctgatgac gaaggctgtc ttgtttttgt cgagttctgc
      601 caagactcgt tgttcaaatt gcgaaatggg gatgttcaca aaacccttga tgtgggtgaa
      661 ggcaaattca tctgcttcac gcacatcgag cagaaaaaca tcatcgggtt tgttatcaag
      721 aatggtcttg agttggcgga cagagacgaa gggaaaggtc aatgagtcat tggtcatttg
      781 gtcaatgttc ttgcatgccc acattcgtga tgacgatctg ggggctgcgc atgaaatcat
      841 caagctttgc gagcaaccgt ttttccacca cccgcgagga cgtgagtttt ccggaaatgc
      901 ttttgatggt tgtgcacacg ctgaccttga cctgattgcc ttcttgaaac acatccgcga
      961 caagttggta gtaggacccg gcgccagcgc cataatcacg tctgttgaaa tactccagat
     1021 aatgagagtc gctcgtggtg gggacccagc ccgtgaggat gcggttgcgg gattcatcgg
     1081 tatccatgat ctgatatccc aggctgacca gagctctttg cgtttgcttt tgcgtggtct
     1141 gg
//
LOCUS       DATDUN010000425         1985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_603995, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000425 DATDUN010000000
VERSION     DATDUN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603995"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1985)
                     /locus_tag="VJC18_05935"
     CDS             complement(<1..>1985)
                     /locus_tag="VJC18_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44957.1"
                     /translation="NSAYEEKRCDADVSGASVPAFWGHFTSYNLVEKGLLGEQGYDVL
                     GMSIPYSINDDTIPLIWSVGKIRLKFEDLDPGDDKDESVIQVTREKIGDVWSNKVNII
                     NDAIKNYSQDIVSGNQLSTLFDASKMSAKTIQIESFDNCKSYYQNQHPGYFVPFGCDD
                     EHNKNYPFVLELNSVAGQRVYSDMTASGQNSSFLGIIWWGVMETFKKMVGCMDEELVN
                     PMISSQYKYPAWVWAGSTFSTDFEWEDFDFGANIENADLNIFDGGLTVRLPLSLGVDG
                     VPLTKRIGWKGLASSLPVIKQTGQSTVSTYNQANISGHLRRSSKNKGLDDYPLVSATP
                     QQSAFLGLSLNLEEIFNSAAYLIFKKGPMSLLDTFGIEDIEANNNWSVGVDKVILARF
                     DICDLAGLIPSELPPSLFFATIQSSFSESTLHLDVILDKDYPVTLSLNPVEGLDNATE
                     VQLGVTNLQIGVKELMAHKNEAGETEANVYEDPASQPELLRFRLDGVIRARLVYHKTQ
                     RQIHVYIPGYAEQNIHASVPSGHGGATYDDVNVVTDVLNGVVKALFKKMQKDINEDIT
                     YDDQDKATINVADANPTIIISLKGGDSANAGSLSLSNIDDFEIELKMDAAENATCGDD
                     VAYYDGEQPETALPGKAIKPGSQPGMLGSDQPWKQKD"
BASE COUNT          479 a          534 c          461 g          511 t
ORIGIN      
        1 tcatcttttt gcttccacgg ttgatcggag ccgagcattc cgggctgaga acccggtttg
       61 atagcctttc cgggaagggc tgtttccggt tgttcgccat cgtaatacgc cacgtcatca
      121 ccacaggtgg cgttctccgc cgcgtccatc ttcaactcaa tctcaaaatc atcaatattg
      181 gataggctca aagaacccgc gttggctgag tcccctccct ttaacgagat gatgatcgtc
      241 gggttggcat cagccacgtt gatggttgcc ttgtcctgat catcgtaggt gatgtcttcg
      301 ttgatgtcct tttgcatttt tttaaacagg gccttcacca caccattgag aacatcggtc
      361 acgacgttca catcatcata agtggccccg ccatgtcccg atggcacact ggcgtgaata
      421 ttctgctcgg cgtaacccgg gatgtacaca tgaatttgtc gctgggtttt atgatagaca
      481 agcctggccc tgatcacacc atcgagtctg aatcgcagga gttctggctg acttgccgga
      541 tcctcgtaaa cattggcctc ggtctcgcct gcctcgtttt tgtgagccat gagttctttc
      601 acaccaatct gcaaattggt cacacccagt tgcacttcgg tggcgttgtc caaaccctct
      661 acgggattga gcgacagagt gaccgggtaa tccttatcga ggatcacatc gagatgcagc
      721 gtggattcac taaaggatga ttgaatcgtg gcaaaaaaga gtgatggcgg caactctgat
      781 gggatgagtc ctgccaggtc gcaaatatcg aaccgcgcca gaatgacttt gtcaacaccc
      841 acagaccaat tgttattggc ctcgatgtct tcgataccaa acgtatccag aagcgacatc
      901 ggccccttct taaaaatcag ataggcggcg gaattgaaga tttcttcaag gttgagtgag
      961 aggcctaaaa acgcgctctg ctgtggtgtc gctgacacca atggatagtc atccaacccc
     1021 ttgttttttg aagaccgcct caaatggccc gaaatgttgg cctgattgta cgtgctgacc
     1081 gtgctttgac cggtttgttt gatgacgggc agggacgagg ccaggccctt ccacccgatt
     1141 ctcttggtca gaggaacccc atcgacacca agactcagcg gcaatctgac agtcagaccg
     1201 ccatcaaaaa tattcagatc ggcattttcg atgttggcac caaaatcaaa atcctcccat
     1261 tcaaagtctg tggagaacgt ggaccctgcc cacacccaag caggatattt gtactgggac
     1321 gagatcatgg ggttaacgag ttcttcatcc atacacccca ccatcttctt gaacgtctcc
     1381 atcacccccc accagatgat tcccaaaaaa gaggaattct ggccactggc cgtcatgtcg
     1441 gaataaaccc tctgacccgc tacagaattg agttccagca cgaaggggta atttttatta
     1501 tgctcatcgt cacagccaaa cggcacaaaa taacccgggt gttgattttg ataatacgat
     1561 ttgcagttat cgaaagattc gatctgaatg gtcttggcgc tcatcttgga agcatcaaaa
     1621 agcgtgctca gctgattgcc actcacaata tcctgcgaat aattcttgat cgcatcgttg
     1681 atgatgttga ccttgttgct ccagacatcg ccgatttttt cccgagtcac ctgaatgacg
     1741 ctttcgtctt tatcatcacc cggatccaga tcctcgaatt tcagcctgat tttgcccacc
     1801 gaccagatca gcgggattgt gtcatcattg atggaatacg gtatcgacat gcccaacaca
     1861 tcgtacccct gttcgcccaa gagacccttt tcgaccagat tgtacgacgt aaaatgcccc
     1921 cagaaggcgg ggacgctggc gccagacaca tcggcatcgc aacgtttttc ttcatacgcg
     1981 gagtt
//
LOCUS       DATDUN010000426         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_145648, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000426 DATDUN010000000
VERSION     DATDUN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145648"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VJC18_05940"
     CDS             <1..301
                     /locus_tag="VJC18_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HLD44958.1"
                     /translation="KMKLPQRSRITIEYVMLGGINDSLDDARRLVRLLNGLRVKINLL
                     PFNENPQMPYKRPSHEKVRAFQQYLMDKGLVAIERKSRGQDISGACGQLVTNTVI"
     gene            complement(341..574)
                     /locus_tag="VJC18_05945"
     CDS             complement(341..574)
                     /locus_tag="VJC18_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HLD44959.1"
                     /translation="MKLTVQITPNARKSEVIGSDGHVLKLKIAAPPAEGKANKELICF
                     LSKLWKVPKSSLTILKGHTSRIKILDVPDRIMR"
     gene            complement(579..944)
                     /locus_tag="VJC18_05950"
     CDS             complement(579..944)
                     /locus_tag="VJC18_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HLD44960.1"
                     /translation="MSTIGLLFIILAKICHTLLNMYQFVVIVAVIMTWIRPSPTNDFI
                     RTLLVTAFKLTEPVFSFVRRKLPKSFFSFGIDFTPIIVLFAIYAVDILLTSLFMDIGI
                     RLRMGAMPSAPVDALRQTM"
     gene            complement(1163..1855)
                     /locus_tag="VJC18_05955"
     CDS             complement(1163..1855)
                     /locus_tag="VJC18_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HLD44961.1"
                     /translation="MTTSVASNLEHTKVLIDQAAKKAERQASEITLIAVSKKQTVDDI
                     SEAYSFGQCDFGESYAQELVNKAHLLSQPRIKWHFIGHLQRNKAKQVVGLASLIHTVD
                     RLPLAETINKLAEDKGIMQDCLVQVKLAEEESKTGCEPHEVKGLLGDLNRLKNIRIKG
                     LMLIGTWTTDLRTSQKEFIALRELRNALNKHNAYKEPLEFLSMGMSGDFELAIAHGAT
                     HIRVGTSIFGSR"
     gene            complement(1852..2283)
                     /locus_tag="VJC18_05960"
     CDS             complement(1852..2283)
                     /locus_tag="VJC18_05960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLD44962.1"
                     /translation="MDRKYRVQTILTVRFSDTDAMGHVNNANIFTYMEQGRVAYFNKL
                     FPEKKLSDHYDIFPFILANIKADFKSPAYCGESLIVGLGVVHMGNKSLTMEYDIAEEL
                     TERLVATGSSVLVMYDYASAATYPIPDELKARITELEHSQR"
     gene            complement(2356..2565)
                     /locus_tag="VJC18_05965"
     CDS             complement(2356..2565)
                     /locus_tag="VJC18_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HLD44963.1"
                     /translation="MKKEYDFTKGIRGKFHRTIKIQKTLRLDKDVLEFYQNMAMKNGI
                     PYQTLINLVLKKFVAEEASLVLSPV"
     gene            complement(2549..2836)
                     /locus_tag="VJC18_05970"
     CDS             complement(2549..2836)
                     /locus_tag="VJC18_05970"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HLD44964.1"
                     /translation="MLFIWDTHKATANFRKHKVSFEMAQSVFDDPLHLSVLDTKQHHE
                     ERWVTIGKCVTRNTLIVVHTYYDDRGGHETIRIISARLATRKEKIQYEKGI"
     gene            2917..>3011
                     /locus_tag="VJC18_05975"
     CDS             2917..>3011
                     /locus_tag="VJC18_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006418960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HLD44965.1"
                     /translation="MLSQTIARILLDQKAVTLSPDKPYTFASGIK"
BASE COUNT          781 a          696 c          705 g          829 t
ORIGIN      
        1 taaaatgaaa ctcccgcaaa gaagccggat caccatagaa tacgtcatgc tgggtggcat
       61 caatgattct ctggatgatg ccaggagatt ggtgcgcctg ttgaatggtc tgcgggtcaa
      121 aatcaacctc ctgccattta acgaaaaccc acaaatgccc tacaagaggc cctcgcacga
      181 gaaggtcagg gcgtttcagc agtacctcat ggataaaggg ctggtggcca tcgaacgaaa
      241 atcacgggga caggacattt caggggcctg cggccagctt gtgaccaata cagtcattta
      301 acttttgcgc atggcaggac ccgaactgaa tgtacaaaag ttatcgcatt attcgatcag
      361 gaacatccaa tatctttatt cgggacgtgt ggcccttgag aatggtgagg gacgatttgg
      421 ggaccttcca gagcttcgat aaaaaacaaa tcaattcctt gttggccttg ccttctgcgg
      481 ggggcgcagc gatcttgagt ttgagcacgt gcccgtcact gccaatgact tcgcttttac
      541 gggcgttggg ggttatttga acagtgagtt tcaaaatgtc acatcgtctg acggagtgcg
      601 tccacgggtg ccgacggcat tgcccccatt cgcagacgga tgccgatgtc cataaatagg
      661 gacgtgagca gtatatctac ggcgtaaatg gcaaacaata cgatgattgg ggtaaagtcg
      721 attccgaacg aaaaaaaact tttgggcaat ttgcgcctca caaaagaaaa aacaggttcc
      781 gtgagtttaa atgccgtgac cagcaaggtg cggataaaat cattggtggg ggagggtctg
      841 atccaggtca tgatcacggc aacaatcacg acaaactgat acatattgag cagtgtgtga
      901 caaatcttgg caagaataat aaataataaa ccgatggtag acatggggcg ctagctaaaa
      961 gctcaaagct caaaggtcaa aggaaaaatc ttttatcgac gtaatcggtt acttattgga
     1021 gcgtattgat ttttcgggtc ctgctccggg tcgctatttt ttgacgctct tcgcaagcgg
     1081 ggcttgctcc gcaggccgca acaaaataat agctccccgt atcaccccaa aaatcaaaac
     1141 gctccgttta ctgacggatt gctcatcgac tgccaaaaat gctggtgccg accctgatat
     1201 gcgtagctcc gtgcgcgata gccagctcaa aatcaccgct catacccatt gagaggaatt
     1261 ccagaggttc cttgtaggcg ttatgcttat tcagggcatt acgcagttcc ctgagcgcta
     1321 tgaattcctt ttgactagtc cgtagatcag tcgtccaggt tcctataagc atcaacccct
     1381 tgatgcgaat attttttagt ctgttcagat cgcctaacaa gcccttcacc tcatgaggtt
     1441 cgcaacctgt tttggattct tcctcagcca atttgacctg caccagacaa tcctgcatga
     1501 tccctttgtc ctcggccagt ttattaatgg tttcggccaa tggaagacga tccaccgtgt
     1561 ggatgagcga ggcgagcccc accacctgtt tggccttatt gcgctggagg tgtccgataa
     1621 agtgccattt gattctgggt tgactcagca ggtgcgcctt gttcaccagt tcctgggcat
     1681 agctctctcc aaaatcgcat tgcccaaaac tgtaggcctc gctgatgtcg tccacagttt
     1741 gtttttttga aacggcaatc aaggtgatct cgctggcctg gcgttcggct tttttggcgg
     1801 cctggtcgat cagtacttta gtgtgttcga gattggatgc aacagaggtc gtcatctttg
     1861 agaatgctcc aattcagtga ttcttgcctt caactcatca ggaatggggt aggtggctgc
     1921 gcttgcgtaa tcgtacatga ccaacacact ggacccagtg gccaccaatc gttcggtgag
     1981 ttcttcggca atatcgtatt ccatcgtcaa acttttgttg cccatgtgta ccacgcccaa
     2041 tccaacaatg agcgactcac cacaatatgc gggagactta aaatcggcct taatattggc
     2101 cagaatgaat ggaaaaatat cgtagtggtc tgataatttt ttttcgggaa aaagtttatt
     2161 gaagtacgca acacgcccct gttccatgta agtaaagatg ttggcgttgt tcacatgccc
     2221 catggcatcg gtatccgaaa agcggacggt gaggattgtt tgaactcgat attttctgtc
     2281 cataggaaac tttatagagg cattcttctg aacaatcaat tggcaaaaac tccccggagt
     2341 gatgaaataa cgatcttaaa caggtgacaa taccagagaa gcttcctctg ccacaaattt
     2401 cttgagaact agattgatga gcgtttgata ggggatgccg tttttcatgg ccatgttctg
     2461 ataaaattcc aatacatctt tgtctaaccg cagtgttttt tggattttta ttgttctatg
     2521 aaatttgcca cgaatgcctt ttgtaaaatc atattccttt ttcatattgt attttttcct
     2581 ttctggtggc cagccgggca gatatgattc ggatcgtttc gtgaccacca cggtcatcat
     2641 aatacgtatg cacaacgatc agggtgtttc tggtgacaca tttgccaatc gtgacccacc
     2701 tttcctcatg atgttgcttt gtatccagaa cagataagtg caggggatcg tcaaatactg
     2761 actgcgccat ttcaaatgag accttgtgtt ttcggaaatt cgcggtggcc ttatgtgtat
     2821 cccaaataaa caacattgta gttacagtat agcgtattca tattatttga gcaatagtca
     2881 attgattatc ttgattttct tggttattgc ccagccatgc tgtcacaaac cattgccaga
     2941 attctgcttg atcaaaaggc ggtcaccctc agtcccgaca agccctacac atttgcctcg
     3001 ggaatcaaaa g
//
LOCUS       DATDUN010000427         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_394541, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000427 DATDUN010000000
VERSION     DATDUN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394541"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /locus_tag="VJC18_05980"
     CDS             complement(<1..2099)
                     /locus_tag="VJC18_05980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HLD44966.1"
                     /translation="MKFVTMTDEQQAAVSTPGSLAVIASAGTGKTTVLTQRFLYCHLK
                     RDAPLHQLLAFTFTEKASREMMTRILESQEIPYTQAPLLNISTIHSFCQRLLKQHGSC
                     LGLSPDFEVYDEDSHNVWQEMMTSQYVSNALEKGNECVVNFIRCYGYTNLKTTIRQLL
                     RKDLGSLKPEELVCISEEGLGDEHTNSSPQSGIPSAGRNHELMTGFIKALACFQQELL
                     AHKISHQIVSYDDLEILTGRLFDEQRHILQKVQGQYRHILVDEYQDISPRQFALISKL
                     FNPERNELFIVGDPKQSIYGFRGAEVGLFYDTVKLIEDQGGQVIYLTTTFRTPNQLQR
                     YFNRVFPLVLSGKDFQPARTTKIDEEALLYAAPVPADIKVTAARHEHYANKIAGIAHD
                     RIKAGTRPEEIAVLFYTALPIPIYQTALHERGIKTVTRTSTAVFEVPVVLVAWQIMKY
                     LCGDRDRLTQVGLLRNEIFSFSESFIDHIVKSQGTDCFCGQTVDLFSQTHDLAQWHRL
                     ITWLAKWQDLSRVLFVSELFQTIVSDLRENLSVYESYFVQNVARLLVSWQKEGMFYLT
                     QVAKPLRTVLTKDALALSVPAHPEGVQLLTIHGAKGLEFEHVFLVPGRGKNRETPICL
                     QDPGRGFVFKKHDCENEKMLSYQTEVTPAYERADEVWRHKEALELTRLVYVALTRASR
                     CLYFFVEKPSQSLLSRFA"
     gene            complement(2096..>3072)
                     /locus_tag="VJC18_05985"
     CDS             complement(2096..>3072)
                     /locus_tag="VJC18_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HLD44967.1"
                     /translation="PCAPEKQTLASDYYQSKKKNFSLTELEKYAECPYCYYAACHLKL
                     DRPPVDELEPSADVKGSFVHQVLFRLIAENETLYWEGLEYASYREKLVKKLGHVIQEE
                     IDKSEEFKKYHQKMIEFYAYRVYKTISTLLQLESENYKTAKKKTVPRHYEWSFGSDSR
                     KPLVLDTKAGVVQIRGRVDRVDVCQASRQFSVVDYKTGQAPSLTAIKAVKSLQLPAYL
                     IAVQQLLYPDQAPAGAYYYLLKTNEIKGFSMRETVDAGLMHKRSQLGLVEWQDLQDRF
                     VLRLGEIVEGVRAGHFDPNPASAQLCRFCDYKYLCGYRATECEPEDDE"
BASE COUNT          742 a          815 c          758 g          757 t
ORIGIN      
        1 gcaaagcggc tcaacaatga ctgtgacggt ttttctacaa aaaaatacag acaccggctg
       61 gcccgagtca gggccacata caccagacgt gttaattcca acgcctcctt atgtcgccaa
      121 acctcatccg cccgctcgta agcgggagtg acttcggtct ggtacgagag catcttttcg
      181 ttttcgcaat catgtttttt aaacacgaaa cccctgccag ggtcctggag acaaatgggc
      241 gtttcgcggt tttttccccg ccccgggacc agaaagacat gctcaaactc gaggcccttg
      301 gccccgtgaa tggtcaacaa ctgaacccct tcggggtggg cagggactga caaggccagg
      361 gcgtctttgg tcaagaccgt cctcaacggt ttggccactt gagtcaaata aaacatcccc
      421 tctttttgcc aggagacaag cagtctggcc acgttttgaa caaagtagga ttcgtacacc
      481 gacaaatttt cacgcaaatc agaaacgatg gtctggaata attccgacac aaacaggacc
      541 cgcgacaggt cctgccactt cgccagccag gtaatcagac gatgccattg cgccagatca
      601 tgtgtttgag aaaacagatc cacagtttga ccgcaaaaac aatcagtccc ctgtgatttg
      661 acgatatggt cgatgaagct ttcggaaaaa gaaaaaattt cgtttcgaag caatcccacc
      721 tgcgtcaggc ggtcgcggtc gccgcacagg tatttcatga tctgccaggc caccagcacg
      781 acaggcacct cgaagacggc agtactcgtg cgcgtcacgg ttttgatacc tcgctcgtgc
      841 aacgcggttt gataaatggg aatggggagt gccgtgtaaa acaacacagc aatctcctcc
      901 ggtctcgttc cggccttgat ccggtcatgt gcgatgccag caattttgtt ggcgtaatgt
      961 tcatgccgcg cggctgtcac cttgatgtcg gctggcacag gcgccgcgta gagcaaggcc
     1021 tcttcatcga tcttggtggt tctggccggc tgaaaatcct tcccggagag gaccagggga
     1081 aagaccctgt taaaatagcg ttgcagttga ttgggggtcc gaaaggtcgt tgtgagataa
     1141 ataacctggc caccttggtc ttcgatgagc ttcacagtat cgtaaaaaag acccacctca
     1201 gccccgcgaa aaccataaat ggactgcttt ggatcaccca caatgaaaag ttcattgcgc
     1261 tctgggttga ataacttgct gatgagggcg aactgtctcg gtgagatatc ctgatattcg
     1321 tccacaagaa tgtgtctgta ctgtccctgc actttttgca atatatgcct ttgttcatca
     1381 aaaagtctgc ccgtcaaaat ctccaaatca tcgtaggaga caatctgatg actgattttg
     1441 tgtgccaata actcctgctg aaaacacgcc agggccttga taaatcctgt catgagttcg
     1501 tgatttcgtc ccgccgacgg gatcccgctt tgcggtgatg agttcgtgtg ttcgtccccc
     1561 agcccttcct cagaaataca aacaagttcc tcaggcttaa ggctacccaa atcttttctc
     1621 aacaactggc gtatggtggt tttaagattg gtgtatccgt aacaacgaat gaaattcacc
     1681 acgcactcat tccctttttc gagcgcgttg gacacatatt gtgaggtcat catctcttgc
     1741 catacgttat gggagtcctc gtcgtacact tcgaaatcag gcgacaagcc caggcaactg
     1801 ccatgttgtt tgagcaggcg ctgacaaaag gaatggatcg tcgaaatgtt gagcagaggc
     1861 gcctgcgtgt aggggatctc ctggctttcc agaatgcgcg tcatcatctc gcgagacgcc
     1921 ttttcggtaa aggtgaaggc cagtaattga tgcaggggag cgtctctttt gagatgacaa
     1981 tacagaaacc tctgcgtcag gaccgtggtt ttgccggtac cagccgaggc aatgacggcg
     2041 aggctcccgg gtgtcgaaac agcggcctgc tgttcatctg tcatggtcac aaatttcatt
     2101 cgtcatcctc tggttcacat tctgtcgcgc ggtagccaca aaggtatttg tagtcacaaa
     2161 accgacacaa ctgggccgac gcggggttgg gatcgaaatg ccccgcgcga acaccttcca
     2221 cgatttcccc cagacgcaaa acaaatcgat cctgaaggtc ctgccactcc accaagccaa
     2281 gctgtgaccg tttgtgcatc agcccggcat cgacggtctc cctcatgctg aaacccttga
     2341 tctcgtttgt cttgagcaga taataataag cccctgcggg ggcctggtcg ggatacaata
     2401 attgttgcac agcgatcaag taggcgggca gttgcaaact cttgacagcc ttgatggccg
     2461 tcaaactggg ggcctgcccc gttttgtaat ccacaaccga aaactgcctg ctcgcctgac
     2521 acacatcgac acgatcaacg cgtccacgga tctgcaccac acccgctttg gtgtcgagca
     2581 caagtggttt tcgggagtca gacccgaagc tccattcata gtgacggggg actgttttct
     2641 ttttggcagt tttgtaattc tcagattcca actgcaacaa ggtcgagatc gtcttgtaca
     2701 cgcggtacgc gtaaaactcg atcatcttct ggtgatactt tttgaattct tcggatttgt
     2761 caatttcttc ctgaatcacg tgccccaact ttttgaccag tttttcccga tacgaggcat
     2821 attccagccc ttcccaatac aaagtctcat tctccgcgat gagcctgaag aggacctggt
     2881 gcacgaaact gcccttcaca tcggcagacg gttccagttc atccacgggg ggacgatcaa
     2941 gtttgagatg acaggccgca taatagcaat agggacattc ggcatacttt tcgagttcag
     3001 tgagcgaaaa gttttttttc ttcgattggt aataatccga cgccagcgtt tgtttttcgg
     3061 gcgcgcatgg cg
//
LOCUS       DATDUN010000428         2613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_477411, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000428 DATDUN010000000
VERSION     DATDUN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477411"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VJC18_05990"
     CDS             complement(<1..222)
                     /locus_tag="VJC18_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44968.1"
                     /translation="MNSTPAITVRFLDLECDQYVVSLCPRLGQPPKNVDCLTARYQRS
                     DPYWALDGIKLMGHYWFTDPMSYEPKDQGL"
     gene            341..1096
                     /locus_tag="VJC18_05995"
     CDS             341..1096
                     /locus_tag="VJC18_05995"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44969.1"
                     /translation="MNQSVALWSVPRSCSTALLLSVEAGGQFSQVFNEPFGEPFFHGP
                     LRLSTRYQHEPPNTAYTFDGTMATIEAASQMGTVCFKEMANHVREALLASDRGKAFLR
                     NITHHAFIVRHPSIAIVSLARPAHWPDFTLREAGYEEAWRLFEGLQQLTGRSPVCLDG
                     TILQKDPIGLLSAFFHHLGLPFDSTKLAWTPANPRLFGKWAHEWYAQASKSRGIEAQQ
                     THAEDEGIRQQPHVQQAIEYHMPFFNRLAEHFL"
     gene            1326..1640
                     /locus_tag="VJC18_06000"
     CDS             1326..1640
                     /locus_tag="VJC18_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HLD44970.1"
                     /translation="MDNILAIINQNPKNLMLALSKRAKERRLFYGYKRTTLAKKAGVN
                     VASLKRFELTGQISLVSLLKLAFALDCLAEFSNLFPLPEAHSIKELEQRMKPIRRKRG
                     HL"
     gene            1637..1777
                     /locus_tag="VJC18_06005"
     CDS             1637..1777
                     /locus_tag="VJC18_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44971.1"
                     /translation="MKKLHVFLTDAFGQKKLVGTLAEFDRRIVFEYSNAFLEEHIQMR
                     KH"
     gene            1792..2019
                     /locus_tag="VJC18_06010"
     CDS             1792..2019
                     /locus_tag="VJC18_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HLD44972.1"
                     /translation="MCFNIVFHNRDDHVKNFSYVMDDDGRWKLSLAYDLCFSEGLGGE
                     HFMTVMGEGRQIAREHILKLARETGISELLK"
     gene            complement(2011..>2613)
                     /locus_tag="VJC18_06015"
     CDS             complement(2011..>2613)
                     /locus_tag="VJC18_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HLD44973.1"
                     /translation="RRAAREVLKEFGIRFTNQYYDQHTCGMDDISLFHHFFDLAGKTL
                     SRSLCAQLLKSKHTIFMRMVQTSDIRMAGITRLLKTLKMHNVPLAIVSGSLRSEVKAC
                     LLKCELLDFFEFMVCHEDVQKTKPHPEGYLQALTRMKRHHPQITKKDCWVIEDSPTGV
                     SAAKKAGLPVIALTSSTSAQNLSQADAVVPSLSQLQIMLF"
BASE COUNT          710 a          554 c          655 g          694 t
ORIGIN      
        1 cagaccttga tcttttggtt cgtacgacat gggatcagtg aaccaataat gtcccatgag
       61 cttgatgcca tcaagtgccc aatagggatc ggatctttga tagcgagcag tgaggcaatc
      121 aacatttttt gggggttgcc ccaaacgtgg gcagagagag acaacgtact gatcgcattc
      181 gagatccaaa aaacgcactg tgatggctgg agttgagttc acatcgttgc ctgacatcgg
      241 attttaatgc catagccaca gatcgtttgt caaaaataat acacaacttt tgcccagtgc
      301 tcacgatatg tgggctaggc catgacaagg gaaagatgat atgaatcaat cagtagcatt
      361 atggagtgtt cccagatcat gctctacagc attgttgttg tctgttgaag caggcgggca
      421 gttttcgcaa gtgtttaatg aaccatttgg agagcctttt tttcatggtc cactgcgtct
      481 ttccactcgt taccaacacg aacccccaaa tactgcgtat acttttgatg gcaccatggc
      541 aacaattgaa gcggccagcc agatgggcac cgtgtgtttc aaagaaatgg caaatcatgt
      601 gagagaagct ctgttagcct ctgatagagg taaagcattt ctaagaaaca tcactcatca
      661 tgcctttatt gtgaggcatc cttcgatagc gattgtgtcc ttggccagac cagcacactg
      721 gcctgatttt acattacgtg aagcaggtta tgaagaggcg tggcgactat ttgaaggttt
      781 gcaacagtta acaggcaggt ctccggtttg tcttgatggc acgattctgc aaaaagaccc
      841 tattggtctc ctctctgctt tttttcacca cttaggtttg ccatttgaca gcaccaagct
      901 agcttggact cctgccaatc cacggttgtt tggaaaatgg gcccatgagt ggtatgccca
      961 agcgagcaaa agcaggggga tcgaagcaca acaaacacat gctgaagatg aggggataag
     1021 acaacaacct catgtgcaac aagcaataga gtatcacatg ccatttttta acaggttggc
     1081 tgaacatttt ctataaaggt gccacagact tgagcctttg aagaacgcac gtggacagca
     1141 agaggcagag cttgtggcaa aacacttcgc tgtgagagag ctgtgtcagg gctgatggtg
     1201 actgaagccc aaatgatttt gatttgcccc tcgttgtgtc tgatcaattt accccaaatt
     1261 agttgttgga ttctttggct ttataaagta taatatatta tcactaaacg ttataaatga
     1321 atgttatgga taatatatta gccattataa accaaaaccc caagaacctc atgctcgcac
     1381 tatccaaacg agcaaaagaa cggcgcttgt tttacgggta caaaagaacc acattggcaa
     1441 aaaaggccgg tgtgaacgtg gcctcgctca agcggtttga attgacaggg cagatttcgc
     1501 tggtttcact tctgaaactc gctttcgcgt tggactgtct tgctgaattt tcaaatctat
     1561 ttcctcttcc agaagcgcat tccatcaagg aactggaaca acgaatgaaa ccgatcagaa
     1621 gaaagcgggg gcatctgtga aaaagctcca cgttttttta accgatgctt ttgggcaaaa
     1681 aaaacttgtg ggcacattgg cagagttcga tcgccgaatt gtttttgaat attccaacgc
     1741 ctttctggaa gaacacattc aaatgaggaa acactgaagg cgtttcgctg gatgtgcttt
     1801 aatattgtat ttcataatcg cgatgaccac gtaaaaaact tttcctatgt gatggatgat
     1861 gatggtcgat ggaagctgtc tctggcatat gatctttgtt tttcagaagg tttgggcggt
     1921 gaacatttca tgaccgtgat gggcgagggg cgacaaattg cgcgggaaca cattctgaag
     1981 cttgccagag aaacagggat cagcgagctg ttaaaataac atgatctgaa gctgggaaag
     2041 gcttggcacc acagcatccg cctgcgagag gttctgtgcg gaggtggaac tggtgagggc
     2101 gatcactggg agacccgctt tcttggcggc agacacaccg gtgggggaat cctcaatgac
     2161 ccagcaatct tttttggtga tttggggatg atgtcttttc atgcgagtca aggcctgaag
     2221 atagccttcg ggatggggct ttgttttttg cacatcctca tgacagacca tgaactcaaa
     2281 gaaatccaaa agctcacatt ttagcagaca cgccttcacc tcggatcgca gggagcccga
     2341 tacgatcgcc aaagggacgt tgtgcatttt gagtgtttta agaagacgtg tgatgcccgc
     2401 catgcggatg tctgaggtct gcaccattcg cataaagatt gtgtgttttg atttgaggag
     2461 ctgtgcgcag aggcttcgtg agagtgtttt tcccgccaga tcaaaaaaat ggtgaaagag
     2521 cgagatgtca tccatgccgc aggtgtgttg atcataatac tgattggtga aacgtatgcc
     2581 aaattccttg agcacctcac gggcggcgcg cct
//
LOCUS       DATDUN010000429         3615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_394554, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000429 DATDUN010000000
VERSION     DATDUN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394554"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VJC18_06020"
     CDS             <1..615
                     /locus_tag="VJC18_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLD44974.1"
                     /translation="LSGGERSRVLLGKILANPVNLLLLDEPTNHLDQESIEGLLQGLE
                     VFEGAVLMVTHSEMILQRFAKRLIVFQNDEILVFEGTYDDFLRRVGWSTENTQENGDE
                     TQGRLSKKEIRQQRAKQLTQKSRVLKPLQTQVNKLEHQILKLEKEVNAATQHLIEASA
                     SNDVPKQVRLSKKLKQDETQIEILYSELDQVTREYESALAELDE"
     gene            complement(718..2022)
                     /locus_tag="VJC18_06025"
     CDS             complement(718..2022)
                     /locus_tag="VJC18_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HLD44975.1"
                     /translation="MKFPGQRKQKHYFPVSERGRIPFHTFPQNPKRVYIAGIDQLIVD
                     IEALVEDGYLERTGLHKGQSMILDTPQADGIYRELKKSGRVTGEYAGGAVCNTLHNYS
                     VLTDDKSVAFGVISKNITVGDNAYNYICNTNMHVDLSYLQPLDGPIGRAICFVTPDGE
                     RTFAIAKGRMNDLSADYINESVIASSAALLLTTFLFRDEASPIYKTTLRAIRIANEND
                     VPVVLSLGTSELVEAKKDFFRQLIRESVTVLAMNDAEGLALTGQSDPLLCLQSVLDIC
                     DFGLLTVGEKGLYLGAYVDEPLVRETEEQIMSKSIPEYNKFEFSRAMRKSCCLQKPVK
                     IYSHINPFLGGPSVIKSTNGAGDGALAALLHDMSANAYHKICVPNSPKHAAPFLTYSS
                     ICQLCKYANRVSYEIISRNSPRLTRGLPEKEDDLEEAYWDRG"
     gene            2245..3114
                     /locus_tag="VJC18_06030"
     CDS             2245..3114
                     /locus_tag="VJC18_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD44976.1"
                     /translation="MTTTATNINLYDYLDYRLFLKDWYKFSKKSRRGFSFRAFSKRAG
                     NPAPNFLKRVMDGQHNLTPNGIKEMIKGLGLNKQESDFFIVLVHFNQAKTHEEKNKQY
                     QKIIRSACFAKLRPLDKNQYLYFSTWYNPIIRELVVSSAFDGSYEWLANKIRPVITPE
                     QAKKSVALLATMGFIKENSDHTWSQTTALVSTGHEAVDISIANYHLNVLSLIAKLITR
                     IEPEQRQSHTMTLGVTRDKIALLKKKVQEFRNDVLTLVAEDTDPEEVVLVSVHMLPVT
                     QETPEKDKKLSNV"
     gene            3152..>3615
                     /locus_tag="VJC18_06035"
     CDS             3152..>3615
                     /locus_tag="VJC18_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44977.1"
                     /translation="MKMHRPAIWFYVLNISLALMLAGCGDLNQNLSDDPAPEKVIIDH
                     QQIDVLGGSDAGNPPISNLVGRTLSGTWSDNTDLPDTIIATDIDGTLYQISLEATTGA
                     GFSMTLPTGRIYTLAFVKNGLVLGQLILDNGSLGLSANYFLVSDLENKNITLG"
BASE COUNT         1018 a          858 c          859 g          880 t
ORIGIN      
        1 ctttcgggag gggaacgaag ccgcgttctg ttgggcaaga ttttggcgaa ccccgtcaat
       61 ctgctcttgt tggacgaacc cacaaaccat ctggaccagg agtccattga aggtttgttg
      121 caggggctgg aggtttttga aggcgcggtg ctgatggtga cccacagcga aatgatcctc
      181 cagcgatttg ccaaacggtt gatcgttttt cagaacgacg aaatcctggt gttcgaaggc
      241 acctacgacg atttcttgag gcgtgtgggc tggtctaccg aaaacacgca agagaatggc
      301 gatgagacac aaggcagact cagtaaaaaa gaaatccgcc aacagagggc caagcaactc
      361 acccaaaaat cgcgcgtgct caaacccctg caaacacagg tcaacaagct cgagcatcaa
      421 attctcaaac tcgaaaaaga ggtcaacgca gccacccaac acctcatcga ggcctcagcc
      481 tcaaacgacg tgcccaaaca agtcagactg tccaaaaaac tcaaacaaga cgaaacccag
      541 atcgaaattt tgtacagcga actcgaccaa gtcacccgtg agtacgagag cgcgctggca
      601 gaattggatg agtgaactga gtgacctatc attgcaagcc ggactagacc tgttttcaag
      661 acatattgtt cttaaaaagt acatgatcaa agaaaaaatg gacttgtaat ctgatcttca
      721 cccccgatcc caatacgcct cttcgaggtc gtcttctttt tcgggcagac cgcgggtgag
      781 tcgcggggaa ttgcggctga tgatctcgta gctcacgcgg ttggcgtatt tgcagagctg
      841 gcaaatggag gagtaggtca aaaacggggc ggcgtgcttg ggtgaattgg gcacgcagat
      901 tttgtgatag gcgttggccg acatgtcgtg caggagagcc gccagtgcgc cgtcccctgc
      961 cccgttcgtg cttttgatga cagaagggcc acccaaaaaa ggattgatgt ggctgtaaat
     1021 cttgacaggc ttttgcaggc aacaactttt tcgcatggcg cgcgaaaatt caaacttatt
     1081 gtactcagga atagacttcg acataatctg ttcttcggtc tctcggacca gaggctcatc
     1141 gacatacgcc cccagataca gccctttttc acccactgtg agcagaccaa aatcacagat
     1201 atccaataca gattgcagac acaacaaggg atcactctga cccgtcaggg ccaacccctc
     1261 cgcgtcgttc atggccagga cggtaaccga ttcgcgaata agctggcgaa aaaaatcttt
     1321 tttggcttcc accagttcac tggtccccaa actcaaaacc actggcacat cattttcgtt
     1381 ggcgatccgg atagcccgga gagtcgtttt gtaaatggga gaggcctcat cgcgaaacaa
     1441 aaatgtagtg agcaaaagag cggcggaact ggcaatgacc gactcattaa tgtagtctgc
     1501 cgacaaatcg ttcatccgac ccttggcaat ggcaaaggtg cgttcgccat cgggggtcac
     1561 gaaacaaatg gcgcggccaa tgggcccatc caagggctgt aaatacgaca gatccacatg
     1621 catgttggtg ttacagatgt agttgtacgc gttgtcaccc accgtgatgt tcttggagat
     1681 cacgccaaac gccaccgatt tatcgtcggt caggaccgaa taattgtgga gcgtattgca
     1741 gacagcgcca ccagcgtatt caccggtgac gcggccagat tttttgagtt cgcggtaaat
     1801 gccatcggct tgtggcgtat ccaaaatcat ggactggccc ttgtgaagcc ctgtacgttc
     1861 taaatagcca tcttctacca aggcctcgat atccacgatc aattgatcga tgccagcaat
     1921 gtaaacccgt ttggggtttt gcgggaaagt gtgaaaagga atgcgcccgc gttcgctgac
     1981 gggaaaataa tgtttttgtt ttcgttggcc gggaaatttc atggaggctc atagaacaaa
     2041 acgttctgga cactcaacaa aaagatgccc ctcctccctt gtttcttgtt ttgccaattt
     2101 cttaactttc gcaggatctt tgaaaaacaa ttgagattat tatgattttt gatacaaaat
     2161 aagttgtatc atattgatat ataatatgat tatattctat atgttgcttt ttgcctctaa
     2221 ttttgataca attatcctca caacatgaca acaacagcta cgaatatcaa tctgtatgat
     2281 tatctggatt atcgtctttt tttaaaagac tggtacaagt tttccaaaaa atcgcgtcgt
     2341 ggtttttcgt tcagggcctt ttctaagcgg gcaggaaacc ccgcacccaa tttcctcaaa
     2401 cgcgtcatgg acggccagca caacctgacc cccaacggca tcaaggagat gatcaagggc
     2461 ttggggctca acaaacagga atcggatttt tttatcgtat tggttcattt caatcaggcc
     2521 aaaacacacg aagagaagaa caaacagtat caaaaaatta tccgttcggc gtgttttgct
     2581 aaactgcgtc cgctggacaa aaatcagtat ctgtattttt ccacgtggta taaccccatc
     2641 attcgcgaac ttgttgtttc aagcgcattt gacggttctt acgagtggct ggcaaataaa
     2701 attcggccag tcatcacgcc agagcaggca aaaaaatcgg tggcacttct ggcaacaatg
     2761 ggatttatca aggaaaattc tgaccacacg tggtcacaaa caacggcgtt ggtttctacg
     2821 ggccatgaag ctgtcgacat ttctatcgcc aattatcact tgaatgtgct ttccctcatt
     2881 gccaagctca tcacgcgcat tgagccagag caaagacaga gtcacaccat gacgctaggc
     2941 gtgaccagag acaaaatagc cctgctcaag aaaaaagttc aagaattcag aaatgatgtc
     3001 ttaacgttgg ttgccgaaga cacagaccct gaagaggtgg ttttggtgtc tgtgcacatg
     3061 ctgccggtta cccaggaaac accagaaaag gataaaaaac ttagcaacgt atgaaataaa
     3121 gagcccgata ctagggcata ggtacagaat tatgaaaatg catcgcccag caatttggtt
     3181 ctacgtttta aatattagtc tggctcttat gcttgctggt tgcggagatc tgaatcagaa
     3241 tttgagcgat gatccagccc cagaaaaagt gattattgat caccaacaaa ttgatgtgct
     3301 tggcggttcg gatgccggaa acccacccat ttcaaatctt gtgggacgaa ctttaagtgg
     3361 gacctggagt gacaacacag acctgcctga cacaattatt gccacggaca tcgatggcac
     3421 actctaccag atttcgctag aggctaccac tggggctgga ttcagcatga ccctgcccac
     3481 gggaagaatc tatactttgg cctttgtaaa aaatgggctg gtgcttggac aattgatttt
     3541 ggataacggc agtcttggtc tcagtgcaaa ttactttctg gtgagtgatt tggaaaacaa
     3601 aaacatcaca ctggg
//
LOCUS       DATDUN010000430         4025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_227447, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000430 DATDUN010000000
VERSION     DATDUN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227447"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VJC18_06040"
     CDS             complement(<1..561)
                     /locus_tag="VJC18_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44978.1"
                     /translation="MKTNKTISYLLVFLFIVNVSTGCGALSGSDDNSSSEFSDMDYPD
                     AVALYATNGLSFTETMGDLATSHNTFLTTDTTTADASTLQTALTDFTDDLTSFTTLAE
                     DTNDLEVLAFEETTAASLTSGIVKFQSIDPSGVDSLNSLTDVMLAKKKECDALFALVP
                     SLPSSPSDFKKLEAAQQCAREAQILAL"
     gene            complement(683..886)
                     /locus_tag="VJC18_06045"
     CDS             complement(683..886)
                     /locus_tag="VJC18_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44979.1"
                     /translation="MCEIKYSENPVMLDKALREQIVRQKEFLSTAFRGYRLEHWLITN
                     TQVSDVVQKSDLIQRVITIQDLF"
     gene            complement(1022..1993)
                     /gene="hemB"
                     /locus_tag="VJC18_06050"
     CDS             complement(1022..1993)
                     /gene="hemB"
                     /locus_tag="VJC18_06050"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HLD44980.1"
                     /translation="MSFPTTRSRRLRSNPTMRRLVRETKLSVDDVIMPIFLTFGKNKK
                     EAISSMPGVYRYSIDRVDEEIECLTSLNIPGTILFGIPEHKDPQGSGAYDENGIIQQG
                     IRHIKTLAPDLLVIADLCLCEYTSHGHCGMIKGDQILNDESLAVLAKTAVSQAHAGAD
                     IIAPSDMMDGRVRAVRLALDENGFSNTPILSYAAKYASSFYGPFREAAQGAPQFGDRK
                     TYQMDPANAREAIKEILQDIEEGADMIMVKPALPYLDIIAKAREINTLPVCAYQVSGE
                     YAMIMSAAQNGWLDKDKVMLETLTAIKRAGADFIITYFAKDFAKMIC"
     gene            2200..2760
                     /gene="msrA"
                     /locus_tag="VJC18_06055"
     CDS             2200..2760
                     /gene="msrA"
                     /locus_tag="VJC18_06055"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HLD44981.1"
                     /translation="MTDHVNLKLATFAGGCFWCMEGPFEALKGVTQVIAGYAGGHKQN
                     PTYEEVRSGETGHLEVIQVTYDPGVVSYEHLLQVYWRQIDPTDDCGQFHDRGVQYQTA
                     IFFHDEEQKHQAEQTKQALEREHIFSKPIVTQILPYSNFFCAEEYHQHYYRKNPQQYE
                     AEVHTSGRKSFLQKIWPKKTTGHGKW"
     gene            complement(2767..2940)
                     /locus_tag="VJC18_06060"
     CDS             complement(2767..2940)
                     /locus_tag="VJC18_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLD44982.1"
                     /translation="MKFNQSGFTKKELLIVLGTITIMCLASLPLYYKLHKNIHSKHIH
                     NKIERVNTIHVTK"
     gene            3326..>4025
                     /locus_tag="VJC18_06065"
     rRNA            3326..>4025
                     /locus_tag="VJC18_06065"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1057 a          839 c         1042 g         1087 t
ORIGIN      
        1 gagggccagg atttgagcct cccgtgcgca ttgttgtgcg gcttccagct ttttgaaatc
       61 ggagggagaa gatggcaaag acgggacaag cgcaaacagg gcatcacact cctttttctt
      121 ggcaagcatc acatcggtga gggaattcaa agaatccaca ccactggggt caatgctctg
      181 aaatttgaca ataccggacg tgagtgatgc cgccgttgtc tcctcaaaag ccagaacttc
      241 gagatcattt gtgtcttcgg ctaacgtggt gaacgatgtg agatcatctg tgaaatcagt
      301 cagggctgtc tgcagggtgc tggcatctgc agttgtggtg tccgttgtta aaaacgtgtt
      361 gtgggatgtg gcgagatcac ccattgtctc tgtaaaactt aatccgttgg tggcgtaaag
      421 agcaaccgcg tcgggatagt ccatatcgga aaattcactg gaagaattgt catcagatcc
      481 ggatagggca ccacagccag ttgagacatt cacaataaaa aggaacacca gtagataaga
      541 aattgttttg tttgttttca tgtgaagaga tattagtaca aggaacagca aggtcaataa
      601 ataaagtctg tgaaaggatc ggcttgtgac taggtggatt gatgtcacat cgttcttgcc
      661 acacctgaat aagtcggaat tgtcaaaaca aatcttgaat ggtgatgact ctctgtatca
      721 ggtcggattt ttggacaaca tcactcacct gtgtgttggt gatcaaccag tgttcgaggc
      781 gataacctct gaacgcggtg ctcaaaaatt ctttttgtct cacaatctgt tcccgcaaag
      841 ccttgtccaa catcacagga ttttcagaat attttatttc gcacacggtg agcgttttgt
      901 cggctcggac atgaacctag ccataaatca atcttaattt gacatttatg gctactttaa
      961 atcagctaaa cttagccaca gatcaggttt tcgcatggcc ctcaataacc caaaaaaatg
     1021 tctaacagat catttttgca aaatccttgg caaaatacgt gatgatgaag tccgctccgg
     1081 cgcgcttgat ggcggtgagc gtctcgagca tgaccttgtc cttgtcgagc cagccatttt
     1141 gggcggccga catgatcatg gcgtactcac cgctgacctg ataggcacaa actgggagag
     1201 tgttgatttc tctggctttg gcgatgatgt caagataggg aagggcaggt ttgaccatga
     1261 tcatgtctgc gccttcttca atatcctgca aaatttcttt aatggcctca cgtgcgttgg
     1321 cagggtccat ctgatacgtc ttgcggtctc caaactgtgg ggccccctga gccgcctccc
     1381 gaaagggacc ataaaacgat gatgcgtatt tggcagcgta agataaaatg ggcgtgttgg
     1441 aaaaaccatt ttcatcgagg gccaagcgca cggcgcgtac ccggccatcc atcatgtcgg
     1501 atggcgcgat gatgtcggct cctgcatggg cttggctcac ggctgttttg gcgagtaccg
     1561 ccaagctttc gtcgttcaga atctgatccc ccttgatcat gccacagtga ccgtgactgg
     1621 tgtattcaca caggcacaaa tcggcaatca ccaacagatc gggggcaagt gttttgatgt
     1681 gtctgatccc ttgctgaata atgccatttt catcgtaagc ccctgaaccc tgtggatcct
     1741 tatgttccgg gatgccaaac aggatggtgc ctgggatgtt aagtgatgtt agacattcaa
     1801 tttcttcatc cacccggtca atgctgtagc gatacacgcc cggcatggac gagatggctt
     1861 cttttttgtt ttttccaaat gtcaggaaaa tgggcatgat cacatcgtcg acgctcagtt
     1921 ttgtttcgcg aacaaggcga cgcattgttg ggttggacct caaacggcgt gagcgggttg
     1981 tgggaaagga catagaagct ctcctttgaa tttacgtatg tcactaatgc gataataata
     2041 caagtttggg acatacgctc aaagctcaaa gctcaaagga aagaatccat tacattctga
     2101 gaatgatatc attttttcat tgcttcattg cttgattgct tcattgcttg attgcttcat
     2161 tgcttgattg cttctccccc ttcatattat gcaggtcgaa tgacagatca tgtgaactta
     2221 aaactggcga cgtttgctgg cggttgtttt tggtgcatgg agggcccttt tgaggctctg
     2281 aaaggcgtga cgcaagtgat tgccggctat gccggtggac acaagcaaaa cccaacttat
     2341 gaggaagtgc gctccggtga aacggggcac cttgaagtga ttcaggttac gtatgatcct
     2401 ggtgttgttt cctatgagca tcttttacag gtgtactgga ggcagatcga tcccacggat
     2461 gactgtggcc aatttcatga tcgcggtgtg caatatcaga cggccatttt ttttcacgac
     2521 gaggaacaaa agcaccaggc agaacagacc aaacaggcct tggagcggga acacatattc
     2581 tcaaaaccca tcgtgaccca aattctaccc tatagcaatt tcttttgtgc agaagaatat
     2641 catcaacatt attacaggaa gaaccctcag caatacgaag ccgaagtgca tacatcaggt
     2701 cgcaaaagct ttttacaaaa aatctggccg aaaaagacca cagggcacgg taagtggtag
     2761 tggtttctat tttgtgacgt ggatggtatt tacccgctct atcttgttgt gtatgtgttt
     2821 gctgtgaatg tttttgtgga gtttgtaata gagtgggagg gaggccaagc acatgattgt
     2881 gattgtaccc agcacaatga ggagttcttt tttggtgaaa cctgattggt taaatttcat
     2941 aggggcaaac aattagtggg tgtgggcatg aaaatcaaag agtttacaat gaaagaaatc
     3001 atataatttt taagtaattt tgattgacac caattttttt tcatgtagta taagcgaact
     3061 gtctgcaaat gaacatttag ggtcctcttt gcggcgggtg gcttcttcag gagttgccca
     3121 aaaaaatcaa aaaattttta aaattttgct tgcgctccaa aaaagacgat gttaagtgcg
     3181 tcgtccggtc aggagactgg ttctttgaaa attaaataac agaatgacta ataagtatga
     3241 gcgggcttta tatcgtcgta agatgatgta aagaccttac aatttttttg ggtctcgttt
     3301 ttaacgggac tgcagatcaa gctttcaact ggagagtttg attctggctc agaacgaacg
     3361 ctggcggcat gcttaacaca tgcaagtcgt acgagaaagt tcccttcggg gaatgagtaa
     3421 agtggcgcac gggtgagtaa cacgtagata atctacccta tagacgggga caacagatcg
     3481 aaaggtctgc taactcccga tattctcttt gaatcgtaaa gatttgttga ggaaagacag
     3541 ccctctgatc taaggttgtg ctataggatg ggtctgcggc ccatcagcta gttggcgagg
     3601 taatggctca ccaaggctat gacgggtagc tggtctgaga ggacgatcag ccacactggg
     3661 actggtacac ggcccagact cctacgggag gcagcagtgg ggaatattgg acaatggggg
     3721 aaaccctgat ccagcgatgc cgcgtggagg aagaaggcct ttgggttgta aactcctgtc
     3781 ataggggaag aagataatga ggtgaacaat cttattgttt gactgtaccc taagaggaag
     3841 caccggctaa ctccgtgcca gcagccgcgg taatacggag ggtgcaagcg ttgttcggaa
     3901 ttactgggcg taaagcgtgt ccaggtgggt ctgtaagtca ggtgtgaaat cccccagctt
     3961 aactggggag ggtcgcttga gactgcagat cttgagtttc ggtgaggtgg gtggaattcc
     4021 cagtg
//
LOCUS       DATDUN010000431         1309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_604327, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000431 DATDUN010000000
VERSION     DATDUN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604327"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1309)
                     /locus_tag="VJC18_06070"
     CDS             complement(<1..>1309)
                     /locus_tag="VJC18_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44983.1"
                     /translation="PVPEIILPSVPTPPTIPTGQNYLPVGGNIHRLPDTQRYFWDVDN
                     KRIGYRITNARDDMRQATIGRPPIPEGTKEVFTFLGNPYPASARLRAFETLARMFGPQ
                     GRSATEFYESIVQIPMMYHRGEWIPDPTRKAYVQVNRPILDTDRVTGNYRDSNSGEFA
                     FTLKEGTADTVIEIARPEGDPINLTVFRTIVPIREEDKARLKATTEFTPEFGIALGEP
                     TQKSHEIMTRIFNATGGYDAIVEALTRRPRPDMRVDYTRPGLDTTVLVPKIGIPIYTA
                     HESVRSRGTVVELRPGYSTRYPDDRFAQLSLKDGNGLNIKMILHHPYGRTMPVVASFE
                     SAGLPTDPRELSFTRHGNTVIIGGYTNYRTYRAGGGTEIKSLPFVIEGEVNNGTFAIK
                     KITIDQEDITPEQFAYAPRIYDEQTVENLQYTEIRIGNLFRRTS"
BASE COUNT          262 a          309 c          398 g          340 t
ORIGIN      
        1 ccgatgttct tctaaaaagg ttgcctatcc tgatttcagt gtattgcagg ttctcaacgg
       61 tttgctcatc gtagattctc ggcgcatagg caaattgctc tggcgtgatg tcttcctgat
      121 caatggtgat ctttttgatg gcaaacgttc cattgttcac ctcgccctcg ataacaaagg
      181 gaagtgattt gatttcggta ccgccaccgg cccggtaggt gcggtagttg gtgtaaccac
      241 cgatgatgac cgtattgcca tggcgagtga aggaaagttc acgcgggtct gtggggagtc
      301 ccgcggattc aaacgaggcc acgacgggca tggttctgcc atacggatga tgcagaatca
      361 tcttgatgtt gagcccattg ccgtctttga ggctgagttg cgcaaagcgg tcatcggggt
      421 agcgcgtgga ataacctggc ctgagttcca ccacggtccc acgagaacgc acgctttcat
      481 gtgctgtgta aatgggaatg ccaattttgg gcaccagcac ggtggtgtcc aaccccgggc
      541 gcgtataatc aacgcgcata tccgggcggg gacgtctggt gagggcttcc acgatcgcat
      601 catagccccc tgtggcgttg aagatgcggg tcatgatctc gtgactcttt tgcgtgggct
      661 caccaagggc aatgccaaat tcgggtgtga attcggtggt ggctttgagg cgtgccttgt
      721 cttcctccct gatgggcaca atggttctaa aaacagtcag gttgatgggg tcaccctctg
      781 gccgtgcaat ttcgatgacc gtatcggccg tgccttcctt gagtgtaaac gcgaattcac
      841 cgctgttgct gtcgcggtaa tttcctgtga cgcgatcggt atccagaatg ggtctgttga
      901 cctgcacata ggccttgcgt gtggggtcgg ggatccattc gccgcggtga tacatcatgg
      961 ggatctggac gatggattca taaaattctg tggcactgcg accttgtggg ccaaacatgc
     1021 gggcgagtgt ttcaaatgcc ctgagtctgg cgctggcagg gtaggggttg cccaaaaagg
     1081 tgaatacctc tttagtccct tccgggatgg ggggacggcc aatggtggcc tgtcgcatgt
     1141 catcacgggc attggtgatt cgatatccaa tacgtttgtt gtccacatcc cagaagtaac
     1201 gctgtgtatc gggcagtctg tgtatgttgc cgccgactgg caaataattt tgtcccgtgg
     1261 gaatggtcgg tggtgtggga acagatggca aaatgatttc gggaacagg
//
LOCUS       DATDUN010000432         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_311020, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000432 DATDUN010000000
VERSION     DATDUN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311020"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VJC18_06075"
     CDS             complement(<1..161)
                     /locus_tag="VJC18_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HLD44984.1"
                     /translation="MKTVLLMGNPNVGKSALFSRLTGTQVVISNYPGTSVEYTQGALK
                     HETEEATII"
     gene            complement(158..394)
                     /locus_tag="VJC18_06080"
     CDS             complement(158..394)
                     /locus_tag="VJC18_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HLD44985.1"
                     /translation="MTLKTLIDMKPGESGMVVEITGGPGCLKQLNNLGLNAGKIIHKI
                     GAHLWRGPQIVKLGQIQIVVGHGIAQKIKVETDQ"
     gene            complement(391..777)
                     /locus_tag="VJC18_06085"
     CDS             complement(391..777)
                     /locus_tag="VJC18_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HLD44986.1"
                     /translation="MNRSLSLSSHNEDYLETIAALKKKYGWARVKDISAALHVKASSV
                     NSAIKLLSQNGFVEYERYGRIELSEKGKQKARAIQKRHDLLTRFLTQVLRVSPKTATL
                     DACKMEHVISIQTFEALVRFMQEHTP"
     gene            1082..>2549
                     /locus_tag="VJC18_06090"
     CDS             1082..>2549
                     /locus_tag="VJC18_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44987.1"
                     /translation="MNYHKINLLIFSFVLVFLFSLTSACAPDSKLVLPAAEQHTDNDI
                     AEDDNLDTPETEIVSAPDTNISVPLGGDSETTIENTNETATDTVTVKESGEEANTGIQ
                     FKKNQKQKQKKEKKEKKEKKIKIRPKQKKHPAEQAQEQPQEQSQEQPQEQPQKQPLPQ
                     TADLGVSNDPETSTSTVTGTDTDTSTDTGTGSESVQETSLVVDDCYSKKTLPCDGSSA
                     YNDPACYISSLKADLRKAQENSQMIYVAISQEAYQAQESTKSCPWSLVNGQCPWGSDT
                     HDGLSRNTALATFYQALYTMYELQNHADDKNSHFVLKIVGGDYYYREPIIISAKAGLL
                     GKIYLGPRLYENGQPVKKNNKGEYVVWDPSGNDNAEYNSLVSPTDLVIANITVEPYDQ
                     KPVTLDGGCRFEYRTLADGTILNPDHPDYVWGYSYLNRTCDLDLCTAEELEYRNKRLE
                     DPVNTPFDGILSYGFCNGRGMVNVIKGDNIEVKKLNIQN"
BASE COUNT          719 a          568 c          615 g          647 t
ORIGIN      
        1 tcaatgatgg tggcttcttc ggtttcgtgt ttcagtgccc cttgtgtgta ttcaacagag
       61 gtgccggggt agttggaaat caccacctgt gtgccggtga gacgggaaaa cagggcggac
      121 ttgcctacgt tggggttgcc catgaggaga acggttttca ctggtcggtc tccaccttga
      181 ttttctgggc aatgccgtga cccacaacga tttgtatttg acccaatttg acgatctggg
      241 gcccgcgcca caaatgcgcc cctatcttgt gaatgatttt acccgcgttg agccccaggt
      301 tgttgagctg tttcagacaa ccgggaccgc ccgtaatttc cacgaccatc cccgattcgc
      361 ccggtttcat gtcgatgagg gttttgagcg tcatggggtg tgctcctgca tgaaacgcac
      421 gagggcctca aaggtctgga tgctgatgac gtgttccatc ttgcaggcat cgagcgtggc
      481 tgttttggga ctgaccctga ggacttgggt gaggaaccgc gtgagcaagt cgtgacgctt
      541 ttgaatcgca cgggcctttt gtttgccttt ttcggagagt tcgatccgtc cgtagcgttc
      601 gtattccaca aaaccatttt gggaaagcag cttgatggcg ctgttcacgc tggaggcctt
      661 gacgtgcagg gctgccgaga tgtccttgac tcgcgcccag ccgtatttct ttttcaaggc
      721 ggcgatggtt tcgagatagt cctcgttatg tgaagacaaa gacagggacc gattcacaaa
      781 gagctccttt gttttgatgc tctaacagag ttagttaagt ctaacaaaat ggatcaggat
      841 ttgtcaagga tccggagcga aaactcaagg cttgtttggg acagacggcc agacacttga
      901 atttgatcaa aatgataaaa cagagggcct ttgtgccatt atttatatgc ctcatttgcc
      961 ctgattgagc ggcgacccgc taacctgttg ttataatgcc atttttttta atgttttaca
     1021 gcaaagcgtg gcattcattt tgcgtacaac attggggatg gggaatgaaa tgaggtgtta
     1081 gatgaattat cacaaaatca atttactaat tttcagtttc gtactggtat tcttgttcag
     1141 tctgacctcg gcttgtgcac ctgattccaa gttggtgttg cctgctgccg agcaacatac
     1201 cgacaacgac attgctgaag atgataatct tgacacccca gaaactgaga ttgtttctgc
     1261 tcccgatacc aatattagtg tcccactcgg tggtgattca gaaacgacaa tcgaaaacac
     1321 caatgaaaca gcaacggaca cggtgactgt taaagaatca ggagaagagg ctaacacagg
     1381 gattcagttc aagaaaaatc aaaaacaaaa gcaaaaaaaa gaaaagaaag aaaaaaaaga
     1441 aaagaaaata aagatacgac caaaacaaaa aaaacatcca gcagaacaag ctcaagagca
     1501 gcctcaagag caatcacagg agcaaccaca agaacagcca caaaaacaac cattacctca
     1561 aacggctgac cttggcgtca gcaatgaccc cgaaaccagc accagcactg ttactggcac
     1621 tgacactgac actagcactg acactggcac tggttctgag tcagttcaag aaaccagtct
     1681 tgttgttgat gattgttatt caaaaaaaac gctgccctgt gatggttctt cggcctacaa
     1741 tgaccctgcc tgctatattt cttctctaaa agcagatttg agaaaggcac aggaaaattc
     1801 acagatgatt tatgtcgcta tctcgcagga ggcgtatcag gcacaggaaa gtacgaaatc
     1861 gtgtccatgg agtcttgtca atggtcaatg tccttggggg agtgacacac atgatggcct
     1921 ttcgcgcaac acagctctgg ccacttttta tcaggccctc tacaccatgt atgaattgca
     1981 gaatcatgcg gatgacaaaa atagtcactt tgtactaaaa attgttggcg gagattacta
     2041 ttatcgtgaa cccattatca tatctgcgaa agcgggattg ttgggcaaaa tttatttggg
     2101 gccgcgtctg tatgaaaacg gacaaccggt caaaaaaaat aacaagggtg agtatgttgt
     2161 ctgggatcct tcagggaacg ataacgcaga atacaacagc ctcgtgtcgc ccacagatct
     2221 tgtcatagcc aacatcacag tcgaacccta tgatcaaaaa ccggtgacct tggacggtgg
     2281 gtgtcgcttc gaataccgca ctctggcaga tggcacgata ctcaatcccg atcatcctga
     2341 ttatgtatgg ggttattcat atctaaacag aacttgtgat ttggatttgt gcaccgcgga
     2401 agaactggaa tacagaaaca aaagactcga ggaccctgtg aatactccgt ttgatggcat
     2461 cttatcatat ggtttttgca atggtcgcgg catggtcaat gtcatcaagg gtgataatat
     2521 tgaagtaaaa aaactcaaca tacaaaacg
//
LOCUS       DATDUN010000433         2992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_270453, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000433 DATDUN010000000
VERSION     DATDUN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270453"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VJC18_06095"
     CDS             <1..348
                     /locus_tag="VJC18_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HLD44988.1"
                     /translation="RRFAVILERDADKWFKPAKGQFQLFYKLGVEQPEYVPDFVVETA
                     HHLLMCETKARNELGADDVKAKAEAGTLWCKHASDHARTTGAKPWKYLLIAHDVVTED
                     KRLVDFLPFEEIS"
     gene            complement(457..816)
                     /locus_tag="VJC18_06100"
     CDS             complement(457..816)
                     /locus_tag="VJC18_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44989.1"
                     /translation="MIDIDKAMLVLVLAALDPNFLPPYSDSLIKQAKELDLLIERMNW
                     TTGMYFGPVVGQDDQAWLIKIGTGRASVLPFCEIEEGAVIPRLGDNILLTLTHGLVHV
                     VVFPSSENNSEDEKRLD"
     gene            complement(809..1042)
                     /locus_tag="VJC18_06105"
     CDS             complement(809..1042)
                     /locus_tag="VJC18_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44990.1"
                     /translation="MKHPLDSLRSSATVISAAALVTDSKGSLWHQNHGVTMPVTVQTK
                     LLGLIVATSQQQAKTHNRVAGIHLFLSLGNCHD"
     gene            complement(1039..1527)
                     /locus_tag="VJC18_06110"
     CDS             complement(1039..1527)
                     /locus_tag="VJC18_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44991.1"
                     /translation="MDPENARALLLRRGIELARNDPLLTFVLLNDVVLKGVLNPMFAA
                     DTKDMQAILRDKGIRISDDDPIFALLALNGIVLYHSIDKVRRAQVKIGSASRSAIYLK
                     TLTATAFVVGFGVGVFFGLNHIETSLVFSAVIGCVLGIALGALGVVLVDKTNFQYKRK
                     TK"
     gene            complement(1530..2261)
                     /locus_tag="VJC18_06115"
     CDS             complement(1530..2261)
                     /locus_tag="VJC18_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HLD44992.1"
                     /translation="MNNVHLVMQGKGGVGKSLAAVLVAQYLQSLGGKIICADTDPVNA
                     TFAQYQSLDVAHIEIAKDGNVIQSKFDPLIEMIVQTDADFVIDNGAATFLPLTKYLAE
                     NDIYQIMSEHGKKVFVHSVLTGGQAKTDTYNGFAELLGKVNQFAKVVVWENEFWGPVE
                     YEGHPITATKLFKESDRAGKIAGVVKLVDRSQSDAFVEDMKKMTSNHMTLADVNASED
                     FNFLAKNRLKKIVDAVFEELDRVKW"
     gene            complement(2278..2679)
                     /locus_tag="VJC18_06120"
     CDS             complement(2278..2679)
                     /locus_tag="VJC18_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44993.1"
                     /translation="MDANEFLRNVTPGVRRSRLAPYWNEINKLRNGNCTLAQVCEFLD
                     ENDIHISIAGLAQYIKRRQHNEGKGGSQEAANLPATTPNQQPRSDTSMTASKSALVMA
                     QDSKNPLRVLSGSRKPGEFNPVPTAKIEFEE"
BASE COUNT          724 a          754 c          709 g          805 t
ORIGIN      
        1 cgccgctttg ccgtgattct ggagcgcgat gcggacaagt ggtttaaacc cgccaagggc
       61 cagttccagt tgttttacaa actgggcgtg gagcagcccg agtacgtgcc cgactttgtg
      121 gtggaaaccg cacaccattt actgatgtgc gagaccaagg cacgcaacga attgggtgcg
      181 gacgatgtca aagccaaagc cgaagcgggc actttgtggt gcaaacatgc ttcagaccat
      241 gcccgaacga ccggcgctaa accgtggaaa tacttgttaa ttgcgcacga tgtggtgaca
      301 gaagacaaga gattggtgga ctttttgcct tttgaagaaa taagttgagg gtacggttga
      361 gatggttttt ccggttaaat agcctgagag gcgaagtaga ccaacttgca tcatgaaagc
      421 tccctcgggg cgtttcaaag tgttcggggt agcaacttag tccagtcgtt tctcgtcctc
      481 gctgttattt tcgctagatg gaaagacgac gacgtgaacc aatccgtgag tcaaagttag
      541 cagtatgttg tcccccagcc gtggaatcac cgcgccttct tcaatctcgc aaaacggtaa
      601 aacactagcc cgtccggtcc caatttttat caaccaagct tggtcgtcct ggcccaccac
      661 cggcccgaaa tacattccag tcgtccagtt catcctttcg atcaataaat ccaattcctt
      721 ggcctgcttt attagtgaat cggaataggg gggcagaaaa ttgggatcga gggcggctag
      781 cacgagtacc agcatggctt tatcaatatc aatcatggca atttcccagt gaaaggaaaa
      841 gatgaatgcc agcgactctg ttgtgagttt tggcttgctg ctggctggtt gcgacaatca
      901 gtcccaacaa tttggtttgt actgtgaccg gcatcgtcac accatggttt tgatgccaaa
      961 gcgagccctt tgaatctgtg accaacgctg ctgctgaaat gactgtcgcg ctggagcgca
     1021 gactatctaa tggatgtttc attttgtctt tctcttgtat tgaaaatttg ttttgtcaac
     1081 gagtactacg ccaagggcac cgagtgcaat ccccagcaca catccaatga ctgctgaaaa
     1141 aaccaacgaa gtctcgatgt gatttaggcc gaaaaacacc ccaacaccaa agccaacgac
     1201 aaacgctgtt gctgtcagcg ttttgagata gatcgctgat ctcgacgccg atccgatctt
     1261 gacttgggct cgtcgaacct tgtcgattga gtggtacaga acgatgccgt tgagcgctaa
     1321 caacgcaaag atcggatcgt catcactgat ccggatccct ttgtcacgca gaatggcctg
     1381 catgtctttt gtatctgcag cgaacatggg gttgagcacc cctttcagca caacatcatt
     1441 cagcagaacg aaggtcaaca gagggtcatt gcgggcgagc tcaatgcctc gcctaagtag
     1501 taacgcgcgt gcattttcag gatccatggt taccacttga cccgatcaag ctcttcaaaa
     1561 actgcatcca caatcttttt cagacgattt tttgccagaa agttgaagtc ttccgaggcg
     1621 ttcacgtccg ccagcgtcat gtgattcgaa gtcatttttt tcatgtcttc cacaaacgca
     1681 tcggattgac ttctgtcaac aagcttgacg acgccagcaa ttttcccggc cctgtccgac
     1741 tccttaaaca gcttagtcgc agtaatcggg tggccttcgt actcaaccgg accccagaac
     1801 tcgttttccc agaccacgac tttggcgaat tggttaacct tgcccagcag ttcggcgaag
     1861 ccgttgtacg tgtctgtttt ggcttgccca cccgttaaga cagagtgaac aaacaccttt
     1921 ttcccatgct cagacatgat ctgatagatg tcgttctcag ccaagtactt ggtcaatggc
     1981 aggaacgtcg cggccccgtt gtcgatgaca aagtcagcat cggtttggac aatcatttca
     2041 atgagtggat caaacttgct ttgaatgacg ttgccatcct tggcgatctc gatgtgagcc
     2101 acatccaaag actgatattg cgcaaaggtc gcattcactg ggtcggtgtc ggcgcaaatg
     2161 attttgcccc ccagactttg taggtactga gctaccagaa cggccgccag cgatttgccc
     2221 acgccgcctt tgccttgcat aaccaaatga acgttgttca tattttttcc atctttccta
     2281 ttcttcaaat tcaatttttg ctgttggtac tgggttgaat tcacccggtt ttcgacttcc
     2341 tgacagcact ctgagcgggt tcttgctatc ctgggccatg accagagcgc ttttcgatgc
     2401 agtcatggag gtgtcactcc tcggctgctg gttgggtgtg gttgcgggaa gattggcggc
     2461 ttcctgagat ccacctttcc cttcgttgtg ctgacgacgc ttgatgtatt gagcgagtcc
     2521 agcaattgaa atgtgaatgt cgttttcgtc tagaaactcg cacacctgag ccagggtgca
     2581 attgccattt ctgagcttgt ttatctcgtt ccaatagggt gcaagacgcg accgcctgac
     2641 ccccggggtc acatttctca aaaattcatt tgcatccacg ttccaccttc tttgcttgtt
     2701 aagcaaatta gatcgaatat tctgggtgct taggaattgt ttttataagt taacacctaa
     2761 ttaacacttg atgtcaacaa atgtatgcaa atatgttgtt taaatgtgaa tttaaactca
     2821 tctaagctca ctaaacacct ttaaacgtcg ttaaacactt tggcattttt aaaacagagg
     2881 cacgatagcc atgtttgcaa aaactcttgc ctaacggcaa agtttgtgca atcatggcct
     2941 gtgctaaggg ggattccccc ttaaccccca gcgttgcacg ccccctccgg ag
//
LOCUS       DATDUN010000434         5736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_146103, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000434 DATDUN010000000
VERSION     DATDUN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146103"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..695)
                     /locus_tag="VJC18_06125"
     CDS             complement(195..695)
                     /locus_tag="VJC18_06125"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HLD44994.1"
                     /translation="MRNTFIPAAVLIPVFADKTNELRLIFTRRSEKVQHHKKQISFPG
                     GMVDVQDHNLWKTALRETEEEIGIKPHQIFYVCELPSIKTISQFEVTPFVGFIHSDFQ
                     IIPNADEIDTVFDAPVEHFLRPESVHHEEIEFSGERIAFPHYYYNDHEIWGATGRILK
                     SLLDRW"
     gene            802..3114
                     /locus_tag="VJC18_06130"
     CDS             802..3114
                     /locus_tag="VJC18_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HLD44995.1"
                     /translation="MNFEKYIQSYHKSIPVVSAMAVVALASEGATVPFMARYRKEATG
                     NLDEVKIKEVLDAQETWDAIIKRQQFVVGEIEKQGKLTDTLKAAILTCFDQTRLEDLY
                     LPYKQKKKTKATVAKEAGLEPLAMWILEQASLGKRAVSLTEKAAEFIQPDKKITDASL
                     AILGAQHIITELAAEDPALRQFVRDSLWKKAAIKTGKGEGAKEHSKFEPYFEFQAALS
                     SLIKPENSHRYLALRRGWVEKELELTFADIDDAQNVEESLLVAYQTKQCRHPLDDDIT
                     KIVHGACKLALRAHVLPSIHTEIHKELKDSADAAAIEVFSNNVKQLLLSAPFGAKTVL
                     GIDPGQRTGCKVVVMNAEGSVLCHDVFYLLQDNDKAKSAHKLLELVKKHGVQAVAIGN
                     GTAGRETEEFVKIIFKAQKITLPVVMVSESGASVYSASDVAREEFPDLDVTVRGAISI
                     ARRLQDPLSELVKVDPKSIGVGQYQHDVSQTQLKKKLDFVVDSCVNTVGVNVNTASYH
                     LLAHVSGIGPVLAKTLVAYRQEHGLFTSRKSLLKAPRFSKKVFEQAAGFLRIPQSKNP
                     LDNTGVHPEKYSLLETFSKSLNKTMKDFLFDGVKCLESSEEFKQQVGLFTFNDIIEEL
                     KKPGRDPREVYEVFSFREDIHELSDLSEGLVCPGIVTNVTNFGAFVDIGVHQDGLVHI
                     SQMSEAFVKDPKDVVSPGDKVAVKVISINQDKRQIGLSLRLSEAPVKKQMPQPQQTRQ
                     IVSSKGRTNAGQQSFSNNPFASLGSQLRGK"
     gene            complement(3205..4443)
                     /locus_tag="VJC18_06135"
     CDS             complement(3205..4443)
                     /locus_tag="VJC18_06135"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HLD44996.1"
                     /translation="MNQFDVIILGAGAAGLMCAAQASQRGRKVLVIDHQPKPGAKILI
                     AGGGRCNFSNLSISPENYNSQNRPFCISALKRFPPQAFLALMKKHDIAYETKNETQLF
                     CKRSSRELLNALLAECGVVQFKFGYKIQTVDPSTPSTILRTGRFTRSGQALPQQFTPK
                     FVVNTNQGEFIAKSLVVATGGITYPQLGATDLGLKIAKQFGHHIITPRPALTSWEMSK
                     RDRALFGTLAGISLPVKISFGIKCYSDSLLFTHTGISGPAVYNISLYAPAGDELKINL
                     CPALNLLEHLTTQKTKRPQAFVKTVLSELLPQRLVLTVNNHWFVDKPLTGVSKNELEQ
                     IAQAFACWKLSSEKSTDLKTAKVMAGGVDTRELSSKTMESLKVKNLYFVGEVMDVTGE
                     QGGYNLHWAWASGFCAGQFA"
     gene            4617..4838
                     /locus_tag="VJC18_06140"
     CDS             4617..4838
                     /locus_tag="VJC18_06140"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD44997.1"
                     /translation="MDLKNNYRRITLKTVEGTCINGKINIQNYNRVSDVFAASDAEFV
                     VLVDAKYCEAAPKTMFVNKQHIVWVEPEE"
     gene            4947..>5736
                     /locus_tag="VJC18_06145"
     CDS             4947..>5736
                     /locus_tag="VJC18_06145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD44998.1"
                     /translation="MSSSTTSSTAKMPKGIPFIIGNEAAERFCYYGMRAILVVYMTQY
                     LFGMNGQLAHVDEPTAKGWYHLFCSAAYFTPLIGAIISDVFWGKYKTIMRLSIVYAIG
                     CFVLALVSNTAGMVVGLTLIAIGSGGIKPVVSANVGDQFTKENQHLIKKVFSVFYFAI
                     NFGSFFSTLLTPLLLECYGPKVAFSVPGFLMLLATLVFWIGRTRFTVMPPSGIKQYKD
                     ALTSPEGKKALLHLSGFFLIISFFWMLFDQSSAAWILQADRMDRV"
BASE COUNT         1499 a         1345 c         1466 g         1426 t
ORIGIN      
        1 catgttgagt tatctgatat aacaccgtca ttcaccgccg tatcaaagtt ttgggtcaaa
       61 ttttgacttg ggacaaatgc taggctgatc gactgtgctg agaaatagaa agtaaggact
      121 tgaccccttt ccaatacaga aattatcaaa atgaatatgg gtggcacttt ttcgtccgat
      181 tgttgactgg caccttacca ccggtccaac agactcttga ggatgcgacc ggtagccccc
      241 cagatttcgt gatcattgta atagtagtga gggaaggcaa tgcgctcacc ggagaactcg
      301 atttcttcgt ggtgaaccga ttcggggcgc agaaaatgct ccacgggggc atcaaagact
      361 gtgtcgatct catcagcatt gggaatgatt tgaaaatccg aatgaatgaa tcccacaaac
      421 ggggtcactt cgaattgtga aattgtctta atgctgggca gttcacaaac ataaaaaatt
      481 tggtgtggtt tgatcccgat ttcctcctca gtctctctca gggcggtttt ccacaaattg
      541 tgatcttgca cgtcgaccat cccgccggga aaggaaatct gttttttgtg atgctggacc
      601 ttctcactgc ggcgtgtgaa gatgaggcgc agttcgttgg tcttgtcggc aaagacaggt
      661 atcaggacgg cggctgggat gaaggtgttg cgcatagaaa ttattttggt gattaaccat
      721 aacaaaaaaa aatattgaga acaatgaaaa ccacaaaaac catttgacaa gaaaggtctt
      781 tgatcccaaa agctgttccc tatgaatttc gaaaaatata tccaatcata tcacaaaagc
      841 attcctgtgg tgtccgcaat ggccgtcgta gcgctggcaa gcgagggggc aacagttccc
      901 ttcatggcgc gttaccgcaa ggaagccaca ggcaatctgg acgaagtcaa aatcaaggaa
      961 gtcctcgatg cccaggagac ttgggacgcc atcatcaaac gtcagcagtt cgttgtgggc
     1021 gaaatcgaaa aacagggaaa actcaccgac acgctcaaag cggcgatcct gacctgtttc
     1081 gatcaaacca ggctcgaaga cctgtatttg ccatacaagc aaaagaaaaa aaccaaggcc
     1141 actgttgcca aggaggcagg tctggagcca ctggccatgt ggattctgga acaggcatca
     1201 ctgggcaaac gggctgtatc actcacagag aaagccgcag aatttatcca gcccgataaa
     1261 aaaatcacag acgcctcgct ggccattttg ggtgcgcaac acatcattac agagttggcc
     1321 gccgaagacc ctgctttgcg gcaatttgtt cgtgatagtt tgtggaagaa agccgccatc
     1381 aaaactggca agggggaggg tgccaaggaa cacagcaaat ttgaacccta ttttgaattt
     1441 caggccgctc tttcgtctct gatcaaacca gaaaattccc atcgctatct ggccctgcga
     1501 cgtggttggg tcgaaaaaga actcgagctg acctttgcgg acatcgatga tgcccaaaat
     1561 gtcgaggaat cacttctggt cgcgtatcaa accaaacagt gccgccatcc tctagacgac
     1621 gacatcacga aaatcgtgca tggagcctgc aagctggccc tgagggccca cgttctgccc
     1681 agtattcaca ccgagattca caaagaactc aaggattctg ccgatgcggc ggccatcgaa
     1741 gtgttcagta acaacgtaaa gcaattgctt ctgtctgccc cctttggcgc aaaaacagtt
     1801 ttgggcattg acccgggtca gcgcacaggc tgcaaagtgg ttgtgatgaa cgctgaaggg
     1861 tccgttttgt gtcatgatgt tttttatctg ttgcaggaca acgacaaggc caaatcggcg
     1921 cacaaacttc tggagttggt caaaaagcat ggtgtgcaag ccgtcgctat tggtaatggc
     1981 acggctggtc gcgaaacaga agaatttgtt aaaatcattt tcaaggccca aaagattacc
     2041 ctgccagtgg tgatggtgag cgagtctggt gccagcgtgt actcggccag cgacgtggcc
     2101 agagaagaat ttccggacct cgatgtgact gtccgaggcg ccatttcgat cgcacggcga
     2161 ttgcaggacc cgctgtccga gctggtcaaa gtcgatccca aaagcattgg cgtggggcag
     2221 taccagcacg atgtgagcca gacgcaactc aagaaaaaac tcgatttcgt tgtggactca
     2281 tgcgtgaata ccgttggtgt caacgtcaat acggcatcgt atcatcttct ggcgcatgtg
     2341 tcgggcattg gccctgtgtt ggccaagacc ttggtggcgt accggcaaga acatggtttg
     2401 tttacgtcgc gaaaaagttt gctcaaagcc cctcgttttt ccaaaaaagt tttcgaacag
     2461 gccgccggtt tcttgagaat cccgcagtca aaaaatcccc tggacaatac gggcgtgcat
     2521 ccagaaaaat acagtcttct cgaaaccttt agtaaatcac tgaacaaaac catgaaggat
     2581 ttcctctttg atggggtgaa atgtctggag agctctgagg agttcaagca gcaggttggt
     2641 ctcttcacgt tcaatgacat cattgaggag ctcaaaaaac ccggtcgtga tcccagagaa
     2701 gtttacgagg tgttttcgtt cagggaagat atccatgagc tgagcgatct ctcggagggg
     2761 ttggtttgcc ccggcattgt gaccaacgtc actaatttcg gtgcctttgt ggacattggg
     2821 gtccaccagg acggtttggt gcacatatcc cagatgtcag aagcttttgt gaaagaccca
     2881 aaagacgtgg tgagtccggg tgacaaggtc gcggtcaagg ttatcagtat caatcaggac
     2941 aagcgccaga ttgggttgtc tctcaggtta tcggaggccc ccgtgaaaaa acaaatgccg
     3001 caaccgcaac aaaccaggca aatcgtgtcc tccaagggca ggacaaacgc tggacaacaa
     3061 tctttttcaa acaatccctt tgccagcctg ggatcacagc ttcgggggaa gtgacggaca
     3121 aaaaaattgt caagggggta ttgatcattg aggcaagtta aagcagttga aattcacgat
     3181 gctcgtacgc gcgtgtctat cgcttcaggc gaattgcccc gcacaaaacc ctgatgccca
     3241 ggcccagtgc aggttgtagc caccttgctc tcccgtcaca tccatcacct cacccacaaa
     3301 atacaggttt ttcaccttga gggattccat ggttttggat gataactcac gcgtgtcaac
     3361 cccacccgcc atgaccttgg ctgttttcaa atcagtcgat ttttcagaag ataatttcca
     3421 gcacgcaaag gcctgtgcga tttgttcaag ttcgtttttt gaaacaccag tcaagggttt
     3481 gtcaacaaac caatgattat tcactgtcag caccagtctc tgtggcagaa gctcggagag
     3541 aactgttttc acaaatgcct gggggcgttt tgttttttgt gtggtcaaat gctccagcag
     3601 gttcagggca ggacagaggt tgattttgag ttcatcgcct gcaggggcgt acaggctgat
     3661 gttatagacg gcgggacctg aaatgcctgt gtgtgtgaat aatagggaat ccgaataaca
     3721 ttttattcca aaggaaattt taacaggcaa agaaatgcct gccaatgttc caaacagggc
     3781 gcgatctctt ttgctcattt cccatgaggt caaagcgggg cgaggggtga tgatgtgatg
     3841 accgaactgc ttggcaattt taagccccag gtcggtggct cccaactggg gataggtgat
     3901 gcctcccgtg gctactacca atgactttgc gataaactcc ccttggttag tattcacaac
     3961 aaatttcggg gtaaactgtt gtggaagggc ctgtcctgag cgagtgaaac gacctgtcct
     4021 gagtatggtc gaaggagtcg aaggatccac ggtttgtatc ttgtaaccaa atttaaactg
     4081 tacgacgcca cactccgcca gcagggcatt aagcagttct cgcgatgatc ttttacaaaa
     4141 aagctgggtt tcgtttttgg tttcgtaagc gatgtcatgc tttttcatga gtgcaagaaa
     4201 cgcctgaggg ggaaatcgtt tgagggcaga aatgcagaat ggtctgtttt gcgaattgta
     4261 attttcagga cttatcgaaa gattcgaaaa attacagcgt cccccgcccg caatgaggat
     4321 tttggctccc ggttttggct gatgatcaat gaccaggacc ttgcgtcccc gctgactggc
     4381 ttgtgcggcg cacatcagcc ctgccgctcc ggcgcccaga atgatgacat caaattgatt
     4441 catgactgga caacatcatg aaatggggag ggagacaatg aaataatgtc ccgcagagcg
     4501 ggatcagtcc tacggctgaa aataatcaag caatcaagca atgaagcaag gtgacggtca
     4561 tatattgatt tgaagtctta agagggtttt gttagcggtt ggcgcaagag gtacttatgg
     4621 atctaaaaaa caattacaga cgaatcaccc tcaaaaccgt ggagggaact tgtatcaacg
     4681 gtaaaatcaa tattcaaaac tacaaccgtg tctccgatgt ctttgcggca agcgatgctg
     4741 agtttgttgt gctggtcgat gccaaatact gtgaggccgc tcccaaaacc atgtttgtca
     4801 acaaacagca catcgtttgg gttgagccgg aagagtgaac cgttgggaaa ttgtattgat
     4861 caacgcgcaa taattatcta ggctcaccgc ctcgtgttca aatgaggagc tcaatgctca
     4921 cttatttgca aaacaaggag tatcaaatga gctcatcaac cacgtcatcc accgccaaga
     4981 tgcccaaggg gatcccgttc atcatcggca acgaggccgc ggaacgtttc tgttactatg
     5041 gcatgcgcgc cattctggtc gtgtacatga cccaatatct gtttggcatg aacggtcagt
     5101 tggctcatgt cgacgaaccc accgccaaag gttggtatca cctgttttgt tccgccgcct
     5161 actttacgcc actcattggt gcgattatct ccgacgtgtt ttggggcaag tacaaaacca
     5221 tcatgaggtt gtccattgtt tatgccattg gctgttttgt actggcgctg gtgagcaata
     5281 cggcgggcat ggtggtgggg ctcacgctca tcgcgatcgg ttccggcggg atcaagccgg
     5341 tagtgagcgc gaatgtgggc gatcagttca ccaaagagaa tcagcacctc atcaaaaagg
     5401 tgttcagtgt attttatttc gccatcaatt ttggttcgtt tttttccacg ctccttacgc
     5461 ctcttttgct tgaatgctac gggccaaagg tcgccttttc tgttcccggt tttctcatgc
     5521 tgttggccac acttgtcttc tggataggcc gaacgcgttt tacagtcatg ccaccgagcg
     5581 gcatcaaaca atacaaggac gcgttgacga gtccagaggg caagaaagct ctcttgcatc
     5641 tgagcggctt ttttctcatc atctcatttt tctggatgct gtttgatcag tcctcggcgg
     5701 cctggatttt acaggcagat agaatggatc gggtgg
//
LOCUS       DATDUN010000435         1609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_354947, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000435 DATDUN010000000
VERSION     DATDUN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354947"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1609
                     /locus_tag="VJC18_06150"
     CDS             <1..>1609
                     /locus_tag="VJC18_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLD44999.1"
                     /translation="TVQIKIGSVSILNNLGDGVLGFATGYAHIVRSIIGGNTGNGLNV
                     RADKNLFISENAIVGNRLSAITYPAGPAEEVFVLRNILANNESTGLKDRPTTLFESNI
                     VANPRSLSLSADQVRGLAVSNNTFFSMENHADSMTVSGLLGGSGAPVEFINNAVIENP
                     RGTRLTCVPGGEYKVDYNLYYQTAAGASGACVPGAHEKVDTQPLFYIIYNDVVDIAGP
                     DFIRVEGADFIPNEFTGKFLLINQKAFFILENTVNSIKVAVPRDQAAYHLLAYGVGHN
                     TAFKITDFNFNKTSPLFNNGLNAEKQGRSLKYIHRRSFGISDIGANEIPDFTLKMSNA
                     GKAIDLTEGGDSKPVKISILNPTDKQDVTVTLNLPDRLEAEPSVLTFTGKNWELEQTV
                     NLSVIEDAEVQGAQKQDISFTLTSTNPHYNAIAVPKLSVNITDNDTAPPADPAALIVL
                     EETGDNTVVSKDGLTDTYSIKLMRRPDAAVSITPQPTASLKVSTPKLVFNLYNWNKPQ
                     TVTVSALKIPDNIGAPPINTSLAKKVQQ"
BASE COUNT          448 a          449 c          365 g          347 t
ORIGIN      
        1 aacagtccaa ataaaaattg gctccgttag catccttaac aacctgggtg atggtgtgct
       61 cggtttcgcc acaggctatg cacacatcgt tagatcaatt atcggtggca atacaggtaa
      121 cgggttgaac gtgcgtgcag acaaaaatct ttttataagt gagaatgcca tcgtagggaa
      181 tcggctctca gccatcacgt accctgctgg gccggcggaa gaagtttttg ttttgagaaa
      241 catcctcgcc aataacgaaa gcaccggcct caaggatcgg cccaccaccc tctttgagag
      301 taacatcgtg gccaatccca gaagcctgag tctctccgca gatcaggtcc gtgggctggc
      361 tgtgagcaac aacacctttt tctccatgga aaaccatgct gacagcatga ccgtttcggg
      421 tctcctggga gggtcaggtg caccggtcga atttatcaat aacgctgtga tcgaaaatcc
      481 acggggcacc agactgacct gcgtccctgg cggtgagtac aaagtcgact ataatttgta
      541 ttaccaaaca gcggctggcg cttccggggc ctgtgtcccc ggtgcacatg aaaaagtcga
      601 cacacaaccc ctgttttaca ttatctacaa tgacgtcgtc gatattgccg gtcccgattt
      661 catcagggtt gagggagccg attttattcc gaatgagttt acaggaaaat tcctgctcat
      721 aaaccagaag gcatttttta tcctggaaaa cactgttaat tccatcaagg tcgctgtccc
      781 aagagatcag gccgcttatc atctcctggc ctatggtgtg gggcacaata ccgctttcaa
      841 aataacagat tttaatttta acaagacctc tcccttattc aacaatggtc tcaatgccga
      901 aaaacaaggc cgctcgttaa agtacatcca ccgccgtagt tttggcatat ctgatatcgg
      961 ggccaacgaa atcccggact tcacgcttaa gatgagcaat gctggcaagg ccatcgacct
     1021 gacagaggga ggagacagca aaccagtcaa gatcagtatc ctgaacccaa cagataagca
     1081 ggatgtcaca gtcaccctca atctgccaga cagacttgaa gccgagccct cagtcctgac
     1141 cttcaccggt aaaaattggg agctcgaaca aacggtcaac ctatccgtga ttgaggacgc
     1201 tgaggtccag ggcgcgcaaa aacaggacat ctcgttcaca ctcaccagca caaacccaca
     1261 ctacaatgct atcgctgtcc ccaaactctc cgtcaacatt acagacaacg atacggcgcc
     1321 accagctgac cctgccgcct tgatcgtgct tgaggaaacc ggcgacaata ctgtggtgag
     1381 caaggatggt ctgacagata cgtactccat taaactgatg aggagaccag acgccgcggt
     1441 ttccatcacc ccacaaccca cagcgtcact gaaagtgagc accccaaaac tggtgttcaa
     1501 cctctacaac tggaacaagc cgcagacggt tacagtctcg gccctgaaaa tccccgacaa
     1561 catcggtgcc ccccccatca acacgtcact ggccaaaaaa gttcagcaa
//
LOCUS       DATDUN010000436         1101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_62539, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000436 DATDUN010000000
VERSION     DATDUN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62539"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VJC18_06155"
     CDS             complement(<1..997)
                     /locus_tag="VJC18_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45000.1"
                     /translation="MSNNTITRSCVFMVLFLGLMSLNSCKSGGGANPDSGDDDLSAEE
                     GGSSVYLMGSVEHLDNDADGTIDRMESYTYDANKNVTKVTYDTNLDGSAEEVAEYTYD
                     AYNSYTKFMQTVGDAAPKIYTYENTYDANHLLANVKISLEGSLFAEADYQHGVNAQNG
                     YNYEYLVTKEGQEYFEDYFLSYHQDDQYSVEGNIATCYVEVDTLYCEAKTRNTNGDCE
                     AETYYASIPLMGDDEDAIAIMNDNEVGSDPLHLEELLEVTADLSTLADNSQVTTVSYQ
                     YVYDLKNHFVVKTTDMGGDGVIDSLRYRTMTYSDGTTTTFTDADADGFPKGIDCDD"
BASE COUNT          284 a          270 c          219 g          328 t
ORIGIN      
        1 catcatcaca atcaatcccc ttcggaaaac catctgcatc ggcgtctgtg aaggttgtcg
       61 tcgtcccatc gctataagtc atggttctgt atctgagaga gtctatcaca ccatcaccgc
      121 ccatgtccgt tgtcttgacc acaaagtgat tcttcaaatc gtaaacatat tgataagaaa
      181 cagttgtcac ttgtgaatta tctgctaacg tggacaaatc tgccgtgacc tcaagcagtt
      241 cttcaagatg aaggggatca ctgcccactt cgttgtcgtt catgatcgca atcgcgtctt
      301 catcatcacc catgagtggt atgctggcat aataagtctc agcctcacaa tcaccgtttg
      361 tgtttcttgt tttggcctcg caatagagtg tatcgacctc aacataacag gtggcaatgt
      421 tcccttccac agaatactga tcgtcttgat gatagctgag aaaataatcc tcaaaatact
      481 cttgcccctc tttggtcacc agatactcgt aattgtaccc gttttgggca ttcacgccat
      541 gttgataatc ggcttcggca aagagactac cttccaggga aattttgaca ttggcaagca
      601 gatgattcgc atcgtatgtg ttctcataag tgtaaatttt tggtgcagca tcgcccactg
      661 tttgcataaa ttttgtgtag gaattgtatg catcgtacgt gtattcggca acttcttcgg
      721 ctgagccatc aaggttggtg tcgtaggtca ctttggtgac atttttgttt gcatcgtagg
      781 tgtaagactc cattctatca atcgtgccat cagcatcgtt gtccaggtgt tcgacactcc
      841 ccatcaaata aaccgatgag cctccctcct ctgcggagag atcatcatca ccactgtccg
      901 gattggctcc accacctgac ttgcaggaat tcaaagacat gaggcccaag aacaacacca
      961 tgaatacgca tgatcgggtg atagtattat tactcataga aatcctttcg ctgacctgtc
     1021 ccacacaatg acaagcaact ttcatgccta cacacgaaat tctatgttgt tgtttttatt
     1081 gcaatgtttt aaaaatactg c
//
LOCUS       DATDUN010000437         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_604499, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000437 DATDUN010000000
VERSION     DATDUN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604499"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="VJC18_06160"
     CDS             complement(<1..1530)
                     /locus_tag="VJC18_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HLD45001.1"
                     /translation="MKTQITTALVIIFLVLTVSTAAFGATLTVTKTADSLDGACNADC
                     SLREAMTAAVSGDAIVFGINPATDAGCVSETGVCTLQPLSGLPVLTQGNIIINGFSQT
                     GAVMNTADFPAAINASLKVVIDGTLAGSVSAFLVNSGHNAIKGLVIQEFAGRGVYVYG
                     GDENIIQGCFIGVDVTGELDKGNGYSGVLLGGAVRDNIIGTDGDGIADAGERNLISNN
                     ASGGIYLDDAGGNRVAGNFIGTNKTGLVDLGNTANGIWALEGSDDNIIGTDGDGVYDA
                     GEGNLVSGNVSSGIALTSYNNIVAGNYIGVTAGGNASLKNDNSGISITGNGNRVGTNG
                     DGVSDVLERNIISGNSGLSMRGIIGGGTSQDVVVAGNYIGVGADGVTDVGQDRGIQTT
                     STTSNWIMGTNGDGNGDAGEGNVISGNVYNVYLEGTSHVIAGNIVGLRADGLAAMDST
                     YGVRVFGGTGHRVGTNADGVSDSAERNILSGQTAFGVNVEGDASNTVIAGNYIGTDIT
                     GA"
     gene            complement(1713..>2175)
                     /locus_tag="VJC18_06165"
     CDS             complement(1713..>2175)
                     /locus_tag="VJC18_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLD45002.1"
                     /translation="KQLGLIDHLPQIIPVQADGSSAIVNALNDKADHVTSLVLAQTVA
                     DSISVSAPACGDWALRIVRETQGFGIRVADQEIISAIRELASLTGIFAEPAAATAYAG
                     LKKAVASQLISSDETVVLLITGTGLKDVPTAMRAVTIPESIEPKIELVVSL"
BASE COUNT          548 a          670 c          472 g          485 t
ORIGIN      
        1 ggctcccgtg atatcggttc ctatgtaatt tcccgcaatg acggtattgc tggcatcgcc
       61 ttccacgttc acaccaaaag ccgtctgccc cgacaaaata tttctctctg ctgaatcact
      121 cacaccatcg gcgttcgtcc ccacgcgatg tccggtcccg ccaaaaaccc tcacaccgta
      181 agtcgaatcc attgccgcca acccatcagc cctcaaaccc acaatattgc cggcaatcac
      241 atgggatgtt ccctcgagat acacattgta cacgttcccc gaaatgacat ttccctcacc
      301 agcatcacca ttgccatctc cgttggtccc catgatccag ttcgaggtgg tactggttgt
      361 ctgtatgcca cgatcctgcc ccacatctgt tacaccatcg gcacccacac caatgtagtt
      421 gcccgccaca accacatcct gactggtccc tccgcctatg atacccctca tgctgagacc
      481 ggaattgccc gaaatgatgt ttctctccaa cacatcactc acaccatccc cattggtccc
      541 cacgcgatta ccattgccgg tgatggatat tccgctgttg tcatttttaa gactggcgtt
      601 accgccagcc gtgacaccaa tgtaattgcc cgccacgata ttgttgtacg aggtcaaggc
      661 gatgccggag ctcacgttcc cggatactaa attaccctct cccgcgtcat aaacgccatc
      721 accatccgtg ccaatgatgt tgtcatcaga tccctcaagc gcccagatcc cattggcggt
      781 gtttcccaga tccaccaaac ctgtcttgtt ggtccctata aaatttcccg cgaccctgtt
      841 gccccccgcg tcatcgagat aaatgccccc actcgcatta ttggaaatga gattacgctc
      901 accggcatcg gcaatcccgt cgccgtctgt cccgatgata ttatcccgta cagcgccccc
      961 aagcaggacg cccgaataac cattcccctt gtccaactca cccgtcacat cgacaccaat
     1021 aaaacacccc tgaatgatat tttcgtcacc cccgtaaaca taaaccccac gaccagcgaa
     1081 ttcctgaatc acaagacctt tgatcgcgtt gtgacccgaa ttgaccaaaa aggcactgac
     1141 acttcccgcc aaagtgccat caatcacaac tttcagggac gcgttgatgg ccgctggaaa
     1201 atcagctgta ttcatgacag caccggtctg ggaaaatccg ttgatgatga tattgccctg
     1261 cgtcaggaca ggcaggccac tcagaggctg aagcgtgcac acacccgttt cgctcacaca
     1321 gccagcatcg gtcgcggggt tgatgccaaa aacgattgca tcaccgctca cagcggcagt
     1381 catggcctcg cggagagaac agtctgcatt acacgcccca tcgagtgaat cggctgtttt
     1441 ggtgaccgta agcgtcgccc caaaagcagc tgtggatact gttaaaacca gaaagatgat
     1501 gacaagggct gttgtgattt gtgtcttcaa gtgaatcgtc gtcccccttc cctctctgac
     1561 tacttcactg caaaaaacat tccacaatca gaggcaaaag gagggcaaca ggtctggaca
     1621 acacgagaca acagcatcac gattgttggt gttgggaaaa aatgatcagg tcatattaga
     1681 ccgctgggaa aaaaatgttc taaagggcac ctctaaaggg aaacaacgag ctctatcttg
     1741 ggttcaatgc tttcgggaat cgtcacagcc cgcatggctg tgggaacatc cttgagaccc
     1801 gtccctgtga tgagaagaac cactgtttcg tccgacgaaa tcagttggct ggcaacagct
     1861 tttttgagcc ccgcataggc cgtcgccgcc gcaggttccg caaaaatgcc tgtgaggctt
     1921 gccaactcac ggatggcaga aatgatctcc tgatccgcaa cacgaatccc aaatccttga
     1981 gtttcgcgaa cgatcctcag ggcccaatca ccacaagccg gtgcagacac agagatggaa
     2041 tcggctacgg tctgtgccaa cacaagagac gtcacgtgat ccgctttgtc attcaaagca
     2101 ttcacaatgg cagaggagcc gtctgcctgc acgggaataa tctgtgggag atgatcaatc
     2161 aggcccaatt gtttg
//
LOCUS       DATDUN010000438         1578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_146311, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000438 DATDUN010000000
VERSION     DATDUN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146311"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /gene="rpsT"
                     /locus_tag="VJC18_06170"
     CDS             <1..210
                     /gene="rpsT"
                     /locus_tag="VJC18_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HLD45003.1"
                     /translation="SQKHNAHNRALKAEINTWTKKVVTSSKEEAPALLKTLQSKLDKA
                     GRKGVIHHKNAAHKVSKVMRALAGK"
     gene            285..>1578
                     /locus_tag="VJC18_06175"
     CDS             285..>1578
                     /locus_tag="VJC18_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HLD45004.1"
                     /translation="MTTEPGTNALSWITLIPIFVVVLTMAISKRAVLSFFLGLLSAIF
                     CGHQTDVLTTAKRVLSLLAEKTEITHALRWSAFNNSSYLFILLSTLMIGLFINLLYNG
                     GGAGAFGGWVGRHIKNRKSAMLITFALSLVFFFDHFFHCVMTGTIMRPATDRLRISRV
                     KLAYIIDASAAAICVLVPFTVWGNEITNYLRLSGIGTRLTGDPVAIFCHSLLFCFFAW
                     LSLYLVLLVATTRRDFGPMKVCELEAQINGALSANQKEQDYAPTVFCHGPLGKPLLLD
                     FVLPLIVLLASFVVAILQANHASAGHVPQNLIKSLQQTNMARVLFWGSFSGLSFTCLY
                     CLARKLLSPKELGRVLGKGFLATLPYLAALILAWVFGSIIGQNVKTGEFLCGFLKQTI
                     AVPLLPVLVFGISLIFAFVVGSAWVTFAVMIPLLIPIVV"
BASE COUNT          327 a          447 c          362 g          442 t
ORIGIN      
        1 agtcaaaaac acaatgcgca caatcgcgcc ctcaaggcag agatcaacac ctggaccaaa
       61 aaggtcgtca cttcctcaaa agaagaggcc cccgctctcc tcaaaacctt acagagcaag
      121 ctcgataaag ctggtcgcaa aggtgtgatt catcacaaaa acgcggccca caaagtctcc
      181 aaggtcatgc gcgctttggc tggcaaatga tcagtgtcaa ctaacaaaca agctttcact
      241 tcactggatt ggcatcatgg tttgactggt ttactcgttg tattatgaca actgaacctg
      301 gaacaaacgc tctatcctgg atcaccctca tcccgatctt cgtggttgta ctgaccatgg
      361 ccatttcgaa acgcgccgta ctgtcatttt ttttgggcct tttgagcgcc attttttgtg
      421 gtcatcagac cgacgtcttg accacggcca aaagagtctt gtcgctcctg gcggaaaaaa
      481 ccgaaatcac acacgcgctc cgctggagtg ccttcaacaa cagctcctac ctgtttattc
      541 tgttgtctac cctcatgatt ggcctcttca tcaacctcct ttacaacggg gggggcgccg
      601 gcgcttttgg aggatgggtc ggacgacaca tcaaaaaccg caagtcagca atgctgatca
      661 cgtttgcctt aagtttggtt ttctttttcg accacttctt ccattgtgtg atgaccggca
      721 ccatcatgcg tcccgcgaca gaccgtcttc gcatctcgcg ggtgaagctg gcctacatca
      781 tcgatgcctc agccgcggca atctgtgtcc tcgtcccctt caccgtgtgg ggcaacgaaa
      841 tcaccaacta cctgcgcctg tccggcattg gcaccaggct cacaggcgat cccgtcgcca
      901 ttttctgtca ttcacttttg ttttgctttt tcgcctggct atccttgtat ctggttttgt
      961 tggtggccac cacacgtcgc gatttcggcc ctatgaaggt ctgcgaattg gaggcacaga
     1021 tcaatggcgc gctcagtgcc aatcaaaaag aacaggacta cgcgcccact gtgttttgtc
     1081 atgggccctt gggcaaaccg ttgttgcttg actttgttct gccactcatc gttctcctcg
     1141 cttcgtttgt tgttgctatc ctgcaggcaa atcacgcttc tgctggtcat gtcccgcaaa
     1201 atctcatcaa atcattacag caaacgaata tggccagagt cctgttttgg gggagctttt
     1261 ccggattgag tttcacatgc ttgtactgcc tcgctcgcaa gttactctct cccaaagagt
     1321 tgggccgcgt ccttgggaag ggtttcttgg ccacactgcc ctatctggcc gcgctcattt
     1381 tagcttgggt attcggttcg atcattgggc aaaacgtcaa gacgggcgaa tttctttgtg
     1441 gctttctcaa acaaaccatc gcggttccct tgttgccagt tttggtcttt ggtatctccc
     1501 tcatttttgc ctttgtggtg ggcagtgcct gggtcacctt cgccgtgatg attccccttc
     1561 ttattcccat cgtggtca
//
LOCUS       DATDUN010000439         1021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_355065, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000439 DATDUN010000000
VERSION     DATDUN010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355065"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1021
                     /locus_tag="VJC18_06180"
     CDS             <1..>1021
                     /locus_tag="VJC18_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HLD45005.1"
                     /translation="VEELAPISIETWNTHTHPDDLAKSTKLLQIHFSKKTNHYEAECR
                     MRHKNGAWVWIYDRGKVVEWDQDGTPLRMSGTHADITRRKEVEHELELAKIAAEEASL
                     AKSEFLSSMSHELRTPLNAILGFAQILELTPNEPLSAKQKDATDQILKGGEHLLNLIN
                     DVLNLARIETGKLDLKIEPVDLQNVLDECLSVASALTQNMNITITTDNFTGAIVLADR
                     VRLKQVLFNLLSNAVKYNRKNGHVNISSKATKDSWHQISISDTGRGIPTDKRDELFEP
                     FARLGAENSIIEGTGIGLTITKRLLDAMGGRIDFISEEGVGTTFCVELPLASHRANKA
                     KSSSF"
BASE COUNT          305 a          268 c          230 g          218 t
ORIGIN      
        1 ccgtggaaga actcgcgccg atctcaattg aaacatggaa cacacacacg cacccagacg
       61 acctcgccaa atccactaaa cttctacaaa ttcatttttc aaaaaaaacc aaccactacg
      121 aagctgaatg ccgaatgcgc cacaaaaatg gggcatgggt gtggatttat gatcgcggaa
      181 aagtcgttga gtgggatcaa gacggcaccc ctttgcgtat gtcaggaacc cacgccgaca
      241 tcactcggcg taaagaggta gaacacgaat tggaattggc taaaatcgcc gccgaagaag
      301 ccagtttggc caaatcagaa tttctttcat ccatgagcca cgaattgcgt acgccgctca
      361 atgccatttt gggctttgcc caaatcctcg aactcactcc gaacgagcca ttatcggcaa
      421 aacagaaaga tgcaaccgat cagattctga aaggcggtga acacctgttg aacttgatca
      481 acgacgtgct caatctagcg cgcattgaaa cgggaaaact ggatctaaaa atagagcccg
      541 tcgatctgca aaacgtactt gatgaatgcc tgtctgtcgc atcggcacta acccaaaata
      601 tgaacatcac catcacgacg gataacttta ctggagccat tgtgttggca gaccgcgtac
      661 gtctaaaaca ggtgcttttc aaccttctgt ccaatgctgt gaaatacaat cggaaaaatg
      721 gccacgtcaa catcagcagc aaggccacta aggatagctg gcatcagatt tccatttcag
      781 acacagggcg gggcattcct accgataagc gcgatgaact gtttgaacct tttgcgcgct
      841 taggcgccga aaattccatt attgaaggca caggcatcgg cctgacgatc accaaacgcc
      901 tactcgatgc catgggggga cgtatcgatt tcataagcga agaaggcgtg ggaacgacat
      961 tctgtgtcga actgccgctg gcatctcacc gcgccaacaa agccaaatcc tcttcatttt
     1021 t
//
LOCUS       DATDUN010000440         1587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_311311, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000440 DATDUN010000000
VERSION     DATDUN010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311311"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VJC18_06185"
     CDS             <1..430
                     /locus_tag="VJC18_06185"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HLD45006.1"
                     /translation="PSQHQTTNNQQDVLLFSAQSNIEAKMIVTLLEAQGIPCRQISRG
                     AGGYLNIALGSNMFGEDLYVTADMFDQAKKILQENLATMPEANLHDDSAQDQSLSPVV
                     PDQKQQKRARLILWCLFGIPFVLIVILMLLVSFLRIYHSF"
     gene            complement(634..>1587)
                     /locus_tag="VJC18_06190"
     CDS             complement(634..>1587)
                     /locus_tag="VJC18_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45007.1"
                     /translation="EDVIRRMQFGTEGAVPRQCSKISRAVTVGGEKVARILAGREQRT
                     PYLNGYILCFALTSAGRECDLGEVMSAVGLRVFGEPFVEGSDGFGLSVAGARILVQGG
                     ELEPGWYHVQIKPSLPFENGPAMFAHELAFENLFGDAGHVVSLKSKLPAETDQPMTAK
                     PVTLLVREETYNPFDVRLTTLAREREAEAGLLALGDGSHRFHLLWQGRYPLMLTIVEG
                     MVNGGGADVSIEVRVPQSMHNELVEEAVRDYLTLLLREATEVLPERTDLVTRNLVPYQ
                     SMPAVYRLDLGKGRGSSRVQRWQRMEKIASVIRGYERFGGR"
BASE COUNT          422 a          455 c          321 g          389 t
ORIGIN      
        1 tccctcgcaa catcaaacca caaacaacca gcaagacgtc ctgctattct ccgcgcaaag
       61 caatattgag gccaagatga ttgtaaccct gcttgaggca caggggattc cgtgtcgtca
      121 gatttcgcga ggggctggtg gctacctcaa catcgcctta ggctcaaaca tgtttgggga
      181 ggacctttat gtgaccgccg acatgttcga tcaggccaag aagattctac aagaaaacct
      241 ggctacaatg cccgaagcaa acctccacga cgacagcgcc caagaccaaa gtctctctcc
      301 cgttgtccct gaccaaaaac aacaaaaacg cgcaagattg attttatggt gcttgttcgg
      361 aatcccattc gttttgatcg tgatcttgat gctcctagtc tcttttctaa gaatatatca
      421 ttcgttctaa caggcactct caggaatttc tgcattctaa aaagatatca gtcgccttgg
      481 gtgaggtggg ccgactgagc ttgtgagtgt tgggaaagtt cagattgaca tgtctccgaa
      541 ataaacttga aataaagtta ttccgttgag tcaacaacgt cccctgtccg ccgaaaaaat
      601 agaaacagag agattttcga ttcgaatagg cttttatcga cctccaaacc tctcatagcc
      661 cctgatgacg gatgcaattt tttccatcct ctgccatctt tgaactcgag atgaacccct
      721 gcctttcccc aaatcaagtc gataaactgc cggcattgat tggtaaggca ccaaatttct
      781 ggtcacaaga tccgttcgct ctggcaacac ttctgtggct tctcttagaa gcagtgtcaa
      841 atagtcgcgc actgcctcct ccaccaattc attatgcatc gattgaggca ccctgacttc
      901 tatggacaca tctgcgccac ccccattcac catcccctcg acaatcgtga gcatcagggg
      961 atatctgccc tgccataaca ggtgaaatct gtgtgagcca tcacccagtg ccagcaaacc
     1021 agcctccgcc tccctttcac gcgctagtgt cgtcaacctc acatcaaaag gattataagt
     1081 ttcttcgcga acgagtaagg taacaggctt tgctgtcatt ggctgatcag tctctgctgg
     1141 taatttcgat ttcaaagaca caacgtgccc agcatcgcca aacaaattct caaaggccaa
     1201 ctcatgcgca aacatcgccg gaccgttttc aaagggcagg ctgggtttga tctgaacatg
     1261 ataccaacct ggttcaagtt ctcctccttg caccaaaatt ctggccccag caacagacaa
     1321 cccaaaccca tctgaccctt caacaaatgg ctctccaaat accctgagac ccactgccga
     1381 cataacttcg cccagatcgc attccctgcc cgccgatgtc agggcaaagc ataagatata
     1441 accgttcagg taaggagttc tttgttccct gcctgcaaga atacgggcca ctttctctcc
     1501 tccaacagta acagcgcggc tgattttgga gcattgacga ggcacggccc cctcagttcc
     1561 aaactgcatt ctgcgaataa catcctc
//
LOCUS       DATDUN010000441         1592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_271013, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000441 DATDUN010000000
VERSION     DATDUN010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271013"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VJC18_06195"
     CDS             complement(<1..278)
                     /locus_tag="VJC18_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HLD45008.1"
                     /translation="MTLCAQKIETADYKVADMSLAEFGRKEIEIAQSEMPGLMSLRKK
                     YAEEKPLKGARIMGSLHMTIQTAVLIETLVDLGAEVRWASCNIFSTQD"
     gene            complement(294..1070)
                     /locus_tag="VJC18_06200"
     CDS             complement(294..1070)
                     /locus_tag="VJC18_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD45009.1"
                     /translation="MHRTITFIFLLLLFFSSLLSFAQDQPSIDPAFEAGKTAYFANDY
                     AQAVVQFEKVLRDHPDNGYAQYNLGNAFFKLGELGKARLYYEKAVLRLPRFQDLQINS
                     DLLKQKLTDAVEKSFGDYLSGTFYFWIGYLTMSEGRILVILLSIGLWGYCVFRILRRQ
                     QLVVTSTAIVLLIFVYLSLGCFIKYHLEKPLAYGVILPKSSDVRASYQEKDQVLFTLH
                     EGAKVRLIDHQDFGDAEKWVKISLPQGQKGWVKAQEVGEI"
     gene            complement(1266..>1592)
                     /locus_tag="VJC18_06205"
     CDS             complement(1266..>1592)
                     /locus_tag="VJC18_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45010.1"
                     /translation="NAYRVLSQTLSRRGAGADDVLDAVQVFMSHVFGVTGGALTAGEM
                     KDHCESHGVIPSVADALEKIIQELEAARYGFDKQTTVEQASKELLVVLKKIDRDAHSS
                     KLKAES"
BASE COUNT          424 a          420 c          323 g          425 t
ORIGIN      
        1 tgatcttgcg tggaaaaaat attgcaactg gcccagcgca cctcggcccc taaatcgacg
       61 agagtttcga tcagaacagc cgtctgaatg gtcatgtgca aagaccccat gatccgtgca
      121 cctttgagag gtttctcctc cgcgtatttt ttacggaggg acatgagtcc gggcatttcg
      181 gactgagcaa tctcgatttc cttgcgacca aattcagcca gactcatatc ggcgactttg
      241 taatctgctg tttcaatttt ttgtgcacat aaggtcatat tattttcctt tctctatatc
      301 tcccccacct cctgcgcctt cacccaacct ttttgcccct ggggcagact gatcttaacc
      361 catttttccg cgtccccaaa atcctggtgg tcaatcagtc gtaccttggc cccttcgtgg
      421 agagtaaaca agacctggtc tttttcctga taactggccc tgacatcact gctcttgggc
      481 aaaatcactc cgtaggcaag tggtttttcg aggtggtatt taataaaaca tcccaaagaa
      541 agatagacaa aaatcaataa aacaatggct gtgcttgtca cgacaagctg ttgccgtctc
      601 aaaatcctga aaacgcaata accccaaagg ccaatcgata acaagatcac cagaatcctg
      661 ccttccgaca tcgtcagata accgatccaa aaataaaagg tcccactcag ataatcccca
      721 aaggattttt caaccgcatc ggtcaatttt tgcttgagca aatcactgtt gatttgcaaa
      781 tcctgaaaac ggggcagacg cagaacagcc ttttcatagt acagcctggc ctttcccaat
      841 tcccccaatt taaaaaacgc gttacccaga ttgtattggg catacccgtt gtctggatga
      901 tcccgcaaaa ctttttcgaa ctgaacaaca gcctgggcat agtcattggc aaaataggct
      961 gttttgcctg cttcaaaagc ggggtcaatc gagggctgat cctgtgcgaa tgacaacagg
     1021 ctggaaaaaa ataggagaag aagaaaaata aatgttatgg ttctatgcat aaatacgtgg
     1081 gtcgacctaa aaaacctatg aatagcgaac atcgaacaag gaacaaggaa ttacgaaatg
     1141 attgtcattc cagcgaatgc tggaatccat gtttacgata attctggatc cctgcattcg
     1201 cagggatgac acatttcgac attcgacatt ccttgttcgt tattcgatat tcttgccccc
     1261 ccctttcagc tttcagcttt gagctttgag ctgtgtgcat ccctatctat cttcttgagc
     1321 actaccagaa gttctttgct cgcttgttcg actgtggttt gtttgtcgaa cccgtatctc
     1381 gccgcttcca gttcctgaat gattttttcc aaagcatctg ccactgaggg gatcactccg
     1441 tgagactcac aatgatcttt catttcccca gctgtcagtg ccccgcccgt cacgccaaaa
     1501 acatgcgaca tgaaaacctg cacggcatcc agcacgtcat cggctcccgc gcccctgcgg
     1561 gacagcgtct gactcaaaac acggtaggca tt
//
LOCUS       DATDUN010000442         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_395188, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000442 DATDUN010000000
VERSION     DATDUN010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395188"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VJC18_06210"
     CDS             complement(<1..1262)
                     /locus_tag="VJC18_06210"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HLD45011.1"
                     /translation="MKIKTCLFLLICLVSSWGAFADPCEVVNNHDEGDGSGYNSSSLR
                     YIVDDFINGLTNAHCDSLGSEDDADFDQTIIFNSALDIELNNSAGGIVFDVETAAGQL
                     IVGSSSSTLSKIDGTLLGAGESPFQCSSGTADSAVSLRNLLIITKDTNEADFYLNNLC
                     LIATENVYVCQEADYDDSVSPGTTGWCGGDDDDDDDDDGFPDFPHDLWPPILMRFYAT
                     VYADADGDAYGDAGVSLQVEKHLRMYDSDGDGIKDKFGWQYTVPAGFVEENTDCDDTT
                     AARFPGNPEICDGLDNDCNFIVDDGLDCTDDDTIYYPDDDGDAYGDPVGPTVTDTTPP
                     DGYVEDHTDCNDANAAIYPGAAEVCDGVDNDCDGQVDEGFDVDGDGYTTCEGDCDDGD
                     IAVHPEGTEICEDGIDQDCNGSDEICAE"
     gene            complement(1285..>2461)
                     /locus_tag="VJC18_06215"
     CDS             complement(1285..>2461)
                     /locus_tag="VJC18_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLD45012.1"
                     /translation="VAKNLHLGLQGGGTFNIFYDHDGAQYYDQGEYNGTNVFGSSYKH
                     GSLYAGPVLALGQGGAVLVNPYLSIPFYFNDQNTVRADLPGTARIVGTQSPVIAGVSM
                     TWRPKITDAFRLRVTGSTDPHFVLKTQSNSSETTPETIGDSKLNDIYRDPNSLIARLG
                     VDFVWINRAAPQPIPVPQPEVIPELVVAPSLVVPESKPVIIPPLAVVPPPVEVPPLVI
                     VTETQIKITEQIMFETNKAVIDESSYPLLDAIVQAVRENPDIALIRIEGHTSTTGSNL
                     LNWKLSSARAQAVQQALTTRLGTTESARFYAVGYGEEELEVPTADNVEEPANRRVEFA
                     IMLKKTAPGDVLVIANDDEGKARYYQTDLIPPTGAPPSTVILERKIIADRKSALERK"
BASE COUNT          654 a          641 c          537 g          629 t
ORIGIN      
        1 tcttccgcac agatttcatc ggaaccgtta cagtcctgat caataccatc ttcgcaaatt
       61 tctgttcctt ccggatgaac agcaatgtct ccatcatcac aatcgccttc gcacgtggtg
      121 taaccatcac catcaacatc aaagccttca tcaacctgac catcacagtc attgtcaacc
      181 ccgtcacaca cttctgctgc tcccggatat atagccgcat tcgcgtcgtt gcagtcggta
      241 tgatcttcaa cgtagccatc aggaggagtt gtgtccgtga ccgtggggcc gacggggtcg
      301 ccgtaagcat caccatcgtc atcgggatag taaatggtgt cgtcatcggt acaatcaaga
      361 ccgtcatcca ctataaaatt acagtcgtta tcaaggccgt cacaaatttc tggattaccg
      421 gggaacctgg cggctgttgt gtcatcgcag tctgtgtttt cttccacaaa acccgcagga
      481 accgtgtatt gccaaccaaa tttgtctttg ataccatcgc catcggaatc atacatgcgc
      541 aggtgttttt cgacttgtag actcacaccc gcgtcgccgt atgcatcgcc gtccgcatcg
      601 gcatacacgg ttgcataaaa gcgcatcaga attgggggcc agagatcgtg aggaaaatcg
      661 ggaaagccat catcatcatc atcatcatca tcatcaccac cgcaccaacc tgttgtgccg
      721 ggagaaacgc tgtcatcgta atcggcctct tgacagacgt acacattttc tgtggcaatc
      781 aaacacagat tgtttaagta aaaatccgct tcattggtgt ctttcgtaat gatcagaaga
      841 ttccgaagac tcacggcaga atctgccgtt ccggaactgc attgaaatgg gctttcaccc
      901 gcaccaagca atgtaccatc aatttttgac aacgtgctgc ttgatgaacc cacaatcagc
      961 tggccagccg ccgtttccac atcaaagaca attccacctg cactgttgtt gagttcgata
     1021 tccagagcac tgttgaaaat aattgtttga tcgaagtcgg catcgtcctc tgaaccaagt
     1081 gaatcacaat gggcatttgt gaggccatta ataaagtcat cgacaatata acgtaaggag
     1141 gatgaattat agccgctgcc atccccctcg tcgtgattgt tcacaacttc acacggatcc
     1201 gcaaaagcgc cccaggaaga aaccagacaa atcaacagaa ataaacatgt tttgattttc
     1261 ataataggac ctcgttcttg tttattattt tcgttccaaa gcgctcttgc gatcggcaat
     1321 gatttttctt tctagaatta ccgtgctggg cggcgcccct gttggaggaa tcaaatcagt
     1381 ctgataatat cgcgccttcc cctcatcatc attagcgatc accaagacgt ctcccggggc
     1441 agtttttttc aacatgatgg caaactcaac acgccgattc gctggctcct caacattgtc
     1501 agccgttggc acttcaagct cttcttcacc atagccaaca gcgtaaaacc gtgcagactc
     1561 agtggtacca agccttgtgg tcagggcttg ttgaacagcc tgagcccgcg cactggacag
     1621 tttccaattg agcaggtttg accctgtggt gcttgtgtgt ccttcaattc tgatcaaggc
     1681 gatatctgga ttttctcgaa cagcctgcac tatagcatcg agcaatgggt aagagctctc
     1741 gtcaatcacc gctttattcg tttcaaacat aatctgttct gtgatcttaa tttgtgtttc
     1801 cgtcacaata acaagaggtg gaacttcaac cggcggagga acaaccgcta gcggtggaat
     1861 aatgacaggc ttagattcgg gcacaaccaa agaaggcgca accacaagct cagggataac
     1921 ctctggctga gggactggaa ttggctgagg tgccgctctg tttatccaaa caaaatcaac
     1981 gcccaaacga gcgatcaaag aattgggatc gcgataaatg tcatttaatt tcgaatcacc
     2041 gattgtttca ggagtcgttt ctgaggaatt tgattgagtt ttcaaaacaa aatgcggatc
     2101 cgttgaacct gtgacgcgta atctgaaagc gtccgtaatt tttggtcgcc aggtcatgct
     2161 gacaccagct atcacaggac tttgtgtgcc aacaatacgg gctgttccag gcaggtcagc
     2221 acgaaccgtg ttctgatcat tgaagtaaaa cggaatgctt aagtacggat tgaccagaac
     2281 agcaccaccc tgaccgagag ccaggaccgg tcctgcataa agactgccgt gcttgtaaga
     2341 cgatccaaaa acgtttgtcc cattgtattc accttgatcg tagtactgcg cgccatcgtg
     2401 atcgtaaaag atattgaacg tgcccccccc ctgcaaaccc aaatgcaaat tcttggcaac
     2461 a
//
LOCUS       DATDUN010000443         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_188373, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000443 DATDUN010000000
VERSION     DATDUN010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188373"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2025)
                     /locus_tag="VJC18_06220"
     CDS             complement(<1..>2025)
                     /locus_tag="VJC18_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HLD45013.1"
                     /translation="GAGFTWQGNSQRNRLTAWSNDPVLDPPCEAIYIRDEDTDAYWTP
                     TASPIREKTAYRAKHGAGYSLFEHNSHGLNQELTVFVPVNDQGGEPLKLQRLKLVNDS
                     GRPRKISLTYYVEWTLGENREASQMHVVTDWDDELQMLIASNSYNPDYPKCVAFAALN
                     GPVESYTGDRTAFLGRNRSLKNPRALERMRLPQRTGAGLDPCAALQVIVQLAPGEQTE
                     LVCLLGQTPSVASAHTLVLNYRRKHAFEKSFTQTKNWWDQNLNTIQVQTPELSTNLLI
                     NRWLLYQTLSCRIWGRSAFYQSGGAFGFRDQLQDVMALLYMQPKLARQHILLSASHQF
                     NEGDVQHWWHPPIGSGIRSRISDDLLWLPYVVWQYVQVTGDRGILNESVPFISAPELE
                     SQQLELFLLPNISSESATLFEHCQRAVTRAQKYGVHGLPLMGTGDWNDGMNRVGAQGK
                     GESVWLAWFMAEVLRRLATMAELVNKTKLAQTYLQERETLIQNVDDFAWDGEWYLRAT
                     FDDGSLLGCHNNTEAKIDSLPQSWAWLCGKTGNDRAQMALESVWKHLVKEDGRLTLLF
                     TPPFDESIPSPGYIKGYPPGVRENGGQYTHAALWFAMALAKSGDGDRAAKLLRFVNPI
                     ECARTPVDVWRYNVEPYVVAADVYALKDRVGMGGWSWYTGSASWMYRIWV"
BASE COUNT          502 a          548 c          482 g          493 t
ORIGIN      
        1 ccacccaaat tctgtacatc caagaagcgg aacccgtgta ccaagaccag ccgcccatcc
       61 caacacgatc cttaagcgca tacacatcgg cggccaccac ataaggttcc acattgtaac
      121 gccaaacatc cacaggtgtc cgagcacatt caatgggatt aacgaaacgc aacaattttg
      181 ccgcgcgatc gccatcacca cttttggcca gagccatcgc aaaccacaaa gccgcgtgtg
      241 tgtactggcc accgttttct ctaacccccg ggggataacc cttgatgtac ccaggtgaag
      301 ggatcgattc atcaaacggt ggcgtaaaaa ggagtgtgag tcttccatct tctttgacca
      361 gatgtttcca aacagattcc aaagccattt gcgcgcgatc attaccggtt ttgccacaaa
      421 gccaggccca agactgggga agcgaatcga tttttgcctc tgtgttattg tggcatccca
      481 aaagcgagcc gtcatcaaac gtagccctga gataccattc gccgtcccag gcaaaatcgt
      541 ctacattttg aatgagagtt tctctttctt gcagatacgt ttgtgcaagt tttgttttgt
      601 tgaccagctc tgccattgtg gccaggcgcc tcagcacctc cgccataaac caggccagcc
      661 aaacgctttc acctttcccc tgagctccca ccctgttcat cccgtcattc cagtctcccg
      721 tgcccatcag cggcaaaccg tgaaccccgt atttttgtgc ccgcgtcaca gcgcgttgac
      781 aatgttcaaa gagagtcgcg ctctccgatg aaatattggg cagtaaaaaa agttccagtt
      841 gctgactttc caattcgggt gccgagatga aaggaacgga ttcgttgaga atgccccgat
      901 cacctgtgac ctgaacgtat tgccaaacaa cgtaaggtaa ccacaaaaga tcgtctgaaa
      961 tgcgtgatct gattccggaa cctatagggg ggtgccacca gtgttgcaca tcaccttcgt
     1021 tgaactgatg actggcagaa agcaaaatgt gttgacgggc cagttttggt tgcatgtaca
     1081 gcagtgccat cacgtcctgc aattgatcgc gaaacccaaa agccccgccc gattggtaaa
     1141 aagccgaacg gccccaaatg cgacacgaaa gtgtttgata caacagccag cggttgatca
     1201 aaagatttgt ggacaattca ggtgtttgaa cttgaatggt gtttaagttc tgatcccacc
     1261 aattttttgt ctgagtgaag gatttttcaa aagcgtgctt cctgcgataa tttaatacca
     1321 acgtgtgtgc tgaggcaaca gaaggtgttt ggcccagcaa acaaaccagc tcggtctgtt
     1381 cacccggtgc gagctgcacg atgacctgca atgccgcaca gggatccagt cctgctccgg
     1441 ttctttgggg aagacgcatg cgctcaagag cccggggatt ttttaaagaa cggtttcttc
     1501 ccagaaaagc ggtgcgatca ccggtgtaag attcaacggg accgttcaag gccgcaaagg
     1561 ccacacactt cgggtaatcg ggattgtagc tgttgctggc gatcaacatc tgaagttcat
     1621 catcccaatc agtcaccaca tgcatttgcg atgcctctct gttttctccc agggtccact
     1681 ccacatagta tgtgagtgag attttgcgtg gacgaccaga atcgtttacc agttttaacc
     1741 tttgcagttt caggggttcg ccgccttgat cattcaccgg cacaaaaacc gtgagttctt
     1801 gattcagccc gtgactgttg tgttcaaaca acgaataacc cgccccgtgt tttgcccggt
     1861 aggcggtttt ttcacgaatc ggtgatgctg tgggtgtcca ataagcatct gtgtcttcgt
     1921 cgcgaatgta aattgcctca cacgggggat cgagaacagg atcgttggac cacgccgtta
     1981 gcctgttgcg ctgactgttg ccctgccacg taaaaccggc acccg
//
LOCUS       DATDUN010000444         1010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_437805, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000444 DATDUN010000000
VERSION     DATDUN010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437805"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /gene="pth"
                     /locus_tag="VJC18_06225"
     CDS             <1..494
                     /gene="pth"
                     /locus_tag="VJC18_06225"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLD45014.1"
                     /translation="NVGFMALDHLWADHKDDFHLTAWTNHKKFSAEISEGIVNQEKII
                     LIKPNTFMNESGRAASAISHYYQVAPLDIIVVHDDVDLIFGSYKIQKNISSAGHNGIK
                     SIIQHLKTEQFHRVRIGIAKTDKEKQGDTAKFVLNQFSLFERLKLNKLLTQITEEIIT
                     LIK"
BASE COUNT          319 a          269 c          172 g          250 t
ORIGIN      
        1 acaacgtcgg tttcatggct ctcgaccatt tgtgggccga ccacaaagat gattttcatt
       61 taaccgcgtg gaccaaccac aaaaaatttt ccgccgagat ctctgaaggc atcgtcaatc
      121 aagaaaaaat catcctcatc aaaccgaata ccttcatgaa cgaatcaggt cgagcagcat
      181 cagctatcag tcactactat caagtcgccc ccttggatat aattgttgtt cacgatgacg
      241 ttgatttgat ttttgggtca tacaaaatcc aaaaaaacat ctccagcgcc ggccataatg
      301 gcatcaaatc gattattcaa catctaaaaa ccgaacaatt tcatcgcgtc cgaatcggga
      361 tagccaaaac tgacaaagag aaacaaggcg acaccgctaa atttgtcctt aatcaatttt
      421 ccttgtttga acgactcaaa ctgaacaaac tcctgactca aatcactgaa gaaatcatta
      481 ctctcatcaa ataaaaacac gagaccttgg tctcgtgttt ttattcaaaa aacctctctc
      541 gaggtttcac caaaaattct catcaatgct catcagcgca aattgcttaa gaccagctca
      601 agcaacttaa cttctggggc aggaagctag cacaggaggg gggtactggc ttcaccccgc
      661 tgataagcat tgatcaaagt tctttcattc tcaccttaac aaattctgtg atcatgtcaa
      721 gcccaaagag aacacttttt gtgttcaatt aaaaaaggag cccatttgct ccttcatatc
      781 agtaacaatc aaccacaaag tcaaacaata aagtccccct gaatgctgaa tcatcaccac
      841 acgaaccttg accccgggta atacatcttg ccacggaacg tcaagtaact atcgtctgtc
      901 tcgtgcatat gcacccggac gacattgtta ccccgagccc gaagctttgc cggtctcggc
      961 ttccgcgcca gtcggacaaa attcatatat tccaacccaa tcaccggccg
//
LOCUS       DATDUN010000445         2480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_395289, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000445 DATDUN010000000
VERSION     DATDUN010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395289"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VJC18_06230"
     CDS             <1..1334
                     /locus_tag="VJC18_06230"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HLD45015.1"
                     /translation="LVIFAIGLTLSTIAKQQKIRYIGSIILGFGLIFYGMHLMTSSAE
                     PLKNSEVAASIFAFLKDHPFLNLAFAAAFAGFVHASAVTIGFAIALSFSGALPFEAAI
                     PIVLGANVGTCITAALASIGMSIQGKRVAVAHILIKLLGVILLMPILTYVASWINWIN
                     DLLTQYTSLVLPLSGKIVLTHILFNLFLAIIFLPCIPLLVWMVQKIVPTTKNLMEKFG
                     PKYLDKSALDTPPIAFANARHEILRISKIAREMYTEIFDMFRSDENIEKAITVTKEKD
                     DHIDALEKAVRFYLSKLSQQQLTEGQGTMQTALTSIAGEFENIGDEISKDVIALARKK
                     QTKMSRFSDEGWGELQRLHKCVLANFDLTIGMLMQPHEDIARKVTRNNDEIKEMEQDL
                     RSAHLQRLNENRIESFETSTIHLDLLSQFRAINYRLVKTVYMLKELSQLKD"
     gene            1387..2025
                     /locus_tag="VJC18_06235"
     CDS             1387..2025
                     /locus_tag="VJC18_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HLD45016.1"
                     /translation="MGLLELPQGLFPILDETHTNGLSTFEIIEAFVDAEVDPFIVFCH
                     SLSPTEYADYIQAISLLKYEMDFDYLLHHHLDRMPVSGAAGIHLTYKSVSIKQARRDL
                     GPEALVGYSARSCDEALKAQDEGADYILLGSIFQSNHFHQDYPVIGLEELNKTCRRLT
                     VPVYASGGIQPSNLIMVKDSGAAGFTALDAVYIDGEIDHNISLLNILWEEMD"
     gene            complement(2094..>2480)
                     /gene="amrA"
                     /locus_tag="VJC18_06240"
     CDS             complement(2094..>2480)
                     /gene="amrA"
                     /locus_tag="VJC18_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HLD45017.1"
                     /translation="GCIGVLEAHRALIEDVAENAYAAAFRDSRFSPLTPSELDQIEVS
                     VSILEPAQPMKFTSEEDLINQIQPGIDGLILEEGWHRGTFLPAVWDSVKTAREFLNHL
                     KVKAGLPMDYWSETIKIKRYTTEMVE"
BASE COUNT          611 a          708 c          563 g          598 t
ORIGIN      
        1 ccttggtgat ttttgcgatc ggcctgaccc tgtccaccat cgccaagcaa caaaaaatcc
       61 gttacattgg ttccatcatt ctgggttttg gtctcatttt ttacggcatg cacctcatga
      121 ccagttccgc ggagcccctc aagaacagcg aggtggccgc cagcatcttc gcatttttga
      181 aggatcaccc cttcctgaac ctggcctttg ccgccgcgtt tgccgggttt gtccatgcct
      241 cggcggtcac cattggtttt gctattgccc tctcgttttc cggtgccctt ccgtttgaag
      301 ccgcgatccc gattgtgttg ggggccaacg tggggacctg catcaccgcc gctctcgcca
      361 gcattggcat gagcatacag ggcaagcggg ttgctgtggc ccacatcctg atcaaactgc
      421 tgggcgtgat cctcctcatg cccatcctga cctatgtggc cagttggatc aactggatca
      481 atgatttgtt gacgcagtac acgtcacttg tccttcccct cagcggcaaa atcgtgctga
      541 cccacatttt gttcaacctc ttcctcgcca tcattttcct accctgcatc cccctccttg
      601 tttggatggt gcaaaaaatt gtccccacga ccaaaaacct gatggaaaaa ttcggcccca
      661 agtatctgga caaatccgca ctggacacgc ctcccatcgc ctttgccaac gccagacatg
      721 aaattctgag gatttcaaaa attgcgcgcg aaatgtatac cgaaattttt gacatgttcc
      781 ggtccgatga aaacatcgaa aaggctatca cggtaaccaa agaaaaagac gatcacattg
      841 atgctctcga aaaagccgtt cgtttttatc tgtccaaact ctcacaacaa caactgaccg
      901 agggccaagg caccatgcaa accgcattga cctccattgc gggagagttt gaaaatatcg
      961 gagacgaaat ttccaaggac gtcattgcgc tagccagaaa aaaacagacc aaaatgtcgc
     1021 ggttttctga cgagggatgg ggagagttgc aacgcctgca caaatgtgtc ttagccaact
     1081 ttgatctgac catcggcatg ctcatgcaac cacacgaaga catcgcccgc aaggtcacac
     1141 gtaacaacga tgagatcaag gaaatggaac aggacctgcg ttcggctcat ttgcagagac
     1201 tcaacgaaaa ccgcattgag tcgtttgaga ccagcacgat tcatctggat cttttgagcc
     1261 agttccgcgc catcaattac cggttggtaa aaacggtgta catgctcaaa gagctgtccc
     1321 agttgaaaga ttgataatgt tgtcacaacc gttgcctaaa ccccaaaaat ataatacggc
     1381 aatcgcatgg gtctactcga actaccacag ggactttttc cgatactgga cgaaacccac
     1441 accaatgggc tgtccacgtt cgaaatcatc gaagccttcg tcgatgccga ggtggatccg
     1501 ttcatcgtgt tctgtcacag cctgtcgcca acagaatacg ccgactacat tcaggccatt
     1561 tctctgctca aatacgaaat ggattttgac tacctcctgc accaccatct ggaccgcatg
     1621 cccgtttccg gcgcggcggg tattcacctc acctacaaat ctgtcagcat caaacaggca
     1681 cgccgcgacc tcggcccgga ggctcttgtg ggctattccg ctcgatcctg cgacgaggcg
     1741 ctcaaggcgc aggacgaagg ggcagactac attttgctgg gatccatttt tcagagcaat
     1801 cactttcacc aggactatcc cgtcataggg cttgaagagc tcaataaaac ctgtcgtcga
     1861 ctgacagtgc ccgtgtacgc ctcgggcggt attcagccca gcaatctcat catggtcaag
     1921 gacagcgggg ccgcggggtt cacggccctc gacgccgtgt acatcgacgg cgaaatcgat
     1981 cacaacattt ctttgctgaa tattttgtgg gaagagatgg actgaaaaaa gtctaatggc
     2041 caacaaccaa caactttcgt ccatcgtccc tcgtcctttc gtccttcgta ttttcactcc
     2101 accatctccg tcgtgtacct tttgatttta atggtttcag accagtagtc catcggcagg
     2161 ccggccttga ctttgaggtg attcaaaaat tccctggccg ttttgacaga atcccacacc
     2221 gcaggcaaaa aggtcccgcg atgccagcct tcttccaaaa tcaaaccatc gatccctggc
     2281 tgaatctggt tgatcaaatc ctcctctgac gtaaacttca tgggctgtgc tggttcaaga
     2341 atggaaacgg aaacttcaat ctgatccagt tcagatggtg tgagcggcga aaagcgagaa
     2401 tccctgaacg ccgcggcata ggcattttcg gccacatcct cgattaaggc tctgtgtgct
     2461 tccaataccc caatgcaacc
//
LOCUS       DATDUN010000446         1090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_605191, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000446 DATDUN010000000
VERSION     DATDUN010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605191"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VJC18_06245"
     CDS             complement(<1..237)
                     /locus_tag="VJC18_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HLD45018.1"
                     /translation="MALILKNGVVVTAADTCKADVKIEGEIITHIGRNLPSAIGDQTI
                     DAIGKYIFPGGVDAHTHFELPFMGTYSADDFHTGT"
     gene            complement(350..649)
                     /locus_tag="VJC18_06250"
     CDS             complement(350..649)
                     /locus_tag="VJC18_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLD45019.1"
                     /translation="MERSIKIEYSKTAATIIRHLPPSAKGPVRHLIEQLSTQPCLGKP
                     LRDELEGYRSHRYNRYRVIYCYHEDKKLIEIIFAGQRADVYSLFSRYLKTQKKKQ"
     gene            complement(634..906)
                     /locus_tag="VJC18_06255"
     CDS             complement(634..906)
                     /locus_tag="VJC18_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HLD45020.1"
                     /translation="MGKYDKILPITDVKKHLLSYVKDLCELNESIAITRNGYPTAVLL
                     SVDEYEGLLETIEILSDAKLMKVINKSRKDMETGRTLSHADVWNDQ"
BASE COUNT          275 a          242 c          240 g          333 t
ORIGIN      
        1 tgtaccggta tgaaaatcgt cggcactgta ggttcccata aatgggagtt caaagtgagt
       61 atgcgcatcg accccgccgg gaaaaatgta tttgcctatt gcgtctatgg tttgatcgcc
      121 aattgccgaa ggcaagttcc gtccgatgtg tgtgatgatt tcgccttcta ttttgacatc
      181 ggccttgcag gtatcggcag ccgtcaccac aaccccgttt tttagaatca gagccatatc
      241 cccctcctca caattcgtcc gacatcttct ttatcaagtt tgattggttt atcaagaaag
      301 taaacgattt gttcaatgtg attgatttgt gttgcaaaat gaaaacagat cactgtttct
      361 ttttttgtgt cttcaaataa cggctaaata agctgtaaac atcagcgcgt tgacccgcaa
      421 aaataatttc aatcaacttc ttgtcttcgt gataacaata aatcacacgg tagcggttat
      481 agcggtgaga acgataacct tccaactcat cgcgcaaagg cttgccaaga cagggttgcg
      541 tggacaattg ttcaatcaaa tgccgaacag gtcccttggc cgaggggggc agatgacgaa
      601 tgattgtagc ggcggtcttt gaatattcga tttttattga tcgttccata catcagcgtg
      661 tgaaagggtt ctgcctgtct ccatatcttt gcgggattta ttgatcacct tcatgagttt
      721 tgcatcgctg agaatttcga tggtttccaa cagtccctcg tattcgtcga cactgagcaa
      781 tacggcagtg gggtacccgt tacgtgtgat ggcaatggac tcattcaatt cacacagatc
      841 cttcacgtaa gacagcaagt gttttttgac atctgtgatt ggcaggattt tatcgtattt
      901 tcccataatg acctctttaa tgactataat tatggtaatt catggcctcc cataagtcaa
      961 ctgttatcat caggtcatcg ggtcatcatc tggtaatctc acggcaggag cttgatcata
     1021 ttctcatacg tctcaggtct gcgatcgcgg tagaattgcc agaggtcgcg cacttctcga
     1081 atttgatcca
//
LOCUS       DATDUN010000447         1966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_311659, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000447 DATDUN010000000
VERSION     DATDUN010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311659"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VJC18_06260"
     CDS             complement(<1..504)
                     /locus_tag="VJC18_06260"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HLD45021.1"
                     /translation="MSGFFSITSGSTNETKARCGEIHTKHGRIKTPIFMPVGTAGTVK
                     AITPQMLKELSAQIILGNTYHLFLRPGHELIQKAGGLHKFMAWDGPILTDSGGFQVYS
                     LAKLRRVTDDGVTFQSHIDGTEYHLSPEKSIEVQEALGSDIMMCFDECPGLPATRKKI
                     EESLALTL"
     gene            1384..1932
                     /locus_tag="VJC18_06265"
     CDS             1384..1932
                     /locus_tag="VJC18_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HLD45022.1"
                     /translation="MAIARTTFSKASKVTKNFLASLPFKVGDVAVYPAHGVGEIKSIE
                     SREISGSTHQFYVLQILDNGMKIMVPTKNINAVGLREIISKDEVEAVYEILKERDITI
                     DNQTWNRRYREYMDKIKTGSIYEISEVYRDLMLLKHEKDLSFGERKLLDSARNLLVKE
                     LAFAEHRDEDEIEIDLQDIFDE"
BASE COUNT          517 a          495 c          418 g          536 t
ORIGIN      
        1 caacgtcagc gccaaggatt cttcgatttt ttttcgggtg gcgggaagcc ccggacattc
       61 atcgaaacac atcatgatgt cggatcccaa ggcctcctgc acctcaatcg atttttcggg
      121 tgagaggtga tattctgtcc cgtcgatgtg tgattgaaag gtcaccccat cgtctgtgac
      181 ccgcctgagc ttggccagac tgtagacctg aaaccctcct gagtccgtaa gaattggccc
      241 atcccaggcc ataaacttgt gcaggccgcc ggccttttgg atcagttcgt gcccgggtcg
      301 cagaaacaaa tgataggtat ttcccagaat aatttgggca gagagttcct tgagcatttg
      361 gggggtaatc gccttcacag tccccgccgt acccactggc atgaagatcg gtgtttttat
      421 acgaccatgt tttgtgtgga tttcaccaca cctggccttt gtctcattgg tggaaccgct
      481 tgtgatggaa aaaaagcctg acatgcggcg cttttaaata ataactcaga aaagagaaag
      541 aattatttct aaccatagac cgtttattgc cgtgaagcgt agatattaaa tgaaggcgct
      601 gtaaaagatc gatgccttca aagatttaaa gtttttttca tttagctctc cttaacaaaa
      661 gccccgaatt tggtgtggtt accaagcggg gctttttttt acccttttac ccttttgacc
      721 ttttgacctt ttgacccatt gaccctttga ccctttgacc ctttgaccca ttgacccatt
      781 gaccttttga ccctcctccc cacacacaat ccccaattgc taactgcggc aattgcccat
      841 gacctcccct tcgtccctcg tcccttcgtc cttcgtccct cgtccttccc ccttcccaca
      901 tccctttact tgtgttaaca tgagtataaa gaaaagagca ttttatgact tttttcacgc
      961 aacatccctg tgaagttgcc gataactatt atgtacgtca tggggcgaac acgggggcct
     1021 catgataaac aaagtttgtt gacagcgatt gggtcgattt tcataagtct ttttaagggg
     1081 ttactcatta tccccatccg ctttcaacgc aacctaatat caccgctttg cgtgtgatga
     1141 ctcacatgtc aaattggcgg aaacaaattt gtcatatgtt gttattgaac atttcatcta
     1201 tcaacaaaac atccattttt gttcaaaaaa caacattaat tacccctgag gctattgatt
     1261 aatacatttg gttgtgttat acgcttccct caaatttcga ggggcgagac ataaaccaat
     1321 aataacataa tgattacagc cggttagcag agccgaatag caactttttt tggagatcga
     1381 tccatggcaa tagcaagaac cacgttcagc aaggcatcga aggtaacaaa aaattttctg
     1441 gcttctttac cgttcaaggt aggcgatgtg gccgtttacc ccgcccatgg ggttggcgaa
     1501 atcaaaagca tcgaatcccg cgaaatttcc ggaagcacac atcagttcta cgttctgcaa
     1561 attctcgaca acggcatgaa gatcatggtg cccacaaaga acatcaacgc cgtgggtttg
     1621 agggagatca tctccaagga cgaagttgag gccgtgtacg aaatcctcaa ggaacgcgac
     1681 atcaccatcg acaaccagac ctggaaccgt cggtatcgcg aatacatgga caaaatcaaa
     1741 accggttcca tctacgaaat ctccgaggtc tatcgcgacc tcatgttgct caaacacgaa
     1801 aaagacctct ccttcggcga acgaaaattg ctggactccg cccgcaacct gctcgtcaaa
     1861 gaacttgcct tcgcggaaca ccgcgacgaa gacgaaatcg aaattgatct gcaggatatt
     1921 tttgatgagt gatgtgcttt gtggatcgtg gatcgtggat cgtgga
//
LOCUS       DATDUN010000448         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_228183, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000448 DATDUN010000000
VERSION     DATDUN010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228183"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VJC18_06270"
     CDS             <1..374
                     /locus_tag="VJC18_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HLD45023.1"
                     /translation="DSRVYLVRGDNIKQLTSDHSLVSEQMRAGLISENDAKKHKLKNI
                     ITRSVGYQEEVEIDVQKFELLPHDKIVLCSDGLTNMVDDENLRLIIHANPVEDACRKL
                     IGRANDNGGEDNITVVVCGIQ"
     gene            499..1449
                     /locus_tag="VJC18_06275"
     CDS             499..1449
                     /locus_tag="VJC18_06275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLD45024.1"
                     /translation="MLKDYHIIFVPKDKRKTRTFKVSGLTLKVLLVTFILSIPLFFIS
                     ILSMIHYQNNLIALKRNNYENKKLIENKQVLVGRLAHLEKKLSLIDDSIGHLGEIMDI
                     DPQSLRFGTGPIDDMDYSLPEDFNEEFAGVGEAEEMIDDWMSENGNLTISKVNRKMNT
                     YQDDVQVLSKKLEEIYAINRDKINYVNATPSTLPTEGWITSGFGMRKHPIGRSYRMHA
                     GVDVASPVGTAVKAPASGRVVFAGRSGGYGQTLVLQHGYGITTIFAHLNKMDVNKGTY
                     VKKGEQLGEVGNTGYSTGSHLHYEVHVDGIPTDPLAFVVQ"
     gene            complement(1481..2227)
                     /locus_tag="VJC18_06280"
     CDS             complement(1481..2227)
                     /locus_tag="VJC18_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45025.1"
                     /translation="MNETLKSLLKLSEVDKIIHHLSCQQEELPQINLELKTKIATLQN
                     ELNTKKIELSDLSALRKELLEAVQTKTEWMQSREERINELKTQKEYQAAQKEISLAKK
                     GIKEKDAQLGEVGPKIDRLTNELLTLTEKSEPLIAQHTSDIQSNNEKLGSLDKELEKN
                     TALRTELASNITDKTCLKHYEHIFRRAVPAISRLEGNICTECGTKVLPQSINMLKVGQ
                     QMQYCSRCKRILYIESLVFANDTDCPSCEG"
BASE COUNT          599 a          503 c          613 g          680 t
ORIGIN      
        1 gggattcgcg ggtgtatttg gtgcgaggag acaacatcaa acagctcacc agcgatcatt
       61 cactggtttc cgagcagatg cgagcgggtt tgatttcgga aaatgatgcc aaaaaacaca
      121 aactcaaaaa catcatcacg cgttcagtcg gttaccagga agaggtcgag attgatgttc
      181 aaaaattcga gcttttgcca cacgataaaa ttgtgctgtg ttctgatgga ctcacgaata
      241 tggtagacga cgagaactta agactgatca ttcatgcgaa cccagtcgaa gacgcgtgtc
      301 gcaaattgat cggtcgagcc aacgacaacg gtggggaaga caatatcaca gtggttgtgt
      361 gtggtattca gtgattttgg ccaaaccgtt gaaatcgctg agttcgtgag tattgcggac
      421 gaactgcgat tctcgaccta aattccgcaa gtcggaattc aggcaacttt tgccgaaaag
      481 agacgatcat aagttaggat gttaaaagac tatcacatca ttttcgtccc caaggataag
      541 cgcaagacga gaacattcaa ggtgtctggg ttgacactca aagttctgtt ggtgacgttt
      601 atcctctcca ttcccttgtt cttcatttcg atcctgtcga tgattcatta tcaaaataat
      661 ttaatcgcac tcaaacggaa caattacgaa aacaagaaac tcattgagaa caagcaggtg
      721 ctcgtgggca gactggctca cttggaaaag aaattgtcgt tgatcgatga cagcattggt
      781 catttgggcg agatcatgga cattgatccg cagtcgctca ggtttgggac aggtccgatc
      841 gacgatatgg attattcgtt acccgaagat tttaacgaag aatttgccgg tgtcggtgag
      901 gccgaagaaa tgatagatga ctggatgtca gaaaatggca acctcacgat cagcaaggtg
      961 aatcgcaaga tgaataccta ccaggatgat gtgcaggtgc tgagcaagaa gctggaggag
     1021 atttacgcca tcaatcgcga taaaatcaat tacgtcaacg cgactcccag taccttgccc
     1081 acggaggggt ggatcacttc cggatttggt atgcgcaaac accccattgg acgtagctac
     1141 cgcatgcacg cgggtgttga cgtggccagt cctgtgggaa ctgccgtcaa ggccccggcc
     1201 agcggcaggg tagtgtttgc gggtcgtagc ggtgggtatg gacagacgtt ggtgctccaa
     1261 cacggttatg gcatcacaac cattttcgcc catctcaaca aaatggacgt caataaaggc
     1321 acttatgtga agaagggtga acagcttggc gaggttggta acacaggcta ttccacaggg
     1381 tcacatctgc attacgaggt tcacgtcgat ggcatcccca cagatcctct ggcgttcgtt
     1441 gttcaataat gatcactgct atttgagttt atcatccccg ttatccttcg caggaaggac
     1501 aatcagtatc attcgcgaaa accagtgact caatgtagag gattctcttg caacggctac
     1561 agtactgcat ttgctgaccg actttgagca tgttgatgga ttgaggcaat accttggtcc
     1621 cgcattccgt gcagatgttg ccctctaagc gtgagatggc aggaacggcg cgtctgaaaa
     1681 tgtgttcgta gtgtttgagg caggttttgt ctgtgatgtt gctggccaat tcagtacgca
     1741 aggctgtgtt tttttcgagt tctttgtcca gggaaccaag tttctcgttg ttgctctgga
     1801 tgtcgctggt gtgttgggcg atcaagggtt cgcttttttc ggtgagggtc agtaattcgt
     1861 ttgtgagtct gtcgattttc gggcccactt cgccaagctg agcatctttt tctttaattc
     1921 cctttttggc cagagagatt tctttttgtg ccgcctgata ttctttttgt gttttaagct
     1981 cgttgatgcg ttcttcacgg gattgcatcc attctgtctt ggtctgaaca gcttcaagca
     2041 gttctttcct taaagcgctc aaatcgctca attcgatttt cttggtgttc aattcgtttt
     2101 gcagggtggc aatttttgtt tttaattcaa ggttgatttg tggcagttct tcttgctgac
     2161 aggaaagatg gtggatgatt ttgtcgactt ctgagagctt gagcaagctt ttcaatgttt
     2221 cattcacaat ggcatcctcg aaggttaagt tttcaggaaa acagccagaa acgctgtttt
     2281 cttagcaaga gatcagggcc gcatttctag tctagttgct gtcgaaatgc aaatggtttt
     2341 cattaaatgg ggggtgtacg acataaaagt aggtaatttt gattttgccc ccccc
//
LOCUS       DATDUN010000449         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_355552, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000449 DATDUN010000000
VERSION     DATDUN010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355552"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..342)
                     /locus_tag="VJC18_06285"
     CDS             complement(121..342)
                     /locus_tag="VJC18_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45026.1"
                     /translation="MKLRIFIPMVLVLMVTFGLSCDRSNEQQGSVADFSAVTNVQGTG
                     LSGWIATFIRKLLSEKPKPVDEDLVRDEV"
     gene            complement(354..806)
                     /locus_tag="VJC18_06290"
     CDS             complement(354..806)
                     /locus_tag="VJC18_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45027.1"
                     /translation="MMTVRKIIFCVTLIGAALVSLQVSAGNPAPLFYEPVHLGGESHY
                     PVKKKTPTPAPTQEQTQVQTETPTEVQTAEQAPVTIPATTTDTVTATPAETNTTTTTT
                     TTTTTAPSPADPVATTTTTTPSAESTTATTETGPAPTVPGEKKEEFDF"
     gene            complement(837..1166)
                     /locus_tag="VJC18_06295"
     CDS             complement(837..1166)
                     /locus_tag="VJC18_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45028.1"
                     /translation="MKIAIMSLFFVLAWSVPCVLVREAQAGNPLPLFDQPVKLDDSGR
                     PLYNSWEKQEYMRKLKARNKARHDRGQQDAGSVSTESQRTKENLAEPTPSSAVINRPM
                     TDEPRTD"
     gene            complement(1265..>2576)
                     /locus_tag="VJC18_06300"
     CDS             complement(1265..>2576)
                     /locus_tag="VJC18_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HLD45029.1"
                     /translation="IFFRKYKFELTYGGIDFFHSDPSDQAWASHMDYRKGRRAQQKKT
                     LFVKAVFDMAYVAHICLSGLKYLGPSYLRAFADQLIAVLGKRLLQLSESSLSVLGAEK
                     LQGLQGKFILIFNHKSAFDFALTPYVLSHITVNNRRVRPRFILAQDHFKDNWFLYHVI
                     GMGKAAEAIDMIFVSRKDQKKSFDNLKQAAQTMIEKDVDIAIYPQGTRAAGNYDRADK
                     RRDAGYYTTVRKKDLNSKWPHLKRGTAFLALDVLTQLRVSGDDQELHLVFIGISGTAT
                     VLPKGSLAVQTEAEVVFTIGDVLSLSPHMLDDLFVADQPREEAPLARKKFATELTGVI
                     NEKLLSVLNLKFQLLQRFLTELKGQFHYDNERLQVVQNNMVMIDKSSDVVFEILDKIY
                     SLPVSQWNGYLSQLSQMLLGKPDLTRFEILAHDVTEDLLSLKAK"
BASE COUNT          611 a          681 c          581 g          703 t
ORIGIN      
        1 tttgcgtaga ttgggacaag acgggtcaga atttttttgt taagggcatc tctcaacaca
       61 ataacagctc attgcttgat tgtttcattg cttgattgct tgattgtttg attgttctcc
      121 ctacacctca tccctcacca aatcttcatc gacgggtttg ggtttctcgc tcaaaagttt
      181 cctgatgaac gtcgcgatcc aaccgctcag accagtaccc tgcacatttg tcacagcgga
      241 gaaatctgct accgatcctt gctgttcatt cgatcggtcg caagacaaac caaacgtcac
      301 catcaaaaca agcaccatag gaatgaaaat tcgcaatttc atagcgatct caattaaaaa
      361 tcaaattcct cttttttttc acccggcacg gtcggtgctg gccctgtttc tgttgtggcc
      421 gttgtcgact cagcacttgg tgttgtcgtg gtggtggtgg ccactggatc cgcaggtgat
      481 ggcgctgttg ttgttgtggt agttgtagtc gtcgtggtgt tcgtttccgc aggggttgcc
      541 gtcactgtat ccgtagtggt cgccggtatt gtcactggcg cctgttccgc agtctgtacc
      601 tctgttggcg tttctgtctg aacttgggtt tgttcctgcg taggggctgg cgttggtgtc
      661 ttcttcttta cgggataatg gctctcgccc cccaaatgca ccggctcgta aaacaggggg
      721 gcaggattgc ccgcgctcac ctgcaaactc acgagagcgg ctccgatgag tgtgacacaa
      781 aaaataattt ttcgtactgt catcatgttt ttctccttat agaaaatcag cacgcctcaa
      841 tctgttcttg gctcgtccgt catgggccga ttgataactg ctgatgatgg tgttggttct
      901 gccagatttt cctttgttct ctgtgactcc gtcgacaccg atccggcatc ttgttgccct
      961 cgatcatgcc gggccttatt cctggccttg agcttcctca tgtactcctg tttctcccag
     1021 gaattgtaca acggtctgcc ggaatcgtct aatttcaccg gttggtcaaa aagaggcagg
     1081 gggttaccgg cttgggcctc tcgcaccaag acacagggaa cactccaagc aagaacaaag
     1141 aaaagtgaca tgatcgctat tttcaaaact tgcccctgca acacaacctc ccccattcat
     1201 ttttgccctc aatttgtatt atcatcagta gcaaatcgtc aacgaaacaa tcacggcaat
     1261 tggtctattt cgccttcagg gacaacaggt cttcggtcac atcatgcgcc aggatttcga
     1321 accgcgtcaa atcaggtttg ccaagcagca tctggctcaa ctgcgacaaa tacccattcc
     1381 actgtgatac gggcaacgaa tagatcttgt cgagtatctc aaaaacaaca tcactgcttt
     1441 tgtcgatcat caccatgttg ttctgcacca cctgtagcct ctcgttatca taatggaact
     1501 gtcccttcaa ctctgttaaa aaacgctgga gcaattgaaa ttttagattg agcacgctta
     1561 acagtttttc gttgatcaca cctgtgagtt cagtggcaaa ttttttgcgg gccaaagggg
     1621 cctcttctcg cggttgatcg gccacaaaca aatcatcaag catgtgcggt gacaaactca
     1681 atacatcacc aatcgtaaac acgacctcgg cctcggtctg gacggccagg ctccccttgg
     1741 gtaaaacagt ggctgttccc gagataccga taaaaaccag gtgcagttcc tgatcatcac
     1801 cgctgaccct gagttgcgtc aaaacatcaa gcgccaaaaa agctgtcccg cgcttgaggt
     1861 gaggccattt cgagttgagg tcttttttac gcaccgtggt gtaataaccg gcatctcgtc
     1921 tcttatcggc ccggtcataa ttccccgccg cccgcgtgcc ttggggataa atggcaatgt
     1981 cgacgtcctt ctcgatcatg gtttgtgccg cctgtttgag gttgtcaaaa ctcttttttt
     2041 gatccttgcg cgacacaaaa atcatgtcaa tcgcctccgc ggccttgccc atcccgatca
     2101 catgatacaa aaaccaatta tccttaaaat gatcctgtgc cagaatgaac cgtggccgaa
     2161 cccgccggtt gttcactgtg atgtgagaca acacataggg ggtcagggcg aaatcaaagg
     2221 cactcttgtg attgaaaatc aaaataaact taccctgcaa accctgtaat ttctcagccc
     2281 ccagaacact gagcgaggac tcactcaact gcagcagtcg tttgcccaac acggcaatga
     2341 gttggtcagc gaaggccctc aaataggatg gtcccaggta tttgagtccc gacaggcaaa
     2401 tatgagccac ataggccata tcgaacacgg ccttcacaaa aagtgtcttt ttctgctggg
     2461 cacgcctccc ctttcgataa tccatgtgtg aggcccaagc ctgatcactg ggatcgctgt
     2521 gaaaaaaatc aatcccccca tacgtgagtt cgaacttgta ttttctaaaa aagatg
//
LOCUS       DATDUN010000450         1196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_560479, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000450 DATDUN010000000
VERSION     DATDUN010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560479"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /gene="hemH"
                     /locus_tag="VJC18_06305"
     CDS             <1..936
                     /gene="hemH"
                     /locus_tag="VJC18_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HLD45030.1"
                     /translation="PTLPAIRAFLEEFLTDPFVIDKQNGWWRFLLKTTILPKRSKALL
                     PKYQSLFQKPNQPLTKATQSICQKIYEQLSGSNPFLVVRWAARYGEPSIQNILTTMVN
                     DEQCDQIIVLPLFPQYSHTTTGSIQNQVKLALHSLRQKPKVKFISSFCQHSSYIHSLC
                     ELIQPCLALTRPERLILSYHGLPKRYTALGDPYIGMCESTSAHIKETLNYPAKHILTT
                     YQSRFGKEEWSGPDTLTTLSQLAKQGIKNVAIACPSFTIDCLETLDEIDVAGREIFLD
                     HGGKTFTYIPCLNDDPQWMFALSDFLKQELLSFKT"
BASE COUNT          321 a          350 c          221 g          304 t
ORIGIN      
        1 cccacccttc ccgctatccg cgcctttctg gaagaatttt tgacggaccc gtttgtgatc
       61 gacaaacaaa atgggtggtg gcgctttttg ctcaaaacca cgattttgcc aaaacgatca
      121 aaggcattgc tccccaagta ccaatccctt tttcaaaaac ccaatcaacc cctgaccaag
      181 gccacacaaa gcatctgcca gaaaatttat gaacaactca gcggctcaaa cccttttctc
      241 gtcgttcgtt gggccgcgcg ctatggagaa ccaagcatcc agaacatcct caccaccatg
      301 gtcaacgatg agcaatgcga tcaaatcatc gtcctgccct tgtttcccca gtacagccac
      361 acgacgaccg gctccataca aaaccaggtc aagctcgcat tacactccct gcgccaaaaa
      421 ccaaaagtca aattcatcag ttcgttttgc cagcacagca gttacattca cagcttgtgc
      481 gaacttatac aaccgtgtct tgccttaacc agaccagagc ggcttatcct ctcgtatcac
      541 ggtctgccca agcgctacac agcactgggg gacccctaca ttggcatgtg tgagtccaca
      601 tcggcgcaca tcaaggaaac actgaactat cccgccaaac acattctcac cacctaccaa
      661 tcccgttttg gcaaagaaga atggtccggt cccgatacct tgaccacgct gagtcaactg
      721 gccaaacaag gcatcaagaa tgtggccatt gcctgcccca gctttaccat tgattgtctg
      781 gaaacgcttg atgagattga tgtggcgggt cgtgagattt ttttggatca cggcggcaag
      841 accttcacct acatcccctg cctcaatgat gatcctcaat ggatgttcgc gttatccgat
      901 tttctcaaac aggaattact cagttttaag acttagaatg cctttatggc acctgaatcc
      961 tcatcatttc ttcattttca gacaccgtga attccatctg aatttcatct caattcacgt
     1021 tattttgtca agataccgtg aattgagatg aaattcagat caaattcacg ctgtttttcc
     1081 aacacaaaaa aaaccgcccc tgctgttctc aacaagggcg gttgctattc tcatatttgg
     1141 caccaaagtg ccctattatt gctttaggct caaccatcat tcccgatcgc ttcgac
//
LOCUS       DATDUN010000451         3763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_271330, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000451 DATDUN010000000
VERSION     DATDUN010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271330"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..501)
                     /locus_tag="VJC18_06310"
     CDS             complement(73..501)
                     /locus_tag="VJC18_06310"
                     /inference="COORDINATES: protein motif:HMM:NF040319.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HLD45031.1"
                     /translation="MTKKRYLKDKIYLRITLKSNCVFLRSDFEDLGDYDQVGRVLKNL
                     VQESKIIKLGYGLYARAEISSLNGQVIPEKNLPELAAEALKRLGVTVTTSSSEKEYNA
                     GLSTQVPTGRLIAVKGRVVRKIGFGDVYVDYERISEQTIV"
     gene            complement(637..1317)
                     /locus_tag="VJC18_06315"
     CDS             complement(637..1317)
                     /locus_tag="VJC18_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HLD45032.1"
                     /translation="MTSLLINIGAAFWLGILTSISPCPLATNIAAVSFLGRRVQTPVK
                     VFWAGLLYTAGRTMTYVVLGMLLVSSLLSAPTLSHVLQKYMHTVLGPVLIVVGLILVG
                     IIRINISGLGLGNKMQKRIEALGLWGALVFGVLFALSFCPVSAALFFGSLLPLAVQQH
                     SFFILPLVYGIATGLPVLVFAVLIASGAKRVALMLNKLTAFEKWARIVTGVILIVIGI
                     YETALIAL"
     gene            complement(1314..1796)
                     /locus_tag="VJC18_06320"
     CDS             complement(1314..1796)
                     /locus_tag="VJC18_06320"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HLD45033.1"
                     /translation="MKTRNIVLVVLLLLVGLSFAGTYFKQDNSIASSVAAPVSVSEVK
                     MEKGVAAYYFHGTRRCPTCRKIEALTNEAIQTGLATDIKDGRLVWNVLNIDEDANNHF
                     IQDFQLTSSSVILVRYDGGESKKWKNLDKVWRLVRSDKNQFVSYVQSEIKSYLESPAQ
                     "
     gene            complement(1811..2833)
                     /locus_tag="VJC18_06325"
     CDS             complement(1811..2833)
                     /locus_tag="VJC18_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HLD45034.1"
                     /translation="MKKTLFAFVFPLSAFLWWLIYSHLAAVSKFLTYTVIGLSPATHL
                     GLAVEFFVFEVPKVLMLLVVVVFGVGIIRSFITPERTRQILAGKKEFVGNVLAALLGI
                     ITPFCTCSAIPLFIGFLTAGVPLGATLSFLISAPMVNEVALVLLFGLFGWKVALLYMS
                     TGLTIAIVAGWIMGRCYLEKWVEPWVLQTQTGAVQNISSAHTTWNLRFQYAVTAVRDI
                     VGKVWVYIIIGIAVGAGIHGFVPQDFMASIMGKSAWWAVPIAVLIGVPMYSNAAGIIP
                     VVQALLGKGAALGTVLAFMMAVIGLSLPETLILRKVLKLPLILTFVGIVALGILLTGY
                     LFNWIL"
     gene            complement(2839..3135)
                     /locus_tag="VJC18_06330"
     CDS             complement(2839..3135)
                     /locus_tag="VJC18_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLD45035.1"
                     /translation="MEIKVLGPGCPNCKTLAKRTEEAVQNMGIDAQIIKVTDYPEMMK
                     YDMLQSPGLVINEKLVMSGRVPTVAEITTLLANAELGASAELSASACGCKKGCC"
     gene            complement(3195..>3763)
                     /locus_tag="VJC18_06335"
     CDS             complement(3195..>3763)
                     /locus_tag="VJC18_06335"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLD45036.1"
                     /translation="AKLTGPKGRVIGVDMTPAMIERARANAIAAGLSNVEIREGIIEK
                     LPVDDDSVDLVISNCVINLSPEKQKVFSEIHRVLKDGGRFSISDIVAEPMPEWIIKKG
                     TLHSACISGAIPETDYLEGLAKAGLVDVAVRDRLVYGESELLGLLDSELTGSCCCGTP
                     SIPKNELTRIAKELAGQVASIRVVGRKI"
BASE COUNT         1103 a         1001 c          780 g          879 t
ORIGIN      
        1 ttgagggctt gtgtgacatg ccagtctttt tcaacaaaag actcgctaat gcccatctgt
       61 ctggccgcca gttcaaacaa ttgtttgttc gctgattctt tcatagtcga cataaacatc
      121 tccaaaacca atttttctca ccacacgtcc cttgactgca atgagcctac cagtaggcac
      181 ctgtgtagac aggcccgcgt tgtattcttt ttcggacgat gatgttgtca cggtcacgcc
      241 cagtcgtttc agcgcttctg ctgccagttc tggtagattt ttttctggaa tcacctgacc
      301 gttcaaagaa gatatttcgg cacgggcata taacccataa ccaagcttga taattttact
      361 ctcttgcaca agatttttta acacccgccc cacctgatca tagtctccca aatcttcaaa
      421 atctgaacgc aaaaacacgc agttactttt aagagtaatc ctgagatata tcttatcttt
      481 taaatatctc ttttttgtca tatattacct ataactggga atacaaaaac acgacattat
      541 cacaaaacaa atatacgaca ttttttcgtt tttagcaatt caatttatca acccgcacct
      601 gacgcggggc aaccctttga atttctttgt ctcgactcac aacgcaatca acgctgtttc
      661 ataaattcca atgacaatca gaataacccc cgtcacaatg cgtgcccact tttcaaatgc
      721 ggtcagtttg ttgagcatca gggccacccg tttggcacca gaagcaatga gcactgcaaa
      781 cacgagcacg ggtaatcccg tggcaattcc gtaaaccaac gggagaatga aaaaagaatg
      841 ctgttgaaca gcgaggggca acaggctccc aaaaaacaag gcggccgaca cgggacaaaa
      901 ggagagagca aaaagaacac caaaaaccaa ggctccccac aaacccaatg cttcaatgcg
      961 tttctgcatc ttgttgccaa ggcccaaacc agagatatta atgcgaatga ttcccaccaa
     1021 aattagacca accacaatca gcacaggtcc cagcacagtg tgcatgtatt tttgcaacac
     1081 gtgcgaaagg gtgggggcac tcaagaggct tgagaccagc aacatgccca acacaacgta
     1141 ggtcatggtt ctgcctgctg tgtaaagaag gcctgcccaa aacaccttta ccggtgtttg
     1201 cactcgccgc cccaaaaacg acacggcagc aatgttggtg gccaacggac aggggctgat
     1261 tgacgtgaga atacccagcc aaaaggccgc gccaatgttg atgagcaagc tggtcattgt
     1321 gcgggactct ccaaataaga tttgatctcg ctttgaacgt agctgacaaa ttggttctta
     1381 tcactcctga ccaacctcca aaccttgtcc agattcttcc attttttgga ttcgccgcca
     1441 tcgtatcgaa ccagaatgac agaacttgag gtcagctgaa agtcctgaat aaagtggttg
     1501 ttggcatctt catctatatt caacacattc cataccaagc gcccatcctt gatgtctgtg
     1561 gcaaggcctg tctggatggc ctcgtttgtg agagcctcaa tcttgcgaca ggttgggcaa
     1621 cgccgtgttc catgaaaata ataggccgca acaccctttt ccatttttac ttcggataca
     1681 gaaactggag cggcaacact gctcgcgatt gaattgtctt gcttgaaata cgtgcccgca
     1741 aaactcaacc caaccaacaa aagtaaaacg actaggacaa tgtttcgtgt tttcatgagc
     1801 aacagcctcc tcagagaatc cagttaaaaa gatagcctgt cagcaaaatc cccaaggcca
     1861 caataccaac aaaggtaaga atgagcggga gcttgagcac ttttcttaaa atcaaggttt
     1921 cgggtaacga caacccaatc acagccatca taaaggccaa cacagtgcct aaggcggcac
     1981 ccttgccaag aagcgcctgc accacaggaa tgatacctgc cgcgttggag tacatgggga
     2041 caccaatcag caccgcaata ggcacggccc accaagcgct tttgcccatg atcgatgcca
     2101 taaaatcttg cggcacgaac ccatgaatcc ctgcccccac ggcaatacca atgatgatgt
     2161 acacccacac cttgccaaca atatctctaa cggccgtgac agcgtattga aaacgcagat
     2221 tccaggtggt gtgcgcgctt gaaatgtttt ggactgctcc agtttgcgtt tgcaaaaccc
     2281 aaggttcaac ccacttttca agataacacc tgcccataat ccaaccagca acaatggcaa
     2341 tggtcaggcc cgtgctcatg tagaggagag ccaccttcca cccgaatagc ccaaacaaca
     2401 agacaagtgc tacctcattc accatggggg cagaaatgag aaacgaaagc gtggcgccta
     2461 aaggaacccc cgctgtgaga aaaccaataa acaagggaat ggcagaacag gtacaaaaag
     2521 gcgtgatgat gcccaacaaa gcggccagca cgttacccac aaactctttt ttgcccgcca
     2581 gaatttgacg tgtccgttca ggggtaatga aagacctgat gataccaaca ccaaaaacaa
     2641 ccaccaccaa aagcatgagg accttaggca cttcaaaaac aaaaaactca acagccaacc
     2701 ccaaatgcgt tgctggcgaa agaccgatga ccgtataggt caaaaatttt gaaactgcgg
     2761 ctaggtggct ataaataagc caccacaaaa aagcggacaa aggaaagaca aaagcaaaaa
     2821 gagttttttt cataaatgtt agcaacaccc ctttttacaa ccacacgcac tggccgacag
     2881 ttcggcggat gcacccaact cggcattggc gagtaacgtc gtgatctcgg ccaccgtagg
     2941 tacgcgacca ctcatgacga gtttttcgtt gatgaccaga cctggcgatt gaagcatgtc
     3001 gtatttcatc atctcgggat aatcagtcac cttgatgatt tgagcgtcaa tgcccatgtt
     3061 ttgcacggct tcttcggtgc gtttggcaag tgttttacaa ttagggcaac cggggcccaa
     3121 tactttgatt tccataacaa gctccttatt cattatttat tgttttcaaa aaagaatgcc
     3181 tttcatactg tctcctatat cttgcgcccc acaacccgaa tgctggcaac ctgaccagca
     3241 agttcctttg caatacgagt cagctcattt tttggaattg agggtgttcc gcaacaacag
     3301 gaacctgtca gctcggaatc caacagaccc aagagttccg attcgccata aaccagacga
     3361 tctcgcacag ccacatcaac caaacctgct tttgcaaggc cttccagata gtctgtctca
     3421 ggaatggccc ccgatatgca ggccgaatgg agagttcctt ttttgatgat ccactcgggc
     3481 atgggttctg ccacaatatc tgaaatggaa aatcgaccgc cgtctttaag cacacggtga
     3541 atctccgaaa acaccttttg tttttctggc gaaagattga tcacacagtt agaaataacc
     3601 aaatcaaccg aatcatcgtc cacaggcagt ttttcgataa tcccctcgcg tatttcaaca
     3661 ttggaaagcc ctgccgcgat agcattggcc cgtgcgcgct caatcatggc tggcgtcata
     3721 tccacaccaa tgacccgtcc ttttgggcca gtgagcttgg cgg
//
LOCUS       DATDUN010000452         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_437988, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000452 DATDUN010000000
VERSION     DATDUN010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437988"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VJC18_06340"
     CDS             <1..379
                     /locus_tag="VJC18_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45037.1"
                     /translation="RAKAESCKDVASQSTTGRADDMPVTQETSKVAWASLIARIFGDK
                     PTQCPQCGDEMRLMGFEFEARFLHDISQMQRAPPKFKVVRYAELPTQPVVERAQNGAD
                     EQTAVVSGEIMPNYEIVDQSISW"
     gene            complement(636..2033)
                     /locus_tag="VJC18_06345"
     CDS             complement(636..2033)
                     /locus_tag="VJC18_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45038.1"
                     /translation="MFFNRTKELRDLDTLWRSGEAQLIVLYGRRRVGKSSLLKAWIKN
                     KRGLYWTATATTNERLLQSFSATLYLFKNPQAEIPPDFSYPNWESALVETATLANKQK
                     IAIVIDEFPYLIEANAEIPSLLQKIWDAHFEKSKALLTLTGSRVGMMEKHVLSSRGPL
                     YGRASSVLWLNPLPFNELHHFLPRYSKTQLVEIYAITGGVPLYIKMFDDSVPVLANIQ
                     RELSSTTSLLKGEPYFLIHEELKEPLRYVAILEALGLGKKHISQMSATTGIEKTHLIP
                     YLKTLETLRYIKRNIPITENKNVSRKGLYDFSDLFLKFYFRFIAPHQTLLEDGLEKAV
                     FKTITDQFDSFVGKEGFEALCRQWLVSQADQNKLPFNPDVIGKYWDSNIEIDLAALNK
                     KQQALIVGEAKWTNEKCGLGVLQELNRKTEYINKKFGFHINKFIFSKAGFAAPLIERA
                     RIENVRLIGLDEIFK"
BASE COUNT          526 a          443 c          540 g          629 t
ORIGIN      
        1 acgggccaag gccgagtcgt gcaaagatgt tgcgtcacaa agcacgaccg gcagggctga
       61 tgatatgccg gtgacacagg agacatccaa agtggcttgg gccagtctga ttgcccggat
      121 ttttggcgat aagccaaccc agtgccccca gtgtggtgat gagatgaggc tcatgggttt
      181 tgagtttgaa gcaaggttct tgcacgatat ttctcagatg cagagggcgc cgccaaagtt
      241 caaggttgtc agatacgctg agttgccgac acagcccgtt gtggaacgag cacagaacgg
      301 ggcggacgag caaacagcgg tggtttctgg tgagatcatg cccaattacg agattgtgga
      361 ccagtcgatt tcatggtagt ggcagaggtt tgctctgagc acagggcaaa aactgttttg
      421 tgatgcccat cgacaagttt ttatcgtgag ggttggttct ctatccaatt ttttgtcatt
      481 tcaacggtgc cactcgtgtt ttttttgact tgcgttggcc aatttttaaa tatatccgac
      541 tctcagtttg ataaagttcc taatgtacct tgatcgtgtg catcagttgg aaaattcgtt
      601 ttttcatccg aaaagcggtg atggccttat cgccgtcatt tgaagatttc gtccagtccg
      661 atgagacgta cgttttcaat gcgggctctt tcaattaaag gggctgcaaa accagccttg
      721 ctgaagatga atttgttgat gtgaaatcca aatttcttat tgatgtattc cgtttttctg
      781 ttgagttcct gcaagactcc cagcccgcat ttttcatttg tccattttgc ttcacccaca
      841 atcaaagcct gttgtttttt gttcagggct gccaagtcta tttcgatgtt gctatcccaa
      901 tacttgccaa tcacgtcagg attgaagggc agtttgtttt gatcggcttg tgacacaagc
      961 cattggcggc acagggcttc aaagccttct ttgcctacaa aggaatcaaa ttgatctgtg
     1021 attgttttga aaacggcttt ttccagaccg tcctccaaca gtgtctggtg gggggctatg
     1081 aaacgaaaat aaaacttcaa aaataaatcc gaaaaatcat acagcccttt tcggctcaca
     1141 tttttgtttt ctgtaatagg gatatttctt ttaatgtaac gcaaggtttc taatgttttt
     1201 aaatagggga tgaggtgtgt cttttctatg cccgtggttg ccgacatttg cgatatatgt
     1261 tttttcccca agcccaacgc ttccaggata gcaacgtaac gcaggggttc tttcagctct
     1321 tcatggatca aaaagtaggg ttcccccttg agaagtgacg tggtggagga aagttccctc
     1381 tgaatgttgg caagtacggg aacagagtca tcgaacattt tgatgtacag agggacccct
     1441 ccggtgatgg cgtagatctc tacaagctgg gttttgctgt aacgaggcaa aaaatgatgg
     1501 agttcgttaa agggtaaagg gtttaaccat agcactgaag acgctctgcc ataaagcggc
     1561 ccgcgtgatg acaacacatg tttttccatc atgcccacac gactgcccgt gagcgtgagc
     1621 agggcttttg atttttcaaa atgcgcgtcc cagatttttt gcaggagcga agggatttcg
     1681 gcgttggctt caatgaggta gggaaattca tcgatgacga tggcgatttt ttgtttattg
     1741 gccagcgtgg ctgtttctac caaggcagat tcccagtttg gataactgaa atccggggga
     1801 atttctgcct ggggattttt gaagagatag agggttgccg aaaaggattg aagtaacctt
     1861 tcgttggtgg tcgctgtggc tgtccagtag aggccccgtt tgtttttgat ccaggctttg
     1921 aggagtgaac ttttgccgac gcggcggcgg ccgtatagaa cgatcagttg agcctcgcca
     1981 gaccgccaca gggtgtcaag gtcacgcaat tcctttgttc gattaaaaaa caccagactc
     2041 cttttaataa ccgttaaatt atactaaaag aagtgtaact gtcaacacat taaacagatt
     2101 agttactata taattatgct aattgacgcg tcattgtt
//
LOCUS       DATDUN010000453         1400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_228364, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000453 DATDUN010000000
VERSION     DATDUN010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228364"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VJC18_06350"
     CDS             complement(<1..189)
                     /locus_tag="VJC18_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLD45039.1"
                     /translation="MPFAEKEKRIVALSSVLAAILLVGTKLVVGVTTNSLGILAEAAH
                     SGLDLVAALMTLWAVRVSD"
     gene            complement(192..>1400)
                     /locus_tag="VJC18_06355"
     CDS             complement(192..>1400)
                     /locus_tag="VJC18_06355"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HLD45040.1"
                     /translation="EGMEVKTDTDLIKKARASIMEFLLINHPLDCPTCDQAGECKLQD
                     YYMIHDAIPSRFAEKKVTKRKRIDLGAGVVLDEERCVLCRRCVRFCQQVAKRDELSVQ
                     GRGDQSFVSTFPDKPLTSPYAGCVVDICPVGALTSKDFRFKKRVWYLSQTDSICPGCS
                     RGCHITLWHEKGVVYRITPRFCAAVNQYWMCDVGRHDYRWLNEQRRFKAMTRVSGRLI
                     EASVNETLLRLKQLFESHDPFEVAFVASAKETSESIDAFVGFAKKRFGADFVYYSKKD
                     PPDPVADDFLVTRDKNPNLAHIYTLGLKTVDQMPQDIKAVVVQRDLSERDESVVRRRG
                     IEVLALLATNHTMLDDVAQVVLPVPTYAEQAGSFTNNNGCRQSFVAAFVPREGNGEAV
                     RLVADWMNVL"
BASE COUNT          340 a          416 c          321 g          323 t
ORIGIN      
        1 gtccgaaacc ctcacagccc aaagtgtcat gagcgccgcc accagatcga ggcctgaatg
       61 cgcggcctcg gccagaattc ccaggctgtt ggtcgtcaca cccacaacca acttcgtgcc
      121 caccaacagg atggcggcca aaacagaact taaggcgacg attctttttt ctttttcggc
      181 aaatggcata atcacaacac attcatccaa tcagcaacaa gcctgacagc ttctccgttt
      241 ccctctctgg gcacaaacgc ggccacgaaa gattgcctgc acccgttgtt attcgtaaag
      301 ctccccgcct gttctgcata ggtcggtacg ggcaaaacaa cctgcgccac atcatcgagc
      361 atggtgtgat tggtggcgag cagtgccagg acttcgatcc ctctccgtct gacgaccgac
      421 tcatccctct cgctcaaatc tctttgcacc acaacggcct tgatgtcttg aggcatttga
      481 tcgactgtct tcagtcctaa tgtgtaaata tgggccaaat tcgggttttt gtcacgtgtc
      541 acgaggaaat catctgccac gggatcgggg gggtcctttt tcgaataata aacaaaatca
      601 gccccaaatc tttttttggc aaaaccaaca aacgcatcaa tcgactcgct ggtctccttg
      661 gcactagcca caaaggccac ctcaaagggg tcatgagatt caaacaattg ttttaatcgc
      721 aacaacgtct cgttcacaga agcttcaatc aaccgtcctg acacacgcgt catggccttg
      781 aagcgtcgtt gctcgttcaa ccaccggtaa tcatgcctcc ccacatcaca catccaatac
      841 tggttcacag cggcgcaaaa gcggggcgtg attcggtaca ccacaccctt ttcgtgccac
      901 aaagtgatgt gacaaccccg tgaacaaccg gggcagatgg aatctgtttg cgatagatac
      961 cagacccttt tcttgaagcg aaaatccttt gaggtcagtg cccccaccgg gcagatgtcc
     1021 acaacacacc ccgcgtaggg gctggtgagt ggtttgtcgg gaaaagtgga cacaaaagac
     1081 tgatctccgc gcccctgcac cgacagctcg tcccttttgg cgacctgctg gcaaaaacgc
     1141 acacaacgac gacacaacac acagcgctct tcatccagca ccacgccggc acccagatcg
     1201 atcctttttc ttttggtcac ttttttctct gcgaaccgtg agggaatggc atcgtgtatc
     1261 atgtaatagt cttgcaactt gcactctccc gcctgatcgc acgtggggca atcaaggggg
     1321 tggttgatca gcaaaaattc catgatggag gcgcgggcct ttttgatgag gtcggtgtcg
     1381 gttttgacct ccatgccctc
//
LOCUS       DATDUN010000454         1188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_25184, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000454 DATDUN010000000
VERSION     DATDUN010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25184"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VJC18_06360"
     CDS             complement(<1..540)
                     /locus_tag="VJC18_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum hydroxylase"
                     /protein_id="HLD45041.1"
                     /translation="MQSKQILIKGAGEMASAVAHRLFRCGYSVVMADIAQPTAVRRRV
                     SFCSAIYEGEIEIEGVKGIFNQAIGNEQWATRNGKRETGDNHISILIDPQCHIKNILK
                     PDVIIDARLLKHNLDNHLTDAPLTIALGPGIEAGKDVHVVIETNRGHALGRIITKGFA
                     EADTGNPGVIGGYSLERVFR"
     gene            complement(544..765)
                     /locus_tag="VJC18_06365"
     CDS             complement(544..765)
                     /locus_tag="VJC18_06365"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45042.1"
                     /translation="MDDYLEQADLSAVFAKEGVAVQSKTRKINLDLPDSVVQKIDMIA
                     DKIGISRQPLIKLWIHEKIREEFAAEKSV"
     gene            complement(758..1051)
                     /locus_tag="VJC18_06370"
     CDS             complement(758..1051)
                     /locus_tag="VJC18_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HLD45043.1"
                     /translation="MYIFEWDSDKSQRNVVKHGVSFDEARQLWDSFHIEVKEVARAGE
                     EKRSATLGLIHGKVYVAIWTLRKNSIRIISVRRARTNEEKIYKKILQDNQRNG"
BASE COUNT          282 a          320 c          249 g          337 t
ORIGIN      
        1 acgaaaaacg cgttccaggg aataacctcc aatcacaccg ggattgcccg tatcagcctc
       61 cgcaaaaccc tttgtgatga tacgaccaag agcatgacca cgattggttt cgatcaccac
      121 gtgaacatcc ttgcccgcct caatgcccgg acccaaggca atggtcagcg gtgcgtcggt
      181 aaggtgattg tccagattgt gtttgagcag gcgtgcgtcg ataatgacat cgggtttcaa
      241 tatatttttg atatggcact gaggatcgat gaggatagaa atgtggttat cacccgtttc
      301 ccgtttcccg ttgcgcgttg cccattgttc attgcctatt gcttgattga aaatcccctt
      361 caccccctcg atctcaatct ccccttcata tatcgccgaa caaaacgaca cccgtctgcg
      421 cacagccgtg ggctgggcga tgtcggccat cacaacagaa taaccacacc gaaacaaccg
      481 atgagccaca gccgacgcca tttccccggc acctttgatg agaatttgtt ttgattgcat
      541 accttaaact gatttttcgg cggcaaattc ctcacggatc ttctcgtgaa tccagagttt
      601 gatcagtggc tgacgtgata tgccaatttt gtcggcgatc atgtcgatct tttggacaac
      661 cgagtcaggt agatcaagat tgatcttcct ggttttggat tgaaccgcca caccctcctt
      721 ggcaaacacg gcagacaaat ccgcttgttc aagataatca tccatttcgt tgattgtctt
      781 gaaggatttt tttgtagatt ttttcttcat ttgttctagc cctcctcaca ctaatgattc
      841 tgatactgtt ctttctgagc gtccaaatgg ccacatagac cttcccatga atcaacccca
      901 gagtcgccga gcgcttttcc tcacccgcgc gagccacttc cttcacttcg atatgaaagc
      961 tgtcccacaa ctgccgtgct tcgtcaaaac tcaccccgtg tttgacgaca ttcctctggc
     1021 ttttgtctga atcccattcg aatatataca taatcagtat attaattata ctttatttat
     1081 atcttatgat caatcacttt tagagcctat tcgaaaacct gccgcaaggc ggcctttttg
     1141 gggtgacagg gtgagctgtt tttgcgcgaa caggcgagca cgccgctg
//
LOCUS       DATDUN010000455         1385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_312100, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000455 DATDUN010000000
VERSION     DATDUN010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312100"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VJC18_06375"
     CDS             complement(<1..517)
                     /locus_tag="VJC18_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HLD45044.1"
                     /translation="MPTCTSKPIERVYVVYKRSVYQKYVLDLGNSTLSKLIKQKHPST
                     KTLFYTYDQHRKSLQLIRDVLNHYKIKYNISSRSISKNFSGYDLVITVGGDGTFLRTS
                     HYIDDQLMLGINSVPKFSVGAWCSITYKDFAKKFRDILAGKLRVRSYHRMTLTLNGHT
                     LPIEPVNDVLFC"
     gene            complement(966..>1385)
                     /locus_tag="VJC18_06380"
     CDS             complement(966..>1385)
                     /locus_tag="VJC18_06380"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45045.1"
                     /translation="TVCGFAGYRNIPATIHLSGPGLPNTQISGVNLTNLIVANGRYFG
                     RGMCPAPEASLNDGLFDVIIIKDVGWLGFLLNLRKMYARNKKVSSRIMETFRAQKITV
                     TSQDPKLLLPCEVDGETCGQGATVTFELLPKVISLLV"
BASE COUNT          332 a          290 c          355 g          408 t
ORIGIN      
        1 tacaaaataa aacatcgttg acgggttcga tgggcagcgt gtggccgttt aacgtgagcg
       61 tcatgcggtg atacgacctg accctgagct tgccagccag aatatccctg aatttttttg
      121 caaaatcctt gtaggtgatg gagcaccagg cccccacact gaacttcggc acggaattga
      181 ttcccaacat gagttggtca tcaatgtagt gtgaggtgcg caaaaaggtg ccgtcacctc
      241 ccacagtgat gaccagatcg tacccgctga agttctttga aatgcttctg gaggaaatgt
      301 tgtatttgat tttgtagtga ttcagcacat cgcggattag ctggagtgat ttgcggtgct
      361 ggtcgtacgt gtaaaacagg gttttggtgg aggggtgttt ttgtttgatg agtttggaca
      421 gcgtgctgtt gcccagatcg agaacgtatt tttgatacac gctacgtttg tacaccacat
      481 agacgcgttc gatgggtttt gacgtgcaag ttggcataga ggatttgttg tcagggaatc
      541 agtttgtttt tttgattctg gctcttcaat ttttgtctct gaattttttt aatattatca
      601 tgcggcggca aattcttgac catcaaatca gatttattgc ttttcatgag gtaacccttt
      661 gtaattcaaa aagaaccatc tcgaggatca cctcgagatg gttcttgaat aagtgaatat
      721 tgcaaaacgt tcatcggttt ggctgccaaa aacattatcc tttttttttc caataacgct
      781 catttttgaa aaaagaacat tgattgtata cggcagagta taactattgc gttacgatat
      841 ttatttcaca caactcgaat tgctccggga atgcgtatgt gttgttttgc tttcatgtgt
      901 gtttggcagt atagacgtgg cacctaggta gtcttaggaa atgcgattag tcaacatcat
      961 tattcttaaa ccagcagaga aatcaccttt ggcaacagct caaaggtgac ggtggctccc
     1021 tgcccgcagg tttcgccatc gacctcgcag ggtaatagaa gcttcgggtc ctgactggtg
     1081 accgtgatct tttgggcccg gaaggtttcc atgatgcgcg aagacacctt tttgttgcgg
     1141 gcgtacattt ttcgcaggtt cagcagaaac cccagccaac ctacgtcctt gatgatgatg
     1201 acgtcaaaca gaccatcgtt aaggctcgct tctggcgccg ggcacatccc gcggccaaag
     1261 tagcggccgt tggccacgat gaggttggtg agattgacac cggagatttg cgtgttgggt
     1321 agtcccgggc cggacaaatg gatggttgcc ggaatgttcc gatatccggc aaagccgcat
     1381 acggt
//
LOCUS       DATDUN010000456         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_605531, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000456 DATDUN010000000
VERSION     DATDUN010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605531"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VJC18_06385"
     CDS             complement(<1..858)
                     /locus_tag="VJC18_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45046.1"
                     /translation="MKYNPFNPNSVVSTNLFAGRKRYIFQMIRKLEQVKMGMPSSFFL
                     FGERGIGKTALAKLLKHIAESKDTMFGNLNFLTSYYTAEKGQTIGSVLQSSLNELTDR
                     LPKTALEVLSTKLGAVFKNGKFSIGPFSLEMASQPTPEVVTILKDQIVSILSNIFISL
                     KSSENQTNEKDGVLIIIDEMQNVSDLSLCAQLLRGIITTLDVKEMGLISFLLIGYDDA
                     AFFEGDLSARRQFDTICLEVMSLEEAGEAMTKGFQAAGVGHDEHELRKNIIATGGYPH
                     AIQLLAHNLL"
     gene            1089..>2166
                     /locus_tag="VJC18_06390"
     CDS             1089..>2166
                     /locus_tag="VJC18_06390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD45047.1"
                     /translation="MVSKIINLMKTAPAIERLTADKEELARLYRYWRIRAFYAVFFGY
                     AFFYVCRSNLSVALPRLITDLGYTKTQVGIIGSTFYATYSIAKFVNGVLCDKANPRYF
                     MGFGLLLAALANIAFAFGAKISFSLSATLGIVISPIFFFALFWLVNGWAQSMGSPMGP
                     KVMSSWFSISERGTKYSLYNTCHNVGAFVALAGGGWIVQNWGWQAGFILPGAFCALGA
                     FFIINRLPDRPESLGLAPISEYRNDFAVSENVADKITDQSTLRTIAWHYVFSNYRLWL
                     LAFASLFVYIIRYGLMDWAPTYLVQVKQSSIAMAGLKTSTLELMGIPGGILAGMATDY
                     FFKGRRIPVAIICLLLLALFATIFY"
BASE COUNT          546 a          523 c          456 g          641 t
ORIGIN      
        1 taaaagattg tgtgccaaca attgaattgc gtgtggatat cctcccgtcg caatgatatt
       61 ttttctgagt tcgtgttcat cgtgcccaac accagccgcc tgaaaacctt tcgtcatggc
      121 ttcgccagcc tcctccagag acatcacttc aagacagatt gtgtcgaatt gtctcctggc
      181 cgacaaatca ccttcaaaaa atgctgcgtc gtcatagcca atcaacaaaa acgatatcag
      241 acccatctct ttaacatcca gcgttgtaat gatgccccgc agaagttgtg cacacaaaga
      301 taggtcagag acattctgca tttcatctat gatgatcaac acgccatctt tttcgttcgt
      361 ttgattttcg gatgatttta acgaaataaa aatgtttgag agaatggaaa cgatttgatc
      421 tttgagaatt gttaccactt cgggagttgg ttgagaagcc atctctagag aaaaaggacc
      481 aattgaaaat ttgccgtttt tgaaaactgc tcccaatttt gtgcttaaaa cttccaaggc
      541 cgttttgggt agtctgtcag tcagctcatt caatgatgat tgaagaacac tgccaatcgt
      601 ttgccccttc tctgcagtat aatatgatgt cagaaaattt aagtttccaa acatggtgtc
      661 tttactttcc gcaatgtgtt ttagcaattt tgcaagagca gtttttccta tacctcgttc
      721 accaaaaaga aaaaaacttg atggcattcc cattttgacc tgttcaagtt ttctaatcat
      781 ttgaaaaata tatctttttc tgccagcaaa caaatttgtt gaaacaacag agttcggatt
      841 aaatggatta tatttcatct atagattcct catagtaact aagaaatatt catagtacaa
      901 ccatatgaaa tgtcaaatat gcatatattc tgcatatgaa aaagattgtc agtcacaaaa
      961 ggactgtttt gaaccaacac agcattggca agataattac aatgttttta cgttaataca
     1021 ttgatattct atgatatttt cgtatagtgc tcattggtgg cgaaacaaac caaaaaagga
     1081 ggggtctgtt ggtttccaaa atcatcaatc tcatgaaaac agcccccgcg attgagcgct
     1141 tgacggcaga taaagaggaa ctggcgcggc tttaccgtta ctggcgcata cgcgcattct
     1201 acgccgtgtt ctttgggtac gcatttttct atgtctgccg ctccaattta tccgtggccc
     1261 tgccgcgact catcactgat ttgggctaca ccaaaacaca ggtaggcatc attggctcca
     1321 ctttttacgc aacctattcc atcgccaagt tcgtaaatgg agtgctctgc gacaaggcca
     1381 atccccgcta cttcatgggc tttggcttat tgttggccgc gttggccaat atcgcctttg
     1441 cctttggggc aaaaatatct ttttcgctca gtgcgaccct cggcattgtg atcagcccca
     1501 ttttcttttt tgccctgttc tggttggtca atggttgggc ccagtccatg ggaagcccca
     1561 tgggccccaa ggtcatgtcg agttggtttt ccatctccga acgtggcacc aaatattccc
     1621 tttataacac ctgccacaat gtcggggcct ttgtggccct ggcgggcggc ggttggatcg
     1681 tgcaaaactg gggttggcag gcgggcttca tcctccccgg ggctttctgt gccttggggg
     1741 cttttttcat catcaaccgc ctgcccgatc gcccggaatc gctgggcttg gcaccgatca
     1801 gtgaataccg caacgacttc gccgtgtctg aaaatgttgc tgacaaaatc acggatcaat
     1861 ccaccctgcg cacaatcgcc tggcattatg ttttttccaa ttaccgattg tggttgctcg
     1921 ccttcgcctc cctgttcgtg tacatcatcc gttacggatt gatggactgg gcaccaacat
     1981 acctcgttca agtcaaacaa agctcgatcg ccatggcggg actaaaaacc tccacgcttg
     2041 aattgatggg catccccggc gggatcctcg cgggtatggc cacagattat ttttttaaag
     2101 gccgcaggat ccctgtggcc atcatctgcc ttttgctgtt ggccctgttc gcgacaatat
     2161 tttatt
//
LOCUS       DATDUN010000457         3280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_147333, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000457 DATDUN010000000
VERSION     DATDUN010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147333"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2155
                     /locus_tag="VJC18_06395"
     CDS             <1..2155
                     /locus_tag="VJC18_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLD45048.1"
                     /translation="DEKAHKNLFPMTNSLNQDDLELITEESDRYQATHEAIVCEAQKK
                     GRQYEENEALARELTSQIVTTHRDEDKQALQSDESVAHGLTKLRLEQNRSLKLLLEQP
                     YFARVVYCESDKSTEFKLGLASFPEQRIIDWRKAPLSKLYYDYEEGQSFDTEIAGRER
                     QGSIELRRAYQGKRNTLSQIDLNDRSYVLSRGQWSRYQRIKAGTFSLKDKEKIRELLK
                     NQDRLATAQPTEETGYLGQILSLLSPEQFCLISTETHKPVIIQGSAGTGKTTVALHRL
                     AWLLYEGNSAARPEQSIVLVYNKSLAAFVGHVLPELGVDKVKVATYHDWALDIVNTTQ
                     NLTLNHPVTDVPEEIVAHKTGLGLVDEIVAFCVSSKNSESAVDVLMAYYGCVLERHQK
                     REIDLGSPLVHYLSEQVLKQRLDVCDLALLLHIIHVRNQAYRATKHPTSLDYLVVDEA
                     QDFSPGELKTIFYALSDMNNLTMAGDLGQKILSQNRAGDWETLLSQSGLTGIDVLNLR
                     VAYRSTYQIYEMAEFIRDPGLSDEELKLKPKFGPEPVLTKCHAFLDAIDCVANWLDSV
                     LVENRATIGAIICKTGVEARQVYDALIKQGVRGIRLGDMTHFEFTPGITITDIRQVKG
                     LEFSTVMLFNPSQKNYSIHSMLDRNLLYVAVTRASFRLDMVCHDTPSDLIPHFIYVND
                     LTAIAENFDDKPLFSNVDQDVGEEEQTGDDARDED"
     gene            2239..3135
                     /locus_tag="VJC18_06400"
     CDS             2239..3135
                     /locus_tag="VJC18_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLD45049.1"
                     /translation="MEKGHDQKALLFVDIVDSTIYYDTLGDEKAQTLMADAMSTLSDI
                     VMQYGGEVVKTMGDAVMATFDSAEVAAKSALFMQRIFQTNAVSVLAQPGELKVRVGIN
                     FGDVVVDKDDVFGNAVIVASRVASIAKPEQILTTRDTRDLLPKELIKASRFILKKQLK
                     GLPEEHEIFEILNAPDDANVTTIDSGGTTSLTEELSLILSHGDQKIKLDQNRTVVSIG
                     RNEENDLVIPSTKVSRHHAKIELKNGRYVFTDKSANGSYIAQEGEPLIKVHVDQTTLY
                     QSGKIYLGGRDDESCLVCFSHQ"
     gene            complement(3129..>3280)
                     /locus_tag="VJC18_06405"
     CDS             complement(3129..>3280)
                     /locus_tag="VJC18_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HLD45050.1"
                     /translation="TNPANAAPGTIRQLYAESLQCNSVHGSDAPETAKVEIGFFFDDQ
                     SLFDY"
BASE COUNT          868 a          759 c          888 g          765 t
ORIGIN      
        1 tgatgagaag gcgcataaaa atctattccc catgactaat tcgttaaatc aggacgacct
       61 cgaactcatt accgaagaaa gtgatcgcta tcaagcaact catgaggcca tcgtttgcga
      121 agcgcagaag aaggggcgtc agtatgagga aaatgaggcg ttggcgcggg agctcaccag
      181 ccaaatcgtg accacgcacc gcgacgaaga caaacaggcc cttcagagtg acgaaagcgt
      241 agctcatggc ttgaccaaac tcagactgga gcagaatcgt tcacttaaac ttctgttgga
      301 acaaccctac tttgcgcggg tcgtgtattg cgaaagtgac aagagtacgg aattcaagct
      361 ggggcttgcc agttttcccg aacaacgcat catcgattgg cgcaaggcgc ccctttccaa
      421 actctattac gattatgagg aagggcagag cttcgatacc gaaattgccg gcagggaaag
      481 gcagggctct attgagctca gacgcgctta tcagggcaaa agaaataccc tgtcgcaaat
      541 tgatctcaac gatcggagtt atgtgctcag cagggggcag tggtcacgtt atcaaagaat
      601 caaggcaggg accttcagcc tgaaggacaa ggaaaaaatc agggaactgc taaaaaatca
      661 ggatcgtttg gccacggcac aaccaactga agaaacgggt tatttgggcc agatattatc
      721 gttgctgtca cccgaacaat tttgtctgat cagtaccgaa acgcacaaac ccgtgatcat
      781 acagggttca gcaggaactg gcaaaaccac ggtggccttg catcgtctgg cgtggctttt
      841 gtatgaaggg aattcagcgg cgcgtcccga acaatccatc gttctggttt ataacaagag
      901 cctggcggcg ttcgtagggc acgtcctgcc tgagctgggt gttgataagg tgaaggtggc
      961 gacttatcat gattgggcgc tcgacatcgt caacaccacg cagaatctga ctttgaatca
     1021 ccctgtcact gatgtgccgg aagagatcgt ggcgcacaaa accggtctgg ggctggttga
     1081 tgagatcgtg gcattttgtg tcagtagcaa aaacagtgag tccgctgtgg atgtgttgat
     1141 ggcgtactat gggtgcgtgc tggagcgaca ccaaaaaaga gagatcgatt tgggatcacc
     1201 gttggtgcat tatctgagtg aacaggtttt gaagcaaagg cttgatgttt gtgatctggc
     1261 cctcttgttg cacatcattc atgtcaggaa ccaggcctac cgcgcgacga aacacccgac
     1321 aagtctggat tatctggtgg ttgatgaagc tcaggacttc agtcccggtg agctcaaaac
     1381 cattttttac gcgctgtccg atatgaataa tctgacgatg gcgggtgatt tggggcaaaa
     1441 aatattgtct caaaatcggg ctggggactg ggaaactttg ctgagtcaat cagggttgac
     1501 agggatcgat gtacttaatt tgcgggtggc ttacaggtcc acataccaga tttacgaaat
     1561 ggcggagttt atccgcgatc ccggtttgtc cgatgaagaa ctgaaactca aaccaaaatt
     1621 tggtcctgaa ccagttttga ccaaatgcca tgcttttttg gatgccattg attgtgtggc
     1681 aaattggttg gattctgtgc ttgtcgaaaa ccgcgcaacc atcggggcta tcatttgcaa
     1741 gacaggtgtg gaggccagac aggtgtatga cgcgctcatc aaacaagggg tcagggggat
     1801 tcgtctggga gacatgaccc attttgagtt cacaccgggg atcaccatca ccgacatcag
     1861 acaagtcaag gggctggaat tttcaacggt catgttgttc aacccctctc aaaagaacta
     1921 ttcgattcac tcgatgctgg atcgaaatct cctgtatgtg gctgtcacgc gcgcttcatt
     1981 caggctcgac atggtatgcc atgatacacc gagtgatttg atcccccact ttatctatgt
     2041 gaatgacctg acggccatag ctgagaattt cgatgacaaa ccactattct cgaatgtgga
     2101 tcaggatgtg ggggaggaag agcaaactgg cgatgatgct cgtgatgaag attgatatag
     2161 gtcactgacg ggtcactgat acagggatat ttttcttctt tttttctcca atttgctcta
     2221 tgtgactggg tgagaaatat ggaaaaaggt cacgatcaaa aagccctcct ctttgtcgac
     2281 atcgtggata gcaccatcta ttatgatacc ctgggcgacg aaaaagcgca gacgctgatg
     2341 gccgatgcca tgagcacgct gagtgatatt gtcatgcaat atggcggcga ggtggtcaaa
     2401 accatgggag acgcggtgat ggccaccttc gacagcgccg aggtggcggc caagtctgcc
     2461 ttgttcatgc aacgcatctt tcagaccaat gccgtttcgg tcctggcgca gccgggagag
     2521 cttaaggtca gggtggggat caattttggt gatgtcgtgg tggataaaga cgacgtgttt
     2581 ggtaacgccg tgattgtggc gtctcgtgtg gcatccatcg ccaaacccga acagatcctc
     2641 acgaccaggg acacacgcga tctgttgccc aaagaactca tcaaggcgtc gcgctttatt
     2701 ctcaaaaaac aactcaaggg gctccctgaa gaacacgaga ttttcgaaat cctcaacgcg
     2761 ccggatgatg ccaacgtgac gacgatcgac agcggtggga ccacgagttt aaccgaagaa
     2821 ctgagtctca tcctgtcgca cggtgatcag aagatcaaac ttgatcagaa ccggacggtc
     2881 gtttccatag ggcgcaacga ggaaaatgat ctggtgattc ccagcaccaa ggtctctcgc
     2941 catcacgcca agatagaact caaaaatggc cgctatgtct ttaccgacaa aagcgccaat
     3001 ggttcataca ttgcacagga aggtgagcct ctcatcaagg tacacgtcga ccaaacaacc
     3061 ctctaccaga gtggtaaaat ctatttaggc ggacgcgatg atgaatcttg cctggtctgt
     3121 ttctcgcatc aataatcaaa caaactttga tcatcgaaga agaaaccaat ctccactttg
     3181 gccgtttcgg gggcgtccga accatgcacg gaattgcatt gcaggctttc ggcgtagagc
     3241 tggcggatgg tgccgggagc ggcattggcg ggattggtcg
//
LOCUS       DATDUN010000458         1451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_312252, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000458 DATDUN010000000
VERSION     DATDUN010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312252"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VJC18_06410"
     CDS             <1..360
                     /locus_tag="VJC18_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45051.1"
                     /translation="KHAWEVATSSPIAYRDVAVASKEAVAALDESFFRVRFDRLTPSE
                     KKYMRAMAEIGPGPHRSGDIAEKLGRQVTSLGPTRSQLIAKGMIWSPNHGDTAFTVPL
                     FDEFMCRIMPGNDWKEE"
     gene            complement(429..1190)
                     /locus_tag="VJC18_06415"
     CDS             complement(429..1190)
                     /locus_tag="VJC18_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45052.1"
                     /translation="MIGLKAIFYKKRKGAKMYLSPISSFCQMMPTIPTRGSIRSDDFL
                     VQADPKWLAPPPLECSLSSDRGLFHIQTNRPDSTPPKMHHLLLGEDPRYWDETYREQW
                     MNEGHSNAKLIPGEACLHRKSNSVLPTFNFHSMDRQTHPIIFTLQHNPLGSAYELTAN
                     DGIRYNLIPATLAIMLEGAEHLFYGRFQIRDGAEQRRRFTEVIPLEHQDRSWSNSVTD
                     AEWYYAVVSEITNDTAGRWVHKFVFPNSPPYPVAT"
BASE COUNT          389 a          331 c          354 g          377 t
ORIGIN      
        1 aaacacgctt gggaagtcgc tacatcctca cctatcgcgt acagagatgt tgcggtggcc
       61 tcaaaagagg ccgtggcagc attggacgaa agttttttca gagtgcgatt cgatcgactg
      121 accccttccg aaaaaaaata catgcgggcc atggcagaaa ttggtcctgg tccgcatcgt
      181 tctggcgaca tcgccgaaaa gcttggtcgt caggtcacat cattgggacc cacacgaagt
      241 cagttgatcg ccaaggggat gatctggagt ccgaaccatg gggatacagc cttcacggtt
      301 cctctctttg atgaattcat gtgccgaatt atgcctggca atgattggaa agaggaatag
      361 gaataatgtg cccggcacct gatttctgat ttttgagaac aatttgaatt tgaacaatcg
      421 aacttgaatt atgtggcgac aggatagggg ggagagttag gaaagacgaa cttgtgcacc
      481 catctgccag cagtgtcatt ggttatctcg ctgacaacgg cgtaatacca ctccgcatca
      541 gtaacactgt ttgaccacga acgatcctga tgttctaatg gaatgacttc agtaaatctt
      601 ctgcgttgtt ctgcgccatc acgtatctga aaccgtccat aaaataaatg ctctgcgcct
      661 tcgagcatga tagccagagt ggcagggatc aaattatagc ggatgccatc attagctgtc
      721 agctcgtagg cacttcccaa cggattatgt tgtagagtga aaataatggg gtgagtctgt
      781 ctatccatgg aatgaaagtt aaatgtgggc aatacgctat ttgatttccg gtgcaaacag
      841 gcttcacctg gtattaattt ggcattagaa tgtccttcat tcatccattg ttcgcggtat
      901 gtttcgtccc aatatctggg gtcttcaccc aacagaagat ggtgcatttt gggcggcgtg
      961 gagtcaggtc ggttggtttg tatgtggaac aatcccctgt cagaagacaa actacattct
     1021 aagggaggcg gggccagcca tttgggatca gcctgcacca gaaaatcatc agaacgaatt
     1081 gatccacggg ttggtattgt cggcatcatt tggcagaaag aagatatggg tgaaagatac
     1141 atttttgctc ccttccgttt tttgtaaaaa attgccttca aaccaatcat cggcaaagcg
     1201 cagcaaaggt tgtgttgtgc cccaaatctc cgtgtcccag cggacacggt ttttgtgcgg
     1261 gtgttaagtc ttataatgat ctttgccagt gattgttgat aatccggcac cgactggcaa
     1321 caccaatcat tcaaggagac aaccatgact gaacccatca acaaccaaac cgaaaagacc
     1381 acactacttt acgatgaacc actgttggct gacatcacac tctcagaacc caccgacctg
     1441 tcggctgttc t
//
LOCUS       DATDUN010000459         1535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_271935, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000459 DATDUN010000000
VERSION     DATDUN010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271935"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VJC18_06420"
     CDS             <1..575
                     /locus_tag="VJC18_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HLD45053.1"
                     /translation="PLPTHWRVVFPLAIALFFIAFAVLVAFQKKGMFSTLSRLVARAG
                     LQKERIGKYADRILALDKQIAVFYGKNKLHFFEIMMLQLVGRLLGAVEIYIIAWILGM
                     PLEISHCLYLTSLTVLINVVFVFIPGSMGVMESGYGALFHLLKINFEYGVALQLVRRI
                     RTFFWIGIGLMIILFYKPKKGISSKLKGQR"
     gene            744..>1535
                     /locus_tag="VJC18_06425"
     CDS             744..>1535
                     /locus_tag="VJC18_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45054.1"
                     /translation="MQYSKLMRFLFTNAFVKLLIILRLPLTKLLLSSSDRLSRYENIV
                     KSQTISGMVPEFNKLQQFLFWPFYRRVQFLPADVQTLKAMTDPVVYVIKNRGQLEYQY
                     FNYLFLKEKIALVHFAKGLCTLPWLPLKAMGKHLIVHLYHFYRDYDEGVLLQDKRKMM
                     ALLTANKSVMLSLRISRDYLFGLVKTDPANDLIPLVEIQKTIAQSISVVTVQFLYDKH
                     PDKSELSYFDLLFGEKSQPGVIRKFILFLLNYRRHPRVKFGVPVEL"
BASE COUNT          377 a          346 c          357 g          455 t
ORIGIN      
        1 tgcccctgcc cacacattgg cgtgtggttt tccctcttgc catagctctc ttttttattg
       61 cctttgcggt tttggtggcg tttcaaaaaa aaggcatgtt cagtaccttg agtcgtctgg
      121 tagcgagggc gggtttacag aaagagcgga tcggaaaata tgctgaccgc attttggcac
      181 tcgataaaca aatcgctgtt ttttacggca agaacaaact ccattttttt gagatcatga
      241 tgttgcaact ggtgggaaga ctgcttggtg ctgtcgaaat atatatcatt gcctggattc
      301 tgggtatgcc gttggaaatc agtcattgcc tctacctcac ttctttgacg gttctcatca
      361 atgtcgtatt tgtcttcatt cccggcagta tgggggtgat ggagagtggg tacggagctc
      421 tttttcatct cctgaaaatc aattttgaat acggcgtggc cctccagctc gtgcgtcgca
      481 tccgcacttt tttctggatc ggcattgggc tgatgatcat tctgttttat aaaccgaaga
      541 aagggataag ctcaaagctg aaaggtcaaa gatgaaaggg taatggattt ccgctaacag
      601 ctcacagcca acagctgacg gccaaagcta atataatggc cgtgggcagt cgaccgtaag
      661 ccgtcggcag aatattttgc tttgagcttt gagcttgtac ctctcagctt tactcgtaaa
      721 ccccacttgt ctattacagc ctcgtgcagt atagtaagct tatgaggttt ctgttcacaa
      781 atgcctttgt taagctgttg attattctgc ggttgccgtt aaccaagctg ttactctcga
      841 gttccgatcg cttgagccga tacgaaaaca tagtcaaaag ccagacgatt tctggcatgg
      901 ttcccgaatt caacaagctg caacaattcc tgttttggcc cttttatcgt cgtgtgcaat
      961 ttttgcctgc ggacgtgcaa accctcaaag ccatgacaga tcccgtggta tatgtgatca
     1021 aaaaccgggg acagctcgaa taccaatatt tcaattatct ctttctcaaa gaaaaaatcg
     1081 ccctggttca ttttgccaag gggttgtgta cgctcccctg gttgcctttg aaggccatgg
     1141 ggaagcacct gatagttcat ctctatcact tctatcgcga ttacgatgag ggtgtgcttt
     1201 tgcaggacaa acgtaaaatg atggcactgc tcacagccaa caaaagcgtg atgttgagct
     1261 tgagaatttc tcgggattat ctgtttgggt tggtgaagac agatcccgcg aacgatttga
     1321 tcccgcttgt cgaaatccaa aaaaccatcg cacaatcgat cagcgttgtg actgttcagt
     1381 tcctctacga caaacacccc gacaaatcag agttgtcata ttttgacttg ttatttggag
     1441 aaaaatcaca gcctggtgtc atcagaaaat tcatcctttt tctgctgaat taccgacggc
     1501 accccagggt gaagtttggt gttccggtag agtta
//
LOCUS       DATDUN010000460         1852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_395696, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000460 DATDUN010000000
VERSION     DATDUN010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395696"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..>1852)
                     /locus_tag="VJC18_06430"
     CDS             complement(366..>1852)
                     /locus_tag="VJC18_06430"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HLD45055.1"
                     /translation="LHNLESIQTYFSSELVLFLGIVLLLWASVNKKQDRARTCAILAV
                     SVLAFSLVAGVFTHFSQELLLFHGLVALDAFGQFFKIIITFTAIIVVVLATRSNDLDG
                     SPKPEFYALLITLTLGLTILSTANHLLMIYLAVEMSSLVSYVLAGYITENTRSDEAGL
                     KYVLYGGVASAVMLFGMSLLYGVTGEMGLVSIREFLISHPVDPMILFLAVIMIFAGLG
                     YKMAVVPFHMWSPDVYEGAPLPVTAYLSVASKAGGFAVTLRFFLVGFVGASSKDLWMA
                     LGGIDWPLLIALLSATTMTVGNLLALHQQNIKRFLAYSSIAHAGYLLMGLVAVSRAGV
                     EAVLYYFAIYFLMNLGAFVVAQVLVNQGKTENMEDYRGLVRQGPFGVFLSLSLTVFLF
                     SLAGIPPFAGFVAKWYVFKAVIDSGYLWLAIVAVINSVVALYYYVRLIKFMLIDETQT
                     VLVLKNTHWRYAGIIFIFASLVLLFGVYFSPIVKWAQVSAVMYY"
BASE COUNT          564 a          558 c          417 g          313 t
ORIGIN      
        1 ctctcgcaag ccgagaggcg aaagaagaca aggccattga aaagtggttg cgactggccg
       61 gcaatgggtc ttacagcacc tcatcccgcg aggatcaact gcaatacttg ttgaaggacg
      121 caacaggaaa cgaggaagcg aaagcaattg tggacgaaat gaattcgtaa agaattaaac
      181 acgtggattg ccacgcttca cttcgtgaag ctcctgcctg acggcaggca cggcggcaac
      241 actgccctcc ccaacgtcaa cacgtacgca cgtcaacacg tacgcacgct ttccccatca
      301 cccataaccc ataaccacat acccatcttc cgccttacgc cttaagccct acgccttgcg
      361 cctttttaat aatacatcac cgcgctcacc tgcgcccact tgacgatcgg ggaaaaataa
      421 acgccaaaca acaacaccag cgaggcaaaa ataaagatga tgcccgcgta acgccaatgc
      481 gtgtttttga gcaccagcac agtttgtgtc tcatcgatca acatgaactt gatgagccgc
      541 acataataat acagggccac aacggaattg atgaccgcca caatcgccaa ccagaggtag
      601 cccgaatcaa tgacggcctt aaaaacatac cacttggcca caaagccagc gaacggtggg
      661 atccctgcga gtgaaaataa aaaaaccgtc aggctcagag acaaaaacac accgaacggt
      721 ccctgccgca ccaaccccct gtagtcctcc atgttctcag tcttgccctg attcaccaac
      781 acctgcgcca ccacgaaggc ccccagattc atcaaaaaat agatcgcgaa ataatacaac
      841 acggcctcca cgcccgcgcg cgacacggcg acaaggccca tcaacaaata ccccgcatgc
      901 gcgatcgagg aataagccag gaaccgtttg atgttttgct ggtgcagggc caacagattt
      961 cccaccgtca tggtcgtggc agacaacaaa gcgatcagta aaggccaatc aatcccgccc
     1021 agggccatcc acaggtcttt ggacgacgcc cccacaaacc ccaccagaaa aaatctgagc
     1081 gtaacggcaa agccccctgc cttgctggcc acactcaaat aagccgtcac cggcaatggg
     1141 gccccctcat aaacatcggg cgaccacatg tgaaacggca ccacagccat cttgtagccc
     1201 aaaccggcaa agatcatgat gacggctaaa aacaaaatca tcggatcgac cgggtgactg
     1261 atgagaaatt cacgaatcga taccagaccc atctcccctg tgacgccgta caaaagcgac
     1321 atcccaaaga gcatcacagc gctggccacc cctccataca acacgtactt cagccccgcc
     1381 tcatcggaac gggtattttc tgtaatgtaa cctgccagca cgtaagagac cagtgacgac
     1441 atctcaaccg cgagataaat catcaacagg tggttggccg tcgacaggat ggtcagaccc
     1501 agcgtgagtg tgatgagcag ggcataaaat tcaggcttgg gagatccgtc cagatcgttt
     1561 gacctcgtcg ccagaacgac gacaatgatc gccgtaaacg tgatgatgat tttaaaaaac
     1621 tggccaaagg catctagggc gaccagacca tgaaacagca acagttcttg cgaaaaatgc
     1681 gtgaagacac cggccacgag cgagaaagcc agcacggaca cagccaggat cgcgcaggtc
     1741 cgcgcacgat cctgcttttt gttgaccgac gcccagagca acagaacaat ccccagaaac
     1801 aacacgagct ctgaggaaaa ataggtttgt atggattcca gattatgcaa ca
//
LOCUS       DATDUN010000461         1535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_312282, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000461 DATDUN010000000
VERSION     DATDUN010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312282"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VJC18_06435"
     CDS             <1..421
                     /locus_tag="VJC18_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HLD45056.1"
                     /translation="LSNGPGDPAACTEAIGNVKKLLGKVPLFGICLGHQILALALGAK
                     TYKLKFGHHGGNQPVKNLKTGAVEITSQNHGFAVDPHSLPLGTEVTHVHLNDNTISGI
                     AHEELAVFSVQYHPEASPGPHDSRYLFEQFCGMMRTR"
     gene            644..>1535
                     /gene="carB"
                     /locus_tag="VJC18_06440"
     CDS             644..>1535
                     /gene="carB"
                     /locus_tag="VJC18_06440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546879.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HLD45057.1"
                     /translation="MPKRTDIKTILIIGAGPIIIGQACEFDYSGTQGVKALKEEGYRI
                     VLINSNPATIMTDPELADRTYIEPITVEIIEQIIQKERPDALLPTLGGQTALNVAVAL
                     DEAGVLKKYNVELIGARLSAITKAEDRLLFREAMKNIGLNVPKSVVVSSVEEAGREIK
                     DIGFPAILRPAFTLGGMGGAVSYHEKEFKDQVLWALDCSPVKQVLIEQSLLGWKEYEL
                     EVVRDQKDNVIIICSIENVDPMGIHTGDSITVAPAQTLTDREYQSLRNQAIAVIREIG
                     VDTGGSNIQFAVNPKNGDVMV"
BASE COUNT          393 a          353 c          429 g          360 t
ORIGIN      
        1 cctgtcaaat ggccccggtg atcctgcggc gtgcacagag gccattggga acgttaaaaa
       61 acttctggga aaagttccct tgtttggcat ctgtcttggt caccagatac tggctttggc
      121 cttgggggcc aaaacctaca agctgaaatt tggtcatcac gggggcaatc agccggtaaa
      181 gaatctcaag acaggcgccg ttgagatcac atcacaaaat catggttttg cggttgatcc
      241 ccattctttg cccctgggga cagaagtgac gcatgtgcac ctcaatgaca ataccatttc
      301 ggggattgca catgaggaac tggcggtctt ttcagtacaa tatcaccccg aggcgtcacc
      361 gggtccgcat gatagtcggt atttgtttga acagttttgt gggatgatga gaacacgata
      421 gaacgtgttt acagaagggg cgcaaggtat aagggtgttg ggcgttgtca tcccagcgtc
      481 ccgcaaacgg gatcagtcct aaggctgaaa ggctgggatc catgaagaat tgttgaaaaa
      541 atggattcct gcctacgcag gaatgacaat gagaactgag gtgatagtat tcacgtgtac
      601 atggtttcga tccacgatca acgatcgtcg atcctcgatt catatgccca aacgaactga
      661 cataaaaacc atcctcatca tcggtgcggg tccgatcatc atcggccagg catgtgaatt
      721 tgattattct ggcacgcaag gggtcaaggc cctcaaggaa gaagggtacc gtattgtgct
      781 catcaactcc aaccccgcga ccatcatgac agatccagag ttggcggaca ggacttacat
      841 tgaacccatt actgtcgaga tcatcgagca gatcattcaa aaagagcggc ccgacgcgct
      901 tcttcccacc ctgggtgggc agacggcctt gaacgtcgct gtggcgctgg atgaggccgg
      961 ggttcttaaa aaatacaatg ttgaactcat tggtgccagg ctttctgcca taacaaaggc
     1021 cgaagatcgt ctgctgtttc gtgaggccat gaaaaatatc gggcttaacg tgccaaagtc
     1081 tgtggttgtg agttctgttg aagaggccgg gcgggagatt aaggacattg gctttcccgc
     1141 tatcctgaga cccgcgttca cattgggcgg aatgggaggg gctgtttcgt atcatgaaaa
     1201 agaattcaag gaccaggttt tgtgggcgct ggattgttcg ccagtcaagc aggtgctcat
     1261 cgaacaatcg ctattgggtt ggaaggaata tgaactcgaa gtggtgcgcg atcagaaaga
     1321 taatgtgatc atcatctgct cgatcgagaa tgtggacccc atgggcatcc acacgggtga
     1381 ctccatcacg gtggcaccag cgcaaacgct cacggacagg gagtatcagt ctttacgcaa
     1441 tcaggcgatt gccgtgatcc gtgaaattgg cgtggacaca ggggggtcga atatccagtt
     1501 tgctgtgaat cccaaaaatg gcgatgtcat ggtga
//
LOCUS       DATDUN010000462         4258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_312307, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000462 DATDUN010000000
VERSION     DATDUN010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312307"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..794)
                     /locus_tag="VJC18_06445"
     CDS             complement(150..794)
                     /locus_tag="VJC18_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLD45058.1"
                     /translation="MFDIFGHAAFLLMAVSFMVKDIFWLRVMSIGASVCSIVYNFTAL
                     QDPIWLVIQWNMIFILINLYRGAILIQDKMHVSITEDEQELYETQFRNFSPVEFMKIL
                     RAATWHDVDANEILAKEGKPISYLMVIYCGKADVVVKGHIVAQLKDGDFIGEMSFNTD
                     KLAAATVAAAEPMRLLVWNKTQLKDMLRRNPSIHFAMQSILSANLVDKLARVPN"
     gene            complement(823..1785)
                     /locus_tag="VJC18_06450"
     CDS             complement(823..1785)
                     /locus_tag="VJC18_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45059.1"
                     /translation="MKISGIFFVGLVSILFCPLIVNANLPENTITFAQGHKKNVPLVF
                     MNATHCHKSPCVIGQYGAIKFAPGLSATHDIKLSYLHDDQMMSFSLFQEGALFKEGLF
                     DRLVNKQRNRNVFNLEQSLAAGQTDTVYLYGVLLHYQLEEGDLIVIDIINHKDAKDKH
                     QCIFRFHRTGPIFDADVALINPIDYFAPNPNDTVKYASISIAVSTSVGWYMNPEKDYN
                     FAQKFLGAWRFNIFTGLLRRHELTNIGGDNIVDARFDGFVGAGFTFVDFFIGGMGVNL
                     VRSPHTVFPFVGVEVKHLYQFMKSIKKSTKKRWNKYQELVMSNE"
     gene            1933..2586
                     /locus_tag="VJC18_06455"
     CDS             1933..2586
                     /locus_tag="VJC18_06455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HLD45060.1"
                     /translation="MVIERLKYIAVDGPIGVGKTSLANMLAQDLGARAVYENPDENPF
                     IGNFYEDANRYAFQTQLFFLLSRFRQQTELIQQELFEQKVVCDYVFAKDLVFARLNLS
                     DEEFSLYAQVYNLLDQRLPKPDVVIFLQASPDILMKRIRKRCKDYEKQIEPDYIVRVA
                     QAYSQFYFQYTDSPLLVVNTSGLDFVKHEIDYEMLKKELVALISSGKDKHYVTISPR"
     gene            2759..3610
                     /gene="panB"
                     /locus_tag="VJC18_06460"
     CDS             2759..3610
                     /gene="panB"
                     /locus_tag="VJC18_06460"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HLD45061.1"
                     /translation="MSSQKKNSGSKMTISDLQSLYSREEKITVLTAYDYTMARMADDA
                     GVDIVLVGDSLGMVIQGEDTTLPVDIEHMVYHTRCVAKGIKRAHLMADMPFMSYQPSL
                     DVAVSNAGSLIKAGAASVKLEGGEEMADLVWYLDSIGIPVCAHIGLKPQSIHKLGGYK
                     VQGRSKLQAEQIVADAKILEEAGAFSLLLEGIPLEVAREITQTVAIPTIGIGSGPYCS
                     GQVLVCYDVLGADPDFEPRFVKKYLNLHEQVTQAFSAFAAEVKAGQFPSDKHCFHQNV
                     VAVKAIK"
     gene            complement(3637..>4258)
                     /locus_tag="VJC18_06465"
     CDS             complement(3637..>4258)
                     /locus_tag="VJC18_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45062.1"
                     /translation="PYLLSPLKRFHGLINLASWLLVFAYTLTIGLVLYGHFMPGFDSS
                     FITKRFAQQGYLSQHDQDRHKTRPKKSLPKEEPAVDPVAEPHQIRETETPNSANEIPA
                     ETAPENNQPVSPSALPALETVVPPNTAVEIQSVPVSEPQPVAGTLPDSGAAYFEPSPA
                     VGNDVSRLAEEKAQLQNKYNDLLRKNIELEKEIEDLKNLIHSQENH"
BASE COUNT         1064 a          951 c         1132 g         1111 t
ORIGIN      
        1 gttgaggcgg tgacgctttt tgactgcaaa cgcacacttg aagaagatgg tattctgaaa
       61 aacgccatca tgaccagtcg cgataaaatc acacccgatg tgatcgagcg actcaggccg
      121 attgtgggga aatattatca aactttcagc tagtttggca cccgtgccaa tttatccacc
      181 agattcgcgc ttaaaatcga ttgcatcgca aaatggatgc tggggtttct tcttaacatg
      241 tccttgagtt gtgtcttgtt ccaaacgagc aggcgcatgg gttccgcggc ggcgacagtg
      301 gccgcggcca atttgtcggt gttgaagctc atctcgccaa taaagtcacc gtctttgagt
      361 tgtgccacga tgtgcccttt gaccaccaca tcggccttgc cgcaatagat caccatgagg
      421 taggaaatgg gtttgccttc cttggccaga atctcgttgg catcgacgtc gtgccaggta
      481 gccgcccgaa ggatcttcat gaactccacc ggagaaaaat tgcggaattg tgtttcgtac
      541 aactcctgtt catcctcagt aatagagacg tgcattttgt cctgaatgag aatggcgcct
      601 cgatagaggt tgatgaggat aaagatcatg ttccattgaa ttaccagcca gatgggatcc
      661 tgtagggcag taaagttata gacaatggag caaaccgagg ccccgatcga catcacgcgc
      721 aaccaaaaaa tgtctttgac catgaacgaa acagccatca gcaaaaatgc cgcatgtcca
      781 aagatatcaa acatagaata cccccccatc actcataact catcactcat tactcattac
      841 tagctcctga tacttgttcc acctcttctt ggtgcttttc ttgattgact tcatgaactg
      901 gtagaggtgt ttgacttcta ccccaacaaa gggaaacact gtatgtgggg agcgcaccag
      961 attgaccccc atcccgccaa taaagaaatc aacaaatgtg aaacccgcgc ccacaaagcc
     1021 gtcgaaacgg gcatccacaa tgttgtcgcc accaatgttg gtgagttcat gacgtctgag
     1081 caatcccgta aaaatattga atcgccaggc ccccaggaat ttttgcgcga aattgtaatc
     1141 cttttcgggg ttcatgtacc aacccacgga ggtggagaca gcgatggaga tggaggcgta
     1201 tttgaccgtg tcgttgggat tgggcgcgaa ataatcgatg gggttgatga gggccacgtc
     1261 ggcgtcaaaa atgggtccgg tacggtgaaa ccgaaagata cattggtgtt tatctttggc
     1321 atctttgtga ttgatgatgt cgattacgat gagatcgccc tcctccagct gataatgtag
     1381 caggacgccg taaagataaa cggtgtctgt ttgaccagcg gccagggatt gttccagatt
     1441 gaaaacattg cggttgcgtt gcttgttaac caaacgatcg aaaaggcctt ctttgaacag
     1501 ggcgccctct tgaaacaggg aaaaagacat catttggtca tcgtgcaggt agctcaattt
     1561 gatgtcgtgt gttgctgaaa gtccgggtgc gaacttgatg gcgccgtatt gaccgatgac
     1621 gcaaggggac ttgtgacaat gggttgcgtt catgaaaacg aggggcacat tttttttgtg
     1681 accttgagca aaggtgatgg tgttttcagg caggttggcg ttgacgatca aaggacaaaa
     1741 gaggatgctg acaagcccca caaagaatat ccctgatatt ttcatgaagc catggatagt
     1801 gcaatttggg cattaaagca actcctgaac cctgtattca ccagctcata atcctctatt
     1861 attccttgat atttatcttg tacaacacag cccttgccta ttctttggca attcaatatg
     1921 agcgggtgat ctatggtaat tgagcgcctc aaatacattg ccgttgatgg tcccattggt
     1981 gtggggaaga cgagtctggc caacatgttg gcccaagatc tgggtgcccg tgccgtttac
     2041 gagaaccccg atgagaatcc gtttattggc aatttttacg aggacgccaa ccgttacgcc
     2101 tttcagaccc agcttttctt tttgttgtcc cgttttcgcc agcagacgga gctgatccaa
     2161 caggaattat tcgaacaaaa agttgtgtgt gattatgttt ttgccaagga cctggtgttt
     2221 gcccgcctca atctgtcgga cgaagaattc tccctgtatg cccaggtcta taatttgctg
     2281 gatcaacgcc tgcccaaacc cgacgtggtg atttttttgc aagccagtcc cgatatcctc
     2341 atgaagcgta tacgtaaacg gtgcaaagat tacgaaaaac aaatcgaacc cgattacatt
     2401 gtgagagtgg cacaggccta cagccagttt tattttcaat acacggattc tccccttctg
     2461 gtggtgaata cgtcggggct tgattttgtg aaacacgaaa tagattacga aatgctcaag
     2521 aaagaactcg tggccctgat ttcctcgggc aaagacaaac actatgtgac catctcgccg
     2581 aggtaaaagt cgtgcgttcg tgcgtacgtg ttgacgaaag gggcgaaagg cgaaaggcgt
     2641 aaggcgtagg gctcaaggct aagggaaaaa cggtgaagat tgaatgtgct gttttatacc
     2701 ttacgcctta agccttgggc cttttgccct ttgtgaacac gtgaacaaga gagtagccat
     2761 gtcatcacaa aagaaaaatt ccggttccaa aatgaccatt agtgatttgc agtctcttta
     2821 cagccgagaa gaaaaaatca cggtcctcac tgcctatgat tacacgatgg cacgaatggc
     2881 cgatgatgcg ggtgtggata tcgttctggt gggagattct ttgggcatgg tgattcaagg
     2941 cgaagacaca accctgccgg tcgatattga acacatggtg tatcacacgc gctgtgtggc
     3001 caaaggaatc aagcgggcgc acctcatggc cgatatgccc ttcatgtcgt atcagcccag
     3061 tctggacgtt gccgtgagca acgcaggcag tctcatcaag gcaggggccg catcggtcaa
     3121 gctggagggt ggagaagaga tggccgatct ggtatggtat ctggattcga ttggcatccc
     3181 tgtgtgtgcc cacattggtc tcaaaccaca gtccattcac aaattgggtg ggtataaggt
     3241 acagggcagg tcaaaactcc aggcagaaca gattgtcgcc gatgccaaaa ttctggaaga
     3301 agcgggggcg ttttcgcttt tattggaagg tatcccgctg gaggtggcgc gtgagatcac
     3361 acagacggtt gccatcccta ctattgggat cggcagtggc ccgtattgta gcggacaggt
     3421 gctggtgtgt tacgacgtgt tgggtgccga tcccgacttt gagcctcgtt ttgtcaaaaa
     3481 atacctgaat ctgcacgaac aggtgaccca ggccttttcg gcctttgcgg ccgaggtcaa
     3541 agctggtcag ttcccatcgg acaaacattg ttttcatcag aatgtggtgg cggtgaaagc
     3601 gatcaagtga atggggaacg gataatgggt aatgggttaa tgattttctt ggctatgaat
     3661 tagatttttc aggtcttcaa tttccttttc caattcaata ttttttctca ggaggtcgtt
     3721 gtatttgttt tgcagctgtg ccttttcttc ggcaagtctg gagacatcgt tgccgactgc
     3781 cggagaaggc tcaaaataag ctgctccgga atctggcagt gttcccgcta cgggttgtgg
     3841 ttcgctgaca gggactgatt gtatttcgac tgctgtgttg gggggaacga cggtctccaa
     3901 ggcagggagt gcagagggag agacaggctg gttgttttcg ggggctgtct cagcggggat
     3961 ttcattggca ctgttgggtg tttctgtctc tcggatttga tgtggctctg ctactgggtc
     4021 aacagccggt tcctctttgg gaagagattt ctttggcctg gttttgtgtc tatcttgatc
     4081 gtgttgactg aggtatccct gttgggcaaa tcgttttgtg ataaacgatg aatcaaaacc
     4141 cggcatgaag tggccgtaca acacgaggcc gatggtgagg gtgtatgcga aaacaagcaa
     4201 ccaggatgcc aggttgatga gcccatgaaa tctcttgagg ggactcagca gatacggg
//
LOCUS       DATDUN010000463         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_356194, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000463 DATDUN010000000
VERSION     DATDUN010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356194"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VJC18_06470"
     CDS             complement(<1..753)
                     /locus_tag="VJC18_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45063.1"
                     /translation="MWTFRTASPFSNVFSLTNLYSPTALAKIPASVPPLELIPPSSHL
                     VLDGYRLQSDPRTGSSIVDTSHLRTNSVRQEIVPALAPYTRFAPQVAATSSTITRDPD
                     ETKQVTNLGLGILSTLQIADPQSFGDIKASELSATIDALRTANITQLISDSPFVQMTE
                     NAVGPPASLAHYLKLDQLQTVDYGQMAYVLQLGVKVGAFAALNVASWYGSGFLQKIIQ
                     NVINSSELSEGKKHFLTQSAKATRITFVAMAAF"
     gene            complement(1052..1603)
                     /locus_tag="VJC18_06475"
     CDS             complement(1052..1603)
                     /locus_tag="VJC18_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45064.1"
                     /translation="MKKQHFIFALLLVALIAVATNANAFSMKGEKVFGVQALGYYELN
                     SDWESLYFDFAYNGSTQTAGFGFGSFFELGLSDRMSMEIDLAYSRLTYSNRQINIITE
                     NYFTADVVGHYYFLDNETYNPYFIFGLGAIMSANSVAPLGDVGVGMHFRVSKEFSVKT
                     ELLFKSAVLLNRGEGRLGVCYHF"
     gene            1813..>2175
                     /gene="eno"
                     /locus_tag="VJC18_06480"
     CDS             1813..>2175
                     /gene="eno"
                     /locus_tag="VJC18_06480"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985492.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLD45065.1"
                     /translation="MSKITYINAREVLDSRGNPTVEVELVLDNAVSARALVPSGASTG
                     EHEACELRDNDPHRYLGKGVLKAVQNVNTEITKKFIGFPANEQVRLDKELCALDGTEN
                     KSRLGANAILGVSMAYARA"
BASE COUNT          622 a          469 c          586 g          498 t
ORIGIN      
        1 aaaggcggcc atagcgacaa aggtgatgcg ggtggccttt gcagactggg tgagaaaatg
       61 tttttttccc tccgagagct ccgagctgtt gatgacgttt tgaatgatct tttgcaaaaa
      121 cccggaacca taccacgagg ccacgttgag ggcggcgaag gcccccactt tcacacccaa
      181 ttgcaaaacg taggccattt gcccgtaatc aacagtctgt agttgatcca gtttaagata
      241 gtgggccaga gaggcggggg ggccaacggc attctcagtc atttgcacaa agggactgtc
      301 gctgatcagt tgggtgatat tcgcggttcg cagggcatcg atggtcgctg acaactccga
      361 cgccttgatg tctccaaaag attgtggatc ggctatttgc agggtcgaca aaatacccaa
      421 gcccaggttc gtgacctgct tggtctcatc aggatctctc gtgatggttg atgaggtggc
      481 ggctacttgt ggcgcgaaac gggtgtatgg ggcgagcgcg gggacgatct cctgccgaac
      541 tgaatttgtc cgaaggtgac tagtgtcaac aatagatgat cctgtccgag gatcactttg
      601 caacctgtaa ccatcaagga caagatgtga ggagggagga atgagctcta aaggaggcac
      661 actggcgggt atcttggcta gcgctgtcgg tgagtacagg ttggtcaggg aaaagacatt
      721 cgagaaaggg gacgctgtac ggaaagtcca cataaacagc ctttctctaa tcccaaaaaa
      781 atgctcaaat tgtatcgcta tccagattca gtcgttttac gatctgggat catgatgaaa
      841 ttgtcggagt tatgccatga aatagcaata tgaatatcag gacaattaat atcaaaagaa
      901 tgagggaaaa gctgatgtga caagggctat cgttaactaa gaggaattac tcaacaaaga
      961 attcttgcgc gtcgatgtaa acaatcgcac tctgtcgcaa taactaaaaa cgtaacgcag
     1021 agggatgcaa aacaaggcgc tgatcgatcc gctaaaaatg ataacagaca cccaaacgtc
     1081 cttcgccgcg gtttagaaga acagcggact tgaacaggag ttctgttttt acagagaact
     1141 ctttggacac cctgaagtgc atccccacgc cgacgtcacc caatggagcc acggagttgg
     1201 cgctcatgat ggcgcccaga ccgaagatga aataggggtt gtaggtctca ttatcgagaa
     1261 aataatagtg acccaccaca tcagcggtga aataattttc cgtgatgatg ttgatttggc
     1321 gatttgaata ggtcaaccgg ctgtacgcca gatcgatttc catcgacatg cgatccgaca
     1381 aacccagttc aaaaaacgaa ccaaacccaa aacctgccgt ttgagtactg ccgttgtagg
     1441 caaagtcaaa atacaggctt tcccaatcgg aattgagttc ataataaccc agagcttgca
     1501 ccccaaagac cttttcgcct ttcatggaga aggcgttggc gtttgtggca acagcgatca
     1561 gggcaaccaa tagtaaagca aaaataaagt gttgtttttt cataggggga gactctaatg
     1621 caggtgatac gatttggtca ataggaacgt gtgaggttta aggcttaaag ctcagggctc
     1681 aaggcttacg gcaggaggac tatggcgtgg agaatggata aaacacttgt gtcgaggcct
     1741 tttgaggtag tataagcgcc cacgaaaata cacggcagtc aaaaacacag tttttttgga
     1801 aaggaaaaat ttatgtcaaa gatcacatat atcaatgcca gagaggtttt ggattcacgc
     1861 ggtaatccca cagtcgaagt ggaactggtt ctggataatg ctgtttcggc cagggccctg
     1921 gtgccttcgg gcgcgtcgac gggggagcat gaggcctgtg aattgcgcga taacgatccc
     1981 caccgctatc tgggtaaagg tgtgctgaag gcagttcaga atgtaaacac agagatcaca
     2041 aaaaaattca ttggttttcc agcaaacgaa caggtcaggc tcgataagga actctgtgcc
     2101 ctggatggca cagaaaataa atcacgcttg ggagcgaacg cgatcctggg agtgtcgatg
     2161 gcctatgctc gggcg
//
LOCUS       DATDUN010000464         1108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_312336, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000464 DATDUN010000000
VERSION     DATDUN010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312336"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VJC18_06485"
     CDS             complement(<1..216)
                     /locus_tag="VJC18_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45066.1"
                     /translation="MSEKQGLTLAFVISLVGGLIVLVFSLVHLLWFGSNAPNWGGFGN
                     YWGGMMDGYHNFMGSYSSSTGFFTMISA"
     gene            complement(226..>1108)
                     /locus_tag="VJC18_06490"
     CDS             complement(226..>1108)
                     /locus_tag="VJC18_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLD45067.1"
                     /translation="VDENESLYVGCHGGVGSTAAGELMTKADLLIVIGSSFSDLSLIP
                     QKRTIQIDINPLMIARRYPVEVGLLGNSAVLIPKLTDKVAQKQNMDYLAEVARLKQAW
                     VEQLEKETSSTLKPIRPQYIIKVLNQKIADNAVISLDVGENCWWFGRNFQMKKTQKMV
                     MSGGLATMGFGLPGALAAALAYPERQIVCLTGDGGLTMVLGDFLTALKYNLPVKVFVM
                     NNKRLGMIMQEQKVEGYESWQTDLFDYSFADFAEHSGGLGIKVTEPNELDAAVDKALK
                     SDKPTIVDIDTDPRRFL"
BASE COUNT          291 a          290 c          219 g          308 t
ORIGIN      
        1 cgccgagatc attgtgaaga aacccgtcga gctgctgtag ctgcccatga agttgtggta
       61 gccatccatc ataccgcccc aatagtttcc aaagcctccc caatttggcg cgttggagcc
      121 aaaccacaat aaatgtacca aactaaagac aagaacaatt aagcctccga ccagcgaaat
      181 cacaaacgcc aacgttaacc cttgtttttc actcaaactt ccacctcaca gaaaccttct
      241 tgggtcagtg tcaatgtcca cgattgtggg tttgtctgat ttgagggctt tatccacggc
      301 agcatccagc tcatttggct cagtgacttt gatgcccaat cctcctgagt gttcggcaaa
      361 gtcagcgaag ctgtaatcga ataggtccgt ttgccagctt tcatagccct ccactttctg
      421 ctcctgcata atcatgccaa gtcgcttgtt gttcatgaca aaaactttga ctggcaaatt
      481 atatttgagt gcagttaaga aatcgcccag aaccatggtt aagccgccat caccggttaa
      541 acacacaatc tgcctttcgg gatacgccaa agccgcagcc aatgctcctg gcagaccaaa
      601 acccatcgtc gccaatcctc cactcatgac cattttctga gttttcttca tttggaagtt
      661 tctgccaaac caccaacagt tttcgccaac gtctagcgaa atgactgcgt tatcggcgat
      721 tttttgattt aagaccttga ttatgtattg tggcctaatt ggtttcaagg tagaacttgt
      781 ttctttttcg agttgttcta cccaagcttg ttttagccga gcaacctcag ctaaataatc
      841 catgttttgc ttttgtgcaa ccttatctgt cagcttgggg atcagaactg cgctgttgcc
      901 caacaagcca acttcaactg ggtatcggcg agcaatcatc aatgggttaa tgtcaatttg
      961 tattgtgcgt ttctggggaa taagcgacaa atccgaaaac gaggagccaa tcacaatcag
     1021 caaatctgcc tttgtcatta attcgcctgc agctgttgag cctacaccgc catgacaacc
     1081 aacatagagt gactcgtttt catcaact
//
LOCUS       DATDUN010000465         6541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_356222, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000465 DATDUN010000000
VERSION     DATDUN010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356222"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VJC18_06495"
     CDS             complement(<1..1628)
                     /locus_tag="VJC18_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45068.1"
                     /translation="MTQVQFAPNANPLTDAEFNQFEITPDGVIYLDELATKQLVDEFI
                     SRFVKTSVPSTIDLYVGGMSLSLVLKTREALLALQDNPELMSGLTDEDKKRIKNWLEN
                     EFTINGVYANFSAGYEYSTNEESGHYELVDGQNSIYEMLSAQGETSHTPWVYGSASGE
                     HTGPLWKHNWSMGQMFAGSWSNQYQDVEYIVDDVALPQDPYREKDDTKIFKTKAGVSF
                     ERTDDKGDFSAGGYYKYYLEPMADSKQDELSAQLNGQINKIRGSGLSCEGGASFFQNQ
                     YTEPNVVDQQARSGRTFTANLEPSLQLVDRFGLVMEAQAERGDSTSYYSTDKRTDVSV
                     SLLGQIALNKKEKAEAETAQDKPQANLRVGAGGSYQANDTERTDRPEEVITQEGDAYA
                     EGSFSLAGPKIGLKIYTDGRLNFSYGSLVGIYPSSYQRISLTYTPKKGSLGFGVSHFG
                     YRWTLDYIHLPGAEAYFEEGEYSKTSHAISASVDGKVAPKDFLELNASGSVGRTLQQG
                     AWAYEYMSYAAEAGVSLRLFPSNPEVWLSVSGDYS"
     gene            1832..3289
                     /locus_tag="VJC18_06500"
     CDS             1832..3289
                     /locus_tag="VJC18_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45069.1"
                     /translation="MKPITIKTHNNFVGRERECQVLQKIAQQNEASIVVVYGRRRVGK
                     TALIEQTLRHRNLLKFEGIEGQPAVRQKENALYQLSKYAKDPLFAKVQIKSWLEFFDI
                     LAPFVAQGQWTLYFEEVQWLANYENDFITELKHAWDNCFRHNSQLAIVLCGSSPSFMI
                     RHVLESRALYNRSQHEIQLKEFDLLEARNFLHKRSLREVMDAYLSVGGIPEYLKWVNR
                     ESSVMLGLCHNAFVSDGFFVNEHKRIFTSSLAQNKNYKKIIQYLSKKRFATRNEILKH
                     LKTESGGTLSALLLDLEMAGFITRYVPFYLVEKSALARYHISDFYLQFYFKFIQPIEK
                     NIAAGDYNKNPISALPQTSYQTWAGFAFERVCKKFHRVIARILGFDSVKYRVGAFFSR
                     ETQKQDAGCQIDLVFDRDDRVYTICEIKYTQGLVGLSVLKEFENKLAIFPNRKKHTIQ
                     KVLITTDGADTALTNRVYFDRIITLKDIFNPHYWG"
     gene            complement(3315..4931)
                     /locus_tag="VJC18_06505"
     CDS             complement(3315..4931)
                     /locus_tag="VJC18_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45070.1"
                     /translation="MKRYIVIICIFCVSVLLSATVLASAQIKRRAYNGIEPVVAEVSP
                     AATQQQNIVVTTDVPNTLPESYYNSLKPLPSDTTAPSADPETEVVEEATSPPQPTINV
                     VKTQDTPVVQRVQPEAVAPVAEPASSSIPEPVTEEKKPKQRREHSFQSNQYNTSYILT
                     EIGNLRQELAEMRTGKGEGKSSGYDGGFFIQSEDGKFKIIFSGRLQFKYSFILAEDLE
                     DGHTFNLRRLYFNIDGNAFSEKFTYNITLSPASSTSLYTFAIGYAFHPAFIVNATKQP
                     GGISNIVLSSGGLMFISVPLTAFRFDYGDAVGLETTGEIGWFSYKLSVANGQALAYKE
                     NTNTEMFYTSRFDFNVLGKMDSAQGDIAYSESPIFTFGFGVAFGHMDDGTQARVLGGA
                     GDTRFKYKGFSLIVGGNYRQVDPDQFTRAQTDVGATVMASYFVIPKKLEIALRGDALL
                     DDITDAGVNLMMDGANDDGFFAPNVATLSGGDIDGDSANEYAGTLCMGYYFAGKNAKI
                     QAEYTFNLDGIVGPDDRVYHVGQVQVQLGF"
     gene            complement(5063..5872)
                     /locus_tag="VJC18_06510"
     CDS             complement(5063..5872)
                     /locus_tag="VJC18_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45071.1"
                     /translation="MGVTMSICSKKVFILCLVVLFSFLFVNCDSNSVNNDDVNPNVSA
                     VTDDVATNVGPVDLLVSTDVLAKVNTIRGKLEDYIAEVTGSYDEPGAYNQVKFSINPG
                     GDEQSVTIDLSEDIDCTTAGTKNIIGSATFTLGASQNSGTLVGSFVIQYSSCQDIIVL
                     ETESGTCAITPTVTGELQTDINLEYVLGGNDVSGQGISDTTEVSSGTSVALDVSVGST
                     AADQSYSFTYYLSTSLSSNGLDGIVSYSGNSYDMGDVESYIENSATTAVCE"
     gene            complement(5959..>6541)
                     /locus_tag="VJC18_06515"
     CDS             complement(5959..>6541)
                     /locus_tag="VJC18_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLD45072.1"
                     /translation="RADLFDKAVEILRLVGLDEDVMLKYPHEFSGGQRQRIGIARALS
                     VNPKLIIADEAVSALDISIQAQIINLVQDIKEQFGLTLIFIAHDLKVVEHVSDTICVM
                     YLGRVMELFRAVNMNTVQHPYTKSLISAIPVPDPTVKKQRQILEGDVPSPINPPTGCV
                     FHPRCPRALPECAQKVPQLRRIGENHQIACDLV"
BASE COUNT         1764 a         1631 c         1396 g         1750 t
ORIGIN      
        1 gaatagtcac cgctcaccga taaccagacc tcggggtttg agggaaataa tctgagactc
       61 acgcccgctt cagcggcata actcatatat tcataggccc aagccccctg ctgtagcgtg
      121 cgccccacag aaccagacgc attcaattcc agaaaatctt tgggtgctac cttgccatcc
      181 acacttgcgc tgatcgcatg ggatgttttt gaatactccc cttcctcaaa ataggcttcg
      241 gcccccggca agtgaatgta atcaagcgtc caccggtatc caaaatgact caccccaaat
      301 cccaacgacc ctttttttgg ggtataagtc aaagagattc tttgatagga cgaaggataa
      361 atgccaacca atgaaccgta tgagaaattc agcctgccat ctgtataaat tttcaatcct
      421 atcttaggac ccgcgaggga aaaagaccct tcggcatagg catcaccctc ttgggtgatg
      481 acctcctcgg ggcgatctgt tctttcggta tcgttagcct gatacgatcc tcccgcgccc
      541 actcgcagat ttgcctgtgg tttgtcttgc gccgtttctg cttctgcttt ttctttttta
      601 ttcagcgcga tctgacccaa aagcgaaaca gacacatctg tccgcttgtc tgtagaataa
      661 taactggtgc tatcacccct ctccgcctgc gcctccatca cgagtccaaa acgatcgact
      721 agctgaagtg acggttccag attggccgta aacgtcctgc cagagcgtgc ctgctggtca
      781 actacgttgg gttcggtgta ttgattctga aaaaaactgg cacccccttc gcatgagagg
      841 ccagaacccc tgattttatt gatctgaccg ttcaattgag cgctgagctc gtcttgtttg
      901 ctgtctgcca ttggttccag atagtattta taataccccc cggcagaaaa atcacctttg
      961 tcatcagtgc gttcaaagct cacccccgcc ttggttttaa agattttggt gtcatccttt
     1021 tcgcgataag gatcctgggg cagggccaca tcatccacaa tgtactcaac atcctgatac
     1081 tgattagacc aggagcccgc aaacatctgc cccattgacc aattatgttt ccacaacggg
     1141 cccgtgtgtt ctcccgaagc ggagccatag acccacgggg tgtggctggt ttccccttgg
     1201 gcagacagca tttcgtaaat gctattttga ccatccacca gttcgtagtg accgctttcc
     1261 tcgttcgtgc tgtattcgta acccgcggag aaattcgcat aaaccccgtt gatggtaaat
     1321 tcgttttcca gccaattttt gattcttttt ttgtcttcgt cggtcagtcc ggacatgagt
     1381 tcgggattgt cctgcaaagc caaaagggcc tcccgcgttt tgagcaccag actcaggctc
     1441 atgcccccca cgtacaaatc gatggtggag ggcacgcttg ttttgacaaa ccgactgatg
     1501 aattcgtcaa caagttgttt tgttgcaagt tcatccagat aaatcacacc atcgggtgtg
     1561 atttcgaact gattgaattc agcatcggtg aggggattgg cgtttggtgc aaattgaact
     1621 tgggtcatgg gtgtccattt gtattgtgtg cgcttgtact gataatatgg atgctgtgaa
     1681 aaaggcaaca cacattgcac gaataaaagc ctgtccgtat agccaacttg ccataataat
     1741 agtcgtagtt ggccataata tgcatatcga ttctagccaa ctaacactat tttattgcct
     1801 tgttggcttg aatacattat tctcaggttg tatgaaaccc atcaccatta agacccacaa
     1861 taattttgtc ggcagggagc gcgagtgcca ggtccttcaa aaaatcgctc aacagaacga
     1921 agcgtccatc gtcgtcgtct atggtcggcg tagagtcggg aagaccgcgc ttatagagca
     1981 aacactgcgc catcgtaacc tcctcaagtt tgagggtatt gaaggtcaac cggcggtcag
     2041 acaaaaagaa aacgctcttt atcaactgtc gaaatacgca aaagatccct tgtttgcaaa
     2101 ggttcagatt aagagctggt tggagttttt tgacattctg gctccttttg tggcccaagg
     2161 gcaatggacg ctgtactttg aagaggttca atggttggct aactatgaga acgattttat
     2221 cacggagctt aaacacgcct gggacaactg cttcaggcac aattcccaat tggccattgt
     2281 gttatgtggc tcttctccgt cattcatgat tcgacatgtt cttgaatccc gtgccctcta
     2341 caaccgatcg caacacgaaa tccagctgaa ggaatttgat ctgctagagg ccagaaattt
     2401 tctgcacaaa agatcgttac gcgaagtcat ggacgcttat ctgagcgtgg ggggcattcc
     2461 cgaatatctc aaatgggtga acagggaatc ttctgttatg ctggggttat gccataatgc
     2521 ttttgtttca gacggcttct ttgtaaatga acacaaacga atattcacca gcagtcttgc
     2581 ccagaataaa aattacaaaa aaatcattca atatttaagc aaaaaacgtt ttgcgacacg
     2641 caacgaaatc cttaaacatc tcaaaaccga atcagggggg actctgagcg ccctcctgct
     2701 ggacctggaa atggctggtt tcattacccg ctatgtccct ttttatctgg ttgaaaaaag
     2761 cgcactcgcc agataccaca tcagtgactt ttatcttcag ttttatttca aattcataca
     2821 gcccatcgaa aaaaacatag ccgccgggga ttataacaaa aatcccatta gcgccttacc
     2881 ccagacatct tatcaaacgt gggctggctt tgcttttgag agagtgtgta aaaaattcca
     2941 tcgggtcatt gctcgcattc ttgggtttga ttctgtcaag tatcgcgtcg gtgctttttt
     3001 cagccgcgaa actcaaaaac aagatgcggg ttgccaaatt gatctggttt ttgatcgaga
     3061 tgatcgcgtg tacacgatct gcgaaatcaa atacacacag gggttggttg gcctatcggt
     3121 gctcaaagag ttcgagaaca agctcgccat tttccccaac cgcaaaaaac acaccatcca
     3181 aaaagtcctg atcacaactg atggcgcgga cacagcgctc accaatcggg tttattttga
     3241 caggatcatc acattaaaag atatattcaa tcctcattat tgggggtaat gagtcatggg
     3301 tgcaacatca aagatcaaaa gcccaactgt acttgaactt gtcccacatg ataaacgcgg
     3361 tcatcgggac caacaatgcc atctaaatta aaggtatatt cagcttgaat ttttgcgttt
     3421 tttccagcaa aataataccc catgcagagt gtgcccgcat attcatttgc ggaatcacca
     3481 tcgatatcac cacccgataa cgtagctaca ttcggcgcaa aaaaaccatc gtcgttcgca
     3541 ccatccatca tcaagtttac acctgcatct gtgatatcat ccaacaaagc atcgccccta
     3601 agtgcaattt caagtttttt aggaatcacg aaatagctag ccataacagt tgccccaaca
     3661 tccgtttgag ctcttgtaaa ttggtcagga tctacctgcc gataattccc gccgacaatc
     3721 aatgaaaaac ctttgtattt aaaacgggtg tctcctgcgc caccgagcac ccttgcctgt
     3781 gtcccatcat ccatgtgacc aaaagcaact ccaaacccga atgtaaatat ggggctttct
     3841 gaataggcaa tatctccttg ggccgaatcc atttttccca gcacattaaa atcgaacctg
     3901 ctagtataaa acatctcggt gttggtattt tctttgtagg ccaaagcctg gccgttggcc
     3961 acggacaatt tataactaaa ccaaccaatt tctccagtgg tttccaaacc aacagcatcg
     4021 ccgtagtcga atctgaacgc tgttaaaggc accgaaataa acatgagccc accagaactc
     4081 aagacaatat tagaaatccc gcccggttgt tttgtagcat tcacaataaa cgcggggtga
     4141 aatgcatagc caatagcaaa agtgtaaagc gaggtagaac tagccggaga aagtgtaatg
     4201 ttgtaggtaa atttttcact aaacgcattt ccatcgatat tgaaataaag tcttctcaag
     4261 ttaaatgtgt gcccatcctc caggtcctcc gcaagaataa aagaatattt aaactgcaat
     4321 ctgcccgaaa aaataatttt aaatttacca tcttcgcttt gtataaaaaa acctccgtcg
     4381 tatcccgatg acttgccttc tcccttgcca gttctcattt cagcaagttc ttgtcttaga
     4441 ttgcctattt ctgttaaaat gtatgatgtg ttatactggt tggactggaa agagtgttct
     4501 cttcgttgtt tcggtttttt ttcttctgtc acaggttctg ggattgaaga tgacgctggt
     4561 tccgccaccg gtgcaacagc ttcaggctga acccgttgca ccacaggtgt atcctgcgtt
     4621 ttcaccacat tgatagtggg ttgtggtggg gatgtggctt cttcgaccac ctcggtctcg
     4681 gggtccgctg aaggcgctgt tgtatcggaa ggcaggggtt ttaaggaatt gtaatacgat
     4741 tcgggcagag tattggggac atccgttgtg actacaatgt tttgttgctg ggtggcggct
     4801 ggggaaacct ctgccacaac aggctcaatc ccgttatagg cccgccgttt gatctgggca
     4861 ctggccagaa cagtggccga caacaacacg ctcacacaaa aaatacaaat gatcacgata
     4921 taacgtttca taagactctt cccttttgct accaaaccca acttcatatt aatcatccag
     4981 ctttgacttg tcattccagc gaaagctggg atccatgaat agaaaaatat ggattccagc
     5041 tttcgctgga atgacaaaca tgtcactcac aaacagcggt ggtcgcggaa ttctcgatat
     5101 agctttcgac atcccccata tcataagagt taccagagta gctcacaatc ccatccaatc
     5161 cattgctgga taaactcgtg ctcagataat atgtaaaact gtagctctga tccgccgccg
     5221 tgcttcccac agaaacatcg agcgcaacac tcgtgcctga agaaacttct gttgtatcgg
     5281 aaataccttg gccacttaca tcattacctc ccaacacata ttctaaatta atatccgttt
     5341 ggagttcacc tgttaccgtt ggtgtaatag cacatgttcc gctttctgtt tcgagtacaa
     5401 taatgtcctg acaactacta tactgaataa caaacgaacc gactagcgta ccagaattct
     5461 gtgatgcccc caacgtgaac gttgcggacc caattatgtt cttcgtacct gctgtagtac
     5521 aatcaatatc ttcactcaga tctattgtaa cactttgctc atctcctccg gggttgatgc
     5581 taaattttac ctggttatag gcacccggct catcataact tcctgtaacc tcggcaatat
     5641 aatcttcaag cttcccccta atagtattaa ccttagccag cacatcggta ctcaccaaca
     5701 agtccaccgg tcccacgttg gtggccacat cgtctgtgac cgcagacaca ttggggttga
     5761 catcgtcatt attaacggaa ttgctgtcgc agttaacaaa aagaaacgaa aacaacacca
     5821 caagacacaa aataaaaact ttttttgaac aaatggacat ggtgactccc acaaatatcg
     5881 ccaacgacct gaacctgagc attagcgata atcccaacga gaatcaagag ataatacaag
     5941 aggatggctt agaaaaaatc acaccagatc gcaggcaatt tggtgatttt ccccaatgcg
     6001 cctgagctgg ggaacttttt gggcacattc cggcaacgcc cgtgggcaac gggggtgaaa
     6061 cacacaaccc gtgggagggt tgatgggaga aggcacatca ccttccagta tttggcgctg
     6121 ttttttgacc gtggggtccg gcacggggat ggccgagatc agactcttgg tataggggtg
     6181 ctgaactgta ttcatgttta cggccctgaa cagttccatc accctgccta ggtacatcac
     6241 gcaaatcgtg tcggaaacat gctccaccac cttgaggtca tgcgcaataa aaatgagcgt
     6301 gagtccgaat tgttctttga tgtcctgcac taaattgatg atctgcgcct ggatcgaaat
     6361 gtccagcgcc gacacggcct catcggcaat aatgagtttg gggttgactg acaacgcgcg
     6421 cgcgatgcca atgcgttgac gttgaccacc agaaaactcg tgtgggtatt tgagcatgac
     6481 gtcttcgtcc agccccacca atcgaagtat ctctacggct ttatcaaaaa ggtccgcacg
     6541 c
//
LOCUS       DATDUN010000466         2824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_228653, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000466 DATDUN010000000
VERSION     DATDUN010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228653"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VJC18_06520"
     CDS             <1..378
                     /locus_tag="VJC18_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HLD45073.1"
                     /translation="KGIERMRLLGYYDHILEVDAKQAIMCQKAVAEVTDGAMADLVIN
                     CVSVENTELSSIMCAKPTSDATVYFFSMATSFTKAALGAEGIGSDALLIVGNGYAPNH
                     AKIALDVIRRHDELRSLFEKVYV"
     gene            complement(407..745)
                     /locus_tag="VJC18_06525"
     CDS             complement(407..745)
                     /locus_tag="VJC18_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HLD45074.1"
                     /translation="MKKIEAIIKPFKLDDVKEAISELGVKGMTVSEVKGFGRQRGHTE
                     LYRGAEYIVDFLPKIKIELVIKDEDTAKVVEAIQNAAKTGRIGDGKIFILPVEEVIRI
                     RTNETGEAAI"
     gene            complement(833..2047)
                     /locus_tag="VJC18_06530"
     CDS             complement(833..2047)
                     /locus_tag="VJC18_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45075.1"
                     /translation="MSKKKKKEEEPKSSLKFFLLASIVLFVFLAISIYHNSKNPFTQV
                     LQEYVVNKQEIHDFLGLVDKVSGMPTTQVVDRNTKQNVSVKLIGKTGSGTLDAVMEHS
                     LNGWRLSKATLRIGNTLHRLSPMYVRDISYNEASATADTANKPVFEAGNDIIIKTLLK
                     GIPTNNNSLRITQNLSVFDVHRKLLKQKNNVASFESSPKQKAGSSSQNEQTAITAQNV
                     TNDELTFTTKISNLSPGTYYLNMSFQDNDSALTENHWRKIIINDPKDKVAVLSILFFK
                     DQNLTEPKTDAVFTTSEDIFIRLNLAGFMVSGQSIAGTVDLKIENSHGGLVAYKPGFV
                     TFKQPYDQKKQVVVDGHLRLTEPDVYFLSFRIQDEHAHTQVIRNAKIVVRFPDFEKPA
                     TPEPVASPPTKD"
     gene            complement(2112..>2824)
                     /locus_tag="VJC18_06535"
     CDS             complement(2112..>2824)
                     /locus_tag="VJC18_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45076.1"
                     /translation="AGSAQDKKVCIKWDITFEDPVISSRLTSPGILYHRWFANTKFLQ
                     PRALGYGSGTLYVDGHKKELKRARLHQGHLYGTAYTQNWIQGNCESFQEDSEAFFNAV
                     SFKRLYLKKFSRTLTLCAIGMEGKKFVATSPVQALFCNKTSYADNTWTLQFTCRGYRF
                     ICRLKRRPELTAGVRHSGINGSQAYFYHCPLADVEIKVYKKNRGAWLFYKTLSAQGRG
                     SFETMSSEKEASLSLTES"
BASE COUNT          699 a          607 c          703 g          815 t
ORIGIN      
        1 aagggcatcg agagaatgag gctgttgggc tactatgatc atatcttgga ggttgacgca
       61 aaacaggcca tcatgtgtca gaaggccgtt gctgaggtga ccgacggtgc catggcggat
      121 ttagtgatca attgtgtgtc agtggaaaat acggaactgt cttcaatcat gtgcgccaag
      181 ccaaccagtg atgcaaccgt gtattttttt tccatggcaa cgagttttac aaaagcggcg
      241 ctgggggcag aagggattgg gtctgatgcc ctgctgattg ttggaaatgg atacgcaccc
      301 aaccacgcaa agatcgcgct ggacgtcata cggcgtcacg atgaactgcg tagcttgttc
      361 gaaaaagtct atgtgtaaat atgctgaagg atccttcgaa cttggtttaa atggcagctt
      421 cgcctgtctc attggtgcgg atgcggatga cttcctcgac aggcagaata aaaattttac
      481 cgtctccaat gcggccagtc ttggcggcgt tttggatggc ctcgaccact tttgcggtgt
      541 cctcgtcttt gatgacaagc tcaattttta tttttgggag aaaatccacg atgtattcag
      601 ccccccgata gagttcggta tgtcctcttt ggcgaccaaa accctttacc tctgaaacgg
      661 tcatgccctt cacgccgagc tcagaaatgg cttctttgac atcatccaac ttgaaaggct
      721 tgataatggc ctcaattttt ttcatatccc cccattgata aacactcatc ctatgtttgc
      781 tggcaaagcg taacaacaat agtatcagaa aacaagggca aacaacaccg ctctagtcct
      841 ttgtcggggg cgatgccacc ggctccggag tggcgggttt ttcaaaatct ggaaaccgca
      901 caacaatttt tgcattacga atcacttggg tgtgcgcgtg ctcgtcttga atcctgaaag
      961 acaaaaagta gacatctggc tctgtgagcc tgaggtgtcc gtcaactacg acctgctttt
     1021 tctgatcgta tggttgtttg aaggtcacga accctggctt gtaagcaacg agccccccgt
     1081 gtgaattttc gatttttaaa tcaaccgtgc cagcgatgct ttgtcccgaa accatgaagc
     1141 ccgccagatt tagccgaatg aaaatatctt cgctggtggt aaagacagcg tctgttttgg
     1201 gttcggtcag gttttgatct ttaaaaaaca ggattgagag gaccgcgact ttgtccttag
     1261 ggtcgttgat gataatcttg cgccagtggt tttctgtcaa ggcggagtcg ttgtcttgaa
     1321 aggacatgtt cagatagtaa gtgcccggag acaggtttga aatttttgtg gtgaacgtga
     1381 gctcgtcgtt ggtcacattt tgtgctgtga tcgctgtttg ctcgttttgt gaggaagaac
     1441 ctgctttctg ttttggtgag gactcaaaac tggcgacatt gtttttttgt ttgagcaatt
     1501 tacgatggac atcgaataca ctcaaatttt gtgtgatgcg gagactgttg ttgttggtgg
     1561 gaatgccctt gagcagggtt ttgatgatga tgtcgttgcc agcttcgaaa acgggtttgt
     1621 ttgctgtgtc ggctgtggca gaggcctcat tgtaagaaat atcgcgcacg tacatggggc
     1681 tgagcctgtg aagggtgttg ccaatacgca aggtggcctt ggaaagacgc cacccgttca
     1741 gagagtgttc catcacggca tccaaagttc ctgatcctgt ttttccgatc aattttacgg
     1801 acacgttttg tttcgtattc cggtcaacaa cttgggtggt tggcatcccg ctaaccttgt
     1861 cgactaagcc taaaaaatca tgaatttcct gcttgttcac aacgtattct tgcaaaacct
     1921 gagtgaaagg attttttgaa ttatgataga tgctgatggc cagaaataca aataagacga
     1981 tggatgccag caaaaaaaat tttaacgaag acttgggttc ttcctctttt ttcttctttt
     2041 tagacattgt gggttcctgt aaagcttttt tgttgccgta gctcaagttg tttttttacc
     2101 caaaaacaac cttaactctc cgttaaagac agcgaggcct ctttttcgga agacattgtt
     2161 tcgaaagagc ctcttccctg tgccgaaaga gttttgtaaa aaagccaggc tccgcggttt
     2221 tttttgtaga ccttaatctc tacatcagcc aaggggcaat gatagaagta agcttgtgaa
     2281 ccattgatac cagaatgtct tacccccgct gtcagctcag gccgtctctt gagacggcag
     2341 ataaaccgat aaccccgaca ggtaaattgt aaggtccagg tgttgtctgc gtaagaggtt
     2401 ttattgcaaa acaaagcctg aactggcgag gtggcaacga attttttacc ttccataccg
     2461 atggcacaca gggtcagggt gcgcgagaat ttttttaaat aaagccgttt gaagcttacg
     2521 gcattgaaaa aagcctctga atcttcctga aaagactcac aatttccctg aatccagttt
     2581 tgggtgtaag cggtgccgta gaggtgccct tgatgcagac gagccctttt aagctctttc
     2641 ttgtgtccat caacatagag ggttcccgaa ccgtaaccca gagcgcgtgg ttggagaaat
     2701 ttcgtgttag caaaccagcg atgatataaa atacccggag aggtgagacg ggagcttatg
     2761 acagggtctt caaatgtgat atcccatttg atgcatactt ttttatcttg ggcggaccct
     2821 gcca
//
LOCUS       DATDUN010000467         1174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_147540, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000467 DATDUN010000000
VERSION     DATDUN010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147540"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..894
                     /locus_tag="VJC18_06540"
     CDS             175..894
                     /locus_tag="VJC18_06540"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HLD45077.1"
                     /translation="MKKTAFVLGILGMLLVSSYVVASQQPVPSSPKAKTIVESVTPEL
                     TRELDRKGLELGSPVFIRIFKESKELEVWLQDDMAYRHFKTYKICRFSGSYGPKQQAG
                     DHQSPEGFYKVTPGQLNPNSNYHLAFNLGYPNDYDRQFGRTGGSLMIHGNCGSRGCYA
                     MTDERIEEIYALTEAALRNGQRYVPVHAFPFKLTDYKLARHKDSQWLSFWKNLKQGYD
                     YFEQFKRPPIVFAGEREYVIY"
     gene            complement(904..>1174)
                     /locus_tag="VJC18_06545"
     CDS             complement(904..>1174)
                     /locus_tag="VJC18_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLD45078.1"
                     /translation="LPTPIAALPPQNQPADHDPASPLAIPETAKELRKAKKMLKDKFY
                     GELEKNFVLQVLKKSNGNITHAARLAGMQRPNFHALMRKHNITNK"
BASE COUNT          288 a          243 c          316 g          327 t
ORIGIN      
        1 aaaaatcaag gtcaatcaga ttaccgagca tttataacgt ttagatccta tcacaagaga
       61 tggtccatcg tggggaggcc tcttggggga tatggtttta actcattggt attgtttgtg
      121 taatttttgt gattttcgag caacatttac tctgttgcga ccgataatac ccttatgaag
      181 aagacagctt ttgtattggg catcctgggc atgctattgg tatcatctta cgtggtggcg
      241 agtcagcaac ctgtgccatc gagtcccaag gccaagacga ttgtggaaag cgtgacaccg
      301 gaactcacaa gagagttgga tcgcaaggga ttggaactgg gttcacccgt tttcatacgc
      361 atctttaaag aatcaaaaga actcgaagtg tggttgcaag acgatatggc gtatcgccat
      421 ttcaagacgt acaagatttg ccgtttttct ggaagctatg ggcccaaaca gcaagcggga
      481 gatcaccaaa gtcccgaggg gttttacaag gtcacacccg ggcaactgaa tccgaacagc
      541 aattatcatc tggcttttaa cttgggatat cccaatgact acgacagaca atttggcagg
      601 actggcgggt cgttgatgat ccatggcaat tgcggatcac gtggttgtta cgcgatgaca
      661 gacgaacgca tcgaagaaat ctatgccttg acggaagccg ccttgaggaa cgggcagcgg
      721 tatgtgccgg tgcacgcttt tccctttaaa ttgaccgatt acaaactggc acggcacaaa
      781 gattcgcagt ggttgagttt ttggaaaaat ttaaaacagg ggtacgatta ttttgagcag
      841 ttcaagcgcc ctcccatcgt gtttgccgga gagcgcgaat acgtgatcta ttagcggtgc
      901 ccatcattta tttgtgatgt tgtgctttct catcaacgca tgaaaatttg gcctttgcat
      961 ccccgccagg cgtgcggcat gggtgatgtt cccgttggac ttcttgagta cttgcaacac
     1021 aaagtttttt tcaagttccc catagaattt atctttgagc attttcttgg cctttctgag
     1081 ttctttggct gtttctggga tggccaaagg agaagctggg tcgtggtcag ctggttggtt
     1141 ttgtggcggc agggcggcaa tgggggtggg gagg
//
LOCUS       DATDUN010000468         2385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_63787, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000468 DATDUN010000000
VERSION     DATDUN010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63787"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VJC18_06550"
     CDS             <1..1283
                     /locus_tag="VJC18_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HLD45079.1"
                     /translation="AATMVTAGVYLIARLNWLFVMSPAALIVIASIGAFTAFVGATIG
                     FAQNDIKKVLAYSTVSQLGYMMLAMGAGAFSAGMFHLTTHAMFKACLFLGSGSVIMAM
                     HHEQDMRHMGGLKKHMPITFLTFVIATVAICGIFPLAGFFSKDEILWQVFHKGSFAGW
                     YYFLWAMGFVGAAGTAFYMVRLVVMTFLGESRAVPSGHGAQQHVVPKESPWNVTLPLV
                     VLASMSVVLGFLNIPYIMNHLFGSFSAGLFTEWLSPVIPVAEHGAGLEENGSHGSGLL
                     EWGVMFLSVAAAAGAMYLSYVMYTRRMDVVQRFTTKFARLHNVVLNKYYVDEAYQRFV
                     VDPLLGLNRLCAKFDAVVIDGIVNLVAKVTKLYSVVIGWCDDFFVDGLVNGLARLTNR
                     MGARLRLIQTGHIQHYAYVVVAGIVVMLMVKILF"
     gene            1293..>2385
                     /locus_tag="VJC18_06555"
     CDS             1293..>2385
                     /locus_tag="VJC18_06555"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLD45080.1"
                     /translation="MQHSTILTWITFIPLLGVVLIPFLPKMLEGFAKWASLIIATVQL
                     GMAIKIYQFFDGEAALFQFTHGPLSWIPTFHINYYLGIDSISVSMVLLTALICFVAVI
                     ASWGISKHVKAYYALLLLLDMGMMGTFVALDFFLFYLFWELMLLPMYFLIGIWGGPRR
                     EYAAIKFFLYTLAGSLLMLLVMIALYLYSDGNLFDLTQLADVSRHTGLLAHRDGFSMF
                     RFLCWLGLFISFAIKVPMFPFHTWLPDAHVEAPTAISVILAGVLLKMGTYGMLRFNYM
                     IFPDITAYCAQVVAVFGVINIIYGALCAMAQTDLKKLIAYSSVSHMGFVLLGMSAFTS
                     EGITGAVMQMFNHGTITAMLFIVVGIIYDR"
BASE COUNT          430 a          551 c          691 g          713 t
ORIGIN      
        1 acgcggcaac gatggtgacc gccggagtgt acctgatcgc cagactcaac tggctttttg
       61 tcatgagtcc cgccgcactc atcgtgattg cctccattgg cgcgttcacg gcctttgtgg
      121 gtgccaccat cggttttgcg caaaacgaca tcaaaaaagt tttggcctat tccacggtct
      181 cacaactggg gtacatgatg ctggccatgg gggcgggcgc cttttcggct ggcatgtttc
      241 atttgaccac gcatgccatg ttcaaggcct gtttattttt aggctcaggt tcggtgatca
      301 tggccatgca tcatgaacag gatatgcggc atatgggggg cttaaagaag cacatgccca
      361 tcacctttct gacgtttgtg atcgcgacgg tggccatctg tggcattttc ccgcttgccg
      421 gttttttcag taaggacgaa attttgtggc aggtgttcca taagggatcc tttgcgggct
      481 ggtattattt tttgtgggca atgggttttg tgggggccgc cggaaccgcg ttttacatgg
      541 tgcgtctggt ggtgatgacg tttttgggtg agagcagggc tgtgccgagt ggacacgggg
      601 cacagcagca tgtcgtgccc aaggaatcgc cctggaacgt gaccctgcca ctggtcgttt
      661 tggcctcgat gtcggtcgtg cttggttttt tgaacatccc ctacatcatg aatcatctct
      721 ttggcagctt ttctgcggga cttttcaccg agtggctctc ccctgttatc cctgtggcag
      781 aacatggcgc agggcttgag gaaaacggtt ctcatgggtc gggtcttttg gagtgggggg
      841 tcatgttttt gtccgtggcg gcggcggccg gggccatgta tttgtcctac gtgatgtaca
      901 caagacgcat ggatgtggtg caaaggttca ccacaaaatt tgcccgactc cacaatgtgg
      961 tgctgaataa atattacgtg gatgaggcct atcagcgctt tgtggtggat cctctgctgg
     1021 gcttgaatcg cctctgtgcc aaattcgatg ccgtggtgat tgacgggatt gtgaatctgg
     1081 tagcaaaggt gaccaaactt tattcggtgg tcattggctg gtgtgatgat ttttttgtgg
     1141 atggtcttgt caacggtttg gccagactaa cgaatcgaat gggagcgcgg ttgcggttga
     1201 tacagacagg gcatattcag cattacgctt atgtggtggt tgcggggatc gtcgtgatgt
     1261 tgatggtgaa aattttgttt tgaggactgt gtatgcaaca ttcaacgata ttaacctgga
     1321 tcacgttcat ccctctgctg ggggttgtcc tgattccctt tttgccaaag atgctggagg
     1381 gttttgccaa atgggcctcc ctgatcatcg cgaccgtgca actggggatg gccatcaaga
     1441 tttatcagtt tttcgatgga gaggcggctc tttttcagtt cactcacgga ccgctttcgt
     1501 ggatccccac gtttcacatc aattattatc tggggatcga cagcatcagt gtgagcatgg
     1561 tgcttttgac ggccctcatc tgttttgtgg cggtcatcgc gtcgtggggg atctccaaac
     1621 acgtcaaggc atattatgcc ctcttgctct tgctggatat gggcatgatg ggcacctttg
     1681 tggcgctgga tttctttttg ttttatcttt tttgggaact catgcttttg cccatgtatt
     1741 ttctcattgg catttggggt ggaccgcgtc gtgaatacgc ggcgatcaaa ttctttttgt
     1801 acaccctggc agggtcattg ctcatgttgc tcgtgatgat cgccctgtat ttgtacagcg
     1861 atggcaattt gttcgatctg acccaactgg ccgatgtgag caggcacacg ggtctattgg
     1921 ctcatcggga tggtttcagc atgtttcggt ttttgtgctg gctggggttg ttcatcagtt
     1981 ttgcgatcaa ggtgcccatg ttcccgtttc acacctggtt gcccgatgcc cacgtggagg
     2041 ccccgaccgc gatttcggtg attctggcgg gtgttttgct caagatgggc acctacggga
     2101 tgctgaggtt taattacatg atctttcccg acatcacagc ttattgcgcg caggtggtcg
     2161 ctgtctttgg ggtcatcaac atcatttacg gtgccttgtg tgccatggca cagaccgatc
     2221 tcaaaaaact cattgcctac tcgtcggtgt cgcacatggg ctttgtgctc ctcggcatga
     2281 gcgcgttcac gtccgagggg atcacgggcg cggtcatgca gatgttcaat cacgggacga
     2341 tcacggccat gctcttcatt gtcgtgggca ttatctatga tcgtg
//
LOCUS       DATDUN010000469         1702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_228750, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000469 DATDUN010000000
VERSION     DATDUN010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228750"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..401)
                     /locus_tag="VJC18_06560"
     CDS             complement(57..401)
                     /locus_tag="VJC18_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45081.1"
                     /translation="MKGKHINHNFDLTLDPRLFGTTNQTVNDDVLKELIDHHDELRSL
                     ARQINNVEVKQMTGIMTSQTSQLPRKIERIYSKGEIRELVCRHLIPAYQHEYIIQAVT
                     NYLYTIHVSAEN"
     gene            complement(712..1134)
                     /locus_tag="VJC18_06565"
     CDS             complement(712..1134)
                     /locus_tag="VJC18_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007617735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HLD45082.1"
                     /translation="MSEQESILWQGSPSHIMNFGVYTLCALLCILVVPIFYGLYRWLK
                     LRCFRYELTTERLRTAQGILSRRVDELELYRVKDICILEPFFLRLFKKGNIVLSTSDR
                     MTPDVVIQAVPDYQELREKIRTQVETMRARKGVRELDV"
     gene            1312..>1702
                     /locus_tag="VJC18_06570"
     CDS             1312..>1702
                     /locus_tag="VJC18_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012143755.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HLD45083.1"
                     /translation="MGTLNQLQPEKLWQFFDEICEIPRISKHEQQVADYVVCFAKKRE
                     LKYLMDPVGNVVIRKKATTGYENRKPVILQAHLDMVPQKTDDSTHDFLKDPIQPIIDG
                     EWVRANHTTLGADNGIGMAAALAILDSS"
BASE COUNT          460 a          431 c          365 g          446 t
ORIGIN      
        1 agccgttgac cgtaagcctt atgccttaag ccttaagcct tatttggttg tgtgttttag
       61 ttctccgccg acacatggat ggtgtaaaga taattggtaa ccgcttgaat gatgtattcg
      121 tgttgataag cggggatcag gtggcggcaa accaactcgc gtatttcacc cttggaatag
      181 atcctttcga tctttcgggg aagctgagac gtctgggagg tcatgatgcc tgtcatttgt
      241 ttcacttcaa cattattgat ctgccgagca agtgagcgaa gctcgtcgtg atggtcaatg
      301 agctctttga gtacatcgtc attcactgtt tgattcgtcg tcccaaacag acgcggatca
      361 agggtcagat caaaattatg attaatgtgt ttacccttca tgttatccct cacactcaat
      421 acttcgccag actggaaaaa aagttgctag tatttttact ttatttttgt gccccaaagg
      481 acacagctta acctagccta gtcattggat tttgcaaggc aaaaacatat tcgggtttta
      541 caaaataagc ctggagcata agacacttgt tgtaatgggt aatgggtgat gagaaggagt
      601 ctcccataac ccataaccca ttccctctcg tgtcttcgtc cctcagcctg ccctgagcct
      661 gtcgaagggt cccctcgttt cttcgtgcgc ccccacacat ctgtggacgc ctcacacatc
      721 caactcgcgc acccctttgc gggcacgcat ggtttcgacc tgggtcctga tcttttcgcg
      781 caattcttga taatcaggaa ccgcctgtat gacaacatcg ggggtcatgc gatcggacgt
      841 gctcaacaca atattgccct tcttgaacaa tctcaaaaaa aacggttcca aaatgcagat
      901 gtccttgacc cgatacaatt ccaactcatc aacacgacgt gacaggattc cctgagcagt
      961 ccgcaacctc tcggtggtga gctcataacg aaaacacctg agtttgagcc agcgataaag
     1021 gccataaaaa atggggacga ccaaaataca gagtaaagca caaagcgtgt agaccccaaa
     1081 attcatgatg tgcgacggtg acccctgcca aagaatcgat tcctgttcag acatagacac
     1141 tccttttcgt tttctatatc gtatctggta cacaggatca aaaacaatca agtgacgtgt
     1201 ccccccggac acagaatcct ggttttcgcc gcgcaacccc ttttttatgt ttgatcttat
     1261 ttaaagaggc gtctatcggt tgacatcttt tttttaacca aggagccccc aatgggaaca
     1321 cttaatcaac ttcaaccaga aaaactatgg cagttctttg acgaaatctg cgaaatcccc
     1381 cgcatttcaa aacacgagca acaagttgct gactatgttg tgtgctttgc caaaaaacga
     1441 gaacttaaat atttgatgga tcctgtcggt aacgtggtga ttcgcaaaaa agccaccaca
     1501 ggttatgaaa atcgcaagcc tgtcatcctg caagcgcact tggatatggt cccccaaaag
     1561 acagacgaca gcacccatga ttttctcaaa gaccccattc aaccaatcat cgacggggaa
     1621 tgggtcaggg ccaatcatac cacactcggt gccgacaacg gcattggtat ggccgcagcg
     1681 cttgccattc tggactcatc tg
//
LOCUS       DATDUN010000470         1748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_189767, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000470 DATDUN010000000
VERSION     DATDUN010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189767"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VJC18_06575"
     CDS             complement(<1..205)
                     /locus_tag="VJC18_06575"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HLD45084.1"
                     /translation="MKKRSLVLLSGGLDSAYSLTVAHRDTDVVYGLCFDYGQASAKQE
                     IRASRQICVRFGIPHKVMPLPFFS"
     gene            298..1341
                     /locus_tag="VJC18_06580"
     CDS             298..1341
                     /locus_tag="VJC18_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLD45085.1"
                     /translation="MTIPKTQKAALLFGPNDVRVQEVPVPSLQQGEVLVRIMLATTCG
                     TDLKTFKRGGHPQIIKSLPSPLGHEMTGIIVRLAEGVQGFGVDDRVVIANSAPCLQCF
                     YCEKEQFNLCEDIHFINGAYSQFLAVPERFVRTNLHKIPEALAYEKAVLTEPLACVLF
                     ACQKIPVEPGETVVVIGDGPMSFLFNQVLQMQKARTIVFGLDTQRLEWIKQSGADHVF
                     DSSQVDVVKTVQGLTEGHGAAVAIEAVGLPQTWEQAVQLVCKGGRVLCYGGCAKGTEM
                     KLDTYRLHYDEISILGTFHYTPSVMDEAIRLLAEGRVNTAILTAEQRTLNDLQNIFLG
                     KDPKPALKYLIRP"
     gene            complement(1396..>1748)
                     /locus_tag="VJC18_06585"
     CDS             complement(1396..>1748)
                     /locus_tag="VJC18_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HLD45086.1"
                     /translation="RHKTGALITLAVTSPAVLKGAEPDVMDCLIRYSEAIGLCFQVVD
                     DILDIEGDESLGKPIGSDQAKNKSTYPALLGLDGAKKEAKRLLEVALASLTSFDERAD
                     PLRAIARFIAERRN"
BASE COUNT          432 a          417 c          464 g          435 t
ORIGIN      
        1 cactgaaaaa cggcagaggc atgaccttgt gagggattcc aaaccgtaca cagatttggc
       61 gtgaggcgcg gatttcttgt ttggcactgg cctgaccgta gtcgaagcac aaaccgtaaa
      121 caacgtctgt gtcccgatgc gccaccgtga gggaataggc agagtccagg ccgccggata
      181 acaatacgag ggagcgtttc ttcatccctt attgataaaa gggcggggtg agtggtgtca
      241 attgattttg aattgattgc cccatcaaac tgcggtaacc gtgaaccaaa ggttcatatg
      301 acaataccca aaacacaaaa agccgccttg ctatttggcc ccaacgatgt gcgcgtgcag
      361 gaggtgcctg ttccgtctct gcaacaaggt gaggtcttgg ttcgcatcat gttggctacg
      421 acctgcggga cggaccttaa aactttcaaa cgtggtggac atccgcagat catcaaatcg
      481 ttgccgtcac cgctcggtca tgaaatgacg gggatcattg tgcggcttgc tgagggtgta
      541 cagggttttg gcgtggatga tcgtgtggtg attgccaatt cggcgccttg cctccaatgt
      601 ttttattgtg aaaaagaaca attcaatttg tgtgaagaca ttcatttcat caatggggcc
      661 tattcccagt ttctggctgt tcctgaacga ttcgttcgta ccaatttgca caagattcca
      721 gaggctcttg cctatgaaaa ggccgtactc acagagcctc tggcctgtgt cttgtttgct
      781 tgtcaaaaaa tccctgttga gccgggggaa accgtggtgg tgattggcga tgggcccatg
      841 agctttttgt tcaatcaggt tttgcagatg caaaaggctc gtacgattgt gtttggtctg
      901 gacacacaac gtttggaatg gatcaaacag tctggcgccg atcatgtctt tgacagcagt
      961 caagtggatg ttgtgaagac tgtgcagggc ttgacagagg gacacggcgc ggctgttgcc
     1021 atcgaagctg ttgggttgcc ccaaacctgg gaacaagctg tacaactagt ttgcaagggc
     1081 ggacgggttt tgtgttatgg ggggtgtgcc aaaggcaccg aaatgaaact cgacacctat
     1141 cgcctgcatt atgacgaaat cagcattctg gggacgtttc actacacccc ttcagtgatg
     1201 gacgaagcga tcaggttgtt ggcggaaggg cgcgtcaaca cagccattct gactgccgaa
     1261 caaagaactc tcaacgattt gcaaaatata tttttaggca aagatcccaa accggcgctg
     1321 aagtacctga tcagaccatg aataaaacga tccacgatcc ataccctaaa gggcacaagc
     1381 gatccttgtt ccccattagt ttcttctttc cgcaatgaag cgggcgatgg ccctgagcgg
     1441 atcggccctc tcatcaaatg aggtcaaaga cgccaaagcg acttccaaaa ggcgtttggc
     1501 ttcttttttg gcaccatcca aaccgaggag agccgggtaa gtggacttgt tttttgcttg
     1561 atcactgcca atgggttttc ccagagattc atcgccttca atatccaaaa tatcatccac
     1621 aacctgaaag caaagaccaa tggcctcgct gtatcgaatc agacagtcca taacgtcggg
     1681 ctcagcgccc ttgagcacag ctggacttgt gacggccagg gtaatgaggg ctcccgtctt
     1741 gtgtctgt
//
LOCUS       DATDUN010000471         1038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_438575, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000471 DATDUN010000000
VERSION     DATDUN010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438575"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VJC18_06590"
     CDS             <1..279
                     /locus_tag="VJC18_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45087.1"
                     /translation="IWSDLAIRPEHVVLVDDKHHGESPMIEHVREIECKAFEVAAKTR
                     NLEDFPKQLVLSEHDAGSDPKLMNEAGLHFSGVFGIEDVHALARQQVP"
     gene            complement(344..>1038)
                     /locus_tag="VJC18_06595"
     CDS             complement(344..>1038)
                     /locus_tag="VJC18_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HLD45088.1"
                     /translation="VGGRYSVLSAVGLFPLALSGVDIKHLLAGARDAVNDCTNKSLKT
                     NRALCSAIVSFINYTKGHSVHNTFLFAPELESLGKWYRQLMAESLGKDGKGLAPLVSI
                     GSTDMHSLAQLFYGGANNAITTIVTAPANGSVKVPKKLIFPGLVEHLEGKSLEELMNA
                     ISTGTTTTYKKLKRPVVSIAFEKLNAYELGYYLQFRMIEIMYLAKLMDVDAFDQPMVE
                     GYKKETKVLLKK"
BASE COUNT          258 a          254 c          250 g          276 t
ORIGIN      
        1 atctggtctg atctcgccat tcgtcccgaa cacgttgtgt tggtggatga caaacatcac
       61 ggggaatccc cgatgatcga acatgtgcgt gagatcgagt gcaaggcctt tgaggtcgcg
      121 gccaaaacgc gcaacctcga ggactttccc aagcagctcg tcttgagcga acatgatgcg
      181 ggaagtgatc ccaagctcat gaacgaggcg ggtctccatt tcagtggcgt cttcggcatc
      241 gaggatgtcc acgctctggc acgtcagcag gttccgtgaa gctcaacggc tcggtccaga
      301 cgggatcgag ttttttcttt ggtaaacaaa aatagccctt cggctatttt tttaataaca
      361 cctttgtttc ttttttatag ccctccacca tcggctggtc aaaggcgtcc acatccatca
      421 acttggcaag atacatgatt tcgatcatgc ggaactgtag gtagtaacca agttcatagg
      481 cattcagctt ttcaaacgca atgctcacaa ctggacgttt gagttttttg tacgtggttg
      541 tcgttccggt agaaatggcg ttcatcagct cctcaagcga tttaccttcg agatgttcca
      601 caagtcctgg aaagatcagc ttctttggaa ccttcacaga cccgtttgct ggagctgtca
      661 caatggttgt gatggcattg tttgcacctc cataaaagag ctgtgccaga gagtgcatat
      721 ccgtggatcc aatcgataca agaggcgcga gtccttttcc atccttaccc aaactttcgg
      781 ccatgagttg tcgataccac tttcctaatg attcaagttc tggagcaaac aaaaatgtat
      841 tgtggacgga atgtcctttg gtatagttga taaacgacac aatcgctgaa cacagggccc
      901 tatttgtttt gagggatttg tttgtgcaat cgtttacggc atctcgtgca cccgccaaga
      961 gatgtttgat gtcgactcca gagagcgcaa gagggaagag accaaccgca ctcaatacag
     1021 aatagcgtcc accaacca
//
LOCUS       DATDUN010000472         4118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_106483, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000472 DATDUN010000000
VERSION     DATDUN010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106483"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1194
                     /locus_tag="VJC18_06600"
     CDS             175..1194
                     /locus_tag="VJC18_06600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLD45089.1"
                     /translation="MAGGPVTQVRNMLTGFNAVFALDVGIDTGLVDNLAAQEPVSLER
                     FVNSTEMHPRHVGAWLRAVQAAGVVEVDKDGMARFSPGWRDALTNPDSPDYVRTLPGC
                     YAAVAGVAYPRFAGLFKSQETMDWRDFPKDVIKKVYADGARFGREFADEVVPVIPGLK
                     ARLESGIMVYDVGCGDGGFLFRFAEKYPRSNCLGIDCCQAAIDLANEGKSKIGRQTKI
                     QFTTQMIHELPEAQADLIVLSEVLHEVGADKRGDLLQAIYRTLKPGGVLFVVDPVLPD
                     SHEGYLTRSAQHLAMMVFSEAPLVPDAPLTRKDFFALLKAAGFDQSLNEVGSSDSYLT
                     AWVVK"
     gene            1219..1380
                     /locus_tag="VJC18_06605"
     CDS             1219..1380
                     /locus_tag="VJC18_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45090.1"
                     /translation="MKKENAKKTNNKKDVLQLRKSNLKTLKRVLVIGAVTIAVSALPT
                     IAMGCADRM"
     gene            1383..2552
                     /locus_tag="VJC18_06610"
     CDS             1383..2552
                     /locus_tag="VJC18_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD45091.1"
                     /translation="MKRLYPFYGPTQKYFYDSAENHVLDARRNQKPAGQQADRGCPAG
                     LGSHIVFLELTKKCNLRCVYCYQSREAFAIGPEASEEVKFAVLTNRLEHLWKRMKSTH
                     LVFYGGEPLLCKSLIKRITDYCDHVMVESSVRCTYAIVTNGTLLEPDINRFFIDRKFS
                     ISLSIDGDAETMKINRSVASLDSIESSMRQLAREGLPLRLIATLQSSNMSRCRQNVDY
                     LLQLPVQSISFIPCETFCTKLNLSVQDAKIFCAVLKQKVHGLLVEQRFEELHKLTELM
                     ALIRKIDSQKRVQCRCGYGKDVFGLSQQGSLYPCPSFIGDVAFEIKSDEQVSFPKKAQ
                     MKCASCLAESLCGGHCQLANYMATTAPSSSILARCYINRKLTQLALDVYAKLYLD"
     gene            2545..3726
                     /locus_tag="VJC18_06615"
     CDS             2545..3726
                     /locus_tag="VJC18_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLD45092.1"
                     /translation="MIKHLQPVAWSLLRTTVVLIVFFGLLFPKMVFADQPTTYRIALV
                     HTFSKADPNCYDPYGKNLENGVRLAWEVFKRTHHGLSFEIEFKQYDLADNPLLAAPRV
                     MEAAQDSSVIAAIGLGCSDLALLGGVEAQHQGLPILTPGATDDRVSNIGDNVFMILFS
                     NSYQGEVLAQFAFHELKKKDVLVIKAVDCTYCLSLADAFKVSFEKQGGHVVADLNVLK
                     TDQDFKDLVRESKKYKFDVVLLANYAMQVAGIIGELVKEGTDIPFLGGDSWLFTKESF
                     EAAGDIPFVGYSSTVWVPDYPTPNSKKFVLEYSKKFKEPLLDTAAYSYDAAMLIFNAL
                     QKTATPTRENLRQALLATGSYEGVSGTMNYGGKTVPKRGALIMKTTNHKQQIYKVFES
                     N"
     gene            3772..>4118
                     /locus_tag="VJC18_06620"
     CDS             3772..>4118
                     /locus_tag="VJC18_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45093.1"
                     /translation="MLFVCMLFFAHFQYNRVFNRARDAEQVNNLVAVTQNDTNLLLAN
                     LILKQDAAIEIYLDRIKKRDDLVAATIIPTGAAEQEARLIVSCGKEVFEKQGYCFVKE
                     NRNRIFYALLRYG"
BASE COUNT         1073 a          808 c         1068 g         1169 t
ORIGIN      
        1 aatgcagttc gcgaatcgga cagttcgata gtctctgcgg atcaggtggc acaaattaca
       61 gaagaaatga accagtatct cagggaatat ggagaaaaaa gtttgtttaa ttatccacat
      121 tatttggtag ttggacaaaa aaattgagac cttaacaaaa caaagggggt ttttatggct
      181 ggtggtcctg tcacgcaagt gcgaaacatg ttgacgggtt ttaatgccgt gtttgctctc
      241 gatgtcggca ttgatacagg attggtagac aaccttgccg cacaggaacc tgtgtcatta
      301 gagcgatttg tcaacagcac cgaaatgcac ccacgccatg tgggcgcttg gttgagagct
      361 gtgcaggcag caggggttgt ggaggttgac aaagacggta tggcgcgttt ttctcctggc
      421 tggcgggacg cccttaccaa tccagattct ccggactacg tgaggacttt accgggctgt
      481 tatgcggccg tggctggtgt agcgtatccc cgatttgcgg gattgtttaa gtctcaagaa
      541 acaatggatt ggcgggattt tccaaaagac gttatcaaaa aggtttatgc tgatggggcg
      601 cgctttggtc gtgaatttgc agacgaggtt gttcccgtaa ttcctggtct aaaggctcga
      661 cttgagtctg gtatcatggt ttacgatgtg ggttgcgggg atggtggatt tttgtttcga
      721 tttgcagaaa agtatccgcg ttcaaactgt ttgggcattg attgttgcca agcggccatc
      781 gatttagcca atgaaggaaa atcgaagata ggccgccaga caaagattca atttaccaca
      841 caaatgattc atgaactacc tgaagcgcag gcagacttga ttgtgctgag cgaagtgttg
      901 cacgaggtcg gagcggacaa acgcggtgac ctgttgcaag ccatttatag gaccttgaaa
      961 ccgggtgggg ttttgtttgt tgtggaccct gttttacctg attctcatga ggggtatttg
     1021 acacgatccg cacaacactt ggccatgatg gttttttcgg aggcgccact tgttcctgat
     1081 gccccgttga ccagaaaaga tttttttgcg ctgttaaagg cggcaggttt tgaccaatcg
     1141 ctgaatgagg ttggatcatc ggattcctat ttgacagcgt gggtggtaaa ataagaacct
     1201 atcaattaag gagattgtat gaaaaaagaa aatgccaaga aaacaaacaa caaaaaggat
     1261 gtcctgcaac tgagaaaaag caaccttaaa accctaaaaa gggtcctagt catcggtgcc
     1321 gtgacgatcg cggtttctgc attgccgacc attgccatgg gctgtgcgga cagaatgtga
     1381 ccatgaagcg gttatatcct ttctatggcc caactcaaaa gtatttctat gattccgctg
     1441 aaaatcatgt tttagatgct cgacggaatc aaaagcctgc cggacagcag gctgatcgtg
     1501 gttgtccggc aggtttgggt agccacattg tttttttgga actgaccaaa aaatgcaatt
     1561 tgcgatgtgt gtattgctac caatcgagag aagcctttgc cattgggccg gaagcttccg
     1621 aagaagtcaa gtttgccgtc ttgaccaatc gcttggaaca cctttggaaa cgaatgaaaa
     1681 gcacgcatct cgtgttttat gggggcgagc ccttgctgtg caaaagtctg atcaaaagga
     1741 taacggacta ttgtgatcac gtcatggttg agagcagtgt gaggtgcact tatgccattg
     1801 tgacaaatgg cacattgttg gaaccagaca tcaacaggtt ttttatagat cgaaaatttt
     1861 ccatttctct cagcattgat ggtgacgcgg agaccatgaa aatcaatcgg tctgtggcga
     1921 gtttggactc catcgaatca tctatgcggc aattggctcg tgagggtttg ccgttgcggc
     1981 tcattgctac cctgcaaagc tccaatatgt ctcgttgcag acaaaatgtg gattatctgt
     2041 tgcaactccc ggtccagtct atttcattta ttccttgtga aactttttgc accaagctca
     2101 atctttccgt gcaagacgcc aaaatctttt gtgctgtctt aaaacaaaaa gtgcatgggt
     2161 tgctggtaga gcaacgtttc gaggaattgc acaaattgac ggaattgatg gcattgatca
     2221 gaaaaattga cagtcaaaaa cgggtgcagt gccgctgtgg ttatggtaag gatgtttttg
     2281 gcctgtccca acagggttcc ttgtatccct gcccatcctt tattggtgat gttgcttttg
     2341 agatcaagag tgacgagcaa gtgtcttttc ccaaaaaggc acaaatgaag tgcgcgagtt
     2401 gtcttgccga aagtctttgt ggtggccatt gtcaacttgc aaattacatg gcaacaacag
     2461 cgccgtcgag ctccattttg gctcgctgtt atatcaacag aaaactgaca caattggccc
     2521 ttgatgttta tgcaaaactg taccttgatt aaacatttac aaccagtggc atggtcttta
     2581 ctcagaacaa ccgtggtgtt gattgtgttc tttgggttgt tgtttccaaa aatggttttt
     2641 gcggatcaac ccacaaccta tcgcattgcc ctcgtgcaca cgttttcaaa ggcagaccca
     2701 aactgctatg acccttatgg caaaaacctt gaaaatggtg tgcgtttggc ttgggaggtt
     2761 tttaagcgaa cgcatcatgg gttgtctttt gaaatcgaat ttaagcagta tgatttggct
     2821 gacaacccgt tgttggcagc accacgcgta atggaggcgg cacaagatag cagtgtgatt
     2881 gcggccatag ggcttggttg ttctgatttg gctcttttgg gaggcgttga ggcgcaacat
     2941 caagggttgc ccattttaac gccgggcgct acggatgaca gggttagtaa cattggcgac
     3001 aacgtgttca tgattttgtt ttccaattct tatcagggtg aggttttggc ccagtttgcc
     3061 ttccatgaat tgaaaaagaa agatgtgctg gtaatcaagg ctgttgattg cacttattgt
     3121 ttgtcattgg ctgatgcctt taaggtgagt ttcgaaaaac agggggggca tgttgtggct
     3181 gatctgaatg tgcttaagac agatcaggac tttaaggatc ttgttaggga gagtaaaaaa
     3241 tacaaatttg atgtggtttt gttggccaat tatgccatgc aggttgcagg catcattggt
     3301 gagcttgtta aagaggggac agacattcct tttttgggtg gggattcctg gctttttaca
     3361 aaagagtctt ttgaggcggc tggtgatatt ccctttgttg gttattcttc cacagtatgg
     3421 gtgccggatt atccaacacc caattcaaaa aagtttgttt tggaatattc gaaaaaattc
     3481 aaggagccgc ttcttgatac tgcggcatac agttacgatg cagccatgct gatttttaat
     3541 gccctgcaaa aaaccgcaac accaactcga gagaatttga ggcaggcgct tttggccaca
     3601 ggcagttatg agggggtgag tggcacaatg aactatggtg gcaaaactgt tccgaaaagg
     3661 ggtgccctca tcatgaaaac caccaaccac aaacagcaga tttacaaggt gtttgaatca
     3721 aactgaatga aacttattaa taagatcaat ctgattctgt tttgtgtttt tgtgcttttc
     3781 gtatgtatgt tgttttttgc ccatttccaa tacaacaggg tatttaaccg tgctcgggat
     3841 gcggagcagg tcaacaacct ggtggccgtg acccagaacg atacgaactt gttactggca
     3901 aatttgattc tcaagcagga cgccgccatt gagatttatc ttgatcgcat caaaaagaga
     3961 gatgaccttg ttgccgcaac gatcattcca acaggtgctg cggagcagga ggcgcggctc
     4021 atagtaagtt gcggcaaaga ggtttttgaa aaacagggtt actgttttgt gaaggaaaat
     4081 cggaacagga ttttttatgc gttgcttcga tatggcaa
//
LOCUS       DATDUN010000473         2509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_479597, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000473 DATDUN010000000
VERSION     DATDUN010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479597"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VJC18_06625"
     CDS             complement(<1..585)
                     /locus_tag="VJC18_06625"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HLD45094.1"
                     /translation="MLSHRDLLHNASSNHLFLILGYGMTGQALARFLESQKRRYVIFD
                     EKDIKTPSSPYCLDRIDLKQGSQLLLENKISAIFPSPGVSLQHTLNTLACQSKVPIIG
                     ELELASFYLKGDFIAVTGTNGKSTTAMLISALLKDAGVENRLSGNIGAPLIDAVNEPP
                     FPYYVIEESSYQLELVGTLRHHIAICLNITEDHFD"
     gene            complement(587..1663)
                     /gene="mraY"
                     /locus_tag="VJC18_06630"
     CDS             complement(587..1663)
                     /gene="mraY"
                     /locus_tag="VJC18_06630"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HLD45095.1"
                     /translation="MFYHLLYPLSDDLILFNVFKYITFRTFGALVTAMVVYFLLGNKW
                     IRFLRKKQFRQVIRQDGPITHLKKSNTPTMGGILVVGCLFLSSLLWCDLSNRFVWASL
                     FIMLGFALVGFVDDYQKIIRKNTQGFRGQYKIIIEVALCLCVGLFLYGNGALDTLLHF
                     PFFKSFTPDIGVCYLFFTVLVVAGTANAVNLTDGLDGLVTVPTAIAFFSYGILAYAAG
                     NVIISNYLQVPNVPGVGELSIISGAVIGSLIGFLWHNSHPAEVFMGDVGALGLGGLLG
                     MMALMTKNEFLLIPIGGIFVLETVSVIIQVASFKLTGKRVFQMAPIHHHFELKGWKES
                     KVIVRFWIIAIICSLFALVTLKLR"
     gene            complement(1668..>2509)
                     /gene="murF"
                     /locus_tag="VJC18_06635"
     CDS             complement(1668..>2509)
                     /gene="murF"
                     /locus_tag="VJC18_06635"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HLD45096.1"
                     /translation="LITSIGQAHLEGVGGTLEKVAQAKGELFDGLEPSATAIYFADDP
                     LIAGLPTRAQKITFGFEPTRDVWASDITLQGNQTRFIVRQDGQETPITLNLVGKHHVQ
                     NALAVFTLARHGGLAPDSIQNGLNSFRMTLNRGKIISVGKIILIDDTYNANPDSMKAA
                     LKSIAEQYPHHVKVAVLGGMLELGKNAAKLHSQVGKFAKEIGVKELFVYGQNSPFYLR
                     SYGYKKEEENEHFFRGHSDLARAVIMCLQNKSDQKTVVLFKGSRGMTMEKALEHVKVL
                     LAK"
BASE COUNT          631 a          613 c          660 g          605 t
ORIGIN      
        1 atcgaagtga tcctcggtga tgttgaggca aatggcgatg tggtgcctga gcgtgcccac
       61 cagttcgagc tggtagctgc tttcttcaat cacgtaataa gggaaggggg gttcgttgac
      121 cgcatcaatc aaaggagcgc caatatttcc gctgagcctg ttttcgacac ccgcatcctt
      181 taacaaggcg cttatcagca tggcggtggt cgatttccca ttcgtccccg tcacggcaat
      241 aaaatcacct tttaagtaaa aagatgccaa ttccaattcc ccaatgattg gaactttgct
      301 ttggcaggcc agcgtgttga gcgtgtgttg caacgaaacc cccgggctgg ggaaaatcgc
      361 agagatttta ttttcgagaa gcaattgtga gccctgttta agatcgatgc gatccagaca
      421 gtaggggctg gagggcgttt tgatgtcttt ttcgtcgaag atgacgtacc gtcgtttttg
      481 tgattccaaa aaacgagcca gggcttgtcc tgtcatgcca taacccagaa tgagaaacag
      541 atgatttgat gaggcgttgt gtaacaggtc tcggtgtgaa agcatattat ctcaacttca
      601 gtgtcaccag ggcgaagagc gagcagatga tggcaatgat ccagaagcga acaatcacct
      661 tggattcctt ccagcccttg agttcaaaat gatggtgaat aggtgccatc tgaaaaacgc
      721 gcttgcccgt taatttaaaa gaagccacct ggatgatgac cgacacggtt tccaatacaa
      781 agatgccacc gatcgggatc aacaaaaatt cgtttttggt catcagggcc atcatcccca
      841 gcaaaccgcc gagtcccaag gcccccacgt cccccatgaa cacttcagca gggtgagaat
      901 tgtgccacaa aaaaccgatg agtgagccaa tcacggcccc gctgatgatg ctcaactcgc
      961 cgacgccggg cacgttgggc acctgcagat aattggagat gatcacgttg cccgccgcgt
     1021 acgcgaggat gccgtaacta aaaaaagcga ttgccgtggg gacggtcacc agaccgtcca
     1081 agccgtcagt gaggttgacg gcattggctg tgcccgccac gaccagcacg gtgaaaaaca
     1141 aataacacac gccgatgtcg ggtgtgaagg atttgaaaaa gggaaagtgc aagagtgtgt
     1201 ccagggcgcc gttgccgtac aggaataacc ccacgcacag acagagagcg acttcgatga
     1261 tgatcttgta ttgtccccga aacccctgag tgtttttccg gatgattttc tgatagtcat
     1321 cgacgaatcc caccaaagcg aagcccagca tgatgaagag gctggcccac acaaaacgat
     1381 tgctcaaatc acaccacaac agcgacgaca ggaaaaggca acccaccacc agaatcccgc
     1441 ccatcgtggg tgtgttgctt tttttgaggt gcgtgatggg tccgtcctgg cggatgacct
     1501 ggcgaaattg tttttttcgc agaaacctga tccatttgtt gcccaatagg aaatacacga
     1561 ccattgcggt taccagggcg ccaaaggtgc gaaacgtgat gtacttgaac acgttgaaca
     1621 gaatcaagtc gtcgcttaag ggatagagta agtgataaaa cataatgtta ttttgctaat
     1681 aaaaccttca cgtgctccag cgctttttcc atggtcatgc cgcgcgaacc tttgaagagc
     1741 accaccgttt tttgatctga tttattttgc agacacatga tgacagcccg ggccagatct
     1801 gagtgtccac gaaaaaaatg ttcgttttct tcctctttct tgtagccata gcttctaaga
     1861 taaaaggggc tgttttgtcc atagacaaac agctctttca caccgatttc tttggcaaat
     1921 ttaccaactt ggctatggag tttggcggcg ttttttccga gctccaacat cccccccagg
     1981 acggcaactt tgacgtgatg ggggtactgc tcggcgatcg atttcagcgc ggccttcatg
     2041 gagtcggggt tggcgttgta ggtgtcatct atcagaatga tcttgccaac cgaaatgatt
     2101 ttgccgcggt taagcgtcat gcgaaaactg ttcagaccat tttgaataga gtctggggcg
     2161 agccccccat gtctggcgag ggtgaacacg gccagggcat tttgcacgtg atgcttgcct
     2221 acaaggttga gcgtgatggg cgtttcttgt ccgtcctgtc gcacgataaa gcgcgtttgg
     2281 ttgccctgta gggtgatgtc acttgcccaa acatcacgtg tgggttcaaa accgaaggtg
     2341 atcttttggg cacgagtggg gagtccggca atcaaggggt catcagcaaa ataaattgct
     2401 gtggcagagg gttcaagtcc gtcaaagagt tctcccttgg cttgggccac tttctcaagg
     2461 gtgccgccca caccctcgag gtgggcctgt ccgatactgg tgatgagac
//
LOCUS       DATDUN010000474         1145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_396003, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000474 DATDUN010000000
VERSION     DATDUN010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396003"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..207
                     /locus_tag="VJC18_06640"
     tRNA            135..207
                     /locus_tag="VJC18_06640"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:168..170,aa:Ala,seq:cgc)
     gene            complement(309..701)
                     /locus_tag="VJC18_06645"
     CDS             complement(309..701)
                     /locus_tag="VJC18_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017026451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLD45097.1"
                     /translation="MNHRGFTLIELILVIAILGILAVSAMPKIFSLTNEAKIAASAGV
                     VGNVIAGITLYGAKQMASTGTESYPATLDGASPGWCTATNLCFTNVVKDGVAHYWTKN
                     DAAIHYTDPTGQTYEYSSIDGTFLPGAI"
BASE COUNT          343 a          271 c          251 g          280 t
ORIGIN      
        1 cccagcgacc gattggaagc gagccgaggc gggggagcgg gcagatgaca ttcaggaatc
       61 tgcctgcgac caaagtcgca agactttttc gttgagacag cagataagtt cgagacaggc
      121 aacagacata ttttggggat gtagctcagt tggtagagcg gttggttcgc aatcaacagg
      181 ccaggggttc gatccccctc atctccacaa caacaaggct cttcccacac aatgatgggt
      241 aagagccttt ttcttttcac cattcgaact caaccttgat cttaagagca tctctaaacc
      301 gatcttattc agatcgcgcc cggcaggaag gttccatcaa tgctggagta ctcatatgtc
      361 tgtcccgttg gatctgtata atgtatggcg gcgtcgttct tggtccaata atgggcaacg
      421 ccgtctttca caacgttagt gaagcacaga ttggtcgcgg tgcaccatcc aggagacgcc
      481 ccgtcaagag tcgcaggata ggactcagtg ccggttgatg ccatctgttt tgcgccgtac
      541 agcgtgatgc cagcgatcac gttgccaaca acaccagcgg aggccgcgat tttggcttcg
      601 ttggtcaagc tgaagatctt gggcatggcg gagactgcca agatacctaa aatagcgatc
      661 acaaggatca attcgatgag tgtaaaacca cgatgattca taaattaccc ctttgtttcg
      721 atctgttaac actcaacagc aggccccccc tgatattgag tcaacaaact gaatacccat
      781 attttacaga aaaaaaaggt tgtcgacaat agaatatgtg tgatgtgcag aatttcgtag
      841 gcaaatttcg ttgagacaac acctttggaa tgcctcaact acataaccct gcaagtcacc
      901 ttgtgatacc tgacaaagct cgcgatgaca aatcgatttc aaaataaata ttcgatccaa
      961 atatatctta aaaaaataca ccatattcct ttcgtaatca aaaaataaac ccaatcattc
     1021 cagcgaacta ttattccccc tcctttggca tgccaattga agtgtaagac tgcagtgaca
     1081 atttaaatat aaacaaaatg gaaaaagggg aaattatgaa aaaaacaatc ttcacactca
     1141 tggcg
//
LOCUS       DATDUN010000475         1747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_272740, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000475 DATDUN010000000
VERSION     DATDUN010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272740"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VJC18_06650"
     CDS             <1..880
                     /locus_tag="VJC18_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HLD45098.1"
                     /translation="GRIPTFVAGEVLAGTTRSEYQAAFDSIRRALSQGDYYELNYTLE
                     FSSPCQGLSWPLYLALREKMKAPMMAYIDFSDLVICSASPERFFRTCGDVISAFPVKG
                     TSPRGQTQEEDRQNLLRLENSEKDRAELLMITDLLRNDLGRVCRVGSVRVETLVQLQT
                     FSHYHHLVSEISGTLLPESSLLDVFSALFPCGSITGAPKIKVMQHTDTLEGRARGVYT
                     GAIGMISNNGDTDFNIPIRTIVRNKNEIHFATGGGIVADSACDSEYQECLTKASGLLE
                     TLAVFGSTAPAFAHQK"
     gene            991..1278
                     /locus_tag="VJC18_06655"
     CDS             991..1278
                     /locus_tag="VJC18_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45099.1"
                     /translation="MVVILNRQFLPENLVASSGQTIFFSNLDDTPHHILSQSGINLFD
                     DTGLFDTDIIPENGATLIVIPEDVSAGGLLYYYDDIMRGEMHTPTGTITIK"
     gene            complement(1285..>1747)
                     /gene="dnaN"
                     /locus_tag="VJC18_06660"
     CDS             complement(1285..>1747)
                     /gene="dnaN"
                     /locus_tag="VJC18_06660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HLD45100.1"
                     /translation="FEFCVSDRHVFIQSEKQTLFIRLIEGNYPDYHRVIPKENPNTIS
                     IPRAEFMGALKRVSLLANDNTKGVSLYFCNDNLSVSTSNPEIGEAKEDIAIKYSGPVF
                     NIGFNARYFIDVLGVVHDENIEISFKDELSPCLVTNPSDPGFQSVIMPMRM"
BASE COUNT          443 a          423 c          399 g          482 t
ORIGIN      
        1 tgggcgcatt cctacttttg tcgcggggga ggttctggcg ggaaccacgc gctcagaata
       61 ccaagcggcg tttgattcaa tcaggcgcgc cctgagccag ggcgattatt acgaactcaa
      121 ctacacgctc gaattttctt caccctgtca gggtctttct tggccgcttt atctcgcctt
      181 gagagaaaaa atgaaggccc ccatgatggc ctatattgac ttctcggacc tcgtcatttg
      241 ctctgcctcg ccggaacgct tttttcgtac ctgcggcgat gtcatctctg cctttcctgt
      301 caaaggaacc agcccccgtg gccaaacaca agaggaagac cgacaaaacc tgttgcgcct
      361 ggaaaacagt gaaaaggaca gggcggagct cctcatgatt actgacctgc tccgaaatga
      421 tttgggacgt gtttgccggg tggggtccgt tcgcgtcgag accttggtgc aactgcaaac
      481 cttttcccat tatcaccact tggtttccga gatctccggc acactgttgc ctgaatcctc
      541 tttgcttgac gtctttagtg cgctgtttcc ctgtggttcg attacggggg cgccaaaaat
      601 aaaagtcatg caacacacgg acacgctgga agggcgcgca aggggcgttt atacgggggc
      661 cattggtatg atcagcaaca atggggacac agactttaat attcccattc gtaccatcgt
      721 gcgaaataaa aatgagattc attttgccac aggtggtggt attgtggcag acagtgcttg
      781 tgattcagaa tatcaggagt gtttgaccaa ggcatcgggg cttttggaaa cgctggctgt
      841 ttttggatca acagcccccg cttttgctca tcagaaatga ccaataaaat tcgcaaagca
      901 accctcttct cattatttta ctgccttccg ttcgttcttc ttttggcttg tggccctgtg
      961 cctgtgtcaa ccgaggtagc agacgccaac atggttgtta ttttaaaccg acagtttttg
     1021 ccagaaaacc ttgtggcctc gtcggggcag acaatttttt tttccaattt agatgatacc
     1081 ccccaccaca tcctaagtca gtcggggatc aatctttttg atgacacggg tctgtttgat
     1141 acagacatca taccagaaaa cggggccacc ctcattgtta tcccagagga tgtgagtgct
     1201 gggggtttgc tgtactatta cgacgatatc atgaggggtg aaatgcacac gccaactggc
     1261 acgatcacaa taaaataatt tctctcacat tctcatgggc ataataacag actgaaagcc
     1321 ggggtcggag ggattggtta ccagacaggg agataattca tctttaaagg atatttctat
     1381 attttcatca tggacaaccc ccagaacatc aataaaatac cgcgcattaa aaccaatatt
     1441 gaagacaggt ccagaatatt tgattgcgat gtcttctttg gcctctccga tttccggatt
     1501 tgatgtgctc acagataaat tatcattaca aaaatagagt gaaaccccct tcgtattatc
     1561 gtttgccaac aacgaaaccc tcttcagggc tcccataaac tctgccctgg gaattgaaat
     1621 tgtatttgga ttttctttgg gaatcacacg atggtaatcg ggataattgc cctctatcaa
     1681 tcgtataaaa agtgtttgtt tttcactctg aataaaaaca tgcctgtcac ttacgcagaa
     1741 ctcaaac
//
LOCUS       DATDUN010000476         4386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_312851, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000476 DATDUN010000000
VERSION     DATDUN010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312851"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..617
                     /locus_tag="VJC18_06665"
     CDS             219..617
                     /locus_tag="VJC18_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLD45101.1"
                     /translation="MNNKGFTLIELILVIAILGILAVSAMPKIFSLTTEANTAAAAGV
                     AANVNSGITLYGAKQMASTGIESYPAALDGKGDGSACDAAFPCFVTVVKDGVSRDWTK
                     VDAFHYRHTPTGIDYTYNNVTGTFQISVGP"
     gene            complement(913..1272)
                     /locus_tag="VJC18_06670"
     CDS             complement(913..1272)
                     /locus_tag="VJC18_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HLD45102.1"
                     /translation="MERPLIHRARELRREQTDHEAELWQFLRAKRFLGLKFYRQYVIE
                     PYIVDFCCPEKSVVIELDGSWHLEPARQKRDQKRDHFIQTQGYRLLRIWNNEWRENKT
                     AVLEMMGEFISAKAPSP"
     gene            complement(1323..2750)
                     /locus_tag="VJC18_06675"
     CDS             complement(1323..2750)
                     /locus_tag="VJC18_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HLD45103.1"
                     /translation="MVVENIDVTHTLEKAKRILDEEKDLSPAVKSIFEVLILLITLMA
                     NRLGRNSKNSSLPPSQDPNRARKNKVAGEIQKRKQGGQENHEGCTLEKSKTPDTIEEI
                     VIDLRTIPAGRTYTVAGYESRQVVDVNINVHITEYRAEILEDETGKRYVARFPDGVNK
                     AIQYGSGVKAESVYLSMFQLVPLARVQEHFADQMGLPISKGSVSNFNQETSERLDAFG
                     TWAKQKLLASPLIHADETGINVGGKRKWLHNLSNEKVTLYHPDEKRGTEAMNRMGVLP
                     LYTGRLCHDHMKSYYKYPNITHVLCNAHHIRELKRVIEEDGHQWAKTLLQFLVDLNQA
                     VDAADGVLSDEKVCVATKQYREILEQGEAECPKPAPKESGKRGRVKKTFSRNLLERLR
                     DFESDTLLFMKEAVVPFTNNQGERDLRMTKVQQKISGCFRSMEGAQVFSRVRSFLSTC
                     KKNGVSPTEALRDLFQGKFPDFMTL"
     gene            3412..>4386
                     /locus_tag="VJC18_06680"
     CDS             3412..>4386
                     /locus_tag="VJC18_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45104.1"
                     /translation="MRTYAMIILVLIFLVPASVLAAGPMINYGDTSGDTIVSMPPVWD
                     NSAPIEYTIESGTCGPFSNDEMVEKYEDILAVWSDIEGVDISFSAVTGEIGSIDDSNY
                     ADYFYYGLEGEDVTVATDGINPVMFDDDGALTDALFGTGGSSSVLGFASVTGLDDDAL
                     YDGEVVINCKCMEDHPTENCDEVVFSEEDLSFTITHEMGHFLNMGHSQANYNLMIDWA
                     ADDFDEATKPFGDGDLPIMFPTSSDMTEVIPQADDVLTLKQLYPSGSLSDDYCKVEGY
                     IVDSEGEYVPCLDVWAVTEDEDPAETLTVTTGSSNTYGTGTGDDFDPDA"
BASE COUNT         1114 a         1027 c         1001 g         1244 t
ORIGIN      
        1 gtcaccctta atcaaatgtt tcgtgttttt ttaagaatct aagcttgaac ccacatttct
       61 cacaaacaat tgtgaaaaat gcattattcg gagagcactt attaatcatt gccacacaac
      121 gctctcatat tgtacaataa cggggttact gctttatgag gtttcaggga gggcctgata
      181 ctgaattaaa gcaaggacat ccaaaaaagg agttacccat gaataacaaa ggttttacat
      241 tgattgaatt gatcctcgta atcgcgatat tgggtatctt ggcagtatcc gccatgccca
      301 agatattcag cctgacaact gaggccaaca cagcggcggc cgctggtgtt gccgccaatg
      361 tgaactcggg gatcactcta tacggtgcca aacagatggc gagcactggt attgagtcct
      421 atcctgccgc cttggacgga aaaggtgatg gctcagcttg tgatgcggcg ttcccctgtt
      481 ttgtcaccgt ggtaaaggat ggcgtgtcga gagattggac caaagtggac gcttttcatt
      541 accggcacac cccaacgggg attgattata cgtacaacaa tgtgacaggc acctttcaga
      601 tatctgtagg gccataaact tatgggctga aattcatcac gacacacgcc cctttggaaa
      661 taccaaaggg gcgtgtcagc tccataaata tttttcgttg tattcaaaat tctgcttttt
      721 caaaaaatcg ataaactcat cctgaaatgt ttttttcctg tgatgctcct gctgattggc
      781 aatgtaggct tcaacaacgt cctgttgaga tttgagttta ttgggttacg atccgtaact
      841 ggtcagcgtg attatattgg gttttaagtg gttggatttt atggaattcg ccctcgccct
      901 ttggggagag ggttagggag agggggcttt ggctgaaata aattccccca tcatttccaa
      961 aaccgcagtt ttgttttccc gccattcgtt attccagatt cgaagcaatc gatagccctg
     1021 tgtctgaata aaatgatcac gtttttggtc tcgtttctgt cgagcgggtt ccaaatgcca
     1081 gctgccgtcg agttcaataa caacagattt ttcgggacaa caaaaatcaa caatgtaggg
     1141 ctcgatgacg tattgacgat aaaattttag acccagaaat cttttcgccc tcaaaaattg
     1201 ccagagttct gcctcatgat cggtctgttc tcgtctgagc tctcgggctc gatgaatcaa
     1261 aggccgttcc atttgatttc tctcctaact tttttcgcgt caaaaaacac aacaagccat
     1321 ttttataaag tcataaaatc aggaaatttg ccttggaaaa gatcccgtaa cgcctcggta
     1381 ggacttacgc cgtttttttt gcaggtggat aaaaaacttc gcacccgact gaaaacctgg
     1441 gctccttcca ttgaacgaaa gcatcctgat attttttgct ggaccttggt catgcgcaaa
     1501 tcacgctcac cctgattgtt ggtaaagggc acgaccgcct ctttcatgaa aagaagtgtg
     1561 tcgctttcaa aatcgcgcag tcgttcgagc agattgcgtg aaaatgtttt tttgactcgt
     1621 ccacgctttc cagactcctt tggcgctggt tttgggcatt ctgcttcacc ctgttccaaa
     1681 atttcacgat actgttttgt cgcaacacaa accttttcgt cagacaaaac accgtcagcc
     1741 gcatccacag cctggttcag atcaacaaga aattgcaaaa gtgtttttgc ccactgatgc
     1801 ccgtcttctt cgatcacccg tttgagctct cgaatatggt gggcattgca gagcacgtga
     1861 gtgatgtttg gatatttgta atacgacttc atgtggtcgt gacagagtcg gcccgtatag
     1921 aggggcagaa cgcccattcg gttcatggct tctgttccgc gcttttcgtc cggatgataa
     1981 agggtaactt tttcgtttga aagattgtgc aaccatttcc ttttgccacc cacattgatt
     2041 ccagtttcat cagcatgaat caacggtgaa gcaagcagtt tttgcttggc ccaagtccca
     2101 aacgcatcga gtcgttcaga ggtctcctgg ttgaagttcg agaccgatcc ttttgaaatg
     2161 ggcaagccca tctgatcagc aaaatgttcc tgcacccgag ccagcggcac cagctggaac
     2221 atcgacaagt agacagattc ggctttcaca ccactgccat actgaattgc cttgttcacc
     2281 ccgtcaggaa atcgtgccac atagcgcttg cccgtttcgt cttccaaaat ttctgcgcgg
     2341 tattcggtga tatgcacgtt gatgttcaca tcaacgactt gtcggctctc gtaaccagcg
     2401 actgtgtatg ttcttcccgc tggtatcgtc cgcaagtcga tcacaatctc ttcgatcgtg
     2461 tctggcgttt ttgatttttc gagcgtgcaa ccctcatggt tttcttgccc tccctgcttg
     2521 cgcttctgga tttctcctgc cactttattc ttacgcgctc gattgggatc ctgtgatggt
     2581 ggaaggctgc tgttcttgct gtttctcccc agtcgattcg ccatcagggt gatcagaaga
     2641 atcagaactt cgaaaatgga tttgaccgcc ggggacaaat ctttttcttc atccaaaatc
     2701 cgtttggcct tttccaaagt atgtgtcacg tctatgtttt caactaccat ggtttcttat
     2761 accacaggtt tcaaaatgga gaaatttcgc ggcagattgt ctcaggagcc tctgaaaaac
     2821 acaggacgtc aacaagttcg ttctgatgat ctttgtggtt ctgaagcgtc tgcaaacaac
     2881 acaggaaggc cttgctgaaa acaacatcgt tcagaaatac aaacacgttt tttgcaaaaa
     2941 aacaacgtca agaaaaatcg tattaaaaat aaaatttatt tttctgacaa cctttttttc
     3001 gccccctctc cctaaccctc tccccaaagg gcgagggcga aatcccaagt cctaccagat
     3061 cacaaatccc caaaaaaacg ctgaccagtt acaaaaaata cgataaaaat aataagataa
     3121 gatgacggag ttgatttgaa tgatttgaaa gccaagggat aaaggatgat taaggattaa
     3181 ggaaaggatt aaggatgacg gagttaatct gaaaagaaaa taacgcaagg cggaggatgt
     3241 cctgtaactc aattatctca tttgttacgg ttggttacaa gatactataa aaaggtacct
     3301 ggcaccttct ggcaccttca aaaatgtacc tggcaccttc ctggcacctt ccttttcttc
     3361 cttttctctt tccatatgca caaagatatc agataataat aatgatatct tatgcgtacc
     3421 tacgccatga tcatacttgt cctcatcttt ttggtccccg cttctgttct ggccgccggg
     3481 ccgatgatca actatggcga cacatctggt gacacgattg tctccatgcc accggtttgg
     3541 gacaattcgg ctcccattga atacaccatt gagtcgggaa cctgcgggcc tttttcgaac
     3601 gatgagatgg tggaaaaata cgaggacatt cttgctgtct ggagcgacat tgaaggtgtt
     3661 gacatcagtt tttccgctgt gaccggtgag ataggttcta tcgacgacag caactacgca
     3721 gattattttt attatggtct ggagggagag gatgtcactg tagccactga tggcatcaac
     3781 cccgtgatgt ttgatgatga tggtgctttg accgatgcgc tatttggtac tggcggttcc
     3841 agttctgtac tgggttttgc ctctgttacg ggactcgatg atgatgctct ctacgatggg
     3901 gaggtggtga tcaactgcaa atgcatggag gatcacccca cagaaaactg tgatgaagtt
     3961 gttttttccg aagaggacct gagtttcaca atcacacatg agatggggca ttttctgaat
     4021 atgggacatt cgcaggccaa ctataacctc atgatcgact gggctgccga tgactttgat
     4081 gaggcaacca aaccctttgg tgatggggat ttgcccatca tgtttcccac ctcgagtgac
     4141 atgacggagg tcatcccgca ggcggacgat gtcctgaccc tcaagcagtt gtaccccagc
     4201 ggctctctgt cagacgatta ctgcaaggtt gagggctaca tcgtggacag tgagggcgag
     4261 tatgttcctt gtctcgatgt ctgggcggtg accgaagatg aagaccccgc tgagaccctc
     4321 accgtgacga cgggcagtag caatacctac ggcacgggaa cgggtgatga ttttgatccc
     4381 gatgcc
//
LOCUS       DATDUN010000477         1085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_479790, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000477 DATDUN010000000
VERSION     DATDUN010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479790"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..>1085)
                     /locus_tag="VJC18_06685"
     CDS             complement(45..>1085)
                     /locus_tag="VJC18_06685"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HLD45105.1"
                     /translation="KRNKVGCEMRNVCYMIFFLLFFSFAAQAGVTEPVASGPFYVRSQ
                     NPLYLKFLAMPMEPASTLVPGVWQNTLSTTFSNVFEYDPVGDTVLNLDMEIWRTAYSF
                     AYGVSKVFDVRVDIAFVSNMGGFLDSFIQGYHDAFGFPNGGRDLVPNNEYHFELTQNG
                     NALVDYSQETFGLSDVVLRGKYFLSKHISLPIDLATAFYFKLPVGRSGKGLSSGQADA
                     GLSLFGQKSFKRFHLVSQLGVVVMGEDALLAPLQKRAQFVFGQSVEYQILDGWSVLAQ
                     LSGNTAAFDRVDTTDLSDPVMDLTLGFAGTFLPKKGRVGELFYQWSFSEDVLSRGPSV
                     DFSMLFLVGVRY"
BASE COUNT          327 a          323 c          210 g          225 t
ORIGIN      
        1 atctcatatc accgcctatc ctcttcccct cattcatcga acgattagta cctcaccccc
       61 accaaaaaca acatcgaaaa atcaacgctc ggaccacgac tcaacacatc ctcactaaat
      121 gaccactgat aaaacaactc accgactctg ccctttttgg gcagaaaagt ccccgcaaaa
      181 ccaagggtca aatccatcac ggggtcactc aggtctgtcg tgtccacacg atcaaacgcc
      241 gcggtattgc cactcaactg cgccagaaca gaccacccgt caaggatttg atactcaacg
      301 ctctgcccga acacaaattg agcgcgcttc tgtaggggtg ccagcagggc atcttctccc
      361 atcaccacca cacccagttg tgaaaccaga tgaaaccttt taaaactttt ttgtccaaac
      421 aacgacaagc ccgcatctgc ctggccgcta ctcaatccct tgccgcttct tcccaccgga
      481 agcttgaaat aaaatgctgt ggccagatca attggcaaac tgatgtgttt cgacaaaaaa
      541 tacttccccc gcaaaacaac atcggagagc ccaaacgtct cttgcgagta atcaaccagg
      601 gcattgccat tctgcgtcag ttcgaaatga tactcgttgt tgggaacaag atctctgcca
      661 ccattgggaa agccaaaggc atcgtgatag ccttggatga acgaatccag aaacccaccc
      721 atattgctga caaacgcaat gtccactcga acatcaaaga ccttcgaaac cccgtacgca
      781 aaagagtacg ccgtcctcca gatttccatg tccaaattta atacagtatc gccaacagga
      841 tcatactcaa acacattcga aaacgtggtg gacagggtgt tttgccacac gccggggaca
      901 agcgttgacg ccggttccat gggcatggcc aaaaacttga ggtacagggg attttgtgag
      961 cgaacataaa agggtccaga ggccacaggc tccgtcacgc ctgcctgagc ggcaaaggaa
     1021 aaaaacaaca gaaagaaaat catgtaacaa acatttctca tctcacagcc taccttgttc
     1081 ctctt
//
LOCUS       DATDUN010000478         3085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_396162, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000478 DATDUN010000000
VERSION     DATDUN010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396162"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1062
                     /locus_tag="VJC18_06690"
     CDS             139..1062
                     /locus_tag="VJC18_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD45106.1"
                     /translation="MDQNKSTADSLNIFSYDDYRNFLADFYSQKKAQQPQQFSFRVMS
                     TKAGFKTPSFIPYVIQGKRNLTLESIEKIAGLVPLKGRPLEYFKTLVMFNQAKSNEDR
                     NRHYDTMKEFKEYVQMRLLTNDQCTYMLKWYYPVVREIVRWPSFRPEPQWVASKIKPR
                     IFVEQAKEALDVLQSLQMISVNPDGSWKQAFPKVGSPDQGVGKEVLSFYDKAILLGRD
                     SLKLPASERNISSMIMSLSPASFELIRYRINEFQKELNGIISQEVTPEHMRAQQVPED
                     IINQKEFFDVSEVGLLNIQFFKVAKAKGYYE"
     gene            1055..2530
                     /locus_tag="VJC18_06695"
     CDS             1055..2530
                     /locus_tag="VJC18_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45107.1"
                     /translation="MNNYTKRLVTIVLLMMAFVFTSCAGLSNPITPSEGDEQIMEDPQ
                     IDSTPPTSSTTLGNPTLDVSVTGNVFLTDNNASDNLKQVTGDDVFSVLSLVDPDVGTE
                     TLTESPTTDIPSVKYFGVGTDSQLVMFFDEAISVADADCYVVYAYEDEQLCLESSQFT
                     IVGDVQFDKAGNIYYFGQENSADVQTLVRWDPETAERKLLINQNITLTQWMVHADGSV
                     YLLGESGGIGFFRKMNTDGSLVNIFSSPTVISQFNFISSELMIAEGDFVYYNDDWSLG
                     LFVISLADFSVKDRILLSDVDLTTLKIDSQNRVFALSGYHLNQKLYQIYPKSIVADSL
                     VNLGLNQIKYFEIINDILYAEGMQDTNEVLVQLDLNLEVAVSGVREATVLCSGYEVYH
                     LIENDGALYFDGLDFANNTMSLFRFDTETLTLSPLVQTSDALVQLLSNKAYENPSFRD
                     RVFEVENIPTESGGAEFIGKKLEDSKFRKPGLSASALKLLD"
     gene            complement(2538..>3085)
                     /locus_tag="VJC18_06700"
     CDS             complement(2538..>3085)
                     /locus_tag="VJC18_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HLD45108.1"
                     /translation="LIGDNTDCEGFVTGLVNETGLTIKDAKILILGAGGAARAICFGL
                     CAAGALSLTIVNRDQNKALPLCRQLTAEFDLVTIEHRPWSVLDNPLPDDLSLIVNATA
                     LGMEDRPWPHLEFVNTCPQLVCVADIVTQDKDTELITAAKHCGLKTMSGLTMLLHQAV
                     LAFELMTGQKAPVEVMRAVQS"
BASE COUNT          811 a          736 c          778 g          760 t
ORIGIN      
        1 aacagagtac agaattgact tgccaaacaa ccccggcgca actagttgac aacacgttga
       61 aattacttgt taaatatcta gcaacttatc acgatttgtg ccgataatat tcgtatgcaa
      121 acaagacaga ggttcgctat ggatcaaaat aagagtacag cagattcgtt aaacatcttc
      181 agttacgacg attaccgtaa ttttttggcc gatttctatt cgcaaaaaaa agcccaacag
      241 ccccagcaat tttcattccg agtcatgagc acaaaggcag ggttcaaaac gcccagtttt
      301 atcccttatg tgattcaggg gaaaagaaat ctcacactgg aaagcatcga aaaaattgcc
      361 gggctggttc cactcaaggg caggccactg gaatatttca aaacactcgt catgttcaat
      421 caggccaaat caaacgagga ccgtaaccgc cattacgata ccatgaagga atttaaagaa
      481 tatgtgcaga tgaggttgct caccaatgat cagtgtacgt acatgctcaa gtggtactac
      541 cccgtggtgc gcgagatcgt gcgatggccg tctttcagac ccgaaccaca gtgggtagcg
      601 tccaaaatca aaccccgaat atttgtagaa caggccaaag aggcgctgga tgtgttgcaa
      661 agcctgcaaa tgatcagcgt gaatcccgat ggttcctgga agcaagcgtt tcccaaggtt
      721 ggttcgccag accaaggagt gggcaaggaa gttctgtcgt tttacgacaa ggctattctt
      781 ttgggacgcg atagtctcaa actgccagcg agtgagcgca acatcagctc catgatcatg
      841 tcactctcac cagcctcgtt cgaactcatt cgctaccgga tcaacgaatt tcaaaaggaa
      901 ctcaacggga tcatctcaca ggaagtgact ccggaacaca tgcgcgcaca acaggttccc
      961 gaagacatca tcaatcaaaa ggaatttttt gacgtgagcg aggtgggctt gttgaatatt
     1021 caatttttta aagtcgccaa agcgaaagga tattatgaat aactatacca aaagactcgt
     1081 gaccatcgtg ctgctcatga tggcgttcgt tttcacttcg tgtgcgggcc tgtccaatcc
     1141 gatcacgccc agcgagggtg atgaacagat tatggaagac ccgcagatcg acagcacgcc
     1201 gccgaccagc agtaccacgc tgggcaaccc gaccttggat gtcagtgtca caggcaacgt
     1261 gttcctcacg gacaacaatg ccagcgacaa tctcaaacaa gtcacgggag acgatgtctt
     1321 cagcgtcctg agtctggttg atcccgatgt gggcaccgaa acgctcacag aatcacccac
     1381 gaccgatatt ccttctgtaa agtatttcgg tgttggaacc gatagccaac ttgtcatgtt
     1441 ctttgacgaa gccatttccg tggctgatgc agattgttat gttgtgtatg cctacgagga
     1501 tgaacagttg tgtctggaga gcagtcagtt taccattgtc ggtgatgtgc aattcgataa
     1561 ggctggcaac atctattatt tcggacagga aaacagtgca gacgtgcaaa cgctggttcg
     1621 ctgggatcct gaaacggctg aacgtaaatt actcatcaac cagaacatca cgctcacgca
     1681 atggatggtg catgcagatg gttctgtgta tcttctgggt gaatcgggcg ggattggttt
     1741 tttcagaaaa atgaacactg atggcagtct ggtcaacatc ttcagcagtc ctacagtcat
     1801 cagtcagttc aatttcatca gctctgagct gatgatcgca gagggtgatt ttgtctatta
     1861 caacgatgat tggagtttgg gtttgtttgt gatttccctg gctgattttt cggtaaagga
     1921 tcggatactt ttgagtgacg ttgatcttac cacgttgaag atcgattctc aaaatcgtgt
     1981 gttcgcgctg tctggctatc atctgaacca aaagctgtat cagatttatc ccaaatcgat
     2041 cgtggcagat tccctggtga acctggggtt gaatcagatc aaatatttcg agatcattaa
     2101 cgatattctt tacgcagaag gcatgcagga tacgaatgaa gtcctggttc agctcgacct
     2161 caacctggaa gtggccgtga gcggggttcg cgaagcaact gtcttgtgtt ctggttacga
     2221 ggtgtaccac ctgattgaga acgatggggc cctgtacttt gatgggcttg attttgcaaa
     2281 caatacgatg tcccttttcc ggttcgatac agaaactctc acattgagcc cgctcgtcca
     2341 gaccagcgat gccttggtcc agttgttgtc gaacaaggcc tatgagaacc cctcttttag
     2401 ggacagggta ttcgaagtcg agaacatacc gaccgaatcg ggtggtgcgg aattcatcgg
     2461 caagaaactg gaagacagca aatttaggaa accgggtttg agcgcctctg ctctgaagct
     2521 gcttgattaa aaattgatta ggattgaact gccctcatca cttccacagg tgctttttga
     2581 cccgtcatga gctcgaaggc gaggacggct tgatgaagaa gcatggtcag gccgctcatc
     2641 gttttgagtc cgcagtgttt tgccgcggtg atgagttccg tatctttatc ctgagtgacg
     2701 atgtcggcca cgcagacgag ttgggggcag gtgttgacaa actccagatg gggccagggc
     2761 cgatcctcca tgcccagcgc cgtggcgttg acgatgagcg aaagatcatc cggcagaggg
     2821 ttgtccaaaa cggaccaggg cctgtgctcg atcgtgacga gatcaaactc cgcagtcaac
     2881 tgcctgcaga gagggagggc cttgttctgg tcccggttga cgatcgtcag ggacaacgcc
     2941 ccggcagcac acaatccgaa acagatggct ctcgccgcgc cgccagcccc cagaatcaga
     3001 atctttgcgt ctttgatggt gagcccggtt tcgttgacca gccctgtcac aaaaccctcg
     3061 cagtccgtat tgtcgccgat caatt
//
LOCUS       DATDUN010000479         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_190253, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000479 DATDUN010000000
VERSION     DATDUN010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190253"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="VJC18_06705"
     CDS             <1..1731
                     /locus_tag="VJC18_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45109.1"
                     /translation="DVNTTTTAGDVSIPSGETTSSEMGWFERQITYYPVDSYVEYFTN
                     NDVPVLQRAISGALAPIVVPLSLAGCNNSKPTDDTGVIGDDDSDYVPQNLCVMSDPVD
                     TDNDDLVDDISVQGDCRLQEAYDRLEDATNTITILSGYGIDSDYPDDNIPTFGNMTVV
                     SGKNLTINADTASGHPVRLEMPETSDDTSAAFNVASGATLDINGNNSLEIYGSGNSTG
                     VSAQSGSVVTIDDTSFENLAYGVVSDGATTTLKNNTFDIIRNIAMGFYNGARAYVYDS
                     AVVGSTTGFAEPKAQYAIDSIDASEIVLRRNYVENTETTGDTIRLDADYTEHFGNILY
                     GNHAEGYLLNWSGGCDNWPSAGQQYHYGSAFMAAENTHGIDGAVNITSACDVLFTNYT
                     IANNYGTFYTPSYVLDISDASNGNQGKITATQGVIYGNDATTLIVKRSEGYDTFSYNA
                     VTDNGAGDPTLVDNLGDYPNYIDEDPLLSGHTMYDGTNFYTYAYHSTNTDLLGSYEAV
                     YPNWYDPQANNPGLMGAFNGRLGNFAIVTADRMCEDFGFADCDAQLQALLDSFGPDAD
                     VSGLEDYVDE"
     gene            complement(1738..>2309)
                     /locus_tag="VJC18_06710"
     CDS             complement(1738..>2309)
                     /locus_tag="VJC18_06710"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HLD45110.1"
                     /translation="NYRKLPQDEDRRYKSRGQLLKNYLTGHQNAFAYLFDVSQQYKEY
                     KPFNLLLGEEDSLYYFCSEQREISEITSPGIYGLSNHLLDTPWPKVEQGKRELEKAVF
                     RDRFDLEDLFAILANQDEVEEEQLPETGLDWRWERALAKVFVSHENYATVSSTVILWG
                     WDNHIELIERTYRSGVDDYQDKAFAFQVA"
BASE COUNT          593 a          624 c          523 g          569 t
ORIGIN      
        1 gatgtaaaca cgaccacaac agcaggggat gtttccatcc cttcaggaga aacaacctca
       61 tccgaaatgg ggtggtttga gcgtcagatc acttactatc ccgtggacag ctatgttgag
      121 tattttacca acaatgatgt gcccgtacta caacgagcca tttccggtgc cttggccccc
      181 attgtcgtgc ctctttcact ggccgggtgc aacaactcca aaccgaccga tgacacaggc
      241 gtcataggcg atgacgactc agactacgtc ccccagaatc tctgtgtcat gagcgacccc
      301 gttgacactg acaatgatga tcttgtcgac gacatctctg ttcaaggcga ttgtcgtttg
      361 caggaggcct atgatcgact tgaagatgcc accaacacca tcactatcct gagcggttac
      421 gggattgact ctgattatcc cgacgacaac atccccacgt ttggcaacat gactgtagtc
      481 tcgggtaaaa atctaaccat caacgccgat accgcctcgg gacatcctgt cagattggaa
      541 atgcccgaaa cctctgatga cacgtccgcc gcgtttaacg tggcgtccgg cgccaccctt
      601 gatattaatg gcaacaactc tttggaaatt tatggctcag gaaacagcac cggggtttct
      661 gcccaatcag gaagtgttgt gaccattgac gacacttctt ttgaaaacct tgcctacggt
      721 gttgtgtctg acggcgccac cacaacgctc aaaaacaaca cctttgacat catccgtaat
      781 atcgctatgg gtttttacaa tggcgccaga gcctatgtct atgattccgc cgtggttggc
      841 agcaccacag ggtttgccga gccaaaagcc caatacgcga tcgattccat tgatgccagc
      901 gagattgttc tcagacgcaa ttatgttgaa aacaccgaga caacaggcga caccatcaga
      961 ctcgatgccg attatacaga acacttcggt aacatattgt atggcaacca tgcggaaggt
     1021 tatttgctca attggtctgg gggctgcgat aactggccct ctgccggtca gcaataccat
     1081 tacggctcag cctttatggc ggcagaaaac acacacggga tcgacggtgc tgtcaacatc
     1141 acatctgcct gtgatgtttt gtttaccaac tacacgattg cgaacaacta tggcaccttc
     1201 tacacaccat cctatgtgtt ggacatatct gacgccagca atggcaacca agggaaaatc
     1261 acagccacac agggtgtgat ctatggcaat gatgccacaa cattgattgt caaacgatca
     1321 gagggctacg ataccttttc gtataacgct gtgacagata acggcgcggg tgacccaaca
     1381 ctcgtggata atcttggcga ctaccccaac tacatcgatg aggatcccct gttgagcggg
     1441 catacaatgt acgacggtac gaatttctac acatacgcct atcacagcac caacacagat
     1501 cttctgggtt cgtatgaagc cgtgtacccc aattggtacg atcctcaagc caataaccca
     1561 ggtctcatgg gcgcctttaa cggacgtctg ggcaactttg ccatcgtgac cgcggaccgc
     1621 atgtgtgaag attttggatt tgccgactgc gatgcccaac tgcaggccct gttggatagc
     1681 tttggtcccg atgccgatgt gagcggcttg gaagactatg ttgatgaata ataagcctca
     1741 cgcaacctga aacgcgaacg ccttgtcttg ataatcgtcc acgccacttc gatatgttcg
     1801 ttcgatgagc tcgatgtgat tatcccatcc ccacaaaatc actgttgagc tgaccgtggc
     1861 gtagttttcg tgtgagacaa agacctttgc caaggcccgc tcccatcgcc agtccaagcc
     1921 tgtttccggc agttgctctt cttccacttc gtcctgattg gcaagaatcg caaacaaatc
     1981 ctccagatca aaacgatcac gaaatacggc tttttccaat tcccgtttcc cctgttctac
     2041 cttgggccag ggggtatcga gcagatgatt gctgaggcca taaataccgg gggacgtaat
     2101 ctcggaaatc tctctttgct cgctacaaaa ataatacaag gaatcctctt cacccaacag
     2161 cagattaaat ggtttgtatt ctttatattg ctgactcaca tcaaaaagat acgcaaaggc
     2221 attctggtgt cctgtgagat aatttttcaa cagctgaccc cgtgacttgt aacgcctgtc
     2281 ctcatcctgt ggcaatttgc gataattgg
//
LOCUS       DATDUN010000480         3372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_480007, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000480 DATDUN010000000
VERSION     DATDUN010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480007"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VJC18_06715"
     CDS             complement(<1..475)
                     /locus_tag="VJC18_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLD45111.1"
                     /translation="MTSVKHLIKENETGRIDLVVQKLTERSRTQVRGLFDHGCVLLNG
                     VVCDNAGERVRASDEVSVHYDKNRNYKELPKKYRSPIFDLIFEDEWILVVDKKAGYLT
                     VPTDHREPNTVVGALCQYMSLGKPKRRLVSIVHRLDRDTSGLLVFAKNEKIAQQIK"
     gene            complement(496..999)
                     /locus_tag="VJC18_06720"
     CDS             complement(496..999)
                     /locus_tag="VJC18_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45112.1"
                     /translation="MKNISPILFVSLVICFFVKQTVLAQTPPLPTQLTPNLFLVTTDK
                     TAYAKGEAVRISVTNHSGKSVRVDSYSNPPCPLEQFLNQQWTTVPSVSCSCDDDVQCN
                     MSEAYIDPKKTYEFSWTQTTSWCVWHGEKSAPAKPGIYRVTCPLNDPLSRENRIDVVS
                     QNFIIKE"
     gene            complement(1118..2626)
                     /locus_tag="VJC18_06725"
     CDS             complement(1118..2626)
                     /locus_tag="VJC18_06725"
                     /inference="COORDINATES: protein motif:HMM:NF037672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector HopA1 family protein"
                     /protein_id="HLD45113.1"
                     /translation="MAPDIPRLSPFATQLALSPEVMGQTVVAKASGFEMASPSLMPKG
                     IEPPVRDLAGQPLLPLLMRLGDFEHVKGRPGQMDDPQAHVDIEHIGGVSLLTPRRPNI
                     PCAIIAPSGNVTVFTGKAMKGMPVVSELRQREGVRITIKRFCDLPEDSLVITPSGMTV
                     FAPSVFSEQNKPLSIRPADLPQLSPRTASIISRSRFGDVVAAYLRGLKRGLDLSAVRV
                     PDLYLDHVNLTHRADEPGHRTDGQTLIASEAEESPFNRWYEAEMVKLCKEGMNLDRKF
                     GWLISWERGKSSVVQNEPHGRIYVSVVPRHAPAICRYLLALRKRRSGATPFEFKLALV
                     KEELERADPMVIYFSHKHQKTLLGYMNSMYDQIQARMQATRCSEEACWLKETNRIPPL
                     TLPLRHSRTGVVMRGLSFGESPINRGQSFGTQRKAAWEQSARWIALISHYADQIGLEI
                     DVENALITLGMHLHNVDPEYPAFNLRERDGRSGTDVFADLLKQCEEPTEFFP"
     gene            2830..3213
                     /locus_tag="VJC18_06730"
     CDS             2830..3213
                     /locus_tag="VJC18_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45114.1"
                     /translation="MSNATVTKWFKQADRSDFVNIETKSLFGLARGLGVDVSIFLSER
                     RRLQPLQTSFLWDNLFPNMETFVKAVVQKRLPALARLVEVLGLRNASLVAGDVIYKKF
                     SDYKKFIKPVRRQQMEVLCQILYQP"
BASE COUNT          859 a          829 c          780 g          904 t
ORIGIN      
        1 ctttaatctg ctgtgcaatc ttttcatttt ttgcaaacac caaaagcccc gaggtgtcgc
       61 gatcaagacg atgaacaatc gacaccaggc ggcgcttggg cttgcccaaa ctcatgtact
      121 ggcaaagcgc ccccacaaca gtgttgggct cacggtgatc ggtgggcaca gtcaggtacc
      181 ccgctttttt atcgacaacc aaaatccatt cgtcctcaaa aatcaggtca aagatcggcg
      241 accgatattt tttgggaagt tctttgtagt ttcggttttt atcgtaatga acagagacct
      301 cgtccgacgc ccgcaccctt tctcccgcgt tgtcacacac aactccgttt aacaaaacac
      361 aaccgtgatc aaaaagaccc ctgacttgcg tgcgcgaacg ctccgtgagt ttttgcacca
      421 caagatcaat gcgtcccgtt tcgttttctt tgattaaatg ttttaccgaa gtcataaata
      481 agtcatggag tacggtcact ctttgattat aaaattttgc gagacgacat cgattcgatt
      541 ctcgcgagac aaagggtcgt tcaagggaca ggtcacacgg taaatcccgg gctttgccgg
      601 ggcagatttt tccccatgcc acacacacca tgatgtcgtt tgtgtccaac tgaattcata
      661 tgtttttttg gggtcaatgt aggcttcaga catgttgcat tgcacatcgt catcacagga
      721 acaggaaaca ctgggaacgg ttgtccattg ctggttcaaa aattgttcca aggggcatgg
      781 cggatttgag tacgagtcga cacgcacact tttcccggaa tgattggtga cactgattct
      841 caccgcctca cccttggcgt aagcagtttt gtcggtggtc accaaaaaaa ggttgggggt
      901 gagctgtgtt ggcaatggtg gcgtttgtgc caaaaccgtt tgtttcacaa aaaaacaaat
      961 cactaaggat acaaataaaa tcggtgatat atttttcatt gtgcctcctg aacaaccaga
     1021 ccagccaata acaacgctgt gttaaacaat gtctcgaact tacgtcgttg agcataaatc
     1081 ggttttgcat ttaagtcgag agcactgatt ggtgccctta ggggaaaaat tcggttggtt
     1141 cttcacactg ttttaacagg tcggcaaaaa catccgtgcc cgaccgccca tctcgttcac
     1201 gcagattaaa agcagggtat tcaggatcca cattgtgcag gtgcatgccc aaagtgatga
     1261 gggcattttc aacatcaatt tccagaccaa tctggtctgc atagtgactg atgagcgcaa
     1321 tccagcgggc agactgttcc catgccgctt ttctttgtgt tccaaaagat tgccctctgt
     1381 taatggggga ctcaccaaaa gacaaccctc gcatgacaac acctgtgcgg ctatgccgca
     1441 acggcaaagt gagcggtggg atgcggtttg tttctttcag ccaacatgcc tcctcggaac
     1501 accgtgtggc ctgcatgcgc gcctgtatct gatcatacat gctgttcata taacccaaga
     1561 gagtcttctg gtgcttgtgc gaaaaataaa tcaccatggg atcagccctt tccaactcct
     1621 ctttaaccag ggccaatttg aattcaaacg gcgtggcccc cgaccttctt tttctgagcg
     1681 ccaacaagta gcgacaaata gctggagcgt gtctgggcac taccgataca tagatcctgc
     1741 catgaggttc gttttggaca acactgctct tgcccctttc ccaactgatc aaccagccaa
     1801 actttctgtc tagattcatg ccttccttgc acaactttac catttcagct tcgtaccatc
     1861 tgttgaaggg gctttcttct gcctcggagg ctatcagggt ttgaccgtct gttctgtggc
     1921 ccggttcgtc ggcacgatgc gtgagattga catgatcgag gtacagatca ggcaccctaa
     1981 cagcactaag atccagccct cttttgagac ctcgtaaata cgctgccaca acatcaccaa
     2041 accgactgcg gctgatgatt gatgctgttc tgggggaaag ctgtggcagg tctgctggcc
     2101 ttatcgataa aggtttgttt tgttcagaaa aaacagaggg ggcaaacaca gtcattccag
     2161 aaggcgtgat gacaagcgag tcttctggga gatcacaaaa tctttttatg gtgatcctga
     2221 caccttccct ttgtctgagc tcagaaacga caggcattcc tttcatagct ttgcctgtga
     2281 agaccgtgac atttccggag ggagcgatga tggcgcaggg gatgttgggt ctgcggggcg
     2341 tcagcagaga taccccgcct atatgctcaa tgtctacatg ggcctgtgga tcatccattt
     2401 ggccaggccg ccctttgaca tgctcaaaat ccccaagcct catgagcagt ggcaacaggg
     2461 gttgtcctgc aagatctcgc acaggtggct caatcccctt gggcatgaga gacgggcttg
     2521 ccatttcaaa accagaggct ttggccacaa cagtttgtcc catcacttct ggagacaatg
     2581 ccagctgtgt ggcaaaggga gatagtctgg ggatatctgg tgccatgcag atttcatcgt
     2641 caggtgttgt caaaagttgc ctgtaaacgg actccggact cccattcctg accttttctg
     2701 gttgattttt agcatcaaat cgtttactat agtaaataga ttttgttaat aaatatgtat
     2761 tttcaaataa ttaaaaacat tgccgcaaac agatctctac aaaaaacaga cctagcacgt
     2821 ttggcggcag tgtctaacgc cacggttaca aagtggttca aacaagctga caggtcagat
     2881 tttgtaaaca tcgaaaccaa aagcttgttt ggtcttgcaa gaggacttgg tgttgatgtg
     2941 agcatttttt tgagcgaacg acgacgactc caacccctcc aaaccagttt tttgtgggac
     3001 aaccttttcc ccaacatgga gacctttgtt aaggccgttg tccaaaaacg cttgcccgca
     3061 ctggcaaggt tggtagaagt gctcggactt cgcaatgctt cccttgtggc aggtgatgtg
     3121 atttacaaaa aattttcaga ttataaaaaa ttcatcaaac ccgtccgtcg tcagcaaatg
     3181 gaggtcttgt gccaaatact ttatcaaccc taactccttt tccacacatc gattcaaccg
     3241 tcacaacaat ggctcaacga gaagccttgc aaacaatctt tcaaggcctg gattttggtg
     3301 tgtggcttgt gggtggaaca gctttggcag gttactacgc aagacacaga gtttcttacg
     3361 atcttgatct ct
//
LOCUS       DATDUN010000481         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_561426, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000481 DATDUN010000000
VERSION     DATDUN010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561426"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VJC18_06735"
     CDS             complement(<1..778)
                     /locus_tag="VJC18_06735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HLD45115.1"
                     /translation="MKEVVIVSAVRSPMGRAHKGNFAYTRADDLAADVIRACLTKVPQ
                     VKPAEIENFFLGCAMPEGEQGMNLARNVVFLAGLPLSLPAGTLNNFCSSSLVTIMLGA
                     QGIMAGQGDIYLSGGVESMSHVPMGGYNPSLNPKLMQDGAPQAYISMGITAENVAVKY
                     NVTRQEQDAFALSSHQKAVRAQKEGLFTKEIVSVTSYAQDGKTAQMVIDQGPREDSSL
                     EKLATLKPVFKEGGTVTAGNSSPLTDGASATLLMSADKAKE"
     gene            complement(1035..1634)
                     /locus_tag="VJC18_06740"
     CDS             complement(1035..1634)
                     /locus_tag="VJC18_06740"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HLD45116.1"
                     /translation="MTRIYIDFDDVICRTAEIILELANQKFGIDCAFDDITDFNLQTS
                     FNLSDQQFKNLIDLIHEPEVLGDMRPVDGAISAMTELKKRGCELAIMTGRPPSTRASS
                     LAWLKHYGVPYDSIAFVDKYARFLYNEHDEGGVTLDELKQMSFDIAIEDSASMACFLA
                     VEMNVPVFLHDRPWNRSLAPHPKIQRCRDWMEIMSEISI"
     gene            complement(1644..>2444)
                     /locus_tag="VJC18_06745"
     CDS             complement(1644..>2444)
                     /locus_tag="VJC18_06745"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HLD45117.1"
                     /translation="SLGFLFSLIEKMSVRHQPVHVTVHAFEQNKKILADGVSLLRAFL
                     EAVSAKSMGLTVEVVPYAIDLARGRLPRVLSDVILVGNLLNELGRRDAQQLVLARMLD
                     SLCQKKFLLLMLEPAHKKISRDLQSLRDYLIQRFSLNVVGPCLHSLACPLNQVNKRDW
                     CHQRLIWDVPELIRDFDRLTKLKKVYLQYSYLLLRPDRVAGERSADTFLAITDVMLVG
                     RQKELVACSRGGLYRFVLPAATSQPRFSQLRRGDTFSPMPHVIMHKVV"
BASE COUNT          668 a          655 c          573 g          548 t
ORIGIN      
        1 attcttttgc cttatcggcc gacatgagga gcgttgccga agcgccgtca gtgagaggac
       61 tggaattgcc agccgtcacc gtaccacctt ctttaaagac gggcttgagt gtggccaatt
      121 tttcgagcga agaatcttca cgtggcccct gatcaatcac catttgtgca gtcttgccat
      181 cttgggcata ggaggtgacc gaaacaatct ccttcgtgaa caagccttcc ttctgtgccc
      241 gcacggcctt ttgatgcgaa gacagcgcaa aggcatcctg ctcttgcctg gtcacattgt
      301 atttgaccgc cacgttctcc gccgtaatac ccatggagat atacgcctgc ggagccccgt
      361 cctgcatgag tttggggttg aggctcgggt tatagccgcc catgggaacg tgagacatgg
      421 actccacgcc accagaaaga tagatatcgc cctgacctgc catgatcccc tgcgcaccca
      481 acatgatcgt gacgagtgac gaggagcaga aattattcaa cgtccctgcc ggtaacgaga
      541 ggggaaggcc cgccagaaaa accacattgc gcgcgaggtt catgccctgc tcaccttcgg
      601 gcatggcaca acccagaaag aagttttcga tttctgccgg cttcacctga ggcaccttgg
      661 tcaaacaggc cctgatcaca tctgcggcca gatcatcggc gcgcgtgtaa gcaaaatttc
      721 ctttatgtgc cctgcccatg ggtgagcgga ctgctgaaac gatgacgact tctttcataa
      781 caacctttca tttttatggt aacgtaaacg gaatcgtacg accagaccgt aggtgcgggg
      841 ctcgacccgc ccaacatcgc caacaatccc gtacaatacc aacagcattt tcgtcagtga
      901 aaaaaacaac tcacaaaaca aaaacaccaa ttagtaaaat caaacaataa aataatattt
      961 acatgctgtt agacacattc actgcatgaa cgctcattca ttttgtcgga gcatagtgat
     1021 tttttattga agaatcaaat agaaatttct gacatgattt ccatccaatc ccgacacctt
     1081 tgaatcttgg gatgaggggc cagggaacga ttccagggcc gatcgtgcaa gaaaacagga
     1141 acattcattt caacggccaa aaagcaggcc atgctggctg aatcctcaat ggcaatatca
     1201 aatgacattt gtttgagctc atccaacgtg accccgccct cgtcatgttc gttatacaaa
     1261 aagcgtgcgt acttgtcgac aaacgcgatg ctgtcatacg ggaccccgta atgcttgagc
     1321 caagccaggg aactggcacg ggtcgaggga ggacggcctg tcatgatagc caactcacag
     1381 cccctcttct tcaattcagt catcgctgat atggcgccat caacaggcct catatcaccc
     1441 agaacctccg gctcgtgaat aagatcaatg aggttcttga attgttgatc ggacaaatta
     1501 aaggatgtct gcagattaaa atcggtaatg tcatcgaagg cgcaatcaat accaaatttc
     1561 tgattcgcca gttcaagaat gatctcggcc gttctgcaga tcacgtcatc aaaatcaatg
     1621 tagatacggg tcatagaaag ccctcacacc accttgtgca tgatcacgtg gggcatgggc
     1681 gaaaacgtgt cgccgcgcct gagttgagag aaccggggct ggctggtggc cgccggcagg
     1741 acaaaccggt acagcccccc acgagaacag gccaccagct ctttttgtcg tcccaccagc
     1801 atgacatcgg taatggccag gaaggtgtct gcagaacgct cacctgccac gcgatcagga
     1861 cgcagtaata aataactgta ttgcaaataa acctttttta gtttggtcaa ccggtcgaaa
     1921 tccctgatca gctcgggaac gtcccaaatc aacctttggt gacaccaatc gcgtttattg
     1981 acctgattga gcggacacgc cagactgtgc agacacggcc ccacaacatt cagagaaaag
     2041 cgctgaatca ggtagtccct gagactttgc agatcgcgac tgattttctt gtgggcaggt
     2101 tccaacatga gcaagagaaa ttttttttga cacaaggaat ccagcattct ggccagcacc
     2161 agttgctgcg catcgcgtcg ccccaactca ttgagtaaat tgccaacaag aatcacatca
     2221 ctcaaaaccc ggggaagacg ccccctggcg agatcaatgg cgtaaggaac gacctccaca
     2281 gtcaacccca ttgatttggc agaaacagcc tccaaaaagg cgcgcaaaag agagactccg
     2341 tcggcgagta tttttttatt ttgctcaaac gcgtggacgg taacgtgtac aggctgatgc
     2401 cgaacactca ttttttcgat caacgaaaat aaaaagccca gact
//
LOCUS       DATDUN010000482         1690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_313116, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000482 DATDUN010000000
VERSION     DATDUN010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313116"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VJC18_06750"
     CDS             <1..799
                     /locus_tag="VJC18_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLD45118.1"
                     /translation="TNLGQYYYQYAWNDFCDWYIELCKVRLASEDQPQRRTALHNLLV
                     AFSKTLILLHPLVPHVTEELWSQMLPLGDRFSLWGDQRPQSKILMRETFMPQQALMPV
                     EQEILDRFGCFELFVSQLRTLRKTYQIKESQKLSACALPLDDTATRMLETSRAAICRL
                     AHLSELNVIADRTTKSARSVGIVEKGFEIFLDVSQYIDVVAEKARLQKEIDKLSVHVT
                     SLNKRLQNRSFVENAAPEVVAEQRETLSVNQDKIKKLEQLLSEMVGW"
     gene            complement(944..>1690)
                     /locus_tag="VJC18_06755"
     CDS             complement(944..>1690)
                     /locus_tag="VJC18_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLD45119.1"
                     /translation="PKLEKKYLWQVIGAGLCGMALPWIFLFWAEKTVAPALASLINST
                     TTLYVVLLTPLFNPKDKITSSQWGGVLVGIVGIAIIFWPDISFTDPNKQLLSMLALLA
                     TTSLYAVANQWSRRFSHIVDNQNNAYYQMLASAVVMLMMSLLFDPAQILPTFDIKALM
                     AVLYLGIFSTGIAILLNYWIIKNVGGVQSAAIVFLIPLVSITLDLVIIHKSLEPNQYV
                     GGAVILLALVLLNKKGGSWIVDRGSKKRIA"
BASE COUNT          469 a          403 c          444 g          374 t
ORIGIN      
        1 cacaaatctg ggacagtatt attatcagta cgcctggaat gacttttgtg actggtatat
       61 tgagttgtgt aaagtccgat tagcctcaga ggatcagccg caaagaagaa cagccttgca
      121 caatctgttg gttgcctttt ccaaaacgct cattcttttg cacccgctgg ttccgcatgt
      181 gaccgaggaa ttgtggtcac aaatgcttcc tttgggagac cggttttcgt tgtggggtga
      241 tcaaaggccg caaagcaaaa tcctcatgcg cgaaaccttc atgccccaac aggcactcat
      301 gcctgtcgag caggagattc tggatcgttt tggctgtttt gaactgtttg tttcgcagtt
      361 gcgaacgttg cgcaaaacat atcagatcaa ggaatcacaa aaactgtcag cctgtgcatt
      421 gcctctcgat gacacggcga ctcggatgct cgaaacatcg cgtgcggcca tctgccgtct
      481 ggcacacctg agcgagctga acgtgattgc cgatcggaca accaaatcgg cacgaagcgt
      541 cgggattgtg gagaagggtt ttgaaatctt tttggatgtg tcgcagtaca ttgatgtggt
      601 ggccgaaaag gcacgtctgc aaaaagagat cgacaaactc tctgtccatg tgacgtcact
      661 caacaaacgc ctgcagaaca ggagttttgt tgagaacgcg gcccctgagg ttgtggccga
      721 acaaagagaa accctgagtg tgaatcaaga caaaatcaaa aaactggaac agctcttgag
      781 tgagatggtg gggtggtagg ggggcaaaag aacgaaaaca cgaaaacacg aaaacacgag
      841 ggacgcttgc ccaccgaagc tcagagagca taggtgggag ggacgaaatc agctcaaagc
      901 tgaaagctga aagggaagaa ggaaatgcaa agagcgatcc ccgctacgca atacgctttt
      961 tcgatccacg atccacgatc cacgatccac ctttcttatt gagcaatact aacgccaata
     1021 aaatcaccgc gccgccaacg tactgattgg gttcaaggct tttgtgaatg atcaccagat
     1081 ccagagtgat cgagacgagg gggatcaaaa acacaatcgc cgcgctttgc acacccccca
     1141 cgtttttgat gatccaataa tttagcaaaa tggcaatccc ggtgctgaag atacccaaat
     1201 acaaaacagc catgagtgcc ttgatgtcaa aggtcggcag gatctgtgcg ggatcaaaca
     1261 agaggctcat catcagcatg accacggctg aggccaacat ttgatagtag gcattgttct
     1321 ggttgtccac gatgtggctg aaccggcgtg accattgatt ggccaccgcg tacagggatg
     1381 ttgtggccag cagggccaac atgctcaaaa gttgtttgtt gggatctgta aaagaaatgt
     1441 ccggccagaa aatgatggcg atgcccacaa tgccaacgag gacgcccccc cattgcgatg
     1501 acgtgatttt gtccttggga ttaaagagtg gtgtcaataa caccacgtac aacgttgtgg
     1561 tgctgttgat gagggaggcc agtgccgggg ccacggtctt ttcggcccag aacaaaaaaa
     1621 tccacggcaa cgccatgcca cacagccccg cgccaatgac ctgccacagg tatttttttt
     1681 cgagtttagg
//
LOCUS       DATDUN010000483         1882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_396320, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000483 DATDUN010000000
VERSION     DATDUN010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396320"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VJC18_06760"
     CDS             complement(<1..699)
                     /locus_tag="VJC18_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD45120.1"
                     /translation="MKDKITDKHSDEFLNENVSKTSIALLLFSTSESLIKTLSARITQ
                     DNEFDLEIGTLSAKTPDKALSLLSCHKIDIIIVDLTTAEDIGLALIKDSRQHCFEQPI
                     IVISPPNCLELIKKAFTYGAQEYFFDEGPCSGSHLTRIIKLTLERRELFNQMQERTRE
                     QFHEVINRCVENMIILNADGVVLYLNDSARTLLTDDENSFVNKVLDFSVFHADQGDGA
                     KPTLLSLKTIALLNQ"
     gene            954..>1882
                     /locus_tag="VJC18_06765"
     CDS             954..>1882
                     /locus_tag="VJC18_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45121.1"
                     /translation="MTTRRTAQLFLIIFSFVFCQCGLQQPFDSLSSTTIDQGSVGYDI
                     PVGSAIEKLSISNGGVTIDFENTDSEGQNDAVLIIYANDISANGTFFKLHPKNLEGTS
                     YLTKESSEVAKQSFESGELTFDDYLREFEESIDEDAELPELDETAQLQYLSKGSSRRF
                     NVINTFSASSYETIEATLRYENEFVKMYVDQRDDDSFSDDELATLAENFSELIAVECE
                     LFGDASDVNDDGKIAALFSRVVNKIGASQGGIVTGYFYAPDLMSKKKYPSSNEMEIIH
                     SLVPDPEGAFGVPVSKSFALNSIIPGVLPHELQ"
BASE COUNT          490 a          405 c          473 g          514 t
ORIGIN      
        1 ttggttgagc agggcgattg tttttaaact cagcagggtg ggctttgcgc cgtcgccctg
       61 atcggcatga aacacagaaa aatcgagaac cttatttacg aaggaatttt catcgtctgt
      121 cagcaaggtg cgagctgagt cgttcaggta cagcaccacg ccatcagcgt tgaggataat
      181 catgttttcg acacaacgat tgatgacttc atgaaattgc tcgcgtgtgc gctcctgcat
      241 ttgattgaac agctcgcgtc gctccagagt gagtttgatg atcctggtca agtgcgagcc
      301 agaacaggga ccctcatcaa aaaaatactc ttgtgccccg taagtgaagg ccttcttgat
      361 cagttccaga cagtttggcg gcgaaatcac gatgatgggt tgctcaaaac agtgttgtct
      421 gctgtctttg atgagagcaa ggccaatatc ttctgcggtg gtgagatcga caatgatgat
      481 gtcgatcttg tgacaggaaa gtaaggataa tgccttatcg ggtgttttgg cggataaggt
      541 cccaatctca agatcaaact cattgtcttg tgtgatgcgc gcggaaagtg ttttaatgag
      601 ggactccgag gtcgaaaaca gcaggagcgc aatgctggtc ttcgagacgt tctcgttcaa
      661 aaattcatct gagtgcttgt ctgtgatctt gtctttcatt gggatgtggg accatgacat
      721 tttctcaaat tgaagacaaa tgctaaatgt atgacgggtg ggctgttctc aatctgataa
      781 tttttatcaa tttatcagga acattttgac cgtcttttga cgctctgcgt ccaaaaccgg
      841 aagggtaaac agggtgatct ctttggaata attagaaata aagtttgttc tcttggtggc
      901 acaggtcttg ctttgagggt tggtaggaac tcaattcaaa gcggggacga gttatgacga
      961 ccaggagaac cgcacaatta tttttgatta ttttcagttt cgttttttgt caatgtggtt
     1021 tgcaacagcc ttttgattcc ctttccagca caacgattga ccagggtagt gtgggctatg
     1081 atatccctgt gggcagtgcg attgaaaagc tctccatcag caatggcggc gtgaccatcg
     1141 attttgaaaa tacagactca gagggccaga acgacgctgt tctcatcatc tacgccaatg
     1201 acatttctgc caacgggacc ttctttaagt tgcatccaaa aaatttggaa ggcacgtctt
     1261 acctcacaaa ggaatccagc gaagtcgcga agcagtcatt cgaatccggg gaactgacat
     1321 ttgacgatta cttacgagag ttcgaggaat cgatcgacga ggacgcagag cttccagaat
     1381 tagacgaaac ggcgcaactc caatatcttt ccaagggtag ttcgcgacga ttcaatgtga
     1441 tcaacacctt ttcggccagt agttatgaaa ccatcgaggc cacccttcgc tacgaaaacg
     1501 aatttgtaaa aatgtatgtc gatcaacggg acgatgacag cttttcggat gacgagctcg
     1561 ccaccttggc cgagaatttt tctgaactca ttgctgttga atgtgaatta tttggtgacg
     1621 catccgacgt caatgatgac ggtaaaatcg ccgcattgtt cagccgcgta gtgaataaga
     1681 tcggggcgag ccaaggcggt attgtgactg gttatttcta tgcccccgac ctcatgagca
     1741 agaaaaaata cccctccagc aacgaaatgg agattattca ttcgctggtg ccggatcccg
     1801 agggtgcctt tggggtgccg gtttccaaat cgtttgcctt aaacagcatt atccccggtg
     1861 ttctgccaca tgaattgcaa ca
//
LOCUS       DATDUN010000484         1777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_229307, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000484 DATDUN010000000
VERSION     DATDUN010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229307"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VJC18_06770"
     CDS             complement(<1..990)
                     /locus_tag="VJC18_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45122.1"
                     /translation="MFINKKIMIAGFLAFVLIAGINCGGSSTPTDEGTTGTVGNASGI
                     LTAPDGSPLAGATVALLDDSGAANLIKTATTNKFGHKIRLPRLTDDEGDECDDVSASV
                     TAGDIVASDCTDAEGRYILEGEIPCGEAVTLYAKLGSFELNVTLTITCGDDDDNGEYD
                     DADLTGIAFDDDCIDEEASDDTTEGGEGEGEGEGEGEGEGEGEGEPCLEKLTPDYDVD
                     AATCDFESANMAVVTGLWDRIEDVLAKLGFGSVDEYGMFDNTQPYGFTMIDGIDGSTD
                     YPDAWDFFSDLDEMNAYDIIFINCYDRNDLASDSTVLANLQSYVENGGKLYVTD"
     gene            complement(1262..>1777)
                     /locus_tag="VJC18_06775"
     CDS             complement(1262..>1777)
                     /locus_tag="VJC18_06775"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HLD45123.1"
                     /translation="VGRGSSFRPFVPSACPEPVEGSLRSSSLVFSCFRVFVSSCFLPL
                     LSALLITILDIIVDPVSHMGKRWFLGDIYYYPNPGWYFDITMSNFLGWFLVSFIISVI
                     GIHVLKFDRKAVVSKTNSILSLGLYFGIFGFGLALAVYLREWTLVLADLGWLVVVCLI
                     VFGKCRSASVL"
BASE COUNT          505 a          433 c          448 g          391 t
ORIGIN      
        1 gtcagtcaca tagagcttgc cgccattttc aacatacgat tgcagatttg ccagtacggt
       61 tgagtcactc gccagatcgt tacgatcata gcagttgatg aaaatgatgt cgtaggcgtt
      121 catctcgtcg aggtcgctga agaagtccca ggcatctggg taatcggtgg atccatcaat
      181 gccatcgatc atggtgaagc cgtagggctg ggtattgtca aacatgccat attcgtcgac
      241 actgccaaag ccaagtttgg caagaacgtc ttcaatgcgg tcccaaagac cagtcacaac
      301 tgccatgttg gctgattcga aatcacatgt tgccgcgtcg acgtcgtaat ccggtgtgag
      361 tttttccaga caaggttcgc cttcgccttc accttcacct tcaccttcac cttcaccttc
      421 gccttcgcct tcgccaccct cagttgtgtc atctgaggcc tcttcgtcga tgcagtcgtc
      481 gtcaaaagca atgcccgtga ggtcggcatc gtcgtattcg ccattgtcgt catcatcacc
      541 gcaggtgatg gtgagcgtga cgttgagttc aaaactgccg agcttggcgt aaagggtgac
      601 cgcttcgccg caagggatct caccctcgag aatgtaacgg ccttcggcgt ccgtgcaatc
      661 cgaggccaca atatcgcccg cggtgacaga ggcagagaca tcatcgcact catctccttc
      721 atcatcggtg agtctgggga gtctgatttt gtgaccaaac ttgtttgtgg ttgcggtctt
      781 gatcagattg gccgcgccag aatcatccaa cagggcgacc gtggcacctg ccagaggtga
      841 accatcgggg gccgtgagga taccagaggc attgcctaca gtacccgtag ttccttcatc
      901 ggttggcgtt gaagacccac cgcaattgat acccgcgatg agtacgaacg ccagaaaacc
      961 ggcgatcatg atttttttgt tgatgaacat gaaacctcca tgatggatac aagcgtgtgg
     1021 gcctgtatcg gtgttaatgt gtagaaaacg ctggcacatt catatacgat gtggtgggat
     1081 tgtcaaataa gtaatgggga aaagctcaaa gctgaaagct caaaggtgaa aggtgaaagg
     1141 aatatgaatg tcgaataatg tcatctctgt gaaagcaata tattcatggt tgtcattcct
     1201 gcgaaggcag gaatccagaa ttaatgtaaa tatggattcc agcattcgct ggaatgacaa
     1261 atcacaaaac actagcactc ctgcatttcc caaaaacaat caaacaaacg acgacgagcc
     1321 agcccaaatc cgccaggacc aatgtccact cacgtaaata gacggccaag gccagaccaa
     1381 aaccaaaaat gccaaaatag agtcccaatg aaagaatgga atttgttttg gaaacgacgg
     1441 cctttcgatc gaacttgagc acgtgaatgc caatcacact gatgatgaaa ctcaccaaaa
     1501 accaacccag aaaattactc atggtgatgt cgaaatacca gccaggattt ggatagtaat
     1561 aaatgtcgcc gaggaaccat cttttcccca tatgagatac tggatcgacg atgatgtcga
     1621 ggatggtgat gagcagggcg gagaggaggg ggagaaaaca cgaagacacg aaaacacgaa
     1681 aacacgaaaa cacgagtgaa gaagaacgaa gggacccttc gacaggctca gggcaggctg
     1741 aagggacgaa aggacgaaag gacgatccac gacccac
//
LOCUS       DATDUN010000485         1360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_273535, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000485 DATDUN010000000
VERSION     DATDUN010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273535"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1360)
                     /locus_tag="VJC18_06780"
     CDS             complement(<1..>1360)
                     /locus_tag="VJC18_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45124.1"
                     /translation="QLNADKEASLQTARDLRQTLCRQLAEDSNLPEEGWLLGLLTEEH
                     RLAQKRIPQRLSIFKNLALTWPQTFDEQMHYLHTNREVPRSRWLTFFLKDGKEYVFGT
                     PLDYEQIILGEEEGILRELMRFTSKKKWVLERIKKMPDSMTKLGLLEQYWHSYASLLL
                     AGERESVITLWHGLIIFLAGKLERWRHIEDTSLRAYYFQRSTPQTPVPCQHSHFYHKN
                     ILGLARACLAFEAHRVFGCFLLKGLTCEEGLHRSLYYDRSAKERLQYLSDQTVYRAYA
                     SYQGRVAALLLLADEETDQARHYLDLADAVLATYSVIPQTERALLGGHDAYLRGLVAA
                     RWFALGETDKAIKGLDEAIQRIDQINNDLQSEDEPPVIPERATLLHPDLFELTTCPRI
                     HGLQIPHMVERGYLAQRSPSAERAELLEIRKELLILAPLERMSETGHIPTQTEVSEIE
                     GLL"
BASE COUNT          334 a          365 c          312 g          349 t
ORIGIN      
        1 agaagaccct caatttcact cacttcggtc tgcgtgggaa tatgcccggt ttctgacatt
       61 ctttccagag gtgccagaat cagcagttcc tttctgatct caagcaactc cgcccgctcc
      121 gcgctggggc ttctttgagc caaataccct ctttccacca tatggggaat ttgtagcccg
      181 tgaatccgag gacaagtcgt cagttcaaac aaatcaggat gcaacaaagt cgccctttca
      241 ggaatgaccg ggggttcgtc ctcactctgc aaatcgttat tgatctgatc tattctttga
      301 atggcctcat ccaatccctt gatggctttg tctgtttcac ccagagcaaa ccagcgggcc
      361 gccactagcc cacgcaaata agcatcatgg ccacccagca gggctctttc cgtctgcgga
      421 atgacagagt atgttgccag cacagcatct gccaagtcca gataatgacg ggcctggtcc
      481 gtctcttcat cggcaagcag taaaagtgcc gccacgcgac cttgatatga ggcataggcc
      541 cggtagaccg tctgatcaga caaatactgg agtcgttctt ttgccgatcg atcataatac
      601 agacttctgt gaaggccctc ttcacaggtc aaccctttga gcaagaaaca gccaaacaca
      661 cgatgagcct caaaggccag gcaggcccga gcgagaccca gaatattttt gtgataaaaa
      721 tggctatgct ggcagggaac aggggtctgc ggggttgacc tctgaaaata ataggccctt
      781 aatgaggtgt cttcgatgtg acgccagcgt tcaagcttcc ctgccaagaa aatgatcaac
      841 ccatgccaca gggtaatcac ggactcacgt tcgcccgcca acaggaggga tgcgtaggaa
      901 tgccaatatt gctcaagcaa acccagcttg gtcatggaat caggcatttt tttgattctc
      961 tcaagcaccc attttttttt gctggtgaag cgcatcaact cccgcagaat accttcttct
     1021 tctcccaaaa tgatctgttc atagtccaga ggggttccaa aaacatattc ctttccatcc
     1081 tttaaaaaga aagtcagcca gcgactgcgg ggaacttccc gatttgtgtg caaatagtgc
     1141 atctgttcgt caaaggtctg aggccaagtc aatgccagat ttttaaaaat actcaacctc
     1201 tgtggtattc ttttttgagc cagtctgtgt tcctcggtga gaagccccaa taaccatccc
     1261 tcttctggca agttactgtc ttccgcaagt tgtcgacaga gcgtttgcct caagtctctg
     1321 gccgtttgca gggacgcttc cttgtcggcg ttgagttgtt
//
LOCUS       DATDUN010000486         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_190918, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000486 DATDUN010000000
VERSION     DATDUN010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190918"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VJC18_06785"
     CDS             <1..227
                     /locus_tag="VJC18_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45125.1"
                     /translation="SPSLSPQGGIPFAGQKGGAVPSCCLLRNKISKRKAFFTHFYVVH
                     PLIVNLLTKQKKRATFSCPEAIQQHNHDVS"
     gene            283..1362
                     /locus_tag="VJC18_06790"
     CDS             283..1362
                     /locus_tag="VJC18_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45126.1"
                     /translation="MNCQPQNSNSSTSGAIPSPVTEPTSPTTGTETDTETSPETEPNT
                     ESEISGESGETGDDSSVPDVSDTDTEETTEEESSPGIPYGFWGLNGFHDSEGFLDVQT
                     RFGITVFQVASESPSWTVNTFLPLIRESGMKVTLRMTSAPDYSGDFNLEEWKTALSAW
                     IDSGVQEFIDDGTLIGHMILDDIYNFSGTDPTAEDLDEMARYSKEILPGLMTFVRQKA
                     TGMPVPVGGTYVYLDACVNQYSAYTGFPDGPIEEYVVEQAATARVLGLGIINGMNIAD
                     GGNGTSGQSGWSSGKFAMSAEEITTYGEALLGVPDLLMFLMWEYDGEEVWSDGILGSD
                     YFDQAERQEAIYGLGQIAAASGGGS"
     gene            1432..2064
                     /gene="rsmG"
                     /locus_tag="VJC18_06795"
     CDS             1432..2064
                     /gene="rsmG"
                     /locus_tag="VJC18_06795"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HLD45127.1"
                     /translation="MKIPPDNIESVLRERAHCNQISLSPGQIEAITAYHALTVKWHEK
                     INITTNTQPEAFVRENIVDPALACMALGFKNDACLRILDVGCGGGFGGIVFKLLCPEL
                     LVTLIDSDRKKISFCRQVMCALGLGGCEAHHTRVQDYRPGCLFDRVITRATFSQIEDC
                     QRACQHVLKESGHIVMFRSFSETEDAHQKHQRLLYTIKPEGIKRSLLMLP"
BASE COUNT          554 a          507 c          551 g          550 t
ORIGIN      
        1 ggagcccctc cttatcaccg caaggcggga tcccgtttgc gggacaaaaa ggaggggcgg
       61 tgccctcctg ttgcttgttg cgcaataaga tttcaaagag aaaagccttt tttacccact
      121 tttatgtcgt acacccgctc attgtgaacc tgttgacaaa acaaaaaaaa agagcaactt
      181 tcagctgtcc agaggcgata caacaacata accatgatgt gtcataaatt aatacaatcc
      241 ctttgcttgt tgctctttct tttttttatc agtgccgcgt ttttgaactg ccaaccccag
      301 aattcaaatt cttcaacatc gggcgccatt cctagtcctg taaccgagcc aacctctcca
      361 acaactggca ccgaaaccga tacggaaacc agccccgaaa cagaaccaaa tacagaaagc
      421 gagatcagcg gtgaaagcgg tgaaaccggt gatgattcaa gtgttccaga tgtctctgat
      481 acggatactg aagaaacaac agaggaagaa agctcaccag gtattcctta tggcttttgg
      541 ggcctgaatg gttttcatga ttcagaaggt tttttggatg ttcaaacacg ttttggcatc
      601 acggtttttc aggtggcctc tgaaagtccc agctggacag tcaatacttt tttgcctctc
      661 atcagagaat cgggaatgaa agtcacgctt cgcatgactt ctgctccaga ctattctgga
      721 gactttaatt tggaagagtg gaaaacagcg ctcagtgctt ggattgattc cggtgttcag
      781 gaatttattg atgatggcac gttaatcggt cacatgattt tagatgatat ttataatttt
      841 tctggaacag atcccacagc agaagatctg gatgagatgg cgcgctacag caaagaaatt
      901 ttgccggggc tcatgacgtt tgtgcgccaa aaagcaacag ggatgcccgt gcccgttggg
      961 ggcacctatg tttatctcga tgcgtgtgtg aaccagtatt cggcctatac cggatttcca
     1021 gatggtccca ttgaagagta tgttgttgaa caggccgcga ccgcacgggt cttgggtttg
     1081 ggaattatca acggaatgaa cattgccgat ggcggtaacg gcaccagtgg tcagtctggc
     1141 tggtcatcgg gaaaatttgc catgtcggct gaagaaatta ccacctacgg agaggccctt
     1201 ctgggggttc ccgatctttt gatgtttctc atgtgggaat acgatggcga ggaagtctgg
     1261 tccgatggga tccttggcag tgattatttt gatcaggccg aacgacaaga agcaatctat
     1321 ggactgggcc aaattgccgc cgcctctggc gggggcagtt gatgctttca acgaacgaaa
     1381 cttttttttg actttgacaa acaggccgtc ttgcgggcaa ggttctgcac gatgaagata
     1441 cctcctgaca acatcgaatc tgttttacgg gagagggccc attgcaacca gatcagttta
     1501 agtcccgggc aaatagaggc catcacggcc tatcacgccc tgaccgtaaa gtggcacgag
     1561 aagatcaaca tcaccacaaa cacgcaaccg gaggcctttg ttcgtgaaaa tatcgttgat
     1621 cctgccctgg cctgcatggc actgggattc aaaaatgatg cctgtttgag gattctcgat
     1681 gtgggttgtg gcggcgggtt tggcgggatt gttttcaaac ttttgtgtcc tgaactgctt
     1741 gtaacgttga ttgattccga tcgaaaaaaa atctcgtttt gcagacaagt gatgtgtgca
     1801 ttgggtcttg gcggctgtga ggcgcaccac acgcgggttc aagactaccg gccaggttgc
     1861 ttgtttgaca gggtcatcac gcgggccacg ttttctcaaa ttgaagactg ccagagggcc
     1921 tgtcagcacg ttctcaagga atcggggcac attgtcatgt tccgatcttt ttcggagaca
     1981 gaggacgcgc accagaagca ccaacggctt ctctatacaa tcaaacccga aggcatcaag
     2041 agatcgttgc tgatgctgcc ttaaccgact tcgacaagcc ccgcacccca acaaaaaccg
     2101 gcgccgaaac tggccaggac gagcctttga ttttttttga tgagaccggt agaacacagg
     2161 cc
//
LOCUS       DATDUN010000487         2848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_606489, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000487 DATDUN010000000
VERSION     DATDUN010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606489"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VJC18_06800"
     CDS             complement(<1..875)
                     /locus_tag="VJC18_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45128.1"
                     /translation="MSQDKSTEVPQETGSAPPKKVRCRVRRRVPFFQYRTFWFLLFVL
                     LGIVFTAMLNRAMNDREYQDKFANYLWEKSQIRLKFYDIKFNLFTGKVGGSELSVYAQ
                     KNRTLLSLSEFTLKYNPFYLWIGRFKITGVRAREFFLDTSQAVKAKSKNKKFDPKMIP
                     FFLKHVHLKQAAINKFDWLQPRGGHVLMDDIQLTSKFGSGLHTSPLQLRVNGLEYRGH
                     KQHAFVKRLALDGFFLFDFSQPLIFDQSRISAQVSLTDALFGFYRRPKPWLTDAVFAK
                     DLEPLIRQYYREIPD"
     gene            complement(1187..2605)
                     /locus_tag="VJC18_06805"
     CDS             complement(1187..2605)
                     /locus_tag="VJC18_06805"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HLD45129.1"
                     /translation="MRKNIFSLLLIVGLLFIACPSWAQESTSDVVPSFTDEELKAMVA
                     RIALYPDPLLAQVLPAATYPLEIVEAARLIRDKSDHDKIDKQDWDPSVKAVARYPSVL
                     KMMSDDLDWTEQLGQAVIGQQEDVMQAVQVMRAQAEKVGHLKTTKQQIVTRSDKVIEI
                     VPATPEVIYVPVYDPTVVYVLPVRHYYHPVIRFGVAFPIGSWLNIGFYWSSWGIYHCD
                     PYWWHGGWHHHYHHHYYSHHHYDHHYATNITHITNINNYYSKKTVKVASYKQYQKKDW
                     RHDNRHGTPYSGRKAYSQNTSRPNPDTFGRYTNNKSKTESTQNVRTSPQTTDRSKATT
                     RTTQKDNERNFEQAMNRVKQRDKTPAGQTTPSRESSAKKDQGRVAKAQERTHSYKPKI
                     NSRYKAPPTETSRVSTKSTKSTTSTRSSHESTASHSSSTKPRSSSFSSPSSSSSSYRS
                     RISSSHAPSLPKRYSSKSTHRK"
BASE COUNT          657 a          621 c          716 g          854 t
ORIGIN      
        1 tcatcgggaa tctctctgta gtattgcctg atgagaggtt ccagatcctt ggcaaacaca
       61 gcgtctgtca gccaaggctt gggtcggcgg taaaatccaa acaacgcatc agtcagactc
      121 acctgcgccg agattctgct ctgatcgaag atgagtggtt gcgaaaaatc aaaaagaaaa
      181 aaaccgtcca acgccagacg cttcacgaat gcgtgctgtt tatgtcctcg atattcgagc
      241 ccgttcaccc gtaattgcaa aggacttgtg tggagaccag atccgaattt tgaggtgagt
      301 tggatatcgt ccatgagcac atgaccaccc cggggctgga gccagtcgaa tttgttgatc
      361 gcggcttgtt tgaggtgcac gtgtttgaga aaaaacggga tcattttggg atcaaatttt
      421 ttgtttttgg atttcgcttt gacagcctgc gaagtatcca gaaagaactc ccgggcacga
      481 acccccgtga ttttgaaccg cccgatccag aggtaaaagg ggttgtattt gagcgtgaac
      541 tcgctcagcg acaacaaggt ccggtttttc tgggcataca ccgacagctc gcttcccccc
      601 actttaccgg tgaacaagtt gaatttgatg tcataaaatt tgagcctgat ctgtgatttt
      661 tcccagaggt aattcgcaaa tttatcctga tactcgcgat cattcatggc cctgttgagc
      721 atggcagtga aaacaatacc caacaggaca aataacaaaa accaaaatgt ccggtattga
      781 aaaaacggca cccgccttcg gacacgacac cgcacttttt ttgggggagc tgaacctgtt
      841 tcttggggga cctctgtgga cttgtcctga gacatgaaaa gtgaatatat ttatggggtt
      901 ttctatttta aatcaatggg ttttagggga gaatccgtgt tcaagtgttc aagtgttcag
      961 gtgttcaggt gctatcaggc gtcagacaaa agattcgtag gggcgagtcg cccaaaaaga
     1021 aaatttcttt tccacaatgc tgggccagtc acaatttctc catttctttc agccgtaaga
     1081 ctgatcccac tttacgggac attgtttcat tgtttcattg tttcattgat tcattgtttc
     1141 attgtttcat tgattcattg attcattgtt acattgattc attgattcat ttcctatgtg
     1201 tcgatttgct ggaataacgc tttggcaatg aaggtgcgtg tgaactggat attctggaac
     1261 gatatgagga cgaagatgac gatggcgaag aaaacgatga tgatctcggt ttggttgatg
     1321 aagagtgtga ggctgtgctc tcatgactgg atcgtgtgga tgttgtggat tttgtagact
     1381 tggttgacac gcgcgatgtt tctgtgggcg gggctttgta tcggctgttg attttaggtt
     1441 tgtaggaatg ggttctctct tgcgccttcg caactctgcc ttgatccttt ttggcagatg
     1501 actcccgact tggggttgtt tgacccgcag gggttttgtc tcgctgtttg accctgttca
     1561 tggcctgttc aaagttacgc tcattgtctt tctgggtggt tctcgttgtt gcctttgaac
     1621 gatccgtggt ctgagggctg gtccggacat tctgtgtgct ctctgttttg gatttgttat
     1681 tcgtgtatct gccaaatgta tcgggattgg gacgactggt gttttgcgaa taggccttcc
     1741 tgcccgaata cggtgtgccg tgccggttat cgtgccgcca gtcttttttc tgatattgtt
     1801 tgtacgacgc caccttgact gtttttttgc tgtaataatt gtttatgttg gtaatgtggg
     1861 tgatgttggt tgcgtaatgg tgatcataat gatggtggct gtagtaatga tggtggtaat
     1921 gatgatgcca ccctccgtgc caccaatagg gatcacaatg atagatgccc caggaagacc
     1981 agtaaaaacc gatgttgagc caggaaccga ttggaaacgc cacaccaaac ctgataaccg
     2041 ggtggtagta atgtctgacc ggtaaaacat agaccactgt gggatcgtag acaggaacgt
     2101 aaatgacctc tggggtcgcc gggacaatct ctatgacctt gtcactgcgg gtcacgatct
     2161 gttgttttgt ggtcttgaga tgccctacct tttcggcttg cgctctcatc acctgcacag
     2221 cctgcatcac atcctcttgt tggccaataa cggcttgccc cagttgttcg gtccaatcca
     2281 ggtcatcact catcatctta agtacactgg gataacgggc aaccgccttg acgctggggt
     2341 cccaatcctg tttatcgatt ttgtcatgat cactcttgtc tctgataagg cgtgcggcct
     2401 caacgatttc cagcgggtag gttgccgccg gcaggacttg tgccagcaag gggtctgggt
     2461 acagggctat gcgcgcaacc attgctttga gttcctcatc tgtaaagctg ggaaccacgt
     2521 cggaagtgct ctcctgagcc cagctgggac aggcgatgaa cagaaggcca acaatcaata
     2581 acaacgaaaa aatatttttt ctcatgtcgg ctgtaacgca agtcggcagg ggatggttct
     2641 atttcgttgt cagcacaaat cattccccgt accctgacgg attacttttt tctctttgtt
     2701 tttattgaca tttacacaaa acctctatta aggctccctt ttatgcgcat ttatgcgatt
     2761 tatagcaact ttttgcagtg aacaccgata atatgcttgt cacgtaacct ttttagttaa
     2821 ggagtacatt atgggcgata acgttaca
//
LOCUS       DATDUN010000488         1604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_64712, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000488 DATDUN010000000
VERSION     DATDUN010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64712"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            518..733
                     /locus_tag="VJC18_06810"
     CDS             518..733
                     /locus_tag="VJC18_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLD45130.1"
                     /translation="MTKIRENLANQKEYLSVADMAAEFGIHEMTFYRMIRDKKIPAFK
                     IGSQWRVRRQDLNKWLESHSSIPTDHA"
     gene            complement(839..>1604)
                     /locus_tag="VJC18_06815"
     CDS             complement(839..>1604)
                     /locus_tag="VJC18_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45131.1"
                     /translation="DSLYIIHIFNALCFSSIIFLTDGKQLILAEMYALSLVASFVINM
                     GSLIVYRYQKGKTEAREFITSRTITVCLFLLLLGCFIYLIINKPYGTMLWLIATIMAV
                     VVGLIYAQKHSPEIVQAGKGDSSMDIIMYVAGSEEKNVNIYFKRPFDLASNKLYDLSL
                     YVTFYSPRKDIPPKLAPNHFRLPAKRINLLHCIRALLDLINFELPEHNVTVHLGWPMS
                     SWIERMSVGVMIYQVMKLPKRYPKFNFKIEQFAAKK"
BASE COUNT          452 a          375 c          372 g          405 t
ORIGIN      
        1 gcgaatgtct tgaggtcctc aactcagagg tcgccgagtt gctggcgcaa agtattttaa
       61 ccaacaaaaa attcacaaaa attcgtgaac aactcaccag caacaaaatc aacccctacg
      121 ccgccgtcat cgaactcaaa aaactggttc gcattgcaaa ataataactc cttgcgccct
      181 acgccttgta cctaacgcct tttgttgccg tccccgataa tcattcaaat gcaaggcgtc
      241 cataaaattg caaccggagc gtatatggaa atacgtgagg attgcgattt tatggacaac
      301 acagcagatg gatgattatc ggggacggca ttatcaaaat gagtcattgt tgcctaatat
      361 actaggcaat tttggtcatc ctttgacagt tttttcaccc tacctcaacc aataaaaagg
      421 tctattctca ataatttcaa ggtgttattt ttttttggca aatggcacgc cctatgcatt
      481 gtacatgagt ataaaacgct taacacaggg gtacattatg actaaaattc gagaaaatct
      541 agcaaaccaa aaagagtacc tgtctgttgc cgacatggcg gcagaatttg ggattcatga
      601 gatgacgttt tatcgcatga ttcgggacaa gaagatcccg gccttcaaga tagggtcaca
      661 gtggcgcgtg cgccgtcagg accttaacaa gtggttggaa tcgcatagtt cgatcccgac
      721 ggatcatgct tgattccaaa ggcttacagc ttacagctga cagctgacag ccaataggca
      781 caaaccaatg gtcaccaatc ctgaccgtgg gctgttgcct gttggctttt tgtgtttctc
      841 atttcttcgc cgcaaattgt tcgatcttga aattgaattt gggatatcgt ttgggcagtt
      901 tcatcacctg ataaatcatc acccccacgg acatgcgttc aatccaactc gacatgggcc
      961 agcccaaatg cactgtcacg ttgtgctcgg gcaattcaaa gttaatcagg tctagcaaag
     1021 cgcgtatgca gtgcagaagg ttgatcctct tggcaggcag acgaaaatga ttgggggcca
     1081 acttgggcgg gatgtccttg cgtggtgaat aaaacgtcac atacagggag aggtcataca
     1141 atttgttact ggccagatcg aagggtcgtt taaaataaat attcacattt ttttcttcac
     1201 tgcccgccac atacatgatg atgtccatgg acgaatcgcc cttgcctgcc tgcacaatct
     1261 cgggggagtg tttttgcgcg tagatgagcc ccaccacgac agccatgatg gtggcgatca
     1321 gccacaacat ggtcccgtag ggtttgttga tgatcagata aatgaaacac cccagtagca
     1381 gcaggaagag gcagaccgtg atggtacgcg aggtgatgaa ctccctggcc tcagtcttgc
     1441 ccttttgata gcggtacaca atgagcgaac ccatgttgat cacaaaactc gccaccagac
     1501 tcaaggcgta catttcggcc agaatcagct gtttgccgtc agtgaggaaa atgatggaac
     1561 tgaaacaaag cgcgttgaaa atgtgaatga tgtacagcga gtcc
//
LOCUS       DATDUN010000489         1022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_107349, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000489 DATDUN010000000
VERSION     DATDUN010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107349"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VJC18_06820"
     CDS             complement(<1..961)
                     /locus_tag="VJC18_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLD45132.1"
                     /translation="MRRKKNTSPVKKYFESEPLPQRELVTLLKKTSLFSNVSPFFIRH
                     LAITSFREQHGAGSVIFKQGEPGNELYLVVRGEVIISRKEKSQPPARVATLYAGHCFG
                     EGALLSNLPRNATARAVTETELLVISKKEFDALCRKSSAFRRGIQGIFSERIQTENKT
                     EIESDVIAVVNQTRLSSHLLCLRIAQHLYDQYGEKSLLVEMVSNPTQSSAKPQEVTPG
                     VFAVKSSKRVSNLQGNIDKFKDVLHSHYIFCYGEAPLSENDFKKISGVLSRVLWLSDL
                     PGAVVPFHMSDALIVHQVMVRHPHALWPTAVPVTPGTLMFPADL"
BASE COUNT          261 a          258 c          241 g          262 t
ORIGIN      
        1 caagatcggc tggaaacatg agtgtccccg gggtaacagg aacagcggtg ggccacaagg
       61 catgcggatg acgtaccatg acttggtgta cgatgagggc atcggacatg tgaaagggaa
      121 caacggcccc tgggagatcg ctcagccaca acacacggct taagacccca gatatttttt
      181 taaaatcgtt ttcggataaa ggggcttcac cgtaacaaaa gatataatgc gagtggagaa
      241 catctttaaa cttgtcaata ttcccctgta gattggaaac acgtttcgaa ctttttaccg
      301 caaaaacacc cggcgtcact tcctgcggtt ttgcagagct ttgtgttgga ttcgaaacca
      361 tctccaccaa aagacttttt tctccgtact gatcgtacag gtgctgggca attcgcaaac
      421 acaataaatg tgacgacaac ctcgtttggt tgaccacagc aatcacatcc gattcgattt
      481 cggttttgtt ctccgtctgt atcctttccg aaaatatccc ctgaataccc cggcgaaagg
      541 cagacgactt acggcacagg gcatcgaact cttttttcga aatcaccaaa agctcggtct
      601 ctgtgaccgc tctggccgtg gcgtttcgcg gcaggttgga gaggagggca ccttctccaa
      661 aacaatgccc agcgtacaat gtggccacgc gggctggcgg ttggcttttt tcttttcggg
      721 agatgatcac ttcacctctc accaccagat acagttcatt cccgggctct ccctgtttaa
      781 aaatcacact gcccgcaccg tgttgctccc gaaacgaggt gatggccaaa tgacggatga
      841 aaaatggcga gacattcgaa aacaaggacg tcttcttgag cagggtcacc agctcccttt
      901 gtggcagggg ttcactttcg aaatattttt ttacgggcga tgtatttttt tttctgcgca
      961 tgtcatccaa aattgtaaac tctcacccca caaatgtaaa tcgctgattt tggacgtgga
     1021 tc
//
LOCUS       DATDUN010000490         1228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_274211, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000490 DATDUN010000000
VERSION     DATDUN010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274211"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VJC18_06825"
     CDS             <1..349
                     /locus_tag="VJC18_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HLD45133.1"
                     /translation="DHLGVFAYSDEDDTPAHTMPGHLSEELMTERKTELMELQRGISL
                     KKNKALVGKKIKVLFEGTSSESELLYQGRHAGQTPEIDGVVLINSGTAKVGTFCTVKV
                     TEAHEYDLVGKVV"
     gene            439..>1228
                     /locus_tag="VJC18_06830"
     CDS             439..>1228
                     /locus_tag="VJC18_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45134.1"
                     /translation="MKNKSFFLLSLVLLVGFFVVILSCGGSDPATDTTDDDDDAPTTE
                     DTSGEPYDMGDPTFTELYVSPSGSDSNSGTSIDEPLESLPAAWALIPTTLTTTGYRIN
                     MLPGDYPCGDDELHDCQNYFSDREGTYDAPIMIRSVDAEGEYEAGTATVRGGMDVNDV
                     SYLYVLGVDMIAGREEDGLPMNNSGNNIFHVANSDHILLRDATLTGPTGLTDTTSNVQ
                     EVVKANTSEAIYLENNDLSHSHQTVVDYFSTNGGHVINNHIHESG"
BASE COUNT          316 a          340 c          310 g          262 t
ORIGIN      
        1 tgatcacttg ggagtgttcg cctattccga cgaggacgac actcccgcac acacaatgcc
       61 aggccatctt tcggaagagc tgatgaccga acgcaagacc gaactcatgg aactgcagcg
      121 cggcatttcg ctcaaaaaga acaaggccct tgtgggcaaa aaaatcaaag tcctctttga
      181 ggggacatca agcgaatccg aactcctgta tcagggccgt catgctggtc agacaccaga
      241 gattgacgga gtcgtgctca tcaacagcgg cacagcaaag gtcggcacat tttgcacagt
      301 caaagtcacc gaggcacatg agtatgattt ggtggggaag gttgtttgaa tatcgaatat
      361 ccaatcacga acagggaatg tcgaagtgtg ttgtcatttc ccagttgacc ccatcataaa
      421 atcatctaaa taccatccat gaaaaacaaa tccttttttt tattgtcttt ggttttgttg
      481 gttggctttt ttgtcgtgat cctctcctgc ggcgggtctg atcctgccac ggacacgacc
      541 gatgatgacg acgatgcgcc aaccactgag gacacatctg gtgagcctta cgacatgggc
      601 gaccctacgt tcactgagct ttatgtctca ccgtcaggtt ccgacagcaa cagcggcacc
      661 agcatagacg aacccctgga gtccctgcct gcggcttggg ccctgatccc caccacactc
      721 acgaccaccg gttatcgcat caacatgctc ccgggcgatt acccctgtgg tgatgacgaa
      781 ctgcacgatt gccaaaacta cttcagcgac agggaaggca cctacgacgc acccatcatg
      841 atcaggtctg tcgatgctga aggagaatac gaggccggta cagccaccgt ccgtggtggc
      901 atggatgtga acgacgtgag ttacctgtat gtgctgggcg tggacatgat cgcgggacgc
      961 gaggaagacg gtttgcccat gaacaattcg ggcaacaata tttttcacgt tgccaacagc
     1021 gatcacatcc ttctgcggga tgccaccctc acagggccca ccggcctgac tgacaccacc
     1081 agcaacgtgc aagaggtggt caaggccaat accagtgagg ccatttatct ggaaaacaac
     1141 gacctgagcc actcgcacca aaccgtcgtg gattattttt ctacaaacgg tggccacgtg
     1201 atcaacaacc acattcacga gagcggcg
//
LOCUS       DATDUN010000491         1555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_480853, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000491 DATDUN010000000
VERSION     DATDUN010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480853"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VJC18_06835"
     CDS             <1..846
                     /locus_tag="VJC18_06835"
                     /inference="COORDINATES: protein motif:HMM:NF036972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HLD45135.1"
                     /translation="NGPFRNRTELLGVKGLTPKHFFLAGGFLIIPDSENLLDRTLIHP
                     EHYDLVNAMAGKLGKTLSELFAQPESFRELPIKEFEGPETSLESLRLIIGEFRAPAKD
                     PRADYEVLEPDPTIKSLDDLKPKMVLRGRVINLASFGAFVDIGVEQLALLHKSALSES
                     RDAPPPPTIKLGRVLNFEVLEVDKDKKRIQVGFPVFKRREPPPRRQEERPTRDNGRPA
                     YAKASTYAKASVDRSGQKCDERRGDNRFTPGKRSQGNLRPDRRPGPPKHQLGTLGDKF
                     KDLFK"
     gene            843..1427
                     /gene="folK"
                     /locus_tag="VJC18_06840"
     CDS             843..1427
                     /gene="folK"
                     /locus_tag="VJC18_06840"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HLD45136.1"
                     /translation="MMVTAYIGFGSNLGDRQAHLRATLDRLSACDHITLKRVSRLYQT
                     EPHKKNTDPQPWYLNCVLEIETSLTLHQLLSTLKLIEKELGRKTKSDWKPRPVDLDIL
                     LYGTIIYQDNGLRVPHSDLTQRRFVLQPLCDLCPTFLHPEYQMSLQELLNCCTDKLKV
                     VDWENKRNVFQISHCDPHPPFPHQIHLQKHQKPL"
BASE COUNT          422 a          461 c          343 g          329 t
ORIGIN      
        1 aacggtccct tcaggaaccg aaccgaactg ctcggcgtca aggggctcac gccaaaacat
       61 ttttttctgg ccggtggttt tttgatcatc cccgacagcg aaaacctcct cgaccgcacc
      121 ctgattcacc ccgagcatta cgacctggtc aatgccatgg cgggcaagct gggtaaaaca
      181 ctcagcgaac tttttgccca accagaatca ttccgcgaat tgcccatcaa ggaatttgag
      241 ggcccggaaa caagcctgga aagtttgcgc ctgatcattg gggaattcag agcccccgcc
      301 aaagaccctc gggcggacta tgaagttctg gagcccgacc ccaccatcaa atctctcgat
      361 gacctcaaac ccaagatggt gctacgtggg agggtgatca atttggcaag ttttggcgca
      421 ttcgtcgaca tcggcgtgga gcaactggcc ttgctacaca aatcagcctt aagcgaatcc
      481 cgcgacgccc cgccaccacc aacgatcaaa ctgggacggg tactcaattt tgaggtgcta
      541 gaagtcgata aagacaaaaa acgcattcag gtaggatttc ccgttttcaa acgacgagag
      601 cccccccccc gacgacaaga agaacgacca acgagagaca acggacgacc cgcctacgct
      661 aaagcttcca cctacgctaa agcttcggtg gacaggtcgg ggcagaaatg tgacgagaga
      721 cgaggtgaca acagattcac tcccggcaaa cggtctcaag gaaatctcag accagatcgt
      781 cgtcctggcc cacccaaaca ccagttggga actctgggtg acaaattcaa ggacctgttt
      841 aaatgatggt gactgcttac atcggcttcg gttccaatct gggcgatcgg caggcgcatt
      901 tgcgtgccac cctcgatcgg ctctctgcct gtgaccacat cacactcaag agagtgtctc
      961 gtttgtacca gaccgaacct cacaaaaaaa acactgaccc ccagccctgg tacctcaact
     1021 gtgtcctcga aatcgaaact tcactgaccc tgcaccaatt gttgtccacc cttaagctca
     1081 tcgagaaaga actgggccgc aaaaccaaat ccgactggaa accccgtccg gttgatctcg
     1141 atatcctgtt gtacggcacc atcatttatc aggacaatgg gcttcgcgtg cctcattcag
     1201 atttgacaca gcgacgattc gtgctccagc ccttgtgcga tctctgcccc accttcctcc
     1261 accctgaata tcaaatgagc ttgcaagaac tcctcaactg ttgcacagat aagttgaaag
     1321 ttgtcgattg ggaaaataaa cgaaatgttt ttcaaatttc tcattgtgat cctcaccctc
     1381 catttcctca tcaaattcat ttacaaaaac accaaaaacc gctttaaggt aaaccgcaac
     1441 gggcaacagg ggcgcacaca aatccatcag cccacgacct tgtgttcctg ccctgcctgc
     1501 gccacttttt tttacgaaac acaaggagta caaagacacg gcaagcttta ctgca
//
LOCUS       DATDUN010000492         1754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_439765, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000492 DATDUN010000000
VERSION     DATDUN010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439765"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1754)
                     /locus_tag="VJC18_06845"
     CDS             complement(<1..>1754)
                     /locus_tag="VJC18_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45137.1"
                     /translation="NAGSFSEPTKPLPVIKLSGVDDGTTTPSVSKGEYRGLKASGYKV
                     EQPEDRRSGTTHFFKPVIIARDGTRLILRFEDVESTKRSGRIHTWDMRNETVESGPFS
                     LISEAPEDRKKNANQFRANDELWVLPNCGDVLAYRYRGKKGSAFDGCELTVLLGADRV
                     SRTSALRKRIMGFVIDPAHRALFDAQFILTEPLPADEWRLSRDDLSITLAVRCFREQA
                     KYVLREYPVGTKLLPHPLGCADRRLLIMPGGDHIELEFTDDGRLLAHDHCHKINLVNG
                     KMEMPLTDWRGRKRRVMIERNGQIKEPILTVEGEEGRRLGSLQYKVFNNHIVLINPDY
                     ASANDDDAQEPLLVYYVDDQTVIVVPITQSVFHQGQYAPALVAQAELVDNRYTVQAQP
                     STDYPGRYATQVHDAHGYELVLQLDNVSIIALDDRVIVDVKEVVGKAGDAHQTHSAFI
                     ARNDTIVVDETCVFKIEQVTRTEGGYEVTFSVRDLYPELDGNDDDVLLEQGRDNPLLV
                     ERPVKGNEDTRDENTRDENTRDPSTRSTRSGQAGGDLPVNGHREPVGRGDLPATIEHP
                     ETPTASSPRGDLRIGNDN"
BASE COUNT          435 a          500 c          386 g          433 t
ORIGIN      
        1 attgtcgttg cctatccgaa ggtcaccccg aggactactg gccgtggggg tctccgggtg
       61 ttcaattgtt gcagggagat cgccacgccc tacgggctcg cgatgaccat ttaccgggag
      121 atcgccaccc gcctgccctg agcgagtgga gcgagtcgaa gggtccctcg tgttttcgtc
      181 cctcgtgttt tcgtccctcg tgtcttcgtt ccccttcacg ggtctttcta ctaacaaagg
      241 attatcccgc ccctgctcaa gcagcacatc atcatcgttg ccatcgagtt cgggatacaa
      301 atctcttacg gaaaacgtga cttcatatcc cccctccgta cgcgtcacct gttcaatctt
      361 gaaaacgcac gtctcgtcca ccacaatggt atcgttcctg gcaatgaagg cagaatgagt
      421 ttgatgcgcg tcacctgcct tacccacaac ctctttcaca tcaacgataa cacgatcatc
      481 caaagcaata atactcacat tatccaactg tagcaccagc tcataaccgt gggcatcgtg
      541 aacctgcgtg gcataacgac ccggataatc tgttgatggc tgagcctgca ctgtgtatcg
      601 attatccaca agttcagctt gggccactaa cgccggcgca tattgcccct gatgaaaaac
      661 actctgtgtg atgggcacaa caatcactgt ctgatcatca acataataaa ccagcaaagg
      721 ttcctgcgcg tcatcgtcat tggcagaggc ataatcggga ttgatgagca caatatgatt
      781 gttaaagact ttatactgaa gtgagcccag tctccggcct tcttccccct caactgtgag
      841 aataggttcc ttgatctggc cgttgcgctc gatcatcaca cgtcttttcc tgccgcgcca
      901 atcggtcaac ggcatttcca ttttaccatt tacgagattg attttgtgac agtgatcatg
      961 ggccaaaagc cgaccatcat cggtaaactc aagctcaatg tggtcaccac ccggcatgat
     1021 caacaaacgg cgatcggcac aacccaaagg atgaggcaag agctttgtac cgacaggata
     1081 ctcacgcaac acatacttgg cctgctctcg aaaacacctc acagccagcg taatagacaa
     1141 gtcatctctg ctcaaacgcc attcatcagc agggagtggt tccgtcaaaa tgaattgtgc
     1201 atcaaataaa gctcggtgtg ccggatcaat cacaaacccc atgattcttt tgcgcagtgc
     1261 acttgtacgc gagactcgat cagcaccgag caaaacagtc agttcacacc catcaaaggc
     1321 agaccctttt ttgcccctgt aacgataggc cagcacatca ccgcaattgg gcaacaccca
     1381 caattcatca tttgcccgaa attgatttgc attttttttg cggtcctcag gagcctcgct
     1441 gatcaatgaa aatggcccac tttcaaccgt ctcattacgc atatcccatg tatgaatacg
     1501 accggaacgc tttgtacttt ctacatcttc aaacctgagg atgagcctgg tcccatctcg
     1561 cgcaatgatc acaggcttga aaaagtgggt cgtgccagat cttcggtctt ccggttgctc
     1621 caccttgtat cccgaagctt tcaaacctcg atactcaccc ttggaaacgg aaggtgttgt
     1681 ggtgccatca tcaacaccgg aaagtttgat gaccggcagg ggtttggtgg gttcgctaaa
     1741 actgccagcg ttgg
//
LOCUS       DATDUN010000493         1979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_607018, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000493 DATDUN010000000
VERSION     DATDUN010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607018"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VJC18_06850"
     CDS             complement(<1..1114)
                     /locus_tag="VJC18_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HLD45138.1"
                     /translation="MSIHQVKCQLGDHEVIIETGKMAKQAGGAVTVRCGDSIVLVTAT
                     SSLQSKDEVDFLPLTVEYLEKTFSAGKIPGGFFKREGRPTETAILTSRFIDRPIRPLF
                     PDHYYFETQVIATVLSASGEMPPDMLAIVGASSSLMVSDIPFKKPLAGCRVCRIDGDF
                     VINPSISALSKSDLDLVVAATDDAVVMVEGGAQEAKEEDLMAAIEFAHKALKPVIAAQ
                     VELQKMCGKAKRSVPQPETRADITQAVKGYTGKVKEALGIKIKQQRYTQLDVLKDELK
                     TKLITKESPYAHKLQLATEFENLKSDLMRGAIINDKTRIDGRGLTDIRTITSEVSVLP
                     RTHGSALFTRGETQALCVATLGSADDEQTIDGLLEET"
     gene            complement(1169..1438)
                     /gene="rpsO"
                     /locus_tag="VJC18_06855"
     CDS             complement(1169..1438)
                     /gene="rpsO"
                     /locus_tag="VJC18_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HLD45139.1"
                     /translation="MSVTNEVRQALIKKFQTHENDTGSPEVQIALLSERIRNLTDHFK
                     THEKDHSSRRGLIMLVNQRRRLLSYLKRINLGSYQKVVGELSIRG"
     gene            complement(1687..>1979)
                     /locus_tag="VJC18_06860"
     CDS             complement(1687..>1979)
                     /locus_tag="VJC18_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45140.1"
                     /translation="IAAEAEAPQSYLSQMEGHMELIEANFQKMLQTQDAQEKNALLKQ
                     HWSLLDKHLELMNQMIDNERDLGLKSTKRRMKYRIHALLDMFKKTQNYLKIQ"
BASE COUNT          453 a          469 c          504 g          553 t
ORIGIN      
        1 aagtttcttc caacagacca tcgatggttt gttcatcatc ggcagaaccc aacgtggcca
       61 cgcacagggc ctgagtctca ccgcgcgtga acaaggcgga tccgtgcgtt cggggcaaca
      121 cgctcacctc gctggtgatc gttctgatat cggtgagtcc tctgccatca atacgcgttt
      181 tgtcgttgat gatggcgcct ctcatgaggt cggatttgag gttctcgaac tcagtagcca
      241 gttgcaattt atgcgcgtag ggggattctt tggtgatgag tttggttttg agctcgtctt
      301 tcagcacatc gagttgcgtg tagcgttgct gtttgatctt gatgcccaag gcttctttga
      361 ccttgcccgt gtagcccttg accgcttgcg tgatgtcggc gcgggtttcg ggttgcggga
      421 cagagcgttt cgccttaccg cacatttttt gcaattcgac ttgtgcggcg atgacgggtt
      481 tgagtgcctt atgcgcaaat tcgatggcgg ccatcaggtc ttcttccttg gcttcctggg
      541 caccaccctc gaccatcacc acagcatcgt cagtagcggc caccaccaga tccagatcgc
      601 tcttgctcaa tgccgagatg gacgggttga tcacaaaatc gccgtcaatc cggcacacac
      661 gacaaccagc caaaggcttt ttgaagggaa tgtcggacac catcaacgag gaactggcac
      721 ccacgatggc cagcatatcg ggaggcatct caccactggc tgagagcaca gtggcaatga
      781 cctgtgtttc gaaataataa tgatcgggaa acaggggccg tatgggacgg tcaatgaatc
      841 gcgaagtgag gatggccgtc tcagttggcc tgccttctct tttgaaaaaa ccacccggga
      901 tcttgcctgc cgaaaaggtt ttttcgaggt attctacggt gaggggcaaa aaatcaacct
      961 cgtctttcga ctgtaaggac gacgtcgccg tgaccagcac gatactgtcg ccacagcgca
     1021 cggtgacagc gccacccgcc tgttttgcca ttttaccggt ttcgatgatg acttcgtggt
     1081 cacccaattg gcatttgact tgatgaatag acatatttct ccttaaacag aaatgatcgt
     1141 aaaggacgat aaactaagga gacgttaatc agcctcggat gctgagctca ccgacaactt
     1201 tttgatagct gccaaggttg attcttttga ggtagctcaa caagcgacga cgttgattca
     1261 cgagcatgat caaacctctg cgtgaggaat gatctttttc gtgtgttttg aagtggtctg
     1321 ttaaatttct gatgcgttct gacaaaagcg ctatctgcac ttcgggagaa cctgtatcgt
     1381 tctcgtgtgt ttgaaatttc ttgatgagag cttgtctcac ttcgttggtc acagacattt
     1441 tcgtctccgg tctcccgtgc aaagtaagct cgctgtcatc agtcatcatt aaatcattga
     1501 tgcactaaaa cactttagtg aattaatgac tcaatgacac tggcaagcaa acctcatcac
     1561 aggtctagtg ttatcgtcta aattacacac aacagaaaac cgacaaaaca ctattgattt
     1621 gtcgcgttcc ctgatgggag acgctatttc gcacagatga taccgtaatg taaagtgaaa
     1681 tcctaattat tgtatcttga ggtaattctg cgtctttttg aacatatcga gcagggcgtg
     1741 aatgcggtat ttcatgcgac gtttggtgct tttgaggccc aggtcacgtt cattatcaat
     1801 catttggttc atgagttcga ggtgtttgtc caagagggac caatgttgtt tgaggagagc
     1861 gtttttttcc tgcgcgtctt gggtttgaag cattttttga aaattggcct caatgagctc
     1921 catgtgccct tccatttgtg acagatatga ttggggggct tcggcctcag cggcgatgg
//
LOCUS       DATDUN010000494         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_481483, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000494 DATDUN010000000
VERSION     DATDUN010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481483"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VJC18_06865"
     CDS             complement(<1..399)
                     /locus_tag="VJC18_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLD45141.1"
                     /translation="MKIEAYGLSDVGKKRTRNEDSFLVRSDLGLYVVADGMGGHSGGE
                     YASRLAVATIEEVISSMNTDPEATVISGVNSEETEFGDRLRYAIEVAGQKIFDQALYD
                     PDLKGMGTTITSVILDSHWAYVANVGDSRVY"
     gene            complement(474..1817)
                     /locus_tag="VJC18_06870"
     CDS             complement(474..1817)
                     /locus_tag="VJC18_06870"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HLD45142.1"
                     /translation="MHNTGDACAQKIKDRLAIKGVADFEVFVQERQGTTLESHDGHLR
                     LFEKKQSRAWAIRLQRNAREGFSYGSDFSSAAIDQSIKTAWDAMDCYTPDENHGFSSD
                     NRALEPQPFFDEHFEALTLSDKKQIISKITQTALNHDERVTAVKQARYEDEQEVTFIE
                     NSNGLKRSQQRTLFDVSAIAIAKDQLSEEMAQRFTARMYYSDIDPVHFGKVLAESALE
                     QLGAHRCKGYDGPVVLRGHVMATLLRYWSTSFFADSIHAGTSYLSGKQGQLVYSPSLT
                     LVDDPYNRQGSGCSLFDDEGVATHRTELVTQGVVTRFMTNEYWARKLRTSNTGNAKRL
                     NITQRPSVHAHNLLLTPGTMTQEQLYSQLDSGVVVTNLLGAHTINSVNGDFSLGIAGV
                     FIQNGRVKQALRSLVLSGNLHKLFANVHAVGSEQQFEGRYGGVSALIDRAVLTGR"
BASE COUNT          514 a          547 c          470 g          513 t
ORIGIN      
        1 atacacccgc gaatccccta cgttggcgac atacgcccag tggctatcga gaatgacaga
       61 tgtgatggtc gtccccatcc ctttgagatc gggatcgtac agggcttggt cgaatatctt
      121 ttgacctgcc acctcaatgg cgtaacggag acgatcccca aattcggttt cctctgaatt
      181 cacacccgat attactgtgg cttcggggtc cgtgttcatc gaagaaatca cttcctcaat
      241 ggtcgccaca gccaaacgcg aggcgtactc accaccagaa tgaccaccca tgccatcagc
      301 cacaacatac agtcccaaat cgcttctgac cagaaaactg tcttcattac gggttctttt
      361 tttaccaaca tcagaaagtc cataggcttc aatcttcata gcgtaccctt ataggcagag
      421 aatggtgatg cgtcaacgaa tattacaagc aattaaccaa acaaaacata tgattaccgt
      481 cccgttaaca cagcccgatc aatgagtgct gaaacacctc catagcggcc ctcaaactgt
      541 tgctcactgc ctaccgcatg cacattggca aatagtttgt ggaggttccc gctcaaaacg
      601 agggaacgca atgcctgttt gacccgaccg ttttgaatga agactcccgc gatccccaat
      661 gaaaaatcgc cattaacact gttgatggtg tgggcgccca acaagtttgt caccacaacc
      721 cctgagtcca attgggagta caattgctcc tgagtcatgg ttcctggcgt gagtaacaaa
      781 ttatgcgcgt gtacggacgg tctctgtgtg atgttcagcc tcttggcatt ccccgtgttg
      841 gacgtgcgta acttgcgagc ccaatactcg ttggtcatga atctggtgac gacgccttgc
      901 gtaaccaatt cagtacgatg ggtcgcgaca ccctcatcat caaacaggga acatcccgag
      961 ccttgtctgt tgtagggatc atccaccagc gtaagggagg gtgaataaac caattgcccc
     1021 tgtttgccag ataaatacga tgtgcccgca tgaatactgt cggcaaaaaa actggttgac
     1081 cagtaacgga gcaaggtggc cattacgtga cctcgtaaca ccacaggccc atcatacccc
     1141 ttgcacctgt gcgcaccaag ctgttcgagg gcactctcgg ccaaaacctt tccgaaatga
     1201 actggatcaa tgtcggaata atacattctg gcagtaaaac gctgagccat ttcctcactg
     1261 agttgatctt tggcaatggc gatcgcgctc acatcaaata gggttctctg ttgagaccgt
     1321 ttaagcccat ttgaattttc gatgaacgtc acctcttgct catcttcgta ccgcgcttgt
     1381 ttgactgccg tcacacgttc atcgtggttc agcgcggttt gagtgatttt ggaaatgatt
     1441 tgctttttat ctgagagcgt gagggcctca aagtgctcat cgaaaaaagg ttgcggttca
     1501 agagctctgt tgtctgacga aaatccatgg ttctcatcag gcgtgtaaca gtccatcgca
     1561 tcccaggccg tctttatgga ctgatcaatg gccgcacttg aaaaatcgga accatacgaa
     1621 aagccttcac gcgcatttct ctgtaacctg atggcccaag cccttgattg ttttttttcg
     1681 aaaagccgca ggtgcccatc gtgactctcg agcgtggttc cctgtcgttc ctgaacaaaa
     1741 acctcaaagt cagcaacacc tttgatagca aggcgatcct tgattttttg cgcgcacgca
     1801 tcccctgtat tgtgcatgag aatcgtacta atacaccacg acaatttgac aagagatttg
     1861 tagggtgtgc gacatagaag taggtaaatt tgattttgca cccccccgcc tgttgtaagg
     1921 cgggggtgcc cgaagggcgg gggtggtaga aaagatggtc atttcctgtc tctcagcttt
     1981 tcacgatact acctccccac ccttcgcaaa agctcaggga gcccctcctg tcaacaggag
     2041 gggc
//
LOCUS       DATDUN010000495         1850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_314040, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000495 DATDUN010000000
VERSION     DATDUN010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314040"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VJC18_06875"
     CDS             <1..1143
                     /locus_tag="VJC18_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45143.1"
                     /translation="AVELFSPRFSRLVLREDMPAYSFQREGVTEAVLTEADGVIAALC
                     AQGNMGVKRKSDPQPRVAKLGRASLVLSAAQHKIELPWIQAFERGMRLVRSANIEQEL
                     AIEIVKAMYQAMRPSSEGRSNRHIPVLNAYVELVAYVSTYVFLNRALGNVAMASAISL
                     LSCCMSDIIMEEGAGSERGAAFDFLSAAYLVPYLLFNRTVTHTEVFAGDIEPSIAFDQ
                     CPKDSAQAILLGCAAVLAKLNEINLEYNTPVDRRIHADSLHEIMELVQECDGIASRLA
                     AQNKLPPDVVLVQLWVKAKIAFKESQMAVTDADVAAKRSEMISYLDEARRWRSEMCGD
                     LTLEDVGLQHPGLLFNPVMSALCDEFMQAGIDISGTPNPGVLVDMF"
BASE COUNT          497 a          367 c          476 g          510 t
ORIGIN      
        1 gccgtagaat tgttttctcc aagattttct cgattggttt tacgcgaaga catgccagcc
       61 tatagttttc agcgagaagg tgtcactgag gctgtgttaa ccgaggctga tggcgtgata
      121 gctgctttat gtgcacaggg aaatatgggt gttaagagga aaagtgatcc acaaccacgc
      181 gtagcaaagt tgggcagggc aagtctggtt ttatcggcgg cacagcataa aatagagttg
      241 ccgtggatac aggcttttga gcggggtatg cgtttggtta gatctgcaaa cattgaacag
      301 gaactggcaa tcgaaattgt taaagccatg tatcaggcga tgcgcccaag tagcgaaggg
      361 agaagcaaca gacacattcc cgtattgaat gcctacgtcg agcttgtggc atatgtatca
      421 acttatgttt ttttgaatcg ggcactaggg aacgttgcta tggcttcggc tatttcgctg
      481 ttgtcatgct gcatgagcga tatcatcatg gaagagggcg cgggcagtga gcgaggggcc
      541 gcgtttgatt ttttgtctgc tgcttattta gtaccctatt tgctgtttaa tcgaacagtg
      601 acccatacag aagtgtttgc tggtgatatt gagccgtcca ttgcttttga tcaatgtcct
      661 aaagattcag cacaggcaat tttgctgggt tgtgctgctg tgttagcaaa actgaatgaa
      721 attaatttgg aatataacac acctgttgac agacgcatcc acgctgattc tttacatgag
      781 ataatggaat tggtgcagga atgtgatggt attgcgagcc gactagctgc acaaaacaaa
      841 cttcctcccg atgttgtgct ggtgcagtta tgggttaagg caaaaatcgc gtttaaagaa
      901 agtcaaatgg cagtgacaga tgctgatgtt gcagccaaac gttctgagat gatatcttat
      961 cttgatgaag ccaggcgttg gcgaagcgag atgtgtgggg atttgaccct tgaagacgtg
     1021 gggttgcaac atcccggact gctttttaat cctgtcatgt ctgccttgtg tgatgaattc
     1081 atgcaggctg ggattgatat ttctggcacg ccaaatccag gggtgctggt tgatatgttt
     1141 tgattttcgt gttgggaagt gctttagagg cgttggggag gcagcatgat tacccgatgt
     1201 caaagtattg ccccgacagc gcccgatttt ttttcttccg aaagattttc tgatcaaatt
     1261 atcaagagcg gcgacatgtt ttttctatgg gataaagttg atcgcagcca ggatggttca
     1321 gtgcgccccc cttggtttgc tgcccacaaa gctgccaata tatggtaatt aggaacttta
     1381 ttaaaataaa aaatcgatag caaccaaaaa cggacccaaa gcggccacaa ttttcgggca
     1441 ttttcgtgtt gagctgtgac aacaaacaaa gacaaaaaaa acatcacgcg atgtcattct
     1501 gaaagcgaaa aaatataaaa aacggggctt gaagaaaagg aaaaaaaaag gggaaaaaga
     1561 aaaaggggtc aagtctctgg ttgactagtt gctcaacaaa aaaactaact agtcaaccag
     1621 agacttgacc ccttctcaac tgaccccttc tcaacgtctt gagccagata atgtgggtgg
     1681 cacttttttg tccgattgtt gcttgccccg tgacgaaact ttacggggac tggcaccttt
     1741 aaagatcctc aatgatgagg gttctgtggc actcagacgc ctcactttcc acacaggtca
     1801 gggagacctt gcccttcgcc cgtctcatga tttccagaag ctgttctttg
//
LOCUS       DATDUN010000496         1106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_26944, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000496 DATDUN010000000
VERSION     DATDUN010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26944"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..>1106)
                     /locus_tag="VJC18_06880"
     CDS             complement(199..>1106)
                     /locus_tag="VJC18_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLD45144.1"
                     /translation="VVVLGARFDDFVIGNHAKFCAEATLVHVDIDASEINKIKDAHIP
                     IQGDVKLVIKAFNEVITKKLSIQPWIEQVLLWKAEFAHQYPKPKETIVPQDVIVKLKK
                     QVNDDVIVSVGVGQFQLWTAQLFGFKSPRTFLSSASLAAKGFGLPAAIGAQMAYPKRQ
                     VIDIEGDGNFLRNIQELQTLKALDIPIKCIVFNNQHMGMVALWENKTQKNLSGQTFLG
                     ETDFVAIANVFGVKGERICRTDQIEAALQRLLAYEGPYILDVQYPYAEASHGHVSPMI
                     PGDGSYLDTIMWDGTTLREYLQGGK"
BASE COUNT          272 a          315 c          236 g          283 t
ORIGIN      
        1 ctcctgacaa caggagtggg ggtactcaat tcaaatcacc cacttttatg tcgtacaccc
       61 cgctatcggc agcagggctt ctgtctattt gataaaaact gacaatctcg tcaaccacat
      121 cgccatccca aaccactcga accgtctagg ccaattgcac cttcccatat ttcttcattt
      181 cctgattgct tcattggttc atttcccacc ttgcaaatac tccctcagcg tcgtcccatc
      241 ccacatgatg gtatcgaggt acgaaccatc gcccgggatc atgggactca catgaccatg
      301 agaagcctct gcgtaagggt attgaacatc gagaatgtag ggcccctcat acgcaagcaa
      361 cctttgcagg gccgcttcga tctggtctgt gcggcaaatc ctctctcctt taacgccaaa
      421 gacattggcg atggccacaa aatcggtttc ccccaaaaag gtttgacccg acagattttt
      481 ttgggtcttg ttttcccaca aagccaccat ccccatgtgc tgattgttga agacgatgca
      541 cttaataggg atgtccaacg ccttgagggt ctggagttcc tgaatgttcc tcaaaaaatt
      601 tccgtctccc tcgatgtcga tcacctgcct cttgggatag gccatctgtg cgccaatggc
      661 cgctggcaaa ccaaaaccct tcgcggccag tgaggctgag cttaaaaatg ttctcggaga
      721 tttgaaccca aacagttggg ccgtccataa ttgaaactgc ccaacgccaa cggacacaat
      781 cacgtcgtcg ttaacttgtt ttttgagctt cacgatcaca tcctgtggca caatggtctc
      841 tttgggcttt gggtattgat gtgcgaactc agccttccac aacaatacct gctcgatcca
      901 aggctgaatg gaaagcttct tggtgatcac ttcgttgaag gccttaatga ccagcttcac
      961 gtcaccctga atggggatgt gggcatcctt gatcttgttg atttcggagg catcgatatc
     1021 cacatgcacc aaagtggcct ccgcacaaaa tttggcgtga ttgccgatca caaaatcgtc
     1081 aaagcgagcc cccaacacaa ccacca
//
LOCUS       DATDUN010000497         1807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_481721, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000497 DATDUN010000000
VERSION     DATDUN010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481721"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VJC18_06885"
     CDS             complement(<1..493)
                     /locus_tag="VJC18_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45145.1"
                     /translation="MFPLIKKINVPLKNLLPHLQNRNCFWLDSACFDFQPVAFDEQHG
                     FSILAFDPTDEKIFPDPTSAEAFCRFFASANLQRGPVTDISLRFTGGWLGFLAYEAYQ
                     FNKLIPFGPSHHKQYPLAIFKYYDTFFYKDHRSDKTLFVSFSPMAQKKWAGLQRQINQ
                     AARS"
     gene            592..1623
                     /gene="mltG"
                     /locus_tag="VJC18_06890"
     CDS             592..1623
                     /gene="mltG"
                     /locus_tag="VJC18_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HLD45146.1"
                     /translation="MLKAFRCLLIILSALVFILGGLGAFRAHQFAQYANPSEVSVVIN
                     KGASVKKIADQLKEQNIIRDALLFEAFVRLFSKGRFIRAGEYEFAQGLKLRGIVERLL
                     CGKVLRHKLTIPEGFRASEICALLVAKKLTPADVCLARVQDIGFLKEPAGATSLEGYL
                     FPETYFYERDDTLVSLLEQMTQLFYQKVENALTRSDRQTTRPLHQLVTLASIIEKETA
                     VAQERRLIAGVFVNRLDRGMLLQSDPTVIYGIDNYDGNIRKSDLEKDTPYNTYTRTGL
                     PSGPICNPGLASLEAAINPERTPFLYFVAKGDGSHHFSETLTEHNRAVSEYQRRRPKA
                     NDTTADKGE"
     gene            1626..>1807
                     /locus_tag="VJC18_06895"
     CDS             1626..>1807
                     /locus_tag="VJC18_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45147.1"
                     /translation="MQYLVYDIESVIDKKLLNQVLFDGKGLSDEEAYLQHVKDITKDE
                     KSFVNPSFHIPICLAC"
BASE COUNT          506 a          402 c          454 g          445 t
ORIGIN      
        1 aggaccgcgc cgcttgattt atctgtctct ggagcccagc ccactttttt tgagccattg
       61 gagaaaaaga cacaaagagg gttttgtcgc tacgatggtc cttgtagaaa aaggtgtcgt
      121 aatatttaaa aatcgccaag gggtattgtt tatggtggct tgggccaaag ggaattaatt
      181 tgttgaactg atacgcctcg taggccaaaa aacccaacca gccaccagtg aagcgtaaag
      241 aaatatccgt aacagggcct cgttgaaggt ttgctgaggc aaagaaacga cagaaggcct
      301 ctgctgaggt tggatcagga aagatttttt catcggttgg gtcaaaggca agaatagaaa
      361 aaccatgctg ttcatcgaaa gcgacaggct gaaagtcgaa acaagccgaa tccaaccaaa
      421 aacaattcct gttttgtagg tgcggcagca ggtttttaag agggacattg attttcttta
      481 ttagcgggaa caagggcatc agtctaggga gttttggacg ccgtttcaag tgcaataaag
      541 ggcttagaaa acaattgcca ccaaacaaaa acttcgttac caatggggcg aatgttaaaa
      601 gcttttcgat gccttctcat tatcctgtcg gccttggttt ttatcctagg gggtttgggc
      661 gccttccgcg cgcatcaatt cgcacaatac gcaaacccca gtgaggtttc tgtggtgatc
      721 aacaaagggg cctcagtgaa aaaaatcgcc gatcaactca aagaacaaaa tatcattcgt
      781 gatgctttgt tgttcgaagc ctttgtgcgc cttttttcca agggaagatt tatcagggcg
      841 ggagaatacg agtttgcaca gggcctgaag ttgcgaggga ttgttgagcg ccttctttgt
      901 ggaaaagtgc tgcggcacaa gctcacaatc cctgaggggt ttcgggcaag cgaaatctgc
      961 gccctgctgg ttgcgaaaaa gctgacgcca gcagatgtct gtttggcaag ggttcaagac
     1021 attggttttc tgaaagaacc cgctggcgca acctcattgg agggatacct gtttccagaa
     1081 acctattttt acgagagaga cgacaccctt gtgtctcttc tcgaacagat gacacagctg
     1141 ttttatcaga aagttgagaa cgccctcacc cggagcgatc gccagacgac aagaccgctt
     1201 caccagctcg tcactctcgc ctcgatcatt gaaaaagaaa cggcagtggc acaagaaaga
     1261 cggttgatcg caggtgtttt tgtcaatcgc cttgaccgcg gcatgttgtt gcagagcgac
     1321 cccacagtga tttatggaat tgacaactac gacgggaata ttcgtaaaag cgatttggaa
     1381 aaggacacgc cctacaacac gtatactcgt acaggtttac cgtctggccc catttgtaat
     1441 cccgggttgg cttccctgga ggccgcgatc aatcccgaac gaacgccgtt tttgtatttt
     1501 gtagcaaaag gggacgggag ccatcatttc tcagaaacgc tcacagaaca caaccgagcc
     1561 gtcagtgaat atcaaagaag gcgcccgaag gcaaacgaca caacggcaga caagggagag
     1621 tgactatgca atacttggta tacgatatcg aatcggtcat tgataaaaaa cttctcaatc
     1681 aggtgttgtt tgatggcaag ggcctgtccg atgaggaggc ttatttacaa cacgtcaagg
     1741 atatcaccaa agatgaaaaa tcttttgtga atccctcttt tcacatcccc atttgtttgg
     1801 cttgctt
//
LOCUS       DATDUN010000498         1109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_107831, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000498 DATDUN010000000
VERSION     DATDUN010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107831"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="dnaX"
                     /locus_tag="VJC18_06900"
     CDS             complement(<1..1028)
                     /gene="dnaX"
                     /locus_tag="VJC18_06900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HLD45148.1"
                     /translation="MHLALARKYRPQNFLEVLGQGPVVKTLQNAIENNRLHHAYLFSG
                     VRGVGKTSMARILAKSINCEKGPTITPCQVCGPCVAITQGNSLDVMEIDGASHNSVEN
                     VRDLREQVKYLPTSGKYKIVIIDEVHMLTGPAFNALLKTLEEPPQHIIFIFATTEPHK
                     LPVTILSRCQKYDFRKLNTTTLQAHLKNIAMNENVEISDEALRLVTQCAQGSVRDGVS
                     LLDQVLSYQTADITADSVSEILGLGDRVLTQKMFTTIIKEELSSGLELLQQVDERGVD
                     LKLFAEDLLLCFRHLILLESTGKVPPELSQAESEHLHSLAGQLDLSLLLAQYQILFKG
                     IQELAHSE"
BASE COUNT          283 a          296 c          260 g          270 t
ORIGIN      
        1 aattcggaat gagccagttc ctgaatcccc ttgaacaaaa tttggtactg ggccagcaac
       61 aaagacaaat ccaactgacc cgcaagtgag tgtaaatgtt cggactccgc ctggctgagt
      121 tccggcggta ccttcccggt cgattcgagc agaatgagat gacggaagca gaggagcaaa
      181 tcctcggcaa acagcttgag atcaacgcca cgctcatcca cttgttgcaa cagctccaat
      241 cctgacgaca attcctcttt gatgatggtc gtaaacattt tctgcgtgag cacccgatcg
      301 cccaagccca aaatctcgct cacgctatct gccgtgatat cagcggtctg ataactcaac
      361 acctgatcca gcagggaaac gccatcacga acagaaccct gcgcacactg cgtcaccaga
      421 cgcaaggcct catcggagat ttccacattt tcgttcatcg caatattttt gagatgcgcc
      481 tgcagggttg tcgtgttcaa tttgcgaaaa tcgtatttct gacaacgact caagatggtc
      541 acagggagtt tgtgaggttc tgtggtggca aaaataaaaa tgatgtgttg aggaggttcc
      601 tcaagcgtct tgagcaaggc gttaaacgct ggccccgtaa gcatgtgcac ttcgtctatg
      661 atgacgattt tgtatttgcc ggacgtgggc aggtacttga cctgttctct caaatctctc
      721 acgttctcta ccgaattatg gctggcacca tcaatctcca tcacgtccag ggaatttccc
      781 tgcgtgatcg ccacacaggg cccacatacc tgacacggag taatcgtagg gcccttttcg
      841 caattgatgg acttggccaa gatcctggcc atcgaagttt tgcctacccc tcgtaccccc
      901 gaaaaaagat acgcgtgatg gagccggttg ttctcgatgg cattttggag ggtcttcacc
      961 acaggcccct gtcccaggac ttcgagaaaa ttttgaggtc gatattttcg ggcgagtgcc
     1021 aaatgcataa aattgagacc ctcttttaga tagcaccacc ggcaatgtca acacattagg
     1081 ggcaacgcaa aaaacaccct cttttcgtc
//
LOCUS       DATDUN010000499         1034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_521900, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000499 DATDUN010000000
VERSION     DATDUN010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521900"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..>1034)
                     /locus_tag="VJC18_06905"
     CDS             complement(207..>1034)
                     /locus_tag="VJC18_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HLD45149.1"
                     /translation="IVILSLMFGVLLVIPIGGADMPTVVSILNAYAGLSAVAMGFVLD
                     NKLLITAGALDGSSGLILSIIMCKAMNRSFINVLFGAFGQTLKRTEEGEQKVYKAETT
                     EGVAQILEQASFVVIVPGYGMAVAQAQHKVRELYDLLKKQGITVKFAIHPVAGRMPGH
                     MNVLLAEAEIPYDDLVEMDDINPDMPQVDACLVIGANDVVNPAARNDAASPIYGMPII
                     AADRAKQVFAIKRSKNPGFAGIDNELYFADKTFMLFGDAKAVIGDLVRQLSSVSGTH"
BASE COUNT          267 a          272 c          247 g          248 t
ORIGIN      
        1 cgtcaacggg tccaaaggac cctagttcac cgtagcggaa ttgacttgtt gctcaatgag
       61 caacggaccg tagcccttga cacagttaac tagggttctt tgaacccgtt tttttgactg
      121 taatttgaca gcacccagta aaattacagt caaggccccg aggcggagtg ccgctgccca
      181 agtgatctgt gaacacttac gtggttttaa tgtgtgccgc tgacgctgga tagctgccga
      241 accagatcgc caatgacggc tttggcatcc ccaaagagca tgaacgtttt gtctgcaaaa
      301 taaagttcat tatcaatgcc cgcaaaacct gggttcttgc tacgcttgat agcaaagacc
      361 tgtttggccc gatcagcggc gatgattggc atgccgtaga tcgggctggc ggcatcattc
      421 cttgcagcag ggttgacgac atcattcgca ccaatcacca agcaggcatc cacttggggc
      481 atatccggat tgatgtcatc catctccaca agatcgtcgt aggggatctc agcctcggct
      541 aaaagaacat tcatgtgtcc aggcatacga ccggccacag gatgaatcgc gaatttgacg
      601 gtgattccct gcttttttaa gaggtcatag agctctctca ccttgtgctg agcttgggct
      661 acggccattc catagccggg gacaatcacc acgaaactgg cctgctccaa aatttgagcc
      721 acgccttcag ttgtttcagc cttgtagact ttttgctcac cttcctcggt tcgcttgagt
      781 gtttggccaa aggccccgaa cagcacattg ataaacgatc gattcatggc cttacacata
      841 atgattgata aaattaatcc agacgatcca tctagcgccc ctgctgtgat taacagctta
      901 ttgtcgagca caaagcccat agccacggct gaaagtccgg cataggcatt caaaatggaa
      961 acgacggtgg gcatatccgc cccgccgatg gggatgacta agagcacacc aaacataagc
     1021 gacaaaatga caat
//
LOCUS       DATDUN010000500         1825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_27121, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000500 DATDUN010000000
VERSION     DATDUN010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27121"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /gene="mrdA"
                     /locus_tag="VJC18_06910"
     CDS             complement(<1..1479)
                     /gene="mrdA"
                     /locus_tag="VJC18_06910"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLD45150.1"
                     /translation="MISGQEENLHYLHRKIPFIIVFVMIFVLTVLVRLYYLQVLRGEH
                     YEKLADETFVRIEEVVARRGAITDRFGKVLADTRTYYEIVLIPQYLDDQDKIINSLLQ
                     VLPLDRQDILNRLYEARFEPRFQPVVVVDDAPYNWVANLQEQLLPDYDSKDSFSLRGV
                     AVRSLPVRKYLYPELFSHALGYLTEIDKEALKKAKEKYADIYSLKDLVGAAGVEQGYE
                     LELKGRDGVFARVVDARGREVTTDADLNILAQRAMVKPQPGQTLKTTLSYDAQMAAYQ
                     AFQESGKKGTVVALDPNTGEVLVMFSSPGYDANRITKKVDKEYWKKINLDEDKYLFNR
                     AIQGTYPPASTYKVVALAAGIDSGAIDPDKARFTCHGGLHFGNRFFKCWTSHGTLPTL
                     LGLSKSCDVFFYNVGLAVGVDGLAKYARLFGFGATTGIEIPFEKPGLVPSTEWKLKRY
                     HEKWYESETLSVSIGQSYNLVTPLQNAVAVTKIANGGYKITPH"
     gene            complement(1476..>1825)
                     /locus_tag="VJC18_06915"
     CDS             complement(1476..>1825)
                     /locus_tag="VJC18_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45151.1"
                     /translation="VLAIFLSLIVVLIVIWHLHAQIYTESYLVQSLWTMIMNIIQQLL
                     LTFLIMRDWKLLVASFTLINILVNAGVMFLLTIPFFIAWDNWYGRWQKTLQGRRDVDP
                     TDSLRRKRYGDMT"
BASE COUNT          456 a          515 c          422 g          432 t
ORIGIN      
        1 atgcggcgtg atcttgtaac ctccattggc gatctttgtc acagccacgg cattttgcag
       61 gggtgtcacc agattgtagc tctgtccgat cgataccgac agcgtctcgc tctcatacca
      121 tttttcgtga taccgcttaa gtttccattc tgttgaagga accagtccgg gtttctcaaa
      181 cgggatttcg atacccgtgg tggctccaaa accaaacaac ctggcgtatt ttgccaaccc
      241 atccaccccc acagccagac ccacattgta aaaaaacaca tcacaagact tggacaaacc
      301 cagaagcgtc ggcaaggtcc catgactcgt ccagcacttg aaaaatctgt ttccgaaatg
      361 aagcccacca tgacacgtga atctggcctt atccggatca atggcacccg aatcgatgcc
      421 cgcggccaaa gccacgacct tgtaggtgga cgctggtggg taggtcccct gaatggcgcg
      481 attgaaaaga tacttgtcct cgtccaaatt gattttcttc cagtattcct tgtccacttt
      541 tttggtgatg cggttcgcgt cgtatcctgg tgagctgaac atcaccagca cctctcccgt
      601 gtttggatcc agcgccacca cagtgccttt tttgccagat tcctgaaagg cctgataagc
      661 cgccatctgc gcgtcatacg acaaggttgt tttgagagtt tgaccgggtt gcggtttgac
      721 catggccctt tgagccaaaa tgttcaggtc agcatctgtg gtcacctcac ggccgcgcgc
      781 atccaccacc cgggcaaaga caccatcacg ccccttgagt tccagctcgt acccttgttc
      841 cacaccagcc gcccccacaa gatccttgag actatagatg tcggcatact tttctttggc
      901 cttcttgagg gcctctttgt cgatctcagt cagataaccc agcgcgtgcg aaaacagttc
      961 ggggtagaga tattttctga ccggcaaaga acgaacagcc acgccccgaa gcgaaaatga
     1021 atctttcgaa tcgtagtctg gcaaaagttg ttcctggaga ttggccaccc aattgtaagg
     1081 ggcatcatcg acgaccacca caggttgaaa gcgcggctca aaacgggcct cgtacagacg
     1141 attgaggatg tcttgcctgt ccaacggcag gacctgcaac agactgttga tgattttgtc
     1201 ctgatcgtcc agatattgtg ggatcaaaac gatttcgtag tacgttctgg tatccgccag
     1261 cacctttcca aaacgatccg tgatcgcccc tctcctcgcc accacctctt cgatcctcac
     1321 gaacgtttcg tcggcaagtt tttcgtaatg ctccccccgc aaaacctgca ggtaatacag
     1381 tcgcaccagc accgtgagca caaaaatcat cacaaacaca atgatgaacg ggatcttgcg
     1441 atgaaggtag tgaagatttt cttcctgacc ggatatcatg tcatgtcccc gtaccgtttg
     1501 cgcctcaagg aatccgtggg atcaacgtcg cgccgtccct gcagggtctt ctgccaacgg
     1561 ccataccaat tgtcccaggc tatgaaaaaa ggaatcgtga gcagaaacat cacgcccgcg
     1621 ttcaccagaa tgttgatcag ggtaaacgac gccaccaaca gtttccaatc acgcatgatg
     1681 aggaatgtga gcaaaagctg ttggatgatg ttcatgatca tggtccataa cgattgaacc
     1741 agatacgatt ctgtgtagat ctgggcgtgc aggtgccaaa tcacaatcag gacaacgatc
     1801 agcgaaagaa atatcgccaa aaccg
//
LOCUS       DATDUN010000501         1518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_230173, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000501 DATDUN010000000
VERSION     DATDUN010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230173"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VJC18_06920"
     CDS             <1..369
                     /locus_tag="VJC18_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HLD45152.1"
                     /translation="PFRETANIKDGSNIMADMATQCFAGNAARGMTMIALHNGGGVGI
                     GKSINGGFGLLLDGKPETDDIIKSAMTWDVMGGVARRAWGRCPNAITVCEEFNTKHTD
                     SHVTIPYLADDALVEQLVSS"
     gene            605..>1518
                     /locus_tag="VJC18_06925"
     CDS             605..>1518
                     /locus_tag="VJC18_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45153.1"
                     /translation="MLGNKILSLPLLLLSGCAPEYELKAKDTSEPTDVDLPPHDILDL
                     CSIGNIYDYVYLNEKGGYDADSEGLITSASEGLDHQIETWSDYYTAEDFWSGAQPFTA
                     LLFGCHHRGLVPGEGLNYDYYRAQIAIIPGEKTAELIPALLVDDGDFCSPISDYFDYT
                     AFPALSVLACNTFSWLDYAGAANTGYQNSFYAGQGLYPDVCDDWGYASPESETSFDSI
                     RMSSYPDGASETVYEKRCATKDEGLASGSVPYEITRSVSENIEERSDDAYDRVMALAS
                     RSFHSTTGTEYVGGLLYCNGEHCESSVP"
BASE COUNT          384 a          322 c          404 g          408 t
ORIGIN      
        1 cctttcaggg aaacggccaa catcaaggat ggctctaaca ttatggcgga tatggccacg
       61 cagtgttttg ccgggaatgc cgcacgcggg atgaccatga tagcccttca taatggcggt
      121 ggggttggga ttggaaaatc catcaatggt ggttttgggc ttttgctgga tggtaaacca
      181 gagacggatg acatcatcaa atcagcaatg acgtgggacg tgatgggtgg tgtcgcccgc
      241 cgtgcgtggg gccgttgccc caatgccatc acggtttgtg aggaattcaa cactaagcac
      301 accgattctc atgtgaccat cccgtatctg gcggatgacg ctttggtgga acaattggtg
      361 tcgtcatgat ctgattgatt tgcgatcccc caaaatgatc aaacaacgct tgattcaagt
      421 tttaacggac tttttgcgaa gatgacgatg gcatatccaa caagaacaag ctgactagga
      481 ctttgttaat acagggctca gtaacatctc ctctgaatcc tgctgtgaat cccggtgctg
      541 acgaaattat gggaaatgcc atggatgaca actgtgacat gatcatcgag tagaagggaa
      601 aaatatgtta gggaataaaa tcttatcgtt gcccctttta ttgctttcgg ggtgtgcacc
      661 tgaatacgag ctcaaggcaa aggacacatc agaaccgacg gatgtggatt tgccgcctca
      721 cgacatattg gatctttgtt ctattggaaa tatttatgat tatgtttacc tgaatgaaaa
      781 gggaggttac gatgctgatt ctgaagggct gataacatct gccagcgaag gccttgatca
      841 ccaaattgaa acgtggtcgg attattacac cgcggaagac ttttggtctg gtgcgcaacc
      901 tttcacggcc ttgttgtttg gttgccatca tcgtggcctc gttcccggtg aaggtcttaa
      961 ctatgattat tatcgagcac aaattgccat aattcccggt gagaaaacag cagaactcat
     1021 tcctgctttg cttgtggatg atggggattt ttgcagtccg attagtgatt attttgatta
     1081 caccgctttc ccggcgttat cagtacttgc ttgcaacacg ttcagctggt tggactatgc
     1141 tggtgcggcg aataccggct atcaaaacag cttctatgcc ggacaaggtc tctatcccga
     1201 tgtgtgtgat gattgggggt atgcttctcc tgagtcggaa acctcgtttg attcaatccg
     1261 catgagctcc tatcctgacg gggcatcaga aacagtatat gagaaacgat gcgccacaaa
     1321 agatgaaggg ctcgcttcgg gcagtgtacc ctatgagatc acaagatctg tgagtgaaaa
     1381 tatcgaggag agaagtgacg acgcgtacga tagagttatg gccctggcaa gccgctcctt
     1441 tcatagtacg actggcacag agtatgtggg gggacttttg tattgcaatg gagagcattg
     1501 tgaaagctct gtgccctg
//
LOCUS       DATDUN010000502         1776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_275325, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000502 DATDUN010000000
VERSION     DATDUN010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275325"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..895)
                     /locus_tag="VJC18_06930"
     CDS             complement(107..895)
                     /locus_tag="VJC18_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HLD45154.1"
                     /translation="MSTRTTRRPVTLSAVVGFLNENLAVDRIADSSLNGLQVGFADDG
                     RNVSIKKIGLAVDASLTSFVLAKKVGCDLLIVHHGLFWSKDIPVTRGHYKRLEFLINN
                     QLGLYAAHLPLDAHDELGHNIQIAKRLGLKDVQPFGKYHGKMIGFSGRFSKPVSQKLL
                     LHRCESEFGAKCDFLAFGSKQIQTVGIVSGGGSSTMGEACAKGLDVLITGERVYSDYH
                     LAKDSEMNVLYGGHYFTEIPGLLALRDVLQKKLSISCEFLGVEQ"
     gene            complement(956..>1776)
                     /locus_tag="VJC18_06935"
     CDS             complement(956..>1776)
                     /locus_tag="VJC18_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HLD45155.1"
                     /translation="DENRVRNLVICIHPTHDVWVRDYGPLFVYRQGKQQTERLITKWQ
                     FNGWGGKYEEEFLNDNEIPRQIAKMTDSELISVEHVLEGGSIDVNGRGTLITSTDCLL
                     NDNRQTGVSRQKIEELFHRYLGIRQVIWAQGQIKGDDTDGHIDDAVRFVGPRQVVCIA
                     EKRAQDVNYESLRCLKESLAHIKDQDGEPIAVAELPMPNPVEFEGQRMPASYANFLIT
                     NKKILVPVYGCDQDQEALKIIGSFFPDRKAVGIDCCALVTGFGSIHCVSMHVPV"
BASE COUNT          465 a          485 c          377 g          449 t
ORIGIN      
        1 cgccgctatt ttgttgatga gatcattggg gagtttgtga atgagaggaa tcataacgat
       61 caaacaataa aaccatgaaa taatgaatca atgaagaaag aaaaatctat tgctcaacac
      121 ccaaaaactc acaggaaatg gagagttttt tttgcaacac atctctcaac gccaagagtc
      181 ccggaatttc ggtaaaataa tgcccgccgt acagcacatt catttcagaa tcttttgcca
      241 gatgatagtc gctgtacacg cgctcgccgg tgatgagaac atcgagccct ttcgcacacg
      301 cctcgcccat cgttgaagaa ccgccaccgg agacaatccc cactgtttgt atttgttttg
      361 acccgaatgc caaaaaatca catttggcac caaactcact ttcgcatctg tgcaaaagca
      421 gtttttgaga aacaggtttg gaaaacctgc cagaaaaacc aatcatcttc ccgtgatatt
      481 ttccgaacgg ttgcacatcc ttcaaaccca gtcttttcgc gatctggatg ttatgcccca
      541 gttcgtcatg cgcatccagg ggcaagtgtg cggcatacag tcccaattgg ttattgatca
      601 aaaactcgag tcgtttgtaa tgcccacgcg tgacaggaat gtcttttgac caaaaaagac
      661 cgtgatggac aatcagcaaa tcacaaccca ctttttttgc caaaacaaac gaggtgagcg
      721 aagcgtccac agccaacccg attttcttga tgctcacgtt cctgccatca tcggcaaacc
      781 caacctgcaa gccattgaga gaggaatcgg caatgcgatc aacagcgagg ttttcattca
      841 gaaacccgac cacagcactc aacgtcacag gtctgcgtgt tgttcttgtt gacatcatga
      901 ctccttatat gagctattct cgtccctcat ccctcgtttc ttcgtgtttt cgttctcaca
      961 ccggcacatg catactcacg cagtgaatgg acccaaagcc agtcaccagg gcgcaacaat
     1021 caatccccac tgcctttcta tcgggaaaaa acgacccgat gatcttcaga gcctcctgat
     1081 cctgatcaca gccataaaca ggcaccagaa tttttttgtt tgtgatcaga aaattggcgt
     1141 agctggcggg catgcgttgg ccctcaaatt caacaggatt gggcatggga agctctgcca
     1201 cagcgatggg ttctccgtcc tgatccttga tgtgggccag tgattctttc aggcaacgca
     1261 gggattcata attcacatct tgcgctctct tttctgcaat gcaaacaacc tgccgcggcc
     1321 ccacaaagcg caccgcatcg tcaatgtgcc catcggtatc atcgcccttg atttgcccct
     1381 gcgcccaaat cacctggcga attcccagat aacgatggaa caactcctcg attttctgcc
     1441 gcgacacacc cgtttgtctg ttatcattca gaagacaatc cgtggacgtg atgagcgtgc
     1501 ccctgccatt cacatcgatc gaaccaccct ccagcacgtg ttccacgctg atcagctcag
     1561 agtcagtcat tttggcgatc tgacgcggga tttcgttgtc gttcaaaaat tcttcttcgt
     1621 atttgccgcc ccagccattg aattgccatt tggtgatgag tctctcagtt tgttgcttgc
     1681 cttgacgata cacaaatagg gggccataat cgcggaccca cacatcgtgg gtggggtgaa
     1741 tacagatgac gaggttgcgc acccgatttt catcca
//
LOCUS       DATDUN010000503         1451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_522023, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000503 DATDUN010000000
VERSION     DATDUN010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522023"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VJC18_06940"
     CDS             <1..190
                     /locus_tag="VJC18_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45156.1"
                     /translation="VRSAGSSFHSLPQKRLELMSLSIVVIPAKAGIHALLKLWIPAFA
                     GMTVWEFVSKFNKAEFKS"
     gene            192..1319
                     /locus_tag="VJC18_06945"
     CDS             192..1319
                     /locus_tag="VJC18_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HLD45157.1"
                     /translation="MISIIPVSHHKQKKAFINFPYSLYQNDPHWVPPLKLERQEFLDP
                     KKNPFFLNAEGQLFLALREDQVVGRISAHINHLHNERYSEKTGHFGLFDAIDDGEVAR
                     ALFDAVCEWHGKRGMTQIAGPFNLSVNEETGLLIDGFESSPFPLMAHNYPYYQKLIED
                     QGFTKLKDLFAWAYSAERPVPEQAQQIADVVKTYPGLTVREVNMRHLKKDVAIIRDVF
                     NSAWSKNWGFIPWTEEELGKIAKDLKLILNPKLALIAEVDGQPAAISIAFPNYHEAIK
                     DLKGRLFPFGIFKLIYRLKTNKIKTARLALLGIKKEFRHDILAGLSVYLYSEMHRRGQ
                     ALGLTGGELSWTLEDNEKINRGIELMGGKVYKKYRVYGKPI"
BASE COUNT          384 a          330 c          362 g          375 t
ORIGIN      
        1 tgtacgctcc gctggctcct catttcactc cttgcctcaa aagcgtctcg aacttatgtc
       61 gctgagtata gttgtcattc cagcgaaagc tggaatccat gccctcctaa aattatggat
      121 cccagctttc gctgggatga cagtttggga gtttgtttca aaatttaata aagctgaatt
      181 taaaagttaa tatgatttct attattcctg tttcccatca caaacaaaaa aaggccttta
      241 tcaacttccc ctattccctc tatcaaaacg atccgcactg ggtgcctcca ctgaaactgg
      301 agcggcagga gtttttggac ccgaaaaaaa atcctttttt tctcaacgca gagggacagc
      361 tatttttggc cctgcgcgag gatcaggttg tgggacgcat ttcggctcac atcaatcatt
      421 tgcataacga gaggtattct gaaaaaacag gacactttgg tttatttgat gccatcgacg
      481 acggggaggt ggcgagggct ttgtttgatg ccgtctgcga gtggcacggt aaacgtggga
      541 tgacgcagat tgcggggcca ttcaatttga gtgtgaacga ggaaacgggg ctattgatcg
      601 acggctttga gtcatcaccg tttcccctca tggcgcacaa ttatccctat taccaaaaac
      661 tcattgagga tcaggggttc accaaactca aggatttgtt tgcgtgggcc tattctgccg
      721 agcggcctgt gcccgagcag gcacaacaga ttgcggatgt ggtcaaaacc tatccggggc
      781 ttacggtgcg tgaagtgaac atgcgtcatc ttaaaaaaga tgtggccatc attcgcgatg
      841 tgttcaattc tgcctggtcc aagaactggg gcttcatccc gtggaccgag gaggagcttg
      901 ggaaaatcgc gaaagatctc aagcttatcc tcaacccgaa gttggccttg attgccgagg
      961 tggacggcca gccagcggcg atctccattg cgtttcccaa ttatcacgaa gcgattaaag
     1021 acttgaaagg ccgactgttt ccctttggga tcttcaaact tatttatcgg ctcaagacca
     1081 ataaaatcaa aacggccaga ttggccctgt tgggaatcaa aaaagaattt cgacacgata
     1141 ttctggcggg actgagtgtc tatttgtact ccgagatgca ccgtcgcgga caggcgcttg
     1201 gccttacggg cggggagttg tcgtggacgc tggaggacaa cgaaaaaatt aaccgcggta
     1261 tcgagctcat gggtggcaag gtatacaaaa aatacagggt gtatgggaag ccaatctgaa
     1321 tatcgaatat cgaacaagga atttcgaatc cttcaacttt gttcaggaca gatgtcgaac
     1381 ttcgttatcc ctgcaaaagc agggatccag aataataata tatggattcc agctttcgct
     1441 ggaatgacaa c
//
LOCUS       DATDUN010000504         3786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_27199, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000504 DATDUN010000000
VERSION     DATDUN010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27199"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..843
                     /gene="tsaB"
                     /locus_tag="VJC18_06950"
     CDS             154..843
                     /gene="tsaB"
                     /locus_tag="VJC18_06950"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HLD45158.1"
                     /translation="MAIKSYGIWCCLDCSVRNVSVALIQDGTVLSSFNGFPPISQSED
                     LMRLIEGVFLGAGRKPADVRHVLFCSGPGSFTSLRIGLATLKGYFWTDDVVFCTASSL
                     LFRCYSAHQSGDRLTISLIRLGRKRVAAGFLECNEKAEEGNFSEKIFDESDFGQIVPV
                     DCECVHLTGDGVELLKPGVDLSRVSRLSPTDPHSVEAFWSIIGKGQFIKSDLNTVVLN
                     YLTQPDFGKSN"
     gene            complement(840..1604)
                     /locus_tag="VJC18_06955"
     CDS             complement(840..1604)
                     /locus_tag="VJC18_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45159.1"
                     /translation="MKKIIFLFPIILVLCLATATAFAQEDVEYIEPTASSNSIGGAVG
                     LGYTYDTRDMNRLALDTNIQLPYRFSYYSYFNFNSQMAVDGANFEIDNFYSEHNLWWN
                     PYEPIPVDLALQYVIAQGAGNDMLQLGLRWRLSDTVWIKNYCEMIHLTLSSQVHPLQT
                     DLDASAGWGFSFEHRWSLQILPERFSKRFVMSGFLDHYLNYGGPANGNHHSFVVENEL
                     AVRVWGNFSALAAYRFSDYDVRQHGLAFGAKYVVNF"
     gene            1711..1782
                     /locus_tag="VJC18_06960"
     tRNA            1711..1782
                     /locus_tag="VJC18_06960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1744..1746,aa:Arg,seq:ccg)
     gene            2052..2957
                     /locus_tag="VJC18_06965"
     CDS             2052..2957
                     /locus_tag="VJC18_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45160.1"
                     /translation="MKKYYLSCLFLFLILCPCLSFAQVDDESQAYDGGQSEIVDSQDV
                     NDVSADDPSGKDVSHSAAIPDDFTGTFAEDDPDSEVYQEHQIFGEDEAEDEEEEGEAV
                     DEGPTHLLKLEFESQVVVSDALAATPFLEISYTLKLEQEIEVVNRRFRTKAEAQVTTD
                     VVGTIAGNELFTCKLEVQLNQVEADIMARYTLVEETEETEAISQLAVQIKFARDGLME
                     DWFSNCLGTDGSILNTKGDREKYLLTTLDSVTPDLKGIVFESYDITTEVSQDIAAEPL
                     LIEDADTHEEFMLQGSGSIEVTPIE"
     gene            complement(2983..>3786)
                     /locus_tag="VJC18_06970"
     CDS             complement(2983..>3786)
                     /locus_tag="VJC18_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLD45161.1"
                     /translation="KTIQMLEDRKILQETLLVIVSDHGLSETTEHFDVGPWLEKEKKI
                     KTLYHTNIFKRKFDAASMVSGNGMCHLYFKDDKGWSGRIPHEDFVKDTDLLAELVARP
                     EVSLVITQAQDETIYFQNKKGCSHFSVDTTQNIIHYTFTQTDPLGIFNNKSINPEEGF
                     TFDKSLELTWDSEYPDVFVQMHQLFRSHRTGDIVLSANSGYDFRNRFEVPLHKASHGA
                     LLPEHMKVPLLMNYPIEAQYIRSVDVFPTLLKLMGKAIPSGLDGRCLVG"
BASE COUNT          940 a          822 c         1002 g         1022 t
ORIGIN      
        1 ccatacaagt ttaaactggc gctcatgcag gtggggttgg ttgtgttgat ggggttcatg
       61 ttgttcgtga cgatcaacga tgtcgacaac ctctggggtt ttgcgaatat tttccagaag
      121 ctcaaagggg tgttttgacc catgaaggat tctgtggcaa tcaaatctta tgggatctgg
      181 tgttgtctgg attgttctgt caggaatgtt tctgtggcgc tcatacagga tggcacggtg
      241 ctatcttcct tcaacggatt tcctcctatc tcacaatccg aagacctcat gcgccttatc
      301 gaaggagttt ttctcggggc aggtcgtaag ccagctgacg tgcgacatgt tttattttgc
      361 tctggcccgg gttcattcac gtcactacgg attggtttgg ccaccctcaa gggatatttt
      421 tggacggacg atgtggtgtt ctgtacggcg tcgtcacttt tgtttcgctg ttattccgct
      481 catcagagcg gagatcgctt aacgatcagt ttgattcgtt tgggtcgcaa acgagtcgcc
      541 gcaggttttt tggaatgcaa tgaaaaagct gaagagggga atttttcaga aaaaatattt
      601 gatgagtccg attttggcca gatcgtccca gtcgactgcg agtgcgttca cctgacgggt
      661 gatggcgtgg agttgctcaa acccggtgtc gatctttcac gcgtgtcgcg actcagcccc
      721 accgaccccc acagcgttga ggcattttgg tccatcatcg ggaagggaca attcataaaa
      781 agcgatttga atacggtggt gctcaattat ctgacacaac ccgattttgg gaaatcgaat
      841 taaaaattca cgacgtattt ggctccaaag gccaagccgt gttgcctcac atcgtaatca
      901 ctgaatcgat aggcggccag ggcactgaaa tttccccaca cgcgaacagc cagttcattc
      961 tcaacaacga atgagtgatg attgccgttg gcaggaccgc cgtagttcag gtagtgatcc
     1021 aaaaagccgc tcatgacaaa tctcttgctg aagcgttcgg gcaggatctg cagagaccac
     1081 ctgtgctcaa aagagaaccc ccagcccgcg gaggcgtcca ggtctgtttg cagagggtgg
     1141 acctggctgg acagggtgag gtgaatcatc tcgcaataat ttttgatcca gaccgtgtcg
     1201 gacaggcgcc acctgagtcc cagttggagc atgtcgttgc cagcgccttg ggcaatcacg
     1261 tattgcaggg ccaaatcgac agggatgggt tcgtagggat tccaccacaa attgtgttcg
     1321 ctgtaaaaat tgtcgatttc aaaattggct ccatcgacgg ccatctgaga gttgaagttg
     1381 aaataactgt aataactgaa acgatagggg agttgaatat tggtatccag cgccagacgg
     1441 ttcatatcgc gcgtgtcata cgtgtatccc aggcccacag ccccgccaat gctattggag
     1501 ctggcagtgg gttcgatgta ctcgacatct tcctgggcaa aggctgttgc ggtggccagg
     1561 cagagcacga gaataatggg aaataaaaat attatttttt tcataatgac tccttttgct
     1621 gatcatagaa gaagcaaaat catgagtaaa gtggaaacat ctcttattga cgttctgctt
     1681 ggcaattgat tattggtggc acgtattttt gtccttgtag ctcagtggat agagcagtgg
     1741 cctccgaagc catgtgcgca ggttcgattc ctgccaggga cgctgttaac atgcgggtgg
     1801 ttctgcgaag tctggggcgc aaagcgctaa acatgcccaa tggcatgttc atcgcattgc
     1861 acggttcgat tcctgccagg gacgcaatta actcgtggat gattctgtga aatctgggtt
     1921 ttttagaggt gtccttaatc caataatatc aatcagataa actgtgattg ccgcaagtag
     1981 tatctttctc ttacccatca attgtacgat aggagaaatt ctgataaaat aagttgtcgt
     2041 ttattcattg tatgaaaaaa tattatctat catgtctgtt tttgtttttg atcctttgtc
     2101 cttgtctgag tttcgctcag gttgacgacg agtcacaggc gtatgacgga ggacaatcgg
     2161 aaattgttga ttctcaagat gtgaatgatg tctcagcaga cgatccctcg gggaaagatg
     2221 tttctcatag tgccgctatt cctgacgatt ttacggggac cttcgcagaa gatgaccctg
     2281 atagtgaggt gtatcaggaa catcaaatat tcggcgagga tgaagcggag gatgaagaag
     2341 aagaggggga agctgtcgat gaaggcccca cacatttatt aaaacttgag tttgagtcac
     2401 aggtggtcgt gagcgatgcc ttggcggcga ctcctttttt ggaaatcagc tacacgctca
     2461 agcttgaaca ggaaatcgag gttgtgaaca gacgttttcg caccaaagcc gaagcccagg
     2521 tcaccacaga cgttgtgggt acgatcgctg ggaacgaatt gttcacctgc aaactggagg
     2581 tccaacttaa ccaggtcgaa gccgatatca tggcccgtta cacattggtg gaagaaaccg
     2641 aggaaaccga ggccatcagc caattggctg ttcaaatcaa gtttgcccgg gatgggctga
     2701 tggaagattg gttttcgaat tgtttgggga ctgatggcag tattctcaac accaagggcg
     2761 accgcgaaaa atacctgctg accacgctcg atagtgtcac tccagatctg aaggggatcg
     2821 tgttcgaatc ctatgacata actactgagg tttcacaaga cattgccgca gaacccttgc
     2881 tcatcgaaga tgctgacaca cacgaggagt ttatgttgca ggggagtggc tcgattgaag
     2941 tgactcccat tgaatgaagg ttcctgaagc acaaacagac aatcaaccaa ccaggcacct
     3001 gccgtctaag ccactgggga tggctttgcc catgagtttg agcagggtgg ggaacacatc
     3061 aactgaacgg atgtattgag cctcgatggg ataattcatc agcaaaggca ctttcatgtg
     3121 ttccggcaat agggcaccgt ggctggcttt gtgcaaggga acctcgaacc ggttgcggaa
     3181 atcgtacccg ctgttggccg acaggacaat gtcgcctgtt cggtgtgagc gaaaaagctg
     3241 gtgcatttgt acaaacacat cggggtattc agaatcccaa gtgagctcca agcttttatc
     3301 gaaggtgaag ccttcttcag ggttaatgct tttgttatta aaaataccca agggatctgt
     3361 ttgagtgaag gtgtagtgaa tgatgttctg tgtggtgtcg acagagaaat ggctacaacc
     3421 ctttttgttc tgaaagtaga ttgtttcgtc ttgcgcttgt gtgatgacca ggctgacctc
     3481 cggtcgggct accaattcgg ccaataaatc ggtgtctttg acaaaatctt catggggaat
     3541 acgacccgac caccctttgt cgtccttgaa atacaggtgg cacatgccgt tgcccgaaac
     3601 catggaggcc gcatcgaatt ttcgtttaaa aatattggtg tgatagagcg ttttgatttt
     3661 tttttctttt tcgagccaag gtcccacgtc aaaatgttcg gttgtttcac tcaggccgtg
     3721 atcactgacg atgacgagaa gggtttcctg tagaattttt cgatcttcca gcatttgaat
     3781 ggtttt
//
LOCUS       DATDUN010000505         1193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_522041, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000505 DATDUN010000000
VERSION     DATDUN010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522041"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VJC18_06975"
     CDS             <1..321
                     /locus_tag="VJC18_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLD45162.1"
                     /translation="LLAARTNNSDLVFTTGGTGIGPRDLTPDVVLPLLDKTVPGIMES
                     IRFKYGQNLPSALLSRSVAGVMGTCLVYTLPGSVRAVSEYLAEILKTLPHSLLMLWGI
                     DAHH"
     gene            complement(482..868)
                     /locus_tag="VJC18_06980"
     CDS             complement(482..868)
                     /locus_tag="VJC18_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD45163.1"
                     /translation="MSKQHILVIDSDPEIVDSLLGQLEQYGLEGCPVTSLEQAVRMVK
                     EQVPDVIIMDSGFAQEEGEYYFHLIKSVLPKGEKVPALRVMSVTQDQNLRTYAYEMGE
                     EETPLRPFDADSLKTLMADYLPVDDL"
BASE COUNT          310 a          305 c          266 g          312 t
ORIGIN      
        1 ctgctggcag cgcgcacgaa taacagcgac ctggtattca cgacaggtgg cacagggatt
       61 gggccgcgcg acctgacacc cgatgtcgtt ttgccattgc ttgacaagac cgttcccggg
      121 atcatggagt ccatccgttt caagtacgga caaaacctgc catccgccct gctgagccgt
      181 agtgtggcgg gagtaatggg cacctgcctg gtttacaccc tccccggcag tgtccgcgcc
      241 gtctctgaat atctggctga aattcttaag acccttccac acagtttgtt gatgctttgg
      301 ggcatcgacg cacatcattg acacgccggc aaaatcgtca tgatttatgg ttacggcatt
      361 gagagtccag attgaggttc atgattgcct cacggctcat taggatcaaa ggtagtttca
      421 aatcctgagg attcagggac tgcgaaagga tttctgctcg ttttaaggaa acctctaata
      481 gctacaagtc atcaacaggc aaataatccg ccatgagtgt cttgagtgag tccgcatcga
      541 agggacgcaa aggggtctcc tcctcaccca tttcgtatgc gtaagtgcgt aaattttgat
      601 cctgtgtcac actcatcacc ctgagggcag ggactttttc tcctttgggc aacacggatt
      661 tgatcaggtg aaaataatac tccccctcct cctgtgcaaa ccccgagtcc atgatgatga
      721 cgtctggcac ctgttccttg accatcctga cagcctgttc caaagaggta acagggcatc
      781 cttccagacc gtactgctca agctgaccca acaaggaatc cacaatctcg ggatctgaat
      841 cgatcacaag gatatgttgt ttgctcatga tgtctcccct tttgtgatgt gtcattagaa
      901 agtataacac aacacgacac ccagcagaac cctgtatttg ttggaacaaa caaaataatt
      961 ttgtcaatag ggtgacagat tttttagttt cgaaatgagt acagactttt ccgtaatatg
     1021 gacaaactca acaagcagtg aacagttcaa tgactaaccg tatacaaatt cacctgaatt
     1081 ccgcacttga cagggctcca ttcaggccaa accgctgaaa tcgctgaatc tctgcgttgc
     1141 tcaggaattt ttaaatcctc atgtattttc ctatacattc cggtttaaaa atc
//
LOCUS       DATDUN010000506         3079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_522047, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000506 DATDUN010000000
VERSION     DATDUN010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522047"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VJC18_06985"
     CDS             complement(<1..653)
                     /locus_tag="VJC18_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HLD45164.1"
                     /translation="MHGTGNNAHLPENAFRKLKPGEVYQPIVPASSNMPQWTFRAVLL
                     GIITAVIFSAAAAFLGFKVGQVIEAAIPIAIIAVGVGSVYRRRASLLENVIVQSVGAA
                     SGLIVAGAIFVLPALFILELPVDKFIAFMASLLGGVLGVLLLIPLRRYFVKEMHGEYP
                     FPEATATTEILVAGESVGTQAKILMTAAVVGGIYDFLQLHAKAFSETITTYTIPYVNT
                     "
     gene            868..2247
                     /locus_tag="VJC18_06990"
     CDS             868..2247
                     /locus_tag="VJC18_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HLD45165.1"
                     /translation="MTQEEYPFEPYLISVTRPINITTREERQKIIRDHHFNLFKIPSK
                     KVMIDLLTDSGTGAISNVQLAGIILGDESYAGSSSFDKMLSAIEDITGFKYVIPTHQG
                     RGAENVLHSTLIKEGDLIPGNTHFDTTKAHIEYRKGIAIDCTIDEAKDPLLQHPFKGN
                     IDLDKLEKVLKGTPKNKIPFVLITVTCNSGGGQPVSMANIQAVKALCHKQGLRVFFDM
                     ARYAENAYFIKTREPAYHDWTIRDIIKEMFKGSDGATMSAKKDAIVAMGGFLALRDEG
                     LYQQCSVYSILFEGYLTYGGMTGGTMETIAAGMYEGTEYHYLKARVGQVARLAQRMKD
                     LGVPVVEPAGGHAVYVNAGKMIPHITADNYPAQALGVAAYVHGGVRGVEIGTVLADRD
                     PITRQERHPHLELLRLAIPRRTLTDNHMNYVAETFHKVSQLKDSIRGLKIIREAPILR
                     HFTCEFDEI"
     gene            complement(2296..3024)
                     /locus_tag="VJC18_06995"
     CDS             complement(2296..3024)
                     /locus_tag="VJC18_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HLD45166.1"
                     /translation="MYIIKQSLVLPFGFIWFLLLFSLFSLKRAGLGKYFSLLCVLALL
                     VLSTPYVGQRLLVALQQDPPLTSFEHPAQAIVILGGGTRGFAPEYNAPSLQAVSLERV
                     LYGAYLFNRTSLPILVSGGDPLRTGINEADVMKKILEEQLLTPVKWTEDHSHTTYENA
                     KMSFEILSRGHITNIYLVTHARHMSRARFAFEQAGFDVIPAPTVFANQPSLSLINFVP
                     SVIGFNQSQNAFYEWFGQLYYRFR"
BASE COUNT          870 a          738 c          774 g          697 t
ORIGIN      
        1 gtattcacat agggaatcgt gtaggtcgtg atggtttcgg aaaaagcctt ggcgtgcaat
       61 tgcagaaaat cgtaaatgcc gccgacaacg gccgcagtca tgagtatttt ggcttgtgtg
      121 ccaacagatt cacccgccac cagaatttcg gttgtggcag tggcctcagg aaaagggtac
      181 tcgccatgca tttctttgac gaaataacgg cgaagaggaa tcagcaacaa cacgcccagc
      241 acgccaccta aaagggatgc catgaaggca ataaacttgt cgacagggag ttccagaatg
      301 aacaaagcgg gcagaacaaa gatggcaccc gccacaatca gaccagaggc cgctcctacg
      361 gattgcacaa tcacattttc cagaagagaa gcgcgtctgc gatacacgga acccacgcct
      421 actgcgatga tcgcaatggg gatggccgct tcgataacct gaccaacctt aaatcccaaa
      481 aaagcggcgg cagccgaaaa aatcacagcc gttatgatac ccagcaagac agctctaaac
      541 gtccactgcg gcatgttaga cgacgcgggg acgatgggct gataaacctc gccgggcttg
      601 agtttgcgaa aggcattttc tggcaaatga gcattgttac ctgttccgtg catgtaccct
      661 cctatgggaa ttccgagaaa gcgttttgtc ggtgtctcat gaaaaaagaa acagaacatc
      721 acgctcaaga caacatagcc cacataaaac ctcgctacac acccccgtgt gatatgggct
      781 ggattcagcg gcatagataa tttttgcaag gatttccaga agtttttata attgacatca
      841 aatacaagtg ttcataacag ttccctcatg acacaagaag aatatccttt tgaaccgtat
      901 ttgatttcgg tgacacgacc catcaacatt accacgcgtg aagaacgtca aaaaattatc
      961 cgcgatcatc actttaatct ttttaaaatc ccttccaaaa aggtgatgat tgatttgctt
     1021 accgattcgg gcacaggagc catcagcaat gtgcaattgg ctggtattat tcttggtgac
     1081 gaaagctatg cgggttcgag ttcgttcgat aaaatgcttt ccgccatcga ggacatcacg
     1141 ggattcaaat acgtgattcc cacacaccag gggcgcggag ccgaaaatgt ccttcactcg
     1201 actctcatca aggaggggga tttgattccc ggcaataccc actttgatac cacaaaggcc
     1261 cacattgaat accgaaaagg gatagcgatc gattgcacga ttgatgaggc caaagacccg
     1321 ttattgcaac atccctttaa gggcaacatt gacctagaca agctcgaaaa ggtcctcaag
     1381 ggaacgccca agaacaaaat tccgtttgtg ctcatcacgg tgacttgtaa ttctggtggt
     1441 ggacaacctg tttcgatggc gaacattcaa gcggtaaagg ccttgtgcca taagcagggg
     1501 ttgcgggtgt tctttgatat ggcacgctat gccgaaaacg cctatttcat taaaacgcgc
     1561 gaacctgcct atcacgactg gaccatcagg gacattatca aggagatgtt taaaggctca
     1621 gatggggcta ccatgtcggc caaaaaggat gcgattgtgg ccatgggagg ttttttggcg
     1681 ctcagggatg aagggcttta tcaacagtgt tccgtctaca gcatcttgtt tgagggctat
     1741 ctcacttatg ggggcatgac tggcgggacc atggaaacga tcgccgcagg catgtatgaa
     1801 ggcacggagt accattatct caaggcgcgc gtgggacagg tcgcacgcct ggcccagagg
     1861 atgaaggatc taggtgttcc tgttgtggag cccgcggggg gacacgctgt gtatgtgaat
     1921 gcggggaaaa tgattccaca tattacggcc gataattatc ctgcccaagc gctcggggtt
     1981 gcggcctatg ttcatggtgg cgtacggggc gttgagatag gcactgtgtt ggcggaccgt
     2041 gaccctatta cccggcaaga gagacaccca cacctggagt tactacgact cgccatcccc
     2101 agaagaacgc tcacagacaa tcacatgaac tatgtggccg aaacctttca caaggtaagc
     2161 cagctcaaag acagcatcag agggcttaaa attatccgcg aggcaccaat cttgcgccat
     2221 tttacctgtg agtttgacga aatttgaaac cagtccttcg acaatttaaa ttttatctag
     2281 tgacacagct caggactagc gaaaccgata atagagctgc ccaaaccatt catagaacgc
     2341 attttgactc tgattgaaac caatcacaga gggcacgaaa ttgatcagac tcaaagaggg
     2401 ctggtttgca aaaacagtgg gtgctgggat cacatcaaaa ccggcttgtt caaaggcaaa
     2461 gcgtgcgcgc gacatatgtc tggcatgagt caccagataa atgtttgtga tgtgcccgcg
     2521 cgatagaatt tcgaaactca tcttcgcgtt ttcataggtt gtgtgagaat gatcctcggt
     2581 ccatttgaca ggcgtgagca attgctcttc cagaatcttt ttcatgacat cggcctcgtt
     2641 aatgcccgtc ctcaggggat ctcccccact taccaaaatg ggaagcgagg tgcgattaaa
     2701 caaataggcg ccgtaaagga ctcgttccag cgaaacggct tggaggcttg gtgcattgta
     2761 ttcaggggcg aacccccgag tgccgccgcc caaaatcaca atggcctggg ccggatgttc
     2821 aaaacttgta agcggaggat cctgttgcag ggcaaccaac agcctctgtc caacgtaggg
     2881 ggtgctgagg accagtaggg caagcacaca caacaatgaa aaatattttc ccagccctgc
     2941 tcgtttgagt gaaaagagtg aaaacagcaa caaaaaccag ataaagccaa agggaaggac
     3001 cagggattgt ttgatgatgt acatggcatc ctttccacca ttttttacga taaggcaaat
     3061 gcttcatttg ttgcgcaaa
//
LOCUS       DATDUN010000507         1792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_192574, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000507 DATDUN010000000
VERSION     DATDUN010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192574"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VJC18_07000"
     CDS             <1..322
                     /locus_tag="VJC18_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HLD45167.1"
                     /translation="TGCDAVMTGRAAFHAPWIFRDFIDGNDQVPSPQQIEGMIFTHYD
                     LAKKYYGDAQGIKLMRKFICSYTKGLRDGAKLRNDLVRLTDWAIIEKIIRGFFGGAPM
                     AHSG"
     gene            421..702
                     /locus_tag="VJC18_07005"
     CDS             421..702
                     /locus_tag="VJC18_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HLD45168.1"
                     /translation="MAFEYDAKKSAQNEQKHGVSFAEAINLWDTTHVIVPAKHVAGES
                     RFAILGKLAGKMTVAIYTLRGEAIRIISCHRADKKLERIYEKLIKEKQG"
     gene            671..952
                     /locus_tag="VJC18_07010"
     CDS             671..952
                     /locus_tag="VJC18_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45169.1"
                     /translation="MKNSSKKNKVKKISDTVFDKMFDAGEDISAYLDTTAAIVVQRVN
                     VDFPQWMIKLLDNQANQLNVSRQAVIKMWIRDRLELVYGRQVFHTRPTA"
     gene            complement(1094..>1792)
                     /locus_tag="VJC18_07015"
     CDS             complement(1094..>1792)
                     /locus_tag="VJC18_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45170.1"
                     /translation="FGRLPIYRQVVFKLAQHHYGITREDLFRAVGLKTGGTAQKRLDE
                     LEEAGFITKFVPYGKQYDVHYRITDEYSLCYARWIHPLSKGVIASENYWLKKSTSPEW
                     SSWAGYAFENVCYKHLTQIARAISADHTAYVAGPWQCFGGECLKDGGAQVDLLFDRDD
                     GVINLCEIKCAKNEFVIDKEYAQNCQNKIQVFKEKTKTEKDVLLTFITLSGVKNNAYK
                     KAMVDSEVVLRDLF"
BASE COUNT          512 a          440 c          389 g          451 t
ORIGIN      
        1 gaccggctgt gacgcggtca tgacgggccg cgccgcgttt catgccccct ggattttcag
       61 ggattttatt gacgggaatg atcaggtccc ctcaccacag caaatcgaag ggatgatttt
      121 tacacactac gacttggcaa aaaaatacta cggcgatgct cagggcatca agctcatgcg
      181 caaattcatc tgttcttata ccaagggatt gcgtgacggc gccaaactca gaaacgacct
      241 cgtcagattg accgactggg cgatcattga aaaaatcatt cgggggtttt ttggtggtgc
      301 cccgatggca cacagcgggt aaatcacaac ttcgttgccg acagttagcc catcgctgaa
      361 tggcgcctgt ttgatattgc gcttgaccca aaatcgctca taatggtata agtcttatat
      421 atggcctttg aatatgatgc caagaaaagc gcacaaaacg aacaaaagca tggagtgagt
      481 tttgcagaag ccatcaacct gtgggataca acccatgtga ttgttcctgc aaaacatgtc
      541 gcgggggaaa gccgttttgc catcttgggt aaattggctg gcaagatgac tgtggcgatt
      601 tacacactcc ggggcgaagc aatcaggatc attagttgtc acagggcaga caaaaaactg
      661 gagagaatct atgaaaaact catcaaagaa aaacaaggtt aaaaaaatct ctgataccgt
      721 atttgacaaa atgtttgatg ctggggaaga catcagtgct tatctggaca caaccgcggc
      781 catcgttgtt caaagggtga acgttgattt tccgcaatgg atgatcaaac tactcgataa
      841 tcaggccaat caattaaatg tctcacgaca ggcagtgata aaaatgtgga tccgcgaccg
      901 attggagctg gtttatggca gacaagtttt tcacacacgg cccacagcct gattgactaa
      961 aagtgctgat tatctcacca cgggcgacac caagaactca cttccggtac tttcaccctc
     1021 accatcccgc ttgacaccac caaatactga gtcctgtatt aataagagcc tacaatggag
     1081 cagtagcacc ctgtcaaaac aaatctctca ggaccacctc tgaatcaacc atcgcttttt
     1141 tataggcatt atttttgact ccggacaggg taatgaatgt caacaaaaca tctttttcgg
     1201 ttttggtctt ttccttgaat acctgaattt tattttgaca gttttgggca tactccttgt
     1261 cgatcacaaa ctcatttttg gcacatttga tttcacaaag attaatcacc ccatcatcgc
     1321 ggtcaaataa aagatcaact tgcgcaccac catccttaag acactcacca ccaaagcatt
     1381 gccacggacc ggccacgtag gcggtatgat cagcactgat ggcacgcgca atctgagtca
     1441 ggtgtttgta acaaacgttc tcaaatgcat agcctgccca cgaggaccat tctggcgaag
     1501 tagatttttt taaccagtaa ttttcactgg cgatcacacc tttgcttagg ggatgaatcc
     1561 accgcgcgta acacaaagaa tactcatctg tgattctgta atggacgtcg tattgtttgc
     1621 catacggcac aaactttgtg atgaaaccag cctcctccaa ttcatcaaga cgcttttgcg
     1681 ccgtcccccc ggttttgagt ccaaccgccc tgaacaagtc ctcacgcgtg atcccgtaat
     1741 gatgttgtgc cagcttaaaa acaacctgcc tataaatagg caggcggcca aa
//
LOCUS       DATDUN010000508         3973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_149328, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000508 DATDUN010000000
VERSION     DATDUN010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149328"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VJC18_07020"
     CDS             <1..307
                     /locus_tag="VJC18_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45171.1"
                     /translation="VFIFMYKGRTATFAGLVNRLTSLIAGTVATLFVWLLMGGKFPKP
                     QDWVSLALMFVAIFFLSLSELKRTKELAATRELDEVANDVVVKESIPCKTSCCCGGR"
     gene            complement(329..3592)
                     /locus_tag="VJC18_07025"
     CDS             complement(329..3592)
                     /locus_tag="VJC18_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HLD45172.1"
                     /translation="MIKIIIEYSATHRFLVLLITSVFLIVGYWCLQNVPLDAIPDLSD
                     TQVIVYSRWDRSPDVIEDQVTYPIITALLGAPQVKDIRGFSDFGYSYIYIIFEDGTDP
                     YWARTRTLEYLSKITPRLPQGVQTELGPDATGVGWVYQYALVDKSGKHTIDELKSLQD
                     FNLKFQLQSVPGVAEVATIGGKSKQYQVVVDPNKLLAYNLALNTVVEQIRKSNNDVGG
                     RLVELNGREYMVRGRGYLKSIKDIELIHVGVDAKNGTPVLVKNIATVQIGPDIRRGVA
                     DLDGNGEVVGGVVIMRYGEDTPQVIARVKERLNEVATSLPDGVEIVPTYDRSELIEGA
                     IHTLKSTLIDEIIIVSLIILLFLWHIPSALVPIITIPASILIAFIPFYFMGLSANIMS
                     LAGIAISIGVLVDGAIVEVENAYKKLELWLHGGQKGDFHQVRLEALKEVGPSVFFSLL
                     VIAVAFIPVFTLVDQEGRLFKPLAYSKNLAMAIAALLAITLDPALRMLFARMEPMRMK
                     WGGLSRVLNTVFVGTYYPEEKHPISKRLFAIYEPVCRFVLNHKKKTMLAALGLMLITV
                     PVYFMLGSEFMPPLQEGVILYMPTTLPGLSVSEAERVLKKQDELLKSFPEVERVFGKI
                     GRAETPTDPAPYSMVETTIVLKPEAEWRHKERWYSFLPYFLEGPFRHFWSDRISYDEL
                     IGEMNQKVQMIGWTNAWTMPIKNRIDMLSTGIRTPVGIKVFGDDLSTIEKIGTELETI
                     LKDVKGTRSVYSERVAGGYFLDFDLKRESLARHGLSVDDANELIMTAIGGETMTTTIE
                     GRERYSVNVRYARDFRNDIDSLKRVLVSTPSGAHIPIGQLAEVHFTTGPAMIRNENGR
                     LSGYVYIDVSGHDVGGYASQAKKAVQANIKLPTGYTLRWSGQYENMQRVAERLKYVLP
                     LTLFIIFLLLYFNTKSFVKTGIVLLAVPFSLIGAIWFLWILGYNTSIAVWVGMIALMG
                     LDAETGVFMLLFLDLAYDSAQAKGQMQNKQDLYEAIVHGAVKRIRPKMMTVMAAFAGL
                     LPIMWSMQTGADMMKRIAAPMVGGLVSSFALELLVYPVVYAIWKERHLIKTTTPQTP"
     gene            complement(3589..>3973)
                     /locus_tag="VJC18_07030"
     CDS             complement(3589..>3973)
                     /locus_tag="VJC18_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45173.1"
                     /translation="MFTGVVKGLTPVVDASSRTLRARIYVSENDTSFKPNTYVNVSFA
                     IDLGEQLVVPKSALLDTGKHKLVYTVHDKTHFRPQEVQTGASWNEWQVINTGVKEGDT
                     VASAALFLLDSEAQLRMGKAGEHNH"
BASE COUNT         1013 a         1035 c          953 g          972 t
ORIGIN      
        1 cgtatttatc tttatgtata aaggccggac agccacgttc gccggtctgg tcaatcgcct
       61 cacgtcgctc attgcgggga ctgtggccac gctgtttgtg tggttgctca tggggggcaa
      121 gtttcccaaa ccacaggact gggtctctct ggctttgatg ttcgtggcca tcttcttctt
      181 gagcctttct gaactcaagc gcaccaagga gctggcggcc acacgcgaat tggatgaagt
      241 ggctaacgat gtcgtcgtca aagagagcat accctgcaag acatcatgtt gttgcggcgg
      301 gagatgagga acaggattga aaaatgattc aaggtgtttg tggcgttgtt gttttgatga
      361 gatgacgttc cttccaaatc gcgtacacaa cggggtaaac aagtagctcc aaggcaaacg
      421 aggaaacaag tcctcccacc atgggtgcgg caatgcgttt catcatgtca gcgccggttt
      481 gcatggacca catgatcggg agtaatcctg caaaggccgc catcacagtc atcattttgg
      541 gtctgatcct cttgacagct ccgtgcacaa tcgcctcata aagatcttgt ttgttttgca
      601 tttggccttt ggcctgggct gaatcgtaag ccaaatccaa aaacaacaac atgaacacac
      661 cggtttcggc gtcaagtccc atgagcgcga tcatccccac ccaaacagca atggatgtgt
      721 tgtaacccaa tatccacaga aaccagatgg ccccgatgag agaaaaggga actgctaaca
      781 gcacgatccc cgttttgacg aaagatttgg tgttgaagta cagcaaaaga aaaatgataa
      841 agagtgtgag tggcaacaca tatttgagcc gctcggccac gcgttgcatg ttttcgtact
      901 gcccgctcca acgcagggtg tatcccgtgg gaagcttgat gttggcttgc acggcctttt
      961 tggcctggct ggcgtaccct ccaacatcat gcccgctcac atcaatataa acataaccgg
     1021 aaaggcgacc gttttcgttt ctgatcatgg ccggccctgt ggtgaagtga acctctgcca
     1081 attggccaat ggggatgtgc gcccctgaag gggtggacac caaaactctc ttgagtgaat
     1141 caatgtcatt tctgaaatca cgcgcgtacc gcacatttac cgaatagcgt tctcgtcctt
     1201 caatggttgt ggtcattgtt tcgccaccta tggcagtcat gataagctca ttggcatcgt
     1261 ccacgctcaa tccatggcga gccaatgact cacgtttgag atcaaaatcc aaaaaataac
     1321 cgccagccac ccgttcgcta taaacgctcc tcgtcccctt gacgtctttg agaatcgttt
     1381 cgagctcggt gccgattttt tcgattgtgg acagatcgtc tccaaaaacc ttgatcccaa
     1441 ccggcgtgcg aattcccgtg gataacatgt caatcctgtt tttgatgggc atggtccacg
     1501 cgttggtcca gccgatcatc tgcacctttt gattcatctc gccgataagc tcatcgtaac
     1561 tgatccgatc gctccaaaaa tgtctgaaag gtccctcaag aaaatatggc aaaaaagaat
     1621 accatcgctc tttgtgtcgc cactctgctt ctggcttgag gacaattgtg gtttcgacca
     1681 tggaatacgg cgcgggatca gtgggagttt cggccctgcc aatttttcca aacacgcgct
     1741 caacttcggg aaaactcttg aggagctcat cctgcttttt aagaacgcgc tctgcctcac
     1801 tcacagacag acccggaagc gtggtgggca tgtaaaggat aaccccctcc tggaggggtg
     1861 gcatgaattc ggaacccagc atgaaataga ctgggacagt gatgagcatc agacccagag
     1921 cggcaagcat cgtctttttt ttgtgattca agacaaagcg acacacaggt tcgtaaatgg
     1981 caaacaatct tttgctgatg ggatgttttt cttcgggata atatgtgccc acaaaaactg
     2041 tgttcagaac acgcgataga cccccccatt tcattcgcat cggttccatt cgggcaaaga
     2101 gcatgcggag cgctggatcg agtgtgatgg ccaaaagcgc cgcgatggcc atggccagat
     2161 tctttgaata ggccaagggc ttgaacaacc gcccttcctg atccaccaag gtaaaaacag
     2221 gtatgaaagc aacagcgatg accaagagcg aaaaaaagac agacgggcca acctctttaa
     2281 gggcttccaa cctcacttga tggaaatcgc ctttttgacc tccatgcaac cacagctcca
     2341 attttttgta cgcgttctcc acctcaacaa tggcgccatc caccagcacg ccgatcgaaa
     2401 tggcaatgcc tgccagcgac atgatattgg cgctcagccc cataaagtaa aaaggaataa
     2461 acgctatcaa aatcgaagct ggtattgtga tgatgggaac cagggcagac gggatgtgcc
     2521 acaagaaaag gagaatgatg aggctcacaa taatgatttc atcaatcaac gtggacttga
     2581 gtgtgtggat agcgccctca atgagttctg aacgatcgta ggtgggaacg atctccacac
     2641 cgtctggaag tgacgtcgca acctcattca atcgttcttt gactcgggca atcacctggg
     2701 gtgtgtcttc accataacgc attatcacca cgccaccaac cacttcaccg tttccatcca
     2761 gatcggccac gccgcgcctg atatccggcc caatttgcac tgtcgcaatg tttttgacca
     2821 gcacgggtgt gccgttcttg gcatcaacac caacgtgaat gagttcgata tctttgatcg
     2881 acttgagata accgcgtcca cgcaccatgt attcgcggcc attcaattca acaagcctgc
     2941 cacccacatc gttgttgctc ttgcgaatct gttcaaccac cgtgttaagt gccaggttgt
     3001 acgccagaag tttattggga tccacgacaa cctgatattg ttttgacttg ccgccaatcg
     3061 tggcgacctc ggccacaccg ggcacgcttt gtaattggaa tttcagatta aaatcttgca
     3121 gacttttgag ctcatcgatc gtgtgtttgc cagatttatc aaccaaggcg tactgataca
     3181 cccatccaac tcccgtggcg tccggcccca actccgtttg caccccctgc ggcaaacgcg
     3241 gtgtgatctt tgaaaggtat tccaatgtgc gcgtcctggc ccagtaagga tcggtgccat
     3301 cctcaaaaat aatgtagata taagagtaac caaaatcaga aaaaccgcgg atgtctttga
     3361 cttggggcgc tcccaagagg gctgtgatga tgggataggt gacctgatct tcgatgacat
     3421 cgggactgcg atcccaacgg gaataaacga tgacctgtgt gtccgaaaga tcagggatgg
     3481 catcaagcgg gacattctgc agacaccaat aacccacaat cagaaacacg gacgtgatca
     3541 gcaaaaccag aaacctgtgc gtggcgctgt attctattat tatttttatc aatggttgtg
     3601 ctcccctgcc tttcccattc taagttgggc ttccgaatcc agtaaaaaca aggccgccga
     3661 ggcgactgtg tctccctctt tcacccccgt gttgatcacc tgccattcgt tccaagacgc
     3721 acccgtctgc acctcttgcg gtctaaaatg tgttttgtca tgaaccgtat aaacaagttt
     3781 gtgtttaccc gtatcaagca gggctgattt ggggaccacc aattgctcac ccagatcaat
     3841 tgcaaaggac acgttaacgt aggtgtttgg tttaaacgaa gtgtcatttt cggaaacata
     3901 aattcgcgca cggagtgttc gcgaactggc gtcgacaaca ggggtcaggc ctttaacaac
     3961 acccgtgaac att
//
LOCUS       DATDUN010000509         7955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_358292, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000509 DATDUN010000000
VERSION     DATDUN010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358292"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VJC18_07035"
     CDS             <1..181
                     /locus_tag="VJC18_07035"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526577.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HLD45174.1"
                     /translation="DSLKVVTGLVEPSLVGARVGEKFQFLRHGYFCVDEASAGEKIVF
                     NRTVSLKDSWAKVKE"
     gene            223..624
                     /locus_tag="VJC18_07040"
     CDS             223..624
                     /locus_tag="VJC18_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45175.1"
                     /translation="MERKKLFVAIALSLFVLPGAGHTAIKQKKRGYIFSGTIVLLLIL
                     LVLQVYFASKSTMMAEVLNSGSTDVFALAQTTSGNIMQELAGPIKVYGTIFMIIYFGS
                     VVDLIYLYKRANSPLGRWIEEQKREKNETRQ"
     gene            687..1025
                     /locus_tag="VJC18_07045"
     CDS             687..1025
                     /locus_tag="VJC18_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLD45176.1"
                     /translation="MKPNWTQEFSGAVTLSDATGKIVYMNQKASQVFEKQGGRKLVGK
                     NLMDCHSASSRKRLQEMIQKQEKNIYTIEKKGVKKLIYQAPWYHRGAFAGLVELSLEL
                     PAVMPHFIRG"
     gene            1059..1838
                     /locus_tag="VJC18_07050"
     CDS             1059..1838
                     /locus_tag="VJC18_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLD45177.1"
                     /translation="MKKSNFPNHQKFSSIQNIPLLGVGTIWFGRRWPAANQNWQMPSG
                     EEIQGYMSAAYECGVRMFDTAAAYGLSEKKLGEFFNNNPHILKESFVSTKWGEDFSLT
                     TEQSRIDHSAQNLIHSFDQSLTMLPRINLLYIHQATEAVLADARVKNKMQEYQRTGKI
                     KFTGASISDVTVLENVLERNLIWFDFMQTSAQVVFENPQLVQKIFDKGIAVVVNSPAR
                     KSATKTPEQAYKNLATLDCVSVILTGTRTHLKETAEYFEME"
     gene            1841..3211
                     /locus_tag="VJC18_07055"
     CDS             1841..3211
                     /locus_tag="VJC18_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45178.1"
                     /translation="MKNIKPESFVPDRWFCMSLKRFGSKLCLEIGGKIIEVQQLFDSI
                     KSQVPQCFRGGIVFSTTIFKNNIQLFDSLVSKQFSQRQIYWALKSAPIQMFAEVAAKQ
                     NIGIEVASHEELTMALNAGVPGSSICHSAAAKYDWDIDAIVKNDCVSISDNLVELALI
                     NDKSKALGCKKTKIALRINPAIASQTSSLISTGTATCKFGIPDISNDLMQKIKTFSHL
                     DIMAVHMHIGSQIADPDNYCQALKNLIRAYRLLLKHGFVIHAIDVGGGFPYSYRISDD
                     GQNVDDHGHHANLLRYEYDEYLVRIHKTMVDELGPDLPTIAFEPGRLLTAGSAVTLGY
                     VLHTKEYPNNLRWALVSISVNDLWVKEITPEIYFNPLMVRECSQETLPTAIGGTLCYS
                     GDIITPPDTTIAMPIDMQRDDVVVFEHTGAYTLLGAGTFHNMPRLPVFVLNDDLELVR
                     EVLPQV"
     gene            3303..3560
                     /locus_tag="VJC18_07060"
     CDS             3303..3560
                     /locus_tag="VJC18_07060"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HLD45179.1"
                     /translation="MNFSITAFPETKIVGVALRTDNSPACLQKLGAHWQRFFAENILE
                     KIPNKTSHEIYAVYTDYDSDRPVGSEGLLGRGRKKCNPCRR"
     gene            3704..5038
                     /locus_tag="VJC18_07065"
     CDS             3704..5038
                     /locus_tag="VJC18_07065"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45180.1"
                     /translation="MLNAQEKIELIVREGEGQTIEFKQNVSNLDKGMVAFANAMGGSV
                     FVGINDQGVVTGIKSTNRLVSQIQDIARNCDPPIKIKIEKLDSVLEVVVAESANKPHR
                     CQSGFYLRVGPNSQKLNRDEIIQFAVGEGRVRFDEQINSRFNFSEDFSPEHYEMFSRL
                     AKWPASISSQDALINLAVAQKQKKEVLLTNAGVLFFATNPQKFFPEAYITCVRYRGSD
                     RFSIIDRKDMKGPPITQLEEAMNFFKRHTEEAVVVSGNLAHQVRVEYPTVAVREALVN
                     AATHRDYLYDGSHIYMHLFSDRMEIENPGGLFKGLTLEDLGKRSVRRNRLIADLFFRA
                     GYIEMIGSGITRISQALSANGNPSPEITATNFFNMTFKKSLGLESKVSLTNRQKQLYL
                     FMWGQQGAVTRDACAQFLGVSGDTALREIKELMKLNLIEQTGRGKGVRYRRK"
     gene            5069..5260
                     /locus_tag="VJC18_07070"
                     /pseudo
     CDS             5069..5260
                     /locus_tag="VJC18_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455161.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
     gene            5782..6030
                     /locus_tag="VJC18_07075"
     CDS             5782..6030
                     /locus_tag="VJC18_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45181.1"
                     /translation="MSSKRLQVIMDEAEYGSFQKLAECVQMSLGEWVRMAMRHFAKNY
                     STTNEDQKILALRHAATQKFPSGDIDDILSQINSGYTS"
     gene            6027..6443
                     /locus_tag="VJC18_07080"
     CDS             6027..6443
                     /locus_tag="VJC18_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLD45182.1"
                     /translation="MIIFIDSNIPMYVIGQDHPNKQTSILLLERFIGEKHRLVTSTEV
                     LQEILHRYKAIDRTAAIQPAFDCLYGIVDEVLPVTEADVLEAKKNVLAYKGLSARDAL
                     HVAHVKNHDLSAIMSFDGGFDQVPGVTRIWDLSARF"
     gene            complement(6458..>7955)
                     /locus_tag="VJC18_07085"
     CDS             complement(6458..>7955)
                     /locus_tag="VJC18_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45183.1"
                     /translation="FEQTRIVFRSLLNHPGAVVVNTLRASSKIKRYAAISSYAGAEDT
                     HGCRFAIRGPEGPVLDSVLIDWGIKGYRENVITFKNTKDLPLGSLLCPNPYETPHEQN
                     QIAGWGLSNQIVCSALLQLFEAPPIKDMLGMLCLDSWSDDFDNDAGVTITAQPEDIET
                     GFVCGRGPRLTKTHPRHISIPCRHHHNGASHGEWRLELCNKTDVVELSHRLISLALDS
                     QPQQKTHKPLHSPQIEIHDHVSSVLAVGEPPPSYTEAITTTLKKWNEIHAQQELHPLA
                     VSAHHWPALRNSLISCGCIVRDIKQDVPSVLDAIITYCPLFKGSKTMRVHFFAHAQAE
                     DLFRPHHTLFSVESFSKLNWSSFMAFKLASNLCESRLETFASRPILKHLVSNGFGLWL
                     ACQLFNCQSADVLGVELSVYETLERNILEWRSRLSDFVTGAFWEIKQGRHEELRLKEI
                     SNPFSGEGGVPHYGNFLGYLFVRDYLKTNGLSSLMSEEGLLAAETYFS"
BASE COUNT         2207 a         1689 c         1976 g         2083 t
ORIGIN      
        1 ggattcgctc aaggtggtga cagggctggt ggagccttct ttggtggggg cgcgggtggg
       61 tgaaaaattc cagttcctgc ggcacggtta tttttgtgtc gatgaggctt cagccggcga
      121 gaaaatagtg tttaatcgca cggtctctct taaagacagt tgggcaaagg tgaaggaatg
      181 aaacaatcaa gcaatgaaac aatgaaacaa tggtggattg ttatggaaag aaaaaaattg
      241 ttcgtagcca ttgccctcag tttgtttgtc ttgcccggag caggacacac agcgatcaaa
      301 caaaagaaac gaggctacat cttcagcggc accattgtct tgttgctgat tctgctggta
      361 ttgcaggtat attttgcttc gaagagcacc atgatggccg aggttttgaa ctccggtagc
      421 accgatgttt tcgcactggc tcaaacgacg tctggtaaca ttatgcagga gttggcgggc
      481 cccattaaag tgtacggaac tatttttatg atcatctatt ttggctctgt ggtcgatctc
      541 atctatttat acaagcgcgc caatagtcct ttgggacggt ggatcgagga gcagaagagg
      601 gaaaaaaatg agacacgtca ataaagcagt gacagatctc gtgatgttca tgacacatac
      661 ggcgggttcc aaataagatg aaacaaatga aaccaaattg gacacaggaa ttctctggtg
      721 ctgtgacgct gagtgatgcc acaggcaaaa tagtttacat gaaccaaaaa gccagtcagg
      781 tttttgagaa gcaaggtggt cgcaagctgg tgggcaaaaa tctgatggac tgccattctg
      841 cgagttcgcg caagaggctt caggaaatga tccagaaaca agaaaaaaac atttacacaa
      901 tagaaaaaaa gggcgtgaaa aaactgattt atcaggcgcc ttggtatcac aggggcgcct
      961 ttgctgggct ggtggagctg tcgcttgagc ttccggctgt catgccgcat tttatacggg
     1021 ggtagcgagt gatggcgatt gattaaaagg atgagtcaat gaaaaaatct aattttccaa
     1081 atcatcaaaa attttcatca attcaaaata tccccctgtt gggcgtagga acaatttggt
     1141 ttggacggcg atggccagcg gcaaatcaaa attggcaaat gccgagcgga gaggaaattc
     1201 aaggttacat gagcgccgct tacgagtgtg gtgtgcgcat gtttgataca gcggcggcct
     1261 atgggttgtc tgaaaaaaag ctgggagaat tttttaataa caatccacat attctcaagg
     1321 aatcatttgt ttcaacaaag tggggagaag attttagtct tacaactgag caatcacgga
     1381 ttgatcattc tgctcaaaac ctcattcact cttttgatca gagcctaaca atgttaccac
     1441 gcataaactt gctgtatatt catcaagcaa ccgaggctgt tttggcagat gcgcgagtta
     1501 aaaacaaaat gcaggaatac cagagaacag gaaaaatcaa atttacgggg gcatcaatat
     1561 cagatgtgac agtgctggaa aatgtgctgg agcgcaactt gatatggttt gactttatgc
     1621 aaacaagtgc ccaagtagtt tttgagaacc cccaacttgt tcaaaaaatc ttcgataaag
     1681 gaattgccgt tgtggtgaac agccccgctc gaaaatcagc aaccaaaaca ccagaacaag
     1741 catacaagaa cctggccacg ctggattgtg tttcggttat tctcacggga accagaacgc
     1801 acctaaaaga gaccgcggaa tattttgaaa tggagtaata atgaaaaaca tcaaaccaga
     1861 atcctttgta cctgaccgct ggttttgcat gtccctcaaa agatttggtt caaaattgtg
     1921 tttggaaatt ggtggcaaga taattgaggt tcagcaactt tttgattcta tcaaaagcca
     1981 agtcccacaa tgttttcgtg ggggcattgt tttctcaacc acaatattta aaaacaacat
     2041 tcagcttttt gattctctcg tttcaaaaca atttagtcag cgacaaatct attgggcgct
     2101 taaatctgca cccatccaaa tgtttgccga ggtggcggct aaacaaaaca ttgggataga
     2161 agtggcctca cacgaagaac tcacgatggc cttgaatgct ggcgtgcctg gttcctcaat
     2221 ctgtcacagt gccgccgcca agtatgattg ggatatcgat gccattgtaa aaaatgattg
     2281 tgtctcaatt tctgacaatt tggtcgaatt agctttgatc aatgacaagt caaaggccct
     2341 tggctgcaaa aaaaccaaaa tcgcactccg aatcaatccc gccattgctt cacaaacatc
     2401 cagcctgatt tcgacaggga ctgcaacgtg caagtttgga atccctgata tttccaatga
     2461 tctgatgcaa aaaatcaaga cgttttcgca tcttgatatt atggctgtgc acatgcacat
     2521 tggttcgcag attgcagacc ccgataacta ttgtcaggca ttgaaaaacc tgattcgggc
     2581 ctaccggtta cttcttaaac acggctttgt cattcatgcc atcgatgtgg gcggtggttt
     2641 tccttactcg tatcgaattt cagatgatgg ccagaatgtg gatgatcacg ggcatcacgc
     2701 gaatttgttg cgttatgaat acgacgagta cctggtacgc attcacaaaa caatggtgga
     2761 tgaacttggg ccagaccttc caacaattgc ctttgagccg ggccgacttt tgaccgcagg
     2821 gagtgcggtg accttgggtt atgtgctcca taccaaagaa tatcccaaca atttgagatg
     2881 ggcattggtt tcgatttcgg tcaacgatct gtgggtcaag gaaatcacgc cagaaattta
     2941 ttttaatccg ctgatggttc gcgagtgttc acaagagaca ctgcctacag ccatcggagg
     3001 caccttgtgt tattccgggg acattatcac tcctcccgac accacgatcg ccatgccgat
     3061 tgacatgcag cgggacgacg ttgttgtttt tgaacacacg ggtgcctaca cgcttttggg
     3121 agcaggcacg tttcacaata tgccgcgctt gccggtgttt gttcttaatg acgatcttga
     3181 gttggttcgt gaggtgttgc cgcaagtttg aggaaagggc agggcagatc ggtttattat
     3241 gacatgaaaa aaccaaattc tcgcgacact gtagcgagaa ttgtgccgaa agaacaaata
     3301 taatgaattt ttcaatcaca gcttttccag aaacaaaaat cgttggtgtt gctctccgca
     3361 ctgacaacag cccagcatgc ttgcaaaaac ttggtgcaca ctggcagcgg ttttttgccg
     3421 agaacatttt agaaaaaatt cccaacaaga ccagccatga aatttatgca gtctacacgg
     3481 attatgatag cgacagacca gtcgggagtg aaggtttgtt ggggaggggg cgtaaaaagt
     3541 gcaacccatg caggcggtaa acgccaatta aatcaacgtt gtaaggcttg ttgtttgtcg
     3601 ctgatcatgt tttgcgtcgc atttgcgtcg catttgcgtc gcaaataatt gacatgtttg
     3661 gattgttttt ggtagcgaga gctccaaact ggaggagcca taaatgttga acgcccaaga
     3721 aaagattgaa ctcattgtca gggagggcga aggacagacc atcgagttta aacaaaatgt
     3781 ttcgaatctg gataaaggaa tggtggcctt tgccaacgca atggggggtt ctgtttttgt
     3841 tggtatcaat gatcagggcg ttgtgaccgg gattaaatcg accaatcggt tggtcagcca
     3901 aattcaagac attgcccgaa attgtgatcc gcccattaaa ataaaaatcg aaaagctgga
     3961 cagtgttctc gaagttgtcg ttgccgaaag tgctaacaaa ccgcatcggt gccagagtgg
     4021 attttatttg cgcgttggcc ccaattcaca aaaactgaac cgtgacgaaa tcattcagtt
     4081 tgctgttgga gaaggcaggg ttcgttttga tgaacaaatc aattctcgct ttaattttag
     4141 cgaagatttt tctcctgaac attatgaaat gttttctcga ttggctaagt ggcccgcctc
     4201 aatttcatcg caagatgcgc tcatcaacct tgctgtagcc cagaaacaaa agaaagaagt
     4261 cttgctcacc aatgccggcg tcttgttttt tgcaacgaat cctcaaaagt tttttccaga
     4321 ggcttacatc acctgtgtgc gttatcgcgg ttcggatcgt ttttctatta ttgacagaaa
     4381 agacatgaag gggccgccca tcacacaact tgaagaggct atgaactttt tcaagcgaca
     4441 cacggaagag gctgtcgttg tttccggcaa tcttgcccac caggtgcgcg tagaatatcc
     4501 cacggtggct gtgcgcgaag ctctggtcaa tgcagccacg catcgcgatt atttgtacga
     4561 cggttctcac atctacatgc atcttttcag tgacaggatg gaaattgaaa atccaggagg
     4621 tttatttaag ggcctcacgc ttgaagatct ggggaaacga agtgtgcgaa gaaacaggct
     4681 cattgccgat ttgtttttca gagcggggta tattgaaatg attggaagtg gaataacgcg
     4741 gatctctcag gctctatctg ccaacggtaa tccctcgcca gaaatcacgg ccacaaattt
     4801 ttttaatatg actttcaaaa aatcgttggg tttagaaagc aaagtgtcgc tgaccaatcg
     4861 acaaaagcaa ctctacctct ttatgtgggg ccaacaaggc gctgttacga gggatgcctg
     4921 tgcgcagttt cttggcgtga gcggggatac ggcattgaga gagatcaaag aactcatgaa
     4981 actcaatttg attgaacaga cgggcagggg gaaaggtgtg cggtataggc ggaagtaaaa
     5041 acttatttgg cggtgtagga ggaaaatgat gatcacactc actgtacact caggtcttga
     5101 tgcggtcggg ttcttggcgc ggatcagcac ggctcttgcc gaagctggca tcagtctcaa
     5161 cgctgtctct gcattttatc atgatcatct ttttgttcct gtcgacaggg caggggaggc
     5221 gatggtggcg ttggagagat tgtctcatta tgactcctag gcttttaaac tgcgtaaaga
     5281 ttctcatttg gattgctcca tctgctgagc ccgctatgcg gttatcaaat cgtcatcagc
     5341 acttttttat ctggcatgct gctgaattac gcgtacttct attcaggaaa tttatatttg
     5401 ccgatcggaa ttcactttgg gtggaatttt tttaatagtc ttttgttttc agtaagaatt
     5461 attaaagttg agtatctaaa caattttttg gcaggcgcaa aaaatccgga acagggcttg
     5521 atcgcaatct tcgtaacggg ttctgtcgca atcggtttaa tgctattgta ttttcaaaga
     5581 caaagtgttg gaaaataaat aacatcagaa cttactaacc ccccatctct cttttattct
     5641 tattgacccg ttatcggcag taaggacggt caagattccc agccaacggc gcaacagaca
     5701 gaaaacgaac cgcaccaagc gatcgaaata actatgctta ttgttgttgt gtcatgccta
     5761 tgagtatgat atactcatca tatgagtagc aagcggttac aggtcattat ggacgaggct
     5821 gaatacggtt cgtttcaaaa actggcggag tgtgtccaaa tgagtttggg tgagtgggtg
     5881 cgcatggcca tgaggcactt cgccaaaaac tattccacaa caaatgagga tcaaaaaata
     5941 ttggcgcttc gtcatgcggc gacccaaaag tttcctagcg gtgacattga cgacatcctt
     6001 tcgcagatca attctggcta tacttcatga tcatctttat tgattccaac attcccatgt
     6061 acgtgattgg tcaggaccat cccaacaaac aaacctcgat tttgttgctt gaacgcttca
     6121 ttggcgaaaa acatcgtttg gtgaccagca cagaagtttt gcaagaaata ttacatcggt
     6181 acaaggccat tgacagaacg gcggcaatcc agccagcgtt tgattgtttg tatgggattg
     6241 ttgacgaggt tttgcctgtg actgaggccg atgtgctcga agcaaaaaaa aatgtgttgg
     6301 cctacaaggg gctttcggca cgcgacgcct tgcacgtagc acacgtcaaa aatcatgacc
     6361 tgtccgcgat catgtccttt gacggcgggt ttgaccaggt tcccggggta acaagaattt
     6421 gggatttgtc cgccaggttt tgacgggaac taacgaatca actaaaatat gtttccgcgg
     6481 ccagcaagcc ctcctccgac atgagcgaag acaggccgtt ggttttgagg taatcgcgaa
     6541 caaaaagata tcccaaaaaa tttccgtaat gtggcacccc cccttcgcca gaaaaagggt
     6601 tgctgatttc cttgagacgc aactcctcgt gtcgtccctg tttgatttcc cagaacgcgc
     6661 cagtcacgaa atcgctgagc cgggagcgcc attcaaggat gtttcgttcc agggtttcat
     6721 aaacagaaag ctccacaccc agcacgtccg cggattggca gttaaaaagt tgacacgcta
     6781 accagagccc aaaaccgttt gagacgaggt gtttcagaat ggggcgactg gcaaaagttt
     6841 ccaggcgtga ttcacacaaa ttggacgcca gcttgaaggc cataaaagag gaccaattta
     6901 attttgaaaa agactcaaca gaaaacaagg tatgatgtgg tctgaaaaga tcctcagcct
     6961 gagcgtgcgc aaaaaaatga acgcgcattg tttttgagcc tttgaaaaga gggcaatagg
     7021 taatgatggc atcgaggaca gagggcacat cttgtttgat gtctctgaca atgcacccgc
     7081 atgaaatgag gctgtttcgc agagcaggcc aatgatgagc agaaacagcc aggggatgca
     7141 attcctgttg ggcgtgaatt tcgttccatt ttttgagagt cgtggtgata gcctcggtgt
     7201 acgaaggagg tggttcaccc acagccagca cggacgagac gtggtcgtga atttcgattt
     7261 gtggtgagtg aagcggtttg tgtgtttttt gttgtggttg cgaatccagg gccagcgaaa
     7321 tcaacctgtg tgatagctcg acgacatctg tcttgttaca cagctccagc cgccactcac
     7381 catgagaggc cccgttgtga tggtgccgac aggggatgct gatatgacgg ggatgagtct
     7441 ttgtcagacg aggccccctg ccgcagacaa aacctgtctc aatgtcttcg ggttgtgctg
     7501 tgatggtgac ccccgcgtca ttgtcaaaat cgtcggacca ggaatccaaa caaagcatgc
     7561 cgagcatgtc tttgatcgga ggggcctcaa atagttgcaa cagtgccgag cacacaattt
     7621 gatttgagag accccacccg gcaatttgat tctgttcatg tggcgtttcg tagggatttg
     7681 gacagagcaa agaacccagc gggaggtctt ttgtgttttt gaaagtgatg acattttcgc
     7741 ggtatccctt gatgccccag tcaatcaaca cggaatccaa cacaggcccc tcgggaccac
     7801 ggatagcaaa acggcaaccg tgagtgtcct ctgccccagc ataagatgaa atggcggcat
     7861 atctttttat ttttgaagac gcgcgcaatg tgttcacaac aacggctccg ggatggttga
     7921 gtagagagcg aaagacaatt cgggtttgct caaat
//
LOCUS       DATDUN010000510         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_66367, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000510 DATDUN010000000
VERSION     DATDUN010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66367"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1161
                     /locus_tag="VJC18_07090"
     CDS             211..1161
                     /locus_tag="VJC18_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45184.1"
                     /translation="MNTLDQQEALTRVSSAHTSHSTYLDCWRGTISAQTFVDLSAPMV
                     EDFHLYEMSENPNILMMDFRDSERQSRYLDRISYFVEIEPDDLMDELDFITLLDTKGV
                     HCEEDEKDRVCGLGLGHDLKLTDVARFFNTIAQNGLELTLEENQLLDSLQMLGLLDVH
                     TDGFAEATDNAVIISTFRDFSPLYRHDVMAHEIQHGHYFTNSAYRSDVNAVWDRLQPN
                     VHKTLASILFYSGAHYNIDDDVLVRNEAASFSVETQTENNGWFGLFASAQLHIDSAMP
                     HPDIIPPSEFKSLSVSDYATLFAQLHSVYTLIEDCHDVYR"
     gene            1782..>2496
                     /locus_tag="VJC18_07095"
     CDS             1782..>2496
                     /locus_tag="VJC18_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLD45185.1"
                     /translation="MLQLRPNITQLPINGFAIEFVDAFPLNVPLKDPFVVASTRLDTV
                     QNVAVRVGIGNPSSRAVGWGEIPTLHPITPETQPIAMKAVAQGAQWLVGQNPKPWFDL
                     ADGLKEQIPEFPAVRAGIEMAIFDAALRAHQMPVAAFFDGGLYEGWTHTSSHFIRTDI
                     TVPICTSKQATELAYRYAANGFTHFKVKVGNNILNDVARIVAIRKVYPACTLFIDANE
                     GYDVNAALTLLNRLKSHGIF"
BASE COUNT          654 a          641 c          533 g          668 t
ORIGIN      
        1 gcagtaccgc aaagagcacg gctgtccagt acccaaagtg cttcaaaaac aaatactcgg
       61 gctcagagac cgctttggcc tcaactacaa attcagtttt gattactgag cgctgtagct
      121 atccccctga tttacttgcc cttattattt tcgatttttt acacaacctg tgccaagacc
      181 agccgataag gattgcgggt tacacacaca atgaacaccc ttgatcaaca agaggccctc
      241 acccgggtat caagcgcaca taccagtcac tcaacctacc ttgactgctg gagaggcacc
      301 atttctgcac agacatttgt ggatctatct gcccccatgg ttgaggattt tcatctctat
      361 gagatgagcg aaaaccccaa tatcctgatg atggatttca gagatagcga aagacaatca
      421 cgctatctcg atcgcatttc ctattttgtg gaaattgagc cggacgatct gatggacgaa
      481 ctggatttta ttaccctgct cgacaccaag ggtgtgcatt gcgaagaaga cgaaaaggac
      541 cgtgtgtgcg gtttggggct cggacacgac ctgaaattga cggacgtcgc cagatttttt
      601 aacaccattg cgcaaaatgg ccttgaactc acccttgagg aaaatcaact gctggatagc
      661 ttgcaaatgc tggggctcct tgatgtgcac acagacggat ttgcagaggc caccgataat
      721 gcggtgatca tctcgacatt tcgtgatttt agtccgctct acagacacga tgtcatggcg
      781 catgagatcc agcacggaca ctattttacc aattccgcct accgcagtga tgtgaacgct
      841 gtttgggatc ggctccagcc caatgtgcat aaaacactcg cgtccattct tttttattcg
      901 ggtgcccatt acaatattga cgatgacgtg ctggtcagaa acgaagccgc ctccttcagc
      961 gtcgaaacac agacagaaaa caatggctgg ttcggactgt tcgcttccgc tcaactgcat
     1021 atcgattcag ccatgccgca tcccgatatc atccccccct ctgaattcaa atccctttcc
     1081 gtctcagact acgcaaccct gtttgcccaa ctgcattcgg tctacacgct cattgaggat
     1141 tgtcatgatg tgtatcggta gtgagtcagc cgttggtgat gttttttccc ttgaatagcg
     1201 acagggtaac ccaacttgcg cagttagcgt agtgttcatg ttgttattga acgaacccac
     1261 ctccagccga aggactgatc cgtcctctgg cggacgtgtg agctttggtg gaagctcgaa
     1321 cactccacgg tttatccaat tcttacctgt tatcggttag gtcttagatt tgttgtctca
     1381 gttggtacga tttcgtactc tgaagagtac gggacaggaa aattcctagc ctcaaataca
     1441 aacaactatt tcgctgtatt gattgtgtcc gttgggctgt gatatcctct tggaaaagga
     1501 tagctatgtc cataaacaat gaaaactctc tactcaaaaa taaattcaaa gaccacatga
     1561 ttttgaaagg attttctcca gaaacccaaa gaacctacat ttcatccttg agtggtttgt
     1621 accgatttta caaatgaaca aattcaacat tctgtctgca cagacggagt cagatcattt
     1681 ccaaactcgc gatcctcaac aatttcgatc gcgatgatgt tgaccaactc aaaaaaattt
     1741 gcattccgtg catcccctgc gatattgcca ccaacactgc tatgctacaa ctcagaccca
     1801 acatcacgca acttcccatc aacggttttg ccattgaatt tgttgatgcc ttcccactca
     1861 acgttccact caaagatccg ttcgtcgttg cctcaaccag attagatacc gttcaaaatg
     1921 tggctgttcg tgtgggtatt ggcaatccat caagcagagc tgttggctgg ggcgaaattc
     1981 caacgcttca ccccatcaca cccgaaacac aacccatcgc catgaaagct gtcgcccaag
     2041 gggctcagtg gctggtgggt caaaatccaa aaccttggtt tgatttggct gatggattaa
     2101 aagaacaaat tccagaattc cccgctgtgc gcgcagggat tgaaatggcc attttcgatg
     2161 ccgcacttcg tgctcaccaa atgcccgtcg ccgctttttt tgacggtggt ttgtatgagg
     2221 gatggacaca cacttcatcg cacttcatca gaactgatat tactgtcccc atttgcacaa
     2281 gcaagcaagc cactgaactt gcctatcgtt acgctgccaa tggatttacg catttcaaag
     2341 tcaaagtggg caataacatt cttaatgatg tggcccgtat tgtggccatt cgtaaagttt
     2401 atcctgcttg caccctattc attgatgcca atgagggtta cgatgtgaac gcggcgttga
     2461 cactgttaaa cagactcaaa agtcacggca ttttcc
//
LOCUS       DATDUN010000511         2435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_440589, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000511 DATDUN010000000
VERSION     DATDUN010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440589"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..747)
                     /locus_tag="VJC18_07100"
     CDS             complement(187..747)
                     /locus_tag="VJC18_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45186.1"
                     /translation="MYNQQIGSGYIATSVITAPQLATTQNDATVQQVLSASMIEDYDT
                     QLSDISNQMKSELNEKQNIRKEIEEIQSYSALPSTTVQEGVDCVELTVEQAQKINVTE
                     TAMPIKDSGGNVIGYYVDKAQLTDSVKNAVEVRKERLETLNSQSEMTMINVQQLVAKR
                     NQALMLLSNLYASQNDCMKQIIGNFK"
     gene            complement(769..1062)
                     /locus_tag="VJC18_07105"
     CDS             complement(769..1062)
                     /locus_tag="VJC18_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45187.1"
                     /translation="MNTGQQQNHTDDRIEQVLKQYQKEQKEFENHWLWKELGTTPKDF
                     FAKINEYVAKSGIDKKVWEQKLAEAKMAVETKLREKQGSYKSVNLGALRGIRV"
     gene            complement(1342..>2435)
                     /locus_tag="VJC18_07110"
     CDS             complement(1342..>2435)
                     /locus_tag="VJC18_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HLD45188.1"
                     /translation="EESDEASLGYAVPLLLELSPKLYNDFLHDDRWRRCFNHLYPRLR
                     QELTQRLGIPLPELKIAVNEELLQDRYVIKIYEIPVDEGFLSPEHCLVQKNDQLPNWD
                     TLMSEHEAKETNTVHGSKVMLIENRHQQNLGLNGIRAFSPEEVLLKHIARVVVKQASD
                     FVGVQEVKDVLSHVERRHPDLVREVIPRMLTVQKLTEVIKRLVDEGVSVKDFRLLLET
                     LTVVRPEDKSAVDLTELVRMGLKRQITHRYTNRSGMLSCFGLDPEIEEEVAKHIKKTG
                     QDCFLTLPPNRIEAITRAARLAYRSHRASPKKVVVLTNPEIRRYVRKMLELELPDVSV
                     LSYQEIEPTILIDQKDTISLNYPEMVMSA"
BASE COUNT          559 a          628 c          530 g          718 t
ORIGIN      
        1 atctggctgg ccatgccttt cagttggtcg tcatattcct gcaccaacaa catctgtaca
       61 gatgtcatga gatcaactcc gttcatgacc gatgaatcgg ttattccagt ggctttgatt
      121 tcggttggca tgacattgct aaacatatta actccttttg cctttgccaa gacaaaggct
      181 atttgattat ttaaaattac caataatctg tttcatgcaa tcgttctgac tcgcatacaa
      241 attggacaac aacatgagag cctgatttct cttggccact aattgttgaa cattgatcat
      301 ggtcatttcg gattggctgt tcagggtctc caagcgctct ttgcgtacct caacggcatt
      361 ttttacagaa tccgtaagct gtgctttatc aacataataa cctatcacat tgccaccgct
      421 atccttgatg ggcatggctg tttctgtcac attaattttc tgcgcctgtt cgacagtcaa
      481 ttccacgcaa tcaacgcctt cttgtaccgt tgttgatggc aacgccgagt atgactggat
      541 ttcctcgatc tctttgcgaa tgttttgttt ttcgtttaat tcggatttca tttgattgga
      601 aatatcggaa agttgcgtat cgtaatcttc tatcatggag gcactcagca cctgctgaac
      661 tgtcgcgtca ttctgcgttg tagccaattg tggtgccgtt atgactgacg ttgcaatgta
      721 acctgagcct atttgttgat tatacatatc gacctcttct aaaaaatgtt acactcgaat
      781 tccccttaaa gcgccaaggt tcacagattt ataggaaccc tgtttttcac gaagttttgt
      841 ttcgaccgcc atcttcgcct cggccagttt ttgttcccac acctttttat cgatcccact
      901 cttggcgaca tattcgttga tcttggcaaa gaaatccttg ggcgtggtac caagctcttt
      961 ccacaaccag tgattctcaa attccttctg ttccttttga tactgcttga gcacttgctc
     1021 aatgcgatcg tctgtgtggt tttgttgttg ccctgtattc atgccttagg gatattcaag
     1081 gatcgtgcca aaacacggaa aataattttg ccagtcttat tcgtttgtta ctgattttgt
     1141 cttgaatgag actgtctcaa atctcacaca ccgtcccagc tgtgggacgg tttttttgtc
     1201 gtggtgggac ggggggccta aggaacgaat ggacgacagc caacggccaa acatttttct
     1261 ctgagcgtca gccgcagaca attgaccgtt agccttcccc tttgagcttc ttgccctgag
     1321 cttgtcgaag ggttgaaccc attacgcact cattaccatc tcgggatagt tgagagaaat
     1381 ggtatctttt tgatcgatga ggatcgtggg ttcgatctcc tgatacgaaa ggaccgaaac
     1441 atcgggcaat tcgagctcca gcatcttgcg cacataacgc ctgatctcgg gattggttaa
     1501 aaccacaacc ttcttgggcg aagcccggtg actacggtac gccagccggg cagcccgtgt
     1561 gatcgcctcg attcggttgg gaggcagggt caaaaaacaa tcctggcctg tttttttgat
     1621 gtgtttggcc acttcttcct cgatctcggg atcgagccca aaacacgaca acatgcccga
     1681 gcggttggtg taacgatgcg tgatctgccg tttgagcccc atccgcacca gttcggtcaa
     1741 atcaacagcg ctcttatcct cgggacgaac gactgtgagt gtttccaaaa gcagtctaaa
     1801 atccttgacc gaaacccctt catcgaccag cctcttgata acctctgtga gcttttgcac
     1861 ggtcaacatg cgcgggatca cctctcgcac caaatcggga tgtcgacgct caacatgaga
     1921 cagcacatcc ttcacctcct gcacccccac aaaatcactc gcctgtttca ccacaacgcg
     1981 ggcaatgtgt ttgagcagga cttcctcggg agaaaacgcg cgaatgccat tgagtcccaa
     2041 attctgttga tgcctgtttt caatcaacat caccttcgat ccgtgcacgg tgttggtttc
     2101 cttcgcctcg tgttcactca tcagcgtgtc ccagttcggc agttgatcgt ttttttgcac
     2161 cagacagtgt tcgggagaca ggaatccctc gtccacaggt atttcgtaaa ttttgatgac
     2221 atagcgatcc tgcaacaact cctcattcac cgcaattttc agctccggca agggaatccc
     2281 cagtctttgc gtgagttcct gccgcaaccg ggggtataag tggttaaaac agcgtctcca
     2341 ccggtcatcg tgtaaaaaat cgttgtacaa tttgggactg agttccaaca gcagtggaac
     2401 agcgtaaccc aatgacgcct catcgctctc ttcgc
//
LOCUS       DATDUN010000512         1489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_27685, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000512 DATDUN010000000
VERSION     DATDUN010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27685"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VJC18_07115"
     CDS             <1..273
                     /locus_tag="VJC18_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLD45189.1"
                     /translation="VPVMQGNAGYINQHGDLQVTIEGHCDNRGSNEYNLALGHRRAQS
                     AANYLMNLGVDGGRLRIVSFGEERPVCQYDNEDCWQRNRRADFRKQ"
     gene            374..1162
                     /gene="ybgF"
                     /locus_tag="VJC18_07120"
     CDS             374..1162
                     /gene="ybgF"
                     /locus_tag="VJC18_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HLD45190.1"
                     /translation="MKNKIIPAFLLVVLFSTSLVYARKNDDSAYAPQDYDTMRTAIAN
                     QGTSMANMQSQISQITGSLQTLTGDVNRTYKENRDQEKTIADAHNRMQSLEDKMTILT
                     TQLAELQTEGFLKPQASKRLSEYKEYATGLEFVNAAQYDKAVQAMQSFQSKNPKSIYK
                     SFAQFWIGEAYYQQADYQMAIKEFQKLFELNPQSAKAPTALYKQGLSFAQLKSYEDAK
                     AFFANVIRTYPASLEAIQASSQLRRIENVSELNRQQNRELNLVQ"
     gene            1192..>1489
                     /locus_tag="VJC18_07125"
     CDS             1192..>1489
                     /locus_tag="VJC18_07125"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD45191.1"
                     /translation="MNVLQRLAFSPLFTQLVITRRCNLSCSYCNEFDRTSTPVPTETL
                     KQRICQIKDLGSFSICLTGGEPFLHPDLCGLLSFCRHEQKFFRTSMITNGYFLTP"
BASE COUNT          414 a          423 c          323 g          329 t
ORIGIN      
        1 gtccccgtca tgcagggaaa tgccggttac atcaaccagc atggcgacct ccaggtcacc
       61 attgagggtc attgcgacaa ccgtggttca aacgaataca acctggccct aggacatcgc
      121 cgcgcccagt ctgccgccaa ctacctcatg aatctggggg ttgacggtgg ccgcttgcgc
      181 atcgtcagtt ttggcgaaga aaggcccgtt tgccagtacg acaacgaaga ttgctggcag
      241 agaaaccgac gcgccgattt ccgaaagcaa taggttgcga tccgacgtca actttggttg
      301 cgttgtacgc gacacaattt accatttcaa ctaagggcgg ctctcaccgc ccttagtgtt
      361 atgaggtgcc tttatgaaga acaaaattat tcccgctttt ttgttggtcg ttttattttc
      421 cacctcgttg gtttatgcca ggaaaaatga tgattcagcc tacgcccctc aggattacga
      481 caccatgcgc actgccattg ccaatcaagg caccagtatg gccaacatgc aaagtcagat
      541 cagccagatc acaggcagtc tccaaaccct cacaggtgat gtgaatcgta cttacaaaga
      601 aaacagggac caagaaaaaa ccatcgcaga cgcacataac cgcatgcaat ccctcgaaga
      661 caaaatgacc atcctgacca ctcaattggc cgaactgcag accgaagggt tcctgaagcc
      721 ccaggcctcc aaacgcctga gcgaatacaa agaatacgca acggggctgg aatttgtcaa
      781 cgcggcccaa tacgacaaag ccgttcaggc catgcaaagt tttcaaagca aaaatcccaa
      841 gagcatatac aaaagctttg cccaattctg gattggagag gcctattacc aacaagccga
      901 ttatcagatg gccatcaaag aatttcaaaa attattcgag ctcaatccac aatctgccaa
      961 ggcccccacg gccctgtaca aacaagggct ctcctttgct caactcaaat cctacgaaga
     1021 cgccaaggcc ttttttgcca acgtgatccg cacgtaccct gctagcctgg aggctattca
     1081 ggcgagttca caactcagaa gaatcgaaaa cgtctctgag ctgaacagac aacaaaatag
     1141 ggaattgaat ctggtccagt gatgggcctg acaacggggc cacttgactc catgaatgta
     1201 ttgcaacgct tagccttctc accccttttc acccagctgg tcatcacacg ccgttgtaac
     1261 ctgagttgtt cctactgcaa tgaattcgac cgaacctcca cccctgtacc cacagagact
     1321 ctcaaacaaa ggatttgcca gatcaaagat ctgggttctt tttcgatttg cctcacgggc
     1381 ggtgaacctt ttttacaccc cgatctatgt ggactcctca gcttttgccg acacgagcag
     1441 aagtttttca gaaccagcat gatcaccaac gggtatttcc tcacgcccg
//
LOCUS       DATDUN010000513         2112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_149688, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000513 DATDUN010000000
VERSION     DATDUN010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149688"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1567
                     /locus_tag="VJC18_07130"
     CDS             290..1567
                     /locus_tag="VJC18_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45192.1"
                     /translation="MFGCKLLAVSRRLFVMSLVFGLWSLVFGCSTFYGGTTSSEGEQN
                     ITAAPTEIQVMADVNTVGYRTITFTNQSKTDNYEITNMAFVDNDCGAYSLYNILDEAG
                     NMLYQTGDDIAVSVVPGARVDINIRFSPLKCAVTQYTTTFIIYYLSGEAEKTSAVSLV
                     TTVNDIAPDTVVCEPDTKTYYDEYDNPTERNLPALPTGQQYYLRVDKLSAFIQTTGAF
                     AAYATQVSTHLYLDVIPEADRFKPVYIPFTTDDEGNIVVSEIDSCVGFTMPTPITDTF
                     FIGAPVSVSTESEFAGSIDRDENAGRFEVPNLILRMASYINNTNSLLQSTEGYFDVNI
                     QVANLTSGTTDANPFILDVPEGVDDEGFDLLNIVDDTLVGKDIRHGTVTLVGVGTFLD
                     DDNTKLSNEAFSGIIDNEAYLFIQIQGLVTQAY"
     gene            1786..>2112
                     /locus_tag="VJC18_07135"
     CDS             1786..>2112
                     /locus_tag="VJC18_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45193.1"
                     /translation="MGLTACGNNDVSPAAPMIEPTPDFTPSEDTSLADMVINTFTDNG
                     IQITVEPEYIDFNASQGTISQNDELYEAFTITNNSGEARSFNFKIYSVSTGFSILDEN
                     KQNLGSY"
BASE COUNT          543 a          488 c          530 g          551 t
ORIGIN      
        1 ggacattggg taaaagatga agacccgatt cgtgatattt tgtgtgacta cggcattaag
       61 gatatttcga tcacaccgga gatcattttt gataacgacg aaggttacat tcatctctcg
      121 gcggatttgt attttgaatt ggaaagttgg atcactaatg agaacgaaga ataaagacca
      181 aagaaattcg aggaatcgtg ttcacgtgtt cacgtgttca tgtgttcacg atcgacagga
      241 aagctcaagg atcaaaggcc aacggtcaac agccaacggt caactgccta tgtttggctg
      301 taagctgttg gccgtgagcc gtcggctatt tgttatgtct ttggtctttg gtctttggtc
      361 tttggtcttt ggttgctcca ccttctacgg tggcaccacc agctccgaag gtgagcagaa
      421 catcacggcg gcgcccacag agattcaggt gatggccgat gtcaacacgg ttggttaccg
      481 cacgatcacg tttaccaatc aatccaagac ggataactac gaaatcacga atatggcctt
      541 tgtggataac gattgtgggg cgtattctct atacaacata ttggatgaag cgggaaacat
      601 gctctatcag acgggtgatg atatcgccgt aagcgttgtg ccgggggcca gggttgatat
      661 caatatccgg tttagtcctt tgaaatgcgc ggtcactcaa tacaccacca cttttattat
      721 ttattatctg tctggtgagg ccgaaaaaac ctcggctgtg tctttggtga cgactgtgaa
      781 tgatatcgct cccgacaccg tggtctgtga accagacacc aaaacctatt atgatgagta
      841 tgataatccg acagaaagaa atcttcctgc cttgccaaca gggcaacaat actatttgcg
      901 ggtggataaa ctgagcgcgt ttattcagac cacaggggcg tttgcggcgt atgccacaca
      961 ggtgagcacg catctttatc tcgatgtgat tcccgaagca gacaggttca aaccagtcta
     1021 catacccttc accactgatg atgaggggaa cattgttgtg agtgagattg attcttgtgt
     1081 tgggtttacc atgcccacgc cgatcacgga cacatttttt attggtgccc ctgtcagcgt
     1141 atcgacggaa agtgagtttg cagggtctat cgatcgtgac gaaaatgctg gtcgtttcga
     1201 ggtgccaaac ttgattttgc gcatggcatc atacatcaat aacaccaatt cgcttctgca
     1261 aagcacagag ggttatttcg atgtgaatat tcaagtggcc aacctcacga gtggaacaac
     1321 ggatgccaat ccctttatcc tcgatgtgcc tgagggcgtt gacgatgagg gttttgatct
     1381 gctcaatatt gtcgatgaca cactcgtggg caaagacatc cggcacggga cggtcacgtt
     1441 ggtgggggtg ggcacgtttt tggatgatga taacaccaaa ttgtcgaacg aggcgttttc
     1501 gggaatcata gataacgagg cctacctctt tatccagatt cagggtttgg tgacgcaggc
     1561 gtattaacga aaggacccat cgactgcgct cagggcaggc gatggacgac aagaatgcta
     1621 gaacgaactc acgaactcac gagctcacga gctcacgatc gacaaaaaag cccaaaggga
     1681 tgtttttcct ttcagctttc agctttctgc cctgagcgag cgaaacgagt cgaagggttc
     1741 agctttcagc ttgtcttatt gactttggtc attggacttt ggactttggg cttaactgcc
     1801 tgtggcaaca acgatgtctc ccccgccgcg ccgatgattg agccaacccc cgattttaca
     1861 ccatctgagg acacctcgct ggccgatatg gtgatcaaca cctttacaga caacggcatc
     1921 cagatcacag tggagcccga atacatcgat tttaacgcgt ctcaaggcac aatttcccag
     1981 aacgacgagt tgtacgaggc cttcaccatc accaacaatt caggggaggc acgctccttc
     2041 aatttcaaga tctattctgt ctctacgggt ttttcgattc tcgatgagaa caaacagaat
     2101 ctggggagct at
//
LOCUS       DATDUN010000514         5790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_397631, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000514 DATDUN010000000
VERSION     DATDUN010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397631"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /gene="scpB"
                     /locus_tag="VJC18_07140"
     CDS             complement(<1..1057)
                     /gene="scpB"
                     /locus_tag="VJC18_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HLD45194.1"
                     /translation="MDDNIRPIIKATLEAMLYVATQPVPMQKLLRRARTVAKLEMEKI
                     KEHQLKLQWPQEPQGENTEETEDCFLEPVVEICTENEAHCIDEIAPCEEASEQIENRS
                     EAEAELDPIESGQTCSEEGDAESGDIVMAQLIKKQQELELEISTADIKAILLEIQDAF
                     SDKNHGIELITVAKGYQFRTKYNISQYLRDEKIQAPSRFSPSGLETLAIIAYQQPVTR
                     QKIEEIRGVDSGGVLKTLLDKDIVRLVGRSEEPGRPLVYGTSRKFLEIFGINQLKDLP
                     NLHDYQSLELSALKSDENAQTDRQSSRIDLSDLLDDGVSHLTEEEEEILGHLDESIKN
                     VRRVEKHIVDMHFKQDAA"
     gene            complement(1089..1883)
                     /locus_tag="VJC18_07145"
     CDS             complement(1089..1883)
                     /locus_tag="VJC18_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HLD45195.1"
                     /translation="MKETITLQLDIFEGPFELLLYLIKKNDLEISRVSLAQVTDQYLQ
                     YLDALKDINVDLASDFLYMAAELALIKSRMLLPNESAQLEDEEDATAYDLIAKLKELE
                     QYKLAAQDLKGRVWLNRDVFTRGSFLEETDEAQDAAGKKADHFDVDPMDLIKVFSDLL
                     TKLPKEKRDHHVLIDRVSVADRIYEILESLKDADSVLFADLFTREKTRMAIVVTFLAI
                     LEMARLRMIRIYQMSHFEAIRVQRKMEIGSQVMDNNDMLKDIESYR"
     gene            complement(1886..2878)
                     /gene="trpS"
                     /locus_tag="VJC18_07150"
     CDS             complement(1886..2878)
                     /gene="trpS"
                     /locus_tag="VJC18_07150"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HLD45196.1"
                     /translation="MDQIIVSGTRPTNYLHLGNYHGALENWVKLQQQAQFKECFFFVA
                     DWHALTTGYDNTDEIKYFSQQMVIDWLAFGLNPDRATIFVQSQVPEHAELNLLLSMIT
                     PLGWLERVPTYKDQQQELSHKDLATHGFLGYPVLQTADVALYNATHVPVGQDQVPHIE
                     LSREIVRRFNFIFKTELLIEPQALLTNVPVLPGTDGRKMSKSYHNSIYLKDTPDEVQK
                     KILPMMTDPARQRRTDAGDPAKCPVFSYHKIYSGELIKQEVAHGCQTAGIGCVDCKKK
                     LLSQMETCLAPYRQKRLDLATQEKQVRDVIEAGNAKARRIATTNMNQIKDIVKI"
     gene            2986..3180
                     /gene="ssrS"
                     /locus_tag="VJC18_07155"
     ncRNA           2986..3180
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VJC18_07155"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3222..3794
                     /locus_tag="VJC18_07160"
     CDS             3222..3794
                     /locus_tag="VJC18_07160"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HLD45197.1"
                     /translation="MPKKDIRQNKLNERLSLDPVKIAEYSQQIQDRIMKTPFWPKTGK
                     IGLYYPIKNEVLTLSLFQKALETGLQVYFPRVEQGISFYEVNGPDELEKGSWGIAEPQ
                     KHCDPLIEEENLDLVIVPGVAFSKSCFRIGYGKRFYDQFLDNKLHKTTAIGLAYDFQV
                     LDDFPVDSWDQPLHGIMTEKNYYSLHVQKI"
     gene            3810..4613
                     /locus_tag="VJC18_07165"
     CDS             3810..4613
                     /locus_tag="VJC18_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HLD45198.1"
                     /translation="MNATKLKIIFLGDLFGEPGRRAVGQILPELKIKFTPDLVFANCE
                     NAANGRGISRKIANELEAFGFDLITTGNHVYQVKDIYPYLNEEGCKIIRPYNFPRNSP
                     GRGVAVVSAPSGLRVGVINLMGNLFMQTNVDCPFHAIDSALSELKDETDIVLVDFHAE
                     ATSEKRAMGWYLDGKVQLCVGTHTHILTADDEILPGGTAYITDIGMCGPYDSVIGMDK
                     EIIINKYRTLIPRRFEVAKNDLRVTGIYCEIDLENKKATKINRIDERVS"
     gene            4610..>5790
                     /gene="tyrS"
                     /locus_tag="VJC18_07170"
     CDS             4610..>5790
                     /gene="tyrS"
                     /locus_tag="VJC18_07170"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HLD45199.1"
                     /translation="MKSVNEQLNIIKRGVVEIIDEKELTKKLSENRPLVIKAGFDPTA
                     PDLHLGHTVLINKLKQFQDLGHDVHFLIGDFTGMIGDPTGVSETRKVLTAEQVKENAK
                     TYTDQVFKILCPSKTKVMFNSDWLGKMGVIAFAELGSKLTVARMMERDDFKKRFTENK
                     DISILEFYYPLMQGYDSVAIKADVELGGTDQKFNLLMGRTIQKRYGMDSQIVLTMPLL
                     EGTDGIRKMSKSYGNYIGIKDTPQDMFGKLLSITDELMWRYFELLSFKPMPEIEALKS
                     SVLKEGLNPKEAKVLLAKEIITRFHNEQAAEQAERDFNQIFAKKELPDDIDVFEYSLS
                     NGPVTLIDLIMTAGMTASKGEARRLIAQDGIKVNQEKISDEKLSFDSPCEFIVQVGKR
                     K"
BASE COUNT         1531 a         1457 c         1307 g         1495 t
ORIGIN      
        1 aagcggcgtc ctgtttgaaa tgcatgtcga caatgtgttt ttcgacgcga cggacatttt
       61 taatggattc atccaaatgt cccaatattt cttcttcctc ttcggtcagg tgcgacaccc
      121 cgtcatcaag caaatccgac aaatcaatcc gtgaagactg tctgtcagtc tgtgcgtttt
      181 catcggattt cagtgccgat aattccaaag attgataatc gtgcaaattg ggcaaatcct
      241 tcaattgatt gattccgaat atttccaaaa attttctgga tgtcccataa accaggggtc
      301 tgcctggttc ctcactacgc ccaaccagac gcacgatgtc tttgtccaat aaggttttga
      361 gcacaccacc tgaatccacc ccgcgaatct cctcgatctt ctggcgcgtc acaggttgtt
      421 gataggcaat gatggccagc gtttcgagtc cagaaggaga aaaacgcgac ggtgcctgaa
      481 tcttttcgtc tcttaaatac tgtgagatgt tgtacttggt gcggaattga taacccttgg
      541 cgactgtgat caactcaatg ccatgatttt tatcactgaa cgcatcctga atttccaaaa
      601 ggatcgcctt gatgtcggcg gtactgattt ccagctcaag ttcctgctgt ttttttatca
      661 gttgcgccat gacaatgtca cccgactcgg cgtctccctc ttcggaacag gtttgacctg
      721 actctatcgg atccagctct gcctctgctt ctgacctgtt ttcaatttgt tcagaggcct
      781 cttcgcatgg cgcgatctca tcgatacagt gagcctcgtt ctctgtacag atttcgacca
      841 cgggctccag aaagcagtct tctgtttcct ctgtgttttc tccctgaggt tcctgcggcc
      901 attgcagctt aagctggtgc tccttgattt tttccatttc cagcttggcg acagtccgcg
      961 cccgtctcaa taatttctgc atgggcactg gctgagtggc tacatacaac atggcttcca
     1021 gggtcgcctt gatgattggt ctgatgttgt cgtccataaa attctcacta actcccacag
     1081 agggaactct accgatatga ttcaatgtcc ttcaacatat cgttgttatc catcacctga
     1141 gagccgatct ccatcttgcg ttgcactcga atagcttcga aatgactcat ttgataaatg
     1201 cgaatcatcc tcaggcgtgc catttccaat attgccaaaa aagtcaccac aatcgccatg
     1261 cgggtttttt ccctggtaaa aagatcagca aacaacacac tgtccgcatc cttgagagac
     1321 tcaagaattt catatatgcg atcggcgact gaaaccctgt cgatcaggac gtgatggtca
     1381 cgcttttcct tgggtaactt ggtcagcaaa tcggaaaaga ccttgatgag atccatgggg
     1441 tcgacatcaa aatgatctgc tttcttgcca gccgcatcct gagcctcatc tgtttcctcc
     1501 aaaaacgagc cccgggtgaa aacatcccgg ttgagccaga cacgtccctt taaatcctga
     1561 gcggccaatt tgtactgctc cagttctttg agtttggcga tgagatcata ggccgtggcg
     1621 tcttcctcgt cttccagttg agcactctca ttgggcaaca acatccgcga tttgatgagt
     1681 gcgagttctg ccgccatgta caaaaaatca ctcgccagat ccacattgat atccttgagg
     1741 gcgtccagat actgcaaata ctgatccgtc acctgcgcca aggacacccg cgatatctcc
     1801 aggtcatttt ttttaatgag gtacaacagc aactcaaagg gaccttcgaa gatatcaagt
     1861 tgtaatgtga tggtctcttt catgtttata tctttacgat gtccttaatc tggttcatat
     1921 tcgttgtggc aattcttcgt gccttggcat taccggcctc gatcacgtca cgaacctgtt
     1981 tctcttgcgt cgccaagtca agtcgttttt gacgatacgg cgccaagcac gtttccatct
     2041 gactcaaaag ttttttcttg caatccacgc acccgatacc cgccgtttga cacccgtgtg
     2101 ccacctcctg cttgattaat tctcctgaat aaattttgtg atacgaaaac acggggcact
     2161 tggcgggatc accggcatct gtgcgcctct ggcgggccgg atcggtcatc atgggcaata
     2221 tctttttttg cacctcgtct ggggtatctt tgagataaat gctgttgtgg taacttttgg
     2281 acatttttcg tccatcagtg cccggcaaca caggcacgtt agtcaacaag gcttgtggtt
     2341 caatcaacaa ttcagtcttg aaaataaaat tgaaccgtct gacaatctcg cgagacaact
     2401 caatatgcgg cacctgatcc tggcctaccg gaacatgtgt ggcgttgtac agagccacgt
     2461 ccgctgtttg caaaacggga taacccaaaa aaccatgagt cgccaaatct ttgtgagaca
     2521 attcctgttg ttgatcttta taggtgggaa cacgctccag ccaccccaag ggagtgatca
     2581 tggacaacaa caagttcagc tcggcatgtt cgggaacctg cgattgaaca aaaattgtcg
     2641 cccggtcggg attgagacca aaggccagcc aatcaatcac catctgctgg gaaaaatatt
     2701 tgatttcgtc ggtgttgtcg taacccgtgg tcagagcatg ccaatccgcc acaaagaaaa
     2761 aacattcctt gaactgggcc tgttgctgca atttgaccca attttccaac gcaccatggt
     2821 aattacccaa atgcaaataa ttggtgggcc gcgttcccga tactatgatt tgatccatac
     2881 agatacctca aggaagccct gtctaaatga tctcacgccc aaaaaacaag gagataatca
     2941 attgcgaaaa aacttgcagc aaatcagcca aagaggcata atgacctcgc cctgcgttgt
     3001 gcgcagtagt atcaaaaaaa tggaccaaca tattctttgg gaaccgtcac tgttcctggt
     3061 gtgcatgccg gcacaagggt tctgccctta agtccggaag cctaaaggga ttaccatggt
     3121 gcccacctgc atagcaggtt ccaattttac gtactacctg tcggcaaagg cggggcgttt
     3181 tttattgacc ctccgccagt aaccctacta gtagtccgcg cgtgccaaag aaggatatcc
     3241 gacaaaacaa actcaatgaa cgtctttcgc ttgatcctgt gaagatcgct gaatattcac
     3301 aacaaattca ggacagaatc atgaagaccc ccttttggcc caaaaccgga aaaatcggct
     3361 tgtactaccc catcaagaat gaggtattga ctctgtccct ctttcagaag gcattagaaa
     3421 ctggcctgca ggtttatttc ccccgtgtcg aacaggggat cagtttctat gaggtcaatg
     3481 gacctgatga actcgaaaaa ggttcgtggg gaattgctga accacaaaaa cactgtgacc
     3541 ccctcatcga ggaagaaaat ctggatttgg tgattgttcc gggcgtagcc ttttcaaaaa
     3601 gctgttttcg catcggctat ggaaaacgct tctacgacca gtttctggac aacaaactcc
     3661 acaagaccac ggccatcggt ttggcatacg attttcaggt gttggacgat tttccggtcg
     3721 attcctggga ccaacccttg catggtatca tgacagaaaa aaattattac tcgcttcatg
     3781 ttcaaaaaat ctgattcttt ccggtaatta tgaacgcaac caaactcaaa atcattttcc
     3841 tgggcgatct gtttggagaa cctggccgac gcgccgtggg gcaaattctg cccgaattaa
     3901 aaatcaaatt cacgcctgat ctcgtttttg ccaattgtga aaacgcggcc aatggacgag
     3961 gaatctcccg caaaattgcc aacgagctgg aagcttttgg tttcgacctc atcaccacgg
     4021 gcaatcacgt gtatcaggtc aaggacattt acccctacct caatgaagaa ggctgcaaaa
     4081 tcatccgtcc ttacaatttt cccagaaatt ctccgggacg tggtgtggcc gtggtcagcg
     4141 caccatccgg tctcagggtg ggcgtcatca atctgatggg caatcttttt atgcagacca
     4201 atgttgactg cccatttcac gccattgatt ctgccttaag tgaactcaaa gacgagaccg
     4261 acattgtcct ggtggatttt catgctgaag caacctcaga aaagcgcgcg atgggctggt
     4321 acctcgatgg caaggtgcaa ctctgtgtgg gaacacacac gcacatcctg actgccgatg
     4381 acgaaatcct gcccggcggg accgcgtaca tcacggacat cggcatgtgc ggcccatatg
     4441 attcggttat tgggatggac aaagagatca tcatcaataa gtacaggacc ctcatccccc
     4501 gccgcttcga agtcgccaag aacgacttgc gggtcaccgg tatctattgc gaaattgacc
     4561 tcgaaaacaa gaaagccacc aagatcaatc gcattgacga aagggtgtca tgaagagcgt
     4621 taatgaacaa ttgaacatca tcaagcgagg cgttgtcgaa atcatcgacg aaaaagaact
     4681 caccaagaaa ctctccgaga accggccatt ggttatcaaa gccgggttcg atcccacagc
     4741 tcccgatctg catctggggc acacggtcct catcaacaaa ctcaaacagt ttcaagattt
     4801 gggtcatgat gttcattttt tgattggtga ttttacgggc atgattggag acccaacagg
     4861 tgtctccgag acacgcaagg tcctgaccgc tgaacaggtc aaggaaaatg ccaaaacgta
     4921 caccgatcag gtcttcaaga ttttatgccc ctcaaaaaca aaagttatgt tcaacagtga
     4981 ctggctgggc aaaatgggtg tgattgcctt tgccgagtta ggatcaaagc tcactgtggc
     5041 caggatgatg gagagggacg attttaaaaa gagatttacc gaaaacaagg acatcagtat
     5101 tctcgaattc tattaccctc tgatgcaggg ctacgattct gttgccatca aggccgatgt
     5161 tgaactgggc ggcaccgatc aaaaattcaa tctgctcatg ggcagaacaa ttcagaagcg
     5221 ttacggaatg gattcgcaga ttgtgctcac catgcctctg ctggaaggca ctgatggcat
     5281 cagaaaaatg agcaaatcct acggcaatta cattggaatc aaggatacac cgcaggacat
     5341 gttcggcaaa cttctctcca tcaccgatga gctgatgtgg cgttattttg aacttctgag
     5401 tttcaagcca atgccggaga ttgaggccct gaaatcctca gtgctgaaag agggtctgaa
     5461 tcccaaagag gcaaaagtgc tgttggcgaa agaaatcatc acccgttttc acaatgagca
     5521 agcggcagag caagccgaac gtgatttcaa ccagattttt gccaaaaaag aattgcccga
     5581 tgacatcgac gtttttgaat actccctttc aaacggtccg gtgactttga ttgatttgat
     5641 catgacagct ggcatgacag ccagcaaggg agaagcccgg cggctgatcg ctcaggatgg
     5701 catcaaggtc aaccaggaaa aaatcagtga cgaaaaactc agcttcgaca gcccctgcga
     5761 gttcattgtg caggtgggca aaagaaaatt
//
LOCUS       DATDUN010000515         5452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_440694, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000515 DATDUN010000000
VERSION     DATDUN010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440694"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VJC18_07175"
     CDS             complement(<1..994)
                     /locus_tag="VJC18_07175"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLD45200.1"
                     /translation="MSEHKPICYKKRDDVPELVMSQASSLWNQTGSWRYIRPRYQDSV
                     PPCNHTCPAGNDIEGFIRHYLAGNTDEAWQVLKEENPFPRVCGRVCYHPCEGACHRGQ
                     FDRAVAINDLERFVGDQPPSVRKIAKLKDSSEKSVAVIGAGPAGMTVAYHLARLGHQV
                     TVYDAHAKAGGVLRYGIPAYRLPKDVLDDELNDIFSLGVKFVGEKTLGVNLQWQDLNA
                     FDAVFVATGAHQSRQLGVPDENSEGVMSGLHFLTKVAASKTNFLGDHVVVVGGGNTAI
                     DAARSALRLGSQVTICYYRSRQEMPAHPEEIADAELEGAEIRFLCQPLRIIKTNN"
     gene            complement(1004..1552)
                     /locus_tag="VJC18_07180"
     CDS             complement(1004..1552)
                     /locus_tag="VJC18_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HLD45201.1"
                     /translation="MIEIRFHGRGGQGAVIASKILACAFFAEGKWVQAFPKFGVERRG
                     APVEAFLRMDQEKILLRNNIYEPDHVIILDHTLIEATAVTQGLKKGGWVIINTSKAPK
                     DFPKLTDFKVACVDANILAMRHRLGSRSSPIVNTAILGAFAKATELVRLDAIVKAIKD
                     DVPIKSDENALAAEEAFAATMI"
     gene            1701..2075
                     /locus_tag="VJC18_07185"
     CDS             1701..2075
                     /locus_tag="VJC18_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45202.1"
                     /translation="MSEKSQKTGWTYGGLGAFCWLFILGIVLLIQKNFHGSFFAFVFF
                     AMGIAYLIEYAPWKYPAVPFWKIYLGLIALLFLSTAVLMCLWDDKPAIAYERFTSLVY
                     LFPMCIPMVVFGKKTWNEMQKK"
     gene            2072..3184
                     /locus_tag="VJC18_07190"
     CDS             2072..3184
                     /locus_tag="VJC18_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLD45203.1"
                     /translation="MNIIFDLGGVLVDWEPQKIFLEFFKDPALAQKIYLALYTHADWA
                     ELDRGTLSAWEAAERVAQRTGVAAETVQQFLLNFATLIKPKNETIKLIQNLKNDGHKL
                     FCLSNINPWTFEYLNKHFDFFKHFLGIVISSHEKLVKPDPKLYARLVKRYNILPQETI
                     YIDDLKENLGPAQELGMPAILFQSSPQCEQEIQKLISQVRLQAAHNTLNFIAGKFSWE
                     GYIANYQNTEDKVIAQFVLAAEKTMSSGFNPKQIREARLTVLSHIETSHEEHKTDYSK
                     EFELQFNKAKRCGYGLAACILFPFILNSFLHDRQNVSPGLFKTVLILALAIMLFLIPK
                     AVNLMRCPNCKKMSLRLSDDFCAHCGVRLRNPKGRN"
     gene            complement(3192..4205)
                     /locus_tag="VJC18_07195"
     CDS             complement(3192..4205)
                     /locus_tag="VJC18_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLD45204.1"
                     /translation="MLLTVTKDMREVLFRRVFGHAVFKQSLLYHHKVCGVVLFELAGE
                     RALVDEVINRTQNDQELCGITVLKQESDAATGTVIPLNRLYYGDSFVDVFSEADVETY
                     RRAYAVKIRQNTQTPIAKHINKRLSLPLSRILASQTISPNIISAIALLWSLVGALCLL
                     TREYYVLGFLCFQINSVLDGCDGEVARFNFAFSEFGKKLDVYCDYLTTITIIVFETVG
                     FYLLCPNLWVLALAGGSLALLVMIGVLSFMSKKSLGNSGSFNDLEVLCHQRLAHPQNL
                     WDRVNSLFLFVSHRDFYILAILILAMMGQFAVIHIFLSLLCLSWTMLSLHTYRITHTA
                     LPQ"
     gene            complement(4217..4927)
                     /locus_tag="VJC18_07200"
     CDS             complement(4217..4927)
                     /locus_tag="VJC18_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLD45205.1"
                     /translation="MKHFKFKDVFTLANLFLSFFSVILMFSGKFEEASYIILINILIL
                     DILDGLVARITKTGNDFGKYFDSITDFVGSSVIVSYFIFSALSPTNYYLALVCGFAFL
                     LTGVMRDVRSRLETVAHKGFFIGLPRNLASVLIVALFNSHLFAKYPLSAVLYLGILCT
                     MQLSYAPYVGNHKPTLFAMPRVRNYLLVGIVYAGVHAYFGYFWDSMVTFMCGYLLSPY
                     FLASPAVWEDIRRQRVMG"
     gene            complement(5039..>5452)
                     /locus_tag="VJC18_07205"
     CDS             complement(5039..>5452)
                     /locus_tag="VJC18_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45206.1"
                     /translation="NHAEIGLRTSVNPTYHQLYELLRKKPFRGPIKYVLVTSFDGVVE
                     LRLINGGTTLGRHFTHAHLLCEGDVVIACGMLDMQVSTGRPTIIIDGESTNFPTTEES
                     VTLSGTDLDHMTKAQPCGLKVARGTLKSLYPQCSI"
BASE COUNT         1536 a         1405 c         1187 g         1324 t
ORIGIN      
        1 tgttatttgt tttgatgata cgcaacggtt ggcacaaaaa tctgatttcg gcaccttcaa
       61 gctcggcatc ggcaatttcc tctgggtgtg ccggcatttc ctggcgcgag cggtaataac
      121 aaatggtgac ctgactgcca agtcgcagag ccgatctggc cgcatcaatc gccgtattgc
      181 cgccaccaac cacaaccaca tgatcgccca gaaaatttgt ttttgaagcg gccacttttg
      241 tcaaaaaatg gagcccactc atcactcctt cgctgttttc atcgggcaca cccagttgtc
      301 gactctgatg agcaccggtg gccacaaaga cagcatcaaa agcattcagg tcctgccact
      361 gcagattgac cccaagtgtt ttttcaccaa caaatttgac tcccaaagaa aaaatatcgt
      421 tgagttcgtc atccagaaca tctttgggca ggcgataggc tgggatcccg taacgcaaca
      481 ccccacccgc tttggcatga gcatcataaa ctgtcacctg atgacctaat cttgccagat
      541 gatacgccac ggtcatgccg gcaggacctg ccccaatgac agcaacagat ttttctgaag
      601 aatcttttag tttggcaatt tttctgacag agggtggttg gtcaccaaca aaacgttcca
      661 gatcgttgat ggcaaccgcc ctatcaaact gccctcgatg gcaggctcct tcgcacgggt
      721 ggtaacacac acgaccacac acacgtggga acggattttc ttccttcaac acctgccacg
      781 cctcatctgt gttgcccgcc agatagtgcc gaataaaacc ttcgatgtcg ttgccagccg
      841 ggcacgtatg attgcacgga ggcacactgt cctgataacg cgggcgaatg tagcgccacg
      901 atcccgtttg attccaaagg ctcgacgcct gcgacatcac aagctcaggc acgtcgtccc
      961 tttttttgta acaaattggc ttgtgttctg acatgagaat ccctcaaatc attgttgccg
     1021 caaaagcctc ttcggcggcc aacgcatttt cgtcggactt gatcggcaca tcgtctttga
     1081 tggctttcac aatcgcatca agccgcacca gttccgtggc cttcgcaaaa gctcccagaa
     1141 tcgccgtgtt gacaatcggg gaactgcgcg aacccaaacg atgcctcatg gccaagatat
     1201 tcgcatccac acaggccacc ttaaaatcag tcagtttcgg aaaatctttt ggtgcttttg
     1261 acgtattgat gatcacccag ccaccttttt tgagcccctg cgttaccgcc gtggcctcaa
     1321 tgagagtgtg atcaagaatg atcacgtgat caggctcata gatgttattg cgcaaaagaa
     1381 tcttttcctg atccatgcgc agaaaggcct cgaccggagc cccgcgacgt tcaaccccaa
     1441 atttggggaa ggcctgcacc cacttgcctt cggcaaaaaa agcacaggcc aaaattttgg
     1501 aggcaatcac tgccccctgt ccgccgcgcc catggaaacg aatttcgatc ataacgaact
     1561 cctcacgaac tcttttcgta tgacgaagca aggtaaggat gcaacagaaa atctcaacaa
     1621 tttataataa ttgatctttt tacattattt gttagtatca acctgtcgtt tgcatataaa
     1681 caggtagaat cgaggcacat atgagcgaaa aaagtcagaa aaccggttgg acttatggtg
     1741 ggctgggggc cttttgctgg ttatttattc tggggatagt tttactgatt caaaaaaatt
     1801 ttcacggcag tttttttgcc tttgtatttt ttgccatggg gatcgcctac ctcatcgaat
     1861 acgcgccttg gaaatatccc gccgtgccct tttggaagat ttatttagga ctcatcgccc
     1921 tactcttctt atcgacagct gttctcatgt gtttgtggga cgacaagcct gccattgcgt
     1981 atgagcgctt cacttcgttg gtctatcttt tccccatgtg tatcccgatg gtagtgtttg
     2041 gcaaaaaaac ctggaacgag atgcaaaaga aatgaatatc atttttgatc tgggcggcgt
     2101 cctcgtcgat tgggagcctc aaaaaatctt tcttgagttt ttcaaggacc cggcattggc
     2161 tcaaaaaatt tacctggccc tgtacacgca cgcagactgg gctgaattgg atcgtgggac
     2221 gttgtcagcc tgggaggccg ctgagcgcgt ggcacaaaga acgggcgtcg cggccgagac
     2281 cgttcagcag tttcttctca atttcgccac tctcatcaaa ccaaagaatg aaacaatcaa
     2341 gctgatacaa aacttgaaaa acgatggaca caaattattt tgtctctcta atatcaaccc
     2401 ctggacattt gaatacctga ataaacactt tgattttttc aaacattttc tcggcatcgt
     2461 catttccagc catgaaaagc tcgtcaagcc agatccaaaa ttatacgctc gtttggtcaa
     2521 acgctacaac atcctgcctc aagaaaccat ttacattgac gacctgaaag agaatcttgg
     2581 tcccgcgcag gaactgggca tgccagccat tttgtttcaa tcatcccccc aatgcgaaca
     2641 ggaaattcaa aagctcatct cacaagtacg cttacaggcg gctcacaaca cgctcaattt
     2701 cattgcaggc aaattttcgt gggaaggtta cattgccaac tatcagaata cggaagacaa
     2761 ggtcattgcc caatttgtgt tggcagccga aaaaaccatg tccagcgggt tcaacccaaa
     2821 acaaatcagg gaggctcgtc tcacagtctt gagtcacata gagaccagcc acgaagaaca
     2881 caaaacagat tattccaaag aatttgaact gcaattcaat aaagcaaaac ggtgcggtta
     2941 tggtctcgcc gcatgcattt tgttcccctt catcttgaac agttttctgc atgatcgcca
     3001 aaatgtgtcc ccgggcctat tcaagacagt actaatcctg gctctagcca tcatgttgtt
     3061 cctcattcct aaagccgtca atctcatgag atgcccgaat tgcaaaaaaa tgagtctccg
     3121 gctgagcgac gatttttgcg ctcactgcgg tgtccgactg aggaatccga aaggccgcaa
     3181 ttgacagaac cttattgcgg caacgctgtg tgtgtgattc gataggtgtg gaggctcagc
     3241 atggtccaac tcaggcagag caaggataaa aaaatgtgaa tgacggcaaa ttgtcccatc
     3301 atcgccaaaa tgagaatggc cagaatataa aagtcgcgat gactcacaaa aagaaacaat
     3361 gagttcacac gatcccaaag attttgtgga tgggccagtc gttgatgaca aagcacttcc
     3421 aaatcattga aactgcctga gttacccaaa ctctttttgc tcataaaaga caaaacccct
     3481 atcatcacca gcagtgccag actgcccccg gccagcgcca agacccacag gtttggacac
     3541 aacagataaa atccgactgt ttcaaagaca atgatggtga tcgtggtgag gtaatcacaa
     3601 tacacatcga gttttttgcc aaattccgaa aaagcaaaat tgaatctcgc cacctcgccg
     3661 tcacaaccat ccaaaacaga attgatttgg aaacacagga atcccagaac gtagtattcc
     3721 cgggtcagta aacacagagc ccctaccaga gaccacaata gggcgatcgc cgaaatgata
     3781 ttgggactga ttgtttgact cgccaggatc cgtgacagag gcaaggacaa tcgcttgttg
     3841 atatgtttgg cgatcggggt ttgcgtgttc tgcctgatct tgacagcgta cgccctgcga
     3901 taggtctcaa catcagcttc ggagaacaca tcaacaaacg aatccccata gtacaaacgg
     3961 ttcaacggga taacggtgcc agtagccgcg tcactctctt gtttgagtac ggtgatccca
     4021 cagagctctt gatcgttttg tgttctgttg atgacctcat caacaagcgc cctttctcct
     4081 gccagttcaa acaacacgac cccgcacacc ttgtggtgat acagtaaaga ttgtttgaac
     4141 accgcatgac caaaaacgcg tcgaaaaaga acctctcgca tgtcttttgt gacggttagt
     4201 aacatagcat tacccatcaa cccatcaccc gttgccttct aatatcctcc cacaccgcgg
     4261 gactggccag aaagtacgga ctaagcaagt aaccacacat gaatgtcacc atgctgtccc
     4321 aaaaataacc aaagtacgca tgaacgcccg catacacaat ccccaccaaa agataattgc
     4381 gcacacgggg cattgcgaac agcgtcggtt tgtgattgcc aacatacggg gcataggaca
     4441 attgcatggt gcacaaaatc cccagataca aaacagcact gagcgggtat tttgcaaaca
     4501 agtgcgaatt aaaaagcgcc acaatgagca cgctggccag attgcggggc aaaccaatga
     4561 aaaaaccctt gtgggccaca gtctccaacc gtgacctcac atcacgcata acccccgtga
     4621 gcaaaaaagc aaaaccgcaa accagcgcca aataataatt cgttgggctc aaagccgaga
     4681 aaataaaata ggatacaatc acactggacc ccacaaaatc ggtgatgcta tcaaaatatt
     4741 ttccaaagtc attaccagtt ttggtgatcc tggccaccag gccatccaat atgtcgagaa
     4801 tgagaatatt gatgaggatg atgtatgagg cttcttcgaa ttttcccgaa aacatcagga
     4861 tcacggaaaa aaaagacaaa aacagattgg ccaaagtaaa aacatccttg aatttgaaat
     4921 gtttcatatt catatgtgtt gccccataaa accaaaaaaa aattgtatca agcgtggaaa
     4981 taattcggtg accgcaacaa tgtgcaggac tcagtcatca gcgtgactgg ggtggttttt
     5041 agattgagca ttgcgggtat agacttttca atgtccccct ggcgacctta agtccgcaag
     5101 gttgggcttt ggtcatgtga tcaagatcgg tgcctgacag ggttacactc tcttctgtcg
     5161 tggggaaatt tgtactctcg ccatcgatga tgatcgttgg tcttcccgtc gatacctgca
     5221 tgtccagcat gccgcacgcg atcaccacgt caccctcaca caataaatgc gcatgtgtga
     5281 agtgtcttcc caaagtggtt cccccgttga tcaggcgcaa ctcaacgacc ccgtcaaacg
     5341 aggtcaccag cacgtacttg atggggcccc tgaaaggttt ttttctcaac agctcgtata
     5401 attgatgata ggtcggattc acggaggtcc gcaaaccaat ttcggcatga tt
//
LOCUS       DATDUN010000516         1522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_230689, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000516 DATDUN010000000
VERSION     DATDUN010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230689"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VJC18_07210"
     CDS             <1..805
                     /locus_tag="VJC18_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLD45207.1"
                     /translation="ENINFVKAHTSDGMCKGLFGLHASLTLSDKTLADCAQAARNLGV
                     GVHVHTAEDKADVIKAQKEHGVGVVERWENFGVLGSKTVLAHCVHISEHEMDLIARSK
                     TNVVHNPESNMNNAVGCADVIKMLGKGCLVGLGTDGMTSDMFQEAKFAHLIRKHEVRD
                     PRLGFMQAGELLFDNNYKILGNLFEGTLGRIQTGALADLIILGYDPPTPLTRDNFWGH
                     FLYGMNSSLVNTTIINGRVLMKDRVIAGIDEHEICARSRELARKLWTRI"
     gene            1006..>1522
                     /locus_tag="VJC18_07215"
     CDS             1006..>1522
                     /locus_tag="VJC18_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLD45208.1"
                     /translation="MNKYILTCIDCRKTFEPDAVDYVCPVCSQTQKPFQPLRGLLSVD
                     YDWARLGSTLDQEVFAENPMTGHGRYLDLLPLNNLNSLPALDVGPTPLDPVLRMRVAL
                     GLKNLFVKNDTLLPTGSFKDRASSVVVAKARELGKDVITTASTGNAATALAGMCASVG
                     MKSVIFVPETAP"
BASE COUNT          384 a          361 c          431 g          346 t
ORIGIN      
        1 agaaaacatc aattttgtta aggcgcatac cagcgacggg atgtgcaagg gtttgtttgg
       61 tctgcacgcc tcactcacgt tgtccgataa aacactggcg gactgtgcac aagcggctcg
      121 caatctggga gtgggcgttc atgtgcacac ggccgaggat aaagccgatg tgatcaaggc
      181 gcaaaaggaa cacggtgtgg gggtggtgga gcgttgggaa aactttggag tacttgggtc
      241 caagacagtg ctcgcgcatt gtgtgcacat ttccgaacac gagatggacc tcattgctcg
      301 gtcaaagacc aacgttgtgc acaatcccga gtccaacatg aacaacgccg tgggctgtgc
      361 cgatgtgatc aaaatgctgg gcaagggttg tctggtgggg ctgggcaccg atggcatgac
      421 gagcgacatg tttcaggagg ccaagttcgc gcacctgatt cgcaaacacg aagtgcgtga
      481 tccccgctta ggattcatgc aggcgggtga gttgttgttt gataataatt ataaaatcct
      541 gggcaatctt tttgagggaa ctttgggcag gattcaaaca ggggctctgg ccgatcttat
      601 tattctgggc tacgatcccc ccacaccact cacgcgcgat aacttctggg gacatttttt
      661 gtacggcatg aactccagct tggtcaacac cacgatcatc aatggacggg tgctgatgaa
      721 agatcgcgtg attgcgggga ttgatgagca tgagatctgt gccagatcga gggagttggc
      781 acgaaaacta tggacgcgaa tataaacgat ggtacgaaga aacgaaaaca cgcatgccca
      841 cagaagctcg aagagcgtag gtgggaggga caaaataatt gagcaatttg caatgggcaa
      901 tcgtagcaat tgcggcaatt gcaatgattg caacaattgc agtaattgct acagttgatg
      961 acgatccacg atccacgatc cacgatccac gaagaacgag gcactatgaa caaatacatc
     1021 ttaacctgta tcgattgcag aaagacgttt gaacccgatg cggtggacta tgtctgcccc
     1081 gtctgcagtc aaacacaaaa accctttcaa cccctgcgcg ggttgctcag tgtcgattac
     1141 gattgggcaa ggctgggatc taccctggac caagaggtct ttgccgaaaa tcccatgacg
     1201 ggtcatggac gatatctcga tcttctgcct ctcaataatc tcaactctct gccagcgttg
     1261 gacgtggggc ccacaccgct ggatcccgtt ttgcgtatgc gggttgcttt ggggctgaaa
     1321 aatctgtttg tcaaaaacga taccctcttg cccacgggat cttttaaaga tagggcatca
     1381 tcggtggtgg tggccaaggc gcgtgagctg ggcaaggacg tgatcaccac ggcgtctaca
     1441 ggcaacgcgg ccacggcgct cgctggtatg tgcgcttctg tcggcatgaa gagtgtgatt
     1501 tttgttcccg aaacagcccc cc
//
LOCUS       DATDUN010000517         1892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_108701, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000517 DATDUN010000000
VERSION     DATDUN010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108701"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VJC18_07220"
     CDS             complement(<1..250)
                     /locus_tag="VJC18_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HLD45209.1"
                     /translation="MLTLRNKLPEFVITTKLDELINWGRKSSVWYLLFGLACCGIELM
                     QTGGPRTDLDRFGSVFRATPRQSDLMIVAGTLTYKMARR"
     gene            complement(450..881)
                     /locus_tag="VJC18_07225"
     CDS             complement(450..881)
                     /locus_tag="VJC18_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HLD45210.1"
                     /translation="MMLFNYSNVIIFALVGIGFIFVTLFIGSLLRPHNPNPEKLKTYE
                     CGEEPVGAAWMNFNIRFYVIALVFVIFDVEVALMFPVVRVFREWVAKGDGVIALIEVF
                     VFILILLLGFYYVWKKGDLEWNKNISSKLNPSASSGQEGER"
     gene            complement(920..>1892)
                     /gene="ccsA"
                     /locus_tag="VJC18_07230"
     CDS             complement(920..>1892)
                     /gene="ccsA"
                     /locus_tag="VJC18_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLD45211.1"
                     /translation="NPSAVIDVIQQYGQAFRQQNPHYLVELTYHRLHLLHMALLAAMV
                     ALVLGFVLKNPHHPMTVSVLSLLFFLQAAMVVFRVLIAGRAPVTNMYETVLWVGLGAL
                     VFGGLLGWWRRERLFLLFGVLINLVSLFMMMFADRMLDPTLKPLAPVLRDNFWLATHV
                     TTITISYAAFGLSWLIANYYMIKGIASKIGYEEGKHLNEMTYDCLRIGVVLLSAGILL
                     GAVWADYSWGRFWGWDPKETWSLVALMAYLAVLHGRYAGWIGDESFLPYVALAFLTIV
                     IAWFGVNYVLASGLHSYGFSRGGATAILALIMAQIILFLLWKIRKIF"
BASE COUNT          571 a          535 c          401 g          385 t
ORIGIN      
        1 cgcgcctggc cattttgtag gtgagcgttc ccgccacaat catgaggtct gattgacggg
       61 gcgtggcgcg aaacacggac ccaaatcggt ccagatcagt acgcggcccg cccgtttgca
      121 tgagttcaat gccacagcag gcgagtccaa acagcagata ccacacggac gatttgcggc
      181 cccagttgat gagttcatca agcttggtgg tgatgacgaa ttcgggtagt ttgttgcgta
      241 gtgtgagcat aaatcatgga tcacataaca actgaaaggg taatgaatat tgaatatcga
      301 gcaaggaata tcgaatgccg aaatttgtca tccctgcgaa cgcagggatc cagaactaaa
      361 acaaacatag attccagcct acgctggaat gacaatcact tcgaaattcc ttgttccttg
      421 ttcattattc gatattctta ttcttccttt cacctttcgc cttcttgccc tgagcttgcc
      481 gaagggttca gctttgagct aatattttta ttccactcca agtctccctt cttccacacg
      541 taataaaatc ccaacaacaa gatcaggatg aaaacaaaga cctcgatcaa agcgatcacc
      601 ccatcgccct tggccaccca ttcccgaaac acgcgcacca cagggaacat gagtgccact
      661 tccacatcga aaatcacaaa cacaagcgcg atcacataga accggatatt gaaattcatc
      721 cacgccgcac caacaggttc ttcaccacat tcgtaggtct tgagtttttc cgggttgggg
      781 ttatggggac gcaacagcga cccaatgaac agtgtcacaa agataaaacc gataccaacg
      841 agagcaaaaa tgatcacatt tgaataatta aaaagcatca tcgcgcagcc cttttagcaa
      901 agccaatggc gaccgacaat caaaaaattt tccttatttt ccacaacaaa aacaaaatga
      961 tctgcgccat gatcaaggcc agaatcgccg tggcccctcc gcgactgaac ccatatgagt
     1021 gcaacccgct ggccagcaca tagttcaccc caaaccacgc gatcacgatc gtcaaaaacg
     1081 cgagcgccac atagggcaga aacgattcat cgccaatcca tcctgcgtat ctaccatgca
     1141 acactgccag ataagccatc aaggctacca gagaccaggt ttccttggga tcccaacccc
     1201 aaaaccgacc ccagctgtaa tcagcccaca cggcccccag caaaatgcca gcagacaaca
     1261 acaccacacc gattctgaga caatcatatg tcatttcatt gaggtgtttt ccttcctcgt
     1321 agccaatttt ggacgcgatg cccttgatca tataataatt ggcgatcagc caactcagcc
     1381 caaaggccgc gtagctgatg gtgatggtgg tcacatgagt ggccagccaa aagttgtcac
     1441 gcaacacagg tgccagcggt ttgagtgtgg ggtccagcat cctgtcggca aacatcatca
     1501 tgaacaaact gaccagattg atcaaaacgc caaacaacaa aaacaaacgt tcccgacgcc
     1561 accaccccag caaaccacca aacaccaagg cccccaaacc aacccacaag acggtctcat
     1621 acatgttggt caccggggcg cgtcctgcaa tgagcacacg aaaaaccacc atagccgctt
     1681 gcagaaaaaa cagaagagac aggacagaca cggtcatggg gtgatgcgga tttttgagaa
     1741 caaatcccaa aacgagtgcc accatcgccg ccagcaaggc catgtgcaac agatgcaaac
     1801 gatgataggt cagctcaacc agataatggg gattttgctg tctgaacgct tgcccgtact
     1861 gttggatcac atcgatcacc gcagaggggt tc
//
LOCUS       DATDUN010000518         1250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_483168, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000518 DATDUN010000000
VERSION     DATDUN010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483168"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VJC18_07235"
     CDS             <1..997
                     /locus_tag="VJC18_07235"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HLD45212.1"
                     /translation="IETEGLADEVFAISAADDDSDPDDDSTADSKAAISLNVHNNTIA
                     LGSLTQGLTAINAGVNLFTNFNGPDGDELFYLENLDVQNNTIAIDTAGNEGAIGINAG
                     LIRQPALIANNVISITGSVSVSVPLLTYLSDIDVVYNTIYTDTVTSQALGWTAFATDS
                     ETESVNYRDKRIGEVANNIFHLRSTSNNSNDPTDCATMMFEEINNTVNASYVTAASPE
                     TFRNNDVFLDSDCQAQYVYGDATDVGTNTPIDTVTQLNAKTGFSASDPTISANNISAD
                     PDFTNLTAGDFTLLNTSPCLDAGLAFDTETWTDLLGTARPLGDAVDMGAYEFLEL"
BASE COUNT          319 a          358 c          294 g          279 t
ORIGIN      
        1 gattgaaacc gaagggctcg cggatgaggt ctttgccatc tctgccgctg atgatgattc
       61 tgatcccgat gacgacagca ctgcagacag caaggccgcc atcagcctca atgtgcacaa
      121 caacaccatc gccctgggca gtttgactca ggggttgacc gcgataaacg caggggtcaa
      181 cctgttcacc aacttcaatg gaccagacgg cgatgagctg ttctatctgg aaaatctcga
      241 tgtccaaaac aacaccatcg ccatagacac ggcaggcaat gaaggcgcga tcggcatcaa
      301 cgcgggactc atccgtcaac cggcactcat cgccaacaat gtgatcagca tcacaggctc
      361 cgtctcggta tcggtccccc tgctgaccta tctgagcgac attgatgtgg tttacaacac
      421 aatctacacg gacactgtca cctcacaggc actgggttgg acagcctttg ccacggacag
      481 tgaaactgaa tccgtaaact acagagataa acgaatcggc gaggtggcca ataatatttt
      541 tcacttgcga tccacgtcaa acaacagcaa tgaccccacc gactgtgcca caatgatgtt
      601 tgaggagatc aacaacactg tcaacgcctc gtacgttacg gccgccagcc cggaaacttt
      661 tcgcaataat gatgtctttt tggattccga ttgtcaggcc caatacgtat atggtgacgc
      721 caccgatgtg ggcaccaaca caccgattga tacggtcacc caactcaacg ccaaaacggg
      781 tttttctgcc agcgacccca caatcagcgc caacaacatc agcgccgatc ccgattttac
      841 caacctgacc gctggggatt ttacactgct gaacacctcg ccctgccttg atgctggtct
      901 ggcctttgac accgaaacgt ggacagacct tctcggtacc gcacgacccc tgggtgatgc
      961 cgttgacatg ggggcatacg aatttctgga attgtgacaa acagtcatgc agtgattggc
     1021 gccatacgtc agacaccaaa aatcccttgc tcttttgcaa catttcccgc aaagttttgc
     1081 gcatgatcac aaaaaaacga actcaccaca aggtgtttct gggcatgatt gtaggtgtgc
     1141 tggcgggttt ggccatgcaa tattctggtc tgtctgatca aaccatcgcc acgctggtgc
     1201 gcctcgtcaa accggtgggt gatatctttt tgcgcctgat cttcatgatg
//
LOCUS       DATDUN010000519         5929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_230786, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000519 DATDUN010000000
VERSION     DATDUN010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230786"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VJC18_07240"
     CDS             complement(<1..660)
                     /locus_tag="VJC18_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HLD45213.1"
                     /translation="MKTITVGKRKIGEGENLYFIADIAANHDGDLQRALKLIELAKEA
                     GADAAKFQNFVARKIVSRNGFESLGGQLSHQAKWKKPVYEVYQDASLPREWTAKLKHK
                     CDEVGIEYFTSPYDFESVDQVGPFVSVYKIGSGDITWLEIIEYIAKKGKPVMLATGAS
                     MLEDVDRAMTCLMQFTKDVVLMQCNTNYTATPENFKHINLRVLQTYSQKYPEVILGLS
                     DH"
     gene            complement(657..1556)
                     /locus_tag="VJC18_07245"
     CDS             complement(657..1556)
                     /locus_tag="VJC18_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLD45214.1"
                     /translation="MERVKRILITGSAGMLANAVGPILQEDLNYEVYACDRLQTLWAD
                     ATHQKHFDLTDSDQLKMYLDRIEPDIIIHCLALVDLEYCETHPQVAGDVHEGITYTLA
                     HHCPQTSFVYISTDSVFDGEQGNYSETDEPKPLNVYARTKLAGEQVVLQESRKPLVLR
                     TNIYGFKNPPGGSLAEWALTHLLKGESIGGFTDVMFNALYIRQLAGTIKQLLSSDCRG
                     VLHVGSQDHFSKYDFLIKLARECMRPTSLIRSTTSEHMLFKAKRPRDTTLDVSKFTSL
                     VTSVPVIEEGIKALVEDYRKVFA"
     gene            complement(1564..2874)
                     /locus_tag="VJC18_07250"
     CDS             complement(1564..2874)
                     /locus_tag="VJC18_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLD45215.1"
                     /translation="MLSGTQLYQRAKILIPGGTQLLSKRPEMLLPEYWPAYFSKAKGC
                     QIWDLEGVEYTDMAYMGVGSNILGYADEDVDAQVKSAIDHGNISTLNAPEEVYLAEVL
                     LQIHPWAHMVRYAKTGGEAMAIAVRIARASSNRDVVLFCGYHGWHDWYLSANLAEEKS
                     LDGHLLPGLQPRGVPRQLLGTSFPFKYNDTEAFNRLIAKHATKVGAIVLETIRNEEPK
                     SEFLSAIQRTCQKYQIPFIVDEISAGWRLTLGGAHQKYGIEPDIAVFAKAMSNGYPMS
                     AIIGKKHVMSSAQDSFISSTYWTDRIGLVASLACIEKMRAQHVAEYVVRSGQQVKEIW
                     TKTAKDRGVAIHVGGIDPIAHFSFQHDQPLVLKTLFTQGMLDRNILATTAYYASFAHK
                     NSHIDLYEKAVREVFGQVREAIQTGNPETFLKGPVCHSGFSRLT"
     gene            complement(2886..3647)
                     /locus_tag="VJC18_07255"
     CDS             complement(2886..3647)
                     /locus_tag="VJC18_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HLD45216.1"
                     /translation="MIIAIIQARVLSTRLPRKVLMSLCGKTVLEHVVSRIQESRFIDT
                     VVVATTTRPEDDAIVDLCQRAGMAMSRGSEDDVLDRYYRTAQPYRPDHVVRVTSDCPL
                     IDSEIVDQVIEAHLSSGADYTSNTLQETYPDGLDVEVFTFSSLEKAWKEASLKSEREH
                     VTPYIKKNQQMFKLNSVVNKTDLSQKRWTLDQPEDWILIKAVYDHLYEKNTKFCLADI
                     LHYLSQNPEVEQCNQIIRRNEGYEKSLQNDFVVTK"
     gene            complement(3775..4707)
                     /locus_tag="VJC18_07260"
     CDS             complement(3775..4707)
                     /locus_tag="VJC18_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLD45217.1"
                     /translation="MRPSSKIALGTVQFGMNYGVNNKRGQIPAIEVSQILCEASQHGI
                     TCLDTAQGYGNSEEVIGRAAREFSLDFDVVTKLPTHMVTPVEACVAESQKRLGLHKLY
                     GCLVHRFDLITQGRDVWDGLRRVRREGLVAKIGCSLYYLHELDYLFSHVTDCDLIQVP
                     YNVFDQRFEPYFAEAKKRGMEIHVRSVFLQGMVFKKPEELAPQFAPMKKHLLALQHIA
                     SDSGLSLTALCLNFVCLNPDVDRVIVGVDGLDHLRANMAALLCSSAVNGFSAQLGELA
                     ISDENMVVPSLWNKGTSNNHVRYEKLQSESKQGR"
     gene            complement(4714..5709)
                     /gene="pseB"
                     /locus_tag="VJC18_07265"
     CDS             complement(4714..5709)
                     /gene="pseB"
                     /locus_tag="VJC18_07265"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HLD45218.1"
                     /translation="MDNKVVLITGGTGSFGKKFVERILQKYNPSKVIIYSRDEHKQYL
                     MHKQFIQHEDKLRFFIGDVRDRERLYRAFDGVDIIIHAAALKHVPIIEYNPLEAIKTN
                     VIGAANIIDAAIDCGVEKVFALSTDKAVNPINLYGASKLCSDKLFVAGNAYSGKRQTQ
                     FSVVRYGNVAGSRGSVIPYFSSLIKQEKGSLPITDSRMTRFWITLDEGVDLVFKAMAT
                     ARGGEVYVAKIPSFRVVDLATAMSPQSKQEQIGIREGEKLHELMITKEDARTTYEYDK
                     HYVIYPNFDWWDIEKHLIPGGKKVADHFEYSSDTNPQFLTVQELQSYLNKIDIIY"
     gene            complement(5702..>5929)
                     /locus_tag="VJC18_07270"
     CDS             complement(5702..>5929)
                     /locus_tag="VJC18_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45219.1"
                     /translation="ENAIISALDNLSHKKTMIIVAHRLTTVQHSDVIYFLEAGKILES
                     GAYHELIARNTKFELMTKMNQLSRTKEAING"
BASE COUNT         1631 a         1463 c         1139 g         1696 t
ORIGIN      
        1 atgatcagaa agacccaata tcacttctgg atatttttga ctgtaggttt ggagaactct
       61 taaattaatg tgcttaaaat tttctggggt agccgtataa ttcgtattgc actgcatgag
      121 cacaacgtcc tttgtgaact gcatcagaca agtcatcgcc cgatccacat cttccagcat
      181 ggacgctccc gtagcaagca tgacaggctt accttttttg gcaatgtact caataatctc
      241 aagccaagta atgtcgcctg aacctatttt gtaaacggat acaaaagggc cgacttgatc
      301 aactgattca aaatcatagg gacttgtaaa atattctatc cccacttcat cgcatttatg
      361 ttttaatttt gctgtccatt cccggggtag actcgcgtct tgatacacct cataaactgg
      421 ctttttccat tttgcttgat gagataattg accacccaat gactcaaaac cattccgact
      481 cacaattttt ctcgcaacaa aattttgaaa tttggccgca tcagccccag cttctttggc
      541 caattcaatc aatttgagtg cccgttgcaa atcaccatca tggttcgccg caatatcggc
      601 aataaaatac agattttctc cttctccaat ttttcttttg cctacagtga tcgttttcat
      661 gcaaatacct tcctgtaatc ctcaacaagc gccttgatcc cttcctcaat aacgggcact
      721 gaggtcacaa gagacgtgaa tttgctaaca tcgagcgtgg tatcacgagg cctttttgcc
      781 ttaaacagca tgtgttcact ggtggtcgaa cgaataagag atgttggcct catgcactca
      841 cgggccaact tgatcagaaa atcgtacttg ctaaaatgat cctgactacc cacatgcaaa
      901 actccacgac aatcagaaga caataactgt ttaatggtcc cagccaactg cctaatgtat
      961 agggcattaa acatcacatc agtgaaccct ccaatggatt ccccttttaa taaatgcgtc
     1021 aaagcccatt ccgccaagga tcctccggga gggtttttga aaccataaat gtttgttctt
     1081 aagaccagtg gttttcgtga ttcctgcaaa accacttgct cccctgccaa cttggtgcgg
     1141 gcgtaaacat tgagaggctt tggctcatca gtttcagaat aattaccctg ctccccatca
     1201 aacacggaat ctgtggagat gtaaacaaag cttgtttggg gacaatgatg cgccaacgta
     1261 tatgttattc cctcatggac atcaccagca acctgcgggt gtgtttcgca atattcaaga
     1321 tcaaccaaag ccaaacagtg gatgatgatg tccggttcta ttcgatccaa atacattttt
     1381 agctggtcgc tgtctgtgag atcaaaatgt ttttgatggg tcgcgtctgc ccacaacgtc
     1441 tgcaggcgat cacacgcata gacttcatag ttcaaatctt cctgtaatat gggcccaacc
     1501 gcgttggcca gcatcccggc agaacccgtg atcaaaatgc gttttacacg ttccatatac
     1561 ctttcatgtc aaacgactaa atcctgaatg acaaacaggg cccttcaaaa aagtctctgg
     1621 gtttccggtt tggatagctt ctctgacttg cccaaaaacc tctctcacag ccttttcgta
     1681 caaatcaata tgactgtttt tgtgagcaaa cgatgcatag taagcggttg tcgccaaaat
     1741 atttctatcg agcatccctt gggtaaacaa tgttttcaaa acaagtggct ggtcatgctg
     1801 aaacgaaaag tgtgcgatag gatcaatccc ccccacatga atcgccacgc ctcggtcttt
     1861 tgctgtcttg gtccaaattt cttttacctg ctgcccggat ctgaccacat attctgcaac
     1921 atgctgtgca cgcatttttt caatgcatgc caagctggcc accaacccaa tgcgatccgt
     1981 ccaataggtg ctactgataa agctatcttg tgcagaactc atcacatgtt ttttgccaat
     2041 gatggcagac atgggatacc cattgctcat cgccttggca aagactgcta tgtctggttc
     2101 tattccatat ttttgatgag ctccaccaag tgtcaatcgc cagccagcac taatctcatc
     2161 aacgataaaa gggatttggt atttttgaca ggtcctctga atggcagaaa gaaattcgga
     2221 ttttggttct tcattgcgaa tggtttcgag cacaatagca cctactttgg tggcatgctt
     2281 tgcgatcagg cgattaaacg cctcagtatc attgtatttg aagggaaatg aggtgcccaa
     2341 gagttgacgt ggaacccctc tgggttgcaa gccgggcaac aagtggccat ccagtgattt
     2401 ttcttctgcc aaattggccg acaaatacca atcatgccat ccatgatacc cacaaaataa
     2461 aactacatcc ctatttgatg acgcacgggc aatacgcacc gcaatggcca tggcttcacc
     2521 acccgttttg gcatacctga ccatatgggc ccatgggtgt atttgcaaca acacctccgc
     2581 caaataaact tcttccgggg cattgagggt tgaaatattt ccatgatcaa tggccgattt
     2641 aacctgcgca tcaacatcct catcagcata acccagaatg ttagacccca cccccatgta
     2701 cgccatgtcg gtgtactcca ccccttccaa atcccaaatc tgacaacctt ttgccttgga
     2761 aaaataagct ggccagtatt caggcaacag catttcaggt ctcttggaaa gtaactgggt
     2821 ccctcccggt atcaaaattt ttgctctctg gtatagttgt gttcctgaca acataaattt
     2881 ccctattatt ttgttaccac aaaatcattc tggagtgatt tctcataccc ctcattgcgc
     2941 ctgatgatct gattgcattg ttccacctca gggttttgtg acagataatg caagatatcg
     3001 gccaaacaaa attttgtatt tttctcgtag agatgatcat agacagcctt gatcagaatc
     3061 caatcctccg gctgatctaa ggtccacctt ttttgtgaca aatcagtctt gttgaccaca
     3121 gaattgagtt tgaacatctg ttggtttttt ttgatgtacg gggtaacgtg ctctctttct
     3181 gatttgagcg aggcttcttt ccaagccttt tccaaggatg aaaaagtaaa cacctcaaca
     3241 tcaagcccat cgggataagt ttcctgtaat gtatttgatg tgtaatcagc acccgaactc
     3301 aaatgggctt caatcacctg atcaacgatc tcactgtcaa tcaatggaca atctgatgtc
     3361 actctcacca catgatccgg ccgatagggt tgagcagtgc gatagtagcg atcaagcaca
     3421 tcatcttccg aaccacgaga cattgccata ccggccctct ggcataaatc cacaatggca
     3481 tcatcttctg gacgggtcgt cgtggcaaca accacagtat caataaagcg actttcctgg
     3541 atgcgtgaca cgacatgttc caaaactgtt ttgccacata aggacatgag aaccttgcgg
     3601 ggcaatcttg tggaaagaac tcgtgcctga atgatggcaa tgatcatcaa ttctccttat
     3661 gggcacctct aataatcatc ttctactgca aaattacact ctggtctgca actgcgtccg
     3721 atggcacaaa aagtcctcaa cgtggcgtgc cttacacgtc tgcgggcttt ttgcttatcg
     3781 tccttgtttc gactcagatt gtaatttttc gtaacgaaca tggttattag aggtgccctt
     3841 attccataat gagggaacca ccatgttttc atcagaaatg gccaattctc ccaattgcgc
     3901 cgagaatcca ttgactgcag acgaacacaa aagtgcagcc atatttgctc gcaaatgatc
     3961 caagccatcc acacccacta tcacacgatc aacatcagga ttcagacaaa caaaattcaa
     4021 acataatgct gtcaaagaaa gaccggagtc agacgctatg tgttgtaatg ccaagaggtg
     4081 ctttttcata ggcgcaaatt gtggggccaa ttcctcaggc tttttaaaaa ccatgccctg
     4141 caaaaaaact gatctcacat gaatttccat cccccgtttt tttgcctctg caaaataggg
     4201 ttcaaagcgt tggtcaaata cattataggg gacctgaatc aaatcacaat ccgtgacgtg
     4261 gctaaacaga tagtcgagct catgaagata ataaagcgaa catcctattt tggcgacaag
     4321 cccctctcgc cgaacgcgtc gcaggccatc ccaaacatct cgaccctgtg tgatcaaatc
     4381 aaaacgatga accaaacaac catatagctt atgcaaaccc aatctttttt gcgattccgc
     4441 gacacaggcc tcaacaggtg taaccatgtg cgttggcaat ttggtgacca catcaaaatc
     4501 cagggagaac tcacgagcag cgcgaccaat gacctcctcg ctgttcccat aaccttgggc
     4561 agtatcaaga catgtgattc cgtgttggga tgcttcacac aatatttgag atacctcaat
     4621 ggcagggatc tgcccgcgtt tgttgttcac accatagttc attccaaact ggactgttcc
     4681 caatgctatt ttagaagagg gcctcacgct gtctcaataa atgatatcga tcttgttcag
     4741 ataactctgc aattcttgta ctgtaagaaa ctggggattg gtatcagatg aatattcaaa
     4801 atggtcggcc acttttttgc caccaggaat caaatgcttt tcaatgtccc accaatcaaa
     4861 atttggataa atgacgtaat gtttgtcata ttcataggtt gttctagcat cctctttagt
     4921 gatcatcaat tcatgcaatt tttctccctc tctgatgcct atctgttcct gtttggattg
     4981 cggactcatc gccgttgcca gatcaacaac gcggaatgaa ggaattttgg ccacatacac
     5041 ttccccaccc cttgctgtag ccatggcttt gaacaccaaa tcaacacctt catcaagagt
     5101 gatccaaaaa cgagtcatcc gagaatctgt gatgggcaaa ctgccttttt cttgtttgat
     5161 aagggaggaa aaataaggaa tgaccgagcc tctgcttcca gctacattgc catacctcac
     5221 gacactgaat tgggtttgcc ttttgccgga ataggcattt cctgcaacga acagtttgtc
     5281 agaacagagt ttggatgctc catacagatt aatgggattc acagccttgt cggttgacag
     5341 ggcaaagacc ttctccaccc cacaatcgat cgccgcatca atgatattcg ctgcgccaat
     5401 cacattagtc tttattgctt cgagggggtt atattcaata atcggtacgt gttttagggc
     5461 cgcggcatgt ataataatat ctacgccatc aaaagcccga taaagtcttt ctctatctct
     5521 tacatcacct ataaaaaacc ttaatttatc ttcgtgttgt ataaattgct tatgcatcaa
     5581 atactgttta tgctcatccc gagaataaat aataactttg ctaggattgt acttttgtaa
     5641 tattctttca acgaattttt ttccaaagga tcctgtccca cctgttatca gaacaacttt
     5701 attatccatt gattgcctcc ttagtacggg acagttggtt catcttcgtc atcagctcaa
     5761 attttgtatt ccttgctatc aattcatgat aagcaccact ctctaatatt ttcccagctt
     5821 ccaagaaata gatcacatca ctatgctgaa cagtcgtgag tctgtgcgcg acaataatca
     5881 tcgttttttt gtgtgacaga ttgtcaagcg cgcttataat ggcattttc
//
LOCUS       DATDUN010000520         5561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_483188, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000520 DATDUN010000000
VERSION     DATDUN010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483188"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /gene="secY"
                     /locus_tag="VJC18_07275"
     CDS             complement(<1..994)
                     /gene="secY"
                     /locus_tag="VJC18_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HLD45220.1"
                     /translation="MANPIFNLTKMPDVRKRLLFSMAMLAVYRLGVFIPTPGINVAKL
                     KLFFSGQGGQTIFELINLFSGGALEQFSIFALGIMPYISASIIFQLLTVSLPALKRLQ
                     EEGEAGRNKINQWTRYGTVALSLVQGFFISYGLLQNGYVNADSSGAGFLFTTMISLAA
                     GTCFIMWLGEQITERGIGNGISLIIFSGIVARLPGGFRDAWVNRDAYFGVFGFVLLLG
                     FLFLIIATIIFFERCQRRVPIHFAKRVVGNKMYGGQSSHLPLKINMAGVIPPIFASSI
                     LLFPATIASFSHSPKLQALAGFFTTGWFYTTMYIAAIIFFTYFYTAVTFNPNDVA"
     gene            complement(1034..1477)
                     /gene="rplO"
                     /locus_tag="VJC18_07280"
     CDS             complement(1034..1477)
                     /gene="rplO"
                     /locus_tag="VJC18_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HLD45221.1"
                     /translation="MLLNQLRAPKGASKNRKRLGRGESSGLGKTSGRGHKGQKSRSGG
                     YHKVGFEGGQMPLKRRLPKQGFHNIYRKEYAIVNVADLSRYQVNAEIDMAAVVGTKGK
                     SKRLEGLKILGKGNIEIALTVKAHKFSKTAVEKIEKAGGKVVVLS"
     gene            complement(1587..1766)
                     /gene="rpmD"
                     /locus_tag="VJC18_07285"
     CDS             complement(1587..1766)
                     /gene="rpmD"
                     /locus_tag="VJC18_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HLD45222.1"
                     /translation="MEKKIKIKYVHSPIGRKQDQKDTVRCFGFTRLNQVKEFPDTPCI
                     RGMVNKVPHLVQIVE"
     gene            complement(1778..2224)
                     /gene="rpsE"
                     /locus_tag="VJC18_07290"
     CDS             complement(1778..2224)
                     /gene="rpsE"
                     /locus_tag="VJC18_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HLD45223.1"
                     /translation="MDRVVHISRVAKVVKGGRRFSFSALVVCGNGNGEVGFGLGKANE
                     VPDAIRKGAEKARRNMIQVPLYQRTIPHDVIGRYGAGRVFMKKASAGTGVIAGGAVRA
                     VFESIGVQDILTKCIGTSNPHNVVRATFEGLKQFKNTEGNRAQTTS"
     gene            complement(2495..2839)
                     /gene="rplR"
                     /locus_tag="VJC18_07295"
     CDS             complement(2495..2839)
                     /gene="rplR"
                     /locus_tag="VJC18_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HLD45224.1"
                     /translation="MATKKLTPRQKRQVRIRKKISGAPEKPRLTVFRSNRYLYVQLID
                     DLNQQTLASASTIKEKSGCNKSAAANLGKEIAEKAVARKISTICFDRSGYRYHGVLKE
                     IAEAARKAGLQF"
     gene            complement(2841..3383)
                     /gene="rplF"
                     /locus_tag="VJC18_07300"
     CDS             complement(2841..3383)
                     /gene="rplF"
                     /locus_tag="VJC18_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HLD45225.1"
                     /translation="MSRTGKQPILVPDKVKVSLNSNLFMAEGPLGKETVEVNLLAKVA
                     IDDNKIVVTRVNNTQHARCVHGMTRALLANAVKGVHKGFQKDLEINGIGYRAESKGKT
                     LKLTLGFSHAIDFPVPEGIKVETPEPSKITVKGANKELVGRFTSQVRELRPPEPYKGK
                     GIKYAGEHIIRKVGKAAGGK"
     gene            complement(3464..3865)
                     /gene="rpsH"
                     /locus_tag="VJC18_07305"
     CDS             complement(3464..3865)
                     /gene="rpsH"
                     /locus_tag="VJC18_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HLD45226.1"
                     /translation="MATTDPIADLLTRIRNAQHARKDTCEIPASQLKESVLKILQQTG
                     WVESYTRQEQAPQDVLVVRLKYKGKLRTPTINHVRRISKPGRRIYRRYQEIKPLLDGY
                     GFAILSTSKGVLKDDDARRQKVGGEVLCEIW"
     gene            complement(3919..4104)
                     /locus_tag="VJC18_07310"
     CDS             complement(3919..4104)
                     /locus_tag="VJC18_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HLD45227.1"
                     /translation="MAKKSMIAKANRKPKFSSRKVYRCPLCGRDQGFLRKFQMCRLCF
                     RKRALNGELPGVKKASW"
     gene            complement(4106..4642)
                     /gene="rplE"
                     /locus_tag="VJC18_07315"
     CDS             complement(4106..4642)
                     /gene="rplE"
                     /locus_tag="VJC18_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HLD45228.1"
                     /translation="MLLHEKYQKEIVPLLKEKLGITNKMLVPKLKKIVINTSTGEALL
                     NAKILEQLALELAAITGQRPVITKSKKAIANFKLRQGMPLGVCVTLRGVRMYEFFNRL
                     VNLALPRTRDFKGLSPKGFDGRGNYTLGISEQTIFPEIVPEKVEKPRGMNITIVTSSD
                     SDVQARELLTVMGFPFRS"
     gene            complement(4721..5089)
                     /gene="rplN"
                     /locus_tag="VJC18_07320"
     CDS             complement(4721..5089)
                     /gene="rplN"
                     /locus_tag="VJC18_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HLD45229.1"
                     /translation="MIQQNTVLNSADNSGAKKLYCIKVLGGSGRKYATIGDVIVVSVK
                     EALPNAKVKKGDIKKGVVVRIKKEVRRKDGTYIRFDENSAVLIEPNGEPIGTRIFGPV
                     ARELRAKKFMKIISLAPEVL"
     gene            complement(5161..5415)
                     /gene="rpsQ"
                     /locus_tag="VJC18_07325"
     CDS             complement(5161..5415)
                     /gene="rpsQ"
                     /locus_tag="VJC18_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010646396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HLD45230.1"
                     /translation="MNKNKKTLEGIVIRNKMIKTVIVEIKSQFKHPVVHKIVTTTKKF
                     KAHDQNSECGLGDRVVIRECKPISKEKRWVVAKILEKAAS"
BASE COUNT         1369 a         1533 c         1112 g         1547 t
ORIGIN      
        1 cagcaacatc gtttggattg aaggtcacag ccgtgtagaa ataagtgaag aatatgatcg
       61 ccgcaatata catggttgtg taaaaccaac ccgttgtgaa aaaacctgcc aaggcctgca
      121 gtttcggcga atgggaaaaa ctggcaatgg tggcaggaaa cagtaaaatc gacgaggcga
      181 agataggcgg gatcacgcca gccatattga tcttgagcgg caaatgcgat gactgtcccc
      241 catacatctt attgccaaca acccttttgg caaaatgaat gggcacgcgt cgttgacacc
      301 tttcgaaaaa aatgatcgtg gcgatgatca aaaacaaaaa acccaacaac agcacaaaac
      361 caaagacccc aaaataggcg tcacggttca cccacgcatc acgaaaacca ccaggcaacc
      421 tggccacgat gcccgaaaaa atgatcaggg agattccgtt accgatcccg cgctctgtga
      481 tctgttctcc caaccacatg atgaaacacg tccctgcggc caaactgatc atggtcgtaa
      541 acaaaaaacc agctccggat gaatcggcat tgacgtaccc attttgcaac aacccgtagc
      601 taatgaaaaa accctgaacc agcgacaaag caacagttcc ataacgagtc cactggttga
      661 tcttgttacg tcctgcctca ccctcttcct gcaacctctt caaggctggc agtgaaacag
      721 tcagcaactg aaagatgatc gacgcgctga tgtacggcat gatccccaat gcgaaaatgg
      781 agaactgttc caaagcgcca cccgaaaaca aattgatgag ttcaaagatc gtctgtccac
      841 cctgtcccga gaaaaataac ttcagcttgg ccacgttgat tccgggagtg ggaataaata
      901 cgcccaggcg atacaccgcc aacattgcca tagagaacag caaacgcttt ctcacgtctg
      961 gcattttggt tagattgaaa attggattgg ccaaaacaat tctccttgta aaagtttaca
     1021 ttcacttccc gtttcaactc aaaacaacaa ccttgccacc tgccttctcg attttttcca
     1081 cagctgtttt cgaaaattta tgggctttga ccgtcaaagc tatttcaatg ttgcctttgc
     1141 caagaatctt caggccttcc agtcgtttcg attttccctt ggtgcccacg accgctgcca
     1201 tatcaatttc tgcgtttacc tgataacggc taaggtcagc gacattcaca atagcgtact
     1261 ctttccgata aatgttgtga aacccctgtt tgggcaaacg tctcttcaaa ggcatctgac
     1321 caccttcgaa tcccactttg tgatacccgc cagaacgcga tttctggcct ttatggcctc
     1381 gaccgctcgt cttgccaagg ccagaagatt caccacgacc taaccgcttt ctgttttttg
     1441 acgccccttt gggagctcgt aattgattca ataacataat ccaccttctc cagattttta
     1501 ccgtcacacc ccccaagccc cctcgccacg cctgtggacg agtcactcag gccaaatgac
     1561 agcaaccatt tgttcgttcg tcgctttcac tccacgattt gtaccaaatg aggaacctta
     1621 ttgaccatac cacgaataca cggcgtatcg ggaaactcct tgacctgatt cagtcgcgta
     1681 aacccaaaac acctcacggt atccttttga tcctgcttac gtccgatggg actgtgcaca
     1741 tactttattt tgattttctt ttccataatt tcactcatca gctagttgtt tgcgcccgat
     1801 tgccctctgt gtttttaaac tgcttcaaac cttcaaaagt ggcccggaca acattatgag
     1861 gattgctggt gccaatgcat tttgtgagga tatcctgcac accgattgac tcaaacacag
     1921 ctctcacagc accaccggcg atcacacccg taccagcgct ggccttcttc atgaataccc
     1981 gaccggcacc ataacgacct atgacatcgt gtgggatcgt tcgttgataa agaggtacct
     2041 ggatcatgtt gcgtcgcgct ttttcagccc ctttgcgaat ggcatcgggc acttcgttgg
     2101 ctttgcccaa accgaaacca acttcaccat ttccgttccc acaaaccacc aaagcggaaa
     2161 agctgaaacg acgtccgccc ttcacgacct tggccacgcg actgatgtgt accacgcgat
     2221 ccacaaactt agactcgttg ggatccccaa aataatctga gggctcgtca cgagtcgtcg
     2281 taaccacctc tgtctccacc acgtcagcgg tcacagtttt tgcgacctct tcggacacga
     2341 cgacctgagg ttcaccggat tcctgttttt gagcgttctc tttcatttcc tgttccttta
     2401 tttttgtcac aaagcctcca cttgggctgt cagccatcag ctatcagtcg caaggaagtg
     2461 acccctgact gacgaccaaa gacttaaagg ctgcctagaa ttgcagacct gccttcctgg
     2521 cagcttcagc aatttcttta agcacaccat ggtagcggta accactgcga tcaaagcaga
     2581 tggtgctgat ctttcgcgcc acagcctttt cggctatttc tttaccaaga ttggcggcgg
     2641 cggatttatt gcaaccactc ttttctttga tggtgctcgc agaagcaagt gtctgttggt
     2701 tcagatcatc aatcaattgt acatacaaat aacgattgct tctgaaaacg gttaatcttg
     2761 gtttctcggg cgcgccagaa attttttttc tgatacgaac ctgtctcttt tgtctgggtg
     2821 ttaatttttt cgtcgccatg ttattttcct ccggcagctt taccaacttt tctgatgatg
     2881 tgttcacctg cgtattttat tcccttgcct ttataaggtt ccggcggtct caattcacgt
     2941 acctgcgacg tgaagcgccc caccaattct ttattagcac ctttcacagt gatcttgctg
     3001 ggttccggcg tttcgacctt gatcccttcc gggacgggaa aatcaatcgc gtgagagaaa
     3061 cccagtgtga gtttcagcgt ttttcccttg gattccgcgc ggtacccaat accgttgatc
     3121 tcgagatctt tttgaaatcc tttgtgtacc cccttaacgg cattcgccag aagcgctcgg
     3181 gtcattccat gcacacaccg tgcgtgttgt gtgttgttca ctcgggtcac gacgatctta
     3241 ttgtcatcga tagcaacctt tgccaataaa ttcacttcga ccgtctcttt ccccaatggt
     3301 ccctccgcca taaacaagtt actgttgagg ctcaccttga ctttatcggg aacaagaatg
     3361 ggctgttttc cagttcgtga cataacgcat cctaacccgg cagtgccgga aaatcgaaat
     3421 ccaatattct aaatccaaaa cacaaccatc gtggccaaca gtgttaccaa atttcgcaca
     3481 acacttcgcc gccaactttt tgtcttctgg cgtcgtcatc cttcaagaca cccttcgatg
     3541 ttgacaggat cgcaaaacca tacccgtcca gcaaaggctt gatttcttga tagcgacgat
     3601 aaatacggcg acctggtttg ctgattctcc gcacgtgatt gatggtcggt gtcctcagct
     3661 tgcctttgta tttcaacctg acaaccaaga catcctgcgg tgcctgctct tgtctggtat
     3721 aactctccac ccatcccgtc tgctgtaaaa tcttcaacac actctctttg agctgcgacg
     3781 cagggatctc acaagtgtcc ttgcgagcgt gttgcgcatt gcgaattctc gtcaataaat
     3841 ctgcaatggg atctgttgtt gccatagttt ctttctttct gtaaacctgc ctccctgcct
     3901 gctggcagac gatgcaactt accaactagc ctttttaaca ccgggcaatt caccattcag
     3961 tgcgcgtttg cgaaaacaaa ggcgacacat ttgaaacttg cgaagaaagc cctgatctcg
     4021 tccgcacaag ggacaacgat aaacttttcg tgaggaaaac tttggttttc tgtttgcctt
     4081 ggcgatcatt gattttttag ccatatcaac tcctaaatgg aaatcccata accgttaaca
     4141 attcccttgc ctgaacatcg ctgtccgacg aggtcacgat ggtgatgttc atccccctgg
     4201 gtttttcaac tttttccgga acgatttctg gaaaaatcgt ctgttcagaa attcccagtg
     4261 tataatttcc gcgaccgtca aaacccttcg gtgacagccc tttaaagtca cgtgtgcggg
     4321 ggagggccag attgaccaga cggttgaaaa actcatacat gcgcacccct ctgagggtga
     4381 cacacacacc caacggcatg ccttgtctga gcttaaagtt cgcaatggcc tttttggact
     4441 ttgtaatcac cggtctctgt cccgtgatgg ctgccaactc aagcgccaat tgctccagaa
     4501 tcttggcatt caataaagcc tcgcccgttg acgtgttgat cacaatcttc ttgagcttgg
     4561 gcacaagcat tttgttagtg atccccagct tctccttgag caacgggaca atctcttttt
     4621 gatatttttc atgcaataac ataatccact ctcgtttaca tgttccccag ctacaggcct
     4681 aaattcgctt gctcgccggc aaacaataaa gttattccct ttacaaaacc tccggtgcca
     4741 acgaaatgat tttcataaat ttcttggcac gcaattccct ggccacagga ccaaaaatac
     4801 gggttccaat gggctcacca ttcggctcaa tcaggacggc tgaattttcg tcgaaccgta
     4861 tgtaggtccc atccttgcgc cgaacctctt ttttgatacg caccacaacg ccttttttga
     4921 tatcaccctt tttcactttg gcgtttggca atgcttcctt gacagacacc acgatcacat
     4981 caccaatggt agcgtatttt ctgccagacc ccccaagaac cttgatacaa taaagcttct
     5041 tggctccgga attatccgcc gagttcaata ctgtattttg ctgaatcata acactttacc
     5101 tcgaaataca gaggagagtc cttgcgtgaa caaaaacgct tccctgcgaa tgaatccttc
     5161 tcatgaagcc gctttctcca gaattttcgc gaccacccac ctcttttcct tgctgatggg
     5221 tttgcactca cgaatgacaa ccctatctcc caacccgcac tcgctatttt ggtcatgtgc
     5281 cttgaacttt ttggtggtcg tcacaatttt gtgaaccacg gggtgtttga actggctctt
     5341 gatttccaca atgactgtct tgatcatctt gttgcggatc acgatgcctt ccaatgtctt
     5401 tttgtttttg ttcataatca aatcccttat gatggtttct tccctttttt gagttcggtc
     5461 agttttgtca tcactcgcgc aacgtccttt ttggtctgac aaatcttgct tttgtcctcc
     5521 aactgttgcg ttttgtgttt cagccgcaat cgaaacaagt c
//
LOCUS       DATDUN010000521         3866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_230794, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000521 DATDUN010000000
VERSION     DATDUN010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230794"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VJC18_07330"
     CDS             <1..738
                     /locus_tag="VJC18_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLD45231.1"
                     /translation="RGLESHCARMKLFGEHVDGTHAESISVPERNVLLMDDSISFEEA
                     AAFPLVFLTAWHMLVYNAQVKPGEVVLVVGAGSGVGSAAIQIAKLHGAEVIATCGGTV
                     KMGQAIALGADHVIDHHQEDIAKRVREITHKKGVDIVFEHVGVKVWESCLRSLAMNGR
                     LVTCGATTGPLVNMDLRHVFIKQQKIIGSTMGSLSEMLLINKFMAQKKLKPVISSVFP
                     FTEVAQAQRYFEESRHFGKVILSWHKA"
     gene            861..2450
                     /locus_tag="VJC18_07335"
     CDS             861..2450
                     /locus_tag="VJC18_07335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLD45232.1"
                     /translation="MLQYLKKIVGVKIALFVTGTLLFVFSILLFVANPFLDRLASGQG
                     GQTVFLLLTLFFICLQVVLIFLAIGLFIGRPLQKIMASMRRIAQGQTEEICDVGEGDE
                     FGELSGNLNKMMQNIRHMLDSRQKIEKRLVAAEEGLKYKSALEDKAKIIERMNLELSG
                     ALSDISLLYTVSQHLSSVLAMDDLLQKVRLIFENKLSCDGFVLLLVDNPARQLKLVAS
                     KNLKDLSEEQKSVDVGDGLAGFVVQKNRSHYVSDLLSFERSRLSVIESDMQGSVFCVP
                     LRVREEVVGVLLVWRQQIDSFTPTDRQSLESIGSQIAVAFDRSLLHTKTKELSVRDEL
                     TDVYNRRYFYQMLHREFERAKRFNRPLSLIMIDLDHFKRLNDTFGHIVGDGVLKKFAQ
                     LIRDHLREIDVLARFGGEEFVVLLSDTAMNNAVIVAEKLRSLVRVQSWEPLLSGGDDR
                     GDMMITISVGVAGFPETAKTQEELVHRADIALYQAKKMGRDRVCVFQPDQDALMSSDL
                     GACEELLSDNVFPIDRAKLSD"
     gene            2572..2967
                     /locus_tag="VJC18_07340"
     CDS             2572..2967
                     /locus_tag="VJC18_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45233.1"
                     /translation="MNFKLLLDEAINRGEKIKQEVLAEVLKSKTIQEIVSNKNFVKAL
                     TKIIETKQEIKHVLDKQTKSILALMEIPSRKEITDLLRQLVKIEGLINRVTHKSTVAK
                     THKSVRAQRVRTTKPRSAAKKRALKKRKK"
     gene            3580..3652
                     /locus_tag="VJC18_07345"
     tRNA            3580..3652
                     /locus_tag="VJC18_07345"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3613..3615,aa:Thr,seq:tgt)
BASE COUNT         1017 a          765 c         1023 g         1061 t
ORIGIN      
        1 agggggctgg agagtcattg tgcccggatg aagctttttg gtgaacacgt cgatgggacg
       61 catgctgaat ccatcagcgt gcctgaacgc aatgtgttgc ttatggatga ctcgatttct
      121 ttcgaagagg cggcggcctt cccgctggta tttctgacgg cttggcacat gctggtctac
      181 aacgcacagg tcaaaccggg cgaggttgtg ctggttgtgg gagccggtag cggagtgggc
      241 agtgcggcga ttcaaattgc caaactgcat ggggcggagg tgattgcaac ctgtggcggt
      301 actgtcaaaa tgggtcaggc aatcgcgctg ggtgctgatc atgtgatcga tcatcaccag
      361 gaagacattg ccaaacgtgt gagagaaatc acgcataaaa agggtgtgga cattgtattt
      421 gagcatgttg gtgtcaaggt ttgggaatcc tgtctcagga gtttggccat gaacggcaga
      481 ttggtgacct gcggtgcaac cacaggacca ttggtgaata tggatttgcg ccacgtcttc
      541 atcaaacaac aaaagatcat cggatcgacc atggggtcgt tgtcagaaat gttgctcatc
      601 aacaaattca tggcgcagaa aaaactgaaa ccggtgatct cctcagtgtt tccctttacc
      661 gaggtggcac aggcgcagag gtattttgag gaatcgcggc attttgggaa ggtgattttg
      721 agttggcata aggcataagg cgcaaggcgt aagacaagag agaagccatg ggcaattgac
      781 gcaattagca attggggatt gatagttgag tattgataat tgattattgg ttattggtta
      841 ttgtgtgtgg gtaacgggta atgcttcaat atttaaaaaa aattgtgggg gtcaagattg
      901 ccctgtttgt gacggggacg ctgttgtttg tgttttctat tcttttgttt gtggcgaacc
      961 cttttcttga tcggttggca tccggtcagg gagggcaaac ggttttcttg ttgttgaccc
     1021 tgttttttat ctgcctgcag gttgttctga tttttttggc cattggccta ttcatcggac
     1081 gaccattgca gaaaatcatg gcatccatgc gacgcattgc ccaaggtcaa acagaagaaa
     1141 tttgcgacgt gggcgaaggt gatgagtttg gcgagcttag tggcaactta aataagatga
     1201 tgcaaaacat caggcacatg ctcgatagtc gccagaagat tgagaaaagg ctggtggcgg
     1261 ccgaagaagg cctcaaatac aaatccgccc tcgaagacaa agccaagatc attgaacgga
     1321 tgaacctcga gttgtctggg gccttaagcg acatttcgct cctgtatacg gtgtcgcaac
     1381 atctcagttc tgtgttggcg atggatgacc tgttgcagaa ggttcggttg atttttgaaa
     1441 acaaactgtc gtgtgatgga tttgttcttc tgttggttga caaccccgct cggcaattga
     1501 aactggtggc cagcaagaat ctcaaggatc tgagtgagga acaaaagagc gttgatgtgg
     1561 gagatggtct ggcgggcttt gtggtacaga aaaatcgaag tcattatgtg tccgatttgc
     1621 tttcttttga gcgatcccgc ttgtcggtga ttgaaagcga tatgcagggt tccgtgtttt
     1681 gtgtgccgtt gcgtgtgcga gaagaggttg tgggggtgtt gctggtttgg cgccagcaaa
     1741 tcgattcctt tacgcccacg gacagacaat ctttggagtc cattgggagc cagattgccg
     1801 tggccttcga tcgttcgctc ttgcacacga agacaaaaga attatccgtc agggatgagc
     1861 tgaccgatgt gtacaacagg cgttactttt atcaaatgct ccacagagaa tttgagcggg
     1921 ccaaacgctt caatcggccg ctatcattga tcatgattga tctggatcat ttcaaacgtc
     1981 tcaacgatac ctttgggcac attgtgggag acggcgtgct caaaaaattt gcccagttga
     2041 tcagggacca tcttcgcgaa attgatgtcc tggcgcgatt tggtggtgaa gaatttgttg
     2101 ttttactatc cgacacagcg atgaacaatg ctgttatcgt ggccgaaaaa ctacgaagcc
     2161 tggttcgggt gcaatcgtgg gagcctttat tgtccggcgg agacgatagg ggtgatatga
     2221 tgatcacgat cagtgttggt gtggcgggat ttcccgaaac ggcgaagacc caagaagagt
     2281 tggtgcatag ggctgacatc gcattgtatc aggccaaaaa aatgggtcga gacagggtgt
     2341 gtgtgtttca acctgatcag gatgccctga tgagcagtga tctaggtgcg tgcgaggagt
     2401 tgctttcgga caatgttttt cccatagatc gcgccaagct aagcgattga cataaatcat
     2461 tctccatgcg atgctcatgt ggcgacgaat tttgaatgat aacgttttgt acttgtttcg
     2521 aatttcgtaa ttcggattta gaatttcccg ctgtgtcggg tggggggtca tatgaatttt
     2581 aagttgttgt tggatgaagc cattaatcgt ggtgagaaga tcaaacaaga ggttttggca
     2641 gaggtcctaa aatcaaagac catccaagag attgtgtcga acaaaaactt cgttaaggca
     2701 cttaccaaga tcatcgagac aaaacaagaa atcaagcacg tgctcgacaa gcaaaccaag
     2761 tcaatattgg ccctgatgga aattccctcc agaaaagaga tcacagattt gttgcgccaa
     2821 cttgtcaaaa tcgaaggact tatcaataga gtgactcaca aatccacagt tgccaaaacc
     2881 cataaatcag ttcgggctca acgcgtccgg accaccaaac cacgttctgc tgccaaaaaa
     2941 agagccttaa aaaaacgcaa aaaataacac tattaatggg tgttcgcccc cctaagtatt
     3001 tctaaacatt ctttttttga ggtgttttca ccccactttt gggttgggct gaggaacaga
     3061 tgtattaata tataataata tcaaacgatt actggtatgt ttttttttgt cctttggtct
     3121 aaggattgct ttagtaatag gtatgaatag gggcaatcat catgcaaata acccagtagt
     3181 acaaataaaa agtaacccaa cgaatagttc gtgttgttca tcgttatctc gcgagaccgt
     3241 tagcgacttg gagttggctt tgaccaattc tgcgataagc tatgagtatg ctctgaatgc
     3301 cttggataaa atcgattcat atgggattat tgctgacaga ctgatttcgg aattagaaca
     3361 gcacaaacaa aaatatttcg aaattcgatc aaagttattt tccattgatt tacattcttt
     3421 ggttaccatc gagaaaaaat tgattgaaca aaagaaggtc atgttttcaa atcaaacctg
     3481 tctccaataa taaaaaatct gaattatctc gggactatct gcttgacatc ggaactaacc
     3541 tcatttaatt cgcgtcactc gtttgcctga taaaatcacg ccgacctagc tcagctggca
     3601 gagcgactga tttgtaatca gtaggtcatc ggttcgattc cgatggtcgg ctcttagaac
     3661 gactttgcgt tcctgagtat gtcgaaggag cgcttagtcg ttcttagacc ccgctttgcg
     3721 gggttgaaaa tttgttttta cttagccccg cagtgcgggg cgagatatct ctaacgccat
     3781 cgcgtcactt cgtgacacgc tggcataaga gctatctcgg agggatgtcc gagcggctaa
     3841 aggagacggg ctgtaaaccc gttggc
//
LOCUS       DATDUN010000522         3948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_108793, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000522 DATDUN010000000
VERSION     DATDUN010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108793"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..821
                     /locus_tag="VJC18_07350"
     CDS             138..821
                     /locus_tag="VJC18_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45234.1"
                     /translation="MAKNDDIISKMDELMNEHLAQKKVARRNALIYSGVLVVMLFTFA
                     FIIKGNIIDNFSQASIEKSFNERFATIEPLLPAYLEALVDGVPEAYAKALSTELQTKL
                     PLIQANFIKSTTTLTADLKKHLEQKLNKELGNNFVKEQIAIISGSSNNVKKAQEASDL
                     IEKQVGELIVSVTSNFQKMFEHDVNALLDVAKTFPRDDKSLLSEQDATKMLMHYLITL
                     VDTELLAMK"
     gene            855..1571
                     /locus_tag="VJC18_07355"
     CDS             855..1571
                     /locus_tag="VJC18_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45235.1"
                     /translation="MDEMIKSNDINEVASNFERFLKKEKEGLKKRNIVYIVSAALVFC
                     FFTYIAIFTNHYVGPNLAFDYVQVKGIKQIAQINMSVKTAAPRVVSQWSNTLVTTMPN
                     VFRQKMFGLMTPIIDTFADKFATDLRLELANLYKQDGMKAVLAGKVDVEEGYKRLFDQ
                     VNLSYTTQIANFYVNNVKAVNNLTNDLLRLENSVNLTEREKYAKKVIGAWLSLLDHKG
                     AIWDVDKAVAEIKSSKKVKK"
     gene            complement(1645..2172)
                     /locus_tag="VJC18_07360"
     CDS             complement(1645..2172)
                     /locus_tag="VJC18_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLD45236.1"
                     /translation="MKTSPVLQCIFNRRSVRKLKADTLPKEDVELMLACLEAAPNAGN
                     LQPWSFYVVTNQAVKDELCQLSFNQEVVKQAPVVFVVCAKPEISATQYGEAGRNLFCL
                     QDTAAAVQNLLLAAASMDYGAVWIGVIKEKEIADCLHLAQDERPVAIIPVGCANEEPC
                     QAERVGWKKITKWIE"
     gene            complement(2169..2834)
                     /locus_tag="VJC18_07365"
     CDS             complement(2169..2834)
                     /locus_tag="VJC18_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HLD45237.1"
                     /translation="MKIHHIIFDWAGTLYDDQHPSYLTSRQVIKELSGRSLSFEQYRK
                     HFTLPVLPFYRRYGVELSLDKINEYFFARYQENCFKGRLFDGVLEALAYFKKQKISMS
                     LFSTMDQKLLKLLCVRLRIHKYFTVIHGSVCDKTIELKKHFSRIKKIPEHVLFLGDTD
                     HDIEAANQDGAWSGCVLNGYQHKARLIKAKPDFVFDDQTQWVPFMRTLTPPVGLGKPG
                     KIK"
     gene            complement(2997..3416)
                     /locus_tag="VJC18_07370"
     CDS             complement(2997..3416)
                     /locus_tag="VJC18_07370"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HLD45238.1"
                     /translation="MSEHQKHVDLICSYSFCAAHRLWNEHWTKKKNDQFFGSCAHVHG
                     HQYKIEVLLSGNINKDTGMLINGYDVDAIVKKYLQDECDHKFLNDDVLFFKKHPPTAE
                     WIAVWLFHGLRAKFPKGVTLKKVRVYETPELAVEYGE"
     gene            complement(3520..>3948)
                     /gene="folE"
                     /locus_tag="VJC18_07375"
     CDS             complement(3520..>3948)
                     /gene="folE"
                     /locus_tag="VJC18_07375"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HLD45239.1"
                     /translation="EDPSAIIKNALFTESIDEMVVVKDIEFYSLCEHHLLPFFGSAHV
                     AYLPHGKIVGLSKIARLVDVFARRLQVQERMTKQIAETLKACLNPLGVAVVIKAEHMC
                     MQMRGVEKRESVMVTSSMMGAFKKNPATRAEFMDFIKSSL"
BASE COUNT         1088 a          980 c          854 g         1026 t
ORIGIN      
        1 aaaatcaaat ttacccactt ttatgtcgca caccccaaac acaataactt attgacttaa
       61 tgtgaaaata ggttaacata gatgcacttt tgtttctcat taacaattat tttcggtcaa
      121 ccttaaggag ggtcattatg gcgaaaaacg acgacatcat cagtaagatg gacgaactga
      181 tgaacgagca tttagctcaa aagaaagtgg cgcgcagaaa cgcgctcatt tattctgggg
      241 ttcttgtggt tatgttgttc acgtttgcct tcatcatcaa gggcaatatt atcgacaact
      301 tctcacaggc atcaatcgaa aaatcattca acgaaagatt cgcgaccatt gaaccactgc
      361 tcccggccta tctggaagct ttggtagatg gtgttccaga ggcctatgcc aaggctttgt
      421 ctaccgaact tcagacaaag cttcctttga tccaggcgaa ttttatcaaa tcgactacca
      481 cgctgacagc cgatctcaaa aagcatctcg aacaaaaact caacaaagaa ctgggaaata
      541 acttcgtaaa agaacagatc gccatcatct ccggctccag caacaatgtc aaaaaagcgc
      601 aggaagcctc tgacctcatc gaaaaacagg tgggtgaatt gatcgtgagc gtcacttcga
      661 actttcaaaa aatgttcgaa cacgatgtga acgcactcct cgatgtggcc aaaacgtttc
      721 cccgagacga caagtcgctt ttgagtgaac aggatgccac caagatgctc atgcactacc
      781 tcattaccct ggtcgacact gagcttttgg ctatgaaata acgatggaca aacttcatca
      841 aataaggagt ttttatggac gaaatgataa aatcaaatga cataaacgag gttgccagca
      901 acttcgaacg attcctcaaa aaggaaaaag aaggcttgaa aaagcggaac attgtctaca
      961 tcgtatcagc cgccttggtg ttctgttttt ttacttacat tgccatcttc acaaatcact
     1021 acgtcggccc aaatctggcg tttgattatg tccaagtcaa gggcattaaa cagatcgccc
     1081 agatcaatat gagcgtcaag accgcggcgc ccagagtggt ctctcagtgg tctaacacct
     1141 tggtcaccac gatgccaaac gtgttccgtc aaaaaatgtt tgggctcatg acaccaatca
     1201 tcgacacctt tgccgataaa tttgcgaccg acctcaggtt ggaattggcc aacctctaca
     1261 agcaagacgg gatgaaggcc gttttggcgg gtaaggtgga cgtggaagaa ggctacaaga
     1321 ggttgttcga tcaggtcaat ttgtcttaca ccactcagat tgccaacttt tacgttaaca
     1381 acgtaaaggc cgtgaacaat ctgaccaacg acctgctccg tctggaaaac agcgtcaacc
     1441 tgactgagag agaaaaatat gccaagaagg tcattggcgc ttggttatct ctgctcgatc
     1501 acaagggtgc catttgggac gtagacaaag ccgttgccga gatcaaatcc agcaaaaagg
     1561 tcaagaaata acacatccca atatctaata acagttttca atccctcacc aacttatgaa
     1621 catcagttgg tgagggattt tttttcattc aatccatttc gtgatttttt tccaccccac
     1681 tctttcggcc tggcagggtt cttcgttggc gcatcccacg gggatgatgg cgactggtcg
     1741 ttcgtcttgg gccaaatgca gacagtccgc gatctctttt tccttgatca caccaatcca
     1801 cacggcaccg taatccatcg aagcagcggc cagcaataaa ttttgcaccg ccgccgctgt
     1861 gtcctgaaga caaaacaagt ttcggcctgc ctcaccgtat tgcgttgcag atatttccgg
     1921 tttggcacac acaacaaaca ccacgggggc ctgtttgacc acttcctggt tgaaggacaa
     1981 ctggcacaac tcgtccttaa cagcctgatt ggtcaccacg taaaaagacc agggttgcag
     2041 attacccgca ttgggggccg cctccaggca ggccagcatg agttccacgt cctctttggg
     2101 aagagtatcc gctttgagtt tgcggacact gcgtcggtta aaaatacact gcaacactgg
     2161 tgaggttttc attttatttt ccctggtttg ccgaggccca caggcggcgt gagggttcgc
     2221 ataaagggga cccattgcgt ttgatcgtcg aatacgaaat ctggtttggc tttgatgagt
     2281 ctggctttgt gctggtatcc attcaacaca caaccactcc acgccccatc ttgattggcc
     2341 gcctcgatat cgtgatcggt atcacccaga aacagaacat gttctggtat tttttttatg
     2401 cgcgagaaat gctttttgag ctcaatggtt ttgtcgcaca ccgaaccgtg gatcacagta
     2461 aaatatttat ggattctgag cctcacgcac aacagcttca ggagtttctg gtccattgtc
     2521 gaaaacagac tcatggagat tttttgtttc ttaaaatacg ccagggcctc caaaacacca
     2581 tcgaacaacc tgcccttaaa acagttttcc tgataacgtg caaagaaata ttcgttgatt
     2641 ttatcaagcg acaattccac accatagcgc cgataaaacg gcagaaccgg caaggtaaaa
     2701 tgtttgcgat attgttcaaa actcaggcta cgcccactga gctctttgat gacttgcctg
     2761 gacgtcaggt agctggggtg ctgatcatcg tacagcgtcc cggcccagtc aaagatgatg
     2821 tggtgaattt tcataatacg tggatcgaca atcgtggatc gtggatcgct tgtgcccttt
     2881 agggtatgga tcgtcatcaa ctgtagcaat gactgcaatt gttgcaatca ttgcaattgc
     2941 cgcaattgct acgattgccc attgcaaatt gctccattgt ttcgcccctc gttccctcac
     3001 tccccatact ccaccgccag ttccggggtt tcgtagacac gaactttttt tagagtcacc
     3061 cctttgggaa atttggctct gaggccatga aacagccaca cagcgatcca ttcggcagtg
     3121 ggcgggtgtt ttttaaagaa gagcacatca tcattgagaa acttgtgatc acactcgtcc
     3181 tgcagatatt ttttgacaat agcatccaca tcgtaaccat taatgagcat gcccgtgtct
     3241 ttgttgatat taccgctcag caacacttcg attttgtatt ggtggccatg gacgtgcgcg
     3301 caactgccaa aaaactgatc gttttttttc ttcgtccagt gctcgttcca caaccgatga
     3361 gccgcacaga aggagtagga acagatgagg tccacatgtt tttgatgttc agacatcact
     3421 tcgcatctga atatcgaata acgagcaagg aatatcgaat gtcgaagtac agatttcgac
     3481 attccttgtt cgacattcat tattcaatta atccacccat catagactgc ttttaataaa
     3541 atccataaac tcagccctcg tggccgggtt ctttttgaac gcccccatca tcgatgaggt
     3601 aaccatcacg gattcgcgtt tttccacacc acgcatctgc atgcacatgt gttctgcctt
     3661 gatgaccacg gctacaccca gagggttgag acaggccttg agcgtttcgg cgatctgttt
     3721 ggtcatgcgc tcctgcactt gcagtctgcg cgcaaacaca tcgaccaaac gtgcgatctt
     3781 ggaaagtccc acgatcttgc catggggaag atacgccaca tgtgccgaac caaaaaaagg
     3841 caaaagatga tgctcacaca gggaataaaa ctcaatatct ttcacaacaa ccatctcatc
     3901 gatgctctct gtaaacaggg cgttcttgat gatggcagaa ggatcttc
//
LOCUS       DATDUN010000523         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_108830, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000523 DATDUN010000000
VERSION     DATDUN010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108830"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..846
                     /locus_tag="VJC18_07380"
     CDS             238..846
                     /locus_tag="VJC18_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45240.1"
                     /translation="MKMFFLVFVLFIMSFPVMAGPVESASCTQGGEFGNLIVITFLKD
                     IELGKLVIKRQKEDANYVRDLTNFKGGEYRLDIAPQGGVTGYTIEYKDSTSSDYQSHS
                     DSLNCAVATVDTIPVVAPTAEPITEPTTPNAMEIGENELTSSRPPAVALAKEGTHELT
                     QGESGQLRQRPDEMTASDVVVVDEDVVEAEESEQTEQEKYNF"
     gene            856..1206
                     /locus_tag="VJC18_07385"
     CDS             856..1206
                     /locus_tag="VJC18_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45241.1"
                     /translation="MKTQCIMFVALVCLTMGVTGVRAEVTMMSMQEHRAEVNRIICEK
                     KVACQEPIQLDVCVNTWNKVAEIEIAEQGVEKITALRSVMCRDYLRQKATCDNLTEIY
                     KEGECVLSQLTNTQ"
     gene            complement(1302..2075)
                     /locus_tag="VJC18_07390"
     CDS             complement(1302..2075)
                     /locus_tag="VJC18_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45242.1"
                     /translation="MTITSIQNTSYRSSNDFDLGLLEASSPPLQNTDTLAQAGPSLAT
                     LGTTGKTQSALFVEDANAFLTSRSPANTPTETAWVNHSATWRITELERRTRNINNELS
                     QLGFNSRDYDHRLDKLEGLRRTFLTYQPEQQPIEVELIHHRPYPPHPRPYPAPYEHDE
                     HRVDSLEAEMRRNGLDFIRIEGDYARIDDRIIRLEQRRSDEGFRIGPNPVHDGPGFGI
                     ESRLDQLEANIRRLEGRVNDLRRLSGYCDDRLRRLERIG"
     gene            complement(2210..>2577)
                     /locus_tag="VJC18_07395"
     CDS             complement(2210..>2577)
                     /locus_tag="VJC18_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45243.1"
                     /translation="LIVLRADAVAVMAQKFPDETVDELIFFYPNPTPKKRQANQRFFV
                     GAQFEVFDRSLKTHAILLLATNIKAYLDEAECFLKQVWGYHILSCGPLETAHIPRSAF
                     EKKYHARGDVLFELIAQKP"
BASE COUNT          622 a          580 c          712 g          663 t
ORIGIN      
        1 ctcagggagc ccctccttat caccgcaagg cgggatcccg tttgcgggac aaaaaggagg
       61 ggcggtgccc tcctgttgct tgttgcgcaa taagatttca aagagaaaag ccttttttac
      121 ccacttttat gtcgtacacc cctttcatcc tccattgaca cctctccaaa attatcacat
      181 atcggcccgg gcgggcattc gttgggtgtc ttggtgccag cttctttggg agttaatatg
      241 aaaatgtttt ttttggtgtt tgtattattt attatgtctt ttcctgtcat ggcaggtccc
      301 gtggaatcgg ccagttgtac gcagggtggt gaatttggga atctgattgt catcactttt
      361 ctcaaggata tcgaactggg caaactcgtg atcaaacgtc aaaaagaaga cgcgaattac
      421 gtacgtgatc tgaccaattt caagggcggt gagtaccgac tggacattgc gcctcaaggt
      481 ggtgtgacag gatacaccat tgaatacaag gacagcacca gtagcgacta tcaaagtcat
      541 tcggattctc tgaattgcgc tgtggctacc gtcgacacga ttcccgttgt ggcgcccaca
      601 gcagaaccca tcacggaacc aacgacaccg aatgcgatgg agattgggga aaacgagctt
      661 acgagctcac gcccgcccgc cgtagcttta gcgaaggagg gaactcacga gcttacgcag
      721 ggagaatctg ggcaactgag gcaacgcccc gatgaaatga ctgccagcga tgtcgttgtt
      781 gttgatgagg atgttgtgga ggccgaggag tcggagcaga cggaacagga aaaatacaat
      841 ttttgaggag gcggcatgaa aactcaatgt atcatgtttg tggcgttagt gtgtctgacg
      901 atgggtgtta cgggtgtgcg cgcagaggtg accatgatga gcatgcagga gcatcgtgcg
      961 gaggtgaacc ggatcatctg cgaaaaaaag gttgcctgcc aggaacccat tcagctcgat
     1021 gtttgcgtga acacctggaa caaagtggca gaaatcgaaa tcgccgagca gggcgtggaa
     1081 aagatcaccg ctcttcgttc cgtgatgtgc cgtgattacc tgcgtcagaa ggcaacctgc
     1141 gataacctca ccgagattta caaagaaggc gagtgcgtcc tgtctcaact gaccaacacg
     1201 caatgaattg ttgtagggtg atcgccacgc ggcgcatagc atcagaattg ccttagggca
     1261 attctgatgc aacgcatcgc tcgcgatgac aagtcagttt tttagccgat gcgttctaac
     1321 ctgcgcaggc ggtcatcaca ataacccgac agtctcctga gatcattcac ccgcccttcc
     1381 aggcgtcgta tgttggcctc cagttgatcg agcctggatt caatcccaaa gccggggcca
     1441 tcatgaacag ggttggggcc aatgcggaag ccctcgtctg accgacgttg ttcgagtcgg
     1501 atgatacgat cgtcgatgcg ggcataatct ccctcgattc tgataaagtc caacccattt
     1561 ctgcgcattt cagcttcaag agagtcaaca cggtgttcat catgctcata cggtgcgggg
     1621 taggggcgtg ggtggggagg gtatggtcta tgatgaatga gttccacttc gatgggttgt
     1681 tgctcaggct ggtaggtcaa aaatgttctt cgaagccctt cgagtttgtc cagacgatgg
     1741 tcgtaatccc tgctgttgaa tccgagttga gacaactcgt tgttgatgtt gcgcgtccgg
     1801 cgttccagtt ctgtgattcg ccaggtcgca gaatggttga cccaggcggt ttctgttggt
     1861 gtgtttgccg gtgaacgtga tgtcaaaaag gcattggcat cctcgacaaa cagcgctgat
     1921 tgtgtttttc ctgtggtgcc caacgtggca agtgatggtc cggcttgagc gagggtgtct
     1981 gtattctgta ggggcggcga tgaggcctcc aaaagaccca aatcaaaatc attgctggaa
     2041 cgataagatg tattttgaat actggttatg gtcattgtgt ttttctcctt gtttgtacac
     2101 gtagggaccg gcccctacag gcgattaatt gaagaaaaaa tcttagggga agattttgta
     2161 aaagaaaata tctgattcgc ggtttgtttt atgagtcagg ggaattgtat tatggttttt
     2221 gcgcgatgag ttcgaataaa acatcaccac gggcgtggta ttttttttca aacgcagagc
     2281 gtggaatgtg cgctgtttcc aagggaccgc aagagaggat gtggtaaccc cagacctgtt
     2341 ttaaaaaaca ttctgcttca tccaaatacg ccttgatgtt tgtggccaga agaaggattg
     2401 cgtgtgtttt caggctcctg tcaaagacct caaactgagc ccccacaaag aacctctggt
     2461 tagcctgtct ttttttgggg gtggggttgg gatagaaaaa aatcaattca tccactgtct
     2521 cgtcgggaaa tttttgggcc atcacagcca ctgcgtcggc ccgcaagaca ataaggt
//
LOCUS       DATDUN010000524         1380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_483432, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000524 DATDUN010000000
VERSION     DATDUN010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483432"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="clpX"
                     /locus_tag="VJC18_07400"
     CDS             complement(<1..811)
                     /gene="clpX"
                     /locus_tag="VJC18_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HLD45244.1"
                     /translation="MAKNYDDPLSLCCSFCGKSQREVKKLIAGPTVYICDECIDLCND
                     IIAEETIKQEQQRAKTLVPKPKDIKKVLDEYVIGQDRAKKILSVAVHNHYKRIESKAM
                     SKDIELQKSNILLIGPTGTGKTLLAQTLARLLNVPFTIVDATTLTEAGYVGEDVENII
                     LNLLQNADFDKTKAERGIVYIDEIDKISRKSENPSITRDVSGEGVQQALLKIIEGTIA
                     NVPPKGGRKHPQQDFIQVDTTNILFICGGAFFGLDQLISKRLSEKQMGFGSK"
     gene            complement(822..>1380)
                     /locus_tag="VJC18_07405"
     CDS             complement(822..>1380)
                     /locus_tag="VJC18_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLD45245.1"
                     /translation="IIFLGTAIDDVVANLVIAQILFLESEDHEKDIQLYINSPGGVIT
                     SGMAIYDTMQYVKPDVSTICMGQAASMAALLLAAGAAGKRYALPHSRIMIHQPLGGTQ
                     GQATDIEIHAREILRLKDDLNQVLAKHTGQTVKKIAADTERDNFMSGREAKDYGLIDC
                     VVEYRSETEQLQAKEAEKGKDNKKK"
BASE COUNT          281 a          328 c          370 g          401 t
ORIGIN      
        1 gcttggagcc aaagcccatc tgtttttcgg acaggcgttt gctgatgagc tgatcgagac
       61 cgaaaaaggc gccgccgcag ataaacagga tattggtggt atccacctga atgaaatcct
      121 gttgcgggtg cttgcgccca cctttgggag ggacgttggc gatcgtgcct tcgataattt
      181 tgagcagggc ttgttgcaca ccctcgccgg agacgtcgcg cgtgatcgag gggttttcgg
      241 atttgcgcga aattttatcg atctcgtcga tgtagacaat tccgcgctcg gccttggtct
      301 tgtcgaagtc ggcgttttgc aaaaggttga ggatgatgtt ttcgacatct tcgcccacgt
      361 acccggcctc ggtcagtgtg gtggcatcaa caattgtaaa aggcacattc agcaaccggg
      421 ccagtgtctg cgccagcagg gtcttgcctg tgccggtggg accaatcaac aggatgttgc
      481 tcttttgaag ctcgatatcc ttggacatgg ccttggattc aatgcgtttg taatgattgt
      541 gtaccgctac ggacaggatt tttttggcgc gatcctgacc aatgacgtat tcgtccaata
      601 cttttttaat atccttgggt tttggaacga gtgttttggc cctttgctgt tcctgcttga
      661 tggtctcttc ggcaatgatg tcgttgcaca agtcgataca ttcatcacag atgtataccg
      721 ttggacccgc aatgagcttt ttgacctctc gttgggattt tccgcaaaat gagcaacaaa
      781 ggcttaatgg gtcgtcgtaa ttttttgcca cctaaaaccc cttatttctt tttgttatcc
      841 ttgccttttt ccgcttcctt ggcctgcaac tgttcggtct cacttcggta ctcaaccaca
      901 cagtctatta gaccataatc cttggcttct cgtccagaca taaaattgtc tcgctcagtg
      961 tcggccgcga tttttttaac ggtttgaccc gtatgcttgg ccaaaacctg gttaagatca
     1021 tcttttagtc taaggatttc acgagcatgg atttcgatat cggtagcctg cccttgtgtg
     1081 cctcccaagg gttggtgaat catgatgcgt gaatggggca gggcataccg tttgcctgcg
     1141 gctcctgcgg ccaggagcag ggcggccatg gaggcggctt gtcccataca gatggtggag
     1201 acatcgggtt tgacgtactg catggtatcg taaatagcca tgcccgaggt gatcacccca
     1261 ccgggggaat tgatgtagag ttggatgtct ttttcgtgat cttccgattc cagaaagagg
     1321 atctgcgcga tgaccaagtt tgccacgaca tcgtcgatgg ctgtgccgag gaagatgatg
//
LOCUS       DATDUN010000525         1669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_276693, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000525 DATDUN010000000
VERSION     DATDUN010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276693"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VJC18_07410"
     CDS             <1..558
                     /locus_tag="VJC18_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HLD45246.1"
                     /translation="MVIMARKLHSQPASQPASQIGIPIGNLTSQFFANIYLNRLDYFV
                     KEKLGCKHYIRYQDDFVLFSNDKSLLSIWLQKIRIFLDTNLALSLHENKCKIFSVETE
                     VPFLGFVFCGKRVRIKRASIVRFKRRMKYFSKGLLQRTVRWKTVDQSVMSWIGHVKHA
                     DTYNLRKLVLGDIVLQGQGEEGAIV"
     gene            563..784
                     /locus_tag="VJC18_07415"
     CDS             563..784
                     /locus_tag="VJC18_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45247.1"
                     /translation="MRGGSWNNNNPDNLRVAYRNNNRPANANDNIGFRVGFFPSTLKN
                     QSSLHFCERKRVSESPAPSSWSSDRRWHD"
     gene            complement(864..1400)
                     /locus_tag="VJC18_07420"
     CDS             complement(864..1400)
                     /locus_tag="VJC18_07420"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLD45248.1"
                     /translation="MKIYTKTGDDGTTALANGKRVPKDHARVCAYGDVDELNALLGLA
                     VAYVLNKKFKVDLIKIQKDLMSLTSILANPDHHPKKSKVSLSGQDIRFLEKKIDAMEN
                     DLSPLTHFILPGGSVLAGLLHVARTVCRRAERLCVTLSHKEPIPPVCVHYLNRLSDYL
                     FVAARWANKINLEEDVVW"
BASE COUNT          446 a          382 c          423 g          418 t
ORIGIN      
        1 atggtgataa tggcaagaaa attgcacagc cagccagcca gccagccagc cagccagatt
       61 ggaatcccta ttggcaattt gacgtcgcag ttttttgcca atatttatct caatcgcctt
      121 gattattttg taaaagaaaa attgggttgt aaacattaca ttcgatacca ggacgacttt
      181 gtcttgtttt ccaacgacaa aagcttgcta tcgatttggt tgcagaaaat tcgaattttt
      241 ttggatacaa atcttgcgct gagtttgcat gaaaacaagt gcaaaatctt ttccgttgag
      301 accgaagtgc cgtttcttgg atttgtgttt tgtgggaaac gtgttcgtat caagcgggca
      361 agcattgtac ggttcaagcg ccgaatgaaa tatttttcaa aagggttgtt acaaagaact
      421 gttcgctgga aaaccgttga tcagtctgtg atgtcgtgga tagggcatgt gaaacacgcc
      481 gacacttaca acttaagaaa gcttgtactg ggagacattg ttctccaagg tcaaggggag
      541 gaaggggcaa tagtgtaggg ttctgcgcgg cgggtcttgg aacaataaca atcccgacaa
      601 cctccgcgtt gcgtatcgta acaacaacag gcctgcgaac gccaacgaca atatcggctt
      661 tcgtgtgggc ttcttcccca gcactctcaa gaaccagagt tcccttcact tttgtgaacg
      721 gaagcgtgtg agtgagagtc cagccccttc atcctggtct agcgaccgaa gatggcatga
      781 ttgatgccca ccagttttga gtagaaaacg aaagagaccg gtgggcataa catgccgcaa
      841 acaagagcga gaaggtcccc ccatcaccac accacatcct cctcaagatt gattttattg
      901 gcccagcggg cggcgacaaa caaataatcg gacaagcgat tgaggtagtg aacgcacaca
      961 ggagggatgg gttccttgtg tgatagggtc acgcaaagcc tttcggcgcg gcgacacacg
     1021 gtgcgcgcga catgtaacag gcctgccaag accgatcccc cgggcagaat aaaatgcgtg
     1081 aggggggata gatcgttttc catcgcatca atcttttttt cgaggaaccg gatatcttgt
     1141 ccagaaaggg acaccttgct ttttttgggg tgatgatcgg ggttggcaag gatggaggtc
     1201 agtgacatca ggtctttttg aattttgatc aaatcgacct tgaatttttt gttcagcacg
     1261 taggccacgg ccagacccaa cagagcattc agctcatcca catcaccata agcacaaaca
     1321 cgtgcgtgat ctttgggaac gcgcttgccg ttggccagtg cggtggtgcc atcgtcacct
     1381 gtttttgtat agattttcat aagaaatcct aatagtgttc tctttgcgta ggggccggtc
     1441 gagaccggcc ccacacatta cggtactgca aagcaaaatt caaatgataa gaagtggggg
     1501 aacaaatgat catcgaagtc ttacaagtgg ggccgtttca gagtaattgc gtcattttgg
     1561 cagatcacaa aacccgacag gcgaccgtga ttgatccggg agatgacgcc ccagacatcc
     1621 tggccttttt gaaggatgag aaactcaccg tctcccattt gctcctcac
//
LOCUS       DATDUN010000526         2203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_67045, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000526 DATDUN010000000
VERSION     DATDUN010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67045"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VJC18_07425"
     CDS             complement(<1..222)
                     /locus_tag="VJC18_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HLD45249.1"
                     /translation="MTTTLLIIIFLVFVALAFDFINGFHDAANSVATIVATRVLTPKQ
                     AVMWAAFFNFIAAFVFGLHVANTIGKGIIV"
     gene            complement(253..969)
                     /locus_tag="VJC18_07430"
     CDS             complement(253..969)
                     /locus_tag="VJC18_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HLD45250.1"
                     /translation="MQQCDQKNMTHKGCGCDNTKSAPSKLSQMISGLRLFPKTHDFFG
                     LMEQSARNVVKGTELLCQMIENKARREELLVQLKDCEHDGDKITHDTIDLVHSTFLTP
                     FDRTDMRKLLVKMDDVLDRCHSTGNRLTKYNVTEMPDELFKLAQTVRRAAGEILGAVS
                     GLRNIKNVQTVLNHCREINRLEREADELINAAFTTLFDNSWDPLEVIKLKELAEIIEA
                     AADKSKNVSDIIEGIILKNT"
     gene            complement(1114..>2203)
                     /locus_tag="VJC18_07435"
     CDS             complement(1114..>2203)
                     /locus_tag="VJC18_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLD45251.1"
                     /translation="IKGTGPRYCPSIEDKIVRFKDKDRHQIFLEPEGLDTNEYYPNGI
                     PTSLPLETQIKLVRSIKGLERAEIQRPGYAVEYDAINPLQLKANYETKNIRGLFLAGQ
                     VNGTSGYEEAAAQGLLAGINATQLVKGEGEVIIGRDQGYLGVMTDDLVTKGVGGEPYR
                     MFTSRAEYRLILREDNADARLTPLGRQLGLVADEAFARFEQKMRKLSRMRSYIKSSWV
                     KREGHLFFKGVLGDSQMSYPECSSLEQMIKWPEVTIDGFKKSWSHLGSGLDFDDDVMT
                     CLFSETRYQGYIERYEREIARMKVYDQLRIPKNISYDAVPCLSNEARHKFNTHRPETL
                     GQAIRIPGVTPSAVSHLEMYLARRTSKS"
BASE COUNT          547 a          562 c          498 g          596 t
ORIGIN      
        1 aacgataatg cccttgccaa tagtgttggc gacgtgcaaa ccaaaaacaa aggccgcaat
       61 aaaattaaaa aaagccgccc acatcacggc ctgcttgggt gtgaggactc gggttgccac
      121 aattgtggcc acggaatttg cggcatcatg gaacccgttg atgaaatcaa acgccaaggc
      181 cacaaacacc agaaaaatga taatgagcag tgtggttgtc atagttttac atcctgtgaa
      241 caagtggcac aatcaggtgt ttttgagaat aatcccctcg atgatgtcgg acacattttt
      301 ggacttatct gcggcggcct cgatgatttc ggcaagttcc ttgagcttga tgacctctag
      361 cggatcccat gaattatcaa acaacgtggt gaacgctgca ttgattagct catccgcctc
      421 tctctccaga cgattgattt cgcgacagtg atttaaaacg gtctggacat tcttgatatt
      481 tctgagacca gacacggccc ccaagatttc cccggcggct cgacgcacgg tttgcgccag
      541 tttgaacaac tcatccggca tttcagtcac gttgtacttg gtgagccgat tgcccgttga
      601 atggcaacga tccagcacgt catccatttt gaccaacaat ttgcgcatgt ctgttcgatc
      661 gaagggggtc aaaaacgtgg agtgcaccaa atcgatggtg tcgtgagtga tcttgtcgcc
      721 atcatgttcg caatccttga gttgcaccaa aagttcctcg cgacgcgctt tgttttcgat
      781 catctgacaa agaagctctg tgcccttaac cacattccgc gcactttgtt ccatgagccc
      841 aaaaaaatcg tgcgttttgg gaaaaagtct gagtccagaa atcatctggc tcaatttgga
      901 gggcgctgat tttgtattat cacaaccaca ccccttgtga gtcatgtttt tttgatcgca
      961 ctgttgcata ttgtctcctt tagagaaatg ttgaacgcat cctaattggc tcttttgtta
     1021 tcatgagtga aggactgtta caatgcgatt ttttaaaaaa aacacaagcc agaggactca
     1081 aatacaaacc tatctcgctg atattatatg tttttatgat ttgcttgttc tgcgcgccaa
     1141 atacatctcc agatgactca cagcagaagg cgtcactccg ggaatgcgga tggcttgacc
     1201 caaagtctcg ggtcggtggg tattgaattt atgtcgcgcc tcattggata aacagggtac
     1261 ggcgtcataa gaaatgtttt tggggatgcg gagttgatca tacaccttca tgcgggcaat
     1321 ctccctctcg tagcgttcaa tgtaaccctg ataccgcgtt tcgctgaaca aacaggtcat
     1381 gacgtcatca tcaaaatcca gccctgaccc caaatgagac cagctctttt tgaaaccatc
     1441 aattgtgacc tccggccact tgatcatttg ttccagactg gaacactcag gataggacat
     1501 ttgtgaatcc ccgagcactc ccttgaaaaa aagatgcccc tcccttttga cccatgagct
     1561 tttgatgtaa gagcgcattc gcgaaagctt ccgcatcttt tgttcgaaac gggcaaaggc
     1621 ctcgtcggcc acaagaccca gctgacgtcc aagtggcgtg agtctagcgt cggcattgtc
     1681 ctctctcagg atcaaacgat attcggccct cgatgtaaac atgcggtacg gttctccgcc
     1741 aacacccttg gtcacaagat catctgtcat cacccccaaa taaccctgat cacggccaat
     1801 gatgacctct ccctcaccct tgacgagttg tgtcgcattg atcccggcga gcaggccttg
     1861 cgcggccgct tcctcataac cagaggtgcc attcacctgt cccgccaaaa acaagccccg
     1921 tatgtttttg gtttcataat tggccttgag ttgcagggga ttgatggcat cgtattcaac
     1981 cgcatagccc ggtctttgaa tttctgctct ttccaatccc ttgatggatc gcaccagttt
     2041 gatttgggtt tccagcggca aactggtggg aatcccgttg ggataatatt cattggtgtc
     2101 gagcccttca ggttccagaa aaatctggtg gcggtcctta tctttgaatc gtacaatttt
     2161 gtcttcgata ctcggacaat aacgtggccc cgtcccttta atg
//
LOCUS       DATDUN010000527         2470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_397988, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000527 DATDUN010000000
VERSION     DATDUN010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397988"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VJC18_07440"
     CDS             <1..317
                     /locus_tag="VJC18_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45252.1"
                     /translation="ALTEISPRDAEETKTALALTQTLSTFTNDLLRRLEALSQWDLSQ
                     PDQELLSCLVSTQQLYLISPSKILQRLLSQQTTTNTSALAQQWGQLESLWTHKITSPL
                     LS"
     gene            complement(384..782)
                     /locus_tag="VJC18_07445"
     CDS             complement(384..782)
                     /locus_tag="VJC18_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45253.1"
                     /translation="MGNKYLCPKCRGRLDVHEYVAFTAQAKDGDRGLLLLSPHLGNYD
                     VWHNDNFRYQQGDLVKYFCPICHKNLTAIEFHEHLVRIHMVDGDNIEHNVLFSGVAGE
                     EATYKVRDDEIEPYGQHSNQYMQYFISARM"
     gene            1372..2157
                     /locus_tag="VJC18_07450"
     CDS             1372..2157
                     /locus_tag="VJC18_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD45254.1"
                     /translation="MTASEINPSSGHDIVFSPVYGPVKSRRYGTTFGINLLPSGEKTC
                     SFDCLYCQLGWSTKKLPSANTEFPVKELIAKELARLLTKHVNTAPHQQVSIVFSGNGE
                     PTLHPDFANIVSQVIRTRDEISNTTPIVCFTNGTTIQKQVVFDALSLLEECCVKLDPQ
                     LAITNSPPKAFSWPDVKNIIVHLNNLVIQSCVFGGLGGNDSRDSIEQWVEHFSGLNPR
                     RVDLYTISRQTPHAGLIPTSKDRLTEIKNELSRIFSGPITITI"
BASE COUNT          662 a          664 c          495 g          649 t
ORIGIN      
        1 tggccctcac agaaatttcg ccacgcgacg ccgaagaaac caaaacagct ttggcgctca
       61 cccaaacatt gtcaacattc accaatgacc tgcttcgacg tttggaagcc ctgtcacagt
      121 gggatctttc tcaaccagac caagagcttc tgtcttgtct ggtgagcaca caacaactct
      181 atctcatcag cccttcgaaa atcctgcaaa ggctattgag tcaacaaacc accacaaaca
      241 ccagcgcttt agcccaacaa tgggggcaac tggagtccct ctggacacac aaaatcacct
      301 cacctctgtt aagctgagtg atgccacagg ttcttgtggt gcctcacatg aaaactcgtg
      361 gtgtctgtgg agtcgggcaa aactcacatc ctggcgctga tgaaatattg catgtactgg
      421 ttggagtgct gaccataagg ttctatctca tcatcgcgaa ccttgtaagt cgcctcttca
      481 ccggccacgc ccgaaaacaa cacgttgtgc tcaatgttgt ctccgtctac catgtgaatg
      541 cgaaccagat gttcatgaaa ttcgatcgct gtcagatttt tatgacagat gggacagaaa
      601 tattttacca gatcgccttg ttggtatcga aaattgtcat tgtgccagac atcgtaattc
      661 cccaagtgag gactcagtag caaaagaccg cgatcgccgt cctttgcctg cgcagtgaaa
      721 gcaacatact catggacgtc taaacgccca cggcattttg gacaaagata tttattaccc
      781 atacatcctc cttgacatgg cggaccaagc ccacatttct cattggcttt ctacgcaaag
      841 cacggaggaa gtcttgacaa aagcggactg tgttccacca ccgtcttgac tgaaaaaata
      901 atatacgccc ctatcggttt gtgtccaata aggaccctac acaattttat tccagatgtc
      961 tgctggagag catggactca gaaaaaagac aatgggtttt tctcaaagag agacagctgc
     1021 gttatttttc tcttgttttg ttaactaata accattattt acgcaaaaaa tgtttgcgct
     1081 atggctttag ttataataga ctcgtgcgaa caattaactc actaacttcc taagaggaac
     1141 actatgaaga aactcaacac cacaactatc ttactgatcg tattgctcat cgctgccgtt
     1201 atctaccttt tcgtagggta atattcctac catcaagcac tcccccctcc gtatgatctg
     1261 cataaaacca tagctttgat gcgttggttt ttttgtttgg ggcctgctcc ctacttgtta
     1321 aggggcgtag attcctcaac tgcgaatttc taggagggta tttctcatag catgacagct
     1381 tctgaaatca atccatcatc ggggcatgat attgtttttt ctcctgtgta tggccctgtc
     1441 aagtcgcgtc gttatggaac gacctttggc attaatctcc tgccgtcagg cgaaaaaacc
     1501 tgttcgtttg actgtcttta ctgtcagctg gggtggtcta caaagaaatt gcccagcgca
     1561 aacaccgaat ttcccgttaa ggaactcatt gcaaaagagc tcgcccgttt actgacgaag
     1621 cacgtcaaca ccgcgccaca tcagcaggtc agtatcgttt tcagcggaaa cggagaaccg
     1681 acgttgcatc cagatttcgc caacattgtc tcccaggtca ttcgaacgcg tgatgagatt
     1741 tctaacacga cgcccatagt ttgttttacc aatgggacaa ccattcagaa gcaagttgtt
     1801 ttcgacgcct tatctcttct cgaagaatgt tgtgtaaagt tggatcccca actagcgatc
     1861 accaattcac cacccaaggc tttttcttgg ccggatgtaa aaaatattat cgtacacctc
     1921 aacaatctgg tcattcaatc ttgcgttttt ggagggctgg ggggaaacga ctctcgcgac
     1981 tccatagaac aatgggtaga acatttttcg ggactgaatc caaggcgcgt tgatctgtac
     2041 accatctccc gtcaaacgcc tcatgctggc ctgatcccaa catccaaaga cagattgacc
     2101 gaaatcaaaa acgaattgtc ccgcattttc agcggcccga tcaccatcac catttgaatg
     2161 ataccctgaa ccctgctccc tgtgcctttt gccttcttcg tgaacacgtg aacacgccca
     2221 gtaccgtaat ggtaccaagc acacacggat aacttcaccc atcactcgtt actcgttacc
     2281 cgttgaccgt tgtcccttga gcaattatgc ttctcgacat gccaaccacc gacaaaaacc
     2341 aaaatcgtta ttggggatgg gggtatcaaa atcacgaata cgatccccac aaggcctctc
     2401 tatttctgca cctgctcgca caacgtttcg cgctcaacac actggctccc aaacagatcc
     2461 cgccaaccgt
//
LOCUS       DATDUN010000528         1423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_193696, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000528 DATDUN010000000
VERSION     DATDUN010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193696"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VJC18_07455"
     CDS             complement(<1..679)
                     /locus_tag="VJC18_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HLD45255.1"
                     /translation="MNRTERFYLIDKMLAERKFVSRQELIEELSISWATLKRDMAYLK
                     DRFNAPIIHDRELSGYRFDTPNIGPKYELPGLWFNADETYALLAMHQMLSELEPGLLT
                     PHVAPLLSRLEIIVSIDGRPFDEVSKNIRLARTGARQKSPEHFGVITRAILKGRQIHI
                     LHFNRPRNEYTERDLSPLRLVFYRNNWYLECWCHARQALRRFSLDAIDSVRMLDSAAL
                     TVSNQEIE"
     gene            846..>1423
                     /locus_tag="VJC18_07460"
     CDS             846..>1423
                     /locus_tag="VJC18_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45256.1"
                     /translation="MKFQNLFVVVMATVVLSGCGTLPPLNFSVPNVGVATHKVDAEMK
                     SLTVTVARPDEKIGNLDLQFASIDVNGQGGGAGDQMVPQMWQTALTEALNRMVVFQDE
                     AAKKVNLSVKILKLDVPRAGVAFTTNTTARYEIMDRKNGDLIFSQDIASSGTTPMDFS
                     MLGLARMRESINRAVQNNITQFLQSLETVDVQ"
BASE COUNT          388 a          315 c          347 g          373 t
ORIGIN      
        1 cttcaatttc ctggttcgac accgttagcg cagcactatc cagcatccgg acgctatcga
       61 ttgcatccaa agaaaatcga cgcaatgcct gtcgtgcgtg gcaccagcat tccaaatacc
      121 aattgttgcg ataaaaaacc aatcgcaacg gcgacaaatc acgctcggtg tattcgttgc
      181 gcgggcgatt gaaatgcagg atgtgtattt gtcttccctt aagaattgct cgggtgatca
      241 cgccaaagtg ttcagggctt ttttgtctgg ctcccgttcg tgcaagacgt atgtttttgg
      301 acacctcatc aaatgggcgt ccatcaatgc taacaatgat ctctaatcgc gatagcaacg
      361 gcgcgacatg aggggttagc aatccgggtt cgagttcgga caacatttgg tgcatggcaa
      421 gcaaagcata cgtttcatcc gcattaaacc aaagtccggg caattcatac ttggggccaa
      481 tattgggcgt atcgaagcga tagccgctta attcccggtc atgaatgatg ggtgcattga
      541 atctgtcttt gagataagcc atgtctctct tgagtgtcgc ccaagaaatg gacagttcct
      601 caatcaattc ctgacgagat acgaacttac gctcagccaa cattttgtcg atcaaataaa
      661 aacgttccgt acgattcata acatagctcc aggggcgcac tttctgagcc ccgagtatga
      721 caccatccaa cagaactcac cgaataaatt attttcggtg agagtggtgt aaagaatggg
      781 ggcctctttc catgccggtg gagagttgct attttaatcc cggtgaaaac cctaagaggc
      841 tcgatatgaa atttcagaat ttgttcgttg tggtaatggc aacagttgtt ttatcgggct
      901 gcggtacatt gcccccatta aatttttccg ttccgaatgt tggggttgcg actcataaag
      961 tggacgccga gatgaaatcg ttgacagtca ctgttgcccg tcccgatgaa aaaattggta
     1021 atttggattt gcaatttgcc tccatagatg tgaacggaca gggcggtgga gcaggagacc
     1081 aaatggtgcc gcaaatgtgg cagacggcac tgacagaagc tctgaacagg atggtggtgt
     1141 ttcaggatga ggcggcaaaa aaggtcaacc tttcagtaaa gatattgaaa ctcgatgtgc
     1201 cacgagctgg tgttgcattt accaccaata caactgctcg ctatgaaatc atggacagaa
     1261 aaaatggaga tctgattttt tcacaggata ttgcatcttc tggcacaacg ccgatggatt
     1321 tttcaatgtt aggattggcc agaatgcgtg agtctatcaa tcgcgcagta caaaataaca
     1381 ttacccagtt cttgcagtcg ctggaaacag ttgatgtgca aaa
//
LOCUS       DATDUN010000529         1930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_150065, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000529 DATDUN010000000
VERSION     DATDUN010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150065"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VJC18_07465"
     CDS             <1..1276
                     /locus_tag="VJC18_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HLD45257.1"
                     /translation="FLVPGRGKNRETPICLQDPGRGFVFKKHDCENEKMLSYQTEVTP
                     AYEKADEVWRHKEALELSRLVYVAMTRASRCLYFFVEKPSQSLLSRFAGDAENTQLIK
                     KYNEWLYWLAKVICGDEIKNEYHPVAALPKQTGSVSETNLEKEFVYSYSHLSSLQSPI
                     TYTVTELETYQHCPRRFALRHGQNLAPVRHRTSPDRSPRPPQTRHQLRPSERGNLFHE
                     ILQYYDFKTGRNLDEVIDQALFNQHILASSDNIRTECHLFINGLKKDVVLSEILFGPG
                     DSDVEVAFSYRLDQFVLTGQIDRVVCLRGAQHKNRYVIVDYKTHHAVSDEQRSALMQQ
                     FTFQMSCYALAWSQAIKAETLDTLVLFTSVPTYRIITHTAQTLSAFEGQLNSLFSAIQ
                     DSIRQARFPLTLDQTHCMNCPYFAKNYCGVKT"
     gene            1289..>1930
                     /locus_tag="VJC18_07470"
     CDS             1289..>1930
                     /locus_tag="VJC18_07470"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HLD45258.1"
                     /translation="MFKHVFQSPFFWVFLFFYVANEITHFVLEMINLRFVARQTSVPE
                     AFHGHITQETFEKAQAYTIEKTKFALVTRLIGIPFFWMLIFMGGLQVMDGFAAEWVGA
                     GTLTQSVLFCLLVGAYFFFIGIPFKIYSTFVIEENHGFNQITPALFARDLVKSILLSL
                     VLGTPVLYAVFWFMDHAGENWWLWVWLVLALFQLFVITVYPTWLAPLFNKFKEV"
BASE COUNT          449 a          470 c          490 g          521 t
ORIGIN      
        1 ctttctggtc ccggggcggg gaaaaaaccg cgaaacgccc atttgtctcc aggaccccgg
       61 cagggggttc gtgtttaaaa aacatgattg cgaaaacgaa aagatgctct cgtaccagac
      121 agaagtcact cctgcctatg agaaggcgga tgaggtatgg cgacacaaag aggcgttgga
      181 attatcgcgt ttggtgtatg tggccatgac tcgggccagc cggtgtctct acttttttgt
      241 ggaaaaaccg tcgcagtcct tgttgagccg ctttgcggga gacgctgaaa acacgcagtt
      301 gattaaaaaa tacaatgagt ggttgtactg gctggccaag gtgatttgtg gcgatgaaat
      361 aaaaaatgaa taccaccccg tggcggcact gccaaagcag actgggtctg tttccgaaac
      421 aaatctggaa aaggagtttg tttattctta ttcccatttg tcttcgttac aaagtccaat
      481 cacctacacg gtcaccgagc tggaaaccta ccagcattgt cccaggcgtt ttgccttgcg
      541 tcatgggcaa aacctggcac ctgtgcgtca caggacctct cctgatcgat ccccacggcc
      601 accccaaaca cgccatcagt tacgccccag cgagcggggc aacctgtttc acgaaatcct
      661 gcagtattat gatttcaaaa cgggtcgcaa tctcgatgag gtgattgatc aggccttgtt
      721 caaccaacac atcctggcgt catccgacaa catcaggacc gaatgtcatt tgttcatcaa
      781 tgggcttaaa aaagatgtcg tgctcagtga gattcttttc ggtcccggag attccgatgt
      841 tgaagttgcg ttttcgtatc gcctcgacca gtttgtattg acgggacaga tcgacagggt
      901 ggtttgcttg aggggggcac aacacaaaaa tcgttacgtg atcgttgatt acaaaacaca
      961 tcacgcggtg agcgatgaac aacgatcagc cctcatgcag caattcacct ttcagatgtc
     1021 gtgttacgcc ctggcctggt cccaggccat taaggcagaa acgctggaca ccttggtgct
     1081 gttcacaagt gtgcccacgt accggatcat cacgcatacg gcacagaccc tgtctgcctt
     1141 cgagggacaa ctgaacagcc ttttttcggc gatacaggac agcatcaggc aggcgcgctt
     1201 tcccctcacc ttggatcaaa cacattgcat gaactgtccg tattttgcca aaaattattg
     1261 cggtgtgaag acctgaagga attattttat gttcaaacat gtttttcagt cccccttttt
     1321 ctgggttttt ctgttttttt atgtggcgaa cgagatcacg cactttgtgc tggaaatgat
     1381 caatctgcgg tttgtggcca ggcaaaccag tgtgcccgaa gcctttcatg gacacatcac
     1441 acaggagacc tttgaaaagg cccaggccta caccatcgaa aaaacaaaat ttgcgttagt
     1501 gacccgtcta atcggcatcc cctttttctg gatgctgatt ttcatgggag ggttacaggt
     1561 gatggacggt ttcgccgcgg agtgggttgg ggcgggcact ctcacccagt cggtgttgtt
     1621 ctgtttgctg gtgggggcct atttcttttt catcggcatc ccgttcaaga tctattccac
     1681 ctttgtgatt gaggagaacc acggcttcaa tcaaatcaca ccggcactct tcgcgcgtga
     1741 tctggtcaaa tcaatcttgt tgagtctggt gttggggact cccgtccttt acgccgtgtt
     1801 ctggtttatg gatcacgctg gagagaattg gtggctttgg gtttggctgg ttttggcctt
     1861 gtttcaattg ttcgtcatta cggtttatcc cacgtggctg gctcctttgt ttaacaagtt
     1921 taaagaggtt
//
LOCUS       DATDUN010000530         1037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_562012, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000530 DATDUN010000000
VERSION     DATDUN010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562012"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VJC18_07475"
     CDS             <1..610
                     /locus_tag="VJC18_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HLD45259.1"
                     /translation="TYPEVTEMQVRAILEATVELLQEGKKVLPEIMIPVVVVESELIH
                     QKNIALKIHDEVCQKYGVKKINHLFGTMIETPRAALTAGQVAQTAEFFSFGTNDLTQM
                     SFGFSRDDVGGFLPDYLDKKILPADPFQTLDQAGVGELIQIAVTRGRKTRDHLKVGIC
                     GEHGGDPASVAFCHRVGFDYVSCSPYRVPIARLSAAQEALRG"
     gene            720..>1037
                     /locus_tag="VJC18_07480"
     CDS             720..>1037
                     /locus_tag="VJC18_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45260.1"
                     /translation="MIDKNTKGIVVIVLILLFTACASETGIFPSVDLSTSEANAVLPN
                     PISIVSDAANAQIVVANSNVDILYDTGSLAVLGFDATDTDAPVLTAQKIIATPNFATD
                     MYFD"
BASE COUNT          250 a          250 c          293 g          244 t
ORIGIN      
        1 aacgtacccg gaagttaccg aaatgcaggt gagggccatt ttggaagcga ctgtggaact
       61 gctgcaggaa ggcaaaaaag tgttgcccga aatcatgatc ccggtggtgg tggtggagag
      121 tgaactcatt caccagaaaa atattgccct gaaaattcat gatgaggtgt gccagaaata
      181 cggtgtcaaa aaaatcaatc acctgtttgg caccatgatc gaaacgccac gagcggcact
      241 gacagcgggt caggtggcgc aaacggcgga gttcttctcg tttgggacca acgatctcac
      301 gcagatgagt tttggttttt cgcgtgacga tgtgggaggc ttcttgccgg attatctcga
      361 taaaaaaatc cttcccgccg acccgtttca gactcttgac caggcaggag tgggcgaact
      421 cattcaaatc gctgtgacac gggggcgcaa aacacgtgat cacctcaagg tggggatttg
      481 cggagaacat ggtggtgacc ccgcctcggt cgcattttgt catcgtgtgg gatttgacta
      541 tgtgagctgt tcaccgtatc gggtgcccat tgccagattg agcgcggcac aggaggcgtt
      601 gaggggatga ggggggcaac cttacgagct caagagctta tcagcgcacg agctcacgaa
      661 aaaaacagcg tacgctcgtg agctcgtgag ctcgtgagtt cgtaataggg ggtatcgcta
      721 tgatcgataa aaatacaaag ggcatcgttg tcattgtcct catcttgcta ttcacggcct
      781 gtgcctccga gacgggtatc tttccgagtg tcgatttgtc gacctcagag gccaatgctg
      841 tcttgcccaa tcccatcagt attgtcagcg atgcggccaa cgcacagatc gtggtggcca
      901 attctaatgt cgatatcctg tacgacacgg gttccttggc ggtacttggt tttgacgcga
      961 ccgacaccga tgctccggtg ctgactgccc agaaaatcat tgccacgccc aactttgcaa
     1021 cagacatgta tttcgat
//
LOCUS       DATDUN010000531         1983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_277166, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000531 DATDUN010000000
VERSION     DATDUN010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277166"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..471)
                     /locus_tag="VJC18_07485"
     CDS             complement(187..471)
                     /locus_tag="VJC18_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45261.1"
                     /translation="MTKRHKVATVAIVFCVFLLSSCALLDRSIIAISEEMGFNTKRDP
                     LGYTLDDKTSYRKHKFFLKTQKREREKELLRRLQESNGNPQTKSDVHSPR"
     gene            495..1385
                     /locus_tag="VJC18_07490"
     CDS             495..1385
                     /locus_tag="VJC18_07490"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HLD45262.1"
                     /translation="MKTSRNLVPHFVIFVLFLLFCPEVRAGSDHSLSLPHDGHLRIFA
                     YHVGEYLDVTYLDQNGDWDHTAYVRLNFLLRSRGDDQTTQMDKRLIELADHLQDHFKT
                     DGIEIISGYRSPEFNQELREQGHRVAEESYHTKGMALDVHLDEIREDVLRDYLLSLKL
                     GGVGYYGDRLMVHMDFGPLREWHSGDFSENTKIGVFNDKNRVRITTDKFYYALADPQN
                     LVFEGIEPVAGHQMQLQKFLRGQWQDVTGVVLFGHMSSIANLQTLMSSVVATSPYGKF
                     RWQWRHGEAWQNSNEFYIKK"
     gene            1382..1951
                     /locus_tag="VJC18_07495"
     CDS             1382..1951
                     /locus_tag="VJC18_07495"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HLD45263.1"
                     /translation="MILLIDNYDSFTYNLYQYLMELGALVQVHRNDQITLDDIATLRP
                     AKIVISPGPCTPNEAGISMDVVKRFAGQIPILGVCLGHQALAQAYGGSIIRAPKIMHG
                     KTSPIFHNGRGLYRGLPNPFLATRYHSLIVERESLPSLLEVTAWTEDQLIMGMGHRDY
                     PVYGVQFHPESILTTQGHALLRNFLDQVT"
BASE COUNT          497 a          426 c          503 g          557 t
ORIGIN      
        1 ttgaggacca gatagccgat aaagaccatc gtgccgacaa tgggtatgat gttgcacaac
       61 agaccaatga gtaaaatcat ccaccagctg gtgttctcaa aaatatattt gactgagcgc
      121 gtgtaattca tttctgtttc accgatgtgc ctttagcttg aatgagacat cgtggatcat
      181 gacacctcat cgtggcgagt gcacgtcgga ttttgtttgg gggttcccat tgctttcctg
      241 caatcttctt aaaagttcct tttctctttc cctcttctgc gtttttaaaa aaaatttatg
      301 tttacgataa gacgttttat catccagcgt gtagcccaaa gggtcacgtt tggtgttaaa
      361 gcccatttcc tccgaaatgg caatgatcga tctatcaagc agggcgcaag agctcaatag
      421 aaaaacacag aaaacaatgg cgactgttgc aactttgtgg cgtttcgtca tagtgttgac
      481 gcaggagtca atgaatgaaa acaagccgca acctcgtacc acattttgta atatttgtcc
      541 tctttttgct attttgtcct gaagtccgtg ctggttcaga ccattcactc tccttgcccc
      601 acgatggaca cttaaggatc tttgcttatc acgtggggga atatcttgat gtgacttatc
      661 tggatcaaaa tggagactgg gaccacacgg cctatgtgag actgaatttc ctgttgcggt
      721 ctcggggtga tgaccaaacg acccagatgg acaaaaggct cattgagctg gcggatcatt
      781 tgcaggatca cttcaagaca gatggcattg agatcatcag tggttaccgg agcccagaat
      841 ttaatcaaga actaagagaa cagggacacc gtgtggctga ggagagttat cataccaagg
      901 gaatggcctt ggatgttcat ctcgatgaga tcagggaaga tgtgctcagg gattatctgc
      961 tgtcgcttaa actgggaggg gtgggttatt atggggaccg tcttatggtg catatggatt
     1021 ttggtcccct tcgtgagtgg cattcaggcg atttttcgga aaatacgaag attggtgtct
     1081 ttaatgacaa aaatcgggtc agaatcacca ccgacaaatt ttattatgct ttagccgatc
     1141 cccagaattt ggtttttgag ggcatagagc cagtggcagg gcatcaaatg cagttgcaga
     1201 aatttctgcg gggacaatgg caggatgtta ccggggttgt cttgttcggt catatgtcat
     1261 cgatcgccaa cctgcagaca ctgatgtcat ctgtggtggc aacttcgcca tacggaaaat
     1321 ttcgttggca atggcgtcat ggtgaggcgt ggcagaattc gaatgagttt tatattaaaa
     1381 aatgattctg ctcatcgata actacgattc cttcacgtac aatctgtatc aatacctcat
     1441 ggaattgggt gccctggtac aggtgcatcg caacgatcaa atcacactcg atgatattgc
     1501 taccctgagg ccagctaaga tcgtgatctc gccgggtccc tgcacaccga atgaggccgg
     1561 gatttcgatg gacgtggtca aacgatttgc agggcagatc cctattttgg gggtgtgtct
     1621 tggccatcaa gccttggcgc aggcctatgg ggggagcatc attcgggccc ccaaaatcat
     1681 gcatggcaaa acgtctccca tttttcataa cggacgcggc ttatataggg ggttgccaaa
     1741 tccttttttg gcgactagat accactcgct cattgtggaa agagagtcat tgccctctct
     1801 gctggaggtc acagcctgga cggaggatca actcatcatg ggtatggggc acagggacta
     1861 tcccgtgtac ggcgtgcagt ttcatcccga gtccattttg acaacacaag ggcatgcctt
     1921 gttgagaaat tttttagatc aagtgactta acggtgtatg tccttggttg acgttttttt
     1981 tgg
//
LOCUS       DATDUN010000532         1043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_277217, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000532 DATDUN010000000
VERSION     DATDUN010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277217"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VJC18_07500"
     CDS             complement(<1..871)
                     /locus_tag="VJC18_07500"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLD45264.1"
                     /translation="MIRPIIAAALAVSFTFVNPCSQESKVQKPKSSDPVYDVTVVYPK
                     FISIPKKYHVPGTLEASEKNFVTANAAGEVEQVYVSVGDKVLKDDPLVSIASTQLMEK
                     IDIKRARIKEYQARLSAAQAGLDETGGEDRPVSLEETQFLDDEPVDEAVVEKKFGDAD
                     NATKTPKTLKALVEVLESAIDVLQKESNVLDRSLLQLSHLSPVEGTVTELLASERNRV
                     GERDKLVEVSQTNPMSVIFYLPEETASHADKNSSVKVLVTDAPDVSGEGTVYFISPNI
                     TTEVGKIEVRAHLA"
BASE COUNT          227 a          287 c          249 g          280 t
ORIGIN      
        1 tcgccaagtg agccctcacc tcgatcttgc ccacctctgt agtgatgttg ggactgatga
       61 aatacacagt gccctcgccg ctcacgtccg gcgcgtctgt gaccagcacc ttgacactgg
      121 aatttttatc ggcatggctt gccgtttctt cgggcagata aaaaataacc gacatcggat
      181 tggtttggga gacctctacc agtttatctc tttcgccaac cctgtttctt tcggacgcca
      241 gcagttcggt aactgtcccc tccaccgggc tcaggtggct caattgcaat aagctgcgat
      301 cgagcacatt ggattccttt tgcagaacat cgatggcact ttccagaacc tcgaccagag
      361 ccttaagggt tttgggggtt tttgtggcgt tatcagcatc accaaatttt ttctcgacca
      421 cagcctcatc gacaggctca tcgtccaaaa attgtgtttc ttccagtgac acaggacgat
      481 cctcaccccc cgtttcgtcc aggcccgcct gcgccgcgga cagacgtgcc tgatactctt
      541 tgatccgcgc gcgtttgatg tcgatcttct ccatgagttg tgtgctggct atactcacca
      601 aaggatcgtc tttaagcacc ttgtcgccaa cactcacgta gacttgttcc acctcacccg
      661 cggcgttggc cgtgacaaaa ttcttctcac tggcctccag cgtcccgggc acatgatatt
      721 ttttggggat cgaaatgaat ttggggtaga caacagtcac gtcgtaaacg ggatcactgc
      781 ttttgggttt ttgaacctta ctctcctgcg aacacgggtt gacgaacgtg aacgaaacgg
      841 ccaaggccgc ggcaatgatt ggtctgatca tgcgcgcaga aatagtccct ctgatagttt
      901 atagtcaagt tctggttatt attcgcagta atacaaattg tctggcaggt cctgtgatca
      961 agcccctccg attgcttttg aaacgtcgag gaccgcaccc tcaatgggag tcattttgcg
     1021 aaaaagacaa tcaggggggc att
//
LOCUS       DATDUN010000533         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_277256, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000533 DATDUN010000000
VERSION     DATDUN010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277256"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1220)
                     /locus_tag="VJC18_07505"
     CDS             complement(306..1220)
                     /locus_tag="VJC18_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HLD45265.1"
                     /translation="MNSKKTIKQFLLPVLPFLLSVLFVHVPLFYFIMSHSFDRDLSQD
                     ITDSEKVVFVDLKEMPSLPIVDIQKPSVEKVPDNPSAQGLYNSSVAKETVSRRFNKHN
                     VPLGQPAQPSAHKQSQNQQQRPSPSTQTPQSPPSSMEDQVKALMEGNKKIENEKYVSR
                     YGSSAPPPKFQAQIGSPQGGNTDDFLPNFQVGNRTYLNVLANPTIAYYVELFRKFKLA
                     WNPLPVLYRQGYYVPRGGHITVIWGFTIDQNGNLAEPVLIKGSGNGPYDNEAKRTLQV
                     SAPFTNPPPHLLNQNGVLYVGLPFIVYN"
     gene            complement(1217..2878)
                     /locus_tag="VJC18_07510"
     CDS             complement(1217..2878)
                     /locus_tag="VJC18_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HLD45266.1"
                     /translation="MKQTTPRPFKLSKTRFIRAYYTTFNILLRYLALMFFARLLDAER
                     SLALFERAHVRTAKQIIRTLLNLKGLYIKLGQTLSAMGNILPQSLTQELELLQDQVPP
                     HAFAEINERFLTDFGKTAEELFRKIDPQPIASASLGQVHVAYHKNGEKLAVKLQYPNI
                     DKLVQADLKTIKNIFAIIHFIFPGYNLKTVVSEASQVILKELDYINEAQNIETIANNF
                     KNADHILFPKVYHDLSSQKVLTLSFIEGTKITNLQEGVKISWDRKKIATDLINFYCKQ
                     VFVDGIYHADPHPGNIVITNEGKIAVFDFGAVATVSSQMKQGLTLFVEGLIKKDARMM
                     SQAIKLMGFVAKRNDEETLEKVVDYFYSKIRGIKIDSFKNLDVTKFQNLNDLIELKKM
                     DISLSDLTSLFVVPRDWIMLERTILLMSGLTAQLDDKLNPVEIVVPYVETFILGDDHK
                     RLAEILLSTSKDIIISYINLPDDLHRLAKQIRTQGIRVENKSLQKELSGIRRSLKLVA
                     IGIVTATCGILSYLFGSDGVTDIAHRLEFGFYALGVLFVLMLLRR"
BASE COUNT          732 a          605 c          832 g          795 t
ORIGIN      
        1 ctgaggacag gcgggttgta atccttgagt gattccagtt tgggcaggtc tttggaaaaa
       61 gatttgaaca gataatacag cccgcccgca ctgcaaaggg tgaccaggat cgagaaatag
      121 aagcagaatt tgatgaacca gtaaaatcgt ttcataaaag ccgggtcggt ataaacaaaa
      181 taaaaacaaa agacaaatga gtatttgctc aatggcgaaa ggcgtaaggc acaaggcgta
      241 gggcattagg aaaaattaaa cgattatttg ataacgtttt cacatcaaac ctttgaccct
      301 atgccctaat tgtacacaat aaagggcagt cccacgtaaa gaaccccgtt ttgattcaaa
      361 aggtgtggtg ggggattcgt aaagggggca gagacttgga gtgtacgttt ggcctcgtta
      421 tcataaggcc cattgcctga ccccttgatc aagactggct ccgctaaatt tccattttga
      481 tcaatcgtga aaccccaaat gaccgtgatg tggccgcctc ggggcacata gtacccctgg
      541 cgatacaaaa cgggcagggg attccaggcc agcttgaatt tgcggaacag ttccacatag
      601 taggcgattg ttggattggc gagtacattc aaataggttc ggttgcccac ctgaaaattc
      661 ggcaaaaaat catcagtgtt ccctccctgt ggtgagccaa tttgtgcttg gaattttggt
      721 ggtggcgccg atgaaccata tcgtgagacg tatttttcat tttcgatttt tttgttgccc
      781 tccattaatg ccttgacctg atcttccatc gatgagggag gcgattgtgg ggtttgtgta
      841 gagggagacg gtcgttgttg ttggttttgg gactgtttat gagcagaggg ttgagcgggc
      901 tggcccagcg ggacattgtg tttgttaaat cgtcgggaga cggtttcttt ggcgacacta
      961 gaattgtata aaccttgtgc cgaggggttg tcggggacct tttcaacaga gggtttttga
     1021 atgtcgacaa tcggcaggga aggcatctct ttaagatcca cgaaaacgac tttttctgag
     1081 tctgtgatgt cctgcgacag atcacgatca aaggaatgtg acatgataaa ataaaacagc
     1141 ggcacatgta cgaacaggac actcaagagg aaaggaagaa cggggagaag gaattgtttg
     1201 atggtttttt ttgaattcat cgccgcagca gcatcaagac aaagagaacc cccagggcat
     1261 aaaagccaaa ctcaagacgg tgagcgatgt cggtcacgcc atccgagcca aaaaggtaag
     1321 acaggatgcc gcaggtcgca gtcacaatgc cgatcgcgac cagcttgagc gagcggcgga
     1381 taccggacag ttctttttgc agggatttgt tttccacgcg gatcccctgc gtccggattt
     1441 gttttgccag cctgtgcaga tcgtcgggca ggttgatgta ggaaatgatg atgtccttgc
     1501 tggtagacaa aaggatttcg gccagtcgtt tgtggtcatc acccaggata aaggtttcga
     1561 cataggggac cacgatctcc acagggttga gcttgtcatc gagttgggcc gtcagtcccg
     1621 acatcagcaa tatggtgcgc tccagcatga tccagtctcg tggtaccaca aagagcgagg
     1681 tgaggtctga gagactgatg tccatttttt tcagttcgat caagtcattc aggttctgaa
     1741 attttgtgac atcgagattc ttgaaactgt caatcttgat gcccctgatt ttggaataaa
     1801 aatagtcgac cactttttcg agcgtctctt cgtcattgcg tttggccacg aaccccatga
     1861 gtttgatcgc ctgcgacatc atccttgcgt ctttcttgat gagcccctcc acaaacagcg
     1921 tgagcccctg tttcatctgg gaggaaacgg tggctacggc cccaaaatca aatacggcaa
     1981 tcttgccctc attggtgatc acaatgtttc ccgggtgggg gtccgcgtgg tagatgccgt
     2041 cgacgaagac ctgcttgcaa taaaaattga tgaggtcagt cgctatcttt tttctatccc
     2101 atgatatttt tacaccctcc tgaagattgg tgattttggt gccttcgatg aaagagaggg
     2161 tgaggacctt ttgacttgag agatcatgat agaccttggg aaacaggatg tggtcggcgt
     2221 ttttaaaatt gttggcgatg gtctcgatgt tttgggcctc attaatgtaa tcgagttcct
     2281 tgaggatgac ttgcgaggcc tcagagacaa ccgttttgag attgtagccc gggaaaatga
     2341 agtggatgat cgcaaaaata tttttgatgg ttttgagatc cgcctgtacg agtttgtcga
     2401 tgttggggta ttgcagtttc acggccagtt tttccccatt cttgtgataa gccacatgga
     2461 cctggccgag agaggccgac gcgatgggtt gaggatcgat ttttcgaaaa agttcttcgg
     2521 ccgttttgcc aaaatcagtc aggaagcgtt cgttgatctc ggcgaaggca tgcgggggga
     2581 cttggtcctg cagaagttcc aattcttgtg tgagactctg tggcaggata ttgcccatgg
     2641 cagagagggt ctggccgagt ttgatgtaga ggcccttgag gttcagcagt gtccggatga
     2701 tctgcttggc ggtcctcacg tgtgcgcgct caaagagggc cagactgcgt tctgcgtcca
     2761 gcagtcgtgc gaagaacatc agggcaaggt agcgcagaag gatgttaaag gttgtgtagt
     2821 aggccctgat gaagcgggtt ttggagagtt tgaaggggcg tggggttgtt tgtttcataa
     2881 cggcttgctt ttaggaaatt tttgagagaa tgtcgacggg ttcttgtgat gttcgggacg
     2941 agggcaagaa atgctttttt tcac
//
LOCUS       DATDUN010000534         2076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_441263, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000534 DATDUN010000000
VERSION     DATDUN010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441263"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VJC18_07515"
     CDS             <1..464
                     /locus_tag="VJC18_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45267.1"
                     /translation="NEVLLHELDTQAYNLSYFKRKLDLEIRRAHKGVHDVCLFYFAID
                     PLDQIITDHKIKKHIIINTTSCLLEKFPDPYIVGHVDNGQWLVIATSDFLHVGSDVPA
                     IASQLTEHLNHELSSQGLAAVAQITSRVIKDLKSFKNSDDVLETVGSDSIL"
     gene            461..1819
                     /gene="radA"
                     /locus_tag="VJC18_07520"
     CDS             461..1819
                     /gene="radA"
                     /locus_tag="VJC18_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HLD45268.1"
                     /translation="MKPKTTFFCQQCGCQAPKWLGKCPECNEWNTFVEEKVQSTVNER
                     ERSGFVTLRSDKPVTLSEISLEKMPRYETQISELDRTLGGGMVPGSLILMGGDPGIGK
                     STLILQTLDHLSLNNISVLYVTGEESKEQIKLRAQRLKVTSDLTIVTENSLDRILEHV
                     EKIKPQVLVIDSIQTVYLPHLESAPGSLSQVRESAAKLLYFSKTSGTTTILIGHVTKE
                     GAIAGPRVLEHMVDTVLYFEGENTGQFRILRTIKNRYGSTNEIGVFEMTAQGLIEVTN
                     PSQHFLREKSEHSIGSCVTAALEGTRPILTEIQSLVSSSQLANPRRTAIGVDHNRVSL
                     IVAVLEKATGLNLYNQDIYVSAVGGLKVFEPSADAAILMSLVSSYKNTCLPGHMVAMG
                     EIGLSGEIRSVSHFETRLNEAKKLGYKTVVCPSKPKLTKLDQDVEVMRFKTVSELVDR
                     LF"
BASE COUNT          566 a          567 c          467 g          476 t
ORIGIN      
        1 tcaacgaggt gctcttgcac gaattggata cccaagccta caacctttcg tacttcaaac
       61 gcaaacttga tctcgagatc agacgcgccc acaaaggagt tcatgacgtg tgtttgttct
      121 atttcgcgat tgatcctctg gaccagatca tcaccgatca caaaatcaaa aagcacatca
      181 tcatcaatac cacgagctgt ctgctcgaaa aattccctga cccttacatt gtgggacacg
      241 tagataatgg gcaatggctt gtgattgcca cgagcgattt tttgcacgtg ggcagtgatg
      301 tgcccgcgat tgccagccaa ctcacggaac acctcaatca cgaattgtcc agtcaggggc
      361 tggccgcagt cgctcaaatc acctcccgcg tcatcaagga cctcaagtcg ttcaaaaaca
      421 gtgacgatgt gcttgaaacg gtgggaagcg attcaatatt atgaaaccta aaaccacttt
      481 tttctgccaa caatgcggtt gtcaggcccc caaatggctg ggaaaatgcc ccgaatgcaa
      541 cgaatggaac acgttcgttg aggaaaaagt ccaaagcacc gtcaacgaac gcgaacgctc
      601 cggctttgtg acgctccgct ccgacaagcc cgtcaccttg agtgaaatct cactggagaa
      661 aatgccacgt tacgaaacac agatttccga actcgatcgc accctcggcg gcggcatggt
      721 ccccggttct ctcatcctta tgggcggtga tcctggcatt ggcaagtcaa cactcatttt
      781 gcaaacactc gatcatttgt cgctcaacaa catttcggtt ttgtatgtga cgggtgaaga
      841 gtccaaggaa caaatcaaac tcagggcaca acgactcaaa gtcacatcgg acctcaccat
      901 cgtcaccgaa aattcactgg accgcattct ggaacatgtc gaaaaaatca aaccacaggt
      961 cctcgtgatc gactcgatcc aaaccgttta tctgccccac ctcgaatcag ccccgggttc
     1021 cttgtctcag gtgcgtgaga gtgctgccaa acttttgtat ttttccaaaa catctggcac
     1081 caccaccatc ctgatcgggc atgtcaccaa agaaggcgcc atcgccggac cgcgcgtgct
     1141 cgaacacatg gtcgacacgg tgttgtattt cgaaggggag aacaccggac aatttcgtat
     1201 cctgcgcacc atcaaaaatc gttacggttc caccaacgag atcggtgtgt ttgagatgac
     1261 agcgcaggga ctcatcgagg tcaccaaccc cagccaacat tttctgcgcg aaaaatccga
     1321 gcacagcatc ggctcgtgtg tcacagccgc tttggagggt acgcgaccga ttctcaccga
     1381 aatccaatca ctcgtgagtt cttcacagtt ggccaacccg cgacgcactg ccatcggtgt
     1441 ggaccacaac cgtgtgtcac tcatcgtcgc ggtgctcgag aaagccacgg ggctcaatct
     1501 gtataatcag gatatttatg tgagtgccgt gggaggactg aaggtgtttg aaccctccgc
     1561 ggacgcagcc atcctcatga gtctggtgag tagctacaaa aatacctgcc tccccggcca
     1621 catggtggct atgggtgaaa tcgggctcag cggtgagatc cgttcggtga gtcatttcga
     1681 aacccggcta aacgaggcca agaaactggg ctacaaaacc gtggtctgtc ccagcaaacc
     1741 aaaactgaca aagcttgacc aagacgtcga ggtcatgaga tttaaaaccg tctcggaact
     1801 ggtggatcgc ttgttttgat ccgaaaacaa tttcctgata acgatcacta cacaagagtt
     1861 gtattctcac ccaccccacc gcggaaacca attcccacag cgacataacc ttcaaaaacc
     1921 agcaaccata tcgggatatt acaaagatgt ttgcttttaa tctaataatt cagtcattct
     1981 aaggatttac aaattttgtg tccttatgac ctgtcaacct taaactgaag gaaatttgcc
     2041 aaggggatat ctcgtcaata ttactgatct atttta
//
LOCUS       DATDUN010000535         1118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_315045, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000535 DATDUN010000000
VERSION     DATDUN010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315045"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="VJC18_07525"
     CDS             complement(<1..127)
                     /locus_tag="VJC18_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLD45269.1"
                     /translation="MANEKTVCVVGAGLAGSECAYQLAKGGIKVVLVEMRPQKNTP"
     gene            264..>1118
                     /locus_tag="VJC18_07530"
     CDS             264..>1118
                     /locus_tag="VJC18_07530"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HLD45270.1"
                     /translation="MSILVIVESPTKAKTIKKFLPKGYDVTSCVGHVRDLPKSAKDIP
                     AQYRSQEWTKLGVDVDNDFQPLYLIPNNKKKVIKELKDRLKDAKEIILATDEDREGES
                     ISWHLKEVLNPKIPIKRMVFHEITKEAIEEALHQFRDIDLSLVRAQETRRILDRLVGY
                     TISPLLWKKITYGLSAGRVQSVAVRLIVQREMERRHFRQGTYWDLKAKLKHHHDVFDA
                     MMISWANQRLATGKDFDETTGKLAQGKDVLLLDQKKAEEFKARLLKASWQVVGVDEKP
                     SLRRPYPPF"
BASE COUNT          316 a          308 c          249 g          245 t
ORIGIN      
        1 ccggcgtgtt tttttgggga cgcatttcta ccagaacaac tttgatgccc ccctttgcca
       61 attgataggc acattcggat ccagccaaac ccgcccccac cacacaaact gttttttcat
      121 ttgccatgaa gtctcctcat aagaaagccc tgcacaccgg tcaacatatc aatgaaatca
      181 tttcagaatt tatcatatcc caacttccct ttccaaaaaa cagcttgatc ttaggcatca
      241 ttttctgcca aacgagcgcc ctcatgagta tattagtcat agtcgaatca ccaaccaagg
      301 ccaagaccat caaaaaattt ctccccaagg ggtatgatgt gacctcttgt gtggggcatg
      361 ttcgcgatct gcccaaatcg gccaaagaca tccctgccca ataccgatca caagaatgga
      421 ccaagctcgg tgtggacgtg gacaatgact ttcaaccgtt gtacctgatt cccaataata
      481 aaaaaaaggt catcaaggaa ctcaaagata gactcaaaga cgctaaagaa atcattctcg
      541 caaccgacga agaccgcgag ggagagagca tcagctggca tcttaaagaa gtgctcaacc
      601 ccaagatccc catcaagcgc atggtttttc acgaaatcac caaggaggcc attgaggagg
      661 ctctgcacca atttcgcgat atcgacttga gcctggttcg tgcgcaggaa acgcgacgta
      721 tattagaccg gctggtgggg tacaccatct cacccctatt gtggaaaaaa atcacctacg
      781 ggttgtctgc gggacgcgtg caatccgtgg ccgtgaggct cattgtgcag cgcgagatgg
      841 aacgccgtca ttttcgccaa ggcacgtatt gggacctcaa ggcaaagctc aaacatcatc
      901 acgatgtttt tgacgccatg atgatttcct gggcgaatca aaggctcgcc accggaaaag
      961 attttgatga aacaaccggc aaactcgctc aaggcaagga cgtcctcctg ctcgaccaga
     1021 aaaaggccga agagttcaag gcccgtctcc tcaaggcctc gtggcaagtg gtgggtgttg
     1081 acgaaaagcc ctccctcaga cgaccgtacc caccgttc
//
LOCUS       DATDUN010000536         2739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_193843, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000536 DATDUN010000000
VERSION     DATDUN010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193843"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1723
                     /locus_tag="VJC18_07535"
     CDS             170..1723
                     /locus_tag="VJC18_07535"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLD45271.1"
                     /translation="MEDHNHKTKTSSNVIDLKTGERHFELNQRIKMGNYKPNEELIQS
                     TARIKKQRDVIKDRFQKMEVAKERVTKNVYDKVKRDYLLQLQTINELLDEKKGELKKE
                     IKELYLFREKLSIEISRHREILEEAEFRLYLGEFLQNQYQEVENFETKEIEKLETELS
                     QISQYIQTHEELFDPEDLGLSPPQPATDVTVTKTVFRPEQKQAGTTTATQPAPRQPAD
                     PSREARPTAPVTRQPEKRSPAIEPPKTTSSPVKPQTMQPEKTQSEEITIDPSEFEDLF
                     LDDDKPETQPEPADGIDKPEAPAIEQTSSSITVDTNLPTTNSDTDYFAQEKVNEASFT
                     MKSETLNEVINAAAAEEPEQEITEQKQVRQNADIKTESKITKPTQPEPVSTIEEDSIS
                     DILNSLPVEEQPKPKEKLAPSNNDKFDSESVYYLKLVGGEHEQKEFPLKENIAIGRSP
                     SSDIVLKAPKVSRQHAAINMYNNQYIIIDLKSSNGVYVNGTKIDECVLNSGDEISIGG
                     YRFIFTDKV"
     gene            1765..>2739
                     /gene="ispG"
                     /locus_tag="VJC18_07540"
     CDS             1765..>2739
                     /gene="ispG"
                     /locus_tag="VJC18_07540"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HLD45272.1"
                     /translation="MTQSRYQRRPTTPVFVGQLGIGGNFPIRVQSMLSVDTKKTVEAV
                     AQIQALINAGCELVRLTVPTHDDVKALAEIRRVMQQKKLRVPLVADVHFSPSIALEVV
                     PHVEKVRINPGNFVDKTTNDKARYHPAHYQNDLDRLEEKFVPLVEQCRSHGVAMRLGT
                     NHGSLSGRIMSRFGDTPEGMVESALDFVRLAHKHNYHDIILSMKASNVRVMVQAYRLL
                     VVKLDELQLPLPFHLGVTEAGDAEEGRIKSSAGIGTLLEDGIGDTIRVSLTEDPVNEI
                     AVAHLIATPYNQRFYSASFSTAQTSVQQTPPFSASSFKRRTSSAHIIGL"
BASE COUNT          825 a          788 c          583 g          543 t
ORIGIN      
        1 ctaaagtcac ttgttatcgg gttacgaacc ccttctgggc atcaatcagg tacttctctg
       61 atattcatac agaatattga catttcacgc aagaaatcct tgcataatag ttctcttgca
      121 ttattttggg attgaatcgt aaccttcaat cggattatat aaaaatacta tggaagatca
      181 caatcataag acgaaaacat cctcaaatgt tatcgattta aaaactggcg agcgccactt
      241 cgaactcaat caaaggatca aaatgggcaa ttataaacca aacgaagaac tcatccaatc
      301 gacggcgcgc atcaaaaaac aaagggatgt catcaaggat cgtttccaaa aaatggaagt
      361 cgccaaagag cgtgtcacca aaaatgtgta cgacaaggtc aagcgcgatt atttgctcca
      421 actgcaaacc atcaatgaac tgctcgacga aaaaaaaggt gaactcaaaa aagaaatcaa
      481 ggagctttat ctcttccgcg agaagctctc catcgagatc agccgacatc gagaaattct
      541 cgaagaagcc gagttccgtc tgtacctggg cgaatttctg cagaaccagt atcaggaagt
      601 cgaaaatttc gaaacaaagg aaatcgaaaa actggaaaca gagttgtctc aaatttccca
      661 gtacatccaa acccacgagg agctgttcga tcctgaggat cttggcctgt cgcctcccca
      721 accagccaca gacgtgaccg tgaccaaaac tgttttcaga cctgaacaaa aacaagcagg
      781 gaccacgacg gcaacacaac cagccccaag acaaccagcc gatccttcca gagaagcgcg
      841 tcccactgcc cccgtcaccc gacaaccaga aaagcgatct cctgcgattg agcctcccaa
      901 aacaacctcc agtcccgtta agccacaaac catgcagcca gagaaaaccc agtccgagga
      961 aatcaccatt gatcccagcg agtttgaaga tctgtttctg gacgacgaca aacccgagac
     1021 acaaccggag ccagccgatg gcattgacaa gcctgaggcc cctgctatcg aacagaccag
     1081 tagcagcatc actgttgaca cgaacctgcc gacaacaaat tccgataccg actattttgc
     1141 tcaggaaaaa gtcaacgagg cttccttcac catgaaatcc gaaaccttaa acgaagtcat
     1201 caacgctgcc gctgcggaag aacccgaaca ggaaatcaca gagcaaaaac aggttcggca
     1261 aaacgccgac atcaagactg agagcaaaat caccaagccc acccagccgg aaccggttag
     1321 caccatcgaa gaagattcca tcagcgacat cctcaattct ctgcccgtcg aagaacagcc
     1381 caagcccaag gaaaaactgg cccccagcaa caatgacaag tttgattcgg aatcggtgta
     1441 ttacctcaag ctcgtaggcg gcgaacacga acaaaaggag tttcccctca aggaaaacat
     1501 cgccatcggg cgatccccct ccagtgacat cgtgctcaag gcccccaagg tgtcgcgcca
     1561 gcacgccgcg atcaacatgt acaacaatca atacatcatc attgacctca agagttcaaa
     1621 cggcgtgtac gtcaacggca ccaagattga tgagtgtgtg ttgaattccg gcgacgaaat
     1681 ttccattggc ggttatcgtt ttatttttac cgacaaagtt tagggttccg ctcaaaaccc
     1741 ggcaaacacc cgaaaaaaaa cagcatgaca caatcgcgct atcaacgcag acccacaacg
     1801 ccagtatttg tcggccaatt gggcattggc ggaaattttc ccatccgcgt gcagagcatg
     1861 ctcagcgtcg acaccaaaaa aaccgtcgag gctgtggctc aaattcaagc gctcatcaac
     1921 gccggctgtg aactggtcag actgaccgtc cccactcacg atgatgtgaa ggccttggcc
     1981 gagatccgca gggtcatgca acaaaaaaaa ctgcgcgtcc ctctcgtcgc tgatgtgcat
     2041 ttttcaccct ccattgcttt ggaggtggtg cctcatgttg agaaggtcag gatcaacccc
     2101 ggcaatttcg tggacaagac cacaaatgac aaagcacgct atcatcccgc acattaccaa
     2161 aacgatctcg acagactcga agaaaaattt gttccccttg tcgaacaatg ccgctctcat
     2221 ggcgtcgcca tgcgcctcgg gaccaaccat ggttcgctct caggccgcat catgagtcgt
     2281 tttggagaca cgcccgaagg catggtggaa tccgcgctcg attttgtgcg cctcgctcac
     2341 aaacacaatt accacgacat catcctatcc atgaaggcct ccaacgtccg tgtgatggtg
     2401 caggcctacc gcctgttggt cgtaaaactt gatgaactcc aactccccct gccctttcat
     2461 ttgggggtca ccgaggccgg cgacgctgag gagggacgca tcaaatcctc tgcggggatc
     2521 ggcacccttt tggaagatgg gattggcgac accatccgcg tatcactgac cgaagaccct
     2581 gtgaacgaga tcgccgtcgc ccacctgatc gcgactccat acaatcaaag attttacagc
     2641 gcgtcttttt caacagcaca aaccagcgtt cagcaaacac caccattttc ggcatcctcc
     2701 ttcaaacgcc gaaccagttc agcacacatc atcggcctc
//
LOCUS       DATDUN010000537         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_193871, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000537 DATDUN010000000
VERSION     DATDUN010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193871"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /gene="dnaG"
                     /locus_tag="VJC18_07545"
     CDS             <1..1756
                     /gene="dnaG"
                     /locus_tag="VJC18_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HLD45273.1"
                     /translation="RSTIHDPRSTIMPRIPQHIIDQLIADTDIVDIISEYVSLTKAGS
                     NYKARCPFHQEKTASFMVSPDKQIFHCFGCGAGGNVVSFLMKHDQSEFIDVIKKLANR
                     LNIKLEFDEKKSTEDDTFELLYKVNAYAHWYFRTQGQQSQKVKTYIQNRGLNPETTAL
                     YDLGYAPDGFENLVPFFKTKKIPLSLASQLGLVKKGNRDNLYDFFRDRLMFPIHNVHG
                     KIVGFGGRALQSDQEAKYLNSAESPIYHKNKELYGLYQAKKDIAQKKQAVVVEGYIDV
                     LAAHQLGISNTVAPLGTSLTLDQVKKLKRFAPEIILMFDGDMAGVNAAQKAITTCFSA
                     GIHPKLVILPNNLDPGDYLTNPQPSQSLTKAVAEAIFAMDWLLSTRIKKVTHETGSRA
                     QIIREIAGWLGQIPDAIDQMEYKKKFCQYFDVTPNDIEKFVDITYESDSGVRPDEKAP
                     PLEALLILCYLDDSALFPEGGLVALAKNFEDSRLRDLALHVESFLKNHETFEPAQAIL
                     DLPEALATILSKILLFLDDKSGSLDTAGCINKFQAMQKKKRLKEITSQILEAEVTKDT
                     ERKIALLKEKQDLLANRQ"
     gene            1785..>2496
                     /locus_tag="VJC18_07550"
     CDS             1785..>2496
                     /locus_tag="VJC18_07550"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HLD45274.1"
                     /translation="MSKKKTNPKHKALAPKNAGVPKHKPIAKTAAKHKEQSPQAKQIK
                     PLKAEAPATNAHQKTHVAVVKKANATPSQKQPDKKKSPVANFVPLDLSKTELDIEERL
                     QQMLEVGRERGFLTYEELNINIPENVTNANRLDEILCLIEEVNIQIVEDPSDFKPEKI
                     KKVSKAVVEESTADAPFTSSSAPQKMFTTMEGAGSSNDPVRMYLRKMGSVSLLTREGE
                     VEVAKRIEHWEHKVFEIIL"
BASE COUNT          767 a          658 c          544 g          527 t
ORIGIN      
        1 acgatccacg atccacgatc cacgatccac gatcatgccc cgcataccac aacacatcat
       61 cgaccaactc atcgccgaca ccgacatcgt cgatatcatt tcggaatatg tttccctcac
      121 caaggcaggg tccaattaca aagcgcgctg tccgtttcac caggaaaaaa cagcgtcgtt
      181 catggtgagt cccgacaaac agatctttca ttgttttggt tgcggggcag gcgggaacgt
      241 ggtgagtttt ctgatgaaac acgatcagag cgagttcatc gacgtcatta aaaaactggc
      301 caaccgtctc aacatcaaac ttgagtttga cgaaaaaaaa tccaccgaag acgacacctt
      361 cgaactgctg tacaaagtca atgcgtatgc ccactggtat tttcgcacgc aagggcaaca
      421 gtctcaaaag gtcaaaacgt acatacaaaa cagaggcctt aaccccgaaa ccacggccct
      481 ctacgacttg ggttacgccc ccgatggatt cgaaaatctg gtcccctttt tcaaaacaaa
      541 aaaaattccg ctgtcactcg ccagccagtt gggcctcgtc aagaaaggca atcgcgataa
      601 cctctacgat tttttcaggg accgcctgat gtttcccatt cacaacgtgc acggaaaaat
      661 cgtgggtttt ggaggacgag ccctgcaatc tgaccaagag gccaaatacc ttaactccgc
      721 cgaaagcccc atctatcaca aaaataagga gttgtatggt ctgtaccagg ccaaaaagga
      781 cattgcccaa aaaaaacagg ccgtggtggt ggaaggctac atcgatgttc tggccgcaca
      841 ccagttgggc atcagcaata cagtcgcccc attgggcact agcctcactc tggatcaggt
      901 caaaaaactc aaacgctttg cccccgaaat catcctgatg ttcgatggag acatggccgg
      961 cgtcaacgca gcccaaaagg ccatcacgac ctgtttttcg gccggaatcc atcccaagtt
     1021 ggtgatttta ccgaataatt tagatcctgg cgactatttg acaaacccgc aaccatcgca
     1081 aagtctcacc aaagctgttg cggaggcgat ttttgcgatg gactggcttc tttcgacccg
     1141 aattaaaaaa gtcacacatg agactggttc acgcgctcaa atcatccgtg aaatcgcggg
     1201 atggttggga caaatcccag acgccatcga tcagatggaa tataaaaaaa agttctgtca
     1261 gtatttcgat gtcaccccga acgatataga aaaatttgtt gatattacat atgaatcaga
     1321 tagtggtgtc cgtcctgatg aaaaggcccc cccactcgag gccctgctca tcctgtgtta
     1381 cttggatgac tcagccttgt ttccagaagg gggacttgtg gctctggcca aaaacttcga
     1441 ggatagccgc ttgagggatt tggccctgca cgtggaaagt tttttaaaaa accacgaaac
     1501 atttgaaccg gctcaagcga tactcgatct gcctgaggcc ttggcaacaa tactgagcaa
     1561 aattctactt tttttggatg acaaaagcgg ttcccttgat acggcaggct gcatcaacaa
     1621 atttcaggcg atgcaaaaga aaaaacgctt aaaggagatc acctcacaga ttctcgaagc
     1681 agaggtgacc aaggatacag aacgcaagat cgctttattg aaagaaaaac aagatttgct
     1741 ggcaaaccgg caataacaga tttctccaca ttacagaggg tgtcatgagc aagaaaaaaa
     1801 ccaacccaaa gcacaaagcc ctagcaccta aaaatgcggg tgtgcccaag cataagccaa
     1861 tagccaaaac tgcggccaaa cacaaagaac aatccccaca ggcaaaacag atcaagcctc
     1921 tcaaggcgga agctccagcg acaaacgccc atcagaagac acatgttgct gtcgtaaaga
     1981 aggcaaacgc taccccctca caaaaacagc cggacaaaaa aaaatcaccg gttgccaatt
     2041 tcgttcccct cgatctttcc aaaaccgaac tcgacatcga ggaacgtctc caacaaatgc
     2101 tcgaagtggg gcgtgaacgg ggttttctca cctacgagga gctcaatatc aacattcccg
     2161 aaaacgtcac taacgccaac cggctcgatg aaattttatg tctcatcgag gaagtgaaca
     2221 ttcaaattgt cgaagaccca tccgatttta aaccagaaaa aatcaagaaa gtcagcaagg
     2281 ctgtcgttga ggaatccaca gcagacgccc ctttcacatc gtcatctgct ccccaaaaaa
     2341 tgttcaccac aatggaaggc gctggttcct ccaacgatcc cgtccggatg tacttgcgca
     2401 aaatgggctc tgtgtctctg ctcacacgcg agggcgaagt ggaagtggcc aagagaatcg
     2461 aacattggga gcacaaagtt tttgagatca ttctgg
//
LOCUS       DATDUN010000538         8223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_28468, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000538 DATDUN010000000
VERSION     DATDUN010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28468"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..2013
                     /locus_tag="VJC18_07555"
     CDS             259..2013
                     /locus_tag="VJC18_07555"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HLD45275.1"
                     /translation="MKFSKTLIPTLREAPGDAEVISHQLLIRAGFIKKVAAGIYEFLP
                     LGLKSLRKVEQIIREEMDRAGSLEVFMPHLVPAELWQESKRWFKYGPELLRIKDRHEH
                     EYCFGPTHEEVVCDLVKQSIQSYKSLPVTLYQIQTKFRDEIRPRFGLMRGREFLMKDA
                     YSFHLTPEDLDHEYQNMYQTYQRIFARCGLASRAVEADTGSIGGASSHEFMVLASTGE
                     DVIAACNTCQYAANLEKAFFANLPLTAQPTQEVSQDVVTPNQKTIEEVSSYLNVRPSQ
                     MIKTLVYVVGEKTVIVCLAGDRDVSEAKLKALTKSAEVRFATEEEIMLLTGVPIGFLG
                     PQGLLDRLAQRGASDKVTLFYDRSLIGVHNGVTGANKVDHHKININIERDLGLSQDPR
                     SAKYVDLSTVIEGDACPKCQAGKLTLMRGIEVGHIFKLGDVYSQPMGVKVLDKDGQEK
                     VAIMGCYGVGVGRTLAASIEQNHDDKGIIWPRALAPYEVYLMNLDGSDEIKNAAQNIY
                     NELTQNGVTVLWDDRDDRAGVKFNDADLIGIPLQIMVGKKGLAREICEYKIRRDGTRG
                     ELPYAQVVSGVKDLLVKL"
     gene            2129..3082
                     /locus_tag="VJC18_07560"
     CDS             2129..3082
                     /locus_tag="VJC18_07560"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HLD45276.1"
                     /translation="MRKHLLEYLDKGESGTVRGLRIMVEYFYALEQLRVVLRPDVKVV
                     SVFGSARLKPDDREYKTAKLLGRALYESGFAVVTGASNGVMQAANEGVYDGILSDLQQ
                     VYKNKTLDFIKETAEFKRKLGDYSLGLKISLPFEKEPNPYLGVMATFHYFMVRKFFFA
                     KISSAFIACEGGWGTRDELFEIMTLVQTGKAPLMPIVYISPTPMHMQNDINYAVKKGH
                     IAPEDKKLVNVVTSFKQAIRILNRFYRYVNYISYDKMPHITVYLKKDLSEAQKTKLQK
                     TVVEKYPSVFTGGVRFFKNRFELRSYVHKSYGILRCLIDSL"
     gene            3259..5142
                     /locus_tag="VJC18_07565"
     CDS             3259..5142
                     /locus_tag="VJC18_07565"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HLD45277.1"
                     /translation="MNQHLSKIQTPAEVKKLSLKELELLAETLRSDIVQHTSTNGGHV
                     APSLGCVDIIVALHRVFETPEDTFVFDVGHQAYAHKMLTGRRFEFYKLRRENGLSGFT
                     TPSESVHDGFGSGHASTSISVALGILEGKRKLGDQHHVIAILGDGSLTGGLAFEALNH
                     AGELKRNLIVVLNDNQMSIDRNVGGLKDSLGTPDARDYFGALGFDYWGPGNGHDIPRM
                     VRLFEKARKHRRPLVLHVRTQKGKGYQPAIADQTNFHGCGPFDITTGSALKSPTSKVK
                     YQDLFAQTLIEMASIDDKIVAITAAMPSGTSLNRFKNIHPDKFYDVGIAEAHAVTFAC
                     GLATQGIKPFVCIYSTFMQRAYDQLVHDAGINNLPIKICMDRGGLVGDDGVTHQGVFD
                     FAFLRTIPNFVIMAPKDEVELQRMMETARLYQRGPISLRFPRGEVVGVPLPSQIVPLP
                     IGKAEHLYGDAVGDVLLCAIGSTVRDAVTAAKILEEQHSLSVGVINLRFAKPLDEGMI
                     TSLARNFRVLLTVEDGVVKGGVGSAILECLTENHMRVPVKCLGVPDAFVRHASQARQR
                     EFCHLDAQSIVREARELLVEATSCPLKQVANKQHNVYHFKEKTPSLVAITPFAVAKTQ
                     DSN"
     gene            5173..6780
                     /locus_tag="VJC18_07570"
     CDS             5173..6780
                     /locus_tag="VJC18_07570"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HLD45278.1"
                     /translation="MLRKKIITTLFLFFISSQTEARLLDTDWDEQLAFDQLVSCGLVE
                     KPFYGQRPWTHQTIIRSLESVKSFSGHSACSQEVVDGLLRNFEDYRKTLSSSQPNDQS
                     KIHIKPIEGISFSYNYLSGGSLSYVNNNRGTVDAIANTFLENTAQKVRGEGHNVLFSS
                     AHYFSWYGQDLYLEPLLGVLGSNDLLRVDDAVVTWQQAYAHFNKWRMDMIVGRSPVLW
                     GQGEHGGLLFTDNARPLDVIQLTNAQPVRFPWLFGRLGVFKFSFVFGTLGPEQFFPHT
                     LFSGLSLGYKPASFLEFNLSHVLQFGGEGAPDLGFTTGLREFFGFIPGISQTAVRGGN
                     KITGFNLRFFADRLMGLQGYLEYAMDDSNLSGGSAALKKHFTANSSYLVGLFFTRPFG
                     LTVDSLRVEMTRFGQIAYRHSLYDGGWTINQHIIGHPSGPDSYSARVAWEHDWSTFRD
                     RSKVFFQWTSRDSDTYQVSADGYTETVLNEGPSEQRYAAMAEHAFDWMRLQWKVGAGF
                     EIIQNYRYVEGNRSGTLLLSSQINKTF"
     gene            6862..8151
                     /locus_tag="VJC18_07575"
     CDS             6862..8151
                     /locus_tag="VJC18_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HLD45279.1"
                     /translation="MFASKRIVSIVGLGYVGLPLAVAFGKLGETIGFDVNEKRVLELK
                     NGHDKTLETSDVELKGTNIFYTSNPEDLKKANFHIVAVPTPINSAKQPDLVPLYMASD
                     AVGSVLKKGDVVVYESTVYPGVTEEECVPILEHVSGLKAGVDFKVGYSPERVNPGDKE
                     RTLEKITKIVSGQDDETLKIVAQTYGQIIKAGVHLVSSIKVAEAAKVIENTQRDLNIA
                     LMNELAIIFEHMGIDTNEVIDAAGTKWNFLKFRPGLVGGHCIGVDPYYLTHKAEKLGY
                     HPQVILAGRRINDGMGKFIAGQTVKQMIRAGHNILNNTVTMLGITFKEDCPDIRNSKV
                     VDIINELKEFGLNVQIHDPVADVEDVKREYGVELTPYNELKPAAAVVVAVAHEQYKKL
                     LPADLQKLTVPQAVFIDVKNVYNPKALLEMGFKVWRL"
BASE COUNT         2045 a         1867 c         2248 g         2063 t
ORIGIN      
        1 atcgaagtat attgtcccgg tgtaccgaag ggcagtgtca gtcctattga gccggaggac
       61 tcatcagtcc tttggctgag gctgacacgc tgggatccag aatttataga atcatggatt
      121 cctgcgttcg caggaatgac atcatgaatt tcgatccacg attcacgatc cacgatccac
      181 gattcacgat ccgcgattca cgattcacga ttcacgaaac acttgccttt acaggtcatt
      241 ccccttattt atccgcgcat gaaattttcc aaaacattga ttcccacatt gagggaggcc
      301 ccgggcgatg ccgaggtgat ctcccatcaa ctgctcatca gggctggctt catcaagaag
      361 gtcgcggcgg gcatttacga atttttaccc ttgggcctca aaagcctcag gaaggtcgaa
      421 cagattattc gcgaagaaat ggatcgcgcg ggcagtctcg aggtgtttat gccgcatctg
      481 gttcccgctg aactttggca ggagagcaaa cggtggttca agtatggtcc cgagctcctg
      541 cgcatcaaag atcggcatga gcacgagtat tgttttggtc ctactcacga ggaagtggtg
      601 tgcgatctgg tcaaacaatc catccagtcg tacaaatcgt tgccggtcac cctgtatcag
      661 attcaaacaa aatttcgtga tgagatccgt ccccgttttg gcctcatgcg cggtcgcgaa
      721 tttctgatga aggacgcgta ttcttttcac ctcacgcccg aagacctcga tcatgaatac
      781 caaaacatgt accagaccta tcaaaggatc ttcgcgcgtt gtgggttggc cagtcgcgcc
      841 gttgaggcgg atacaggttc catcggtggt gcctccagtc acgaattcat ggtgctggcc
      901 agtacgggcg aagatgtgat tgccgcgtgc aatacctgcc agtacgcggc gaatcttgag
      961 aaggcctttt ttgccaacct gcctctgacg gctcaaccca cgcaggaagt ctcccaagat
     1021 gtggtcacgc ctaatcagaa gaccattgaa gaggtttcgt cttatctaaa tgtcaggcca
     1081 tcgcagatga tcaaaaccct ggtgtatgtg gtgggtgaga aaacagtgat tgtctgtttg
     1141 gctggtgatc gcgatgtgag tgaggccaaa ctcaaggcat tgaccaaatc tgccgaggtg
     1201 cgttttgcca cagaagaaga aatcatgttg ttgacggggg ttcccattgg tttcttggga
     1261 ccgcagggac ttttggatcg cctggcacag agaggtgcga gcgacaaagt gactctgttt
     1321 tatgaccgct ccctgatagg agtgcacaac ggggtgacgg gagcgaataa ggtcgatcac
     1381 cacaaaatca acatcaacat cgaacgcgat ctgggtctgt cacaagatcc gcgatctgca
     1441 aaatatgtgg acctctcgac tgttatcgag ggtgatgcct gtcccaagtg ccaagctgga
     1501 aaactcaccc tgatgcgtgg catcgaggtg gggcacatct tcaaattggg tgatgtgtat
     1561 tcccaaccca tgggggtgaa ggtgctcgac aaggacggac aggagaaggt ggccatcatg
     1621 ggttgttacg gtgttggcgt ggggcgaacg ttggcggcca gcatcgagca gaatcacgat
     1681 gacaaaggca tcatctggcc gcgcgcgttg gcgccctacg aggtttatct gatgaacctc
     1741 gatggttcag atgaaatcaa gaacgccgcg cagaatatat ataatgagtt gacgcaaaac
     1801 ggcgtgacgg tattgtggga tgatcgtgat gatcgtgccg gggtcaagtt caatgatgcg
     1861 gacctcattg gcattccctt acagatcatg gtgggcaaaa aaggcctggc ccgggaaatc
     1921 tgcgaataca aaatccgtcg cgatggaacg cggggagaat tgccttatgc acaagttgtg
     1981 agtggggtga aagatttgtt ggtaaaacta taatgcgtgt tcaggtgttc acgtgttcac
     2041 gtgttcaggt catggggcct ctcgtgagca cgcatgccct ccgaagctcg tagagcgtag
     2101 gggggtaaac acgtaaacac gagaaataat gcgcaaacat ctactcgaat atctggacaa
     2161 aggcgagagc gggaccgttc gcgggcttcg catcatggtg gaatattttt acgccctcga
     2221 gcaactcagg gtagtcctca gacccgatgt gaaggtcgtt tctgtgttcg gttccgccag
     2281 actcaaacct gatgaccgcg aatacaaaac ggccaagctt ttgggccgcg ccctttacga
     2341 gagtggtttt gccgtagtga caggggccag caatggcgtg atgcaggcgg ccaacgaggg
     2401 ggtgtacgac ggcatcttaa gcgatttgca acaggtttat aaaaacaaaa cattggattt
     2461 tatcaaggag acggccgaat ttaaaagaaa gcttggcgat tattctctgg gtctcaaaat
     2521 atctctgcct ttcgagaagg aacccaaccc ctatctgggg gtgatggcca cgtttcatta
     2581 tttcatggtg cgcaaatttt tctttgccaa gatttccagc gctttcatcg cgtgtgaggg
     2641 cgggtggggg actcgcgacg agctcttcga gatcatgact cttgtgcaga cgggcaaggc
     2701 cccgctcatg ccgatcgtct atatttctcc gacccccatg cacatgcaga acgacatcaa
     2761 ttacgcggtg aagaaggggc acatcgctcc cgaggacaaa aaactcgtga acgtggtgac
     2821 cagctttaag caggcgatca ggattctgaa ccgtttttac cgttatgtga actacatcag
     2881 ttacgacaaa atgccgcaca ttacggtgta tctcaaaaaa gatctttctg aggcgcaaaa
     2941 gaccaaatta caaaaaacgg ttgtagagaa gtacccctcc gtttttacgg gtggggtgag
     3001 gtttttcaag aacaggttcg aactcaggtc ttacgtgcac aagtcctatg gcatcctgcg
     3061 ctgtctcatc gattcgttat gacttttgtc atgaattaaa taattaacat ttaaaatcaa
     3121 acagatagat tcttggttca agaaatcagt gacagttatt gtataagcag gtatgaggcg
     3181 cgcccttgac gaagccttgt tttgctgaat gatcttttat cgcacaactt ttttgatcgg
     3241 gttgctgtgt cggaaaatat gaatcaacac ctctccaaaa tccaaacacc agcagaggtc
     3301 aagaagttgt ccctcaagga acttgagctc ttggcagaaa cgctccggag tgacatcgtc
     3361 cagcacacgt ccaccaatgg tgggcacgtg gcaccgtctc tgggttgtgt cgatatcatt
     3421 gtggccctac accgggtgtt cgaaaccccc gaagacacgt ttgtttttga cgtggggcac
     3481 caggcctatg cgcataaaat gctgacgggc agacggtttg agttttataa actcagaaga
     3541 gaaaacgggt tgtccggatt taccacacca tcagaatcag tgcatgatgg ttttggatcg
     3601 gggcacgcgt ccacgtccat ctctgtggcg ttgggcatcc tggagggcaa aagaaaattg
     3661 ggagatcaac accatgtcat agcgattttg ggggacggtt cgttgaccgg tggtttggct
     3721 tttgaagcgc tgaatcacgc cggggaactc aaacgcaacc tcattgtggt gctcaatgat
     3781 aatcaaatga gcatcgatcg taacgtcggt gggctcaagg attcgttggg gacgcctgat
     3841 gccagggatt attttggtgc cttgggtttt gattattggg ggcccggtaa tgggcacgat
     3901 atccctcgca tggtccgcct gttcgaaaag gccagaaagc acagacgtcc tttggtgctt
     3961 catgtcagga ctcagaaagg caaggggtat cagccggcaa tagctgatca gaccaacttt
     4021 cacggttgtg gtcccttcga catcacaacg ggtagcgcgc ttaagtcgcc aacgtcgaaa
     4081 gtgaagtatc aggatctgtt tgcccagaca ctcatcgaaa tggccagcat tgatgacaag
     4141 attgtggcca tcacagcggc gatgcccagc ggcacgtcac tgaaccggtt caaaaacatt
     4201 catcccgaca agttctacga tgtgggcatc gccgaagccc acgcggtaac cttcgcttgt
     4261 ggcttggcca cccagggcat caaaccgttt gtgtgtatct attcaacctt catgcagagg
     4321 gcctacgacc aacttgtgca cgatgcgggc ataaataatt tacctatcaa gatatgcatg
     4381 gatcgtggtg ggttggtggg agacgacggt gtgacgcacc aaggggtttt cgatttcgct
     4441 ttcctgcgca cgatccccaa ttttgtgatc atggcaccca aggacgaggt cgagctgcaa
     4501 cgcatgatgg aaacggcacg cttgtatcaa cgggggccca tcagtcttcg ctttccacgg
     4561 ggagaagtgg ttggtgtgcc tctgccgtca caaatagtgc cactgcccat tggaaaggct
     4621 gaacatcttt acggtgacgc agtgggcgat gtgttgctct gtgccattgg gagcactgta
     4681 cgcgatgcgg tcacagcggc aaagattctt gaggaacagc actcgttgtc ggtcggcgtg
     4741 atcaatctgc gttttgccaa accactggat gagggcatga tcacctcatt ggccagaaac
     4801 tttagggttc tgttgactgt ggaagatggc gtggtcaagg gcggggtggg ttctgcaatc
     4861 ctggaatgtc tgacagaaaa tcacatgcgc gtaccagtca agtgtctggg cgttcctgat
     4921 gcctttgttc gccatgcctc gcaggccagg cagagagagt tctgtcactt ggatgcgcag
     4981 tccatcgtga gagaggccag ggagcttttg gtcgaggcaa ccagttgccc cctaaaacaa
     5041 gttgccaaca aacagcacaa tgtttaccat ttcaaggaaa aaacacctag tctcgttgcc
     5101 atcacgcctt tcgcagttgc caagacacaa gattcaaatt gattgtttca gttaattggt
     5161 taaaagaacg atgtgcttcg caaaaaaatc atcacaacat tgtttctttt tttcatctca
     5221 tcacaaaccg aggccagact tctcgacact gactgggacg aacagttggc attcgaccag
     5281 cttgtttcct gcggtctggt cgaaaaacct ttttacggac agcgcccctg gacgcaccag
     5341 accatcatca ggtctttgga gtcggtaaaa tctttttctg gtcacagtgc gtgttctcaa
     5401 gaggtggtgg acgggctttt gcggaatttt gaagactatc gcaaaacact atcttcatct
     5461 caaccaaacg atcaaagtaa aatacacatc aaaccaattg agggaatttc tttttcgtac
     5521 aattatctct caggtggaag tttatcgtat gtgaacaata acagggggac tgtagatgcg
     5581 atcgcgaata cctttttgga gaacacggca caaaaagtga ggggcgaagg tcacaatgtg
     5641 ttgttctcgt ctgctcatta tttttcgtgg tatgggcagg atttgtattt ggagccgttg
     5701 ctgggtgtgt tgggttcaaa cgatcttttg cgcgttgacg atgccgtagt tacctggcaa
     5761 caggcctatg cccattttaa caaatggcgg atggatatga ttgtcgggcg tagcccggtg
     5821 ttgtgggggc agggtgaaca tggcggtcta ttgttcacgg ataacgcgag acctctggat
     5881 gtcatccaac tcacaaatgc tcaacccgta aggtttccct ggctgtttgg tcgtttgggt
     5941 gttttcaagt tcagttttgt ctttgggacg ctgggtcccg aacagttttt tccgcacact
     6001 ctgttttccg gattgagttt gggatacaaa ccagcttcgt ttttggagtt taatctctct
     6061 catgtgttgc aatttggtgg cgagggcgct ccagacctgg ggtttacgac cggtttgagg
     6121 gagttttttg ggtttattcc cggaatcagt cagaccgctg tcaggggggg caacaagatc
     6181 accgggttca accttcgttt ttttgccgac agactgatgg gccttcaggg gtatctggaa
     6241 tatgccatgg acgattccaa tctgtcgggt ggatcggccg cgctcaagaa acattttacg
     6301 gctaatagtt catatctggt gggcttattc tttacgcggc cttttgggtt gaccgtagat
     6361 tcattgagag tcgaaatgac ccgttttggg cagatcgcgt atcgtcattc tttgtatgac
     6421 ggagggtgga cgatcaatca acacatcatc ggccacccgt cggggcccga tagctacagt
     6481 gctcgcgtgg cctgggaaca tgattggtcc acgtttcggg accgatccaa ggtttttttt
     6541 caatggacta gccgtgacag cgatacttat caagtgagtg ccgatggtta taccgagacg
     6601 gtgctcaacg agggacccag tgaacagcgt tacgccgcta tggcagagca tgcctttgat
     6661 tggatgcgcc tgcaatggaa agtgggagcc ggattcgaaa tcattcaaaa ttacagatat
     6721 gttgagggaa atcgttcagg gactttactt ttaagctctc aaattaacaa gactttttaa
     6781 aatgttattg ttttttttga ggttagcttg ctatgaacgc gaggttgatt ttgttcataa
     6841 caatgagtgg ggggtaatca tatgtttgca tcaaaaagga ttgtttcgat tgttgggctt
     6901 gggtacgttg gacttccact ggcagtagct tttggcaagc tgggagaaac cattggtttt
     6961 gatgtgaacg aaaaacgggt gctcgagctc aaaaacggtc acgataaaac tctggaaaca
     7021 tccgatgtcg aactcaaggg gacgaatatt ttctacacca gtaaccccga ggatctcaaa
     7081 aaagcgaatt ttcatatcgt ggccgtgccc acacccatca actctgccaa acagccggat
     7141 ttggtccctt tgtacatggc ctccgatgct gttgggagtg ttctcaaaaa gggtgatgtt
     7201 gttgtttatg aatccacagt ttatccgggg gtgacagagg aagagtgtgt gcccattttg
     7261 gagcatgtgt cgggactgaa ggcaggcgtg gattttaagg tggggtatag tccagagcga
     7321 gtgaatcccg gtgacaaaga gaggacgctc gaaaaaataa ccaaaattgt ttctggccaa
     7381 gacgacgaga cactaaaaat tgtggcccaa acgtacggtc aaatcatcaa ggcgggtgtg
     7441 caccttgttt cgagcatcaa ggtggctgag gccgccaagg tgattgaaaa cactcagcgc
     7501 gatttgaaca tcgccctcat gaatgaactc gctatcattt ttgaacacat gggcatcgac
     7561 accaacgagg tgatcgacgc ggccggcacc aagtggaatt ttttaaaatt cagacctggt
     7621 ttggtgggtg ggcactgcat tggtgtggac ccttactacc tcacgcataa ggctgaaaaa
     7681 ctgggctatc atccgcaggt gatattggcg ggacgtcgaa tcaacgacgg tatgggtaaa
     7741 ttcattgccg gacagacggt caagcagatg attcgtgcag ggcataatat tctcaacaac
     7801 acagtgacca tgttggggat tacgttcaag gaagattgtc ccgatattcg caattccaag
     7861 gtggtcgaca ttatcaacga actcaaggaa tttggactga acgtacagat ccacgatccc
     7921 gtggccgacg tggaagatgt taaacgcgaa tatggtgtgg agttgactcc ctataacgag
     7981 ctcaaaccgg cggcggcggt ggttgtggcg gtcgcgcatg aacaatacaa gaaactcctg
     8041 ccagctgatt tgcaaaaatt gactgttccc caggccgtgt tcatcgacgt gaaaaacgtg
     8101 tacaacccaa aggcattgtt ggagatgggg tttaaggtgt ggaggctgta aagaacgacg
     8161 gacgagaaca cgagggacga aaggacgaga cgtaccgtca tggcgagcct gagggtagcg
     8221 aag
//
LOCUS       DATDUN010000539         1153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_28718, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000539 DATDUN010000000
VERSION     DATDUN010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28718"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VJC18_07580"
     CDS             <1..1057
                     /locus_tag="VJC18_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HLD45280.1"
                     /translation="IQYRGRPNVNIEARLGSESYGLFANVELKEFKRVLSNVINNAVE
                     AFDNEGEVVVAMRAVEPQSRGANDSEFTEAPTPHGSMAQEIQITITDTGKGIPADILP
                     QLMNQGATFGKEKGQGLGLYHARETIRAWGGDIAIESKVGKGTTVTLTLPKEKEPEWF
                     VGELVVRGSWVVNREGNNNKLQTGEQSTVTPIASQNIVLTERETTTKNLPYAIHTLWG
                     TSDPRSIPSGAQAIHASPKSILILNDDAGLLLSMKIILKREGHTVIGAANVQEAIKLM
                     TEHKIDFVLSDINLGENQPDGFEFIKHVRTKDTALPFYFMSGYQASELRSKALSEGAT
                     GYLQTPVSKDELMVLVK"
BASE COUNT          313 a          278 c          323 g          239 t
ORIGIN      
        1 catacagtat cgcggtcgcc cgaacgtcaa cattgaggcc agactgggga gcgagtcata
       61 cggcctcttt gccaatgtgg agctgaaaga attcaagcgc gttctctcaa acgtcatcaa
      121 caacgccgta gaagcctttg ataatgaggg ggaagtggtg gttgctatga gagccgtaga
      181 gccacagagc cgtggagcca atgattcaga atttaccgag gctccaacgc cccatggttc
      241 catggctcaa gaaattcaaa tcaccatcac cgacaccggc aaagggattc cagcagacat
      301 tttgccccaa ctcatgaatc agggggcgac gtttggcaaa gagaagggtc agggactggg
      361 cttgtatcac gcgcgcgaaa cgattcgagc gtggggtggc gatattgcca ttgaatcaaa
      421 agtgggcaaa gggacaactg ttactttgac tttgcccaaa gaaaaagaac ctgagtggtt
      481 tgtgggggaa ctcgtggttc gtggatcgtg ggttgtgaat cgagagggaa ataacaacaa
      541 acttcaaacc ggtgaacaat caactgtgac acctattgcc agtcaaaaca ttgttttaac
      601 agaaagagaa acaacaacaa agaacttgcc ctatgcgatc cataccctct ggggcacaag
      661 cgatccacga tccataccct ctggggcaca agcgatccac gcttccccca aatccatcct
      721 catcctcaac gatgacgctg gtttgctgtt atcgatgaag atcattttaa aaagggaagg
      781 tcacacggtg atcggcgcgg ccaatgtgca ggaggccatc aagctcatga cagagcacaa
      841 gattgatttc gtgttgtccg atattaatct gggcgagaac cagccggatg gttttgagtt
      901 catcaagcac gtgcgcacca aagataccgc cctgcctttt tatttcatgt cgggctatca
      961 ggccagcgaa ctccgctcca aagccctctc cgagggcgcc acaggttacc tgcaaacccc
     1021 cgtctccaaa gatgagctga tggtgttggt gaagtaggcg gaagggtaat gagtaatggg
     1081 taatgggagg gggagaaggg tcgcgggaac gcggggtggg tagacgtgtt caccccctca
     1141 gggggcaggc gtg
//
LOCUS       DATDUN010000540         1967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_194002, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000540 DATDUN010000000
VERSION     DATDUN010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194002"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VJC18_07585"
     CDS             <1..640
                     /locus_tag="VJC18_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45281.1"
                     /translation="TGEFEDGSLGEIFLDMHKEGAAFRSLMNCFAIGISIGLQYGVPL
                     EEYVEAFTFTRFEPNGMVKGHDNIKMATSVIDCIFRDLAMNYLGRYDLVHITPEDMRN
                     TSVKNVGELDETIDYDAQGKEKANVMPKELPISTAGGMQEVTSQPVLHATAPVTTVST
                     GNKKIPARSSRHVEAKLKGYEGDPCPDCGQFTLVRNGSCLKCNSCGATTGCS"
     gene            845..1306
                     /locus_tag="VJC18_07590"
     CDS             845..1306
                     /locus_tag="VJC18_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HLD45282.1"
                     /translation="MKNLDFIFIGSPKNKSCCALELEYHKRIAHYTATQLITLKDSGD
                     KNILLKNKKETQTIVSKLTPSDFLVVCDERGQAPGSVAFAKNIAQWRNSHQRVVFVVG
                     GAYGLGEEVLHKANVKLKLSEFTLPHELARIVLLEQTYRAFTILGGEKYHH"
     gene            complement(1602..>1967)
                     /locus_tag="VJC18_07595"
     CDS             complement(1602..>1967)
                     /locus_tag="VJC18_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLD45283.1"
                     /translation="IAPYKKIAEKKILTVLGTDGIPSVPNLSLWDEMRAFVTHHMDSL
                     PLTGFGVMSMVTTHAAQALGLAEEIGSIEKGKKADLILVDLTDISTEGDLLMNMIRGV
                     SNYHIKSVMVNGKNVKSMN"
BASE COUNT          524 a          495 c          480 g          468 t
ORIGIN      
        1 taccggcgag tttgaggatg ggtcactggg cgaaatcttt ctcgacatgc acaaggaagg
       61 tgccgcgttc cgttccctga tgaactgttt tgccatcggc atatcgattg gcttgcaata
      121 tggcgtcccg ctcgaagaat atgtcgaggc cttcacgttc acacgtttcg aacccaacgg
      181 catggtcaaa ggacacgaca acatcaaaat ggccacatcc gtcatcgact gcatcttcag
      241 ggatttggcc atgaactatc tgggtcgtta cgacctggtg cacatcactc ccgaggacat
      301 gcgcaatact tcggtcaaaa acgtgggtga gcttgacgaa accatcgatt acgatgccca
      361 gggcaaagaa aaagccaatg tcatgcctaa agagcttccc atcagcacgg cgggggggat
      421 gcaagaggtc acctcgcaac ctgttttgca cgcgaccgca cctgtcacga ctgtttctac
      481 aggcaacaaa aaaataccag cacgttcaag ccgacacgta gaggccaaac tcaaagggta
      541 tgaaggtgat ccttgtcccg attgcgggca gttcaccctg gtgcgcaacg gttcttgctt
      601 aaaatgcaat tcgtgcggcg ccacgacggg gtgttcatga ctcgtggatc gtgaatcgta
      661 aatcgatttg tcatccctgc gacccttcga caggcgctca gggtggtgag cgaagtcgaa
      721 ccaaaacagg gatctagaat tatcgacaac atggatcccg gcgtacgctg ggatggcgtt
      781 cgccctttca ttcctcgtat gattcatacc caaaagggca caagcgatcc acgctcaacg
      841 agttatgaaa aatctggact ttatttttat tggctcccca aaaaacaaat cctgttgtgc
      901 cttggagctt gagtatcaca aacgcattgc ccattacaca gccacacaac tcatcacgct
      961 caaggacagt ggcgacaaaa acattctcct aaaaaacaaa aaagaaacac aaaccatcgt
     1021 gtccaaacta accccttcag atttcttggt ggtctgtgat gagagaggcc aagcgcctgg
     1081 ctcggttgct tttgcgaaaa acatcgcaca gtggcgaaac agtcatcagc gcgttgtctt
     1141 tgtggtcggc ggcgcttatg gcttggggga agaggttttg cacaaggcaa acgtcaagct
     1201 caaactatcc gaattcaccc tgccacacga actggcccgc attgttttgt tggaacagac
     1261 ctaccgcgca ttcaccattc tgggtgggga aaaatatcat cattgatgac attcacttag
     1321 gaaggcaatg catttttaac atttctgctt aaggggacct ctaaaaacta tgttcgttac
     1381 gaaaaaccat gattcgtagc cgagacaagg aagatgagta aaaagcccgg aaacgtataa
     1441 ggcacgttac gttgaggact ttttacgaaa tctgacaaag tcgcaggccg aagcatggtt
     1501 ttgcagtaga agatagtttt tagaggtccc cttaagattg cctcttatta agagcccgga
     1561 ccatgcgggc gacagaagtc atttgtttga aaatgctgga atcaattcat cgatttgaca
     1621 ttcttgccgt tcaccatgac gcttttgatg tgatagttgc tcacgccacg aatcatgttc
     1681 atgagcaaat caccttcggt ggaaatatcg gtgaggtcca ccagaatcag atcggccttt
     1741 ttgccctttt cgatggaacc gatttcttcg gccaacccca acgcctgcgc cgcgtgcgta
     1801 gtcaccatcg acataacccc aaagcccgtg agcggcaggg aatccatgtg atgtgtcaca
     1861 aaggcacgca tctcgtccca cagtgataaa ttgggtaccg aggggatgcc gtcggtgccc
     1921 aatacggtca ggattttctt ttcggcgatt tttttgtacg gcgcgat
//
LOCUS       DATDUN010000541         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_277751, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000541 DATDUN010000000
VERSION     DATDUN010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277751"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1676
                     /locus_tag="VJC18_07600"
     CDS             333..1676
                     /locus_tag="VJC18_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012220584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45284.1"
                     /translation="MLNDHQINNLFWKSEENFQNNDPHLSQLKDLPMVHPMDWWKKID
                     FNSSGICILTGGRQIGKSTSLKLMIRHVLSKKLFTPSQIFYLTCDMIFDPQHLMRTIK
                     SFLDLSLDENKPFLLLLDEVTFVPSWDRVVKALADEGIFRRGLCVLTGSDTVILKQAS
                     SSFPGRRGKSDIVDFHVRPLSFAEHVRLVAPDTFSAPEKNLDALFVLFDQYLLCGGFL
                     RAMNDLSQQGKVTEATFQTFEQWLRGDFLKRGKSEEYLLSVLKTLSEVGVTQNSYSGL
                     TQRAGNMSKETFIDYCWLLQRMDVLFTIEAFDQNTLRGFPKKAMKFHFWDPFIARVIR
                     RWLVRERMVNAEINEGNMVESSVASNCYHRFPTYYIKAEGEVDAVIVEESKFYPIEVK
                     WTEQLRSIDLKQIKKYKNSFIVAKSHEEGFIENVKVIPLPLFLLTFGFDPKNQKG"
     gene            complement(1731..>2588)
                     /locus_tag="VJC18_07605"
     CDS             complement(1731..>2588)
                     /locus_tag="VJC18_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45285.1"
                     /translation="DAQGAQALGVALGQATQASLVIEKDKSTYGQDIRGGNSYETAVP
                     LESNTEYRLDHHQLKEYYDYFYVDLQASQEITVELKTLEKGVNLKDGQAIENNNPYAA
                     IALHDSERNKLNSKNIIGDANRTEVITYCAARTGRYYLLLGSVYAAQHKDHVTFKLKT
                     TFKGDLGSQSDAGADMKTALRLAPGRYDTNYLGGSDEVDYFVFDVQAGDKYYVGIIPG
                     DEFSNCSFNVAIFDEFKQKKLEQSSRFGEGLKTPPITFVEGGTHYLSVGLRGTLSKTG
                     TYTLVLEKI"
BASE COUNT          642 a          609 c          613 g          724 t
ORIGIN      
        1 ggcatcgaca ggatctgccg ggtatttggc aaaaacagcc gtgcctgtct gaaggtgaca
       61 gaatattacg atacagaagt tcccccgcct cctttgctaa gcacggtgcc cccgtcgccc
      121 tcgccaatta atgagctcta aacctgttcc agcaccaacg tgtaggtccc agtatttgac
      181 agtagaaaca tgaagacgta gttttttatg tagcagataa ctctatgtat acattatata
      241 aacttggttg ttttgtgttt gtctaatgta tattagacag atttgtctaa tagagaatgg
      301 acaaatcctt ccttttatga aatgatgtgg tcatgctcaa cgaccatcaa atcaacaatc
      361 tgttctggaa atcagaggaa aattttcaaa acaacgatcc ccatctttct caacttaaag
      421 atctccccat ggttcatcct atggactggt ggaaaaaaat tgatttcaac agctctggta
      481 tttgcattct gacaggtggg cgtcaaattg gaaaatctac atcgctcaaa ctcatgattc
      541 ggcatgtcct ctcaaaaaaa ctttttactc catcccaaat attttacctg acctgtgata
      601 tgatctttga tcctcagcat ttgatgcgaa ccatcaaaag tttcttggac ctgtcgctgg
      661 atgaaaacaa gccctttctt cttttgttgg atgaagtcac gtttgtccct tcttgggatc
      721 gcgtggttaa ggcacttgcc gacgagggaa ttttcagacg tggcctttgc gtcctcacag
      781 gctcagacac ggtcattctt aagcaagcat cctccagttt tcccgggcga cgaggcaaga
      841 gtgacatcgt cgattttcat gtacgccccc tctcctttgc tgaacatgtt cggcttgttg
      901 ctccagacac attttcagct ccagaaaaaa atctggatgc cttgtttgtt ctctttgacc
      961 agtatctcct gtgtggcggc ttcttaaggg ccatgaatga tctctcacag caaggaaagg
     1021 tcacagaggc cacgttccaa acgtttgaac aatggttgcg cggtgacttt ctgaagcgtg
     1081 gaaaaagcga agagtatctt ctgagtgttt tgaagaccct gtctgaggtt ggagttaccc
     1141 agaattcgta ttctggcctg acgcaacgtg cgggaaacat gagcaaagaa accttcattg
     1201 attattgttg gttgttgcaa cgcatggatg ttttattcac gattgaggcc tttgatcaaa
     1261 acaccttacg aggttttcca aaaaaggcca tgaagtttca cttttgggat ccgttcatcg
     1321 cacgtgtgat tcgtcgttgg cttgtgcgag aacggatggt gaacgcagag attaacgagg
     1381 gcaacatggt tgaatcaagc gtagcctcaa attgttatca ccgatttccg acgtactata
     1441 tcaaagctga aggtgaagta gatgctgtga tcgtggaaga gagcaagttc tatcccattg
     1501 aggtgaaatg gacagaacag cttcggtcaa tcgacctcaa gcagatcaag aaatacaaga
     1561 attctttcat tgttgccaaa agtcatgagg agggcttcat agaaaatgtg aaagttattc
     1621 ctctgccgct cttccttctc accttcggtt ttgaccctaa aaaccaaaaa ggttgaggcc
     1681 ttcgagggga taacctgccg cttcgtcgca aaatagagtt tctgttgccc ttatatcttt
     1741 tccagcacca gcgtgtaggt cccggttttt gacagagttc ctcttaatcc caccgacagg
     1801 taatgcgtgc ccccctccac aaaggtgatg ggtggagttt taaggccctc gccgaaacgg
     1861 gagctttgct cgagcttttt ctgcttgaat tcatcgaaga tcgcgacgtt gaaagaacag
     1921 ttcgaaaatt catcgccggg gatgatcccc acgtaatatt tgtcgccagc ctgcacatca
     1981 aaaacaaaat aatcgacctc gtcactgccg ccaagatagt tggtatcata tcgcccggga
     2041 gccagacgca gagcggtttt catgtcggcg ccggcatcac tttgagagcc gagatcaccc
     2101 ttaaacgtgg tcttgagctt aaacgtaacg tgatccttgt gttgcgccgc gtagacagac
     2161 ccgaggagca ggtaataccg gccggtgcgc gccgcgcagt acgtgatgac ctctgtgcgg
     2221 ttggcatcgc caatgatgtt cttgctgttg agtttgttgc gctcggaatc gtgcagagcg
     2281 atcgccgcgt aggggttatt gttttctatg gcctgcccgt ctttgaggtt cacacccttt
     2341 tcgagtgttt tgagttcgac agtgatctcc tgtgaggcct gcagatcgac ataaaaataa
     2401 tcgtagtatt cctttagttg atgatggtcc agacggtatt ccgtattgct ctccaagggt
     2461 acggctgtct cataagaatt gccgccgcgg atatcctggc cgtaggttga tttatctttt
     2521 tcgatcacga gcgaggcctg cgtggcctga ccaagagcga cacccagcgc ctgcgcccct
     2581 tgggcatc
//
LOCUS       DATDUN010000542         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_109353, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000542 DATDUN010000000
VERSION     DATDUN010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109353"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VJC18_07610"
     CDS             complement(<1..403)
                     /locus_tag="VJC18_07610"
                     /inference="COORDINATES: protein motif:HMM:NF039198.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HLD45286.1"
                     /translation="MDFTTMLHVLFLGLIVFGARVIDVSLGTLRTINIIYGRAKIAFI
                     LGFLETFVWVYVIATILKYIAQYPVLGVFYALGFATGNVVGVAIEKKLAFGYMLLRVI
                     AMESADTMASSLRDEGYRVTTFAGEGRDGPVT"
     gene            complement(410..1993)
                     /locus_tag="VJC18_07615"
     CDS             complement(410..1993)
                     /locus_tag="VJC18_07615"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLD45287.1"
                     /translation="MKTSILICVLLLLACPVVFSQEGNTDNSEPSENIRTILEQSRGE
                     SQYAIKREKIALLATVESFYTQRSFEPAWFKSNKITHQLEEMMTVLKDSGFEGLDPQD
                     YHLSFLETAITKLKDKNNFNEKRLLTLAQADVLLTDAYFQYASNMSTGRLERLDLDPQ
                     WHIEGRQKLEYIVQLNTDLTNDTVAASLIALQPVHKEYVQLKNKLKEMLVAQEKAYGK
                     KAQELQENIAKVKINLERWRWLPQQLEDRYILVNAADFTLDVIEKDVSQLHMKVIVGK
                     AQRETPAFSDVMTYVVFNPSWHAPRSIAIRDKLPLLKQDAAGLAKQGFQVFDEEVDDG
                     VPISPDTIDWSTVTEENFKYRIQQLPGPTNPLGRIKFMFPNQFDIYLHDTPDRFLFEK
                     DQRMYSSGCIRIERPLDLAEYLFKVRDEALDEGTRDEGRKDERTNIEKSKEDKTIWTL
                     EKITAAIEAGKEQTVVLPEPIRVYILYITAWVDDNGELHFRDDIYGRDQKVDQKTLIS
                     TPKKECMYGMKCWFKNLIN"
     gene            complement(2097..>2401)
                     /locus_tag="VJC18_07620"
     CDS             complement(2097..>2401)
                     /locus_tag="VJC18_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45288.1"
                     /translation="GRTEGLTKLIIDAKTEKILGVGLVGKGVGDMISEGALAVETGVT
                     ARELADTIHPHPTLSESLMEAAEVYYGVCSHLYPSTQSRHGTLVPKKNKTKDTLSE"
BASE COUNT          579 a          667 c          533 g          622 t
ORIGIN      
        1 tggtcacggg accatcgcgc ccctcccctg caaaagtagt cacgcgataa ccttcgtccc
       61 tcagactgct ggccatggta tcggcagact ccatcgcgat caccctgagc aacatatagc
      121 caaaggccaa ttttttttcg atggccactc ccaccacgtt gcctgtggca aaacccaggg
      181 catagaacac acccaaaaca gggtactgtg cgatgtactt taaaatggtg gcaatgacat
      241 acacccagac aaaggtctcc aaaaacccca aaatgaaagc gattttggcc ctcccataaa
      301 tgatgttgat cgtgcgcaaa gtccccagac tcacatcaat aacgcgcgca ccaaaaacaa
      361 tcaggcccag aaataaaaca tgcaacattg tggtaaaatc catagatcct caattaatca
      421 aatttttgaa ccaacacttc atcccgtaca tgcattcctt cttgggtgtg ctgatgagcg
      481 ttttttggtc gaccttctgg tcgcggccgt agatgtcatc cctgaagtgt aactcaccgt
      541 tgtcatccac ccaagccgtg atgtacaata tgtaaacgcg gatcggctcg ggcaaaacca
      601 ccgtctgttc cttgccggcc tcaatggccg ccgtgatttt ctccagcgtc cagattgttt
      661 tatcttcctt tgatttttct atgttcgtcc tctcgtcctt tcgtccctcg tccctcgtcc
      721 cttcgtccag cgcctcatcc ctgaccttaa acaaatactc cgccaaatcc agcgggcgct
      781 caatgcggat acagcccgaa ctgtacatac gctggtcttt ttcaaacaga aacctatcgg
      841 gcgtgtcgtg cagataaata tcgaactggt tgggaaacat gaatttgatt ctgcccagag
      901 ggttggtggg cccgggcaat tgctgaatcc ggtatttaaa attctcttct gtcacggtcg
      961 accaatcaat cgtatcgggc gaaatcggca caccatcgtc cacctcttcg tcaaaaacct
     1021 gaaagccctg cttggccagg ccagccgcgt cctgcttgag caagggcaat ttgtccctga
     1081 tggcgattga tcgcggggca tgccaactgg gattaaacac cacgtacgtc atgacatcgg
     1141 aaaaagccgg cgtctcacgt tgcgccttgc ccacaataac cttcatgtgc aactgagaga
     1201 catccttctc gatcacatcg agcgtgaaat cagccgcgtt cacaagaatg tatcgatcct
     1261 ccagttgttg cggcaaccag cgccaccgtt ccaggttgat cttgaccttg gcgatgttct
     1321 cctggagctc ctgcgctttt ttaccatacg ccttttcctg agcgaccagc atttccttga
     1381 gtttgttctt gagttgcaca tattctttat ggacaggctg caaagcaatg agggaagcgg
     1441 ccacggtgtc gtttgtcaaa tccgtgttaa gctgaacaat gtattctaac ttctgacgac
     1501 cctcaatgtg ccactgggga tcaagatcaa gtctttccag acgacccgta gacatgttgg
     1561 aggcgtactg aaaataggcg tccgtcaaca acacatccgc ctgagccaaa gtcaataaac
     1621 gtttttcgtt aaaattattt ttgtctttga gtttggtgat ggctgtttcc aaaaaactca
     1681 agtggtagtc ctgcgggtcc aagccctcaa atccggaatc cttcagcacc gtcatcatct
     1741 cttcgagctg atgcgtgatt ttgttgctct tgaaccaggc cggttcaaat gagcgctgcg
     1801 tgtaaaagga ttcaacagta gcgagcaagg caattttttc gcgcttgatg gcatactggg
     1861 attcccctct gctctgctcc agaatggtgc ggatattctc ggacggctcc gagttgtccg
     1921 tgttgccttc ttgagaaaag acgacgggac acgccaacaa caacagaaca cagattaata
     1981 tagacgtttt catattactt ctttggttta tcagcaactc gctagcaagg acttcgaccg
     2041 agttcagtcg agggcctacc gagtttgatc ctgagcgaag aacccttcga ctcgtttcac
     2101 tcgctcaggg tatccttcgt cttgtttttt ttcggcacca aggtgccgtg tcttgactga
     2161 gtcgaaggat ataaatgact gcaaacccca taatacactt cggcggcctc catgagggat
     2221 tccgacaacg tgggatgggg atgaatggtg tcagccaact ctctggcggt caccccagtt
     2281 tcgacagcca aggctccttc actgatcata tcaccaacac ccttccccac cagacccact
     2341 cccagaattt tttcggtctt ggcatcgatg atcagtttgg tcaggccctc tgttctgccc
     2401 a
//
LOCUS       DATDUN010000543         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_277818, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000543 DATDUN010000000
VERSION     DATDUN010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277818"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2070
                     /locus_tag="VJC18_07625"
     CDS             <1..>2070
                     /locus_tag="VJC18_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45289.1"
                     /translation="EEEDPQDSRQTDLADSMTSAGITFMVADPVDDILYMANDDGYFF
                     QYNLNTLQLDEVLLSYGEIEEDEVTEEGEETTASNYYIPTAMVYAESSDGDKILITTT
                     TGEVIVITPGGTGYTVYTLTSSATTFETTSPNLSDISLTPDGNYAYVVDSDNDLVWVF
                     HVVSEQFIDQQSSGTSLDPIELEDVDDNNAFTHILIYSDAGGDVAYLAGANGLTLIDA
                     ETPGTATATKIIDPDTTTTGENPIALSGTPGPLITTSADTGYLFSINGDASISVLTDN
                     PWITLSSISTDTVTTETASTFSLTFQSDTAGSYTIKANSDLTADSGTELIASTAFAEV
                     DTDITTADIDVNSFARTSFVEGENKIFVFVTDVNSLLGHSAIILNVDRPPEPVTITGV
                     SFGNRKAYVQFEASPDADIDHYVLYAEPAANQATPACPGALTFVSDNAITTNVSTSVC
                     TDDTCEGTANNLTNDTAYCVAVSVVDESDQASALSAYTQSVTPEQTVGPAGYFGETSC
                     SLNSLLHKTNTSPVSFVVLLGGLGLLLMTRFCSKEKCHPSVPQTGSVLRLKCWDPDLV
                     KSWIPAFAGMTKLILILSLMSFSHSALAQERSPQHWTLEPKLSFWIPTDTAVRDFMGL
                     CCHIGGEVEFGYLIKNQFNITATTGFSYDGGQSVGLRSGGASGDSYNLFFFPFRFDFL
                     YRFDYKP"
BASE COUNT          501 a          609 c          477 g          483 t
ORIGIN      
        1 atgaggaaga agatcctcag gattcgcgcc aaacagattt ggccgacagc atgaccagcg
       61 ctggcatcac atttatggtc gccgaccctg tggatgacat cctttacatg gccaatgacg
      121 atggttattt ttttcaatac aacctcaaca ctttgcaact tgacgaggtc ctgctcagct
      181 acggcgagat tgaagaagat gaggtcacag aagaaggaga ggaaacgaca gcatcgaatt
      241 attacattcc cacggcaatg gtgtatgccg agtccagcga tggcgataaa attttgatca
      301 caacaaccac aggagaggtc atcgtcatca ctcccggcgg cacagggtac acagtctaca
      361 cgctcacctc atcagccacc accttcgaaa ccacctcgcc caacttgagc gatatctccc
      421 tcacacccga tggcaactac gcctatgtgg tcgattctga caacgatttg gtctgggtgt
      481 ttcatgtggt ttccgaacag tttatcgatc aacaaagctc cggcaccagc ctcgatccca
      541 ttgagctgga agacgttgac gacaacaacg ccttcacaca tatcctcatt tatagcgatg
      601 ccggtggcga tgtggcctat ctggccggtg ccaacggact cacactcatc gatgcggaaa
      661 cgccgggaac tgcgacagca acaaaaatca tcgaccccga caccacgacc accggtgaga
      721 atcccatcgc tctgtcgggc acacccggtc cgctcatcac aacatcggct gataccggct
      781 acctattttc catcaacggt gacgcctcga tctcagtcct cacagacaac ccgtggatca
      841 ccctatcgtc gatcagcacc gacaccgtca ccacggaaac agcctccact ttttcgctca
      901 cgtttcaatc cgacacggcc ggctcctaca caatcaaagc caacagcgac ctcaccgccg
      961 attccggcac ggaactcatt gccagcacgg ctttcgcgga agtggatacc gacatcacaa
     1021 cagccgacat cgacgtcaac agctttgccc gcacctcttt tgtggaaggg gaaaataaaa
     1081 tatttgtttt tgtcactgac gtgaatagcc ttctgggcca cagcgctatt atcttgaacg
     1141 tcgatcgccc cccagaaccg gtgaccatca cgggggtcag ttttggcaat cgaaaggcct
     1201 acgtgcaatt tgaggcctcg ccggatgccg atatcgatca ctatgtgctc tatgccgaac
     1261 cagcggcgaa tcaagctacg ccagcatgtc ctggcgctct gacctttgtt tccgacaacg
     1321 ctatcaccac caacgtgagc acgtctgttt gcactgacga cacctgtgag gggacggcca
     1381 acaacctcac caatgatacc gcctactgtg tggccgtttc ggtcgtcgac gaaagtgatc
     1441 aggccagcgc tctttcggcc tacacccaat cggtgacccc tgagcagacc gtgggacccg
     1501 ccgggtattt tggagaaacc agttgttcgt tgaacagtct tttgcacaaa acaaacacat
     1561 ctcctgtctc ctttgttgtt ctgctaggcg gtttggggct gttgttgatg accaggtttt
     1621 gttccaagga aaaatgtcat cccagcgtcc cgcaaacggg atcggtccta cggctgaaat
     1681 gctgggatcc agatttagta aaatcatgga ttcctgcctt cgcaggaatg acaaaactta
     1741 tattgattct ttccctgatg tctttttccc acagcgcact ggcacaggag cgctctcccc
     1801 aacactggac actggaaccc aaactctctt tctggattcc caccgacact gccgtgcgtg
     1861 attttatggg cctgtgttgt cacatcggcg gcgaggttga gtttggttac ctcatcaaaa
     1921 accaattcaa catcaccgcc acaacaggat tctcttatga tgggggacag tctgtgggac
     1981 tgcgctctgg tggtgcctcg ggagattcct acaacctgtt tttctttccc tttcgtttcg
     2041 attttctgta ccgatttgac tacaaaccgg
//
LOCUS       DATDUN010000544         1648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_398584, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000544 DATDUN010000000
VERSION     DATDUN010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398584"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VJC18_07630"
     CDS             complement(<1..682)
                     /locus_tag="VJC18_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HLD45290.1"
                     /translation="MLLTQLLNKRVLFVSGKGGVGKTTVSVALALLAARQKKKTLIVE
                     MNSSGRVAPLFGAESAEHDEIALAPYVWGINLSPARCFEEYVLRQVRFKKIYDVFFNN
                     YYVNNFIKAVPGLNEVLMLGKIQNLESQRKNRFAEKKEYDLIVVDAPATGHGLSVFEV
                     PQVVLSAVGMGPLHHHAKHIQELLADEQKTFFCLVTLAEEMSVVESKDYVSALRERTE
                     LHFGFVFIN"
     gene            complement(694..1251)
                     /locus_tag="VJC18_07635"
     CDS             complement(694..1251)
                     /locus_tag="VJC18_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HLD45291.1"
                     /translation="MKDALKKPIIIKRYQNRKLYDTQNSTYVTLDEIGAMIRRGEDVR
                     VIDNKNKEDLTGVTLTQIIFEEEKKNKSLLPLQSLKNIIREGGGALKGLLHKTTDTVQ
                     NKISTAKEGAENFYDKIEGAFGSTDDNLLKEVLQRTQDISKNIEGKIKTTVDSFTHVA
                     SLQNEVRKLRQRVLYLEKKLRHYEK"
     gene            complement(1282..>1648)
                     /locus_tag="VJC18_07640"
     CDS             complement(1282..>1648)
                     /locus_tag="VJC18_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45292.1"
                     /translation="SESIELTNRVAEETPILQKWQGLEPLLSTIDSVIRFSVRQLSLG
                     DPLAEQDNREDKMRAEIVTSIIQALDEEIESVGGRADSNRQLKIDALMAVRNVLVKQS
                     RPKSAEADNVILLNKAVNE"
BASE COUNT          371 a          434 c          375 g          468 t
ORIGIN      
        1 cgttgatgaa aacaaatcca aaatgaagct ctgttctctc cctgagggcc gagacgtagt
       61 ccttggactc cactaccgac atctcctcag ccagggtgac cagacaaaaa aatgtttttt
      121 gctcgtcagc caatagttcc tgaatgtgtt tggcgtggtg atgcagaggg cccatgccca
      181 cggcagacaa cacaacctgt ggcacttcga aaacagacag gccatggccc gttgccgggg
      241 catcgaccac gatcaaatcg tactctttct tttccgcaaa acgatttttc cgctggcttt
      301 ccagattctg gattttgccc aacatcaaga cctcgttcaa ccccggcacg gccttgatga
      361 aattgtttac atagtagttg ttgaagaaaa catcgtagat ttttttgaac ctgacctgtc
      421 gcagaacata ctcttcaaaa cagcgcgccg ggctgagatt gatcccccat acataagggg
      481 ccaaagcgat ctcatcgtgc tctgcgctct cggccccaaa gagcggggcc accctgccag
      541 atgaattcat ttcgacgatg agggttttct ttttttgcct ggccgccaac agggccaacg
      601 ccaccgaaac cgtggttttc cccaccccgc ccttgcccga cacgaacaga acgcgcttat
      661 tgagaagttg tgtgagcagc atatgacgat tcactatttc tcataatgtc gtaacttctt
      721 ttcgagataa agcacgcgct gtctgagttt gcgaacctcg ttctggaggc tcgccacgtg
      781 cgtgaaggaa tcgacggtgg tcttgatttt gccttcgatg tttttggaga tgtcttgggt
      841 gcgctgaaga acctctttca acaaattatc atccgtggac ccaaaggccc cctcgatctt
      901 gtcgtaaaag ttttcggcgc cttctttggc ggtggaaatc ttgttttgca cggtatcggt
      961 ggttttgtga agcagtccct tgagcgcccc gcccccctca cgaatgatgt ttttgagtga
     1021 ttgcaggggc agtaggcttt tgttcttttt ttcttcctca aaaatgatct gagtcagcgt
     1081 gacgcccgtc agatcttcct tgtttttatt gtcgatcacc ctcacgtcct caccgcgtcg
     1141 gatcatggcg cctatctcat cgagggtgac ataggttgaa ttctgcgtat catacaattt
     1201 gcgattttga tatcgcttga tgatgatcgg tttttttaag gcgtccttca caatccctcc
     1261 tgtgttcatg ctcaaaaaaa atcattcatt aacagctttg ttaagcaaaa tgacattgtc
     1321 tgcttcagcg gacttgggtc ggctttgctt caccagcacg ttcctcacag ccattaaagc
     1381 atcgatcttg agttgtcgat tggaatcagc tctacccccg acagattcaa tctcctcatc
     1441 caatgcctga atgatggacg tgacaatttc ggccctcatt ttgtcttccc tgttgtcctg
     1501 ctctgcaagc gggtccccca gactgagttg tctgacagaa aatcgaatca cagagtcaat
     1561 cgtgctgagc aaaggttcta aaccctgcca tttctgtaat atcggcgttt cttcggcgac
     1621 ccgattagtg agttcaatgg attcggac
//
LOCUS       DATDUN010000545         1053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_484385, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000545 DATDUN010000000
VERSION     DATDUN010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484385"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1053
                     /locus_tag="VJC18_07645"
     CDS             <1..>1053
                     /locus_tag="VJC18_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HLD45293.1"
                     /translation="NKLIETHKPDHMIVCYDRPEKGFRHELYPAYKANRSAPPEDLVP
                     QFEIINEFIRTYPIKSLAKPGFEADDLIATLVARYKTIPDMQIYIVTSDKDLMQLVDD
                     NVFIYDTMKEKIITRKEVVEKFGVPPEKVIDVQSLSGDPTDNIPGIVGVGPKTATKLV
                     LEYGSLEAVLDHASEIKGKLGEKIAQGRANALLSRQLVTLPTQVETQADWDDLVQPPA
                     DVSALNTFYQKLDFTKFISRQWQETPSVPQTQKAVFVGITSLDELKALVDNILQTKPQ
                     CLAFDTETDSLDAHNATLVGLSLCYQQDKAYYLPLAHREGPNLPFADVVRMISPLLVS
                     NDLAKAGQNSKFDLNVL"
BASE COUNT          291 a          275 c          254 g          233 t
ORIGIN      
        1 aacaaactga tcgaaaccca caaacccgat cacatgatcg tgtgctacga ccgtcccgaa
       61 aaaggatttc gtcacgaatt gtacccggcc tataaggcca acagaagcgc acctccggaa
      121 gatctggtgc cgcaatttga gatcatcaac gaattcatca ggacttatcc catcaaatcc
      181 ttggccaaac cgggttttga ggccgacgac ctgatcgcca ccttggtggc ccgttacaaa
      241 accattcctg acatgcaaat ctacatcgtg acctcagaca aggacctaat gcagttggtg
      301 gacgataatg tttttattta tgacacgatg aaagagaaga tcatcacccg taaggaggtg
      361 gtcgagaagt ttggtgtgcc tcctgaaaaa gtgatcgatg tgcagagcct gagcggagac
      421 cccaccgaca acatccccgg gattgtaggc gtggggccaa aaactgccac caaactcgtg
      481 ctggaatatg gctcattgga agctgtgctc gatcacgcca gtgagatcaa gggcaagctg
      541 ggtgagaaaa tcgcgcaggg cagggcgaac gccttgttat ctcgtcaact ggtcacactg
      601 cccacacaag tggagaccca ggcagactgg gacgatcttg tccaaccgcc cgcagatgtc
      661 tctgccttaa atacatttta tcaaaagctg gattttacaa aattcatcag cagacaatgg
      721 caagaaacac cgtccgtgcc acaaacacaa aaggctgttt ttgtcggcat cacaagtctc
      781 gatgaactca aagccttggt ggacaacatt ctgcagacca agccgcagtg cctggccttt
      841 gataccgaaa ccgattcatt ggatgctcac aacgcaaccc tggtggggct gagtctgtgt
      901 tatcaacagg acaaggccta ctaccttccc ctcgcacatc gcgaagggcc caatttgccg
      961 tttgctgatg tggttcggat gatctcacca cttttagtta gcaatgacct tgccaaggcg
     1021 gggcaaaatt cgaaattcga tttgaatgtt ttg
//
LOCUS       DATDUN010000546         1280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_194196, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000546 DATDUN010000000
VERSION     DATDUN010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194196"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VJC18_07650"
     CDS             complement(<1..801)
                     /locus_tag="VJC18_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HLD45294.1"
                     /translation="MKKYLMLFVLCVLCVSAAEAKLKKFTALQPPQKTVAYVTPTFDE
                     FTFRLPITARAAAPFYTLSVPAIVYQNARSTTLSDVAVFNAGKELVPYVVSENTQRTV
                     DTTEEQVSVPFFPLSFATADDSADVLLNVNKDERGTIISVGTSINVKSQTVTQVGSYL
                     LDLTTLTGTLDSFVINWKHKTQNETNKILISFSQDLLSWSSSHFGVLAHLEKDGETVV
                     VNRIDLPANSLPEGAKYARVTWEEGTAPIEITGITAGLSHHTTEQVVDE"
     gene            complement(805..>1280)
                     /locus_tag="VJC18_07655"
     CDS             complement(805..>1280)
                     /locus_tag="VJC18_07655"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HLD45295.1"
                     /translation="LNPIDLCMIGVFLSLFAWRFFLERQKLLSDKTHLHALGLVIWVF
                     MFFWLNATLLRSVHHWAGVAYTFDDLYASRIAQSAVSIYWALIAMVTMVYSSKKLLRH
                     VWLGGAVLLGLIVCKLVFVDLANRGTFERIVSFIGVGIVFLLIGFFSPLPKKLKE"
BASE COUNT          353 a          354 c          290 g          283 t
ORIGIN      
        1 ctcatccaca acctgttctg tggtatgatg ggacaacccc gcagtgatcc ccgtaatctc
       61 aatgggcgca gtcccctctt cccaagtcac tctcgcgtac ttcgcccctt ccggtaagct
      121 gttggccgga agatcgattc tgttgacgac aacagtctca ccgtcttttt ccagatgcgc
      181 cagcacccca aaatggcttg acgaccaact caagagatcc tggctaaagc taataagaat
      241 cttattcgtt tcgttttggg ttttgtgctt ccaattgatg acaaaactat caagcgtccc
      301 tgtgagcgtt gtcagatcca gcagatacga gcccacctgc gtgacggtct gagacttgac
      361 gttaatactg gtccccacac tgatgatcgt cccccgctca tccttattca cgttgagcaa
      421 gacatcggca ctgtcatcag ccgtcgcaaa agagagaggg aaaaaaggta ccgacacctg
      481 ctcctctgtg gtatccaccg tgcgctgagt gttttcactc acgacgtaag gcaccaattc
      541 cttgccagca ttgaatacag ccacatcgct cagtgtcgtg cttctggcat tctgatacac
      601 aatggcagga accgataagg tgtaaaaggg ggccgcggcc cttgctgtga tgggcaatcg
      661 aaacgtgaat tcatcgaacg tgggagtgac ataagccacc gtcttctggg gcggttgcag
      721 ggctgtaaat tttttcagct ttgcctcggc cgcagacaca cacaagacac ataacacaaa
      781 caacatgaga tattttttca ctgttcactc cttgagtttt ttgggcaggg gcgaaaagaa
      841 acctatgagg agaaacacaa tccccacacc aatgaacgac acgatgcgct caaatgttcc
      901 ccgattggcc agatccacaa aaactagttt gcaaacgata agccccaaga gcacggcccc
      961 acccaaccag acgtgtctca gcagtttctt ggacgaatag accatggtca ccatggcaat
     1021 cagcgcccaa tagatcgaca ctgccgattg cgcgattctg gatgcgtaca aatcgtcgaa
     1081 cgtgtaggcg acacccgccc agtgatgcac agagcggagc agtgtggcat tgagccaaaa
     1141 aaacatgaac acccagatca caagccccaa tgcgtgcaaa tgggttttat ccgaaagcaa
     1201 cttttgtctc tccaaaaaaa accgccacgc aaacagagac aaaaaaacac cgatcatgca
     1261 caaatcgatt ggattgagta
//
LOCUS       DATDUN010000547         1536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_441542, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000547 DATDUN010000000
VERSION     DATDUN010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441542"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VJC18_07660"
     CDS             <1..774
                     /locus_tag="VJC18_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HLD45296.1"
                     /translation="WNCGVTFTPKLEEGYAGNGLHFHLELVKNGKNVMVDTNGELSPE
                     CKKMIAGLCQHAPSLTAFGNTVSSAYLRLVPNQEAPTRICWSEQNRSALIRVPLGWRH
                     QCDMAKAVNPTQQESFDARGRSRQTVEFRSPDGSAHVNLLLAGLTLAVKSGLEDPHSL
                     KLAESLYVTKNIFKEKSNRLEELERLPVSCDGSADALINDKAFYQTDGLFPDEILDYV
                     YKKLKLENDKNINERFANLTADQRLEATRQIMHKDLHKQ"
     gene            complement(792..>1536)
                     /locus_tag="VJC18_07665"
     CDS             complement(792..>1536)
                     /locus_tag="VJC18_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45297.1"
                     /translation="NNIPKVDCKLKSIELTDSLTVQGNIGGEIAFPVKGLETLAKGHG
                     GASVSGDIKAGAKIEFDDQGNPTLVLRAGAGVQTAVEGKGGIGKYNRLVSSNRNDTSR
                     TNVWIENRYPIDKSALADFTSDPVGSVKKLVKEASVNKETVVTISSATDDLGKNTGTQ
                     QALVIRPVKVGDVFGGEVIEKIVSGDLEGVKQSLDEKKISNEYQTEDYEVCGDNLKGE
                     AGVPTKLKVEVGVAYGHKDVGGNNGLCAQ"
BASE COUNT          372 a          432 c          362 g          370 t
ORIGIN      
        1 tggaactgcg gggtcacgtt tacgccaaaa ctcgaggaag gctacgcggg taacggcctg
       61 cattttcatc tggaactggt gaaaaacggc aaaaatgtga tggtcgatac gaatggcgag
      121 ctcagtcctg agtgcaaaaa aatgatcgcg gggttgtgtc aacacgcgcc cagcctgacc
      181 gcgtttggca acaccgtatc cagcgcgtac ctgcgccttg tacccaatca ggaagccccc
      241 acgcgcatct gctggtcaga acagaatcgt tctgctttaa tcagagtgcc ccttggctgg
      301 cgacaccaat gtgacatggc caaagccgtc aacccgaccc agcaggaatc ctttgatgcg
      361 cgggggcgtt cccgtcaaac cgttgagttt cgtagtccgg atggctccgc gcacgtgaat
      421 ttattactcg cggggctcac gctcgccgtc aagagcgggc tggaggatcc ccatagttta
      481 aaactggcgg aaagtctgta tgtcaccaaa aacattttta aggaaaagag caaccgtctt
      541 gaggagctcg aacgcctgcc cgtgagttgt gacgggtccg ccgatgccct cataaacgac
      601 aaggcttttt accaaacgga tggattattc cccgatgaga tccttgacta cgtatacaaa
      661 aaactcaaac tggaaaacga caaaaacatc aacgagcgtt ttgccaatct cacagcggac
      721 cagcggctcg aggccacccg ccagatcatg cacaaggacc tgcacaagca gtgatggaaa
      781 ttcaacacag ctcattgcgc acacaaacca ttattgcccc ccacgtcttt gtgcccatac
      841 gccacgccca cctcgacctt gagtttcgtg ggcacaccgg cctcgccttt gagattatcg
      901 ccgcaaactt cgtaatcttc tgtttgatat tcattggaaa tctttttttc gtccaacgat
      961 tgtttgacac cttcgagatc cccggagaca attttttcga tgacctctcc cccaaacaca
     1021 tcaccaacct ttactggtct gatcacgagc gcttgttgcg tccctgtatt tttacccaaa
     1081 tcatctgttg ctgacgaaat agtcacaacg gtttctttgt tgacactagc ctctttgaca
     1141 agttttttga cagagccaac gggatcggag gtaaaatcgg cgagggcgga tttgtcgatg
     1201 ggataacggt tttcaatcca cacattggtg cgcgacgtat cgttgcggtt ggatgacacg
     1261 agacgattgt acttcccgat accccccttg ccctcgaccg ccgtctgaac ccccgcccct
     1321 gcccgcaaaa ccagtgtggg attaccctga tcatcaaatt ctattttggc acctgccttg
     1381 atatcgccgg aaacactcgc tccgccatgt cctttggcga gcgtttccag tcccttaacg
     1441 ggaaaagcga tttcaccacc tatattgccc tgaacagtca gactgtcggt cagctcaatg
     1501 cttttgagtt tgcaatcaac tttggggatg ttgtta
//
LOCUS       DATDUN010000548         1086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_68237, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000548 DATDUN010000000
VERSION     DATDUN010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68237"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VJC18_07670"
     CDS             <1..980
                     /locus_tag="VJC18_07670"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HLD45298.1"
                     /translation="SILKTIGQDNSLTSDRIYIVGYRYNQTLSNRSIIEAGGTINSHL
                     MLILGRYDGSWWGYHMFHDPNEPDKSPFRIDSLGESMPEMFDLVYIWEVLGTEMPRQG
                     TTHVMVQNMPPYQTVRPKIGWLGFLGLKKIQYFLDTIAVQLWSDGEQFPRVVVPQAGV
                     VDVVPPTHKGTRHSELLSFYNKVAIRHHSGTSMRGTYGLEYQCVEFVNRYYAKVFKHF
                     NMTGSGDADTYFYSAKYKNLVQFENEKSPEPPMVGDILVFDPPGTTDTNPGHVGIVYY
                     TDSKRVCLVQQNMDRWRGCLPLQNKNGAWYVGAVAPNLPCTGWTRKKGK"
BASE COUNT          308 a          269 c          261 g          248 t
ORIGIN      
        1 caagcatctt gaaaaccatt ggccaagaca acagcctaac ttccgaccga atctacattg
       61 tcggctaccg atacaaccaa accttgtcaa acagatctat tattgaagct ggtggcacca
      121 tcaactcaca cttgatgttg atcctcggcc gatacgacgg aagttggtgg ggctatcaca
      181 tgttccacga ccccaatgaa ccggacaaaa gtccatttcg cattgacagc ctcggcgaat
      241 caatgcccga gatgtttgac cttgtctaca tctgggaagt cttgggaaca gagatgccac
      301 ggcaaggcac aacccatgtc atggtgcaga atatgccgcc ataccaaact gtccggccaa
      361 aaatcggatg gcttggtttt ctgggtttga aaaaaatcca gtattttcta gacaccatcg
      421 cggttcaact ctggagcgat ggcgaacagt ttccacgagt tgttgttccg caagccggcg
      481 ttgtcgacgt cgtaccgcca acccacaaag gaacgcgaca cagcgaactc cttagttttt
      541 acaacaaggt cgcgattaga catcacagcg gcacaagcat gcgtggcacc tatggtcttg
      601 aataccaatg cgtagaattc gtaaatcgat actacgcaaa agttttcaag catttcaata
      661 tgaccggatc cggcgatgcg gatacttatt tctacagcgc aaaatacaaa aaccttgtcc
      721 agtttgaaaa tgaaaaatca cccgaaccgc caatggttgg cgatattcta gtctttgatc
      781 ctcctgggac cacagacaca aaccctgggc acgtcggaat tgtgtactac actgacagca
      841 aacgcgtctg tctggtccag caaaacatgg atcgctggcg cggatgtcta ccacttcaaa
      901 acaaaaatgg tgcgtggtat gtaggcgcgg ttgcgccaaa cctgccttgc actggatgga
      961 ctcgaaagaa aggaaagtaa aaatgaaggc aaagaatttg agaaattgtg ttctcgcggt
     1021 gtttctactg ttcctgacgg ctgggagcgg gggcgaaggc ggatgcgatt gcgatgactg
     1081 tgatac
//
LOCUS       DATDUN010000549         1142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_523558, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000549 DATDUN010000000
VERSION     DATDUN010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523558"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..713)
                     /locus_tag="VJC18_07675"
     CDS             complement(162..713)
                     /locus_tag="VJC18_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HLD45299.1"
                     /translation="MNILLGVTGSVAATLTPKLVQGFQVCDHTVQVVATQSSKYFFSP
                     SSLGVKVWLDGYEWLGVEYQKGQHIAHIDLTKWADVLVIAPLSANTMAKLANGLCDNL
                     PTCVFGAWDQTKPVVLAPAMNSNMWTNPVTRRHMQALSELVTYGLRLKFASPIEKTLA
                     CCDTGIGAMAEIHTIIDTVTSFS"
BASE COUNT          335 a          272 c          253 g          282 t
ORIGIN      
        1 gtgggtgcgg cgaaaagacc tcgaattgtt gaagactatt tcacaacttg caaacgtgat
       61 ctagccgcgc aatcggctca agactcacaa cagcacttct gaacttcgga agggtctttt
      121 aatttcaaac aaaaaaactc cggaaacaaa tccggagcag attaagagaa gcttgtgact
      181 gtgtcgatga tggtatgaat ttctgccatg gcaccgattc cagtatcgca acaggcaaga
      241 gtcttttcga taggtgaagc gaacttgagt cgaagcccgt aggtaacaag ctcactcaga
      301 gcctgcatgt gtctacgtgt gacaggattc gtccacatgt tggagttcat ggctggtgca
      361 agtaccacgg gtttggtctg atcccatgcg ccaaacacgc acgttggcag attgtcacac
      421 aaaccattgg cgagcttggc cattgtgttt gcactcagtg gtgcaatcac gagtacatcc
      481 gcccatttgg tcagatcaat gtgtgcgatg tgttgccctt tttgatattc gacacccaac
      541 cattcatatc cgtccaacca aaccttcaca cctaatgaac ttgggctgaa gaagtatttc
      601 gacgactgcg tagcaacaac ttgaacagta tgatcacaga cctgaaaccc ttgaacaagt
      661 ttcggtgtaa gcgttgccgc aactgaccca gtcacgccca agagaatgtt cattggaatc
      721 tcctaatgat cgcctctgcg atttggttgc gtgtgacatg ttcaggaacg ttgtctcgtc
      781 caatcacgat tgtgcgtttg cgtgcccaat caaggcaatt cgtcacgatt ggaaactcac
      841 acatgagcac acgcaagtct tcaaaaaaca aaaatgcacg tacgctcaat tccatgaaac
      901 gacaaaatac cctttgggtc aagcttgaca tttagttgtt ttatcgatag gttcgcatta
      961 tattctaaca aactccaagg ataaacaagc aaacaaagga tacacatgtt cagacaattg
     1021 gctgaaaaaa taggtcgact tctggctggc aagaagatgc caaaaggagc cagactctgg
     1081 ctctcaaatc agctcattca atttgccaaa accaacctga tctttgacga cacagggaaa
     1141 tg
//
LOCUS       DATDUN010000550         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_523570, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000550 DATDUN010000000
VERSION     DATDUN010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523570"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VJC18_07680"
     CDS             <1..543
                     /locus_tag="VJC18_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HLD45300.1"
                     /translation="AMGILKAIHIDTTGKTYDAYQTASALASVIKEGGYDLVLCGKQA
                     IDHDNASVPQMLAELMGAPQVTVVDKMEVAGNKVKVQRRVSGGAREVYDVTTPAVFSC
                     ERGLNTPRYASLPGIMKAKSKPIDKKQADAVLAGATPLVQFANYELPPERAAGKKLTG
                     EPAEQVEQLAKLLREEAKVI"
     gene            643..1623
                     /locus_tag="VJC18_07685"
     CDS             643..1623
                     /locus_tag="VJC18_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HLD45301.1"
                     /translation="MSDILIFVEHENGKVKKSTHELLSKATELAQSTGGTVSAVALGT
                     ALDGLTSDLASYGAKKVALVEDAKLAAYNTIAYTTALATVIENLKPGIVLGTASSMGK
                     DLFPRLSARIKAGFAQDCVGLRIDGGKLVAKKPIFAGKALVEITYISAVQMATIRANS
                     FAVNAPQAGATAEVFKLTAPAGDLINPLIEVIKGTGGDKVDLTEAETIVSGGRGLASK
                     ENFRILEDLANVLGATVGASRAAVDSGYASHDMQVGQTGKVVNPKLYIATGISGAIQH
                     LAGMRTSKVIVAINKDPEAPIFAKADYGVVGDLFKVVPLLTESLKKLLTV"
     gene            complement(1687..2553)
                     /locus_tag="VJC18_07690"
     CDS             complement(1687..2553)
                     /locus_tag="VJC18_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45302.1"
                     /translation="MGSINNYGCYDFEKGRVICPPHFEDALIAPVVLTPSLYVDDVAK
                     LNELVFQLELIDATDAASFTPKELEEAKKLLAETTFFASTANGYVPVATERSPLLVPE
                     AVTAMSLRRLLVHLKLFADSANRFERCGQSVTADEQALSQRVSSPWVVPSQPELHLFP
                     SEKGFWSKPPVLIPEEAFPDKGSWPMPWPSWPQTKATDPFWEFDPPTIITDPYWPSAH
                     KQELPTRTVNALIEGALVLTVGTDEEGRLTISAQQPHMIYEEVVHGENFPAIIYFEQS
                     QSSGDNAVWGDF"
     gene            complement(2522..2782)
                     /locus_tag="VJC18_07695"
     CDS             complement(2522..2782)
                     /locus_tag="VJC18_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45303.1"
                     /translation="MTRIVSINANQALLTGPQDESLIHKAFAEADDDGIISKTEIDQF
                     YHGSQYRQLTTDELASLDTIFGMRVTNNEGGYHGFYQQLWLL"
BASE COUNT          760 a          743 c          700 g          665 t
ORIGIN      
        1 gccatgggca tcctcaaggc catccacatc gatacgaccg gaaaaaccta cgatgcctat
       61 caaacagcca gtgctctggc ctctgtcatc aaggaaggtg ggtacgatct ggtgctctgc
      121 ggcaaacagg ccatcgatca cgataacgcc tcggtaccac aaatgctcgc cgaactcatg
      181 ggagcaccac aagtcacagt ggtcgataaa atggaagtgg caggcaataa ggtcaaggtg
      241 caacgcagag tctccggcgg tgcccgtgaa gtctacgatg tgactacccc cgctgttttt
      301 tcctgtgaac gcgggctgaa cacaccgcgc tacgcatcgc tacccggcat catgaaagcc
      361 aaatcaaaac ccattgataa aaaacaggcc gatgctgttt tggcgggtgc caccccactg
      421 gtgcaatttg caaattacga actccccccc gaaagagccg caggcaaaaa gctcacagga
      481 gaaccagcgg aacaggtgga acaattggcg aaattattaa gggaagaggc aaaggtgatt
      541 taaaaaacgt gttcacgtgt tcaggtgttt acgtgttctc gaaaaacatt ccttcgtgaa
      601 cacctgaaca catcaacacg tgaacacatt gtgaggtaat acatgtcaga catattaatt
      661 tttgttgaac acgaaaatgg caaggtcaaa aaatccacgc acgagttgct ctccaaggca
      721 acagaactgg cgcaaagcac tggtgggact gtatcagccg tagccttggg aacagcactc
      781 gacgggctca cctctgactt ggcaagctac ggtgccaaaa aagtggccct cgttgaggac
      841 gccaaacttg ccgcgtacaa caccatcgcc tacacaacag cgttggcaac agtgatcgaa
      901 aatctcaaac ctggcatcgt tttgggcaca gccagctcaa tgggcaaaga cctctttccc
      961 cgtctctcgg ctcgcatcaa ggcagggttc gcacaggatt gtgtgggtct gcgcatcgat
     1021 ggcggcaagc tcgttgccaa aaaacccatt tttgctggca aggcattggt cgaaatcacc
     1081 tacatctccg ccgtgcaaat ggcgaccatc cgtgctaatt cgtttgctgt gaatgctcca
     1141 caagctggcg cgactgctga agtgttcaag ttaaccgccc ctgctggtga tctcatcaac
     1201 ccgctgattg aggtcatcaa aggaacgggc ggcgataaag ttgatctcac agaggccgaa
     1261 accattgtct ctggcggcag aggattggcc agcaaagaaa atttcaggat tctcgaagat
     1321 ttggccaatg ttctgggagc cacagtgggt gcgtcccgcg cggcggtcga ttccggatac
     1381 gcctcacacg acatgcaggt gggacaaacc ggcaaggtgg tcaatcccaa gctctacatc
     1441 gccaccggca tttcgggagc cattcagcat ctggcgggca tgcgcacctc caaggtgatc
     1501 gtggccatca acaaggaccc ggaagccccc atctttgcca aagccgatta cggtgtggtg
     1561 ggtgatttgt tcaaggtcgt gccactactg actgaatcgc tcaagaagtt gctgacggtg
     1621 tgatgatcga tacttgtttt ttttatatat aaacccacta caaaacggtt tttttacaaa
     1681 gaaatctcaa aaatctcccc agacagcgtt gtcaccagaa gattgtgatt gttcaaaata
     1741 gataatcgca ggaaagtttt ctccatgcac aacttcctca tagatcatat gcggttgctg
     1801 ggcagaaatg gtcaaccgcc cctcctcgtc tgtccctacg gttaacacaa gggccccctc
     1861 tatgagcgca ttcaccgtcc tggtcggcag ttcttgttta tgtgcggacg gccaataggg
     1921 atcagttatt attgtcggag gatcgaattc ccaaaaggga tcagttgctt ttgtctgagg
     1981 ccagctgggc cacggcattg gccaagagcc tttgtctgga aaggcttctt caggaatcaa
     2041 aacagggggc tttgaccaaa accctttttc gctggggaac aaatgtaact caggttgaga
     2101 gggaacaacc caggggcttg agaccctctg cgacagggct tgttcgtcag cggtgaccga
     2161 ttggccgcaa cgttcaaacc tgtttgcgga atcagcaaac aatttaagat gcaccaaaag
     2221 ccgcctcaaa gacatggccg tcacggcctc cggaaccaac aagggactac gctctgtcgc
     2281 aactggcaca tacccgtttg cagtgctcgc aaaaaatgtg gtttcggcca ataatttctt
     2341 ggcctcttcc agttctttgg gtgtaaaact ggccgcatct gtcgcgtcga tcaattccag
     2401 ttgaaaaacg agctcattca acttggccac atcatccaca tagaggctgg gagttaacac
     2461 gacaggtgca atgagagcat cttcaaaatg agggggacag atcactctcc ccttttcgaa
     2521 atcataacaa ccataattgt tgatagaacc catgatagcc tccttcatta ttggttactc
     2581 tcattccaaa tatcgtatcc agactggcca attcatccgt ggtcagctga cgatactggg
     2641 aaccatgata gaactgatcg atttctgttt tggagatgat cccatcgtcg tcagcctcgg
     2701 caaaggcttt atggatcaag gattcatcct gaggaccggt cagcaaagct tgattcgcat
     2761 tgatggatac gatgcgcgtc atgttgccat actttcactt tcgccttttt ctacggtccc
     2821 attatcggtc atcgaaaaaa aatgttgcta acaaaatatg cctttttt
//
LOCUS       DATDUN010000551         3006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_231659, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000551 DATDUN010000000
VERSION     DATDUN010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231659"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VJC18_07700"
     CDS             <1..586
                     /locus_tag="VJC18_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45304.1"
                     /translation="PEVINSLEAQVGEVNFDTLYAAAGDEFNGYITSLIEDDPELMAL
                     FATQAGGEGEGDWVMGYLSDLAANTDMGDSNQGSLTDDARALADMYGLGEGFDWVINQ
                     EDLFASAEMAILDRLAEYDQAMADLVAALESGALSDTQFATEVQNCQFAREVDIALLQ
                     QMTDTKMSLFETISKIYNQTFEMQKSIINNIRVS"
     gene            605..1273
                     /locus_tag="VJC18_07705"
     CDS             605..1273
                     /locus_tag="VJC18_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD45305.1"
                     /translation="MDETKLIPTISSKNKKIDPDKIKSKLLSPNPKRIKYITDILAAL
                     SENKITFASVTRMDRKKMRSLAEQGYIKLKHGRYDEARKIFEILTFVDHKNYFHHLAL
                     GGAYHKMKKYLDAAFQYGECLKYDSENINALVNRGEIFLKHKNYKKAAEDFRSAILLD
                     KTGRDVFSNRARSLVIAIRRSLARDKGKQNVALPENPSKRKKLKPAVLMSKKPTKQKK
                     FIGR"
     gene            1379..2155
                     /locus_tag="VJC18_07710"
     CDS             1379..2155
                     /locus_tag="VJC18_07710"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HLD45306.1"
                     /translation="MIKNLFIGLGYNFKGFGFFITNRTLWKFAIIPLFINLLALILLI
                     ALYVSHFGDIFAFITKPLGSLDITAPNGFFWHLADGLFWFLRFGLKIVFFALSFVLIF
                     MLVFILSGLVNAPFYELLAEQVLTLKNEWVERPFQLKSFLTEMGHSLKIELCKLVLFV
                     ALSLMILVLSSVPVIGVFFGLIGIVFAAWFFAFGLSSFPLVLQRKSFKDILGWAWQNN
                     MLLTGLGLPATIPVVGMLFLHFQVIGGTLLYIDHKQPEHL"
     gene            2191..2619
                     /locus_tag="VJC18_07715"
     CDS             2191..2619
                     /locus_tag="VJC18_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45307.1"
                     /translation="MLLLLPQACNRTPPERALMKHFETENALTVQFQNNYIGYMEYQL
                     AHFSEKRAQLEGLFRTINSPFNKMTTVEQQAYQRHWQTLMQPLVDSLYANTRLMVMNE
                     KEKATPAKLAKINELTIKMNLLESAAPNQQLLPQLFGTPK"
     gene            2701..>3006
                     /locus_tag="VJC18_07720"
     CDS             2701..>3006
                     /locus_tag="VJC18_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLD45308.1"
                     /translation="MKVRQAVIPAAGLGINFLPITKTIPKELLPIIDKTCLEYVFAEG
                     ISAGVEEFIVVISKEKQIIRDYFEDSQYMIDWLRNRHLDNLIPTLNPLPKNIKLTFVY
                     "
BASE COUNT          821 a          793 c          650 g          742 t
ORIGIN      
        1 ccccgaagtg atcaacagcc tggaggcgca ggtcggtgaa gtcaatttcg acaccctgta
       61 cgccgccgca ggagatgagt tcaacggcta catcacctca ctcattgagg acgatcccga
      121 gttgatggcg ctctttgcca cacaagcagg gggtgaaggg gaaggcgact gggtgatggg
      181 ttatttgtct gacttggccg ccaacaccga catgggagat tccaaccagg gttcactcac
      241 cgatgacgca cgcgccctag ctgatatgta cgggctgggg gaaggtttcg actgggtgat
      301 taatcaggaa gacttgtttg cttcggccga aatggccatt ttagacaggc tggccgagta
      361 tgaccaagct atggccgacc tcgtggccgc actcgaaagc ggcgctctga gcgacacaca
      421 atttgcaacc gaagttcaaa actgtcaatt tgcccgtgaa gtcgatatcg ctttgttgca
      481 acaaatgaca gatacgaaaa tgtccctgtt cgagacgatt tccaaaattt acaatcagac
      541 cttcgaaatg caaaaatcga tcatcaacaa catacgtgtg tcttaagacg cttttgaact
      601 cgctatggac gaaaccaagc tcataccaac catctcttcg aaaaacaaaa aaatcgatcc
      661 cgacaagatc aagtccaaac tgttatcccc caatcccaaa cgcataaaat acatcaccga
      721 tattttggcc gcgctgagcg aaaacaaaat cacctttgcc tctgtgacac gtatggatcg
      781 caaaaaaatg cgttcgctcg ccgagcaagg ttatatcaaa ctcaaacacg gccgctatga
      841 tgaggcccgc aaaatcttcg aaatcctcac gttcgtcgac cacaaaaatt attttcacca
      901 tttggccctg ggcggtgcct atcataaaat gaaaaagtat ctggacgccg cttttcaata
      961 tggtgagtgc ctcaaatacg attccgaaaa catcaacgcc ctcgtgaaca ggggcgaaat
     1021 ctttttaaaa cacaaaaact acaaaaaggc cgccgaagac ttccggtcag ccattctgct
     1081 cgacaaaacc ggccgtgatg tcttttccaa ccgggcccgc tctctcgtca ttgccatcag
     1141 acgctctttg gccagggaca agggcaaaca aaatgtggcc ctgcccgaaa atccctcgaa
     1201 gagaaaaaaa ctcaaaccag ctgtcctcat gtccaagaag cccaccaaac aaaaaaaatt
     1261 cattggcaga tgaatggcag tccctgaaaa atcctcgtaa cgtcatggcg atgcgcggca
     1321 atccacttgt tttgcaatga caaatacgca aagaaaacat atacagggcg gggggggtat
     1381 gatcaaaaat ctttttattg gcctgggcta caattttaag ggtttcggtt ttttcataac
     1441 aaaccggaca ctgtggaagt ttgccatcat cccattgttc atcaatctgt tggccctcat
     1501 attgctcatc gccctgtacg ttagccactt tggcgacatc ttcgcattca tcaccaaacc
     1561 cttgggctcg ctcgacatca cggccccaaa cggttttttc tggcacctgg cggacgggct
     1621 cttctggttc ttgcgttttg gtttaaaaat cgtttttttc gccctttcgt ttgtcctcat
     1681 ttttatgctc gtgttcatct taagcggttt ggtcaacgcg cccttttacg aactcctggc
     1741 ggaacaagtc ctcacactca aaaacgagtg ggtcgaaaga ccctttcaac tgaagtcatt
     1801 tctcacagaa atgggtcact ccctgaagat cgaattgtgc aaattggttt tgtttgtagc
     1861 cctctccttg atgatcctcg tcttaagcag tgtccctgtc attggggtct tttttggctt
     1921 gattggaatt gtcttcgccg cctggttttt cgcttttgga ctcagctctt ttccgctggt
     1981 ccttcaaaga aagagtttta aagacatctt aggctgggcg tggcagaata acatgttgct
     2041 cacaggtttg ggccttccgg cgaccattcc tgtcgtcggg atgctcttcc tccattttca
     2101 ggtcattggt ggcaccctgc tgtacatcga ccacaaacaa cccgaacatt tatgagctcg
     2161 tccagatctc tcacaggcct ccttgccctt ttgttactcc ttctgccgca ggcctgcaat
     2221 cgcacgccac cagaacgcgc gcttatgaag cattttgaga cagaaaatgc gctcaccgtg
     2281 cagtttcaaa acaactacat cggttacatg gaatatcaat tggcccattt ttcggaaaaa
     2341 cgcgcacagt tggaggggct cttcaggacc atcaactccc ctttcaacaa aatgaccacg
     2401 gttgagcaac aagcgtatca gcgccattgg caaacactca tgcaaccact ggtcgactcg
     2461 ctttacgcca acacccgttt gatggtcatg aatgaaaaag agaaggcaac accagcaaaa
     2521 ctcgccaaaa tcaatgaact cacgatcaaa atgaacctct tggaaagtgc tgcgcctaat
     2581 caacaactcc tcccccagct ctttggcaca ccaaaataaa tggaattgaa ggctgaccgg
     2641 tcacacaaca tggtagacag attttttaaa gctgatatga agaggcaggg ggaatcttct
     2701 atgaaggtca gacaagccgt cattccagca gccggactgg gcatcaattt tctgcccatc
     2761 accaaaacaa ttcccaaaga gttgctcccc atcatcgaca aaacctgtct ggaatacgtt
     2821 tttgccgagg gaataagtgc cggtgtggaa gagtttatcg tcgtcatctc caaagaaaag
     2881 cagatcatca gggactattt tgaggacagt cagtatatga tcgactggtt gagaaaccgt
     2941 catttggaca acctgatccc caccctcaat cccctgccca aaaacatcaa actcacattt
     3001 gtttat
//
LOCUS       DATDUN010000552         5166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_150660, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000552 DATDUN010000000
VERSION     DATDUN010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150660"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VJC18_07725"
     CDS             complement(<1..581)
                     /locus_tag="VJC18_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HLD45309.1"
                     /translation="MKDFTCPYGTHRVISPEDALPQNAQKIDNSLPIKSNEILISVDT
                     LNVDAASFRQMSEVCQGDSEGIKKLILKTVSERGKQHNPVTGSGGMLLGTVKEIGPKY
                     SNKAGVKVGDKIATLVSLSLTPLYIERIKEVFHGRDQVKIDGYAILFDSGVLAKIPDD
                     MPEALVLAVLDVAGAPAQAMRLVKPGQTVVILGG"
     gene            complement(688..1941)
                     /locus_tag="VJC18_07730"
     CDS             complement(688..1941)
                     /locus_tag="VJC18_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HLD45310.1"
                     /translation="MSHVVKKPELLIPAGCLERLKYAFAYGADAVYAGAPQFSLRVRE
                     NSFSEENIMEGIAYAKEHNKKFYLTVNVLAHNRKIKPFINKLEAMIAARPDALIMADL
                     GLIDIVRNSYPDMPIHVSVQTNTMNWAAVQAWKKMGVTRIILSREVSIEEMKEIRQRV
                     PDIELEAFVHGAVCVAHSGRCLLSNYFSYRDANDGCCTNACRWKYRLREQKTHRYFLQ
                     EAHRPDDCLEIEEDEQGTYILNAKDLRAVEHISELVAAGVDSMKVEGRNKSLYYVAVV
                     TRAYRKAIDDVMAGKSFDAGLIAELNKIHNRGYTSGFLVTRAGQESIRYDEGLTNLYT
                     QEFSAVILDQKPGHILVTPRNRLCVGDAVEIMTSQQSFRCRVEGLVTEDGLSVAAIHG
                     GTDVRVWMPYEGGPLPPFSIISKIV"
     gene            complement(1938..2624)
                     /locus_tag="VJC18_07735"
     CDS             complement(1938..2624)
                     /locus_tag="VJC18_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLD45311.1"
                     /translation="MMQATVSAQKNKKKILIVDDHPIIRHGLRKVIDQEIDLCVCAEA
                     EDAVQAMEAIGKACPDLVIVDLSLKGTNGLDLIKAIKAKQDLAVIVLSIHDETIYAER
                     ALRAGAKGYVMKQEAADQLLHAIRRVLEGDIFVSDKMSKYLLQKYLDGGANAEHTSPV
                     SKLSDREIEIFRLIGEGRSTRKIAEELSLSVKTVESHRAHIKQKLRMTSGTELVHNAV
                     QWVQSEKGTT"
     gene            complement(2658..4067)
                     /locus_tag="VJC18_07740"
     CDS             complement(2658..4067)
                     /locus_tag="VJC18_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLD45312.1"
                     /translation="MPNKNTSNTTKVARTKHTSSQALEKHGIGFDDFVNSLTDYVTVV
                     DTEYNIVFANKVVLRTAPHKIMGTSVFLYVPEDEQEKVKQKLDGVMSNRQPVGHDLRS
                     NVPGVGAVWFSGVASPIIKRGRVVGIVIVARDVTTKKNYEETLLRDILDKKRCIPLEH
                     RIPDEIFLVNREGRFLDFSLTRDFEPYVPPESFIGKRLDEVMPQEVALRALKILKEAF
                     ETKIAQIYEYELTQNGAKRHFEARFIVMHDDECMIIVRDTTDTLGLREEFLKLVSEEQ
                     KRMGHNLHDGLSQHLTGIAFLGKDLENRLKNRGLKEAGDAKKITHLVNEAIDHTRRLA
                     QGMHLVEIETSELKDVLAELCTTTEKIYRIACRLEYDNGVQFDDVDVKMHIYRIVQEA
                     INNAIRHGGAKAIDIKVRKHPEGVRFVVADNGNGIENNFEAHGAGFKIMKYRAQMINA
                     TMRVKSQLGTGTQISFDMF"
     gene            4178..4651
                     /locus_tag="VJC18_07745"
     CDS             4178..4651
                     /locus_tag="VJC18_07745"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HLD45313.1"
                     /translation="MVIYDLICSLNHKFEAWFRDQASFEKQLQQGLVQCSVCGNSAVR
                     RTPSGCHVSKTPQTLSPSTKRASEATVSTRQHPTAQKTQNIDPVVLLKTMHKYIKDNF
                     QDVGPQFADRVIQMHEGAVPFASVYGTTDQEQRDRLDDAGISYVPLPLLPESLDN"
     gene            complement(4700..>5166)
                     /locus_tag="VJC18_07750"
     CDS             complement(4700..>5166)
                     /locus_tag="VJC18_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45314.1"
                     /translation="DPAEILGETTEGGLGVFAAAPTYTLPTEPPSVVQELDFGKEEGF
                     YFLVSCDGNFLGKLSQDAEDDLSVYNEDSDYASDSSDFSIFNPWSVYGRSFTACSAFN
                     EDASYPPQIYFRSFSDVQDYFVGYLTTNNTLDYQKNTCQTLESLSIFYDGCL"
BASE COUNT         1284 a         1375 c         1063 g         1444 t
ORIGIN      
        1 cctcccaaga tcaccacagt ctgcccgggc ttgaccagcc gcatggcctg tgcgggggct
       61 ccggccacat ccaacacagc caacaccaag gcctccggca tgtcatcggg aattttggcc
      121 agaacgccag aatcaaataa aatggcatac ccatcgatct tgacctgatc cctcccgtga
      181 aagacttcct ttattcgttc aatgtacaaa ggggtgagcg acaatgacac cagcgtggca
      241 attttgtctc cgaccttcac gcctgccttg ttagagtatt tggggccaat ctctttgacg
      301 gttcccaaga gcatgcctcc agaacccgtc acaggattgt gttgcttgcc ccgttcggaa
      361 accgttttta atatcaactt tttgatgcct tcactatcgc cttggcaaac ttcagacatt
      421 tgtcgaaatg acgccgcatc aacattcagc gtgtccacac tgatcagaat ctcatttgac
      481 ttgatcggca gtgaattatc aattttttgc gcattctgcg gcaaagcatc ttctggtgaa
      541 atgacccgat gcgttccata gggacaggta aaatctttca tacaaagctc ctctcgattt
      601 cttgatgaat ggttgtgact tgtgcctcgg ctttaaaata attgtgtgcg ggttggcaac
      661 agattttgat ggggcatcac accacgttta tactattttg gaaatgattg agaaaggcgg
      721 caggggccca ccctcatagg gcatccagac cctgacgtcg gtgcctccat gtatggcagc
      781 taccgacaaa ccatcttcgg tcacaagccc ctcaacccga caccgaaagg actgttgcga
      841 cgtcatgatc tccacagcat cacccacaca tagcctgttt ctgggcgtca caaggatgtg
      901 cccaggtttt tgatcgagaa tgacggccga aaattcctgc gtgtacaaat tggtcaagcc
      961 ctcatcgtat ctgatgcttt cttgcccggc gcgtgtcacc aaaaaacctg aagtgtatcc
     1021 tcgattgtga attttattta gctccgcgat caagcccgca tcaaatgatt ttcccgccat
     1081 cacatcatct atggccttgc gataggccct ggtcaccacc gccacgtaat aaagtgactt
     1141 gttgcgccct tccactttca tgctgtcaac accggcagcc acaagctcag aaatgtgttc
     1201 cacggcacgt aagtctttgg cgtttagtat gtatgtgccc tgctcgtctt cttcaatttc
     1261 caaacaatca tcggggcgat gggcctcctg taaaaaatac ctgtgcgttt tttgttcccg
     1321 taatcggtat ttccacctgc aggcattggt acagcaacca tcattggcat cacgatagct
     1381 gaaatagttg gacaaaagac acctgcctga gtgggccaca caaactgccc cgtgaacaaa
     1441 ggcttccaat tcaatatcag gaaccctttg acgaatctcc ttcatttcct caatggagac
     1501 ttcgcgcgac aaaataattc tggtcacacc catttttttc catgcctgta cggcggccca
     1561 attcattgtg ttggtttgta ccgaaacatg gatgggcata tcgggatagc tattccggac
     1621 aatgtcaatc agccccaaat cggccatgat caatgcgtct ggccttgcgg caatcatcgc
     1681 ttctaactta ttgatgaacg gcttgatttt gcggttgtgc gccagaacat taaccgtcaa
     1741 atagaatttt ttattgtgtt ctttggcata ggcaatgccc tccataatat tttcttcaga
     1801 aaaggaattt tcgcgaaccc tgagcgaaaa ttgtggggcc cccgcataca ctgcgtccgc
     1861 accatacgcg aacgcgtact ttaatctctc aagacatccc gcgggaatca atagctctgg
     1921 ttttttcaca acatggctca cgtggtccct ttttcacttt gcacccactg cacggcgttg
     1981 tgtaccaact ctgtcccgct ggtcatcctg agtttttgtt tgatgtgtgc cctgtgggat
     2041 tcaactgttt tgacactcaa agacagttct tcggcgattt ttctggtgct tcgcccttcg
     2101 ccgatcagcc tgaaaatttc tatttcccga tcactaagct ttgagacagg agatgtgtgc
     2161 tcggcattcg caccaccatc caaatatttt tgcaacaaat atttgctcat tttgtcgctg
     2221 acgaaaatat ctccttccag cacgcgacga atggcgtgca acaactggtc ggccgcttcc
     2281 tgtttcatca cataaccctt ggcccctgca cgcaacgccc gctcggcata gatcgtctcg
     2341 tcatggatag aaagaactat caccgccaag tcctgttttg ccttgatagc cttgatcaga
     2401 tcaagcccgt tggttccttt gagagacagg tccactatca ccaaatccgg acaggccttg
     2461 ccaatggctt ccatagcctg tacagcatcc tcagcctccg cacagacgca caggtcgatt
     2521 tcctgatcaa tcacctttct cagtccgtgt cgaattatcg ggtggtcatc aacaatgagt
     2581 atcttttttt tgtttttttg cgcgcttact gtggcttgca tcatttgtcc tcctttaagg
     2641 gtactgcgaa gaccccatta aaacatatca aaactaattt gggttcccgt tcccagctgt
     2701 gatttaaccc tcatcgtcgc attgatcatc tgtgcccgat acttcatgat cttaaacccc
     2761 gcgccatgcg cctcaaaatt attttcaatg ccgtttccat tatccgcaac cacaaacctg
     2821 actccctcag ggtgttttct gactttgatg tctattgcct ttgcgccacc gtgcctgatt
     2881 gcattattga tcgcctcttg gacaatgcga tagatgtgca ttttcacatc cacgtcatcg
     2941 aactggacac cgttatcata ttccaggcga caggcaatcc tgtaaatttt ttctgttgtc
     3001 gtgcagagtt cggccaacac gtctttcaat tcactggttt cgatttcgac cagatgcatg
     3061 ccctgagcca gccttcgcgt gtgatcaatg gcttcgttga ccaagtgcgt aattttcttg
     3121 gcatcacccg cctctttgag acccctgttt ttcaatcggt tttccaagtc ttttcccaga
     3181 aaagcaattc ccgtcaggtg ttgcgataac ccgtcatgca aattgtgacc catccttttc
     3241 tgttcttccg acaccaactt caaaaattct tccctcagac ccagggtgtc tgtcgtgtct
     3301 ctcacgatga tcatacactc atcatcatgc atgacaatga atcgtgcctc aaaatgccgt
     3361 tttgcgccat tttgggtcag ctcgtactca taaatctgcg caattttggt ttcaaaagct
     3421 tccttcaaaa ttttaagagc cctcaaggcc acctcttgag gcatcacttc gtctaatctt
     3481 ttcccaatga aagactcagg gggtacatag ggttcaaaat ctcttgtgag actgaaatcc
     3541 aaaaaacgtc cttcccgatt aaccagaaaa atttcatcgg gaatcctatg ttccaaggga
     3601 atgcatcttt ttttgtcgag aatatcacgc aacaatgttt cttcgtaatt tttctttgtt
     3661 gtcacatcac gagccacaat cacaatacca acaacccttc ctcgtttgat gatcggagag
     3721 gccacgccag aaaaccacac agcccccacg ccaggaacat ttgaccgcaa atcatgtccg
     3781 actggctgcc tgttgctcat aaccccatcc agtttctgtt tgactttctc ttgctcgtct
     3841 tcgggcacat acagaaacac gctcgtcccc atgattttat gtggggccgt tcgcaagaca
     3901 accttgtttg caaaaacaat attgtattct gtgtcaacaa ccgtcacata atcagtcaga
     3961 ctgttcacga aatcatcaaa acctattccg tgtttttcaa gggcttgtga gctcgtgtgt
     4021 ttggttcgcg cgaccttggt cgtgttgcta gtgtttttat tgggcacaaa aaattgtcta
     4081 cacccattga aaacacaaaa caatagaaga ctatgtgaca gggcctcctt gacaaacttg
     4141 ataaaaagat taaatttgga tcacttttag agactttatg gttatttacg atctcatttg
     4201 tagcctcaat cataagtttg aggcctggtt cagggatcag gcgtccttcg agaaacaact
     4261 gcaacagggg ctggttcagt gttctgtttg cggaaattct gctgttcgac gcacgccctc
     4321 tggttgtcat gtttcaaaga cgccccagac tctctcccct tcaacaaaga gggcctctga
     4381 agcaacagtc tccacacgac aacacccgac agcacaaaaa actcaaaaca ttgaccctgt
     4441 cgttctcctc aaaacaatgc ataaatacat caaggacaac tttcaggatg tcgggccgca
     4501 gtttgccgat cgcgtaatac agatgcacga aggagcagtt cccttcgcat cagtctatgg
     4561 cacgactgat caagaacaac gcgacagact tgatgatgcc ggaatttcgt atgtgccgct
     4621 acctctgttg ccagaatccc ttgataatta gagactggca cgaagctttc ttgaaattgt
     4681 aaaatgtatt gccgcggggc taaagacatc cgtcatagaa tattgaaagg ctttctagcg
     4741 tttgacaggt gtttttttga tagtccagcg tattatttgt agtgaggtag cccacaaaat
     4801 aatcctgcac atctgaaaaa gaacggaaat agatctgggg ggggtatgaa gcatcctcgt
     4861 tgaaggccga acaggctgta aacgaacgcc catacacgct ccagggatta aagattgaaa
     4921 agtctgaaga gtcgctcgca taatcactgt cttcgttgta aacagacaaa tcatcttcgg
     4981 cgtcttgcga taattttccc aaaaaatttc cgtcacagct aaccaagaaa taaaagcctt
     5041 cttctttgcc aaaatccaat tcctgaacga ccgagggggg ctctgttggc agtgtgtatg
     5101 tcggcgctgc tgcaaaaaca cccagccctc cctctgtcgt ctcgcccaaa atttctgcag
     5161 gatcgc
//
LOCUS       DATDUN010000553         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_29638, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000553 DATDUN010000000
VERSION     DATDUN010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29638"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1288)
                     /locus_tag="VJC18_07755"
     CDS             complement(116..1288)
                     /locus_tag="VJC18_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45315.1"
                     /translation="MKRILTTMIVFALLIGGCTGADQLPINAADISGTMDVGTIVQNP
                     KPPSEVILQIFPDFQTNLPGLSQMSNTDIEHIIVPDSTERMNTILMLGKIFNADNIRN
                     FDVSALFLAIDSLGTILKKTLADNSIALPVTTSTSVTLSENARLLSLDQYQWIVRLEP
                     EVDYPDYTRMYFIHPESHQILASYVFKNDSTGKVEKGMLGVAFPESLSSQNEHRGKIR
                     VSVLSFDFSDQAQNLFNISMDRKQNPQLSQSGQLFVQCNLTTSSCVGEQQSIISEPPT
                     RTFANPMPRYQWQSNNLDVCFGEMKYQADTAALNNVLLYEGNTHTVTKEACTIATPIW
                     GTHVFTPDDLFIRIEDTVSESTFNAIFGDGQSYDSWQQFISPALIEQTLDGSNQKI"
     gene            complement(1285..>2165)
                     /locus_tag="VJC18_07760"
     CDS             complement(1285..>2165)
                     /locus_tag="VJC18_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD45316.1"
                     /translation="FMAIYKKSVIIKIMVSIFDYKDYRIFLKDYYLEKKQLIRRFSYR
                     YFAKKAGIKGQSYLDLVIQGKRNLTKITLEKFSRALELNKKEASYFDTLVHFNQAKND
                     DERNMYFEKLIKQNPRKGFSEIDKKRYLYFTNRHLITIREMVSLPHFEENPDWISQHM
                     RHPLPPPKIIEAINLLIKMNLLKRDSQGKLRLSQGAVTTQPDFESLEALNYYHSIFQD
                     AKEAIINIPFDKREAKSLTIPMSEKLIPKIKEKIQNFIEDIVRLVNNECDSFDDVYQV
                     NTVFFPVTKTGGEKIK"
BASE COUNT          529 a          404 c          568 g          664 t
ORIGIN      
        1 attacccaac gcggaatcaa gtcgatatca gatgaggtcc agttcaccat ggatctgcga
       61 tttggggtgg ggtctggagg atgagttctt gcggatcaaa ctggcatgag ggcacctata
      121 ttttctggtt tgagccatcc agtgtttgtt caatcaatgc ggggctgata aactgttgcc
      181 acgagtcata ggattgcccg tcaccaaaaa tggcattaaa agtgctttca ctcacggtat
      241 cttcaatgcg gataaacaaa tcgtcgggag taaacacgtg ggtcccccaa atgggtgtgg
      301 cgatggtgca ggcctcttta gtaacagtgt gtgtgtttcc ctcatacaat aaaacattgt
      361 tcaatgcggc agtatcagct tgatatttca tttcgccaaa gcaaacgtcc agattgttgc
      421 tttgccactg gtaccggggc atggggttag caaaggttcg ggtagggggt tccgagatga
      481 tgctttgttg ttcaccaaca caggaactgg ttgtgagatt gcactgcacg aataattgtc
      541 ctgactggga cagttgggga ttctgtttac gatccatgct tatgttgaac aggttttgtg
      601 cctggtcaga aaaatcgaaa gacaacacac taaccctgat ttttcctctg tgttcgttct
      661 gtgatgaaag actttcgggg aaagcgactc cgagcatgcc tttttcgact ttgccagttg
      721 aatcattttt gaagacgtag cttgccagaa tctggtgaga ttcgggatga ataaaataca
      781 tgcgggtata atccggataa tcaacttcgg gttccaatcg aacaatccat tggtattggt
      841 cgagcgatag caaccgggcg ttttccgata aagtgacgct ggtggatgtg gtcactggta
      901 gggcgatgct gttgtccgca agcgtttttt tcagtatggt gcccagtgag tcgatggcca
      961 gaaacagggc agacacgtca aagtttctga tgttgtcagc attgaaaatc tttcccaaca
     1021 tcaaaatagt gttcattcgc tcggtactgt ctggcacgat gatatgctcg atgtccgtgt
     1081 tgctcatttg ggacaagccg ggaaggttgg tttggaagtc gggaaaaatc tggaggatga
     1141 cttctgaggg gggttttgga ttctggacaa tggtcccgac gtccattgtg cccgaaatat
     1201 cagccgcatt aatggggagt tggtcggcac cggtgcaacc gccaatcagc aaggcaaaca
     1261 cgatcatggt tgtgagaatg cgtttcattt gattttttct cctcctgttt tggtgactgg
     1321 gaaaaagact gtattgacct gatagacatc atcaaaagag tcgcattcgt tgttgaccag
     1381 cctgacgatg tcttcaatga agttttgaat tttttctttg atttttggta tcagtttttc
     1441 tgacatgggg atcgtgagtg atttggcttc acgtttgtca aagggaatgt tgatgatggc
     1501 ctccttggcg tcctgaaaaa tggaatggta atagttcagg gcttccagtg attcgaagtc
     1561 gggctgggtt gtgacagcgc cctgcgatag cctcagtttg ccctgcgaat cgcgttttaa
     1621 cagattcatt ttgatgagaa gattgatggc ttcgatgatc ttcggagggg gaaggggatg
     1681 gcgcatgtgt tgactgatcc agtcaggatt ttcctcaaaa tgcggcagag aaaccatttc
     1741 gcggattgtg atcaggtgcc tgttggtaaa atagaggtat cgttttttat caatttcaga
     1801 aaagcctttg cgaggatttt gtttgattaa tttttcgaaa tacatgtttc gctcatcgtc
     1861 gtttttggcc tgattaaaat gcacgagggt atcaaaatac gatgcttctt ttttgttgag
     1921 ttccagagcc cggctgaatt tttccagggt tattttggtt aaattgcgtt tgccttgaat
     1981 gacgaggtca agatagcttt gccctttgat gccggctttt ttggcgaagt aacggtaaga
     2041 gaaccggcgg attaattgct ttttttcgag ataataatct tttagaaaaa tgcggtaatc
     2101 tttgtaatca aaaatactga ccattatctt aattataaca gattttttgt agatggccat
     2161 gaatt
//
LOCUS       DATDUN010000554         1245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_29746, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000554 DATDUN010000000
VERSION     DATDUN010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29746"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..>1245
                     /locus_tag="VJC18_07765"
     CDS             47..>1245
                     /locus_tag="VJC18_07765"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLD45317.1"
                     /translation="MVSIYKFGIVEDHPYIVYEFVQGTPLDQIQSPVPSEKLIKIGVD
                     IASGLAAAHRNGVIHRDIKPANAIITADDTTKILDFGIAKVLSQKELLVADQEKGALS
                     INTPSAIREDISEDIISVSTRRWDENQGGSVKIQEAGTSLTHPGGIVGTPQYMAPELF
                     LGATANFQSDVYSFGALMYTLCTGVPPHKGKNLLDIISDIQNNEPCPIADLAPEVDSR
                     IKQIIQKCLARDPKERFPSGSELRSAWSRLSLFVTELSHHQDVMNPFRGLDVFEADHQ
                     QLFFGRDSEIRMLLEKLKRDPFILIVGDSGVGKSSLCRAGVLPRVGSWLHEKNSWEIV
                     RLVPGRNPIQALAKALVPVLHLSDIEIQKTIKDEAQGIGRFIRAHLPEQTGLVLFIDQ
                     LEELVTLG"
BASE COUNT          302 a          294 c          308 g          341 t
ORIGIN      
        1 gttttttatc gaggcacgtg ctattgcacg actacaacat ccgaatgtgg tttctatcta
       61 caagttcggc attgtggaag atcatcccta tatcgtgtac gaatttgtgc agggcacgcc
      121 cctcgatcag atccaaagcc ctgtcccaag cgaaaaactg attaaaattg gtgtagatat
      181 cgctagtggg ttggccgcgg cccatcggaa tggcgtgatc caccgcgata ttaagcctgc
      241 caatgccatc atcactgcag acgatacaac caagattctt gattttggca ttgctaaagt
      301 tttgtctcag aaagagcttc tggttgccga tcaagagaag ggagctctga gtataaacac
      361 tccgtctgca attcgtgagg atatatctga agacatcatc agtgtttcga ctcgacggtg
      421 ggatgaaaat cagggcggtt ccgtaaaaat tcaagaagcc ggcacctcat taacccatcc
      481 tgggggaatt gttgggacac ctcaatacat ggccccggag ctttttctag gcgccacggc
      541 caattttcag tctgatgtct attcttttgg ggcactcatg tatactttgt gcacgggtgt
      601 tcccccgcac aaaggcaaga acctcctcga cattatctct gacattcaaa acaatgaacc
      661 ttgtccgatc gcagatttgg cacccgaggt tgattcgcgc atcaaacaga tcattcagaa
      721 atgtcttgcg cgtgatccca aagagcgatt cccctctggt agtgagcttc gctcggcttg
      781 gtcgcgtctt tcgcttttcg taacggagtt gtcacatcat caagatgtga tgaatccatt
      841 tcgaggactg gatgtctttg aagcggatca tcaacagctt ttttttggtc gggattcaga
      901 aatccggatg cttcttgaaa agctcaaacg agatcctttt attctcattg tcggggactc
      961 gggcgtcggg aaatcatctc tgtgtcgtgc gggtgttttg ccgcgagtcg gatcttggct
     1021 tcatgaaaaa aattcctggg aaatcgtacg attggtgcca ggacgaaatc cgattcaggc
     1081 ccttgccaaa gcgcttgtgc ctgtgctcca cctgtcggac atcgaaatcc aaaaaacgat
     1141 taaggatgaa gcccaaggca ttggacgctt tatccgagcg catttgcctg agcaaaccgg
     1201 tttggtgctt tttattgatc agctggagga gcttgtgact ttggg
//
LOCUS       DATDUN010000555         3912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_398809, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000555 DATDUN010000000
VERSION     DATDUN010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398809"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VJC18_07770"
     CDS             <1..1175
                     /locus_tag="VJC18_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD45318.1"
                     /translation="KILYLFFVIGIVCVLTAGVQGLAADIELYLQAAENNPTDPVAQY
                     NLATVYYDQQRYDEALLSYQKAVALKPDFADAWFKLAAVYMQRKDSDKEIAAYQKAIQ
                     YKPNFAEAHYGLGLCYYLKKDYSVAVESFKTAIGIKGNQAEMYYYLGKAHTQQGQASE
                     AVAALLKATELNPNDANVWYQLGLSYGVLQDYAKEAESYQKSIALKPDAPNAYYNLGL
                     AYQGLGKFAESIDAFKRALVISPKHEEAMISSGLANVRLQRYSEALLSFQRVIEINAN
                     NAQAHFYQGYVLIKMGKNHEAYLAYQAAVKADPKFTKAYFQLGLLCFDLKKYDEEIEN
                     YKKVIELDPAFPNAHYNLGLAYLGSDKKELALAQCDELKKLDAPAANRLFGLINRK"
     gene            1391..1464
                     /locus_tag="VJC18_07775"
     tRNA            1391..1464
                     /locus_tag="VJC18_07775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1425..1427,aa:Arg,seq:acg)
     gene            complement(1521..1985)
                     /locus_tag="VJC18_07780"
     CDS             complement(1521..1985)
                     /locus_tag="VJC18_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45319.1"
                     /translation="MEPVKDKIERGLYEFQGLENLSYWTNILELQFENLVLNNLHDII
                     KALGIDFNSIVGAGPYYQNTTLAQQGCQIDLLVQTKFSTLYLCEMKFKKTIGTEVIIE
                     MNKKIAALKRPKHMSVRPVLIYEGGVENELRQAEFFDKMLCVGDLFRKPEKT"
     gene            complement(2103..3215)
                     /locus_tag="VJC18_07785"
     CDS             complement(2103..3215)
                     /locus_tag="VJC18_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD45320.1"
                     /translation="MPHTTAPAFKPKDPYQILGTGGENKQELDPQDTVVINGQIIYWP
                     GASDCFEDLQEGLQLGLNSFTCHGQVAQLSNDKLVSPLFSKKEQLTAYRQLLAKIADK
                     PVFESDRMLQDVGVLFSLLDRGFNKEARQLADQIAIRDQQMEPDDFTTALVNFDLAIA
                     YDRMNNPKQAQEHFVRAQTATSELIFDDYFFFLTSKMWQTLMTVRLPADFRRETLSGL
                     EWILPHIDNPTSRAAFLSRLGQFHPDKEQALNYFRQSFELLQNSGLMANRRSFLELLD
                     AMANASADIENETFEVSQSLLGIFFPLDGGDQETENLKYYFVQELHSAPLPPESKALL
                     YKRILDYENFMTHDLPGQNTSTRVADYVRGFMEEGD"
     gene            complement(3283..>3912)
                     /locus_tag="VJC18_07790"
     CDS             complement(3283..>3912)
                     /locus_tag="VJC18_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLD45321.1"
                     /translation="LHHLVQFFSKEQKPILVGHSWGAVLALLYAAKHKTSAHGIVVIG
                     TGPLDISVASKMNINIGRRLEKKDKQKITALKKQIVVEKDEKNKALLDLAFLKLVLPH
                     YNVSHRSLSCLQLGLRNTKTVDKCSADYTEKLTKGSLVRCLKKISLPVTGIYGYCDPI
                     PVPLNTSLLKKNIPQYTDIIIKNAGHFPWLEPKARQVFLRKLKLVLRHM"
BASE COUNT         1007 a          865 c          972 g         1068 t
ORIGIN      
        1 cgaaaatatt gtatttgttt tttgtgatcg ggatagtgtg tgtcttgaca gccggtgtgc
       61 agggattggc cgcagacatt gagctttatc tgcaagcggc cgagaacaat cctactgatc
      121 cggttgctca gtacaattta gccactgtct attatgatca acaacgctac gatgaggccc
      181 tgttgtcata ccaaaaggcc gttgcgctca aacccgattt tgctgatgcc tggttcaaat
      241 tggccgccgt gtacatgcag cgcaaagaca gcgacaagga aatagcggcg taccaaaagg
      301 cgatccagta taaacccaat tttgccgagg cccactatgg attggggctg tgttattatc
      361 ttaaaaagga ctattccgtt gctgttgagt cttttaaaac agcgattggc attaagggca
      421 atcaggccga aatgtattat tatttgggca aggcccatac ccagcagggt caggccagtg
      481 aggctgttgc ggcgttgctc aaagccactg agctcaatcc caacgatgcc aatgtctggt
      541 atcaattggg gctgtcttac ggggtcctgc aagactatgc caaagaggcg gagtcttatc
      601 aaaaatcgat tgccctcaaa cccgatgccc cgaacgccta ttacaacctc ggtctggctt
      661 atcagggtct gggcaagttt gccgagagca tcgacgcctt caagagggcg ctcgtaattt
      721 caccaaaaca cgaagaggcc atgatcagct caggactggc caatgtccgt ttgcagaggt
      781 attccgaagc gttgttgtcg tttcaaaggg tcatcgaaat caatgcgaac aatgcccaag
      841 cccatttcta ccagggttac gtgttgatca aaatggggaa gaaccacgaa gcttatttgg
      901 cttatcaggc ggcggtcaag gcggatccca agtttaccaa ggcctatttt caactggggc
      961 ttttgtgttt tgatttgaaa aaatacgatg aagagatcga aaattacaaa aaggtgattg
     1021 agttggaccc ggcctttccc aatgcccact acaacttggg cctggcatat ttggggtcgg
     1081 acaaaaaaga attggccctg gctcaatgtg atgagctcaa aaaacttgat gccccggcgg
     1141 cgaacaggct ctttgggctg attaacagga aatgatggag tgggggttgc ttttttattg
     1201 ccgaagtgtt tataaggcgc gggctctaca gaccaaggac taacccttcg acttcgctca
     1261 gggcagaaga tcaaagacca aagtcgggga aattaagatt gagaacgttc aggagtcaat
     1321 cggaacccaa agccgtcagg ctctttgtca caaacagaac aggtctttgc aatagagatg
     1381 aacgatcaac gcacccgtag ctcagctgga tagagcaact ggctacgaac cagtaggtca
     1441 tccgttcgaa tcgggtcggg tgcgctttca aattcaggtg atgtttcgag gcctggggcg
     1501 aacgccgacc gaagagggat tcaggttttt tctggctttc ggaacaaatc accgacacaa
     1561 agcattttgt cgaaaaattc ggcttgcctc aattcatttt cgaccccgcc ttcataaatc
     1621 aaaacaggcc tgaccgacat gtgttttggg cgcttgagcg ccgcaatttt cttattcatt
     1681 tctatgatca cctcagtccc aatcgtcttt ttgaatttca tttcgcacaa ataaagcgtg
     1741 ctgaattttg tttgaaccag aagatcgatc tgacaaccct gctgggccag agtggtgttt
     1801 tgataatagg ggccggcacc cacaatgctg ttaaaatcaa ttcccagcgc tttgatgatg
     1861 tcatgcaaat tattcaggac aaggttctca aactgaagct caagaatgtt tgtccagtac
     1921 gacaaatttt ccagcccctg aaattcatag agtccccgtt cgatcttgtc ttttactggc
     1981 tccacatatt ttaaataaaa tcgcaggtaa ttgtctttga tccggtatgt gctgagtctt
     2041 gacctctttc catcaagtct gccaaatgaa ttttcatgat ttaaggtgcc ctctaaccca
     2101 ggctaatccc cctcttccat aaaaccgcgc acatagtctg cgacgcgggt ggatgtgttt
     2161 tggcccggca ggtcgtgtgt catgaagttt tcgtagtcca gaattcgttt gtacagcagg
     2221 gccttggatt ctgggggtag tggggcggag tgcagttcct gcacaaaata atatttgaga
     2281 ttttctgttt cctggtcacc accatccagg gggaagaaaa tgcccaaaag ggattgggag
     2341 acttcaaagg tttcattttc aatgtctgct gaggcattgg ccatagcgtc gagcagttcc
     2401 aaaaatgatc ttctgtttgc catgaggcct gagttttgca acagctcaaa agattgtcta
     2461 aaataattca gggcctgttc tttgtcgggg tgaaactggc ccagtcgact taaaaaagcg
     2521 gcacgacttg ttgggttgtc aatgtggggc aggatccatt ccaaaccaga gagtgtttcg
     2581 cgcctgaagt cggcgggaag acgaacagtc atcaaggttt gccacatttt tgatgtgagg
     2641 aaaaaaaagt aatcatcaaa aatcaattcg ctcgtggcgg tctgtgccct gacaaaatgc
     2701 tcctgtgctt gtttggggtt attcatgcgg tcataggcga tggcgagatc aaaattgacc
     2761 aaggccgtgg tgaaatcgtc tggttccatt tgctgatccc tgatggcaat ttggtccgcc
     2821 agctgacggg cttctttatt aaatcctctg tcgagcagag aaaaaagtac ccccacatcc
     2881 tgcaacatcc gatcggactc aaaaacaggt ttgtcagcga tttttgccaa aagctgtcgg
     2941 taagcggtca attgttcttt tttgctaaac agaggggaaa ccagtttgtc gtttgaaagt
     3001 tgtgcgacct gtccatgaca tgtaaaagaa ttcagtccca gttgcaaccc ttcctgcagg
     3061 tcctcaaagc aatcggaggc ccccggccaa taaatgattt gtccgttgat gacgacggtg
     3121 tcttgcgggt caagctcttg cttgttttct ccccctgtgc caaggatttg ataagggtct
     3181 tttggtttga aggcaggtgc tgttgtgtgg ggcatgaaaa cctctaagtt accagcttca
     3241 tcggtcaggg acaccaaaag ttgctctaaa atgacaaatt aatcacatgt gccgcagaac
     3301 cagtttgagt tttcttaaaa acacctgacg ggccttgggt tccaaccacg ggaaatggcc
     3361 ggcatttttg atgatgatgt cggtgtattg tgggatattt tttttgagta gactcgtatt
     3421 gaggggaact ggaatgggat cacaatagcc ataaatcccc gtgacaggca gagatatttt
     3481 tttaagacac ctgaccagcg atccctttgt tagtttttct gtgtaatcgg cagaacattt
     3541 gtcgacggtt ttggtatttc tgagtcccaa ttgaagacac gaaagggagc gatgggaaac
     3601 attgtaatgt ggcagaacaa gtttgagaaa ggccaagtct aaaagggcct tgtttttttc
     3661 gtctttctcc acgacgattt gctttttcag ggcagttatt ttttgtttgt cttttttttc
     3721 cagccgccga ccaatgttga tgttcatttt actagccaca ctgatgtcca gaggaccagt
     3781 gccaataacg acaatgccgt gtgccgaagt tttgtgtttg gccgcgtata acaaggccaa
     3841 caccgctccc caggagtgcc cgaccaaaat gggtttttgt tctttggaaa aaaattgcac
     3901 tagatgatgt ag
//
LOCUS       DATDUN010000556         1286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_29785, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000556 DATDUN010000000
VERSION     DATDUN010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29785"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VJC18_07795"
     CDS             complement(<1..188)
                     /locus_tag="VJC18_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45322.1"
                     /translation="MTITQPIQLPLFPGLAGTGTAFFLPGQDKAFVAPHAFGHHLNDS
                     YDTLTTSGRLLLSPSREA"
     gene            complement(185..817)
                     /locus_tag="VJC18_07800"
     CDS             complement(185..817)
                     /locus_tag="VJC18_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45323.1"
                     /translation="MGRIDETNLSPHEPVFFFDDEGTAFLFEGNSVPEQHWEPCDAFD
                     DQVISIEFERLTKKEEFPKYSWGDCEKSTIINAQTLHPLLADYHPTYTYVWGDRFGTG
                     HAWLETQVCGASYLTDLVGGSEAGGLFGYGLNYFTPINNMQTSQDIPGYFMVEHQHEL
                     STNNPLNLDSSAYAKKQAEARDTLPRSVLENLVRSGARFLERVFKRNKPT"
     gene            complement(909..>1286)
                     /locus_tag="VJC18_07805"
     CDS             complement(909..>1286)
                     /locus_tag="VJC18_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45324.1"
                     /translation="HLIGKPQDSAPKLGWFKEMADTLRQSDHMRAGSAQHKLTNGLYL
                     KLARDICIKLGITDSRQGRVIYLLLWARLESLIKKCSTELFEQIIRDDHEGRHGEIIT
                     LLLDELHVERDEGIASYLGHAHK"
BASE COUNT          310 a          312 c          318 g          346 t
ORIGIN      
        1 cacgcctcac gggaaggcga tagcaacagt ctgccagatg tggtcagggt atcgtacgaa
       61 tcgttgagat ggtggccaaa ggcgtgcggc gccacgaatg ctttgtcctg acccggcaaa
      121 aaaaaagcag tgcctgtgcc agccaagccg ggaaacaagg gcagttgtat gggttgggtg
      181 attgtcatgt tggcttattt cttttaaaca cccgctccaa aaaccgcgcg cccgatctca
      241 ccagattttc cagtacagag cgagggagtg tgtctctggc ctctgcctgt ttttttgcat
      301 aagctgatga gtccaaattg agtgggttgt ttgtggacag ttcatgttgg tgttccacca
      361 taaaataacc ggggatatct tgactggtct gcatgttgtt gatcggtgtg aaatagttaa
      421 gaccgtaacc aaacaagcct ccagcctctg agccgcccac cagatcggtc agatacgatg
      481 ccccgcacac ttgtgtttcc agccaggcgt ggccggttcc aaaccgatct ccccaaacat
      541 aggtgtaggt ggggtgatag tctgcgagca ggggatgcag agtttgagca ttgattatcg
      601 tgcttttttc acaatcaccc caactgtatt ttggaaattc ttcttttttt gtgagcctct
      661 caaattcgat gctgataact tgatcatcaa acgcgtcaca aggttcccag tgttgctctg
      721 gaacggaatt tccctcaaag agaaaggcgg ttccttcatc gtcaaagaaa aaaacaggtt
      781 catgaggaga aagatttgtt tcgtcgatgc gtcccataac gtggcgctac ttataatcct
      841 atcgtcgacc aaaaaaaaga tttgttgcct tcctgccgca ctatccgtgt cctgtgggac
      901 acaagttgtt atttgtgtgc gtgccctaaa taactggcta tgccttcgtc ccgctcaaca
      961 tgcaactcat ccaagagtaa agtgatgatt tctccgtgtc gaccttcgtg gtcatcacgg
     1021 atgatctgtt cgaacagctc ggttgaacat tttttgatga ggctctccag acgggcccag
     1081 agcaacaggt agataaccct tccctgccgg gaatccgtga tgcccagttt aatgcaaatg
     1141 tcgcgggcca gtttcagata taagccatta gtcaatttgt gttgagcact accggcccgc
     1201 atgtggtctg attgtctcaa ggtgtccgcc atctctttaa accatcctaa cttgggagca
     1261 gaatcctgtg gcttaccaat caaatg
//
LOCUS       DATDUN010000557         1590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_29867, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000557 DATDUN010000000
VERSION     DATDUN010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29867"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VJC18_07810"
     CDS             complement(<1..1240)
                     /locus_tag="VJC18_07810"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLD45325.1"
                     /translation="MSYTLLCIDDDPDFLNNINLALSKHYEVILCQNFRTARLILDEK
                     DIDLVLLDIHLDQEDGIEVLKSIKSLYPQIEVMMLSGDRHARQIVESMKLGACDYLTK
                     DTNTSEIMSYIDRILGHKHTRDRLSAMISDVKAPAQQQQIMGTSQEISRLLSQAGRAK
                     GVGVNVLIQGETGTGKGALARYLHDLEGTNQRPFITLHCAETPVNSLESELFGHEKGS
                     SVGAHQRKLGKIELADGGDLFLAQVDQLSEELQLKLLQVLKTNEVFRLGAVKPLKVSF
                     RLIVSTSKDLPILVRQKQFRDDLYQRCCTLRLNVPALRERISDLDALTDYFLEKCNAA
                     HGDANRKTLHPEVREALKNYEWPGNVKQLESLMQSLVTVTEQTQIGINDLPLGVARAT
                     FLKDQPTLTMSKIGGALYRMN"
BASE COUNT          395 a          371 c          386 g          438 t
ORIGIN      
        1 aattcatgcg gtacaaggcc cccccaattt tggacatggt cagcgtgggc tgatctttca
       61 gaaatgttgc ccgcgccaca cccaaaggca gatcgttgat cccaatctga gtttgctctg
      121 tcactgtgac cagactttgc atcaggctct caagctgttt gacgttaccc ggccattcat
      181 agtttttgag ggcttcccga acctcggggt gcaacgtctt tcggtttgcg tcaccgtgtg
      241 ccgcgttgca tttctccaaa aaataatctg tcaaagcatc caggtcactg atccgctccc
      301 tgagtgctgg gacattcaat ctgagagtac aacagcgttg atacagatca tccctgaact
      361 gtttttgcct gaccaggata ggcaggtctt tgctggtcga gacgatcaat ctgaagctca
      421 ctttgagggg cttgaccgcg cccagcctga agacctcgtt tgttttcaga acctgcagga
      481 gttttagttg cagttcctca ctgagctgat caacttgcgc cagaaaaaga tcgccaccgt
      541 cggcgagttc aatttttccc aatttgcgct gatgggcacc cactgaagaa cctttttcat
      601 ggccaaatag ttcggattcc agggaattta cgggtgtttc tgcacagtgc agtgtgatga
      661 aagggcgctg gtttgtacct tccagatcgt gcagataacg ggccaatgcc cccttgcctg
      721 tgccggtttc accctgaatc agaacgttca cccccactcc ctttgcgcgt ccagcctgag
      781 aaagcagtct tgatatttcc tgtgatgtgc ccatgatttg ttgttgttgg gcgggtgcct
      841 tgacatcgct gatcatggca gacaaacgat cccgggtgtg cttgtgcccc agaatcctgt
      901 cgatgtaaga catgatctcg gaagtatttg tgtctttggt caggtaatca caggccccca
      961 gcttcatgct ctcgacgatc tgtcgggcat gtcggtcgcc tgacagcatc ataacttcaa
     1021 tctgtgggta aagggatttg atggatttca ggacctcaat tccatcttcc tgatccagat
     1081 gaatatcgag cagaaccagg tcaatgtctt tttcatcgag aatgagtctg gccgtcctga
     1141 aattctggca aaggatcacc tcataatgtt ttgacagggc aagattgatg ttgtttaaaa
     1201 aatcgggatc gtcgtctatg cagagcaggg tatatgacat gaaaccgcct gttaaaatct
     1261 tatatgataa aaaaacaaac agctatgcgc cttgcgacat tttgtagcgg tctaacgatt
     1321 ataaagagga gtgtcaaata aatattacta tatgtaaaaa tattttagat atcacggaaa
     1381 aatgtatgat aacctcgatt tttctggtga atcgaaaaac ttgattaaaa agggctttat
     1441 caaaatcttt cagatatgtt agcgtgcacg cgctgtgggg taaggttacg atggaccaaa
     1501 ggtcgtcttc gctgaaggat tcaactacgg cataccaaga gggatcacta tgaaaacaca
     1561 actgatcgtt atcggggcgg ggcctggcgg
//
LOCUS       DATDUN010000558         1220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_360323, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000558 DATDUN010000000
VERSION     DATDUN010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360323"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VJC18_07815"
     CDS             complement(<1..476)
                     /locus_tag="VJC18_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45326.1"
                     /translation="MPVFLYSGPASEQKRDRLFSLVNQHLTGQVPGIVVVPDQSYVNS
                     HKTRILLRQKERALIGQTVMTWDDLLLGWIKHNLPRVHLADQTFCHYLLFLLIEKNYP
                     ELIRDRHSAHATVLELYRFFQGLKSCGVGPEEVSQSLKNRLEQKELWALFTDYQAQ"
     gene            complement(549..>1220)
                     /locus_tag="VJC18_07820"
     CDS             complement(549..>1220)
                     /locus_tag="VJC18_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HLD45327.1"
                     /translation="GEGEGEGEGDADADADAEGDADADADGDAAAAAAADGDGDGIAD
                     TTDNCTSVSNVDQKDTDADRMGDACDPDDDNDGILDDGNYTVIIGDSYCEPDDTGNCD
                     DNCITVSNVDQADFDEDHIGDACDLDSDNDGLCDHDTLCTDIDNSERLVDLWYFVSQD
                     ATKSPPSVEGGYSYRFIEGKGAGAIIVVQGLTDRTLAEGTVGRCPGSGTTAKVGICIL
                     DFSIK"
BASE COUNT          339 a          326 c          257 g          298 t
ORIGIN      
        1 aactgggcct gataatccgt aaagagggcc caaagctctt tttgttcgag gcgattcttt
       61 aacgactggc tgacctcttc tgggccgacc ccacaggact tgagtccctg gaagaaccgg
      121 tacaattcca aaaccgtcgc gtgagcgcta tgacgatcac gaatgagttc ggggtaattc
      181 ttttcgatga gtaaaaaaag gaggtaatgg caaaaggtct gatcggccaa atggacgcgg
      241 ggcaggttat gtttgatcca acccagcagg agatcgtccc aggtcatgac cgtctggcca
      301 atgagcgcgc gttctttctg tctcaacaga atccgggttt tgtgggaatt gacatagctc
      361 tggtctggaa ccacgacgat gcccggcacc tgaccggtga ggtgctggtt caccagggaa
      421 aaaagtcggt cgcgcttttg ctcactcgcg gggcctgaat acaaaaagac gggcatggga
      481 taggcttaga tgattagaca aaatagctca acattttttg aaaggattga gtctattcat
      541 cagttgtatt attttataga gaaatctaga atgcaaatac ccactttagc cgttgtaccc
      601 gaacccggac aacgacctac agtaccttct gccaacgtgc ggtctgttaa cccttgcaca
      661 acaataatag caccagcacc ctttccctca ataaatcgat atgaatatcc gccctcaact
      721 gaaggaggtg atttagtagc atcctgactt acgaaatacc aaagatcaac aagtcgctcc
      781 gaattatcaa tatccgtgca tagcgtgtca tgatcacaca acccatcgtt atcggaatct
      841 aagtcacaag catcaccaat atgatcctca tcaaaatcgg cttgatcaac attgctaacg
      901 gttatgcagt tgtcatcaca attaccagtg tcatctggtt cacaataact atcaccaata
      961 atgacagtat aattaccgtc atctaaaatg ccatcgttgt catcatcagg atcacacgca
     1021 tcacccatcc tatcggcatc cgtatccttt tgatccacat tggaaacgct cgtgcaatta
     1081 tccgtcgtat cggcaatccc atcaccatca ccatcggcag cggcagcggc agcggcatca
     1141 ccatcggcat cagcatcagc atcaccttcg gcatcagcat cagcatcagc atcaccttca
     1201 ccttcacctt caccttcccc
//
LOCUS       DATDUN010000559         1151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_315903, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000559 DATDUN010000000
VERSION     DATDUN010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315903"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VJC18_07825"
     CDS             complement(<1..283)
                     /locus_tag="VJC18_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLD45328.1"
                     /translation="MTSKGSSQTFDTIIIGAGIAGLTAAYYLKQKNQKILLFEKQNTA
                     GGNIKTLRLGDFLCETGPHSFMGSAEAVWKLVHDLNLSSQVLKASPSAQN"
     gene            424..756
                     /locus_tag="VJC18_07830"
     CDS             424..756
                     /locus_tag="VJC18_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45329.1"
                     /translation="MVFWRRPLIKFLSSLKLGVFLLLALIMLAAWGTFIESRMGALYA
                     KRFLYLSPGFVFLEALLFLNVLMAMLVRLPPRKKLCGFYLAHAGVLLLLAGAGMTGIW
                     GLDGSLTL"
     gene            802..>1151
                     /locus_tag="VJC18_07835"
     CDS             802..>1151
                     /locus_tag="VJC18_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45330.1"
                     /translation="MFRVMEDLQAQPREGKVSLPSTVREKPLGPILVDTGQTRIVLRR
                     YLPFAKEVIEQGSVLRWESVLPERGTVDVFPAVELGVTAVAENGQVEEQVVWVSARDI
                     TAQHIFHTAFLQAL"
BASE COUNT          240 a          252 c          357 g          302 t
ORIGIN      
        1 ggttttgcgc cgacggtgag gccttgagca cctgcgacga cagattgaga tcgtgcacga
       61 gcttccagac ggcctcggct gaacccataa acgaatgagg cccggtttcg cacaaaaaat
      121 cgccaagcct gagcgttttg atgttgccac cagccgtgtt ttgtttttcg aacaacaata
      181 ttttttgatt tttttgtttg agataatatg ccgccgtgag cccggcaata ccggcgccga
      241 tgatgatggt gtcgaacgtc tgggatgagc ctttggatgt cacgggcggg atgagaacaa
      301 atgcggaggt gggcagtcaa tggaataatg aaataatgaa gaaatgaagc gatgaaacaa
      361 tgaaaaaatg atgggatgtt ggtgttgcgt tgacaggcgt gcgacgggga gattaataag
      421 ccagtggtgt tttggcgtcg ccccctcatc aagttcctca gctccctcaa gttgggtgtg
      481 tttttgcttt tggcattgat catgctcgcg gcgtggggga cgttcattga aagccggatg
      541 ggggcgttgt acgcgaagcg ttttttgtac ctctcacccg gttttgtttt tttggaagcc
      601 ctgttgtttt tgaacgtgtt gatggcgatg ttggtgagac tgcctccccg gaaaaaactg
      661 tgtggctttt atctggcaca tgcgggtgtt ttgctcctgc ttgcgggcgc cgggatgacc
      721 ggtatttggg gcttggatgg tagcctgact ttgtaagcgc acgaacccag tcaacaggtc
      781 acgacgaacg attgggacct gatgtttcgt gtgatggagg acttgcaagc acaaccccgt
      841 gagggcaagg tttctctgcc gtcaacggtg agggaaaaac ccctggggcc catcttggtc
      901 gataccgggc agacaagaat tgtcctgagg cggtacttgc cttttgccaa agaggttatt
      961 gaacaaggct ctgtgttgcg ctgggagtca gtcttgcctg agcgcggcac agtcgatgtt
     1021 ttcccagccg tggaactggg cgtgacagcc gtcgcggaga acgggcaggt tgaggaacag
     1081 gttgtttggg tgagcgcgcg cgacatcacg gcccagcata tttttcacac cgcttttttg
     1141 caggccttgt t
//
LOCUS       DATDUN010000560         1276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_278625, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000560 DATDUN010000000
VERSION     DATDUN010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278625"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VJC18_07840"
     CDS             complement(<1..622)
                     /locus_tag="VJC18_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLD45331.1"
                     /translation="MGEIFALATAVIWAHGSVFFTSASRRAGSFFVNRTRALFGSLIL
                     LVLHFVMYGFLFPQLTLNQFALLLLSSATGLVIGDSFLLQAYVDIGPRFTLLIGNLTP
                     FFTAILAALILGEHLSLLAWTGMLVTITGTVWVMFDARFFNKNLLQTRPLRGVIFAML
                     FALCQGFGFVIVKPVIHGDQAILPLSATLIRLCMATVFWMIITLFVR"
     gene            complement(729..>1276)
                     /locus_tag="VJC18_07845"
     CDS             complement(729..>1276)
                     /locus_tag="VJC18_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HLD45332.1"
                     /translation="PTELQDQITSYVSATMSTEARATFESAMAENHLLTLAVEIAKAK
                     QQQADPAASVCIAQKFIEMDASRDLVASAETAGSEVDLADSLGHVSALRLISQQWVRC
                     SDPLSERLPDFLGGLLEDDEMAQFEKHLLSCPTCYEEACWNLEFVANHSELPGAQEAG
                     EALQALKNAWAERHPAEDLVV"
BASE COUNT          359 a          326 c          314 g          277 t
ORIGIN      
        1 ccctaacgaa aagcgtgatg atcatccaaa acactgtggc catacacaac cggatcaagg
       61 tcgcactcaa cgggagaatg gcctggtcac cgtggatcac gggtttgact atcacaaaac
      121 caaacccctg acacaaggca aagagcatgg caaaaatcac ccctcgcaat gggcgtgtct
      181 gtagaagatt tttattgaaa aatcttgcgt caaacatgac ccaaacagtg cccgtaatgg
      241 tgaccagcat ccctgtccaa gctagcaggc tcaagtgctc gcccagaatc agggcggcca
      301 ggatcgccgt gaaaaagggg gtcaggttgc cgatgagcag agtgaaacgc gggccaatat
      361 ccacgtaggc ttgcagcaaa aaagaatctc cgatcacgag tcctgtcgca ctgctcaaaa
      421 gcagtagggc gaattgattc aaggtcaact gcggaaagag aaatccatac atcacaaagt
      481 gcaggaccag aaggattaga ctgccgaaca aggccctggt gcgattgaca aaaaaggacc
      541 cggccctgcg ggaggccgat gtaaaaaaaa ccgagccatg tgcccagatg acggctgtgg
      601 ccaatgcgaa gatttcaccc aaaaaaggca tgggggttga tagtggtttt tagggcggga
      661 gtaaatgact gttttggttt gtgaaaaaaa cggaaccagc aaaggttatc atacaatgac
      721 tgtgattatc atactactaa gtcctctgct ggatgacgct cagcccatgc gtttttgagt
      781 gcttgcaatg cttctccagc ctcttgtgct cccggtaatt cggaatgatt tgcaacgaac
      841 tccaagttcc aacacgcttc ttcataacat gtggggcaac tcagcaaatg cttttcaaat
      901 tgcgccattt cgtcgtcttc gagtagcccg cccaaaaagt ccggtagtcg ctcactaagg
      961 gggtctgaac accttaccca ttgctgagaa atcaacctca aagcactcac atgtccaaga
     1021 gagtctgcca aatcgacctc ggaaccggca gtctccgctg aggctaccag atcacgtgat
     1081 gcatccatct caataaactt ctgcgctata caaacactcg cggccggatc tgcttgttgc
     1141 tgctttgctt tggcaatctc gacagcgagt gtgagcaagt ggttttctgc cattgcactt
     1201 tcaaaagtgg cccgcgcctc cgtgctcatc gtagcactga cataagaggt gatctgatcc
     1261 tgcaattcag taggga
//
LOCUS       DATDUN010000561         3873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_360432, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000561 DATDUN010000000
VERSION     DATDUN010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360432"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VJC18_07850"
     CDS             <1..791
                     /locus_tag="VJC18_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD45333.1"
                     /translation="RAGFNTSGFIHRVIKGNRNLTLESTRKIASLIPLRGRPLKYFET
                     LVQFNQAKTQKDREQNFEALKEFKEFTEERLLRKDQMVLLLKWYYPVILEMVRWHDFK
                     PDPSWISQRIRPKLYMEQVKNALDVLQRLELIAVDSEGRWYQTHSRLSTPLQGMGKEA
                     FIFHSNAMILSQKNLNLPKEDRFYTSKLISASPAHFDALQQLISNFHKEVDRMLQRKP
                     DRQFLVEHGLDEARLDQPDFTKTTEVTQLNIQLFKVAKQRGYYE"
     gene            784..3021
                     /locus_tag="VJC18_07855"
     CDS             784..3021
                     /locus_tag="VJC18_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45334.1"
                     /translation="MNNLVILRGFSNGLLVRFCCTLVFCSFCFLNSSCQGLANKASSP
                     DDATIEITPQQDPGVGSEGDSSTSTITIGNPGWDIDTSGTVYLSLPQSDDNLKQLVDD
                     VMNSVFAVDVSDTTEASQTTLPAIKQFQLGPNGELVILFEEPVIWDAENCSLFYTRNG
                     EGHCLETSTVSLQDNIQFDAAGNIFYFGKTNPDDTNTLVRWDANSKQKSSLINPYLTL
                     HQWSVHTNGMAYFTGATSGATFFRTLNPADNTVTNLLPDAATVTAFNFISATHLIVQG
                     DNIYHNNTYQSGNFLYDLVAQVTTERLDTDGGDLMNLQIDDQNRVYVLKDHAIQMIYP
                     GVPFTVETNLGEINLFKIYHDTLYAVGKSGLAQQLVAVDLTEAEGLPRVLLNNQEIYH
                     LEYDNGSLYFDHLNFGDNSYSLSKFDLLTETLTKIESINDQLGQIQSLASAQNYSVQQ
                     RDFAETQKYQVSVSYSDEPIHPALATSSEPAISPLTASVFLQTTLCAATNEQAVFEDL
                     VIYISCDGAIAAHLDASPEGSAQTLLTSDYVDYVDVFVISEARNLVGIHTKRVVSASE
                     YPSQSLMIYSYAIDENTASVGLEAHSIISLDETGAFEFASANEGDAVFVFDKTTKQLL
                     LYTNDASQDSALGVGLAQYAGTATLIGEKNNAVFVAVTNQWDTDSEETKILLIKKSNA
                     ELELVQSATIDALALNYEIVSSLEDHYTIKATTSTGVYQISFDDGPFVQGMSVNEPRI
                     LSNNN"
     gene            complement(2973..3494)
                     /locus_tag="VJC18_07860"
     CDS             complement(2973..3494)
                     /locus_tag="VJC18_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HLD45335.1"
                     /translation="MKKIFLFAVLFLLLSQSVFATVELKPITHTEGKYSISLPTNWEY
                     ALNYRGASLVAHRISSGPLDLFAENISVISEALPLEITAPQYYERNLVTMKEVLKELR
                     IVSLENVVVNSLSAVKLVSAYRVGNNKVQAATLFATVGKQAYVVTLISLEDRYSESQL
                     LFDKILGSFTLMP"
     gene            complement(3688..>3873)
                     /locus_tag="VJC18_07865"
     CDS             complement(3688..>3873)
                     /locus_tag="VJC18_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45336.1"
                     /translation="SPSLSPQGGIPFAGQKGGAVPSCCLLRNKISKRKAFFTHFYVAH
                     PSGLGYLSVNDACGLSG"
BASE COUNT         1034 a         1017 c          859 g          963 t
ORIGIN      
        1 agagggccgg gtttaatacc tcgggcttca ttcaccgtgt gatcaaagga aatcgaaatt
       61 tgactctgga gagcacccgc aaaatcgcca gcctcattcc tctgcgagga cgccccctca
      121 aatattttga gacactggtc cagtttaatc aggcaaaaac acaaaaagac agagaacaaa
      181 attttgaagc cttaaaagag ttcaaagagt tcaccgaaga acgccttttg cgtaaagacc
      241 aaatggtttt acttttaaaa tggtactacc ccgtcatcct cgagatggtc agatggcacg
      301 atttcaaacc cgacccctct tggatctccc aacgtatccg acccaaacta tacatggagc
      361 aggtcaagaa cgcgttggat gttctccaaa ggcttgagct gatagcggtt gacagcgaag
      421 ggcgctggta tcagacacat tcccgtctga gcacaccatt acaaggaatg ggaaaggaag
      481 cctttatttt tcattccaac gcaatgatct tgagtcaaaa aaatttgaat ctgccaaaag
      541 aggatcggtt ctacacctca aaactcatca gtgcctcacc ggcccacttc gatgccctcc
      601 aacagctcat ttctaatttt cacaaggaag tggacagaat gctccagcgc aaaccggacc
      661 gccaattcct tgttgaacac ggcctagatg aggccaggct ggatcagccc gattttacca
      721 aaacgactga ggtgactcag ttaaatatcc aactcttcaa ggttgccaaa caaagaggtt
      781 attatgaata atttagtgat cctcagggga ttttcgaatg ggctgttagt ccgtttttgt
      841 tgcacgctcg ttttttgctc tttctgcttt ctgaattctt cctgtcaggg cttggcaaac
      901 aaagcctcat cacccgacga tgccaccatc gagatcacgc ctcagcagga cccgggggtg
      961 ggcagtgaag gcgattcctc tacaagcacc atcaccattg gcaaccctgg ctgggacata
     1021 gacacctcgg ggacagttta tctctccttg ccacaatccg atgacaacct taaacaactc
     1081 gttgacgatg tgatgaactc agtcttcgca gtagatgttt ctgatacaac agaggcctca
     1141 caaacaacac ttcctgccat taaacagttt caacttggcc ccaacggcga actcgtcatc
     1201 ctctttgagg aacctgtcat ttgggatgct gaaaactgct ctctattcta tacgcgaaat
     1261 ggtgaggggc attgccttga aacgagcacc gtttccttgc aggacaatat acaattcgat
     1321 gccgcaggaa acatttttta tttcggaaaa acaaatcccg acgataccaa cacccttgtt
     1381 cgctgggatg ccaactcaaa acaaaaatca tcgctcatca atccgtacct gaccctgcac
     1441 caatggtctg tccacaccaa tggcatggcg tattttacgg gcgcgaccag tggcgcgacc
     1501 tttttcagga ccctgaatcc ggctgacaac acggtcacaa acctgctccc cgatgccgcc
     1561 acagtcacgg cgttcaattt catttctgcc actcacctca ttgtgcaggg tgacaacatt
     1621 tatcacaaca acacgtatca atcgggaaat tttctttatg acctggtcgc tcaagtcacc
     1681 acagaaagac tcgataccga cggcggtgat ctgatgaacc tgcagattga tgatcaaaac
     1741 agggtgtacg ttctgaagga tcatgccatc caaatgattt atcccggcgt tcccttcaca
     1801 gtcgaaacaa atctgggaga aatcaacctg tttaaaatct accatgacac cttatacgca
     1861 gtcggcaaaa gcggtctggc acaacaattg gttgcagttg atctgactga ggcagaaggc
     1921 ttgccccgcg tcctcctcaa caatcaggag atttatcatt tggaatatga caatggctcc
     1981 ttgtattttg atcatttgaa ttttggcgac aattcttatt cgttgtccaa atttgatctg
     2041 ctcactgaaa ctctcacaaa aattgaatca atcaacgatc agttggggca aattcaatcc
     2101 ttggcatccg cgcaaaatta ttcggtccag caaagagact ttgccgagac acaaaaatat
     2161 caggtgagcg tttcttattc agacgagccc atccatccag ccctggccac aagcagtgag
     2221 ccagccatct ctccgttaac agcgagcgtg tttcttcaga ccacactgtg tgcggccaca
     2281 aacgaacagg ctgtttttga agaccttgtg atctacatct catgtgacgg cgcgattgcc
     2341 gcccatcttg acgcttcgcc cgaagggtca gcgcaaacac tgctcacatc ggattacgtt
     2401 gattatgtgg acgtgtttgt catttccgag gccaggaatt tggtgggcat ccacacaaaa
     2461 cgcgttgttt ccgcgtcgga atacccttcg caatcgctca tgatatattc gtacgccatc
     2521 gacgaaaaca ccgccagcgt tggcctcgag gctcactcca tcatttccct tgatgaaacc
     2581 ggcgcgtttg aattcgcctc ggcaaatgag ggtgatgctg tgttcgtgtt cgacaagacg
     2641 accaaacaac ttctgctcta tacaaatgac gcctctcagg acagcgctct cggcgtgggc
     2701 ctggcccagt acgcaggcac cgcaacgctc attggcgaaa agaacaatgc cgtctttgtt
     2761 gccgttacaa atcagtggga caccgattct gaggagacca aaatattgct gatcaaaaaa
     2821 tcaaatgctg agctggaact cgtgcaatcc gccacgattg acgcgctggc cctgaactac
     2881 gaaatcgtca gctcgcttga agatcactac acgatcaaag ccactaccag cacaggggtt
     2941 tatcaaattt cgtttgatga tggtcccttc gttcagggca tgagtgtaaa cgagccgagg
     3001 attttgtcga acaacaattg actctcagaa tatctgtctt cgagcgaaat gagggtcacc
     3061 acataggcct gcttccctac agtggcaaac agcgttgcgg cttgaacctt gttgttgcca
     3121 accctatacg cgctcaccag ttttacagcg gagagactgt tgacaacgac attttccaaa
     3181 gaaacaattc gaagctcctt gagaacctct ttcatggtca ccagatttct ttcgtaatac
     3241 tggggggcgg tgatttcgag gggaagtgct tcggaaatga cgctgatgtt ttcggcgaac
     3301 agatcgagag gcccgctgga aattctgtgc gcgaccaggc tggcccccct gtaattgagg
     3361 gcatactccc agttcgtggg caaactgatg gaatatttgc cctctgtgtg cgtgatgggc
     3421 ttgagttcca ccgtagcaaa aacagattga gacaataata aaaacaacac tgcgaacaaa
     3481 aatattttct tcatactcat tgctcctgac tttgtcgcga taggtaatga gccacgttct
     3541 atttgtcaat cacgatatcg ctctctatgt tgtgtcagtg aatgacgctg gcgttctttc
     3601 gcgggtcctg ctccgggtcg ctattttttg acgctctcca ctcgtaaact cgtttcgtaa
     3661 accgcgtcaa aataacagct ccccgtatca cccgctcaat ccacacgcgt cattcactga
     3721 cagataaccg agcccacttg ggtgtgcgac ataaaagtgg gtaaaaaagg cttttctctt
     3781 tgaaatctta ttgcgcaaca agcaacagga gggcaccgcc cctccttttt gtcccgcaaa
     3841 cgggatcccg ccttgcggtg ataaggaggg gct
//
LOCUS       DATDUN010000562         4558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_278690, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000562 DATDUN010000000
VERSION     DATDUN010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278690"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VJC18_07870"
     CDS             <1..646
                     /locus_tag="VJC18_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLD45337.1"
                     /translation="HTIITSHSLQEIRISDGKVRELVFENGEVRHADLFVSALPLHSF
                     VKAMPRNFLDSLPRSQGLCQTGHSAIISLNLFFDRPVMTDPLIGLARSDVHWFFNKTQ
                     PADRFSHIVGTISAAGSLLTNSKDELLAKALKDLKNIYPQMQKAQLVHALVNKDAFAT
                     VLGDATFNQARPAQRWFDNFYVIGDWTQTHLPPTLESAAQSAKNMYDELLPLSQ"
     gene            complement(836..1735)
                     /locus_tag="VJC18_07875"
     CDS             complement(836..1735)
                     /locus_tag="VJC18_07875"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLD45338.1"
                     /translation="MDYKWKVKKSERGMSLENYIYKQMGDWSHNQVKKAIDHKRAFIN
                     GKNVFVSKWNLKPNDVVLFVPSQADQPQAQVGRYRFVEVLFEDTYLLATNKPPFVEFE
                     AYAQVVQDYLKRKNTIKSYPYLGQMHRLDKETSGVMIYTKKKIANALADQFRAHDIQK
                     YYLSLVDGQVSGEEGQITKPLEKGEFEEGKKVRVARNSKDGLKAITRWRVLERYQKAT
                     LLSVEILTGRTHQIRVHLSDMGYPIVGDKLYGSGEGLSFKRQALHAERLHFTHPITKK
                     KMKITAPIPSDMAKLIDQLRLGT"
     gene            complement(1890..2579)
                     /gene="rsmI"
                     /locus_tag="VJC18_07880"
     CDS             complement(1890..2579)
                     /gene="rsmI"
                     /locus_tag="VJC18_07880"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HLD45339.1"
                     /translation="MSGILYVVATPIGNLKDISQRLRETLEAVAVILAEDTRVTLRLL
                     NHLGIKKRLFSYTDHASEQQEDKIISELESGVSFALVTDAGTPGISDPGHLLVERVLA
                     AGLGVIPVSGPSAVATLVSVFGKPHTAHHFWGFFPTKTKKQKELVASIDLLSGIHVFF
                     ESPFRILKTLEKYFLDKEHYHLVIGREMTKLYETFYRGTPQAVYEQLKAGVVKGEFCV
                     GVMAQGSRPKA"
     gene            3209..3769
                     /locus_tag="VJC18_07885"
     CDS             3209..3769
                     /locus_tag="VJC18_07885"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HLD45340.1"
                     /translation="MKLKQEHFARLSLKILSFLMQKKSLTMKMDEAAIKSTITTIFVQ
                     DALKIENITKKAQTVMEQHKAKVQTGEIDYQKMFPMIKKELFKKEGLPLSETNYSEDH
                     IKKLSLKIHDKLYLDNMVDYHDDEQSLMDIQHVMGEFFAIREKAVATARQKVSSLSKN
                     VAPGSNEWNVLFDKYLTEELKKWGFH"
     gene            3814..>4558
                     /locus_tag="VJC18_07890"
     CDS             3814..>4558
                     /locus_tag="VJC18_07890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HLD45341.1"
                     /translation="MPETSVIILRILLPFTVMLALIPLLVWLERKGSAYIQDRPGPNR
                     AAILGIRLGGLLHPLADVIKLAFKEDFIPSGANRFYYYLAPFLTFCVASVTFLVIPFG
                     SPITFGTQIIHLQAASLNIGILYILAISSLGVYGIMLAGLASHNKFALLGGLRASAQM
                     ISYELSLGLAIISLVMTTGSLDLQEMVQQQSPLLWRWNFIRDPLAFLIVIVASFAEIN
                     RIPFDLPEGESELVAGYHVEYSSLKFAMFF"
BASE COUNT         1148 a         1173 c          962 g         1275 t
ORIGIN      
        1 tcacacgatc atcacatccc actccctgca ggaaatcagg atcagcgatg ggaaggttcg
       61 ggaacttgtt tttgaaaatg gggaggtcag gcacgcggat ctttttgtca gcgcgcttcc
      121 gctccattcc ttcgtcaagg ccatgccccg gaactttctc gactccctgc cccggtcaca
      181 ggggctttgc caaacagggc actctgccat catttcgctc aatcttttct ttgaccggcc
      241 ggtcatgacc gatcccctca tcgggcttgc gcgctctgac gtgcattggt ttttcaacaa
      301 aacccaaccg gcagacaggt tctctcacat tgtggggacc atcagcgccg caggatcttt
      361 gctgaccaac tcgaaagatg aactgctggc caaggccctt aaggatctga aaaatatcta
      421 tccccaaatg cagaaggcgc aactggtaca cgccctcgtc aacaaagacg cgttcgccac
      481 cgtcttgggt gatgccacgt ttaatcaagc tcgtccggct cagagatggt tcgataattt
      541 ttacgtcatt ggcgattgga cacagaccca cctgccgccc actctcgaat cggccgcaca
      601 gagcgcgaag aacatgtatg atgaattact gcctttatca caatagccaa gaacatttct
      661 tagagcctag tcgaaaacct accgagagtc ccttttttgg gtcctgcccc ggctcgctgt
      721 tttttggcgc tctgggctcg caaactcgcc cagcggcctg ctggcctgtt cgcgcaaaaa
      781 cagctcaccg tgtcacccaa aaaagaccgc ttcgcggaag gttttcgaat gggctctaag
      841 tgcctaatct cagttgatcg atgagtttgg ccatgtccga tgggatcggg gcagtgattt
      901 tcatcttctt cttggtgatg ggatgcgtaa aatgaagtcg ttcggcatgc agggcctgtc
      961 tcttaaaaga tagcccctcg cccgacccat acaatttatc gcccacaatc gggtacccca
     1021 tatcgctcag gtgcacacga atctggtgag tacgcccggt gaggatttcc acactcaaca
     1081 gcgtggcctt ttgataccgt tccaaaacgc gccagcgagt gatcgccttg agtccgtctt
     1141 tgctgttacg ggccactcgc accttttttc cttcttcgaa ctcacccttc tccagaggtt
     1201 ttgtgatttg tccctcttcg ccggaaacct gtccatccac cagagacaga taatattttt
     1261 gaatgtcatg cgcgcggaat tggtccgcca aagcattagc gatctttttt ttggtgtaga
     1321 tcatcacgcc cgaggtttct ttgtccaaac gatgcatttg ccccaaatac ggataacttt
     1381 tgatcgtgtt ttttctcttt aaataatcct gcaccacctg cgcataggcc tcaaactcca
     1441 caaagggcgg tttgttcgta gccaacaggt aggtatcttc gaagagcacc tctacaaaac
     1501 ggtaacggcc aacttgggct tgcggttgat cggcctggga tgggacaaac aggaccacat
     1561 cgtttggttt gagattccat ttgctcacaa agacattttt gccattaatg aaggcccgtt
     1621 tgtgatcgat cgcttttttc acctgattgt gcgaccagtc tcccatctgt ttgtagatat
     1681 aattttcgag gctcatgccc cgttcacttt ttttgacttt ccatttgtag tccatatttg
     1741 cgtccttaaa cgtgttcacg tattcatgtg ttcaagtgtt tacgtgagtt ctaaaatccg
     1801 ctgacagctt cggctaacag ctaacaacca gaaactgacc gttggcagtc gactgtcggc
     1861 tatacacctt gtgccttaag ccttaagcct taagccttag gccttgagcc ttgagccatc
     1921 acccccacac aaaactcgcc cttcaccaca ccagccttga gttgttcgta aacggcttgc
     1981 ggtgtgcctc gataaaaagt ctcatacagt ttggtcatct ccctgccaat caccaggtga
     2041 taatgctcct tgtctaaaaa atatttttcc agggttttta ggatacgaaa cggcgattca
     2101 aagaaaacat gtatgccaga cagaagatca atagacgcaa ccaattcctt ctgtttcttg
     2161 gtcttggtgg ggaaaaagcc ccaaaaatga tgcgctgtgt gtggtttgcc aaaaacgctc
     2221 accaaagtcg ccaccgcgct ggggccagaa acaggaatca cgcccaagcc cgcagccaac
     2281 accctttcga ccaaaagatg accgggatcc gaaatgcccg gcgtgccagc atcggtcacc
     2341 aaggcaaaac tcacaccgga ctcaagctca gaaatgattt tgtcttcctg ctgttcagaa
     2401 gcgtggtcgg tataggaaaa caggcgtttt ttgatcccca gatgattcag caaccgcaac
     2461 gtgacccgtg tgtcctcagc caggatcaca gcaaccgcct cgagggtttc acgcaaccgc
     2521 tgggagatgt cctttaaatt gcctatgggc gtggccacca cgtacaaaat cccgctcatg
     2581 atgaaatcct cgggcaaaaa aaaagcccca aagcaaatga attaccctgg agctttttgg
     2641 ttctttacca aaaattgagt tactatcttt ttttcttcgc aacttttttc ttggcgactt
     2701 ttttcttggc aacttttttt acttttttct tagcaggcat cgtgcctcct ttattattgg
     2761 tgtgggttga taaagttaac ctaatttaac tttacatagc aattatattt gttttttttt
     2821 gattgtgtca aatatttttt atgccttttc aacaacttgt cgatggaaaa agttgttttg
     2881 actgcaaaag caaccttttt tgttcagcac gagaaaattc atcgctccca gcaaactctt
     2941 gacacataat tgtttgctgt ctcttttttt gatatttgtt ctcacaatcg acgcgtttca
     3001 ttttttttgg caaaaaaaat tctgaagtga tttttttttg tttttttttg ttttttttcg
     3061 caaaaaaata atttttggcg aaaaaaacat tgtgaattga tttctctgcc atcagtgaag
     3121 ggactgacca aatacatttc tcagcctttt tcaaatgaca accccttgtt tattacgtat
     3181 gctacgcccc taaccaaagg gctcccacat gaaacttaaa caagaacact ttgccagact
     3241 atctcttaaa atcttgtctt ttttgatgca aaaaaaatct ttgaccatga aaatggacga
     3301 agccgccatc aaaagcacca tcaccacaat ttttgtccag gatgccctca aaatcgagaa
     3361 catcaccaaa aaagcccaga cagtcatgga acagcacaag gccaaggtgc aaacgggaga
     3421 aatcgattat caaaaaatgt tccccatgat caaaaaagaa ctgttcaaaa aagaggggct
     3481 gccactctca gaaaccaatt actcggaaga tcacataaaa aaactgtccc tcaagatcca
     3541 cgacaaactt tatctggata atatggtcga ttaccacgat gatgagcagt cactgatgga
     3601 catccaacac gtcatggggg aattctttgc gatcagggaa aaggctgtgg caactgcccg
     3661 tcaaaaagtc agctcactga gcaaaaatgt cgctcccgga tcaaatgaat ggaacgtcct
     3721 gttcgataaa tacctcaccg aggaactcaa aaaatggggg tttcattaaa ttccgttgtc
     3781 ttgatccaca cgattggtta gaaaccgaac acgatgcccg aaacatctgt tatcattctt
     3841 agaattttac tccctttcac ggtcatgctg gcattgattc ccctgttggt ttggttggaa
     3901 cgcaaaggct ctgcctatat tcaagacaga cccggaccca accgcgccgc cattttggga
     3961 atacgactcg gtgggctact acaccctctg gctgatgtaa taaaattggc ctttaaagaa
     4021 gacttcatcc cctccggggc caacaggttc tattattatt tggcaccatt cctcaccttt
     4081 tgtgtcgcca gcgtgacttt tctggtcatt ccctttggct cacccatcac gttcggcaca
     4141 cagattattc atctgcaggc ggcttcgctt aacatcggca tcttatatat attggccatc
     4201 tccagcctgg gtgtctacgg gatcatgttg gccggcttgg cttctcataa taaattcgcc
     4261 ctgttaggag gtctcagggc aagtgcccag atgatcagtt acgaactcag tttgggactg
     4321 gccatcattt ctcttgtgat gaccaccggt tccttggact tgcaagagat ggtacaacaa
     4381 caatcgccac ttttgtggcg atggaatttc atcagggacc ccctggcttt tttgatcgtt
     4441 atcgtggcct cttttgccga aatcaacagg attccctttg atctgccaga aggcgaatca
     4501 gagttggttg ccgggtatca tgtggagtac tcctctctca aattcgccat gttcttca
//
LOCUS       DATDUN010000563         1683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_30092, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000563 DATDUN010000000
VERSION     DATDUN010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30092"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /gene="fusA"
                     /locus_tag="VJC18_07895"
     CDS             <1..312
                     /gene="fusA"
                     /locus_tag="VJC18_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090101.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLD45342.1"
                     /translation="FRAKPIVLEPIMKLEVTVPNEFQGAATGLINQRRSIVISTMNEH
                     GFTTVQAEAPLAAMFGFSTDIRSLTQGKGEFTMEFLKYAPIPKGLQEELVVDYQKKKN
                     S"
     gene            complement(370..778)
                     /gene="rnpB"
                     /locus_tag="VJC18_07900"
     ncRNA           complement(370..778)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VJC18_07900"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            956..>1683
                     /locus_tag="VJC18_07905"
     CDS             956..>1683
                     /locus_tag="VJC18_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45343.1"
                     /translation="MKKHNATIQTLVLALCLGLLLNCGQSPLDLDDTLDPNGTDDNEL
                     AGNTSVSTACAGLDLVDVTEDINDNTTWKGDTVYVIRAWNFYVNATLVIEPGAIIKFH
                     ATDGPEMVLGGAGTILAQGTEASPIIFTSYKDDTHGCDNNGDGTDSLPAAKDWGSVDT
                     NGLNGSIFDHCEFYYGGDTAYSATLALSAGSIATVINSTFAHNDGGDESGWYGAFDAS
                     HAEAGTVIQNNVFYDNVRPLSISVA"
BASE COUNT          421 a          467 c          412 g          383 t
ORIGIN      
        1 tttcgcgcaa agccgatcgt gctcgaaccc atcatgaaac tcgaggtcac ggttcccaat
       61 gaattccagg gcgcggccac aggactcatc aatcaaaggc gcagcatcgt catcagcacg
      121 atgaatgaac acggcttcac caccgtacag gccgaggccc ccttggcggc catgtttggt
      181 ttttcaacag acataagatc tttgactcaa ggcaagggcg aattcacgat ggagttcctc
      241 aaatacgcgc ccatcccaaa agggttgcaa gaagagctgg tggttgacta tcaaaaaaag
      301 aagaacagtt gatttgctga tttgcttttc agaataaggc acggggcaga catttctgcc
      361 tgccccggac attgaaaaca gacgataagc cggattctgt acactcatgc gcgtggcgcg
      421 cggtgccgca ttcattcatc tgggacgatt gttgccaacc gcctcgtgca gtctacccac
      481 cccgcaaaaa ccgccatgat tggccataaa gccaaggctg gtcttgtgac cgggacagtc
      541 acgattctca aaagaaaatc tgcgaggcct atttgacctt gcaacccata gagtttaccc
      601 ttttcactac aaacacccac aaagcgatac gctttgcggg ctgtacttgg tttctgtggc
      661 actgttcctc ggcccttcaa gcaggaccgg acgggcgtta cccattatgg ttccctgtgt
      721 tgtccggact ttcctcccca atgccgtgag gcatcaaggc gaatacgtgt ctgttcttgc
      781 caccatgact ataggctaaa tcactctcca tatgcaagac gcctctctct ttccgttacc
      841 cactcctacg gtgtatcccc caggatacgt aacggcctct tttttgggcc ctgtgcgagt
      901 ggaatcccgt atattcacac agcacaaatg gtgtgcccaa aatcaaggag ttcatatgaa
      961 aaaacacaac gcaacgatcc aaacattagt cttggcattg tgcctgggac tgctattaaa
     1021 ctgcgggcaa agccctctcg acctggatga tacactggac ccaaacggaa ccgatgacaa
     1081 tgagctcgcc ggaaacacat ctgtcagcac agcctgtgct ggtctggatt tggtcgacgt
     1141 taccgaagac atcaatgaca acaccacgtg gaagggtgat acggtgtacg tgattcgtgc
     1201 ctggaatttt tacgtgaacg ccaccttggt catcgagccc ggtgcgatca tcaagtttca
     1261 tgcgacagat ggccccgaaa tggttttggg tggggccggg accattctgg cccaaggaac
     1321 agaggccagc cccatcatct tcacctccta taaagatgac acgcatggtt gcgacaataa
     1381 tggcgacggc acagactcac tccccgcggc caaggactgg ggcagtgtcg acaccaatgg
     1441 cctcaacggc tcaatctttg accactgtga attctattat ggcggtgaca cagcctacag
     1501 tgccacactg gccctttcag cgggttctat tgccaccgtg atcaatagca cttttgcgca
     1561 caacgatggc ggtgatgaat caggctggta cggtgcattt gacgcctcgc acgccgaggc
     1621 gggaacagtg attcaaaaca acgtttttta tgataacgtg agacccctct caatcagtgt
     1681 ggc
//
LOCUS       DATDUN010000564         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_194790, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000564 DATDUN010000000
VERSION     DATDUN010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194790"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1204
                     /gene="prfA"
                     /locus_tag="VJC18_07910"
     CDS             143..1204
                     /gene="prfA"
                     /locus_tag="VJC18_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HLD45344.1"
                     /translation="MFTKLEQVEQKFEDLTQRMASGTLAPRDLTAMAKEQARLRETVT
                     TYREYKKNTQELAESKSLLEKEKDDDMRVMAREEVTELEKRQSEFEKKLKLLLIPTDP
                     NDEKNIILEIRAGTGGEEAGLFVADLFRMYTRFADSQGWKIDIVSSNETDKGGFKEII
                     AMVTGDRVYSKLKYESGIHRVQRVPDTEASGRIHTSAVSVAILPEAEDIELDIPPTDL
                     RIDVMRAGGAGGQHVNKTESAVRITHLPTNLVVVCMDDRSQHKNKAQAMKILRSRLLD
                     KMQQEQHAEEAQKRKTMVGSGDRSEKIRTYNFPQSRVTDHRIGLTVHALDAVLNGDLN
                     RLCDPLQAYYQAEAMKGGV"
     gene            1341..2327
                     /locus_tag="VJC18_07915"
     CDS             1341..2327
                     /locus_tag="VJC18_07915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HLD45345.1"
                     /translation="MSDPWTILRIIQWTIDYFKQKGFDTPRLDAEVIIAHALGLKKID
                     LYLQFERLLQEDELAHVKTLIKRRINHEPVAYLTGKKEFWSREFVIDENVFIPRPDTE
                     IVVEQVLEQVKKRGWQDNKLLGFEACLGSGCLAITLCCELPQLTMQAIEISPKAIEIA
                     KKNAEKFGVVERLNIVKGDVFRLKTKDQRPTSGSQGHGVDGSQDQCQETSASAALRSF
                     CPPFDFLIANPPYIALSEKDQLPQSVRGHEPHLALFADNEGLAFYPKIAEFAKNYLRD
                     DGFVAVEIGEQQGRAVKDIFESADLHHVMVKKDYAGKERVVIGNRESLIVNR"
BASE COUNT          665 a          577 c          670 g          569 t
ORIGIN      
        1 tgggtcgtga atcgtggatc gaaaagagtg tcattccagc tttcgctgga atccagaatt
       61 gaaaacatat ggatccctgc cttcgcaggg atgacaaaat aaaccgcgat ccacgattca
      121 cgattcacga ttcacgtccc ccatgtttac aaaactcgaa caagttgagc agaaattcga
      181 agacctcacg caacgcatgg cgtcaggcac gcttgcgccc agggatctga cggcaatggc
      241 caaggaacaa gcccgtcttc gtgaaacggt aacaacgtac cgcgagtaca aaaaaaacac
      301 acaggaactg gccgagagca aatctctttt ggaaaaagaa aaagacgatg acatgcgcgt
      361 gatggcgcgt gaggaagtca cagagctcga gaaaagacaa agtgagtttg aaaaaaaact
      421 caaactcctg ctgatcccca ctgaccccaa cgacgaaaaa aacatcattc tggaaatcag
      481 ggcagggacc ggaggtgaag aggcaggcct ctttgtggcc gacctgttcc gcatgtacac
      541 ccgttttgcc gactcgcagg gctggaagat cgatatcgtg tcatccaatg aaaccgacaa
      601 gggcgggttc aaggaaatca ttgcgatggt gaccggcgat cgtgtgtatt ccaaattgaa
      661 atacgagagt ggcattcatc gtgtgcaaag ggttcccgat accgaggcca gcgggcgtat
      721 tcacacctcg gctgtttctg tggccatttt gcctgaggcg gaagacattg aactcgacat
      781 cccccccacg gatctgcgca ttgatgtcat gcgcgcgggg ggggctggtg gccagcatgt
      841 gaacaaaaca gaatctgccg tgcgcatcac gcatttgccg accaatctgg ttgtggtgtg
      901 catggatgac cggtcgcaac acaagaataa ggcgcaggcg atgaagattt tgcgatcccg
      961 tcttttggat aagatgcaac aggaacagca cgccgaagaa gcccaaaaac gcaaaaccat
     1021 ggtggggtct ggtgaccgca gtgaaaaaat ccgcacctac aattttcccc agtcccgcgt
     1081 gacggatcac cgtattggtt tgacggtgca tgcccttgac gccgttctga acggcgatct
     1141 caaccggctc tgtgacccgc tccaagccta ttatcaggcg gaggcgatga aggggggggt
     1201 gtaaatcgtg gatcgtggat cgtggatcgt gaatcgcgga tccgtagctt tcgtcattgc
     1261 gagattcaca acgtgattcg aagcaatcca tttgaacatt tccattgcca ctttgttagc
     1321 cttatgattg tttcacgacg atgtcagatc cttggacaat attgcgcatc attcagtgga
     1381 cgattgatta tttcaaacaa aaaggtttcg acaccccgcg tctggatgct gaggtgatca
     1441 tcgcgcatgc cctgggcttg aaaaagattg atctctacct gcagtttgag cgcctgctcc
     1501 aagaggatga gcttgcccat gtcaagaccc tgatcaagcg gcgcatcaac cacgaaccgg
     1561 tcgcctacct gaccggcaaa aaagaattct ggtcacgtga attcgtaatc gatgaaaatg
     1621 tctttatccc gcgtcccgat acagaaatag ttgtcgaaca ggttttggaa caagtcaaga
     1681 aaaggggatg gcaggataat aaactcttgg gattcgaggc gtgtctggga tcaggttgtc
     1741 tggcgatcac cctttgttgt gaattaccac agttgaccat gcaggcgatc gaaatttccc
     1801 ccaaggcaat cgaaatcgcc aagaaaaatg cggaaaaatt tggggtggtg gaaaggctca
     1861 atattgtaaa aggggacgta tttagattaa agaccaaaga ccaaagacca acgtcagggt
     1921 cgcagggtca cggggtcgat gggtcgcagg atcaatgcca ggaaaccagt gcttctgcgg
     1981 ccctgagatc cttctgcccc ccatttgact ttcttatcgc caatcccccc tacattgccc
     2041 tgtccgaaaa ggaccagctt ccccagtcgg taagagggca tgaacctcac ttagccctgt
     2101 ttgctgataa tgagggactc gccttttatc caaaaattgc ggaatttgcg aaaaactatt
     2161 tacgagatga cggatttgtt gcagtagaaa taggcgaaca acagggaagg gctgtgaagg
     2221 acatttttga gagcgctgac cttcatcacg tgatggtcaa aaaagattat gcggggaagg
     2281 agagggtggt gatcgggaat cgtgaatcgt tgatcgtgaa tcgttgatcg tgaatcgttg
     2341 atcgtggatc gttgatcgtt gatcgttgat cgtgaatcgt gaatcgcttg tgcccttgag
     2401 ggtatgaatc gttgatcgtt gatcgtggat cgtggatcgt ggtttgtttt gtcatccctg
     2461 cggatgcagg gatccatgta t
//
LOCUS       DATDUN010000565         3382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_563503, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000565 DATDUN010000000
VERSION     DATDUN010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563503"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VJC18_07920"
     CDS             complement(<1..1588)
                     /locus_tag="VJC18_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45346.1"
                     /translation="MKNSKYIFYAVCFCLMLTSCDGVFTQYLFDAVNTDKAVDDVIVP
                     SEKSDEESGGLAPDLEETPTVGSSTTAGNPNLTTLIAYAPDSEDYIPVTGSVSEFASG
                     DMVAVSAVSGSVSMNECEERAYVRTSVRAYVEEMFANVNTHARQHVNTWFVPSAHASE
                     TACEDGETVKYCPIRDDGMFECFGKFADASVASLNVTVVDSECNNKSGTATVSILGGL
                     HYLGNTASDVTVADGAVYALADPDGDETTANSAIKLAEGADSRLATQGDHSSAVAYEE
                     RAANGSSFAFDGTDQNCFAVLNSTGGINTSSLADKSTCGTRVITGAKAYTKIKNTLQG
                     DVYFGIDVDDPRTEAVETTTEASIYYYHESDEKLDMEDRTYEDMFFIASGDRYMVEVA
                     PDHYVPTVPMRHKQTVDFDINSLGQALVLFIDEAGNYRLRGTTAGGTMRFGGEEASLG
                     NTTDHEFSDVNFIDDTTAMLLDTKNDELLFKHFDVAKHRIRGITSTVAEDLAGCVDKA
                     SAVLNEERSRLYLMCRSTNEI"
     gene            complement(1877..2056)
                     /locus_tag="VJC18_07925"
     CDS             complement(1877..2056)
                     /locus_tag="VJC18_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLD45347.1"
                     /translation="MKRKQLIRHLEKQGCVFLREGANHTVYFNPKTRMTSTVPRHNEI
                     NDFLAKKICKDLGLN"
     gene            complement(2058..2291)
                     /locus_tag="VJC18_07930"
     CDS             complement(2058..2291)
                     /locus_tag="VJC18_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLD45348.1"
                     /translation="MKEPKSNQFTAVYEKQGKYYIGYVEEIPGANAQGKTLSEVKKNL
                     KEAILLIIETNRIISLSGRQNSTSIREPITVRQ"
BASE COUNT          916 a          864 c          699 g          903 t
ORIGIN      
        1 aaatctcgtt ggtgctccgg cacatcagat acaggcgaga gcgttcttcg ttaagcaccg
       61 cgcttgcctt atcgacacaa ccggccagat cctcggccac agtggatgtg atgcctctga
      121 ttctatgttt ggcaacatca aagtgcttga acagaagttc atcgttcttt gtgtctagga
      181 gcatggcagt cgtgtcgtct ataaagttca cgtctgaaaa ttcatgatcg gtcgtgttgc
      241 caagagaggc ctcttcaccc ccaaaccgca tggtcccacc ggctgtggtg ccacggagtc
      301 tgtaattgcc agcttcatca ataaataaca ccaaggcctg ccctagtgaa ttgatgtcga
      361 aatccacggt ctgtttgtgt cgcatcggca cggtcggcac ataatgatcg ggcgcaacct
      421 caaccatgta ccgatcgcct gatgcaataa aaaacatgtc ttcatatgtt ctatcttcca
      481 tatcaagttt ttcatctgac tcatgataat aataaatact cgcctcagtc gttgtttcga
      541 cagcttctgt tcgtggatcg tccacatcta taccaaaata cacatcgccc tgcagtgtgt
      601 tttttatttt agtgtacgcc ttggcaccag tgattacacg cgttccgcag gtacttttat
      661 cggcaaggct actcgtgttg atcccgcccg tgctgttgag cacggcaaaa caattttgat
      721 cggtgccatc aaacgcaaag gaactcccgt tggccgcacg ctcctcgtac gccacagccg
      781 agctgtgatc gccctgcgtg gccaaacgac tgtctgcacc ctccgccaac ttgatggcag
      841 aatttgccgt ggtctcatca ccatcaggat cggccagagc atacaccgca ccatcggcca
      901 ccgtcacatc gctcgccgtg ttgcccaaat aatgcaggcc acccaatatg gacacagtgg
      961 ccgtgcccga tttgttattg cactcactat cgaccaccgt cacattgagg ctcgccacac
     1021 tcgcgtcggc gaacttgcca aaacactcaa acatcccgtc gtcgcgtatg ggacaatact
     1081 tcaccgtctc gccatcttcg caggcagtct cgctcgcgtg ggcggagggg acaaaccacg
     1141 tgttgacgtg ttgacgtgcg tgcgtgttta cgtttgcgaa catttcttca acgtacgcac
     1201 gtacggacgt acgcacgtac gcacgctcct cacactcatt catcgataca ctgccgctaa
     1261 ccgcactcac cgccaccata tcgccgctgg caaactcact cacagagcca gtcacaggaa
     1321 tgtaatcttc ggaatccggg gcgtaagcga tgagcgttgt caaattggga ttgcctgcgg
     1381 ttgtgctgga accaacggtt ggtgtttctt caagatcggg agcgagcccg ccactctcct
     1441 cgtcactttt ttcgctggga actattacat cgtccacagc tttgtctgtg ttcacagcat
     1501 caaacaaata ctgcgtgaac acaccatcgc acgaggtcag cattaaacaa aaacacaccg
     1561 cataaaaaat gtatttgctg tttttcatgg ccacctccta cacacagaca ccaacacttc
     1621 tcggctgtac tctacgaaag ttgtgcaaaa ttgtgaacca aaattggggt tttgtagcaa
     1681 aactgtattc cacagaacac accgtgccaa gtgttttgtc atcgcaaaac gcgatatttc
     1741 gggcatcacc gtgtcgattt caccggccaa gtttggtttc atcaagcaaa agcgcaaagt
     1801 taagacgatt ttttaacgat tgaccgcgtg tgtcagttga acatccagtt ttttgaaatt
     1861 gcggggaagt gatgtctcag ttgagcccga gatccttgca gatttttttt gccagaaaat
     1921 cattgatttc gttatgccgg ggcacagtgg aggtcatccg tgtcttgggg ttaaaataca
     1981 cagtatgatt cgcaccctcc cttaaaaaaa cacagccttg tttttccaag tgacgtataa
     2041 gctgttttct tttcatgtca ttgtctcact gtgattggtt cacgaataga ggtggaattt
     2101 tgcctaccac tgagagaaat aattctgttg gtttcaataa tgagaagaat ggcttctttc
     2161 aggttttttt tgacctcact tagggttttt ccttgggcat tggcgccggg aatttcttcg
     2221 acataaccaa tgtaatattt gccttgtttt tcatagacag cggtaaattg gtttgatttg
     2281 ggttctttca ttgtatttcc attataatcg gcttttgcaa aatactcaaa atttcttttt
     2341 cgtttctttc agcaccctca gccactctgt acggccgaat gaaaacattc aataagccag
     2401 ccccttccct ttgagctttg cctgccctac gccccctgcc ctgagccatt cgacaagctc
     2461 atggtaaact ttgtcgaagg gttacgcctt cttcgtgaac acttgaacac ctgaacacgc
     2521 ctgccccctg cgggggtgaa cacttgaaca cgaaatataa ctcatcgcgt catttattaa
     2581 ctctgtcctt gacacctaaa agcagattct ttggcaaaat caatcgattg ccatacacgt
     2641 ttgccatagg cctaacaaac cataataaca aagaaactca ccatctatcc acaagacaaa
     2701 aacagattat ttgtcaaatt tcaaaaaagc gtcacaatac acgcttcgtc aaaaaaccgt
     2761 caaagacctg ccgtaacctg ttgaaaagca ttgatattta gcaaaaaaaa cgctaactat
     2821 cgaccaaggt ttaataaata aattaataat ctttttcgca acaacaacag catttgtgac
     2881 gataacaatg tgtcaagcgt aggcaaaaaa gatttttatt attggaggct tgaataaaaa
     2941 aaatggtgat cttgaaagtt gcatggcaca acaatcaagt cgaatgaatc atctgtgttt
     3001 ctgagctagg tttgttcgca ataaaaaaat aacagtgccg cgtctcagca ccaacaacca
     3061 cgcaactgag ccgcccagtc aaccatcaag atcgcataga gcctatttac tgtacgacac
     3121 gtgcttgctc tgaagttcgt tcgtatttgt atgaaacagc tgatcaaaca acaacagggc
     3181 aagcgaagga gttagtaaat aatataagaa aacaaatgaa ttaaacacaa cctgctggag
     3241 aactttgtta agcaacgcca ctgcactaaa aagaggtgaa gtatgacgaa ctccattaac
     3301 aaagaccgtt tccccaacat cgatgcctct gttcatcaga acgcagatgt cgatttgtcg
     3361 aatcgtcacg gtgaatctgc cc
//
LOCUS       DATDUN010000566         1224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_485398, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000566 DATDUN010000000
VERSION     DATDUN010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485398"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VJC18_07935"
     CDS             complement(<1..696)
                     /locus_tag="VJC18_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45349.1"
                     /translation="MSHAEQREIIRVKKNPPLPVVAWEKFAFFLIVLAVLCAALVGTY
                     IYNATRSPAYEVAVEMGVNPQRTNDQGAVAPFPYYQNKALLQDHREGLLSSEVLARVV
                     SRPELLSIVEKEQLTPDLWKPTLSFGGTVAYLLGAEAPVFDAHAVPQTALTERILRGS
                     AVSFDPRSQLITLRVTDYNPRFAWFVATNVTEVYREFLKNKMDTTSKPPAVASKTASI
                     LSDPTGEASVPELG"
     gene            complement(748..>1224)
                     /locus_tag="VJC18_07940"
     CDS             complement(748..>1224)
                     /locus_tag="VJC18_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HLD45350.1"
                     /translation="GGQNAALTEWQNKYEEVVRANQMQKEMYLAKAAEFENFKKRLQK
                     EHDEFAKYANEKLILELLPVIDSLEMTLSHVRGNDNDPIAEGVGMIHKNILQTLSKFG
                     VREVRGEGQAFDPHVQEAIGTEENDALGPDKVVTVHRSGFVLNGKVVRAALVTVSC"
BASE COUNT          294 a          342 c          273 g          315 t
ORIGIN      
        1 accaagctca ggaacgctcg cctctccggt cgggtcagaa agaatacttg ctgtctttga
       61 cgcaacagca ggaggttttg atgtcgtgtc cattttattt ttgagaaatt cccgatacac
      121 ctctgtcaca ttggtcgcca caaaccaggc aaatcgcggg ttatagtcgg tcacacgcaa
      181 cgtgatgagc tgactgcgag gatcgaatga tacagcggat cccctcaaga tcctttctgt
      241 cagggctgtt tgcggaacgg cgtgtgcatc aaagacaggt gcctccgctc ccaggaggta
      301 cgctacagtt cctccaaacg aaagcgttgg cttccaaaga tccggagtca attgttcttt
      361 ctcgacgatt gagagcaatt caggtctgga aacaactctc gccaacactt cggaggacaa
      421 caacccttcc ctgtggtcct gcaacagagc cttgttctga taatagggga aaggcgcaac
      481 cgcaccttga tcatttgttc tttgaggatt gacgcccatc tccacggcga cttcgtaggc
      541 cggcgaacga gttgcattat aaatgtacgt gcccaccagc gccgcacaca ggacagccag
      601 cacgatcagg aagaaggcaa atttctccca cgccacaaca ggcagtggtg gatttttttt
      661 gacacgaatg atttctcttt gttctgcgtg actcattttc tccggcaccc tgaaaaaatc
      721 atcccaaaaa acaaatcgac aaaatcttca gcacgacacc gtcaccaggg cggcccgtac
      781 aaccttgcca tttaacacaa aaccggaccg atggaccgta acgactttgt cgggtcccaa
      841 cgcgtcattt tcctccgtcc caatcgcctc ttgaacatgc gggtcgaaag cctgcccctc
      901 ccccctcacc tccctcacac caaacttgga caacgtttgc agaatgtttt tgtgaatcat
      961 gcctacgccc tcggcaatgg gatcgttatc gtttcctctc acgtgggaca gggtcatctc
     1021 caggctatca ataaccggca ggagttcaag gattaatttt tcgttggcat atttggcaaa
     1081 ctcatcgtgt tctttttgga gacgcttttt gaaattttcg aattctgcgg ccttggccag
     1141 gtacatttcc ttttgcattt gattggccct gaccacctct tcgtatttat tttgccattc
     1201 agtcaaagcg gcattttgac cacc
//
LOCUS       DATDUN010000567         1359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_30323, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000567 DATDUN010000000
VERSION     DATDUN010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30323"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..850
                     /locus_tag="VJC18_07945"
     CDS             65..850
                     /locus_tag="VJC18_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLD45351.1"
                     /translation="MEFKNILLNIENRIATITVNRPNVMNALNPEVLADLMQGLTEVT
                     RHRKEVGSVIITGAGEKAFVAGADIAAMLKINALEAEAFVRLGQRCMRLIENFEMPVV
                     AAVNGFALGGGMELALSCDFIYASKNARMGLPEVNLGLFPGFGGTQRLARLIGKAKAK
                     ELIYTAAVMTADQAYQIGVVNRVCEPEQLLAEVTKTLNTIMSKGPIAVKLAKRVINEG
                     TDLPINSGLLLEETQFPIIFATEDRIEGVTAFLEKRPAQFKGK"
     gene            complement(872..1282)
                     /locus_tag="VJC18_07950"
     CDS             complement(872..1282)
                     /locus_tag="VJC18_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45352.1"
                     /translation="MNSIRYACTGNLAKGGWCVWMINNPERVMTDVPRKLSGEQLRDM
                     LRKANLFEYGSALEKNLDRQDPAIMLQAAVFNCYGDILNKTSGTNPNGDFPPEKGEAI
                     FNLAMNAITRCLDDENFRKTQAQEFWTRVDSVLK"
BASE COUNT          324 a          366 c          337 g          332 t
ORIGIN      
        1 gatctatagg acttagaaga cctatgataa gtcctgtagg actcatacga cctgtaatat
       61 cattatggaa tttaaaaata tcttattgaa catcgaaaac cgtatcgcga ccatcaccgt
      121 caaccgtccc aatgtgatga acgcgctcaa ccctgaggtg ctggccgacc tcatgcaggg
      181 actcaccgaa gtcacacgcc atcgcaaaga ggttggctct gtcatcatca ccggtgcggg
      241 cgaaaaggcc tttgtggccg gagccgacat cgccgcgatg ctcaaaatca acgccctcga
      301 ggcagaggcc tttgtgaggc tcggccagcg ttgcatgcgg ctcatcgaga atttcgaaat
      361 gcccgttgtg gcggccgtta acggctttgc gttgggcggc ggtatggaac tggcgctttc
      421 gtgtgatttt atctacgcct caaagaacgc ccggatgggc ctgcccgagg tgaatcttgg
      481 tctgtttccc ggctttggcg gcacgcagag attggcacgc ctcattggca aagccaaggc
      541 caaggaactc atctacaccg cggcagtgat gactgccgat caggcgtatc agattggtgt
      601 ggtcaatcgt gtgtgtgagc cggagcaact ccttgccgaa gtcaccaaga ccctcaacac
      661 gatcatgtcc aagggcccca tcgccgtcaa actcgccaag cgcgtgatca atgaagggac
      721 tgacctcccc atcaacagcg gtcttctgct cgaagaaacg cagttcccga tcatctttgc
      781 caccgaagac cgcatcgagg gcgtcaccgc ctttttggaa aaacgcccgg cacagttcaa
      841 gggaaagtga ttttttagta ttactctctc gctatttcaa cacagagtcc actcttgtcc
      901 agaattcctg ggcttgagtt ttgcggaagt tttcatcatc aagacaacgg gtgattgcgt
      961 tcattgccaa attgaaaatt gcctcaccct tctcgggagg gaaatcaccg ttggggtttg
     1021 tgccggacgt cttgttgagg atgtccccat aacaattgaa cacggcggct tgaagcatga
     1081 tcgcgggatc ttgacgatca aggttttttt ctaacgcaga tccatactcg aacaaattcg
     1141 cttttcggag catgtccctc aattgctccc cgcttaactt gcgaggaacg tctgtcatga
     1201 ccctctctgg attgttaatc atccacacgc accagccacc ttttgccaaa ttaccggtac
     1261 aggcgtatct aattgagttc acgcctgtcc ggttaaataa atctataaat tgataaagtc
     1321 cgattctgtg ggcatttgcg ggttaccaca ctcgcgaac
//
LOCUS       DATDUN010000568         1273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_399051, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000568 DATDUN010000000
VERSION     DATDUN010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399051"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VJC18_07955"
     CDS             complement(<1..226)
                     /locus_tag="VJC18_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45353.1"
                     /translation="MSSVSSVSYNYDTAMETIRADVEAYCETHGIDPDEFLTNLDGNV
                     TSSIFDNPTLEAYFQLAYMQLVELLYPEVIN"
     gene            complement(223..882)
                     /locus_tag="VJC18_07960"
     CDS             complement(223..882)
                     /locus_tag="VJC18_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45354.1"
                     /translation="MAHKKEDMKPKTIGPKPLQKTVAAPTAQGATPAIDQEVKLPSFF
                     DKHPDVKARMVAYLKEIPREQKQKSLQDFYKFINGDLTWGEIRKISKRMQKELARIAF
                     LNFKMQRFEKAETLFKGLAVIDHTNWYYRSALGAVYQKKKLFEKAIDEYSTALELRES
                     EVTSLVNRGECFLQLRDFDSALGDFNSVVHLNLPDNNPWHIRARVLRQRVLMAKNEDN
                     K"
     gene            complement(989..>1273)
                     /locus_tag="VJC18_07965"
     CDS             complement(989..>1273)
                     /locus_tag="VJC18_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45355.1"
                     /translation="IEEGQKVLLEKTEENCKTLADLVDGIEDYNMEDFEDQAALKADE
                     YTIQNIKESQTVLDETLKLLSEFVESALELASSGIQRTSSATRTIIQNMG"
BASE COUNT          273 a          316 c          300 g          384 t
ORIGIN      
        1 tgttgatcac ttcggggtag aggagctcca ccaactgcat gtaggccaac tgaaagtagg
       61 cctcgagcgt ggggttgtcg aaaatgctgg aggtgacgtt gccatcgaga ttggtcagaa
      121 actcatcggg atcgattccg tgggtttcgc aatacgcttc cacatcggcc cgaatggttt
      181 ccatggccgt gtcatagtta taactgactg atgaaacact actcatttat tatcctcatt
      241 ttttgccatc agcacccttt gtcttaaaac gcgcgcccga atgtgccagg gattgttgtc
      301 gggcagattc aggtgcacga cactgttaaa atcgccaagg gcagaatcaa aatccctgag
      361 ctgaagaaaa cattctccgc gattgaccag gctggtgacc tcgctctctc tcaactcaag
      421 ggccgtgctg tactcgtcga tggctttttc gaagagtttt ttcttctgat acacggctcc
      481 caaggcagag cggtaatacc aattggtgtg gtcgatgacg gccaaaccct tgaagagcgt
      541 ctcggccttt tcgaacctct gcattttgaa attcaaaaag gcgatgcggg cgagttcctt
      601 ttgcatgcgt tttgatattt tgcggatctc tccccacgtg aggtccccgt tgatgaactt
      661 gtaaaagtcc tgcagacttt tttgtttttg ttcccgaggg atctctttaa ggtaggcgac
      721 catgcgggcc tttacgtctg ggtgcttgtc aaagaacgat ggcagtttaa cctcttgatc
      781 gatcgctggt gttgccccct gcgcagtggg cgcggccaca gttttttgta gtggcttcgg
      841 accgatcgtt ttcggtttca tgtcctcttt tttatgtgcc atagtagtgt tttgttgtag
      901 ttatttcgct ctcagcataa cagcaaacct ggtgaccagc aagcaaagtc tctctcacaa
      961 caaacaaaca ccacgcacct ggggccctct agcccatgtt ctgaataatt gttcttgttg
     1021 cactcgatgt cctctgaatg ccactgctgg ccagttcgag agcgctctct acaaattctg
     1081 ataacagctt cagggtttcg tccagaactg tttgtgattc cttgatgttc tgaattgtgt
     1141 actcgtctgc tttcaaagcg gcctggtcct caaaatcttc catgttataa tcttctatgc
     1201 catcaacgag gtccgcaagt gttttacaat tctcctctgt tttttccaaa aggacttttt
     1261 gcccctcttc gat
//
LOCUS       DATDUN010000569         1332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_278990, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000569 DATDUN010000000
VERSION     DATDUN010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278990"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>1332
                     /locus_tag="VJC18_07970"
     CDS             19..>1332
                     /locus_tag="VJC18_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLD45356.1"
                     /translation="MDETKRKEELIRAMQSLPPNTLLLISGNKSGTVYDRLSSLIKSM
                     GLEQRVFLLGKVPNADTLEKAYVQTRQQRVWPRTPNLMAVLTGLPFGENERQFRLVVG
                     ASASRMEGWGMALHEMMAGGYAIVSSKMVPAATTCNQQYPDAVTIVEEGSNLPSRFAD
                     AIKTLIDDPAKARFQSQDTKKFAQDYSWRKLTRRWLRDVDRVFGRQELKRLPIVPGRD
                     HTQITIAEKRHKPLVVVLCGGVGERFKAVTGGEPKQLACVIQPDRSMLQTTVDRLTGV
                     ASRDGRFPITPDRVLLVTGQDMVARVASQLPQIPRRNIIAEPMRRNTGPAAALGLHYA
                     QITDPNAPVVIVSADHLITPVDTFLDRIGVAIDVAEQHALMAVFGIDPGRFSAQEGTQ
                     YGWIKSGSPLDTAKGVSLVELFKEKPDNNTAQEYLSQGNFLVNSGM"
BASE COUNT          342 a          327 c          340 g          323 t
ORIGIN      
        1 attgttgaat gtagccgcat ggacgaaaca aaacgcaaag aagagctcat cagggcgatg
       61 cagtccttgc caccaaacac attgctgtta atttcgggca ataaaagtgg aaccgtatat
      121 gaccgtctga gttcattgat caaatccatg ggactcgaac agcgtgtgtt tttgttgggt
      181 aaagttccca atgccgatac gctcgaaaag gcctatgttc agacaaggca gcaacgtgtt
      241 tggccacgga caccaaacct catggctgtt ctcactggtt tgccttttgg cgaaaatgag
      301 cgacagtttc gtttggtggt tggtgccagt gcatcccgca tggaaggctg gggcatggcc
      361 ttgcacgaaa tgatggccgg tgggtatgcc attgtctcat caaaaatggt gcctgccgct
      421 acaacctgca atcaacaata tccagacgcc gtaacgattg ttgaagaagg tagcaatctg
      481 cccagtcgat ttgccgacgc aattaaaacc ctcattgatg accccgctaa ggctcgtttc
      541 cagtcccaag acacaaaaaa gtttgcacaa gattattcgt ggcgcaagct cacgcgcaga
      601 tggcttcgag atgtggaccg tgtttttggc cgacaagaac tcaaacgcct gccaattgta
      661 ccaggacgtg accacaccca aattaccatt gcagaaaaac gccacaaacc gctcgttgtt
      721 gttttgtgtg gtggggttgg tgagcgattc aaggcagtca caggtggcga acccaaacaa
      781 cttgcctgtg tgatccagcc agaccgttcc atgttgcaaa caactgtcga taggctgact
      841 ggtgtggcaa gcagagatgg cagatttccc attacacctg atcgtgtgtt gctggttact
      901 ggtcaagaca tggtggcacg cgttgccagc caactacccc agatccctcg acgtaacatc
      961 attgctgagc cgatgcgtcg caacactggt cctgccgccg ccttggggtt acattacgcg
     1021 cagatcactg accccaatgc ccctgttgtt attgtttctg ccgatcatct gatcactccc
     1081 gttgacacgt ttttggacag aattggtgtc gctatcgatg ttgcggaaca acatgcgctc
     1141 atggctgttt ttggtataga tcccggcaga ttttcagcac aagaaggcac acaatatggt
     1201 tggatcaaga gcggaagtcc cctcgacaca gcgaagggtg tgagtttggt tgagcttttt
     1261 aaagaaaaac cggacaacaa tacggcacag gaatacttga gccagggcaa ttttttagtc
     1321 aacagtggca tg
//
LOCUS       DATDUN010000570         1123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_485579, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000570 DATDUN010000000
VERSION     DATDUN010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485579"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..646)
                     /locus_tag="VJC18_07975"
     CDS             complement(8..646)
                     /locus_tag="VJC18_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLD45357.1"
                     /translation="MHKWPVRAGSIWNLHVLGVLGMLPQLSELHARRRRFGLTQSALA
                     LKTGVSQSMIAKIEAGSIVPSYENAKKLFDFFDSLSEQTDAKASDFMSKKVIGVGPDM
                     PLKDCVRMMKAGAVSQLPVLDDGRNVGTVSERVILERMNSAQDLNKVSVIPVRDVMMD
                     AMPIVKEETSFRAISALLEHNPGVLVAKKGKIVGIITKSDLLNAVIEGAAKN"
     gene            793..>1123
                     /locus_tag="VJC18_07980"
     CDS             793..>1123
                     /locus_tag="VJC18_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HLD45358.1"
                     /translation="MTDQIADAILDEMLKGDPNSRVAVEVATKTGLILVMGEVTTKTY
                     VDIQSIVRKTIREIGFANPNYGFDADACSVLISLSEQSSDIAQGVNECENHEQGAGDQ
                     GMMFGYAC"
BASE COUNT          291 a          302 c          259 g          271 t
ORIGIN      
        1 gagatgccta attttttgcc gccccctcaa tcaccgcatt caaaaggtct gattttgtta
       61 ttatcccgac gatcttcccc ttctttgcga caagcactcc gggattgtgc tccaagagag
      121 ccgagatcgc ccgaaaggac gtttcctctt tcactattgg catcgcgtcc atcatcacgt
      181 cccttacagg gatcacactc accttgttga ggtcctgggc gctgttcatc ctctcaagta
      241 tcacgcgctc tgaaaccgtg cccacattcc gcccatcatc gagcacgggc agctgcgaga
      301 ccgcgcctgc cttcatcatg cgcacgcaat ccttcagggg catgtctggc cccacgccga
      361 tcactttttt ggacatgaaa tccgatgcct ttgcatccgt ctgctcgctt agggaatcaa
      421 agaaatcgaa gagctttttc gcgttctcgt atgatggcac gatcgaacca gcctcgattt
      481 ttgcaatcat tgactggctc acgcctgtct ttaacgcaag tgcgctttgc gtgaggccaa
      541 agcgcctgcg gcgcgcgtgt aattcagaga gctgcggcaa catccccaaa acacccaaca
      601 catgcaaatt ccaaatactt ccggccctga caggccattt atgcaaaaac tccaactctt
      661 attccaatat gaataaaaca gatggaaatt attattaata tagcgccgcc ctagtgttgc
      721 agggagggat tattcttgga aagaaaatac tttttcacat cagagtccgt aacagaaggc
      781 catccggaca aaatgacaga ccagattgca gacgcaattc tggatgaaat gcttaaaggc
      841 gacccaaatt caagggtggc agtcgaagtt gccacaaaaa cagggctgat tttagtcatg
      901 ggtgaggtga caaccaagac ctatgttgac atacagtcca ttgtgagaaa aaccatcagg
      961 gaaatcggat ttgcaaaccc caactatggc tttgatgccg acgcatgctc cgtgctcatt
     1021 tctctttccg agcagagcag cgacattgcg cagggcgtaa atgagtgtga aaaccacgag
     1081 cagggggctg gagatcaggg catgatgttt ggctatgcct gca
//
LOCUS       DATDUN010000571         1378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_316162, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000571 DATDUN010000000
VERSION     DATDUN010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316162"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VJC18_07985"
     CDS             <1..433
                     /locus_tag="VJC18_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLD45359.1"
                     /translation="EQPISRLDHEGMQGMTKRVGRFHIKIAADESCRTVEDVERIKRN
                     QEAHVINIKFAKSGLVDSLRMIATAGDSLELMIGGMVETRLLMGFSAQVAAALGCFHF
                     VDLDSPHYLSSDPISGGARLSGATYSLSHLRAGHEAGLGVS"
     gene            complement(502..1050)
                     /locus_tag="VJC18_07990"
     CDS             complement(502..1050)
                     /locus_tag="VJC18_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HLD45360.1"
                     /translation="MKKIVLAITGASGALYAKQFIDFVRPLTEVQLDVVASANGMRIF
                     HDELSLDLGEYWPRVCANTNFDVPFVSGSACADVMVILPCSMGTLGRIAGGFSNDVIT
                     RTADVMLKERKKLILVPREMPFNQIHLKNMLTLDQAGAVILPAMPSFYSCPQTIEALS
                     QTVVTRVLDHAGIPNELMKRWG"
     gene            complement(1105..>1378)
                     /locus_tag="VJC18_07995"
     CDS             complement(1105..>1378)
                     /locus_tag="VJC18_07995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLD45361.1"
                     /translation="QGGQVIVLDLFKPETGVGRLFYNTYGRFVLPLVGRLISRHEGAY
                     DYLRNSVQGFVSVGEFEKLVMKIGFASVRSRHFLFRAASVVCVNKN"
BASE COUNT          364 a          380 c          328 g          306 t
ORIGIN      
        1 tgaacaaccc atcagtcgcc tcgaccatga aggcatgcag ggcatgacaa aacgtgtggg
       61 aagattccat attaaaatcg ccgctgatga atcctgccgg acagtggagg atgtggagag
      121 aatcaaaaga aatcaggaag ctcatgtgat caacatcaag tttgccaagt ctggtttggt
      181 tgactcactc aggatgatcg ctactgccgg cgattctctg gaactgatga tcggcggcat
      241 ggtcgaaaca cgactcctca tgggattttc ggctcaagtg gccgcggcac tgggttgctt
      301 tcattttgtt gatctcgatt caccacatta tctcagctcc gatcccatca gcggtggtgc
      361 cagactcagc ggcgccacgt acagcctgtc gcaccttaga gcgggccacg aagcagggct
      421 aggggtcagc taggggtcaa gtctctggtt gactagttag tgatccaaga accaacaact
      481 agtcaaccag agacttgacc cctagcccca cctcttcatc aattcattgg gaatgcctgc
      541 gtgatccagc acgcgggtga cgactgtttg cgacagggct tcaatcgttt ggggacagga
      601 ataaaaacta ggcatggccg gcagaatcac ggccccagcc tgatccaagg tcagcatgtt
      661 tttgagatga atctgattga aaggcatctc acgcggaacc aaaatgagtt tttttcgctc
      721 tttcaacatc acatcggcgg tccttgtgat cacatcgttc gaaaaacctc cggcaatccg
      781 tcccagcgtg cccatggaac agggcaaaat caccatgacg tccgcgcatg cggaaccgct
      841 cacaaagggc acatcaaaat ttgtatttgc gcatacgcgt ggccaatatt ccccaagatc
      901 gaggctcaat tcatcgtgaa aaatacgcat cccattggca gacgccacca catccagttg
      961 aacctcggtg agaggccgca caaaatcaat gaactgtttg gcgtagaggg cgccagacgc
     1021 gcctgtgatg gccagtacga tttttttcat atgtttttcc cgatcgtcat cgccatgacg
     1081 taggggccgg gctggaccgg ccctctaatt tttattaaca caaacaacac tcgccgctct
     1141 gaaaagaaaa tgccgtgaac ggacgctggc aaaaccaatc ttcatcacca atttttcaaa
     1201 ctcaccgacg ctcacaaaac cctgcacaga attgcgcaaa tagtcatagg ccccttcatg
     1261 cctcgaaatc aggcgtccca ccagtgggag cacaaaccga ccatacgtgt tataaaacaa
     1321 tctgcccacc ccagtttcgg gtttgaacag atccagcaca atcacctgcc ctccttgt
//
LOCUS       DATDUN010000572         5783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_232242, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000572 DATDUN010000000
VERSION     DATDUN010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232242"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VJC18_08000"
     CDS             <1..780
                     /locus_tag="VJC18_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HLD45362.1"
                     /translation="VIKIGKFGAYLELTKDGELIKASIPDSLPPAEVTDQTIEELILL
                     KQTGPAVLGHHPQTSQPIFVLDGRYGPYVQLGDASEEKTKIKRVSLPKNLKPDQVTLA
                     IAVGLLALPRTVGLHPDSGARIFANIGRFGPFICLDRGKDGKDYRSLKISEGDDPTTV
                     TLSRALELFAQEKKTRGRSKAEPLKTIGSHPDDNEPVNLYHGPYGHYVKHKKTNASVP
                     KDVDINNIALAQALEWLEGRVGTKTRKRLKAKPFDKLRARS"
     gene            970..1626
                     /locus_tag="VJC18_08005"
     CDS             970..1626
                     /locus_tag="VJC18_08005"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLD45363.1"
                     /translation="MDLKKILDPNTRYIFLTTNEINHAFPVVHVKDNLIFFSNEKEIT
                     EDYQSGHFLAQGDEGIVLFTNPVVIPLKDVTKEEYGAFLYVINFDNTDYSITNRRLSA
                     RYEFKEFLPVSFQVYGDMMTAQLINISAGGLRLRVNTCLHRNVFCHFKITLPIQGKNV
                     CFETDGIVVYSEPEKSHNNFLTGISFVAPDFANKSEHQRYVEANAAIKKFVTEKIKQP
                     "
     gene            complement(1686..2093)
                     /locus_tag="VJC18_08010"
     CDS             complement(1686..2093)
                     /locus_tag="VJC18_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45364.1"
                     /translation="MLTRLASIILFCVFFVVVPTGVAILYDYFHRYKKAKRKYQRQFS
                     HCIGRHFIVVDQDALRGSVLDNNLSLGDVYQERGVIEYGFAVGDEIKILPYPKFQMFS
                     SKKVFFERVTRREQPNYEFDPEGQKLSFGVGKE"
     gene            complement(2206..2937)
                     /locus_tag="VJC18_08015"
     CDS             complement(2206..2937)
                     /locus_tag="VJC18_08015"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLD45365.1"
                     /translation="MKIVFRPLDQIEKLSNKPSSKARGQFDQMLGKHLDSARHAQALP
                     QSNLPPDQQRGVGERDWDFSENIEQLKDVDSEYYQKGVKKKTLAESFSARNDSKLEER
                     IKKFQPIIEAKAKKYDLDPKLLAGLIRQESNFNPYAVSHCGAMGLGQLMPETARYLGV
                     TDPFNAAQNLDGAAKYLKEQLNTFGGSVDKALAAYNAGPGAVQKYNGIPPYAETQNYV
                     RSIRSHTKQIAMMDIFPSEEPKKKA"
     gene            3197..3658
                     /locus_tag="VJC18_08020"
     CDS             3197..3658
                     /locus_tag="VJC18_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HLD45366.1"
                     /translation="MFYSHKAYLNAPEGSAVLVLTHDVKRALSDSKASKGHVHITSTS
                     GTTGIKLIENDSHLQKGYLTRIEKTFLDQPDNIVTRRSGTGPDKYHHMAVSCGLTLTL
                     PFENGRLLSSPFHEVVALDFEPKAGRREFIITIAADDAPAGAGRGQPMPRM"
     gene            3667..4659
                     /locus_tag="VJC18_08025"
     CDS             3667..4659
                     /locus_tag="VJC18_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HLD45367.1"
                     /translation="MSQHKTHTIPRSKPFCELGFENDVALQIFGPSEKHLKFIQKCVP
                     VTMKALGNRVCIEGDEKNIAVVEKSLLFLKDKVALGDPITRAHVIDALRCCQHDHTPE
                     LRIDMQKHVIIFPKKKIFPRTPGQLDYVRTIMSNDITLGIGPAGTGKTYLAMAVGMSK
                     LLRGEVERIILTRPAVEAGERLGFLPGDLAEKVNPYLRPLYDALHDMIDHERAARMMA
                     SGQIEIAPIAFMRGRTLNDSYVILDEAQNCTPEQMKMFLTRLGENSQAVVTGDITQID
                     LPCHTRSGLVQALSILKNINGIVIHEFDENDVVRHPLVVAIIKAYERAKKGKHV"
     gene            4652..>5783
                     /locus_tag="VJC18_08030"
     CDS             4652..>5783
                     /locus_tag="VJC18_08030"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45368.1"
                     /translation="MFDQNKNSHSHHLKGKKPGVVTNLIFLTTISLSVTALMVLYIDR
                     VPSNYHEGGIATQNIRADQNYEIIDHEATDKLKQQIAASSPPVYDFDVNLADARAEKI
                     QLGFQGARDVLEPLLKQRTSARDKLEDNIPLTTDEETQLRQGFETTLNTTLDEEIYTL
                     LRKNIFSPELQNTLITLSNHLQKRLIIHDTAELPQIPETGIVLRVLGTEDAVKEELVT
                     NFSHIMSLETAKKFFTPDDASLWLKELNLDFTDRDVMLTAIRIIPSLIKVDLTIDKSE
                     TEKRKERAIENVENIVHKLLKGQNIILRGQQYTHRHITILNGINETRLQTNILLKFLG
                     VFCLVFILLFTVYYYAHKHIVHFHLTRKDLNFIGLTLILFLAI"
BASE COUNT         1606 a         1575 c         1256 g         1346 t
ORIGIN      
        1 gtgatcaaaa ttggcaaatt tggcgcgtat ctcgagctca ccaaggatgg tgagctcatc
       61 aaggcctcga ttcccgacag cctcccgccc gcagaagtga ccgatcagac cattgaagaa
      121 ctgattttgc tcaaacagac ggggccggcc gttttgggtc accacccaca aaccagtcag
      181 cccatttttg ttttggatgg ccggtacggt ccgtatgtgc aactgggcga tgccagtgag
      241 gaaaaaacaa aaatcaaacg ggtgtcattg cccaaaaacc tcaaacccga ccaggtcacg
      301 ctcgccatcg ctgttggcct tttggctctt ccccgcacag tgggcctaca ccccgatagt
      361 ggggcccgta tttttgccaa cattggtcgt ttcggcccgt ttatttgtct cgaccgaggc
      421 aaggatggca aagactatag gtctctcaaa atttccgagg gtgatgaccc caccaccgtc
      481 accctgtccc gcgcgctgga actcttcgct caggaaaaaa agacgcgggg ccgctccaaa
      541 gcagaacctc ttaaaaccat tggctcacac ccggacgaca acgaacccgt caatctctat
      601 cacggtcctt atggacatta cgtgaagcac aaaaagacca atgcctctgt ccccaaagac
      661 gtcgacatca acaacatcgc gctcgcgcag gcgttggaat ggctggaggg gagggtgggc
      721 acgaagacac gaaaacggct caaagctaaa cccttcgaca agctcagggc aagaagctaa
      781 gagttcaaag ggagaaaact atcaatggct ccaattatca actacttcaa ttggcaattg
      841 ggtattgata attgatcgtt gaagcagttg tttttgcatt atgccttaca ccccttcgcg
      901 aacacgtgaa cacatgaaca cgtaaacacg tattgatatt ttccccatct tcgtgcatag
      961 aagccattca tggatctcaa aaaaatcctc gaccccaaca cccggtacat ttttctcacc
     1021 acgaacgaaa taaaccatgc cttccccgtc gtgcacgtga aggacaatct catttttttt
     1081 tccaacgaaa aagaaatcac agaagactat cagagcggcc attttctggc tcaaggggat
     1141 gagggcatcg ttttgtttac aaaccccgtg gtcatccctc tcaaagacgt gaccaaagag
     1201 gaatacggtg ctttcctcta tgtcatcaat tttgacaaca ctgactattc catcacgaac
     1261 cggcgtctca gcgcccgtta cgaattcaag gaatttttgc cagtgagttt tcaggtctac
     1321 ggcgacatga tgacagcaca gctcatcaac ataagtgccg gcgggctgcg tttaagggtc
     1381 aacacctgcc tccacaggaa cgtgttctgc cactttaaaa tcaccctccc catccaaggc
     1441 aagaacgttt gcttcgaaac ggatggcatt gtggtgtatt ccgaaccaga aaaatctcac
     1501 aacaattttc tcaccggcat cagttttgtg gcgcctgatt tcgcgaacaa atccgagcac
     1561 cagcgctatg tcgaggccaa tgccgccatc aaaaaatttg tgaccgaaaa aataaaacag
     1621 ccctagccat caaacaacca aattattttc gttcgaaacg aatcaggatc agaaccggaa
     1681 acccatcact ccttacccac accaaaactc agtttctgcc cttcggggtc aaattcgtag
     1741 ttgggctgtt ctctccttgt taccctctca aaaaaaacct ttttggaaga aaacatttga
     1801 aacttggggt agggtaaaat tttaatttca tcgcccactg caaagccgta ttcaatcacg
     1861 cctctttcct ggtacacatc acccagagaa agattgttgt ctaaaacact accccgtaga
     1921 gcatcctgat ctactacaat aaaatgtctg ccaatgcagt gactgaattg acgttgatat
     1981 tttcttttgg cctttttgta gcggtgaaaa taatcataca gaatagccac gcctgtggga
     2041 acaacaacaa aaaacacaca aaagagaatg atagaggcaa gtcgtgtcag catatgaggc
     2101 tctttacaat caatacccgc aaggcattac agtagcctgc aggccaagtc aaaataatcc
     2161 aatcactgcg tgtcctccgt cagagaatag ttctttcaag aatgatcagg ctttcttttt
     2221 aggttcttca gaggggaaaa tgtccatcat ggcaatctgt ttggtgtgag agcgaatgga
     2281 acggacataa ttttgagttt cggcgtaggg agggatgccg ttgtatttct gcactgcccc
     2341 gggtcccgcg ttataagccg ccaaggcctt gtcgacacta ccaccaaatg tgttgagttg
     2401 ttctttgagg tactttgccg caccatccag attctgtgcg gcattgaaag gatcggtaac
     2461 acccaaatac ctcgccgtct ccggcatgag ttggcccaaa cccatggccc cacaatgact
     2521 gaccgcatag gggttaaagt tggattcttg cctgatcagt ccggccaaca gcttgggatc
     2581 caaatcgtac ttctttgcct tggcttcgat gatgggttga aattttttaa tgcgctcttc
     2641 gagcttggaa tcgttacggg ccgaaaagga ttcggccagc gttttctttt taacaccttt
     2701 ttgataatat tcggaatcaa cgtcttttaa ttgttcgatg ttttccgaaa aatcccaatc
     2761 tctctcgcca acccccctct gctgatccgg tgggaggttg ctttggggta atgcctgggc
     2821 atgacgcgca ctatcgaggt gtttgcccag catttgatcg aactgccccc ttgcctttga
     2881 ggagggcttg ttcgataatt tttcgatctg atccagtggt ctgaaaacaa tcttcatttt
     2941 ttaatcccag aacgcaatag gggcagtccc ttcataacta ttatcgtccc ttgtcaccct
     3001 gagcgttgcg aaaaaagcca aatattttca aaattcccgc ctttttcatc cactcatcaa
     3061 tgatatcatg atgttagatt ttttcaaagc ctaatcgtga acacatgaac acgcctgccc
     3121 cctgcggggg tgaacacgtc tccctatgac ccagatttca tttgcacgtt gacgacaata
     3181 ttaatagaag agcatagtgt tttattcaca caaagcttac ctgaatgcgc cggaggggtc
     3241 tgccgttctg gtcttgacgc atgatgtcaa gagggccctg tctgactcga aggccagcaa
     3301 ggggcatgtc cacatcactt caacctccgg aacaacaggg atcaaactga tcgaaaatga
     3361 ttcacacctg caaaagggct atctgacccg tatcgaaaaa acatttttgg atcagcccga
     3421 caatatcgtc acacgaagat caggaacggg gccagacaaa taccatcaca tggcggtgtc
     3481 gtgtggactc accctcaccc tgcccttcga aaatgggaga ctgctttcaa gcccatttca
     3541 cgaagtggtg gccctggact ttgaacccaa ggctggccgc agggagttca tcatcaccat
     3601 tgccgccgat gatgccccgg caggcgctgg cagaggtcag cccatgccca gaatgtaaga
     3661 cgttcaatga gtcaacacaa aacccatacg atcccccgat caaaaccctt ttgtgaactt
     3721 ggtttcgaaa atgacgtcgc cttgcagatt tttggcccct cagaaaaaca cctcaagttc
     3781 attcaaaaat gtgttccggt caccatgaag gcgctaggca accgggtctg catagaaggg
     3841 gacgaaaaaa acattgctgt tgtcgaaaaa agtcttctgt ttctcaaaga caaggtcgct
     3901 ctgggtgacc ccatcacgag ggcacacgtg attgatgccc tgcgctgttg tcaacacgac
     3961 cacacccccg aactcagaat cgacatgcaa aaacatgtca tcatttttcc caagaaaaaa
     4021 atttttccca gaacacccgg acaattggat tacgtccgca cgatcatgtc aaacgacatc
     4081 accctcggca tcggtcctgc aggaacgggc aaaacctacc tggctatggc tgtgggcatg
     4141 tcaaaattgc tgcgcggaga agtcgaaaga atcattctca ccagacctgc cgttgaggct
     4201 ggtgaacggc tcggattttt acccggtgac ctggccgaga aggtgaaccc ctatttgcga
     4261 cccctgtacg acgccctgca cgacatgatt gatcacgaac gggcggcacg catgatggcc
     4321 agcggccaga tcgaaatcgc ccccatcgcc ttcatgcgtg gtcgcacact caacgacagc
     4381 tacgtcattc tggatgaggc tcagaattgc acgcctgagc aaatgaaaat gttcctcacg
     4441 cgtctgggtg aaaacagcca ggccgtggtc acgggcgata tcactcaaat cgatttgccc
     4501 tgtcacacac gatcgggact ggtacaagct ttgagcattt taaaaaatat caacggcatc
     4561 gtcattcatg agtttgacga aaacgatgtg gtcaggcacc ccctcgtggt cgccatcatc
     4621 aaggcctacg aacgtgccaa aaagggcaaa catgtttgat caaaacaaaa actcccactc
     4681 ccatcacctc aaaggaaaaa agccgggtgt ggtcacgaac ctgatcttcc tcaccaccat
     4741 ctccctgtct gtcacagcgc tcatggtgtt gtacatcgat cgcgtgccct caaattatca
     4801 cgagggcggg atcgccacgc aaaacatccg cgccgatcag aactacgaga tcatcgatca
     4861 cgaagccaca gacaaactca aacaacaaat cgccgccagc tcaccacctg tatacgattt
     4921 cgatgtgaac ctggccgacg cgcgcgccga aaaaatacaa ctgggttttc agggtgccag
     4981 agacgtactg gaaccactgc tcaaacagcg aacttccgcc cgcgacaaac ttgaagacaa
     5041 catcccactg acaaccgacg aagaaacgca actccgtcag ggattcgaaa caacactgaa
     5101 cacaacgctt gacgaggaaa tttacacatt actccgcaaa aacatttttt caccagaact
     5161 gcaaaacacg ctcatcaccc tgtccaatca cctgcaaaaa cgcctcatca tccacgacac
     5221 tgcggaactg cctcaaatac ccgaaaccgg catcgtcctg cgtgtgctcg gcacagagga
     5281 tgcggtcaaa gaagaattgg tcacaaattt ctctcacatc atgagtctgg agaccgcgaa
     5341 aaaatttttc acacccgatg atgccagcct gtggttgaaa gagttaaacc tcgattttac
     5401 cgacagggac gtcatgttga ccgccatcag gatcattccc agtctcatca aggttgatct
     5461 caccatcgat aaaagtgaga ccgaaaaacg aaaagaacgg gccatcgaaa atgtggaaaa
     5521 catcgtgcac aagctgttga aggggcaaaa catcattctg cgcggacaac aatacacgca
     5581 ccgacacatc acgatcctca acggcatcaa cgaaacacgc ctgcaaacca acatcctcct
     5641 gaaattcctc ggcgtcttct gcctggtttt catcctgctg ttcactgttt actattacgc
     5701 gcataaacac atcgtgcact ttcacctcac gcgtaaggac cttaatttca ttggcctcac
     5761 gctcattctg tttctggcca ttc
//
LOCUS       DATDUN010000573         1874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_524300, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000573 DATDUN010000000
VERSION     DATDUN010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524300"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VJC18_08035"
     CDS             <1..229
                     /locus_tag="VJC18_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HLD45369.1"
                     /translation="LYGSSAGITGTITAATIGTTVSGAAFKGPGTNYIAGETVRAAGD
                     VDADGYDDLIITATSTFTSTVQGKAYLVYGE"
     gene            complement(417..>1874)
                     /locus_tag="VJC18_08040"
     CDS             complement(417..>1874)
                     /locus_tag="VJC18_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLD45370.1"
                     /translation="PIMQVISAPEGVIVSQQAWSGDGLMMNSGDYTGETIEKGTQRIT
                     AWLEKNKLGKKQHQYKLRDWLFSRQRYWGEPFPILHDAEGNVHTLDPHDLPLTLPYLE
                     EFKPTADGQPPLAGATDWVHVTRDGNTFLRETNTMPQWAGSCWYYLRFMDPKNTSLPV
                     GKEAEKYWGPVDLYIGGVEHAVLHLLYARFWHKVLFDCGVVSTIEPFQKLFNQGMILA
                     YSYKDKNGKYHSPENVADQGKGQWVVKTSGEPVTSLVEKMSKSKLNVVNPNDVVAEFG
                     ADSLRLYEMFMGPLDREKPWSQQGLIGVFKFLSKAWRLIVDDQGKVLVDKSEPSEEVL
                     RLTHKTIKKVTSDIETLDFNTAISQLMIFVNELGRTNTRPLSVIKPFIQCLAPFAPHT
                     AEELWERIGGQGFVSIAAWPVFDEHLTKASKITIAVQVNGKTRETVDVDENTEQDEVL
                     ALAKETNHIKRQFELGQTIKKIIFVKNRILNLIMG"
BASE COUNT          400 a          484 c          501 g          489 t
ORIGIN      
        1 gttgtatggt tcaagcgcag gcatcactgg cacgatcaca gcggcgacaa tcggcacaac
       61 ggtgtcgggc gcggccttca aggggcctgg cacgaattac atcgctggtg aaactgtgcg
      121 ggcggcgggc gatgttgatg ccgatggtta tgatgatctc atcatcaccg cgaccagcac
      181 attcacctcc acagtgcaag gcaaggcata tctcgtttat ggtgagtgag tagttgtcta
      241 ttgacgcttg gtgtgcaatt tttactttgt aggggccggt cacgaccggc cccctacttc
      301 ccgtaaaaaa catgaacacg tgaacacatc ccgccaacgg gatcccgctc tgcggtgata
      361 aacacacgaa cacgcctgcc ccacaaagct cgaagagcgt agtggggtga gcacttctag
      421 cccataatca gattgagtat cctgtttttg acaaagatga tttttttgat cgtttgtccc
      481 agttcgaact ggcgtttgat gtgattggtt tctttggcga gcgccaacac ctcatcctgt
      541 tccgtgtttt catcgacatc caccgtctcg cgcgttttgc cattgacctg cacggcgatc
      601 gtgatcttgc tggccttggt cagatgctca tcaaacactg gccacgcggc aatcgatacg
      661 aatccctgtc cgccgatacg ctcccagagc tcctcggccg tgtgtggcgc aaatggcgcc
      721 agacactgga tgaagggctt gataacactg agcggacggg tattggttct gccaagctcg
      781 ttgacaaaga tcatgagttg gctgatggct gtgttgaaat ccagcgtctc gatgtcggag
      841 gtgacttttt tgatggtctt gtgtgtgagg cgcaggacct cttcgctggg ttccgatttg
      901 tcgaccagaa cttttccctg atcatcaaca atcaagcgcc acgccttgga taaaaatttg
      961 aatacaccga tgagtccttg ctgtgaccaa ggtttttcgc ggtccagcgg ccccatgaac
     1021 atttcgtaga ggcgcaaact gtcagcacca aattcggcca ccacatcgtt ggggttcacc
     1081 acgttgagtt tggacttgct cattttttcg accaggctgg tgacgggttc gccgcttgtt
     1141 ttgaccaccc attgtccctt gccctgatcg gccacattct caggtgaatg atatttgccg
     1201 tttttgtctt tgtacgaata ggccagaatc atcccctgat tgaacagttt ttgaaacggc
     1261 tcaatcgtgg agaccacacc gcaatcaaaa agaaccttgt gccaaaaacg cgcgtagagc
     1321 aaatgcaaaa cggcatgctc cacgccgcca atgtacagat ccacagggcc ccagtatttt
     1381 tcggcctctt tgccaacagg gagagatgtg ttttttggat ccataaaacg cagatagtac
     1441 cagcacgaac cggcccattg aggcatggtg ttggtttcgc gcagaaaggt attcccatcg
     1501 cgcgtgacat gtacccagtc tgtggcgccg gccagtggcg gttggccgtc tgctgtgggt
     1561 ttgaattctt cgagataggg cagggtgagt ggcagatcat ggggatcgag tgtgtgcacg
     1621 ttgccctcag catcgtgcag aattgggaaa ggctctcccc aatagcgttg gcgcgaaaat
     1681 agccagtcac gcaatttgta ttgatgttgt tttttgccca gtttgttttt ttcgagccag
     1741 gcggtgatgc gttgggttcc tttttctatg gtttcacctg tgtagtcccc tgagttcatc
     1801 atcagaccgt cgccgctcca ggcttgctga ctcacaataa cgccctctgg tgccgagatg
     1861 acctgcatga tggg
//
LOCUS       DATDUN010000574         3801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_360814, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000574 DATDUN010000000
VERSION     DATDUN010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360814"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VJC18_08045"
     CDS             complement(<1..772)
                     /locus_tag="VJC18_08045"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HLD45371.1"
                     /translation="MIGNLVCGHNKPMRKKKIIIGVFLMLALALVSQLGFAEGFKKLN
                     VSDKFGTDDTTPIAAPGTNEKAAPVTNPDNEPIYFNGVDVDIKDMINQISALTGKNFI
                     INDKVKGKITIISDKKMTRQEAYQAFLSALEINGFTTVMTPSGLINIVPQKDSLNKPL
                     EVFKEDSPNTDKFVTRIIQMKNISANEVSTLIKGMVSKSGNMFAYPTTNSIIITDTGS
                     NIQYLLSLIHELDQEGPQEAMEMIPIVNADAKDIADKIS"
     gene            complement(814..1752)
                     /gene="gspC"
                     /locus_tag="VJC18_08050"
     CDS             complement(814..1752)
                     /gene="gspC"
                     /locus_tag="VJC18_08050"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HLD45372.1"
                     /translation="MKKYIWVGGLVCVVIISYFLAKITTLIIEGYFPNVAITSSSEKS
                     SETTQLQQGTKVDVDLIISRNIFNAQETTTSSENATTETTGEDIQVSSDQAVETKLPI
                     KLIATVSVGDGKNSFSSCVIESNKAMDTYLVGDKKTFAPNTRIISILPKRVEFMNSDQ
                     LEYVKLEEFAKAVDLTKQPTKDPSNMITKVTQSEDDPGTGDDAIVRQGNTFQISRAEI
                     DGALTNVNKLFTDIRAVPFFEGGVAKGFKILSVKNGSLFEKLGIRRGDVLKSVNGRTL
                     DIQSGFETFGTLKNESEFELEINRRDEDVTYKYEVI"
     gene            complement(1924..2667)
                     /locus_tag="VJC18_08055"
     CDS             complement(1924..2667)
                     /locus_tag="VJC18_08055"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HLD45373.1"
                     /translation="MTKKILLTRSQDDNERMVAVLQTLGAEGISWPTFSYEATAIPET
                     DRNLLEHIDSYDWLIFTSAHAVHYFLKHFDALYVTRTQLQKIPVACVGDQTAAVANEN
                     GLAVSLVPPTFDVKHLANVPEFSGTHALRILIPQARDANHEALDLLALRHHIDSLVVY
                     EKQIVKHTTEEKDNLQKQMLDWIVFFSPSAIRFFMEDLTEECAKNILQRTPLAVIGPT
                     TATYLTQHGLSAQVVANRASAEALFRALL"
     gene            complement(2897..>3801)
                     /gene="dprA"
                     /locus_tag="VJC18_08060"
     CDS             complement(2897..>3801)
                     /gene="dprA"
                     /locus_tag="VJC18_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLD45374.1"
                     /translation="KLGIKIVNLHDDQYPYRLREIVNSPVQLFYRGSLEPLKASHVLA
                     IVGARRMTTYGKRVIEELIKPLVEKGIVIVSGMAFGVDVAGHRECLKQAGRTVAVQAQ
                     GVDKGYPRAHQGVFDQVIGSGGCVLSEFGLMNPDWNEKFLFPRRNRIISGLSDAVLIV
                     EAAEKSGALITARFASEQNRDVFAIPGSIHQPMSRGCLELIKQGAKPIITPEDIFEEF
                     ALRGAKTNKNQPADRTVIPKDLFESPLEENIFTVCRCDSKNLDDIIDAVSEPASFVTA
                     TVTKMLLLGRLREVEGKRFVSEEM"
BASE COUNT          926 a          975 c          899 g         1001 t
ORIGIN      
        1 gagagatctt gtccgcgatg tccttggcat cggcattcac gatggggatc atctccatgg
       61 cctcctgcgg accttcctga tccagttcat gaatgagcga gagcaggtat tggatgttgg
      121 acccggtatc ggtgatgatg atcgaatttg ttgtggggta cgcaaacatg ttcccactct
      181 tggacaccat ccctttgatc agagtcgaaa cctcattggc agagatgttt ttcatctgga
      241 tgatgcgcgt gacaaatttg tcggtgttgg gactgtcttc cttgaaaacc tcgagtggtt
      301 tgttcagcga atccttttgc ggcacaatgt tgatgaggcc cgaaggggtc atgacggtcg
      361 tgaagccgtt gatttccaac gccgacaaga aggcctgata agcctcctgc cgggtcattt
      421 ttttatcgga aatgatggtg attttgccct tcaccttgtc gttgatgata aaatttttgc
      481 cagtgagcgc cgagatctgg ttgatcatgt ccttgatatc gacatcgacc ccgttgaaat
      541 agatcggctc gttatcggga ttggtaacgg gcgcggcttt ttcgtttgtg cctggggccg
      601 caatgggagt cgtgtcatcg gtgccaaatt tgtcactcac attcagtttt ttgaatccct
      661 cagcgaagcc aagctgactc acaagcgcca gtgccagcat caaaaaaaca ccaatgatga
      721 ttttcttttt tctcatgggt ttgttgtgcc cacaaaccag attcccgatc atctattcgt
      781 gatcggcgtg ttggtttggt gactcacaaa taatcatatc acctcgtact tatacgtcac
      841 atcttcatca cgacggttga tttccagttc aaattccgac tcatttttga gtgtgccaaa
      901 cgtctcgaaa ccagactgaa tatccagcgt gcgcccattg acagatttga gcacatcacc
      961 gcgcctgatc cccaattttt caaacagaga cccgttcttc acgctcaata tcttaaatcc
     1021 cttggcaacc ccgccctcaa aaaaaggcac cgcgcggata tcggtaaaca gtttattcac
     1081 gttagtcaat gcgccatcaa tttccgcacg ggagatctga aatgtatttc cctgcctcac
     1141 aatggcatca tctcccgttc cgggatcgtc ttctgattgg gtgaccttgg tgatcatgtt
     1201 gctgggatct ttggttggct gtttggtcag atcaactgct ttggcaaatt cttcgagttt
     1261 gacatactcg agctgatcac tgttcatgaa ttccacacgt tttggcaata tgctgatgat
     1321 cctggtattg ggggcaaagg tttttttatc gccaaccaga tacgtatcca tcgctttgtt
     1381 cgattcaatc acacaagagc tgaaagaatt cttgccatcc cccacggaga ccgtggcaat
     1441 gagcttgatg ggcaacttgg tttcgacagc ctgatcggag gacacctgaa tgtcctcgcc
     1501 agtcgtttct gtggtcgcgt tttcggagga agttgtcgtc tcctgcgcgt tgaaaatatt
     1561 ccgactgatg atcagatcaa cgtcaacctt ggtaccctgt tgcaactggg tcgtctcact
     1621 tgatttttcg gaagacgatg tgattgccac gttgggaaag tacccctcaa tgatgagcgt
     1681 ggtgatcttg gccaaaaaat agctgatgat caccacacac acgagcccac caacccagat
     1741 gtattttttc aaaacagcac ccatgattca gacaactaat tgttgatcga tagtcagaca
     1801 aacactggtg tttgggaccg ctaccgacaa catccactcc ttggtgaatg attgccgatg
     1861 agggaacgaa tgggaaattc ttggtaacta tgccagattt ctaaacttta ggcaagcaga
     1921 acatcacaat aacgcccgaa acaaagcctc tgcgcttgcc ctgttggcaa cgacttgggc
     1981 agacaagccg tgttgcgtca aatacgttgc agtggtgggg ccgatcacgg ccaagggggt
     2041 acgctgtaaa atattcttgg cgcactcttc ggtgaggtct tccataaaaa aacgaatcgc
     2101 cgaggggctg aaaaaaacta tccaatccaa catttgtttt tgcaggttgt ctttttcctc
     2161 agtcgtgtgt ttgacgatct gtttttcgta caccaccaat gaatcaatgt gatgcctcag
     2221 agccaagaga tcgagggctt cgtggttggc gtctcgtgcc tggggaatca ggattcgcag
     2281 agcatgagtc ccggaaaatt caggaacatt cgccaaatgt ttaacgtcga atgtgggagg
     2341 aaccagtgaa actgctaatc cgttttcgtt tgccactgcg gctgtctgat cccccacaca
     2401 cgcaacaggg attttctgca actgggttcg tgtgacatac aaagcgtcaa agtgcttgag
     2461 aaaataatgc acggcatgcg cactggtaaa aatcaaccag tcatacgaat cgatgtgctc
     2521 caataagttt cgatctgttt cagggatggc cgtggcctcg taagaaaagg tgggccaact
     2581 gatgccctct gccccgagcg tttgcaaaac ggccaccatc ctttcattat catcttggga
     2641 gcgagtgagc agaatttttt ttgtcataaa taataatacg atatgggtct gtcattccga
     2701 cgaaggctag aggactgtca tccctgcgac ccttcgacag gcgctcaggg tggtgagcga
     2761 tttttcccga gtactgtcga gggaagtcga accaaggcag ggatccagat caaagcacac
     2821 atggatccca gcatgcgctg ggatgacaag aaacacccac ttcgacattc gatattcctt
     2881 gctcaatatt cgctattcac atctcttccg acacaaatct ctttccctca acttccctga
     2941 gcctgcccag gagcaacatt ttcgtcacgg tcgctgtgac gaaagaagcc ggttcggaca
     3001 cggcgtcgat gatgtcatcc agattttttg agtcacagcg acacactgta aaaatatttt
     3061 cttctagagg agattcgaaa agatccttgg gaatgaccgt tcgatccgcc ggctgatttt
     3121 tatttgtttt tgcccctctg agcgcgaact cttcgaaaat atcttccgga gtgatgatgg
     3181 gtttggcccc ctgcttgatc aactccagac aaccacgact catcggctgg tgaatcgagc
     3241 cgggaatcgc aaacacatca cgattttgtt cgctcgcaaa gcgcgctgtg atgagggcac
     3301 cggatttctc ggccgcctca acaatcaata cagcatcgct cagcccgctg atgatgcggt
     3361 tacgccgcgg aaacagaaat ttctcattcc agtccgggtt catcaacccg aattcggaca
     3421 acacacaccc cccagaccca atcacctgat caaacacgcc ttgatgcgcg cgggggtacc
     3481 ccttgtcgac accctgagcc tgcacagcta ccgtgcgccc agcctgcttc aaacactcac
     3541 gatgcccagc aacatccaca ccaaaggcca tcccgctcac aatcacaatg cctttttcta
     3601 caagtggctt gatgagttcc tctatcaccc gcttgccata agtcgtcatc cggcgggctc
     3661 ccacaatggc caagacatgc gacgccttga gaggctccag cgacccccga taaaaaagtt
     3721 gcacgggact attgactatt tcgcgcaggc ggtagggata ttgatcatcg tgcaaattga
     3781 caattttgat gcccagtttc g
//
LOCUS       DATDUN010000575         4613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_232314, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000575 DATDUN010000000
VERSION     DATDUN010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232314"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VJC18_08065"
     CDS             complement(<1..1576)
                     /locus_tag="VJC18_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HLD45375.1"
                     /translation="MKLEDIKPNTQITGIEAGQVVRVVSVEPAGDQAITVYYKTVDGR
                     LSERMLFRTDEARLSPAAAGRSWAFDASGEDFKLATEAYRINLAYLFDPMMAVHTSNV
                     VPLPHQITAVYESMLPKQPLRYVLADDPGAGKTIMAGLLIRELTIRADAKRVMIVAPG
                     SLVEQWQEELFEKFGLDFSPFTRGLEQQTLSSNPFEEKDFLIVRLDQLSRDEELQNKL
                     KNTYWDLIVVDEAHKMSANYYGNKLNTTKRFRLGMLLGSLTRHFLLMTATPHNGKNED
                     FQLFLSLLDSDRFYGKSRDGAHKVDVSDIMRRMVKEELLNFDGTKLFPERKAYAVNYK
                     LSDLEVALYHNVTEYVKEEMNKADNLDGQRKGTIGFALTALQRRLASSPEAIYQSLKR
                     RHKRLKNRVEEEKLRQRGQTLAETLFYDKVGKNVPEDLSEAADDLSAEEYENFEEEFV
                     DQATAAQTIQELEAEIEILKDLEEQAKQVVHSGQDRKWEELSKLLQDTPEMRDAEGRQ
                     RKLIIFTEHKDTLNYLA"
     gene            complement(1902..1974)
                     /locus_tag="VJC18_08070"
     tRNA            complement(1902..1974)
                     /locus_tag="VJC18_08070"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1939..1941),aa:Val,seq:cac)
     gene            complement(2025..3425)
                     /locus_tag="VJC18_08075"
     CDS             complement(2025..3425)
                     /locus_tag="VJC18_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD45376.1"
                     /translation="MTEANNTNVFKQTFKEVTEPFVGLVKASRALWAINLSYVVEGLC
                     YFGVLSLLAIYFNDFVGLNDIKAGQMVGMLTAGITLAMLFLGATVDWIGPRKSLLISL
                     AIMLVGRIILTSSTHVGAPGLWGTAHIVSILGMMGIVIGYGIYQPACYAAVKFFTTKE
                     TAAIGYAMLYALMNLGGFLPGLISPPLRKASGILGVFWVYVALTVVSILIIYFLLSRK
                     TEDKEMENFKKASETTKQSAEDNAIDQLKGKEKLKFYIKNFPLRDGRFMFFIFVLIPV
                     QTLFAHNWLTLPQYFSRAFTGVVQDNFEFFVNLNPILIFLLTPAIAVLTAKKDTYKMM
                     IIGTFVMSLPTFILTLGPSMYGVIAFIVLMTIGEAMWQPRFLQWIAEIAPKNMTGIYM
                     GIGQFPWFLTKVITSMYSGWFLMKYCPKDVPIDQLHTETMWLVYAFIAVSSPVGLIIA
                     RRWMGKDMERKLTQAA"
     gene            3610..4215
                     /locus_tag="VJC18_08080"
     CDS             3610..4215
                     /locus_tag="VJC18_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45377.1"
                     /translation="MIMASAPELFFTRFMDFYHPRTSPQATDPFVDLSETMGHRVHAQ
                     IHGATNDTLALAQSVPFREHRFVVAQVRADSVHSKPNTRRYDRRHNLFAGFQPLSNGQ
                     LRLFALERLFADHIPLHGRECLSIPYLGESHTLHLRLPNVGSFYLRMTKGALLTTVAE
                     LEGITPGTPLFGPFEVSSIFSAPALFEGARFFGPPELLPHP"
     gene            4292..>4613
                     /locus_tag="VJC18_08085"
     CDS             4292..>4613
                     /locus_tag="VJC18_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45378.1"
                     /translation="MNIQKPLSASLSALSLFCNPDLPDLLPPDVADHTARQASCQTAG
                     AQLAALRDGTLMTRQGLSGTRAHTFTSVVAPQVGDELIADAAELFAHLRDERTRVALL
                     KILAP"
BASE COUNT         1189 a         1210 c         1007 g         1207 t
ORIGIN      
        1 cggccaaata attaagcgtg tccttgtgtt ctgtaaaaat aatgagtttt ctttgcctgc
       61 cttcggcatc acgcatttcg ggagtgtctt ggaggagttt tgataattcc tcccatttac
      121 gatcttgacc agaatgcacg acctgttttg cctgctcttc taagtcttta agaatttcaa
      181 tttctgcttc cagttcctga atggtctgag cggcggttgc ctgatcaaca aattcttctt
      241 caaagttttc gtattcttct gcagaaaggt cgtcggccgc ttctgacaaa tcttcgggga
      301 catttttgcc cactttgtcg taaaaaagag tttcagccaa ggtctgacca cgctggcgca
      361 atttttcttc ttcgacacgg tttttaagtc ttttgtgtct tcgtttaaga gattgatata
      421 tggcctctgg acttgatgct agtctgcgct gcaacgctgt taaggcaaac ccaatagtcc
      481 ctttacgctg accatccagg ttatccgcct tgttcatttc ttctttaacg tattcagtca
      541 cgttgtgata cagggcaact tcaaggtcag acagtttgta atttacggca taggcctttc
      601 gctctggaaa caattttgtt ccatcaaagt tgagtaattc ctctttgacc atccggcgca
      661 tgatgtccga cacatcaact ttatgagcac catcacgcga ctttccataa aaacgatcag
      721 aatccaacaa tgacaagaag agctgaaaat cctcattctt gccgttgtgt ggtgttgctg
      781 tcatcagcaa aaaatgccgc gtaagcgacc ccaacaacat ccccagccta aaccgttttg
      841 tggtattgag tttgttgcca taataattgg cggacatctt atgggcttca tcaacaacta
      901 ttaaatccca atatgtattt ttgagtttat tttgtaattc ttcatctctc gacaactgat
      961 ccaaacgaac aataagaaaa tctttttctt caaaaggatt actgcttaaa gtttgctgtt
     1021 caagacctct ggtaaaggga gaaaaatcga gaccaaattt ttcaaacaat tcttcttgcc
     1081 actgttcaac caagcttccg ggagctacaa tcatgacgcg cttggcatcg gccctgatgg
     1141 ttagttctcg aataagcagg cctgccatga ttgttttacc cgccccagga tcatccgcca
     1201 aaacatagcg aagaggttgt tttggcaaca tggactcgta aacggctgtg atctggtggg
     1261 gcaatggcac aacattggat gtgtgcactg ccatcatggg atcaaataaa tatgccaaat
     1321 ttattcgata ggcttcggtt gccagcttaa aatcttctcc gcttgcatca aaggcccaag
     1381 accgacctgc cgctgccgga gagagtctag cttcatccgt tctaaacaac atgcgttccg
     1441 acaatcgacc atccactgtt ttgtaataaa cagtaatcgc ctgatcaccc gcaggttcta
     1501 cactaacaac acgcacgact tgtccagctt caatgccggt aatttgcgtg ttgggtttta
     1561 tgtcttcaag tttcatccgt gttttactcc aataatctgc accacagtta tgtttggcag
     1621 cagtgtcaag caactaacaa atactttctc aaaactcaaa ccgagacttc gttaccccac
     1681 aagcccctcc aattccttct tatatgtctt ctccaacaac atcacctgaa acacctccag
     1741 tctcacctga cctgttttaa tattggaaag gaaggattgc gatatgggaa agaaaggaaa
     1801 taggcagaaa actatcgtaa gggaaaacac tgcccgttcg actcccgctg gtcgctcagg
     1861 gcacaaattt cggccactgc gactgacgtg gtcgaaattt gtgggcatga caggatttga
     1921 acctgtgact cctaccgtgt gaaggtagta ctctagccac tgagttacat gccccgaccc
     1981 ctaaagtgat ctcacgatca cgcgggggtc cgctactaca aaatttatgc cgcctgagtc
     2041 aactttcgtt ccatgtcttt acccatccaa cgtctggcga tgatcaaacc cacagggctg
     2101 gatacggcaa tgaaagcata gacgagccac atcgtttctg tatgcaactg gtcgatgggc
     2161 acatctttgg gacagtactt catgaggaac cagcctgaat acatgctggt gatgaccttg
     2221 gtcaagaacc agggaaactg gccaatcccc atgtaaatgc ctgtcatgtt cttgggggca
     2281 atttcggcga tccactgcaa gaaacggggc tgccacatgg cctcaccgat cgtcatgagg
     2341 actatgaagg cgatcacgcc atacatgctc ggacccaacg tgaggataaa cgtgggcagg
     2401 gacatgacga aggtcccgat gatcatcatt ttatacgtat ccttcttggc cgtcagcaca
     2461 gcaatggcag gagtgagcag gaagataagg atgggattca ggtttacaaa aaattcaaaa
     2521 ttatcctgca ccacacctgt gaacgcccga ctgaaatact gtggcagggt cagccaattg
     2581 tgcgcaaaca acgtctgcac cggaatgagc acaaagataa aaaacatgaa ccggccatcc
     2641 ctgaggggaa aatttttgat gtagaacttg agtttctctt tacccttgag ttgatcaatc
     2701 gcgttgtcct cagcgctttg ttttgttgtc tcggaggcct ttttaaaatt ctccatttcc
     2761 ttgtcttcgg tttttcgcga cagtaaaaaa tagatgatca aaatgctcac caccgtcagg
     2821 gccacataga cccagaacac gcccaagatc ccagaggctt ttctgagggg gggggaaatg
     2881 agccccggca aaaaaccacc cagattcatg agcgcataaa gcatggcgta accaatggcc
     2941 gcggtttctt tggtggtaaa aaatttgacc gccgcgtaac aagctggctg gtagatccca
     3001 taaccgatca cgatccccat catccccagg atggatacga tgtgtgccgt cccccacaga
     3061 ccgggggccc ccacgtgagt cgatgatgtg aggatgatgc gcccaaccaa catgatggcc
     3121 agagaaatca gcagtgattt acgcgggcca atccaatcga ctgtggcacc caaaaaaagc
     3181 atggctaacg tgatgcccgc cgtgagcatg cccaccatct gtcctgcctt gatgtcgttg
     3241 agacctacaa aatcgttaaa ataaatggcc aaaagactca acacaccaaa gtagcacagg
     3301 ccttctacga cgtatgatag attgatagcc cacaatgcgc gtgaggcctt gaccaaaccc
     3361 acaaaaggct cggtcacctc cttgaaagtt tgtttaaaca cgttcgtatt atttgcttcc
     3421 gtcatatttt tctccagcaa aaggttactt gaaacacccc gtattgtgac tccagacgca
     3481 aaggggcctt ttccaacaac ctgcctcaag aatcaaggtg aatattttcc tctttttcta
     3541 ttgaattgcg atgggcaaaa agattagggg gcaatcagtg gtaactcccc cctcactgaa
     3601 aggacaacta tgatcatggc atccgctccc gaactcttct tcacacgatt catggacttt
     3661 tatcacccgc gcacttcacc gcaagccaca gacccgttcg tagacttatc ggagaccatg
     3721 ggtcaccgag tccatgcaca aatccacggt gcaactaacg acacgcttgc gttggctcaa
     3781 tctgtgccat ttcgtgaaca tcgttttgtc gtggctcaag tccgtgcgga ctctgtgcat
     3841 tcaaagccca atacccgcag atacgatagg cgtcacaatc tttttgcggg atttcaaccg
     3901 ctctcaaacg gtcagttgcg tctgtttgcc ttggagcgcc tgtttgcgga tcatattccc
     3961 ctccacggac gtgaatgctt aagtattccc tatctgggcg aaagccacac ccttcacttg
     4021 cgattgccaa atgtgggcag tttttatctg cgaatgacca aaggcgcgct cttgaccacg
     4081 gtggctgaac tcgaaggcat cacacccggc acccctctct ttgggccttt tgaggtgtct
     4141 tcaatcttta gtgcccctgc gttatttgaa ggggcccgct tttttggccc tcccgagtta
     4201 ttgccccatc cgtaacgctt agttggcaaa tttttcaagc aactcaacaa caaaacctgc
     4261 gataaggagt cctgttgggc aacgaccaaa tatgaatatc caaaaaccct tatcagcatc
     4321 cctctcagct ttgtcacttt tctgcaatcc tgatctgccc gatctgctcc ctcctgacgt
     4381 tgcagatcac actgcgcgac aagcttcctg ccaaaccgct ggcgcacaat tggcggccct
     4441 cagagacggc accctcatga cgcgtcaggg attatccgga acacgcgcac acacgtttac
     4501 cagcgttgtc gccccgcagg tgggtgatga gctgatcgcg gacgccgctg aattatttgc
     4561 gcacctgcga gatgagcgca cccgtgtggc cctgctaaaa attctggccc ccg
//
LOCUS       DATDUN010000576         2712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_232356, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000576 DATDUN010000000
VERSION     DATDUN010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232356"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..751
                     /locus_tag="VJC18_08090"
     CDS             344..751
                     /locus_tag="VJC18_08090"
                     /inference="COORDINATES: protein motif:HMM:NF002007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HLD45379.1"
                     /translation="MSQITWDSSYSVGIQKIDEQHKKLINLINHLDAAVGKGQGSAMQ
                     GDLIMELAEYLMDHFGMEEALMDKYGYPQYDAHLQIHHSFTRKVKEFRDDIAAGSLSV
                     SEEALVFLGDWLINHIQATDKLLGDYLREQGEV"
     gene            760..1164
                     /locus_tag="VJC18_08095"
     CDS             760..1164
                     /locus_tag="VJC18_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLD45380.1"
                     /translation="MTSKVLLHHLALKTHDCDKLKAFYCRLFKIKPTAERYDERGQLV
                     SVWLRFGATILMIERSTQKRVCPKQPRKFSDDPAGWHLTAFAIKPKDRSAWLAHLKQL
                     KIKIQKQTPYSLYFFDPDFNRLALSHYPKKAQ"
     gene            complement(1173..1451)
                     /locus_tag="VJC18_08100"
     CDS             complement(1173..1451)
                     /locus_tag="VJC18_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLD45381.1"
                     /translation="MSYRIFETRTFQKQLHQALKSGGYKKLDRKIREYVYPLLRAEPH
                     FGMQIKKLKDFDPETYRFRIGDFRLFYSIDEEDKVVVVTAIKLRGSAY"
     gene            complement(1484..1720)
                     /locus_tag="VJC18_08105"
     CDS             complement(1484..1720)
                     /locus_tag="VJC18_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HLD45382.1"
                     /translation="MQRTSITIKPTLLQTLNKLATDENRPLSNLIETILIRYLAEAFF
                     VDDFEMDEIRQDTALQKNIKSSLKEYKKKQGQFV"
     gene            1997..2500
                     /locus_tag="VJC18_08110"
     CDS             1997..2500
                     /locus_tag="VJC18_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45383.1"
                     /translation="MMTGFTSLPISTVSHELSLAVPGQWGRACSVALDGSNSYIPQGK
                     GVYPLLDSVYHRSHVLTGEGLMTWGQGEKPTLQRFQPLAVAQEQKVAFPRLLNLSLVD
                     QPPHVTPAFLVRSMESLQALPKVMRDFANRIETDPSFARIVTDQDLNLLRKVADTMER
                     VVFGVQA"
BASE COUNT          726 a          553 c          667 g          766 t
ORIGIN      
        1 cgggggccaa ggcaaaactg atgtatgaaa ccggcgatct ggatgtgggg gtgatatcat
       61 gcggacaggg cgtgggcttg gtgcacgata tcccgtccgt tgcggatttg tttgatcgca
      121 tcatgacaga gacggagcaa accattgaaa agtgtagtgc gatgcaactt taacaatgtc
      181 gcctaccatt ttggggtcct gctacggggc gctatttttt tgacttttca ctcgcaagct
      241 cgctccgtcc cgagtacggg actcagtcaa aaataatagc ttcccgtatc accccaaaat
      301 ggcggattat tcgaatgcta catattgatg aggaaaacac attatgtcac aaatcacatg
      361 ggacagttcg tacagtgtgg ggattcaaaa gatagatgag caacacaaaa agctcatcaa
      421 tctcatcaat catctcgatg cggctgtggg caagggtcag ggctctgcca tgcagggtga
      481 tttgatcatg gaactcgcgg agtatttgat ggatcatttt gggatggaag aggccctgat
      541 ggacaaatat ggctacccac agtacgatgc gcatctgcaa atccatcaca gttttacaag
      601 gaaggtcaaa gaattcaggg acgatattgc ggcggggagt ctttctgtga gtgaggaggc
      661 gctggttttt ttgggggatt ggttgatcaa tcacattcag gccaccgaca aactgttggg
      721 tgattatttg agagaacagg gggaggtgtg acttgcgcta tgacatcaaa agttctgttg
      781 catcatcttg ccctcaagac gcatgattgc gacaaattaa aggcgtttta ttgccgctta
      841 ttcaaaatca aacccacagc cgaacgctac gatgagcgtg gtcagctggt ctcggtctgg
      901 ttgcgttttg gtgccaccat tttgatgata gagcggtcca cacaaaaacg cgtttgccca
      961 aaacaacccc gtaaattttc tgatgaccct gcaggctggc atttgacagc ctttgccatc
     1021 aaacccaagg atcggtctgc ttggctggct catctgaagc agttgaaaat caaaattcaa
     1081 aaacaaaccc cgtattcgct gtattttttt gatccggatt ttaaccgcct ggccctgtcg
     1141 cattatccca agaaggccca atgaaaatag ttttaatacg ctgacccccg taatttaatg
     1201 gctgtgacca caactacttt atcttcttca tctatcgagt agaacagcct gaaatccccg
     1261 attctaaacc ggtaggtttc cggatcgaaa tccttcagct ttttgatctg cattccaaaa
     1321 tgtggttctg cgcgcaataa gggataaacg tattcacgga ttttgcgatc cagtttttta
     1381 tagccgccac ttttgagtgc ctgatgaagc tgtttttgaa atgttcgggt ttcgaatatt
     1441 cgatacgaca taaacctcgc cccttactgc ttctgatcgt cctttaaaca aattgcccct
     1501 gtttcttttt gtattccttg agagaggatt tgatattttt ttgcagagca gtgtcttgcc
     1561 ggatttcatc catctcaaaa tcatccacaa agaatgcctc ggccagataa cggatgagga
     1621 tggtttctat gaggttggag agcggcctat tttcatcggt agccagtttg ttcagggtct
     1681 gtaaaagagt gggcttgatg gtgatggatg tgcgttgcat gagttctcct gcaagtagat
     1741 gcattatgca tcagatagca tcattataca taattatgat gtatattgca acctttgtgt
     1801 tttggagggg gactcttgtc tgacgaaaca gttggttttg tcggtggttt tgttgtaaag
     1861 ccacacaaag cgcattattc atgagaatat ccggagatga tctcaagata tcaatgggtt
     1921 actgtgaata taaaattaat gagaattgtg gaaacttatg gggtgctggt gacgaagatc
     1981 gaccaaaggt gatggaatga tgacgggttt cacatcgcta cccattagca ccgtgtctca
     2041 cgagttgtca ctggctgtgc cgggacagtg ggggagggca tgctctgttg ccctcgatgg
     2101 ttcaaatagt tacatccctc aaggaaaggg tgtctatccg ttattagaca gtgtgtatca
     2161 tagaagccac gtcttgacgg gcgagggttt gatgacttgg ggacagggcg agaagccaac
     2221 attgcagaga tttcaaccgt tggctgtggc gcaagaacaa aaagtggcgt ttcctcgttt
     2281 gttgaatttg tctttagttg atcaaccacc tcatgtgacc ccggcgtttc tcgtcaggtc
     2341 aatggagtct ttgcaagcac tccccaaagt aatgagggac tttgcaaatc gtattgaaac
     2401 agacccctcg tttgcgcgca ttgttacaga tcaggatttg aatttactga gaaaagtggc
     2461 agacaccatg gaaagggttg ttttcggagt gcaagcgtaa atgccccaaa aatatttaac
     2521 aagcttgttg agtctgtgtt caaaacaata cagagtacag tgtttagcaa atataaaaca
     2581 tttataataa cagtaagata ggctttatca tgtgttcaaa tgaacactaa atcaatttta
     2641 atcagttgta cttgaacaca tgttggtggt atgatggggt catggcaaag cagaaaacca
     2701 ccatcaacat tt
//
LOCUS       DATDUN010000577         1349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_399233, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000577 DATDUN010000000
VERSION     DATDUN010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399233"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VJC18_08115"
     CDS             <1..876
                     /locus_tag="VJC18_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45384.1"
                     /translation="KPRVAGASLISHSLRPRDLHENQQVIYVRFPHLTQYPTDIIVAR
                     LKKLFSEVTVIDYSSWEAPVVSPLAGYLRHFDEQKNVEGLCELISRKAAQVPLIFLPP
                     VLGIKHHFVHHQLCQQKTGAKVVELLSTLPSSAGLRLTHLLDDRLNQLGIECVTGEAC
                     PFACTDDVSAGLPIRGGCQGGSDRPHLRANVYLLATGKYVGGGVKAQSFFSEPITRAP
                     LYLDGRAVSPSANMTQLVTPQADLSQDFMRLGVSLSQNSKNIKYCGHVLAGFDYTREG
                     CGFGVSMASALSCFS"
     gene            1067..>1349
                     /locus_tag="VJC18_08120"
     CDS             1067..>1349
                     /locus_tag="VJC18_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLD45385.1"
                     /translation="MGKPKTKNSQIEIQSIDELYGKVAQLEQLVETISRSKMMWESTF
                     DVISDPVLIINRNFEIKRANKALARACGVDIKEVIAKKCYKIFAGLEAPC"
BASE COUNT          329 a          299 c          354 g          367 t
ORIGIN      
        1 aaaccaaggg tggctggggc gtcattgata tcacactcct tgagaccgcg tgatctccac
       61 gaaaatcaac aggtcattta cgtgcgcttt ccccacctca cgcagtatcc cacggacatc
      121 attgtggccc ggcttaaaaa gttgttttcc gaagtgacgg tcattgatta ttcttcctgg
      181 gaggcccccg tcgtttcacc tctggctggt tacctgcgcc attttgacga acagaaaaac
      241 gtcgagggct tgtgtgagct gatttcgcga aaggccgctc aggtgccgtt gatcttcctg
      301 cctccggtgt taggaatcaa gcatcatttt gtgcatcatc agctttgtca gcaaaaaacg
      361 ggcgccaaag tcgtggagct tttatcgacc ctgccttcat ccgcaggtct gcggttgacg
      421 catcttctgg atgacagatt gaaccaactt gggatcgagt gtgtgacagg tgaggcctgt
      481 ccctttgcct gcacggacga tgtatcggcg ggtttgccaa tcaggggtgg ttgccaggga
      541 ggctccgaca ggccccattt gcgggccaat gtgtatctgt tggctacggg taaatatgtg
      601 ggtgggggag ttaaagccca gtcttttttt tcggaaccaa tcacaagggc ccctctgtat
      661 ctggatgggc gtgctgtttc tcccagcgcg aacatgacgc aacttgtgac tccccaggcc
      721 gatctttctc aggacttcat gaggttgggc gtgtcgcttt cgcaaaacag taaaaacatc
      781 aaatattgtg gtcatgtgtt ggcgggattc gattacacgc gggagggttg cgggtttggt
      841 gtcagcatgg cgtcagcctt atcatgtttt tcctgaaggg cttttcgtca aaatattatc
      901 aatcacaaaa catagtcaaa ataaacattg atcgttattt gccattatgc aagccttcaa
      961 aatggatcag gaaaaataat gtcaaaaaat tggtgatatg cttttgattt gatagtatat
     1021 gacagtaata tcatgacgta atgcgaaaaa cttaaaacga gtttttatgg gcaagccaaa
     1081 aacaaaaaat tctcaaattg agattcaatc gattgatgag ctgtatggca aggttgccca
     1141 gttggagcaa ttggttgaga ccatctcccg ttccaaaatg atgtgggaga gtacgttcga
     1201 tgtgatttct gaccccgtgc tcatcatcaa ccggaatttt gagatcaagc gggcgaacaa
     1261 ggccctggct agggcctgtg gtgtggatat caaggaagtg attgcgaaga agtgttacaa
     1321 gatttttgcc ggccttgagg ccccttgcg
//
LOCUS       DATDUN010000578         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_110080, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000578 DATDUN010000000
VERSION     DATDUN010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110080"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VJC18_08125"
     CDS             <1..1313
                     /locus_tag="VJC18_08125"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HLD45386.1"
                     /translation="SFLNIRFLASNVTTLTPSLGLICLCLLLGATGKSAQIPLTIWLP
                     DAMAGPTPVSALIHAATMVTAGVYLIARLHFLFILSPLVMTIMATIGVVTAFLAALLA
                     LTQYDIKKILAYSTISQLGLMFLALGVGAFSSALFHVFTHAFFKAGLFLGAGIIIQAL
                     HHEQDIRHMGGLLKKFPVVGIAFFLCLLSLAGIPPFAGFFSKDEILYFSYALSPYRFH
                     YYLALITAGLTALYSMRLFARAFLGQPHSPPGTTMTATSPVMTGSLIVLTIFSTLAGF
                     VGLPRAWGNHLLGAWLTDWETTTPHHMADAFFQEVVIIIFATLLSLSLMVLVYRLYSR
                     NEAVMAPLKKTLGILYRFIHERFWVDELIEGALIRPLHRFTNGFIFRLVDQKIIDGLA
                     VNGVTNTSRLFARLLALMHSGVIAHYALYLLVALGLFFVLLVLV"
     gene            1319..2842
                     /locus_tag="VJC18_08130"
     CDS             1319..2842
                     /locus_tag="VJC18_08130"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLD45387.1"
                     /translation="MIHEFSQTLSLLINNPHLTMLIFLPFVGAVVMGFIPSDQKNTLK
                     LTALFISLTEALAGVLVSLQFKNTGVIDQFLINLPWIDSFNAHYLLGVDGVSLMLILL
                     TTFTLPLALIGTWNSIQHSFKTYFVCLLLLETGLVGTFASLDLFLFYVFWELTLIPMY
                     FIIGIWGGPQRVYATLKFVIYTMAGSVLLLIAILYLYFHAGNTFNLLELYNYRLAFSA
                     QCFVFAFLAIAFAVKIPLFPLHTWLPDAHVQAPTMGSVILAGILLKMGTYGFFRFGIP
                     LCPDAIQYFQDTLMLIAMISALYGALVCLAQTDIKKVIAYSSVSHMGIVMLGLFSLTT
                     TGMTGGLYQMFNHAVSTGSLFLLAGMLYERSHTLAIPDHSGLAKSMPLLAFFFILSSF
                     SAMAVPLTGGFVGEFLSLLGSFQVKPVLATLATISLVISAVYMLWLIERVFLGSPKRE
                     AQDLQDLNSREIFVMIVLTVLIFWMGVFPKDFLQTIERSTETILYKLDDERTTVYHD"
     gene            2835..>3094
                     /locus_tag="VJC18_08135"
     CDS             2835..>3094
                     /locus_tag="VJC18_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45388.1"
                     /translation="MISLADHLLDALSAGLTLWVLLAGALICLLLDALWPRKMTLLVY
                     SVAILSLLLSLYICFLQWLNLATWPQQHWLVMNFKTLFFIAL"
BASE COUNT          593 a          870 c          712 g          919 t
ORIGIN      
        1 cctcctttct caatattcgt ttcctcgcca gcaacgtgac cacactgacc ccctccctcg
       61 gactgatctg cttgtgtctg ctgctgggag ccacgggaaa atctgcacaa atccccctca
      121 ctatctggtt gcccgatgcc atggccggcc ccactcccgt ctctgccctc atccacgccg
      181 ccaccatggt gaccgccggc gtgtatctga ttgcgcgtct gcattttctt tttatcctct
      241 caccactcgt catgacgatc atggccacca tcggtgtggt cacagcgttc ctggcggcac
      301 tcctggctct gacccaatac gacattaaaa aaattctcgc ctactccacg atcagtcaac
      361 tgggattgat gtttctggcc ctcggtgtgg gcgctttttc gagtgccttg ttccacgttt
      421 tcacccacgc ctttttcaag gccggtcttt ttctgggagc cggcatcatc attcaggccc
      481 tgcatcacga acaggatatt cgtcacatgg gaggtttgct caaaaaattc cccgtggtgg
      541 gaatcgcctt tttcctgtgc ctcctgtccc tcgctggcat tcctcctttt gccggttttt
      601 tcagcaaaga cgaaatcctt tatttctcct acgccctgtc accctaccgt tttcattatt
      661 acctggcact catcacagcg ggtctcacag ccctgtacag catgcgcctg ttcgccaggg
      721 cctttctggg acaacctcat tccccacccg gaaccaccat gactgcgacg agccccgtca
      781 tgacgggttc cctgatcgtc ttgaccatct tttcgaccct ggcaggtttt gtcggtctgc
      841 cgcgtgcatg gggaaatcat ctgctgggag cttggctcac cgattgggaa accacgaccc
      901 ctcaccacat ggcagacgcg ttttttcagg aagtggttat tatcatcttt gccaccttgc
      961 tgagcctctc tctgatggtg ttggtgtatc gcttgtattc aagaaatgag gccgtcatgg
     1021 ccccgctcaa gaaaacgctg ggaatcctct accggttcat tcacgaacgc ttttgggtag
     1081 acgagctgat cgaaggggcc ctgatcaggc cactgcatcg tttcacgaat gggtttattt
     1141 ttagacttgt cgatcagaag attattgatg gcctcgctgt caacggggta accaacacca
     1201 gtcggttgtt tgcccggttg ctggcgttga tgcattcggg agtgattgcc cattatgcct
     1261 tgtacctgct ggtcgctttg ggattgtttt ttgtactgtt ggttttggtg tagaaaatat
     1321 gattcatgaa ttttcacaaa cactttcgct cctgattaat aacccacacc tgacaatgct
     1381 catttttttg cctttcgtgg gagccgtcgt gatggggttt attccctcag atcaaaaaaa
     1441 caccctcaag ctaacagcgt tgttcatttc tctgactgag gccctggcag gggtgctggt
     1501 cagtctgcag tttaaaaaca ccggggtgat cgaccaattt ttgatcaacc ttccctggat
     1561 tgattcgttc aacgcccatt acctgctggg cgttgacggt gtcagtctca tgctgatatt
     1621 gctcaccacc ttcaccctac cactggccct gatcgggacc tggaacagca ttcaacacag
     1681 tttcaaaacc tatttcgtct gtctgttgct gctcgaaaca ggtttggtgg gaacctttgc
     1741 ctctctggat ctgtttctgt tctatgtttt ttgggaactc actctcatcc ccatgtattt
     1801 catcattggg atttggggag gacctcagcg cgtgtacgcc acattgaaat tcgtcatcta
     1861 cacgatggct ggctctgtgc tgctgttgat tgccatactg tatctttatt ttcatgctgg
     1921 caacaccttc aacctgctcg aattgtataa ttaccgtttg gccttttccg cacagtgttt
     1981 cgtctttgcc ttcctggcca tcgctttcgc tgtcaagatt cccttgtttc ctctgcacac
     2041 ttggcttcct gacgcccacg tgcaggcccc cacgatgggt tctgtgatcc tcgcgggcat
     2101 tctgcttaaa atgggaacct atggtttttt cagatttggg atcccactct gtcctgacgc
     2161 catccagtac tttcaggaca ccctcatgct catcgctatg atcagcgcac tctatggcgc
     2221 cctggtttgc ctcgcacaaa cagacatcaa gaaggtcatt gcgtactcat ccgtatctca
     2281 catggggatc gtgatgttgg gtttgttctc gctcaccaca acagggatga caggcggatt
     2341 gtatcagatg ttcaaccacg ctgtgtcgac gggatccttg ttcttgctgg ccggcatgct
     2401 ctacgaaaga tctcacacac tggccatccc cgatcactcg ggactggcca aatccatgcc
     2461 actactcgcc ttctttttca ttttgagttc attctctgcc atggcggtgc ccctgacggg
     2521 cggtttcgtg ggagagtttt tgtcactctt gggaagcttt caggtcaaac ccgtattggc
     2581 aacattggcc acgattagcc tggtgatcag cgccgtctat atgctgtggc tgattgaaag
     2641 ggtctttttg ggatcgccca aaagggaagc tcaagacctg caagacctga acagtcgaga
     2701 aattttcgtg atgatcgtgt tgacagtttt gatcttctgg atgggtgttt ttccaaaaga
     2761 ctttttgcaa accattgaac gcagcacaga aaccatcctg tataaactcg atgacgaaag
     2821 gacgaccgtg tatcatgatt agcctcgcgg atcatttgct cgacgctttg tctgccggtc
     2881 tcaccctctg ggtattgctt gctggtgccc tcatttgtct gctgctcgat gccctctggc
     2941 cccgcaagat gacccttctg gtgtactccg tggcgatcct cagcctgctt ttgagcctgt
     3001 acatctgttt tctacagtgg ctaaacttgg cgacctggcc acaacaacac tggttggtga
     3061 tgaatttcaa aactcttttt ttcatcgctc tgat
//
LOCUS       DATDUN010000579         3914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_442432, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000579 DATDUN010000000
VERSION     DATDUN010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442432"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VJC18_08140"
     CDS             <1..423
                     /locus_tag="VJC18_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45389.1"
                     /translation="VLVTASAFAGFPKINTGNSTMNKVVNTGVSTAVNTTVASDINKA
                     IKSYNCAFKNSTTTTDTTCDMSQVINTIKSKKSALETLGLAKVYVHVKASGNDTTAYK
                     RSNFIRDRLYGEMNWYRYNVSSSTDNSNNLAIWVEVQD"
     gene            575..1612
                     /locus_tag="VJC18_08145"
     CDS             575..1612
                     /locus_tag="VJC18_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HLD45390.1"
                     /translation="MIHKRVGILTGGGDCPGLNAVIRSVVRKGIDDYKYEFLGILQGW
                     KGMIEGKTRSLGLNDISGILHSGGTILGTSRTNPSKSAEDMETVITNYRRMGLNALIA
                     IGGDDTLGVAAKMSQNGLNVVGVPKTIDNDLSGTDQTFGFDTAVSVATEAIDRLHSTA
                     ESHQRVMVVEVMGRHAGWIAAHAGIASGADIVLVPEFKFSLDDIVKVLDKRKKRGRVA
                     TIIVVAEGFSLPDSDLITQDETVDAFGHKKLGGIGNLLGEMIENKTGLETRVSVLGHI
                     QRGGTPTAFDRILATRFGIKAIELVHADIYGNMVALIGSKIEAVDLQQAAQKNRLLNE
                     EIYNIAKVFFG"
     gene            complement(1693..2568)
                     /locus_tag="VJC18_08150"
     CDS             complement(1693..2568)
                     /locus_tag="VJC18_08150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLD45391.1"
                     /translation="MKDWRIRYVVYGLAVFFVSVCFSHTVSAQSSDPELDAQEDETAI
                     VSPADEAGYYGPRIKVNIPARQLFLYDADDKLLKVYDIAVGKSIFPTPVGKRWIDQIV
                     WNPWWIPPNSYWAKDDVPTPPGPTNPLGPVKMKMGTIMFHGNNKDSTIGVPASHGCMR
                     MHNDQAAELARYLQESVTDLTDSNLFKEYKKNSKRSYYVNLTTRVPVEIVYELVEIRH
                     NTLFVYQDVYSRVANKASLVEKKLLERGYDLEDVDIEFISKQLKITKNSDLSFDLPRL
                     MVRKRGNDLSQLAQN"
     gene            complement(2784..>3914)
                     /gene="glmU"
                     /locus_tag="VJC18_08155"
     CDS             complement(2784..>3914)
                     /gene="glmU"
                     /locus_tag="VJC18_08155"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HLD45392.1"
                     /translation="ALASIKNKEGALLVINGDMPFISTKTLRQHLHHHKKQGSVATLL
                     SRVVEDPVAFGRIVRDAHGRFMGVVESRDASSAIKQIREINVGSYVFELSFLHRHINQ
                     LNTSNRQKEYYLPDLFRMAKNNGLVAEAFILRDAVAALGVNSQEDLSTINQIFYERQR
                     RCLMSEGVSLVGKEIYIDFGVRVAKGTVIEAPCYLKGNSSIGEGVTIETGCVIKSSKI
                     AAGTLIKAYSYLDQARVGNHCQVGPFAHLRPKASLRDKAKVGNFVEVKKSVIGQGSKV
                     SHLSYIGDAVIGKKVNVGAGTITCNYDGYHKYKTVLDDEVFIGSDTQLVAPVRVGRGA
                     VVGAGTTVVKNVAADSLVISRCEQKEILGWAVGYRKRNSRSN"
BASE COUNT          989 a         1029 c          902 g          994 t
ORIGIN      
        1 gtgttagtca ccgcgtcagc cttcgccggc tttcccaaga tcaacacagg caacagcacc
       61 atgaacaagg tcgtcaacac gggcgtgagc acggccgtga acacaacagt ggccagcgat
      121 atcaacaagg ccatcaaatc ctacaactgt gccttcaaga acagcacaac aaccaccgac
      181 accacctgtg acatgagcca ggtcatcaac acgatcaaat ccaaaaagag cgccttggaa
      241 accctgggtt tggccaaggt ttatgttcac gttaaagcca gtggcaatga cactacagct
      301 tacaaacgtt ccaatttcat cagagaccgc ctgtacggtg agatgaactg gtatcgttac
      361 aacgtcagct ccagcacaga caactcaaat aacctcgcca tctgggtcga agttcaggat
      421 taaagcaaaa acgatcatat ctattttatt aaaaaacctt cctgctggca ctcccagcac
      481 gaaggttttt ttattttttt tgcgacgatt gcattatcat aaatctcgta gtatcatgag
      541 agtcggttat ttagtatttt tctcgggagg gcctatgata cataagagag tcggaatttt
      601 gacaggtggg ggtgattgcc cgggtctcaa cgcggtgatt cgttcggttg tgaggaaggg
      661 catcgatgat tacaaatacg aatttttggg tattttgcaa ggctggaaag gcatgattga
      721 gggcaagacc cgttcactgg gcctcaacga catttccggg atcctgcact ctggtgggac
      781 tattctggga acatcccgca caaacccttc caaatctgcc gaggatatgg agactgtcat
      841 caccaattac cgacggatgg gactcaacgc cctcatcgcc atcggtggtg acgacacctt
      901 gggcgtggcg gccaagatga gtcaaaacgg tttgaacgtg gtcggcgtcc ccaagaccat
      961 cgacaacgat ttatcgggaa cagatcaaac ctttggtttt gacacggcgg tctcagtcgc
     1021 taccgaggcc atcgatcgtc tgcattccac ggccgaatcg caccagcgcg tgatggtggt
     1081 cgaggtgatg gggcgtcacg ctggttggat tgccgcacac gcaggcatcg cctccggggc
     1141 cgatattgtt ttggtgccgg aatttaaatt ttccctggac gatatcgtca aggtgctgga
     1201 caaacgcaaa aaacgcggcc gcgtggccac catcatcgtg gtggctgagg gattcagtct
     1261 tcccgattcc gatctgatca ctcaggacga gactgtggac gcctttggtc ataaaaaact
     1321 ggggggcatc ggcaacctac ttggcgaaat gatcgaaaac aaaaccggtt tagagacccg
     1381 cgtctccgta ctgggtcaca ttcagagagg tggcacgccc acagccttcg accggattct
     1441 cgccacacgc tttggcatca aagccattga actcgtgcat gccgatattt acggcaacat
     1501 ggttgccttg atcggatcga aaattgaggc tgttgatctg caacaggccg cacaaaaaaa
     1561 ccgtcttctc aatgaagaaa tctacaacat cgccaaggtg ttttttggct gatgataact
     1621 gtctgtctgg caacaaaaaa accgactgtg caagggtcgg ttttttttgg gggcaactct
     1681 gaatcgcagg gtttaattct gcgccagctg actcagatca tttccacgtt tccggaccat
     1741 caaccgcggc aaatcaaaag acagatcaga atttttggtg atcttcagtt gtttgctgat
     1801 gaattcaatg tccacgtctt ccagatcata accccgttcc agtagttttt tctctacaag
     1861 cgacgccttg ttggcaaccc ggctgtaaac gtcctgatac acaaacaagg tgttgtgcct
     1921 gatctcaacc agctcataca caatttccac agggacccgg gtggtgagat tgacataata
     1981 actgcgtttg ctgtttttct tgtattcctt aaacaagttg ctgtctgtca agtcagtcac
     2041 cgattcctgc aggtaacgag ccaactccgc cgcctgatcg ttgtgcatgc gcatgcaacc
     2101 atggctggcg ggaactccga tggttgaatc cttattgttt ccatgaaaca tgatcgtgcc
     2161 catcttcatc ttgacaggac caagaggatt tgtggggcct ggcggggtcg gcacatcgtc
     2221 cttggcccaa taactattgg ggggaatcca ccacgggttc caaacaatct ggtcgatcca
     2281 tcgcttccct acgggtgttg gaaaaatact cttgcccacc gcgatgtcat acaccttaag
     2341 cagtttgtca tccgcatcat agagaaagag ctgtctggca ggtatattga ccttgatccg
     2401 gggaccataa taccctgcct catcagcagg agatacaatg gcagtctcat cttcctgagc
     2461 atccagctca gggtcactgc tttgagctga cactgtgtgt gaaaaacaaa cagagacgaa
     2521 aaacacggca agaccataca caacgtatcg aatacgccaa tctttcatag ttgccccctc
     2581 atgttgcgtt gaccagcgtt gcctgtgcag gtcacaaaag tggccgtaca ccaggcacac
     2641 aattcattat atccgaaatt tcaatggttt gactatggtt tttttggagt taatattttg
     2701 acgatccatc taagtggctt ttgttattgt ttttctgggg attataagaa ttacgtcaag
     2761 gaattgactg ggatttttga atgctaattt gatctggaat ttctttttcg gtaaccgaca
     2821 gcccacccga gaatctcttt ctgctcacag cgtgaaatca ccaggctatc cgcggcaaca
     2881 ttcttgacca ctgttgtgcc cgctcccacc accgcgccgc gaccaacccg cacaggggcc
     2941 accagctgtg tatctgatcc aatgaagact tcatcgtcca gcaccgtttt gtatttgtga
     3001 taaccgtcat aattgcaggt gatcgttccc gcgcccacgt taaccttttt tccaatcacg
     3061 gcatcaccaa tgtaactcaa atggctcacc ttgctgcctt gaccgatcac agattttttg
     3121 acctccacga aattacccac cttggccttg tcccgcaaac tcgctttggg cctcaaatgc
     3181 gcaaacggtc ccacctgaca atgattaccc acacgggcct gatcaaggta agaatacgct
     3241 ttgatgagtg tgccagctgc tatcttcgaa gattttatca cacagcccgt ttcaatcgtg
     3301 accccctcac caatgcttga attgcccttg aggtaacaag gagcttcgat cacggtcccc
     3361 ttggccaccc tgactccgaa atcaatataa atttctttgc ccaccagaga cacaccctcg
     3421 ctcatcagac aacgacgctg acgctcataa aatatctggt tgatcgtgct caggtcttcc
     3481 tgtgaattga cccccaacgc cgctacagca tccctcaaga taaacgcttc cgccaccaaa
     3541 ccgttattct ttgccattct aaacagatct ggcaaataat attccttttg cctgttgctc
     3601 gtgttgagtt gattgatgtg tcggtgcaaa aaagacagct caaaaacgta tgagcccaca
     3661 ttgatttctc gaatttgttt gatggcagag ctggcatccc gggattccac gacacccata
     3721 aaccgcccgt gggcgtcacg cacaatcctg ccaaacgcga cagggtcctc cacgacgcga
     3781 ctcaaaagag tggccacgct accttgtttt ttgtgatggt gtaaatgttg tctgagtgtt
     3841 tttgtgctga tgaaaggcat gtcaccgttg atcaccaaca gggctccttc tttgttcttg
     3901 atcgaggcca aagc
//
LOCUS       DATDUN010000580         2407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_442435, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000580 DATDUN010000000
VERSION     DATDUN010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442435"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VJC18_08160"
     CDS             <1..1185
                     /locus_tag="VJC18_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45393.1"
                     /translation="PLRKTAPAQIAPPEAHFIDPNCQFEGEKYLEGLRLPAGTSFPLY
                     TNRGGFQDCPGPGIKRAIIVIHGINRTALNYFNYIMEGLPQPPATSAGNDVGGVSVNG
                     EVTNARGVSKVIIKSSSSPPQLPVASPHGATIDTSRVNRANAIVIAPYFAKNNGGDDC
                     DPPVCPGHDDYRPSFISWSVGWKTLAPSDASHPYRLSSAEVVDRIVQTLLDEALYPDL
                     DSIVIVGHSAGGQFVTRYAITNPVHDEMINRGIEVRYIVANPSAYLYLDETRYDQTAE
                     VFAVPQTTCSQYNKYGYGLNSPNQYVTAALSEIVDNARTRPVYLLIGDADNFDEGNLD
                     TSCGAMLQGDHRYERAQWYYRYLGQLFPEGHNTKLLAVPNVGHNTRKMFLSPKGAHLI
                     FE"
     gene            1458..2381
                     /locus_tag="VJC18_08165"
     CDS             1458..2381
                     /locus_tag="VJC18_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD45394.1"
                     /translation="MKNKKMSSPPNVFKYDDYRRYLSDFYEFKKLEMPGRFSYRYLSQ
                     KAGFQTSTFLHRVIIGKRNLTLESIRKLAAIIPLKGRPYQYFETLVMFNQAKEEDERL
                     THFEALKEFKEYIALRLINEDQNMYLYKWYYPVVREMIKWSQFKGDPVWISKQIKPGL
                     TPEQAKEALEVLLQLRLAKVNADGTWEQTYPKLMTKEEGLGHEVLNYHKNAIQLGMVC
                     LKQPAKERHVSAMTISVSPAIFELMRQKIYIFHKEISGLISQKINKNTLKEYNVSEEL
                     MQSQGFFDVSEVCQFNLQMFKVAKLEKTNED"
BASE COUNT          635 a          558 c          621 g          593 t
ORIGIN      
        1 cccctgagga aaaccgcgcc tgcgcagatc gcgcctccgg aagcccattt cattgacccc
       61 aattgtcaat ttgaaggcga aaaatatctt gaaggcttga ggctcccggc gggcacgagt
      121 tttccccttt acaccaacag gggaggtttt caggattgcc ccggaccggg aatcaaacgg
      181 gccatcatag tgatccatgg gataaacagg acggcgctca attatttcaa ctacatcatg
      241 gagggtttgc ctcaaccgcc agcaacgtca gcagggaacg atgttggcgg tgtttcggta
      301 aacggagaag tcaccaatgc acgtggtgtt tcaaaagtca taatcaaatc ctcttcctcg
      361 cccccccagc ttcccgttgc gtcgcctcac ggtgcaacga tcgatacttc gcgtgtgaac
      421 agggccaatg ccattgtgat cgctccgtat tttgcgaaga ataatggggg cgacgattgt
      481 gacccccctg tgtgtcctgg ccatgacgac tatcgtccgt cattcatttc gtggtccgtg
      541 ggatggaaaa ccctggcacc ttctgacgcc agtcacccgt accggttgag ttctgcggag
      601 gtggtggaca ggattgtgca gaccttgctc gatgaggccc tgtaccccga cttggatagc
      661 attgtgattg tgggccattc cgctggcggc cagtttgtga cccgctatgc cattactaat
      721 cccgtgcacg atgagatgat caacaggggg attgaggtca gatatattgt ggccaatccc
      781 tccgcctatt tgtatctgga tgaaacgcgg tatgatcaaa cggcggaggt atttgctgtc
      841 cctcaaacaa cctgttccca atacaacaag tatggttatg gtttgaattc ccccaatcag
      901 tatgtgacag cggccctgtc tgagattgtt gataacgccc ggacacgccc tgtttatctg
      961 ttgattgggg atgccgataa ctttgatgag ggcaatctgg atacatcgtg tggtgccatg
     1021 ttgcagggag accatcgcta tgagcgggca caatggtatt atcgctattt ggggcagtta
     1081 tttcccgagg gacacaatac caaactgctg gctgttccca acgtggggca caatacgcgc
     1141 aaaatgtttc tgagcccaaa gggggcacac ttgatctttg aatagccatt atggcgcttt
     1201 ctggtgagtc ctcaaggaca cggaatttgt aaaaaccgtg tccttgagga cacggatcag
     1261 gcgacgtgtt ttttaggatg agttctaatg cgctgatatg gtttgaaaat actgcgttgt
     1321 ttttttctgg tgtgctgaac ttctgctttt gaaaaaatga tttgatcagc aacagagctg
     1381 acgtattcga cgataatagg gtataatcaa agtacccgag cggtcgaata agactgtaaa
     1441 gaaaagggga gtgtatggtg aagaacaaaa aaatgtcgag tcctccaaat gtattcaaat
     1501 atgatgacta ccgccgttat ctgtctgact tttacgaatt caaaaaactc gagatgccgg
     1561 gccggttttc gtatcggtac ctgagtcaga aggccggctt tcagacctcc acctttttgc
     1621 accgcgtgat cattggcaaa cgtaacctta ccctggaaag cattcgcaaa ctggccgcga
     1681 tcattcccct caagggcagg ccttaccagt atttcgaaac cctggtcatg ttcaatcagg
     1741 ccaaggaaga agatgaacgt ctgacgcatt tcgaagccct caaggagttt aaggaataca
     1801 ttgcgctcag gctcatcaac gaagaccaga acatgtatct gtacaaatgg tattatcctg
     1861 tggtgcgcga aatgatcaaa tggtcacagt tcaaaggaga ccctgtttgg atcagcaaac
     1921 aaatcaagcc cggattgacg ccggagcagg ccaaagaggc gttggaggta ctattacaac
     1981 tgaggctggc caaggtcaac gctgatggga cctgggaaca gacgtatccc aaactcatga
     2041 ccaaagaaga agggttgggg catgaggtgc tcaattatca caagaacgcc attcagttgg
     2101 ggatggtctg tctcaagcaa cccgcaaagg aaaggcatgt gagcgccatg accatctctg
     2161 tgtcgccagc gatattcgaa ctgatgagac agaaaattta tatttttcac aaggaaatat
     2221 cagggctgat ttcgcaaaaa attaacaaaa atacgctcaa ggaatacaat gtgtccgagg
     2281 agctgatgca gtcgcagggt ttttttgatg tctcggaagt gtgtcaattc aacctgcaga
     2341 tgttcaaggt tgccaaactg gagaaaacca atgaagacta ataccagaat actgatgctg
     2401 ggtttgt
//
LOCUS       DATDUN010000581         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_151389, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000581 DATDUN010000000
VERSION     DATDUN010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151389"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VJC18_08170"
     CDS             complement(<1..268)
                     /locus_tag="VJC18_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HLD45395.1"
                     /translation="MHTMLSELQIDALKEVSSIGAGNAATALSQMIKKRIVINVPQVQ
                     FSTIDEATRVFGPAETIVSAVCLRVLGDVTGIILITFGKAEATRL"
     gene            complement(314..919)
                     /locus_tag="VJC18_08175"
     CDS             complement(314..919)
                     /locus_tag="VJC18_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HLD45396.1"
                     /translation="MKELTNLQLDALREAGNIGAGHAAIALSQMMNKKVMMSVVRTEI
                     VPSNVFLKGFVEEHDPEVAGVYLITLGDTQGAAIFMFQKAAALNLCDLLLTRQRGSTT
                     FVDEQAQSALKELGSILTGSFFSVLSDMLHLNFFHKTPLYAEDDAAVILYGICDSVFG
                     DRQERLCVTTEFVESNSRIKGSFVFIPTVETMQKFLTQLQV"
     gene            complement(941..>2260)
                     /locus_tag="VJC18_08180"
     CDS             complement(941..>2260)
                     /locus_tag="VJC18_08180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HLD45397.1"
                     /translation="CVFITRESSEKVSQLVNEVLEVKGVQIVLIPADPAWNVLSCGDA
                     ADSDSRESPESFHEHIKKLQSVRVDVLVLDKLMNLVEELTICKLRFADLNMRETSMGL
                     KAAVEQMGRLTDDLQNEVMKARLVPVGQIFERFPRFVRDMAKAENKKVRLDMVGADIE
                     LDRTVLDEMGEPLIHLIRNAVDHGIESPAERKTKNKSEDGTITLSARREKNSVFIEVS
                     DDGKGISVDLVKKQALAKGLVTHEVLQQMNDHEVLLLTTQPGFSTKEAITRVSGRGVG
                     LDVARTKMEKLGGSLLIQSQRDEGTKFSMRLPLTTAVIKALLVKSQGRTYAMPVANII
                     ELVIVKEENINTIENRETFFHRDRVLPLVRMDWESHAYEARNALNVVVVETGNNPFGI
                     VVDSLVSQQDIVVKQLARELRGVRGIAGATILGDGNVALVLDVAGLV"
BASE COUNT          568 a          643 c          468 g          581 t
ORIGIN      
        1 aaagacgtgt cgcttctgct ttcccaaaag tgatgaggat gatccctgtc acgtcaccca
       61 aaacgcgcag gcacacggca ctcacaattg tttcggctgg tccaaaaaca cgggtagctt
      121 catcaatggt cgaaaactgc acctgcggca cattgatgac aatccttttc ttgatcatct
      181 gcgacaaggc tgtggcagca ttccccgccc cgatactgga cacctctttg agcgcatcaa
      241 tctgcaactc actgagcatt gtatgcataa aacatccttt cacttgaacg tatgagcttg
      301 atgacagtca gtgtcatacc tgaagttgag tcaggaattt ttgcatcgtt tcgacagtgg
      361 ggataaaaac aaaggaaccc ttgatccgac tgtttgactc aacgaattct gtggtcacac
      421 acaatctctc ctgcctgtcc ccaaaaacgc tatcacaaat gccgtacagg ataacagcgg
      481 catcatcctc cgcgtaaaga ggcgtcttgt gaaaaaaatt aagatggagc atgtcagaaa
      541 gcacagaaaa aaatgaaccc gtgaggatac tgccaagctc tttgagcgct gattgagctt
      601 gctcatctac aaaagttgtc gagcccctct ggcgtgtgag cagcaaatca cacaggttta
      661 acgcggcagc cttttgaaac atgaaaatcg cggccccttg ggtatccccc aaggtgatga
      721 gatacacccc cgcgacctca ggatcgtgtt cctccacaaa ccctttcaga aacacgttgc
      781 tggggacaat ttctgttctg accacagaca tcatgacttt cttgttcatc atctgagaaa
      841 gggcaatcgc cgcgtgtccc gctccaatgt ttccagcctc cctcagggca tcgagttgaa
      901 gattggtaag ttctttcatt tgttatcccc aaataaggtg ctaaacaaga cctgccacat
      961 cgagcacaag ggccacattc ccgtcaccaa gaattgtggc acccgcaata cctctgacac
     1021 ccctcaattc cctggccaac tgtttgacca caatgtcctg ttggctcaca agcgaatcga
     1081 ccacgatgcc aaatgggttg ttgccagttt cgacaacaac aacgttaagg gcgtttcgcg
     1141 cttcatacgc atgactctcc cagtccatcc tcaccagagg caacacgcgg tcacggtgaa
     1201 aaaaggtttc cctgttttcg atggtgttaa tattttcttc ttttacaata accaattcga
     1261 taatattggc gacaggcatc gcatacgtac gtccctgcga cttaaccagc agggctttga
     1321 tgaccgccgt tgtgagcgga agcctcatgg aaaactttgt tccttcgtcc cgttgtgatt
     1381 ggatcagcag tgaccctccc agtttttcca tttttgtacg cgccacatcc aggcccacac
     1441 cacgtcctga cactctggtg atcgcctcct tggtgctgaa tcctggctgt gtcgtcagca
     1501 acaggacctc atgatcattc atctgttgca ggacctcatg agtgaccagg cctttggcca
     1561 gggcctgctt tttgaccaaa tcaacgctga tgcctttgcc atcatcactc acctcaatga
     1621 aaacagaatt tttttctcgc ctcgccgaaa gcgtgatcgt cccgtcttca cttttgtttt
     1681 ttgttttgcg ctccgcagga gattcaattc cgtgatcgac ggcatttctg atcagatgga
     1741 tgagaggctc tcccatctca tccaaaactg ttctgtcgag ttcgatgtcg gcaccaacca
     1801 tgtcgagtcg aaccttttta ttctccgcct tcgccatatc cctcacaaat ctgggaaacc
     1861 gctcaaatat ctgccccaca ggaaccagcc tggccttcat cacctcattt tgcaaatcat
     1921 cggttagccg tcccatttgc tctacagctg ctttcaagcc catgctggtt tcgcgcatgt
     1981 tcaaatctgc aaagcgcaat ttacaaatcg tcagctcttc aaccaggttc atgagcttat
     2041 ccaacaccag cacatcaacc cgaacactct gcaatttctt gatgtgttca tgaaatgact
     2101 ctggcgattc cctggaatct gagtctgcgg catcgccaca gctcaggacg ttccaggcag
     2161 gatcagcagg gatcaagaca atctgaaccc ccttgacttc aagaacctca ttgaccagtt
     2221 gactcacctt ctccgaagat tccctggtaa taaacacaca
//
LOCUS       DATDUN010000582         1121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_69678, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000582 DATDUN010000000
VERSION     DATDUN010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69678"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VJC18_08185"
     CDS             complement(<1..629)
                     /locus_tag="VJC18_08185"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HLD45398.1"
                     /translation="MFPKRIIIFANGDLPDPDKTHALLRESDFIIAADGGTRHALALG
                     LTPNIIIGDLDSLNVEQPALSLSKGLTFNAEIIQSPADKNETDLDLAIQHALTLNPEQ
                     IIIAAALGGRLDQTLGNIALVSNVQRSTSNVRLDDGIEEVFFCHEQTQIKGASGDIVS
                     LIPWQGEVTGIVTTGLKWPLQNEILYPQKTRGISNEMMNDTATIQIKSGL"
     regulatory      complement(654..762)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(837..>1121)
                     /locus_tag="VJC18_08190"
     CDS             complement(837..>1121)
                     /locus_tag="VJC18_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLD45399.1"
                     /translation="GLKAKNIFPEIRNLGVPSLLVYGSNDPAVAIPTQEKTDALPNHM
                     HQVNLEGAGHFPMIDNPTQFNRLMTDFLALDSGASPRELQMKEEWKRRVR"
BASE COUNT          247 a          249 c          313 g          312 t
ORIGIN      
        1 agtccagatt tgatttggat cgttgctgta tcattcatca tttcgttgct gatgccgcgt
       61 gttttttgcg gatataaaat ttcgttttgc aaaggccatt tgagacctgt tgtgacgatg
      121 cctgtgactt caccttgcca gggaatcaat gagacaatgt caccgcttgc gcctttaatt
      181 tgggtttgtt catggcagaa aaagacttct tcaatgccgt cgtcgagtcg aacgttggac
      241 gttgaacgtt gaacgttact tacaagggcg atattgccga gggtttggtc gaggcgtcca
      301 ccaagcgcgg cggcgataat gatctgctca ggatttaatg tcagcgcatg ttgaatggcg
      361 agatcaaggt cggtttcgtt tttgtctgcg ggggattgaa tgatctctgc gttgaacgtt
      421 aaacccttcg acaggctcag ggccggctgt tcaacgttca acgagtccaa atcaccaatg
      481 atgatattgg gcgttagccc gagcgcgagg gcatgacgtg tgccaccgtc tgctgcaatg
      541 atgaagtcgc tctcgcgcag gagagcgtgg gttttatccg ggtcgggcag gtcgccgttg
      601 gcaaagatga tgatgcgttt tgggaacaaa aatcctccga tggcggagga tggctgtgag
      661 tgaatctaga gtccctccgc tggtattaac cagatcaggt aatgcgggtc gagcgatata
      721 cgctcctctc ggcctgtcaa gcaggctccc cgttcagttg tcttgtgtca tcagtatatg
      781 actcagccct gggaatgtca acataaaaat tgaacgagat gatatttcgt tctgggctat
      841 cgtactctgc gcttccactc ttccttcatc tgcagctcgc gtggactcgc gcctgaatca
      901 agcgcgagaa agtcggtcat cagacggttg aattgggtgg ggttatctat cattggaaaa
      961 tggccagcgc cttcaagatt aacctgatgc atgtgatttg ggagcgcatc tgttttttcc
     1021 tgcgtgggta tcgcaacagc aggatcgttt gatccataga caagcaaact gggaacgcca
     1081 aggttgcgaa tctcggggaa tatatttttc gccttgagtc c
//
LOCUS       DATDUN010000583         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_399403, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000583 DATDUN010000000
VERSION     DATDUN010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399403"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1178
                     /locus_tag="VJC18_08195"
     CDS             195..1178
                     /locus_tag="VJC18_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLD45400.1"
                     /translation="MARPLRIEYDGAWYHVMNRGLERRNIFREPKDYDHFISLLKEIT
                     EIFSIEIHAFSLMPNHYHLLIHTPKMGLSRAMRHLSGVYTQCFNKRYKRDGVLFRGRY
                     KACLVDSNEYLTELVRYIHLNPVKAGLCQHPKEHLWTSHLYYLTDCKGYDWLFRDILR
                     EYGKTTRVARQNFDLMVRGNLSEEIIQAIEQPKNGIIGGDFFVSWVNENFVESRHKKA
                     KEISRKEKELQSSVTGRDIIENIQHCYDVNLAELRSTIRGQRNEARSLAIYLMRHRLG
                     YSLKTISRWFRVENEYVIAKTLSRFKKDLNEKTCLKRMREIEHAVLRKASP"
     gene            complement(1096..1863)
                     /locus_tag="VJC18_08200"
     CDS             complement(1096..1863)
                     /locus_tag="VJC18_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45401.1"
                     /translation="MFLSVCNRGMTGPFSVSPHFLLLPDADPTLDPDRHPTIDVYSGQ
                     TIPSTHCYVMGSDGHDGGSCFSAGEMLSPHAQIRRELEAITSDKVRESTGVFRKVTSV
                     FCHASFKDGDVPLLPTDKDSPYDQFLSRHERALTREEKERELTRYKARLFAYFDSLLP
                     VLELDVHMNDSELKERVRTFFTELRAEIACVAQQDRQAVAVMLSLVLHVKEQIQRLGR
                     LRIEEWGQVLTRDLLFSKRHVLFPSFVSNMSSHSGLF"
     gene            complement(1898..>2693)
                     /locus_tag="VJC18_08205"
     CDS             complement(1898..>2693)
                     /locus_tag="VJC18_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HLD45402.1"
                     /translation="PIFSATFSHANMRGVVVHHGDLGGSLFEEADLTGSEFINTTLTG
                     ANFNKAELTGALMRGIDAVYASFIGAKLEQAVLLDACLAQADFEKANLTEAVLIGAQM
                     PQARLVNADLTDAVLSQANLEHANLEGALLVGAQARGTNFEHAHLKWVDLFCADLMGA
                     NLKYTTFTNVYLTSTDLSDVDLTGADIGGTDFTTAIVADAIFAGVRLTWQNIFGYDIL
                     DSNGKADVDPGTKVVTVMSLLCRLLSGADLSAAILPPEDLRGIKIP"
BASE COUNT          762 a          651 c          638 g          642 t
ORIGIN      
        1 aaaacgaccc ctacgaacaa aaaaaaatca gcgcacgtaa accagagctg actcatatca
       61 atactcagcc tcgtgaatgg tcaatccatc ttctccggct ccaaaacagc gtcgtgccaa
      121 ggcttcatga attgaccctg cgaggggtgg ggtcaggtct tgactaggga catgatacct
      181 gctacagcgc agtaatggca agaccgctaa gaatagaata tgatggtgct tggtatcatg
      241 tgatgaaccg tgggttggag cgtcggaata ttttcagaga acccaaggat tatgatcact
      301 tcatctcgtt gctcaaagag attaccgaaa tattttcaat tgaaatacat gccttttcgc
      361 tcatgcccaa tcattatcat ctgctgattc atactcccaa gatgggatta agcagggcca
      421 tgcgacatct cagtggcgtg tatacacaat gttttaacaa acgctacaaa cgagatggag
      481 tcctgttccg ggggagatac aaagcatgtc ttgtagacag caacgagtat ttaactgagc
      541 tcgttagata cattcatctc aatcctgtaa aagcagggtt gtgccaacac cccaaagagc
      601 atctatggac aagtcatctg tattatttaa cggattgcaa gggatacgat tggctgttta
      661 gagacatatt acgtgaatac ggtaaaacga caagggtcgc acgtcaaaac tttgatttga
      721 tggtaagagg caatttgtcc gaagaaatca ttcaagcaat tgaacaaccc aaaaacggta
      781 ttattggagg ggattttttt gtaagttggg ttaatgagaa ttttgtagaa agtcgtcata
      841 aaaaagcaaa agaaatcagc agaaaagaaa aagagctcca atcatcggta actgggaggg
      901 atataattga gaacattcaa cactgttatg atgtgaattt ggctgagctg aggtcaacca
      961 taagggggca gagaaatgaa gccagatcat tggcgatata cctcatgcga catcgactcg
     1021 ggtattctct gaaaacaata agcaggtggt tcagagtaga aaatgagtat gttatcgcca
     1081 aaacgctaag tcgttttaaa aaagacctga atgagaagac atgtttgaaa cgaatgaggg
     1141 aaatagaaca tgccgttttg agaaaagcaa gtccctagtc aagacctgac cccactcttc
     1201 tatcctcaat cggcccagtc tttgaatctg ctccttgacg tgcagaacta atgagagcat
     1261 cacggcaaca gcttgccggt cttgttgcgc cacacaagca atctcggctc gaagttcggt
     1321 aaaaaatgtg cgaacacgct ctttcaactc actatcattc atgtgcacat ccagttccag
     1381 cacaggaagc aacgagtcaa agtacgcaaa taaacgggcc ttgtatctgg tcagttccct
     1441 ctctttttcc tctcgagtga gtgccctttc gtgtctgctc aagaattgat catatggtga
     1501 gtctttgtcg gtaggtagca ggggaacatc cccatctttg aaactggcgt ggcaaaatac
     1561 ggaggtcacc tttctgaaca ccccggtgct ctcccgcact ttatcggagg tgatggcctc
     1621 aagctcccgg cgtatttgtg cgtgagggct gagcatctcc cctgcagaaa agcaggaacc
     1681 accatcgtgc ccatcggagc ccatcacgta acaatgtgtc gacggtatgg tttgccctga
     1741 atacacatcg atcgtagggt gcctgtcagg atcaagcgtg ggatcagcat cgggcaatag
     1801 aaggaaatgc ggggacacag aaaaggggcc ggtcattccg cggttgcata ctgacagaaa
     1861 catagagcac ttcctttttt gagataggta tgatacccta cggtattttg atgccccgca
     1921 gatcctctgg cggcaagatc gccgcagaca agtccgcgcc agacaataat ctgcacagca
     1981 aactcattac tgtcaccacc ttggtccctg ggtcaacatc ggctttgcca ttgctatcga
     2041 gaatatcata gccaaagata ttctgccagg tcagccgcac acccgcaaaa atggcgtcgg
     2101 cgacaatcgc tgtcgtaaaa tcagtccccc ctatgtctgc ccctgtcagg tccacgtcgg
     2161 acaaatcggt gctggtcaga tagacatttg tgaaggttgt gtatttcaga ttcgccccca
     2221 tgaggtcagc gcaaaagagg tccacccact tgaggtgcgc gtgttcaaaa ttggtccccc
     2281 tcgcctgggc gccaacgagc aaggctccct caagattggc atgttcgaga tttgcctgac
     2341 tcaacacagc atcagtcaga tcggcattga ccaatctggc ctggggcatc tgcgctccga
     2401 tcagcactgc ctcagtcagg tttgcctttt caaaatcggc ctgtgccaaa caggcatcga
     2461 gaagcaccgc ctgctctaat tttgccccga taaacgacgc atagacagca tcgatgcccc
     2521 tcataagcgc ccccgtaagc tctgccttgt tgaaattcgc acctgtcagg gtcgtgttga
     2581 taaattcact gcctgtaaga tcggcctctt cgaataacga tccgcccaga tcaccgtgat
     2641 gcacaacaac cccgcgcata ttggcatgag aaaatgttgc cgagaaaatc ggg
//
LOCUS       DATDUN010000584         3832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_399486, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000584 DATDUN010000000
VERSION     DATDUN010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399486"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VJC18_08210"
     CDS             complement(<1..446)
                     /locus_tag="VJC18_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HLD45403.1"
                     /translation="MTTNIIIVGANGRMGKNVIQAVQADPQLRLVGATDSVHCSALGK
                     DAGANAGGAHLGVIIGSDLDSVFKENFGVVIDFSSPVSTIHHVGICQKHKAPLIVGTT
                     GLNAGQEQIILETGKFIPIVRAPNMSTGVNVLFSILAQVAEVLNED"
     gene            complement(448..1359)
                     /gene="dapA"
                     /locus_tag="VJC18_08215"
     CDS             complement(448..1359)
                     /gene="dapA"
                     /locus_tag="VJC18_08215"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HLD45404.1"
                     /translation="MLLFQGSMVAIVTPFKGGQIDESALRGLIDFQLGAGTSCVVPCG
                     TTGESATLSEQEFERVVAITVEHVAGRAKVLAGAGSNDTQRAIKRHKFCKAAGAQGSL
                     QITPYYNKPPQEGLYQHFQAIAKSADLPIVLYNVPGRTAVNMLPQTTVRLSKIDTIVG
                     IKEASGNLDQAKTIIQETDDFFALLSGEDALTCDLYRLGARGAISVTCNITPRECARQ
                     YRAVQEGDFDTAQKIHEDLLALHKILFVETNPIPVKAVLAMMGFIQEEYRLPLVCLSP
                     ENRDKLKTVLADKGFSITSRQHRKERV"
     gene            complement(1380..2273)
                     /gene="dapF"
                     /locus_tag="VJC18_08220"
     CDS             complement(1380..2273)
                     /gene="dapF"
                     /locus_tag="VJC18_08220"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HLD45405.1"
                     /translation="MSFTKMHGTGNDFIILNGISQTLPPYLGQLSKKICDRRFGIGAD
                     QVLVVQPSTAADFKMLIFNADGGEVEMCGNGIRCFAKYVRDQGLTQQTELVVETAAGL
                     IKPEIVLDHPRTIPSTLWVKVDMGEPQSIHAKTSPAVVQLPPWNIEVPITVDQVPASW
                     RSDDQRRFFINEISMGNPHCVIFVHDVEHYPVERVGPYFENHPRFPNRTNVEFVQIID
                     RNRIKQRTWERGVGETLACGTGACASVVASVLNNMTDKNVTVSLKGGDLEIKWSQRTG
                     HVIKTGPATTVFEGEMIMGNG"
     gene            complement(2328..3335)
                     /gene="hpnH"
                     /locus_tag="VJC18_08225"
     CDS             complement(2328..3335)
                     /gene="hpnH"
                     /locus_tag="VJC18_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HLD45406.1"
                     /translation="MSIPLSQGLRVFSYIVGQKLRGNNKYPLVLMLEPLFRCNLSCAG
                     CGKIQYSEEILNKQLSVKDCLSAADECGAPVVSLPGGEPLLHPEIGEIVAGLIKQKRY
                     IYLCTNAILLKRKLSLFQPSKYLTFSVHLDGLKEEHDRAVCRDGVFDQAVAGIKEALA
                     KGFRVTTNTTLFDWVDPERVRSFFDFAMGLGVEGMVLSPAYPYVKAKDQDGFLHRTKS
                     REYFRKVLSDKKAHWQFNQSPFYLKFLTGEKDYSCTPWGCPTRNVFGWQRPCYLLEEG
                     FAQTYQELVNSTDWEKYGHTTNPKCADCMVSCGYEPSAVIDGFSSLKQFANMAREFHA
                     G"
     gene            complement(3361..>3832)
                     /locus_tag="VJC18_08230"
     CDS             complement(3361..>3832)
                     /locus_tag="VJC18_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45407.1"
                     /translation="IYINCILVTVSSFISQSMVQFVTHKPAQVVAGLPGYLCGIIVLP
                     FVFLGITYVLAGIYHLLFTLVGGSKKDYRTTATVYGLGTLIGIFGIVPVLGTIVGIVY
                     HLLVNIGGQAKAHEISTTRAACSVLLLACGCCCLLGVFAFTFSAFIATIASSFK"
BASE COUNT         1001 a         1044 c          863 g          924 t
ORIGIN      
        1 aaatcttcgt tcagcacctc ggccacctgc gccaagatcg aaaacaaaac attcacgcct
       61 gtgctcatat tgggcgcccg aacaatgggg atgaatttgc ctgtttctaa aatgatctgc
      121 tcttgcccgg cattcaagcc cgttgtccca acgatcaggg gcgctttatg cttttggcaa
      181 atgccgacat gatgtatcgt gctgacgggg gaagaaaagt caatcaccac accaaagttc
      241 tccttgaaaa cactgtcaag atctgacccg atgatcaccc ccagatgtgc tccaccagca
      301 ttcgcgccgg catctttgcc caacgcagaa caatgcacag aatcagtcgc ccccaccagc
      361 ctgagctggg gatctgcctg aacggcctgg atcacattct tacccatgcg tccattggcc
      421 cccacaatga tgatgttcgt tgtcatttca caccctttcc tttcggtgtt gcctgctggt
      481 tatcgaaaaa cctttatcgg ccaaaacggt ctttagttta tcgcgattct ctgggctcaa
      541 acacaccagc ggcaggcgat attcttcttg tatgaacccc atcatggcca agactgcttt
      601 gactggaatc ggattcgttt cgacaaatag gattttgtgc aaagccaaca agtcttcgtg
      661 aattttttgg gccgtgtcaa aatcaccttc ctgaacagct cggtactgcc tcgcacattc
      721 ccttggtgtg atgttacagg tcaccgaaat cgcccctcgt gcccccaaac gatagagatc
      781 acaagtcaaa gcatcttcgc cggacagcaa ggcaaaaaaa tcatccgttt cctgtataat
      841 cgttttggct tggtccagat tcccactggc ctctttgatc ccaacgatcg tatcgatctt
      901 cgacaacctg acagtcgtct gcggcaacat attcaccgcg gttctgcccg gcacattgta
      961 caacacgatg ggcaggtccg cagattttgc gatggcctga aagtgctgat acagcccctc
     1021 ttgcgggggt ttgttgtaat aaggtgtgat ttgcaatgat ccctgcgcac cagcggcttt
     1081 acaaaatttg tgcctcttga tcgccctttg tgtgtcgttg gaaccagccc ccgccaaaac
     1141 ctttgccctg cccgcaacat gttcgacggt aatcgcgaca accctttcga actcctgttc
     1201 ggacaaggtt gccgattccc ccgtggtgcc acaaggaaca acacacgagg ttcccgcccc
     1261 aagctgaaaa tcgatcaggc cccgcagggc cgactcatca atttgtccac ccttgaaggg
     1321 tgttacaatt gctaccatgg aaccttgaaa taataacata acccttctcc cattacccat
     1381 tacccattac ccattatcat ctccccctca aacacagtgg tcgccgggcc tgttttgatg
     1441 acgtgtccgg tcctctgcga ccatttgatt tccagatcac ctcctttgag tgaaacggtc
     1501 acgtttttat ctgtcatgtt gttgagcaca ctggccacca cagaggcaca agcacctgtc
     1561 ccacaagcca gagtctctcc cacgccgcgt tcccacgtac gttgtttgat gcggttgcgg
     1621 tcgatgattt gaacaaactc aacattcgtg cgattgggaa acctggggtg gttttcaaaa
     1681 taagggccaa cgcgctcaac gggataatgt tccacatcgt gaacaaagat cacgcaatgc
     1741 ggattgccca tggatatttc attaataaaa aacctgcgct gatcgtctga ccgccaggat
     1801 gccgggactt gatcaacggt aatcggaacc tcaatgttcc agggcggcaa ctggacaaca
     1861 gcaggagacg ttttggcgtg aatggattgg ggttcaccca tgtcgacctt cacccaaagg
     1921 gtgctcggta ttgttcttgg atgatcgaga acaatctctg gtttgatcag tcctgccgct
     1981 gtttcgacca ctaactcagt ttgttgcgtg agtccctggt ctcgaacgta cttggcaaaa
     2041 cagcgaatcc cgttgccgca catttcgacc tcaccaccat cggcgttgaa aatgagcatc
     2101 ttaaaatcgg ccgctgtgct tggttgtacg accaaaacct gatcggcacc aatgccaaac
     2161 cgtcggtcac agattttttt ggacaactga cctaagtaag ggggaagcgt ttgactaatc
     2221 ccattgagaa tgatgaaatc attgcccgtc ccgtgcattt ttgtgaatga aatttgcatc
     2281 gtacccagct aacagaaggt tgcccattgg tcaaataacc cccgttttca ccccgcgtga
     2341 aactcccgcg ccatattggc aaattgtttg agtgaagaaa atccatcgat cacggcactg
     2401 ggctcgtaac cgcaactcac catgcagtcc gcacatttgg gatttgttgt atgcccgtat
     2461 ttttcccagt ccgtgctatt gacaagctcc tgatacgttt gggcaaagcc ttcttccaga
     2521 agataacagg gtctttgcca gccaaatacg ttacgggtgg ggcatcccca aggggtacag
     2581 ctataatctt tctcaccagt caaaaatttg agataaaacg gagactgatt gaattgccag
     2641 tgtgcttttt tatctgacaa aaccttacga aaatattccc gtgattttgt acgatggagg
     2701 aatccgtcct ggtcctttgc cttcacatag ggataggctg gtgacaacac catgccctca
     2761 acaccaagcc ccatggcaaa atcaaaaaaa gaacgaaccc gttccgggtc aacccaatca
     2821 aacagggttg tgttcgtggt cacacgaaac ccttttgcca gggcctcctt aatgccggca
     2881 acagcctgat caaaaacccc atcacgacac acagcccgat catgctcttc cttgagtcca
     2941 tcaagatgca cactgaaggt gaggtactta gagggctgga acagcgataa cttgcgtttg
     3001 agcaagatgg cattggtaca aagataaatg tagcgtttct gtttgatgag ccccgccaca
     3061 atttcgccaa tctctgggtg aagcaggggc tctcctccgg gaagggacac cacgggagca
     3121 ccacattcgt cggcggctga taaacagtcc ttaaccgaaa gttgtttgtt cagaatttct
     3181 tctgaatatt gtattttgcc acaacctgcg cacgacaaat tgcacctgaa gagaggctcc
     3241 agcatcaaaa ccagtgggta tttattattg cccctgagtt tttgcccaac aatgtacgaa
     3301 aaaaccctga ggccttgtga aagaggtatg gacataatag ccagttaaca gaggtgttcg
     3361 ttatttgaat gaggaggcga tcgtggcaat gaaggcgcta aacgtgaagg caaacacccc
     3421 caacagacaa caacagccac acgccaataa caaaacagag cacgccgcac gcgtggtaga
     3481 aatttcgtgc gccttggcct gcccgccaat gttgaccagc aggtgataca caatgccgac
     3541 gatcgttccc aacacaggca cgatgccaaa gataccgatc aacgtcccca aaccatacac
     3601 cgtagccgtc gttctgtaat ctttttttga ccctcccacc agagtgaaca gcaggtgata
     3661 gatgccagcc aagacatagg tgattcccaa aaaaacaaac ggcaaaacaa tgatgccgca
     3721 caaatagccc ggaaggcccg ccacgacctg tgctggtttg tgggtaacaa actgcaccat
     3781 agactgcgaa atgaaagagg aaactgtgac caaaatacaa ttgatataga tg
//
LOCUS       DATDUN010000585         1317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_31178, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000585 DATDUN010000000
VERSION     DATDUN010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31178"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VJC18_08235"
     CDS             complement(<1..841)
                     /locus_tag="VJC18_08235"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HLD45408.1"
                     /translation="MWLIIPGLFILFFLHFSLRSRVNKLQDDFAGLKADLKKSGVKID
                     TPPAQEPLPEHVQELLHTESAGETSHGQAYAQSACTKASTTTEEDVEAILQIETKQEP
                     APPVAPEPVLQAAPQGPLPPSRFDEAVQGVSGFIKDFFTSGNLVLKIGLLLVFVGSIF
                     LVKYSVEQGWFPVELRLLIICALGLALTVVGFRVRETRQNITYLLQGGGIAIFYLTIF
                     AAFKFYQVLPSWLALLILLATVTGASFLAIIQRSLAMAVLSVLGGFLAPIITSTGSNN
                     FTAL"
     gene            1052..1303
                     /locus_tag="VJC18_08240"
     CDS             1052..1303
                     /locus_tag="VJC18_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45409.1"
                     /translation="MNRYFPLVCLVAVGVLLTAGKKKDPKEYVFCPDHSQIETLDYPA
                     GGKQQFCIDKQTGFKKVTAGDCQVRFPTLPDSVWHPAVK"
BASE COUNT          356 a          325 c          329 g          307 t
ORIGIN      
        1 aaagcgcggt aaagttattg gatccggtag acgtgatgat cggggccaaa aaaccaccca
       61 agacactgag cacggccatg gccaacgacc gctggatgat ggcaagaaac gacgcgcccg
      121 tcacagttgc caagagaata aggagggcca gccaagaggg aaggacctga taaaatttga
      181 aggccgcaaa gatggtgaga taaaaaatgg cgatgccgcc gccctgcaaa agatacgtga
      241 tgttctggcg ggtctcgcgc accctgaagc ccacgacggt cagggccagc cccagcgcgc
      301 agataatcaa cagacgcaat tctacgggga accatccctg ttccacagag tatttcacca
      361 aaaaaattga cccaacaaaa accaacagaa gaccgatctt gagcaccaga ttaccagagg
      421 tgaagaagtc cttgataaaa ccggacacgc cctgcacagc ctcatcgaac cgcgatgggg
      481 gcagaggccc ttggggggcc gcctgaagaa caggctctgg cgccaccggg ggcgctggtt
      541 cctgttttgt ctcgatttgc aagatcgctt ctacatcttc ctcggttgtt gttgatgctt
      601 ttgtgcaagc gctctgggca tacgcctgac catgagatgt ttcacccgcg gactctgtat
      661 gcaggagttc ttgaacgtgc tctggcaaag gctcctgtgc aggaggcgtg tctatcttaa
      721 cgccagattt tttcaaatca gccttgaggc ctgcaaaatc gtcttgcaat ttgttgacgc
      781 gggacctcag actaaaatgg agaaaaaaaa gaataaaaag ccctggtatg atcagccaca
      841 tagcaccccc tttttactaa gattgaccgt tgcagaaata tggcccccat agattactac
      901 gaactctctc tgctcaattt atgttttgaa aaattgcttt ttttattgta cccgaagacc
      961 tcttgtctgg caatttctaa gtttgtccga aaccgactgt gccacacctg ttacgcacag
     1021 gctgatcgga tacgttctgt ggggcaacga tatgaataga tactttccat tggtttgttt
     1081 ggtagctgtt ggcgttttgt tgacagccgg aaaaaaaaaa gatcccaagg aatatgtgtt
     1141 ttgccccgat cattcacaga tcgaaacact tgattatcct gctggcggaa aacagcagtt
     1201 ttgtatcgat aaacaaaccg gcttcaaaaa ggtaaccgcg ggtgattgtc aagtccgttt
     1261 tccaaccttg cccgacagcg tgtggcaccc tgccgttaag tgatgcagag cgttttt
//
LOCUS       DATDUN010000586         1921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_361706, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000586 DATDUN010000000
VERSION     DATDUN010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361706"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="VJC18_08245"
     CDS             complement(<1..1553)
                     /locus_tag="VJC18_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLD45410.1"
                     /translation="MTNNVFSFFFAWQNLLLLVLCVPNAFAQTGPSVEVDRIVIVESL
                     QSDPLPSGSIKPSADISLKATIPKTESAADVVSQVAGVHIKRFGGLEDATSISIRGST
                     NAEVNVFLDGVPLDTASADGVGLGQIAATSLSRIEIFKSTSPSELGGSAIGGVINLSS
                     KKAEPGLNQRYGFGFGSFMTYDGLAELSYGGVKNDVVFGFDARRTDGDFMYQDNNGTP
                     LNPADDQEVRRQNSEKQALHPHLNWKHYFNERNEMTLAQHFFRIDAGVSGLGAFQSQT
                     ADKSLTEYLGSVAMKSRGHFGDKSALTHKTYYRLIKSQFSDPNAEIGLGRAQDNDNLT
                     VVIGNRHMWQTDFSKKVAVKKGAEHVYERFAPRDFLAADPVGSTSARHQLNFFVEPHL
                     YLFERKVHLSSQGQSLNVFYDINNDDPSLNDSGTFFSNKIENQFAGQMALIYSPFSAW
                     HVKVSAGREVRLPKFVELFGDQGFVLGNPQLSSEKSFKYDIGFEGAKSFAGFVNKITV
                     GVALFENRI"
     gene            complement(1550..>1921)
                     /locus_tag="VJC18_08250"
     CDS             complement(1550..>1921)
                     /locus_tag="VJC18_08250"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HLD45411.1"
                     /translation="RTREFPAFYSATSGHPLTMWVESAREIAELFHTQMRVGLRQGIV
                     VANPIPKEFEIPKERIDRYLEEAFVEVNEKKILGKNITPYLLRRMSELSQGETLTANI
                     KLLENNAWLASDIAKNLISDL"
BASE COUNT          481 a          528 c          452 g          460 t
ORIGIN      
        1 tcaatgcgat tttcgaacag agcaaccccc acagtgattt tgttgacaaa acctgcaaag
       61 gactttgctc cctcaaaacc aatatcatac ttaaaactct tctcgctgga tagctgtggg
      121 ttgcccaaca cgaagccctg atccccaaac agctcgacaa acttcggcaa cctcacctcg
      181 cgacccgcgc tcactttaac gtgccaggcg gaaaagggcg aataaatcag tgccatttgg
      241 cctgcgaatt ggttttcaat tttgttcgaa aaaaaagttc cgctgtcatt cagggacgga
      301 tcatcgttgt tgatgtcgta aaagacattc aagctctggc cctgcgatga caagtgaact
      361 ttacgctcaa acagatacag atgaggctca acaaaaaaat tgagttggtg ccgcgcgctc
      421 gtactgccca cggggtcagc cgccagaaaa tctctggggg caaacctctc ataaacatgc
      481 tctgccccct ttttcactgc cacctttttt gaaaaatcgg tttgccacat atggcgattg
      541 ccgatcacaa ccgtgaggtt atcattgtct tgcgctctcc ccaaaccaat ctctgcgttg
      601 gggtcggaga actgcgattt gatcaggcga taatatgttt tgtgcgtcag cgcggacttg
      661 tcgccaaaat gtccgcgact cttcattgcc acactgccca aatactcggt gagactttta
      721 tcggctgtct gggattgaaa cgcccccagc cctgagaccc ctgcatcaat ccgaaaaaaa
      781 tgctgagcca gggtcatttc atttcgttcg ttaaaataat gtttccagtt caaatggggg
      841 tgaagggcct gcttttcact gttttggcgt ctgacctcct gatcgtccgc gggattgagc
      901 ggcgtgccat tattatcctg atacataaaa tcgccatcgg tgcggcgcgc atcgaaacca
      961 aacaccacat catttttcac acccccataa ctcagttcgg ccagaccgtc gtacgtcatg
     1021 aagcttccaa aaccaaaacc gtagcgttga ttcaaccccg gctcagcctt cttcgaactg
     1081 agattgatca ccccgccaat ggcgctccca ccaagctcag aaggcgaagt tgatttgaaa
     1141 atctcaatgc gggacaagga ggttgcggca atttgaccca gtcccacacc gtctgctgag
     1201 gcggtgtcca gaggcacgcc gtccaaaaaa acattcacct ccgcgttggt agacccacga
     1261 atcgaaatgg atgtggcatc ctccagcccc ccaaatcgct tgatgtgcac acccgccacc
     1321 tgactcacca catcagcggc agactccgtt ttgggaatcg ttgctttgag ggaaatgtcc
     1381 gcggagggtt tgatcgaccc cgaaggcaaa ggatcagact gcaacgattc aacgatcaca
     1441 attctgtcaa cttcaacaga gggacccgtc tgggcaaatg cgttcgggac acacaaaacc
     1501 agcaacagca ggttctgcca ggcgaaaaaa aaactgaaga cattgttcgt catagatcgg
     1561 aaatgaggtt tttggcgatg tcactggcaa gccacgcgtt gttctcgagc aatttgatgt
     1621 tggctgtgag cgtctccccc tgggatagct ctgacatgcg ccgcaacagg taaggagtga
     1681 tgttctttcc taaaatcttt ttctcattca cttcgacaaa ggcctcttcc agataacgat
     1741 cgatgcgttc cttggggatt tcgaattcct tgggtatggg attggccacc acaatgccct
     1801 gtctgagccc tactctcatc tgggtatgga acagctccgc gatctctctg gccgattcga
     1861 cccacatcgt gagcgggtgt ccactggtag ccgagtaaaa ggcgggaaat tcgcgggtgc
     1921 g
//
LOCUS       DATDUN010000587         2963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_232767, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000587 DATDUN010000000
VERSION     DATDUN010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232767"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..446)
                     /locus_tag="VJC18_08255"
     CDS             complement(21..446)
                     /locus_tag="VJC18_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45412.1"
                     /translation="MITANNINSNRYVTCSSASATDSPLGAYRDLTRPGLYSDDPIIA
                     EHENKFIGGGHHAFKPDFGPLGAFGTGAIYEGSQKIVVRGRNGKPTSWEFYEWRPTGV
                     YTGDGWPVYGWVRSERPEEDIVQIPFVRQIIDDYQTIKK"
     gene            complement(933..1586)
                     /locus_tag="VJC18_08260"
     CDS             complement(933..1586)
                     /locus_tag="VJC18_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLD45413.1"
                     /translation="MPRINRFDYLANDMLLHVRIQINNGEFRFFEERHFELFNRICRM
                     YLKKYPTIKLTDYEWMSNHAHFIFHIDVYTDLPKFMHDFCWRFSFEYNKMQNRKGHLF
                     QQRYRASNVNTDQYALSCQRYVYRNQLRAGMVKELQDTKWSSYHYYAYGQPNDLITPI
                     FTYEQFGNTPEKQQIEFRFFVEKMPEHEETLWRAKLLHPLLKTKKEIVLNYMKKCHV"
     gene            1922..>2963
                     /gene="purB"
                     /locus_tag="VJC18_08265"
     CDS             1922..>2963
                     /gene="purB"
                     /locus_tag="VJC18_08265"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HLD45414.1"
                     /translation="MIERYSRPEMVQIWEPENKFKIWLEIELLALEAWAKEGAVPQKA
                     VAICRKKANFDVARIDELEKTIKHDMIAFLTNVNENIGPEARYLHKGMTSSDVLDTSF
                     AYQLKQAGDILKKDLDQLLKVLKRRAHEHKNTVRMGRSHGVHAEPTTFGLTLAMYYDE
                     FKRARKRLDDATEQVSVGKISGAVGTFAHISPRVEEYVCRKLGLKPAPVSTQIVQRDS
                     HAHFFTVLAVIAASIEKLATEIRHLQRTEVYEAEEYFSPGQKGSSAMPHKRNPVLTEN
                     LCGLARVLRGYAVTAMENVALWHERDISHSSAERIIAPDATVTLDFMLSRLTQVMDKL
                     LVYPDNMMANLNW"
BASE COUNT          837 a          631 c          681 g          814 t
ORIGIN      
        1 gagggattca taggatacta ttatttcttt attgtttgat agtcatctat aatttgacgc
       61 acaaagggta tttgtacaat gtcctcttcc ggacgctccg acctgaccca cccataaaca
      121 ggccagccat caccggtgta cacgccagta ggccgccatt cgtaaaattc ccaactggtt
      181 ggcttgccgt ttctgcccct aacaacaatt ttttgtgagc cctcatatat cgcaccagtg
      241 ccaaaggccc ccaaaggccc aaaatctggt ttaaaggcgt ggtgtccacc accgatgaat
      301 ttgttctcgt gttccgcaat gatgggatca tccgaataca aaccaggtct ggttaggtct
      361 ctgtaagctc ccaaaggact atcggtagct gatgctgacg aacacgtaac atatctgttt
      421 gagttgatat tgttggctgt aatcatctta actcctttca ttgtagtcat aagcaagggt
      481 cgtgccaaaa gaagcaaaat taattatata aaaaacaaca ggttggtgaa gatgtgcttg
      541 tgcgcgtccc acgagtaatc caaaattgtg ggacggtggg atttttttga gacgctgaac
      601 ctctaataac tcgcattggt acttgttgca cagattccca tgtcacccat aaaatgctta
      661 tggaaatcga tagcggtgcc agatacctta tttcgatagc ggtgccagat accttatttc
      721 aaaactgtga gaaggtcgct gtttttgtga taatatattc aaaacggtat tggttattga
      781 agttgaggca ggcctctgag tttgttcaat atatgatcga aagaattttc gttaaacaat
      841 gcaatattcg gactttctct agtcgagtca gaatgtgaat aatatgtgct tggcaatatg
      901 tagaaatcaa acgtgattca attagctatt tttcacacat gacatttttt catgtagttc
      961 aaaacgattt ctttttttgt tttaagaagg ggatgtaata atttggcacg ccacagggtt
     1021 tcttcgtgtt caggcatttt ctctacaaag aatctaaact caatctgttg tttttctggg
     1081 gtattaccga attgttcata ggtaaaaatg ggtgtgatca agtcattcgg ttgcccataa
     1141 gcataatagt gataactact ccattttgtg tcttggagct ccttgaccat tcctgccctc
     1201 aattgattac ggtaaacata acgttggcaa ctcaaggcat attgatcggt gttcacattg
     1261 cttgctcgat aacgctgttg aaataaatgg ccttttcgat tttgcatctt gttatattca
     1321 aaggaaaagc gccaacagaa atcatgcatg aatttgggaa gatcagtata aacatcgata
     1381 tgaaaaatga aatgagcgtg attactcatc cattcgtagt cagtgagttt gatagtggga
     1441 tatttcttca aatacatccg acagattcta ttgaatagct caaaatgtct ttcttcaaag
     1501 aatcggaact caccgttgtt gatttgtatt ctgacatgca agagcatgtc attggccaaa
     1561 tagtcaaaac gattgattct gggcataatg caaaaatata agcaaagaag atgccaatat
     1621 taaaaaattt attatgttta aattacagcc agatagaaaa aacagaccgc gaaagaaaat
     1681 gtgccattct cagtattgag acacaaaaat gggacgctaa caagcatgtg tgagacaaag
     1741 agacaaagta tgtggcaccg ttctgtgaga caaagtatgt ggcaccgttc tgtggcaccg
     1801 ttcaggtatg tggcacctag aaaattgcta atgtaaaatg tatgtggcac ttaaattagg
     1861 tatgtggcac ctagaaaatt gtccattgaa cctctggggt gggtagcgta tagcctcttt
     1921 catgatagaa cgctactccc gaccagagat ggtccaaatc tgggagccgg aaaacaaatt
     1981 caaaatctgg ctcgaaattg aattgttggc gctggaggcc tgggccaaag aaggggctgt
     2041 gccacaaaaa gccgttgcga tttgtcgcaa aaaggccaat ttcgacgtgg cccgtatcga
     2101 tgagctggaa aaaaccatca aacacgatat gatcgctttt ctcaccaatg tgaatgaaaa
     2161 cattggtcct gaggcgaggt atttgcacaa gggcatgacc tcatctgatg tgctcgatac
     2221 ttcgtttgct tatcagctta agcaggccgg ggacatccta aaaaaggatc tcgatcagtt
     2281 gctcaaggtg ctcaaacggc gcgctcacga acataaaaat acggtgcgca tgggacgctc
     2341 gcacggcgtg cacgccgaac ccaccacctt tggactcaca ctggcgatgt attatgatga
     2401 gttcaagcgt gcccgcaaac gcttggatga tgctactgaa caggtgtctg ttggtaaaat
     2461 atccggcgcg gtgggaacgt tcgcacacat cagtccccga gttgaggaat atgtctgccg
     2521 taaattggga ctcaagccgg caccggtatc aacacagatt gtgcaacggg attctcacgc
     2581 ccattttttc accgtgctgg ccgtgatcgc cgcatcgatc gaaaaactgg ccacggagat
     2641 tcgccacctc caacgcaccg aggtgtatga ggccgaggaa tactttagtc ccgggcaaaa
     2701 agggtcatcg gcgatgcctc acaaacgcaa tcctgtgctg actgaaaatc tgtgcggtct
     2761 ggcgcgtgtt ctgcgcggtt atgctgtgac agccatggaa aacgtggcct tgtggcacga
     2821 gcgcgatatt tcgcacagct cggccgagcg catcattgcg ccggatgcga ccgtgaccct
     2881 tgattttatg ctctctcgcc tgacacaagt catggacaaa ctgttggttt atcccgataa
     2941 catgatggcg aatctcaatt ggc
//
LOCUS       DATDUN010000588         1905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_280114, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000588 DATDUN010000000
VERSION     DATDUN010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280114"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VJC18_08270"
     CDS             complement(<1..635)
                     /locus_tag="VJC18_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HLD45415.1"
                     /translation="MHKEYHKWFSPHLGQDMELNVYGHGGIPFVVFPSSSGRFYNYED
                     FGMVHALEEFINEGKLKLFTVDGIDGQTWYNYAALPGDRSARHDAYDRHILAEVVPFI
                     KTHCQDNDIGIYTTGCSLGAYHAVNFFLKHPDVFNGTIAQSGLYRLDRFQFKIEAHDI
                     PYVYFNSPIHYLANLEDEKILANMRKSKVIVSVGQGAWEHEMVEDTRLLQE"
     gene            complement(755..>1905)
                     /locus_tag="VJC18_08275"
     CDS             complement(755..>1905)
                     /locus_tag="VJC18_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HLD45416.1"
                     /translation="LSPGATPHYHKFCVELKKLGATVLGLGDEVYDQLHPDLKSSLTE
                     FYRVNDLHHYGDLVRAMGYLTFKHGKIDRLDSLAEYWLETEAKLRTDFHIEGIKTDSI
                     ESIKRKSHMKRVFQKAGLEVARGEVLRSVEDVQKFVKLVGYPVIAKPDVGVGALGTFK
                     IRDSKELETMFNTKPANVDYIFEEFIESRICTFDGLVDVAGQIVYCNSMIYSQGIMET
                     VNDDLDVYYYTARDIPLALQTVGERIVRAFDLKARFFHFEFFKLSDDHYVPLEVNMRP
                     PGGYTVDMFNYAADVDLYKAWAEIMLTGKTKLEYDRKYHAVYISRKSRRAYEHSKDQI
                     MQKYGAHILNHVSVPGVFSSALGNEGYLYRADSTQKVLEAAEYIRQKY"
BASE COUNT          536 a          496 c          388 g          485 t
ORIGIN      
        1 atttcctgca agagcctggt atcctcaacc atttcgtgtt cccaagcccc ttgcccaacc
       61 gatacaatca ccttgctttt ccgcatgtta gcaaggattt tttcatcttc cagattagcc
      121 agataatgaa tgggggaatt aaaataaacg tagggaatat catgggcctc aattttgaat
      181 tgaaacctgt ccagacggta caaaccactc tgggcgatcg ttccgttgaa cacgtccgga
      241 tgtttgagaa aaaaattaac ggcatgatag gccccgagcg aacaacccgt tgtatagatc
      301 ccgatgtcgt tatcctgaca atgtgtttta ataaacggga ctacctcagc cagtatgtgg
      361 cgatcatagg catcgtgccg ggcactcctg tctccgggca gggcggcata attgtaccag
      421 gtctgcccat cgatcccatc gaccgtaaac agcttgagtt tcccctcgtt gatgaattct
      481 tcgagggcat gcaccatccc gaaatcttcg tagttgtaaa accggcctga agaactggga
      541 aaaaccacga agggaatccc gccatgcccg taaacattga gttccatgtc ctgtcccaga
      601 tggggactaa accatttgtg atattctttg tgcataaata tttacgccaa catggcaaat
      661 ccgggcacaa ggcgaaaggc gcagggcata aggcttaaaa aaacaattct tcaccttgag
      721 ccttaagcct tacgccttga gccctgaatc agattcaata tttttgtctg atatactccg
      781 ccgcttcgag aactttttgg gtggaatcgg cgcggtacaa atacccttcg ttacccaaag
      841 ccgaactgaa cacaccggga acactcacgt gattcaaaat atgcgcacca tatttttgca
      901 tgatctgatc ttttgaatgt tcatacgcgc gccgtgattt acggctgatg tacaccgcgt
      961 gatacttgcg atcatattca agcttggttt tgccagtcaa catgatctcg gcccaggcct
     1021 tatacagatc aacgtcagcc gcataattga acatatccac cgtataacct cccggcggtc
     1081 tcatattgac ctcaagggga acgtagtgat catcagacaa tttgaaaaat tcaaaatgaa
     1141 aaaaacgtgc cttcaggtca aacgctctca caatgcgctc accaacagtc tgtaacgcca
     1201 agggaatgtc tcgagctgta taataataca catcaagatc atcattgacc gtttccatga
     1261 tgccttgaga gtagatcatt gagttgcaat acacgatctg gcccgcaaca tccaccagcc
     1321 cgtcaaaggt gcaaatcctg gactcgataa actcctcaaa aatataatcc acgtttgccg
     1381 gttttgtatt aaacatggtc tcaagttctt tggaatcacg aatcttgaat gtacccaagg
     1441 cccccacccc cacatcgggt ttcgcaatca caggataacc aaccagtttg acaaattttt
     1501 gaacatcctc aacagatcga agaacttcac cacgagccac ttctaaaccc gctttctgaa
     1561 acacacgttt catatgcgat ttgcgcttta tgctttcgat cgaatcggtc ttgataccct
     1621 caatgtgaaa atcggtcctg agtttcgcct ctgtctccaa ccaatactct gccagcgaat
     1681 ccagacgatc aatcttgcca tgcttgaaag tcaaataccc catggcacgc accagatcgc
     1741 cataatgatg cagatcattc acgcggtaaa actcagtcag tgatgatttg agatcgggat
     1801 gcaattgatc atacacttca tcgccaagac ccaatactgt ggcccccagt tttttgagct
     1861 ccacacaaaa cttgtgataa tgtggcgtgg cgccggggga caaaa
//
LOCUS       DATDUN010000589         2736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_564328, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000589 DATDUN010000000
VERSION     DATDUN010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564328"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..769)
                     /locus_tag="VJC18_08280"
     CDS             complement(47..769)
                     /locus_tag="VJC18_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HLD45417.1"
                     /translation="MQTVILAAGKGVRLEAVNADLPKSLVPIAEGKPYLWYTLTEVDR
                     LTRGRKIIVTGHLSNKMGAFLNRFGFVDYDVVFNACYEWGNLYSLLCVRSEIQDTFLL
                     FNADHHYATETYEKMFSHVSDEITVFCDHDRILTDDDMKVDAHEGLLVTMDKKLICYQ
                     LGYVGVTYVPHGRLSQYWAACDATGTRLGEKANVEAVLNELVTRGEKIAICDISGSWW
                     TEIDTPEDWEKARRVIFVNRGS"
     gene            complement(751..1392)
                     /locus_tag="VJC18_08285"
     CDS             complement(751..1392)
                     /locus_tag="VJC18_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLD45418.1"
                     /translation="MEEKKKIIIFDLDGTLVDSMGDFAVVASTVLHKHFGVSLEEGAR
                     LYRQTSGLPFVQQLEKLFPGAPSNLQAERDFEELKLAGYFEKPFYPEVRSVISSLRER
                     GLRICVSSNNLAENVTKKMEPLRDLFDLILGFGNGLAKGKPHFEHIKKHFGISADELL
                     FVGDSLHDARCAAENNIRFVAKLGTFTQSEFEVLGLPLTFIACLEELLCKLSS"
     gene            1698..>2736
                     /locus_tag="VJC18_08290"
     CDS             1698..>2736
                     /locus_tag="VJC18_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HLD45419.1"
                     /translation="MKIALVPLNPTVGDIPANVDKILGFIDSAIAQNCDLIIFPEMSL
                     IGYPAKDYLFYPWVLYQQQASIAKLKRRSKNIGIVVGGLGRHKGIGRPFLNQAFIFHN
                     GQKHTYTKQLLPNYDVFDDWRYFSPGTEPLILKINGKRFGFTICEDIWFSEDQIKTQY
                     PQDPLNQYRDRKLDFLINISASPFEFDKPPRRAKLISHISQTLHCQVIYVNQSGANDD
                     LIFDGSALVCHTNGKFIYKAPPFTEDLFVFNTDEPRIFLPLKSSKSTKYELLEQALIT
                     GIRDYFGKMGFTQAALGLSGGVDSALVATLAAKALGPENVLGVMLPSRYSSDHSITDS
                     QSLAKQLGIQTM"
BASE COUNT          742 a          785 c          549 g          660 t
ORIGIN      
        1 cgattcacga tccacgatcc acgatttacg atccacgatc cacgatctac gatccacgat
       61 tcacgaaaat cacccttctc gccttctccc aatcttctgg cgtatcgatc tccgtccacc
      121 acgatccaga aatatcacag atggcgattt tttctccccg cgtaaccaac tcgttcaaca
      181 cggcctccac attagccttc tctcccaaac gtgtgcccgt tgcgtcacat gccgcccaat
      241 attgtgaaag cctaccgtgt gggacatagg tcacaccaac ataccccagc tgataacata
      301 tcagtttctt gtccatcgtg acaagcaaac cctcatgagc atcaaccttc atgtcgtcgt
      361 cggtcaaaat acggtcatga tcacaaaaaa cagtgatctc atcggaaaca tgcgaaaaca
      421 ttttctcgta cgtctctgtg gcatagtgat gatctgcatt gaacaacaga aaagtgtcct
      481 gtatttctga gcgcacacac aacagtgagt acagattgcc ccactcatag cacgcgttga
      541 acacaacatc ataatccaca aaaccaaagc ggttcaaaaa cgcccccatc ttgttcgaca
      601 aatgccccgt cacaatgatt ttgcggcctc gtgtcaggcg atccacctcg gtgagcgtgt
      661 accacaaata gggcttgcct tcagcaatgg gaaccagact cttggggaga tcggcgttca
      721 cagcttccaa acgcactccc ttgcccgcgg ctaagatgac agtttgcaca gtaattcctc
      781 caaacacgca ataaatgtca gcggcaaccc caagacctca aactcacttt gcgtaaaggt
      841 ccccagtttg gccacaaaac gaatgttgtt ctcagcagca caccgcgcgt catgcaggga
      901 gtcccccaca aaaagcagtt cgtcggcaga aatcccaaaa tgttttttga tgtgttcaaa
      961 atgaggcttt cctttggcca acccattccc gaatcccaaa atcagatcaa acaaatcgcg
     1021 caaaggttcc attttttttg tgacattctc tgccaaattg tttgacgata cacaaatgcg
     1081 caatccccgc tcacgcagtg atgatatgac tgatctcacc tcgggataaa aaggcttttc
     1141 aaaataccca gccagtttca attcttcaaa atcccgttcg gcctgcagat tgcttggtgc
     1201 accgggaaac agcttttcca gttgttgtac aaacggcagg cccgatgttt gcctgtacag
     1261 gcgggcacct tcctcgagcg acacaccaaa gtgtttgtgc aacacggtac tcgccacaac
     1321 cgcaaaatcc cccatcgagt cgaccaaagt gccatcgaga tcaaaaatga tgattttctt
     1381 tttttcctcc acccctaccc tctctcacca cgcgtaaaaa aaggcactca tcataacatt
     1441 tcggacactg ctcaagacat gttttgaaga cacttcacaa aattcatgac ccgtgacaaa
     1501 ataaccatca tgaattttgt gggcgaaaaa atttgcaagc ggcaaatttt tgagtggtgt
     1561 ctgaaaaaca tgtcttgagc agtgtccgtt tgcctacatg ttagccttgc cttttttcga
     1621 gtccccgcct taaactcaaa cttggaaccg aaatcaagct tgaagcttga taaaactcag
     1681 tctatggctg gcgcataatg aaaatcgccc tggtcccact caaccccact gttggagaca
     1741 tccccgcaaa tgtcgacaag attctgggct tcattgacag cgccattgcc cagaactgtg
     1801 acctcatcat tttccccgaa atgtcgctca ttggataccc cgccaaggat tacctgtttt
     1861 acccctgggt tctgtaccaa cagcaggcct caatcgccaa actcaagaga cgttcaaaga
     1921 acatcggcat cgttgtggga ggcttgggaa gacacaaggg aatcgggcgc ccttttttga
     1981 atcaggcgtt tatttttcac aacgggcaaa aacacactta cacgaaacag ctcctgccca
     2041 attacgatgt tttcgatgac tggcgttatt tctctcccgg aacggagcca ctcatcctca
     2101 aaatcaatgg caaaaggttt ggattcacca tttgcgagga tatctggttt agcgaggacc
     2161 agatcaaaac acaatacccg caggaccccc tcaaccaata ccgtgaccgg aaactggact
     2221 ttctgatcaa catctcggcc tcaccgttcg aattcgataa acccccgcga cgtgccaaac
     2281 tcatcagcca catcagtcag accctgcatt gtcaggtgat ctacgtcaac cagtcgggag
     2341 caaacgacga tctgattttt gacggcagtg ccctggtttg tcacacaaac ggcaagttca
     2401 tctacaaagc cccgcccttt acggaagatc tctttgtttt taacacagat gaaccccgca
     2461 tttttctacc cctcaagtca tccaagagca ctaaatacga actgctcgaa caggcgctca
     2521 tcacaggcat cagggattat tttggcaaaa tgggatttac tcaggccgcc ttgggcttgt
     2581 ctggcggtgt cgattcggca ctggtggcca ctttggcggc aaaggctttg gggcccgaaa
     2641 atgttctggg agtcatgttg ccctcgcgct attcatcgga tcacagcatc acggattctc
     2701 agtccctggc caagcaactg ggcatccaaa ccatgc
//
LOCUS       DATDUN010000590         1164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_232904, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000590 DATDUN010000000
VERSION     DATDUN010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232904"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VJC18_08295"
     CDS             complement(<1..375)
                     /locus_tag="VJC18_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HLD45420.1"
                     /translation="MKLMNIVLVILLVTSLACSAKQKARSDEDSQPTPKEVKQEVAPP
                     QEEAGDKVVEQKVEESITPPDAPVEETKPEVGAPSVVEAPVIEPPVVEKPIVEAPVVE
                     TPVVEKPVADIPMIEKPVAAEPD"
     gene            complement(441..974)
                     /locus_tag="VJC18_08300"
     CDS             complement(441..974)
                     /locus_tag="VJC18_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HLD45421.1"
                     /translation="MKHELKGTQTEKNLQAAFAGESQARNKYDYFAKVARNEGFHYIA
                     NVFEETALNEKQHAKDEFKKLGGLGDTKANLKEAWEGENYEVTKMYPEFAAKAEEEGF
                     AEIASLFKQISKVEAEHRARYQHLLELVEQDKVFLRNAPVKWKCLKCGYVHEGNTPPA
                     KCPTCQHPKEYYAPNDI"
BASE COUNT          252 a          313 c          247 g          352 t
ORIGIN      
        1 atcaggttcg gccgcaacag gtttttcgat catcggaata tcggcaacgg gtttctcgac
       61 cacgggagtt tccaccacag gggcctcaac aatcggtttt tcaacgaccg ggggttcgat
      121 caccggggcc tcgaccacag acggggcgcc cacttcgggc ttagtctctt caacaggggc
      181 gtcggggggg gtaatggatt cctcaacctt ttgttctaca accttgtcac cagcctcttc
      241 ttgaggaggg gcaacttcct gcttgacctc tttgggagtt ggttgagagt cttcgtccga
      301 tctcgccttc tgcttggcag aacaggctaa gctcgttacc aataatatca ccagaacaat
      361 gttcatcagt ttcataaatt cctccagttt tatctgccac cttacccctt ataccttatg
      421 ccttatgcct tttacatctt tcatatatca ttcggggcgt aatactcttt ggggtgctga
      481 cacgtcgggc actttgcggg cggtgtattg ccctcgtgca cgtacccaca ttttaaacat
      541 ttccacttaa cgggggcatt gcgcaaaaag accttatctt gttcaaccag ttccaacaaa
      601 tgttgatacc tggcacgatg ttccgcctca accttggaaa tctgcttgaa caaggaggca
      661 atctcggcaa agccttcttc ttcggccttg gccgcgaatt cggggtacat ttttgtgacc
      721 tcgtagtttt caccttccca ggcctctttt aaattggctt tggtgtcgcc cagaccacca
      781 agttttttga actcatcttt ggcatgttgt ttttcgttga gggctgtttc ttcaaatacg
      841 ttggcaatgt agtgaaaacc ctcattccga gccaccttgg caaaataatc gtatttgttt
      901 ctcgcctgcg attctccggc aaaggccgcc tgcaaattct tttctgtctg tgttcctttt
      961 aattcatgct tcatattttc tccattatat gtttacgtca tatatcttgt cgtgaacacg
     1021 taaacacgtg aacacttaaa cacggtcact aatactccta cactccaaat aatacctctt
     1081 ctcactggcc tcacccattg agaaggtctt gcggggcagg gcgccgtcaa caatcacagt
     1141 cctgaacaga tcatgcttgc tcat
//
LOCUS       DATDUN010000591         1143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_70430, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000591 DATDUN010000000
VERSION     DATDUN010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70430"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            929..>1143
                     /locus_tag="VJC18_08305"
     CDS             929..>1143
                     /locus_tag="VJC18_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45422.1"
                     /translation="MERFIGIRHRVKKTTAGQARPTQIFILGGGKKGQDLVLDLPDEQ
                     SELDFAHGVLVAAMRDVKMGEDVSIFPP"
BASE COUNT          279 a          230 c          338 g          296 t
ORIGIN      
        1 aaggccgggc ttgcttgcac gctaagctat tgacaaaaaa tgccaatctg atagggtaaa
       61 aagtcaatct aaagaaaggt tgatagggcg cgacgtaagc aggtctgtcc ttttgggatg
      121 acgttcgggc tgtcgcgtcg gttatttaca aatgtgagga cgaatttcag tctgtcttgt
      181 tggacgaggg gctgggtgtc ctcacagaca attttgcagg gacgagaagt gcattggctt
      241 ttatggccga gtgatgcaat gtccccgttt gatttttggg agtggacgag aggtaggatg
      301 tttttaaacg cggctcattg tgtccgcttt cattatacaa ggcggtgacg aagaggattg
      361 tgtttttgta aaaacgtgtg ttcaactgtc atcgccagag agtttagcgg ccgcatcaac
      421 tgctgtccca aaggacgaat agctaagtgg ccgcttaata atttgatgga cgatagttgg
      481 gatgtccctg ggacgctcct gcttctgtcc atcaatagtc aaaacgcgga cgagtcaatg
      541 gatggccttt aggccagttg aatagttgtc cgcgtgtaca ttgcgctgac ggtagtagga
      601 agtgtctttg ttagaagacg aaagtcgtac tgtcagcgca taaaatggtg gcgacgaagc
      661 tgaaaatgcg ctttggtgcg tttggaaatt tgttgccatc agaaattttt gggcaggcga
      721 acctaccatt gttggcaaaa gcaacgatcc ggaaggtgtt tgcccatttg ttcaaagacg
      781 gacgaatgtt gcgctgtctt aaacaggcgt accgaggagg tgtccgtctg gttcctaatt
      841 gtccttaagc caaggcggct ttaaggacgg gttccttaca agtgtccctt aggggacata
      901 ctgccccaaa agggcaagga gaagtgagat ggaaagattc atcggcatca gacaccgggt
      961 caagaaaacc acggccgggc aagcgcgacc cacccagatc ttcatcttgg gtggcgggaa
     1021 aaagggccag gatttggttt tggacttgcc cgacgagcag tccgagttgg attttgccca
     1081 tggcgttttg gtcgcggcca tgcgtgacgt gaaaatggga gaagatgttt ccatttttcc
     1141 gcc
//
LOCUS       DATDUN010000592         2296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_280757, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000592 DATDUN010000000
VERSION     DATDUN010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280757"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1350)
                     /locus_tag="VJC18_08310"
     CDS             complement(295..1350)
                     /locus_tag="VJC18_08310"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HLD45423.1"
                     /translation="MDELKKILNINIASHEKLITPSHMKSAVPVYKDALSNVLISRQI
                     IQNILDGRDDRLFFVVGPCSIHDPKAALEYANLLKQLAKRVADKFFMIMRVYFEKPRT
                     TIGWKGLINDPHMDDSFHIEEGLKQARTLLNQINQLGLAAGTEALDPITPQYLAELIT
                     WAAIGARTAESQTHRELASGLSMPIGFKNGTDGNISVAINAMKSATNSHHFLGINEEG
                     QVSVFKTRGNPYSHVILRGGASNTNFDRQSILDCVGELERNNLRQKIVIDCSHGNSGK
                     DIEKQSRVLTDGIQQILDGNHAITGFMIESHLHAGNQKLDHPSKLKYGVSITDACLDW
                     KTTEKMLLEAYKKLKRP"
     gene            complement(1520..>2296)
                     /locus_tag="VJC18_08315"
     CDS             complement(1520..>2296)
                     /locus_tag="VJC18_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HLD45424.1"
                     /translation="FRSFDGKIPFLNDHLGRLQWSSTFLGIEYPAEIDFSTVCQELIA
                     KNNYQNARLKIVLSQTFADYSQATCAPTEIDTKVMTANVTIFCERLLDEALSGPDRLK
                     VIRDFPNEHLPLVAIKTTNYLSKMRARLEAQESGFYDGILLNARGLVTETCTGNIFWI
                     DKDSTLHTVMEEQGLLGGITKKNLIKLLTKHKMDVKQTVITPQELSHAKEVFITNSVV
                     GVKPVVAIDDRQISGGEAGDITLMIMELWKKYLNELVDLS"
BASE COUNT          547 a          515 c          581 g          653 t
ORIGIN      
        1 taatatactg atttgtcgat tgaaattcaa actgacagga ctaatcatat gggctccata
       61 aatagctgac tgtttacggt ctttggctaa cggttggcgc ttgatgactg gctgttggcc
      121 gtaagctgtt ggccgttggc attatacctt gtgcttttca tacattgtgt cggaagatgt
      181 acgggtgagt caatagaaaa agctaataat gtcaatgaat tatagttttt gtctaaatca
      241 cgataagggt tgggagaaaa attaaaatcg catcaggtca agtgcttgat acgtctatgg
      301 gcgtttcagt tttttgtagg cctccagaag catcttttct gtcgtcttcc aatcgagaca
      361 ggcgtcggta atagaaacac cgtatttcaa tttggagggg tgatcgagtt tttgattacc
      421 agcatgcagg tggctttcga tcataaaccc cgtgatggcg tggttgccat cgagaatctg
      481 ctgaatgccg tcggtgagga cgcgggattg tttttcgata tcctttcccg aattgccatg
      541 tgagcaatca atgacaatct tctggcgaag gttgtttctt tcgagctcgc cgacgcagtc
      601 caaaatgctt tgcctgtcga agttcgtatt gctggcaccg ccccgcaaaa tcacgtgaga
      661 gtaaggattc ccgcgtgtct tgaagacgct gacctgtccc tcctcattga tgcccagaaa
      721 atgatgcgag ttggtggcag acttcatggc attgatggcc accgaaatat tgccatcggt
      781 tccattttta aaaccgatcg gcatggacaa accgctggcc agttcgcgat gggtctggga
      841 ttccgccgtg cgagccccga tggcggccca ggtgatgagt tcggccagat actgcggtgt
      901 gatgggatcg agtgcctcag taccagcggc caggcccaac tgattgattt ggtttaagag
      961 ggttcgtgct tgtttgagac cttcctcgat gtgaaaggaa tcatccatgt gagggtcgtt
     1021 gatgagcccc ttccagccaa tcgtggtgcg gggtttttca aaatacacgc gcatgatcat
     1081 gaaaaatttg tcggccaccc tcttggccag ttgcttcagc aggttggcgt attcgagagc
     1141 ggccttggga tcgtgaatgg aacaaggccc cacaacaaaa aacaatcggt catcgcgacc
     1201 atcaagaatg ttctggatga tttgtcggct gatcagcacg ttggataagg catctttata
     1261 gacaggcaca gccgacttca tgtggctggg ggtaatgagt ttttcgtgac tggcgatgtt
     1321 gatgtttaat atttttttaa gttcatccat aatttacgta aattgttttt ctgggtcctg
     1381 ccccggctgg ctattttttg acgcttttcg cttcagccaa aggactgatc ccgctctgcg
     1441 ggacacaagt tcgctccaca ggccgcgtca atataatagc tcgccgtgtc acccagaaaa
     1501 acaatgctcc cacaacactt catgacaaat ctacaagctc attcaaatac tttttccaca
     1561 gctccatgat catgagggtg atgtctcccg cttcaccgcc agaaatctgt ctgtcgtcga
     1621 tggccactac aggtttgacg cccacgacgg agttggtgat gaagacctcc ttggcgtgtg
     1681 atagctcctg tggtgtgatc acggtctgtt tgacatccat tttatgtttt gtcagcaatt
     1741 tgatcagatt ttttttggtg atgccgccca gaagcccttg ttcctccatc accgtgtgca
     1801 aagtgctgtc tttgtcgatc caaaaaatat tgccggtaca ggtttcggtg accagccctc
     1861 tggcgttcaa caaaatgcca tcgtaaaaac cggattcttg cgcttcgaga cgggccctca
     1921 ttttgctcag ataatttgtg gttttgatgg cgacgagagg caggtgttcg ttcggaaagt
     1981 cgcgaatgac cttgagacgg tcaggtcccg acagggcttc atccaaaaga cgttcacaaa
     2041 aaatggtgac atttgcggtc atgactttgg tatcgatctc ggtgggggcg catgtcgctt
     2101 gggaataatc agcaaaagtc tgggacaaaa ctattttcaa gcgtgcattc tgatagttgt
     2161 tttttgcgat cagctcctgg cacactgtgg aaaaatcaat ttcggctggg tattcgattc
     2221 ccaaaaaagt tgaagaccat tgcagacggc ctaaatgatc gttcaaaaat ggaattttac
     2281 catcgaaact tctgaa
//
LOCUS       DATDUN010000593         4180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_442746, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000593 DATDUN010000000
VERSION     DATDUN010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442746"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VJC18_08320"
     CDS             <1..474
                     /locus_tag="VJC18_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HLD45425.1"
                     /translation="MLEGLIQRLEEKKLDLPNASQLTQSCDFWDLVHPRIVGISRSRL
                     ESGHYADSVEAAFKEINDCVKKIVKKRIDDDLDGSTLMNRTFSVNNPVISLDDLSTEN
                     GRNIQVGFMQIFSGSMTGIRNPKAHSNITIDDKRAKHFIVLASLLMHKLDDALGI"
     gene            505..873
                     /gene="rhuM"
                     /locus_tag="VJC18_08325"
     CDS             505..873
                     /gene="rhuM"
                     /locus_tag="VJC18_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HLD45426.1"
                     /translation="MNQKTSKSLTTTSGKGQFLVYSTENGEIKIDVRLENETVWLTQQ
                     LMAELFQTTKQNISLHIQNVLEESELTPETTVKEILTVRQEGDRNVQRNLEYYNLDMI
                     ISVGYRIKTPFFLIQNNALV"
     gene            965..2317
                     /locus_tag="VJC18_08330"
     CDS             965..2317
                     /locus_tag="VJC18_08330"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD45427.1"
                     /translation="MKRKLLYNLMDWKSSPDRKPLLLRGARQVGKTYLLKEFGRMSFS
                     NFHYVNFETENKFSEIFAASLNPKQIIKDLAIKLEKDINIKTDLLIFDEIQACPRAIT
                     SLKYFCEELPDLAVCCAGSLIGVLLSPEPFPVGKVTFLNLFPMTFEEFLWASDAKTSP
                     ALFDEFVQTKTISATAEEHLNAKLREYSFIGGMPQAVCAHCQEPGDRAVNKTMLIREI
                     QKTLLKSYYADFAKHSGKINAMHIVSVFENVPLQLARELDGSVTRYKFQDVVPGKKGF
                     ADLAGPIDWLVNAGLIHKVKICNHPEIPLSAFCKPNAFKLYYFDIGLLGCALNLPMES
                     FFGQEFGMAKGFLAENLVCQELIANTDAPLHAWTEQNSEIEFLLQIKDQVVPVEVKAG
                     NRTRAKSLQVYLQKYKPKLATMLSHRPPRIDSHRVLQHIPLCLAGVLENICDQLLFRP
                     "
     gene            2445..2714
                     /locus_tag="VJC18_08335"
     CDS             2445..2714
                     /locus_tag="VJC18_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45428.1"
                     /translation="MPCFQPISGLIHFGQATLARVLPGYATQQAGVSHAVTDRYALVG
                     SESTATTAPPPCHGCQRNSSARKNKKNTTPAKTNRGILLCNKLGK"
     gene            2749..3495
                     /locus_tag="VJC18_08340"
     CDS             2749..3495
                     /locus_tag="VJC18_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45429.1"
                     /translation="MALDPAAPAQTIQGEVGQRAVSGLPALRSFDAGVFARFHNIQPE
                     NLRDTSRFVFGVPSGAGCDSFQITDTRRYNEGNVYLLRHLSNLFDLQVVAQTHPDHYS
                     VARGVHCQMAPNKVEYTVIDDALTIRISRAGTEVLTGEERAWVQSRIELAERIALLVT
                     PEGVGKKNALAEPEQRPNTRVSRSILKDMDKTLSDQRKLGIHAYGPTTLRGGQHEQAV
                     NEGINIFIAALINVFEMSDADVAWLTATLK"
     gene            complement(3554..>4180)
                     /locus_tag="VJC18_08345"
     CDS             complement(3554..>4180)
                     /locus_tag="VJC18_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HLD45430.1"
                     /translation="QNRIVSVCRDLKKPVIVATQMLSSMRWEAIPTRAEVSDVATAVY
                     LGADGVMLTGETSVGKHAVAAVKMLGQIISQTEQVPLSVPTPAPAYDKDTLAETILAN
                     VCQLAKDVRAKAIVVHTTRGQMTGILSKLGVAIPIFACTENEHLYRRTTLFSGIIPIM
                     VDKRMSCGQGNDIIAHLKALKYLRTNDLVVFVERVAKTNSVNVEITAI"
BASE COUNT         1195 a          976 c          928 g         1081 t
ORIGIN      
        1 atgttagaag ggctaattca gcgtttggaa gaaaagaagt tagatcttcc aaatgcatcc
       61 cagcttactc aatcatgcga cttttgggat cttgtacatc cacgcattgt tgggatttcc
      121 cgcagtcgcc ttgaaagcgg acattatgct gattcggtag aggccgcttt taaggaaatc
      181 aacgattgtg tgaaaaaaat tgtaaaaaag agaattgatg atgaccttga tggcagcact
      241 ctgatgaatc gtactttttc ggttaataat cccgtgatta gtcttgatga tctctccact
      301 gaaaacggaa gaaatatcca agtgggattt atgcaaattt tttctggctc aatgaccgga
      361 ataagaaatc caaaggcaca ttccaatatc accatcgatg ataaacgagc taagcatttt
      421 attgttcttg ccagtctgtt aatgcacaaa ctcgatgatg ctttgggcat atgagccgtg
      481 gaatgaaatg aaagtggaac aaccatgaac caaaagacat caaaaagcct gacaaccacc
      541 agcggcaagg ggcaattcct tgtctattcc acagaaaatg gcgagatcaa aatcgatgtg
      601 cgactggaaa acgaaaccgt ctggctaacc caacaactca tggcggagct tttccagaca
      661 accaagcaaa atatcagcct ccatatccaa aatgtccttg aagaatcgga acttacgcca
      721 gagacaactg tcaaggaaat cttgacagtt cgccaagagg gtgatcgcaa tgtgcagcgc
      781 aatcttgaat actacaatct cgatatgatt atctcggttg gttaccgcat aaaaactcca
      841 ttttttctga ttcaaaacaa tgcgttggtt taaacatatc aaatggttgc gtattatgtg
      901 ccaattattc tgcaaatcca gtacttttac gttgtaaata ttcttgcttg ctgtataata
      961 aggcatgaag cgaaaacttc tatataattt aatggattgg aagtcttctc cagaccgaaa
     1021 gccccttctt ttaaggggtg ccagacaggt tggtaaaacc tatctcctca aagaatttgg
     1081 aagaatgtcg ttttcaaatt ttcattacgt gaatttcgaa acagaaaaca agttttctga
     1141 aatttttgcc gcctcattaa atcccaaaca aattatcaaa gatttggcga tcaagcttga
     1201 aaaagacata aatataaaaa ctgatttact cattttcgat gaaatccagg catgcccccg
     1261 tgccatcaca agtttaaaat atttttgtga agaacttccc gatctggctg tgtgctgtgc
     1321 gggctcactc attggggtat tattatcccc tgaaccattt cccgtgggca aggtcacttt
     1381 tctgaatctt ttcccaatga cctttgagga atttttatgg gcttcggatg caaaaaccag
     1441 ccctgccctc ttcgatgagt ttgtgcagac aaaaacaatc tctgcgacgg cagaggagca
     1501 cctcaacgcg aaattacgag aatacagttt tataggcggc atgccacaag cggtctgtgc
     1561 ccactgtcag gaaccgggcg atcgtgctgt aaacaaaacg atgctgattc gtgaaatcca
     1621 aaaaactctc cttaaaagtt attatgccga ctttgccaaa cattctggca agatcaacgc
     1681 catgcacatt gtttccgttt tcgaaaatgt gccccttcaa ttggcacgag aactcgacgg
     1741 ttctgtgacc cgctacaaat tccaggatgt ggttccgggc aaaaagggat tcgctgatct
     1801 ggcgggacca attgactggt tggtcaacgc cggcctcatt cacaaagtca aaatctgcaa
     1861 tcacccagaa atcccattgt cggcgttttg taaacccaat gcctttaaac tgtattattt
     1921 tgatattggt cttctggggt gcgccctgaa tctgccaatg gaatcattct tcggccagga
     1981 atttggtatg gcaaagggct ttcttgcgga aaatctcgtc tgtcaggaat tgatagcaaa
     2041 caccgatgcc cccctgcatg cttggacgga acaaaattct gaaatagaat ttctcctgca
     2101 gatcaaagat caagtcgttc ctgtcgaagt gaaggcaggc aatcgaacgc gtgcaaaaag
     2161 tttgcaggtc tatctgcaaa aatacaaacc taaattggca accatgttat cacacagacc
     2221 accgcgaata gactcccatc gcgtgttgca acacatcccg ttgtgtctgg cgggtgtgtt
     2281 ggaaaacatc tgcgatcagc tgttgtttcg accttgacca cggctgtcat gtgctattta
     2341 tctgtgtttt cagccacata aaaaaatgag caacatcatg tggtcagtag ccgataattg
     2401 aaatgatatt gatataatga acagaacaaa aaaaggaggt tgctatgccc tgctttcagc
     2461 ccatttctgg tttgatacat tttggccaag cgacactcgc cagagttctc cccggttatg
     2521 caacccagca agcaggggta tcacacgcgg tcacagatcg ttatgctttg gttggatcgg
     2581 agtccaccgc aacaacggcc ccccccccat gccacggttg ccagaggaac agcagtgccc
     2641 gcaaaaacaa aaaaaataca actcctgcaa aaacaaacag gggcattctc ctctgtaaca
     2701 aattgggcaa ataacagaag ggctcgcaaa gccttgccaa tggtatttat ggcattggat
     2761 ccggcggctc ctgctcaaac aatacaaggc gaggttggcc aacgggccgt ttctggtttg
     2821 ccagccctcc gctcatttga tgctggcgtg tttgctcgat tccacaacat ccaacccgaa
     2881 aaccttcgtg atacgagccg gtttgtgttt ggcgtcccaa gtggtgctgg ttgcgattca
     2941 tttcaaataa cagatactcg ccgttataac gaaggcaatg tttatctcct aaggcattta
     3001 tctaacttgt ttgatctgca ggtggtggca cagacacacc cagaccacta ttcagtggcc
     3061 agaggcgttc attgtcagat ggcgccaaac aaggtggaat ataccgtgat tgatgatgcg
     3121 ttaacgataa gaatatcgcg ggcgggcacc gaagtgctta cgggcgaaga gagggcttgg
     3181 gttcaaagtc gaatagaact tgccgaaagg attgctttgc tcgtaacccc agaaggggtt
     3241 ggcaaaaaaa atgccttggc cgaaccagaa caaagaccaa acacaagggt atctcgtagc
     3301 attcttaaag atatggataa aacactttcg gaccagagaa agctcggaat acatgcttac
     3361 ggaccaacaa ccttacgggg aggccaacat gaacaggctg tgaatgaagg cattaatatt
     3421 ttcattgcgg ccctcatcaa tgtctttgaa atgagcgacg cggacgttgc gtggcttacc
     3481 gcaacactca aataatcacc aaacatcaat agcgccacag atctcgtttc gaacaacaaa
     3541 aatttctggc atttcagatc gctgttattt ccacgttgac actgttggtt ttggctaccc
     3601 gttcaacgaa aacaacaagg tcatttgttc tcaggtattt cagcgctttc aagtgagcga
     3661 tgatgtcatt gccctgaccg caggacattc ttttgtcaac catgatcggg ataatcccgc
     3721 taaacagtgt ggtgcggcgg tacaaatgtt cattttctgt gcacgcaaaa atgggaatgg
     3781 caacacctag ctttgataat atccctgtca tttgcccgcg cgtggtgtgg acgacaatcg
     3841 cctttgccct cacgtccttg gccaattgac aaacatttgc caaaatggtt tccgccaaag
     3901 tgtctttgtc ataggcaggg gctggtgtag gaacagacaa gggcacctgt tctgtttgcg
     3961 agatgatctg gccaagcatt ttaaccgctg ccaccgcgtg tttgccaacc gacgtttctc
     4021 ccgtcaacat cacgccatca gcacccagat agacagccgt ggccacatcg ctcacctcgg
     4081 cccgcgtggg gatggcctcc cagcgcatgg aactcaacat ctgcgtggcc acgatgactg
     4141 gtttttttaa atcgcggcaa accgaaacga tccggttttg
//
LOCUS       DATDUN010000594         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_610170, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000594 DATDUN010000000
VERSION     DATDUN010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610170"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VJC18_08350"
     CDS             <1..1282
                     /locus_tag="VJC18_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45431.1"
                     /translation="LAIGRDLSGAEYYVGCDGMGAHIGGDLISAVTAQTVSWRLAHGD
                     SFSQAINFAANVWRDLWGRFQEQIIVDQLVNKCLMSLLQEVEQSDEDVDGYDDDVTEY
                     VLNKGSVEKELSRWICQADLVNSIAEALSQRTFRSGASSEIDDLLTSLLRGREQPIIS
                     PNKMPGTTLVALKKWRTESGTLCMQHVTIGDSRILVYGRKKGSTDPFTLIEQNVPQTC
                     MADLEPFREIARSKGATDHDEIDRMARLYQRRHPMTSKIVGSFNPHFDDVLIDESHPM
                     MLDAAMEYVTLISTDGLTGNVDEYTEIPLVLRAQQRADFMIAGLQQYMTDKRSAERMI
                     LKKLRKAMAPFREHYKMLRQSQSPLEALTAEDHARLASLDDDLRALWDQYGNLLVPES
                     LRFTDPSVPHHSGPFYIAIDGIKYDDSTQIVVMM"
     gene            complement(1287..>2075)
                     /locus_tag="VJC18_08355"
     CDS             complement(1287..>2075)
                     /locus_tag="VJC18_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLD45432.1"
                     /translation="QNLHPKTLIDPKLLAHIPKTTTAQAFDAQKIVKPPRTDPLLKAL
                     LLYHLGFENYAFDESRNSELIKDITDYQTAKTVELSGNFSRGYSMRFLAAKGNLAGAN
                     EAQGFQLSYPVAYPHYVVPFSKIWNLDQNLVLAMMRQESAFKPEAFSFAFANGLMQII
                     PPTGKEIADAIGFEGFHPDLLREPGISVFFGTYYIRHLLDTFDNNMIFAIAAYNAGPD
                     ALKRWTQTSGSLELDEFIELIPYIETGNYVKKVLVNYLVYQRLY"
BASE COUNT          504 a          469 c          594 g          508 t
ORIGIN      
        1 tctggccatc gggcgtgatc tttctggggc tgagtattat gtgggctgtg atggcatggg
       61 cgcacacatc gggggggatc taatttcggc ggtcacggcg cagacagtga gttggcgcct
      121 ggctcatggt gattcttttt cgcaggctat caacttcgcg gccaacgtct ggcgtgacct
      181 gtggggccgg tttcaggagc agatcatcgt cgatcaactt gtcaacaagt gtctgatgag
      241 tcttttgcag gaggttgaac agagcgatga ggatgttgat ggttatgatg atgatgttac
      301 cgaatatgtg ctcaacaagg ggagtgtgga aaaagagctt tcccgatgga tatgtcaagc
      361 tgatcttgtc aacagcattg cggaggcgct ctctcaacgg accttcagat cgggggcatc
      421 gtccgagatt gatgatttgc tcacatcact cctgcgggga agggaacagc ccatcatctc
      481 ccccaacaag atgccgggca cgacacttgt ggcattgaag aaatggcgaa cagagagtgg
      541 cacactctgt atgcaacatg tcacgattgg cgatagcagg attttagtct atggcagaaa
      601 aaagggtagc acagatccat tcacactcat tgagcagaat gtgccgcaaa cgtgtatggc
      661 tgatctggag ccgttcagag agatcgccag gagcaaaggg gccacggatc atgacgaaat
      721 cgatcgaatg gccaggcttt atcagcgcag gcatcccatg acttcaaaaa tcgtggggag
      781 ttttaatcca cacttcgatg atgtgctcat agacgaatcc catccgatga tgctggatgc
      841 ggcgatggaa tatgtcactc tcatcagtac cgacggcctg acgggaaatg tcgatgaata
      901 cacggaaatt ccgctggtcc tgcgggcgca acagcgggcc gatttcatga tcgcggggtt
      961 gcagcaatac atgacagaca agagaagcgc cgagaggatg attctcaaaa aattaagaaa
     1021 agccatggcg ccgtttcgtg agcactataa aatgttgagg cagtcgcagt cccctctgga
     1081 ggctctcacg gcagaggatc acgccagact ggcttcgctc gatgatgact tgcgtgcgct
     1141 ttgggatcaa tatggaaatc tgctggtgcc ggagtcgctt cggtttacag atccctcggt
     1201 ccctcatcac agcggcccct tttatattgc catcgatggc atcaaatatg atgactccac
     1261 acagattgtg gtgatgatgt gaagtttcaa tacaacctct gatacaccag ataattcacc
     1321 agaacttttt tcacataatt gccggtctcg atgtagggaa tgagttcgat gaattcatcc
     1381 agttccagtg agcccgaggt ttgtgtccac cgtttgagcg cgtcaggtcc agcgttgtag
     1441 gcggcgatgg caaagatcat gttgttgtca aaggtgtcga gcaggtgtct gatgtagtat
     1501 gttccaaaaa aaacactgat ccccggttcc ctgagcagat caggatggaa tccctcaaac
     1561 ccgatcgcgt ccgcgatttc cttccctgtg gggggaatga tctgcatgag accgttggcg
     1621 aacgcgaatg aaaaggcttc gggcttgaac gcggattcct ggcgcatcat tgccagcacg
     1681 aggttttggt caaggttcca gattttggaa aagggcacaa catagtgagg gtaggcaacc
     1741 gggtatgaga gttgaaaccc ctgtgcctca ttggccccag ccagatttcc cttggccgcc
     1801 aagaatctca tggagtaacc gcggctgaaa ttgccggata attcaaccgt cttggctgtt
     1861 tggtaatcag taatgtcttt gatgagttca gaatttcggg attcgtcgaa cgcgtaattc
     1921 tcgaacccca gatgatacaa caacagggct ttcaggagag gatcagttct gggaggcttg
     1981 acgatctttt gagcatcgaa cgcctgagct gtggttgtct tgggaatgtg ggccagcagt
     2041 ttggggtcga tgagtgtttt ggggtgcaaa ttttg
//
LOCUS       DATDUN010000595         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_487068, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000595 DATDUN010000000
VERSION     DATDUN010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487068"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VJC18_08360"
     CDS             <1..440
                     /locus_tag="VJC18_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45433.1"
                     /translation="AVSKSRAEIVEMCLRARQLAQYNSDEPFGAGIEFVLQDSDVGEW
                     SLRVTGVHPASEAFQMGIQRDDLIVAVDGLVIRGLSADGTKEGVVDPNTTMVRGFGGP
                     RNSTLVLTIERDGQCEEVAVTRNIWSARTLGVSNDAPAWDGKP"
     gene            711..1319
                     /locus_tag="VJC18_08365"
     CDS             711..1319
                     /locus_tag="VJC18_08365"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HLD45434.1"
                     /translation="MQNTLFIISFLCSFVFFLSSAVAKTDAQVKQESYAVQGATLCEL
                     KKEIHKKGVAGDDGKIWAGYSRWNIAWNFKATAKNKTDCSMEGVHTQADITITLPEWV
                     PPKGTDPAVIAEWHRYVNILKKHERTHAGYATRTAKKIDAELAGIKHFPDCQAVTELA
                     DEKGQHLMESLRIKDREFDLKTNHGRTQGAVLKAECTQRVKD"
     gene            complement(1332..1634)
                     /locus_tag="VJC18_08370"
     CDS             complement(1332..1634)
                     /locus_tag="VJC18_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45435.1"
                     /translation="MPYFAGQIIPWEKSHVRYVGIDRYGAQNTAEELSREHLSDGTGA
                     FLEMGLRLGKGTTYARPHQFDFSGSMDIGANLMMFGGDTAVGVNVGFGFEPRWRQE"
     gene            complement(1886..2266)
                     /locus_tag="VJC18_08375"
     CDS             complement(1886..2266)
                     /locus_tag="VJC18_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45436.1"
                     /translation="MKHVINFVVLFSLLGVANQCHSQTTPVSNMPNPPELVTCDALNE
                     QFANTLGQHSGHCLQDSECACFDAGGYLSTPCGGVTDRYTAVKMNELIKLSIANKCGP
                     AVACAPWLCQPVCKAGFCVNKEPF"
BASE COUNT          572 a          564 c          638 g          607 t
ORIGIN      
        1 ttgctgtttc gaagtcgcgg gccgaaattg ttgaaatgtg tttgcgcgca cgccagttgg
       61 ctcaatacaa ttctgacgag ccttttggtg ccggtattga atttgtcctt caggattccg
      121 atgttggcga atggtcactc agggtcacgg gcgttcatcc ggcgagcgag gcctttcaga
      181 tgggcattca acgggacgat ttgattgttg ccgtggatgg tctcgtgatt cgtggtctga
      241 gtgcagatgg aaccaaagaa ggcgttgtcg atcccaacac gacaatggta cgtggttttg
      301 gaggtcccag aaattcgact ctcgtattaa cgattgaaag ggacgggcag tgtgaagaag
      361 tcgccgtcac gagaaacatc tggtccgcac gtactctggg agttagcaac gacgctcctg
      421 cgtgggacgg aaagccatag ggtgtagctg aataagtttt ttttgtgatt tgatgatcct
      481 gccctaactc acatttgaga accaaaatgg tttaaataaa tttgaatgag tctattcatt
      541 atagacataa gctgatattt ataaaccctt ttgttgacgg agaaagtgtt ggctcagttt
      601 tagtcatact cgtgctgatt ttgttgtgta tagaggcccc ggtgagtgaa ggcatatttt
      661 attaagatta tttttttgcc tctttccctt ttttgtgttt gttgtccgcc atgcaaaaca
      721 ccctcttcat catatccttt ctgtgttcgt ttgttttttt tctcagttca gccgtggcca
      781 aaacagacgc acaagtcaag caagagtctt acgccgttca gggcgccacc ttgtgtgaac
      841 tcaaaaaaga gatccacaaa aagggcgtgg cgggtgatga tggcaaaatc tgggccggtt
      901 attcccggtg gaatatcgcc tggaacttta aggccactgc aaaaaataaa acggattgtt
      961 ccatggaggg ggttcatacc caggcggaca tcacgatcac cttgcccgaa tgggtgcccc
     1021 caaaggggac agacccggct gtgatcgccg aatggcatcg ttacgtcaac atactcaaaa
     1081 aacatgaacg cacgcacgct ggttatgcca ccaggacggc aaaaaaaatt gacgcggagt
     1141 tggccggtat aaagcacttt cctgattgtc aggctgtgac agagcttgcc gatgagaagg
     1201 ggcaacatct gatggagagc cttagaatta aggaccggga gttcgatctc aaaaccaatc
     1261 acgggcgcac acagggcgcc gtactcaagg ctgagtgcac acaaagggtc aaagattgat
     1321 cccttttttt ttcactcctg acgccatctg ggctcaaacc caaaccccac gttaacacca
     1381 acagccgtgt cgccaccgaa catcatcagg ttggcaccga tgtccatgct ccccgagaaa
     1441 tcaaactgat gaggtctggc ataagtggtg cccttgccca gacgcaaacc catctccaga
     1501 aaagcacccg tcccgtcact caaatgctca cggctgagct cttcagcggt gttctgggcg
     1561 ccgtaacgat caatgcccac ataacgcaca tgactctttt cccacgggat gatctggcct
     1621 gcgaagtacg gcatgcagaa ctaaggggtc aaactaaggg gtcaaagatt gacaagggac
     1681 aggttgaggc tcgttgggct tgcttgtctc ttgtcaatct ttgacccctt gttctttgac
     1741 cccttgttct tgttcttgtg gggaggagtc aggggacgcc acgggaatac tcgttgttgt
     1801 tgtcatcatg ccgcctttct tgcgatgttg ctggcccctg ctgttacgct taatgtattt
     1861 gttgcgtgtt tttcaagttt tattgtcaga acggttcttt gttgacgcaa aatccggctt
     1921 tacagacagg ctggcagagc cacggcgcac aggccacagc gggtccgcat ttgttggcaa
     1981 tagacagctt gatcaattca ttcattttga cggcggtgta acggtcggtg acgccaccac
     2041 agggtgtgct caggtacccg ccggcgtcaa agcaggcaca ttcggaatcc tgcaggcagt
     2101 gcccgctgtg ttggccgagc gtgttggcaa attgttcgtt gagagcgtca caagtgacca
     2161 gctcaggcgg gtttggcatg ttgctaacgg gcgtggtctg gctgtgacac tgattggcga
     2221 cccctaatag gctgaacagg acaacaaagt tgataacatg tttcatgctt gaccgttagc
     2281 cctgttttcc aaatacagca aggccaacgt gaaggagggg gcggagggaa aagactcgta
     2341 acaattcagt cccaccaggg cgaggagcat ggccacaaag g
//
LOCUS       DATDUN010000596         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_564714, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000596 DATDUN010000000
VERSION     DATDUN010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564714"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VJC18_08380"
     CDS             <1..1026
                     /locus_tag="VJC18_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLD45437.1"
                     /translation="SFEFSTLGGWLATRSAGQNSTKYGKIENMVQSLVIHTPKGVIET
                     HQVPASATGPSLKHLLLGSEGIYGIITEAVLKIHPLPQSQKFSCAFFENFEQGLTCVR
                     TIMQADITPSVVRLSDADETSFFLDLMLSKKWQKKLLPHWFNFKGLGNHPCFMLISCE
                     GQAAHTNFHLDKIRSLIRSTQGKLVPRKFAAKWKKDRFELPYLRDNLMDHGFFIDTLE
                     TAAPWSKLLGIYESMKLAFFEQKSHQLLMACHLSHAYKDGASLYFTFMGVQKRGHEIA
                     QWEEIKNLATNVIMSQGGTLSHHHGVGYDHKKWMVQEETPVGIEVLKAIKKELDPEGL
                     LNPGKVV"
     gene            1119..>2301
                     /locus_tag="VJC18_08385"
     CDS             1119..>2301
                     /locus_tag="VJC18_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD45438.1"
                     /translation="MPHLRTRHVLANIKKLASLWPLVGVLGPRQSGKTTLLSKLLGVE
                     NMISLDEALLREEAQRSPDLFLSRLSTPVVIDEVQKAPQLFDALKLKVDKKRVPGLYY
                     LTGSSNFSLRIGIRESLTGRLGAVSLTPMTLAELNQKPFFTIKDLANPTQSQKAPRFD
                     LSLVTKSVVSGGMPVPAFLHASEQRHLYWSSWLDTTLHRDLPRQFARGGYDPDLALNL
                     INRLATVFGEGELPSLKHFAQNARKVRRYFSAMEDIFLLRKISCHPLGTGREVWLFFD
                     SGLLAYLLGNTAGEANFLSLVRHFLWNEWLSHTPGLPFEKLYYKSTQGQPVDAVLNNI
                     PFRIVGTTTGLGKQFSWEARPLMGAMKKLGSKIGYLVGPTEIVTAPQKNNGVGILPWG
                     MW"
BASE COUNT          577 a          607 c          553 g          564 t
ORIGIN      
        1 tcattcgaat tctccacatt gggcggctgg ctcgccacgc gttccgcagg acagaactcc
       61 accaaatatg gaaaaattga gaacatggtg caatcactcg tcatccacac accgaaaggc
      121 gtgatcgaaa cacaccaggt ccctgcctcc gcgacaggtc cgtcattaaa gcatctcctg
      181 ctgggctccg agggcattta cggaatcatc actgaggcgg tactcaaaat ccatcccctg
      241 cctcagtcac aaaaattcag ctgtgccttt ttcgaaaact ttgagcaagg gctcacctgt
      301 gtgcggacaa tcatgcaggc agacatcact cccagcgtcg tgcgtctgtc ggatgccgat
      361 gagacctctt tttttctgga cctgatgctc tcaaaaaaat ggcagaaaaa acttcttccg
      421 cattggttca acttcaaggg tctgggcaac catccctgct tcatgctgat ttcctgcgaa
      481 ggacaagccg cgcacaccaa ttttcatctt gataaaatca ggtccctcat ccgttcaaca
      541 cagggaaaac ttgttcccag aaaatttgcc gcaaaatgga aaaaagaccg gtttgaactt
      601 ccttatcttc gagacaatct gatggaccat ggtttcttca tcgacacgct cgagaccgcc
      661 gccccgtggt ccaagctcct gggcatttac gagtccatga agctggcctt ttttgagcaa
      721 aaatcccatc aactgctcat ggcctgtcat ctgtcgcacg cctataagga cggggcttcg
      781 ctctacttca cgttcatggg ggtgcaaaag cggggacacg aaattgccca gtgggaagaa
      841 attaaaaatc ttgccaccaa tgtgatcatg agtcaaggcg gcaccctgag ccatcatcac
      901 ggcgtcggat acgatcacaa aaagtggatg gtacaggagg aaacccctgt gggcatcgag
      961 gtcttaaagg ccatcaagaa agaattggat cctgagggat tgctcaatcc ggggaaggtg
     1021 gtgtgaaaaa atcaaaagat cataaaatgc caataataaa cagcatccaa tgtcgtttat
     1081 tgttggcatt ttgatcgctg atgctgtata atgggggcat gccccacctc cgcacacgtc
     1141 atgttctcgc aaatatcaaa aaactggctt cgctttggcc cttggtgggt gttttgggtc
     1201 ccagacaatc gggcaagacg accctgctca gcaagctgtt gggtgttgaa aatatgattt
     1261 cgctcgatga ggcactgctc agggaggagg ctcagcgctc acccgacctt tttctctccc
     1321 gattgagcac gcctgtggtg atcgacgaag tgcaaaaagc gccacagctc tttgatgcac
     1381 tcaaactcaa ggtagacaaa aaacgtgttc ccggacttta ttacctcacg gggtccagca
     1441 atttttcgtt gcggattggc attcgcgaaa gcctcacggg tcgcttgggc gctgtgtctc
     1501 ttacgcccat gacgctggcc gagctcaatc aaaaaccctt ttttacgatc aaggatttgg
     1561 caaatccaac tcaatcccag aaagcgccac gctttgattt gtcactcgtg acgaagtccg
     1621 ttgtttccgg cggcatgcca gtccctgcct ttttacacgc cagtgaacag cgccatcttt
     1681 attggtcatc gtggcttgac accacactgc atcgcgatct tcccagacag tttgcgcgcg
     1741 gcggttacga tcccgacctg gcactcaacc tcatcaaccg cctggccacc gtttttggcg
     1801 agggtgaact ccccagcctg aaacattttg cgcaaaacgc acggaaggtc aggcgatatt
     1861 tttcggccat ggaagacatt tttctcttaa gaaaaatttc atgtcatccg ttggggacgg
     1921 gtcgcgaggt ctggttattt tttgattcgg ggttgctggc ttacttgctg ggaaacactg
     1981 ctggagaggc caacttcctc agcctggtca gacattttct ttggaacgag tggctgagtc
     2041 atacaccggg gcttcccttt gaaaaacttt attacaaatc aactcaaggt cagcccgttg
     2101 atgctgtttt gaacaacatt ccttttcgca tcgtcggcac cacaacgggt cttggaaaac
     2161 agttctcatg ggaagcccgt cccctcatgg gagccatgaa aaaactcggc agtaagatcg
     2221 gttatctggt tggtccaacc gaaatagtca ctgcaccaca aaaaaacaat ggtgtcggaa
     2281 ttttgccgtg ggggatgtgg a
//
LOCUS       DATDUN010000597         2621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_196089, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000597 DATDUN010000000
VERSION     DATDUN010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196089"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2621
                     /locus_tag="VJC18_08390"
     CDS             <1..>2621
                     /locus_tag="VJC18_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45439.1"
                     /translation="DEVYIRADWAWSAYKMMTGMVGTHNPGDSPATSELAGVTFGNFN
                     AEIAEMQRKATSQDQFIYYVTILEDGTIKHKLLDQPDARRDDITIPYRNLSDLDHKLS
                     GMTPPEDQEQLRIFFQQPRNHRDVNLNLPGMLDQPFKVAPTSDYFFVYTTATGQQFRI
                     LRVPVANQCTTATTSSNYQPLGYLTAEALGFFSTVFNLGTSTGTNSPLNLDPNIGSVT
                     FSVGGEQKIAHFYKSNIQRLSISDNIITLAFEMQEMGAPFDLDPADPKEKVLEKLLEY
                     FENDSNDSDYLPNGEFSDPDAWADEGNRTVSFAGEELPYKLALYLVAMSVKFSTGSEK
                     IGEGYLDITQQGMKKGEQLQYLFMGFILIQTIISVVASGRITKKLYKEAMATQLLQED
                     AARELAKKMRDPHWCPDFMSPMFDSKEIEDPNPYFTTPEREAQLRQVMEALTGKAFSA
                     AFVTGPAGVGKTEMMREIQRRLLLPEQEALSLGVPRPLLGKTIFTIDGNKWDARNGQV
                     GADSKLTAEFILFMRRRPDGIAFFDEAKMLTTSGLWGGGQGEKVKGNSPFEKMLPYLS
                     DRKLYAVFATTTGEYEEFFKGDPRFAAFLRRSEPVELQMFTMDEVKIALATIYPVLLE
                     KLRVISIDQSEPKVDIVVNDESGMRVVSVPRVISDLVDSTAREAYVIENPNVLMDFSI
                     KRLKILVDKVTRDVVDYSTRPDRGRGVLKHPVNHEIGTLLERRDKLKSERDRVKSEIE
                     IALVKSDSFFREIYDKIHTTPELITRLPQRVQTAIKSIVLGGIGTGAVCTSLFVPEQI
                     GWLSVSGISLGAYMQIRFLNILFGEQFRTWINNTPLKAQFDRLFRNLPFWESELGRIN
                     RELENIDSRLLEIGGDP"
BASE COUNT          759 a          618 c          625 g          619 t
ORIGIN      
        1 gagacgaggt ctacatccgc gccgactggg cctggagcgc gtacaagatg atgacgggga
       61 tggtggggac gcacaatccc ggtgacagcc ccgccacaag cgaattggct ggcgttacct
      121 ttggaaactt taatgctgaa atcgcagaaa tgcaaagaaa ggcgacttcc caagaccagt
      181 tcatctatta tgtaaccatc cttgaggatg gcactatcaa acacaaatta ctcgatcaac
      241 cagatgcacg aagggatgac atcacaattc cttacagaaa tttaagcgat ctggaccaca
      301 aactttcagg catgacaccc ccagaagatc aagaacaact gagaatattt tttcaacaac
      361 caagaaatca cagagatgtt aacttgaacc tgcccggcat gcttgaccaa ccctttaagg
      421 ttgcgcccac cagtgattac ttttttgtgt acacaaccgc tacgggtcag caattcagga
      481 ttttacgggt gcccgtagcc aatcaatgca ctacagcgac taccagcagt aactatcaac
      541 ctttgggcta tcttacggca gaggcattgg gatttttttc gacagttttt aatttgggca
      601 catcaacggg cacaaacagt ccactcaacc tcgaccccaa tataggctca gtcactttta
      661 gtgttggagg ggagcaaaaa atagcccact tctacaaatc aaatatacag cgtttatcca
      721 tttcggataa catcatcacc cttgcctttg agatgcagga aatgggtgct ccttttgatc
      781 ttgaccccgc cgatcccaag gaaaaagttt tggaaaaact gctcgaatac tttgaaaacg
      841 actcaaacga ttctgattat ctgccaaatg gtgaattttc cgaccccgat gcgtgggctg
      901 atgaagggaa cagaactgtt tcctttgctg gtgaggaatt gccatacaaa ctagcccttt
      961 atctggtcgc catgagcgta aagttttcca ctggcagtga aaaaatcggt gaaggttatc
     1021 ttgatatcac acaacaagga atgaaaaagg gcgaacagtt gcagtacctg ttcatggggt
     1081 ttatcctcat acaaaccatt atttcggtag tggccagtgg caggatcacc aagaaattgt
     1141 acaaagaggc catggccaca caactgctcc aagaggatgc cgcgcgcgaa ctcgccaaaa
     1201 aaatgaggga tcctcactgg tgcccagact tcatgtcccc catgttcgat tccaaagaaa
     1261 ttgaagaccc taatccctac tttaccactc ctgaaaggga ggcacaactc cgacaagtca
     1321 tggaggcact gacaggcaag gctttttcgg cggccttcgt cacaggacca gcgggagtgg
     1381 gcaaaacaga aatgatgcgc gaaattcaaa gacgcctgtt attgccggaa caggaggcat
     1441 tgtcattggg tgtgccgcga ccactccttg gcaaaaccat atttacgatt gacggaaata
     1501 aatgggatgc caggaacgga caggtaggtg ccgactccaa actcacagcg gagtttattt
     1561 tgtttatgag aagacgtccg gacggcatcg ctttctttga cgaagcgaag atgttgacca
     1621 cctctggtct gtggggtggc ggccaaggtg aaaaagtcaa gggcaattct cccttcgaaa
     1681 aaatgctccc ttatttaagt gaccgcaaat tgtacgccgt ctttgccaca acaacaggcg
     1741 aatacgagga attcttcaag ggtgatccta gatttgccgc cttcctgaga cgcagtgaac
     1801 ctgttgagtt gcagatgttc acgatggacg aagtcaagat tgccttggca acaatttacc
     1861 ccgtactgct tgaaaaactt cgtgttattt ccattgatca aagtgaaccc aaggtggata
     1921 tcgtcgtcaa cgatgaaagc ggaatgaggg tcgtgtctgt tccgcgggta atcagtgacc
     1981 tggtcgattc cacagcaaga gaggcctatg taatagagaa ccccaacgtc ctgatggact
     2041 tctcaatcaa gaggctaaaa atattggtgg ataaagtcac aagagatgtt gttgactaca
     2101 gcacgagacc cgataggggt cgtggcgtcc tcaaacatcc ggtcaatcac gaaatcggca
     2161 ccttgctgga aagacgtgat aaacttaaaa gcgagagaga cagagttaaa tcggaaattg
     2221 agattgccct tgttaagtcg gattcatttt ttcgtgaaat ctacgataaa atacacacaa
     2281 cccccgaact gatcacgaga ctgccgcaga gagtgcaaac cgccatcaaa tccatagtac
     2341 ttggcggaat cggcacgggt gcggtctgta ccagtttgtt tgtaccagag cagattggat
     2401 ggttatccgt ttcaggaatt agcttggggg cgtacatgca aatccggttc ctcaacatcc
     2461 tgtttgggga acaatttagg acttggatca ataatacacc gctcaaagcc cagtttgacc
     2521 gcctttttag aaatcttccc ttttgggaat cagaactcgg acgaatcaat agagagctgg
     2581 agaacatcga ttcgcgtttg ctcgagattg gcggcgaccc a
//
LOCUS       DATDUN010000598         1250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_281011, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000598 DATDUN010000000
VERSION     DATDUN010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281011"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VJC18_08395"
     CDS             complement(<1..1225)
                     /locus_tag="VJC18_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLD45440.1"
                     /translation="MVEIDGMRGLQTSCTIPVKEGMKITSDSVLAREAQKLVINLLLS
                     SGRHDCLACEKNGDCELQTAAYFLGIERPAFTLPEAEIETDDSSEFILRDHSKCIKCG
                     RCVVGCNTTVVNEVLDFGYRGHETRVLCDDDQPMGQSSCVQCGECLQLCPVGALTDKL
                     ARGQGRSWQLKKSETVCPYCGVGCKLNLHIDPVKNKIVRITGVEESPVNNGMLCVKGR
                     YGFDFVGSSERLTTPLIKENGSFRKASWDEAVAWVAKKFGDIKQKNGPDSLGGFASAK
                     CSNEENFIFQKFMRSVIGTNNVDHCARLCHASTVAGLAQAFGSGAMTNDIKGIKEADV
                     IMIIGSNTTEGHPVIGSVIKQAVRLGKTKLIVFDPKRIPLSDYAEIYAAQRCGSDVAL
                     LNGLMHVIIKEELYDK"
BASE COUNT          298 a          323 c          317 g          312 t
ORIGIN      
        1 ttttgtcata gagttcttct ttgatgatca catgcatcag accattgagc agggccacat
       61 ccgagccgca tcgttgggcg gcatagattt cggcgtaatc agaaaggggg atacgtttgg
      121 gatcaaaaac aatcagtttt gttttgccga gtctgacagc ctgtttgatg acactgccaa
      181 tcacgggatg cccctcagtg gtattggatc cgatgatcat gatcacatcg gcctccttaa
      241 tacccttgat gtcgttggtc atggcgccgc tgccaaaggc ctgggccaag ccagccactg
      301 tggaggcgtg acataaacgc gcgcaatggt ccacgttgtt tgtgccgatg accgagcgca
      361 tgaatttctg aaaaataaaa ttttcttcat tggaacattt tgccgatgcg aaaccgccca
      421 gagaatcggg gccatttttt tgtttgatgt cgccaaattt tttggccacc caggcaaccg
      481 cctcatccca cgaagccttc cggaaactgc cattttcctt gatcaggggg gtagtcaaac
      541 gctccgatga accaacgaaa tcgaaaccgt aacgcccttt cacgcagagc atcccgttat
      601 tgacaggact ttcttcaacc ccagtgattc ggacaatttt gtttttgaca ggatcgatgt
      661 ggagattgag tttgcaaccc acaccacaat aaggacaaac agtctcggat ttcttaagct
      721 gccaggatcg gccctgaccg cgtgccagct tgtctgtgag ggcaccaact gggcataact
      781 gcagacattc cccgcattgt acgcaggacg attgacccat gggctggtca tcgtcacaaa
      841 ggacgcgggt ttcgtgtcca cggtaaccga aatcgagaac ttcgttgacg acagttgtgt
      901 tgcaaccaac cacacagcgt ccgcatttga tgcatttgga atgatcgcga aggatgaact
      961 ccgaagagtc atctgtttcg atctcggctt ctgggagcgt aaaagccggg cgttcaatgc
     1021 caagaaagta ggcggctgtc tgcaattcgc agtcaccatt tttttcgcag gccaggcaat
     1081 cgtgacgccc tgacgacagc agaagattga tgaccagttt ttgcgcttct ctagccagaa
     1141 cggagtcact cgtgattttc atgccttcct tgactgggat ggtgcaggaa gtttgcagac
     1201 cgcgcatgcc gtcgatttcc accacacagg cacggcattt accggcttgg
//
LOCUS       DATDUN010000599         1678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_196103, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000599 DATDUN010000000
VERSION     DATDUN010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196103"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VJC18_08400"
     CDS             complement(<1..1290)
                     /locus_tag="VJC18_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45441.1"
                     /translation="MRNPISLSYGLRSVGSMPSLPTIPSATALADLDSALVWLGQNTF
                     QGSTQLKTPPSLQEAQRAWAIDECRSAASFLESAVIKYPDHSPEIAVKHATLQRAMAT
                     AQQLSAPEVESAMTTTAPAPEYLERYPGKSRLFFFLYEALQNIAEHAGFGVEFRRGFL
                     GLGKPEIIVTAYYSYGLPVLSSILDYLNLTSALYIPVDDILIDYDLRTGTQRRVISVS
                     RRNKSDEDDRPIINVIAKTSFDAYGTIITFKPTADNGVTQNHRSHLLLQDDGDRNEGW
                     LSEYLYRFLADHNPIPDSARAVTRTFELSDSQQLAITATRSPTWWYRGLLHPTFRSLS
                     VKMIQGQATIEEQLAFVGARLLGAQFTKAEGQEQFSAHLASGAIFCSSWRQASRRPGD
                     YMLDVVSAVPFDNGPAMQAYDQVFSRLITGRLLDVDVT"
BASE COUNT          458 a          366 c          411 g          443 t
ORIGIN      
        1 ggtaacatca acatcaagca accttcctgt aatcaggcgc gagaagactt gatcatatgc
       61 ctgcattgcg ggcccgttat caaaaggtac agctgaaacc acatcaagca tgtagtctcc
      121 aggccttcta cttgcttgcc gccaggatga acagaaaatg gctccactgg ccaaatgcgc
      181 agaaaactgt tcctgccctt cagcttttgt gaactgtgca cccaagagcc tcgctcccac
      241 aaaggccagc tgttcttcaa tcgtggcctg tccctgtatc attttcactg acaacgaacg
      301 aaaggtggga tgcagcagtc cacggtacca ccaagttgga gatcgagtgg ctgtgattgc
      361 cagttgctga ctgtcagata attcaaaagt gcgcgttact gcacgagctg aatctggtat
      421 aggattgtgg tcagctaaaa aacgatagag atattccgat agccaacctt catttcgatc
      481 accgtcgtct tgcaggagca aatgcgaacg atgattttgc gtcactccgt tatctgcggt
      541 tggtttgaag gtgatgattg tgccgtaagc atcaaatgag gtttttgcga taacgttgat
      601 tattggtcta tcatcctcat ctgatttatt tctgcgtgat acagagatga ctcttcgttg
      661 cgtgcctgtc cgcagatcat agtcaataag aatgtcatca accggaatat acagagcgct
      721 tgttaaattc agataatcca aaatggatga caagactggc aaaccataac tgtaataagc
      781 tgtgacgata atttctggct tgcccaggcc caaaaagcct ctgcggaatt caacaccaaa
      841 ccctgcgtgt tccgcaatat tctgcaacgc ctcgtagagg aaaaaaaaca agcgggattt
      901 gccggggtag cgttcgagat attctggcgc gggcgcggtg gtagtcattg ctgattctac
      961 ctcaggggct gataattgtt gtgccgtagc cattgccctt tgaagcgtcg catgtttcac
     1021 cgcgatttcg ggactgtggt cagggtattt aatgacagca ctttctaaaa aagaagccgc
     1081 agaacggcat tcatcgatgg cccacgccct ttgggcttct tgaagtgagg ggggtgtttt
     1141 taactgtgtt gacccctgga aagtgttttg cccaagccag actagagcgc tgtcaagatc
     1201 agccagagcc gtggcggaag ggatggttgg taaagacggc atggaaccaa ctgatcgtaa
     1261 accataactt aacgaaatgg ggtttctcat catggtgccc tccaatcaag tgactgcgtg
     1321 ttttgcagca cgatgattta tcgtcgctat gtcacatctg ttgcgtaaaa acacgatgcc
     1381 gcacaaaata tcgtgtccca gcggacacgg tattgtgatg attaaatcaa cctgattttt
     1441 ttgccgatga cttcaatgat cagacagtta ttggaaaatg aaaattcgaa tacacaatag
     1501 gggagatgga atctgtgcaa gtcagtcatc cgtggtgaga tgatattgtt attgataaaa
     1561 aaatttttga cgatcaagtt tgaacagaat caggtaagga ctcttgtttc ggatcgttat
     1621 gaataaagag agggaaccac aacacctcta aggggacacg tttttaggat tattgagt
//
LOCUS       DATDUN010000600         1978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_525367, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000600 DATDUN010000000
VERSION     DATDUN010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525367"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VJC18_08405"
     CDS             complement(<1..376)
                     /locus_tag="VJC18_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLD45442.1"
                     /translation="MTYFQNMPFLDFSQKANRDWIAGTLMSVRSQLPFRVPVVVNGKE
                     VSGLETRQVTNPSLSKEVVSINAMATRKEADEALSACESAKQTWGKWPVGKRVHVLRK
                     AADLMEKYRKELTTIMVLEEAKS"
     gene            complement(486..1889)
                     /locus_tag="VJC18_08410"
     CDS             complement(486..1889)
                     /locus_tag="VJC18_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HLD45443.1"
                     /translation="MTSAATKTNPIDDNMNLEHQIKNIGRTIFEQSQAASKIFKKNFF
                     ADLLMDWAMKRQDIKVEMFRFVDVLPTLMTRSQIVDHMREYFLADDKDFPLAIQAGLK
                     VALSNPITSTVTASTVTQNVKAMASVFITGENSERARKKLEDLWNDNYCFTVDILGEA
                     VVSEPEAREYQGRYLELVKGLAEHVKPWKPNPILEQTSLGKIPRANVSVKLSSLFSQI
                     DPLAFESSVKGIKEALRPILRSAMQNNVFINLDMEQNDFRDVTLTVIEELFTEPEFAD
                     YPHFGLVIQAYLKGAVQDLSRIITLSSGRKTPLTVRLVKGAYWDYEVILARQRNWPIP
                     VFLKKPESDVNYELCTRLLLDAYPKILSAFASHNIRSVSHAMAYAAHLGLNKTDFEIQ
                     TLYGMGNNFKKPIVNMGYRVREYSPIGEMLPGMAYLVRRLLENTSNEGFLKQSTVDLK
                     DVDELLRNPEELVDRES"
BASE COUNT          476 a          444 c          548 g          510 t
ORIGIN      
        1 acgatttggc ttcttcgagg accatgatgg tggtgagttc cttgcggtat ttctccatga
       61 ggtcagccgc cttgcgcagg acgtgtacgc gcttgcccac gggccacttg ccccacgtct
      121 gtttggcgct ttcacaggca gacagagcct catcggcctc tttgcgggtg gccatggcgt
      181 tgatgctcac gacctctttt gacagtgagg ggttggtgac ttgtctggtt tcgagcccac
      241 tcacttcttt gccattgacc acaacgggga ccctgaaggg gagttgcgaa cgcactgaca
      301 tgagcgtgcc agcgatccaa tcacgattgg ctttttgaga aaaatcaaga aagggcatgt
      361 tttgaaaata cgtcatatta tcgcgtgaat ggtcactcgt cggccgtgta ccgaaaataa
      421 atttcgattc acgattcata ccctaaaggg cacaggcgat tcatacccta aagggcacag
      481 gcgattcacg attcacgatc tactaattcc tccgggttac gtaacagttc gtcgacatct
      541 ttgaggtcaa cggtactttg ttttaaaaag ccttcattcg atgtgttctc caagagccgc
      601 ctgaccagat aagccatgcc aggcaacatt tcgccaatgg gggaatattc gcgtaccctg
      661 tatcccatgt tgacaatggg ttttttaaaa ttattgccca taccgtaaag cgtttgaatt
      721 tcgaaatcgg ttttgttcag ccccaaatga gcggcataag ccatggcgtg ggaaacagag
      781 cggatgttgt gcgaggcaaa cgcgcttaag atcttagggt aggcatccag caacaagcgg
      841 gtgcagagtt cgtagttgac gtcggactcg ggttttttca gaaaaacagg gatgggccag
      901 ttgcgttggc gggccagaat cacctcatag tcccaatagg cccctttgac caaacgcacg
      961 gtcaacggtg ttttgcggcc ggaagacagt gtgatgatgc gtgagagatc ctgcacagca
     1021 cctttcaaat aggcctgaat gaccagtcca aaatgagggt aatcagcgaa ttcgggttcg
     1081 gtaaaaagtt cctcaatcac ggtgagggtg acgtcacgaa aatcgttttg ctccatgtcg
     1141 aggttgatga agacgttgtt ttgcatggcc gagcgcaaaa tggggcgtag ggcttctttg
     1201 atgcccttga cggaagattc aaaggcgagc ggatcaatct gcgagaagag acttgataat
     1261 ttgaccgaca cattggcgcg cgggattttg cccaaagagg tttgttccaa aatgggatta
     1321 ggcttccagg gcttgacgtg ctctgcgagc cctttgacga gttcgagata gcggccctga
     1381 tattccctgg cctcgggctc ggagaccacg gcctcgccca ggatgtccac ggtgaaacag
     1441 taattgtcgt tccagaggtc ttctagtttt ttacgggccc gctcggaatt ctctccggtg
     1501 atgaacacgc tggccatggc tttgacgttc tgcgtgacgg ttgaggcggt gactgtggag
     1561 gtgatgggat tggacagggc caccttcagc cccgcctgga tagccagcgg gaagtctttg
     1621 tcatcggcca gaaaatactc tctcatgtga tcgacgatct gtgatctggt cataagggtg
     1681 gggagcacgt caacaaaacg gaacatctcg accttgatgt cctgacgttt catggcccaa
     1741 tccatgagga gatcggcaaa gaagtttttt ttgaaaattt tagaggcggc ctgactctgt
     1801 tcgaagatgg tacggccaat gtttttgatt tgatgttcga ggttcatatt atcatcgata
     1861 ggattggttt ttgttgcggc ggaagtcatg gtggaaaatc tctatggggg aataaatcat
     1921 tttgcaaatg gaaaaatagt ggaaaaatag tggcgagaca gaagttctta aataataa
//
LOCUS       DATDUN010000601         1551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_400263, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000601 DATDUN010000000
VERSION     DATDUN010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400263"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VJC18_08415"
     CDS             <1..335
                     /locus_tag="VJC18_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45444.1"
                     /translation="KVDAKGLLVLDEASKKALQELVETYPSPRVQLDREIKRIKNAQE
                     KELIGNQVYRDRSGFSVGEKINETTTASLQHLEGIKSSLPPATSEPIPEKSFDIKNLR
                     KPKGKVFG"
     gene            complement(515..>1551)
                     /locus_tag="VJC18_08420"
     CDS             complement(515..>1551)
                     /locus_tag="VJC18_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HLD45445.1"
                     /translation="EVPSLVAGQSRTKGTGEAVDAKPKFVVPYSPNDDYAKFKPLPFI
                     DGLRKAQALDLPDPDMQEDDKNLSNLSYDKRVGSLILNGIEGDDVHQGQVGDCYFLAA
                     LSSLAETHPEFLEKNIQKNCDGTYTVGFHKITHPDGCIKYDEDGAYTLGIRTTKKITV
                     DTELPKYGTQNLYARGESNNELWVALYEKAYAVYKGNDYDAIHRGNPAQAFYELVGVT
                     SKTTTTSSCDADELYECLATAVKEGNPIVASSNDSADGTLVTAQNILCDHAYSVLGVK
                     ERNGTKYVQLRNPWGYKEWTGDGSDAVDDGTFWMPLTDFKSDFHCVNVLDDQGYENLY
                     EPHSHPKKRV"
BASE COUNT          370 a          418 c          359 g          404 t
ORIGIN      
        1 gcaaggttga cgccaagggc ctgctggttc tggacgaggc atcaaaaaag gccctgcaag
       61 aactggtcga aacctaccct tccccgcgcg tgcaactcga tcgcgaaatc aaacgaatca
      121 agaacgcgca ggaaaaagaa ctcatcggca atcaggtcta tcgtgatcgc agtgggttca
      181 gtgtgggaga aaaaatcaac gagaccacca cagcctcatt gcaacatttg gaaggcatca
      241 aatccagcct tccccccgcc acttccgaac caattccgga aaaatccttc gatatcaaaa
      301 atctccgcaa acccaaggga aaggtgtttg gatgattaat acgtgaagcg tgaagcgtaa
      361 agcgtgaagc gtcccgcaat gcggaatcag tcctgaggct gacatttttt attttgcact
      421 tttcactttt cattttgcat tgttcacttc ccaaatcccc ccgtccctcg tccctcgtgt
      481 tttcgtgttt tcttgttttc gttccccgtt cccctcacac cctcttcttc gggtgactat
      541 gcggttcata cagattttca taaccttgat cgtccagcac attcacacag tgaaaatcac
      601 ttttgaaatc agtcagaggc atccagaagg tgccgtcgtc aacagcgtca cttccatccc
      661 ccgtccattc cttgtaaccc caagggttac ggagctgaac atatttggtg ccatttcgtt
      721 ccttcacgcc cagcacggaa tacgcgtgat cgcagagaat attctgagct gtcactaagg
      781 tcccatccgc gctgtcattt gaagaggcca cgatagggtt gccttccttt acagcagttg
      841 cgagacattc gtacagttca tccgcgtcgc acgaagatgt ggtggttgtt ttagacgtca
      901 caccaaccag ctcatagaag gcctgtgcgg gattaccacg atgaatagca tcgtaatcgt
      961 tgcccttgta caccgcgtag gccttttcat acagggctac ccaaagctca ttgttggact
     1021 ctcctctggc atacagattc tgtgtcccat acttgggaag ttccgtgtcg accgtgattt
     1081 tttttgttgt gcgaataccc agtgtgtagg caccgtcttc atcatattta atgcatccgt
     1141 cgggatgggt aattttgtga aagccaacgg tgtaggtgcc atcgcaattt ttttggatgt
     1201 ttttttcgag aaattcggga tgtgtctcgg ccagcgatga cagagcggcc agaaaataac
     1261 aatctcccac ctgcccctga tgcacatcgt caccctcgat tccattaagg atcaacgaac
     1321 caacgcgctt gtcgtaagac aggttggaca gattcttgtc gtcttcctgc atgtccggat
     1381 cgggcaaatc cagagcctga gccttacgca ggccatcaat gaaaggcagg ggtttgaact
     1441 tggcatagtc atcgttgggg gaatacggca caacaaactt tggttttgca tcgaccgcct
     1501 cccctgtacc tttggtcctg gattgcccgg ccacgagcga ggggacttct c
//
LOCUS       DATDUN010000602         5301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_110932, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000602 DATDUN010000000
VERSION     DATDUN010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110932"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VJC18_08425"
     CDS             <1..244
                     /locus_tag="VJC18_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45446.1"
                     /translation="HHFRGPLKSAQMQGARKPANGAYTEVREYCGCSGQHRRWVVFRG
                     PYNNHLSSYAEIDIVLEWETLFSINYQKSLQPLLCL"
     gene            235..519
                     /locus_tag="VJC18_08430"
     CDS             235..519
                     /locus_tag="VJC18_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLD45447.1"
                     /translation="MSVNPKKIFLFGSFAMGSATPDSDLDLLIITGNAITSFKIRRKI
                     ISVIRSVLSRFRIPKDILVYDETELNKWSKSPNHIIGRALAEGKLLYERP"
     gene            506..898
                     /locus_tag="VJC18_08435"
     CDS             506..898
                     /locus_tag="VJC18_08435"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLD45448.1"
                     /translation="MKDLEYAQTMLTQANKDFKALLNMLDETLFEPEIFGFHAQQTIE
                     KALKAWLSFLGVVCPKTHDLEMLFALLEDNKVPIPDQFRSLTDFTDFAVQFRYEAFTD
                     FSDDDFDRQNIVSTIGAFIQYVKEQTGG"
     gene            1331..2530
                     /locus_tag="VJC18_08440"
     CDS             1331..2530
                     /locus_tag="VJC18_08440"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45449.1"
                     /translation="MLESFPVVIILGVRQCGKTTLAKQVRPDWYFLDLEKSSDFRKLV
                     DDFDFFFKEHPQHVIIDEAQQSPQLFEELKAVIDNDRSQKNRFLLTGSSSPLLIKNVS
                     ETLAGRVGIVDLGTFKINEIYKKPLSDFYKIFESLLGQNTQDQIFSLKTALTHDHVMN
                     AFLKGGYPEPVLANNNTTYRLWMHNYYQTYFQRDLRALFPRLDFVKYQRFITMLSALT
                     GTIINKSEVGSSLDTSEVTVRDYLDIAQGSFFWRSILSFENSVSQSIVKKPKGNFRDS
                     GLAHYLQNVQTREELINHHHVGANFEAFVTEEIIKGLDATLVTGWNYFFYRTKHGAEV
                     DLVLTGPFGVLPIEIKFGMNTSPRRLVSLKEFIAKNNYPLALVINNADKIERLASNII
                     QLPATFL"
     gene            2751..2993
                     /locus_tag="VJC18_08445"
     CDS             2751..2993
                     /locus_tag="VJC18_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020861352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HLD45450.1"
                     /translation="MSPTVLRIKGYRFYFLSNEEDRMHVHITCANGEAKFWLEPIVAL
                     ATYYKLNAKNLNELQKLVERHKNEFIKEWKKYFSVR"
     gene            2950..3249
                     /locus_tag="VJC18_08450"
     CDS             2950..3249
                     /locus_tag="VJC18_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HLD45451.1"
                     /translation="MNSSKNGKNISVSVENVSSLGIWLFVKGKEYFLSFNDYPYFQHQ
                     TLKAIQNVQLCHNVHLYWPELDIDLEIDNLEYPEKYPLKCRLTKSSSQLNLKRSA"
     gene            3479..3748
                     /locus_tag="VJC18_08455"
     CDS             3479..3748
                     /locus_tag="VJC18_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HLD45452.1"
                     /translation="MPILSAFYGLVVMMFFLDNKKHSKPHVHVKYQDEMAVIGIPNGE
                     VIEGSLPVNRLKLVQAWIEIHKEELMTDWSLAVAGQEIFKIEPLR"
     gene            3754..3990
                     /locus_tag="VJC18_08460"
     CDS             3754..3990
                     /locus_tag="VJC18_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HLD45453.1"
                     /translation="MLEVIESKPNPDFTIILTFDNNERRVFDLKPYLVIGVFKQLQKP
                     ECFITVKPFFGGIAWDTGQDLSPETLYIESKPLT"
     gene            4407..>5301
                     /locus_tag="VJC18_08465"
     CDS             4407..>5301
                     /locus_tag="VJC18_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45454.1"
                     /translation="MVQGRIDNSFAQLPDPESDLTELKQNETLDELAQLINVEEVPVP
                     GKPGFVMTQIDLASGNYTPEDATDKATTLNKGTIRIGFWSTNAKTFLGHSGVSGYEKD
                     SETHKYKSTVKGEKTKDVLKSGFGLEQHKVWLKLETNDTIIKYKAPGDASFTEITKDS
                     VGITPFTKKNEKTDVMEETTVWNEIYKLIKDFDNIRSDDGQGIVKATYDVHMSSYYKA
                     LTDCAAGKFDDKGISLSKYIDPPLKNKEGKKIMLNAMLTMACLGFFKDATYVDMARGI
                     VKFADQEKSRAALAKICGVGLA"
BASE COUNT         1530 a         1308 c         1073 g         1390 t
ORIGIN      
        1 ccaccatttt cggggacctc taaaaagtgc ccagatgcaa ggcgcccgca aacccgcaaa
       61 cggagcgtac acggaagtac gtgagtattg cgggtgcagt gggcaacacc gcagatgggt
      121 agtttttaga gggccctaca ataatcattt gtctagttac gccgaaattg atattgtcct
      181 ggaatgggaa acgttgtttt caatcaatta tcagaaatcg ttacagccat tattgtgtct
      241 gtaaacccaa agaagatttt cttgtttggg tcttttgcaa tgggcagtgc tacacccgat
      301 tccgatttag atctgctgat catcactggg aacgcaatca ccagtttcaa aatccgccga
      361 aaaatcatct cggttatccg aagtgtgttg tctcgtttta gaattccaaa agatattcta
      421 gtgtatgacg aaaccgaatt aaataaatgg tccaaatcac ctaatcacat tattggacga
      481 gccctggccg aagggaagct actctatgaa agaccttgaa tacgcacaaa caatgctcac
      541 acaggcaaac aaggatttca aagccctgct taacatgctg gacgaaacgc tttttgaacc
      601 cgaaatattc ggttttcatg cccaacagac aatcgaaaaa gcactcaagg cctggctgag
      661 tttcttgggt gtcgtttgcc caaaaacaca tgatctggaa atgctttttg cattactcga
      721 agacaacaaa gtacccatac cggatcagtt tcggtctctg actgatttca cggatttcgc
      781 cgtgcaattt cgttacgagg ccttcacaga tttttccgac gatgatttcg acagacaaaa
      841 catcgtttct acaattggtg ccttcattca atatgtaaaa gaacaaacag gcggttagtg
      901 ctttggctgt tattttttac ccacgctccc cttccccatt gcccattgcc atctcgtagg
      961 ggcgagtctc gactcgccct gtattgctct gccttattga ttacgcacaa ttatcaccat
     1021 aaggtgccag tcaacaatcg aacaaaaaag tgccaccctg taccgataac tggtacacag
     1081 gccattgcgg caattgccgg ttgcaacaat tgcgcaatta tccccctccc ccctttgacc
     1141 cattgaccct ttgacccttt gaccatttga cccattcgct tttcgccccc tgccctgagc
     1201 ctgtcgaagg gttccccctt atgcctgacg ccttttcctg aacgtttttg ctctttacac
     1261 cggtaaattt ctagtgtaga gttagaaaat ggccggtatc aaaagaaatt tatttaacaa
     1321 aatcaacagg ttgttagaat cattcccagt agtcataatc cttggcgtgc gccagtgtgg
     1381 caagacgacc ttggcaaaac aagtcagacc agattggtat tttcttgatc tggaaaaatc
     1441 atctgacttt agaaaacttg ttgatgactt cgattttttc tttaaagaac accctcaaca
     1501 cgtcatcatc gatgaagccc agcaatcacc ccaactcttc gaagaattga aggcagtgat
     1561 agacaatgac cgttcgcaaa aaaacagatt tttgctcacc ggttcaagct cccctctttt
     1621 aatcaaaaat gtttccgaaa ccctggctgg cagagtgggc attgtcgatt tggggacttt
     1681 taaaattaac gagatataca aaaaacctct ttctgatttt tacaaaattt ttgaatctct
     1741 tttgggccaa aacacacaag atcaaatttt ctcactcaaa acagcgctga cacacgatca
     1801 cgtcatgaac gcctttttga aaggtggcta tcccgaaccc gtgctcgcaa acaacaacac
     1861 cacctaccga ctctggatgc acaattatta tcaaacctat tttcaaagag accttcgcgc
     1921 gctgttcccc agactggatt ttgtgaaata tcaacgtttc atcaccatgt tatcggcttt
     1981 aacgggcacg atcatcaaca aatccgaagt gggtagttcg ctcgacacct ccgaggtgac
     2041 tgttcgcgac tatctggaca tcgctcaagg aagttttttt tggcgaagca ttttgagctt
     2101 tgaaaactct gtgagtcaat caattgtaaa aaaaccaaag ggcaatttca gggattccgg
     2161 attggcacat tatctgcaaa acgtacagac cagagaagaa ctcatcaatc atcaccacgt
     2221 cggagccaat tttgaggcgt tcgtaaccga agaaattata aaaggcctgg acgcgactct
     2281 cgtgacaggt tggaattatt ttttctacag aacaaaacac ggagcagaag tcgatttggt
     2341 cttgaccggc cctttcggcg tgcttcccat agaaatcaag tttggcatga acacttcgcc
     2401 aagacggttg gtttctctaa aagaattcat tgcgaaaaac aattatcccc tggccttggt
     2461 catcaacaac gcagataaaa tcgaaagact tgccagcaac atcattcaac ttccggcgac
     2521 tttcttgtaa cgtcccccat tgcccattgc tacaattgca ccattgcccc cctccccccc
     2581 ttacgcctta cgccctaagc cttgcgcctt acgcccacgt aaacacatga acacttgaac
     2641 ccgtccccca tcccccatga ctcgttacgt cgctaccaat catgtcaatt gatcattgat
     2701 aatgtccacg atgatctgct actatgtttg cggggggtag cgtaaataac atgagtccta
     2761 ccgtattaag aatcaaggga taccgattct attttctgtc taatgaagaa gataggatgc
     2821 atgtgcacat cacttgtgcc aatggggaag caaaattttg gttggaacca atcgttgcac
     2881 ttgccacgta ctacaaactg aatgccaaaa atttgaacga actacaaaag cttgtcgaaa
     2941 ggcataaaaa tgaattcatc aaagaatgga aaaaatattt cagtgtccgt tgagaatgtt
     3001 tcctctctgg gaatctggct tttcgtcaaa ggcaaagagt atttcttaag ctttaacgat
     3061 tatccctatt ttcagcacca aacactgaag gcaattcaga acgttcagtt atgccacaat
     3121 gtccacttgt attggccaga attggatatt gatttagaaa tcgacaatct ggaatatccc
     3181 gaaaaatatc ccctgaaatg tcgactgacc aaaagcagtt ctcagcttaa tttgaaacga
     3241 tccgcataag gtgccagtcc ccgcaaagct gagccacggg gcaagttccc attgctcatt
     3301 gcaaattgct ataattgcac cattgcccat tgctacaatt gcaccattgc ctccgccttg
     3361 cgcctctttt ggtgcacagc gctcgaattg cccctggcaa ttcgagcgct gtgcaccccc
     3421 aacatgaaca cttgaacaaa taatgtgact ttttcccgaa tatttataat acttagctat
     3481 gcctatcctt tctgcgtttt atggcttggt ggttatgatg ttttttttag ataataaaaa
     3541 gcacagcaaa ccacatgtcc acgtcaaata tcaggacgaa atggccgtca tcggcattcc
     3601 caatggcgag gtgattgagg gttcgttacc agtcaatagg ttaaaacttg tacaagcctg
     3661 gattgaaatc cacaaggaag aactcatgac cgattggtct ctggcggttg ccggtcaaga
     3721 aatttttaaa attgaaccgt tgaggtaacc caaatgttag aagtcatcga atcaaaaccc
     3781 aacccagatt ttacgattat tctgactttc gacaataacg agcgacgcgt ttttgattta
     3841 aaaccctatt tggtcattgg ggtttttaaa caattacaaa aaccagagtg tttcatcaca
     3901 gtaaaaccgt tctttggcgg gattgcctgg gatacaggcc aagacctctc tcccgaaaca
     3961 ctttacatcg aatcaaaacc cctgacataa ggtgccagtc cccgcaaagc tgagccacgg
     4021 ggcagttccc attgctcatt gctacaattg ccggttgcaa caattgcgca attgcccccc
     4081 tccccccttt gaccctttga ccttttgacc ctttgaccca ttcgcttttc gccccctgcc
     4141 ctgagcctgt cgaagggtta ccccttatgc ctgacgcctt acgccctacg taaacacctg
     4201 aacacgcctg ccccctgcgg gggtgaacac gttctcccta taaatcccac gacaacagtt
     4261 aactctcgtg atcaaactgc cgaaaatagt gattgtcagt cacttttcat ttgggggcag
     4321 tccaaaaata gtatacaata ttaaatggac accattggtg accggtttac aaacggcgca
     4381 cttacggtct cctggaaggg ttgtttatgg ttcagggtcg aatcgacaat tcatttgcgc
     4441 aactgcctga tcccgaaagt gatctcaccg aactcaaaca aaacgaaaca ctggacgaac
     4501 ttgcccagtt gatcaatgtc gaagaagtcc ccgtacccgg caaacccgga tttgtaatga
     4561 cccagattga cctggcatcc ggcaactaca cgccggaaga cgccaccgac aaagccacca
     4621 cgctcaacaa gggcactatt cgaattggtt tttggtctac aaacgccaaa acctttttgg
     4681 gtcattccgg ggtgagtggg tatgaaaaag attcggaaac tcataaatat aaatctaccg
     4741 ttaaaggcga aaaaacaaaa gacgtcttaa aaagtggttt tggcctcgag caacacaagg
     4801 tgtggctcaa gcttgaaacc aatgatacca tcatcaaata caaggccccg ggggatgcca
     4861 gttttaccga aatcaccaag gacagtgtgg gaatcacccc ttttacgaag aaaaacgaga
     4921 agaccgacgt tatggaggag accaccgttt ggaatgaaat ttataaacta ataaaggact
     4981 ttgacaacat tcgtagtgat gacggccagg ggatcgtcaa agccacatat gatgtacaca
     5041 tgagctctta ctataaggcc ttgactgatt gtgccgcagg taaatttgat gacaagggca
     5101 ttagtctttc aaaatacatt gatcctccgc taaaaaataa ggagggcaag aagatcatgc
     5161 tgaacgccat gctgaccatg gcctgtctgg gttttttcaa agacgcgaca tacgtcgaca
     5221 tggccagagg gatcgttaaa tttgctgacc aggaaaaatc tcgggccgcc ctcgccaaaa
     5281 tctgtggcgt tggtctggca a
//
LOCUS       DATDUN010000603         1639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_110969, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000603 DATDUN010000000
VERSION     DATDUN010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110969"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VJC18_08470"
     CDS             <1..821
                     /locus_tag="VJC18_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45455.1"
                     /translation="GDKEVHMEMEHVPTADSYVYSGDFFIMINESGYGGNCPDSDVTN
                     NFTVAYEMTSKTSAALQARSAMFCGSDIDGRDANGYVAAGDKYSDENPDGWGNDYNLL
                     TANFDPTTLDGNYSYAWQAGHFDNNTRVFNIIVTDEGTQGTSFYGYGDDIAATDGSID
                     GFICNWVAPGADHTMLEYVQQQDMSVDLETGLVSAEASFITYAPTTTCEYDGTGAFVF
                     DTNIDDDLSDEDAALVITTDAGTMDLVPGADENGDSEATVEEVIEASGFTLPAM"
     gene            complement(882..1244)
                     /locus_tag="VJC18_08475"
     CDS             complement(882..1244)
                     /locus_tag="VJC18_08475"
                     /inference="COORDINATES: protein motif:HMM:NF020360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HLD45456.1"
                     /translation="MNNNLLLKSLKELETALASQTIDDIVFDAICKRFEVCFEYVWKF
                     FKQTATSAGVEVYSPKDAIKEAVKMKLINDLDLWLDFLQERNLSVHNYYSGDPTETLK
                     KIRAFAKAVRKIKDDPHH"
     gene            complement(1237..>1639)
                     /locus_tag="VJC18_08480"
     CDS             complement(1237..>1639)
                     /locus_tag="VJC18_08480"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLD45457.1"
                     /translation="CQTLKVSPSSIIEKSDTVSCLHPDIAKAVDQLHTTHPHLTFVLF
                     GSRSKGLQKKYSDWDIGFFSMTTAITHQTHLDLLKQVDDLMEDSLETIDLVNLNLADH
                     AFLSDNKYDFIFLTGCLTDWRLLQHKLLSYE"
BASE COUNT          431 a          363 c          438 g          407 t
ORIGIN      
        1 gcggtgacaa agaggttcac atggaaatgg agcacgtgcc cacggccgac tcgtacgtgt
       61 actcgggtga tttcttcatc atgataaacg aatctggtta cgggggcaat tgtccggata
      121 gcgatgtaac caacaacttc accgttgcct acgaaatgac ctccaaaacg tcagcggcct
      181 tgcaggcgcg tagtgccatg ttctgtggtt cggacattga tggcagggat gccaatggct
      241 atgtagccgc tggggataag tattctgatg aaaatcccga tggctggggc aacgattaca
      301 atctgctcac agcgaatttt gaccccacaa cactggacgg caattattca tacgcgtggc
      361 aggccgggca tttcgacaac aacacgcgcg tgttcaacat catcgtgacg gatgaaggaa
      421 cacaggggac atcattttac ggatacgggg atgacattgc cgcaaccgat ggaagcatcg
      481 atgggtttat ctgcaactgg gtagccccgg gcgcggatca caccatgttg gagtacgtgc
      541 aacagcagga catgagtgtt gatctcgaaa ctggtttagt cagtgcggag gcgtcgttca
      601 tcacttacgc gcccacgacg acttgcgagt acgatggcac gggtgcattc gtgttcgaca
      661 cgaacatcga tgatgacctg agtgatgagg acgcggccct ggtcatcacg acggatgctg
      721 gtaccatgga tctggtgccg ggtgcggatg aaaatggaga cagcgaggcc accgtggaag
      781 aggtgattga ggcctccggc ttcaccctcc ctgcgatgta aacaaatagt gttggaaaag
      841 ataatctcac aaggcggatc actcagatcc gcctttttta attaatggtg cggatcatcc
      901 ttgattttcc tgactgcctt tgcaaaggcc ctgatttttt tgagcgtctc tgtgggatca
      961 ccggaataat aattgtgcac cgaaagattt ctttcctgca gaaaatcgag ccacaaatcc
     1021 agatcattga tcagcttcat tttgacagct tccttgatgg catcttttgg actgtaaacc
     1081 tccacaccag cgctggtggc agtttgcttg aaaaacttcc agacatattc aaaacaaact
     1141 tcaaaacgtt tacagatggc atcaaaaaca atgtcatcaa tcgtttgaga ggcgagcgct
     1201 gtttcgagct ctttgaggga cttaaggagc aggttgttat tcatatgata aaagcttgtg
     1261 ttggagcaga cgccaatcag taaggcatcc tgttaaaaaa ataaagtcgt atttattgtc
     1321 tgacaaaaaa gcatgatcag cgaggttgag gttgacgagg tcaatggttt ccagcgagtc
     1381 ctccatgaga tcatcaactt gtttgagcaa atcgagatgt gtttggtggg tgatggcggt
     1441 tgtcatggaa aaaaaaccga tatcccaatc tgaatatttt ttttgcaatc ctttggaacg
     1501 tgaaccaaac agcacaaagg tcaggtgggg gtgcgtggtg tgcagttgat ccacggcctt
     1561 cgcaatatcg ggatgcaaac aggaaaccgt gtcgcttttc tcaattatcg atgaggggga
     1621 caccttgagt gtctggcac
//
LOCUS       DATDUN010000604         6406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_442954, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000604 DATDUN010000000
VERSION     DATDUN010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442954"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..473)
                     /locus_tag="VJC18_08485"
     CDS             complement(45..473)
                     /locus_tag="VJC18_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45458.1"
                     /translation="MFKWVKNNAGFSLLEATLAVVIISTGMLAGLSVLQSNVVASVSM
                     ERNTIATQLANEKMEVILADNKYRGYDYATNTVNYPAEELAGSFNGFTREVEIVEVNT
                     DDLTTPAVGSGLNKVVVTVSWADNEDSQVQISTLIANADY"
     gene            746..2470
                     /locus_tag="VJC18_08490"
     CDS             746..2470
                     /locus_tag="VJC18_08490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HLD45459.1"
                     /translation="MYKPTNFIPANLEISEWSDMQPYWDKLLAVDPATVQELEDLILH
                     HSEVLSVFYEQYAWAYINMTRHTDHEDKQKRFEHFATVILPEVEKAGNTVQQKITTSP
                     YFKALPPERYAQLQREFMRDVELFRENNVALEAKLSTLATEYSQIISQLTVVLEGHEM
                     TIQQASVFLESPDRAKRKTAWLAIMQKRLGIKTQLDDIMNEMIGLRHKIAQNVSYANY
                     RDFKHDALHRFDYSVQDVLCFHDAIKRCVVPLLNKIENRQRQRLRLNPDDYWPWDVSG
                     KTIDEIPLKPFEQVADLLDKTIRVQKLICGAFADNLEHMRQAGFFDLETRPHKAPGGY
                     NYGLEMTGMPFIFMNAAGMHTDVTTLMHESGHAMHTFCTHNEPLIQYRNCPAEVSEVA
                     SMSMELISSHHWHEFYSSQDVLRARIDHLEDIVSTFAWVAVVDSFQHWLYTHPTHTVA
                     QRSAQFSLIAAQYESGLVSWDGYESFKENMWQKQLHIFEVPFYYIEYAIAQLGALQIF
                     ANYQKDPQRTIEAYIAGLRLGSSRLLQEVWKTLNIKFDFSEANLKSLMAFVEREWSTL
                     CAEYDVVN"
     gene            complement(2492..2908)
                     /locus_tag="VJC18_08495"
     CDS             complement(2492..2908)
                     /locus_tag="VJC18_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLD45460.1"
                     /translation="MNSKLNCKGFSLLEAVLAMTILAIGLVTGLSVLSNVVATSVDQE
                     IHIIASQKASEQIEIILADRQFRGYDYVTTNNYPAEVLSGSYNLTKNVTITEVSSSDL
                     VTPQINSGAKKVDVQVSWGTGAYQQVLLTTLVVDYE"
     gene            complement(2931..3149)
                     /locus_tag="VJC18_08500"
     CDS             complement(2931..3149)
                     /locus_tag="VJC18_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45461.1"
                     /translation="MKQWRNNQGSFCLYSIMILMLLGFLFSVSFFGVATVRSFIDFEH
                     AKGLGVALHNEKVEEIENLLDDVLSANG"
     gene            complement(3383..4198)
                     /locus_tag="VJC18_08505"
     CDS             complement(3383..4198)
                     /locus_tag="VJC18_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLD45462.1"
                     /translation="MKSLASCVSSYYRDYRRLEIMNMAASLSFYTLIAIFPTILLLAT
                     IAGSFISQDLIQDSLTKLIHETLPYQSDLLMQNMWALIEKKQTFGWFSLVVLAFSAQM
                     LFVNFERIINHILQAKKTRHFLVTHLISFAWLLGLVFILFTPVIIEMISVRITSLNEQ
                     WHQYANFFASGGFVLLGCVLFWVIMQTLAAKKNNPKRVLWGALFFSLTLQLGKYLFKF
                     FTLRQLDRYNLVYGSLSSIVLTTLWILYFYNIFLFFVYWTGHQQSSTRVKKVK"
     gene            complement(4216..5631)
                     /locus_tag="VJC18_08510"
     CDS             complement(4216..5631)
                     /locus_tag="VJC18_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45463.1"
                     /translation="MLNFTAKCVFLALVIISLPAYAAITLTGDVDADFTNENCLIDAG
                     GQDIGLVGGISGTGFDIDKVCFYYDGTNDSLSIGISTINKVIFGDTDGDGDPGSSSHG
                     GVVDYPNLGTGETIAIAVDLDGDSATSGFNESTVDALIGVAPAATLDGLGVYDVSVGF
                     DTLTPAAGFGSTELAQTVLYATPSTSVRDLEFEIKEFKGIQTSGMASIINNVYLLIFA
                     DALVIGEDYLPDINESQIHAIFDHDEDLLEDWDEMDNTLTDPNDADSDDDELLDGTEV
                     NGDNPTDPLNPDTDGDTCLDGIEDANHNGAYEPFLNEADPNQIDTDADKIDDCTELTG
                     TNPTNPSAADTDGDGLLDGDEDKNQNGAFEPTLGETDPNKPDTDGGGVDDKTEIDNGF
                     NPNDPTDDQQASEQINVTTYNQAQGGGCSLSQFSTGNKQQATVSLRSTIYDPRSTWLF
                     VVGLCVLCTLRFLLTRKTVFR"
     gene            complement(5830..>6406)
                     /gene="selD"
                     /locus_tag="VJC18_08515"
     CDS             complement(5830..>6406)
                     /gene="selD"
                     /locus_tag="VJC18_08515"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HLD45464.1"
                     /translation="NKLITNAGAKPGDTLILTKPLGTGTLMSALKKGVKSESELIEAT
                     TSMCTLNNIHDCLQSEDWQYLHAMTDVTGFGLAGHAYQLAKASNVTLTITSAALPRFE
                     HVYETLKTECLTKAHGTNRQYVKDHLIIENTVSDLDQLVFFDPQTSGGLLLSVDEKHA
                     PTLLHKLKAPFPKACVIGQVTEKGDHTLILL"
BASE COUNT         1674 a         1447 c         1635 g         1650 t
ORIGIN      
        1 tggcgtttaa cgaaaaacgc attcttcccc cctcgcatta aatattagta gtcggcattc
       61 gcgatgagtg tgctgatctg gacctggctg tcctcgttgt ctgcccaact cactgtcaca
      121 accactttgt tgagcccaga accaacggcc ggagttgtca aatcgtcggt gttgacctcc
      181 acgatttcga cctctcttgt gaagccgttg aaggaccctg ccaattcttc tgctgggtaa
      241 ttgacggtat tggtggcgta atcatacccg cgatacttgt tgtcagctaa aatgacctcc
      301 attttttcgt tggccagctg agtggcaatg gtatttcgtt ccatactgac gctggccacg
      361 acgttgcttt ggaggaccga caaaccggcc aacatcccgg tcgatatgat gacgactgcc
      421 agcgttgctt ctagtagaga aaaaccagca ttgtttttta cccatttaaa cataaccctc
      481 aaaccccctc atccacattc atccaagcaa gttctgtgcc agacaggaag aaaacataat
      541 cagctgtaat tgttcactaa tattattggt gacaggggac gagatcacaa ttgatgacgt
      601 tttctgtctt tattacccaa ggcgtgtcac agtttgttac gaaaaagcaa agagacaaca
      661 atggggagca ggacactgta aagaccttct gtgtttcgaa atttcccgtt cgatatttgg
      721 tctgaatata tgctacgggg aattcatgta caaacccaca aattttattc ctgcgaatct
      781 agaaattagt gaatggtcag acatgcaacc ttattgggac aagctattgg cagtagatcc
      841 cgcaacggtt caagagctcg aggatttaat cctgcatcat agtgaggtgt tgagtgtgtt
      901 ttatgaacag tatgcctggg cgtacattaa catgacgcgg cataccgacc atgaagacaa
      961 acaaaaacgg tttgaacatt tcgcgacggt gatcttgccc gaagtggaga aggcaggaaa
     1021 cactgtccag caaaaaatca ccacaagccc ttatttcaag gctttgccgc cagaacgtta
     1081 cgcccaatta cagcgtgaat ttatgcgcga tgtggaattg ttcagggaga ataatgtggc
     1141 tttggaagca aagttgagca cattggccac ggagtacagt caaatcatca gtcaactgac
     1201 agttgttctc gagggacacg agatgaccat acaacaagcc tctgtgttcc tggagtctcc
     1261 tgatcgggcc aaaagaaaaa cagcctggct ggccatcatg caaaaaagac ttggcatcaa
     1321 aacacaactt gatgacatca tgaacgagat gattggcctg cgtcacaaga ttgcgcaaaa
     1381 tgtgtcctat gccaattatc gcgatttcaa acacgatgct ttgcatcgtt tcgattattc
     1441 tgtgcaggat gtgctgtgtt ttcatgatgc gatcaaaaga tgtgtcgttc ctcttctgaa
     1501 taaaatcgaa aacagacagc gacaacgttt gcggcttaac cccgatgatt attggccctg
     1561 ggatgtcagc ggaaagacga tagatgaaat cccgctcaaa ccgtttgagc aggtggcgga
     1621 tttgttggac aagaccattc gtgtgcaaaa actgatttgc ggcgcttttg cagacaactt
     1681 ggagcacatg agacaggctg gttttttcga tttggaaacg agacctcaca aggccccggg
     1741 ggggtacaac tatggtttgg aaatgacggg gatgcctttt atcttcatga acgccgcagg
     1801 catgcacacc gatgtgacga ctctcatgca tgaatccggg cacgcgatgc acactttttg
     1861 cacccataac gaaccgctga ttcagtaccg caattgcccg gccgaggtca gcgaggtggc
     1921 cagcatgagc atggaattga tttcgagtca tcattggcat gagttctatt cttcacaaga
     1981 tgtgcttcgc gcacggatag atcatttgga agacattgtg tccacgtttg cctgggtggc
     2041 tgtggttgat tcttttcagc actggctgta cacacacccg acgcacacgg tggcacagcg
     2101 ttctgcccag ttttctctca ttgcggctca gtatgaatca gggctggtgt cgtgggacgg
     2161 gtacgaatca ttcaaagaga atatgtggca aaaacagctc catattttcg aagtgccgtt
     2221 ctattatatc gagtacgcta ttgcgcaatt gggggctctg cagatatttg ccaattatca
     2281 gaaggatccc caaaggacta tcgaggccta tatcgcagga ttgcgtttgg gaagttcacg
     2341 gctcctgcag gaagtttgga aaaccctcaa tatcaaattt gatttttccg aggcgaacct
     2401 taaatcgctc atggccttcg tagagaggga gtggtccact ttgtgcgcag aatacgatgt
     2461 ggttaattaa cgcataaaat atgtgaggtt ttcattcgta gtccacgacc agggttgtga
     2521 ggagcacttg ttggtaggct cctgttcccc aagagacttg cacatcaact tttttggcgc
     2581 cagaattgat ttgtggtgtg accaaatccg aggaactcac ttcggtgatg gtgacgttct
     2641 tggtcagatt gtaactgcca ctcaggactt ctgcagggta attgtttgtg gtgacatagt
     2701 cataccctcg gaattgtctg tctgccaaaa tgatttcgat ctgctcgctg gccttttgcg
     2761 aggcaatgat gtggatttcc tgatcaactg aggtggcaac cacattcgac aatacactaa
     2821 gaccggtgac caagcctatg gccaggatcg tcatggccaa gacggcttcg agtagtgaga
     2881 atcctttaca attcagtttg ctgttcactt attttttctc cagaaacaaa ctaaccattg
     2941 gcactcagga catcgtcaag gagattttcg atctcttcaa ctttttcgtt gtgcaaagcc
     3001 acccctaaac cttttgcgtg ttcaaaatca ataaaagatc tgacggtagc cacaccaaaa
     3061 aaactcaccg aaaagagaaa acccaacaac atgagaatca tgatcgagta caaacaaaaa
     3121 ctgccctgat tgttacgcca ttgtttcact tttaagtccc tctaccacaa caaacaagca
     3181 atctctgtgc caacccttgt ggcctgataa ctcatgataa ttgtggttga aatgcccatg
     3241 ttttgggtat tggctggtga caggtatctg aagcatacat aattcggaat gattcctttg
     3301 aatcagtgca tgcggtggaa gggggactag ggtccaaaga ccaaagacca aagactcacc
     3361 cgtagatagc cattgaaaag ttttatttaa cttttttaac ccgggtggaa ctttgttggt
     3421 ggccggtcca gtagacgaag aaaagaaaaa tgttgtaaaa atacaaaatc cacaatgtgg
     3481 tgagcacaat ggaactcagg gagccgtaga caagattgta acgatcgagt tggcgcaggg
     3541 tgaaaaattt aaaaaggtat tttcccaact gcagggtcag cgaaaagaaa agggcacccc
     3601 acaacacgcg cttggggttg tttttttttg ccgcgagcgt ttgcatgatc acccagaaaa
     3661 gcacgcaccc caacagaaca aagccgccgc tggcaaagaa gttggcgtac tggtgccatt
     3721 gttcattcag cgaagtgatg cgcacggaga tcatttcgat gatgacgggg gtgaaaagaa
     3781 tgaacacgag acccagaagc cacgcaaacg aaatgaggtg tgtgaccaga aaatgccgtg
     3841 ttttcttggc ttgcaggatg tgattgatga tcctttcgaa attcacaaat agcatttgcg
     3901 ccgaaaacgc caggacaacc aagctgaacc acccaaaggt ttgtttcttt tcaatgaggg
     3961 cccacatgtt ttgcatgagc agatcggatt ggtagggcaa ggtctcatga atgagtttgg
     4021 tcaggctgtc ctggatgagg tcttgcgaaa tgaatgaacc ggcgatcgtg gccaataaca
     4081 agatggtcgg aaagatggcg atcaacgtat agaacgacag actggcagcc atgttcatga
     4141 tttccagccg acgatagtcg cgataatagc tcgagacgca agacgcgagg gatttcaaaa
     4201 aagagagaaa tgatttcatc ggaaaacagt ttttctggtt aaaagaaaac gaagtgtgca
     4261 cagaacacag agcccaacga caaaaagcca ggtggatcgt ggatcgtaga tcgtggatcg
     4321 taaagacaca gttgcctgtt gcttattgcc cgttgaaaat tgagacaatg aacatcctcc
     4381 tccctgtgct tgattgtagg tcgtgacgtt gatttgctca gaagcctgtt gatcgtctgt
     4441 agggtcattg ggattgaagc cgttatcgat ttcggttttg tcatccacgc caccgccatc
     4501 ggtgtcgggt ttgttggggt cggtctcgcc caatgtgggt tcgaaggcgc cattctgatt
     4561 tttatcctca tcaccatcca ggagcccgtc gccatcggtg tctgccgcgc tggggttcgt
     4621 ggggttggtt cccgtgagtt ctgtgcagtc atcaatcttg tcggcgtcgg tgtcgatctg
     4681 gttcggatcc gcctcgttta gaaaaggttc ataggcgccg ttgtggttgg cgtcctcaat
     4741 cccatccagg catgtatcgc cgtcggtgtc gggattgagg ggatcagtcg ggttatcgcc
     4801 gttgacttcg gtgccatcga gcagttcgtc atcatccgaa tccgcatcgt tgggatcggt
     4861 gagggtgttg tccatctcgt cccaatcctc gagcaaatcc tcgtcgtgat caaaaatggc
     4921 gtgaatctgt gattcattga tgtcgggaag gtaatcctcg ccgatcacca gggcatctgc
     4981 gaagatgagt aggtacacgt tgttgataat cgaggccatc cctgaggttt gaatgccttt
     5041 aaactctttg atttcgaatt cgaggtcgcg taccgaggtt gagggtgtgg cgtaaagtac
     5101 agtttgagcc aactccgtgc tgccgaaacc cgccgcgggg gtgagcgtgt caaacccaac
     5161 actcacatcg taaacaccca gaccgtccaa cgtggcggcc ggggccacac cgatgagtgc
     5221 gtcgacggtt gattcattaa aaccgctggt ggcgctatcg ccatcaagat caaccgcgat
     5281 ggcgatggtc tcgccggttc cgagatttgg ataatccact acaccaccat gacttgatga
     5341 cccgggatcg ccatcaccgt ccgtatcgcc aaagatcacc ttattgatcg tgctgatgcc
     5401 gatagacaaa ctgtcattgg ttccgtcgta gtagaaacat actttgtcga tgtcgaatcc
     5461 tgtgccggaa atgccaccga ccagaccgat gtcctgcccg cctgcatcga tcagacagtt
     5521 ttcattggta aagtccgcat ccacgtcgcc tgtcagggtg atggcggcgt aagctgggag
     5581 agaaatgatg acaagtgcca aaaaaacaca tttcgctgtg aaatttaaca tggtgcaacc
     5641 ctccggcaat gattgagtat cgttggggat catctagctt atctagttaa aaagtaaatg
     5701 aattaatgaa acaatgaatc aatcaagaaa tgaagaaatg tcccgcagag cgggatcaat
     5761 cctgctgagc cgtaggactc atcagacctt tggctgaggc tgaaagaaat gaaacaataa
     5821 tgaaaaaaat cataacagga tgagcgtgtg atctcccttt tcggtgactt gtccgatcac
     5881 acatgctttg gggaaagggg ccttaagctt gtgcaacaat gttggggcgt gtttttcgtc
     5941 cacgctgaga agcaaccctc cgctggtttg cgggtcgaaa aaaacgagct ggtcaagatc
     6001 agaaacggtg ttttcaatga tgaggtggtc tttcacgtat tgcctgttgg tgccatgggc
     6061 tttggtgagg cattctgtct tgagagtttc gtatacgtgt tcaaaccggg gcaaggcggc
     6121 tgaggtgatg gtcagggtga cgttgctggc cttggccagc tgataggcgt ggccggccag
     6181 tccaaaacca gtgacatcgg tcatggcgtg cagatattgc caatcttcac tttgcagaca
     6241 atcatgaata ttattaaggg tgcacatgga ggtggtggcc tcaatcaatt cactttctga
     6301 tttgacccct ttcttgaggg cagacatgag ggtgcctgtt cccaaaggtt tggtcaggat
     6361 gagtgtgtca ccgggtttgg caccggcgtt ggtgatgagt ttgttg
//
LOCUS       DATDUN010000605         2990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_32573, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000605 DATDUN010000000
VERSION     DATDUN010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32573"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="VJC18_08520"
     CDS             complement(<1..1351)
                     /locus_tag="VJC18_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45465.1"
                     /translation="MNKTTLNNHCFAFILLALITLLLTACGPDRFAPFNEIAPVNDAP
                     FTPTDDSATDANSESDPNTNNEVESVEPALDEGADSIPESNPDISVPLDSPEAIDVDE
                     NVIPDAPAENDPDDPSLDTTPDPAPDPDAGGLPADPVVDPDDADPVVAIEICDGFDNN
                     LNGQIDEGVSITVYADEDGDGYGDPETGVLVCGPTAGYVTNAEDCDDNNFATNPGIIK
                     DCSSISGLKKGLQFIDNDCDGVIDEDNACSPSPAGTLILPPDVTDDAGPTVTPLTPGT
                     TPININRGTMFFSGLHLEFTPNVDRGYRNFDVALQGIGEETSATQVTELTQTLITKDN
                     GGQTPFDSTLYEATVFYSDRTFKAYRGSITIDCDSNATNDGYCSKSKQFNLSNMEVFS
                     EWSTQDIRNGEYAFFVAVNGMSLEPTAGQGMEDLNVQAFIKNTTVHPKDGNASNQHYL
                     "
     gene            1509..1637
                     /locus_tag="VJC18_08525"
     CDS             1509..1637
                     /locus_tag="VJC18_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45466.1"
                     /translation="MRVFVQKEGHMKIIANNNTIQTSPATDQQTSPQSGHQLDVDE"
     gene            1651..1806
                     /locus_tag="VJC18_08530"
     CDS             1651..1806
                     /locus_tag="VJC18_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45467.1"
                     /translation="MPRSGAKYVELRGGAAGLLDTWLYRASARYFADPDRRQNDVGFR
                     CAWPVDK"
     gene            2121..>2990
                     /locus_tag="VJC18_08535"
     CDS             2121..>2990
                     /locus_tag="VJC18_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45468.1"
                     /translation="MSICLLAVLLLLALPAQTENVTEPDITYTAVSEQEGQIEEIAII
                     SDFNETDAARWNCVEGVGTTGQLEFGYVPPGMETTIELTPNLTVRLDFEVTEQAVSNG
                     TAFCRFERAIAPVESWQSATGIRIVLATPLSGVRRYHSLMLIDGSTKYIQTIEPTEYT
                     QPGQFLDRYLPFDQFKTTAGETLSAEALQDIKKFAVAIQTRDGDTLLIDKISVIKKIE
                     QTNWIRFATDHLALNLYEPGEPVILKFTKEHIVNPAIKGIEWEISDFNQAVVATGRVN
                     FTSGQTVYTVTQPP"
BASE COUNT          739 a          725 c          779 g          747 t
ORIGIN      
        1 agaggtaatg ttgattgctg gcattgccat ctttggggtg cacggtggtg ttcttgatga
       61 aggcctgcac gttcaggtct tccatgccct gaccggctgt gggttccaaa gacattccat
      121 tcaccgccac aaaaaaagcg tattcgccgt tgcggatgtc ctgtgtggac cactcagaga
      181 agacctccat attcgaaagg ttgaactgtt tgcttttgga gcaatagcca tcattcgtcg
      241 cattgctgtc gcaatcgatc gtgatactac cgcgatacgc cttgaaggtc ctgtcactat
      301 aaaacactgt tgcttcataa agcgtagaat caaaaggcgt ttgaccacca ttgtcttttg
      361 tgatcagggt ctgtgtcaat tcagtcacct gcgtcgcaga cgtttcttcc ccaattcctt
      421 gaagagcgac atcgaaattt cggtaacccc tgtcgacgtt gggtgtaaac tcaagatgca
      481 gtccactgaa aaacatggtg ccccgattaa tattgatcgg cgtggtgccc ggtgtcaacg
      541 gcgttactgt cggtcccgca tcatcagtca cgtctggtgg aagaatgagc gttcctgccg
      601 ggcttggact gcaagcatta tcctcatcta tcacaccgtc acaatcgttg tcgataaact
      661 gcaatccttt tttcagacca gaaatgctgg aacaatcctt gatgatgccc gggttggtcg
      721 caaaattgtt gtcgtcacag tcttctgcgt tggtgacgta tcctgccgta ggcccacaaa
      781 cgagcacccc tgtttcggga tcgccgtagc cgtctccgtc ttcatcggca tagaccgtga
      841 tggagacacc ctcgtcaatc tgaccattca gattgttatc gaaaccatcg caaatttcta
      901 tggcaaccac gggatcagcg tcgtcagggt ccactacggg atcagcaggc agaccacctg
      961 catcggggtc tggggctggg tctggcgtgg tgtctaaaga ggggtcatcg ggatcattct
     1021 ctgcgggcgc atcggggatg acattttcat cgacatcgat ggcctcgggg ctgtcgaggg
     1081 gtacagatat atctggattt gattcgggga tgctgtcggc accctcatcg agtgcaggtt
     1141 ccacgctctc aacctcgtta ttcgtgtttg ggtcagactc gctgttggca tcggttgcgc
     1201 tgtcatcagt gggtgtgaag ggtgcatcat tgacaggcgc gatttcgtta aatggggcaa
     1261 atctgtctgg cccacaggcg gtgagcaaaa gggtgatcag tgcaagcaga atgaatgcaa
     1321 agcagtggtt gtttagtgtt gtcttgttca tagtgttctc ccaaacggca cttttggcct
     1381 ctgtttcgta ttatcggata aaagtacgtg actgttgttt gggcgccaaa actaccgtgt
     1441 ccttgaggac acggagagtt tgtcgggtgg taactctcat gtgttgttct ccgataatcg
     1501 gggggaacat gcgcgtgttt gttcagaaag aggggcacat gaagatcatc gccaacaaca
     1561 acaccatcca aacgtcacca gcaactgacc agcaaacaag cccgcaatcg ggccatcaat
     1621 tggatgtcga cgagtgaccc cagcaacccc ttgcccagat cgggtgcgaa atacgtcgaa
     1681 cttcgtggtg gggccgcagg tttgctggat acgtggctgt accgcgcttc ggcacgatac
     1741 ttcgctgatc ccgacagacg tcaaaacgat gtgggttttc gttgcgcgtg gccggtagat
     1801 aagtgaagca aaagcagtgg tgatgtcgtc cctgaattcc actcccaatt agctggccaa
     1861 aaatatcacc tacggctcca acgaggcctt gttagaatag tctggtgaga ttgtttttta
     1921 agaatagaaa tttgcaacac gcttctcaga atgaaaatgg tcattcatga tttctcattg
     1981 tgaacaacga cacttgatgg cagataaaat aagtccaaca atcactcagc gataccgcaa
     2041 aaaaaaatcc tcaatccggc atggttattg cttcgaagtc agtgatgaag cgaacactga
     2101 cagctatccc aagatggtct ttgtctattt gcttgcttgc agtgttgttg ctgttggcct
     2161 tgccagcaca aacggaaaac gttaccgagc cagacatcac ctacactgca gtgagtgaac
     2221 aggaagggca gattgaggag atcgcgatca tcagcgattt taacgagaca gacgcggccc
     2281 gctggaactg cgtggaggga gttgggacca caggccagct ggaatttggt tatgtgccac
     2341 ctggcatgga aaccaccatt gaactgacac caaatctgac cgtacgactt gattttgagg
     2401 ttaccgaaca ggcggtcagt aatggaactg ccttttgccg attcgaaagg gccatcgccc
     2461 ccgttgaatc gtggcagtcg gcaactggga tcaggattgt cttggcaacc ccgctatcgg
     2521 gtgtcaggcg gtaccacagc ctcatgctga tagatggcag taccaagtat attcagacaa
     2581 tagaaccaac tgagtacaca cagcctgggc aatttcttga tcgctacctg cccttcgatc
     2641 agttcaaaac aaccgctggt gagaccttgt ctgccgaggc cctgcaagac ataaagaaat
     2701 tcgccgtggc tattcaaacc agagatggtg atacgctttt gatcgataaa atcagcgtga
     2761 tcaaaaaaat tgaacagact aattggatca gatttgccac cgatcatctg gcattaaatc
     2821 tctacgaacc gggtgaacct gtgatcctga agtttaccaa agaacatatt gttaatccgg
     2881 cgatcaaggg gattgaatgg gaaatcagcg atttcaatca ggccgttgtg gcaacgggca
     2941 gggtcaactt tacttcgggg caaaccgtgt acactgtgac acagccaccg
//
LOCUS       DATDUN010000606         1123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_610452, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000606 DATDUN010000000
VERSION     DATDUN010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610452"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VJC18_08540"
     CDS             complement(<1..435)
                     /locus_tag="VJC18_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLD45469.1"
                     /translation="MNIPLIDIKTINKTYVMGDTEVRALQGVSLTIERGEFVAIIGPS
                     GSGKSTLLHTLGFLDRPDSGQYYFDGYDVSKLNDDDLASLRNTNAGFVFQQFYLLSRM
                     TALENAELPLIYGGSSQVKETASQKLKDVGLADRANHKPNELS"
     gene            complement(514..>1123)
                     /locus_tag="VJC18_08545"
     CDS             complement(514..>1123)
                     /locus_tag="VJC18_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLD45470.1"
                     /translation="LATPVLAPIDGVVIVRSVEPGQTVVTGDAVLVLSDRLIVKAQID
                     EVDLGRIALGQTAQIRLDAYPQNVTAALVDHISFESTVVNNVTIYEVDVLPQEIPAVF
                     RSGMSATVDFFEKNNDHALLIPHSAVISQSGEDFVLVLNAQTAKQEQRKISLGLTDGE
                     NVEVTQGLTEGDVVLLQNKDGVLSQKKSTGKNPFMPNFEKKK"
BASE COUNT          307 a          288 c          230 g          298 t
ORIGIN      
        1 cgagagttca ttgggtttgt ggttcgcgcg gtctgccagc cccacatcct taagtttttg
       61 actggccgtt tcttttacct gtgaactgcc gccgtaaatg agaggcaatt cagcgttttc
      121 cagcgctgtc atgcgcgaca ataaataaaa ttgctgaaaa acgaaccctg catttgtgtt
      181 tcttaaactt gccaaatcat catcgttaag ttttgaaaca tcgtagccat caaaataata
      241 ttgaccggaa tcaggacgat ccaaaaaccc cagggtgtgt agaagtgttg attttcccga
      301 accagaagga ccaatgatcg ccacaaattc accgcgttca atggtcaggg aaacaccttg
      361 caaggcacgc acctccgtat cgcccatgac ataggtcttg ttgatggttt tgatgtctat
      421 aagagggatg ttcattgcaa catcatttca aagtccgtca ttgcgagtcg acctgagttt
      481 agcgaaggac gacgtggcaa tccaaatatt gattcatttt ttcttttcaa aattaggcat
      541 gaagggattt ttgccagtag actttttttg gctcaacaca ccatctttgt tttgcaaaag
      601 caccacatcg ccctcagtca gcccctgagt cacttcaaca ttttccccat ctgtaagacc
      661 taaactgatt tttcgttgtt cctgttttgc tgtttgggca ttcaatacca atacaaaatc
      721 ctcgccactt tgtgaaatca ctgcggaatg aggaatcaac agggcgtggt cattgttttt
      781 ttcaaaaaaa tctaccgtgg cgctcatgcc cgaacgaaac acagctggaa tctcctgtgg
      841 caaaacatcc acctcataaa tcgtcacatt attcaccact gtggattcaa aagagatgtg
      901 atccaccagc gcagcggtca cattctgagg gtaggcatca agacgtattt gagccgtctg
      961 ccccaaagca atgcgtccca agtccacttc atcgatctgc gccttcacaa tcaaacgatc
     1021 cgacaacacc agaacagcat cacccgtcac cacagtttga ccgggctcta cagagcgaac
     1081 gatcaccaca ccatcaatgg gcgccaatac cggtgtggcc aga
//
LOCUS       DATDUN010000607         1018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_487611, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000607 DATDUN010000000
VERSION     DATDUN010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487611"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VJC18_08550"
     CDS             complement(<1..978)
                     /locus_tag="VJC18_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45471.1"
                     /translation="MENKQLSRLIRIGNFDESGWRNEACQVVMRLVHPSAGQGSTVTL
                     ELPHLRQNEIKGVSCNRVEVRCENELPDEELLFNIDGQSLPLLDSISEVQRQIVDLEG
                     RKISVKGIRFTNMYGLDDPRYLHGGMGIDFKVTKKDAPHIGDVARFLGARLFGTDQFV
                     AHGQGFELALDGVRVLISVNQELGRGHISFEPTQAFENGATLLTLDAALLNLMRYPKP
                     ASSFTPQENPQRDQFIEFNSQTPFTLDDLLEKHAEAVPPATAPTLRVREEAMKAFTGQ
                     LGSGVPANQQHRRFHLLLRGKYPVLLTINAGNDVARWSIRLDMSRSFKNG"
BASE COUNT          274 a          284 c          226 g          234 t
ORIGIN      
        1 gccattttta aaagagcgtg acatatccaa gcgaatggac caacgggcca catcgttacc
       61 cgcattaata gtcaatagca cggggtactt gccgcgcagg agcaaatgaa accgccgatg
      121 ttgttggttt gcaggcacgc cagatcccag ctggcctgtg aaagccttca tggcctcctc
      181 ccgcaccctc aaggtggggg cagtggctgg cggtacggcc tctgcgtgtt tttccaatag
      241 atcatcaagg gtaaatggcg tttgagaatt gaactcaata aattgatccc tctgagggtt
      301 ttcttgaggg gtaaacgaag aagctggttt aggatacctc ataagattca aaagggccgc
      361 gtcgagtgtt agcaaggtcg cgccgttttc aaaggcctgt gtgggttcaa aagatatgtg
      421 cccccgacct aactcttgat ttaccgaaat cagcacccgc acaccgtcta gcgccaactc
      481 aaacccttga ccatgagcaa caaactggtc tgtgccaaac aacctcgccc ccaaaaatcg
      541 cgcgacgtca ccgatgtgag gcgcatcttt ttttgtgacc ttaaaatcaa tccccatacc
      601 accgtgcagg tatctgggat catcaagacc atacatattg gtaaagcgaa tccctttgac
      661 actgatcttt ctgccctcta gatcaacaat ttggcgctga acctcgctaa tggaatccaa
      721 gagaggcaac gactgaccat cgatgttaaa aagaagctct tcatcgggta attcattctc
      781 acatcgcacc tcaacccgat tacacgaaac cccttttatt tcgttctgtc tcaagtgcgg
      841 aagttcaagc gtcactgttg acccctgccc cgcactggga tgcaccagcc tcatgacaac
      901 ctgacaggct tcattccgcc agccagactc gtcaaagttc ccaatgcgaa tcaaacgaga
      961 caattgcttg ttctccaaat cgaaaagcgt cggccaaatg gcaaaacgaa accccacc
//
LOCUS       DATDUN010000608         4465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_152403, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000608 DATDUN010000000
VERSION     DATDUN010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152403"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VJC18_08555"
     CDS             <1..376
                     /locus_tag="VJC18_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HLD45472.1"
                     /translation="LEWAAFLDKLYNRDFDVVTLACTMPFEADPYQVWDMSQANIKSS
                     SNYISYVNVEASKLMREAREEFDEEKRNVKYRLFHKIIHEEQPYTFLYNQPNIIAVSK
                     RFGNVKTHPAGLELREWTVRNR"
     gene            393..1550
                     /locus_tag="VJC18_08560"
     CDS             393..1550
                     /locus_tag="VJC18_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLD45473.1"
                     /translation="MNRERAVLRSTNHDPRSTIMFFYIFKRLLLMIPTLIGITFMTFL
                     IMKLAPGDPVKLKLMFVEEGVDADALAAVLKSETPAIQLSEEYLEFARKISDAVSGKK
                     KALADYEQDRSYKTAKYVGENTVFYLKWLRNIAVLDFGRSFKDRQPVIEKIKSALPIT
                     LLINILAILIVYSVSVPLGIWSAIKQGSVIDKMVMVKLFVLYSLPTFWVATMLLMFLA
                     GGEYLNWFPIVGYKSDYFNQLGLLGKMFDVAWHLVLPIAASTIGGFAFLTRFSRSNFL
                     DVISQDYIRTARAKGLPESKVLFKHGLRNALIPFVTLMGTLLPALLGGSVIIEQIFSI
                     PGMGMLSFEAVLGRDHNLIMGTATIGAFLTLLSLLLADLMYVVVDPRIRLE"
     gene            1661..2677
                     /locus_tag="VJC18_08565"
     CDS             1661..2677
                     /locus_tag="VJC18_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLD45474.1"
                     /translation="MKKQKRKTFSRLIWDKFWGAYLNRVGFVFVLLAVLLALAAPFLA
                     NNKPYYYQHEGKTTFPVLEDVFLVGRFVTYPQFLETDFRKLQNKSAHSFVMPPIPYSP
                     YEFDLDSILLPPSKDHLMGTDDQGRDVAARMLYGAQISLSVGFVAVGIYVLIGIIIGS
                     LAGYFGGWPDIIISRFIEIVMCFPTFFLILAIIAFIEPSLYKIMIVIGFTGWTGIARL
                     VRGEFMKLRGQDFVTSARALGVSTTGIIFRHILPNALSPVLVSITFGVASSILVESSL
                     SFLGFGVQPPTASWGQILSQSREYMDIAWWLMAFPGAAIFLTITAFNLMGEGLRDAVD
                     PRGG"
     gene            2902..3876
                     /locus_tag="VJC18_08570"
     CDS             2902..3876
                     /locus_tag="VJC18_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLD45475.1"
                     /translation="MNILEIKNLRTSFTTRQGKIYAVDDVSLDLPKGKITALVGESGS
                     GKSVTAMSVLRLIQAPGRVEGGRVLLHTQDGERDVLALAEEQVRQTVRGTRIAMIFQE
                     PMTSLNPVYTVGDQIMEAIALHQKVSGIQARALAVDLLERVGIPNPESRIDNYPHEMS
                     GGMRQRVMIAMGLSCEPELLIADEPTTALDVTIQAQILELIKKLVDETGMSVLLITHD
                     LGIVAAYATKVAVMYAGQIVEEADTMSLFKSPKHPYTKGLLASIPSLQGKAEKRLHAI
                     PGMVPSLLDLPIGCRFQARCERKAPECGLGPIGLTQEGERQWRCVNTY"
     gene            4159..>4465
                     /locus_tag="VJC18_08575"
     CDS             4159..>4465
                     /locus_tag="VJC18_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLD45476.1"
                     /translation="MRQGLFGGRRQSVKAVTDVSLELKQGEVLGLVGESGCGKTTLGR
                     LILRLIKPDRGDILFNGVNITSFSNAQMRPLRKDMQVIFQDPFSSLNPRMCIGKAIME
                     "
BASE COUNT         1032 a         1027 c         1247 g         1159 t
ORIGIN      
        1 cctggaatgg gcggccttcc tcgataagct ctataaccgc gattttgacg tggtgacact
       61 ggcgtgcaca atgccgtttg aggccgatcc gtatcaggtg tgggatatgt cgcaggccaa
      121 catcaaaagc agttcaaact acatttccta cgtgaacgtg gaggcctcca agctcatgcg
      181 cgaggcgcgc gaagaatttg atgaagaaaa gcggaatgtc aaatatcggc tgtttcacaa
      241 aattattcac gaggaacagc cgtatacgtt tttgtataac cagcccaaca tcattgccgt
      301 gtcaaagcgg tttggcaacg tgaagaccca ccccgcgggg ttggagctga gggagtggac
      361 ggtgagaaac cgttgatcgt ggatcgtgta tagtgaatcg tgaaagagct gttttgcgat
      421 ccacgaacca cgatccacga tccactatta tgtttttcta tatattcaaa cgcctcctcc
      481 tcatgattcc cacactcatc gggatcacgt tcatgacgtt cctcatcatg aagctcgctc
      541 ccggtgatcc ggtcaaactc aagctcatgt tcgtggaaga gggtgtggat gccgacgcgc
      601 tggccgctgt cctcaagagc gaaacccccg ccatccagtt gtccgaagag tatctggaat
      661 ttgcccgcaa aattagcgat gctgtttcgg gtaaaaagaa ggccttggcg gactatgagc
      721 aagacaggtc ttacaagaca gccaaatatg ttggtgagaa caccgtgttt tatctcaagt
      781 ggctcagaaa catcgctgtg ctcgattttg gccggtcgtt caaggatcga caaccagtca
      841 tcgaaaaaat caagagcgcc ttgccgatca ccttgctcat caatatcctc gcgatcctca
      901 ttgtgtactc agtctccgtt cctttgggga tttggtcggc gattaaacag gggagcgtga
      961 tcgacaaaat ggtgatggtc aaattgtttg tgttgtattc tctgcccacg ttctgggtgg
     1021 cgaccatgtt gctcatgttt ctggcggggg gtgaatacct gaactggttt cccatcgttg
     1081 gatacaaatc cgattatttt aatcagcttg gtttattggg aaaaatgttc gatgtggcct
     1141 ggcatttggt gttgcctatc gccgcctcaa ccattggtgg ctttgctttt ttgacccgtt
     1201 tttcgcgcag taattttttg gacgtgatca gtcaagacta catcagaacc gccagggcca
     1261 aggggttgcc tgaatccaag gtgttgttca aacacggcct gcgcaatgcc ctcattccct
     1321 ttgtgacctt gatggggacc ttgttgcccg ctctgctggg cgggtctgta atcattgaac
     1381 aaatattttc aatccctggc atggggatgt tgtcgttcga ggccgtgtta ggtcgcgatc
     1441 ataacctgat catgggcacg gccacgattg gtgctttttt gaccctgctc agcctgctgt
     1501 tggcggatct gatgtatgtg gttgtggatc cgaggatcag gctggagtga ggggaagaac
     1561 gaaaggacga gggacccttc gactgcgctc agggcaggcg aggggcgaat aaacaaatga
     1621 atgaatgaat gaatcaatca agaaatgaaa caatgaacta atgaaaaaac agaaacgcaa
     1681 aacattctca cgtttgatct gggataaatt ttggggcgcc tacctcaatc gtgtggggtt
     1741 tgtgtttgtt ctgcttgctg tcttgttggc cctggcggca ccgtttttgg ccaacaacaa
     1801 gccctattac tatcaacacg aagggaaaac aacgtttcct gttttggagg atgtgtttct
     1861 cgtcggccgc tttgtgacct acccccagtt tttagaaact gattttcgca aattacaaaa
     1921 caaatcagct cactcgtttg tgatgccccc tattccgtat tcgccgtacg agttcgattt
     1981 ggacagcatt cttctgcctc cttcgaagga tcacttgatg ggcacggatg atcaaggccg
     2041 cgatgtggcg gcccgcatgc tctacggggc ccaaatcagt ctgtcggttg gttttgtggc
     2101 agtgggaatc tatgtgctca tcgggatcat catcggttct ctggccggtt attttggcgg
     2161 ctggcccgac atcatcattt cgcggtttat tgaaatcgtg atgtgctttc ccaccttctt
     2221 tttgatcctg gcgatcatcg cgttcattga gcccagtctg tacaaaatca tgatcgttat
     2281 tggctttacg ggctggacgg gcattgcccg tctggtgagg ggcgagttca tgaaacttcg
     2341 tgggcaggat tttgtgacga gtgcccgggc cctgggggtg tcgacgacag gaatcatttt
     2401 cagacacatt ttgcccaacg cgttgtcgcc ggttttggtg tcgatcacgt ttggtgtggc
     2461 gtcatcgatt ttggtggaat cctctctttc ctttttgggt tttggggtac agccgcccac
     2521 ggcgagttgg ggacagattt taagtcagtc gcgcgaatat atggacatcg cctggtggct
     2581 catggccttt ccgggcgcgg ccattttttt aacgatcaca gcgtttaatt tgatgggaga
     2641 gggcctgagg gacgcggtgg atccacgggg gggataaacg agcttacgag cgcacgagct
     2701 tacgagggac ccttcgactg cgctcagggc aggcgaggga cgaaacaatt gagcaatttg
     2761 caatgggcaa tcgtagcaat tgcggcaatt gcaatgattg caacaattgc agtaattgct
     2821 acagttgatg acgataagta ccctcaaggg cacaagcgaa accttctacg cgatacgctt
     2881 ttcctttgag ctgtttttaa catgaatatt ttagaaatca aaaacctccg aacatccttt
     2941 accacccgtc agggcaagat ttatgcggtc gatgatgtga gcctggattt gccaaagggc
     3001 aaaatcactg cactggtcgg cgaatccgga agcggtaagt ccgtgacggc catgagtgtg
     3061 ttgcgtttga ttcaggcgcc cggcagggtt gaggggggca gggttttgtt gcacacacag
     3121 gatggtgagc gggatgtgtt ggctctggcg gaagaacagg tgaggcaaac agtgcgaggc
     3181 acccgcattg ccatgatttt tcaggagccc atgaccagtc tcaatcccgt gtacacggtg
     3241 ggagaccaga tcatggaggc cattgccctg caccagaagg tgagcgggat tcaggctcgg
     3301 gcactggctg tcgatctgct ggagcgtgtg ggcattccca accccgaatc gcggatcgat
     3361 aattacccgc acgaaatgag tggcggcatg cgacagcggg tgatgatcgc catggggctt
     3421 tcatgcgagc ccgaattgtt gatcgccgat gagcccacta cggcgctcga cgtgaccatt
     3481 caggcgcaga tattggaact catcaagaaa ttggtcgacg aaacgggcat gtcggttttg
     3541 ctcatcacgc atgatttggg gattgtggcc gcgtatgcca ccaaggtggc cgtgatgtac
     3601 gcaggacaaa tcgtagagga agccgacacg atgtcgttgt tcaaaagccc caaacaccca
     3661 tataccaaag gtttgctggc atcgatcccc agtcttcagg gcaaggctga aaagaggttg
     3721 cacgcgattc ccggtatggt gcccagtttg ttggatttgc cgatagggtg tcggtttcag
     3781 gctcggtgtg aacgcaaagc accggaatgt gggctcggtc ccattggctt aacacaagag
     3841 ggggagaggc aatggcggtg tgtgaatacg tattaatttt tggctcaagg ccaaaggctt
     3901 aaggcgaaag gcatatggta ctgaatatcg aatatcgaac aaggaatgtc gaatatcgaa
     3961 atttgtcatc cctgcgaagg cagggatcca gaattaatgt aaacatggat tcctgcttgc
     4021 gcaggaatga caatcacttc gaaaatcctt gctcattatt cgacattcgt tttcttcctt
     4081 tgagctttga gctttgagct ttcagctgtt tttgacatga atattttaga aataaaaaat
     4141 cttcataaaa cgtttccggt gcgccagggc ctgtttgggg gacggcggca gagtgtcaag
     4201 gcggtaactg atgtcagtct ggagctaaaa cagggcgaag tcctggggct tgtgggggaa
     4261 tcgggttgtg gcaagaccac actgggacgg ctcatcttga gactcatcaa gcccgatcgg
     4321 ggtgatatcc tgttcaacgg ggtgaacatc acatcgtttt ctaatgcgca gatgaggccc
     4381 ttgcgcaaag acatgcaggt gatttttcag gacccttttt cgtctctcaa cccgcgaatg
     4441 tgtattggca aggccatcat ggagc
//
LOCUS       DATDUN010000609         2630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_487763, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000609 DATDUN010000000
VERSION     DATDUN010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487763"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VJC18_08580"
     CDS             complement(<1..973)
                     /locus_tag="VJC18_08580"
                     /inference="COORDINATES: protein motif:HMM:NF026052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45477.1"
                     /translation="MIKFDESFYRRSVIPGGQAVVERSYPVGRKNFFRQSKTSLLSVR
                     VCFDEQVTAPDKVCLHFENFAHNKSRVYTERLQGHSREFTCRVFPRFHGVTRFKIKYR
                     LRRRWYWDNRPFSYFIVDPHRLRDIRMYTLIPNVSGHVGDWSQDLERIQALGFNTVHI
                     LPVTQMGISESPYAARDLFNLDTAFVNPSDAMPAEQQIESFVERACSLNMRLCFDLVF
                     NNIGVDSLICRMKPEWLTEDPDEEDGIRRGGWDDGRQWHKWQDLAFINYDIPCSYTRD
                     ALWDHMRAYALYWSGLAARTNGMIRLDNLHSSHPLFIRWVLGEIRKAF"
     gene            complement(973..>2630)
                     /gene="glgP"
                     /locus_tag="VJC18_08585"
     CDS             complement(973..>2630)
                     /gene="glgP"
                     /locus_tag="VJC18_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HLD45478.1"
                     /translation="AKSLVWTTSVFTTHTPVPAGNETFSRSLIEKYFTSYIGEIGITW
                     EKFMSLSSIDPKNDKENFSMTILALKLSAYANGVSRLHGEVSRKMWRDLYAGVPSHEI
                     PIGAVVNGVHSRTWLSPHLYNLFLRYGGPSLINELADFSIWQTVRRIPDEELWAVREQ
                     DRRRMVEYMRKKFKLQLKSKGLHATDYGKIEDLLDPKALTIGFARRFATYKRGHLIFK
                     DLERLKKIFSNEKYKVQMIIAGKAHPADQAGKDIIKQIYEIANKSDFRGKIVFIENYD
                     LDIASYLVQGVDIWLNNPIRPMEACGTSGMKAAINGGLNLSVLDGWWDEAYTQDVGWA
                     IGEREEYDDQKNQDRIESDLIYGILEKDIIPLFYKRDALGVPKEWVGMMKNAIAELGT
                     GFNSHRMLRDYVENYYVPAEKSYVRLSQNDCAEAKELAQWRTDIISHWSDIEIAKVES
                     ITDDFIYKGSQLDVKASVKLGAIKAENILVECYYGSLDNQDKFEETKTIRMQMVGQEN
                     GFSVFQAAIPCERGGRFGYTVRVLPGHPQLTINTLPGFIKWHE"
BASE COUNT          638 a          740 c          615 g          637 t
ORIGIN      
        1 gaaatgcttt gcggatctcc cccagcaccc aacgaataaa cagggggtga ctcgagtgaa
       61 gattatcgag tctgatcatc ccattggttc tcgccgccag accagaccag tacagagcgt
      121 aagcccgcat gtgatcccac agggcatcgc gtgtgtaaga gcagggaatg tcgtaattga
      181 tgaacgccaa atcctgccat ttatgccact gcctcccatc atcccaaccg cccctccgga
      241 taccatcttc ttcgtcggga tcttctgtca gccattcagg cttcatgcga caaatgaggc
      301 tgtcgacccc aatgttgttg aagacgagat caaaacacaa acgcatattc aaactgcacg
      361 cgcgttcaac aaagctctcg atctgttgct ctgccggcat ggcatccgaa gggttgacaa
      421 aggcagtatc cagattgaac aaatcacgcg ccgcgtaagg gctctccgag atgcccattt
      481 gtgtcacggg aagaatgtgc acggtgttga aacccagcgc ctgaatgcgt tccaggtcct
      541 gactccaatc ccccacgtga ccactcacat tgggaatcag cgtgtacatt ctgatatccc
      601 gcaagcgatg agggtcaacg atgaaatagg aaaagggcct attgtcccaa taccatctgc
      661 gtcgcagtcg gtatttgatt ttgaaacgcg tcaccccatg aaacctggga aacacgcgac
      721 acgtgaactc cctgctgtga ccttgcagac gctcggtgta cacccgcgat ttattgtggg
      781 cgaaattctc aaaatgaaga caaactttgt cgggggctgt gacttgctca tcgaaacaga
      841 cacgaacgct caacaggctg gtttttgatt gccgaaaaaa attcttcctg ccaacgggat
      901 acgaacgctc caccacggcc tgcccgccgg gaataacact gcgcctgtaa aatgattcgt
      961 cgaattttat catcattcat gccacttgat gaaaccgggc aacgtattga tagtcaactg
     1021 tgggtgtccg ggaagcacac gtaccgtgta accaaaccga cccccacgtt cgcagggaat
     1081 ggccgcctga aataccgaaa acccgttctc ctgcccaacc atctgcatcc tgatggtctt
     1141 ggtctcctca aatttatcct gattgtccag cgacccatag taacattcca ccagaatatt
     1201 ttcggccttg atcgcgccca gcttgacact ggccttcaca tcgagctgac tacccttgta
     1261 gatgaaatca tcggtgatgg attcgacctt ggcaatctcg atatcgctcc aatgtgagat
     1321 gatgtctgtg cgccattgag caagttcctt ggcctcggca caatcgttct gtgaaagccg
     1381 cacataggat ttttccgcgg gcacataata attttcgacg taatccctga gcatcctgtg
     1441 cgaattaaat ccagtcccca attcagcaat ggcgttcttc atcatcccca cccattcctt
     1501 ggggacaccc agcgcatctc gtttgtagaa caggggaatg atgtcttttt cgaggatccc
     1561 gtagatcaga tcgctctcga tacgatcctg atttttctga tcgtcgtatt cctcgcgctc
     1621 gccgatcgcc caacccacat cctgcgtgta ggcttcgtcc caccagccat ccagtacaga
     1681 caggttcaaa ccgccattga tggcggcctt cataccggaa gtcccgcaag cctccatggg
     1741 tcggatgggg ttgttcaacc agatatcgac gccctgcacc aggtagctgg cgatatcgag
     1801 atcgtaattc tcgataaaaa caatcttgcc tctgaaatcg gatttgttgg cgatttcgta
     1861 aatctgtttg atgatgtcct tgcccgcctg atcagcggga tgagccttgc cggcaatgat
     1921 catctgaacc ttgtattttt cgttggaaaa gatttttttg agtcgctcca gatccttgaa
     1981 gatgaggtga ccccgcttgt acgtggcaaa gcgccgcgcg aacccaatcg tcagggcttt
     2041 gggatccaac aagtcctcga tcttgccata gtcagtcgcg tgcaggccct tgctcttgag
     2101 ttgcagtttg aattttttgc gcatgtattc aaccatcctg cgccgatctt gctcgcgcac
     2161 cgcccacaat tcttcgtcgg ggatacgacg cacggtctgc cagatactga aatctgccaa
     2221 ttcgttgatc aggctgggac caccgtaacg caaaaacaga ttgtacagat gaggagacaa
     2281 ccaggtacgc gaatgcaccc cgttcacaac agccccgatc ggaatttcgt ggctgggcac
     2341 cccggcatac aaatccctcc acattttgcg agacacctca ccatgcagtc tggaaacgcc
     2401 attggcatac gcggataact tgagcgccag aatggtcatg ctaaaatttt ccttgtcatt
     2461 tttgggatca atcgagctca aggacatgaa cttctcccag gtgattccga tctccccgat
     2521 gtaactcgta aaatatttct cgatcagact gcggctgaag gtctcattgc cggccggaac
     2581 gggtgtgtgc gtggtaaaca ccgaagtagt ccacaccaga ctcttggcct
//
LOCUS       DATDUN010000610         1468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_111131, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000610 DATDUN010000000
VERSION     DATDUN010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111131"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="glnS"
                     /locus_tag="VJC18_08590"
     CDS             complement(<1..999)
                     /gene="glnS"
                     /locus_tag="VJC18_08590"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HLD45479.1"
                     /translation="MNTNKETNAPPTIPSNFIRNIIDDDLASGKHAKIVTRFPPEPNG
                     YLHIGHAKSIHINFGLARDYNGVCHLRFDDTNPSKEEDEYVQSIQNDVRWLGFDWKDK
                     LFFASDYFDQLYEWAVYLISQGKAYVCDLSPEEISQYRGTSGEQIECGKNSPYRDRSV
                     EENLSLFDRMKQGEFKEGEKCLRAKIDMTSPNINMRDPVLYRIMRQSHHRTGDKWCVY
                     PMYDFAHGQSDAIEGITHSICTLEFEIHRPLYDWFVQNLPVPHKPRQIEFARLNLNYT
                     VMSKRHLLKLVQNKAVNGWDDPRMPTLAGVRRRGYTPDALKDFCERIGVAKADSVVD"
     gene            1156..>1468
                     /locus_tag="VJC18_08595"
     CDS             1156..>1468
                     /locus_tag="VJC18_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45480.1"
                     /translation="MGRDLKNIFESLVKIDNILVWDGTKRPFFESYYLNATSQDAKWG
                     TLLRYTLSTSTAKQVGDTATVLCLFVDSQGNKVVAKESFDLLKHDIVHSDRFISLGSS
                     YL"
BASE COUNT          356 a          369 c          339 g          404 t
ORIGIN      
        1 atccacaaca gaatccgcct tggccacgcc aatgcgttca caaaaatcct tgagggcatc
       61 tggagtatag cctcgtctgc gaacaccggc cagcgtgggc atgcggggat catcccagcc
      121 gttcactgct ttgttttgca cgagcttcaa tagatgccgt ttgctcatga ctgtgtaatt
      181 caaattcagg cgggcaaact cgatctgacg cggcttgtgc ggcacaggta aattctggac
      241 aaaccagtcg tacaagggcc ggtggatttc aaactcaagt gtgcaaatgg aatgggtgat
      301 tccctcgatc gcatcggatt gcccatgcgc aaaatcgtac atgggataaa cacaccactt
      361 atctcccgta cggtgatgcg actggcgcat gatccggtac aacacagggt cacgcatgtt
      421 aatgttaggc gacgtcatat caatcttggc gcgcaaacat ttttcgccct ctttgaactc
      481 gccttgtttc atgcggtcaa aaagcgacaa attctcttcc acactacggt cacgatacgg
      541 actgtttttg ccacactcaa tctgttctcc gctggttcct cgatactggc tgatttcctc
      601 ggggctcagg tcgcacacat acgccttgcc ctggctgatg agatagaccg cccattcata
      661 aagctggtca aaataatcgg aggcaaaaaa gagtttgtct ttccagtcaa aaccgagcca
      721 cctcacatca ttttgaatgg actgcacata ctcatcttct tctttgctgg ggttggtgtc
      781 gtcgaatctt aaatgacaaa caccgttgta gtcacgcgcc aagccaaaat tgatgtgaat
      841 cgatttggcg tgtccaatgt gcaaatagcc attcggttct ggtggaaacc gtgtgacaat
      901 ctttgcgtgt ttgccgctgg ccagatcatc gtcaatgatg ttgcggatga aatttgaagg
      961 gatagttggt ggtgcgttcg tttctttgtt tgtgttcata atatggctct tcttgctccc
     1021 gcctgatttt aggcctcagg acagggggga ttgactgctt tatattatcg gcccggtccg
     1081 aacacaactg ggtttttagg tttcactttg ttccaaaggc ttgacacaaa ccgtcgagtc
     1141 ggtacaagag caactatggg tcgagaccta aaaaacattt ttgagtcttt ggttaaaata
     1201 gataatattt tggtctggga tggaacgaaa cgcccttttt ttgaatccta ttaccttaac
     1261 gccacttcgc aagacgcaaa gtggggcaca ttgctcagat acacgctatc cacatcaacc
     1321 gccaaacagg ttggagacac ggcaaccgtc ctctgcctct ttgtcgattc tcagggcaat
     1381 aaagtcgtcg ccaaagaatc ctttgacctc ttaaagcacg acattgttca ttcagaccgg
     1441 ttcatttccc tcggctcttc ctaccttt
//
LOCUS       DATDUN010000611         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_111132, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000611 DATDUN010000000
VERSION     DATDUN010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111132"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..907)
                     /locus_tag="VJC18_08600"
     CDS             complement(92..907)
                     /locus_tag="VJC18_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HLD45481.1"
                     /translation="MIEEFYKPTTVQEALEQKKKFGDQALYLGGGVEVNNLFFPNKPK
                     HLISLEGLQLNQIVADQKHLVLGSGCTIQQILESDLVPDVLKKACLNLTNRNIRNQAT
                     IGGHIATNKSCSDLLPTLIVLGTELEITESAQTKTVLLEDYINSEGRDLITSVMIPQL
                     SEQTKVSILNFTRSANDVSILTAAVSYVLEHDVLKNVKIAVGGVAKHVIRLSTVEKQL
                     AEKTMPCREDVEAMVKEVVCPITDIRGTAEFKKYEIAVMVGGCFISSKLKAER"
     gene            complement(1160..>2113)
                     /locus_tag="VJC18_08605"
     CDS             complement(1160..>2113)
                     /locus_tag="VJC18_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HLD45482.1"
                     /translation="NPVSAQPAVQKTEPVVDRGIQSPQADATEVSTQMLSPITAQSAR
                     LPLGPSGFGRTLPSVMDSAEIFVARNMVKYAGRMEILVNATGQPIIKFTDEKTGQTTF
                     FKPSEILSVRERRVGLDDPKHGLCRVASPQLKDKSLNVSQSGKQVLVDLADPRLQSFL
                     HSRFGALQLGLQRGTLDKREAAIRAYEIIRHQVRYDESRLVQGGGLAVRMYSLSEFLD
                     QGVCNERGMLLQVALQYIGVESRFEKGTVLPSSSGGKEGRHAWVRVTDPAVTSDVLIL
                     DPQQAKPQTEYRKGDSRLIPDPDPAYLRDGSQTVALRPGKG"
BASE COUNT          499 a          573 c          472 g          569 t
ORIGIN      
        1 aaagatcaac ctgtcattcc tgcgaaagca ggaatccaag attctttcaa ttctggatcc
       61 ctgctttcgc agggatgaca atcttttcct ttcacctttc agctttgagc tttgagctaa
      121 taaaacaccc ccccaccatc accgcaatct catatttttt aaactccgcc gtccccctga
      181 tatcagttat cgggcacacc acttctttaa ccatagcctc aacatcttcg cggcaaggca
      241 tggttttttc agccaattgt ttttcgactg tagagagtct gatcacatgt ttggccacac
      301 cacccactgc gatttttaca ttttttaaaa cgtcatgttc caacacatag gaaacggccg
      361 ctgttaatat ggaaacatcg ttggccgagc gcgtaaaatt gaggatcgaa accttggtct
      421 gctccgataa ttgagggatc atgacggagg taatcaggtc acggccctcg ctattgatat
      481 aatcctcaag caaaacggtt tttgtttgtg cggactcagt aatttccaac tccgttccca
      541 acacgatcag tgtgggcaaa agatcactgc aggatttatt ggtggcgatg tgtccaccga
      601 tcgtggcctg attccgaata ttgcgattgg tgagattcaa acacgccttt ttcaggacat
      661 cgggaaccag atcactctca aggatttgct ggatggtgca acccgatccc aacacaaggt
      721 gcttttgatc agcaacgatt tgattgagct gtaacccttc cagactgatg aggtgttttg
      781 gtttgttagg aaaaaaaaga ttgttcacct ccacaccacc gcccaaataa agggcctgat
      841 ctccaaactt ctttttttgc tccagagctt cctgaacagt cgttggttta taaaattcct
      901 caatcatccc ccctccttat tcgagaaatt atcgtcaagt catatatttt ttgtcattcc
      961 tgcgaaggca gggaccacgc agggatgaca acatcccagt aagtcacctg aatcgacaac
     1021 ctctcgaggg agaatatagc caaacgtttg gaaatgaggc aagctttctt tgaaaccagg
     1081 ttgcacatca taaatttcgt cagttaatga gccctatcgc tctttccttt cgacattcga
     1141 tattccttgt tcgctattct tatcccttgc ctggccgcag ggccacagtc tgtgaaccgt
     1201 ctcggagata ggcgggatcg gggtcaggaa tcaggcggct gtcacccttt cggtactccg
     1261 tttgtggttt ggcctgttgc gggtccaata tcaaaacatc actcgtcacg gccggatcgg
     1321 tcacacgtac ccaggcatgc cgaccttcct ttccgccact ggaggagggc aacacggttc
     1381 ctttttcgaa acgggattca accccgatgt attgcagggc cacctgcagg agcatgcccc
     1441 gttcgttgca gaccccctga tccaaaaact cactcaaact gtacatccgc acagccaggc
     1501 caccgccctg taccagacgc gactcatcat aacgcacctg atggcgaatg atctcgtagg
     1561 ccctgatggc cgcctctctt ttatcgagtg tgcctctttg taaacctaac tgtagagcac
     1621 caaatctgct gtgtaaaaaa gactgcaatc tgggatcggc caaatcgacc aaaacctgtt
     1681 tgccgctttg cgacacattg agtgacttgt ctttaagttg cggcgaggca acgcgacaaa
     1741 gcccgtgttt cggatcgtcc agacccaccc gtctctctct gacgctcaga atttcgctgg
     1801 gtttgaaaaa ggtagtctgc cctgttttct cgtcggtaaa tttgatgatc ggttggcctg
     1861 tggcgttgac cagaatctcc atgcgcccag cgtattttac catgttcctg gcgacaaaga
     1921 tttctgcgct atccatgaca gacggcaacg tccgtccaaa tcccgacggc cccaagggaa
     1981 gccttgcgct ttgtgccgtg atgggactca acatctgcgt actcacttcg gtggcatcgg
     2041 cctgcggaga ctgaattcct ctgtcaacga cgggttctgt cttttgcacg gccggttgcg
     2101 cgctgactgg att
//
LOCUS       DATDUN010000612         3025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_565014, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000612 DATDUN010000000
VERSION     DATDUN010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565014"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..713
                     /locus_tag="VJC18_08610"
     CDS             42..713
                     /locus_tag="VJC18_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45483.1"
                     /translation="MNKILIIVMLVVVFFYVGCTQTGSSSSGGGSQNLSNGSGDATDI
                     TEITPATSKLKDSYEVSSFSEYVFFHKEDTNILLDYNFFPAGSDICQQAAYDAQTNPN
                     GCKQDKTVELPCGYQEAPEDDLLSISLNADPNIATTDCADKIILDMSQAEFTVEGLVF
                     SYDDTLDVFVEDFEFMIAKKGFVDAASDAVLVFDSSEAGTNAPQDIGLETVYQKIISL
                     RVGNP"
     gene            871..1206
                     /locus_tag="VJC18_08615"
     CDS             871..1206
                     /locus_tag="VJC18_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HLD45484.1"
                     /translation="MTIFAKIIDKEIPANIVYEDDLCLAFRDINPKAPTHILLIPKTT
                     DIDRIANARADHKELLGHMMLVCRDIAKKEGLNDYRLVINNGEGVGQTVFHLHIHIMG
                     GRAFTWPPG"
     gene            1432..2904
                     /locus_tag="VJC18_08620"
     CDS             1432..2904
                     /locus_tag="VJC18_08620"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLD45485.1"
                     /translation="MNKTIFFICVIGALIVSSLSVAQEYDPYTLPGFHKYQQGRLGSR
                     QQARVNEMQKKYPDGYAVPGPLSYAGGDFKSWQDKFYAEFVSLSGLNEKEIASKAKRF
                     SQAWIFPFDFSGGKKNILNKLLKPMKEGPLRQAYDETGEYDCKSEDARGRPLYSDYYP
                     PRYKAAKDHIELFSCRSDHEGIRVYKVGYTGFEGHKISAYLFVPNNAKNAFYQQAKGK
                     VPTLIYYHGHESTKEDAAFNPKSYVRGIAYHAALNGFLVLAPDVRGFGESKHEENHQA
                     IADRLHRQGSHFLAAAAMDAIYAQDFLENANLREFGLKYRIDENFNAIGGVSMGGLIS
                     LMAGALDKRFEVVSMQGIFLGYEVLFSKFHCPCSKMPRLLDQANIFDVALLVTPRTLH
                     VGMGAKDPFYNNYAKSAIEMFAYDLTEAGYPICYGRNDESGLGFLRNMSVRDKFQAGV
                     ECPLVLEIDKEASHEVIGYGYQFHLFWHDYLSKLSTKGFR"
BASE COUNT          763 a          660 c          790 g          812 t
ORIGIN      
        1 cgtctctttc tggtatctaa attgcatgat agaatgatgg tatgaacaaa atactcatca
       61 tcgttatgtt ggttgtggtg tttttctatg tgggttgtac acagacgggt tcgagttcct
      121 ctggtggggg atcacagaat ctttccaatg gctcgggcga tgcgacagat atcaccgaga
      181 tcactccggc cactagcaag ctgaaggaca gttacgaggt cagcagtttt tcggagtatg
      241 ttttttttca taaggaagat accaatattc tgctcgacta taattttttt ccagcgggga
      301 gcgatatctg ccagcaggcc gcctacgatg cccaaacaaa tcccaatggg tgcaaacagg
      361 ataagacagt ggaattgcct tgcgggtatc aggaggcccc agaagatgat ctgttgtcga
      421 tcagcttgaa cgcagatccc aacatcgcca caacagattg tgcagacaaa atcattttgg
      481 acatgagtca ggcagagttt acagtcgagg ggttggtgtt ctcctatgac gatactctgg
      541 acgtttttgt ggaagatttt gaattcatga ttgcaaaaaa agggtttgtt gacgccgcca
      601 gtgatgccgt actggttttt gactcaagcg aggcagggac caatgcaccg caagacatag
      661 gtctggaaac ggtgtatcaa aaaatcattt ctctcagggt ggggaatcca taattgtaag
      721 tcacattatt ctgatgatcc atataccctt caagggcaca ggtaccctca agggcacagg
      781 cgattcacga ccttaagccc tgcgccctta gccttaaacc ttgcgtatta ttgtcttgag
      841 catatttgat tcatttggca atagagtctt atgaccatat ttgccaagat catcgataag
      901 gaaattccag ccaacattgt ttacgaagac gatttgtgtc tggcctttcg cgacatcaac
      961 cccaaggccc ccacacatat tttactgatc cccaaaacga ccgacatcga tcgcattgcc
     1021 aatgcccgtg ccgatcataa agagcttttg ggacacatga tgttggtctg ccgcgatatc
     1081 gccaaaaagg aaggcttgaa tgattatcgt ctggtcatta acaatggcga gggcgtcgga
     1141 caaaccgtat tccatctgca cattcacatc atgggggggc gggcttttac ctggccgccg
     1201 gggtgagggg ggggaacgaa aggacgaggg acgaaaggac gagggacggt catagtccaa
     1261 agaccaaagt ccaaagtcgg gagcgggtaa cgggtataag gcaaatgaat attgaatatc
     1321 gagcaaggaa tatcgaatgt cgaaattcct tgttccttat tcgatattcg atcccccttt
     1381 gagctttgag cttttggctt gctatgcgat ccacacgaac aggtgatcat aatgaataaa
     1441 actatttttt ttatttgtgt tattggtgcg ctgattgtct ctagtttgtc cgtggcgcag
     1501 gagtacgatc cctacaccct tcccgggttt cacaaatacc agcaaggtcg cttgggttct
     1561 cgccagcagg cccgtgtcaa cgagatgcaa aaaaaatatc ccgatgggta cgctgtgcca
     1621 ggcccgctca gttatgccgg gggcgatttt aaatcctggc aagacaaatt ttatgcagaa
     1681 tttgtctcgt tatcggggtt gaatgaaaaa gagattgctt ccaaggcaaa acgcttttct
     1741 caggcgtgga tttttccttt tgatttttct ggagggaaga aaaacattct caacaaattg
     1801 ctcaagccca tgaaggaagg ccctttacgg caggcctacg acgaaacagg agagtacgat
     1861 tgcaagagtg aagatgccag gggaagacca ttgtattccg attattaccc cccacgctac
     1921 aaggcggcca aggaccacat tgagctgttt tcgtgtcgat ccgatcatga aggcattcgc
     1981 gtgtacaaag tgggttacac aggctttgag ggacacaaaa tttccgccta cctttttgtc
     2041 cccaacaatg ccaaaaacgc tttctatcaa caagccaagg ggaaggttcc cacactcatt
     2101 tattaccacg gtcacgagtc caccaaagaa gatgccgcgt ttaaccccaa gagctatgtg
     2161 cgtggcatag cgtatcacgc ggcgctaaac ggttttctgg tgctggcccc tgatgtccga
     2221 ggttttggcg agagcaaaca cgaggaaaat caccaggcga ttgccgatcg tctgcatcgg
     2281 caagggtctc attttctggc cgcggcggcc atggacgcca tctatgcgca ggactttttg
     2341 gaaaatgcca atctgaggga gtttgggtta aaatatcgga tcgatgaaaa ttttaatgcc
     2401 attggtggtg tgagcatggg cggactgatc agcctcatgg cgggcgccct ggataagcgg
     2461 ttcgaggtgg tgagcatgca gggtatcttt ttggggtacg aagtgttgtt ttcgaagttt
     2521 cattgtcctt gctcgaagat gccgcgtctg ctcgatcagg cgaatatctt tgatgtggcc
     2581 ttattggtga ctccgagaac cctgcatgtg gggatggggg caaaggaccc gttttataat
     2641 aactatgcca aatcagctat tgagatgttt gcctatgatc ttacggaagc gggttacccc
     2701 atctgttatg gtcgcaacga tgagtctggt ttgggttttt tgagaaacat gagtgtgcgt
     2761 gataaatttc aggcgggggt tgagtgtcct ctcgtgttgg agatcgacaa ggaagcgtca
     2821 cacgaggtca ttggttatgg gtaccagttt catttgtttt ggcatgatta tttatccaaa
     2881 ctgtcaacaa agggttttcg atgagaatcg tgttgtccct cgccgccctg ttcgttgtca
     2941 tgttctcttt ttctgaggcg tctgccgccg attgggaaac gttgcggccc gggatgcatt
     3001 ataaggtctt caaagtggat gatat
//
LOCUS       DATDUN010000613         2856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_71824, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000613 DATDUN010000000
VERSION     DATDUN010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71824"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VJC18_08625"
     CDS             <1..1449
                     /locus_tag="VJC18_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HLD45486.1"
                     /translation="GALFKIAVSVLGVFKGTMEWGIRIGKGAFAFGSDVSPMLLAVGF
                     IVGFEASTVLMVGNVISNIFAIPALSWGTDFTQTPALDVVGDVWNTQIRYLGIGAMVV
                     AGVYSIIKISGSMSSAIKTSWRGIRGLEDTASLPRNEQGISGKLLFSLLLGSMILSGI
                     VYYMMTKSMLTTGVITVLLFILAFFFVAVASYIVGLVGSSNSPVSGMTICTVLITAGL
                     FLIMGYTGPAGMLATLGVAGVVCCAACTSGDICQDLKIGQIVGATPKKFQIAEILGAV
                     SAAFIIAPTMTLLHKAYGIGTAARAGVPPLKAPQGVMFQKLVGGLFGAEAQIPWNLVL
                     VGALICVIAIIIDRYVLAPRNGKFRLYPMPLAVGMYLPMSVILPMFIGGVVYEVVAHR
                     LKKKGLSEEEQQAGVHRGLLFSSGLVAGEAIMGIFIAVLMVMNCEIPWLKNPYANSFG
                     DEWLGNIVSVIGFALMTLILMRKCFDKDRAVK"
     gene            1446..2324
                     /locus_tag="VJC18_08630"
     CDS             1446..2324
                     /locus_tag="VJC18_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLD45487.1"
                     /translation="MKSVKHKQLVSFDKTLIGYDIIGRGPEAIVLCHGLGGSLIAWSP
                     FYEAFKDKYRFITWDYRGLYSSQKPKDTTKLTIDDHCRDLALILKKEKVTRAVFAGWS
                     MGVQVCLEFFRKHARLYKGMILINGTSGYPYHAAFNSPLTRYIIPKTNQLLRKLGPKL
                     QPKLKPIAKFVIDSDDFIKIVAKLGMIHHNLDSKIFRQVAKDVVNTDLSLYTQLLDRL
                     AEHDASDVLAKINVPTLIIAGTKDVMTPSHIARGMAEQIKDSELFILNNASHYSLMEF
                     PDLINERISQFLSNLK"
     gene            2557..>2856
                     /locus_tag="VJC18_08635"
     CDS             2557..>2856
                     /locus_tag="VJC18_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLD45488.1"
                     /translation="MELKTDILIIGTGIAGLSLALKVADHFDVVLVTKREPDQSNTRY
                     AQGGIASVMASQDNFATHIRDTLNAGAHLNDPAIVEKVICAGPRLIHELSGLGVCF"
BASE COUNT          654 a          742 c          708 g          752 t
ORIGIN      
        1 ggggccttgt tcaaaatcgc tgtttccgta ttgggggtgt tcaagggcac catggaatgg
       61 ggaattcgca ttggcaaggg ggccttcgct tttggctccg atgtctcccc catgttattg
      121 gctgtcgggt tcattgtagg attcgaagcg tcaaccgtat tgatggtggg caatgtcatt
      181 tcaaatatct tcgccatacc cgctctctct tggggaactg atttcaccca gaccccagcc
      241 ctcgatgtgg tgggcgatgt ctggaacacg caaattcgct atcttggcat tggcgcgatg
      301 gtggtggcgg gcgtttattc gatcatcaaa atttctggca gtatgtcttc ggcaatcaaa
      361 acatcgtggc gcggtatccg cggcctcgaa gacacagcca gcctgccgcg caatgaacaa
      421 ggaatctccg gcaaactgtt gttctcgctt ttgttgggtt cgatgattct cagtggtatc
      481 gtgtactaca tgatgaccaa aagcatgctc accacaggcg tgatcactgt gttgcttttt
      541 attttggctt ttttctttgt cgccgtggca agctatatcg tgggtctggt gggatcttct
      601 aacagtcctg tgtcgggcat gaccatctgc actgtgttga tcactgccgg tctgttcctg
      661 atcatgggtt acaccggtcc ggcgggaatg ctggccactc ttggcgttgc gggtgtggtc
      721 tgttgcgccg cctgcacgtc tggtgacatc tgtcaggatt taaagatcgg tcaaatcgtg
      781 ggggccactc ccaaaaaatt ccagattgcc gaaattctgg gtgccgtgtc cgccgctttc
      841 atcatcgccc caaccatgac gctactgcac aaggcgtacg ggatcggcac agccgcccgt
      901 gcgggagttc ctccgttgaa agcccctcaa ggtgtgatgt ttcaaaaact ggtgggtggt
      961 ttgtttgggg ccgaagcgca aatcccgtgg aatctggttt tggtcggtgc cctgatctgt
     1021 gtgatcgcca tcatcatcga ccgttatgtc ttggcccccc gtaacggcaa attccgcctg
     1081 taccccatgc cattggcggt tgggatgtac ttgcccatgt ccgtcatcct ccccatgttt
     1141 atcggtgggg ttgtgtacga agtcgttgcc catcgcctca agaagaaggg cctgtcggaa
     1201 gaagaacagc aggctggcgt gcaccgcggg ctcctgtttt catcaggact ggtggctggt
     1261 gaggcgatca tgggcatctt cattgccgtg ctcatggtca tgaattgcga aatcccgtgg
     1321 ctcaaaaatc cctacgccaa cagctttggt gatgaatggc tgggcaatat tgtctccgtc
     1381 attggtttcg cattgatgac cctcatttta atgagaaagt gctttgacaa ggatcgtgcc
     1441 gtcaaatgaa gtccgtcaag cacaaacagc ttgtgagttt tgataaaact ctgatcggtt
     1501 acgacatcat tggtcgcgga cctgaggcca tcgtgctgtg tcatggtctg gggggatcat
     1561 taatcgcctg gtcccccttt tacgaggcct tcaaagacaa gtaccgtttc atcacctggg
     1621 actatagggg tctgtattct tcgcaaaaac caaaagatac caccaaactc accattgacg
     1681 atcattgcag agatctggct ctcattttaa aaaaagaaaa agtgactcgg gctgtttttg
     1741 ccggctggtc tatgggggtg caggtgtgcc tggaattttt ccgcaaacac gcgcgtctgt
     1801 ataagggaat gatcctcatc aacggcacca gcgggtatcc ctaccacgca gcctttaatt
     1861 cccccctgac ccgttacatc atccccaaaa caaatcagtt attgagaaaa ctggggccaa
     1921 agctgcaacc caaactcaaa cccattgcca aattcgtcat cgacagcgat gatttcatca
     1981 agatcgtggc caaactgggc atgatccacc acaatctgga ttcaaaaatt ttccggcagg
     2041 ttgccaaaga cgtggtgaac acggatttgt ctttatatac gcaactactc gacagactcg
     2101 ctgagcacga tgcctcggat gtattggcca agatcaatgt ccccacgctc atcatcgccg
     2161 gcaccaagga tgtcatgacg cccagccaca tcgcgagagg catggccgag cagatcaagg
     2221 attctgaatt gttcatcttg aataacgcgt cccattattc tctcatggaa tttcccgacc
     2281 tgatcaatga aaggatatct caatttttga gcaacttaaa ataaatcaaa aactcgggag
     2341 caagctcccg agtttaatcc tgagcgaccc ttcgactcgt tccactcgct cagggttgat
     2401 cctttttcta tttcggcacc aaggtgccga gtttgagtcg aaggatcaat gatcgatact
     2461 caatgatcaa caactcaaat tttcaattga ttgttgccca ttgaagcagt tgaagcaatt
     2521 gcaaccataa cccataactc attacccatt accattatgg aactcaaaac cgacatcctg
     2581 atcattggca ccggcatcgc gggactcagc ctggccctta aggttgctga tcattttgat
     2641 gtcgtcttgg tcaccaagcg agaacctgat caatccaaca cacgctacgc acaaggcggc
     2701 attgcctcgg tcatggcttc gcaggacaat tttgccactc atattcgcga cacccttaac
     2761 gcaggcgcac atctaaacga tccagccatt gtcgaaaaag tcatctgcgc cggtccacgc
     2821 ctcattcacg agttgtctgg tttgggggta tgcttt
//
LOCUS       DATDUN010000614         1542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_363148, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000614 DATDUN010000000
VERSION     DATDUN010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363148"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VJC18_08640"
     CDS             complement(<1..541)
                     /locus_tag="VJC18_08640"
                     /inference="COORDINATES: protein motif:HMM:NF044960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HLD45489.1"
                     /translation="MNPKQNIKSLSLQDLQNELKTLGLEGYRASQVKRWLYKFDVDSF
                     AKMTNIAGKFREILKEQFDLLRLPVVKMQLSKDGTKKYLLQLEDGHCVEVVLIPADGR
                     NTLCVSTQVGCGMCCAFCLTGTMGLIRNLTQFEILEQVSAIRRTLAEGERITNLVFMG
                     MGEPLANTASLYPAIDILLD"
     gene            complement(904..1401)
                     /locus_tag="VJC18_08645"
     CDS             complement(904..1401)
                     /locus_tag="VJC18_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45490.1"
                     /translation="MPENDAHVLADTNVIIEAHRNNCWKALVGHYKMDTVEKCIEECA
                     TGNQRLRNPVPIDTVGLEQTLVPKKVSQYQINVLYTQCKEACYLDAGEKHLLAHCLTL
                     DDVYLVCSPDKMCMQAGYALGIVDKFVSLEELIEGTGKRAQVSVPFTRRWTEQFRTRL
                     KLGLK"
BASE COUNT          386 a          392 c          313 g          451 t
ORIGIN      
        1 cgtcgagcag aatgtcgatc gcggggtaca aactggcggt gttggccaaa ggctctccca
       61 tccccatgaa cacaagattg gtgatgcgct ccccctccgc caatgttctg cgaatggcag
      121 agacctgttc gaggatctca aactgggtca ggttgcggat gaggcccatc gtgccggtca
      181 ggcaaaacgc gcaacacatg ccgcaaccca cctgggtgga aacacaaagt gtgttgcgcc
      241 cgtccgctgg gatcagcaca acctccacac aatgcccgtc ttccaactga agcaggtatt
      301 tttttgtgcc gtctttggac aactgcattt tgacaacagg gagccgcaac aagtcaaact
      361 gttcttttaa aatttcccgg aatttgccag cgatgttggt cattttggca aaggaatcca
      421 cgtcgaactt gtacagccat cgtttgacct ggctggcgcg gtaaccctcc aaaccgagtg
      481 ttttcaactc gttttgaaga tcctgtagag aaagggactt gatgttttgt ttgggattca
      541 ttattttcct aaaatgccta ttaaacagcc tgtgtcagaa aacgacaaaa aactcatcat
      601 catcatgttc ctgctggctg gcctaggcct cttcttttcc attctcctgc catttttgtt
      661 gtagtgtgat tgttttattc tcattgaaat gtttctcctg tttgttgccc attttgatgt
      721 tttctcaata tcagacctct ccaaatacat ttggatattc tttaaacttg gcaatccaat
      781 catctttgtg gtggtacaca atgaccagat aacctttgtc ctccaacttc tttcttgtat
      841 actcttacta catttggttc gtctattcga taagttgttt gacacaattt acaaaggcat
      901 cattcatttt aaccccagtt taagacgggt ccgaaactgc tccgtccacc tgcgagtaaa
      961 gggcacactc acctgcgccc ttttgccagt accctcaatc aattcttcaa gcgacacaaa
     1021 cttatcaaca atgcccaacg cataacccgc ctgcatgcac atcttgtcgg gcgaacaaac
     1081 tagataaaca tcatcaaggg tcagacagtg cgccaataga tgtttttcgc ccgcatcaag
     1141 ataacacgcc tccttgcatt gtgtataaag gacattgatc tggtattgac tcaccttttt
     1201 tggaaccagt gtctgttcaa gccccaccgt atcgatggga acaggattac gaagtctttg
     1261 gttacctgta gcgcactctt caatacattt ttctacagtg tccattttat aatgaccgac
     1321 gagagccttc cagcaattgt tgcggtgcgc ttcaataata acgttagtgt ctgccaatac
     1381 atgtgcatca ttttctggca tcattagtct cttgcaagtc aaaagggttt tttagaccat
     1441 aactcatcac aatctcttga agatcatcga ttgtgcaatc aagcaggact gcaaccttgc
     1501 gcacactcac cagacccttg tcgaggatcg tgtggagttt ct
//
LOCUS       DATDUN010000615         1101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_610666, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000615 DATDUN010000000
VERSION     DATDUN010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610666"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1101)
                     /locus_tag="VJC18_08650"
     CDS             complement(<1..>1101)
                     /locus_tag="VJC18_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HLD45491.1"
                     /translation="VELSDYIRQSGHDDSGTLARDLLASIEEDWRVEKARVTGASLKP
                     KEALIKDILNDPELGARVGVFAKDHKLKPEDCLRDVQRYLDEVGADVNYSYLQFAHIT
                     LRHLWTKIFDGVSVKPDQLNRIREVAGKYPVVLVPMHRSHIDYLLISDLFYEHNITFP
                     YVCGGINLNFWPVGRLIRKCGGFFIRRNFKGNYLYKEAVSAYVRALISQGHCLEFFIE
                     GTRSRTGRQLSPKMGILSMVLRAYLEGACEDLYFVPIAVNYDHILEERSYQSEGTGDE
                     KKQENAQELLKVRKVFKKKYGKVYIEFAEPISLKDYCRQHGLEQRPIDELKNEVAALA
                     FTLTDKMTHVALVTPMTLVSTAVLSLNKKAFT"
BASE COUNT          264 a          309 c          260 g          268 t
ORIGIN      
        1 tacgtaaacg cttttttatt gagggacaag acagccgtgg acacaagcgt catgggcgtc
       61 acgagggcca catgcgtcat cttatcggtg agggtaaagg ccagtgccgc cacttcgttc
      121 ttgagttcat cgatgggtcg ctgttccagg ccgtgctgac ggcaataatc cttgagggag
      181 ataggctcgg caaattcgat gtaaaccttg ccatattttt tcttgaacac cttgcgaacc
      241 ttgagcaact cctgagcgtt ttcctgcttt ttttcgtccc ccgttccctc actctgatag
      301 gaacgctcct cgagaatgtg gtcatagttc acggcgatgg gcacaaaata gaggtcctcg
      361 caagcccctt caagataggc cctgagcacc atgctcagaa tccccatctt gggagacaat
      421 tgtctgcccg tccgggaacg agtcccctca ataaaaaatt ccagacaatg tccttggctg
      481 atgagcgcgc gcacgtacgc actcacggcc tctttgtaca aatagtttcc cttaaaattg
      541 cgacgtatga aaaaaccacc gcacttgcgg atgagccttc ccaccggcca gaaattgagg
      601 ttaatgcccc cgcaaacata cggaaaggta atgttgtgtt cgtagaaaag atcggagatc
      661 aacagataat caatgtggga acggtgcatg gggacgagca ccaccgggta tttccccgcc
      721 acttcacgga tccggttgag ctgatcaggt ttcaccgaca ccccatcaaa aatcttggtc
      781 cacaagtgcc tgagtgtaat gtgcgcaaat tgaagatagc tgtaattcac gtcggcaccg
      841 acttcgtcca gatacctctg tacgtcccgc aggcaatctt ctggtttcag tttgtggtcc
      901 ttggcaaaca cccccacccg cgcgcccagt tctggatcat taagaatgtc cttgatcaag
      961 gcctcttttg gtttgagact ggcccccgtc acacgagcct tttcgacgcg ccaatcttct
     1021 tcaatgctgg cgagcaaatc gcgcgccaac gtcccggaat catcatgccc actctgtcgg
     1081 atataatccg ataactctac c
//
LOCUS       DATDUN010000616         4022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_525768, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000616 DATDUN010000000
VERSION     DATDUN010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525768"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VJC18_08655"
     CDS             complement(<1..265)
                     /locus_tag="VJC18_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45492.1"
                     /translation="MKHIKSAIVTLSVIFLVTVIVACGDNSSVAPALEDVEDVNAPAT
                     VDADTQASSTSSSSSQYEGGTQAGNPPTTPTPTIPQLICGQMSQ"
     gene            complement(313..1167)
                     /locus_tag="VJC18_08660"
     CDS             complement(313..1167)
                     /locus_tag="VJC18_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD45493.1"
                     /translation="MSKDITKIDLFDYLDYRKYLRDWSSSTKQNNRYFSYRNFAKKAG
                     FGSPNFLQRVINGQQKKLTLQSSITLASAIGLNKQETEFFKNLVAYNQATAHDERDHW
                     YKKLVRSQKYARIKPMMRAEYFYFSKWYNPVIRELIMSPGFDGTPEWLAKSIVPNITP
                     DMAHRSLKQLEELGFIKKVENDRWVQLNPLISTGAEAVDISITNYHQGVLDVIKNQMT
                     EIPANRRQITTFTLGVSKDKLEQIKRRIGDFGNEILKMVADETSSEEVVLVTIHMLPV
                     TDNNKNKI"
     gene            complement(1303..2211)
                     /locus_tag="VJC18_08665"
     CDS             complement(1303..2211)
                     /locus_tag="VJC18_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45494.1"
                     /translation="MVVLLFREVWMNLINYGDRVREGLSPWVRLPLVFLALGYTVYAA
                     ITNSGIYGYFADLQMEWFGGYHVVITFLITLGVCSIPVVCLFYLSLKFGVCKRATKVR
                     RLPWTAADVNRAFPYILGVGFIVGGLLAGGFFVVRGLAMGPLQRVELVSLSTQSPESR
                     YVEVAGHLKFDRAVTYAKDELTYSVIPLVPAGWDASKPVTVLVKVFKEWIDVDAHLLG
                     LDEKLDPQGLVSVKGLLSTNDLSHHALYSVQPALILASPYYVLDYTWSAHSDTRMGWG
                     MLIGMVCAGLIILGLWRWYCCRTVGD"
     gene            complement(2341..2964)
                     /locus_tag="VJC18_08670"
     CDS             complement(2341..2964)
                     /locus_tag="VJC18_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45495.1"
                     /translation="MLTMLLPFLSSCAKSVKEQMEEEHPGMQCGTSYEKLANEYSHEI
                     DPNEKTAVFVNHSQSGTVELTGEIDSKWDNASAHINIMTGASPERLIFFLGGELDGMP
                     LDETDGWDMEQDDYSFCGNKKNELLFDALLPLSCFNDEDKGIELPDLFMKCEADPDIE
                     PIWTPDDDAHRPIEPYFTGFDCGVYEMTGSSRDDDEFIGAFTWIRFS"
     gene            complement(3037..3729)
                     /locus_tag="VJC18_08675"
     CDS             complement(3037..3729)
                     /locus_tag="VJC18_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45496.1"
                     /translation="MSLATLIPALFLGCPPSAKDTSEIPGSVEGGNDSYSGQCSGVGM
                     STIEEDAVEGGLEIDATLLTDDRALPAYTANFNSSTWETYKIRFDGIDEVLTDGDITE
                     GLVGFALHNGLNCRGVAGNRADPMSGDMTEGEEFDTYKTSTIYVNGGCDNSARIMADA
                     LGGENLTVPIGEAHVRALVKCLAEENDIGVRTVAPAVIGGNDMRTIPVSPVSYVDSFV
                     NVLRSARSYFGL"
BASE COUNT         1115 a         1046 c          816 g         1045 t
ORIGIN      
        1 tttgactcat ttgtccgcaa atcagctggg ggattgtcgg tgttggcgtt gtgggcgggt
       61 ttccggcctg tgttcccccc tcgtactgtg atgaagatga ggaggtggag gaggcttgag
      121 tatcggcatc aaccgttgca ggagcattca catcttcaac atcttctagg gctggagcca
      181 ctgaactgtt gtctccgcag gcaacaataa ccgtaaccag aaaaataaca gagagagtga
      241 ctattgctga ttttatatgc ttcatacgcc caccaatctc cattttacgc cacggaaaac
      301 catgtcgcaa cgttaaatct tatttttgtt gttatcggtc accggcaaca tgtgaattgt
      361 aaccagaacc acctcttccg agcttgtttc atcggctacc atcttgagga tttcattgcc
      421 aaaatcgcca attctccttt taatctgttc cagtttatcc tttgaaactc ccagggtaaa
      481 ggttgtgatc tgtcttctgt tcgcgggaat ctccgtcatc tgatttttga tcacatccag
      541 cacaccctga tgataattgg taatggagat atcgactgct tctgcgcctg tgctgatcag
      601 gggattgagt tgcacccaac gatcattttc gactttttta ataaaaccaa gctcctcgag
      661 ttgtttgagt gatctgtggg ccatgtcggg cgtgatgttg ggaacaatgc tcttggccaa
      721 ccactcggga gtgccatcaa atcccggaga catgatcaat tcccggatga ccgggttata
      781 ccatttcgaa aagtaaaaat actctgcgcg catcatcggc ttgatacggg catatttttg
      841 cgaacgaacg agttttttgt accagtgatc cctttcgtcg tgagctgtgg cctggttgta
      901 ggccaccaga tttttgaaaa actcggtttc ctgtttgttc aagcctattg cagaggcaag
      961 tgtaatggaa gactgaaggg tcagcttttt ttgctgtcca ttgatgacgc gttgtaaaaa
     1021 gttgggggaa ccgaaacccg cctttttggc gaaatttcta taagaaaaat accgattgtt
     1081 ttgtttggtg ctactggacc aatcacgcag gtattttcgg taatcgagat aatcgaacag
     1141 gtcaattttg gtaatgtctt ttgacatagc tgtcttcaca cgatgaatca tactgtatca
     1201 tatttattat acgacatcaa tatgtgtttt attttgtatg tattattaaa gagttacgat
     1261 gaattatgaa tcctgcatga ttcgcgaaat gtgaaaatac atttaatcac cgactgtccg
     1321 gcaacaatac caacgccaca accccaaaat aatcagtccg gcacacacca tccctatcag
     1381 cattccccat cccattcgcg tgtcgctgtg cgcgctccac gtgtaatcca gaacataata
     1441 cggagaggct aatataaggg ccggctgaac agaatacaac gcatgatgag acagatcatt
     1501 tgtgctcaac aaacccttca cactcaccaa tccctgtgga tcaagttttt catccagacc
     1561 cagtaaatgc gcatcgacgt ctatccattc cttaaagacc ttcaccaaaa ctgtcacagg
     1621 tttcgacgcg tcccaccccg ccggcacgag agggatcaca gaataagtca gctcatcctt
     1681 tgcataagtc acagcccgat cgaatttgag atgacccgct acctccacat aacgagactc
     1741 aggagattgg gttgacagcg agacaagttc cacgcgctgt aaggggccca tcgccaaacc
     1801 acggacaaca aaaaaacctc ctgccaacaa ccctcccaca atgaacccca ctcctaagat
     1861 gtacggaaac gccctgttca catcagccgc agtccaaggc agacggcgaa ccttggttgc
     1921 tcttttacaa acaccaaatt tgagggacag gtaaaataaa cagacaaccg gaattgagca
     1981 aacccccaag gtgatcaaaa aagtgatcac aacatgatag cccccaaacc attccatttg
     2041 aaggtctgca aaataaccat agatccctga atttgtgatc gccgcataaa cggtgtagcc
     2101 caaggccaga aacaccagcg gcaacctcac ccacggggaa agcccttccc gcaccctgtc
     2161 tccataattg atcagattca tccacacctc acgaaataac aggacaacca cctgataatg
     2221 ctctgccaat ggttattata gtccttgctc acgagcgagc agggaatatt tgatgtgctt
     2281 cattggactc tcagaacaga attctgggaa taagtaaaga ctgtggtgag aagacaaaaa
     2341 tcagctgaac ctgatccacg taaaggcgcc aataaactcg tcatcgtctc gggaagaacc
     2401 cgtcatttca tacacaccac aatcgaatcc ggtaaaatag ggttctatgg gtctgtgcgc
     2461 atcatcatct ggcgtccaga taggttcaat atcaggatcc gcttcacatt tcataaacag
     2521 gtctgggagt tcaatgcctt tgtcttcatc attaaaacaa ctcaggggca atagggcatc
     2581 aaacagcaat tcattttttt tattgccaca aaaactgtag tcatcctgct ccatatccca
     2641 accatctgtt tcatccagag gcattccatc gagctcaccc cccagaaaaa aaatcagacg
     2701 ttccggcgag gctcccgtca tgatattgat atgcgcactg gcattgtccc attttgaatc
     2761 tatttctcca gtcaactcga ctgtcccgga ttgactgtgg ttcacaaaca cagctgtttt
     2821 ttcgttggga tcaatctcat gactgtattc gtttgcaagt ttttcatatg aggtcccaca
     2881 ttgcatcccc ggatgttcct cctccatctg ctctttaaca gattttgcgc aactggacaa
     2941 aaagggaaga agcattgtca acatgacagg tgtcacctta gtactcccct ggattgagag
     3001 gactcggtat tgtaaaaaat accgagccct tcatactcac agtccgaaat aggaacgcgc
     3061 actccgcaac acattgacaa aactatcgac atagctcact ggcgacacag gtatggttct
     3121 catgtcattt ccgccaataa ccgccggtgc cacagtcctg actccgatat cattctcttc
     3181 agcaagacat ttcaccagtg ccctgacgtg agcttccccg atgggcacgg taagattttc
     3241 cccgccaagg gcatccgcca tgattctcgc agaattgtcg caaccaccat taacgtaaat
     3301 ggttgacgtt ttgtaagtat caaactcttc cccctcagtc atatccccac tcataggatc
     3361 agcccgattc ccggccacac ccctgcagtt taacccgttg tgtaacgcaa agcctacaag
     3421 gccctcggtg atgtcaccgt cagtcaacac ttcatcaatg ccatcaaacc tgatcttgta
     3481 tgtttcccag gtgctgctgt tgaaattggc agtataggct ggcaaagcac gatcgtcagt
     3541 cagtaatgtc gcatcgattt caaggccccc ctcaacggca tcttcttcaa tggtactcat
     3601 tcccacacca gaacactgcc ctgaataaga atcattgcca ccctcgacac tgccagggat
     3661 ctccgatgtg tcttttgcag acggtggaca gcccaaaaat aaagccggta ttaatgttgc
     3721 caaactcata atttttcctc cccaaagttg ttgttgtcat acaagatgaa accccatgct
     3781 tcattttgct tacgcgcagg actagcacag gcaggcaggg caaactcaat agccatcatc
     3841 gtttaacacc atttcgtgaa tcatgcagga ttcacaaaat aacaaaaaga caccccatca
     3901 cgcacctttt ccaataatgt gccgataccc tgtgcaagtt ttacaaactt ttttgaataa
     3961 tcataaagga gcaaaatatg acaggaacat tcaatgcaac ggctgtaagc cagatgcctc
     4021 tc
//
LOCUS       DATDUN010000617         1697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_71934, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000617 DATDUN010000000
VERSION     DATDUN010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71934"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VJC18_08680"
     CDS             complement(<1..669)
                     /locus_tag="VJC18_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD45497.1"
                     /translation="MTLDQHPGHDRLSEKAVLHLATEGDFLSLADLANSHAKKLHGHR
                     IAYLIDCNINYTNICRTQCAFCAFSRPKGHTEAWELSKDELDKKIQNAKTRGSQRVLL
                     QGGMHPDHTLTDYEQLVAHIKQTHHIHVHAFSPPEIHFIAEKSGVTTKHVLERLISAG
                     LDSLPGGGAEILVDDVRQKISPLKCTANEWIDVMRVAHKLGLKTTATMMFGHIETWQD
                     RINHL"
     gene            complement(669..1415)
                     /locus_tag="VJC18_08685"
     CDS             complement(669..1415)
                     /locus_tag="VJC18_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HLD45498.1"
                     /translation="MKIGTVPYINGLPLTYGLTEPLVKRTPAELVNDLLCDEIDIAMV
                     PVYAILKHNLYMHPSAGVIACDGPVKSVGFFTRSFIQELAQIRSVYLDRESLTSVFLA
                     KILLKKYFDVSLYDVEFFHHDNRELADAQLLIGDKALFYDGRPAQFWDFGQLWKELTG
                     LGFMFACWASKRPLSSDEMTTLVKAKELGLVSRDKIASEFPIEQQTLVRDYLTNNIIY
                     EPSPDIKEGLKLYRSLLREYQYIESERKVA"
     gene            complement(1412..>1697)
                     /locus_tag="VJC18_08690"
     CDS             complement(1412..>1697)
                     /locus_tag="VJC18_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45499.1"
                     /translation="DYEQVWLYTKTQEDKKVTEDEIIDPVTNWPSLHRYVWAKTCQEG
                     DFFVLFDRGVVEKIVPDTSRKVYGSCNIKTHEEFIPIVNEKDQKRRRSVK"
BASE COUNT          410 a          394 c          438 g          455 t
ORIGIN      
        1 aagatgattg atgcggtcct gccaagtttc aatatgacca aacatcatcg tggctgtggt
       61 tttgaggcct agcttgtgcg cgacgcgcat cacgtcaatc cattcattgg cagtgcattt
      121 gagaggggat attttttgtc taacatcatc caccaaaatt tctgcgccac cgcccggcaa
      181 agaatccaaa cctgccgaga tcaatcgttc aagaacatgt tttgtagtga ccccactttt
      241 ttctgcgata aaatgaattt cgggagggga gaaggcatga acgtgaatat gatgtgtttg
      301 tttgatgtgg gccacaagct gttcataatc agtcagtgtg tgatcgggat gcatcccgcc
      361 ctgcaggagc acccgctggg agcctctggt ttttgcgttt tggatttttt tatcaagctc
      421 gtctttcgaa agttcccaag cctcggtgtg ccctttgggc cgcgaaaacg cgcaaaacgc
      481 gcattgtgtg cgacaaatat ttgtgtagtt aatgttgcag tcgatgagat acgcaatgcg
      541 atgtccatga agttttttgg cgtgagagtt ggccagatca gccagggaaa gaaagtctcc
      601 ttcagtcgcc aagtgaagga ccgctttctc cgataaacga tcgtgaccgg gatgctgatc
      661 gagtgtcatc acgccacttt tctttcggat tcgatgtact ggtactccct gagcagactg
      721 cggtacagtt taagaccttc cttgatgtcg ggagagggtt cgtagatgat gttgtttgtg
      781 agataatcac gcaccagcgt ctgttgttca atgggaaatt ctgaagcaat cttgtcacgg
      841 ctgacaagcc ccagttcctt cgccttgacc agtgttgtca tttcgtcgct ggaaagtggt
      901 ctcttggagg cccagcaagc aaacatgaac cccaggccgg tgagctcctt ccacaactga
      961 ccaaagtccc agaattgcgc gggccgacca tcgtaaaaca gggccttgtc accaatgagc
     1021 agttgggcat cggccagttc gcggttatcg tgatgaaaaa attccacatc gtacaggctc
     1081 acgtcaaaat atttttttaa gagaattttg gccagaaaaa cagaggtgag ggattcacga
     1141 tccaggtaga ccgaacggat ttgcgccagt tcctgaatga aggagcgtgt aaaaaaaccc
     1201 acactcttca cgggaccgtc gcaggcgata acacctgctg agggatgcat gtacaaattg
     1261 tgtttgagga tcgcataaac aggcaccatg gcaatgtcaa tttcatcgca aagcaggtca
     1321 tttaccagct cagcgggtgt acgtttgacg agcggttctg tgagaccata ggtgaggggg
     1381 agaccattga tgtagggcac ggtgccaatc ttcattttac actccttcgg cgtttttgat
     1441 ccttctcgtt gacgataggg atgaactcct cgtgtgtttt gatgttacac gatccataaa
     1501 cctttcggct ggtgtcgggt acgatctttt cgaccacacc tctgtcgaag agaacaaaaa
     1561 aatcaccctc ctgacaggtc ttggcccaga cgtagcgatg cagggagggc cagtttgtga
     1621 cgggatcgat gatttcatct tcggtcacct tcttgtcttc ctgtgttttg gtgtaaagcc
     1681 aaacctgttc atagtcc
//
LOCUS       DATDUN010000618         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_565131, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000618 DATDUN010000000
VERSION     DATDUN010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565131"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VJC18_08695"
     CDS             complement(<1..752)
                     /locus_tag="VJC18_08695"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HLD45500.1"
                     /translation="MNHTTIPHQRLRGEIVVAPDKSISHRSLILGAIAQGESHIKNLL
                     LGEDVLRTLTIINQLGIKTSHDATTITQGDELTIFGNGLSGLQKPNDDLYCGNSGTTM
                     RLMLGLLSGQSFSATLTGDESLDQRPMDRVLEPLRQMGAAFEVEHRDGKRFIKTLPRP
                     DAVVPFLKGLEYVMPVASAQVKTGIALAGLYAAGEVIIKEPFPSRNHTELMMKSMRVR
                     IKAKDREVCLNQVGKLKPLNLIIPGDLSSAAF"
     gene            complement(798..1979)
                     /locus_tag="VJC18_08700"
     CDS             complement(798..1979)
                     /locus_tag="VJC18_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HLD45501.1"
                     /translation="MKKYNNIERIRNRIDDLDLQMLKLLSARGKLVQRIGGIKKKQGK
                     QIRDPLREHAIIRGLSLNNLGPYSLEDVQLVFRDIIRSSYALESRSGCDYDSGPTVEK
                     KRSFRYNQVVLVGVGLMGASLARDLLKRGIAKKVIGLDSCPATLRYAKKNKIISDIAT
                     DVPRTLATSDLVILATPVRAIVNQIQSLAAVIPPTTLVMDVGSTKTSIVKTADRCFIA
                     GNFVACHPMTGRALSGPHSSVEGLFEGKVCLIVPGNKNQKKFLEQAKGLWEKLGARVE
                     TVSVAQHDRWLALCSHLPQVLSFVLLRTAVKKIGLKALVKVAGPGFKEQVRLAGSDAR
                     MWTDILLQNREHVLVQLTAFIAELGRMEQLIQKTDERKLFECLRNTACLQRNLLTAFS
                     R"
     gene            2125..>2655
                     /locus_tag="VJC18_08705"
     CDS             2125..>2655
                     /locus_tag="VJC18_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45502.1"
                     /translation="MSNNIDKSISDNFPFYKETEQTLLKELIVQADPKSESFLRAKNN
                     FLELLYSDLVHFYKWEATPDERVQIRKRLLNVAGLFFIVPISELRELSHRAKLTHKKL
                     VYETAPDPLRAETVEMHSYDLKYVDGFQDFYSKIMIDPNLSTLDYIAGNREKHQYPQS
                     QLTVFRKRIQDEVISKE"
BASE COUNT          712 a          701 c          575 g          667 t
ORIGIN      
        1 aaaaaggccg cgctggacaa gtctccgggg atgatcaggt tgagtggttt cagcttgcca
       61 acctgattga gacaaacctc acgatccttg gctttgatgc gaacgcgcat cgacttcatc
      121 atgagttctg tatgatttcg agaggggaac ggctccttga tgatcacctc tcctgcggca
      181 tacaaaccag ccagggcaat gcctgtcttc acctgggctg aagccaccgg cataacgtac
      241 tcaaggccct ttaaaaaagg aaccacggca tcgggcctgg gtaatgtttt gataaaacgt
      301 ttgccgtctc ggtgttcgac ttcaaatgcg gcccccattt gtcgtaaggg ttccagaacg
      361 cgatccatcg gtctttggtc cagtgattca tctcctgtca gtgtggccga aaaactctgg
      421 cccgacaaga gtcccagcat gagcctcatg gtcgttcccg aattgccgca gtacaaatca
      481 tcgtttggtt tctgcaaacc agacaaccca ttgccaaaga tcgtcagttc atccccctgc
      541 gtgatggttg tcgcgtcgtg agatgtcttg atgcccagct gattgatgat ggtgagggtg
      601 cgcagaacat cttcgcccag caataaattc ttgatgtgtg attctccctg cgcaatcgcg
      661 cccagaatta aactgcggtg cgaaatggac ttgtccggag caacaacaat ctcgcctctt
      721 agcctctgat gggggatggt cgtgtgattc atccggcaaa tatccgataa ttgtcagcga
      781 atgtccaaga aataatatca acgagaaaaa gctgttaaga gattgcgctg taaacaagcc
      841 gtgttccgga ggcactcaaa caatttgcgc tcatctgtct tctgaatcag ctgttccatt
      901 ctgcccaact ctgcaatgaa cgcggtcaac tgcaccaaaa catgttcgcg attctgcaac
      961 aaaatatccg tccacatcct ggcatcagaa cccgccaaac gcacctgttc tttaaaaccc
     1021 gggccggcca ctttcacaag agctttaaga ccaatttttt tcacagccgt tctcagcagt
     1081 acaaaagata aaacctgtgg caaatgcgaa caaagggcca gccaacgatc atgttgggca
     1141 acactcacgg tttcgacccg agcgccaagc ttttcccaaa gtccttttgc ctgttccaaa
     1201 aatttcttct gatttttatt gcccggcaca atcaaacaca ccttgccctc aaacaaaccc
     1261 tccacactgg aatgggggcc agagagggcg cgcccggtca tggggtgaca tgccacgaaa
     1321 tttccggcga tgaaacagcg atcggcagtt tttacgatac ttgtttttgt cgatcccaca
     1381 tccatcacca acgtcgtggg aggaatgaca gcggcgaggg attgaatctg attcacaatg
     1441 gccctgacag gtgtcgccag aatcaccaga tcactcgtgg caagagtcct gggcacatcg
     1501 gtggcaatat cggaaatgat cttgtttttt ttggcgtagc gcaacgtggc cggacacgaa
     1561 tccagcccaa tcactttttt ggcaattccc ctctttaata aatctctcgc caaggacgcc
     1621 cccatcaagc caacccctac gaggacaact tggttgtaac gaaaagaacg ttttttttct
     1681 acagttggtc cggaatcata gtcacagcca gagcgcgact ccaaagcgta tgaggacctg
     1741 atgatgtcgc gaaaaacaag ttgcacatct tcaaggctgt acggccccag gttgtttagc
     1801 gacaaccccc gaattatcgc atgctcccgc aacggatctc gtatctgttt gccctgtttt
     1861 ttcttgatcc ctccaattct ttgcacgagt tttccacgcg ctgagagaag cttcaacatc
     1921 tgcaaatcca aatcgtcaat acggtttcgt attctttcga tattattgta ttttttcata
     1981 acttctccca gtccggatga tttacttgaa tttcgcttat acactgacta aatgtggtgg
     2041 gcctcgttat ttaagaataa agcgtggaga aacacgccgg acctttgtac aaaaaaaaga
     2101 aataatattc actaaataaa aaacatgtcc aacaatatcg ataaatcaat ttctgataat
     2161 tttccctttt acaaggaaac cgaacaaacc ctgctcaagg aactcattgt gcaggctgac
     2221 cccaaatcgg agagttttct tagggccaaa aacaactttt tggaactgtt gtattcggac
     2281 ctggtccatt tttacaaatg ggaagccaca ccagacgaaa gagtgcagat ccgcaaaagg
     2341 cttctcaacg tcgctggtct ttttttcatc gttcccatct ctgagctgag agaactctct
     2401 cacagggcaa aactcacaca caaaaaactt gtttacgaaa ctgcccccga tcccttgcgc
     2461 gccgaaacag tcgaaatgca ctcttacgac ctgaagtacg tcgacggttt tcaggatttc
     2521 tactcgaaga tcatgatcga tcccaattta tcgacgctcg attacattgc cggcaatcgc
     2581 gaaaaacacc aatacccgca gtcacaactc accgtgttcc gcaaacgtat ccaggacgag
     2641 gtgatttcca aggaa
//
LOCUS       DATDUN010000619         1374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_363412, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000619 DATDUN010000000
VERSION     DATDUN010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363412"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1374
                     /locus_tag="VJC18_08710"
     CDS             <1..>1374
                     /locus_tag="VJC18_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45503.1"
                     /translation="VYNDYVYYYKQIGSGYEFIFRSLHDVMQQTFLYTNHIMKEVTFY
                     PDRTYFDVMYPAMIFGISNDGAIVKQTPQGEEIMNKLKTELPASGLVESYVDYAVSPN
                     GLFVMSFMRAEQGTMLLQSLAAFSETKDFPIETVLDPLVAGRSLIWLDTHNVLSYTYF
                     KQRSEYAYSDLELIDRMTKKIKRKEMTAKKAVLGNQQEGQEQAESGDDSSARHDEMIA
                     KLDMCMKISSDVCSVVELFDMNEFTNPERPDVFGIHNWTESGEVRMQAKILQVVPYRI
                     TSVAASDYVGAMTEPYFTGNSFVNGVQQIFFGSLYNGENTKTNIQCPISVLTGGEADK
                     RPPTVSKDGLLLAYETTDSSNERNIQIVYMPTREYFWLASPELEGLEFLGPKFSAESQ
                     HGLSFFVMAQDEPAMIGTVNLRTHPKLKQILDALPQNNAERQVLPLFNNCTENLVNVE
                     EVTEFKN"
BASE COUNT          366 a          266 c          358 g          384 t
ORIGIN      
        1 ggtttacaat gactatgttt attattataa acagattggg tccggttacg aatttatttt
       61 tcgatcactg catgatgtga tgcagcagac ttttttatac acgaatcaca taatgaagga
      121 ggtcactttt taccctgacc ggacctattt tgatgtgatg taccctgcca tgatttttgg
      181 gatttcgaat gatggggcga ttgtcaagca gacgccacag ggtgaagaaa tcatgaataa
      241 gttgaaaacg gagttgcctg cttcggggct tgtggagtcg tatgtggatt acgcggtttc
      301 gcccaatggt ctgtttgtca tgtccttcat gcgggccgaa caaggaacca tgttgttgca
      361 atctttggcc gcgttttcgg agaccaagga ttttcccatc gaaaccgtgc ttgacccttt
      421 ggttgcgggg cgatctttga tctggttgga tacacataat gttttgagtt atacctattt
      481 taagcaacgt agtgaatatg cttatagtga tttggaattg attgatcgga tgacgaaaaa
      541 aattaagaga aaagaaatga cggccaagaa ggctgtgctg gggaaccagc aggaggggca
      601 agagcaggca gaatcgggtg atgatagttc agcgcggcat gacgagatga tagctaaact
      661 ggatatgtgc atgaaaatct catccgatgt ttgttctgtg gttgagctgt ttgacatgaa
      721 tgagttcact aatcctgaac ggccagatgt ttttgggatt cacaattgga cagagtctgg
      781 agaggttcgc atgcaagcaa agatccttca agttgttcct tatcgaatca cgagtgtggc
      841 ggccagtgat tatgttggcg ctatgaccga gccgtatttt accggcaact cgtttgtaaa
      901 tggtgtgcaa caaatctttt ttggttcttt gtacaacggt gagaacacca aaaccaatat
      961 tcaatgtccc atcagcgtgc tgacgggggg cgaggcggac aaaagacctc ctactgtgag
     1021 caaagacgga cttttgttgg catacgaaac gaccgattcg agcaacgaaa gaaatatcca
     1081 gattgtctat atgcccacgc gcgagtattt ctggcttgcg tcgccggagt tggaaggact
     1141 ggagttcttg ggaccaaaat tttcggcaga atcacagcat ggtttgtcct tttttgtcat
     1201 ggctcaagat gaacccgcca tgatagggac ggtcaatttg cgcacgcatc ccaaactcaa
     1261 acaaattctt gatgccctgc cacaaaacaa tgcggagcgg caggtgctcc ccttgtttaa
     1321 taattgtacc gagaacctgg tcaatgttga ggaagtgact gaattcaaga atta
//
LOCUS       DATDUN010000620         1041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_282083, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000620 DATDUN010000000
VERSION     DATDUN010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282083"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1041)
                     /locus_tag="VJC18_08715"
     CDS             complement(<1..>1041)
                     /locus_tag="VJC18_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45504.1"
                     /translation="EALEVTNWKAPRGSAFVDDVLRRHPRPVSVGASGSARVENFVDA
                     DEFSFDRISFGSGDVFEGEATYGLHGLLVWQGEVAVTDLSGREWGVYRQGQPIIVPAH
                     VGQYRLIAKEQAQVFVTRSDPRSYSLRAKLAWRDKQTNPGTPASMTLKEVMVLSAVEM
                     GTSGARGLVKDMTDEDTYANTRAFLQLLESVENLQTGEQIGVAGDLRDFLNSTNRIMR
                     AVGKAIADKGLWVVNLGKVPTQAAALWGAQTGHPVIMVTGSHIPDDRNGIKFYLRNGR
                     ELLKEHEAPLKGQVVQLPWALFGDDGQFKSDVTYALPDNVEAQVRREFVDRYLSFFGA
                     GRLQGLNLGHYQH"
BASE COUNT          241 a          322 c          241 g          237 t
ORIGIN      
        1 gtgctggtaa tgacccaggt ttaggccctg taaacggcca gcgccaaaaa aactgaggta
       61 tctgtcaaca aattcgcgac gcacttgagc ttctacgttg tcgggcaagg cataagtcac
      121 atctgattta aattgaccat cgtcaccaaa taaagcccat ggcaattgaa caacctgtcc
      181 cttcaaggga gcttcgtgtt cttttaacag ttcgcgaccg tttctgaggt agaattttat
      241 gccgttccgg tcatcgggga tgtggctacc cgtcaccatg atgacaggat ggcctgtctg
      301 cgccccccac agggcggccg cctgagtcgg gaccttcccc aaattcacca cccaaagacc
      361 tttgtctgca attgctttgc caacggcccg catgatcctg ttcgtgctgt ttagaaaatc
      421 tcgcaagtca ccagccacac caatctgttc gccggtctga agattttcga cagattccag
      481 caactgcaaa aaagcccgcg tgttggcata ggtgtcctcg tcggtcatgt ctttgaccaa
      541 acctcgggca ccgctggttc ccatttcgac cgcactcaac accatgactt ctttgagggt
      601 catgcttgcg ggggtacccg gattggtctg tttgtcgcgc caagcaagtt ttgcccgcag
      661 actgtaactc cggggatcac tgcgggtcac aaacacctgg gcctgttctt ttgcgatcag
      721 tctgtactgt cccacatgcg cgggaacgat gatcggttgg ccttgtctgt acacgcccca
      781 ctctctccca ctcaaatctg tgacagccac ctcaccttgc caaaccaata acccgtgcag
      841 gccatacgtg gcctcaccct caaagacatc ccccgatcca aaagagatgc ggtcgaacga
      901 aaactcgtct gcatcaacaa aattttccac ccgcgctgaa ccactcgcac caacggaaac
      961 cggccgggga tgacgccgca aaacgtcgtc cacaaaggca ctgccacgcg gtgccttcca
     1021 attggtaacc tccaaggcct c
//
LOCUS       DATDUN010000621         3043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_525959, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000621 DATDUN010000000
VERSION     DATDUN010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525959"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1608)
                     /locus_tag="VJC18_08720"
     CDS             complement(106..1608)
                     /locus_tag="VJC18_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HLD45505.1"
                     /translation="MTNYKLNLNTVTQETYEYAKKRLLQERPDQTETIYDIFEDEQVT
                     PDEMRSLYFPQWYTTGYAGVERYEKALSRMPDALGFQLLACSPFSAFKRYNDYADKYV
                     QYNGDMDRVMHSYASFDLNGNTIFSQSQAQRPKWIMSGRLHQPYDLAEQFKEIFPSLA
                     NTVTQMFSDRALTSAEMLTLWQNCDDNVFNEEGDFATKQVSQLNDSLAWFGLDYQTLT
                     KPVPVEEPVFEPITPQLISEEILSDPDPVNQTTVATSADSIDQATDTTSEQVVTTFGD
                     AVVNTTHSDTKTELTAVTLGETTVTTERPDIVSMPVPETQTTSELPENAAPAVATTPE
                     PVINNEVFLDPGTTPFDLTTTETAQPTSAIITTNEPETGNDPADLPTAISDTLRSLGL
                     DDQPASHSKLLELLSYGWLSLSQLPDPLRANPGIVSAAVHYEGMELRYASTDLRANKD
                     IVTLAIKSNGAALMYASDDLKNNPELIKLANNQLTFSFFFEADRNQHAIA"
     gene            1886..2398
                     /locus_tag="VJC18_08725"
     CDS             1886..2398
                     /locus_tag="VJC18_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45506.1"
                     /translation="MPVSATNKTANSQVVSTSLLSTDDEDIRQRECADTPAEVFSACR
                     GCHPVVETFSNFSHLYPMNETTRQVMSSCVTTAAGFNIRSFDLFKANVSVCTEKKILL
                     DEAQKNAMGRVLDALTAEAASSTRAKAWDACAERVISAGRGATPETDQPSQTPVPSEP
                     LVPDLQTQKK"
     gene            2675..>3043
                     /locus_tag="VJC18_08730"
     CDS             2675..>3043
                     /locus_tag="VJC18_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45507.1"
                     /translation="MIEANGTEFANDESGGGIMGMTSVLKSQHTSDAVTDGRLLSDEQ
                     MRLDTCPNIPQDVFVQCGGCHPIMAAFADFERVYPMSETTRQAVSSCFKSSATFNVQT
                     LHIVNAGLNLCTEKNISLDEA"
BASE COUNT          767 a          651 c          777 g          848 t
ORIGIN      
        1 tttgtaggag aatacttcca gtaacctttg ctgagcatat tcaaaagtac tttgcgtgac
       61 ggtgttcatg ttgagctgat acgtagtcat acatattcct tgtaattaag cgatggcgtg
      121 ttgattcctg tctgcttcaa aaaagaaact aaacgtgagt tgattatttg ccaatttgat
      181 caattcagga ttgtttttga ggtcatcact ggcgtacatc agcgccgcgc cgttagactt
      241 aatcgccagc gtgacgatgt ctttgttggc tctgaggtcg gtggaggcat atctgagttc
      301 cataccctcg taatggaccg ccgcagacac aataccggga ttggcacgca ggggatctgg
      361 taattgtgaa agactcagcc atccgtacga aagaagttcc agcagcttgg aatgactggc
      421 gggctggtca tccaatccga gcgagcgcaa tgtatctgag atggcagtag gaaggtccgc
      481 tgggtcgttc ccagtctcag gttcatttgt tgtgatgatg gcagatgtgg gctgagcggt
      541 ttctgtggtg gtcaggtcaa aaggtgtcgt gccgggatcg aggaagactt cattgtttat
      601 cactggctcg ggagttgtag cgactgcggg tgccgcattt tcaggaagct cagaggtggt
      661 ttgtgtttcc ggaacaggca ttgagacaat atcaggccgc tcagtggtga cagtggtttc
      721 accaagagtg actgctgtga gttctgtttt ggtatcagag tgagttgtgt tgaccacggc
      781 atcaccgaaa gtggtcacaa cctgctctga cgtggtgtca gttgcctgat caatggaatc
      841 agcacttgtg gccaccgtag tctgatttac aggatccggg tcagacagga tttcttcaga
      901 aatcaattgc ggggtgattg gttcaaacac gggttcctca acaggcactg gttttgtgag
      961 ggtctggtaa tccagtccaa accaggcgag cgagtcattc aattgtgaca cttgttttgt
     1021 ggcaaaatct ccctcttcat tgaagacatt gtcatcacag ttttgccata gcgtcagcat
     1081 ttccgccgag gtcaaggcac gatccgagaa catctgggtg accgtatttg ccaaggacgg
     1141 gaatatctct ttaaactgct ctgccaaatc gtatggttgg tgcagtcgac ctgacattat
     1201 ccattttggc cgctgagcct gtgattgcga aaagatcgtg ttaccattga gatcaaatga
     1261 cgcgtaagag tgcatgacac gatccatatc accattgtat tgtacatatt tgtctgcgta
     1321 atcattgtat cgtttgaatg cggagaaggg tgagcaagcc agcaattgaa aacccaatgc
     1381 atctggcatt cgtgacagcg ccttttcgta gcgctcaact ccggcataac ctgtggtgta
     1441 ccattgtggg aaatacagcg aacgcatttc atcgggtgtc acctgctcat cctcaaatat
     1501 atcgtaaatg gtttctgtct gatccgggcg ctcctgcaat aatcgttttt ttgcatactc
     1561 ataagtttct tgggtcactg tgttaagatt gagtttatag tttgtcatat tttttctccc
     1621 tgtttccaca ataagcaagg cgcgtgccaa agatgagatt attatttttg tgaatgatta
     1681 tcagtgggat ttcatttatt tgacaaatgc cgattatatg agctgtgcta attccttaac
     1741 acttgtccaa attcgcacgc gataatgtcc agaaactgcg acacagatat ttcttgcctc
     1801 cggctggcgt ggccaaatta ttgagttttc tatcttggcg ggtttggcac gcgccttgct
     1861 atgttgagag gcaaggagaa tctttatgcc cgtttcagcc accaataaaa cagcgaacag
     1921 tcaggttgtt tccacttctc tcttgtcgac agatgatgaa gatatccgtc aaagggagtg
     1981 tgccgatact ccggcagaag ttttttctgc ttgcaggggt tgtcatcctg tcgtggagac
     2041 attttccaat ttttcgcatc tgtatcccat gaacgaaaca accagacagg tcatgtcttc
     2101 gtgtgtgacg acagctgccg gtttcaatat caggtcgttt gatttgttta aggccaatgt
     2161 gagtgtttgt accgaaaaaa aaatcctgct tgatgaggcg caaaagaatg ccatggggag
     2221 ggtgctcgat gcgctcactg ccgaggccgc gtcgagtaca cgcgcaaaag cgtgggatgc
     2281 ctgtgcggag agagtgattt cagccggaag gggtgcgact ccggaaacgg accaacccag
     2341 tcaaacccct gttccctcgg agccactcgt acctgatttg caaacacaaa aaaagtaaat
     2401 ctgttgccac tgtaccaaca aggagactcc catgacgacg accacaacaa agacagccgc
     2461 agctcctatc tatgtccaac caatatcatc cttgaggggg aacctatatc gcaaaatatt
     2521 tagtgtaaaa agatggggtt aatggcgcgc ttacagtgtt ggcagtctgt ccaaaatcta
     2581 aaacatgtgt ccaaggacag acacggtgat gtccaattac atcgacagat gcaaaatgtt
     2641 cattgcagtg atgtggctga atcattgacg tttattgatt gaagcaaatg gcaccgaatt
     2701 tgctaatgat gagagtggtg gaggaattat gggtatgacc agcgtgctaa aatcacaaca
     2761 tacaagtgac gccgttactg acgggcggtt attgtcagac gaacaaatgc gtttggacac
     2821 atgtccaaat atccctcagg atgtttttgt gcagtgtgga gggtgtcatc cgatcatggc
     2881 cgcgtttgca gactttgaga gggtttatcc catgagtgaa accacaaggc aggctgtatc
     2941 atcctgtttc aagtcctctg ccacctttaa tgtgcagaca ttacatatag tgaatgcagg
     3001 gcttaatctg tgtacggaaa aaaatatttc tctggatgaa gcc
//
LOCUS       DATDUN010000622         3233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_363479, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000622 DATDUN010000000
VERSION     DATDUN010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363479"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..718
                     /gene="efp"
                     /locus_tag="VJC18_08735"
     CDS             158..718
                     /gene="efp"
                     /locus_tag="VJC18_08735"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HLD45508.1"
                     /translation="MQGNDLRKGNVIKLENKLWLAMSVEHRTPGNLRAFIQAKMRSIG
                     DGTQKEFRFSSTERLEQVDIFERKMQYLYSDGDVYNFMDTENFEQIELSKETIGDYAI
                     YLTPEMNISVAFCENNPITVKLPTTMDFEVVEADPEIKGATASASYKSATLSNGLNVQ
                     VPQFVKVKDVIRINVEKGEYQERVKR"
     gene            690..1316
                     /locus_tag="VJC18_08740"
     CDS             690..1316
                     /locus_tag="VJC18_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLD45509.1"
                     /translation="MNIRNESKDRGSLDPEVLQCHNDQKHRYGELYRKYQDSPQGMHF
                     VSGHTQLSRFLQFLQMGNLEGKKVLDLGCGFGDFYKFLKEQGICVQYRGYDIVREFIT
                     CCREKFPDGHFEERDILFKKPRVKFDYVIASGVFAHANLPFFKEMIKVAYGLCRTAFG
                     FNMFDTAQEDFFHIDEEEVLHWCRQMNPADIKVTRDYLPGDYTVFVYR"
     gene            1548..1847
                     /locus_tag="VJC18_08745"
     CDS             1548..1847
                     /locus_tag="VJC18_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45510.1"
                     /translation="MRVSSVNSQPFLAHDPGLFVVNDILHIVLQNIPEELRKRCRVDT
                     QSVSRGSIEDAVLHDHWSLDELENVDYILNEGMLQVTLVFRDPFNRQQARYTFEV"
     gene            1926..2384
                     /locus_tag="VJC18_08750"
     CDS             1926..2384
                     /locus_tag="VJC18_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45511.1"
                     /translation="MRHTYLFYESEWVASGVYYNNAGTSVPFEGGSKITHQDLLWRLD
                     SRITLCRPNTIEFTNTYEITPLVDGQTETQFVSRNPSLGRFTGSLIFVEDTIFVCAHS
                     EDHSQMVIETNRKIDNNTYANHGVLLVGNVRMSSWTARLSTEAQKLKLIK"
     gene            complement(2635..>3233)
                     /locus_tag="VJC18_08755"
     CDS             complement(2635..>3233)
                     /locus_tag="VJC18_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45512.1"
                     /translation="EASQQCQDMLNDGAWGTAEAGAQFETGGDWIPNKNVLIYNNIFY
                     NPDGVSSVNGIIAVNGSVALPGDADNIPDPALADEDLSIRGNVFWNGGVASTLLTSTN
                     GSDPACEEGNGSCNENQLEDENEINSVEPELTDPADGDFTPVDGGNVTSLSANEIPNF
                     TWDTWLTNADVPEGDLDNTVTNNFLGEARGDSPHPGAY"
BASE COUNT          828 a          756 c          789 g          860 t
ORIGIN      
        1 acaatgattg tattcaattt ttcctaggcc cttagccctg cgccttacgc cttgtgccca
       61 aatgtttcgt gagcacacaa agcacaaaaa cacttgtgcc gctttgatca gaagtagtat
      121 gagcgcgcgg ggaagcaaag gcacgaaagg atgttttatg cagggcaatg atctcagaaa
      181 aggcaatgtg attaaactcg aaaataaatt gtggttggcc atgtccgtgg agcacaggac
      241 ccccggtaat ttgagggcct tcattcaggc caagatgcgc tctatcggtg acgggactca
      301 aaaagaattt cgtttttcat ccacggaacg tttggagcag gtcgatattt ttgaacgcaa
      361 gatgcagtat ctttactcag atggcgatgt atacaatttc atggataccg agaatttcga
      421 acaaattgaa ctgtccaaag agacgattgg ggactatgcc atttacctca cgcccgaaat
      481 gaacatcagc gtggcctttt gcgagaacaa tcccatcaca gtcaagcttc ccaccaccat
      541 ggatttcgaa gttgtcgagg ccgatcccga aatcaagggg gccacggcca gtgcctcata
      601 caaatccgcc acgctcagca atgggctcaa tgtgcaggtt ccgcaatttg tcaaagtcaa
      661 agatgtcata cgcatcaatg tcgaaaaagg tgaatatcag gaacgagtca aaagataggg
      721 gttccctcga tcccgaagtc ctgcagtgtc acaatgatca aaagcatcgt tacggggagc
      781 tgtatcgcaa atatcaggat tcacctcagg ggatgcattt cgtctcagga cacacccaac
      841 tgtcgcgttt tttgcagttt ttacagatgg gcaatctgga ggggaaaaaa gttctggatc
      901 tgggttgcgg ctttggtgat ttttacaaat ttctgaaaga gcaggggatt tgtgtccaat
      961 ataggggtta cgatattgtg cgtgaattta ttacctgttg cagagaaaaa tttccagatg
     1021 gtcactttga ggagcgggac attcttttta aaaaacccag ggtaaaattt gattacgtaa
     1081 ttgccagcgg tgtctttgct cacgccaatt tgccgttttt taaagagatg atcaaagtgg
     1141 cctatggttt gtgtcgcaca gcctttgggt tcaatatgtt cgacacagct caggaagatt
     1201 tttttcacat tgatgaggaa gaagttcttc actggtgccg tcaaatgaat cccgctgaca
     1261 tcaaagtcac cagggactac ctgccggggg attatacggt gtttgtgtat cgatgaatca
     1321 ccgtagctca aagctgaaag ctcaaagctc aaagctcaaa ggttgaagtt ggacaaacaa
     1381 accttatttg ttcaactgtc aggatttttt ctttgcttgt cagaaacgca acagcttgcg
     1441 atatcatttt ctgacaatta tcaaaaaata tcttgttgtg aataattctg ttctgtacaa
     1501 tagaactgtc ggaataaaaa actttgctct gaatcatgag agcctctatg cgtgtttctt
     1561 ctgtcaactc gcaacccttt ctcgctcatg accccggtct ttttgtcgtc aacgacatcc
     1621 tgcacattgt gcttcagaat attcccgagg agcttcgtaa acgctgtcgt gtcgacacac
     1681 agagtgtttc tcgaggatct attgaagacg ctgttctgca cgatcactgg tcgctcgatg
     1741 aactggaaaa tgtcgattat atccttaacg agggcatgtt gcaggtgacc ctcgtgttta
     1801 gggacccctt caatcgccag caggcgaggt atacatttga ggtatgaggc acaaggcgta
     1861 aggcttaggg gataacggaa gatcctgttc ttgctttgcc gaacacatca cgctagatgt
     1921 tgagcatgcg gcacacatat ctcttttacg aatccgaatg ggtggcaagc ggtgtgtatt
     1981 acaacaacgc gggcacgtct gtgccgtttg aagggggttc caaaatcaca catcaggatc
     2041 ttttgtggag attagacagc agaatcacgc tgtgccgacc caacaccatt gagttcacca
     2101 acacatacga aatcacgcca ctcgttgacg gccaaacaga gacgcagttt gtgtcgcgca
     2161 acccgtctct gggcaggttt accggttcac tcatttttgt tgaagacacc atctttgtgt
     2221 gcgcacacag cgaagatcat tctcagatgg tgatcgaaac caatcgcaag atcgataaca
     2281 acacttatgc caatcacggg gtcctgcttg tggggaatgt caggatgagt tcgtggacag
     2341 ccaggctttc aaccgaggcg caaaaactta aactcataaa ataaaaaaag cccgctctcg
     2401 gtttgccgtg agcgggcttt tttgtgaact gcttacttgc agcaacagcc ttttttcttg
     2461 gctggctttt tgactgcttt tttcttggct ggcataaagc tccttttggt tgtgggttga
     2521 taacaatcta caatttgggc actctattcg ctttggcaac aaaagacaaa ttaatagtag
     2581 gggtcggtcg cgaccgaccc ctacgggttt cgagatcatc aaaataattg atcctcaata
     2641 cgcccccgga tggggagagt caccgcgtgc ttcacccagg aaattgttgg tcacggtgtt
     2701 gtcaagatca ccctcgggga catccgcgtt tgtgagccaa gtgtcccagg taaaattcgg
     2761 aatttcattc gcgctcaaac tggtgacatt gccgccgtcc acgggagtaa aatcgccatc
     2821 tgccgggtcg gtgagttcgg gctcaaccga attgatttcg ttttcgtctt cgagttggtt
     2881 ctcattgcaa ctcccgttgc cctcttcaca tgccgggtcg gaaccattgg tgctggtgag
     2941 taatgtgctc gccacaccac cgttccagaa cacattgccc ctgatggaaa ggtcttcatc
     3001 cgccagcgcg ggatcgggga tgttatccgc gtctccggga agggccacgg aaccattgac
     3061 cgcgatgatc ccgttgactg agctcacccc atcaggattg taaaagatgt tgttatagat
     3121 caaaacattc ttgttgggga tccagtcccc gcctgtttca aactgagcac cggcctcggc
     3181 agtgccccaa gcgccgtcgt tgagcatgtc ctggcactgt tgggatgcct ccg
//
LOCUS       DATDUN010000623         2096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_152682, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000623 DATDUN010000000
VERSION     DATDUN010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152682"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1908
                     /locus_tag="VJC18_08760"
     CDS             253..1908
                     /locus_tag="VJC18_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLD45513.1"
                     /translation="MANSPKLNWKKSTLDPFAAKNPERKNRFATISDLEIDRLKSEED
                     LAGFTQDGDLGFPGAFPFTRGVYPTMHRGRLWTMRQFAGFGSAGDTNKRFKYLLEHGQ
                     TGLSTAFHFPTLMGYDSDDARSLGEVGKCGVAIDSLKDMETLFDGINLEKVTVSMTIN
                     GPAAIMLAFFIATAQKQGRDLKKVGGTIQNDILKEYIAQNSYLIPPEPGIKLVVDTIE
                     YCTQHLPKWNPVSISGYHIREAGSTAVQELAFTLYDGLEYVKHCVARGLDVDSFAPRL
                     SFFFNAHADFFEEIAKYRSARRIWAREMQRRFDPQNSHSLRCRFHVQTAGCSLTAQQP
                     ENNIVRTTLQALSAVLGGCQSLHTNSMDETLALPTEKSVMIALRTQQIIAFESGVTNT
                     IDALGGSYFVESLTNQMEQDAMTIIKKIDEMGGMVNAIKNGYPMRAIAEASRHYQSQV
                     ERREQTIVGLNEFMIDSEPPIETLKIDPTVEHKQKSAVQALRKTRDNTLCEKHIFTLR
                     KACQNTHNVMPALIDCAHAYCTIGELAKVLREEFGEYRDPGIF"
     gene            1991..>2096
                     /locus_tag="VJC18_08765"
     CDS             1991..>2096
                     /locus_tag="VJC18_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HLD45514.1"
                     /translation="MKSKIRILIGKPGLDGHDRGAKIVARCLRDAGFEV"
BASE COUNT          525 a          574 c          514 g          483 t
ORIGIN      
        1 ggacatgcct tcgtcatctt cgcaaaaagt cctcaacgtg gcgtgcctta cacgtctccg
       61 ggctttttac tcagcttcct tggcctgtcc aaaaatacct gcgcctttgt aggtccttat
      121 taatacagga ctcagtaagg gcaccttaat cccttgattc tcactttgtc atgagttggg
      181 ggtaaacaga accaatcctg ccgaaactaa ctccttgacg ctggttttgt tcttcattta
      241 tcgtgaccgg acatggccaa ttcaccaaaa ttaaattgga aaaagagtac cctggatcct
      301 tttgccgcaa aaaatcctga gcgcaaaaac aggtttgcca ccatctcaga tctcgagatc
      361 gatcgcctca agagcgagga agacctcgcc ggattcacac aggatggtga tttgggtttt
      421 cccggagctt tcccttttac acgcggggtc taccccacca tgcaccgcgg tcgtttgtgg
      481 accatgcgtc aatttgcggg ctttggttcg gcgggcgaca ccaacaagcg tttcaaatat
      541 cttctggagc acgggcaaac aggcctctcc acggcgtttc attttcccac actgatgggg
      601 tacgattccg atgatgcccg aagcctggga gaggtgggca agtgcggtgt ggccatcgac
      661 agccttaaag acatggaaac cctgtttgac ggcatcaatc tggaaaaggt cacggtctcc
      721 atgaccatca acggccctgc cgcgatcatg ctggcctttt tcatcgccac ggcgcaaaag
      781 cagggacgcg atcttaaaaa agtgggcggc accatccaga atgacatcct gaaagaatac
      841 attgcccaga actcttacct catcccgccg gagcccggaa tcaaactggt ggtcgacacc
      901 atcgaatatt gcacacaaca cctgcccaaa tggaaccctg tatcgatctc cggttatcac
      961 attcgtgaag cgggctccac agccgtacag gaattggcct ttacacttta cgatggtctg
     1021 gaatacgtca aacattgtgt ggccagaggg ctggatgttg attcttttgc cccacggctt
     1081 tctttctttt ttaacgcgca tgcggatttt tttgaggaga tcgccaaata ccgctctgca
     1141 cgccggattt gggccagaga aatgcagcgc cgtttcgatc cccaaaattc ccattctctc
     1201 cgttgccgtt ttcacgtgca gaccgcgggt tgttcgctta ccgcgcaaca gcccgaaaac
     1261 aacattgtcc gcaccacctt gcaggccctc tctgccgtgc tcggcggttg ccagagtctg
     1321 cacaccaatt cgatggatga gaccctggca ctccccaccg aaaagtctgt gatgatagcg
     1381 ctcagaacgc aacagatcat cgcctttgaa tcgggcgtga ccaacacgat cgacgcgttg
     1441 ggagggtcgt actttgtgga atccctgacc aaccagatgg aacaggacgc catgaccatc
     1501 atcaaaaaaa tcgatgagat gggcggcatg gtcaacgcca tcaaaaacgg ctaccccatg
     1561 agggccattg ccgaggccag ccgccactat cagagtcagg ttgagaggcg cgagcagacc
     1621 atcgtgggcc tgaatgaatt catgatcgat agcgaaccac caatcgaaac actaaagatt
     1681 gatcccacag tggaacacaa acaaaaatca gctgtgcaag ccttgcgcaa aacgcgtgac
     1741 aacacccttt gtgaaaaaca catttttacc ttgcgcaagg catgtcaaaa cacgcacaat
     1801 gtcatgcccg ccctgatcga ttgcgctcat gcgtactgta cgatcgggga gttggccaaa
     1861 gtgctccgag aggaatttgg tgagtacagg gacccgggga tattttgaat cgtgaatcgc
     1921 agatcgtgaa tcgtggatcg tataagatat tttcgatcca cgatccacga tctgcgattt
     1981 acaactgctc atgaaatcga aaatccgcat cctcattggc aaacctggtc tggacggtca
     2041 cgaccgcggt gccaagattg tggctcggtg cttaagagac gccggctttg aggtga
//
LOCUS       DATDUN010000624         2746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_152764, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000624 DATDUN010000000
VERSION     DATDUN010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152764"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2746)
                     /locus_tag="VJC18_08770"
     CDS             complement(<1..>2746)
                     /locus_tag="VJC18_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HLD45515.1"
                     /translation="EDGRLTERLYAEYNELNLSQAPRITHVSSTQEAMTLLKERRFDL
                     VMTMVRLEDTNVNAFGRMVKSIVPDMPVVLLIFNESDVDHFPGGIDREAIDYPFVWRG
                     DARILLTIIKLIEDKRNIDHDIKSAGVRCIIVVEDSVSRYSSFLTMLYSVVMSQSNSL
                     ISEGLNDLHKIYRMRARPKIVLATSYEEAVGHYERYKHSVLALISDVNFPKDGKRNYE
                     AGFELAAYVHRDNPNLEVLLQSALERNEARAREMGLMYVDKNSPHILRSLRNFLIQNL
                     GFGTFIFKMPDGEEIARAQDLYDMERIIMTIPPVSLEFHATRHHLSSWFCARSLFVLG
                     DELRSYSVSDFKSIEDIREFLLKKIKESRDCEQQGLITDYSSRQAGPESLFVRMGRGS
                     IGGKGRGIAFLNTLFARARIKKLGKLLPIRIPKSVCFGTDEFERFMESNHLSDVVQNA
                     ANDAELIKAFLKASLSDEHWEHLRFVLHNMPGPLAVRSSSILEDSHIQPFAGIYATYM
                     IPNNHADHQVRFKELRSAIKAVYASLYSQNARSYIANTPHLTEEESMAVVIQQVVGQA
                     HGHRYYPLISGVAQSYNYYPIGHQKAQGGIAQIAMGLGYGVVSGGSVMRFSPSCPEVL
                     PQYAKPEDYLKYSQKEFYALDLSKQQVDFMLSPEVALSRCQLEDAEQDKTLQQVGSVY
                     HVQDHTIRDNFGLPGPRVITFNNILKWRDIPLAEVLAELLDIIRQEAGFAVEVEFALD
                     VHAETGPALYVLQMRPLAMQLVHGAVETVGFDQKDVLCRTDLSLGHGVLKDIRDIVYV
                     KPNQFDGTKTRQVAEHIGRMNSLLQTEGARYFLIGPGRWGSSDPSLGIPVEWSQISGA
                     RLIAETSFRDQRVEPSQGTHFLQNIVARGVGYLTLSQPVTARGLSVAYLDWDWLEQQT
                     V"
BASE COUNT          686 a          765 c          664 g          631 t
ORIGIN      
        1 acacagtttg ctgttccagc caatcccaat ccagatacgc cacgctcaag cccctggccg
       61 tcacaggctg ggacagtgtg agatacccta cgccacgggc cacaatgttc tgtaaaaaat
      121 gcgtgccctg tgagggttcc acgcgctggt cgcgaaaaga tgtctcggca atcaggcgcg
      181 cacccgaaat ctgactccat tccaccggga tccccagact ggggtcggaa gatccccaac
      241 gtcccgggcc gatcagaaaa taacgggccc cctctgtttg caacagcgaa ttcatgcgcc
      301 caatgtgttc tgccacctga cgtgttttgg taccatcgaa ctgatttggt ttcacataca
      361 cgatgtcacg aatatccttg agtaccccat gtcccaaaga aagatcagtg cggcacaaaa
      421 catccttctg gtcaaaaccc accgtttcca cggcaccgtg caccaactgc atggccaagg
      481 gcctcatctg caaaacgtac aaagcaggtc ccgtctcggc gtgaacatcc agcgcaaatt
      541 caacctcgac cgcaaaccct gcctcttgcc ggatgatgtc gagcagttcg gccaacacct
      601 ccgccagggg gatatcacgc cacttgagga tgttgttgaa cgtaatcacc cgcggcccgg
      661 gcagaccaaa attatcgcgg atggtgtggt cctgcacatg atagacgctc cccacttgtt
      721 gcaatgtttt atcttgttca gcatcttcca actgacaccg gctgagtgcc acctcggggg
      781 acaacataaa atcgacctgt tgttttgaaa gatcgagcgc gtaaaattct ttttgtgagt
      841 atttcagata atcttcgggc ttggcatact gcggcaggac ctcgggacaa ctgggggaga
      901 acctcatcac agaaccaccc gacaccacac cataccccag tcccatcgcg atttgagcga
      961 tcccgccttg cgccttttga tgaccaatgg gataataatt gtacgactgc gccacaccag
     1021 agatcagcgg gtagtaacga tgtccgtgag cttgccccac gacctgctgg atcaccaccg
     1081 ccatactctc ttcctcggtc aagtggggag tgtttgcgat gtaggagcgc gcgttttggg
     1141 aatacagaga cgcgtacacg gccttgatcg ccgagcgcag ttccttgaaa cgaacttggt
     1201 gatcggcatg attgttggga atcatgtacg tggcataaat tccggcaaac ggttgtatgt
     1261 gagaatcttc caggatgctg gacgaacgca cggccaatgg ccccggcatg ttgtgcaaca
     1321 caaagcgcag atgttcccag tgttcatcag agagactggc cttgagaaag gctttgatga
     1381 gttcggcatc attagcggca ttctgcacca catcactcag atgattggat tccataaacc
     1441 gttcaaattc gtcggtgcca aaacaaacgg acttgggaat gcgtatgggg agaagcttgc
     1501 ccagtttctt gatacgtgcc ctggcaaaca aggtgttcaa aaaagcgatg ccgcgaccct
     1561 tgccaccaat agaccccctg cccatgcgca caaagagact ctccgggcca gcctgacgtg
     1621 atgaataatc cgtgatgagt ccctgttgtt cgcaatctcg cgactccttg atttttttga
     1681 gcaggaattc cctgatgtcc tcaatggatt taaaatcgga cacactgtat gatcgcagct
     1741 catcacccaa gacaaacaga ctgcgggcac aaaaccagct cgacaaatga tgccgcgtcg
     1801 catggaattc cagcgagacg ggagggatcg tcatgatgat ccgttccata tcgtacaaat
     1861 cctgcgcccg agcgatttct tcgccatccg gcattttgaa aatgaatgtt ccaaacccaa
     1921 gattctgaat caaaaaattc ctcagactcc tcagaatgtg gggagaattt ttatcaacat
     1981 acatgagccc catctcgcgt gcgcgggcct catttctctc gagcgccgat tgcaacaaca
     2041 cctccaggtt ggggttgtcg cgatgcacat acgcggccaa ttcgaaaccc gcctcataat
     2101 ttcttttgcc gtccttggga aaattgacat cgctgatcag ggccagcacg ctgtgcttgt
     2161 atcgttcata gtgccccacg gcctcttcat aactggtggc cagcacgatt ttgggacgcg
     2221 ccctcattcg atagattttg tgcagatcat tcagcccctc tgaaatcagc gaattggatt
     2281 gcgacatgac cacagaatac agcatcgtca aaaatgaaga gtaacggctc acggaatctt
     2341 caacaacaat gatacagcgc acacccgcgc tcttgatgtc gtgatcgata tttcttttgt
     2401 cctcgataag tttgatgatg gtaaggagaa tgcgtgcgtc cccgcgccag acaaagggat
     2461 aatcgatagc ctcccgatca atgccacccg ggaagtgatc cacatcgctc tcattaaaaa
     2521 tcagtaatac gactggcatg tcaggcacaa tggatttgac catacgccca aaggcattca
     2581 cgttggtgtc ttcgagacgc accatggtca tgaccagatc aaaccgtcgc tctttgagta
     2641 gcgtcatggc ctcctgcgtg ctggaaacat gcgtgatccg gggcgcttgg gacaaattca
     2701 gttcattata ttcagcgtac aggcgttctg tgagacgtcc gtcttc
//
LOCUS       DATDUN010000625         2473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_282411, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000625 DATDUN010000000
VERSION     DATDUN010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282411"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2473
                     /locus_tag="VJC18_08775"
     CDS             <1..>2473
                     /locus_tag="VJC18_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLD45516.1"
                     /translation="KIHIPSSVEEILIDKFNKLEPSQQSLLRWLSVNDEALRLEDLQA
                     LCEGQNVQADLLVLIEKDILEKTSREHTYFFRNLLFVDVVYKLIPKIEIPLCHDRLAT
                     ITEDNPQLYVRHLHHLGHGSRPEIAQKALAALGGHYLQEAEYDEAIQTFLKLIKLQHN
                     AINGNTVSGYFSLGRAYFNSRLFDKAITTYQHIKEVLSKNFVAENIENLILAFNCLVE
                     SHLKIANFDQALKEIEAVKPYLKQSRQKLVNQLKFRNYSAYILIRQNNIAEAEISFRE
                     THQIWLNQLSPEEKQKVLNNRLIEVYLIQQKYDLVIHACEENITFLQQTNCRCELAHN
                     YYMLGEVFLKIIATQEKDSTDNLIQKCVENFLSCEKVAREINDFRIMYSAFNGLGNLY
                     NTLKDFNKALDYYKRALNVVRKVNEPVNAAAISYNIGYILGRNIQFRDAFSYMIYAIN
                     TLEGLQGNYSPHREDFLFLSYQSLAEIHAECNEPAKGHKALDKAEAVINQHEFMQYRA
                     FWKEVRRAHLFIIEQNQKEADTYLEKAKTIAKNEDEKTELSKMRELYVQTFASQTTQP
                     TGITIMSTNTQNTSNEDIKKIIEINRFINSEYDTDQLLKIVLNYAIQLSNAESGIVLL
                     LNQEGELVVRATLNTSAADQEKISMSVAKMALEKGEIITSSDALSDERFDSSESIVLN
                     ELKSVLCLPIRSKYKSVGVFYLDNRYRTNAFENCNVDLLNAFCDQVGISLENSKLINE
                     LLGAKQQLQSNLEKTTAELTEVKNILKSECETYQTKYAYQQIIAKSPKMQEIFKLLDK
                     ITETNLSIFIHGESGTGKELVAKALH"
BASE COUNT          762 a          638 c          543 g          530 t
ORIGIN      
        1 aaaatccaca tccccagcag tgtggaggaa atcctgatcg acaagtttaa caaactcgaa
       61 ccctcgcaac agagcctcct acgctggctt tccgtgaacg atgaggccct gcgccttgaa
      121 gacctgcagg cgctgtgtga gggacagaac gtgcaggccg acctgcttgt actcatcgaa
      181 aaagacattc tcgaaaaaac ctcgcgggag cacacctact tttttagaaa cctccttttt
      241 gtagacgtgg tctacaaact cattcccaaa atcgaaatcc cgctctgcca cgaccgattg
      301 gcaacgatca cagaggacaa tcctcagcta tacgtgcggc acctgcatca tctgggtcat
      361 ggttcgcgcc cggaaatcgc acaaaaagcc ctggcggcac tgggagggca ttatctgcaa
      421 gaggcagagt acgatgaggc cattcagact tttctcaagc tcatcaagct ccaacacaac
      481 gccatcaatg gaaatacagt gagcgggtat ttctcactgg ggagggccta ttttaattcg
      541 cgcctgttcg acaaggccat cacaacctac caacacataa aggaggtcct ctccaaaaac
      601 tttgtggccg agaatattga gaacctcata ttggccttca actgtctggt ggagtcccat
      661 ttaaaaattg ccaatttcga tcaggcactc aaagagatcg aagctgtcaa accttatctg
      721 aaacagagcc gccagaagct ggttaatcaa cttaaattta gaaattattc tgcctatatt
      781 ttgataaggc agaataatat tgctgaggcc gaaatatcat tcagggaaac acaccaaata
      841 tggttgaacc aattatctcc cgaggaaaaa caaaaagtat tgaacaatcg tcttatcgaa
      901 gtttatctga tccagcaaaa atatgacttg gtaatccatg cctgtgaaga aaacatcacg
      961 ttcctgcaac aaaccaattg ccgatgtgaa cttgcccata attattatat gttgggggaa
     1021 gtctttctca aaatcatagc cacccaagaa aaggatagta cggataattt gatccagaaa
     1081 tgcgttgaga atttcttgtc gtgtgaaaag gttgcccgcg aaattaatga ttttcgcatc
     1141 atgtacagcg ccttcaacgg cttgggaaat ctgtataaca cattgaagga ctttaacaag
     1201 gccctcgatt attacaaacg tgccttgaac gttgtccgca aggtcaacga acccgtcaac
     1261 gcggcggcca tttcgtacaa cataggttac atactgggaa gaaacataca attcagagac
     1321 gccttttctt acatgatcta cgccatcaat acactggagg gcctgcaggg caactattct
     1381 ccacacaggg aggactttct gttcctgagc tatcaatctc tggccgaaat tcacgcggaa
     1441 tgtaacgaac cagcaaaagg gcacaaggct ctcgataagg ccgaggcggt catcaatcag
     1501 cacgaattca tgcagtacag ggccttttgg aaagaggtca ggagggccca tttgttcatc
     1561 attgagcaaa atcagaagga ggccgatacg tatctggaaa aagccaaaac aatcgcaaag
     1621 aacgaggatg aaaaaacgga gctctccaaa atgcgcgagc tctacgtgca aacatttgcc
     1681 agccaaacaa cccaacccac ggggatcacc attatgagta ccaacacaca aaacacgtca
     1741 aatgaggaca ttaaaaaaat catcgagatc aaccggttca tcaattctga gtacgatact
     1801 gatcaactgc tcaagatcgt gctcaactac gcgatccagc tctccaacgc tgaatccgga
     1861 attgtcttgt tgctgaatca ggaaggggaa ctcgtcgttc gggccaccct caacaccagc
     1921 gcggccgatc aggaaaagat cagcatgagc gtcgccaaga tggcgctgga aaaaggcgag
     1981 atcatcacgt cctccgacgc cctcagcgat gaacgcttcg actcctcgga atcgatcgtg
     2041 ctcaacgaac tcaaatcggt gctgtgtctg cccatccgct ccaaatacaa aagcgtgggc
     2101 gtgttttatt tagacaaccg ctaccgcact aacgcctttg aaaactgcaa cgtggacctg
     2161 ctcaacgcct tctgtgatca ggtgggcatc tcgctggaga acagcaaact catcaatgaa
     2221 ctgctcgggg ccaaacaaca actgcaaagc aaccttgaaa aaaccacagc ggaactcacc
     2281 gaggtcaaga acatcctcaa gagtgaatgc gaaacgtacc agaccaaata cgcctaccag
     2341 caaatcatcg ccaagagtcc caagatgcag gagatcttca agctgctcga caaaatcacc
     2401 gagaccaatc tgtcgatctt catccacggt gaatccggga ccggcaagga actggtggcc
     2461 aaggccctgc att
//
LOCUS       DATDUN010000626         1226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_488711, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000626 DATDUN010000000
VERSION     DATDUN010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488711"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VJC18_08780"
     CDS             complement(<1..1032)
                     /locus_tag="VJC18_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLD45517.1"
                     /translation="MAEKTIARVCLVIFLMVWVSCDYIHISVGKKKENLSVTESPGQS
                     QQEVPVSGVALKESTPATTTPVASQTQPSVPTTLTPEQIFSSYRDSIVEITMMDYLGR
                     PTGTGSGVIISDKGLAVTNHHVLDVAVTAQVRLSNGGMYPVKEVKRVDEANDLVTFVI
                     DASTTPMKPVPLGNSKTLKVGEPVVAIGNPAGLERTLSTGNVSGFRKVSGVDDWVQFT
                     APISHGSSGGAIFDMKGQLVGVVFSSGVDAERTNQNLNFAVPINVLKHMVAGEIVFSN
                     EETLAFLKDERDYLSVNFSLFANLQGYQSIEPVLAYNRVQNYDGYMRELSRFRSQEMV
                     RLGFLNRLQT"
BASE COUNT          307 a          332 c          265 g          322 t
ORIGIN      
        1 tgtctgaagg cgattcagga aacccaaccg aaccatctct tgtgacctaa aacgcgacaa
       61 ttctcgcatg taaccatcat aattctgtac ccggttatag gccaatacgg gctcaatgct
      121 ctgataacct tgcaaattgg caaataacga aaaattcact gagagatagt ccctctcatc
      181 cttgagaaag gccagggttt cttcgttcga aaaaacaatc tcccccgcca ccatgtgttt
      241 gagcacattg atgggaacag caaaattaag attctgattt gttctttcgg cgtcgacacc
      301 agagctaaaa acaacgccaa ccaattgtcc tttcatatcg aatattgccc ctccgctact
      361 gccatgactg atcggtgctg taaactgcac ccagtcatcg acgccactca cttttctgaa
      421 accactcaca ttccccgtgg acaaggttct ttccaaacct gctggattcc caatggcgac
      481 cacgggctca cctaccttga gtgttttgct gtttcccaaa ggaaccggct tcatgggtgt
      541 ggtagaggca tcgatcacga acgtcaccag atcgttggct tcatcaaccc ttttgacctc
      601 tttgacggga tacatccccc cattggacaa tctgacctgc gctgtcacag ccacatccag
      661 cacgtgatgg ttggtcaccg ccaaaccctt atcactgatg atcaccccac tccctgtacc
      721 tgtaggcctg cccagataat ccatcatggt aatctcaaca atgctgtctc tgtaagaact
      781 aaaaatctgt tccggggtca atgtggtggg cacagaaggt tgcgtctgag aagcaaccgg
      841 tgtggtcgtt gcgggagttg actccttgag agccacgcct gacacaggaa cctcttgctg
      901 tgactgtccg ggagattctg tgactgaaag attttctttc ttcttgccaa ccgagatgtg
      961 gatgtaatca caggaaaccc aaaccatcag aaaaataacg agacaaactc tagcaattgt
     1021 tttttccgcc ataacaaact cctcttgtgc aaagctctct catctgtcaa aaaaacgggc
     1081 ttttcaacgc atggcgagtg tacggtaggt cactacgcgg cctgtcatca tttttaccgt
     1141 cactgtcgta tacgcctgcg atggggtctt ggccgtttgt gtttgcgact gaggttgact
     1201 ccgttctctg gcttgagact gtttgg
//
LOCUS       DATDUN010000627         1330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_401237, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000627 DATDUN010000000
VERSION     DATDUN010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401237"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VJC18_08785"
     CDS             complement(<1..450)
                     /locus_tag="VJC18_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HLD45518.1"
                     /translation="MRLPPTITERIAKTILMLATVAAVSALLFVIIFIMVGGFRHLSW
                     DFLTQPILDMGREGGILPAIVGTLMIVFVSVGIGAPLGIASAIYLAEYTREGKVAQSI
                     RFGINCLAGVPSIIFGLFGYILFVVLLGFGWSIVSGGLSLAAMILPTI"
     gene            complement(453..1289)
                     /gene="pstC"
                     /locus_tag="VJC18_08790"
     CDS             complement(453..1289)
                     /gene="pstC"
                     /locus_tag="VJC18_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HLD45519.1"
                     /translation="MDKIISKILFLAACSSTAILALIALFIFKEGVPIMAKYGLGHFL
                     LGREWAPLSGQFGIFTFIVGSLCITFGALVLGVPLGVACAVYIAEFAPKKWALVLKPA
                     IELLAGIPSVVYGFLGVMWLAPFIRNHFGGPGLSILAASCVLAIMTLPTIVGVSIDAL
                     NAVPKNYKQGSLALGATQWQSTRHVILPAARSGILAGVILGMGRAVGETMAVIMISGN
                     TTKIPNSALDSVRTLTANIAIEMGYAVGEHRQALFATGVVLFFFIMILNIVALKVSKK
                     KF"
BASE COUNT          409 a          342 c          312 g          267 t
ORIGIN      
        1 aattgtgggg agaatcatgg ccgcgagtga cagaccccct gacacgatcg accagccaaa
       61 gcccaacaac acgacgaaca agatataacc aaacaaacca aaaataatag aggggactcc
      121 tgccaaacaa ttgatgccaa agcgaatgct ctgggccacc ttgccctcac gcgtgtattc
      181 tgcaagatag atggcgcttg caattccgag cggagcgcca atcccaaccg acacaaagac
      241 aatcatgagc gtccccacaa tggccggcaa aattccacct tctcgtccca tgtctaaaat
      301 aggttgtgtg aggaagtccc aagagaggtg tctgaagcca ccgaccatga tgaaaataat
      361 cacaaacaaa agagcactca cagccgcaac ggtggcaagc atgagaatgg tcttggcgat
      421 cctttcggtg atggtcggag gtagtcgcat agttaaaact tcttttttga gactttcagt
      481 gccacaatat tcaaaatcat aataaagaaa aacaaaacaa cgcctgtggc aaacagagct
      541 tgtctgtgtt cgccaacggc gtaacccatt tcaatggcaa tgttggccgt gagcgttctc
      601 acagaatcca gcgcggagtt tggtattttg gttgtgttgc ccgaaatcat gatcacagcc
      661 atggtttcac ccacagcacg ccccatcccc aaaatcactc ccgctaaaat tccagaacga
      721 gccgccggca gtatcacgtg ccgcgtgctt tgccattgcg ttgctcccaa cgcgagtgaa
      781 ccttgtttgt aatttttggg gacagcgttc agtgcatcaa tcgaaacacc cacgatggtg
      841 ggtagtgtca tgatggcgag tacgcaggaa gctgccaaaa tggaaagtcc aggtcctcca
      901 aaatgattgc gaatgaaggg tgccaaccac atcacaccca gaaaaccgta gaccacggat
      961 ggaatgcctg ccaaaagttc aatcgcgggc ttgagaacca gggcccattt tttaggggca
     1021 aactcggcaa tgtaaactgc acaggccaca cccaacggca cacccaaaac aagggcacca
     1081 aaggtgatgc agagcgagcc aacaataaat gtaaaaatcc caaactgacc ggaaagaggt
     1141 gcccattcac gcccaagcaa aaaatggcca agtccatact tggccatgat gggcacaccc
     1201 tctttgaaga taaagagggc aatcaaagcc aagatggctg ttgaggaaca ggcggcgagg
     1261 aatagaattt tggagatgat tttatccatt ccgattattt tgcagcaatg agaccctctt
     1321 tttccaagat
//
LOCUS       DATDUN010000628         2877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_526304, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000628 DATDUN010000000
VERSION     DATDUN010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526304"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VJC18_08795"
     CDS             complement(<1..433)
                     /locus_tag="VJC18_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45520.1"
                     /translation="MKKIVVLTLLLVLLCPDVFAVEPKVKKNRFFDAIKEYASKDLAL
                     NDVLEFSYFDMGETSLINDTTDEGLSPPTQDLDSENTDEAVAVPPTDVLSSQQEIKKA
                     IDQLPKLFYVSLKNHIERQRVPVNLYSGEGPGYTNPLKLYIK"
     gene            complement(430..1371)
                     /locus_tag="VJC18_08800"
     CDS             complement(430..1371)
                     /locus_tag="VJC18_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLD45521.1"
                     /translation="MDFFAIGLVVFCIILVMKTIKVVPQQKAYVVERLGKYHTTLEAG
                     LRVIVPFIDKIQYKHDLKEVVLDIAEQICITKDNVSVGIDGILFFRVVDPKAASYGVG
                     KFVMAIVQLAQTTLRSEIGKIDLDTTFEERTKINNAVVAAVDHATDAWGVKVLRYEIK
                     SITPPRDVLDAMEKQMRAEREKRAKILESEGDRDSRINRAEGLKQEAIKNSEAEKQKK
                     INEALGQAEAILSVAKATAEGIKNVGEAVSLPGGQYAVQLRVAEEYIKEFGHLAKNTN
                     TMIIPASASDLSGMIATAMSVFDKVKTEDVANKLTRV"
     gene            complement(1395..1826)
                     /locus_tag="VJC18_08805"
     CDS             complement(1395..1826)
                     /locus_tag="VJC18_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HLD45522.1"
                     /translation="MQNISPTIIWAIVGLGLIVLEIFTTSFFILFFGLGALIVAGIKL
                     FGLSNLPIELVIFALIGVSGIIFFRKKVSDSFKNTKGIQIDKEKIITLSEDIAAGKTG
                     SVTYLGSPWRATNKSADDMKKGEDVRIENIDGVTLMLNKKI"
     gene            complement(1968..>2877)
                     /locus_tag="VJC18_08810"
     CDS             complement(1968..>2877)
                     /locus_tag="VJC18_08810"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoate decarboxylase"
                     /protein_id="HLD45523.1"
                     /translation="YYSSQHEYPFLNISQIFHRNGAIFPATVVGRPPQEDHFITNYLQ
                     EMFAPLIRLTMPQVKALWAYEEAGVHTLAGAVVCDRYPREALTTALRILGEGQLSLTK
                     NLFVTDQPCDLKNIKEILVCVLERIDFATDLFVLSHTSQDTLDETGPKPNHGSKSIFL
                     GVGEKKRALPNTFSGPLPSPFSHALVFCPGVLCLTAPTFVTDQNCVTRIGEHACFDAW
                     PLVFIFDDAALACASPESFLWHAFTRFEPAADIGAKRIRVERLHISFTPPMTVDCRCK
                     PWYPKTLESDPQVVRGVQGLIDQYLI"
BASE COUNT          704 a          690 c          719 g          764 t
ORIGIN      
        1 ccttgatgta cagtttgagt ggattggtgt agccgggccc ttcccctgaa taaaggttca
       61 cagggaccct ttgtcgttca atgtgattct taagactcac ataaaaaagc ttgggcaatt
      121 gatcgatcgc ttttttgatc tcttgttgag aacttaatac atccgtggga ggcacagcga
      181 ctgcttcgtc agtattttcg ctatccaggt cctgggttgg tggcgacaaa ccctcgtcgg
      241 ttgtgtcgtt gatgagactg gtttctccca tgtcgaaata ggaaaactcc agcacatcat
      301 tgagggccaa gtcctttgag gcgtattctt tgatggcgtc aaaaaacctg tttttcttca
      361 ccttgggctc aacggcaaag acgtcgggac aaagcagaac gaggagcagt gtgaggacga
      421 cgattttctt catacccgcg tcagcttgtt ggcaacatcc tctgttttga ccttgtcaaa
      481 gacggacatg gcggtggcga tcatgccact caaatcactg gcactggcag gaatgatcat
      541 cgtattggtg tttttggcca gatgaccaaa ctcttttatg tattcttccg ccacgcgcag
      601 ttgcacggcg tattgaccac caggcagaga aaccgcttca ccaacatttt tgattccttc
      661 ggctgttgcc tttgccacag agaggatggc ctcggcctga ccaagggctt cgttgatttt
      721 cttttgtttc tcggcctcgg agtttttgat ggcttcttgt tttagaccct ccgccctgtt
      781 gattctggaa tcgcgatcgc cttcggactc aagtatcttg gctctctttt ctcgctcggc
      841 gcgcatttgt ttttccatgg cgtccagcac atctctgggg ggcgtgatcg atttgatttc
      901 gtagcgcaaa accttgacgc cccatgcatc ggtggcgtga tcgactgcgg cgacaacggc
      961 gttgttgatc ttggttcttt cttcaaacgt ggtgtccaga tcaattttgc caatttcaga
     1021 gcgcagggtt gtttgtgcca attgcacaat ggccatgacg aatttgccta cgccatatga
     1081 tgcggccttg gggtccacga cacggaagaa caggatgccg tctatcccga cggacacgtt
     1141 gtctttggtg atacagattt gttccgcgat gtccaacacg acttctttga ggtcgtgctt
     1201 gtattggatt ttatcgataa agggaacgat caccctcagg cctgcttcca gcgtggtgtg
     1261 atattttccc aagcgttcca ccacgtaggc cttttgttgt ggcacgactt tgatggtttt
     1321 catgaccaag atgatgcaaa acacgaccag gccaattgca aaaaaatcca taaatgtctc
     1381 ctttggttaa tttgttaaat ctttttattc aacatgagtg tgaccccgtc gatgttttcg
     1441 atcctgacgt cctctccctt tttcatgtcg tcagcggact tgttggtggc cctccaagga
     1501 gagcccaaat aggtgactga tcctgttttt ccggcggcaa tgtcttcgga aagcgtgatg
     1561 attttttcct tgtcgatctg aattcccttg gtgttcttga agctgtcaga cacctttttt
     1621 ctaaaaaaga tgatgcccga gacaccaatc agtgcgaaga tgacgagttc gatcggcagg
     1681 ttgctcagac caaacagctt gatgccggca acaatcaagg cccccaaacc aaaaaataaa
     1741 ataaaaaaac ttgtggtgaa aatctcgaga acgataagcc ccagacccac gatggcccat
     1801 atgatggttg gagagatgtt ttgcataacg accccctaat ccggcgttgc cggaaattcg
     1861 aaacctgaat ttttaaatcc gaaacaaagt ccaatctcag attcaaaaaa aacagctcac
     1921 cgtgtcaccc aaaaaagacc gcttcgcagt aggtttgcga ataggttcta aatcagatat
     1981 tgatcaataa gcccctgcac tcctcgcaca acttgagggt cgctttcaag ggttttcgga
     2041 taccaaggct tacagcgaca atccacagtc atgggcggtg tgaacgaaat gtgcagtctt
     2101 tcaactcgga tccgctttgc cccgatatcc gcggcgggtt caaagcgcgt gaacgcgtgc
     2161 cataaaaaag actcaggtga agcacaggca agcgccgcgt catcaaagat aaaaacgagt
     2221 ggccacgcat cgaagcaggc gtgttctccg atgcgtgtca cacaattctg gtcggtaaca
     2281 aaggttggtg cggtcaaaca caacacgccg ggacaaaaaa caagcgcatg tgaaaaaggg
     2341 gatggcaaag ggcctgagaa tgtgtttggc agggcccttt tcttttcacc cacgcccaga
     2401 aaaatggatt ttgatccgtg attgggtttg ggacccgttt cgtcaagagt gtcttgggag
     2461 gtgtgggaga gcacaaaaag atcggtcgca aaatcaattc gttccaacac gcagacaaga
     2521 atctctttga tgttcttgag gtcgcaaggc tgatcggtga caaatagatt ttttgtgagc
     2581 gagagctgtc cttctcccaa aatcctcagc gcggtggtca gggcctcacg tgggtagcgg
     2641 tcacaaacaa cggctccggc cagggtgtgc accccggctt cttcataggc ccataaagct
     2701 ttcacctgag gcatggtcag acgaatcagt ggcgcaaaca tttcctgcaa ataattggtg
     2761 atgaaatgat cctcttgtgg cggcctgccc acgacagtgg ctggaaaaat ggcgccgttg
     2821 cgatgaaaaa tctgggagat gttcagaaaa ggatattcat gctggctgga ataataa
//
LOCUS       DATDUN010000629         1809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_488911, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000629 DATDUN010000000
VERSION     DATDUN010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488911"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..>1809)
                     /locus_tag="VJC18_08815"
     CDS             complement(263..>1809)
                     /locus_tag="VJC18_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLD45524.1"
                     /translation="VVPRCIFLKASPDPPIVHIVSTGYQQIVPPVVNHPRDIADADIT
                     LLSVQALIPLLKSRQITSQTLVEAYIQKIKKVNPALNAVVRDRFDEAREEALRADVLL
                     SQQREQTIPLLTGIPCTIKEAFAVRGMPQTSGLVGRQGQVAEQDAPCVHRLRQAGAIV
                     LGVTNVSELCMWMESANKVYGRTNNPHDTRRIAGGSSGGEGAIIGAGASLFGLGSDVG
                     GSIRMPAFFNGVFGHKPSSCIVPNEGQFPGTVGRASHYLTTGILCRYAKDLMPLMSIL
                     ATPAPDENKKQLLQWPDSSPVDFAKITVVDIPGNGMNKVAPDVRQSQARCAEHFRSLG
                     AKVVTREFKLLKKSFSFWAAHMSLADEIPFGTLMGQGQRISVAKELARWLVNQSDHTF
                     PAIMLALLEQMPVSSKQKKNYVDQSLELKNQVEEAMGPQGVLLYPTFPTVAPRHNAPI
                     FKLFPWVYTAIFNALEFPATSVPLGLNKNNLPLGLQVVSRWGADHLTIACACELEKRF
                     GGWSIG"
BASE COUNT          460 a          477 c          446 g          426 t
ORIGIN      
        1 cagatttgac tgtgaaaaag attgttcggt tcaagccttg gcagaatatg aaggcagtaa
       61 actcaactct aacgagtcca gttcacattt ttacaggaag ggactagtaa ggtttttggg
      121 tatcaggccc ggatccttga aaaacaagaa tcagagcgtg gcgcactcat agcacagatt
      181 tttttgattc acaagagata aagacagatt acaaaaataa cgaatcataa gatagcacta
      241 ggagtcaatc tgaagacagt gctcaaccaa tgctccatcc accaaatcgt ttttccaact
      301 cacaggcaca agcgatcgtg aggtgatccg caccccagcg tgacacaact tgcaacccca
      361 aggggaggtt atttttattc aatcccaacg gaacacttgt tgccggaaat tccaatgcat
      421 tgaagatggc tgtgtacacc cagggaaata atttgaagat gggcgcattg tgtctggggg
      481 caacggtggg aaaggtgggg tacaacaaga ccccctgagg acccatggcc tcttccactt
      541 gatttttgag ttcaagactt tggtcaacgt aatttttttt ttgtttcgat gaaacaggca
      601 tttgttccaa cagggccagc atgatggcgg gaaaagtgtg atctgattga ttcaccaacc
      661 acctggcaag ttctttggcc acactgatgc gctggccttg tcccatgagt gtgccaaaag
      721 ggatctcgtc cgccagactc atgtgcgcgg cccagaacga aaaagatttt ttaagcaatt
      781 tgaattctcg cgtaacgact ttggccccca gacttctaaa atgctcagca cagcgcgcct
      841 gcgactgacg cacatctggc gcaactttat tcattccatt acccgggatg tcgaccacag
      901 tgattttggc aaaatcaaca ggcgatgagt ctggccactg aagcagctgt ttcttgtttt
      961 cgtcaggggc gggtgtggcc agaatactca tgagtggcat gaggtccttg gcatagcgac
     1021 acaggatgcc cgtagtgaga taatgagagg ctcgcccgac agtccccgga aactgccctt
     1081 cattaggcac gatgcaggat gacggtttgt ggccaaacac accattgaaa aaggccggca
     1141 tcctgatgga cccccccaca tctgatccca gcccgaacaa cgaggcccct gccccaatga
     1201 tggcgccctc gcctcccgaa ctgcctcccg cgatgcgtct tgtgtcgtgt ggattgttgg
     1261 tcctgccata caccttgttc gcggattcca tccacatgca cagctcgctc acattggtca
     1321 cacccaatac aatcgcacca gcctgacgca gtctgtggac acaaggggca tcctgctcgg
     1381 caacctggcc ctgtcgtccc accagccccg atgtttgcgg catcccccgc acagcgaagg
     1441 cctccttgat cgtgcagggg attcctgtga gcaaaggaat tgtctgctcc ctctgttgcg
     1501 acaacagcac atcggcccga agagcttcct ctctcgcttc atcgaaacga tccctcacca
     1561 cagcgttcaa cgcgggattt acctttttga ttttttgaat gtaggcctct acgagggtct
     1621 gcgaagtgat ttgccgtgat ttgagtaacg gaatcaatgc ctgcacagag agcaacgtga
     1681 tgtcagcatc ggcaatatct cttggatggt tcaccactgg ggggacaatc tgttgataac
     1741 cagtcgacac gatgtggaca ataggaggat ctgggctggc tttcaagaaa atacaccgcg
     1801 gcacaaccc
//
LOCUS       DATDUN010000630         1642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_283179, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000630 DATDUN010000000
VERSION     DATDUN010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283179"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VJC18_08820"
     CDS             <1..861
                     /locus_tag="VJC18_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD45525.1"
                     /translation="ATKYNKKYSDAHYNQGLVYTEWYRKDHNKEHLGKAVEAFRTATE
                     INSDHPYAHYKLANVYQILGKYEDAILRYRLALEIDPKLEDAWNNLAQLYTVTGQTLK
                     AQQALNHALAANPDSPEAHINMGLNFLKDDNISMAIKEFNEAIKQDPTNELAYFNVGF
                     AYYQQAINLQKQGRSSDVASLLQESIRAYTSAFKIKPNYPQATYNIGYDYQLLKDYPN
                     AVTWFQKTISIDADYHQAYFSLGLVYQLLGQSDNAAKAFCQFVKRNPSGAETDVNTAS
                     DMLKSLGGCH"
     gene            909..>1642
                     /locus_tag="VJC18_08825"
     CDS             909..>1642
                     /locus_tag="VJC18_08825"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLD45526.1"
                     /translation="MLKICVTNRTTGQKQRYKFVQHAVILGRQPNCDVVLEGTGVSRR
                     HTKVTLNRDLVEIEDLGSGNGTVVNHQKIPAHQKVPVKKTDRIRVEEFEIELEAEVST
                     NPLDKPANPDVTDPDIIEIKMIKKVLGALDQDKMPSVVVVDDEFAGKKAYFEEGLDEL
                     VVGRDENCTLSMDSHVLSRRHAIITVKWGQFVVVDQNSKNGTFVNGRRVEEKSIKDGD
                     EIVFGTIKAVFKNPHEFNMDAITESL"
BASE COUNT          456 a          350 c          438 g          398 t
ORIGIN      
        1 gctacgaaat acaataaaaa atattctgac gcccattaca atcagggact ggtgtacaca
       61 gaatggtacc gaaaggatca caacaaagaa catttgggga aggctgtgga ggcctttcgc
      121 acggcgaccg agatcaacag cgatcacccg tatgcccatt acaaattggc caacgtttat
      181 cagatattgg gcaagtacga agatgcgatt ttgcgttatc gattggcgct agagatcgat
      241 cccaagcttg aggatgcctg gaacaatctg gcacagttgt acacggtgac tggacagaca
      301 ctcaaggcgc aacaggctct gaatcacgct ttggcggcca atccggattc tcccgaggcg
      361 cacatcaaca tggggctcaa ctttttgaaa gacgacaaca tcagcatggc catcaaggaa
      421 ttcaatgaag cgatcaagca ggatcccacg aacgaattgg cgtatttcaa tgtggggttt
      481 gcctattacc aacaagcgat caatttgcaa aaacagggac gctcgtctga tgtggcttcg
      541 ttgttgcagg aatccattcg ggcgtacact tcagcgttca agatcaaacc gaattatccg
      601 caagcaacct acaacattgg ttatgattat cagctgctca aggattatcc caacgccgtg
      661 acctggtttc aaaaaacaat ttccattgac gctgattatc atcaggccta tttttcgctg
      721 ggcttggtgt atcagttgtt gggacagtca gataacgccg cgaaggcttt ttgccagttt
      781 gtaaaacgaa acccctcggg ggcggagacc gatgtcaata cagcgagcga catgctcaag
      841 tctttggggg ggtgtcattg attgcatcat tgcttcattg attgattgct tcattcattt
      901 gtttatttat gcttaaaatt tgtgtgacaa atcgaacgac gggtcaaaaa cagcgctaca
      961 aattcgtgca acatgccgtc attttgggac gacagccgaa ctgtgacgtg gtgcttgagg
     1021 gtacgggtgt ttcccgtcgc cataccaagg tgacgctgaa ccgcgacttg gtcgaaatag
     1081 aggatttggg gagcggtaat gggactgtgg tcaatcacca gaagattccc gcgcaccaga
     1141 aggttcctgt caaaaaaacg gatcgtattc gagttgagga gtttgagatt gaattggaag
     1201 cggaggtttc gacgaatccg ttggacaaac cagccaaccc cgatgtgacg gatcccgata
     1261 tcatcgaaat caagatgatc aaaaaagtgt tgggggcatt ggaccaagat aagatgccat
     1321 cagtggtggt ggttgatgac gagttcgcgg gcaagaaggc ctattttgag gaagggctgg
     1381 atgaattggt tgtgggacgc gatgagaact gtacgctgag tatggattcg catgtgctca
     1441 gccgccggca cgccatcatc accgtgaagt ggggccagtt tgtcgtggtc gatcagaata
     1501 gcaaaaacgg gaccttcgtt aacgggcggc gtgtggaaga aaaatcaata aaagatggtg
     1561 atgaaatagt ttttggtacg atcaaggctg tgttcaagaa tcctcatgag ttcaacatgg
     1621 atgccattac tgaatctttg ca
//
LOCUS       DATDUN010000631         6061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_234488, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000631 DATDUN010000000
VERSION     DATDUN010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234488"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VJC18_08830"
     CDS             <1..489
                     /locus_tag="VJC18_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HLD45527.1"
                     /translation="RDRDFLIAECDKHHLEVKDKQNVDLGTVQVILFEELVESQLISP
                     TFITHYPTSVSPLSRRNDADPTVTDRFEIFINGKEIANAFSELNDPVDQKERFLKQVA
                     QRTTGDDEAMFMDEDYVTALEYGMPPTAGEGIGIDRLVMILTNQPSIRDVILFPVLRA
                     GA"
     gene            complement(610..1674)
                     /locus_tag="VJC18_08835"
     CDS             complement(610..1674)
                     /locus_tag="VJC18_08835"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HLD45528.1"
                     /translation="MHKQTLAIICGGVSGEHQVSLLSAQSVLKALDRNRFNPVIIGVD
                     KCGVWRRYDEGNWLNFANDPKRIALANNGSEFPLNPFPGSFQKMGIDAVFPLIHGSFG
                     EDGTLQGLLELVAVPYVGPDVAGSSMAIDKDLTKRILSHAGVNVVPFETFEPQDIKEL
                     DVNTLWSRFSEGCFVKPARCGSSLGIRQVKTKETLKPAIEFALRYDDKVLVEQKIVGR
                     ELECAVLGGTPPLASVIGEIVPKDGFYSYEAKYIDDDGAQLIAPAEVTSAVRDALQKT
                     AIKTFQMLCLEGMARVDFFLANDGVLYVNEVNTIPGFTKISMYPRLLGLSGVPYTELI
                     TKLVDLGIGRFERRKQLVRE"
     gene            complement(1791..2972)
                     /locus_tag="VJC18_08840"
     CDS             complement(1791..2972)
                     /locus_tag="VJC18_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HLD45529.1"
                     /translation="MNSIKSKKILPITLLLLSLLGIFIAAYLTYIHYMVTGGDANFAS
                     FCNVNAKVNCDAVAASAWSTLFGVPVSIWGMLFYVFFWGLNADRLAKNIIRNNTVYQL
                     LLATAGIGFTAFLIAISKIILDTWCLLCAALWIINLFIFILAFKSNTHPFKSYPKILW
                     QDLLWFFKKWYRLVLILLVLSTFVATLFYTAHHERQIKQTQLDQPPIFNELISPISLT
                     GPAHGADSPRLTIVEFSDFQCPHCKKLHDVLSKITRENKKIRVIHKDFPLDHHCHPLI
                     TKPFHQNACQASVFVRCAGQQGKYWEAFDQTFELSRELEDATTFDTLTSLLELNKDQL
                     TACMSDPKITEGIKQDLQEGIDNGIDATPVVIFNGTDQLLSGWSEGKLEKAIQFWMKK
                     K"
     gene            complement(3059..3643)
                     /locus_tag="VJC18_08845"
     CDS             complement(3059..3643)
                     /locus_tag="VJC18_08845"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HLD45530.1"
                     /translation="MSKLYFRYSAMGAGKSLDLLKVAYNYEERGMRVALYNSQLDTRY
                     ETGSIASRTGISKQAIPFSTQTNFYDHFLESFFNKDIKISCILIDEAQFLKKEQVSQI
                     RRLVNKFDIPVICYGLRTDFQAELFEGSLWLMAWADEIEELKTICWCGRKATMNARVV
                     NGKVVKVGEQIMIGGNESYIALCQIHYHKGDIGK"
     gene            complement(4192..4683)
                     /locus_tag="VJC18_08850"
     CDS             complement(4192..4683)
                     /locus_tag="VJC18_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HLD45531.1"
                     /translation="MSVQKVKYKNVILFFCQQLGGELRGKKKLAKLLYFLDFDFYEWH
                     DVSITNDVYKALPMGPYPSHLSDMISEMTDEKLLFAKTEKVHAGYLATEIYTNKFEPD
                     LKVFSKDEIKMLERVVSHYGHLNGKQLEDLSHAEAPYVGTEPSKEIAYELSYYRGTDF
                     ADL"
     gene            complement(4761..5183)
                     /locus_tag="VJC18_08855"
     CDS             complement(4761..5183)
                     /locus_tag="VJC18_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HLD45532.1"
                     /translation="MQNLQNEQISFDYMSAFEILHPSLIMKCRKLFENGHFSESAEKG
                     FKVVRDRLRDLTGFENGSDAFGRGRLQVLGATAKNVESDFHDAVQFLTMSIDKFRNEK
                     AHTANAKITDPKRAYAYLQLSNLAMLLLDNATITNVSS"
     gene            complement(5195..5686)
                     /locus_tag="VJC18_08860"
     CDS             complement(5195..5686)
                     /locus_tag="VJC18_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HLD45533.1"
                     /translation="MATIDGRISTEMYTKPSNKNTFVVVTPSFPSLTEKQIATLIEKK
                     MIEKGFSKASSKKTADIGVFYSYSIGNGSNQILSTPDFVVGGHAIYSETVYPRFFQIV
                     IIDLAKTRLPNKIAIIWQGELRSKGSSDNIIDVAQYFIGALFSSYGKTVTNKGFLRIA
                     PPF"
     gene            complement(5749..>6061)
                     /locus_tag="VJC18_08865"
     CDS             complement(5749..>6061)
                     /locus_tag="VJC18_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45534.1"
                     /translation="TLVLGQDGQDALNEGILKFREMGITIAGNVLRAYNATQLGTIHP
                     DVWRLGLDYFADTFEIIYETNSRQLSSARYMRQLALDYLKRRFPDAEITDRDVPYKYR
                     G"
BASE COUNT         1652 a         1349 c         1426 g         1634 t
ORIGIN      
        1 cgtgatcgcg attttctgat cgccgaatgt gacaaacatc accttgaggt caaagataaa
       61 cagaatgtgg atttgggtac ggtgcaggtg attttgtttg aggaattggt cgagagtcaa
      121 ctcatttcgc ccacattcat cacgcattac ccgaccagtg tgtctccgtt aagccgacgc
      181 aatgatgcag atcccacggt tacggaccgt tttgaaatat ttatcaatgg caaggaaatt
      241 gccaacgcct tttcggaact caacgatcct gtagatcaga aggaacgctt tctcaaacag
      301 gtggctcaaa gaaccactgg ggacgacgaa gccatgttca tggacgagga ctatgtgact
      361 gcgctcgaat atggcatgcc gccgactgcg ggtgaaggga ttgggatcga ccgcttggtc
      421 atgatcctga ccaatcaacc ttccattcgc gatgtgatac tgtttccggt gctcagggca
      481 ggcgcatagg gagacgaaag gacgagggac gaaaggacga gggacgaaag gacgagggac
      541 gtcattgcga gtgaccaacg ggaacgaagc aatccacgat ccacgatgag tcacaaccgc
      601 tagatagttc tattctctca cgagctgttt tcttcgttca aaacgaccaa tccccaggtc
      661 gacgagtttt gtgatcagtt ctgtgtaagg tacgccggag agtcccaaca aacgcgggta
      721 cattgagatt tttgtaaacc caggaatcgt gttgacctcg ttcacgtaaa gcacaccgtc
      781 gttggccaga aaaaaatcca cacgggccat tccctccaaa cacaacatct gaaaggtttt
      841 gatggcggtc ttttgcaggg cgtcgcgaac ggccgaggtc acttcggcag gggcaatgag
      901 ttgggcgcca tcatcgtcga tgtattttgc ctcgtaagaa taaaaaccat ctttgggcac
      961 gatctcgcca atgactgaag ccaacggcgg tgttccacct aatacggcac attccagctc
     1021 gcgccccaca attttttgtt ccaccagcac tttgtcatcg taacgcagtg caaattcaat
     1081 agcgggtttg agtgtctctt ttgtcttgac ctgcctgatg cccagagaag agccacaacg
     1141 ggcgggtttg acaaaacagc cctcagaaaa acgggaccac agagtgttca cgtcgagctc
     1201 tttgatgtct tgcggttcaa aggtttcaaa ggggaccacg ttcacacccg catgactcag
     1261 aattctcttg gtgaggtctt tgtcgatggc catggatgaa cccgccacat cgggacccac
     1321 atagggcacg gccacgagct ccagaagtcc ctgcaaggtg ccgtcttcac caaatgatcc
     1381 gtggatgagt ggaaaaaccg catcaatccc cattttctga aaactgccgg ggaaggggtt
     1441 gaggggaaat tcactgccgt tattggcaag ggcgattcgt tttgggtcat tggcaaagtt
     1501 gagccagttg ccctcgtcgt aacgacgcca cacgccacac ttatcgacgc caatgatgac
     1561 gggattaaaa cggttgcggt cgagggcctt taagactgat tgtgcggaga ggagcgaaac
     1621 ctgatgttcg cccgacacgc cgccacagat gatggctagg gtttgtttgt gcatatcaag
     1681 ttcctgattc taaaaaatcg tttgaatact gaatagcgaa caaagaacaa ggaatgtcga
     1741 aatctgtaca tcgacattcg atattccttg ctcgatattc gatattccca tcattttttt
     1801 ttcatccaaa attgtatcgc cttttccaac ttgccttcac tccagccgga gaggagttgg
     1861 tcggtaccgt taaagatcac gacaggcgtg gcatcaatgc cgttgtcgat cccttcttgc
     1921 aaatcctgtt tgatcccttc cgtgattttg gggtcactca tacaagcagt cagttgatcc
     1981 ttgttaagct ccaaaagcga agtgagagta tcgaaagtgg tggcgtcttc gagttcgcgc
     2041 gacagttcaa aggtctgatc aaaggcctcc caatactttc cttgttgacc ggcacaacgc
     2101 acaaagactg aggcttggca ggcattttgg tgaaagggtt tggtaatcag cgggtgacaa
     2161 tgatgatcga gcgggaagtc cttgtgaatg accctgattt ttttgttttc ccgtgtgatc
     2221 ttggacaaga catcatgcag ttttttgcag tggggacatt gaaaatccga aaattccacg
     2281 atcgtcaggc ggggggaatc agcgccgtga gcaggtccag tcaaggagat cggcgaaatg
     2341 agctcattga aaatgggcgg ttggtccagt tgtgtttgtt tgatctggcg ttcgtgatgc
     2401 gcggtgtaaa acagtgtggc aacaaaagtt gataggacca gcaagatcaa aaccagacgg
     2461 taccattttt tgaaaaacca caacaaatcc tgccagagaa tcttggggta ggacttgaag
     2521 gggtgtgtgt tggatttgaa ggccaaaatg aaaatgaaca gattgatgat ccaaagcgcg
     2581 gcgcacagaa gacaccacgt gtctaaaatg atctttgaga tggcaatcaa aaacgcggta
     2641 aaaccaatac ccgcagtggc cagcaaaagc tgataaacag tattgttgcg gataatgttc
     2701 ttggcgagtc tatcggcatt gagtccccaa aaaaacacat agaacagcat gccccaaatc
     2761 gagacgggca cgccaaagag tgtggaccag gcactggcgg ccacggcgtc acagttcacc
     2821 ttggcattga cgttgcaaaa gctggcaaaa ttggcatcac cgccggtaac catgtagtgg
     2881 atgtaggtca gataggcggc gataaaaata ccgagcaggg agagtagaag caatgtaatg
     2941 gggaggattt tttttgactt tatggaattc atggaactgg tcattgcagt gcacccaaaa
     3001 tgttgaatca atctggtcga aaggtcaccc cgaggcaccg ctcgcgatga caatctcatt
     3061 atttcccaat atcgcctttg tgatagtgta tttgacacag ggcgatgtag gattcgttgc
     3121 cgccgatcat gatctgttcg cccaccttga ccactttgcc attcaccacc cgggcgttca
     3181 tggtggcctt tctgccgcac cagcagatgg ttttgagttc ttcaatttca tcggcccaag
     3241 ccatgagcca cagactgccc tcaaaaagct ccgcctgaaa atcggtgcgg aggccataac
     3301 agatgacagg gatatcgaat ttgttcacca acctcctgat ttgtgacacc tgttcttttt
     3361 tgaggaactg ggcttcatcg atcaggatgc aactgatttt gatatccttg ttgaaaaaac
     3421 tctccaaaaa atgatcataa aaatttgttt gggtagagaa ggggatggcc tgttttgaaa
     3481 tgcctgtgcg ggaggcaatg ctccctgttt cgtaacgggt gtcgagttgc gagttataca
     3541 gggccacacg catgccgcgt tcttcgtagt tgtaggcgac tttcaaaaga tccaaagatt
     3601 ttccggcgcc catggcggag taacgaaaat aaagtttgga cacgatgccc tcctcgaacg
     3661 tgaatagaac ttttttgggt cctgctccgg ggtactattt tttgacgctc ttcgcaagcg
     3721 aagcttgctt cgcaggccgc gtcaaaataa tagtacaccc gtatcaccca aaaaaacaaa
     3781 ggtcgtgtat cgtggatcgt ggagcgtgta aagaaaatcc ctagaaaaaa tcgctgatac
     3841 gtttaatggc ttcattcttg atttcgttgt cgctgtagtt atcaatatgg gaataaatgc
     3901 aaagaaatac aatatgagag ccctttacgg caaaccataa tcgaggaaat tggtttggtc
     3961 gcaagccgga gcgaggcaaa accaattcaa ctttccacaa cgcccacaaa tcggtatgct
     4021 ggattcgatg caattttccg ggactgatta cttgttcagg ctgaagagga ttaaattgtc
     4081 tatcacacag tctttcaaaa gtcttaaggg cctcattgac agttttgtgc ttgcgttcaa
     4141 gtgatttgat ttcacggaga atggaatcat gataataaaa ttgcaatccg ttcataggtc
     4201 ggcaaaatca gtcccgcggt aataggaaag ttcgtaggca atttctttgg aaggttctgt
     4261 cccaacataa ggggcttcgg catgcgaaag atcttcgagt tgcttgccgt tgagatggcc
     4321 atagtggctg acaacacgct caagcatttt gatctcatct ttggaaaaca ctttcaggtc
     4381 aggttcaaat ttgtttgtat aaatttctgt tgccaggtac ccggcgtgca ctttttcagt
     4441 tttcgcgaag agtagttttt catcagtcat ttcggaaatc atgtctgaca aatgtgaagg
     4501 gtagggtccc atcggcaatg ccttgtaaac atcgtttgtg atggaaacgt catgccattc
     4561 atagaaatca aagtctaaaa aatataacag ctttgccagt ttctttttgc cacgtaactc
     4621 cccgcccagt tgctgacaaa aaaacagaat gacattttta tattttactt tttgcacaga
     4681 catacaaaca gatccctagt tcattgtatt ttcaaagttc tataatagtc aattgacttg
     4741 ttggaaaatc gcccagcgac tcaagatgac acatttgtta ttgtggcgtt atcaagtaat
     4801 agcatcgcca aattgcttaa ttgcaaatat gcgtaagccc gctttggatc tgtgattttc
     4861 gcatttgcag tatgtgcttt ttcattcctg aacttgtcta ttgacattgt aagaaattgc
     4921 acagcgtcat gaaaatcgct ttcaacattt ttcgcagttg caccaaggac ttggagtctg
     4981 cctctaccaa aagcgtccga gccattctca aaaccagtta aatcccgtag acgatctctt
     5041 acaaccttaa aacctttctc agcagattca gaaaaatggc cattttcgaa caatttcctg
     5101 catttcatta tcagactagg gtgtaaaatc tcgaaagcag acatataatc aaacgatatt
     5161 tgttcatttt gtaggttttg cattatttgg agaatcaaaa aggcggtgct atacgcagaa
     5221 atcctttatt tgtaacagtt ttgccgtaac tactaaataa ggcaccaatg aaatactgag
     5281 caacatcaat tatgttatct gatgatccct tactacgtaa ttctccttgc caaattatgg
     5341 caatcttgtt tggcaatctt gttttcgcga gatcaataat aacaatctgg aaaaaacgcg
     5401 gatacactgt ttctgagtag atagcgtgtc ccccaacaac aaaatcaggt gtgctcagta
     5461 tctgattgct cccattgcca attgaatatg aatagaatac cccaatatct gctgtttttt
     5521 tcgaactagc ctttgaaaaa cctttctcga tcatcttctt ttcaattaat gtggcaattt
     5581 gtttttctgt taaacttggg aaggatggag tgactactac aaaagtattt ttattggacg
     5641 gtttagtata catttctgta ctaatcctac catctatagt tgccacacaa ctactcatca
     5701 caattgcaaa tatccagata actgataata aagcatgtcg acttgtcatt atccccggta
     5761 tttataaggc acgtctcgat cagtgatttc cgcgtctgga aaacgtcgtt ttaggtagtc
     5821 caatgccaac tgccgcatgt atcgggcaga tgataattgt cggctattgg tttcatatat
     5881 tatttcgaaa gtgtccgcga aataatccaa gccaagtctc cagacatcgg ggtgaatggt
     5941 gcccagttgt gtggcattgt aggccctgag cacatttcca gctatggtga tgcccatttc
     6001 tctgaatttg agaatgcctt cgttcagggc atcctgaccg tcttgaccaa gcaccagggt
     6061 t
//
LOCUS       DATDUN010000632         1039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_611630, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000632 DATDUN010000000
VERSION     DATDUN010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611630"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..>1039
                     /locus_tag="VJC18_08870"
     CDS             137..>1039
                     /locus_tag="VJC18_08870"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HLD45535.1"
                     /translation="MPNNKTSPTFKVTSLGCPKNLVDSEIMIGLMGQSGFRVVPTDQP
                     AQVHIINTCSFVGDAKSESIDHILSASQTKKEGGISLLVVTGCLPQRYQKKISRLLPE
                     VDLFVGTGDYERLPTLIAQKLIGTKKRNFIHQQQFLPTHLTPRVQSTAFYTKYVKISE
                     GCSHQCSFCIIPTIRGKLLSRQPEDIVQEIKTGVASGIREFNLVAQDLNEYGRDLTPK
                     SSLNQLLKLTSDIKGDYWIRLLYMYPLQFPDRLIDTIADHPHVARYVDIPLQHISDHL
                     LKKMRRGSSRRYIMRLIDQLKNKIP"
BASE COUNT          291 a          299 c          224 g          225 t
ORIGIN      
        1 tcgcccctac acattgattg atgtttgcaa aaaatagcgc gccgtggcag catgcccgcc
       61 gacctgtcca ccgaagctca cagagcgtag gtggaagctt tagcgcaggc agggacccca
      121 aaggcaagac actcccatgc caaacaacaa aacatccccc accttcaaag tcacctccct
      181 cgggtgccca aaaaatctgg tcgacagcga aatcatgatc ggactgatgg gacagtccgg
      241 cttccgtgtg gttcccaccg atcagccagc tcaggtccac atcatcaaca cttgttcgtt
      301 tgtgggcgat gccaaatcag aatccatcga ccacattttg tcggcctcgc aaacaaaaaa
      361 agagggtggg atctctcttt tagtggtcac gggatgcctg ccgcaacgct accagaaaaa
      421 aatttcgcgc ctgttgcccg aagtggatct ttttgtggga acaggtgatt acgaaagact
      481 ccccacactc atcgcgcaaa aactcattgg cacaaaaaaa agaaatttta ttcatcaaca
      541 acaatttctc cccacgcacc tgacgcctcg tgtgcagagc accgcctttt acacaaaata
      601 cgtaaaaata tctgaagggt gttcgcacca atgcagtttt tgcattatcc ccaccatccg
      661 gggaaaactt ctctcgcgac agcccgagga catcgtgcag gaaatcaaaa cgggggttgc
      721 gtcaggcatc agggaattca atctggtggc acaggatctc aacgagtatg gccgtgacct
      781 gacgcccaaa tcttcactca atcaactcct caaattgacg tctgatatta agggcgacta
      841 ttggatccgt ctgttgtaca tgtaccctct gcagtttcca gaccgactca tcgacaccat
      901 cgccgatcac cctcatgtgg cgcgttacgt agacattccc ctgcaacaca tcagtgacca
      961 cttgctcaaa aaaatgaggc gtgggtcatc acgacgctac atcatgcgat tgatcgatca
     1021 actcaaaaac aaaattccg
//
LOCUS       DATDUN010000633         1106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_73951, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000633 DATDUN010000000
VERSION     DATDUN010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73951"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VJC18_08875"
     CDS             complement(<1..360)
                     /locus_tag="VJC18_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45536.1"
                     /translation="MCVALTTLKDITDTDEAFCTQMASVLARFTNHPNSRFLLSDSID
                     ALQGLRIDEREKLIEEMFGYYRQILADIGVTLGLMELLDEPSDVATSRLREVRSNIHG
                     LLFLGLNSLAGRLAYVNW"
     gene            complement(396..1025)
                     /locus_tag="VJC18_08880"
     CDS             complement(396..1025)
                     /locus_tag="VJC18_08880"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HLD45537.1"
                     /translation="MKLNQIIIEFVFNTPARVCAEKLGIHRNTVNFWYQRMRKGIAQL
                     PDPEPFSGEIEIDESYFGKKKTGKGRPGTVQGQVAVFGLRERKSGLVVAEVVSGTSAK
                     ELIPLIQKHVVKGSTIYSDGHGAYCHLSKLGYRHHVVYHEHTFVDQKVIHTNSIESFW
                     AYAKHTLRPLKGLPYEAYPEHIKMIQKRYNTFANKKLRLTLRRILQNSY"
BASE COUNT          260 a          265 c          256 g          325 t
ORIGIN      
        1 ccaattaaca tatgccagtc ggcctgccaa ggaattcaaa cccaggaaga gcaaaccatg
       61 aatgttgctc cgcacttctc gcagacgaga agtggcaaca tccgatggct catccaaaag
      121 ctccatgagg cccaatgtga ccccaatgtc ggccaggatc tgccggtaat agccgaacat
      181 ctcttcgatc agtttctcac gctcatcgat gcgcagtccc tgcagtgcat cgattgaatc
      241 tgacagcaga aatcgactat ttggatggtt tgtgaatctt gccagcacac tggccatttg
      301 cgtacagaac gcctcatcgg tgtcggtaat gtctttcaaa gtggtcaggg cgacacacat
      361 ttcatccctg ttattcttgg ggtctggcac agatgttaat agctattttg tagaattctt
      421 cgcaaagtta agcgaagttt tttgtttgca aaagtgttgt accttttttg gatcattttg
      481 atatgttccg gataggcttc gtaaggcaat cctttaagtg gtcgaagtgt gtgtttggca
      541 tacgcccaga agctttcaat ggagtttgtg tggatgacct tttggtcgac gaaggtgtgc
      601 tcgtgataca cgacatggtg acggtaaccg agctttgaga gatgacagta cgctccgtga
      661 ccatctgagt agatcgttga cccttttacg acatgtttct ggatgagcgg aatgagttcc
      721 ttcgcgctcg ttcctgagac tacttccgcg acgacaagtc cactttttcg ctcacgtaaa
      781 ccaaaaacgg ccacctgtcc ttggacggtg cccggtcttc cttttcctgt tttcttcttt
      841 ccaaaataac tttcgtcgat ctcgatctca ccggaaaatg gctcagggtc tggtaattgt
      901 gcaatccctt ttctcattct ttgataccag aagttcaccg tgtttcggtg aatcccgagc
      961 ttctcagcac acactcgagc tggagtattg aaaacaaatt caatgatgat ttggttgagt
     1021 ttcatgggag tcagtcgagt ccctttgatg tgtttgtaca tccccaaact ctaccctgat
     1081 tttgatgaaa gatctgtgcc agaccc
//
LOCUS       DATDUN010000634         2936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_34346, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000634 DATDUN010000000
VERSION     DATDUN010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34346"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VJC18_08885"
     CDS             <1..583
                     /locus_tag="VJC18_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HLD45538.1"
                     /translation="SADDAIEAAEQAGFPVAMKIVSPDIQHKSDFGGVKLGLNNREEV
                     RDAYDLMMLRITRRVPEAVITGVYVEKMCPKGREVILGMSRDPQFGPMLMFGLGGIFV
                     EVMKDVTFYLAPITEEESMQMLRTTKSYQLLKGARGQAAVDLHAVAVSLQKISQLVTD
                     FPMIQELDINPFMVAAVGQASFVADARMTLCRG"
     gene            728..2602
                     /locus_tag="VJC18_08890"
     CDS             728..2602
                     /locus_tag="VJC18_08890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLD45539.1"
                     /translation="MNNHSWKEKYKNVIMTADAAVKHIHPGQRVFVGTGCAQPQQLVK
                     ALVSRADELVDTEIVHLLTIGQAPYAHKELAQKFQVNSFFISDNVRDVIQEGLGDYTP
                     IFLSDIPELFKSGQMPLDVALIQVSPPDEKGNCSLGVSVDIVKSAAQNARVVIAQVNE
                     QMPRTLGDSFLHVHDFDYLVPVDVPLIEVTWGGLTEETTQIGSYVASLIDDGATVELG
                     IGRVPHAVLLSLKNKKDLGIHTEMFSDAIIDMIESGAVNGSKKSMDKGKVTASFCLGT
                     KRLYDYIHNNPAFAFHPTEHVNDPHVISQQYKMVAVNVALEVDLTGQVCADSLGTKFY
                     SGIGGQVDFNRGAARCKDGKAIIAMESTALDGKVSRIVSRLTPGAGVVTSRGDVHYVV
                     TEYGVAYLHGKNIQQRALALISIAHPDYREQLLKEAIAYKYIRADLASVGDRIHVGPK
                     EMRTTQVLDDGTQVFYRPIHPTDEPKIKELMYSLSQKSLYTRFMSRLRWVSQKQVREF
                     IYVDHRNEVEIVACLPEASGDCVLAIGGYYLDPKTNRAEVAFIVRDEWQNRHVGSFMF
                     KYLQAIARSYGISGFTAEVLVENKPMQSVFNHSGTTIKLRCEDNVYYYDMEFVGGARS
                     "
     gene            complement(2742..>2936)
                     /locus_tag="VJC18_08895"
     CDS             complement(2742..>2936)
                     /locus_tag="VJC18_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45540.1"
                     /translation="LAEYLEIEKKSLEAIMSSFKTLPEIFNAGLVRCLLPQEMKNRAE
                     KIFTKRYKQIFGQLSVTGNN"
BASE COUNT          718 a          668 c          826 g          724 t
ORIGIN      
        1 cagtgccgat gacgccatag aggcagcgga acaggcaggg tttcccgtgg ccatgaaaat
       61 cgtatcacct gatattcagc ataaatccga ttttggtggg gtgaagctgg ggctcaataa
      121 tcgggaagag gttcgcgatg cctacgacct gatgatgctc agaatcacgc gtcgtgtgcc
      181 agaagccgtc atcaccggag tctatgtcga aaaaatgtgc cccaagggac gggaggtgat
      241 tttgggaatg tcgcgtgacc cgcagtttgg tccgatgctc atgtttggtt tgggagggat
      301 ttttgttgag gtcatgaaag atgtcacgtt ttatttggca cccatcaccg aagaagagtc
      361 catgcaaatg ctgaggacaa cgaagtcgta tcaattgctc aagggagcga ggggacaggc
      421 ggctgtggat ttgcacgccg tggctgtgtc tttgcagaaa atcagtcagc tggtcacaga
      481 ttttcccatg attcaggaat tggacatcaa ccctttcatg gtggcggctg tgggacaggc
      541 ttcgtttgta gcggatgcga ggatgactct ttgtcgtggc tgaaaaccat ccatctgcgg
      601 cgttgtccgc agcacatgcc gtcctcacgt actagcgtgt acgctccggg ggcatgtttg
      661 cggacgcctt gcatctgaat ggttttgagc cacgacatta gcatagtgac aagaggaaat
      721 tatggaaatg aacaatcatt cttggaaaga aaaatacaaa aacgtcatca tgacagcgga
      781 tgccgcggtg aaacacattc acccggggca acgggttttt gtgggtacgg gctgtgcaca
      841 gccgcaacag ttggtcaaag ccttggtgag tcgtgctgac gaactggttg ataccgagat
      901 tgtgcacctc ctcaccattg ggcaggcccc ttacgcgcac aaagagctgg cgcagaaatt
      961 tcaggtgaat agttttttca tctccgacaa tgtgcgcgat gtgatccagg aagggttggg
     1021 cgattacacc ccgatttttt tatcggacat cccagagctt ttcaaaagcg gccagatgcc
     1081 tttggatgta gctctgattc aggtgtcccc tccggatgaa aagggaaact gctcgctggg
     1141 cgtttcggtg gatatcgtca agagcgcggc acagaatgcg cgggttgtga tcgcgcaggt
     1201 gaacgaacag atgccacgaa ccttgggcga cagtttcttg catgtgcacg atttcgatta
     1261 tctggtgccc gtcgatgtgc ctctcatcga ggtcacttgg ggagggttga cggaagaaac
     1321 aacacaaatc gggagttatg tggcctcgct catcgatgac ggcgcaaccg ttgagttggg
     1381 gattggtcgc gtgccacacg ctgtcttgtt atcgctcaag aacaaaaaag atctggggat
     1441 tcacaccgaa atgttttcgg atgccatcat cgacatgatt gagtctggtg ctgtcaatgg
     1501 ttccaaaaag agcatggaca agggcaaggt gacagccagt ttttgtctgg ggacaaagcg
     1561 gctttatgat tacatccaca acaaccccgc gtttgccttt cacccaactg aacatgtgaa
     1621 cgatccccat gtcatcagtc agcaatacaa gatggtggcc gtcaacgtgg ccttggaagt
     1681 tgatctgacc ggccaggtct gtgctgattc gctgggtacg aagttttatt ccggcatcgg
     1741 tgggcaggtg gattttaacc gtggggccgc gcgctgtaaa gacggcaagg cgatcattgc
     1801 catggaatca accgcgcttg atggtaaagt ctcgcgcatc gtgtcgcgtt taacacccgg
     1861 tgctggcgtg gtcacatcac gtggtgatgt gcactatgtt gtgacagaat acggtgtggc
     1921 gtatttgcac ggcaagaaca tccagcaaag agccttggcg ctcatcagca tcgctcatcc
     1981 cgattatcgc gaacaacttt tgaaagaggc gattgcgtac aaatacatcc gcgctgatct
     2041 ggccagtgtg ggagacagga ttcatgtggg ccccaaagaa atgcgaacaa cgcaggttct
     2101 ggatgatggc acgcaggtgt tttacagacc cattcacccc accgacgaac ccaaaatcaa
     2161 ggagctcatg tacagcttgt cacaaaaatc cctgtacacg cgcttcatga gccgcttgcg
     2221 ctgggtttcg caaaagcagg tgcgtgaatt catttatgtg gatcatcgaa acgaggtgga
     2281 gatcgtggct tgtctccctg aggcgtcggg agattgtgtg ctcgcgattg gcgggtatta
     2341 tctggatccc aaaacaaaca gggccgaagt ggcgttcatt gtgcgcgacg agtggcagaa
     2401 cagacacgtg gggtcattta tgttcaaata cctccaggcc attgcccgca gttacggtat
     2461 ttccgggttt acggcagaag ttctggtcga gaacaaaccg atgcagtctg tttttaacca
     2521 cagcgggacc acgatcaagc ttcgttgcga agataacgtg tattattatg acatggaatt
     2581 tgttggcgga gcgagaagtt aggcggtcgc catttgtttc ttttggctgt ttatgatagc
     2641 cgcacgcatc gcaagacgga ggcgttttca ataaacaaaa aactaaaaat cgctgtgcag
     2701 ggcgcacaaa atcaaaactc gcaggtttcg cagctcaata ttcagttgtt cccggtgacg
     2761 gaaagctgac caaaaatctg tttgtatcgt tttgtaaaga ttttctctgc acgatttttc
     2821 atttcttgtg gcaacagaca gcgaacgagg ccagcattaa aaatttctgg caacgttttg
     2881 aacgatgaca tgatggcctc gagggatttt ttttcgatct cgagatattc tgcaag
//
LOCUS       DATDUN010000635         1258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_197692, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000635 DATDUN010000000
VERSION     DATDUN010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197692"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..>1258
                     /locus_tag="VJC18_08900"
     CDS             158..>1258
                     /locus_tag="VJC18_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45541.1"
                     /translation="MFTHLLEHCPDPVRLFDNIIAVNNGQAIVWKFGRWFSDWVAADI
                     SKRLEHELSSTALKHDHQLFVQKITQSLTALGHVQATIDAHQTQGRLTANDVLKVCDL
                     SVRILFDALSKFSLEHSAGIMIAVGIIAALDALNTARIQINASDVDATTVTTTVKALA
                     ELCVACDALVHAEEILAAIPSLTTWRRGFALDDAQRSGLVDQMIVIGANRPECRERVV
                     GWFDAHTILSGNTISDFAPDIIQRLTVSFALSADDPRRSFASWYLERTEKKQYFDLTT
                     MVRSRLTSAERRDIEQLLPDVEARARALVPTVSHLGGVIWFRQVPSAASVAINTLRDQ
                     AIVVGHIMDGDERLEGGFRETIHNHPSPDDVAM"
BASE COUNT          310 a          295 c          320 g          333 t
ORIGIN      
        1 cgaatgtgct cggacacttc gtgtgaaaga gttcttccat caatgcgcat gacctcttct
       61 agcgtaaaat aataaatgag gcaatgtatt tgttagataa accaaggact ggttagtagg
      121 tgtatgctgg aattggagta gccaaagcac tgatcggttg ttcactcact tgttggaaca
      181 ttgtccggat cccgtgaggt tgtttgataa tatcattgcg gtcaacaatg ggcaggccat
      241 tgtttggaaa tttggtcgct ggttttctga ttgggtggcc gccgatattt caaaaagact
      301 cgagcatgaa ttatcgagca cagcattaaa acatgatcat caattatttg tgcaaaaaat
      361 aacgcaaagc ctgaccgcgt tgggacacgt tcaagccacc attgatgccc atcagacaca
      421 aggacgcctt actgctaatg atgtccttaa ggtgtgtgat ctctcagttc gaatcttgtt
      481 tgatgccttg tcaaaatttt ccctggaaca ctctgccgga atcatgattg ctgttggcat
      541 catcgcggcg ttggatgcat taaacacggc aaggatccag atcaacgcat cagatgttga
      601 cgcgaccact gtgacaacga ccgtcaaggc attggctgag ctttgtgttg catgtgatgc
      661 cctggttcac gccgaagaga tcttggcggc gattccctct ctcaccacat ggcgacgggg
      721 atttgctttg gatgacgcgc aaagatctgg actcgtggat cagatgatcg tcattggggc
      781 caatcggcct gagtgcaggg aacgcgttgt tggctggttt gatgcccaca ccattttatc
      841 gggaaatacg atatcggatt ttgcaccaga catcatccaa agattgactg tctcatttgc
      901 cctttcagct gatgatcccc gtcgctcttt tgcctcttgg tatctggagc gaacagaaaa
      961 aaaacaatat ttcgacctga caaccatggt ccgctctcgc ctgaccagcg ctgagagacg
     1021 cgacatagaa cagcttctgc cagatgttga agcgagggcc cgggcattgg tgcccactgt
     1081 ttctcacttg ggcggcgtga tttggttcag gcaggtgcca tctgccgcat cagtggctat
     1141 taacacactt cgcgaccaag ccattgttgt cggtcacatc atggatggcg acgaacggtt
     1201 agaaggtggt tttagagaaa ccatccataa ccacccatcg ccagacgatg tggccatg
//
LOCUS       DATDUN010000636         1006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_566248, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000636 DATDUN010000000
VERSION     DATDUN010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566248"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..361)
                     /locus_tag="VJC18_08905"
     CDS             complement(134..361)
                     /locus_tag="VJC18_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45542.1"
                     /translation="MYDVDTILGRLKLSQQLVDKIKKEARQEFPHDEMMFELHVLRTA
                     NAIKHGLLTPNKSGNALQDNPLPSEGACQST"
     gene            complement(372..830)
                     /locus_tag="VJC18_08910"
     CDS             complement(372..830)
                     /locus_tag="VJC18_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLD45543.1"
                     /translation="MRNLEKIYLDTSVFSAFYDSKTPQRQEQTVQFWNKLSQYQNFIS
                     TIVIDEINAVSDVALKQKLLALTQNHFQILAVTQDSEYLAKQYVQEGIFPLKYFDDAL
                     HLATASINQIDIVLSWNFAHFVKRKTRILANLVNNKAGYRSVEIIAPPEL"
BASE COUNT          253 a          177 c          285 g          291 t
ORIGIN      
        1 gggcaaaggg gcaaagggtc aatgggggga gggggataat tgcgcaattg ttgcaaccgg
       61 caattgccgc aatggcctgt gtaccagtta tcggtacagg gtggcgcttt tttgtccgat
      121 tggggactgg caactatgtt gattgacagg caccttctga tggaagcgga ttgtcctgca
      181 aagcgttgcc agatttgtta ggggtaagca aaccgtgttt gatggcgttg gctgttctta
      241 atacgtgcaa ctcgaacatc atttcgtcgt gcggaaattc ttgacgagct tcctttttga
      301 tcttgtcaac caattgttgg ctgagcttga gcctgcctaa gatggtatcg acgtcgtaca
      361 tggggaacct cctacagttc tggtggggca ataatctcaa cactcctgta tcctgctttg
      421 ttgttgacca aatttgccaa gatacgtgtt ttgcgtttta caaaatgtgc aaaattccag
      481 ctaagaacaa tgtcaatttg attgatgctg gctgtggcca aatggagggc gtcgtcaaaa
      541 tacttcagcg ggaatatgcc ttcttggaca tactgttttg caagatattc cgaatcttgt
      601 gtcacagcaa gtatttgaaa atggttttgt gtgagagcga gtagtttttg tttaagggca
      661 acatcgctca ccgcgttgat ttcgtcgata acaatagtgg aaataaaatt ttggtattga
      721 ctgagtttgt tccaaaattg aactgtctgt tcttgtctct gtggagtttt tgaatcatag
      781 aatgcgctga acacggaagt gtcgagataa attttttcga gatttctcat gaaggttagc
      841 ttaccaattt catagggata tgacaagatg atatcgaaaa accgtgcagt gttacggcgg
      901 acaggtcggc aggatggggg aaggggaagg cgaaaggctc aaggcgtaag gcgaaagggg
      961 ggaagggggg ggcaattgcg caattgttgc aactggcaat tgtggc
//
LOCUS       DATDUN010000637         1925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_443746, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000637 DATDUN010000000
VERSION     DATDUN010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443746"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VJC18_08915"
     CDS             <1..471
                     /locus_tag="VJC18_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLD45544.1"
                     /translation="PLIHGAGHEGGRRAGTENVIFDAGLGCACQLAQFLLDDHTVGEL
                     TQRFYHELSKAFGSCIRLNGHPKNRLPNTLNISFLGSGQVAVQKALARVATSAGSACH
                     AGSVTISPVLRAMGIMPDELGTVVRFSLGRFTTQHDIDTVLDDLSMLVSGQACV"
     gene            513..833
                     /locus_tag="VJC18_08920"
     CDS             513..833
                     /locus_tag="VJC18_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HLD45545.1"
                     /translation="MKFGIILETKEPEKAWNALRFAVASIKNKHEVRLFLMGEAVECE
                     NLLHEKYNVAAQIKEFTENGGNILACGTCLKSRQLNASETCPLSTMVDCVQMVEWADR
                     VLTF"
     gene            929..1567
                     /locus_tag="VJC18_08925"
     CDS             929..1567
                     /locus_tag="VJC18_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLD45546.1"
                     /translation="MQGNKNSGFIFAMLAALFFGLSVPVSKILLGDVSPWLLAGLLYA
                     GPGAGLFAVILFRYFLRISLSVETPIRGKKWLRLGGATFFGGVVGPALLLFGLTRTQA
                     SATSLLLNLESVLTILIARIIFREHVGLRVTIGSMLTILGCLILSWTNHFSLESLTGP
                     MFILAACAAWAIDNNLTRKISASDPLQIAMLKSLMAGATNILLAMTACLFPR"
     gene            complement(1689..>1925)
                     /locus_tag="VJC18_08930"
     CDS             complement(1689..>1925)
                     /locus_tag="VJC18_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45547.1"
                     /translation="KPNGMTVADSMALAVIGTATPDEDYNYPDENSYEHPKSMRLLFN
                     NDCEYERGTLPGWGTQCSTDDDDDYIDLEITGSE"
BASE COUNT          461 a          441 c          477 g          546 t
ORIGIN      
        1 ccgctgattc acggcgctgg acatgagggt ggccgacgcg caggaacaga aaatgtaatt
       61 tttgatgctg ggctgggatg cgcctgtcag ttggcacaat ttctgcttga tgaccataca
      121 gtgggggagc ttacacagcg gttctatcat gaactcagca aagcgtttgg cagttgcatc
      181 cggcttaatg gccatccgaa aaaccgatta ccgaatacat tgaatatcag ctttttgggg
      241 agtgggcagg tggctgtcca aaaggcactg gctcgtgttg ccacatcagc agggtctgcc
      301 tgtcatgcag ggtctgtgac aatttcgccg gttttgagag cgatgggtat catgccagat
      361 gagcttggaa cagttgtccg ctttagcttg ggccgtttca ccactcaaca cgacatcgac
      421 actgttcttg atgatctatc gatgcttgtt tccggacagg cctgtgttta ggccagacag
      481 acaaagcact aacagagacc gtggaggaca ttatgaaatt tggaataata ttggaaacca
      541 aagagcctga aaaagcctgg aacgcgctca gatttgctgt ggcatccatc aaaaacaaac
      601 acgaagtcag gctatttctc atgggtgaag cggtggaatg tgaaaacctg ttacacgaaa
      661 aatacaatgt cgctgcacaa atcaaggaat tcacagaaaa cggcggcaac attctggcct
      721 gtgggacatg cctcaagtca cgccaattaa acgcctctga aacttgtcct ctttctacaa
      781 tggtggactg cgtgcaaatg gtcgagtggg cagatagggt tcttactttt tgatggcggt
      841 catatcaaag gttcttgttt ttccacccaa cgcgctatcc tgaaaacaaa caaagacatt
      901 ttgaactatg caatttcaga tgctcaatat gcagggcaac aaaaacagcg gttttatctt
      961 tgcaatgctt gccgcgttat tttttggtct cagcgttccg gtttcaaaga ttctgcttgg
     1021 cgatgtctcg ccttggctct tggcagggct tctttacgct ggcccaggcg cgggtctttt
     1081 tgccgttatc ttgtttcgat acttcctgcg catttccctt tcagtcgaga cgccgattag
     1141 gggaaaaaaa tggctgaggc ttggtggagc aacctttttc ggaggcgttg tgggacccgc
     1201 gctacttttg tttggcctca cacgaacaca agcttcggca acatccctgt tgctcaatct
     1261 ggaaagtgtt cttaccattc tcattgcccg gatcattttt agagaacacg tcggccttcg
     1321 agtgacaatt ggttccatgc tcacaatttt aggctgtctt atcctttcgt ggacaaatca
     1381 tttcagcctt gaatcattga caggtccaat gttcatcttg gccgcttgcg ccgcgtgggc
     1441 cattgacaat aacttaacga gaaaaatatc ggcaagcgac ccgttacaga tcgccatgtt
     1501 aaaaagtctt atggctggcg caaccaacat tttgttagcg atgacggcat gtttatttcc
     1561 tcgatgattg gttggggctt aaatcacatc aacaagtctc tggtgggttt tattctagtt
     1621 gacacgaatt gctaatgggt gaccccttgt ccttctcgaa tgatctgtat gtggagtagg
     1681 cagacctgtt attcgcttcc tgtgatttcc aagtcgatgt aatcgtcatc atcatccgtt
     1741 gagcattgtg taccccaacc aggtagtgtg cctctttcgt actcacaatc gttgttaaac
     1801 aacagacgca tggattttgg atgttcgtaa gagttttcat cgggataatt gtaatcctcg
     1861 tcgggtgttg ctgtgcctat cacagctagg gccatgctgt cggcaacggt catgccattg
     1921 ggctt
//
LOCUS       DATDUN010000638         1606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_443750, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000638 DATDUN010000000
VERSION     DATDUN010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443750"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..756
                     /gene="rph"
                     /locus_tag="VJC18_08935"
     CDS             40..756
                     /gene="rph"
                     /locus_tag="VJC18_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HLD45548.1"
                     /translation="MIPTSRNQDRKPDQLRKTTITSGFTKYAEGSVLITMGDTKVLCT
                     ASLEEKVPPFLKNTGKGWVTAEYGMLPRSTNERMEREAKKGKQSGRTQEISRLIGRAL
                     RASLNLDLLGERQIIIDCDVLQADGGTRCASITGAYVALCHAVRFLLSNNKIKTDPLL
                     TPVCAVSCGIINGVPSLDLDYNEDSSAEVDMNFVITGDYNFVEVQGTAEHKPFTFEQL
                     VKMKDLALKGGRELFVAQQL"
     gene            753..1361
                     /gene="rdgB"
                     /locus_tag="VJC18_08940"
     CDS             753..1361
                     /gene="rdgB"
                     /locus_tag="VJC18_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HLD45549.1"
                     /translation="MKTLYIASKNAHKVREIAQMLQHLALTVKSLITFPDYISPEETG
                     ASFIENARIKALSLKTYLEQRQITACVLADDSGLSCDGLGGLPGVASARFAGPQAHDT
                     ENNEKLVLAFSTNPTLNRSAYYTCALVLLLPDGTQKEIEEHCCGEIQTQAKGKNGFGY
                     DPYFFIKDYQLTMAELAPDEKNRISHRGKALRKLLEHLSQIC"
     gene            1372..>1606
                     /locus_tag="VJC18_08945"
     CDS             1372..>1606
                     /locus_tag="VJC18_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45550.1"
                     /translation="MPYLIPHIILGFYLMATLLYLLRTIYDRPALTALGLRLVFGAAL
                     AQTCFLIWHFVKIRGAWPHGYFEFFQLTSLLLAY"
BASE COUNT          398 a          439 c          377 g          392 t
ORIGIN      
        1 gtgttacaaa caagacatta ttaacaggag ggctgtctca tgatccccac atccagaaat
       61 caggatcgca aacccgacca gcttcgcaaa acaaccatca cctcgggttt taccaaatac
      121 gcggaaggct ctgtgctcat caccatggga gacacaaagg tgctctgcac cgccagcctt
      181 gaggaaaagg tgcccccttt tttaaaaaac acgggtaagg gctgggtcac ggcagagtac
      241 ggcatgttgc cacgctctac caatgagcgc atggagcgcg aggccaaaaa ggggaaacag
      301 tccggccgca cacaggagat ttcacgcctg atcgggcgtg ccttgcgggc ctcgttgaat
      361 ctcgatttgc tgggtgagcg acaaatcatt atcgactgcg atgttctgca ggccgatgga
      421 ggaactcgtt gtgccagcat taccggcgcg tatgtggccc tctgtcacgc tgttcgtttt
      481 cttctcagta acaataaaat caaaactgac ccactcctta cccccgtgtg tgccgtgagt
      541 tgtggcatca tcaatggcgt tccctcgctt gatctggatt ataacgagga ttcctctgcc
      601 gaggtcgaca tgaactttgt catcacgggt gattacaatt ttgtggaggt tcagggaacg
      661 gccgagcaca agcccttcac gtttgagcaa ctggtcaaaa tgaaagacct cgcgctaaaa
      721 gggggcagag aacttttcgt cgcgcaacaa ttatgaaaac cctctacatc gcatcaaaaa
      781 acgcgcacaa ggttcgtgag atcgcgcaaa tgctgcaaca cctcgctctc actgtaaaat
      841 cgctgatcac gtttccagac tatatttctc ccgaagaaac aggtgcctcg ttcatcgaaa
      901 acgcacgcat caaggcactg tctctcaaaa cttatctgga acagcgccag atcactgcct
      961 gcgttctggc ggatgactcc ggtctgtcct gtgatggcct tggcggtctt cccggtgttg
     1021 cttcggcacg ctttgccggc ccacaagcac atgacactga aaacaacgaa aagcttgtcc
     1081 tggctttctc caccaatccg accctgaatc gatccgctta ttacacgtgt gcattggttt
     1141 tgctgttgcc tgacggaaca cagaaagaga tcgaagaaca ctgttgtgga gaaatccaaa
     1201 cgcaggcaaa aggaaaaaac ggctttggtt atgatccgta tttttttatc aaagactacc
     1261 agttgaccat ggcggaactt gcgcctgacg aaaaaaaccg catctctcat cgtggcaaag
     1321 ccctgagaaa actgctggag cacttgtccc aaatctgcta aaacgcaatg cgtgccctac
     1381 ctcattcccc acatcattct tggtttttat ctcatggcca cactcttgta tctcctgcgc
     1441 accatttacg accggccagc gctaacagcc ctgggacttc ggcttgtgtt tggtgccgcc
     1501 ttggcccaaa cctgttttct gatctggcat tttgttaaaa tccgtggtgc ctggccgcat
     1561 ggttattttg agttttttca actcacatct ctactcctgg cctatg
//
LOCUS       DATDUN010000639         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_319087, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000639 DATDUN010000000
VERSION     DATDUN010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319087"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VJC18_08950"
     CDS             <1..330
                     /locus_tag="VJC18_08950"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HLD45551.1"
                     /translation="ERAEFYKIGTRKAQAISKVMGCFRLVCDDSRRVSLFRAALGSVS
                     ATPVRLSETEKFLVGQMLSEQIVTQAISIAANEISPIDDVRSTAEYRRHCAGVLLERF
                     LRADSKL"
     gene            351..1379
                     /locus_tag="VJC18_08955"
     CDS             351..1379
                     /locus_tag="VJC18_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45552.1"
                     /translation="MTFIFVVLFIPEAHALEADQYVIWTKSDKLKDATPQINALTNQV
                     LQEFLDRYVNNEEALERNPSCESLSVRFLLYVRPHFFLDRIKGGLVDDPQIELSPQKT
                     DLFKDYSSSIYKGFRWPFVMPVAQTIFINGVYLGTDKIDHFFSSGRRYFNVYRRARKQ
                     GLSHEEAIKKALDFGLSVFEEKGVLGYWTSGAFSYADIEANFQGMMLGINMCKAESPH
                     LAYDALVRKWYINKPIEWQEYVSPLWDEAYNNSYYFSYRWNGVKKVLEEVYCKLGLDG
                     FARSLWKSYDESVRVDEHIRYLQELLQRGKIPDPKKQSLHEVCRYPAGVMEGAPFWDT
                     KMLPKEND"
     gene            1433..1657
                     /locus_tag="VJC18_08960"
     CDS             1433..1657
                     /locus_tag="VJC18_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45553.1"
                     /translation="MAKKKSSEKKSCKKCEMLLVGSKTKDALRAQGCNVAGDALEALN
                     GLLYWYIEQAAKRAQANGRKTVRGHDFCVC"
     gene            1713..>2541
                     /locus_tag="VJC18_08965"
     CDS             1713..>2541
                     /locus_tag="VJC18_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HLD45554.1"
                     /translation="MIKAPTFVKFRLQDQPLVSGLAETLGVSTVLARILVNRGIATSE
                     DANKFLHTDVSALHSPFLLKDMDVAVARIVRAVSVKERICVYGDYDVDGAVATALLML
                     FFQELGIEIDFYVPNRLTEGYSIHKDSLRPLKENGVTLVITVDNGISAHEAIAHAREL
                     GMDVIVTDHHQAPETLPPALAVVNPNRKDCSYPFKGICGAGVAYKLVMALRQRLREQN
                     YFKGRTEPNLKQYLDLVCLATICDVVPLKDENRFFVKEGLKQLGQTRRVGLIALKAIC
                     "
BASE COUNT          605 a          559 c          707 g          670 t
ORIGIN      
        1 gaacgggccg agttttacaa aattggaaca cgcaaggcgc aggccatttc caaggtcatg
       61 ggatgctttc gactggtttg tgatgatagc cgacgggtgt ccctgttcag ggccgctctg
      121 gggagcgtgt cggccacccc cgttcgtctc agtgagacgg aaaagttttt ggtgggacag
      181 atgttgtctg aacagattgt gacgcaggcg atctccattg ccgctaatga aatttcaccg
      241 attgacgatg tccgttccac ggcggagtac cgcaggcact gtgcgggagt tctgctggag
      301 cggtttttaa gggcagattc aaagctgtga tgcgaaatag aattttaata ttgaccttca
      361 tttttgtcgt cttgtttatt cccgaggcac acgcccttga ggctgatcaa tacgtgattt
      421 ggaccaagtc tgataaactg aaggacgcga cgcctcagat caacgctttg accaaccagg
      481 tgttgcagga atttcttgat cgctatgtca acaatgagga ggctctcgag agaaacccct
      541 cctgcgaatc cctgtcggtt cgttttctat tatatgtcag accgcatttt tttctggacc
      601 ggatcaaggg cggcttggtt gacgatcccc aaattgagtt gtccccgcag aagacggatt
      661 tattcaagga ttattcaagc agtatttata aagggtttcg ttggcctttt gtgatgcctg
      721 tggcacaaac catttttatc aacggggtgt atctgggtac ggacaagatc gatcatttct
      781 tttcatcggg acgccgctac ttcaacgtgt accgccgggc ccgcaaacag ggactgtcac
      841 acgaagaggc catcaagaag gcccttgatt ttggtctgag cgtgttcgaa gaaaaagggg
      901 tgttgggata ttggacatcg ggggcttttt cgtacgcaga tattgaggca aattttcagg
      961 ggatgatgtt gggtatcaat atgtgtaagg ccgagtcccc tcatttggct tatgatgcgt
     1021 tggtgcgcaa gtggtatatc aataaaccca ttgaatggca ggaatacgtc agtccgttat
     1081 gggatgaggc ctacaacaac agctattatt tttcgtatcg ttggaatggg gtcaagaaag
     1141 tgcttgagga ggtctattgc aagttggggc tcgatggttt tgcgcgctca ctctggaaat
     1201 catatgatga gagtgtgaga gtagatgaac atattcgcta tcttcaggag ttattgcagc
     1261 ggggaaaaat ccctgatcca aaaaaacagt cattgcacga ggtgtgtcgc taccctgctg
     1321 gcgtgatgga aggggccccg ttttgggaca caaaaatgtt gccaaaagaa aatgattagg
     1381 ataccccttt ggcacctcgt aaaggggaat ttcacttaac aaggagttta acatggccaa
     1441 gaaaaaatct tccgaaaaaa aatcatgcaa aaaatgtgag atgcttttgg ttggtagcaa
     1501 gaccaaggac gctctcagag ctcaaggttg caatgtggct ggggatgccc tcgaggcatt
     1561 gaatgggctc ttgtactggt acattgaaca ggccgccaaa cgtgcgcagg ccaatggacg
     1621 caagactgtg cgcggtcatg atttctgtgt ctgctgatcc ctcctgatcg cgataccgtt
     1681 tacccagtca tgtttccttg ttacgtccga ctatgatcaa ggcgccaaca ttcgtcaaat
     1741 ttcgtctgca ggatcagcct ctggtgtcgg ggttggcgga gacgttgggt gtctcaacgg
     1801 tattggcccg gattctggtc aaccgtggca ttgcgacaag tgaagacgcc aacaaatttc
     1861 tgcacacgga tgtgagcgcc ttgcactccc cctttttgct caaggacatg gatgtggcgg
     1921 tggcgaggat tgtgcgagcc gtttctgtca aagaacgcat ctgtgtttat ggggattacg
     1981 atgtggatgg tgccgtggcg acggcacttt tgatgttgtt ttttcaagag ctcggaattg
     2041 agatcgattt ttacgttccc aatcgtctga cggagggtta ttccatccat aaagattccc
     2101 tgcgaccact taaggagaac ggtgttacat tggtgatcac agtcgataac ggaatttcgg
     2161 cgcatgaggc catcgcgcac gcgcgtgaat tgggtatgga cgtcattgtc accgaccatc
     2221 atcaggcacc agagactctg ccgcctgctt tggcggtggt gaatccaaac cgcaaagatt
     2281 gttcgtatcc cttcaaaggg atttgcgggg cgggtgtggc ctacaagctg gtcatggccc
     2341 tgcgccagag actgcgcgaa cagaattatt tcaagggacg taccgagccc aacctcaaac
     2401 aatacctcga ccttgtgtgt ctggccacca tctgtgatgt ggtgcccctc aaggatgaaa
     2461 atcgtttttt tgtgaaggaa ggcttaaagc aattgggaca gacgcgacgc gtcggcttga
     2521 ttgcgctcaa ggccatttgt g
//
LOCUS       DATDUN010000640         3712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_612178, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000640 DATDUN010000000
VERSION     DATDUN010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612178"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..491)
                     /locus_tag="VJC18_08970"
     CDS             complement(9..491)
                     /locus_tag="VJC18_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45555.1"
                     /translation="MFAKEPVQLYTLIHQFSNIVENKDELGSIISYVLVSTLMEFSAQ
                     AGSWQEMQVEQIAAIYQGLEDTLDQCRSSDSYQILCALNVKVHEFLKTVETEKDIVAN
                     PLLKHIMTKLANKRGVPADTFRRSGLALVNAIIERGALTRKVDCLKQDYRIIEVIFAT
                     "
     gene            complement(702..2129)
                     /locus_tag="VJC18_08975"
     CDS             complement(702..2129)
                     /locus_tag="VJC18_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLD45556.1"
                     /translation="MGNKFHKAKHTLVLSDIHLTIAQPERPRRPLWKRFNRKEFFIDD
                     RFAVLLAQATENLKKDIELILAGDIFDFDSVVTIPPNPTFAVSWLEKKRGLNSEEPKS
                     LYKIDLIIQNHPIFFQALRDFLQAGNKLVFIIGNHDIELHWPSVKQRIIDNLVLEDSQ
                     KELVRFCNWFYISNSDTLVEHGNQYDPYCSVINPINPLYRRPSATFLRVPFGNLVHRY
                     MINGMGIFNPHVDTTYLLSFGGYLRFYVNTIRTQPFLIFDWLFGAIATWVVSIRDSIM
                     PRIKNPLTLEAQIEDIAQKANAEPSMVRTLRELQAHPVSWDIIKIMRILWLDRALLFL
                     LLVFCAFQFVGLMHIFWGVSFGWALLVLLAFTPLYLLYVHNLETIRPVTKFAISKQIV
                     TLATQITKTERMICGHTHAPTHKIISEVEYINAGYWSPSFKDPNCSVFEKNRSFVWIR
                     PGFRKREAKLLFWDDRASLENPFEI"
     gene            2265..2987
                     /locus_tag="VJC18_08980"
     CDS             2265..2987
                     /locus_tag="VJC18_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45557.1"
                     /translation="MTTNRKTSARCFVIEYHISMPDGEEKRICFSLDAQTLVMQNQQR
                     ASYPDWTKLDCHQCPHCPFDPETRVHCPVAVNIHEVVTIFASSISYEEVNVRVITEER
                     EYSRKVPMQKAVSSLLGIGMVACDCPIMAKLKHMVRFHLPFATPLETLQRTLSSYLLA
                     QYFRKKEGKTPDWNLAHLKKIYEDIRKLNVAMRDRLLATGKGDANVNAIVRLDVLAIT
                     ATDFLEGNLIGHLEGYFDPYLK"
     gene            complement(2997..>3712)
                     /locus_tag="VJC18_08985"
     CDS             complement(2997..>3712)
                     /locus_tag="VJC18_08985"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLD45558.1"
                     /translation="YAGANLCVRGALHMSKTMHFIYLCLFVIFFVGFGFVAMKQADLS
                     KKYYSYLSTDEPTTNQPGTSNAQLETLNKKLTEQQQEISSLNDKITELTQSGLADLSD
                     KYMPAPEFATKEPVFSIREESKQTFKVTGFTKPLADWQQRNELFFEYVLQKIIPFEYD
                     RRTQSIAVLDVMENSVFYQMGLRKGDRILRVNGQPFIKGMDLRYKLIEPKNKKIQIMR
                     DKQNILLDVAYQTTADNPA"
BASE COUNT          924 a          816 c          976 g          996 t
ORIGIN      
        1 cactcctctc acgttgcgaa aatgacctca atgatccgat agtcctgttt gaggcaatcc
       61 actttgcgcg tcaaggcccc gcgttcgatg atggcgttga ccagtgccaa cccgctcctt
      121 ctgaaggtat ccgcgggaac ccctcgttta ttggccagtt tggtcatgat gtgtttgagc
      181 aaagggtttg cgacgatgtc tttttctgtc tcgactgttt tgagaaattc gtgcaccttg
      241 acgttgaggg cacacaggat ttgatagctg tctgaactgc ggcattgatc gagcgtgtct
      301 tcgaggcctt gataaatggc ggcaatctgt tcgacctgca tttcctgcca ggaccctgcc
      361 tgagcagaaa attccatgag ggtcgatacg agcacgtaac tgatgatact gcccagctcg
      421 tccttattct ccacgatatt ggaaaactga tgtatgagcg tgtacaactg gacaggttct
      481 tttgcgaaca cacaaccccc ttacaatgtc tctttggaaa ttttaactga tattggcaaa
      541 gaatgcaaat gacttgcgga catccctgcg cagacaggaa ttcataatga ataacgaata
      601 tcgaacaggg aatgtcgaat gtcgaaattt gtcatctctg cggtggacgc tggaatgaca
      661 atcacctcga tattccttgt tccttgttca tgattcgtta ttcaaatctc aaacggattc
      721 tccaagctgg cgcgatcatc ccaaaacaaa agcttggcct cccgcttgcg aaaaccgggt
      781 ctgatccaaa cgaaggatct gttcttttcg aacacagaac aattggggtc cttgaacgag
      841 ggggaccagt agcccgcgtt gatgtattcc acctcgctga tgatcttgtg ggtgggcgcg
      901 tgtgtgtgtc cacagatcat gcgttccgtt ttggtgatct gtgtggccaa cgtgacgatt
      961 tgtttggaga tggcgaattt ggtgacgggt ctgatggttt cgagattgtg aacgtacaac
     1021 agataaagcg gagtgaaggc cagaagaacc agtagggccc atccaaagga gaccccccaa
     1081 aaaatgtgca tcagtcccac aaactgaaaa gcgcaaaaaa ccaaaaggag aaacaagagg
     1141 gcgcggtcca gccacaagat gcgcatgatt ttgatgatgt cccacgaaac gggatgggct
     1201 tgaagttccc tgagggtcct gaccatgctg ggttccgcat tggccttttg ggcgatgtct
     1261 tcgatttggg cttccagtgt gagagggttt ttgatgcggg gcatgatgct gtcgcggatg
     1321 gagaccaccc atgtcgcgat ggctccaaat agccagtcga aaatgagaaa cggttgtgtt
     1381 ctgatcgtgt tgacgtagaa cctgaggtaa ccaccaaaac tcaacagata ggtcgtgtcg
     1441 acatgtgggt taaaaatccc catcccgttg atcatgtaac ggtgcaccag gttcccaaaa
     1501 gggactcgca aaaaagtggc gctgggtcgt cgataaagag ggttgatggg gttgatcaca
     1561 gaacagtagg gatcgtattg gtttccgtgt tccaccaatg tgtcgctgtt gctgatgtag
     1621 aaccagttgc agaaacgaac gagctctttt tgcgagtcct ccaaaacgag gttgtcgatg
     1681 atgcgttgtt tgacgctggg ccaatggagc tcgatatcgt gattgccaat gatgaatacg
     1741 agcttgttgc ccgcttggag aaaatctcgc agagcctgaa aaaaaatcgg gtgattttga
     1801 atgatcagat caatcttgta gagcgatttg ggttcctcgg aattaaggcc acgttttttt
     1861 tcgagccaac tgacggcgaa agtcgggttg ggagggatgg tcacgacgct gtcgaaatca
     1921 aaaatatcgc ctgcgaggat gagttcaatg tcttttttaa ggttctcggt tgcctgggcg
     1981 agtagcaccg cgaaccggtc atcaatgaag aattccttgc gattgaatcg cttccacagg
     2041 ggcctgcgcg gacgttcagg ttgggctatg gtgaggtgaa tatcgctcaa cacgagtgtg
     2101 tgcttagcct tgtgaaattt attacccatg cggaggggtc tatactgatg atctcctgaa
     2161 taagcaaatc tggaaaaatg acatccagat atttctggat atgaagggtg ttttatgttt
     2221 tgctgtgaac atgtgtgaaa cattaggatg ggggcgtgaa caagatgacg acaaatcgta
     2281 aaacttctgc gcgttgcttt gtaatcgaat accacatctc catgcccgac ggggaggaaa
     2341 agcgaatttg tttttcattg gacgctcaga ccttagtcat gcagaaccag caaagggctt
     2401 cttaccccga ttggactaaa cttgattgtc accagtgtcc acactgtccc tttgatcctg
     2461 aaacgcgtgt gcattgtccc gtagccgtga acattcacga ggtggtcaca atttttgcga
     2521 gcagtatttc ttacgaggag gttaacgtgc gtgtgatcac cgaggagcgg gagtattctc
     2581 ggaaggttcc catgcagaaa gcggtcagtt cattgctcgg gataggcatg gtcgcttgcg
     2641 actgtccgat catggccaag ctcaaacaca tggtgcgttt tcatttgccc tttgccacac
     2701 cgttggaaac gttgcagagg acgttgtctt cgtatttgtt ggcgcaatac tttcgaaaaa
     2761 aagagggcaa aacacctgat tggaacctgg cgcatctcaa aaaaatttac gaagacatca
     2821 gaaagctcaa tgtggccatg agggatcgct tgctcgctac aggtaagggc gacgccaacg
     2881 tcaacgcgat cgtcaggctg gatgtcttgg ccatcaccgc aacagatttt ttggaaggaa
     2941 acctgattgg ccatctcgaa gggtattttg atccgtatct caaataaacg cgcaagctat
     3001 gccggattat ccgcagtggt ctgataagcg acgtccaata aaatattttg tttgtctctc
     3061 atgatctgga tcttcttgtt cttgggttcg atgagtttgt aacgcagatc cattcctttg
     3121 atgaagggtt ggccattgac cctcaggatg cgatcgccct tgcgcaagcc catttgataa
     3181 aaaacggaat tttccatcac atcaagcacc gcaatggatt gcgtccggcg gtcgtattcg
     3241 aaggggatta ttttttgtaa aacgtattca aaaaataact cgtttcgctg ttgccaatcc
     3301 gcgagcggtt tggtgaaacc ggtgaccttg aaagtctgtt tggactcttc ccgaatggag
     3361 aaaacgggtt cttttgtggc aaattctggc gcgggcatat atttatcgct caaatcagcg
     3421 aggccacttt gtgtgagttc ggttatttta tcgttcaggg agctgatttc ctgctgttgc
     3481 tcggtgagct ttttgttcag cgtttccagt tgcgcgtttg acgtgccggg ttgattggtt
     3541 gttggttcgt ctgtggacag gtaagaataa tattttttgc tcaaatcggc ctgcttcatg
     3601 gccacgaagc caaaaccaac gaagaaaatc acgaacagac aaaggtagat gaaatgcatt
     3661 gttttactca tgtgcagagc tcctctcacg caaagatttg ccccagcgta ct
//
LOCUS       DATDUN010000641         2385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_284221, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000641 DATDUN010000000
VERSION     DATDUN010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284221"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VJC18_08990"
     CDS             <1..887
                     /locus_tag="VJC18_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45559.1"
                     /translation="LVRLGEKIHENGFFLPEAMNRALAVLLDFKSLIKKHNCRKVRVV
                     ATAGCRGAVNAPAFIQDVRQQTGLSIEIISGEEEASLTHLACKTDFAHLGLPLMVLDI
                     GGGSTEIILDDGKQVIKKSFPFGVVTLTEKFFHHDPPENNEIERCREYIKDNFWKFLP
                     DLGPWTPALSDLSRLLSREAEGLDFGLNLVAPAGTPTTLCAISKNLLEYDSSQVHGTQ
                     MAKTEVVKITKKLEALSFNERCLIPCLPPKRADVIIAGGHILDVVMGFFGLTHFTVSD
                     QGLAFGIIYQLLSKTSIP"
     gene            1118..>2385
                     /locus_tag="VJC18_08995"
     CDS             1118..>2385
                     /locus_tag="VJC18_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45560.1"
                     /translation="MSLIITGLSTVDLESLVGKSNEEILETLRTAMEGMDAEERTTFL
                     EQLPEGLGDITDSLYAKIDGLEEDINALEDDEYMKEKYEEDISELEELVDLADEFESL
                     ATEVNTDITGTEEFYTREVTGSTTVDYSGQVKEGDVITCNIADIATTAMFEDEDSTGL
                     TQYASTITINCGEDQVSLLSYTQSPESATFSVTTKEGITFHIVANGLGNIVFNGQGYT
                     TADALKSWPEDLLKRSYDGTKNMFNNVYGFESEEVVATTEIGEEFVEGFDADMTALNE
                     LLNSSYPISLSSGDPRDSLSPEDQALIKETMLSILGSDLYITDSDISIAEVWEELLSQ
                     IHSKDPLIKNAIMCSLVYLVGKYASDKYNDIFTTNITSTLTESLNYLDNLPNDSNDGE
                     NAMSADAFTKMALIINNQYTTAPAYASVDAL"
BASE COUNT          676 a          588 c          540 g          581 t
ORIGIN      
        1 agctggtgcg attgggagaa aaaatccatg aaaacggttt ttttctgccc gaggccatga
       61 atcgtgcctt ggcggttttg ctcgatttta aatccctcat caaaaaacac aattgtcgca
      121 aggtaagggt ggtcgcgacc gcgggttgtc gcggggctgt caatgccccg gcattcatac
      181 aagacgtaag acaacagacc ggactgagca tcgaaatcat ttctggcgaa gaagaggcca
      241 gtctcactca tttggcctgc aaaactgact ttgctcacct gggattgcct ctgatggttc
      301 tggacattgg tggtggttcg accgaaatca ttctggacga cggaaaacag gtcatcaaaa
      361 aatcttttcc ctttggtgtg gtaaccctca ccgaaaaatt ttttcaccac gacccccccg
      421 aaaataacga aatcgagcgt tgcagagaat atattaaaga taacttttgg aaatttctcc
      481 ccgaccttgg tccttggact cctgccctga gcgatttatc ccgattactg tcgagggaag
      541 ccgaagggtt agactttgga ctcaatcttg tcgcccccgc cggcacgccc acaaccctgt
      601 gtgccatatc gaaaaatctt ttggagtacg attccagtca agtgcatggc acacaaatgg
      661 ccaaaaccga ggttgtcaaa atcacaaaaa aactggaagc actttcattc aatgaacgtt
      721 gtctgattcc ctgtctgcca cccaaaaggg ccgacgtgat catcgcggga ggacacatcc
      781 ttgatgtcgt gatgggtttt tttggtctga cccattttac cgtcagcgac cagggtctcg
      841 cgtttggcat catctatcaa ctcttgtcaa agacctccat tccctaacgt ccttatcaat
      901 cattattgcc ccctcatacc ccaacccctc ttgaacattg ggttattaac acctcatcaa
      961 aaacatctat cccaaccaca aaaaaaccgt tataatataa aatgattgga attctagcgg
     1021 gttattgaca aaaaaacagc cgtgaatttg attttatatt tggcaccccg attgcagtgt
     1081 gattgtttga gcggccaatt tgctcaaggg agatgttatg tcgttaatca ttacgggctt
     1141 aagcacagtt gatctcgaat cactcgttgg taagtcaaac gaagaaatac tagagacact
     1201 tcgtacggcc atggaaggga tggacgccga agaaagaacc acgtttttgg aacaactgcc
     1261 cgagggactt ggtgacatca cggattccct ttatgctaaa atagatggcc tcgaggaaga
     1321 catcaacgcg ctcgaagacg atgagtacat gaaggaaaaa tacgaggagg acatctctga
     1381 gctcgaagaa ctggttgatt tggccgatga gtttgaatct ctggccaccg aggtcaatac
     1441 cgatatcacg gggacagaag aattctatac gcgtgaggtc acaggttcca ccacggtaga
     1501 ctattctggc caagtcaagg aaggagacgt catcacatgc aacatagctg acatcgccac
     1561 taccgcgatg tttgaggacg aagacagcac agggctaacc caatacgcct ccacgatcac
     1621 gatcaattgc ggtgaagatc aagtcagtct tttgagttat acccaatcac ctgaatccgc
     1681 cacattcagc gtcaccacaa aagaggggat tacgtttcac attgtcgcca atggtcttgg
     1741 caatattgtt tttaacggac aaggctacac cacagccgac gcgctcaaat catggcccga
     1801 ggatttgctc aagcgcagtt atgatggcac caaaaacatg ttcaacaatg tttatgggtt
     1861 tgagagcgaa gaagtggtgg ccaccacgga gattggcgaa gaatttgtgg aggggttcga
     1921 tgcggacatg accgccctta atgagctgtt gaattcaagc tatccaatca gtctgtcatc
     1981 cggagacccc cgtgatagcc tcagcccaga agatcaggcc ctgatcaaag agaccatgct
     2041 ttccattctg ggttctgacc tctacatcac agacagtgac atttcgattg ctgaggtttg
     2101 ggaagagtta ttgtcacaga tacatagtaa agaccccctc ataaaaaacg ccattatgtg
     2161 ttcgttggtg tatctggtgg gcaaatacgc ctcagacaag tacaacgata tttttactac
     2221 caacatcaca agcacgctca cagaatctct caactatcta gacaatctgc ccaatgactc
     2281 caatgatggt gagaatgcca tgagcgcaga tgcctttacc aaaatggccc tcatcatcaa
     2341 taaccaatac accacggcac ctgcctacgc cagtgtggat gccct
//
LOCUS       DATDUN010000642         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_490515, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000642 DATDUN010000000
VERSION     DATDUN010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490515"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VJC18_09000"
     CDS             complement(<1..974)
                     /locus_tag="VJC18_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HLD45561.1"
                     /translation="MITNELQLVLNNSLTVAAERRHEYVTLEHILASLITDDEVQKIL
                     RSCGCDLELLKKDILAFLDTLPMVPEAPRMEIQTTIGFQRVVQRAVFHVQSSGNNAVY
                     PYHVLIALYGEKESHAVYFLEKQEITRLLIMEYVSHGLTSEEFDEEEGADDEYEEIEE
                     TRLLNESGAQAEEDEEGGDEETDARSQPRSKSDPLVRFTTNLISKAKAGKIDPLIGRA
                     QEIERIVQILCRRSKNNPVLVGDAGVGKTAVVEGLALAVIHDRVPEILKGMEIYSLDM
                     GALLAGTKFRGDFEERFKAVVNRIKRKKNAVLFIDEIHTIIGAGSTAGG"
     gene            complement(971..1291)
                     /gene="clpS"
                     /locus_tag="VJC18_09005"
     CDS             complement(971..1291)
                     /gene="clpS"
                     /locus_tag="VJC18_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HLD45562.1"
                     /translation="MTKEREESDGQEQAGTMTQPRVREPHMYYVFMLNDDYTTMDFVV
                     HVLQKFFHKTFAEANRIMLHVHHKGRGDCGVFPYDIATTKIAQVHDYARKNEMPLKCI
                     MEKS"
     gene            complement(1416..>2325)
                     /gene="apgM"
                     /locus_tag="VJC18_09010"
     CDS             complement(1416..>2325)
                     /gene="apgM"
                     /locus_tag="VJC18_09010"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HLD45563.1"
                     /translation="HSAGHIATHESHELVNALNDELGSDRVSFYPGVSYRHLLVIKNG
                     NNNIVCTPPHDVPGTQFREVLVKATKPDGQETAGLLNELIMKSQKVLQEHPVNQKRRT
                     QGKDPANSLWPWAAGHKPQMKTLRELYGITGAVISAVDLIKGIGVYAGMKVMEVKGAT
                     GLHDTNYEGKARAAISALRDVDLVYLHIEASDEAGHEGNVALKIKTIELLDERVVKLI
                     VSEVAKMSEPVTIAILPDHPTPCAVRTHTHDPVPFLIYRPSELGDAVSQYDEESVKAG
                     SYGLLTGDGFMRALLNIESFNFVQDR"
BASE COUNT          529 a          658 c          540 g          598 t
ORIGIN      
        1 ccccccgccg ttgaccctgc cccaatgatg gtgtgaattt cgtcgatgaa aagcaccgca
       61 ttttttttgc gtttgatgcg attgaccacc gccttgaacc gttcctcaaa atcgcccctg
      121 aacttggtcc ctgccagcaa agcgcccatg tcgagcgaat aaatctccat tcccttcaaa
      181 atctcgggaa cgcgatcatg aataacagcc agtgccagcc cttctacgac tgccgtcttg
      241 cccacgcccg catcacccac caacaccgga ttattcttgc tccgacggca taaaatctgg
      301 acgatgcgtt caatttcctg cgcgcgaccg atgagagggt caatcttgcc cgctttagcc
      361 ttgctgatca gattggtcgt gaaccggacg agcggatcgc ttttggaacg gggttgggac
      421 cgggcatcgg tttcttcgtc tcccccctct tcgtcttctt ctgcctgtgc gcccgattca
      481 ttcaacagcc gtgtttcttc aatctcctcg tattcatcat ccgcgccctc ctcctcatcg
      541 aattcttctg acgtgagacc gtgtgagacg tattccatga tgagcaggcg cgtgatctcc
      601 tgtttttcaa gaaaatacac ggcgtgtgat tccttttcgc cataaagggc gatcaacaca
      661 tggtacggat acaccgcgtt gttaccactg ctctgcacat gaaagacagc ccgttgtacc
      721 acgcgctgaa atccaatcgt cgtctggatc tccattctgg gcgcctcggg aaccatgggc
      781 agtgtgtcta aaaaagccaa tatatccttt ttgagcagtt ccagatcgca accacaggat
      841 ctgagaattt tctgtacctc gtcgtcggtg atgagggagg ccagaatatg ctccagtgtc
      901 acgtattcat gccggcgttc tgcggctacg gtgagagaat tgttgaggac caattggagt
      961 tcgttggtga tcatgatttt tccattatgc acttgagcgg catttcgttt ttgcgggcgt
     1021 agtcgtgaac ctgcgctatt ttggtcgtgg cgatgtcata tgggaaaaca ccacagtccc
     1081 cacgaccttt gtgatgcaca tgcaacatga tgcgattggc ctccgcaaag gttttgtgaa
     1141 aaaacttctg caggacatgc accacaaaat ccattgtcgt ataatcatcg ttcagcatga
     1201 acacgtagta catgtgtggt tccctgaccc tgggttgggt catggtcccg gcctgttcct
     1261 gcccatctga ttcttcgcgt tctttggtca taatccccta tagaatacac gcaatatggc
     1321 gaaaggacaa gagaaagaat attgaaagaa tatcgacaaa agtacctggc acctaaatct
     1381 ttctaaatct ttcgaccttc gacattccct aaacgctacc tgtcctgaac gaagttgaag
     1441 gattcgatat tcaaaagcgc ccgcataaac ccatcacccg tcagcaatcc ataagagcca
     1501 gccttaacac tctcctcgtc gtattgtgac acggcatccc ccagttcgct gggacgataa
     1561 atcaaaaatg gcaccggatc atgcgtgtgc gtgcgaacag cacacggtgt ggggtgatcg
     1621 ggtaggatgg caatggtcac aggttcggac attttggcaa cctccgacac gatgagctta
     1681 accacacgct catcaagcaa ttcaatcgtt tttatcttga gagcgacgtt cccttcatgc
     1741 cccgcctcat cgctcgcctc aatgtgcagg tagaccagat cgacatcacg caacgcagag
     1801 atggcggccc tggccttgcc ctcatagttg gtatcatgca gaccagtggc ccccttcacc
     1861 tccatcactt tcatgccagc gtaaacaccg atccccttga tcaaatcgac ggccgatatg
     1921 actgcgccag tgatcccgta taattcccgc aacgtcttca tttgcggttt gtgccctgcg
     1981 gcccaaggcc agagagaatt ggcgggatcc ttcccctgcg tcctgcgttt ttgattcact
     2041 gggtgctctt gcaaaacttt ctgggacttc atgatcagct cattgagaag acctgccgtc
     2101 tcttgaccat caggctttgt ggccttcacc agcacctcgc gaaactgggt gccaggcaca
     2161 tcatgtggcg gtgtgcacac gatgttgttg ttcccattct tgatcaccag cagatgacga
     2221 taactcacac cgggataaaa gctcacgcgg tcagagccca gttcatcgtt caatgcattg
     2281 accagttcat gactttcgtg agtcgcaatg tgtcccgccg aatga
//
LOCUS       DATDUN010000643         1087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_401963, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000643 DATDUN010000000
VERSION     DATDUN010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401963"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..>1087
                     /gene="pheS"
                     /locus_tag="VJC18_09015"
     CDS             115..>1087
                     /gene="pheS"
                     /locus_tag="VJC18_09015"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLD45564.1"
                     /translation="MSENIIEQIKHFQSEALSEIRATINLESLNDYRVSFLGKKGRLT
                     EILKTLGTFSPDERKAVGQLANEVKQVLTDAIDEQARVLRAREIARQIQSEAVDVSLN
                     GYGVPAGSHHPVMQIFEQTRTIFQKLGFDIAEGPEIETDFYNFEALNMPLHHPARDMQ
                     DTFYLPDGHVLRTHTSPVQIRVMETQKPPIAIVAPGVVYRSDSDQTHTPMFHQVEGLL
                     VEEHINFGHLKAIIKEYLEQIFEKQLTFRFRPSFFPFTEPSAEVDMSCVICSGKGCRV
                     CKQTGWLEIMGCGMVDPAVFDAVKIDHRKYSGYAFGVGLDRLAMLKYG"
BASE COUNT          260 a          289 c          274 g          264 t
ORIGIN      
        1 aaaaaatctg ctgaccgtct ccaaaacaac acctttccca actgagactg aatcatttct
       61 ttggattcac atttttcctt gccccttgtg ggaaatccga atatttcccg ccctatgtca
      121 gaaaatatta tcgaacaaat caaacatttt cagtcggagg ccctctctga aatcagggct
      181 acaataaatc ttgaatcgct caacgactac cgggtttcgt ttctgggaaa aaaggggcgc
      241 ttgacagaaa ttctcaaaac tctggggact ttttcgcccg atgagagaaa agccgtgggg
      301 cagttggcca atgaggtcaa gcaggttttg accgatgcca tcgacgagca ggcccgggtc
      361 ttgcgagcgc gggaaattgc ccgacagatt cagtccgaag ccgtcgatgt ttcgctgaac
      421 ggttacggcg tgccggcagg cagtcatcat ccggtgatgc agatttttga acaaacccga
      481 acgattttcc agaagctggg ttttgacatt gccgagggtc ccgagatcga gacggatttt
      541 tacaattttg aggccctcaa catgccgctc catcaccccg cgcgcgacat gcaggatact
      601 ttctatctgc ccgatggcca cgtgcttcgc acacacacat cgcctgtgca gattcgtgtg
      661 atggaaacac agaaaccgcc catcgcgatt gtggcccctg gtgtggtcta ccgttccgat
      721 tccgatcaga cccacacacc catgttccat caggtcgagg gattgctcgt ggaggagcat
      781 atcaactttg gtcatctcaa ggccatcatc aaggaatacc tcgaacagat ttttgaaaaa
      841 cagctgacct tccggtttcg ccccagtttt ttccccttca ccgaaccctc ggcagaggtc
      901 gatatgagtt gcgtgatctg ttcaggcaag ggctgtcgcg tgtgcaagca gacgggttgg
      961 ctcgagatca tgggttgcgg catggtagac ccggcggttt tcgacgcggt caaaatcgat
     1021 cacagaaaat attccggtta cgcctttggg gtggggctcg atcgcctcgc catgctcaaa
     1081 tatggca
//
LOCUS       DATDUN010000644         1842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_490604, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000644 DATDUN010000000
VERSION     DATDUN010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490604"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1836)
                     /gene="guaA"
                     /locus_tag="VJC18_09020"
                     /pseudo
     CDS             complement(313..1836)
                     /gene="guaA"
                     /locus_tag="VJC18_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
BASE COUNT          451 a          474 c          427 g          490 t
ORIGIN      
        1 cttgttcgat cagctgttga cgcaactggg caaccgaaat cttttgttcc tcacgcccat
       61 aaacgacctc atcctgcgta agcgtgggcg ttgtctgtgg ctctgtgggt gttgttgtca
      121 cgggttttgt cataatatca cctcatccta tcgaatggga tagacatttg atagacattt
      181 tgtagacaag cacaatccat gccaacacag caaaatttaa catgtgtatt tttatgtggt
      241 tatgagacgc tatctggaaa tagggggagc ccatatgcca aaaacgtgtc aatttctcaa
      301 attgaggggc cttcactccc actcaatggt ggcggggggc ttggaggaaa tgtcgtagac
      361 cacacggttg atgcccttga cctcgttgat gatgcgcgtg gatattttgg agagcaaatc
      421 ctgtggcaaa tggacaaaat cggcggtcat gccatccaca gagtccacac aacgaagcgc
      481 cgccacattt tcatacgtgc gctcgtcacc catcacaccc accgatttga cgggaagcag
      541 aacaaccaac gcctgccaga tttttgacta ccaccctgct tttttaattt cgtcgatggc
      601 aatgtgatcg gcaagttgta aaatcctaac tttctctttt gtcaccgcgc ccaatattct
      661 cacgcccaac cccggtcccg gaaagggatg ccgcatgatg atctcatcgg gcatgccgag
      721 ttgtctgccc aattcacgca cttcgtcctt aaagagttca cgcaaaggct cgatgagttt
      781 gagtcccatt tttttgggaa gccctcctac attgtggtgt gatttgatca cagcagatgg
      841 ccccttgaag gattgcgatt caatcacatc gggatacaat gtgccctgag ccagatattt
      901 ggcccctttg atttttttgg cctcgcgcgt gaacacgtcg ataaaggttt tgccgatgat
      961 tttgcgtttt ctttcgggat cgctcacccc tttgagcgcc tttaagaatt catggctggc
     1021 atcgatgtat ttcaatttga tctggtaatg ctttttgaaa atcgtctgga catatttttc
     1081 ttctccaaat cgcaacaacc cgttgttgat aaaaaggcat gtcagttgtt tgccaatggc
     1141 ctgattgaga agcacagccg ccaccgatga atcaacgcca ccggagagtc ccaaaatcac
     1201 atgatccctg cccaccgtct cgcgaatatg ctggcattcg tatgagatga aatcgcgcat
     1261 gttccattgc ggctcaaccc cggcaatttt gaacaaaaag tttttgagaa tttgcatacc
     1321 acactttgtg tgatgcacct cgggatgaaa ttgaagcgcg tagattcgct tttcatcatt
     1381 cgcgatcgca gaaaccacct tcttgtctgt ctcccccata acccataacc cattgcccat
     1441 cacccctatg gaatccccat gactcatcca gattggggag tgatcttcga ccccttcaaa
     1501 gaggacatga ggttttacaa gctgtaaggt ggcataaccg tactcacgct gtttggccct
     1561 gatcaccttc ccacccatca gtttggtgat gagttgcatg ccgtaacaga tccccaaaat
     1621 ggggacaccc aaatcaaata ttttgggatc gatcgtcggc gccccgcgag acagcacact
     1681 ggcgggaccg ccggagagga cgatggcctg aggcttgaat tcgcagatgc tcgcgaaagg
     1741 ttccgcgcag gaacggattt cgcaatagac attcagctca cgcacacgcc gcgcgatgag
     1801 ttgtgtgtat tgagaaccaa aatcaatgat caggattttg tg
//
LOCUS       DATDUN010000645         1182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_284407, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000645 DATDUN010000000
VERSION     DATDUN010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284407"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..996)
                     /locus_tag="VJC18_09025"
     CDS             complement(358..996)
                     /locus_tag="VJC18_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HLD45565.1"
                     /translation="MPHTLEDIYQKLLSHFGPQHWWPGDTPFEICVGAILTQNTAWSN
                     VEKAIANLKNNQLLSFERLLATPAQSVAELIKPAGYFNIKTKRLKSFLGAIKREQGDF
                     DTLSSLSTSSLREFLLSISGIGPETADSMMLYAFERSVFVVDAYTNRMLTRHSLVDEE
                     ADYFRLQEYFEDHLEKDVALFNEYHALIVKLGKEFCRKSNPKCEECPLREEQ"
BASE COUNT          281 a          265 c          316 g          320 t
ORIGIN      
        1 atctacgctg attccatgag tcagggggct gatttttatc acttccaacg cagtctggag
       61 gcctatcaga agtccatcga caagaactcc acgatcatca tggggaccga ttccgagttc
      121 ttcaaggtct tcaagtcggg agggcagtag ggtaacgaac ttatcaccgc agagcgggat
      181 cccgttggcg ggacgagcgc acgaactcgc gaacgtacgt ctgctggcag gtaggtggca
      241 atccagaaga atttgtcatc cctgcgaagg cagggatcca gtagtaagat aaaaatggat
      301 tccagccttc gctggaatga caaatagttc gctattccat gttccttgtt cgatattcta
      361 ctgttcttca cgcagtggac attcttcgca tttgggattt gattttcggc aaaattcctt
      421 gcccagtttc acaataagcg catggtattc gttgaaaagg gccacgtctt tttccagatg
      481 atcttcaaaa tattcctgca gtctgaaata atcggcctct tcgtctacga gtgaatggcg
      541 ggtcaacatt ctattggtgt aggcgtccac aacaaacaca gagcgttcga acgcatacaa
      601 catcatgctg tcggccgttt ccggacctat gccagaaatc gacaacaaaa actccctgag
      661 ggaactcgtg gataatgacg agagggtgtc aaagtcaccc tgttcccttt tgatggcacc
      721 cagaaaactt tttagccgtt tggttttgat gttgaaatag ccggcaggtt tgatgagttc
      781 ggcaaccgat tgtgcgggtg ttgcaagaag cctctcaaac gaaagcagtt ggttgttttt
      841 gaggttggcg atggccttct ccacattgct ccaggccgtg ttctgtgtga ggatggcccc
      901 cacgcagatt tcaaacggcg tgtcgcccgg ccaccaatgt tgtggtccga aatgggaaag
      961 caatttttga taaatgtctt cgagagtgtg tggcatttga aaactatttt ttgattttga
     1021 tcttaccggg ttgcacgtct tcggtgagcg tttcgacatc gtcggggttg tagcggggca
     1081 gggtgaactg ctgggtggcc cttctctcaa gatcgatgtt gttcaaggaa tcttccagag
     1141 cgatgaattc gggaaggctg tgggactcag aatgcaggta gt
//
LOCUS       DATDUN010000646         1700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_284418, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000646 DATDUN010000000
VERSION     DATDUN010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284418"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VJC18_09030"
     CDS             <1..695
                     /locus_tag="VJC18_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLD45566.1"
                     /translation="GIGFAIPINLAKQALEQLRSKGKVSRGWLGLTVKDISEAEAKLL
                     GVDVRAGVVVTDLVPQGPSQIAGLNVGDVVARVNDEPVTNAQTMPSLIAKYLPGTAVT
                     LQIIRQGKPETIQTVLGDLDDPNKTFVYPATTSIAPVLPKDIIGIDVRDIEKADKLGN
                     KSGVLITNIHKDSAAFNLGLLRSDVITKINDSAIGNVDGFRKMIDKVARGEPLTFTVW
                     RSGQERSFTLQK"
     gene            complement(730..>1700)
                     /locus_tag="VJC18_09035"
     CDS             complement(730..>1700)
                     /locus_tag="VJC18_09035"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLD45567.1"
                     /translation="EKRHQAIPVFSDVILHPAFAKTEISKEKKLMLESIKNRSDNPAQ
                     TAFSLLLKHLFRGHPYQLHPLGEKLSINALSLEQLKKYYQLIMTPQNLVIAVTGDVNT
                     QEILELLNDQLACLKKSAFHKINLSKPNRPHDHPVLFKNQKKEQTHVAIGFLSTSLYD
                     QDKYAMEILSNILSGQGGRLFLELRDKQSLAYTVSTTLIEGLETGFFGAYIATEPSKA
                     DTAIKGIFKELDQLRDTEISQTELDRAKNYIIGNQEIENQKISAIAMQLALNEIYGQG
                     MEEWYEFTNRIKKVTTAQIQQAAQKYLRPERSITAITGPMKCRGES"
BASE COUNT          381 a          399 c          465 g          455 t
ORIGIN      
        1 agggcatagg tttcgccatc cccatcaatc tggccaaaca ggcgctggaa caactcagat
       61 caaaaggaaa agtttctcgt ggttggctgg ggctgacggt caaggacatc agcgaggccg
      121 aagcaaaact gctgggtgtc gatgtgcggg caggcgttgt ggtgacagac ctggttcccc
      181 aaggcccttc tcaaattgcc gggctcaacg tcggtgatgt ggttgcgaga gtgaacgatg
      241 agcccgtgac caacgcgcag accatgccat cgctcatagc caagtatctg ccggggaccg
      301 ctgtgacact ccaaatcatc cgacaaggca aaccggaaac catccagact gtcttgggcg
      361 atctcgatga ccccaacaag acctttgttt accctgccac cacatccatt gcgccggttc
      421 tgccaaagga catcattggt atcgacgtga gagacatcga aaaggccgat aagcttggca
      481 acaagtcagg ggtcctgatc accaatattc acaaggattc agcggccttc aatttggggc
      541 tattgagatc tgatgtgatc accaaaatca atgacagtgc cattggtaac gtggatgggt
      601 tcagaaagat gatcgacaaa gttgcccgag gggaacccct cacgttcaca gtttggcgga
      661 gtggacagga acgttctttc accctgcaga agtaggggcc gggctagacc ggcccaatga
      721 tcggacgagt catgactcgc ccctacactt catcggcccc gtaatcgccg tgatgcttcg
      781 ttccggacgc aaatatttct gtgccgcctg ctggatttgg gctgtggtga cttttttgat
      841 cctgttggtg aactcatacc attcttccat tccctgcccg taaatttcgt tgagggccag
      901 ttgcatggcg atggcgctga tcttctgatt ttcgatttcc tgatttccaa tgatgtaatt
      961 tttggcccga tcaagctcgg tttgcgaaat ttctgtgtca cgcaattgat ctagttcttt
     1021 gaagattccc ttgatggcgg tatctgcctt ggaaggttca gtagcgatgt aagcgccaaa
     1081 gaaccctgtt tccaatcctt cgatgagggt tgtggacact gtgtaggcca gagattgttt
     1141 gtccctgagt tccaaaaaca gccgcccccc ttgccccgaa aggatgttgc tgaggatttc
     1201 catcgcgtat ttgtcctggt cgtacaggga ggtactcaaa aaaccgatgg ccacatgcgt
     1261 ttgctccttt ttctggtttt taaacaggac gggatgatcg tgtggtctat tgggttttga
     1321 taaattgatt ttgtgaaagg ctgatttttt gaggcaggca agttggtcgt tgagcaattc
     1381 gaggatctcc tgcgtgttca cgtcgcctgt gactgcaatc acgaggtttt gcggggtcat
     1441 gatcagctga tagtatttct tgagctgttc caaactgagt gcgttgattg acagtttctc
     1501 acccaacggg tgcaattgat aagggtgtcc cctgaacaga tgcttgagca ggaggctgaa
     1561 ggccgtctgg gcgggattgt cggagcggtt tttgatgctt tccagcatga gttttttttc
     1621 tttgctgatt tctgttttag cgaaggcggg gtgcaaaatg acatcggaaa agacaggaat
     1681 ggcttgatgg cgtttttcac
//
LOCUS       DATDUN010000647         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_365807, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000647 DATDUN010000000
VERSION     DATDUN010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365807"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /gene="speA"
                     /locus_tag="VJC18_09040"
     CDS             complement(<1..1383)
                     /gene="speA"
                     /locus_tag="VJC18_09040"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HLD45568.1"
                     /translation="MKKPSWNKIKSDEVFNISGWGSPYFSINRHGDVAVHPRGKKNRS
                     FSLKILVDQIIERSIQTPFLLRFNDILLHRLACIKQAFETSISEYDYKGGHSIIYPIK
                     VNQQRHVVERIADFNQKNNNGLEAGSKPELLAIMALSRDPKTPIVCNGYKDEEYFEIA
                     LMGMKIGKQIYPVIEKYSEFETLIKVSKKLNINPEFGVRIKLSTKGSGRWQKSGGFRS
                     KFGLTINEVVKGVGVLKKAGLLDGFKLLHFHQGSQITNISTIKSSLKEAGRVYVELIK
                     LGVPLSILDVGGGLGVDYNGTGTNDDSSANYTLQEYANDIIFQVKEICDEAGIDHPTI
                     FTESGRAVAAHHSVLVFEVLGFSGYDRSDLPTKTKKAWTKPLKELFEIRQALKWEKDS
                     TRTMEHYHDSVSNFQECQNLFGLGYMNIEERGLAEEFYFSSLKRCLTILEKDEEYKNE
                     VADLEETLSDT"
     gene            complement(1380..>2192)
                     /locus_tag="VJC18_09045"
     CDS             complement(1380..>2192)
                     /locus_tag="VJC18_09045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HLD45569.1"
                     /translation="AAAIDFPIHNTDPSPSGSIVLDGQTVNFCIRQNKRLRRTRLLIS
                     PELGVIIESKKHISVSAAQQLVLCKQAWLVKNLEITNNRRERFVALKKLPNSTLVFGQ
                     EKIIVIKTGLPQQCVMETATQILLGFADHQITTTRLNKILSNWLKEKARNYLPTRLRH
                     LNRQFFPVHKSCVRDQKSLWGSCNSKHHLNLSWRLVMAPVHASDYILVHELCHTQHLN
                     HSKSFWALVQTLFPQYQKAEQWFKDYGFVLHSLPESAFLTVLGKQCGLKGVS"
BASE COUNT          493 a          479 c          589 g          631 t
ORIGIN      
        1 ggtgtcagac agtgtctctt ccaggtcggc cacttcgttt ttgtattctt cgtctttttc
       61 cagaatggtc agacagcgct tgagggaact gaaataaaac tcctccgcca ggccccgctc
      121 ctcgatgttc atgtagccga gtccaaacag attttgacat tcttgaaaat tagagacgga
      181 atcgtgataa tgttccatcg tgcgggtgct gtctttttcc cacttgaggg cttggcgaat
      241 ttcaaataat tccttgagtg gtttggtcca ggcttttttt gtttttgtgg gaaggtccga
      301 gcgatcataa ccggaaaacc ccaacacctc aaaaacgagc accgagtgat gtgccgcgac
      361 cgcgcgtccg ctttcggtaa agatggtggg gtgatcaata ccagcttcat cgcagatttc
      421 tttgacctga aagatgatgt cattggcgta ttcctgcaaa gtgtagttgg cggagctgtc
      481 atcattcgtg ccggttccgt tgtaatcaac gcccaaaccg ccgccgacat ctaaaatgga
      541 cagaggcaca cccagcttaa tcagctcgac gtacacgcgc cccgcttcct tgagcgaact
      601 cttgatagtg ctgatgttgg tgatttgcga gccttggtga aaatgcaata gtttgaagcc
      661 atcgagcagg cctgcttttt tcagcacgcc gacgcctttt acgacttcat tgatggtgag
      721 tccaaacttc gaacgaaacc cgccagactt ttgccagcgg cccgatcctt tcgtagagag
      781 cttgatgcgg acaccaaatt cgggattgat attgagtttt tttgatacct tgatcaaggt
      841 ttcgaattcc gaatattttt cgatgacagg gtatatctgc ttgccgatct tcatgcccat
      901 gagggcgatt tcaaaatatt cttcgtcttt gtagccgttg cacacaatgg gggttttggg
      961 atccctggaa agcgccatga tggccaggag ttcgggcttt gaacccgcct ccaggccgtt
     1021 gttgtttttt tgattaaagt ccgcaatgcg ttctactaca tgtctttgtt ggttgacctt
     1081 gatgggataa atgatggagt gtcctccttt gtaatcatat tcggagattg aagtttcgaa
     1141 ggcctgcttg atgcaggcca atctgtgaag caggatatca ttgaatctga gcaggaaagg
     1201 tgtttggatg cttctttcga taatctgatc gaccaatatt tttaggctga atgagcgatt
     1261 cttttttccc cgtgggtgaa cagccacgtc accgtgacgg ttgatcgaga aatagggtga
     1321 gccccagccc gaaatgttga aaacctcgtc cgattttatt ttgttccaag aaggtttttt
     1381 catgagacgc cctttagccc gcattgctta ccgagtacag tgagaaatgc gctctcaggc
     1441 agggaatgca acacaaaacc gtaatccttg aaccattgct ccgctttttg gtattgcgga
     1501 aagagtgttt gcaccagagc ccagaaggac tttgagtggt tgaggtgttg cgtgtgacac
     1561 aactcgtgga ccagaatgta gtcactggca tgaacggggg ccatgaccag tcgccaggac
     1621 aaattgagat ggtgttttga gttacaggat ccccacagag atttttggtc acgcacacag
     1681 cttttgtgga cagggaagaa ctgtcggttc aggtgacgga gcctggtggg gagatagttc
     1741 cgggcctttt ctttcaacca atttgagagg atcttgttga ggcgtgtggt tgtgatctgg
     1801 tgatcggcaa acccaagcag gatctgtgtc gctgtttcca tgacgcattg ctgtggcagg
     1861 cccgttttga tgacgatgat cttttcttgt ccgaagacga gggtgctgtt tgggagcttt
     1921 ttgagggcga caaaacgttc gcgtcgattg ttggtgattt cgaggttttt gactagccag
     1981 gcttgtttgc acaagacgag ttgttgtgcc gcgctgacgg aaatatgttt ctttgattcg
     2041 atgatcacgc caagttctgg tgagataagg agtcgtgttc tccgaaggcg tttgttttgc
     2101 cgaatgcaaa aattcactgt ttggccgtcc agcacgatag acccggaggg agaagggtct
     2161 gtgttgtgga tagggaaatc aatagccgcg gc
//
LOCUS       DATDUN010000648         4674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_284497, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000648 DATDUN010000000
VERSION     DATDUN010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284497"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VJC18_09050"
     CDS             <1..200
                     /locus_tag="VJC18_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45570.1"
                     /translation="FQLKGEGWYKDGYARPSEAPKKEDSKSTTSTEKTAPKESPDAKK
                     ETKNETKKDTKKETKVEPPKP"
     gene            complement(292..855)
                     /locus_tag="VJC18_09055"
     CDS             complement(292..855)
                     /locus_tag="VJC18_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HLD45571.1"
                     /translation="MLKTNQKIGILGSSFNPPHLGHLAVIEDLIKRDIFDQIWLIPVY
                     YHAFTKNLAPFEERLALVDLLIRELPQNKVQVLSTEKDLGNKPSYTIDVVTTLKLEYP
                     DCQFWLIVGSDIQKDLDKWHRIDDLKKIIQFHFIPRKGYEKSPYPEISSSEIRARLKK
                     GLPIDHLTTPEIVSYLKQKRVYDEGKA"
     gene            954..1577
                     /locus_tag="VJC18_09060"
     CDS             954..1577
                     /locus_tag="VJC18_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45572.1"
                     /translation="MRKLLLILAFTSFYIFVPFIYLNCSGQDLPYILGAQPSFSDEEK
                     LEECLKLDVLISSCVDVLQDYEESLKSQENTVASVAQTLDSFEFCQGEETLSDTTLTV
                     VKYQAQEEVVDEDVAATGDVETEGTEETTAADDVVQTEEETASATTCATIVTDMVTAY
                     QVCVSLSETDKFDDCYDVEHVMESAQGLCDTGDELVSQEFCTSLSSN"
     gene            1736..3187
                     /locus_tag="VJC18_09065"
     CDS             1736..3187
                     /locus_tag="VJC18_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HLD45573.1"
                     /translation="MPTLAVFNLDALVELIMVLIIKLAKYPRLLKILGLASRVSHYEP
                     GKPLRILIVGYNGARNTGADVRVEAMIRQFYHVLGKDNVHIGLLSIDPAHSKNYYHPP
                     TEIIRLDTVFFKDVLRACSEYHLAILSEGSCFKSKFANALTTLFMGAAGIMHAQNKPC
                     LAYGSETGKMDWLLTKFVMAYARSAYIISRTEPSYQATSKLGFEGGVGTDTAWMFEPS
                     PPDRGRELLMKQGWDGQQPVAGFAVINPFWWPVRPSIIKLLTMKLTGKGKENHYNRFY
                     FFTTSQERSDKFAAYISAVAVAAQEFCAKHKVFPIIIGMERLDMAANRILQEKLGRHV
                     PIYCSSDYNGYDLTAVLHQLSLLVTSRYHARVLSMTVGVPSIGISMDERLENLMTESG
                     HVADYYLSTDDVKLGEHLSEAMEMLWSHREQVKKEILATLPGYLEKMAGMADQLKGFI
                     ARWYPQFPLRPKPRDWRENLPALPGHLTRGIGV"
     gene            3184..3978
                     /locus_tag="VJC18_09070"
     CDS             3184..3978
                     /locus_tag="VJC18_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLD45574.1"
                     /translation="MKNYQDRVVFITGAAHGIGLSLAKLYDQAGAKVVACDVNPLSIS
                     EAQKALPRAVYYACDITKREAVYDLAKKVLATVGAPYILVNNAGIVENSNFLNCPDEK
                     LERTMQVNVLAHFWTLKAFLPEMIKNGEGHLCQVASAAGLLGVKGLAAYCASKHAVVG
                     FSNALRLELNDLTRGKIKVTVVCPGFINTGMFKGVKTALMTPLLDQNKIARIIFNAVA
                     KNKSFVLEPFMVKSVPLLNALLPTAAFNWVANVLKVNNAMDDIVKK"
     gene            4043..4462
                     /locus_tag="VJC18_09075"
     CDS             4043..4462
                     /locus_tag="VJC18_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45575.1"
                     /translation="MTDKGFTIVELVLIIILLGIMAVLATPRMISLRQEAREANRDFT
                     VSSIQEGLTLYNVQQVTLYEADVYPAALDSAPNGVCVKRANPTRECFSTVLRFGLADG
                     KWTRYFGGLLYRHNDTNTWYWYRSGIGFFMCIANCWE"
     gene            4467..>4674
                     /locus_tag="VJC18_09080"
     CDS             4467..>4674
                     /locus_tag="VJC18_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HLD45576.1"
                     /translation="MTPLNIVVLAAGLGKRMKTDKLKVLHELGGMTLVAHVVKNALGL
                     KPSKVIVVVPAEHESFKKIFARDVH"
BASE COUNT         1189 a         1071 c         1193 g         1221 t
ORIGIN      
        1 cgtttcaact gaaaggtgag gggtggtaca aggatggtta tgcccggccc tccgaggccc
       61 ccaaaaagga agattcaaaa tccactacat ctaccgagaa gactgcaccc aaagaatcgc
      121 ccgatgccaa aaaagaaacc aaaaatgaaa ccaagaaaga taccaagaaa gaaaccaagg
      181 tggaaccccc caagccttaa gcccccaagc ccctgccctg agcccctcgg ctacgctcgg
      241 ggtgaactca gcgaagggat ttattctgag cctgtcgaag aagtcgaagg gttacgcctt
      301 tccttcgtca tacacccttt tttgtttgag ataactgacg atttctggcg tggtcaggtg
      361 atcgatgggg agcccctttt ttaatcgtgc gcgaatttca gaacttgaaa tctcgggata
      421 gggtgatttt tcatagccct tccgggggat gaaatgaaac tggatgattt tcttgagatc
      481 gtcaatcctg tgccacttat ccagatcttt ttggatatcg gaccccacaa tgagccaaaa
      541 ctggcagtca ggatattcca gtttgagggt ggttacgacg tctatcgtgt aacttggttt
      601 attgccgagg tctttttcgg tgctaagtac ctggacctta ttttgaggca attccctgat
      661 caacaggtcc acaagtgcca aacgctcctc aaaaggggcc agatttttgg taaaagcgtg
      721 gtaatagact ggaatcaacc atatttggtc gaaaatatcc ctcttgatca aatcctcaat
      781 aactgccaga tgccccaaat gtggaggatt aaaggaggaa cccaaaatac cgattttttg
      841 attagttttt aacatatgat aacaacagtg aacaggagtc acacttgaat ttatttcgca
      901 taattcgcaa caatctaaca gttcgtgccg ataataaggg cgaaaggtta gatatgagaa
      961 aactactact tatcttagcg ttcacctcct tctacatctt cgtacctttt atttacctta
     1021 attgcagtgg tcaagaccta ccttacatct tgggagcgca gcccagtttt tcggatgaag
     1081 aaaaattgga agagtgtctc aagctcgatg tgttgatttc gagctgtgtg gatgtgttgc
     1141 aagactacga ggaaagcctc aaatcgcagg aaaacacggt ggccagcgtg gcgcagacac
     1201 tggattcatt cgaattttgt caaggagagg aaaccctgtc agacacaacg ctcactgttg
     1261 taaaatacca agcgcaggaa gaagtcgttg atgaagatgt agcggctaca ggtgatgtcg
     1321 agactgaggg caccgaagag acaacggctg ccgatgatgt tgttcagacc gaagaagaaa
     1381 ctgcctcagc gacgacctgt gccacgatcg tgaccgacat ggtaacggcc tatcaagtct
     1441 gtgtcagttt gagtgaaacc gataaattcg atgactgtta cgatgtggag catgtcatgg
     1501 aatctgccca agggctttgt gatacgggcg atgagcttgt gtcgcaggaa ttttgtacca
     1561 gcctgtcttc gaattaaggg cttctctaaa tcgccgttcc ccctctcatt atattcgtat
     1621 ctcaactgat aattccttga acgatggact cccagagcgt atagatgctc cctgttgttg
     1681 aaaaattatg ccttctattt tttttgccta acgcaattct gggggacgaa atatgatgcc
     1741 gacgttagct gtgttcaatc tggacgcctt ggtcgaactc atcatggtcc tcatcatcaa
     1801 actggccaaa taccctcgtc ttcttaaaat tttgggtttg gcctccaggg tcagtcatta
     1861 cgaacccggt aaaccgttac gcattttgat tgtgggctat aacggtgcac gcaataccgg
     1921 tgccgatgtg cgcgttgagg ccatgatccg gcagttttat cacgttttgg gcaaagacaa
     1981 cgtacacatt ggtttgcttt ccatcgatcc cgcacattcc aaaaattatt accaccctcc
     2041 cacagagatt attcgtctcg atactgtttt tttcaaagat gttctgcgtg cctgttcgga
     2101 ataccatctg gcgattctct ctgagggcag ttgtttcaaa tcaaagttcg ccaacgcgtt
     2161 gaccacgctc tttatggggg cggctggcat catgcacgcc caaaacaaac cttgcctcgc
     2221 ctatggttca gagacgggca aaatggactg gttgttgacc aaatttgtca tggcctacgc
     2281 acgctcggcc tacatcatct cacgcacaga gccgtcttat caggcgacat caaaattggg
     2341 ttttgaaggt ggcgtgggta cggacaccgc ctggatgttt gagccatcac cacctgatcg
     2401 tggacgagag ttgctcatga agcagggatg ggacgggcaa cagcctgtgg ctggctttgc
     2461 ggtcatcaat cctttctggt ggccggtcag accgagcatc attaaactgt taaccatgaa
     2521 gctcacgggc aagggcaaag agaatcatta caatcggttt tattttttta cgacatctca
     2581 ggaacgctct gataaatttg cggcctacat ttcggctgtg gctgttgcgg cccaggaatt
     2641 ttgtgccaaa cacaaggtgt ttcccatcat cattggcatg gaacgattgg atatggcggc
     2701 caatcgtatc ctgcaggaaa aactgggaag gcacgtgccc atctattgct cttccgatta
     2761 taacggttat gatttgacgg cggtgcttca tcagttgagt ttgctggtca cctcacgtta
     2821 tcatgcgcgg gtgttgtcca tgactgttgg cgtgccttcg attggcattt ccatggacga
     2881 acggcttgaa aacctcatga ccgaatctgg tcatgtggcg gactattatt tgagcactga
     2941 tgatgtcaaa ctgggtgaac atttgtctga ggcgatggag atgctttgga gccatcgtga
     3001 acaggttaaa aaagaaatct tggcaactct gccagggtat cttgaaaaaa tggcaggcat
     3061 ggcagaccag cttaagggct ttatcgcgcg ttggtacccg cagtttccgc tcaggcccaa
     3121 accgcgggac tggcgtgaga atctgcctgc gttgccgggg catttaacaa ggggaatagg
     3181 cgtatgaaaa attatcaaga ccgcgttgtt ttcatcactg gtgccgcgca tggcattggt
     3241 ttgtctttgg ccaagctcta tgatcaggcg ggtgccaagg tggttgcatg cgatgttaat
     3301 cctctctcca tcagcgaggc gcaaaaggcc ttgccacgag ccgtgtatta tgcgtgcgac
     3361 atcaccaagc gagaagccgt atatgacctg gccaagaagg tcttggctac ggtgggtgcc
     3421 ccctacatcc tggtcaacaa cgccggcatt gtcgaaaact caaattttct aaattgtccc
     3481 gacgaaaaac tggaacgcac tatgcaggtc aatgtcctgg cgcatttctg gacgctcaag
     3541 gccttcttgc cagaaatgat caaaaacgga gagggtcatc tctgtcaggt tgcctcggcg
     3601 gcaggactgt tgggtgttaa gggtttggcc gcttattgcg cctccaaaca cgctgtggtc
     3661 ggtttttcaa acgccttgag gttggaattg aacgacctca cccgaggcaa aatcaaagtc
     3721 acggttgtgt gtcccggttt tatcaatacg gggatgttta aaggggtcaa aacagcgttg
     3781 atgacgccac tgctcgatca aaataaaatt gccaggatca tctttaatgc tgttgccaaa
     3841 aataaatcgt ttgttctgga accctttatg gtcaagtccg tgccgttact gaacgctctg
     3901 cttcccactg cggcgttcaa ttgggtggcc aatgtgctca aggtcaacaa tgccatggat
     3961 gatattgtga aaaaatgaga gattgattat gctattgcaa gccggtattt tggcaacgac
     4021 gacgactcaa agaaaataaa acatgaccga taaaggtttt acaattgttg aacttgtttt
     4081 gatcatcatc ttgttgggca tcatggccgt attggccacg cccagaatga tcagtttaag
     4141 acaagaggcc cgtgaggcca accgcgattt cacagtcagc tcaatacagg aggggctgac
     4201 cctgtacaat gtgcaacagg taacccttta cgaagcggat gtgtaccctg ccgcgctgga
     4261 cagcgcgccg aatggggttt gtgtgaaaag ggccaatccc actcgtgagt gtttttctac
     4321 ggttcttaga ttcggcctgg cggatggcaa gtggacgcgt tattttgggg gactgttgta
     4381 tcgccacaac gataccaata catggtattg gtaccggtca gggataggtt ttttcatgtg
     4441 tatcgccaat tgctgggagt gaaaacatga caccactgaa cattgttgta ttggccgccg
     4501 ggctgggcaa gcggatgaaa acggacaagc tcaaggtgtt gcatgagttg ggcggcatga
     4561 cgctggttgc gcatgtggtg aagaacgccc tgggactcaa gcccagcaaa gtgattgtgg
     4621 ttgtgcctgc cgagcatgag tcgttcaaaa agatttttgc ccgtgatgtg cata
//
LOCUS       DATDUN010000649         1484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_490733, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000649 DATDUN010000000
VERSION     DATDUN010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490733"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..550)
                     /locus_tag="VJC18_09085"
     CDS             complement(239..550)
                     /locus_tag="VJC18_09085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HLD45577.1"
                     /translation="MKRGEVRWYRFQHPDKNRPVLILTRDSVMEYLNEVTVAPITSTV
                     RDIPSETHLSTEDGMPKECAVNCDHLQTVLKNKLGNVITTLPQFKMNEVQSAVRFALD
                     L"
     gene            complement(547..780)
                     /locus_tag="VJC18_09090"
     CDS             complement(547..780)
                     /locus_tag="VJC18_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HLD45578.1"
                     /translation="MRTIQMTLDNNLVKTVDRVVRETKTTRSAFTREALRLAIHGLKV
                     RQLEQKHRMGYAGKPIKKGEFDVWANEQAWGEQ"
     gene            complement(913..>1484)
                     /locus_tag="VJC18_09095"
     CDS             complement(913..>1484)
                     /locus_tag="VJC18_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45579.1"
                     /translation="LYAESTASGEDTYILHVEQIECDASHLQMQNIVTGRVPAVIMIA
                     HDQMVAFQDSKVVKFPDLELVPGQQREIDQQNPVMFSTRGVGMKISATISGVTNCIIP
                     NISTGINRTNTCVSLRYFFENVIGPLSTNETETIKTANPKLKDEKGPGFHSKNMHGDI
                     TLTLGQWIVLGGLPMGESRYMTCFVKVEK"
BASE COUNT          311 a          361 c          345 g          467 t
ORIGIN      
        1 atggttctct gcccgcaccg tggacaaaca attggctttt caaaatcggg acgttgggtc
       61 tgttcgttga tcccaatctt gccgacatcg caatcaaatt ccttgctaca cttctggcac
      121 tcaaagttga ttgacatatc attttccgtt ctctcaccat tctttacgcc aacgtcggcc
      181 ttcagcggcc gccggccttg cggccgtacg ctgaaaggca tggttggctt ttctgaattt
      241 acagatccag agcgaatcga acggctgatt ggacttcgtt cattttgaat tgaggaagcg
      301 tcgtgatgac gtttcctaat ttgtttttta aaacagtttg aagatggtcg caatttacag
      361 cgcattcttt aggcattcca tcttccgtgg atagatgggt ttcgcttggg atgtcccgca
      421 ctgttgatgt gatgggcgct accgtgactt cattcaaata ttccataacc gaatcgcgcg
      481 tcaaaatgag tacgggacga ttcttgtctg ggtgttgaaa cctataccat cggacctcac
      541 ctcttttcat tgttcacccc atgcctgctc gtttgcccag acgtcgaatt ctcccttttt
      601 aattggtttg ccggcatatc ccatccggtg tttttgttcc aattgccgca ctttcaaccc
      661 gtggatcgcc aagcggaggg cttcgcgggt aaaggccgat cgggtggttt ttgtttctcg
      721 gacaacgcgg tcaactgttt taacgagatt attgtcaagg gtcatttgta tggttctcat
      781 aacgatattc ctttatattg gtgtttatag ctataatacc catcattgat tcgttgtcaa
      841 tggcctgttt tccttttgag acaatgggcc tgatgagcgt gatgcaaccc gacaacgtcg
      901 gtttgctggc ctctattttt cgaccttcac aaaacatgtc atgtaccggc tttctcccat
      961 gggaagacca cccaaaacga tccactgtcc gagagtcagc gttatgtcgc catgcatgtt
     1021 ttttgagtgg aagcctgggc ctttctcgtc tttgagcttg ggattggcgg tctttatcgt
     1081 ttccgtctcg ttcgtcgaaa gaggccctat tacattctcg aagaaatacc tgagagaaac
     1141 acaggtattt gtccgattaa ttccggttga tatgtttggg attatgcaat ttgtcactcc
     1201 tgaaatggtg gcgctgatct tcatgccaac acctcgggta ctgaacatca cggggttttg
     1261 ctggtctatt tccctttgct gtcccggaac caattcaaga tccgggaact tgaccacttt
     1321 tgagtcttgg aatgcaacca tctggtcgtg ggcgatcata atgactgccg ggactctgcc
     1381 ggtcacaata ttttgcattt gcaagtgcga tgcgtcgcac tcaatctgtt ccacatggag
     1441 gatatacgta tcttctccgg atgccgtgct ttccgcgtat agac
//
LOCUS       DATDUN010000650         2552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_284592, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000650 DATDUN010000000
VERSION     DATDUN010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284592"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VJC18_09100"
     CDS             <1..390
                     /locus_tag="VJC18_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45580.1"
                     /translation="KASLMASIYNGGIGRFTSALKAHGANYLKGMSVQTQDYVERVTD
                     YCRAYQQGDQLYTTTYAIEEVEYLVDLLAMTYSDAGDVDWDGLKQDIRTNFFSSQEEI
                     TLVDDVKALIYYISTYDTSLAPEWPIM"
     gene            601..1866
                     /locus_tag="VJC18_09105"
     CDS             601..1866
                     /locus_tag="VJC18_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45581.1"
                     /translation="MKSIFRYGTFWFVFGVLVLVSVFLIQTNVLAREDGYTVVSESQK
                     GCLGADADYEWGEDGQCYRHEEATVMWLGFDHSWKKNPHRLNRLGSLYKKIQYEENAD
                     TPGGLLSAKRRSVFEVGAYDDEGVVRIKGEAVKSGVLGFYDGFVDEDCLVVSGEVGSA
                     DEVECPVFVDLQEAGLSGYDQVSVILRGFTLESESYDKGYNARGFTIRVIPVSRAGDE
                     YFFRAKFMLHAEHAPDRPSFDDACGQDNHCTHYQYSARIFYTLVGVNGVEGNLVEPSD
                     EGTNSYAQNITMYPFKTVPWTESAVRSAHIYGNTGFDQALVAIQGLSWHLNKWPHTEK
                     DGRYIRELKFYVAGRQYDAKTGEMAFLTNMYFSNTGAWPYGFEAHFTMWNTLVQFNDP
                     QGVASGVQWDKCVLEGGEAACTEDLLFEL"
     gene            1946..2242
                     /gene="yhbY"
                     /locus_tag="VJC18_09110"
     CDS             1946..2242
                     /gene="yhbY"
                     /locus_tag="VJC18_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HLD45582.1"
                     /translation="MSTLTTSQKKKLRGLAHHLEPIVFVGQKGFGEQLVLATEQALFD
                     HELIKVKFVEHKDEKKELAQKIAEQTNSTVVGMIGNIAMLYKENPEKKKNKIVV"
     gene            2239..>2552
                     /gene="cutA"
                     /locus_tag="VJC18_09115"
     CDS             2239..>2552
                     /gene="cutA"
                     /locus_tag="VJC18_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HLD45583.1"
                     /translation="MKHYLVYITAPHKEVAKTIGRTLVEDRLAACVNIIENMTSLYWW
                     EGKINEDSEVVLLAKTRESLVESLIAKTKILHPYECPCVVALPIEGGNQDFFAWMNKC
                     LS"
BASE COUNT          644 a          528 c          711 g          669 t
ORIGIN      
        1 aaggcctcgt tgatggcctc aatttacaat ggagggatcg gaaggttcac cagcgccctc
       61 aaggcccatg gggcaaatta tctcaagggc atgtctgtcc aaacccagga ttatgtcgaa
      121 cgggtaacgg actattgccg cgcctatcag cagggcgatc aattgtatac gaccacatac
      181 gccatcgaag aagttgaata tcttgtggat ctgttggcca tgacctacag cgacgccggc
      241 gatgtggatt gggatgggct caagcaggat attcgcacca actttttttc ctctcaagaa
      301 gaaatcacac tggtcgacga tgtgaaggcc ctcatctatt acatttctac ctacgacact
      361 tccctggccc ccgaatggcc aatcatgtag ctctttaggg ggctctttag aggggcccat
      421 tagactatta cgacaacgga ttctcgtttt tggcaatccc cgccaaggat tatcaaaaca
      481 gccgttcatt cctttcttaa aaaatcaacc aaaacaacgc gatgcctttt tcgtcgatgg
      541 tatgggactt gctagttaca taaatagcaa gggggtttaa gttttttgag ggcaccgttt
      601 atgaaaagca tctttcgtta tggcaccttt tggtttgtgt ttggcgtcct ggttttggtc
      661 agcgtttttt tgattcagac caatgtattg gccagggaag atggctatac cgttgtttct
      721 gaatctcaaa agggatgcct gggtgccgat gctgactatg agtggggaga ggatggccaa
      781 tgttatcgcc acgaagaggc gacggtgatg tggttgggtt ttgatcattc ttggaaaaaa
      841 aatcctcatc gcctcaaccg gctggggtcg ttgtacaaaa aaattcagta tgaggaaaat
      901 gccgatacgc ccggggggct gttgtctgca aagagacgct ctgtgtttga ggtgggggct
      961 tatgatgacg aaggtgttgt gcgcataaag ggcgaagcgg tcaagagcgg tgttttgggt
     1021 ttttacgacg ggtttgttga cgaggactgc ttggttgttt ctggtgaggt gggaagcgcg
     1081 gacgaggtgg aatgccctgt gtttgttgac ctgcaagaag cggggctgtc tggttatgat
     1141 caggtgtctg tgatcttaag agggttcact cttgaatccg aatcatacga taagggatac
     1201 aacgccagag ggtttacgat cagggttatt cctgtttcgc gtgctggtga cgaatatttt
     1261 tttcgtgcca agttcatgtt gcacgccgaa cacgctcccg atcggcccag ttttgatgat
     1321 gcgtgtggac aagataatca ttgcacacat tatcaataca gtgctcggat tttttacaca
     1381 ctggtggggg tgaacggggt cgaggggaac cttgtggagc catctgatga ggggaccaac
     1441 tcgtacgcgc aaaacatcac aatgtatcca ttcaaaactg ttccctggac tgagtccgct
     1501 gtgcgaagtg cccacattta tggcaacacg ggatttgatc aggccctggt ggctattcag
     1561 gggctgagct ggcacttgaa taaatggccg catacggaga aagatggtcg ctacatccgt
     1621 gagctcaagt tctatgttgc gggaaggcaa tacgatgcca agacaggaga gatggcgttt
     1681 ttgacgaaca tgtatttttc gaacacgggt gcctggccct atgggttcga ggcgcatttt
     1741 acgatgtgga atacactggt tcagttcaac gatccgcagg gggttgcctc gggggtacaa
     1801 tgggataagt gtgtgctgga aggtggcgag gcggcctgca cggaggatct gttgtttgaa
     1861 ctttagcacg cttgggtggg aggcaagaca gcagtcgtgg taaattccat tgtattcttt
     1921 attattcctg tttattaaat acggcatgag cacactgaca acgagtcaaa aaaagaaact
     1981 cagggggttg gcacaccatt tggaaccgat tgtttttgtg gggcaaaagg ggtttggtga
     2041 acaacttgtt ttggccacag agcaggcctt gttcgatcac gaactgatca aggtcaagtt
     2101 tgttgaacac aaggatgaaa aaaaagagct cgctcaaaaa atcgccgagc aaaccaatag
     2161 tactgttgtg gggatgatcg ggaacattgc catgctgtac aaggaaaatc ccgaaaagaa
     2221 gaaaaataaa attgtggtat gaaacactac cttgtttaca tcaccgcgcc tcacaaagaa
     2281 gttgcaaaaa caatcggacg gactctcgtc gaagatcgcc tggcggcctg tgttaacatc
     2341 attgagaata tgacctcttt gtactggtgg gaaggcaaga tcaatgagga cagcgaagtt
     2401 gtcttgttgg ccaaaacgcg ggaatccctg gttgagtcct tgattgcgaa aacaaaaatc
     2461 cttcatccct acgagtgtcc ctgcgtggtg gcgctcccca tcgaaggtgg caatcaggat
     2521 ttttttgcct ggatgaacaa gtgtttatcc ga
//
LOCUS       DATDUN010000651         2357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_35662, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000651 DATDUN010000000
VERSION     DATDUN010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35662"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..517)
                     /locus_tag="VJC18_09120"
     CDS             complement(20..517)
                     /locus_tag="VJC18_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45584.1"
                     /translation="MNRAVLLIILCVGFCVACGTNTVPSDMAGIAPPSATGQSKTSEK
                     RAMIDGTYYYDLSANKNYQLGLKDSLFEGVKPTEIDNILKIFRPFHVEINGDTAIVSF
                     SREVISARLEKISSSAEETKFKLLPLDAKHKDQEAITTINKNQLMIDPGKQEKDKMFF
                     TKAPE"
     gene            complement(576..1526)
                     /locus_tag="VJC18_09125"
     CDS             complement(576..1526)
                     /locus_tag="VJC18_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45585.1"
                     /translation="MSFDYADVTGDHVVDIVYVTRHELIVGQFEEKAISQVATKSFGL
                     KTTLHRVNLGDWDQDGVIEMLINGFVRGRAITWLYRVEGGLFVLEQEFDKLILPLQFA
                     RGTGLYVQAQQSGWEWSDRLGKMVIKNGKWEEESTSYQLQKGIGGGTLPLFSYAAFGN
                     DLVAMTSEQRIWHSDTLGKKLWKSPMSYGSAVDYVDVAEKDILGMQRAGRFFIAPRLS
                     VNDHRLLLIKNDGYLNSLLGTMPDIKSSELVLLDKTGVGFQEKKVSTRFAGIITDAKF
                     IDFDGDGSAEVMISFLPKKGGYLEGYVGYEGMMAVLPMPQ"
     gene            1794..2345
                     /locus_tag="VJC18_09130"
     CDS             1794..2345
                     /locus_tag="VJC18_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45586.1"
                     /translation="MSTGGNYSNTTSGYTVDRQTYSYDTTSGDMNTMPQIGDFGGTSQ
                     GEHHATGYQSWMDSMTSLQAAVVDAQSAYDSSAVEAEAAVEASYLDAEARKVEANLAM
                     ETSIHKDETDIEIAQMEADIEWEKLAVERDKIEQVDAVNAQANMRASDALFMEAEAEM
                     EEAEAERDEVKYDYMGGDDSSVW"
BASE COUNT          645 a          629 c          510 g          573 t
ORIGIN      
        1 caactcattc cggagctttt cattccggag ctttggtaaa aaacatcttg tctttttcct
       61 gcttgcccgg atcgatcatc aactgatttt tatttattgt ggtgatcgcc tcctgatcct
      121 tatgtttggc gtcgagaggg agtaacttga attttgtttc ctcggcagac gatgagattt
      181 tctccaaacg ggccgaaatc acttcacgcg aaaaactcac aatggccgta tcgccattaa
      241 tttctacatg aaagggacga aagattttca gtatgttgtc aatctccgtc ggttttaccc
      301 cctcaaacaa agaatccttg agtcccaact gatagttttt gttggcactc aaatcgtaat
      361 agtacgtccc atcaatcatt gccctttttt cggaggtttt gctttgcccg gtggcagagg
      421 gaggcgcgat ccccgccata tccgaaggca cggtattggt tccacaggcc acgcagaaac
      481 caacacataa gatgatcagt aaaacagctc tgttcatata gtctcccctt atgccatacg
      541 ccttttgcac tagccaaagg ttcaacgcaa gcgttttatt gcggcattgg caaaacggcc
      601 atcatgccct cataccccac gtacccttcc agatagccac cttttttggg caaaaaagaa
      661 atcatgacct cagccgagcc atccccatcg aaatcaatga acttggcatc ggtgatgatg
      721 cccgcaaacc gtgttgacac ctttttttcc tggaaaccca caccagtctt atccagcagt
      781 accagttccg atgatttgat atcgggcatg gtccccaaca gggaattcaa ataaccatcg
      841 tttttgatca ggagcaaacg atggtcattc acggatagcc tgggggcaat gaaaaatcgc
      901 cctgcgcgtt gcatacccaa aatatccttc tcggctacgt ccacataatc aaccgccgac
      961 ccataactca tggggctttt ccacagtttt tttcccaaag tatcactgtg ccaaattcgt
     1021 tgttcactgg tcatggccac cagatcatta ccaaacgcgg cgtaggaaaa taagggcagg
     1081 gtcccaccac caatcccttt ttgcagttga tacgacgtgc tctcttcttc ccattttcca
     1141 tttttaataa ccatttttcc cagtcgatca gaccattccc aacccgactg ttgcgcctgc
     1201 acgtacaaac cggtgcctct tgcaaattga agtggcaaaa tcagtttatc gaactcctgt
     1261 tccaaaacaa atagcccacc ctctacccgg tagagccagg tgatcgccct gcccctgaca
     1321 aacccgttga tgagcatttc aatgactccg tcctgatccc aatcacccag attgacacgg
     1381 tgcagagtgg ttttgagacc aaacgatttg gtcgcaacct gactgatcgc tttttcctca
     1441 aattgtccaa cgatcaattc gtgccgcgtg acatagacaa tatcaacgac gtgatcacca
     1501 gtcacatcgg cataatcaaa gctcatgatc tcgcgaggcg tgatccccga ctcccaaagg
     1561 ctgtctgtaa aaaccggggc agaccaaagc ttcgttgggg tgaacaagac aacgacaaac
     1621 aatatgacac aacacacgct tctatgaggc atcggtccct acccttcttc aataaccttc
     1681 tcagagttaa tgtttgtata tatgaaggtt atttgataaa atataaaaaa agcgtacaaa
     1741 acacaactct gacgtagcga tcgtacgata atagtcttag gaaggagaat tttatgagta
     1801 caggcggcaa ttattcaaac acgaccagtg gttacaccgt agatcgacaa acctattctt
     1861 acgacacgac aagcggtgat atgaacacca tgccccaaat aggtgatttt ggcgggacta
     1921 gtcaaggcga acaccatgca actggctatc agtcttggat ggacagcatg acctccctgc
     1981 aggcggcagt ggtcgacgct caaagcgcct atgactcctc ggccgtcgag gccgaagcgg
     2041 ccgtcgaggc atcgtacctc gatgccgagg cccgcaaagt tgaggccaat ttggcgatgg
     2101 aaacctccat acacaaagac gaaaccgaca tcgagattgc ccaaatggaa gccgatatcg
     2161 aatgggaaaa actcgctgta gaacgcgata aaatcgaaca ggtcgatgcc gtcaacgccc
     2221 aagccaacat gcgcgcctcc gacgccctct ttatggaggc cgaggccgaa atggaagagg
     2281 cagaggcaga gcgagacgaa gtcaaatacg attacatggg aggtgatgac tcctcggtct
     2341 ggtaatggtt cggcgtt
//
LOCUS       DATDUN010000652         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_402300, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000652 DATDUN010000000
VERSION     DATDUN010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402300"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VJC18_09135"
     CDS             complement(<1..669)
                     /locus_tag="VJC18_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLD45587.1"
                     /translation="MSSKKYFWLISFVLYLTHSTFAHAFSVNIGKPDTFSKIAKEVGP
                     TVVNIYTTKDLRESPNSYSGVNPYFEKNYGAVYKKFQRNQTTGSAYHSLGTGFIITAE
                     GTVLTNYHVIAGADDIYLALNDGQKVEASLLGVDTTLDIALLKINKVDTYPVAELGDS
                     NNTVIGDWVVAVGNPFGLGQTITAGIVSAKGRALGAGPYDDFIQTDASINPGNSGGPL
                     FDMDG"
     gene            997..2001
                     /locus_tag="VJC18_09140"
     CDS             997..2001
                     /locus_tag="VJC18_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45588.1"
                     /translation="MRTYRLIYLSLLLCLFVPYAQAFTLAETGPTALTCPKVASAVII
                     SCAYDSDLTAINCSDRAATPRITVAIKFRGATEATKFDIKGISRGAFALMVNSNADAN
                     PGIITPADVNHFLFDGRSRTLYLLQDEADINLLISRIDAYADFLKAHSVDDGIYGVCV
                     PNDAEIPIFIDPATAIAGLPKYLAEEPTDAVAGEGEAEVEAEVEEEVEAEVEGEGEAE
                     VEGEGEVEGEGEVVIEGGDEVAVDVIDTDTDGIPDATDNCAQIANEDQLDADKDGIGD
                     DCEVAVDANKVFANSDDGGCSLNPLATTSNHAPYAMILSVLFGLVLMRRVPSLKINNQ
                     "
     gene            2144..>2571
                     /locus_tag="VJC18_09145"
     CDS             2144..>2571
                     /locus_tag="VJC18_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD45589.1"
                     /translation="MIKRQISHKIIEFIKCNQEHKNVLLVDGVRQVGKTTAIKSAITS
                     SQQQCVELNLEKKKILVEKIDRTKEFSDFEELLTQECQFTPNTGKILFIDEANESLRL
                     GHYVRQMKEDWPRQTVILSGSMMTRLFRDTNCRIPVGRFET"
BASE COUNT          734 a          589 c          584 g          664 t
ORIGIN      
        1 cccatccata tcaaataacg gtccgccgga attgccggga ttgatcgagg catcggtctg
       61 gatgaaatca tcgtacggtc cggcacccaa cgcgcgcccc ttggccgaca caatgcccgc
      121 tgtgatggtt tgtccgagtc caaacgggtt acccacagcc accacccaat caccaatcac
      181 ggtgttgttg gaatcaccca actctgccac gggatacgta tcaaccttat tgattttgag
      241 caaagcgata tcgagggttg tgtccacccc cagcaatgag gcctccactt tttggccatc
      301 gttaagagcc aggtaaatgt catcagcccc cgcgatcacg tgataattgg tgagcacggt
      361 gccctctgcc gtaatgatga accctgtgcc cagcgaatga taggcacttc ccgttgtttg
      421 gttgcgctga aattttttat aaacagcacc gtagtttttt tcgaaataag ggttcacgcc
      481 actgtacgag ttgggcgatt cgcgaaggtc cttggtcgtg tagatattga cgacggtggg
      541 accgacctct ttggcgattt tggaaaacgt atcgggcttg ccgatgttga cggaaaacgc
      601 gtgtgcgaaa gttgagtgcg tcaggtacaa aacaaaagaa atcagccaaa aatatttttt
      661 tgaactcata ggcgttctca tagaaaacaa tcgcattttc cgcaactgac acatacaatt
      721 gaaatgtttt ccccatcatt gcgagtcgta ggggccgggc tggaccggcc cccgatcata
      781 cgcccctcga atatgaactg gaaataattg cccattcaaa atgataaaat atcaaaatag
      841 taacatcaat cccccaacat tattttgtta acctgttaat tttacaaata attatcatta
      901 atcaatttgg caccagtttt gctcattagg ccttcgaaca accttaatag tccgcaattg
      961 cccagtaaaa gagcatagcg tgacaaggag aaacaaatga gaacctatcg actgatttat
     1021 ttgtcattat tgctgtgcct ttttgttccc tacgcccaag cgtttacact agctgaaact
     1081 ggacccactg cgctgacttg ccctaaagtt gcctctgcgg tgataattag ttgcgcttac
     1141 gattcagact tgacggctat taactgttct gatcgcgcgg ctaccccaag aataactgtt
     1201 gcaataaaat ttaggggggc aacagaggct accaaatttg acattaaggg aatttctcgt
     1261 ggcgcatttg ccctcatggt aaacagcaat gccgatgcaa atcccggaat aatcacgcca
     1321 gcagacgtca accattttct atttgatggt cgctcacgga cactgtatct cttacaagac
     1381 gaagctgaca tcaatttgct aatctccaga atcgatgcgt atgctgattt tctaaaagct
     1441 cattctgttg acgatggtat ctatggtgtt tgcgtaccaa atgacgcgga gattccaatc
     1501 tttattgacc ctgccactgc tatagctggt ctccccaaat accttgctga agaacccaca
     1561 gacgccgttg ccggtgaagg tgaagctgaa gtcgaagcag aagttgaaga agaagtcgaa
     1621 gctgaagtcg aaggtgaagg tgaagccgaa gtcgaaggtg aaggtgaagt cgaaggtgaa
     1681 ggtgaagtgg tcatcgaggg tggagatgag gtagcagtcg atgtcattga cacagatacc
     1741 gatggtatcc ccgacgcaac agacaactgc gcacaaatcg ccaatgagga tcagcttgac
     1801 gcagataagg atggcattgg tgatgactgc gaagtagccg tggatgccaa caaggttttt
     1861 gccaattctg acgatggtgg ttgttcactc aacccactcg ccacaacctc aaatcacgcc
     1921 ccgtatgcca tgatattaag cgtgctgttt ggacttgttt tgatgagacg agtgccctct
     1981 ttgaaaataa acaatcagtg attccccttt gatcccagaa tagtccccaa aaggcctcca
     2041 aacggaggcc tttttttctc cgtcgtttgg cgcaccttct caaatatgac catatttcat
     2101 gattatttgt tgctaatacg gccatatttt agtacgataa aacatgatca aaagacaaat
     2161 atctcataaa ataatcgaat ttatcaaatg taatcaggag cataaaaacg ttctccttgt
     2221 tgatggcgtc agacaagtag gcaaaacaac tgcaataaag agtgctatta ctagttctca
     2281 acaacaatgc gtggaattga atctggaaaa aaagaaaata ctcgtagaaa aaatagatcg
     2341 cacaaaggaa ttttctgatt ttgaggaatt gttaacacag gaatgccagt ttactccaaa
     2401 tacaggcaaa atcctcttta ttgatgaggc aaacgaaagt ctgcgtttgg gccattatgt
     2461 tcgtcaaatg aaggaggatt ggccccggca aacagtcatt ttgagcgggt ccatgatgac
     2521 acggcttttt cgcgatacga attgccggat ccccgtgggg cgtttcgaaa c
//
LOCUS       DATDUN010000653         1564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_527430, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000653 DATDUN010000000
VERSION     DATDUN010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527430"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VJC18_09150"
     CDS             complement(<1..549)
                     /locus_tag="VJC18_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45590.1"
                     /translation="MKTYRLITLSIFVCLFALYSQSATAVPPPPPAESSHTDLTCPTE
                     APARLISCSYNVTMKTIDCVDASGGFEKPFFVIGFPGATADFTFDIKGISRSAFALMV
                     NSNADGDTTKITPDDVDHFILDGQTLTLYLLQEEADIDFLIARIAAYEKFLSEASLSD
                     GLYGACVPDLSEYPIQIDPASAD"
     gene            complement(686..>1564)
                     /locus_tag="VJC18_09155"
     CDS             complement(686..>1564)
                     /locus_tag="VJC18_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HLD45591.1"
                     /translation="SSIRKYGLEEWVSRADKVTEISPLTHQTLLELADHYLICGGLPA
                     ITLHYLGHVARPDLNQKFTEYLATMKQDFLRFFSEEYVNLFDRAIVSVANILGQPYKK
                     TLMIQNNPKLAENILSVFEGWRFITKIEQRTHNPNNSNSFHPKRYLFDVGLAKFKREM
                     GVPSINIINTLNSAHREPLGGLLEQWACNELLPRFPDICGFREKNYEIDFIQKTHNQI
                     IPIECKAALKLNTHQYRGLDLYNRVYQNRNAVMISLAPYSRIQRTDYTIHIIPAYAVS
                     CLEELVANPATNAHSK"
BASE COUNT          409 a          304 c          406 g          445 t
ORIGIN      
        1 gtcggcggaa gcggggtcaa tttggattgg atactcgctc aggtctggca cacaggcacc
       61 atacaatcca tcgctcaaac ttgcctctga gagaaatttt tcataagcag cgatccgggc
      121 gatgagaaaa tcgatgtcgg cttcttcttg taaaagatac agtgtcagtg tctgaccatc
      181 caggatgaaa tggtcgacat catctggcgt gatttttgta gtatcaccat cagcattgct
      241 gtttaccatg agggcaaacg cgctacgaga aatgcccttg atgtcaaaag taaaatcggc
      301 agttgcccca ggaaaaccaa tcacaaagaa aggtttttcg aaccccccag aggcatcaac
      361 acagtcgatc gttttcatgg tgacgttgta agaacagctg atcaaacgag caggggcctc
      421 cgttggacaa gtcagatccg tgtgagagct ttcggctgga ggtggtggag gtacggccgt
      481 ggctgattga gaatagagtg caaacaaaca aacaaaaatc gatagtgtga tcagtctata
      541 ggttttcatg attcctcccc aaaaaagttg ttcatgagcc ctattatgca atatcggtgc
      601 cagtgtttgt ggcaataatt ttatataata acaaatggtt acaaaatatt gttaagaaat
      661 ggatatgtgc ttttttgatg ttttatcatt ttgaatgggc atttgttgcc gggttggcta
      721 ccaattcttc taaacaagac acggcgtagg cggggatgat gtggatggtg taatcagttc
      781 tttgaatgcg ggagtagggt gccagggaaa tcatcacggc attcctgttt tgataaactc
      841 ggttgtatag gtccagaccc cgatactggt gtgtgttaag cttgagagcg gccttgcatt
      901 caatggggat gatctgattg tgagtctttt ggatgaaatc aatctcataa tttttttccc
      961 tgaaaccgca aatgtcgggg aagcgtggca aaagctcgtt acaagcccac tgctccaata
     1021 gtcctcccaa tggttcgcgg tgggcgctgt ttaaggtgtt gatgatgttg atggaaggga
     1081 cacccatttc gcgtttgaat tttgccagcc ccacatcgaa gaggtagcgt ttggggtgaa
     1141 aagagtttga gttgttggga ttgtgtgttc gctgttcgat ctttgtgatg aatctccagc
     1201 cttcgaagac ggacaaaata ttttctgcca attttgggtt gttctgaatc atcagcgttt
     1261 ttttataggg ttgccccaaa atgttggcga cgctgacgat ggcgcgatcg aacaaattga
     1321 catactcttc ggaaaaaaat cttaaaaaat cctgcttcat tgttgccagg tattccgtaa
     1381 atttttgatt gaggtctggt cgtgcaacat gcccaagata atggagtgtg attgcgggca
     1441 ggccgccaca gatgagataa tggtcagcaa gttccaggag agtttgatgc gttagtggag
     1501 aaatctctgt gactttgtcg gcgcgcgaga cccattcttc cagaccatat tttctgatcg
     1561 atga
//
LOCUS       DATDUN010000654         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_319554, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000654 DATDUN010000000
VERSION     DATDUN010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319554"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1067
                     /locus_tag="VJC18_09160"
     CDS             18..1067
                     /locus_tag="VJC18_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLD45592.1"
                     /translation="MTQYEIIKNQLLKNPCKWLVTGVAGFIGSNLLEALLCLNQEVVG
                     LDNFITGKKENLRAVKESVSAQQWQRFHFIEGDICDLAVCQRSASGVDYVLHQAALGS
                     VPRSIENPLRTNQINIDGTLNVFVAVRDQKVKRLVYASSSSVYGDHPGLPKVEHSVGT
                     PLSPYAVTKAVNELYAGVFASTYGLELIGLRYFNVFGKRQDPDGPYAAVIPKWIASLI
                     MGEPVVIYGDGETSRDFCFVENVVQANILAATTENREAVNQVYNVAVNQRTTLTELFG
                     ILRDVLATSLPHIKDSRPLYEDFRAGDIRHSLADISKAIRLLGYHPQFQIKEGIEKSL
                     GYYLGKCRGKTCSRV"
     gene            1302..1832
                     /locus_tag="VJC18_09165"
     CDS             1302..1832
                     /locus_tag="VJC18_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HLD45593.1"
                     /translation="MQMLDAIITSKSRVKVLLKFFLNPGVKAYLREMSTEFGDSTNSV
                     RVELNRLVEAKLLKIQKDGRNIYYSANMEHPLYPEINTIFRKVTGVDKIYEWISSIGN
                     LEQAYITGDYAKGIDGGIIDIILVGDIDKKSVFEMVERTEQFIGRKIRPLLLSQQEFV
                     NMNGKLANQRLLKLWG"
     gene            1840..>2308
                     /locus_tag="VJC18_09170"
     CDS             1840..>2308
                     /locus_tag="VJC18_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HLD45594.1"
                     /translation="MKNWYVINTKPQRENDVSQRLSGAGFEVCNPKVKDFNCRVKSLF
                     PNYIFTCCDFDESANYQLIKYTRGVNKVLGTKTNPVQIPTDVVLAIRVRLGPGEVLER
                     DRLVVGSQVKIKKGLLKDLIAIIEKPSTPDGRVAVLLKLYERELRTKVSVKELV"
BASE COUNT          644 a          456 c          579 g          629 t
ORIGIN      
        1 cagaaagcac gtaagaaatg acccaatacg aaatcataaa aaatcaatta ctcaaaaacc
       61 cctgcaaatg gctggtgacg ggggttgccg ggtttattgg ctctaatttg ctcgaagccc
      121 tcttgtgcct caatcaagag gtggttggtc tggataattt tattaccggt aaaaaagaaa
      181 acttgcgggc agttaaagag agtgtttctg cccagcagtg gcaacgattt cattttattg
      241 aaggtgatat ttgtgatctc gcagtgtgtc agaggtcggc gagcggtgtt gactatgtgt
      301 tgcatcaggc ggcactgggt tctgtcccac gttccattga aaaccccttg cgaacaaatc
      361 aaatcaatat cgatggcacg ctcaacgtgt ttgttgccgt gcgtgatcaa aaggtcaaac
      421 ggctggtcta tgccagcagt agctctgttt atggggatca cccgggattg ccaaaagttg
      481 aacacagcgt gggcacgccg ttatcgccgt acgcggtgac caaagctgtc aatgaactct
      541 atgccggtgt ttttgccagc acgtatgggc tggaactgat aggtttgcgg tattttaatg
      601 tgtttggcaa acgtcaggac cccgacggtc cttacgcggc agtgattccc aaatggatag
      661 cgtccctgat tatgggggag ccagtggtga tttacggcga tggcgaaaca agccgcgatt
      721 tttgctttgt tgaaaatgtg gtgcaggcca acattttggc cgccacgacc gagaatcgtg
      781 aggcggtcaa tcaggtgtat aacgtggctg tcaatcaacg gacaacgctc accgaattgt
      841 ttggaatttt aagagacgtc ttggccactt ctttgccaca catcaaggac agtcgtcccc
      901 tttatgagga ttttcgtgcg ggcgatatca ggcactctct agcggatatt tcaaaggcca
      961 ttaggctact gggctatcat ccgcaatttc aaatcaaaga ggggattgaa aagagtttgg
     1021 ggtattattt gggaaaatgt agaggaaaaa cgtgttcgcg tgtttaagtg ttcatgtgtt
     1081 caagagggaa aaaaacgcaa ggcgcaacaa gtgaatgaca aatttctgaa tagacacttt
     1141 tgtgtgattt gttcaactgg actgtatttt ctatttccaa tcattctgat aatttcttag
     1201 tttacggtat gttgctcaat gtattgcaaa gttgtttcta attattcaaa tcttgacaat
     1261 ctacccctgt ttgctatgag cgagtaaatt aattactcat aatgcaaatg ttagacgcca
     1321 tcatcacatc caagtcgcgt gttaaggtct tgctcaagtt ttttttaaac ccaggggtaa
     1381 aggcctattt gcgcgaaatg tccaccgagt ttggggattc cacaaactca gttcgtgtgg
     1441 aactcaatcg tttggtcgaa gccaaactgc tcaaaattca aaaagacgga cgcaatattt
     1501 attactcggc caatatggaa caccccttgt atccagaaat caacacgatc ttcagaaaag
     1561 tcacgggtgt cgacaaaatc tatgaatgga tttcatccat tggaaatctg gaacaggcat
     1621 acattactgg tgattatgcc aaagggatcg atggcggcat tatcgatatc attttggtgg
     1681 gagatatcga taaaaaatct gttttcgaaa tggtggagcg tacggaacaa ttcattggca
     1741 gaaagatcag accgctctta ctgagccagc aagaattcgt gaacatgaat ggcaagcttg
     1801 ccaatcagag gcttctaaaa ttatggggat aaagcacaca tgaagaactg gtatgtcata
     1861 aatacaaaac ctcagagaga aaatgatgtt tcgcaaagac tctcaggtgc cggttttgag
     1921 gtgtgcaacc ccaaagtgaa ggatttcaac tgccgtgtga aatccctgtt tcccaattat
     1981 atttttacat gttgtgattt tgatgaatcc gcgaattatc aactcatcaa gtacacccgc
     2041 ggggtgaata aagtcttggg taccaagaca aatccagtac agattccaac tgatgttgtt
     2101 ttggccatca gagtgcggct cggccccggt gaggttctgg aacgtgatcg gctggttgtt
     2161 ggcagtcagg tcaaaatcaa aaagggtttg ctcaaggatt tgattgcgat catcgaaaaa
     2221 ccatcaacgc ccgatggacg ggtggcggta ttgcttaagt tgtatgagag ggaactgcgg
     2281 acgaaggtga gtgtgaagga gttggtgg
//
LOCUS       DATDUN010000655         1224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_74921, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000655 DATDUN010000000
VERSION     DATDUN010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74921"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VJC18_09175"
     CDS             complement(<1..988)
                     /locus_tag="VJC18_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45595.1"
                     /translation="MNKIIFLLSCFFCVFLTAEAAAQTATPVAAGSIMDANDLVLDLS
                     LRDGNALIKPLLGVVSGPEAAQGSAGIDLTKHFLDVGITSIRNHDYLDDRLDIEQILQ
                     VPDEKTYPSWLSADANNPKHYYWDESDEQIDKIIGGGFELLLRLGGEAWCPQRPHAFH
                     GPQDMVMEKNWMVAADNVLKRYKKWKGKTALSYIAIWSEFPGVAYWDRTAQEFVAFWT
                     KAINVFKYNHPDLKFGGPGFSAEATDEVRRGISGVVAEFLKQLYLRKTKPDWIGWHLY
                     ANDPSEALEAARAYELLLAGKAPFQNVPWAGKFFFDEVTLLVDSYGVRTANEE"
BASE COUNT          311 a          348 c          241 g          324 t
ORIGIN      
        1 tttcctcgtt cgctgtgcgg acaccgtagg aatcgaccaa cagcgtcacc tcatcaaaaa
       61 aaaattttcc ggcccacggg acattctgga agggcgcctt tccggccaat aagagctcat
      121 aggctctggc ggcctccagc gcctcagagg ggtcgttcgc atacaaatgc cagccaatcc
      181 agtcgggttt ggtctttctg aggtacaact gcttcagaaa ttctgccacc accccgctga
      241 tgcccctgcg cacttcatct gtggcctccg cgctaaaacc aggtccgcca aacttaaggt
      301 ccgggtgatt gtatttaaaa acattgatgg cctttgtcca aaaggcaaca aattcttgcg
      361 ctgttctatc ccaatacgca accccgggaa actcagacca gatggcaatg tacgacaggg
      421 ccgtcttgcc tttccatttt ttgtatctct tcaacacatt gtctgccgct accatccagt
      481 ttttctccat caccatgtcc tggggcccat gaaaggcgtg tgggcgctga gggcaccaag
      541 cctcaccgcc cagacgtagc agaagttcaa aaccaccgcc gataatttta tcaatctgtt
      601 catcgctttc gtcccaatag taatgcttgg gattgttcgc atcggcactc aaccaggaag
      661 gatacgtctt ttcatctggt acctgcaaaa tctgttcgat gtccagacgg tcatcaagat
      721 aatcatgatt acgaatactc gtgattccca catccaagaa atgttttgtg agatcaattc
      781 ccgcagaacc ttgcgccgcc tccggcccag acaccacgcc caacaggggc ttaatgaggg
      841 cgttcccgtc cctcagagac aaatccaaca ccaggtcatt cgcatccatt atcgagcccg
      901 cagcaacggg cgtagccgtc tgagccgctg cctcggctgt caaaaagaca caaaagaaac
      961 agctcaataa aaaaattatt ttattcatgc cacaacacta accttatgaa atacaaaaaa
     1021 caatcattgc cttttttcct tcacaaaaaa tggcaggatt attttcgttg caacatactc
     1081 aaaatatctt taaggaatgt ccttcatgaa acatcctttt ttaaattgtc tcttgttttt
     1141 tttcctgtca ttgtttttgt tccctttttc tcctgctcag gctgccgatt gttctatgcc
     1201 tcccctaggc tttacacaaa gcgc
//
LOCUS       DATDUN010000656         1058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_567354, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000656 DATDUN010000000
VERSION     DATDUN010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567354"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VJC18_09180"
     CDS             complement(<1..508)
                     /locus_tag="VJC18_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45596.1"
                     /translation="MPNILIRVGNVEFRSNRADICLTTSACNKKKKLSTVASVLAFGF
                     LPFDYSGLAFRGTPLLAGGLPQWCSFLGSSDTPRDTPYLTGFNREGRIVDGAGNVRLA
                     QPGLHSPSTASTKNFSQHNFPNTQNDSGSVANARRRHTAQAKTETDTTTATTAAGDAT
                     DDAENNNKR"
BASE COUNT          253 a          278 c          284 g          243 t
ORIGIN      
        1 cgcgtttgtt gttgttttct gcgtcgtccg tcgcgtctcc tgctgctgtt gttgctgttg
       61 ttgtgtcggt ttctgttttg gcttgcgccg tgtgcctgcg ccttgcgttc gccacgctcc
      121 cgctgtcatt ttgtgtgttt ggaaagttgt gttggctaaa attttttgtt gaagcggttg
      181 aaggcgaatg caagcccggt tgcgccaagc gcacgttacc tgcaccatcc actattcttc
      241 cctcacggtt gaaacctgtg aggtagggcg tgtcccgcgg ggtgtcactg gatccaagga
      301 atgaacacca ctggggtaaa cctccggcaa gaaggggcgt cccccgaaaa gccagtccgc
      361 tgtaatcaaa gggcaggaaa ccgaaggcca acactgaagc cacggtcgac aatttttttt
      421 ttttgttgca cgcagaggtt gtaaggcaaa tgtcggcccg attcgatcta aattcgacgt
      481 ttccgacacg aatcaaaatg ttgggcacaa gttgcagtcg ttgtgacatt ttgtctgtcc
      541 cgcaaggggt aaaccccgca ctttgaaagt gcgtcctctg gaaggcgatg cgacattagg
      601 ccagtgacga ccatcgcaac catatttttc gacccgtcct cggtttcagc aagccgccac
      661 agtgacataa aagccaaagc agccaaggat accgttgcca tgaatccaag cccacaggaa
      721 gatcccgttc cttggggacg cggtaccgga agatgaattt ttcgggtcgt cgcacatcag
      781 cgtttccaca acgacataat cgaagaaaca tgcaaattct gcaggaccaa tgcacgatgt
      841 ttgtggggag aaaagtggaa ctcgtctgcg aacggacgtg gttcagctcc aaacggcagg
      901 agcgaaatcg gcgacaaaaa acccacagcg caaccgccaa caccaacgac gagacatcat
      961 cagcattagc gccgctatat cacaatgaaa aaaaaattgt gatatgacgg gggtcttcgg
     1021 caagggccgc agccgaccct cgagcggccc cacagcgg
//
LOCUS       DATDUN010000657         2307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_366674, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000657 DATDUN010000000
VERSION     DATDUN010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366674"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VJC18_09185"
     CDS             complement(<1..701)
                     /locus_tag="VJC18_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45597.1"
                     /translation="MKSWVDIKVLQCVIMSMAVAVLSVSCGGGVDLDGNNNSDNDGDE
                     ITTAAFPNNLAVASPFSLTSTADPSVSATVKYATANAFTPSYKAITDQIGDILSGSAG
                     DCSFDPYDFFQQEENTACFGPSLAYEGHPEAEEGEEDSGVLPIGDLGIWLENEVGSDN
                     ACSASELDAKLAGTQALSTATLMAVASMICTANANDIALPDATNPSSVDMLDAMNAME
                     IANVTFDTASVSYSAA"
     gene            947..1411
                     /locus_tag="VJC18_09190"
     CDS             947..1411
                     /locus_tag="VJC18_09190"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HLD45598.1"
                     /translation="MQNHELFMRLALIEAQKAYADNEIPIGVVVVKDGQVIASDYNTC
                     EADHSALSHAELKALRAASKLQNNWRLSDCDLYVTLEPCPMCLGALFQTRIRTLIIGC
                     FDHKRQNEKLSAFPSLKDKDSLTDNNHTIQILGGILEKECAEVLRKFFKNIR"
     gene            complement(1483..1941)
                     /locus_tag="VJC18_09195"
     CDS             complement(1483..1941)
                     /locus_tag="VJC18_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45599.1"
                     /translation="MSSITGSGGIIRLDTSRMNEYLYADKPKLNFMQKLGRGFGKALS
                     FLGPIGAAVTAIAVPGIGLPIAAGIYGLSSAAGTLTQRAQAKDAYEMQTYQQQVSQLP
                     VMTPGLFEQAPATGAQTDFIVPTELKEQTELTILNRESAQNGSVEGFNFY"
     gene            complement(2086..>2307)
                     /locus_tag="VJC18_09200"
     CDS             complement(2086..>2307)
                     /locus_tag="VJC18_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45600.1"
                     /translation="DPNAEYVSFGGMMLDAASAKKIKLIAAVLLVVLLLLAAGFIYVL
                     LRMSRMRRQSSVPAVIEQSDSDDQKLLGS"
BASE COUNT          617 a          602 c          519 g          569 t
ORIGIN      
        1 gccgcggaat atgacacgct ggccgtgtca aaggtcacgt tcgcaatctc catagcattc
       61 atggcgtcga gcatgtccac actcgacggg ttcgtcgcgt caggcaaggc gatgtcgtta
      121 gcgttggccg tgcagatcat cgaggcaaca gccatcaacg tggccgtcga caaggcttgc
      181 gtccccgcga gtttggcatc gagttccgac gcggaacagg cattgtctga acccacctca
      241 ttttcgagcc agatacccaa gtccccaatc ggcaaaaccc cactatcttc ctctccttct
      301 tcggcctctg ggtggccttc atatgccaaa gaggggccaa agcacgcggt attttcctcc
      361 tgttgaaaaa aatcataggg atcaaacgaa caatccccgg ccgaaccact cagaatgtcg
      421 ccgatctgat ccgtgatggc cttgtaggaa ggcgtgaacg cgttcgcggt cgcgtatttg
      481 accgtcgccg acacactggg atcagcggta gacgtcaatg aaaacggaga cgccaccgcg
      541 agattgttgg gaaaggcggc cgtggtgatt tcgtcgccat cattatcaga attgttgttg
      601 ccatccagat ccacaccccc gccgcaggaa actgagagca cagcaacagc catggacatg
      661 atgacacatt ggagaacttt gatatctacc catgatttca taaaaccccc tttaaataat
      721 gttcaaccca ttgctgttgc aaatctcttg ccaaatcgaa cttaaaaaaa cactttgtaa
      781 tgattgttga aaattgcact caacgattaa cgtggaaaaa aaagcattca tgaactgaca
      841 ctatcgcagt ttgattctgc aaaaatatat ttctgatgga attgtgattt ttttctaaat
      901 tcaaaaatga ctttactcat ttttcttaac caactaacct cccctcatgc aaaaccatga
      961 actgtttatg cgattagccc tgatcgaagc tcaaaaggca tatgcagaca atgaaattcc
     1021 catcggtgtt gtcgttgtga aagacggcca ggtcatcgcg tcggattata atacctgtga
     1081 ggctgatcat tctgccttaa gccacgccga actcaaggca ctgagagccg cgtcaaagct
     1141 acaaaataat tggcgtttga gtgattgtga tttgtacgtc acgctggaac cctgccccat
     1201 gtgtctgggt gccttgttcc agacacgaat caggaccctt attatcggtt gtttcgacca
     1261 caagcggcaa aacgagaaac tatcggcatt tcccagcctc aaagacaaag actctctcac
     1321 cgataacaat cacaccattc aaatccttgg gggcattttg gaaaaagaat gtgcggaggt
     1381 attaagaaaa ttttttaaga atatacgata gcgcacaaaa cctgtctttt attgcgagaa
     1441 gtcctgggcg aagccaaagg acgacgaagc aatccacgtt catcaataga aattgaatcc
     1501 ctctacggac ccattctgcg cgctctcgcg gttgaggatg gtgagttcgg tctgttcttt
     1561 gagttcggtg ggaacgatga aatcggtttg cgcgccggtc gccggggctt gttcaaacaa
     1621 gccaggcgtc atgacaggaa gttgggagac ctgctgctgg taggtttgca tttcgtaagc
     1681 atcctttgcc tgggccctct gcgtgagcgt gccagcggca ctcgataacc cataaatccc
     1741 tgccgcaatg ggaagaccta ttcccggaac agcaatcgcg gtgacagcgg ctccgatagg
     1801 ccccaaaaat gacaaggcct tgccaaagcc cctacccagc ttttgcataa agttcagctt
     1861 gggcttgtcg gcatacaaat actcattcat tcgcgatgta tcgagtctga tgatcccgcc
     1921 tgatcctgtg attgatgaca tatgcttaac aacccatctc cctgtccaga agctaactcg
     1981 ttgaattcac gtgcttcccg acaggaattc tgtgtctgac cttattatcg gcggtcaaaa
     2041 caccagtgtt tcgaaaaaag ctgtttttct cagtctcttg ctacatcatg aacccaataa
     2101 cttttgatca tcactatcgg attgctcaat caccgcaggg actgaacttt gtcttctcat
     2161 gcgcgacatc ctgaggagaa cgtaaataaa ccctgccgcc aacaaaagca aaacgaccaa
     2221 aagcactgcg gcaatcaact tgattttttt tgccgaggcg gcatccagca tcatccctcc
     2281 aaaactcaca tattccgcat ttggatc
//
LOCUS       DATDUN010000658         1029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_154810, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000658 DATDUN010000000
VERSION     DATDUN010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154810"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VJC18_09205"
     CDS             <1..375
                     /locus_tag="VJC18_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45601.1"
                     /translation="IHSVDTTTYALSTPLVAETDDYVDGFLTNGHGISSSINGLVLVR
                     DGSDQLHAYLSELDGNVQHVTVSDDLTAYEAETLSTSAANLTDSAALSSGDGEDSLAF
                     IVSESGALLVVDVETSDIEVMD"
     gene            558..>1029
                     /locus_tag="VJC18_09210"
     CDS             558..>1029
                     /locus_tag="VJC18_09210"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLD45602.1"
                     /translation="MNKYFEQIKRELLTQQHTWLLTGVAGFIGSHLLEFLLKHKQRVV
                     GVDNYSSGFEDNLTQVKDAVGPEAWELFSFVEGDVGDAERISKTCQGVDYVLHQAGLV
                     SPIHSVKAPLLTYESNLMATVRLFHACHTLKVKRVVYASCSSVYGDTQQKSKREH"
BASE COUNT          240 a          234 c          287 g          268 t
ORIGIN      
        1 attcattctg tcgataccac cacttacgct ttgtcgacac cgctggtggc tgagacagac
       61 gattacgttg atggtttttt gaccaacggg catggcatca gttcttcgat caatggcctg
      121 gtgctggtca gggacggttc cgatcaattg cacgcttatc tctctgagct cgatggcaat
      181 gtgcaacatg tgaccgtgag tgacgatctg acggcctatg aggcagaaac tctgtccacc
      241 tcggcggcga acctgaccga cagcgccgcc ttgtcgtccg gtgatggcga ggattcgctg
      301 gccttcatcg tttccgaatc gggagctctt ttggtggtgg atgtggagac atctgatatt
      361 gaggtgatgg attagatcat ttttcccgct cttctgtgaa gtattttttt cgggtgatac
      421 ggggagctat tatttttgac tgagtcacgc atagcgtgac gtagcgagct tgtgagcgaa
      481 aagtcaaaaa aatagcgacc tggagcagga cctgaaaaaa ataaaggcag ataaagattt
      541 ttggcgggtg ctgaaatatg aacaaatatt ttgagcaaat caaacgagaa cttctgacac
      601 aacagcatac ctggctcttg accggtgtgg cggggttcat cgggtcacat ctgttggagt
      661 ttttgctcaa gcacaagcaa cgtgtagtgg gggtcgataa ttattccagc gggtttgagg
      721 acaatctcac acaggtcaaa gacgctgtcg gcccggaggc ctgggagttg ttttccttcg
      781 ttgagggcga tgtcggagac gctgaacgca tcagcaaaac ctgtcagggg gttgattacg
      841 tgttgcatca ggcggggttg gtctcgccca tccattcagt caaagccccc ttgttgacct
      901 atgaatccaa tctgatggcc acagtcaggc tgtttcacgc ctgtcacaca ctcaaggtca
      961 agcgtgtggt gtacgcctcc tgttcctctg tttacgggga tacccagcaa aaatcgaaga
     1021 gagagcata
//
LOCUS       DATDUN010000659         1468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_198462, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000659 DATDUN010000000
VERSION     DATDUN010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198462"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VJC18_09215"
     CDS             complement(<1..237)
                     /locus_tag="VJC18_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45603.1"
                     /translation="MTFSLNTLLPSYALFTLIGCDSKDKPDDSSVVGDTAESDVPVDT
                     QDTGEPTDTDTDDDTGIPFDPAPSVGCEDSTFTTI"
     gene            complement(240..1283)
                     /locus_tag="VJC18_09220"
     CDS             complement(240..1283)
                     /locus_tag="VJC18_09220"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLD45604.1"
                     /translation="MARADTTVNDSFPKIPDYKILRQIGQGSFSKVYLAESVATGEKY
                     AIKVLSPGLVADPRVLTKFYKEAVAYQTIASEHVVKLHSFVSRTGEPPYMILEYVPGG
                     TMLQWSAEHRDTKKYHKQFAEVMLACAKGIIDINQAGLLHRDIKPDNIFMTTEGTPKI
                     GDLGLSKVQAVQTSSVGVGTLVYQSPEQLTSSDIDVRSDLYSLGATMYYAFTGQAPYY
                     GTPATQGDSIKERALWLLSAPQLDPWVVNKKIPEPLRRIILLLTMRDRNDRYQTPEEL
                     VEDLGLYLKGKTLKHATRKAVAMSSSQLTLVPPQKEGDVVVPRGSFMSELGLLEDVGR
                     LARFPNTRKQRGA"
BASE COUNT          347 a          381 c          366 g          374 t
ORIGIN      
        1 aatggtggtg aatgtggaat cctcacagcc cacactgggt gcaggatcaa aaggaatacc
       61 cgtatcgtcg tcagtgtctg tgtcagtggg ttccccagtg tcctgcgtgt cgacgggaac
      121 atccgattcg gctgtatcac caacgacaga cgaatcatcg ggtttgtcct ttgaatcaca
      181 acctatcagc gtaaacaagg catatgaggg caaaagtgtg tttaatgaga atgtcatgtt
      241 catgctcctc gttgttttct ggtgtttggg aaacgggcca aacgaccaac gtcttcaagc
      301 aaccccaact cactcataaa agacccacga ggcaccacaa catcaccttc tttttgcggg
      361 ggaaccagtg tgagttgcga ggaactcatc gcgaccgcct tgcgtgtagc gtgcttgagg
      421 gttttgcctt tcaaatacaa ccccagatcc tcaaccaatt cctctggtgt ttgataccga
      481 tcatttcgat cgcgcatggt cagcagtaaa atgatgcgtc tgaggggttc gggaattttt
      541 ttgttcacaa cccacggatc gagttgtggc gcactcagga gccacagagc cctttcctta
      601 atgctgtctc cctgtgtggc gggtgtgcca taatacgggg cttgacccgt gaacgcgtag
      661 tacatggttg cgcccagtga atacagatca ctccgcacgt cgatatcgct ggatgtcagt
      721 tgctcaggtg attgatacac caaggtcccc acgcccaccg aactggtctg cactgcctga
      781 acctttgata ggcccagatc acctattttg ggagtccctt cggtcgtcat aaaaatattg
      841 tcaggcttga tgtcgcgatg caaaagacct gcctgattga tatcaatgat gcccttggca
      901 caagccagca tgacctcggc aaattgctta tgatacttct tcgtgtcacg gtgctcagca
      961 gaccactgca acatggtgcc tcccgggaca tattccagaa tcatgtaggg aggctcacca
     1021 gtccgactga caaagctatg gagtttgacc acatgttccg atgcaatggt ctgataggcc
     1081 acagcctctt tgtaaaattt tgtcaaaacc ctgggatctg caaccaatcc cggtgacaag
     1141 accttgatcg catatttttc acctgtcgcc acgctctcag caagatagac cttggaaaaa
     1201 gaaccttggc cgatctgcct caatattttg taatcaggaa tttttggaaa cgaatcgttt
     1261 acagtggtat ccgcgcgtgc cacagctttc acctcagctc tcgcaaccac cggtgccgga
     1321 cttgctggct tgactgctgg agctaacgga attacctgcc ttggtgccga aacaggctct
     1381 gatacgggag ccagtggcaa agcgtgtctg ggtggagtag atcctactgg aatcactgct
     1441 ggtttaggag ttgcctgtcc tagtgctg
//
LOCUS       DATDUN010000660         1285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_366742, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000660 DATDUN010000000
VERSION     DATDUN010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366742"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..>1285
                     /locus_tag="VJC18_09225"
     CDS             87..>1285
                     /locus_tag="VJC18_09225"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HLD45605.1"
                     /translation="MDLKEHTRAYARVQKAIQNYQQGQMVIMVDDEDRENEGDVTVAA
                     EKVTHEHVNFMAKHARGLICLSLTEEQVEKLELPLMVNRNLSPYQTGFTVSIEASSGV
                     TTGISAADRAHTIQTAIKPSAKPNSVVSPGHIFPIRARSGGVLVRAGHTEGSVDLARL
                     SGLNPSGVMCEIMNDDGTMARLPELEEFSRKFDLPLVTIEDIIKYRTRYESLVHLVAQ
                     SKLPTDFGQFSIKVFENDVDDYHHIALLMGEIDGTKPVLVRVHSECMTGDVFGSARCD
                     CGSQLQASMRQIAAEGCGIVLYVRQEGRGIGLVNKIRAYHLQDQGRDTVEANEDLGFK
                     PDLRDYGIGAQVLAHLGVKDIRLLTNNPKKIIGLEGYGLHIIDRVAIEIEPGEHNQHY
                     LEAKKKK"
BASE COUNT          345 a          311 c          338 g          291 t
ORIGIN      
        1 gcgtcgtgcc aaggcttcat gaattgaccc tgccccactc aataaacacc cttttttttt
       61 ccactcaaat aggctagggc ggcgccatgg atttaaaaga acacacgagg gcttacgccc
      121 gggtccaaaa ggccattcaa aactatcaac agggtcaaat ggtcatcatg gtggatgatg
      181 aagaccgcga aaatgaaggt gatgtcaccg tcgccgcaga aaaagtgacg catgaacacg
      241 tcaacttcat ggccaaacac gcccgaggcc tcatctgcct gtctttgacg gaggagcagg
      301 tagaaaaact cgaattgccc ctcatggtta atcgcaactt gagtccctat caaacggggt
      361 tcacggtatc cattgaagcg agcagtggcg tcaccacggg aatttccgcg gccgataggg
      421 cgcataccat tcaaacagcc attaagccct ctgccaaacc caactccgtt gtgagtccgg
      481 gtcacatctt tcccatcagg gcacggagcg gcggggtgtt ggtcagggca ggacacaccg
      541 agggatctgt agacctggcg cgcctttccg gtcttaaccc cagcggcgtg atgtgtgaga
      601 tcatgaacga tgatggcacc atggcccgcc tgcctgaact ggaggagttt tcgcggaaat
      661 tcgatctgcc tttggtgacg atagaagata tcatcaaata ccgcactcgc tatgaatcgc
      721 tcgtgcacct ggtggcccag tcaaaactac caactgactt tggtcagttt tcgatcaagg
      781 tatttgagaa cgatgtcgac gattatcatc acatagccct tttgatgggc gagattgatg
      841 gcacaaaacc agtcttggtc agggtacact cagagtgtat gacaggtgac gtttttggtt
      901 ctgcgcgctg tgactgtggt tcacagctac aggcctccat gaggcaaatt gccgcagaag
      961 gttgcggaat tgttttgtac gttcgacaag agggaagggg aattgggttg gtcaataaaa
     1021 tcagggccta ccacttgcaa gatcagggac gggataccgt cgaggccaat gaggatttgg
     1081 gtttcaaacc agatttacgc gattatggca ttggtgccca agtgttggct catctgggcg
     1141 taaaagacat tcgtttgctg acaaacaatc caaaaaaaat tattggtttg gaaggttacg
     1201 gattgcacat cattgaccga gtcgccatag agattgaacc aggcgaacac aatcagcact
     1261 acctcgaagc caaaaagaaa aagat
//
LOCUS       DATDUN010000661         1614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_366817, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000661 DATDUN010000000
VERSION     DATDUN010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366817"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1299
                     /locus_tag="VJC18_09230"
     CDS             145..1299
                     /locus_tag="VJC18_09230"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45606.1"
                     /translation="MLTSLPIKEQIETRLKADFPLIQAILGPRQVGKTTLAEQIFDRW
                     AGPKIFASADLPSLPPLEWLRFQTEQAMQKGPGTLLVVDEIQKITNWSSQIKSFFDSV
                     RNKIPIKILLLGSSSLFLQKGLNESLVGRFEVIPVLHWSYPLCQNAFGWDLNTYLKFG
                     GYPGAVAFIEDEDRWRNYILHSIIEPVLGRDILGQVSIGKPALFRQSFELAINYPSQI
                     VSYTKMLGQLQDKGNATTIQHYLNLFEASFLIRLLKKYTGSTLQTIGSPPKIIVLNPA
                     LTHAYNTVSKIDNDPRWYGLIFESVVGSALAQTPGAELYYWREGSAEVDFVLKTPKGV
                     WGIEIKSGLKSNFTKGVNEFHKKFPTAHVEQWNFDTACKFLSGNHSPWNH"
BASE COUNT          417 a          449 c          321 g          427 t
ORIGIN      
        1 tgcccccccc gcgtttctgc gacccttgag cttctgcgat ccttcccccc ctcccgttcc
       61 ccttttgcca ttttattaga tatatctaac aaattcatta gatcaatcta ttgacattga
      121 ttaacgttat aattatactt agttatgcta acgtcactcc ccataaaaga gcaaattgag
      181 actcgtctta aggcagattt tccgctcatt caggcaatct tgggtcctag gcaagtaggc
      241 aaaacaacgc ttgccgagca aatatttgac aggtgggctg gtccaaaaat ttttgcctcg
      301 gcagacctgc cctctctacc tcctttggaa tggctccgtt ttcagacaga acaggccatg
      361 caaaaagggc ccggcacatt gttggtggtt gatgaaattc aaaaaatcac gaactggagt
      421 tcgcaaataa aatctttttt tgattccgtg cgtaacaaaa ttcccatcaa gatattactg
      481 cttggttcat ccagtctttt cctgcaaaaa ggtcttaacg aaagtctggt ggggaggttc
      541 gaagtcattc ccgtcctgca ctggtcgtac ccgctttgtc aaaatgcctt tggatgggac
      601 ttgaacacct acctcaaatt tggtgggtat ccgggagctg ttgccttcat cgaagacgaa
      661 gatcgatggc gcaattatat tctccacagc attatcgaac ccgttttggg ccgagacata
      721 ttgggtcagg tttccatagg aaaacccgcc ttgttcagac aatcttttga actggccatc
      781 aattatccgt cacagattgt gagctacacc aagatgttgg ggcaattaca agataagggc
      841 aacgcgacca ccatccagca ctatttgaat ctttttgaag catcattttt gatccggctc
      901 ttaaaaaaat acacgggttc cacactgcaa acaatcggat caccacccaa aatcattgtg
      961 ttgaatcctg ccctcacgca tgcctacaac accgtctcca aaatcgacaa cgatccccgc
     1021 tggtatggac tcatttttga atcggtcgtg ggcagtgcct tggcgcagac cccgggagcc
     1081 gaactttatt attggcggga aggttccgcc gaagtggatt ttgtgctcaa aacaccaaag
     1141 ggcgtttggg gtatcgaaat aaaatcagga ctcaaatcaa atttcaccaa aggggttaat
     1201 gagtttcaca aaaaattccc caccgcacac gtcgaacaat ggaacttcga caccgcctgc
     1261 aaatttctca gtggcaatca ctcgccgtgg aatcattgat gcaaagtggc acccaccttg
     1321 attcgggccg gtcgagcccg gcccctacgt tgcacagcca ggccattgcc acaattgccg
     1381 gttgcaacaa ttgcgcaatt atccccctcc ccccattgac cctttgaccc tttgactttt
     1441 tgaccctttg accctttgcc ccccccgcgc tccccactag ccgctggcac tggtcactgt
     1501 tttacgcacc gcgtcatacc cgttaatccc ttgtcacgtg caacctttaa caactcttaa
     1561 caacgcttac cactcctccc caaaacccta tcagattcat gacaacagca aatt
//
LOCUS       DATDUN010000662         1535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_491570, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000662 DATDUN010000000
VERSION     DATDUN010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491570"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VJC18_09235"
     CDS             <1..1111
                     /locus_tag="VJC18_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HLD45607.1"
                     /translation="VAGNFSAFYIGMLIAGLMAGAYFTSLFIPAAVIHYPSIFAFGLV
                     AFGFLCMGPVNIAVDSYGPVTDNAQSIFELAQTEKIPGIKENIKKRFGFIPDFVRGKH
                     YLEANDSAGNTFKATAKPVLIGTAVVGATTMIFSIILLLESKGLLHVQLTDAPILLGL
                     ICGGAVIYWFCGASMQAVTTGAYSAVEFIKKNMNLKKATADIEDSKTVVKICTVYAQK
                     GMWNIFIALMAITLAFAFLNPNFFVAYLISIACFGLYQAIYMANAGGAWDNAKKVVEV
                     DLKDKGSDLHVATVIGDTVGDPFKDTSSVSLNPIIKFSTLFGLLATEIAIEMAVHARE
                     TGSVNYAPYLSIPMFIIAMVFVWRSFYKMRIPEKL"
     gene            1150..>1535
                     /locus_tag="VJC18_09240"
     CDS             1150..>1535
                     /locus_tag="VJC18_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HLD45608.1"
                     /translation="MSHIITLTSDFGNADHYVGAMKGAILRINPNVTLVDISHEIPSF
                     GVPIAAYVIGSSFLAFPRGTIHVVVTDPGVGTSRRPILLCTENYFFLAPDNGVLSLAA
                     ERDGARAIYHLDKPEYYLDHLSATFHG"
BASE COUNT          333 a          366 c          409 g          427 t
ORIGIN      
        1 tgtggcgggc aatttcagtg ccttttacat tggcatgctg atcgcgggac tcatggcggg
       61 agcgtatttt accagcctgt tcattcctgc cgctgtgatc cattacccgt ccatctttgc
      121 gtttggtctg gtggcctttg gtttcttgtg tatgggcccg gtcaatatcg ccgtggattc
      181 ttacgggccg gtcactgaca atgcccagtc catttttgag ctggcgcaaa ccgagaaaat
      241 ccccgggatc aaagaaaaca tcaaaaaaag atttggtttt atacctgatt ttgtcagggg
      301 taaacactac ctcgaggcca acgattcggc gggcaacacg tttaaggcaa cagccaaacc
      361 tgtgctcatt ggcaccgccg tggtgggggc cacgaccatg atcttctcga tcatattgct
      421 tttggagagc aaaggcctgt tgcatgtgca actgaccgat gccccgatcc tgttgggctt
      481 gatctgtggt ggggctgtca tttactggtt ttgcggggca tccatgcagg cggtgaccac
      541 gggggcttac agcgcggtcg agttcatcaa aaagaacatg aacctgaaga aggccactgc
      601 tgacatagaa gattccaaga ccgtcgttaa aatttgcacg gtgtatgctc agaaagggat
      661 gtggaatatc ttcattgcac ttatggccat tacgttggcg tttgcgtttc tcaaccccaa
      721 tttttttgtg gcgtacctga tctccatcgc ctgctttggt ttgtatcagg ccatttacat
      781 ggctaacgcc ggaggggcct gggataatgc caaaaaggtg gtcgaggttg atctcaaaga
      841 caaagggtca gacttgcatg tggccacagt gattggtgat accgtgggag atccttttaa
      901 ggatacctca tcggtttcgc tcaatcctat tatcaagttc tcaacactct ttggtctatt
      961 ggccacagag atagcgattg aaatggctgt tcacgccaga gaaacgggta gtgttaatta
     1021 cgcgccctat ttgtctattc ccatgtttat catcgcgatg gtttttgtgt ggcggtcttt
     1081 ttacaaaatg cggatacccg aaaaactgtg acgggtgccg tgtctcggta aagggcggtc
     1141 ttaaagccta tgtcccatat catcaccctc acaagcgatt ttggaaatgc cgatcattat
     1201 gttggcgcca tgaaaggggc catcttgagg atcaacccca atgtcacgtt ggtggatata
     1261 tcccacgaga ttcccagctt tggagtgccg atagcggcct acgttatcgg ttcctctttt
     1321 cttgcctttc ccagggggac gattcatgtg gtggtgacgg accccggtgt gggcacgtcg
     1381 cgtcggccta ttttattgtg taccgaaaac tattttttcc tggcgccaga taatggggtg
     1441 ttgagtctcg ccgcggagcg cgatggcgcg cgggcgattt atcacctcga caaacccgaa
     1501 tattacctcg atcatctgag tgcgaccttt catgg
//
LOCUS       DATDUN010000663         1413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_75304, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000663 DATDUN010000000
VERSION     DATDUN010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75304"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VJC18_09245"
     CDS             complement(<1..581)
                     /locus_tag="VJC18_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HLD45609.1"
                     /translation="MKTGIFWDERCTKHEMSRGHPESPQRLWAIKQVIDANPRLVHIE
                     PRLASFEEVSSIHTEKLVRLVDESRGKSNTCFDPDTFSNKHTATAAFLSAGSTIALAE
                     KVEKGEWDNGFSFARPPGHHAEHDCVMGFCFFNNIAIAAQQLIEKHHKKRIAIVDYDV
                     HHGNGTQASFYDRDDVLFCSVHHYPFYPGTGGS"
     gene            919..>1413
                     /locus_tag="VJC18_09250"
     CDS             919..>1413
                     /locus_tag="VJC18_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HLD45610.1"
                     /translation="MEYDQDDSSGGGFGNKYSKKSIGDPSKIKQVPALPLRDIIIFPH
                     MVVPLFVGREKSINALDQAMNEEKDILLITQINAKTNDPKPEDIYSVGCLGSIVQLLR
                     LPDGTVKVLVEGNKRARVHRYITTEPVFIAEVEEIEEDKEMTVELEALMRSVKTTFET
                     YVKLN"
BASE COUNT          420 a          326 c          317 g          350 t
ORIGIN      
        1 aacgagccgc cagtccctgg ataaaacggg taatgatgca cggaacaaaa caggacgtca
       61 tcacggtcat aaaaagaggc ctgtgtcccg ttgccgtggt gcacatcgta atccacaatc
      121 gcaatgcgtt ttttgtgatg cttctcgatc aactgttggg cggcaatggc aatgttgtta
      181 aaaaaacaaa agcccatgac acaatcatgc tccgcgtgat gtccgggtgg acgcgcaaaa
      241 gaaaaaccat tgtcccattc ccccttttcg accttttccg ccagcgcgat tgtactgcca
      301 gccgacaaaa atgcggcagt ggccgtgtgc ttgttggaga atgtgtcggg atcaaaacac
      361 gtatttgatt ttccacggct ctcatcgaca agccgtacca gcttttcggt atgaatggag
      421 ctgacttctt caaaagaggc cagtcgtggc tcgatgtgaa ccaaacgggg atttgcgtca
      481 atgacctgtt taatggccca aagtctttgt ggggactcgg gatgtcctcg tgacatttcg
      541 tgttttgtac acctttcgtc ccaaaaaatg cctgttttca tggggtcaag ctaccatatt
      601 cttatttctt tgtcatcaca ccctacgcag ggatgacaaa attccagaaa atcatccaaa
      661 tcggcagcat caataaacag ctccagtttt tttccctctt tactgcaaaa atagatagcc
      721 aataacaaat aaatgatcga taacctttca ataaatcgtg aaaacacaat taaacgtaat
      781 atcaacttgc caatagggtg atttgatctt atcattgatg agactcaaaa aaaacagcca
      841 catctggcaa aaaatttgtg tttgtggcaa aaaaaatcaa gaattggtca gtaaatttta
      901 agaagagagg atgtttctat ggaatacgac caagatgaca gctcaggcgg tggatttggc
      961 aacaaatatt caaagaaatc cattggtgat ccctcaaaaa tcaagcaagt accggccctt
     1021 ccgctcaggg acatcatcat ttttccgcac atggtggtcc ccctctttgt ggggcgggaa
     1081 aagtccatca atgccctcga tcaggccatg aacgaggaaa aggacatatt gctcatcacc
     1141 cagatcaatg ccaaaaccaa cgatcccaag ccggaggata tttacagtgt aggttgtttg
     1201 ggcagtatcg tacaactgct caggcttcct gatggcacgg tcaaggtcct ggtggagggc
     1261 aataaacggg cgcgtgtgca cagatacatc actacggaac ctgtttttat tgcagaggtt
     1321 gaagagattg aagaagacaa ggaaatgacg gtggaactcg aagccctcat gcgttcggtc
     1381 aaaaccacct tcgaaaccta tgtcaaactc aac
//
LOCUS       DATDUN010000664         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_319864, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000664 DATDUN010000000
VERSION     DATDUN010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319864"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="VJC18_09255"
     CDS             complement(<1..1746)
                     /locus_tag="VJC18_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45611.1"
                     /translation="MKTSHIINRWRVLVFVITVLALLGSFSNPSLADTGVQTTTAPSL
                     TSSPSRVDAHFSKNAWIYTKIDDTGYAQLYLQHLDSSAVLTPTTPLNEYRLTTSAFNK
                     SNPKWGPDISSMYIYTTYPKGTKTQCEGDMSAYYLGDSDSDGSSEIHFICIDKFPLPA
                     VQVIADVKLTGWDTVTNYNVTDYDVPHYANTAKNLYTNPINTYGVVFTADDRKLHILM
                     HEVNQIAQDISNLTASLIVYDYSDSSPANGPYVWASRSEPFSFYSQPRFDAYGYNIVF
                     VGQMKGEDTNEIGTISVGGFNERQLTDLNLVLSNPQFIESRLATATSNPYIYGISPEI
                     LFEVTDNSITRLARFKTTTYIAGTTNWCNVADYITDDKYSRQNLVNLVDPRGGYTYNK
                     YALEFAYTLSHADGLRDIYASGLSFLQSCAGVSEAGTNRTLGTTAITEITTYIDGTST
                     LITDETQLTCLHDNTNPVILPSVYYDDSEPPVYATHSTVTGRTDIVMNRTLSDTSEKV
                     LVHLFNVDKSTGEECDSCYEDADGSPIEDINGFEDGDGNKDSCEEVPQPSETPACNPA
                     SLLADPTGDFDGDGIT"
BASE COUNT          514 a          431 c          577 g          616 t
ORIGIN      
        1 ggtgatgccg tcaccgtcga aatcaccggt ggggtcggcc aagagagatg cgggattaca
       61 ggcaggggtt tcgctgggtt gaggaacttc ctcgcaggag tccttgttcc catcgccgtc
      121 ctcaaagccg ttaatatcct caatgggaga gccatctgcg tcctcatagc aggaatcgca
      181 ttcttcaccg gtgctcttat ccacgttgaa gaggtgcacg aggacttttt cggaggtgtc
      241 cgacagggtt cgattcatca caatgtctgt gcgtcctgtg actgtcgagt gggtcgcgta
      301 aacgggaggt tcggaatcat cataatatac ggaaggcagg atgacagggt tggtgttgtc
      361 atgcaggcag gtcagttgtg tttcgtctgt gatgagcgtg ctggtcccat caatgtaagt
      421 cgtgatttcc gtaatggctg tagtgcccaa ggtgcggttt gtccctgcct cgctgactcc
      481 agcgcaactc tgtaaaaaac taagaccaga agcgtagatg tctctcaagc cgtcggcatg
      541 tgagagtgtg tacgcgaatt caagtgcgta cttgttatag gtgtacccgc cacgtggatc
      601 gacaaggttg accagatttt ggcgcgagta tttatcatct gtgatataat ctgccacatt
      661 gcaccaattg gttgtgcctg caatgtaggt ggtggtttta aatctggcca atcgtgtgat
      721 gctgttatca gtaacctcaa acagaatctc gggagaaatg ccgtatatgt aaggattgct
      781 tgttgctgtc gcaagacgtg attcaatgaa ttggggattc gagagaacga ggttcaaatc
      841 tgtcaattgc ctttcattga aaccgcccac agagattgtg ccaatttcat ttgtgtcctc
      901 acccttcatc tgtccaacga acactatgtt gtagccatag gcatcaaacc ggggttgact
      961 gtaaaatgaa aaaggttcgc tcctgctggc ccaaacgtag gggccgttgg ctggtgatga
     1021 atcggaataa tcatacacga tcagacttgc ggtgaggttg ctgatgtctt gcgctatctg
     1081 gttcacttcg tgcatcagga tgtgcaattt gcggtcgtcc gcggtaaaga caacaccata
     1141 agtgttgatg ggattcgtgt acaggttttt tgctgtattg gcataatggg gaacgtcgta
     1201 gtcggtgaca ttgtaattcg tgaccgtatc ccaaccggtg agtttgacat cagcgatgac
     1261 ctggacagct ggaaggggaa atttgtcgat gcaaataaaa tgaatctctg agcttccatc
     1321 actatcagaa tccccaaggt agtacgctga catgtcccct tcacactgcg tttttgtgcc
     1381 tttggggtac gttgtgtaga tgtacatgga gctgatatca gggccccact tgggattcga
     1441 tttgttgaag gccgaagtgg taagtctgta ttcgttcagg ggggttgtgg gagtcaggac
     1501 agccgaactg tccaaatgtt ggaggtacag ttgcgcgtac cctgtatcgt cgattttggt
     1561 gtatatccag gcattttttg agaaatgggc atctaccctc gatggagagc tggtcaagga
     1621 tggtgctgtt gtggtttgaa cacctgtatc agcaagtgaa gggttcgaga acgatcccaa
     1681 tagggccagt acggtgatga caaataccaa aacacgccaa cggttgatga tatgactcgt
     1741 tttcataaaa tgctcctcta cagtcatatt atagcaatat tcgttccatt atggggtgtt
     1801 attttaccta aaactactgg catgcactgt tgtaaaaaga ctagcaatat aataaagata
     1861 cagtgttgtt atgttgtgtg acgttaggtt tatagctggt ggcgggattg tctgaggaca
     1921 aacttcctca aaatgagaag attaggtaaa taaattcctc atattgagac tgtattattt
     1981 tcttttcttt ttcagatcct tgaacaagaa aatcttcttt tcgagttggc cggcattgtt
     2041 tttgaccatc atggtcacgc cttttggttt tttggtaggg ctgaaataaa gcacaccgcc
     2101 aagggtcatg ccggggggaa gtttgttgat gttggtga
//
LOCUS       DATDUN010000665         1653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_236238, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000665 DATDUN010000000
VERSION     DATDUN010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236238"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="VJC18_09260"
     CDS             complement(<1..1386)
                     /locus_tag="VJC18_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45612.1"
                     /translation="MKTLIFILSFIVSCTLVWDSLATKPNTSQQINKTAPTKTVTTTL
                     VPKDILNPQSLTQAIITQSGNYNPATDTLLLMGTDKQKEIRIEYKNNLITYLNLGAIK
                     SPSRDVTQAPTEIPKTQLDDVLKRIGLSPVVWKHSHTIQNIATKRNADGTVIKTMLHS
                     SILSYTSEQGEKLNILVGKTTSAEYDTLAILYKTKTDAFRNALSSTTKQQTDNKTTTT
                     EPLSHFGNAIASQDIQGVPDEQICSKYFPNSSSRKYSATAKFFGIPGNSDHLNENHFK
                     NNKIIPNMEKICFTHSDYDSDGILTDDNDNAPDRPSLFDTGNAAQSASFYIHMSVAML
                     MPAGLFHPCYGQYGLVDNNADYDTPPHAGTLFGPPIGLYPPALTSCWIEEEIDNDPPA
                     YLYNMSSFSASAENNWNNLVTGPHSLKVYIGNLSHGNRFTEAMFDHFFTALGHRTCVS
                     WNFFGSLCKPNG"
BASE COUNT          397 a          296 c          442 g          518 t
ORIGIN      
        1 gccattgggc ttgcagagcg agccaaaaaa attccatgat acgcaggtcc tgtggcccaa
       61 agctgtaaaa aaatggtcga acattgcttc ggtaaatcga ttgccatgag agagattgcc
      121 aatatagact ttgagggaat ggggacctgt gaccaagttg ttccaattat tttcggcaga
      181 ggccgaaaaa ctggacatgt tgtacaggta agctggggga tcattgtcaa tttcttcctc
      241 gatccagcaa ctcgtcaggg caggcgggta aagtccaatg ggggggccaa acagagttcc
      301 cgcgtgtggt ggtgtgtcgt aatcggcgtt gttatcgaca agaccgtatt gaccgtaaca
      361 agggtgaaac aaaccagcag gcattagcat ggccacagac atgtgaatat aaaagctggc
      421 gctctgagcg gcattccctg tatcgaaaag gcttgggcgg tcaggggcat tgtcgttgtc
      481 gtccgtgaga atgccatcgc tgtcgtagtc tgagtgagtg aaacagattt tttccatgtt
      541 ggggatgatt ttattgtttt tgaaatggtt ttcgttcagg tgatcgctgt ttcccggaat
      601 tccaaaaaac ttggcagtcg ctgaatattt gcgtgatgag gaattgggaa aatattttga
      661 gcagatctgt tcatctggca caccttgaat atcttgactg gctatggcgt tgccaaaatg
      721 agaaagaggc tctgttgttg ttgttttgtt atcagtttgt tgtttcgttg tgctggacaa
      781 ggcatttctg aacgcatctg ttttggtttt gtacagaata gccagtgtgt cgtattctgc
      841 ggatgtcgtt tttccgacca ggatgttgag tttttcacct tgctcactgg tatacgagag
      901 tatgctggaa tgtaacattg tttttatgac ggttccatcg gcatttcttt ttgtggctat
      961 gttctgaatg gtatgactgt gtttccagac aacaggagaa agtccaatgc gcttgagtac
     1021 atcgtcaagt tgcgtcttgg gtatttcagt gggggcttgg gtgacatcac gcgagggact
     1081 tttgatggcg ccaagattca aataggtgat gagattattt ttatattcga tcctgatttc
     1141 tttttgttta tctgttccca ttaataaaag tgtgtctgtt gcgggattgt aattaccgga
     1201 ttgagttatg atcgcttgcg tcaatgattg gggattgaga atgtcttttg gtaccagtgt
     1261 ggtggtcacg gtttttgtgg gagctgtttt atttatttgt tgactcgtat ttggtttggt
     1321 cgccagactg tcccagacca aagtacaaga cacaataaac gacaggatga agatgagtgt
     1381 tttcatcata tgttctcatc gttatcttgc aagatgcgtg ccgttgaacc ctcaaaataa
     1441 atggagataa ttagttgata tgagtgggga ttgttttggc tagggagtgg ttcaacggat
     1501 tcgcgttatc aaatagggta gattggctgg ttcgttctca aaatgaaaaa tgtacctggc
     1561 acctgatttt tgctggtacc tgatttttgc tctggcatac caagccccca gcgttcagta
     1621 tcatcaagcc gcagaataac gggcaaagcc cct
//
LOCUS       DATDUN010000666         9503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_285666, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000666 DATDUN010000000
VERSION     DATDUN010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285666"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..418
                     /locus_tag="VJC18_09265"
     tRNA            348..418
                     /locus_tag="VJC18_09265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:380..382,aa:Gly,seq:ccc)
     gene            complement(684..1220)
                     /locus_tag="VJC18_09270"
     CDS             complement(684..1220)
                     /locus_tag="VJC18_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HLD45613.1"
                     /translation="MNEILSNQKDPQQLVTLAQIENQILLIRNQKVMLSNALANLYGV
                     KTKALIQAVKRNIERFPADFMFQLDNEEFNALKKYFPNVPWGKRANPYAFTQEGVAML
                     STVLNSKRAIQVNIEIMRVFVRFRQMLASNEDLKRKLLSLEKRYDLQFKAVFDAIRDL
                     MAPPIKHRKQIGFIVSKA"
     gene            complement(1428..1922)
                     /gene="coaD"
                     /locus_tag="VJC18_09275"
     CDS             complement(1428..1922)
                     /gene="coaD"
                     /locus_tag="VJC18_09275"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HLD45614.1"
                     /translation="MNKKIKNSAICPGSFDPPSNGHVNILLRSLNVVDHLVIAIGVNN
                     AKGCTFTVEERIGMLKEIFKDEPRIEIDSFSGLLIDYAKKKGIKTIIRGIRTMGDYEY
                     EFQMAQTNRKLNPDIDTIFMVTEGKYSYISSSIVKQVVSLGGCVKQMVPPIVERALRK
                     KLKP"
     gene            complement(2214..2780)
                     /gene="rsmD"
                     /locus_tag="VJC18_09280"
     CDS             complement(2214..2780)
                     /gene="rsmD"
                     /locus_tag="VJC18_09280"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HLD45615.1"
                     /translation="MRIIAGTKKNQKILTPKGTITRPALAQVREAIFSSLGNVDDLVF
                     MDIFAGCGSLGLEALSRNACYCYFVDNHPLALTCIINNLERLSFQNKAHVFKRTMPFG
                     LKNFNLEHKPHVIFCDPPYDQNLIDKTLNALLKYGFVGPETLVLVEHTRREVPSAQGW
                     HMTKQKKFGQTIISYLKLTVRSLPENLS"
     gene            2876..3394
                     /locus_tag="VJC18_09285"
     CDS             2876..3394
                     /locus_tag="VJC18_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HLD45616.1"
                     /translation="MKKSLLIFIMLGLIFSQCSATASKRSLGETLDDNVIVVKLKTRF
                     IKDKGVASNKIHIRSWKGVVTLSGALSQQEQIDRAIELAEQQGGVSEVKSHLVVATFS
                     PPKTKKSSPWPFSASNATRSKMKHKKETVIEEDLSETKEVAKKPDQNTAKNVGSEGLP
                     EKQNEDYKEFEY"
     gene            3403..3999
                     /locus_tag="VJC18_09290"
     CDS             3403..3999
                     /locus_tag="VJC18_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45617.1"
                     /translation="MKKFLCMTVIVFCVFSVSASVFADSKTYVFRKRAEWVKLVKLSN
                     KQMGGQVLLHPHTAVSVKTMTDILSGLKMNKAQGLKKGFKTNEVFSSEEAQKYAPFIV
                     QALGQAGPNQVVNVSVVHKRPYFMVRHDYLSLINVYVKDDGIHFYFSKLFARLDGDYE
                     QASRIYESIRKAKSLRVSLAPQSGQIISSDGNELVVRP"
     gene            complement(4023..4295)
                     /locus_tag="VJC18_09295"
     CDS             complement(4023..4295)
                     /locus_tag="VJC18_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HLD45618.1"
                     /translation="MNEGIIEKIKIVNKLGLHARAAAKFVKMAVLYKSQVSLGNDTDT
                     VDGKSIMDILLLAAENGSQVTLHVAGEDQAEAFASLKALIEGGFGE"
     gene            complement(4297..4704)
                     /locus_tag="VJC18_09300"
     CDS             complement(4297..4704)
                     /locus_tag="VJC18_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45619.1"
                     /translation="MVGVIIISESNACKEMLKTVGKVLNRDRLPGIYPLVLDASYTIE
                     ERLNKIQALINKADVGQGVLIMTELYGASQTNVCKHFLKGNHVKVLCGYNLPMIIEAV
                     TLNESAALGDICNLTAEVGKKYIRCPACEQNCT"
     gene            complement(4767..5633)
                     /gene="rapZ"
                     /locus_tag="VJC18_09305"
     CDS             complement(4767..5633)
                     /gene="rapZ"
                     /locus_tag="VJC18_09305"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HLD45620.1"
                     /translation="MSHKSKKQLLIISGYSGGGKSVALRYLEEIGFYCVDNLPARLLP
                     LFTSLLLINKNIFKIAIVIDARGKDFMTDLEEDLSFVNKHFETRIIFFDCSLQTIIKR
                     FKERRLKHPIALHGTIIEGYRKEQRLLTHLRQHATLVINTSQLNIHALKFELHKYLLK
                     ARDDFFEITLISFGYKFGLPEEADVVIDARLLQNPYFEPKLKKLTGQNKKVQHYIFDN
                     KMGRSFLAKTISFISFLANHFNKASKANLTIAIGCTGGKHRSVATAEKVRLAIKQKHK
                     FVNLYHRDVSKK"
     gene            complement(5646..6104)
                     /locus_tag="VJC18_09310"
     CDS             complement(5646..6104)
                     /locus_tag="VJC18_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HLD45621.1"
                     /translation="MKLGDLLKENALIPNLSAHDKKGVLVEFAERAQAINPVLKKETV
                     FQILHNREDLGSTAVGNGVAIPHGKVPGLDSIIAIFGRSEEGIDFQAHDEKPTHLFFV
                     LLAPESAIGNHLQALARLSRLLKESHIREKLVNCPQEELYDFLLAEDEKI"
     gene            complement(6237..6611)
                     /gene="raiA"
                     /locus_tag="VJC18_09315"
     CDS             complement(6237..6611)
                     /gene="raiA"
                     /locus_tag="VJC18_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HLD45622.1"
                     /translation="MQISITFRHMEATQAIRDHIDDKIGHLEKYLLNPTDIHVVLSVE
                     KFRHKCEVILMEQNLKASADETTDDMYKSIDKCVDKIESQVKKHKKIAQSHHKSKFSM
                     HDIAALAEKEYEKEITNKEEPN"
     gene            complement(7144..8631)
                     /gene="rpoN"
                     /locus_tag="VJC18_09320"
     CDS             complement(7144..8631)
                     /gene="rpoN"
                     /locus_tag="VJC18_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HLD45623.1"
                     /translation="MAIELKQSLKLTQQLLITPQLQQAIKLLQLSRTELIETVQKELL
                     ENPVLEEAPDSDSPEKTQTELAEAQTDNPTHSNEAFDKVDNSPLSNKEFDWGSFVENF
                     ATMGSREPRSLVSTEEQPNYENMVSQATSLQDHLEWQIKMGSLTAEEEEACFTLIGNI
                     DDNGYLQSSLEDLAEKTPFSYDELEDSLCIVQELDPPGVGARDLKECLLLQVKEYGPD
                     RSILTTIINKHLNLIEKRNFAALSKKLNLTTRKTRELADVIYALEPKPGRAYSTGTPQ
                     YITPDVYVQKVGEEYVVILNEDGLPKLQISNLYKTAIMREIASSKRNEKNPLNDEAKN
                     YIQEKLKSALWLIKSIHQRQKTLYKVTKSIVSFQKDFFDKGVQGLRPLVLRDVAEEIG
                     VHESTVSRATNGKYVHSPQGIFELKYFFNTGLTNSSGGEDFANEAVKQLIKQYISTED
                     IKNPLSDQAIAAMLTQLNINIARRTVAKYREMMGILPSSRRKRVS"
     gene            complement(8784..>9503)
                     /locus_tag="VJC18_09325"
     CDS             complement(8784..>9503)
                     /locus_tag="VJC18_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005026794.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HLD45624.1"
                     /translation="ELKYIDSEELEKDNTPLGDYFEGCSGILVPGGFGHRGIEGKINA
                     VQFARENNIPFFGICLGMQLAVIEFARHVAGLADANSDEFNNKTKNPVISLMAEQKAI
                     TNKGATMRLGHYPCVIENNTFAHEAYGERKIFERHRHRYEFNLKYKEQLCKKGLVTSG
                     TSPDGLLVEIIEIKGHPWFLGCQFHPEFQSSPMKPHPIFADFIVASIQFARKKDWINK
                     KEPLNAVSKKRPGPKAKIKIV"
BASE COUNT         2350 a         2080 c         2316 g         2757 t
ORIGIN      
        1 tccctctttt ttctgccact cggcagcgct cgcctcggct tcggctcact aggccgttcg
       61 cctcagccaa ctgagctaca tccccaaaaa atttgctatc gcaaattttc gttattcgta
      121 attcgttgtt cgtaattcga aaaagcaaca cgatgtcaga aacaaacctt attagtcgcg
      181 cccctcatac cctcagttaa tataaaattc aaactgaaat tgaatttgat ggattattga
      241 caaaatgtct tgtcattccc tacacaaata caatcaactt gttttttaga caggattcat
      301 ttagaggcag gcccggttgt tggggcgtga cccaaaacaa ccaaggtgcg ggtgtgatgt
      361 agtggtagca tataagcttc ccaagcttag tgtgtgggtt cgattcccat cacccgctca
      421 aaaaaagtcc ccgtagggga catttttttg agcgcagtta atccgccttc gcagaatgcc
      481 acgggtttac ccgtggatga atgcgatggg taagcttcgg cgagattcct cccaactggc
      541 gtaccagcgg gtgccatggg cttgcccgtg gagctccaca atatgttcac aatcagcaat
      601 ttagtgtcca aacaaggctt gcggccagct ataagtcaca atctgtgact tatagacgta
      661 tgtatttata aatattaatc aattcaggct ttggaaacga taaagcctat ctgttttctg
      721 tgttttattg gtggtgccat caggtcacgg atcgcatcga acacagcttt aaactgcaag
      781 tcatatcgtt tttccaaaga caaaagtttt ctttttagat cctcattcga ggcaagcatt
      841 tgtctgaatc gcacaaagac tcgcatgatc tctatgttca cctgaatggc ccttttgcta
      901 ttgagcaccg ttgagagcat tgcgacccct tcttgtgtaa aagcataggg gtttgctctt
      961 ttgccccacg gaacattagg gaaatacttt ttcagtgcat tgaactcctc gttgtccaac
     1021 tgaaacataa aatcggctgg aaatctctcg atgttacgct taaccgcctg aatcaatgct
     1081 ttggtcttga ccccatacag gtttgccagt gcattgctca acatgacctt ttgatttctg
     1141 atcaagagga tttgattttc gatctgcgca agggtcacaa gttgctgcgg gtctttttgg
     1201 tttgaaagta tttcgttcat aaaacaggca atgacaatgt ttcattgctt aacgaaatcg
     1261 cttcaacctg acaagttttt acttgattgt gctgaaaact gcggggaagc ctctatctaa
     1321 tttccaagct tagtgtgtgg gttcgattcc catcacccgc tctcagtttc ccacccattc
     1381 atgcgggccc tgccgaggcc acaccgccga tgacagcgtg ttttttttca tggttttaat
     1441 ttttttcgca gtgcccgttc cacaatgggg ggtaccattt gtttcacaca gccacccaag
     1501 gagaccactt gtttgacaat ggaggaactg atgtaactgt atttgccctc ggtcaccatg
     1561 aagatggtgt cgatgtcggg attgagtttg cggttggtct gggccatctg gaattcgtat
     1621 tcgtagtctc ccattgtgcg gatcccgcgg atgatggtct tgatgccttt ctttttggcg
     1681 tagtctatca aaagaccgct gaaagaatcg atctcaatgc ggggttcatc tttgaagatt
     1741 tcctttagca tgccgatgcg ttcttccaca gtaaacgtgc aacccttggc attattgacg
     1801 ccaatggcga tcaccagatg atccaccacg ttcaaagagc gcaacaggat gttgacgtgc
     1861 ccgttgctgg ggggatcgaa actgccggga caaatggcac tgttttttat ttttttattc
     1921 ataattactc ctaaagcgtg gagccaaaaa tatactcgta ttacgttttt ttgggtcccc
     1981 tcctacgcca aggcttcggc gggcaggctg ctccgggtcg gggtcctgaa tatcgaatat
     2041 cgatcaggga atctcgaatg tcgaagtgag gttttcttgt catcccagcg catgctggga
     2101 tccatgttgt tttctggatt cctgctttcg caggaatgac atatttcgac attccttgtt
     2161 ctctattcat tattcgatat tcaaatagct tcccgtatca cccaaaaaaa ctatcatgac
     2221 agattttcgg gcagactcct aacagtgagt ttcaaataac tgattatggt ttgtccgaat
     2281 tttttttgtt tggtcatgtg ccacccttgt gcggagggca cttcgcgtcg tgtgtgttcg
     2341 accagcacca acgtctctgg gccaacaaaa ccgtatttta acagggcatt gagtgttttg
     2401 tcgatcaggt tttgatcgta cgggggatcg caaaaaatca catggggttt gtgttcgagg
     2461 ttaaagtttt tcaatccaaa gggcatggtg cgcttgaata cgtgcgcctt gttttggaag
     2521 ctcaaccgtt ccagattgtt aatgatgcac gtcagggcca gtgggtgatt gtctacaaaa
     2581 tagcaatagc atgcgttgcg gctcaaggcc tcaagcccca gactcccgca accggcaaaa
     2641 atatccatga agaccagatc atcaacattg cccagacttg aaaaaatggc ctcgcgcacc
     2701 tgcgccaagg cagggcgggt gatcgtgccc ttgggggtga ggattttttg gttttttttt
     2761 gtgcccgcta tgattctcat gaatacgcaa tacgcaatac gctgtacgct gtacgctgta
     2821 cgtgcgcctg cgatattggc ttgtcaaagg tttggcacca tcttataagg cctttatgaa
     2881 aaaaagtctg ctaatattca tcatgcttgg cctgattttt tctcaatgtt ccgccacggc
     2941 aagcaaacgg agtttagggg aaacgcttga tgataatgtg atagttgtca aactgaaaac
     3001 caggttcatc aaggataaag gtgtggcatc aaataaaata catatccgtt cgtggaaagg
     3061 ggtagtgacg ctctctggtg ctttgagtca gcaggagcaa attgatcgag ccatcgaatt
     3121 ggccgagcaa caaggtggtg tgagtgaagt caaatcccat ctggttgtag caaccttcag
     3181 tccaccgaag acgaagaaat cttctccctg gccgttttct gcctcaaatg ccacgcgatc
     3241 caagatgaaa cacaaaaaag agacggtgat agaagaggac ctttccgaaa ccaaagaggt
     3301 ggcaaaaaaa ccagatcaaa acaccgccaa gaacgttggt agtgaggggc ttccggaaaa
     3361 acaaaatgag gattacaagg aatttgaata ttaaggatta ttatgaaaaa gtttttgtgt
     3421 atgactgtga ttgttttttg cgtcttttct gtgagcgcgt ctgtttttgc cgatagtaaa
     3481 acatatgttt ttcgcaaaag ggctgagtgg gtcaaattgg tcaaattgtc gaacaaacaa
     3541 atggggggac aggttttgtt gcatccacac acggctgttt cggtcaaaac catgaccgac
     3601 atcctgtcgg ggctaaagat gaacaaggca cagggcctca aaaagggatt caaaaccaat
     3661 gaggtttttt cgagcgagga agcccaaaaa tacgcgccgt tcattgtgca ggcactgggt
     3721 caggcggggc cgaatcaggt ggtgaatgtg tcggtcgtcc acaaacgccc ctattttatg
     3781 gtcagacacg attatctgtc tctgattaat gtatacgtca aagacgacgg gattcatttt
     3841 tatttttcca aattatttgc cagattggac ggcgattacg aacaggccag ccggatctac
     3901 gaatccattc gcaaggcgaa aagcctgcgt gtgagcctgg cccctcagtc cggacaaatc
     3961 atctcgagcg atggcaacga gctcgtcgtg agaccctagg agtgggatgg tttcatcccc
     4021 gctcactcac caaacccgcc ctcgatgagg gccttcaagg aggcgaacgc ctctgcctgg
     4081 tcttctccgg ctacatgcaa cgtgacctga ctgccatttt cagcggccag cagaaggatg
     4141 tccatgatgg atttgccatc gacggtgtcg gtatcgttac ccagggaaac ttgggatttg
     4201 tacaagacgg ccattttgac aaacttggcc gcagccctgg cgtgaagacc gagtttattg
     4261 acgattttga ttttttcgat aatgccttcg ttcatatcat gtgcaatttt gctcgcacgc
     4321 aggacaacga atatattttt tgccaacttc tgcggtaagg ttgcaaatat cgcccaatgc
     4381 ggccgattca ttcagcgtga cggcctctat gatcatggga aggttgtagc cacacaacac
     4441 cttgacgtga ttgcctttga gaaaatgctt acagacattt gtctgcgaag ccccataaag
     4501 ctccgtcatg atcaaaaccc cctgacccac atcggccttg ttgatcaggg cctggatttt
     4561 gttcagccgc tcctcaatgg tgtaggaagc gtccaggacc aagggataga tccctggcag
     4621 acgatctctg tttaacactt tgccaactgt tttgagcatt tctttgcagg cgttgctttc
     4681 tgagatgatg atgactccaa ccataaactt gtcccaagcc gaatagcggc aaaatagact
     4741 tcttttcccc tcccacgtca aggggattat tttttggaca catcccgatg atacagattg
     4801 acgaacttat gcttttgctt gatggccaag cggaccttct cggctgtggc aacggatctg
     4861 tgtttgccgc cagtacaccc gatggcgatg gtgaggttgg cctttgaagc cttgttgaaa
     4921 tgattggcca gaaaagagat gaaggagatg gttttggcga ggaaagatct gcccattttg
     4981 ttgtcgaaaa tgtaatgttg aacctttttg ttttgaccag tgagtttttt gagtttgggt
     5041 tcgaagtacg gattttgcaa cagccgggca tcaatgacca catctgcctc ttcgggaagt
     5101 ccaaatttgt aaccaaagct gatgagtgtg atttcgaaaa aatcatcacg tgccttaagc
     5161 aaatacttgt gcagttcgaa cttgagggca tggatgttga gctgagaggt gttgatgacg
     5221 agcgtggcgt gttgtctgag gtgcgtcagc aaccgctgtt ccttgcggta gccctctatg
     5281 atggtgccgt gaagcgcgat ggggtgtttc aggcgccttt ccttgaaccg cttgatgatg
     5341 gtttgcaggg aacaatcgaa aaaaatgatc ctggtctcaa aatgtttgtt gacgaaagaa
     5401 agatcctctt cgagatctgt catgaaatcc ttgcctctgg catcaatgac gatggcaatt
     5461 ttgaagatgt tcttgttgat gagcagaaga cttgtgaaca acgggagaag cctggcaggc
     5521 aagttgtcga cacaatagaa accgatttct tccaggtagc gcagagcgac cgatttgcca
     5581 ccaccagaat aacccgagat gatcaacagt tgtttttttg atttgtgcga catgaaaaca
     5641 acccgttata ttttttcgtc ctcagcgagg agaaaatcat agagttcttc ttgagggcag
     5701 ttcacaagtt tttcgcgaat gtggctttct ttgagaagac gggaaagcct ggccaaagcc
     5761 tgcaggtgat tcccgatggc tgactcgggg gcaaggagta caaaaaataa atgggttggt
     5821 ttttcgtcgt gggcctgaaa atcaatgccc tcttcggacc tgccaaaaat ggcaatgatg
     5881 ctatccaacc ccgggacttt tccgtgtggg atagccacgc cgttgccgac ggctgttgac
     5941 cccaagtctt cccggttgtg caatatttga aagacggttt cttttttgag gacggggttt
     6001 atggcttggg cgcgttccgc gaactcaaca agaacgcctt ttttgtcgtg ggcggacaaa
     6061 ttgggaatca aagcattttc ttttagcaaa tcacctaatt tcatgggacg tccctactaa
     6121 aactttgccc tacataacgc aaaggatttt cagcttgcaa ccacaaaaaa gggctttcaa
     6181 aaaagccctg agattaaaat aaatgggtgt tcaatggttt tcagaagtcc ccttaattaa
     6241 tttggttctt ctttatttgt gatttctttt tcgtattctt tttctgccaa ggcggcaata
     6301 tcgtgcatgg agaatttgct tttatgatga ctctgcgcga tttttttgtg ctttttgacc
     6361 tgactttcga ttttgtcgac gcatttatcg atcgatttgt acatgtcgtc ggttgtttca
     6421 tcggcactgg ccttgaggtt ttgttccatg agtatgactt cgcacttatg acgaaatttt
     6481 tccacggaca gcacgacgtg tatgtctgtg ggattaagta aatatttctc cagatggcct
     6541 attttgtcat cgatgtgatc acgaattgcc tgtgtggcct ccatgtgtct gaatgtaatt
     6601 gaaatctgca tcatattctc ctacatcaag cgttactaaa aaaacctttt atattgaata
     6661 gttaagaggt ctttgaagcg tgttgaatca tacgaacaag gtcggactcc taataaaaag
     6721 cacatggttt gtcacccaca ttttttacaa aatgtaacgt atggaaatga tgggggaatg
     6781 aaaggggcaa tgaaacaatc aagaaatgaa gcaatgaagc aatgaagcaa tgaggcactg
     6841 gttatcgtat agggcatctc taataaccat gttcgtcacg aaaaattaca atctgagtcg
     6901 aaacaaggac gataagcaaa aagcccgcag acgtgtaagg cacgccacgt tgaggacttt
     6961 ttgtgaaatc ggacgcagtt gcagaccaga gtgtaatttt gcagtagaag atggttatga
     7021 ggtgccctat atctaaatca actgaaaggc taacagctgg tactctcagg ggcacaagcg
     7081 gcctacaatt gacagtctga aaaatccagc cgttggctgt tggccgtcga ctgttgaccg
     7141 aattcaagat acgcgtttac gtcgtgagga gggtaatatt cccatcattt cccgatattt
     7201 ggccacagtt ctgcgggcaa tgttaatgtt gagttgtgtg agcatggccg cgatggcctg
     7261 gtctgacaaa gggtttttga tgtcctcggt actgatgtat tgtttgatca actgcttgac
     7321 tgcctcgttg gcaaaatcct caccgccgct tgaattggta agccctgtat tgaaaaaata
     7381 cttaagttcg aaaatgccct gaggggaatg aacatatttt ccattggtgg cgcggctgac
     7441 cgtggattcg tgaacgccga tttcttcggc aacgtctcgc aaaacgagtg gtctgagtcc
     7501 ttgcacgccc ttatcgaaaa aatctttctg aaagctcacg atggatttgg taaccttgta
     7561 caatgttttt tgtctttgat gaatgctctt gatgagccac agggcgctct tgagtttttc
     7621 ctgaatgtag ttttttgcct catcgttgag ggggtttttt tcgtttcttt tggaggacgc
     7681 gatttcgcgc atgatggccg ttttgtacaa attgctgatt tgcagttttg gcaagccgtc
     7741 ttcgtttaaa atcaccacat attcttcgcc cactttttgc acataaacgt cgggagtgat
     7801 gtactgaggg gtgcccgtgc tgtaagcgcg gccaggcttt ggctccaggg catagatgac
     7861 gtcggccagc tctctggttt tgcgtgtggt gaggttgagc tttttgctca acgcggcaaa
     7921 attgcgcttt tcaatgaggt tgaggtgttt gttgatgatg gttgtcaaaa tggaacggtc
     7981 gggaccgtat tctttgacct gcaggagaag gcattccttg agatcacgcg cgccaacgcc
     8041 gggagggtcg agttcttgca cgatgcacaa agagtcttcg agttcgtcat aactgaacgg
     8101 tgttttttcg gcgaggtctt cgaggcttga ttgcaagtag ccattgtcgt cgatattgcc
     8161 aatcagtgtg aaacaggcct cttcttcctc ggctgtgagg ctacccatct tgatttgcca
     8221 ttccagatgg tcctgaagcg aggtcgcctg ggacaccatg ttttcgtaat tgggttgttc
     8281 ctctgtgctg accagagaac gcggttcgcg acttcccatg gtggcaaaat tctcaacaaa
     8341 actgccccaa tcgaattctt tgttcgaaag agggctgttg tcgaccttgt cgaatgcctc
     8401 gttggaatgg gtgggattgt ctgtttgggc ctcagccaac tctgtctgtg ttttttcggg
     8461 agagtccgaa tcgggggcct cttcgagaac agggttttct aacagttcct tttgaacggt
     8521 ttcgatgagt tctgttcggg aaagctgtaa taatttgatg gcttgttgga gctgtggcgt
     8581 gatgagcaat tgctgggtga gcttgagtga ttgcttgagt tcaatggcca taatccactc
     8641 ccctaatgtt gcgataatac cctgaaaacg actgatttag ggctccacat gcaaatacca
     8701 taccattgta agaggtgatg attggcatgt caattgctaa ttgactcccg tgtgcaaaaa
     8761 aaacaatcaa ccaattataa ttattaaaca attttaattt ttgcctttgg gccaggccgt
     8821 ttttttgata cagcgtttag gggttctttt ttatttatcc aatctttttt tcgtgcaaac
     8881 tgaatactgg ccacaataaa gtccgcgaaa atgggatgtg gtttcatggg actggattga
     8941 aactcgggat gaaactgaca tcccagaaac cagggatgtc ccttgatttc gataatttca
     9001 accaagaggc cgtcggggga tgttccagag gtcacaagcc cttttttaca cagttgctct
     9061 ttgtacttaa ggttaaattc ataacgatgt ctgtgtctct caaaaatttt acgttcgccg
     9121 taggcctcgt gggcgaaggt gttgttttcg atcacgcagg ggtagtggcc aagccgcatg
     9181 gtggcacctt tattggtgat cgccttttgc tctgccatga gactgatgac aggatttttg
     9241 gttttgttgt tgaattcgtc tgagttggcg tcggcaagtc ctgccacatg tcttgcaaac
     9301 tcaatgacag ccagttgcat ccccaagcag attccaaaaa aaggaatgtt gttctcgcgt
     9361 gcaaactgga cggcattgat cttgccctcg atcccccgat gaccaaaacc acccgggact
     9421 aaaatacctg aacacccctc aaaatagtct cccagcgggg tgttgtcttt ttcgagttct
     9481 tcggaatcga tgtatttgag ttc
//
LOCUS       DATDUN010000667         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_155109, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000667 DATDUN010000000
VERSION     DATDUN010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155109"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1744)
                     /locus_tag="VJC18_09330"
     CDS             complement(65..1744)
                     /locus_tag="VJC18_09330"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HLD45625.1"
                     /translation="MVRTNPTIAKNPAVDKDSVKSNLKYLQFGIRLWTLRERQKIRSK
                     ILADKLGIPFHEYRRYEAGEILPDQDTLQQLAQILKVELSQLTQWKSKELHQSGKPAM
                     AKGAHKYLYHWDLAEQQFRKNWLSHHEKEQRHEHIIHEIIILLKKESCSLKTLPILPL
                     GLYLVLDALISNQHHDKKFIHDISDFVVENETLAQYMARDPVLGCFLFVAANRVYFSK
                     APLDGIEDCFNRLTVSQFSDLVFMATTPEGVHANYETLPSLQRFIEFSSLAVLMVREL
                     KDYLPDNVNYHHLYLAALLQGVGTYALHTSLQTMIDQHPEWYVVTKPELAEDVHCKKS
                     LAQHLNWEFHPIISGMIAANWGLPHEVVQTLIEHHKQPTNEVSPVCAVLKLINFFVDC
                     DFPRIKANEIQDLLAKYPQVSIDPQALLTVSQHMNKMSEYLIEMNSVVLDNTQVTSLG
                     SATQKEIEILDAGADPICSKPVWRHQPSAKTEFRFDTEYSKILTIDCSQHLNQFLTQH
                     LSPKKGESLRGYGERVQNLQLALYLALSGDLADVSARFQLSEQEIALRLSL"
     gene            complement(1823..>2240)
                     /locus_tag="VJC18_09335"
     CDS             complement(1823..>2240)
                     /locus_tag="VJC18_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD45626.1"
                     /translation="QQYTHAIRDYTIAINLNDQSLWPYIGLARVYLKQKQYKHAKEIL
                     QMGQEQLPLNGALDFYLGHVANATKQPELAVQHYTDAQEHAYPRADKIHYYRGLVYQN
                     QLKDHAQAKQDFESFLKSNTTDTAQIAEVKQHLQSL"
BASE COUNT          480 a          481 c          650 g          629 t
ORIGIN      
        1 ttgatgaggc tctcgatgtc gatatccgac aagccagaga agtggcaatg tgtgtcgatc
       61 agtgtcataa gctcaacctg agcgcgattt cctgttcgga aagttgaaac cgcgcggaga
      121 cgtccgccag atcgccgctc agggccagat acaacgcaag ttgcaggttc tgcacacgct
      181 cgccgtaccc cctgagcgat tccccttttt tgggggagag gtgttgtgtg aggaactggt
      241 tcaggtgttg cgaacagtca atggtgagaa ttttgctgta ttctgtgtcg aaccggaact
      301 cagttttggc agacggttgg tgacgccaga cgggtttgga acagatgggg tctgctcctg
      361 cgtcgaggat ctcaatttct ttttgagttg cactgcccag agaagtgact tgcgtgttgt
      421 ccaacaccac ggagttcatc tcgatgaggt actcactcat cttgttcatg tgttgagaca
      481 ccgtgagcag ggcttggggg tcgatggaga cttgcgggta cttggccaag agatcctgaa
      541 tttcgttggc cttgattcgc ggaaagtcgc agtccacaaa aaaattgatg agcttcaaaa
      601 cggcacagac aggggacacc tcgtttgtcg gttgtttgtg gtgttcgatt aaggtctgca
      661 caacctcgtg cgggagcccc cagttggcgg cgatcatgcc actgatgatg gggtgaaatt
      721 cccaattgag gtgctgagcc agtgattttt tgcaatgcac gtcttccgcg agttccggtt
      781 ttgtaaccac ataccattct ggatgttgat cgatcatggt ttgcaatgat gtgtgcaggg
      841 cgtaggtgcc aaccccctgc aggagagcgg ccaggtagag gtggtgataa ttcacgttgt
      901 ctggaagata gtctttgagt tctctcacca tgaggactgc caaactggaa aattcgataa
      961 agcgttgcag ggaaggaagt gtttcgtagt tggcgtgcac accctcaggt gtggtggcca
     1021 tgaacaccag gtcggaaaat tggctgacgg tgagccggtt gaaacaatcc tcgatcccgt
     1081 ccaggggggc tttcgaaaaa tagacgcggt tggccgccac aaataaaaaa caccccagca
     1141 caggatcccg ggccatgtac tgggccagag tttcgttttc gacgacgaaa tcggaaatgt
     1201 cgtggatgaa tttcttgtcg tgatgctggt tggaaatcag cgcgtccaat accagataca
     1261 gtcccaaagg caggatgggc aatgttttga gggaacagga ttcttttttg agaagaatga
     1321 taatttcgtg aatgatgtgt tcgtgccgtt gctctttttc gtgatgggac aaccagtttt
     1381 tacggaactg ctgttccgcc aggtcccaat gatacagata tttgtgtgcc cccttggcca
     1441 ttgcgggttt tccggattgg tgcagttctt tgcttttcca ttgggtgagt tgcgacaatt
     1501 ctacttttaa tatctgtgcc agttgctgaa gggtgtcttg atcgggcagg atttccccgg
     1561 cttcgtaacg acggtattca tgaaaaggga ttcccaattt gtcggccagg atcttgctgc
     1621 ggattttttg tctttccctt aaagtccaca agcgaatgcc aaactgcagg tacttcaggt
     1681 tcgatttgac agagtccttg tccaccgcgg gatttttggc gattgttgga tttgttctga
     1741 ccatgacggg tcctccacgt aggtattggg atgaactcct aacaagatat tttgtgactg
     1801 tcactgaatt gttgcgtgat cctcacagcg attgcaagtg ttgtttgacc tcggcaattt
     1861 gggcggtatc ggtggtgttg cttttgagaa aggactcaaa atcctgtttg gcttgggcgt
     1921 gatctttgag ctgattctgg taaaccagac cgcggtaata gtggatcttg tctgccctgg
     1981 gataggcatg ttcctgggca tcggtgtaat gttggactgc caattcaggt tgtttggtgg
     2041 cgttggcgac gtggcccaga taaaaatcga gtgcgccgtt caaggggagt tgttcctgac
     2101 ccatctgcaa aatttctttg gcgtgtttgt actgcttttg cttgagatac acacgggcca
     2161 gaccgatgta tggccagagg ctctggtcgt tgaggtttat ggcaatggtg taatcccgga
     2221 tcgcgtgggt gtattgctgc
//
LOCUS       DATDUN010000668         1713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_285724, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000668 DATDUN010000000
VERSION     DATDUN010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285724"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..386)
                     /locus_tag="VJC18_09340"
     CDS             complement(87..386)
                     /locus_tag="VJC18_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45627.1"
                     /translation="MKTRNVLFWIFVLFTVTLCTVTLAGSHQRNTTQLKNIEQLVLIT
                     QHLDLESRALVDINEKQPERSCFCACDDYSSRWICTTKECKSHDKTCSNEIDENR"
     gene            complement(521..1243)
                     /locus_tag="VJC18_09345"
     CDS             complement(521..1243)
                     /locus_tag="VJC18_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HLD45628.1"
                     /translation="MIETNKPLIIAHRGASSLEPENTMRAFHKACELGADGIELDIFL
                     TKDNQLVVTHDRDTQRLTGISFDVCQSHYHKLSTLDYGKGEQLPLLSDVLEEFKNKFH
                     VINIEIKSTGLFTNGVETVLAQLLNKYSVFDRIIVSSFNPLHLLRLKKILPQTRVACL
                     ISQHQHPLARHPFVIKLLRPTTLNLDHRLEHDKHFTHFFSGPIKKWVWTVNEPADLDH
                     WLTQDVDAIITNDPKLLLSLLE"
     gene            complement(1236..>1713)
                     /locus_tag="VJC18_09350"
     CDS             complement(1236..>1713)
                     /locus_tag="VJC18_09350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLD45629.1"
                     /translation="ELFTVKGAGSLIQLGSHIQMLSSLDQVDTHSLQKLLEISLEKTI
                     SPIFFRNKFDHIYLEENYLGVALLRNYQDMAYLMAFAVGTEARGLGIGKDLWKEICNH
                     HKRFFWRSKPDKFITHWYTKQCDGMHRTPEWTVFWRGLRPNEVSQAIEYAIGQADD"
BASE COUNT          420 a          381 c          449 g          463 t
ORIGIN      
        1 aggctcgttc gacccactgc tgtgggtgcg attgggaata atagcttcgc cagtcacccg
       61 tacgaaatgt cctcacaaga tggcatttac ctattttcgt caatttcatt cgagcacgtc
      121 ttgtcgtgcg atttacattc cttggtggtg cagatccaac gtgagctata atcatcacag
      181 gcgcaaaagc aggagcgttc cggttgcttt tcgttgatgt cgacgagcgc gcggctctca
      241 agatcgaggt gctgtgtgat caggaccagt tgttcgatgt ttttgagctg tgtggtgttt
      301 ctttgatgtg accccgccag ggtgactgtg cacagggtga cggtgaacag aacaaaaatc
      361 caaaacagaa cgttgcgtgt tttcataaaa tgggcctttc gcctatacac cagcaagatt
      421 caggccagaa tcccgcagag aaaccaataa tcatttcgtg ttgttaaaat aattgatgag
      481 ggaaatgatc aaaacaaccg tcaaagacct gacgaaggga tcattctaat aaactcaata
      541 acagctttgg gtcattggtg atgatggcgt cgacatcctg cgtgagccag tgatccaggt
      601 ccgcaggctc gttcactgtc cacacccatt ttttgatggg tccagaaaaa aaatgggtga
      661 agtgtttatc atgctccagc cggtgatcga ggtttagcgt cgttggcctg agcagtttga
      721 tcacgaaggg gtggcgggcc agaggatgtt ggtgttgaga aatgagacag gccactctgg
      781 tttgaggcag gatttttttg agccgcaaca ggtgcagtgg gttaaaactc gaaacaatga
      841 tccggtcaaa tacagagtac ttattgagca gttgtgccaa cacggtttcg acgccatttg
      901 tgaacagccc cgtggatttg atctcgatat tgatgacgtg aaatttgttt ttgaattcct
      961 ccaaaacatc agaaagaaga gggagttgtt ccccttttcc ataatccaga gtgctcagtt
     1021 tatgataatg gctttgacat acatcaaacg aaatccccgt gagtctttgc gtgtcgcggt
     1081 cgtgtgtgac cacaagctga ttgtcttttg tgagaaagat gtcgagctca atgccatcgg
     1141 cgcccagttc acaggctttg tgaaaggccc gcatggtgtt ttcgggttca agagagctgg
     1201 ccccgcggtg ggcgatgatg aggggtttgt ttgtttcaat catccgcttg accaatggcg
     1261 tattcgatgg cttggctcac ttcgtttggg cgcaaacctc tccagaacac ggtccattcc
     1321 ggcgtccggt gcatgccatc gcactgtttg gtgtaccagt gagtgatgaa tttatccggt
     1381 tttgaccgcc agaaaaaacg tttgtggtgg ttacagattt ctttccaaag atccttgcca
     1441 atgcccagcc ccctcgcctc ggtgccaacc gcgaaggcca tcaaataggc catgtcctga
     1501 taattcctga gcagggccac ccccagataa ttttcttcca ggtaaatgtg atcgaatttg
     1561 ttacgaaaaa aaatcggtga gatcgtcttt tcgaggctga tttccaaaag tttttgaagg
     1621 gaatgagtgt ccacctgatc gaggctggac aacatctgga tgtgggatcc caactggatc
     1681 agagagccag cgccttttac ggtaaagagt tct
//
LOCUS       DATDUN010000669         1296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_236314, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000669 DATDUN010000000
VERSION     DATDUN010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236314"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VJC18_09355"
     CDS             complement(<1..213)
                     /locus_tag="VJC18_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45630.1"
                     /translation="MFKIVILFISLFLSTSSYAYYGCTSGTNTAGVENVGPPGWLAYT
                     CGGVATEFCHMGRCEDGFDPNKKGGDP"
     gene            complement(263..487)
                     /locus_tag="VJC18_09360"
     CDS             complement(263..487)
                     /locus_tag="VJC18_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45631.1"
                     /translation="MNKNIIRGLVASGVLVATGAQAALDASIGTAITDGVTDILAVIA
                     LGGAAYITISGAGVIWNVASKFVKRLGGKA"
     gene            complement(909..1232)
                     /locus_tag="VJC18_09365"
     CDS             complement(909..1232)
                     /locus_tag="VJC18_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLD45632.1"
                     /translation="MSALPTEINKSRPSLRPMEVLISGRIEEVQRYEGTYQTRVICPA
                     ADQFSQPQNVKIRSKQRLGSKEEMISVTCRLGGYKRKVFRATDKETGESVSVVPVDMT
                     LDLVD"
BASE COUNT          389 a          337 c          304 g          266 t
ORIGIN      
        1 aggatcaccg ccctttttat tgggatcaaa accgtcctca catcgaccca tatggcaaaa
       61 ctcagtagcg acaccaccac acgtataagc cagccaaccg ggagggccaa cattttcaac
      121 cccggccgta tttgtgccag atgtacagcc gtaataggca tacgaagagg tagataaaaa
      181 taacgatata aacagtatta ctattttgaa cataggaaaa tcctttgcga tgcactcccg
      241 agggagtgca tgaaaaaggc ctttacgcct tgccgcccaa acgcttgaca aatttggatg
      301 ccacgttcca aatcacgcca gcccctgaaa tcgtgatgta agcagcacca cccaaggcaa
      361 taacagcgag gatgtcagtg acaccatcag taatggccgt accgattgac gcatccagcg
      421 cagcctgagc gccagtagca accaagacac cggaggccac caggccgcga atgatgtttt
      481 tgttcatcga aaaaactcct ttaaaaacac tcggaaaacg ccgagcacgt atcaggacgc
      541 acgccccaaa tctttgagaa ttcgcacata tttaccccac tgataaccgg caaaatagac
      601 gatgtaaagg gacccaacga agtagctcac aaactctaag tcggtcatga tgagagcccc
      661 tggcgatagc cgagcatgcc agcgaagaac agcgcagaca cggcgatgac gactaaaaca
      721 agttgaatat cagtcattaa attcacctaa aaaatacgga tcaaccggcc caaaccgcga
      781 aggcaaatcg aacgacatat aagcggcaca ggacgataaa cacagaatga gaagagagaa
      841 aaaaatcgtt ttcatgattg atttttggcc tggtgcgtcg acgcgtccgc gtctagcacc
      901 agtccaaatc aatccaccaa atcgagagtc atatcaaccg ggacaaccga gacactttcg
      961 ccggtttcct tgtcagtcgc ccgaaagact ttccgtttgt agccgccgag gcgacaagtc
     1021 accgagatca tttcttcttt tgatccaagc cgctgtttac tgcgaatctt gacgttttga
     1081 ggctgactga attggtcggc agcaggacag atgacacggg tttgataagt cccctcatag
     1141 cgctggactt cttcaattcg gcccgaaatg aggacctcca tgggacgcaa agagggacgg
     1201 gatttgttga tttcagttgg caatgcagac atgatgattc cttgaagtta cgcggctttg
     1261 aggtgtactg gcggcaggct ataccagtcc ggaggc
//
LOCUS       DATDUN010000670         1522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_286097, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000670 DATDUN010000000
VERSION     DATDUN010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286097"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1522)
                     /locus_tag="VJC18_09370"
     CDS             complement(<1..>1522)
                     /locus_tag="VJC18_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HLD45633.1"
                     /translation="EADSTQVDSLLAGIGSFLLVALLIFAGFRELRRPFFALLALMIG
                     MAWTTGFASLFFGQLNLITIAFVIILIGLGIEYGIHFLSRYLWERKEGHETTAAISKT
                     LEHTGRAIGIGGITAAAAFLTAIFTHFKGLQQLGIIAGAGVIFCFLAEVTILPSLLML
                     FDTHKQTNKTIPANKQVRFDFLVKHPKPTIIVLSVFTLIGLIVAPSLHFDSNLLNLQD
                     QKLESLVFEKIIQDNTEQSTWFLTYHTKDLNDLYDKQTRVAQLSSVKKTDSLFQLVPQ
                     NQKERLLQLQDLQKKLQHSHVPASPQNLPQQVDLLKTQVATLTNQAFKNGMIEEFNEL
                     NSLNDTLGKQSDTLRAAKRVPFENNFLTFVNRLQGELHSLLTPSLVTEAELPSKLLSL
                     YKSANGSYSLTIYPHKDIWDPQNQEEFINQVRTIIPDVTGAPVTTYESAKRLVSGFLL
                     VALLTSLIILFAIWAEFREIRASLFVFGNLVISLIWLVGYLYIRDLSINLANFFALP"
BASE COUNT          375 a          317 c          445 g          385 t
ORIGIN      
        1 tgggcagggc aaaaaaattg gccagattga tcgaaaggtc cctgatgtat agataaccga
       61 ccagccagat caggctgatg acgagattgc cgaatacaaa caaggaggcc ctgatttctc
      121 tgaactctgc ccagatggca aagaggatga tcagtgaggt gagcagggcg accagcagga
      181 aaccgctgac cagcctcttg gcggattcat aggtggtgac tggcgcgccg gtcacatcgg
      241 ggatgattgt tctcacttga ttgatgaatt cttcctggtt ctgggggtcc caaatgtctt
      301 tgtggggata gattgtcagg ctgtaagacc cgtttgctga tttgtaaagt gaaagcagtt
      361 tcgagggcaa ttctgcctcg gtaacgaggg agggggtcag tagggagtgt aattcgccct
      421 gtaacctgtt cacgaaggtc aggaaattat tttcgaacgg aacccgcttt gcagcgcgta
      481 aggtgtcgct ctgtttaccg agtgtgtcgt tgagggaatt gagttcatta aactcctcaa
      541 tcatcccatt cttaaaggct tgattggtga gtgttgccac ctgagtcttg aggagatcga
      601 cttgctgtgg gaggttttgt ggggaggcag ggacgtgact gtgttgcagt tttttctgta
      661 agtcttggag ctgtaaaagc ctctcttttt gattttgcgg gacaagttga aagagggaat
      721 cagttttttt tacggaagac aactgcgcga ctctggtctg tttgtcgtac agatcgttca
      781 ggtcttttgt gtgataggtc agaaaccagg tggattgctc cgtgttgtcc tgaatgattt
      841 tctcaaagac caggctttcc agcttctgat cctgcaggtt caataggttg ctgtcgaaat
      901 ggaggcttgg tgcaacgatc aaaccgatca aggtgaacac agacaacacg atgatggtgg
      961 gtttgggatg tttgaccaaa aaatcaaaac gaacctgttt gttggcaggg attgttttgt
     1021 ttgtttgttt gtgggtgtca aacagcatga ggagtgaggg gaggattgtg acctcggcca
     1081 gaaaacaaaa aataacgccc gcccccgcga tgatgcccag ttgttgcagt cccttgaagt
     1141 gcgtgaaaat ggcggtgaga aaagcggccg cggccgtgat gccgccaatg ccgatggctc
     1201 gtcctgtgtg ttccaaggtt tttgaaatgg ccgccgtggt ctcatgacct tcctttcgtt
     1261 cccaaaggta gcgggacaga aaatgaatgc cgtattcgat gcccaaacca atgaggatga
     1321 tcacgaacgc gatggtgatg aggttcagtt gcccaaagaa gagactggcg aaacctgttg
     1381 tccaggccat accgatcatc aacgccagga gggcaaaaaa ggggcgtcgc aattcacgga
     1441 accctgcaaa aatgagcaat gcgaccagaa gaaaagaacc gatccctgcc agcaggctgt
     1501 caacttgagt gctgtcggcc tc
//
LOCUS       DATDUN010000671         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_367271, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000671 DATDUN010000000
VERSION     DATDUN010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367271"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VJC18_09375"
     CDS             complement(<1..478)
                     /locus_tag="VJC18_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLD45634.1"
                     /translation="MNAIWTLAKKELKDLFGSPVAYVFVCITLFASFWLFFAQVFVVG
                     QTSLRALFDWLPVLFTIFLPSVTMGAWSGEKKSGTLEILLTLPVSETALVLGKFLASL
                     FFLGFLLVLTLPLPVVFAYLGDLDFGVVITSYAGSLLLGATYLAIGLWLSSLVKQQV"
     gene            complement(475..1383)
                     /locus_tag="VJC18_09380"
     CDS             complement(475..1383)
                     /locus_tag="VJC18_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLD45635.1"
                     /translation="MTKSFGNVRAVNNLSFDIATGEVVGFVGPNGAGKSTTMRVITGY
                     LAPSSGEVTINGLCVQQNPLKTKAQVGYLPESSALYDDMFTIDFLRFAGGMRGLDRKK
                     LKQQLSCVIEQCDIASILDRKMGTLSKGFRQRVGLAQAFLHDPPVLILDEPTIGLDPN
                     QVVQIRTLLKNIGKTKTVLLSSHILSELTQTCDRLLVIHNGSLVAQGTADELRQKTQE
                     QNQYHICLRGNLTDIENIIRACPFCLSLTVTSSQDDKHHVAVVCSDAHDHSEDFFQLA
                     VANNWQLNTLTKNQSSLESVFQELTQ"
     gene            complement(1398..>2065)
                     /locus_tag="VJC18_09385"
     CDS             complement(1398..>2065)
                     /locus_tag="VJC18_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD45636.1"
                     /translation="EGKYFFKTQKYKKAEELFKKVVAQKKEYADVYNCLGIISHEEGR
                     YGEAIKNFKKALQINPRYTEAMLNLSILHNDLGEYENAKKLVNRSRKDAKAKKAEMDP
                     FIRSKLANKHAEIADRYKGVGLYNEAILEYKKALELESYADIRTKMAVCLRENGNTKG
                     ALEELKKAIKTNKAFADIHIQLGITYYSLGKKDEAKKVWKAAEKLFPSNKTIKMYLRF
                     TDK"
BASE COUNT          503 a          474 c          541 g          547 t
ORIGIN      
        1 aaacctgttg tttgaccaga gatgacagcc acaggccgat ggccagatag gtcgcgccga
       61 ggaggaggct gccggcatag ctcgtgatga cgaccccaaa atccagatca ccaagatagg
      121 cgaagaccac ggggagtggc agtgtgagga ccaggagaaa gcccaaaaaa aacagcgaag
      181 ctaaaaattt gcccagaacc aaagcggttt cacttacggg cagggtgagc agaatttcca
      241 aagttcctga ttttttttct ccagaccagg cccccatggt cacagaaggg agaaagatgg
      301 tgaacaggac gggaagccaa tcgaataaag ccctgagact ggtttgtccc accacaaaga
      361 cctgggcaaa gaaaagccag aagctggcaa acaacgtgat gcaaacgaag acgtaagcaa
      421 caggtgagcc gaagaggtct ttgagttctt ttttggccag tgtccaaatg gcgttcattg
      481 tgtgagctcc tgaaaaacag attccagcga ggactggttt tttgtgaggg tgttcagttg
      541 ccagttgttc gcgacggcca actgaaaaaa atcttcactg tgatcatgcg cgtcggaaca
      601 aacgacagcc acgtgatgtt tgtcgtcttg tgaactcgtg accgtcaacg aaagacagaa
      661 cggacaggca cggatgatat tttcgatgtc ggtcagatta ccgcgcaggc agatgtggta
      721 ttggttttgt tcctgggttt tctgacgcaa ttcatccgct gtgccttggg ccaccagtga
      781 gccgttgtgg atcacgagca gacgatcaca ggtttgtgtc agttccgaca agatgtgtga
      841 agacaacaag acagtttttg ttttgccaat atttttcagg agggttcgga tctgcacgac
      901 ctgattgggg tctaaaccaa tcgtaggctc gtcgaggatc aaaacgggcg ggtcgtgcaa
      961 gaaagcctgt gcgaggccaa cccgttgacg aaaccctttt gacaaggtgc ccattttgcg
     1021 gtccaagatc gaagcaatgt cgcattgttc gatgacgcag gacaactgtt gcttaagttt
     1081 ctttcggtcc aagccacgca ttccccccgc gaaacgcaag aaatcaattg tgaacatgtc
     1141 gtcatagagg gcagacgatt cgggaagata gcctacctgt gccttggttt tgagggggtt
     1201 ttgttgtaca cacaagccat tgatggtgac ctcccccgag gagggagcga gataaccggt
     1261 gatgaccctc atcgttgtgg acttgccagc accattgggt cccacgaaac ccacaacctc
     1321 gccggtagcg atgtcaaaac tgaggttgtt gacagcacga acgttcccga aggacttcgt
     1381 catattttta agagagatca tttgtctgta aagcgcagat acatcttgat cgttttgttg
     1441 gagggaaaca gtttttcggc cgccttccaa accttcttgg cctcatcttt tttgcccagg
     1501 gagtaatacg taatacccaa ttggatgtga atgtcggcaa aggccttgtt ggtcttgatg
     1561 gcctttttga gttcttccaa cgcgcctttg gtattgccat tttcacgcag acacacagcc
     1621 atcttggtgc ggatatcggc ataactttcg agctccaagg cttttttgta ctcaaggatg
     1681 gcctcgttgt aaagccccac acccttgtag cgatcggcta tttcggcgtg tttgttggcc
     1741 agcttggacc tgatgaatgg atccatctcg gctttcttgg ccttggcatc cttgcgggag
     1801 cggttgacca gtttttttgc gttttcgtat tcgcccagat cgttgtgcaa gatgcttaaa
     1861 ttcagcatgg cctccgtata gcggggattg atttgcaggg cctttttaaa gtttttgata
     1921 gcttcaccat aacgtccttc ttcgtgggaa atgattccca ggcaattgta gacatcggcg
     1981 tactcttttt tttgggccac aacttttttg aagagttctt ccgctttttt gtatttttgt
     2041 gtcttgaaaa aatattttcc ttctt
//
LOCUS       DATDUN010000672         1104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_112978, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000672 DATDUN010000000
VERSION     DATDUN010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112978"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VJC18_09390"
     CDS             complement(<1..520)
                     /locus_tag="VJC18_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45637.1"
                     /translation="MFRCLTSLLGLTTKISLETMATIAVANAPCCAEPVDDDSSKPPE
                     NVGTRAVPTWATKSVPGHPYFTATGASPREGSARLNQHAPATAAEMISSQTQPLEKEI
                     DFLSQLDPQVRAFMRWLSLHPSLQTTGYYAPTWNHLDPEQRLMVLGCALMAGFSIRKS
                     GVVKGVSDCLYCQ"
     gene            complement(607..>1104)
                     /locus_tag="VJC18_09395"
     CDS             complement(607..>1104)
                     /locus_tag="VJC18_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45638.1"
                     /translation="APGLDELVAALIRQTLRTPTDREQFGGMDELVSPFIESSERFVW
                     NQDQVIHDPEANQGPLGVVEYAWFAILKEREFAMKYAPREGFTRDFVAALQRHPQHEI
                     THADFVRIIAETSGENLDTLLAGNDFLNTLYHDPAPLTCRQQFGRPFQRTGKFLRAAW
                     QLLTN"
BASE COUNT          264 a          272 c          299 g          269 t
ORIGIN      
        1 tctggcagta aaggcaatcg ctcacgcctt ttacaacacc acttttgcgg atggaaaatc
       61 ccgccatcag ggcacaaccc agaaccatca gcctttgctc tggatccaga tgattccacg
      121 tgggtgcata atagccggtg gtctgcagac tcgggtgcaa agacaaccag cgcatgaagg
      181 cccttacctg gggatcgagt tggctaagga aatcaatttc tttttccaag ggttgagttt
      241 gtgagctgat catttctgcc gcggtggcgg gggcgtgttg attgagccgt gcggacccct
      301 cccgtggtga tgctcctgtg gcagtgaaat aggggtgtcc gggtacggat ttggttgccc
      361 aagtggggac ggcgcgtgtg ccaacatttt cgggaggctt gctggagtcg tcatcgactg
      421 gctcagcaca gcagggggca ttggcaaccg caatcgtggc cattgtctct agggagattt
      481 ttgtggtgag ccccagtaat gatgtcagac atctgaacat aggtgatttt tgacagacaa
      541 taaaaaagca aatctagata aaatgaccaa acttgtctct tgtcaatctt tgaccccgca
      601 cattttctaa tttgtcagaa gttgccaagc cgcgcgcaga aattttcctg ttctttggaa
      661 aggcctccca aattgttgtc tgcacgttaa gggagccgga tcgtggtaca gagtattgag
      721 aaaatcattt ccagccagca aggtgtcaag attttcgcct gaggtttcgg caatgatacg
      781 aacaaaatcg gcatgagtga tttcgtgttg gggatgtcgt tgcagggccg ccacaaaatc
      841 cctcgtgaac ccttctctgg gcgcgtactt catggcaaac tcgcgctcct tcaaaatggc
      901 aaaccacgcg tactccacga cccccaaggg gccttggtta gcttcgggat catgaatcac
      961 ctgatcctga ttccagacga agcgttcaga agattcaata aagggactga cgagttcatc
     1021 catgccccca aactgttcgc gatcggtggg ggttcgcaag gtttgacgaa tcagggccgc
     1081 caccaattca tccaagccag gagc
//
LOCUS       DATDUN010000673         2419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_367335, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000673 DATDUN010000000
VERSION     DATDUN010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367335"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /gene="pilM"
                     /locus_tag="VJC18_09400"
     CDS             <1..282
                     /gene="pilM"
                     /locus_tag="VJC18_09400"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HLD45639.1"
                     /translation="KNTIHHFFSRLAIEIQRSLDGYMLALERRKVDRIFLCGGGSQHQ
                     GLTNYLTSTLAIPVSLFNPFEKIDTTQFPREQFIKKQALFAVCCGLAID"
     gene            289..825
                     /locus_tag="VJC18_09405"
     CDS             289..825
                     /locus_tag="VJC18_09405"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HLD45640.1"
                     /translation="MHINFATKKKLSLHLPPLNQLELNRMVLLIIAGVVILFMATFGL
                     VQKIRLDKFIRERTRLENTVTKNPELPEVVPENTTGLSKSSIIRLFETRTRWSAVLSD
                     LSKTTPKGIWLTQIKNVANNQRDLELTGQASDQNVIAGFLEKLKKQNECQNVKLQSSA
                     QTQNNTKPMIVFNILCSL"
     gene            822..1388
                     /locus_tag="VJC18_09410"
     CDS             822..1388
                     /locus_tag="VJC18_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45641.1"
                     /translation="MKVVLEKVSVFQKPQRREVIVFGITLLGFLFVFFKSCWFPSTDA
                     IALVQKETQELLERQNQIAKTNQDTKRKTSEDLPTNTAAAYTLWANQFVKSPDVLLMK
                     EFSNPAIIKDLRIQNIEFADSKQDGDLTIQGFKLAVEGSFVSLRSYLNKLENLPIIFI
                     TRNITINVTNEDLGRINAELQGEAYGWE"
     gene            1375..1758
                     /locus_tag="VJC18_09415"
     CDS             1375..1758
                     /locus_tag="VJC18_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45642.1"
                     /translation="MVGSKRLFCLIGLVFLISLSVPTLAGTQHQTLSGLNLKDFDSQT
                     KNEVAWQNNPFVKPASDVAVGELSLTGIIYSQTRSAAIINSQIVEIGDKIGSHEVVSI
                     EPSGQVVLRTESGLFRLKMKGAITP"
     gene            1755..>2419
                     /locus_tag="VJC18_09420"
     CDS             1755..>2419
                     /locus_tag="VJC18_09420"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HLD45643.1"
                     /translation="MILSHKTKTSTAFLFFVFLSLTLVLTFSALGEEGTYRVEFNNAD
                     ITDAVRLLAKVGNKNIVVSDKVAGKVTASFDKITVGDALSAVLNSNGFGIVDRNHVLQ
                     VLGKEEMTAFGEDLTTSSYNLQYAKAEDILPHISSLVSERGSVIADKRTNTMHVRDTQ
                     ASIQNISTLVFRLDRKGRQVLIEAKIIEATENFVRGLGIQWGVTRSGGNIQTAGLTTV
                     GTD"
BASE COUNT          655 a          608 c          571 g          585 t
ORIGIN      
        1 aaaaacacga tccaccattt tttttcgcgc ctggccattg agattcagcg atcgctggat
       61 ggttacatgc tggccctgga gcgacgaaag gttgatcgca tctttctttg cggcggcggg
      121 tctcagcatc aaggtttgac aaattatctg acctcgacgt tagccattcc tgtttctctg
      181 tttaaccctt ttgaaaagat tgatacaacc cagtttccca gggaacaatt tatcaaaaag
      241 caggccttgt tcgccgtgtg ttgtgggctg gctattgact gaagacttat gcacattaac
      301 tttgcgacaa agaaaaaact atcgctccat ctgccccccc tcaaccagct tgagttgaac
      361 cggatggtcc tgctcatcat tgccggagtc gtgattcttt tcatggcaac ctttggtctg
      421 gtgcaaaaga tcaggctcga taaattcatt cgggaacgaa cacggctcga aaacactgtg
      481 accaaaaacc ctgaactgcc agaagtcgtc cccgaaaaca caactggctt gtcaaaaagc
      541 agcatcatca ggctttttga aaccagaacc cgatggtccg cggtgctaag cgatctgtca
      601 aaaaccacgc cgaaagggat ctggctcacg cagatcaaga acgtcgcaaa caatcagagg
      661 gaccttgagc ttaccggaca ggcctctgac caaaatgtga tcgcgggttt tctggaaaaa
      721 ctcaagaaac aaaacgagtg tcaaaatgtg aaactgcaat catcggcaca aacgcaaaac
      781 aacacaaagc ccatgattgt ttttaatatt ttgtgttcct tatgaaagtt gtcctggaaa
      841 aagtcagtgt gtttcaaaaa ccacagagac gcgaagtgat cgtgtttggc atcacgctat
      901 tgggttttct gttcgtgttt ttcaaatcgt gctggtttcc cagcacggac gctatcgcgc
      961 tggtccaaaa agaaacccag gaattgctgg agagacaaaa tcagatagcc aagaccaatc
     1021 aagacacaaa aagaaaaacc tctgaagacc tgcctaccaa tactgccgcg gcgtacactc
     1081 tctgggcgaa ccaatttgtc aaatcacccg atgtgttgtt gatgaaagaa ttttcgaatc
     1141 ccgccatcat caaggacctg cgcattcaaa acatcgagtt tgcggacagc aaacaagatg
     1201 gtgacctgac cattcagggg tttaaacttg ccgttgaggg gtcttttgtt tcactacgct
     1261 cttatctgaa caaattagaa aacttgccaa tcatcttcat aacccgtaat atcaccatca
     1321 atgtgaccaa cgaggatctc ggccgcatca acgccgagct tcagggagag gcttatggtt
     1381 gggagtaaaa gactattttg tttgattggt cttgttttct tgatatccct gtccgtgcca
     1441 acgctggccg gaacacagca ccaaacgctt tctggcctta atctgaagga ttttgattca
     1501 cagaccaaaa atgaagtcgc ctggcaaaac aaccctttcg tgaagccggc cagcgatgtg
     1561 gctgtggggg aactgtccct gactgggatc atttattccc aaacccgttc agccgcgatc
     1621 atcaacagtc agatcgtgga aattggcgat aagattggtt cacatgaggt tgtttccatt
     1681 gaaccaagcg gtcaagtcgt gttacgaaca gaaagcgggc tttttcgact aaagatgaag
     1741 ggggctatta ctccatgatc ctttcgcaca aaacaaaaac cagcaccgcg ttcctgttct
     1801 ttgtgtttct ttccctaacg cttgtgctga ctttctccgc gctgggtgaa gagggaacct
     1861 accgcgttga gttcaacaac gccgacatca cggatgccgt caggttgctg gccaaggtgg
     1921 gcaacaagaa catcgtcgtt tccgacaagg tggccggcaa ggtcaccgct tctttcgaca
     1981 aaatcacggt tggcgatgcg ctaagcgctg tgttgaactc gaacggattc ggcattgtgg
     2041 atcgcaacca cgttttgcag gttttgggaa aagaggagat gacggccttc ggcgaagacc
     2101 tcaccacgtc atcatataat ttgcaatacg ccaaggccga ggacattttg cctcacatct
     2161 cttctctggt ctcagagaga ggctctgtca ttgccgacaa acgcacaaac accatgcatg
     2221 tgcgggatac acaggccagc attcagaaca tctcgactct cgttttcaga ctcgaccgca
     2281 aggggagaca ggtcctcatc gaagccaaga tcatcgaggc cacagaaaat ttcgtgcgcg
     2341 ggcttggtat tcagtggggc gtgacccgct ctggcggcaa cattcaaact gccggcctca
     2401 ccaccgtggg aaccgacga
//
LOCUS       DATDUN010000674         1756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_286273, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000674 DATDUN010000000
VERSION     DATDUN010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286273"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..553
                     /locus_tag="VJC18_09425"
     CDS             164..553
                     /locus_tag="VJC18_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45644.1"
                     /translation="MSRIGNQYPTCHVESVPSRELATVRSADQGVQKLSGVSALEPIE
                     VVDAELVDDGADVGLRESQGLYGDSFVRYETTAQAQYQYLTAYDSESYFKNELQKKGY
                     GENPYSDDRLGKYKSQIMRGKSKDVFV"
     gene            582..1352
                     /locus_tag="VJC18_09430"
     CDS             582..1352
                     /locus_tag="VJC18_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45645.1"
                     /translation="MFQNAMSKQFKNIYILGGHELAYSYYQKILQAKKEGRIACENLL
                     FVTPDPQCHAAVALKKENLIPQNFLIQKKYTDFILDCVGCDQNWHNENRVIPDHTAGH
                     VLLRVLMDLAPGYSVRLSPIELAIGTPFLKKFEQDAIWAMSFATWTCPMYCDEPALCP
                     HTKEPRTGDLNEMCFAKENSFSQVRAGVFERANAVLYSFACQPLIDEIVSFDFAPLTG
                     QINDYRHRIATGVLRKVYVFTHSHCHGILGCFVCGLGR"
     gene            1475..>1756
                     /locus_tag="VJC18_09435"
     CDS             1475..>1756
                     /locus_tag="VJC18_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45646.1"
                     /translation="MRKKNQLRTVRFSEEEAAQLEVYLAQNPVFESFSSLARVATLTL
                     IQQKCAVQLNPVIISSDIKRPPFIWDYELDEVQVREILSRSNTDPKKIWL"
BASE COUNT          482 a          388 c          412 g          474 t
ORIGIN      
        1 acacctcaga atctagccac tttgagaatg tagtccccaa attctcattc tgatggggta
       61 tgagggttgc gacaaaccga tatcctgtcg atatcattcg taaaaaccga tctgattttg
      121 actcttgctt ggtatggatg ttgcataata cactcatgtg agagtgagca gaataggcaa
      181 tcagtacccc acatgtcatg tcgaaagcgt cccgtcgcgt gagcttgcga ctgttcgttc
      241 tgccgatcaa ggtgttcaga aattaagtgg cgtttcagcg ctggagccca tcgaggttgt
      301 ggatgccgag ctggttgatg acggtgcgga cgttggtttg agagaatcac aggggcttta
      361 tggtgattct tttgttcgtt atgaaactac cgcacaggcg cagtatcagt acctcacagc
      421 ctacgattcc gaatcctatt ttaaaaacga attgcagaaa aaggggtatg gggagaatcc
      481 ttattcagac gatcgtctgg gcaaatacaa atcacaaatc atgcgcggca aatccaaaga
      541 tgtctttgtt tgattaatca acttgactcc acacaaacac catgtttcaa aacgccatgt
      601 ccaagcaatt caaaaacatt tatatcctcg gtgggcatga gctcgcgtac tcctattatc
      661 aaaaaattct gcaggccaaa aaagaagggc gcatcgcgtg cgagaacctc ctgtttgtga
      721 caccggatcc gcaatgccac gcggcagttg ctctgaagaa agaaaatttg attccgcaaa
      781 attttctgat tcaaaaaaaa tataccgatt tcattctgga ctgtgtgggc tgtgatcaga
      841 attggcacaa cgaaaatcgg gtcattcccg atcacacggc ggggcacgtg ctgttgcgtg
      901 tattgatgga tctggcccct ggttattctg tgaggctctc gcccattgag ctggcaatcg
      961 gcacaccttt tttgaagaaa tttgagcagg acgcgatctg ggcaatgagc tttgctactt
     1021 ggacgtgccc gatgtactgt gatgaaccgg ccctgtgccc gcacaccaaa gaaccacgaa
     1081 caggggattt gaatgaaatg tgttttgcca aggaaaattc tttttcccaa gtcagggctg
     1141 gtgttttcga aagagcaaac gcagtactat actcatttgc gtgtcaacct ctgatcgacg
     1201 aaattgtcag ttttgatttt gctcccctga ctgggcaaat caatgattac agacaccgga
     1261 tagcaacagg cgtcctcagg aaagtgtacg tattcacaca ctcacattgt cacgggattt
     1321 tgggttgttt tgtttgtggt ttgggcagat gacctttttc gattgattgg tgttttgggc
     1381 tgatatcatt aatgaattta tctcaacaaa agacagaagc ccccttgctc acaataatat
     1441 tgatatatgt attcgaatcg aatacaataa cgatgtgagg aaaaaaaacc aactcagaac
     1501 agtacgattt tcggaagaag aagcggccca actcgaagtg tatcttgcac aaaatcccgt
     1561 ttttgagagt ttttcttcac tggcgcgagt ggcaacgctc accttgattc agcaaaaatg
     1621 cgcggttcag ctcaaccctg ttatcatcag ttcggacatc aagcgccccc cgttcatttg
     1681 ggactacgag ctggacgagg ttcaggttcg cgaaattttg agtcgatcaa atacagatcc
     1741 caaaaaaatt tggctc
//
LOCUS       DATDUN010000675         9285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_155542, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000675 DATDUN010000000
VERSION     DATDUN010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155542"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VJC18_09440"
     CDS             <1..369
                     /locus_tag="VJC18_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45647.1"
                     /translation="IKKAGELFPDRPEIKQIIEQAWAEVKVLGKQAISADMAYEIPEG
                     VEGREIIDIFRLDPRLITELENKVQATTLPEGVRRCKVGFVLDENLNASLVCIAYVSE
                     GGKLDLDNLIEGVPVVKFKV"
     gene            complement(437..1720)
                     /locus_tag="VJC18_09445"
     CDS             complement(437..1720)
                     /locus_tag="VJC18_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HLD45648.1"
                     /translation="MSLFAQNTAHLRPLADQLRPTTLDDFVGQKHILGPDKMLTRFIE
                     SGTVPSLIFWGPPGVGKTTLAKIIATYTKHHFITMSAVTSGIKEVKEVIEKAREQLEH
                     FSKKTILFIDEIHRFNKAQQDAFLPHVESGTITLIGATTENPSFEVISALLSRCQVVV
                     LEQLGHEELKAILNRALSKKEKSLLLDDPAKNTIIENSFGDARYLINILEDLMSLFPS
                     EQNLNAETVSEKIFKRASLYDKKSEHHYNVISAFIKSLRGSDPDAALYYLARMLEAGE
                     DPLFICRRMVIFASEDIGNANPGAVGVAIACKDSFDFVGMPEGWIPLAHCASYLAGSP
                     KSNASYAGYKKALGDVKKYGPLEVPLHLRNAATDLMKNLGYSDGYKYTHDYEGNKVEQ
                     QHLPDKIKDHKYYFPTENGFEIKIRDWLKKRAVSK"
     gene            complement(1974..2061)
                     /locus_tag="VJC18_09450"
     tRNA            complement(1974..2061)
                     /locus_tag="VJC18_09450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2025..2027),aa:Ser,seq:gga)
     gene            2348..4858
                     /locus_tag="VJC18_09455"
     CDS             2348..4858
                     /locus_tag="VJC18_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45649.1"
                     /translation="MKIKPFFSPDLWHTPPTGVASPLTAGDIIEVPPRLVSRIPQAGI
                     PVNPQARGSYPILNSLSSAHALRSRALAHTSPSVDAVPAFLASRLTAGSRVISSSSLH
                     AGAGLSSGITSPFATKPLEDGNHGASAVTERVDVAGSASELVLAEIRTCLQQKGVGTL
                     LLEKTQTEICQTTDSVIALSVEDKAFAKTSKPHDCSFTLNVTYCSSGPLLSISSLCVE
                     DYICNREDTATPVRLTVLKNGLIRIEGSNDYYLPVVCEEDGQVRAAVVVIRTTNPVVW
                     NADEIEDTFSFEQEAAEQKPKTVGSPKSGSQTGSKAEGSIVPEPVTSKRAIQYFSAPA
                     PFSSVAEANDFMARASRVRHNRETPLPIKQEIARLLGRIGCRGSFPKGQEGLRAMSRR
                     HASLVDLVNVMNLYDWLLARQLTYDDAVLFFLRLWREDRDSLRHPELMLQFWQSAIPS
                     LHTFSSFLTRDAVALVRYTQTCELAEGHKPLPFDTRLAQAFAQFFLDHRDFVPQRVQQ
                     VVAALTKEFNDPQTDFTIIFTAQHLHALYLETDQTPSTQTRGSNIPTDVDSEGQARPA
                     DATLEAALKSALLLAGVEDPQPFLRLLETLLDPQIDQAAWHQLMTAGLTDMALITRVL
                     ANAQQSPDYLERYLAVRAKLGRDDVVDSADLDSVDEADEDVFTGSQRKDVTHWEVYRA
                     LRQSGVSDEAERSLLVTFFELGAQDVKGELWSARFRLALATYNKHKDHFELFCVAVDL
                     FGLAIYEPYLELMLAYSPRVNDDEAILILAMALKKAMDEDEQALATQIRIIATNFARL
                     LGRQADFVTAYWQAYAKGTNPANFTRLMLSGIAAAFEE"
     gene            complement(4812..6119)
                     /gene="lysA"
                     /locus_tag="VJC18_09460"
     CDS             complement(4812..6119)
                     /gene="lysA"
                     /locus_tag="VJC18_09460"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HLD45650.1"
                     /translation="MTQFAHFTYHDNRLFCEDCDLERIVKKTGTPVYIYSYRALCEQF
                     QSFASAFESHKHLVCYSMKANSNQAILNTLFNLNCGADVVSGGEIKRALLAGCDPQKI
                     VYSGVGKTEQEIAYALDQNILMFNIESQQELLLIQSIAKTKNKVASISVRVNPNVDAK
                     THAYISTGMKKNKFGVNHQAALQIYQQALVCDHINPVGISCHIGSQITQTQPFADAMI
                     ILTKVLHDVKNMSISINYIDVGGGLGIRYRDEIIPSAQEYANSILGPLKDFGATLITE
                     PGRFLVGNAGILVTRVLYQKQGETSDLFTIVDSAFNDLKRPMLYGAYHEIVPVIKSAT
                     DALIPTNIEGPICETGDRFAKQRPLPPLKQNDLLAIMSTGAYGMSMASTYNSRPRAPE
                     VLVKDDNFFIIRERETIEDMLASEKVPDFILQMPPRCPKASTV"
     gene            7274..8521
                     /gene="rho"
                     /locus_tag="VJC18_09465"
     CDS             7274..8521
                     /gene="rho"
                     /locus_tag="VJC18_09465"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HLD45651.1"
                     /translation="MYLKELKEVDINELIAVANHMGVDNAAGLKRQEIIFAILQTQID
                     STGEIYGEGVLETLPDGFGFLRSEDYNYLPGPDDIYVSPSQIRRFNLRTGDTVFGQTR
                     PPKEGERYFALLKVDAVNFEPAELARHKVLFDNLTPLYPEQKFNLEHDKKETTSRIID
                     LLSPIGKGQRALVVAPPRTGKTMMLQKIANAITANHPEVALIVLLIDERPEEVTDMER
                     SVKGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVP
                     PSGKILSGGVDSNALHKPKRFFGAARNIEHGGSLTIIATALIDTGSRMDEVIFEEFKG
                     TGNSEIHLDRKLMEKRIFPCMDVNKSGTRKEELLLEPDVLSRVWILRKLLHSLNVIDT
                     MEFLHDKMKDTVNNADFLESMNR"
     gene            8719..>9285
                     /locus_tag="VJC18_09470"
     CDS             8719..>9285
                     /locus_tag="VJC18_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLD45652.1"
                     /translation="MRNKFSIHIFALIMFLVFSSCDTYFVATQNPSSQGATPDITGTP
                     SSFSQGIYETSDLGELCFQADCQPDYDELSAEFREDYAPEMAGNCSFDDDPNRTSQFL
                     LHHQAQGINGLDLLLSKEDLFFMAWVSLRYQINPHFLLGVISQESYGDCAAVSYAGAE
                     GCFQITNDYGRAQLEDSYPDRVESWYWNE"
BASE COUNT         2303 a         2166 c         2446 g         2370 t
ORIGIN      
        1 atcaaaaaag cgggagagct gtttcccgac agaccggaga tcaaacaaat cattgagcag
       61 gcatgggctg aggttaaagt gctgggaaag caggccattt ctgcagatat ggcgtacgaa
      121 attccggagg gcgtcgaggg gcgggagatc atcgatattt tccggctcga tcccagactg
      181 attactgaac ttgaaaacaa ggttcaggcc acgaccctgc cggagggcgt gcggcgttgc
      241 aaggtagggt ttgtgctcga tgagaatctg aatgcgagcc tcgtgtgcat tgcctatgtg
      301 agcgaggggg gcaaattgga tctggataat ttgatcgaag gcgtgccggt tgtgaaattt
      361 aaagtatagt aggggccggt ccctccagag gcgggcacag cgcgaccggc ctctacggct
      421 cgcgatgaca ttctgtttat ttcgatacag cccgtttttt taaccaatcc ctgattttta
      481 tttcaaaacc attctcggta ggaaaataat atttgtgatc cttgattttg tcgggcaggt
      541 gttgttgctc caccttgttt ccttcgtaat cgtgtgtgta cttgtaaccg tcactgtaac
      601 ccaaattctt catgaggtcg gtggcggcat tgcgcaggtg tagcgggact tcgagcggac
      661 cgtatttttt aacgtcaccc agggcttttt tgtagccagc ataactggcg ttgctttttg
      721 gagaacccgc cagatacgat gcgcagtggg ccagagggat ccagccttcc ggcatgccca
      781 caaaatcaaa cgagtccttg caggcaatgg ccactcccac cgcgccagga ttggcattgc
      841 cgatgtcttc ggaggcaaag atcaccatac gacggcagat gaacaaaggg tcctctcctg
      901 cctccagcat gcgggccaga taatacagcg cggcatcggg atcggagcca cgcaggctct
      961 tgatgaaagc agaaatcacg ttgtagtggt gttccgattt tttgtcgtac aaagaagcgc
     1021 gtttgaaaat tttttctgaa accgtttcgg cgttgagatt ttgctcgctc gggaacagtg
     1081 acataaggtc ttcgaggatg ttgatgaggt aacgtgcgtc accaaaggaa ttttcgatga
     1141 tggtattttt ggcagggtca tccagcaaca ggcttttttc ctttttagaa agggcacgat
     1201 tcaaaatggc cttgagttct tcgtggccaa gttgttcgag gaccaccacc tgacagcgcg
     1261 agagaagggc cgagatgacc tcgaagctcg gattttcggt tgtggcgcca atcagtgtga
     1321 tggtgcccga ctccacatgt ggcaaaaagg cgtcctgttg ggccttgttg aatcggtgaa
     1381 tctcgtcgat gaacaaaatg gtttttttgg aaaaatgttc aagctgttct ctggcctttt
     1441 cgatgacttc tttgacttcc ttgatgccgc tggtcacggc gctcatggtg atgaagtgat
     1501 gcttggtata agtggcgatg attttggcta acgtggtttt gcccaccccc ggcggccccc
     1561 agaaaatcag ggatggtaca gtgcccgatt cgatgaatcg tgtgagcatt ttatcgggac
     1621 ccagaatgtg tttttgtccc acaaagtcat cgagtgttgt gggtctgagt tgatcggcga
     1681 gcggtctcaa atgggctgtg ttttgggcaa agagggacat ggtgtttgtt cgcggtctgt
     1741 gtcccgacgc attgtgtggc tctaggtggc gacgtcgctc gaagctgtgc gaagcatagc
     1801 gcagtgcgga gagagcggga ttcggtcaca aataaatttt ctgacggaaa tttttcgcga
     1861 ccccgcctcg cggtgctcgc tttgcccttt gggcaaactc gcacaacgct gtggcattcg
     1921 aatcccgcac gcaggcaaag cagtttgcct gcttctctcg ctcacttcgt tcgcggagag
     1981 agcgggattc gaacccgcgg tacggtatta gcgtacacac gctttccaag cgtgcgcctt
     2041 aaacctctcg gccatctctc caataatgca aaatttcatg ccgagaggtt taaggcgcaa
     2101 aacgcgccta ctgtttgttt gccagcctcc ggctggacct tctcggccct gaagccccgc
     2161 tttgcggggc aaaactgttc cccgctttgc gggcaacgtg aagagcgcct atattaccgg
     2221 cacaaacctg agtcaaccca tgtctttgtt gacaacctct tgaaattcaa ggacatgcct
     2281 ttctgccgaa tgaaaaaaaa cacaaataat atagcaacat ttatttgcct tggtcgatac
     2341 agaccccgtg aagatcaaac catttttcag tccagactta tggcacacac cgccaacggg
     2401 cgtcgcgtca cccttgacag caggtgatat catcgaagtc cctcccagat tggtgtccag
     2461 aattcctcaa gcaggaatac ctgttaatcc ccaagctcgt ggaagttatc ccatattaaa
     2521 ttctttgtct tccgcacacg cgctgagatc acgcgctttg gcgcacactt ccccgtcggt
     2581 tgatgcagtg ccagcctttc ttgcgagtcg attgacggca gggtcacgcg tgatttcctc
     2641 atcgtctttg catgcaggtg cgggattgtc ttctggaatc acctcgcctt ttgcaacgaa
     2701 accacttgaa gatggcaatc acggcgcgtc cgcagttacc gaacgagttg atgttgccgg
     2761 atctgcgagc gaacttgtgt tggcagaaat caggacctgt ctgcaacaaa aaggagtggg
     2821 gactctcttg ctggaaaaaa cacagactga gatttgtcaa acaacagatt ctgtgatcgc
     2881 gctttctgtt gaggataagg cgtttgccaa gacatccaag ccgcatgatt gcagttttac
     2941 tcttaacgta acttattgtt catcaggtcc gttgttatca ataagcagtc tttgtgtaga
     3001 ggattatatt tgtaatcggg aagacacggc aacaccggtc cggcttacag tattaaaaaa
     3061 tggcctgatt cgcatcgagg gatcgaatga ttattattta cctgtggtct gtgaggaaga
     3121 cggtcaagtg agagcggcgg tagtggtcat cagaacaacc aatccagttg tgtggaatgc
     3181 agatgagatt gaggacacgt tcagttttga gcaggaggcg gcagaacaaa aacccaaaac
     3241 tgtaggatcg ccaaagtcgg gatcgcagac ggggtcgaaa gcagagggta gtatcgtccc
     3301 cgaaccagta acctcaaaga gggccattca gtatttttct gcccccgcac ctttttcaag
     3361 tgtcgcagag gccaatgatt ttatggcgag ggccagccgg gtcaggcata atagagagac
     3421 acctttgccg atcaagcagg agatcgcacg attgcttggc cggataggat gcagggggtc
     3481 atttcccaag ggacaggaag ggcttcgtgc gatgagccgg agacatgcat cccttgttga
     3541 tctggtcaat gtgatgaatc tgtatgactg gctgttggcg aggcaactca cttatgatga
     3601 tgccgtgctg ttttttcttc ggctatggcg agaggacagg gactctctgc gccatccgga
     3661 attgatgttg caattctggc agtctgccat tccctctttg cacacttttt cctctttttt
     3721 gacacgtgat gcggtcgcgt tggttcgcta tacccaaacc tgtgagttgg cagaagggca
     3781 taagcccctc ccatttgaca cacgtctcgc gcaggccttt gcccagtttt ttttggacca
     3841 tcgggatttt gttccgcaaa gggtacagca ggttgtagcg gcactcacga aagaattcaa
     3901 tgatccccaa accgatttta caataatttt tacagcacag catttgcatg ctttgtactt
     3961 ggagacggac cagacaccct caacacagac acgaggcagt aatataccga cagatgtgga
     4021 cagtgagggg caggccagac ccgcagacgc aaccttggaa gcggcattga agagtgccct
     4081 gctgttggct ggggtggaag atccgcaacc tttcttgcgg cttcttgaaa cgctgcttga
     4141 tcctcagata gaccaagctg cctggcacca attgatgaca gcgggtttga cggacatggc
     4201 gttgatcaca cgtgtgctgg ctaacgcaca gcagtcgccc gattatctgg agcggtattt
     4261 ggctgttcgt gctaaattgg ggcgggatga tgttgtcgat tcggccgacc ttgatagtgt
     4321 tgatgaggca gatgaggatg tttttactgg tagccagcgc aaggatgtca ctcattggga
     4381 ggtgtaccgg gcactgcgac agagtggcgt gagcgatgag gccgaaaggt ccctgcttgt
     4441 tacgtttttt gaattagggg cgcaggatgt taagggagaa ctttggtcag cacggtttcg
     4501 tttggccctt gccacgtata acaagcacaa ggatcacttt gagcttttct gtgtcgctgt
     4561 ggatctcttt ggtctggcaa tatatgagcc ctacctcgaa ttgatgttgg cctacagccc
     4621 aagggttaat gacgacgaag ccattttgat tctcgccatg gccctgaaaa aggccatgga
     4681 tgaagacgag caggcactgg cgactcagat cagaattatt gcgacgaatt ttgctcgttt
     4741 attgggtagg caggcggatt ttgtgacagc gtattggcag gcatacgcca aaggtaccaa
     4801 tccggcaaat tttacacggt tgatgctttc gggcatcgcg gcggcatttg aagaataaaa
     4861 tccggcacct tttcactggc aagcatgtct tcgatggtct ctctttccct gatgataaaa
     4921 aaattgtcat ccttgaccaa cacctctggt gcgcgaggcc gtgaattgta agtgctggcc
     4981 atgctcatgc cgtaggcccc ggtgctcatg atggccaaca gatcattttg tttgagggga
     5041 gggagggggc gctgtttggc aaaacggtca ccggtttcgc agatggggcc ttcgatgttg
     5101 gtggggatca gcgcgtccgt tgcggatttt atgacgggga caatctcatg gtaggccccg
     5161 tagagcatgg ggcgtttgag atcattgaag gcggaatcga caatggtgaa caaatcactc
     5221 gtttcgccct gtttttgata aagcacacgg gtcaccaata tcccggcgtt gcccaccaga
     5281 aaacgcccgg gttctgtgat gagggttgcc ccaaaatcct tgaggggacc caaaatactg
     5341 ttggcatatt cctgtgcgga gggaataatt tcatcgcggt accggatgcc cagaccgccg
     5401 ccgacatcaa tgtaattgat gctgatgctc atgtttttca catcgtggag gacctttgtc
     5461 aatatgatca tcgcatcggc aaatggttgt gtttgcgtga tttgcgagcc gatatgacag
     5521 gaaatgccta cgggatttat atgatcgcag acaagggctt gctgatagat ttgcagtgcg
     5581 gcttgatggt tgactccaaa tttgtttttt ttcatcccgg ttgaaatgta cgcatgagtt
     5641 ttggcgtcga cattgggatt gacgcgcact gagatggatg cgactttgtt ttttgttttg
     5701 gcaatgctct gaatgagcaa caactcttgc tgactttcga tgttgaacat gaggatgttt
     5761 tgatccagtg cgtaggcgat ttcttgttcg gtcttgccca caccggaata cacaatcttt
     5821 tgaggatcgc aaccagccaa cagggctcgt ttgatctcac cgccgctgac cacatcggcc
     5881 ccgcaattca gattgaacag ggtattcaaa atggcctgat tggaattggc cttcatggaa
     5941 taacaaacca gatgtttgtg tgattcaaaa gccgaggcga agctctggaa ttgttcgcaa
     6001 agtgcgcgat aactgtaaat gtaaacgggt gtgcctgttt ttttgacaat gcgctcaaga
     6061 tcgcaatcct cgcagaacaa acggttgtcg tgataggtga agtgggcaaa ctgtgtcatg
     6121 ggagggtgag taatcgctca tagtccaaag gtcaacccat cgacagagtt taccccgagc
     6181 gaagccgagg ggctcagggc aggcccatgg cttaggcaaa acagattgtt cattattctc
     6241 tgttttaccc taaactttga gccctatgcc cactcgtgaa cacctgaata cgtaaagggc
     6301 acctctaaaa acaatcttct actgcaaaaa catgctctgg cctgcaactt cgtcagattt
     6361 ctcaaaaagt cctcaacgtg gcgttcctta cacgtctgcg ggctttttgt tcatcttcct
     6421 cgtttcggct cagatcatgt tttttcgtta cgaacatagt ttttagaggt gccctaaaca
     6481 cttgaacaca tgggccaact caatacgccg tttgacatcc ctgcaaagag cgtatatact
     6541 aaggcagttc aaatcggggt ttaccccgtc agagaggcca atccacgata agcgtcgaaa
     6601 ttatcaaagc ttagcgtttg acaactatat agcagaagac atttgttgaa tttttgtcgt
     6661 gagatcataa ttgacccctg agttaggaaa gcatcaaata attcataatt catgagtatt
     6721 gaacattcct gtaaacaatc attctagcct gtgccacagg cgtgtatttg cctgctagca
     6781 gctagatcca tccacattag gaagtgatat ggtcacaaca aacacaactg aaatcagtca
     6841 agacacaggc gaggtcgtag aaaataaaac ggttgaaacg aaggtttccg aaacaggcgc
     6901 tgaagaacaa aaaccaaaac cagaaggcac cgacaaagat ctcgttgcag aacaggaccc
     6961 ccagcggaga agtccaagga ccaggcgtgc tgtcggttat accagcagtc aaggttcagg
     7021 acgtcgctat acgccgcgtt cttcacgttc cgtagacgac agagaccgag acagaggtcg
     7081 caacggttcg gatcgcaatg gcgattatcg gggatcagat tatcgcaata acgatcgcta
     7141 taacgaaaag cccattgatc gcaacctgga ccgattgcag gatgccccaa aaaacaaaca
     7201 accggttgtg attgatgaaa aagaactcga aaaagtggcc cccaatctgg tgcactactt
     7261 tacgggccaa aagatgtacc tcaaagagct caaagaagtt gatatcaacg agttgattgc
     7321 cgtggccaat cacatgggcg ttgataacgc ggcgggattg aaacgacagg aaatcatttt
     7381 tgccattttg caaactcaga ttgattccac gggagagata tacggagaag gtgttttgga
     7441 aaccttgccc gacggttttg gttttttgag atcagaagat tataattatc ttccgggtcc
     7501 cgacgacatc tacgtgtcac cctcgcagat tcgccgattc aatctgagga cgggggatac
     7561 ggtttttggt cagaccagac cgcccaagga gggagagcgg tattttgccc tgctcaaggt
     7621 cgacgcggtc aactttgagc cggccgaact ggctcgacat aaggtgttgt ttgataattt
     7681 gacccctttg tacccggaac aaaaattcaa tctggagcac gacaaaaaag aaacgacctc
     7741 gcggattatc gatctgttat cccccattgg caagggacag agggccttgg tggtggcacc
     7801 cccacgtact ggcaaaacga tgatgttgca aaaaatcgcc aacgcgatca cggccaacca
     7861 ccccgaagtc gctctgattg tcctgctcat cgatgagcga cccgaagaag tgaccgatat
     7921 ggaaaggtct gtcaaaggtg aggtcatcag ctcaacattt gacgaaccag cgacccgcca
     7981 cgttcaggtt gccgaaatgg ttatcgaaaa agccaaaaga ctggtagaac acaaaaaaga
     8041 tgtagtgatt ctgctggatt cgatcacgcg tctggcgcgt gcttacaata cagttgttcc
     8101 gccatcgggc aaaattttat ctggtggtgt cgattccaat gccttgcaca aacccaagcg
     8161 tttttttggg gccgcgcgaa acatcgaaca cggagggtca cttacgatca ttgctacggc
     8221 gctgatcgac acaggctcgc gtatggacga agtcattttc gaggagttca agggcaccgg
     8281 caacagcgaa attcatctcg atcgcaaact catggaaaaa cgcatctttc cctgtatgga
     8341 tgtcaacaag tccggtaccc gcaaggaaga gctgttgctg gaaccagatg tgctgagccg
     8401 tgtctggata ctgcgtaaac tcttgcactc actcaacgtg atcgacacca tggagtttct
     8461 gcacgacaag atgaaagaca cagtcaataa tgccgatttt ttggaatcga tgaacagata
     8521 aggccttaca gggctctatt tgcctgcata taacctctca aaatcacatc aatattagta
     8581 atccacagtc ttgtgttgtg gaattcgctt gctcatggtc cgaaacgtct gtaaatggcg
     8641 cagacgtttt tttgtcgtgc ctttccggaa acagtaaact ggaaatcatg atgaatcata
     8701 gaagaaccaa aggggatcgt gagaaacaaa ttcagtatcc atatttttgc cctcatcatg
     8761 tttttggttt tttcgagttg cgatacctat ttcgtagcaa cgcaaaatcc atcaagtcag
     8821 ggggctaccc ccgatatcac ggggacccca tcatcttttt cgcagggcat ttacgaaaca
     8881 tccgatctgg gtgagctgtg ttttcaggca gattgtcagc ccgattatga cgaactgtca
     8941 gctgagtttc gtgaggacta tgctccggaa atggcaggaa actgttcttt cgatgatgac
     9001 cccaaccgga catcgcagtt cttgttgcat catcaggcgc aggggatcaa cggactggat
     9061 cttttgttat ccaaagaaga tctgtttttc atggcctggg tttccctgag ataccagatc
     9121 aatcctcatt ttttactggg ggtcatttct caggaatcct acggtgattg cgcggcggtt
     9181 tcgtatgccg gggccgaggg ctgttttcag atcaccaatg actatggacg cgcacaattg
     9241 gaggacagtt accccgatcg tgtggaaagc tggtattgga atgag
//
LOCUS       DATDUN010000676         1785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_36612, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000676 DATDUN010000000
VERSION     DATDUN010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36612"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VJC18_09475"
     CDS             complement(<1..847)
                     /locus_tag="VJC18_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HLD45653.1"
                     /translation="MANELIMNVTPGETRIALLERGVLAELSIERETEMQVAGNIYFG
                     RINRVLPGMQAAFVDIGLEKAAFLYVADIVSDMFEGVFEDEVDEVGKNGDAGGDDEEG
                     GDKAPDDAEPTDSGSENGGSDQNANKDRNQGRGGRSSSVRKSRYRRAPLPRIEDVVKE
                     GQTIVVQVAKEPIRTKGARITSQISLPGRYLVYMPTVNHVGVSRRITSYDERIRLKEI
                     LRKNKPAQGGFIARTNSIGTCEEKIVEDMNNLKQIWQEIYKKQEKSRAPSLLYQDMDI
                     VLRAVR"
     gene            complement(1047..>1785)
                     /locus_tag="VJC18_09480"
     CDS             complement(1047..>1785)
                     /locus_tag="VJC18_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HLD45654.1"
                     /translation="KRSIPSGAQAIHDPRSTTHDQRSTMHRLRIRFAKKDWAIFYGHL
                     ELVNIIKRAILRCGLLVAYSEGFHPQMKLSLGPALSVGIESEFEYFDLALLQVIEPNE
                     FIEKMNLVLPSGVKLLSAEKVDLNAPSIYSAMQWVDFEIDLTSSPQSIDSQKIADGLE
                     SIKAGSEVAVERVSNKKEGLVRKISLNRYMNLKTAKVTDNKFLCFSIRSDDEGSLKPI
                     EVLEVLSRNSREELSGILIKKTGVVLN"
BASE COUNT          426 a          491 c          383 g          485 t
ORIGIN      
        1 cgcgcacggc ccggagcaca atgtccatgt cctgatacaa caaactgggg gccctggatt
       61 tttcctgttt tttgtaaatt tcctgccaaa tctgtttgag gttgttcatg tcctcaacaa
      121 tcttttcctc gcaggttcca atgctgttgg ttcgggcaat gaacccccct tgggccggtt
      181 tgtttttacg cagaatttcc ttgagcctga tccgttcgtc gtatgatgtg atccgacgag
      241 acacccccac atgattcacc gtgggcatgt acaccagata acgcccgggc agggaaatct
      301 gtgatgtgat cctggccccc ttggtccgga tcggttcctt ggcaacctga acgacgatcg
      361 tttggccttc cttgaccaca tcttcaatac ggggcaaggg ggcacgacgg taccggcttt
      421 ttctgacact gcttgaacgc ccgccccgac cttgattcct atccttgttg gcattttgat
      481 cactgccgcc attttcgctc ccggaatcgg ttggctcagc gtcatccgga gccttgtcgc
      541 caccctcttc gtcatccccg ccagcgtcac cattcttgcc aacctcatcc acctcatcct
      601 caaaaacgcc ttcaaacatg tcgctcacaa tatcggccac atataaaaag gcggcttttt
      661 cgagtccgat gtctacaaaa gcggcttgca tcccgggcaa tacccggttg atgcgcccga
      721 aatagatatt gccagcgacc tgcatttcgg tctcgcgctc gattgataat tccgccaaca
      781 cgccacgttc caacagcgcg atccgggtct cgccgggcgt gacgttcatg attaattcat
      841 tagccatttt ttatttcctt actggtgggg aatgacggga cggatctgaa gacactatgg
      901 tatagccgga tgactaacat caatcgaaac aaccccttcg ttgataaaca acaacgaaaa
      961 aggatttttc attttatgtc aaaaactttg gcaaaacctg tgtgttcatg agtgccgggc
     1021 caaaaggcct cgtcaagttc atccattcaa ttcagcacca caccagtttt cttaattaat
     1081 attcctgata actcttctct ggagttccgg ctcagcacct ccaaaacctc gatgggtttg
     1141 agactgccct cgtcatcgct ccgtattgaa aaacataaga acttattgtc agtcaccttc
     1201 gctgttttca aattcatata gcggttaaga ctgatcttcc tgaccaaacc ctctttttta
     1261 ttactcacgc gttccacggc aacctctgaa cccgctttga tactttcgag tccgtccgca
     1321 attttctgac tatctatgct ttgcggacta ctggtcaaat cgatttcaaa atcaacccac
     1381 tgcattgcag aatagatgga cggggcattt aagtcaacct tttcggcaga aagcaattta
     1441 acaccgcttg gcaaaaccaa gttcattttc tcaataaatt catttggttc aatgacttgc
     1501 aacaaggcaa gatcgaaata ttcaaactca ctttcaatcc ccacagacaa ggctggcccc
     1561 agggagagtt tcatctgcgg gtgaaatccc tcagagtaag ccaccaaaag gccgcaacgc
     1621 aaaatggccc ttttgatgat gttcaccaac tcaaggtgcc cgtaaaaaat cgcccagtct
     1681 tttttggcaa aacgaatacg gaggcggtgc atcgtggatc gttgatcgtg tgtcgtggat
     1741 cgtggatcgt ggatcgcttg tgccccagag ggtatggatc gcttg
//
LOCUS       DATDUN010000677         1081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_113087, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000677 DATDUN010000000
VERSION     DATDUN010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113087"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VJC18_09485"
     CDS             <1..660
                     /locus_tag="VJC18_09485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLD45655.1"
                     /translation="DLSVFPKDWHISLGPIRLALKPSANNQLGVFPEQCDSWQWITKK
                     IRNAGRPLRILNAFAYTGAATLMSSAAARDVEVCHVDGAKTSVTWAKQNALLSGLESK
                     SIRWIVDDVLKFLAREIKRGRTYDAFILDPPAFGRGAKTEWSLERDLPLLLDCVDQLL
                     SPHPCFVVLSCHAPHMIATDLAHQLEKLPAFAGKQAQTLNLTIASKQGHALPLGICAR
                     I"
     gene            875..>1081
                     /locus_tag="VJC18_09490"
     CDS             875..>1081
                     /locus_tag="VJC18_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HLD45656.1"
                     /translation="MKKIIECVPNFSEGRDQNIIRQITEQITSSDGVKLLDVDAGQAT
                     NRTVVTFVGSPDEVIEAALKAIKKA"
BASE COUNT          277 a          311 c          250 g          243 t
ORIGIN      
        1 gacctttcgg tgtttcccaa agactggcac atcagcctcg gtcccatcag actggcgctc
       61 aaaccatcgg ccaacaatca actgggtgtt tttcccgaac agtgcgacag ctggcaatgg
      121 atcacaaaaa aaatccgaaa tgcggggcgt cccctgcgca tcttgaacgc ctttgcctac
      181 acaggcgcgg ccacgctcat gtctagcgcc gcggccagag acgttgaggt ctgtcacgtg
      241 gatggtgcca aaacatcggt cacctgggct aaacaaaacg cactattgtc cggactggaa
      301 tcaaaatcca ttcgttggat tgtggatgat gtgctcaagt tccttgcacg cgaaatcaaa
      361 cgtggccgaa catacgatgc ctttatcctt gatcctcccg cctttggccg aggggcaaag
      421 accgaatgga gccttgagcg tgacctgccg cttttgctgg actgcgttga ccaattgctc
      481 agcccccatc cctgttttgt tgtcttgagc tgtcacgccc cgcatatgat tgccacagac
      541 cttgcccatc aactcgaaaa actcccggcc ttcgccggca aacaagccca aacattgaat
      601 ctcacgatcg cctccaaaca ggggcatgcc cttcctctgg gcatctgtgc caggatttga
      661 atatcgaata gcgaccaagg aatgtcgaat gtcgaaattt gtcatcccag cggacgctgg
      721 gatccagtta tatttgatat ggattccagc atgcgctgga atgacaacaa cttcataatt
      781 ccttgttcaa ctttcaggca tgcctgtcat gtcgcctaat cacacattgt ccaacacatt
      841 tgacgtccag aaacagagcc actatgtatg tcccatgaaa aaaatcatcg aatgtgtccc
      901 caatttcagc gaaggacgtg accaaaacat catcagacaa atcaccgagc agatcacctc
      961 cagcgacggt gtaaaattac tggatgttga cgcgggccag gccacaaacc gcaccgtggt
     1021 gacctttgtg ggatcccccg acgaggtcat tgaggccgcc cttaaggcca tcaaaaaagc
     1081 t
//
LOCUS       DATDUN010000678         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_199330, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000678 DATDUN010000000
VERSION     DATDUN010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199330"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1095
                     /locus_tag="VJC18_09495"
     CDS             280..1095
                     /locus_tag="VJC18_09495"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HLD45657.1"
                     /translation="MQEYLNLLKHILEKGERREDRTGTGTLSVFGYQMRFDLAQGFPL
                     VTTKKCHLRSIIHELLWFLKGDTNVKYLRDHNVTIWDEWADESGELGPIYGKQWTRWE
                     AGHGQTINQIQNAVDLITHHPDSRRILVSGWNVADLQVLISAKKSAPPPCHTLFHFYV
                     HQDRRLSCQLYQRSADSFLGVPFNIASYALLTMMMAQVTGCTPGHFVHTFGDAHLYLN
                     HLEQARLQLTREPRALPVMRLNPAVKSIFDFRFEDFTLTGYDPHPTIKAPIAV"
     gene            1296..>2037
                     /locus_tag="VJC18_09500"
     CDS             1296..>2037
                     /locus_tag="VJC18_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45658.1"
                     /translation="MSHALNVLNTQTHLALLTTALCRFPLSDRQQGRALTDEAHLLMT
                     APAWAGPDDDENLGVHVQALISQGEQIGMPPLSLETFRWTQTSKRAVIRQFASTLTAM
                     SDARLIREAKKKGQKTVPDAPVDELSRRYQEVKAQIGGLNPAGGVAFRGGSVPVDADA
                     GFTADLAEWTQANERLMLLRGALHLLVERFPARFSSEHAVEVYLDLAGEGTEVRIKNE
                     CFEEMLVCADDNSDVRVMPLFVEYLTENM"
BASE COUNT          487 a          493 c          571 g          486 t
ORIGIN      
        1 ggggtcaggg gttgtgtttt gattggacat attgactaac aatgggcaac acactaaacc
       61 taccagtgca agcatgtggt gtcattgcga ggacactggg cgaaccgagt gagcctaagt
      121 ggacgaagca atccaaagaa attgtcattc ctgcgaaggc aggaatccag aatgatagta
      181 agaatggatc ccagcctgcg ctgggatgac aattttgtgg atcgccacgc catgaaccgg
      241 ttccggttca cggctcgcga tgacggtctg ggcgctttta tgcaagaata tctcaattta
      301 ctcaaacaca ttctggaaaa aggcgagagg cgcgaggacc gcactggtac ggggacgctc
      361 agtgtgtttg gttaccagat gcgttttgat ctggcccaag gcttccctct ggtcaccacc
      421 aaaaaatgtc atttgcgctc catcatccac gaactgttgt ggtttctcaa gggcgatacc
      481 aatgtgaaat atctgcgcga ccacaacgtg accatctggg acgagtgggc cgatgagagc
      541 ggtgagttgg gtcccatcta tggcaagcag tggacacgct gggaggcggg ccatgggcag
      601 accatcaatc aaattcaaaa cgcggttgat cttatcacgc atcatccgga cagccgtcgc
      661 attctggtga gtggctggaa tgtggccgat ctgcaggtct tgatttctgc caaaaaatca
      721 gcgccaccgc cgtgtcacac gctgtttcat ttttatgtgc atcaggaccg cagactctct
      781 tgtcagttgt atcagcggag cgccgattcc tttttgggtg tgccgttcaa cattgcgagc
      841 tatgcgctcc tgacgatgat gatggcgcag gtcaccggtt gcacgccggg gcatttcgtg
      901 cacacgtttg gtgacgcgca tctttatctg aatcatctcg aacaagcacg tctccagctc
      961 acgcgggaac cacgggcctt gcccgtgatg agactcaatc cagctgtcaa atccattttt
     1021 gattttaggt tcgaggattt tacgctcact ggttacgacc cgcatcccac catcaaggca
     1081 ccgatcgccg tttgaatatc gaatatcgaa caaggaatcc ttcaactacg ttcaggacag
     1141 gcaaaaggaa tttcgaagta cagcacctcc atttcgacat tcgacgttcc ttgttcgtta
     1201 ttcgatattc acgtgtccac aactgtggac gaatatttca gcaccatctt gtttgttaag
     1261 cgtgttagga ataatagagg ccaagggaga atattatgtc acacgcactc aatgttttga
     1321 acacacaaac acatttggcc ctgttgacga ctgccttgtg cagatttccg ctgagcgatc
     1381 gtcagcaggg gcgcgcactc acggatgagg cgcatttatt gatgacggcg ccggcctggg
     1441 ccgggcctga tgacgatgag aacctgggtg tccatgtgca ggcgttgatt agtcaaggcg
     1501 agcagattgg catgccaccc ctctcgctcg agacctttcg gtggacgcag acatcaaaaa
     1561 gggcggtgat cagacagttt gccagcacgc tcacagccat gagcgacgcg cgattaatac
     1621 gcgaggcaaa aaagaaagga caaaaaactg ttcccgatgc ccctgtggat gaactctcaa
     1681 ggaggtatca ggaggtcaag gcgcagatcg ggggactgaa tccagcaggg ggtgttgctt
     1741 ttaggggagg gtctgttcct gttgatgccg atgctggttt caccgcagat ctggctgaat
     1801 ggacacaggc caatgagaga ctgatgcttc ttcgtggggc cctgcattta ctggttgagc
     1861 ggtttcccgc aaggttttca tcggagcatg ccgtcgaggt gtatcttgat ttggcgggcg
     1921 aagggactga ggtgaggatc aaaaacgagt gttttgaaga gatgctggtc tgtgcggatg
     1981 acaacagcga cgtgcgcgtc atgccactct ttgtggagta ccttaccgaa aacatgg
//
LOCUS       DATDUN010000679         3983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_76014, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000679 DATDUN010000000
VERSION     DATDUN010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76014"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VJC18_09505"
     CDS             <1..604
                     /locus_tag="VJC18_09505"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HLD45659.1"
                     /translation="LVAQGRYHFYEGHDLKIITLPIRMMKQLGIKHVILTNAAGTANP
                     SFHAGDFMLIDDHVNFTGHSPLRGIANEELGPLFVDQSNIYDRVLNDKLESLCQQKHA
                     NIPVCRGVYCCTMGPQYESAAEVRMFGKLGVDAVGMSTVPEAIVARQSGLKISGITCL
                     TNYGTGLSDQPLNHEEVSEMGKKRAPQFMVLLNELVSLLC"
     gene            complement(647..1258)
                     /locus_tag="VJC18_09510"
     CDS             complement(647..1258)
                     /locus_tag="VJC18_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD45660.1"
                     /translation="MSQDNRKIKDFLHEIRSKMSVLQALFDSPVTSESENPDFKQLFE
                     MSQTNYREIVNLMREMSNELYGTEPFGTGPCGQIQTAPQIIADDTDNILVIDDDVEIR
                     KQFSREMTKRGKNVVLLEKGEELLMRPLNLNDFATVVVDYQFENSALDGFDIVEYLKS
                     QKVRRIHLCTGCYDNPAVIKRAQELGIVSIISKPIPRHLWDVL"
     gene            complement(1255..2643)
                     /locus_tag="VJC18_09515"
     CDS             complement(1255..2643)
                     /locus_tag="VJC18_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLD45661.1"
                     /translation="MLLFNILCIDDDPSFLLATKSVLKKKYMVTTALSFEEGLKILET
                     QLIDLLFLDVKLGKENGIDLLYKIKELHPNLDVVMLSGERDPQLIVNAIRDGASDYIC
                     KPCPSEDLLAVVEKQLKNREIKDRYEALIEDMNECQSLKGFIGKSKSLTDVLDSAKRL
                     KGYNASVLIEGESGTGKELLARHLHGLEEDVRRPFIAVNCAAIPDNLLESELFGYEQG
                     AFTGASKKKVGKFELANGGDIFLDEINSLRPELQAKLLRALQEKEFYRLGGTQSIQVK
                     FRVIAATNVDLQEEVRNNHFRQDLLYRLRVVSLTMPPLRKRQEDIDELVAFFIKKHSR
                     ADQKKLVSPAVLEFFKQYKWPGNIRELENVLQSLIIMAVGDKIVLADLPQWMTATEQK
                     SVLDPNSYEAVPELKFSKIDQSLKNVMQKMEQEYIKKVIENTGGNVTEAARLLGVSRS
                     KVYYTLRDEETL"
     gene            3019..>3983
                     /locus_tag="VJC18_09520"
     CDS             3019..>3983
                     /locus_tag="VJC18_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45662.1"
                     /translation="MLVNSNVRIIYNREDPQHNDFNINLLRDLKVLTQPLSAADQAAF
                     LSDITEFLQSSPSRDNLVFESADKLAEALKQHVSRFGGLYVPKYFQQVVTQARRLEEH
                     VFEIRAVEGGLFYNFYPIGHQPGHLADNASARSTDVFVCPERSRTIYTTTEFDFSDIP
                     YDFFFPPNAVLKFNGNDVVSIMSSLPQIDPKERLSVLQRMMSLATTSSVALQSDALQS
                     DHKQNFLAYAALLKSLSGHFTLTKDLAISTLERVENMYQRCRHEMRHPAFYETTGITQ
                     AETLLKQHPEFGKVAYIEGIAQHIAQAGPAFELLGAITPAGLENP"
BASE COUNT         1025 a         1050 c          829 g         1079 t
ORIGIN      
        1 cctggtcgcg caggggcgtt atcatttcta tgaaggacac gatctcaaaa tcatcacctt
       61 gcccatccgc atgatgaaac aactgggaat caaacacgtg atcctcacca acgccgcggg
      121 gactgccaac ccgtcattcc acgcgggtga tttcatgctc atcgatgatc acgtcaattt
      181 tacgggacat tctccactca ggggcatcgc gaacgaagaa ctcggtccct tgtttgtgga
      241 ccagagcaat atttacgaca gagtcctcaa cgacaaactc gagagcctct gccaacagaa
      301 acacgccaac atccccgttt gccgcggcgt ttattgctgc acgatgggac cacaatacga
      361 atcggcggct gaagtgcgca tgtttggcaa attgggtgtt gatgcggttg ggatgtcgac
      421 agtgcccgag gccatcgtgg cccgccagtc gggcttgaag atcagcggca tcacttgttt
      481 gacaaactac ggcacaggcc tatcggacca gcccttaaac cacgaagaag tttccgagat
      541 gggcaaaaag cgagccccac aattcatggt tttgctgaat gagttggtga gtttactttg
      601 ctgaatacgt ttaagacacg ataaagaacg acacatttgt cgttctttac aacacatccc
      661 acaggtgccg cggaatgggt tttgatatga tcgacacaat gcccagctcc tgtgctctct
      721 tgatcaccgc aggattatca taacaccctg tgcacaggtg tatgcgtctg accttttgcg
      781 atttgaggta ttccacaata tcaaaaccat ccaaagcgct gttctcgaac tgataatcca
      841 ccacaaccgt ggcaaagtcg ttcaggttga gcggccgcat cagcaattct tcacctttct
      901 ccaaaaggac aacatttttc ccccgcttgg tcatttctcg ggaaaattgt ttgcggattt
      961 cgacgtcgtc atcgatcacc aagatattgt cggtgtcatc agcgataatt tggggcgctg
     1021 tctgtatttg cccgcaagga ccggtcccga agggttctgt cccgtacaat tcattggaca
     1081 tctccctcat caaattgaca atttcgcgat aattcgtctg gctcatttca aacaattgtt
     1141 tgaaatcagg attttctgat tcactggtca caggggaatc aaacaaggcc tgcagcaccg
     1201 acatctttga cctgatctcg tgaagaaaat cttttatttt tcggttgtcc tgactcatag
     1261 tgtctcctca tcccgcaacg tgtaatagac cttgctacgg ctcacaccca ataagcgtgc
     1321 cgcttccgtg acattcccac cggtgttttc aatcactttc ttgatgtatt cctgttccat
     1381 tttttgcatc acatttttga gcgattgatc aattttcgaa aacttgagtt ccggcacagc
     1441 ttcatagcta ttggggtcga gcacagactt ctgttctgtc gctgtcatcc attgcggtaa
     1501 atcagccaaa acgatcttgt cacccacagc catgatgatg agactctgca aaacattttc
     1561 gagttcgcga atattaccgg gccatttgta ttgcttgaaa aactccaaaa cggcagggga
     1621 caccaatttt ttttggtccg cgcgactgtg cttcttgata aaaaaagcca caagctcgtc
     1681 aatgtcctct tgtctttttc tgagcggcgg catcgtgagc gacaccacgc gcagtctgta
     1741 taacaagtcc tgtcgaaaat gattgttcct cacttcttcc tgaagatcga cattggtggc
     1801 cgcgatcacc ctgaacttga cttgaatcga ttgcgtgccc cccagacgat aaaattcttt
     1861 ttcctgcaac gccctgagga gcttggcctg cagttccggc cgcaggctgt tgatctcatc
     1921 gagaaagata tctccgccat tggccagttc gaacttaccc accttctttt tgctggcacc
     1981 tgtgaaggcc ccttgctcat aaccaaaaag ctccgattcc aaaaggttgt ctggaatggc
     2041 cgcacagttt acagcgataa atggtcgccg cacatcttct tcgagcccat gcaggtggcg
     2101 agccaataat tccttgcccg tgccactttc accttcgatg aggacagagg cattatagcc
     2161 tttaagacgc ttggcgctat caagcacatc cgttaacgat tttgattttc cgatgaatcc
     2221 tttaagggac tgacattcgt tcatgtcttc aatcagggcc tcataacgat ccttgatctc
     2281 ccggttcttg agctgttttt cgaccacagc cagcagatcc tcagacgggc aaggtttgca
     2341 tatgtaatca gaagcaccat ctctgatggc attcacaatg agttgtggat ctctttcgcc
     2401 agacaacatc acgacatcga ggttcggatg caattctttt attttataca ggagatctat
     2461 tccattctcc ttgcccagct tcacatccag aaacagcaaa tcgatcaact gtgtttcgag
     2521 aatcttcagc ccctcctcaa aagacaaggc tgttgtcacc atatactttt tcttgagcac
     2581 agattttgtt gccaacaaaa aactggggtc gtcatcaatg cacaaaatat taaataacaa
     2641 cattcgaacc tcctcattga atctatccgt cttcagagca aaacacatgc cattcttttt
     2701 ataataaaaa acgtcctgtt aatagctagt tgttcagatg tcattgcatc tgacacaaaa
     2761 tcagtccaaa tatttttaca attgtgttct aatattcgca catgtgtcca aatattagaa
     2821 catgtgccac atgtcaaggt cgtataaaca caaaatctac tacaaggtta aaaggagttt
     2881 ttctcaaaaa tagaatattg gcgttttttt gtcgttttga ttcacatacg caagcaaaaa
     2941 catgccggta aatcgattat tatacaacaa tatcaatctg ttgtgtttgt tgacacaaaa
     3001 atgtctgtgg caagcttctt gctagttaat agtaacgtgc gcatcatcta caacagagaa
     3061 gacccacagc acaacgattt caacataaac ctgctaaggg atttgaaggt attaacccaa
     3121 cctttgtcgg cggctgacca agccgccttc ctctcagaca ttaccgaatt tctgcaatca
     3181 tcgccatccc gtgacaacct tgtttttgag tctgcagaca aacttgccga ggccctcaag
     3241 caacatgtct ctcgttttgg gggcctgtat gttccaaaat attttcagca ggttgtcacg
     3301 caggcaaggc gccttgagga acacgtgttc gaaattcgcg ccgtggaggg cggtttgttt
     3361 tacaatttct atcctatcgg ccatcaacca ggccacctgg ccgacaacgc ctctgcccgt
     3421 agcaccgatg tatttgtgtg cccagaaaga tcgcgcacca tctacaccac caccgaattt
     3481 gatttttctg atatcccgta tgatttcttt ttccccccca atgccgttct taagtttaat
     3541 ggcaacgacg ttgtttccat catgtcctca ttgccccaga tagatcctaa agagagactg
     3601 tcggtcctgc aacgcatgat gtcactagca acaacttcat cggtcgccct tcaatcagac
     3661 gctcttcaat ctgatcacaa acagaatttt ctggcctatg ccgcactact gaaaagtctt
     3721 tccgggcatt tcaccctgac aaaggatctt gccatcagta cacttgagag ggtggaaaac
     3781 atgtatcagc gctgtcgcca tgaaatgcgg caccccgcct tttacgaaac aaccggcatc
     3841 acacaagcag agaccctgct gaagcaacat cccgaatttg gcaaagtggc ctacattgaa
     3901 ggcatcgccc agcacatcgc gcaggcgggt cctgccttcg agcttctggg ggccatcacc
     3961 cccgctgggc tggaaaaccc tga
//
LOCUS       DATDUN010000680         1217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_36880, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000680 DATDUN010000000
VERSION     DATDUN010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36880"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..584)
                     /locus_tag="VJC18_09525"
     CDS             complement(291..584)
                     /locus_tag="VJC18_09525"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLD45663.1"
                     /translation="MVDVAPHQLDLIKTILKKHIPDRSVWAFGSRVTGQANQHSDLDL
                     VILGAESVDTKTMAMLEYDFQISEIPFRVDLIQWATTSEPFRQVILKQYEKIV"
     gene            complement(577..1002)
                     /locus_tag="VJC18_09530"
     CDS             complement(577..1002)
                     /locus_tag="VJC18_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HLD45664.1"
                     /translation="MKKLLLTSLKKALDTLEKSLNITNSSERMKEMDEDLAETVRAGV
                     VQKFEVAYELCWKFMKRWLEENIGKTYVEGVPRYELFRLAAENKLIDNVEDWMQFHKL
                     RNETSHTYNVATAETVFNESAHFCLIAKDFFANLESKNG"
BASE COUNT          326 a          250 c          275 g          366 t
ORIGIN      
        1 agagaaacaa aacaaaacaa ggatgaaaac acgcaggaat ttattaatca tggctgcctc
       61 acaagttatg tcaacaccag acgatgacac cctgttatta gcaatatcca taccaatcgt
      121 tgatctgtta gttcgttgtt attgtaattg aatttttatt gttccgtgtt caaattgaga
      181 aaatggtggt agggttatga taaattttca aagtgataac aataatgtcg cagaaattat
      241 gggttatggg cgtaaggctt agggcttagg gcataatgct cagggaagga tcagacaatc
      301 ttttcatatt gcttaaggat tacctgtcta aagggttcag acgtggttgc ccactgaatg
      361 agatcaacac gaaagggaat ttccgaaatc tgaaaatcgt attcaagcat ggccatggtc
      421 ttggtatcaa cggactcagc tcccaaaatc accagatcca aatctgaatg ttgatttgcc
      481 tggcctgtca cacgagagcc aaacgcccaa acagaccggt cagggatatg ttttttgaga
      541 attgtcttga tgaggtccag ctgatggggg gcaacatcaa ccatttttgg attccagatt
      601 tgcgaaaaaa tctttggcta tgagacagaa atgagccgac tcgttgaaaa cagtttctgc
      661 tgtggccacg ttatacgtgt gcgaggtttc gtttctgagt ttgtgaaatt gcatccagtc
      721 ttcgacattg tcaatgagct tgttttctgc ggccaaacga aacagttcgt agcggggaac
      781 gccttctaca taagttttgc caatgttttc ttcgagccag cgtttcatga atttccagca
      841 aagctcatag gccacctcga atttttgcac cacaccggcg cgcactgttt cggctaggtc
      901 ttcatccatc tccttcatcc gttccgatga attggtgatg ttcagggatt tttccagtgt
      961 gtccaatgcc tttttaagac ttgtgagcaa taatttcttc atagcgtccc cccacttact
     1021 tattgaaaca tacaattacc cgctttgtgg tcgcaagcaa tatgttatcc tctccaaatt
     1081 tgaattttca aataatccct ctctggcaaa gtgggcaggg agggatgatc gaaactgggt
     1141 tcgaggctac ggacaaactt gattgttttg ttcgcgagtt tggcggcttg cagggtccat
     1201 tccctttgtt tggtgtt
//
LOCUS       DATDUN010000681         1292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_286826, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000681 DATDUN010000000
VERSION     DATDUN010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286826"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..885
                     /locus_tag="VJC18_09535"
     CDS             145..885
                     /locus_tag="VJC18_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HLD45665.1"
                     /translation="MDAYQKNRSFLKSTLWKDFHLFPSDQKKGLQEPPRQKPIVNSTE
                     FVELTDPKYIKLGQLPTRDAIARRKSRRVFSEQSLTLEELSYLLWATQGMREDGPRQF
                     RNVPSAGARHPFETYLFVQRVEALTKGFYRYLPLEHKLAFAFERTDAPLRLIDACFGQ
                     KSVGESAITFIWTAIPYRTEWRYHATAYKVIALDAGHLCQNLYLACESIGVGTCALAA
                     YDQDKMDILMGVDGEDEFVIYMAAVGKI"
     gene            complement(882..>1292)
                     /locus_tag="VJC18_09540"
     CDS             complement(882..>1292)
                     /locus_tag="VJC18_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45666.1"
                     /translation="ELYGLLSLDGAVDPSKIFSRDECHRIATAWQAFVAQNPKAKNDL
                     QTLFAFMGIAPPYPKYRGSHTMLDLSAEQFALGRVTGITNKAGLTIDPVYMFMRPFTE
                     GLDASPVFLRFPYEMSGGKELHPKDCCDEFGCPV"
BASE COUNT          329 a          333 c          322 g          308 t
ORIGIN      
        1 gtgagcaaaa aaccgctcaa gaaaacggag tcgttaaagt cgcgagtgac ggaagatcta
       61 actgctatca aaaaaaggcc ctctctggtg aggtcttttt tttatgcccc aagcgtcaga
      121 tatactacgg actgattagt acttatggac gcctatcaaa aaaaccgctc ctttttaaaa
      181 tcaaccctct ggaaagattt tcacctgttc ccctcggatc aaaaaaaagg cctgcaggaa
      241 ccgccccggc aaaaaccgat tgtaaacagc acggagtttg ttgaactcac cgaccccaag
      301 tacatcaagc tgggacagtt gcccaccaga gacgccatcg cgcgacgtaa aagccgccgt
      361 gttttttctg agcagtcctt gaccctggaa gagctctctt atctactctg ggccacgcaa
      421 gggatgaggg aagacggtcc cagacagttt cgcaacgtgc cttcagcggg cgcacgtcat
      481 ccgttcgaaa cttatttgtt cgtccaacgt gttgaggccc tgacaaaagg tttttatcgt
      541 tacttgcctc tggaacacaa actcgccttt gcctttgaac gcaccgatgc cccgcttcgt
      601 ttgatagatg cctgctttgg acaaaaatct gtgggtgagt ctgctatcac gtttatttgg
      661 accgcgattc cctatcgcac agaatggcgg tatcacgcca ccgcgtacaa ggtgatcgct
      721 ctggatgcgg gacacctgtg ccagaatttg tatctggctt gtgaatcaat cggggtgggc
      781 acctgcgcct tggccgcgta tgatcaggat aagatggaca tcctgatggg cgtggacggt
      841 gaggatgagt ttgtgattta tatggccgca gtggggaaaa tttaaaccgg acacccgaat
      901 tcgtcacaac aatccttggg gtgtagttct ttcccgccag acatctcgta gggaaatctc
      961 aaaaagacag gactcgcgtc cagcccttct gtaaaaggcc tcatgaacat gtacacagga
     1021 tcaatcgtta agcccgcttt atttgtgatg ccagtcacgc gccccagggc aaattgttcg
     1081 gccgataaat ccagcatggt atgactcccg cggtatttgg gatacggcgg ggcaatgccc
     1141 ataaaagcga acaaagtttg caggtcgttc ttggccttgg ggttttgggc cacaaaggcc
     1201 tgccaggcgg tagcgatgcg atgacattca tcgcgcgaaa aaatctttga gggatccact
     1261 gctccatcca aactcagcaa cccatacaat tc
//
LOCUS       DATDUN010000682         1149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_492726, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000682 DATDUN010000000
VERSION     DATDUN010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492726"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VJC18_09545"
     CDS             complement(<1..1046)
                     /locus_tag="VJC18_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45667.1"
                     /translation="MNGDNKKIFIIVWSLIMWVCLACTQTHSGTTVVSNTVPGTVPAY
                     VVLERVQADWTRVVVPAMAFGFSDSEVLQGSFSIGINDQLVQEQKGTSAYFTSLTETP
                     TINIMVTDVKNGDQITLVIYQLSGRVLTYEGSVSLAQDIRAYLVTDFEEGGAVDLGQG
                     MDLTHEARALTALDDGSLLIAAELRDQPEGAYLTNKFSVRRYTADGELDMTYASGLGV
                     SVVDLDGFFETADDTLSGAQYYFGTSGDLWVLGDVYSQESVGQHYVSVIHVTAEGVPD
                     ENFGTDGRITIHPGNLYMGQVVLSMADNSLYFSLNDVDAPNGEMYLARITSEGVWDTS
                     FGTDGLFPLGLTVI"
BASE COUNT          304 a          340 c          247 g          258 t
ORIGIN      
        1 taaatgactg tgagacccaa gggaaacaat ccgtccgtgc caaaggaagt gtcccacaca
       61 ccttcggagg tgatccgggc caggtacatt tcaccattcg gagcgtcaac atcgttcaat
      121 gagaaataga gagaattgtc agccatgctc aataccacct gccccatata aagattacca
      181 gggtgtatgg ttattcttcc atccgttccg aaattttcat cgggcacacc ctcagcggtc
      241 acatgaatca cactcacata atgttgccca accgattcct gtgaatagac atcgcccaag
      301 acccataagt caccagaggt cccaaaataa tattgcgccc ccgataacgt gtcgtccgcg
      361 gtttcaaaaa aaccgtctaa atcgacaaca ctcactccca aaccgctggc ataggtcatg
      421 tcgagttccc cgtccgccgt gtagcgacgc actgaaaact tgttggtcag ataagccccc
      481 tccggttgat cacgaagttc agcggcgatc aggagtgagc catcatccaa agcagtcaac
      541 gccctcgcct catgtgtcag atccatgccc tgccccagat ccacagcgcc cccctcttca
      601 aaatcggtca ccaaatacgc cctgatgtcc tgtgcaagag acacactgcc ttcgtaagtg
      661 agtactctgc cggatagctg ataaataacc aacgtgatct gatccccatt tttcacatct
      721 gtcaccatga tgttgatggt gggtgtttcg gttaacgatg taaaatacgc cgaagtgccc
      781 ttttgctcct gcaccagttg gtcattgatc ccgatcgaaa acgacccctg caacacttca
      841 ctgtcgctga agccaaaggc catagccggc accacgacac gggtccaatc cgcctgcacc
      901 cgctcgagca caacatacgc tggaaccgta ccgggtacgg tgttgctgac gacagtcgtc
      961 ccggaatgtg tctgggtaca ggccagacac acccacatga tcagagacca cacaatgata
     1021 aaaatctttt tgttgtcacc attcatcata cgcttttatt ccaccgggat tgtatcctgc
     1081 cacactgtca aaggcaagaa cacaagggac atcgccgtca tccgtcagtg attggagcgt
     1141 tttgatttg
//
LOCUS       DATDUN010000683         1489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_492776, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000683 DATDUN010000000
VERSION     DATDUN010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492776"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VJC18_09550"
     CDS             <1..236
                     /locus_tag="VJC18_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLD45668.1"
                     /translation="PHFKARGMFVPVDKRDGNTQRQIAFPVKFSGNGTDYKHVGPTPG
                     HDTRQVLAEAGFLSEEISEFFRTGVVAAGQKIY"
     gene            309..>1489
                     /locus_tag="VJC18_09555"
     CDS             309..>1489
                     /locus_tag="VJC18_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLD45669.1"
                     /translation="MFKDLFARKSVAMLEAEMKGENRLRRILGPVALTNLGIGCIIGT
                     GIFVLTGVAAHDKAGPVLMLSYVVAGITCVFAALCYAEFASMTPVAGSAYTYAYATLG
                     ELFAWIIGWDLVLEYAVASASVAHGWSHYFQNFLSIFGVSFPRVLQLAPFDFDPAVGT
                     LFATGTYLDVPAILITVVITYVLVKGIRESARLNAVMVFTKVAVVLFVIVLGAFYINP
                     DNWVPFAPHGWSGLSFFGKTILGQSGLNGQPLGMLAGAAVVFFAYIGFDSVSTHAEEA
                     INPQKDVPIGIIASLLICTVLYIAVTAVLTGMVPSSEISIDAPVADAFKQVGLPWAQF
                     IIALGALAGITSVLLVLLLSLPRVLLAMARDGMLPESFFGAVHPKYRTPWKSTILVGC
                     FV"
BASE COUNT          285 a          337 c          436 g          431 t
ORIGIN      
        1 acccccattt caaggctcgg ggcatgtttg tgcctgttga taaaagagat ggtaacacac
       61 agaggcagat cgcttttccg gtaaaatttt cgggaaacgg cactgattat aaacatgtcg
      121 ggccgactcc tggtcatgac acgaggcagg ttcttgccga agccggattt ttaagtgaag
      181 agatatcaga atttttcagg acaggagttg ttgctgctgg gcagaagatt tattgacagc
      241 cttaattaaa tacccacata ctgcctgtgt gatttctgaa tttcacaatt tgtgaaggag
      301 gagatattat gttcaaagac ctctttgctc gaaaatcggt ggcaatgctg gaggcagaaa
      361 tgaaaggtga gaatcgtttg cggcgcattc tgggccctgt ggcgctcacg aatttgggga
      421 taggttgcat catagggacg gggatctttg tgctcacagg tgtggcggcc cacgacaagg
      481 ccggccctgt gctcatgttg tcgtatgtgg tcgccgggat cacctgtgtg tttgccgccc
      541 tgtgttatgc cgaatttgcc tccatgacac cggttgccgg atctgcctac acctatgcct
      601 acgcgacgtt gggagagttg tttgcgtgga tcattggttg ggatttggtc ttggagtacg
      661 ctgtggcgtc agccagtgtg gcgcacgggt ggtcacatta ttttcagaac tttctttcca
      721 ttttcggcgt gtcttttccc cgtgtcttgc agttagcccc tttcgatttt gatcccgcag
      781 tgggcacctt gtttgcgaca gggacttatc tggatgtgcc ggccatcctg atcacggtcg
      841 tgatcacgta tgtattggtc aagggcattc gcgaaagtgc cagattgaac gcggtcatgg
      901 tgtttactaa agtggctgtg gtgctctttg tcattgtctt gggggctttt tacatcaacc
      961 ccgataattg ggtgccgttt gcccctcacg ggtggagcgg gctcagtttt tttggtaaaa
     1021 ccattctggg tcagagcggg ctcaacggac aacccttggg aatgttggcg ggagcggcgg
     1081 tggtgttttt tgcctacatt ggttttgact ccgtttcgac acatgccgag gaggccatca
     1141 acccgcaaaa agatgtgccc attggaatta tcgcatcgtt gttgatttgt acggtgcttt
     1201 acattgcagt caccgcggta ttgacgggca tggttccgtc cagcgaaatc agcattgatg
     1261 ccccagtggc cgatgccttc aaacaggttg ggttgccttg ggcacagttc atcattgcgc
     1321 ttggcgcgtt ggcggggatc acgtcggtgt tgttggttct cctgctcagt ttgccacggg
     1381 tcttgctcgc catggcccgg gatgggatgt tgccggagag tttttttggc gccgtgcatc
     1441 ccaagtatcg cacgccctgg aaatcaacga ttctcgtggg atgttttgt
//
LOCUS       DATDUN010000684         1530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_76237, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000684 DATDUN010000000
VERSION     DATDUN010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76237"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VJC18_09560"
     CDS             <1..353
                     /locus_tag="VJC18_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45670.1"
                     /translation="LETDCVNQDGDVLFHGQRTYTRTLPPPQSKQTMVLPRDMNLFGC
                     SANLTVYCHNDIPCWTATQLIVDPQTNQMIGTKRYGELHFSPNFWGIDSIAGIQYPPN
                     AVTWWFLLDWRLIQ"
     gene            517..1131
                     /locus_tag="VJC18_09565"
     CDS             517..1131
                     /locus_tag="VJC18_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45671.1"
                     /translation="MIKEINLNELRPNQLPHRPYAHPFDFKIDCDSAPHGVGRDPDSI
                     DGHHAIGWYEVYRDHKTNELFLVHCYDGERRGKSAHTDGHLQWVEEVWPKIIERTYID
                     AGSSKPFITLSSAEWAIMRKRVFHGWLDTMDRETTSAMRAKTEGCIGKICGIEVRVDP
                     QAPNPLGEPIDLESAHEETKRDPLVKELSDYFETLAAAHGYEDY"
BASE COUNT          400 a          458 c          331 g          341 t
ORIGIN      
        1 gccttgaaac agattgtgtg aatcaagatg gagatgtctt gtttcatgga caaagaactt
       61 atacgcgtac actccctccc ccgcagtcga aacaaacaat ggttctgcca agagacatga
      121 atctgttcgg ttgttccgca aatctgaccg tatattgtca caatgacata ccctgttgga
      181 ctgccacaca actcattgtc gatcctcaga ccaatcagat gattggaaca aaaagatacg
      241 gcgaactcca cttttccccc aacttctggg ggatcgattc catcgctggc atacagtatc
      301 caccaaacgc tgttacttgg tggtttctac tggactggcg attgattcaa tgacaaactc
      361 aattcccatt ttccactacc cgtcctggta tcagaccaag gcggtttttt ctttgaaaac
      421 cttggatttt tgtcattcat tggttgacaa acaggcgttt ttctggtatc tttgacctgt
      481 ttctgattca gatttctacc acccacggag acgaagatga tcaaggaaat caatctcaac
      541 gaactgcgtc ccaaccagct cccccaccgc ccctatgccc atccgttcga cttcaagatc
      601 gactgcgaca gcgcgccgca cggcgtcggt cgcgatccgg actcgatcga tggacatcat
      661 gcgatcggct ggtacgaggt ctaccgtgac cacaagacca acgaactgtt cctggtccac
      721 tgctacgacg gtgaacgcag agggaaatcc gctcacacgg acgggcactt gcaatgggtc
      781 gaggaagttt ggcccaaaat catcgaacgc acctacatcg acgccggaag cagcaagccc
      841 ttcatcactc tctcgagcgc tgaatgggcg atcatgcgaa agcgcgtctt ccacggctgg
      901 ctcgatacca tggatcgcga gaccacctct gcaatgcgcg ccaaaaccga aggctgcatc
      961 ggcaaaatct gcggcatcga agtccgcgtg gatccccaag cccccaaccc cctgggtgag
     1021 cccatcgatc tggagagtgc ccacgaggaa accaagcgcg accccctggt caaagagctg
     1081 tccgactact tcgaaaccct cgccgccgcc cacggttacg aggattactg acctctctgt
     1141 ccgccaagcc tccccaaagg aagcccggcg gattttttgt taagcaagtt aaacaaggtt
     1201 ttttattcat cttttttgaa aaaggaaagg tgccaaaaaa agaagaaaag aataatcccc
     1261 aaagaacgcc aagctttcaa agaaagcccg gcttgccttg ccttgctttg ctttgtcttc
     1321 aaaaaaaaca aaggggctcg atgccccttt gttttttaca accccctcac cccgccggcc
     1381 accctccccc accccaacca ccacccaccc aaggaaagtg cggatacaat ttttctcggt
     1441 cgccccgaga aaaattcgta tacgcaattt cctcacacca acgactggtc tgcctgttcc
     1501 gcctcagagc tgccgaataa cagaacaaat
//
LOCUS       DATDUN010000685         2507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_113437, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000685 DATDUN010000000
VERSION     DATDUN010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113437"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VJC18_09570"
     CDS             <1..267
                     /locus_tag="VJC18_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HLD45672.1"
                     /translation="CEALLMRPDIILLDNMTPAQIRQLVRMRNKSCDPKHRPLLEISG
                     GVTLKNINRYVRLGVERISIGALTHSVMAADVAMKITWIVNRRS"
     gene            467..1735
                     /gene="hisS"
                     /locus_tag="VJC18_09575"
     CDS             467..1735
                     /gene="hisS"
                     /locus_tag="VJC18_09575"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HLD45673.1"
                     /translation="MSTIQSITGMSDVLPNEVPLWQYLEKTARAHFEHYGFAEIRTPV
                     LEDTRLFSRGIGENSQVVQKEMYTFLDKGGDSVTMRPEGTASVMRAYLEHSLMARDPI
                     TKLYYQGPMFRYERPQKGRLRQFHQIGVELLGIDSPLADAEVVIMMDRLIGLLGLSQY
                     HLEVNSLGTLEERKPYLERLVAFLDQYKTQLCEDCQKRLVSNPLRILDCKIATCKSLA
                     EQAPTILDSLTDASKREFQQFQSALDRANVSYIVNPRIVRGLDYYEKTAFEFTSDQLG
                     SQSAFCAGGRYNRLAEELGGKSVPAVGFSLGCERIVLLLAERQQETTMKGELNGVYVI
                     PMSETCFGPCVELLQQLRDQGIKAEMDYAVKSMKSQMRRANKLDFRWAVIMGDDERQK
                     GVVALKNLENGEQTEVVGSQLAAKLAPGPV"
     gene            1800..2015
                     /locus_tag="VJC18_09580"
     CDS             1800..2015
                     /locus_tag="VJC18_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HLD45674.1"
                     /translation="MKVTAILPDEIVREALLLAKTKTITASLIKALTEWIAIQKVNRL
                     RKKLVSRPMEFRDAFSAKQIRQLNRKK"
     gene            2012..2392
                     /locus_tag="VJC18_09585"
     CDS             2012..2392
                     /locus_tag="VJC18_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLD45675.1"
                     /translation="MIIPDTSIWIEYFKQHEPYCRRFDLLLEGGHVVAVAPVFGELLQ
                     GVKNRREERIICDFWKVLPKIDEASLWIDAGLYSSQHQLVSRGVGLIDVYLIMACTHH
                     RYKLWTLDQKLQKVAGDEILLSGS"
BASE COUNT          644 a          572 c          662 g          629 t
ORIGIN      
        1 tgcgaggccc tgctcatgag gcccgatatc attttactcg ataacatgac acctgcacag
       61 attcgccagc tggtgcggat gagaaataaa tcatgtgacc caaagcacag gccattattg
      121 gaaatttccg gcggggtcac gctgaaaaat atcaatcgct atgtgcgact tggcgttgag
      181 cgcatctcga ttggggcgct cacacactcg gttatggcgg cggatgtggc gatgaagata
      241 acgtggatcg tgaatcgtag atcgtgaatc gtgaatcgtg gttcgaaaaa aaatgtcaat
      301 ggaggtcttt gcgtgcatcg gcttgtcgag agcgaagcag gccatcaatc gtattttaaa
      361 ttgcttcttt gccatgaacc tattaacgga tcatgagtcc tcgcaatgac aatccacgat
      421 ccacgatcta cgattcacta tccacgatcc atgaatggag tcaatcatgt ctacaattca
      481 atccatcacc gggatgagcg atgttttgcc caatgaggtg cctttgtggc aatacctcga
      541 aaaaacagcg cgcgcacatt ttgaacatta cggctttgcc gaaatccgaa cacccgtatt
      601 ggaagatacg cgattgttct cgcgaggtat tggcgaaaat tcgcaggtcg tccaaaaaga
      661 gatgtacacg tttttggata agggtggcga ctcggtcacc atgcgtcccg aaggcacggc
      721 cagtgtgatg cgcgcctatc tggaacattc cctcatggcc cgcgatccca tcaccaaact
      781 ttattatcag ggccccatgt ttcgttacga gcgcccgcaa aaggggcgtc tcagacagtt
      841 tcatcagatt ggggtcgagc tgttgggcat cgattccccc ttggccgatg ccgaggtggt
      901 catcatgatg gatcggctga ttgggctttt gggactcagt cagtatcatc tcgaagtcaa
      961 ttccctaggg actctggaag aacgcaaacc ttatttggag aggctggtgg cttttttgga
     1021 ccaatacaaa acacagctat gcgaagactg ccagaaaagg ctcgtcagca atcctttgcg
     1081 gattctcgat tgcaaaatag caacgtgcaa atcgcttgcc gagcaggccc caacaattct
     1141 ggattcgttg acagatgcct ccaagcgcga attccagcag tttcagtcag cgctcgatcg
     1201 ggccaacgtt tcgtacatcg ttaatccccg cattgtgaga gggctggatt attacgaaaa
     1261 gaccgccttt gaattcacgt cagatcaatt aggctcccaa tcggcgttct gtgcgggtgg
     1321 gcggtacaat cgattggccg aagaattggg aggcaagagt gttcctgccg tgggcttttc
     1381 tctcgggtgt gagcgcatcg tgcttttgct ggccgagcgt cagcaggaaa ccaccatgaa
     1441 gggagagctc aacggggtct acgtgattcc catgtctgag acctgttttg gtccctgtgt
     1501 ggaactcctg caacaactcc gtgatcaggg aatcaaggca gaaatggatt acgccgtcaa
     1561 atccatgaag tcccaaatgc gccgtgccaa caaactcgat tttcgctggg ccgtcattat
     1621 gggagatgac gaacgacaaa aaggtgtggt ggcgctcaaa aatctggaaa acggtgaaca
     1681 gacagaggtg gtggggtctc aattggcggc aaagttggca ccagggccag tgtgatgggg
     1741 aatttgtctt catcagaaca tggttgcatt atccgattat atatcgtata atcaatcgta
     1801 tgaaagtcac cgccatccta cccgacgaaa ttgtgaggga agctttgctt ttggcaaaga
     1861 ccaaaacgat tacagcatct ctcatcaagg ctctcaccga atggatcgcc atccaaaaag
     1921 tgaatagact cagaaaaaaa ctggtatcgc ggccaatgga attcagggac gcattttcgg
     1981 caaaacaaat tcgtcagctc aatcgtaaaa aatgatcatc cccgacacat ccatctggat
     2041 tgaatacttt aagcaacacg aaccgtattg taggcgcttt gatctgttgc ttgaaggtgg
     2101 tcacgttgtg gccgtcgctc ctgtgtttgg tgagcttttg cagggggtaa aaaatcggcg
     2161 cgaggagcgg atcatttgcg atttctggaa ggtgttgcca aagatagatg aggcgtcttt
     2221 gtggattgat gcggggttgt attcgtctca acatcaattg gtttcaaggg gggtcggttt
     2281 gattgatgtc tacctgatta tggcctgcac ccatcacagg tacaaactgt ggacgttgga
     2341 tcaaaaactc caaaaagtag ctggggacga aatcttattg agcggttcgt agtttgtttg
     2401 gtagtttttg gatgatgagg aaataaacgg atttgttatc acacggcttg aacctgccat
     2461 tcttcattca cccaaagtcc catgaccggt atgctggggg aaaggtt
//
LOCUS       DATDUN010000686         1721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_199506, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000686 DATDUN010000000
VERSION     DATDUN010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199506"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..944)
                     /locus_tag="VJC18_09590"
     CDS             complement(141..944)
                     /locus_tag="VJC18_09590"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006459052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HLD45676.1"
                     /translation="MTSHRRLGVNIDHIATIRQSRGVVYPDPVEALSILKSSGVDQVT
                     IHLREDRRHIQDHDLDRILALGTLPVNLEMAVTDEMVAIACQKKPSHATLVPERREEV
                     TTEGGLDCEGNLSKVQRAVSALQRAGIHTSLFIDPDLEQVTAAKDVGANAIEIHTGTY
                     CNVIEEFYGHWGHYHWQEDPVYSQKVSAQLAKIRGAAQSAHDGGLKVYAGHGLHTQNL
                     APITAIELIEEYNIGHAIIARAVFVGLKQAIEEIQELLKIESWRLCEEL"
     gene            complement(1058..>1721)
                     /locus_tag="VJC18_09595"
     CDS             complement(1058..>1721)
                     /locus_tag="VJC18_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HLD45677.1"
                     /translation="MVLKHQADIGIALDGDADRVVISDEKGRLVNGDQIIALCAVNYL
                     NKGCLANRTIVGTIMTNMGVERYLDHKGIKLVRTRVGDRYIIERMRQDGTELGGEPSG
                     HIIFHNHNTTGDGIIAALQVLSILKETRKPLSALVSEISLYPQVSTTLNVKEKRPIED
                     IPAIQTAIKVLNNNLKGNGRAVVRYSGTEPVLRMMIEGEDQHLITSELEKLLGVAQEN
                     LA"
BASE COUNT          415 a          480 c          378 g          448 t
ORIGIN      
        1 gataacacct gtcaaaccga caacatcacc tgaccttaag gtcacagcaa accttttggc
       61 aaaatcagaa agttcatcat aatgaatgat cgtggtgggc atagatagaa tatgacattg
      121 tgtactggat tgcttcgtcg ctaaagctcc tcgcaaagac gccaactttc gatcttcaac
      181 aattcctgaa tttcctcaat cgcctgtttc aaccccacaa agactgccct ggcgatgatg
      241 gcgtgcccaa tgttgtattc ctcaatgagc tcgatcgccg tgatgggggc caaattctgt
      301 gtgtgcaaac catgtccggc gtagaccttc aaaccaccgt cgtgagcgct ttgtgcggca
      361 cccctgatct tagccaattg cgcggatact ttttgggaat atacgggatc ttcctgccaa
      421 tgataatgac cccagtggcc ataaaattcc tcgatcacgt tgcaataggt tcccgtgtga
      481 atttcgatgg cattcgcgcc cacatctttg gccgctgtga cctgctccaa atccggatca
      541 ataaacaaac tggtgtgaat gcccgcacgc tgcaacgcgc tcactgccct ctggactttt
      601 gacagattgc cctcacaatc aagcccaccc tcagtggtca cctcttcacg tctttctgga
      661 accaaggtcg cgtgcgacgg tttcttctgg caggcaatag ccaccatctc atcggtgacc
      721 gccatttcga gattcacagg taaagtcccc agtgccaaaa ttctgtccag atcatggtct
      781 tgaatgtggc gccggtcttc cctaagatgt atggtgactt gatcgacacc actgcttttg
      841 aggatggaca gggcctcaac aggatcggga tacaccaccc cgcgcgactg gcgaatcgtg
      901 gcgatgtgat cgatattaac tccgagacga cgatgtgaag tcatacagtc tccagcaaac
      961 acaaatatct atttgctttg aataatgaat aacgaacaag gaatgtcgaa tttcgaaatt
     1021 ccttgttcat tgttcgatat tcgttattca cacgccctca tgccaaattc tcctgtgcca
     1081 ctcccaacag cttctcgagc tcgctcgtga tcaggtgttg atcttctccc tcaatcatca
     1141 tccgcagaac aggttctgtg cctgaatatc ggaccacagc gcggccatta cccttaagat
     1201 tgttgttcag aactttgatc gcagtctgga tagcgggaat gtcttcgatc ggcctctttt
     1261 ctttcacgtt caatgttgtg ctcacttgcg gatacaggga aatttcggag accaaagcac
     1321 tcaaaggttt tcgcgtctcc ttgaggatcg acaacacctg cagggccgcg atgatgccat
     1381 cacctgtcgt gttgtgattg tgaaaaataa tatgacccga tggttcccca cccaactcgg
     1441 tgccatcttg tcgcatccgt tcgatgatgt aacgatcacc cacgcgggta cgaaccagct
     1501 taatgccctt atgatccaga taccgttcta cgcccatatt ggtcatgatc gtccccacaa
     1561 tcgtacggtt tgccaaacac cctttgttca gataattcac agcacatagc gcaatgatct
     1621 gatcgccatt cacgagtcgg cccttttcgt cgctgatgac cacgcggtcg gcatcgccat
     1681 ccagggcaat gccaatgtcg gcctgatgct tgagcaccat c
//
LOCUS       DATDUN010000687         2957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_199595, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000687 DATDUN010000000
VERSION     DATDUN010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199595"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2957
                     /locus_tag="VJC18_09600"
     CDS             <1..>2957
                     /locus_tag="VJC18_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45678.1"
                     /translation="ATAGILFIYNGQRFCENRLLKSVLRLKAGTVMARVVTNPQRKSG
                     QLKIMVGHIVVESYDCAVSGLGHETLLRLPQETGVGIARDEVEVTQKEIALIKNWIEQ
                     CQDFTVLNSLYKLCKTWDARVNGDDLQQALKAQVRKVVEEAIDANPKLVLYPDEPGLR
                     SAFVLNDNVIYLQPELYPDTLWDRFAVPQEFVNGTCQSDSGLTQPLRIVLSSDLVDDY
                     HWSYQSGTLYLKPETYRARAGFVLSALFMIDGLSFKANWQSVLLRAAVATMPVPKQEA
                     NHSEVPVLETEQTKWRRSTVHNAYSTQARPTYKVSQLHTFQKALQDIENVGLDVMSAS
                     IPGTVRGVSLYGSDETRLLSFLQMQGEHLPAGFIRQYERSDYLHIMSPSVATLATQYG
                     REVWYRASAIDGAAEATKIIRFGERYFILWPYSPASRIVDQTITPVVLGSRLSNLREE
                     HALLVAENHDGGVLRYFLFDKNLQSVLESENIRSFLKIDANDGFVVETANGWLLLDDQ
                     NEVVFDGRGSDIRPLGMYHHTDGSQRFITVHKGQVQVIDGASNIQWQWQRPEESAGLA
                     TAKLDFVNDQTPLLVFGHLNGDKMEVFDLRTFELIAKDVTRYRTTHGIFVTHCDNRAL
                     VYDTDLKPVLDLVTHHCEALVHPNGRKVIMQNLQGEQHILYDEHFQPLLNTGELDFVD
                     DTDSEFMFFIRVAGGFRVLNLWDFELEEHVFTVPEKQLNLLDGPDRTTFIVVADPDQN
                     KLALHRPDGSLVATVADAQTIELLSLDGHYFYSVVTDSGVRILDVRFNELLAVPAENP
                     AKKADIFLIRHSQGIGLLCSSQYYHYDPETAAFRLMFSNVELLGKNDFTWLAAVHEGG
                     RTTFHSMDGQTLMTVDEDVMAVPVLHDQELLGIFFQQRTGQNQIAYFPLATWQEANRA
                     VPDRVAFVARVHGKKTGAIVPVLSDLSEALWMRFKDDAELLLEMGNADNRRFAQGHHE
                     LGSLQLKQSFELM"
BASE COUNT          753 a          576 c          786 g          842 t
ORIGIN      
        1 gcaacggcag ggatattgtt tatttataat ggtcagcggt tctgtgaaaa caggcttctc
       61 aaatctgttc tgcgattgaa agcgggcacg gtgatggcca gagtcgtaac caacccgcaa
      121 cgaaaatcgg ggcaactcaa aatcatggtt ggtcacattg tggttgaatc atacgattgt
      181 gctgtttctg gccttggcca tgaaaccttg ttgcgtctgc cacaggaaac aggcgtgggg
      241 attgcgcgag acgaggttga agtgacccag aaagaaattg ccttgatcaa aaactggatt
      301 gagcagtgcc aggattttac cgttttgaat tctttataca agctttgcaa aacctgggat
      361 gcccgggtga acggcgatga tttgcaacag gccttaaaag cgcaggtgag aaaggttgta
      421 gaagaggcga ttgatgcaaa tccaaaattg gtgctctatc ctgatgagcc aggtttacga
      481 tcggcttttg ttttgaatga taatgttatt tatttgcaac cagagttgta tcccgacact
      541 ttgtgggatc ggtttgccgt gccgcaggaa ttcgtgaacg gcacctgcca aagtgattct
      601 ggtttgacgc aacctctgcg gattgtttta tcatcagatc ttgtggatga ttatcattgg
      661 tcgtatcaat cgggaacatt gtatcttaaa cccgagacgt atcgtgcacg cgctggtttt
      721 gttctgtcgg cactgttcat gattgatgga ttgtctttta aagccaattg gcaaagtgtt
      781 ttgctgagag cggcagtagc aacaatgcct gttccaaaac aggaagccaa tcattctgaa
      841 gttccggttc tagaaacaga gcaaacgaag tggcgcagaa gcaccgtcca taatgcctat
      901 agtactcaag cgagaccaac ttataaagtg agtcaattgc acacatttca aaaagcgtta
      961 caggatattg aaaacgtagg gctcgacgtc atgagcgcca gcatacctgg taccgtgcgt
     1021 ggcgtgtcgt tatacggaag tgatgagact cggttgttat catttctaca aatgcagggt
     1081 gaacatttgc ctgctggctt tataagacaa tacgaacgct ctgattactt gcacatcatg
     1141 tcgccatcag tggcaacatt ggccactcaa tatgggagag aagtgtggta tcgagcgtca
     1201 gcgatcgatg gggctgctga agcaacaaaa atcattcgtt ttggagaacg ttattttatt
     1261 ttatggccct atagtcccgc gagcaggatt gttgatcaaa cgataacacc agtggttttg
     1321 ggtagccgat taagcaatct gcgtgaagaa catgcgcttc tggtggcaga aaatcacgat
     1381 ggtggtgttt tgcggtattt tctttttgat aaaaatctgc aatcagttct tgaatcggaa
     1441 aatatcagat cgtttctcaa gattgatgcc aatgacggat ttgttgttga gacagcaaat
     1501 ggctggctgt tgttagatga tcagaacgag gtggtgtttg atggacgcgg gagcgatatc
     1561 aggcccttgg gcatgtatca ccacaccgat ggcagtcagc gttttatcac tgttcacaaa
     1621 ggtcaggttc aggtgattga tggagcgagc aatattcagt ggcagtggca acgcccggag
     1681 gaatctgcgg gccttgctac ggcaaagttg gattttgtga atgatcaaac gccgctattg
     1741 gtttttggtc acttgaatgg cgacaagatg gaagttttcg atctcaggac ttttgaactc
     1801 attgccaaag atgtgacgcg ttaccgcacg actcacggta ttttcgttac ccattgtgat
     1861 aatcgggcgc tggtttatga taccgattta aaacctgttc ttgatttggt cacacatcat
     1921 tgcgaagctc ttgtgcatcc caacggcaga aaagtcatca tgcagaattt gcagggtgaa
     1981 caacacatcc tctacgatga acattttcag ccattgttaa acacaggaga gttggatttt
     2041 gttgacgaca ctgactcaga atttatgttt ttcattcgtg tagccggtgg gtttcgtgtc
     2101 ctgaaccttt gggattttga gttggaggaa catgtcttta ctgttccaga gaaacagttg
     2161 aatttattgg atggtcctga tcgaactaca ttcattgttg ttgctgatcc ggatcagaat
     2221 aaactggcgt tacaccgtcc ggatggaagt ttggttgcta cggttgccga tgctcaaaca
     2281 atagaattgt tgagcctgga tggtcattat ttttacagtg tggtgacgga cagcggtgtg
     2341 aggattctgg atgtgagatt taacgagctt cttgcagtcc ctgccgaaaa tcccgcaaag
     2401 aaagctgata tttttctgat tcgtcattcc caaggcatcg gcttgttgtg cagctcacag
     2461 tattatcact acgatcctga aacagcggca ttccgattga tgttttctaa cgtggaattg
     2521 ttgggtaaaa atgattttac ctggctggcg gctgtgcacg agggtggacg aacgacgttt
     2581 cattctatgg atgggcaaac tctcatgacc gtggatgagg atgtgatggc agtgccagtt
     2641 ttacatgatc aggagcttct ggggatcttt tttcaacaga gaactggtca aaatcagatc
     2701 gcgtattttc cgctggcgac ttggcaggag gcgaatcgag cagttccaga cagggtggcg
     2761 ttcgtggcga gagtccatgg gaaaaaaact ggcgctattg ttcctgtgct tagcgatcta
     2821 tctgaggcgt tgtggatgcg atttaaagac gatgccgaac tgcttttgga aatggggaac
     2881 gctgacaatc gtcgttttgc ccaggggcat catgagcttg ggtcgttgca gttgaaacaa
     2941 tcctttgaat tgatgag
//
LOCUS       DATDUN010000688         1750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_287333, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000688 DATDUN010000000
VERSION     DATDUN010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287333"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VJC18_09605"
     CDS             <1..301
                     /locus_tag="VJC18_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HLD45679.1"
                     /translation="GQNYRATGVILCDLKQDHPVQLGLFEEPLRIVKTEKVDQAIDQI
                     NQQFGRHTIHLASTLPVYGHHRDDRVVNGTEGVVRCGPIRGKGLAVPLLQVGHDK"
     gene            complement(309..1313)
                     /locus_tag="VJC18_09610"
     CDS             complement(309..1313)
                     /locus_tag="VJC18_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45680.1"
                     /translation="MIQKKQTFFLLMFASFTLLAFTQCGADRFAYDPVDSLATDDDQG
                     DEIDVAINESEDPVADPVDVSAEDTSTADPTSTNITPISLPAEENENGDGEKKKVNEP
                     ALAQETPIETDEADDVDLDPTGGVPTTVEAEETSPQFDAGLPSDAMQDRIKDYLNSSE
                     NPSDGISVMPYDGAARGTLTPAEEEDNGMMSHWNNNFEILKGAGIINSATNNTKTVPN
                     TGALQAFVDNVETSFSRSDYKANFPKDTYDNYFENDVQVKAVPVKEILEHFTEEDIAY
                     VVQSMPDTTTAEDWEYTDVPQDNPATTSYSADYVEYKSYKHGSVNSDTVYTADEDDDA
                     "
     gene            complement(1468..>1750)
                     /locus_tag="VJC18_09615"
     CDS             complement(1468..>1750)
                     /locus_tag="VJC18_09615"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF013606.4"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HLD45681.1"
                     /translation="DLLEQHGIAGMYERISRTAAYGGLTRGPQVIDQHVKDNMRKILT
                     HIQNKDFANEWIHECALGQPNLNQLHEQNRQLAIETTLRGLHPPIKRNM"
BASE COUNT          416 a          372 c          442 g          520 t
ORIGIN      
        1 agggcagaac tatcgcgcta cgggggtgat tctgtgcgat ctcaaacagg atcatcccgt
       61 gcaactgggt cttttcgaag agcctctccg cattgtcaaa acagaaaaag tcgatcaggc
      121 gatcgaccag atcaatcaac agtttggacg gcacaccatc catctggcca gcacgttgcc
      181 agtttatgga caccataggg atgacagagt tgtgaacggc acagaagggg ttgtcaggtg
      241 tggccctatc agggggaagg gtttggctgt gcctttatta caagtgggac atgacaaata
      301 aatcatggct aggcgtcatc atcttcatca gctgtgtaaa cagtgtcact gttgacactg
      361 ccgtgtttgt aagacttgta ttctacatag tccgctgagt aactggtggt agcagggttg
      421 tcttgtggga catcggtgta ttcccaatct tctgccgtgg ttgtgtcagg catgctctgc
      481 acaacatagg cgatgtcttc ttcggtaaag tgttccagaa tttctttgac cggcacggct
      541 ttgacttgca catcgttttc aaaataattg tcgtaggtgt cttttggaaa gttggctttg
      601 taatcggagc gtgaaaaaga agtttcgacg ttgtctacaa aagcctgcag ggccccggta
      661 ttggggactg tttttgtgtt gtttgttgca gaattgatga tgccggcacc tttcaaaatt
      721 tcgaagttgt tattccaatg ggacatcatg ccattatctt cttcttcagc tggagtgaga
      781 gtgcctcggg ctgcgccatc atagggcata acacttatgc catcggaggg gttttcggac
      841 gaattgagat agtccttgat gcgatcttgc atagcatctg atggcaggcc ggcgtcaaat
      901 tgaggactgg tttcttctgc ttcaaccgtt gtgggaacac caccagtcgg atctagatca
      961 acatcatctg cctcatctgt ttcgataggt gtttcctgtg ccagagcagg ttcattgact
     1021 ttcttttttt ctccgtctcc gttttcgttt tcttccgcag ggagagagat aggggtgatg
     1081 tttgtagagg tagggtctgc tgtcgaggtg tcttccgccg aaacgtccac tggatcagca
     1141 acggggtctt cactttcatt gatggccacg tcaatttcgt ctccctgatc atcatctgtt
     1201 gccaagctgt ccacaggatc ataggcaaac cggtcagcac cacattgcgt aaaggctaac
     1261 agagtaaatg aagcaaacat caaaaggaaa aaggtttgtt ttttctgaat cataatattc
     1321 tccctaacag cccttaatgc aatttcgttg ccaaacagaa aacaaacgat gcttttgatt
     1381 cattaaaatt gtgcaattat ttcaaagaaa tacaaaacat gtgtgattat cgttttgata
     1441 gaacccgttg ggagagggga ctatgcgtca catatttctc tttattggtg ggtgcagacc
     1501 acggagggtt gtttcgatgg caagttgtct gttttgctca tgcaattgat taaggttggg
     1561 ttgacccaag gcgcattcgt ggatccattc gttggcaaaa tctttgtttt gaatgtgggt
     1621 caggattttt cgcatattat ctttgacgtg ttggtcgata acttgcggtc cgcgtgtcaa
     1681 accgccgtaa gccgcagtac gagaaatgcg ttcatacatt cccgcaatcc cgtgttgctc
     1741 caaaagatct
//
LOCUS       DATDUN010000689         1073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_76575, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000689 DATDUN010000000
VERSION     DATDUN010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76575"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VJC18_09620"
     CDS             <1..557
                     /locus_tag="VJC18_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLD45682.1"
                     /translation="AGYYPNAEMLRTKLVYESSSGRLLGAQVIGCAGVDKRLDVLATA
                     IFAKLTVFDLEELDLCYAPPFGSANDPVNTAGFVAAHIVTNDVPNIKYEKDLPANAQL
                     IDVRNHAEIKASGLLAGAIAIPLPQLRDRWHELDQTKPVVVYCAKGLRGYLALRILKG
                     NGFANVSNINGGYPLAKINGWKTE"
     gene            618..>1073
                     /locus_tag="VJC18_09625"
     CDS             618..>1073
                     /locus_tag="VJC18_09625"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLD45683.1"
                     /translation="MYVLDTSKIHAAIKDKGFGSVSALANKLKVHRNTLQYYLSGAPI
                     INDKLDLVLNALKLELADVVIKTGQEADYPWEPIVGVVDELNKQFSVLTFVLFGSRAR
                     ESHRKYSDWDIGVYSPKGLVHKEFRKLVLLKSELVDPLPVSLDLVNLNSA"
BASE COUNT          282 a          235 c          260 g          296 t
ORIGIN      
        1 acgctgggta ttatcccaac gcagaaatgc ttcgcacgaa actcgtttac gaatcatctt
       61 cagggcgact cttgggtgca caagtgattg gttgtgctgg tgttgataaa cgtttggatg
      121 ttttggccac ggcgattttt gcaaaactga cagtttttga tttggaagaa ctcgatctct
      181 gctatgcccc accttttgga tctgcgaacg atcccgtaaa taccgcagga tttgtggcgg
      241 cacacattgt gaccaacgac gtgcccaaca tcaaatacga aaaggacttg cctgccaacg
      301 cacaactcat cgatgtgcga aaccatgccg aaatcaaagc cagtggcctt ctggcgggcg
      361 ccatcgctat tccgttgcca caactccgtg atcgttggca tgaactggat caaacgaaac
      421 ctgttgtcgt ttattgcgcg aagggtttgc ggggttatct tgctttacgc attttgaagg
      481 ggaacggttt tgcgaacgtt tccaacatca acggcggcta cccgctggcc aaaatcaatg
      541 ggtggaaaac cgaatagtgt tgaacagtat ttatgtgcaa ttgactaaaa tcattatgca
      601 tgatatgatg gaagtgtatg tatgttctcg atacctctaa aattcatgcg gccatcaaag
      661 acaagggttt tggttcggtc agtgccttgg caaacaaact taaagttcat cgcaacactt
      721 tacagtatta cttgtcggga gcacccatca tcaatgacaa acttgatctc gtattgaatg
      781 cactcaaact agaactggct gatgttgtca tcaagacagg tcaagaagca gattatccct
      841 gggaaccgat agtgggtgtt gtggacgaac tgaacaaaca attttctgtc ctcacatttg
      901 tgttgtttgg ttcccgagcc agggagtctc acagaaaata ttccgattgg gatattgggg
      961 tgtattctcc aaaaggactg gtgcataaag agtttcgcaa actggttttg ttgaaatcgg
     1021 aactggtaga cccgttgcct gtctcgctgg atttggtcaa tcttaacagc gcc
//
LOCUS       DATDUN010000690         1367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_368022, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000690 DATDUN010000000
VERSION     DATDUN010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368022"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VJC18_09630"
     CDS             complement(<1..218)
                     /locus_tag="VJC18_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45684.1"
                     /translation="MQKKYFWFMFFICVGLIHCGGGSDTNSSSPTDTTALEYATYGVA
                     LSQAIPDGLKQGGAELAGSLSALKKTLT"
     gene            complement(279..875)
                     /locus_tag="VJC18_09635"
     CDS             complement(279..875)
                     /locus_tag="VJC18_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45685.1"
                     /translation="MSHDRQKYLDAIKQQLEPNIVNHSMALEACMGGIYDHLQAVGQL
                     DAAELPKDDWLLAGLIHDIDFGGEFKDQHPHKTTEALAKYGLTISPAVLELVRSHAAR
                     ECVITLQSKAQWAIYCADSLTGLITAVAFVYPSKKLADVKLSSVVKRFLKEPRFAAGT
                     RRDEVALCAKADGLNIPLDKFIEICLVSMQKIGGELGL"
     gene            complement(917..>1367)
                     /locus_tag="VJC18_09640"
     CDS             complement(917..>1367)
                     /locus_tag="VJC18_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HLD45686.1"
                     /translation="VVDDDDDDDAGEEGADVEDEDPCAMQIYYQDSDDDSWGNTSVSK
                     TDCSPPADYVTFHGDCDDENKDAYPGATEICDGKDNDCDDEVDEGLMTSYYPDNDGDG
                     YGDKNATGSEIISACSPPANYVADRTDCCDTNATCHSGVVCAMRCSP"
BASE COUNT          338 a          374 c          292 g          363 t
ORIGIN      
        1 tctgtcagtg tttttttcag cgcgctcaga cttccggcaa gctctgcccc gccctgtttt
       61 agcccatccg gaatggcctg actcaaggcc acgccatatg tggcgtattc aagggccgtc
      121 gtgtcagtag gacttgaact gtttgtgtca gaccctcccc cacaatgaat caaacccaca
      181 caaataaaaa acatgaacca gaaatatttt ttttgcatac ccaccccctt ttttctttct
      241 gctaccgcta ataaaactga aaagccagca aaacaacctt acaacccaag ctccccccca
      301 atcttctgca tggagacaag gcaaatttcg atgaatttat cgagcgggat attgaggcca
      361 tctgctttgg cacaaagcgc tacttcatcg cggcgtgtcc ctgcggcaaa ccgcggttcc
      421 tttaagaatc tctttacgac cgaagacagc tttacatcgg ccagtttttt ggacgggtaa
      481 acaaaggcca cggcagtgat cagacctgtc agactatcgg cacaatagat ggcccactgg
      541 gcctttgact gcaatgtgat gacacactcc ctggcggcgt gtgaacgcac cagttccaaa
      601 acagcaggcg aaatcgtaag accatacttg gccaaggctt ctgttgtttt gtgaggatgc
      661 tggtctttga attcaccccc aaaatcgata tcgtgaatga gccctgcgag taaccaatca
      721 tccttgggga gttcggcggc gtccaattgc cccaccgctt gtagatgatc gtaaatacca
      781 cccatgcagg cttcgagtgc cattgagtga ttgacaatgt tgggttccag ttgttgcttg
      841 atggcgtcca gatatttttg acgatcgtgt gacatgatat ctccctttag ctatcaatgg
      901 gggcacccat ttgtttttac ggcgaacacc tcatcgcaca caccacaccg ctatggcaag
      961 ttgcgttcgt atcacaacaa tcagtgcggt ctgccacata gtttgcagga ggtgaacagg
     1021 cagaaatgat ttcggaacct gtggcatttt tgtccccata cccatcaccg tcgttatcag
     1081 ggtagtaact ggtcatcaat ccctcgtcga cttcatcatc acaatcattg tctttcccat
     1141 cacaaatttc agtggcaccg ggataggcat ccttgttttc atcgtcacaa tctccgtgga
     1201 aagtcacata gtcagctggc ggggaacagt ctgttttaga cacggatgta tttccccaac
     1261 tgtcatcatc tgagtcttga taataaattt gcatagcgca ggggtcttcg tcttccacat
     1321 ctgccccttc ttcgcccgca tcatcatcat catcatcatc aacaaca
//
LOCUS       DATDUN010000691         1457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_237403, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000691 DATDUN010000000
VERSION     DATDUN010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237403"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1457)
                     /locus_tag="VJC18_09645"
     CDS             complement(<1..>1457)
                     /locus_tag="VJC18_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45687.1"
                     /translation="PSHPFTGVVLVIDAKRSLKILNIPFYCDDKLVSRLKSYLLNESD
                     SKGKIKASIAETTGRLIFDPNVERQNTLKLTINSTNTNVSYDYIVQEDHLEFTMQVEG
                     NTSTVKLYPFEQRMIDALPPEFLPLGFKPTPHSVKPADESLATISPLVAEIPPEPISE
                     VEVTPAQKPVQTEEHIITQIWGPKTDLADPLQLILIRQRSAKGVVTAQKIRLVLHDFA
                     KELIGKDSLEQELQIGAETAVFNVGCLGAFEYALDLDRPDGHLAIREPKPITLKLDNL
                     HPDYPLLELYFGTNGENIVVDLQRINQDGQDHFFVCAGHLSLVQTVLECAGHPFPQAI
                     DAHMRFEVMTDDEGNVTLVQTPVVANQIGIQNIPLDKSTLDALDEASRPLEMAGTPVQ
                     GTSGVTEYEITVPLDVLFDFDENTNFVPGPQTLQILKTVFGEIALPDGTIVNVPRLVE
                     RQKLHYEIYDKTTGIILVLPMAWRGAGRPDAPFES"
BASE COUNT          373 a          316 c          353 g          415 t
ORIGIN      
        1 agattcgaac ggagcatcag gccgcccagc acctcgccac gccatgggca aaacaagaat
       61 gatgccagtc gttttatcgt aaatttcgta atgtaatttt tgtctttcga ccaaacgtgg
      121 cacgttaaca attgtcccat cgggtaatgc gatttcacca aagaccgttt tcaatatttg
      181 cagtgtttgt ggccccggca caaagtttgt gttttcatca aaatcaaaaa ggacatccag
      241 gggcactgtg atttcgtatt cagtcacccc agatgtgcct tgcacaggtg tgccggccat
      301 ttcaagaggg cgactggctt cgtcaagagc gtccaaggtt gatttgtcca ggggaatgtt
      361 ctgaattcca atttgattgg caacaaccgg agtctgaacc agagtgacat tgccctcatc
      421 gtctgtcatc acttcaaagc gcatatgggc atcgatagcc tgtggaaaag gatgtcctgc
      481 gcattccaga acggtctgca caagcgaaag atgcccggca caaacaaaga aatgatcttg
      541 gccatcctga ttgatgcgtt gcaggtcaac gacaatattt tccccattgg ttccaaagta
      601 gagttcaagc agaggataat ccgggtgcag gttatcgagt ttcaaggtga tgggtttggg
      661 ttcgcgtatg gccagatgcc catcaggtcg atccagatcg agcgcatact caaaagcacc
      721 caaacagcca acgttgaaga cggcagtttc agcaccaatc tgaagttcct gttccaacga
      781 gtctttgccg atcaactctt tggcaaagtc gtgtaacacg agcctgattt tttgcgctgt
      841 cacaacaccc ttcgcagatc tttggcgaat gagtatcaac tgtaagggat cggcaagatc
      901 cgtttttgga ccccagattt gtgtgatgat atgttcttct gtttgaactg gtttttgtgc
      961 gggtgtgact tcaacttcgc tgatgggttc tggtggtatt tctgcaacca aaggggagat
     1021 agtcgcaagg ctctcgtctg ctggtttgac agaatgtggt gtgggtttaa accccagtgg
     1081 caaaaattct ggcggcaggg catcaatcat tctttgctca aaaggataga gtttgacagt
     1141 gcttgtgttt ccctcaacct gcatcgtaaa ttcaagatgg tcttcctgca cgatataatc
     1201 ataagacacg ttggtgttgg tactatttat tgtgagttta agggtatttt gtctttccac
     1261 atttggatca aaaatgagtc taccggttgt ttctgcaatg ctggctttga tttttccttt
     1321 tgagtctgac tcgttcaaca gataactttt gagacgagat acaagtttgt catcacaata
     1381 aaacgggata ttcaatattt taagactgcg tttggcatcg attaccagaa ccacaccggt
     1441 aaatggatga gagggta
//
LOCUS       DATDUN010000692         1558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_568821, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000692 DATDUN010000000
VERSION     DATDUN010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568821"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VJC18_09650"
     CDS             <1..386
                     /locus_tag="VJC18_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45688.1"
                     /translation="EGTGKYVSTLDDPLGLHTSERLVSVGDVDRDGHNDIVISFGQGT
                     VSRVFVHDAFGALQQSFITYPIDFLGGVQTVVGDLDQNGESEIYTIPVSSGGPHVRVF
                     NKNGDVIGGFFVGDESSRFGGTIAL"
     gene            557..>1558
                     /locus_tag="VJC18_09655"
     CDS             557..>1558
                     /locus_tag="VJC18_09655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HLD45689.1"
                     /translation="MAVLDTGVDLDHEDLEEHIWINEEEKMGDGIDNDDNGYIDDVYG
                     WDFVGEDATPTPDVEEGFDEGSVPHGTMIAGVIAAVTDNDKGVAGMSWNSKIMALRVM
                     DNYGAGLSTDVTEAIEYAVAQGAKIINLSFSGYDADNRLRAAVREAYEAGVLVVAAVG
                     NLSSGGVNTNVQPVYPACFGSQSDDDWVLGVAATNAEDEKTIFSNYGSTCVDISAPGE
                     NFYSTYYFDEDWEGFDEEEYMGQWSGTSLAAPVISGAAALLWSYYPSLTVGEIQTILK
                     ISVDPVVESGPIKHADMGAGRVNVERAFEIAANFAEPVSSVSLDEVTIIPSTNIIVVP
                     "
BASE COUNT          376 a          259 c          466 g          457 t
ORIGIN      
        1 acgaagggac aggaaagtat gtgtccacgc tcgatgatcc tctggggtta cacacaagcg
       61 agcgtttggt gagcgtggga gacgtggatc gagacggaca caatgatatt gtgatttctt
      121 ttggtcaggg aacggtttca cgcgtgtttg tgcatgatgc gtttggagcg ctgcagcaat
      181 ctttcattac ctatcccatc gattttttag gcggggtgca gacagttgtg ggtgatcttg
      241 atcaaaatgg ggagagtgaa atttacacga ttcccgtttc aagcggagga ccgcatgtcc
      301 gagtgtttaa taaaaacggt gatgtcatcg gtggattttt tgtgggagat gagtcatccc
      361 gtttcggcgg aaccattgca ttataagtat gaagtatttt caaaaaagtt tttttcttct
      421 tgtgattttt tttcttgtgg cacatcctgt cttcgctcaa gtgcccaatg atttttatta
      481 tctcgatcaa tggtatttga attttatcgg tgtttcaaac gtgtgggata cgacgacagg
      541 atccgatgag atcatagtgg cggttttaga tacaggtgtg gatttggatc atgaggattt
      601 ggaagagcat atatggatta atgaagaaga aaaaatgggg gacggaatcg ataatgatga
      661 caatggatat attgatgatg tctatggttg ggattttgtg ggagaagacg caacgcctac
      721 tccggacgtg gaagaagggt ttgatgaggg ttcggttcct catggcacga tgattgcagg
      781 tgtcattgca gctgtgacag ataatgacaa gggggtggcg ggtatgagtt ggaattcaaa
      841 aattatggcg ttgcgcgtga tggataatta tggagccgga ttatccacag atgtaaccga
      901 ggcgattgag tatgctgtgg cacagggggc gaagatcatt aatttgagtt tttccgggta
      961 tgacgcagat aaccgtttgc gtgcagctgt gcgcgaggcg tatgaggccg gtgttttggt
     1021 ggtcgctgcg gtcggtaatt taagttcggg tggtgtaaac acaaatgtac aacccgtcta
     1081 ccccgcatgt tttggaagtc aaagtgatga tgattgggta ctgggagttg cggctacaaa
     1141 tgcagaggat gagaagacga tattttccaa ttatggttcc acctgtgtgg atatttccgc
     1201 acccggagag aatttttatt ccacctatta ttttgatgag gattgggaag ggtttgatga
     1261 agaagaatac atggggcagt ggtcaggaac ctctttggcc gcacctgtga tttccggggc
     1321 cgccgcgctt ttgtggagtt attatcccag tctcactgtg ggggagatac agactatttt
     1381 gaaaatatcc gtggatcctg tggtggagtc gggtccgatt aaacacgcag atatgggggc
     1441 agggcgtgtg aatgtggagc gtgcgtttga gattgcggca aattttgccg agcccgtttc
     1501 ttcggtcagt ttggatgagg tcaccattat tccaagcaca aatattattg tcgtgccg
//
LOCUS       DATDUN010000693         1206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_528379, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000693 DATDUN010000000
VERSION     DATDUN010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528379"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VJC18_09660"
     CDS             complement(<1..629)
                     /locus_tag="VJC18_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HLD45690.1"
                     /translation="MKKILFIALICIVTSCSSGEAPSARQDQNSLSHTASQVAVNDDL
                     RKQAQAFIDVYTIEYLQHFYKLQIADWNSQTNINDENDKKKIEADTTLSLFVGNPETI
                     KKITYYLEKKDQLSDLQIRQLRYMKRKAAARPGTLPATVSQLIQSETKQISDLYGFNF
                     RVSERGQEKVVTANDIQTVLETAKDLKQRQLYWEASKEVGVVLKEGLDK"
     gene            787..>1206
                     /locus_tag="VJC18_09665"
     CDS             787..>1206
                     /locus_tag="VJC18_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45691.1"
                     /translation="MRHRSINTLTLFFVVTIVWIGLSATAFSTTINVNTLSEEAESGD
                     ELCTLTEAINAANSDTAVDCEAGSGADTIELPAGIYVTAIVAEYAYPEINSTITIHGN
                     GTNPEDTHLTRDSSDYVSFFSVAGSGNLTLQNIKLSDG"
BASE COUNT          289 a          297 c          277 g          343 t
ORIGIN      
        1 aatttgtcca aaccttcttt taaaacgacg cccacctctt tggaggcctc ccaatacaat
       61 tgacgctgtt tgagatcctt tgccgtttcc aaaacggtct ggatgtcgtt ggctgtcacc
      121 actttttcct ggccacgctc actcaccctg aagttgaatc catagagatc gctgatctgt
      181 tttgtttccg attgaatcaa ctgactcacg gtcgcgggca gggtcccggg tctggccgcg
      241 gccttgcgct tcatgtagcg gagctggcgg atttgcagat cgctcagttg atcctttttt
      301 tcgagataat aagtgatttt tttgatggtt tcgggattgc ccacaaacag cgacaaagtt
      361 gtgtcggctt cgatcttttt cttgtcgttc tcgtcgttga tgttcgtttg cgagttccaa
      421 tcggcgatct gtaatttgta aaagtgttgg agatactcta tggtgtagac atctataaac
      481 gcctgcgcct gttttcgcag atcgtcatta acagcaacct gactcgctgt gtgagacaag
      541 gagttctggt cctgtcgtgc cgatggtgcc tcaccagatg aacagcttgt gacaatacaa
      601 atcagggcga taaataaaat ctttttcata tttcctccga tcggaggact tataataaag
      661 attttgtagc gtcaaaggtt atgatgggga acccgtgtcc tgcaggacac ggttttttcg
      721 tcaacactca atattatcaa tcaggccacg ttcctttatg ataaaatacg acaaggagcg
      781 tgacctatgc gacacagatc gatcaatacc ctaaccctgt tttttgttgt cacgattgta
      841 tggattggct tgtcggcaac agccttttcc accacgatca atgtaaacac gctttccgaa
      901 gaggccgaat ccggcgacga attgtgtacg ctgaccgagg ccatcaacgc ggccaattcc
      961 gacacagctg ttgattgcga ggcaggatca ggtgccgaca cgatcgagct tcccgccggt
     1021 atctatgtta cagccattgt ggcagaatat gcctatcccg aaatcaacag cacgatcacc
     1081 attcatggca atggaacaaa tccagaagac acacacctca cacgcgattc atctgattat
     1141 gtgagttttt tttctgtcgc tggttccggc aacctgaccc tgcagaatat caaactcagc
     1201 gatgga
//
LOCUS       DATDUN010000694         1057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_287620, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000694 DATDUN010000000
VERSION     DATDUN010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287620"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..319
                     /locus_tag="VJC18_09670"
     CDS             155..319
                     /locus_tag="VJC18_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45692.1"
                     /translation="MSRYVIPATLRQALRVVSKVEPKQKSLLSTVLDPSFRWDDKPFY
                     DPQSTIHDSR"
     gene            323..>1057
                     /locus_tag="VJC18_09675"
     CDS             323..>1057
                     /locus_tag="VJC18_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLD45693.1"
                     /translation="MKFSDEQISSIVDKVVSQIQVKDTPPAGRPANTTATVSKQEPKK
                     DAAFTGSRGCFGSIDDAIAASVKALDDFKQISLEQRKRIIQKIRDLCKANVELLSKMA
                     REETGLGRVEDKIKKNLLAINKTPGVEDIEPQTFTGDHGFTLLEKAPWGVIGSITPCT
                     NPTETIICNGIGMIAAGNTVVFNPHPTAKKISVFVIDLMNQAVISEGGPPNLFTTIAT
                     PTIASANQLMNHKDIKLLVVTGGPAVV"
BASE COUNT          296 a          300 c          248 g          213 t
ORIGIN      
        1 ggccagaatg acacaagcaa ccgaaaacaa accggtcgat gccgtcatca tggccattgt
       61 cgatattttg gaaatcgatg gcgacaaaag gtatgtgaag aatccgtaat atcgtgggtt
      121 gtgggtcgtc gatcgtggat cgaaatgcga attgttgagt aggtatgtca ttcctgcgac
      181 ccttcgacag gcgctcaggg tggtgagcaa agtcgaacca aagcagaaat ccctactgtc
      241 aacagttctg gatcccagct tccgctggga tgacaaacca ttttacgatc cacaatccac
      301 gattcacgat tcacgctgac ctatgaaatt ctccgacgaa caaatcagtt ccattgtcga
      361 taaggtggtg tctcaaattc aggtcaaaga cacgccgcct gctggacgtc ctgcgaacac
      421 aacagcgacc gtctccaaac aggaacccaa aaaagacgcc gcgttcactg gttcccgtgg
      481 ctgttttggt tcgatcgacg acgccatcgc ggccagcgtc aaggctctgg acgatttcaa
      541 acaaatctca ctcgaacaac gcaaacgcat catccaaaaa atcagggacc tctgcaaagc
      601 caatgtggaa ctgctctcaa agatggcccg tgaagaaacc ggtctgggca gagtcgaaga
      661 caagatcaaa aaaaatctgt tggccatcaa caaaacaccg ggcgttgaag acatcgagcc
      721 acaaaccttc acaggtgatc acgggtttac ccttttggaa aaggcccctt ggggtgtgat
      781 cgggtccatc actccctgca ccaaccccac agaaaccatc atctgcaacg ggatcggcat
      841 gatcgccgca ggcaacacgg tggttttcaa cccgcacccc acagccaaaa aaatctcggt
      901 ctttgtgatt gacctcatga accaggccgt catcagtgaa ggcggccccc ccaatttatt
      961 caccaccatc gcgaccccca ccattgcctc ggccaaccag ctcatgaatc acaaggacat
     1021 taaacttctg gtggtgaccg gcggacccgc tgtggtc
//
LOCUS       DATDUN010000695         1119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_199778, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000695 DATDUN010000000
VERSION     DATDUN010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199778"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VJC18_09680"
     CDS             <1..693
                     /locus_tag="VJC18_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HLD45694.1"
                     /translation="PYLDDIQPNLPLVLANGSAVLTENGNQVITQSTLTKNTLELLLA
                     NQNALDGLQGIFLRYLNHTISDRATPQVKTYATTAKFTINETCRIEECVLKQMENNSN
                     LAPLKVELLVAPEEFARVVDKLKNVLSKQATVVLTNPSAAVVEIVPVGINKQVAIEKV
                     VSQKGFTLNDVVYFGDSQNDAEILGAVSLGIAMQNCHPDSCQAATFVTGNNDTDAIAN
                     VIKRLIIKESCQ"
     gene            complement(682..>1119)
                     /locus_tag="VJC18_09685"
     CDS             complement(682..>1119)
                     /locus_tag="VJC18_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45695.1"
                     /translation="KEIQLKHLPMNQTRALSVSPSTSTNSLFDADELKELSSLRKHGF
                     NFTLAEKELGYASNAKTLTNHFRGICYKMLALQEGPVSANDMFSMAQTVVGSADNQHL
                     IRKIGNKLERFYTRLKETIPANKEILLVNLPKKYWGYVEQLLT"
BASE COUNT          297 a          244 c          270 g          308 t
ORIGIN      
        1 ccctatcttg atgatatcca gcccaatctt cccttggtac ttgcgaacgg atcagctgtt
       61 cttacggaaa atggcaatca ggtcattacg caatccacat taacgaaaaa taccctcgaa
      121 ctgttgctcg ccaatcaaaa tgcgcttgac ggattacagg gaatattttt gcgatacttg
      181 aatcacacaa tcagtgatcg tgcaacacca caggtcaaga cgtacgcgac cacagcgaag
      241 tttacaatta atgaaacgtg cagaattgaa gaatgcgtcc taaaacaaat ggaaaacaat
      301 tcgaacctgg ccccattaaa agtggaactg ttggttgcgc cagaagaatt tgccagagtg
      361 gtcgacaaac tcaaaaacgt actgagcaag caggccacgg tagtattgac taatccgtct
      421 gcggctgtag tcgaaattgt gcccgttggg atcaataaac aggtggctat tgagaaggtt
      481 gtttcccaaa aaggttttac tctcaacgac gttgtgtact ttggagatag ccagaacgat
      541 gctgagattc tgggagcggt ttctttggga attgccatgc agaattgtca tcccgattca
      601 tgtcaggcgg ctacttttgt gacgggtaac aatgatacag acgcgattgc caacgtcatc
      661 aagaggctta tcatcaaaga atcatgtcag tagttgttcc acgtaccccc agtatttctt
      721 tggcaggttg acgagcagga tttccttgtt tgccggtatt gtctctttga gccgcgtgta
      781 aaaccgctcc agtttgttgc caatctttct gatgaggtgt tgattgtcag cggaaccgac
      841 aaccgtttgc gccattgaga acatgtcgtt tgcgctcaca ggcccctctt gcaaagcgag
      901 catcttatag caaattcccc taaaatgatt ggtgagtgtc ttggcgttgc tggcataacc
      961 cagttctttt tcggccagag taaaattgaa gccgtgtttg cgaagggagg agagttcctt
     1021 taattcatcg gcatcaaaca gactatttgt gctcgtggaa gggctgacgg acagtgctcg
     1081 tgtctgattc attggcaggt gtttgagttg aatttcttt
//
LOCUS       DATDUN010000696         1126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_614044, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000696 DATDUN010000000
VERSION     DATDUN010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614044"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VJC18_09690"
     CDS             complement(<1..774)
                     /locus_tag="VJC18_09690"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HLD45696.1"
                     /translation="MTLQQKRIFWIMADSLGVGEMPDAPFFKPVAGGDVGSNTLYHII
                     DKLGGLNALNLQQMGLANTLVCPEHPQGHLLWKPVSTPLASWGRMAEVSAGKDTPSGH
                     WEMAGCPVPFQMPVFDEGLPQEMLDRFLTRCREQGNPLPGVLGEGKPASGTEIIAELG
                     EEHVRTGKPIVYTSADSVFQIAAHEEAFGLQKLYDVCVIARAMLNEYEDKLGRVIARP
                     FVGHNKTDFKRTPNRHDYSLKPLKPTVLKAAKEAGYEVIS"
     gene            complement(812..>1126)
                     /locus_tag="VJC18_09695"
     CDS             complement(812..>1126)
                     /locus_tag="VJC18_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45697.1"
                     /translation="KNGKAHEALVAFLGELLGVDFRQGSVGVALHAYEEAMLPNSTYD
                     AKVAGLGSLRLDLFAGDGISCAVAFEPSERGYLPEVTLAFLNGLYEKLRGLEQWEPSH
                     QI"
BASE COUNT          281 a          323 c          273 g          249 t
ORIGIN      
        1 actgatcacc tcgtaacctg cctcctttgc ggccttgaga acagtgggct tgagtggttt
       61 gagcgaataa tcgtgacgat tgggtgttcg tttgaaatcc gtcttgttat gcccaacaaa
      121 cggacgagcg atcacgcgac caagtttgtc ctcgtattca ttgagcatgg cgcgagcgat
      181 gacacaaaca tcatagagtt tctgcaggcc aaacgcctct tcgtgcgccg caatctgaaa
      241 cacggaatcg gcgctggtgt aaacgatcgg tttgcccgtg cgcacatgtt cttcgccaag
      301 ttcggcgata atttctgtgc ctgaggcagg ttttccctct cccaagacac caggcagggg
      361 attcccctgc tcgcgacaac gagtcaaaaa ccgatcaagc atttcttggg gcaagccctc
      421 atcaaacacc ggcatttgaa aaggtacggg gcagcctgcc atttcccaat ggccagacgg
      481 cgtgtctttg ccggcagaga cctctgccat gcgcccccac gaagccaagg gtgtcgacac
      541 aggtttccac agcagatgtc cttgcgggtg ttcggggcaa accagagtat tggcgagccc
      601 catttgttga aggttgagcg cgtttaaccc acccaacttg tcgatgatat gataaagcgt
      661 gttagagcca acatcaccgc ctgcaacggg cttgaaaaaa ggagcatcgg gcatctctcc
      721 cacacccagc gaatccgcca tgatccagaa aatgcgtttt tgttgtagtg tcatgctcca
      781 ccggattaac ccgactcaac aggcagtgca atcaaatttg atgagacggc tcccactgtt
      841 ccaagcctct gagtttttcg tagagaccat tcaaaaatgc caaagtaact tctggaagat
      901 aaccgcgctc tgatggctca aaggcaaccg cacaggaaat gccatcccca gcaaacagat
      961 caagacgcaa cgaaccaagc cccgccactt tggcgtcgta tgtgctgtta ggcaacatag
     1021 cctcttcgta cgcgtgcaaa gctacgccaa ccgagccctg tctaaagtct acccccaaga
     1081 gttcccccaa aaacgcaacc aacgcctcgt gggctttgcc attttt
//
LOCUS       DATDUN010000697         1335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_493779, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000697 DATDUN010000000
VERSION     DATDUN010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493779"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VJC18_09700"
     CDS             complement(<1..1162)
                     /locus_tag="VJC18_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45698.1"
                     /translation="MTENESMIDFGQMSDTSKMDTPTVQPKVQLPLTTTLLQVFLLVT
                     TIALLHFASLSYPLTADSPDIINNSVLQSYVQDLKDPANYSQPYSIIKLSYIIDYALF
                     GTSASGFRLVNVLLHTLNTILLFFFLFLLLINVSQEQEGKQELSTLSWASFCVALLFA
                     VHPLSVFATTYVIQRPILLATFYCLLSLLAFFQGVLKQKFVWFFLSALAYGFALYSSE
                     RALFFMFVPLLFLPLIVTAEKGRASYVFVMILINLLTAIQFTASQWSGLALVHEPWMN
                     KLVAYASPDIANKLFSASLDSASYHMSLLQQAWLYFRYLFLSFFPDLKQLTVLVPISP
                     PTSWFSGPELFGCLLFVLYPIGPIKLISRKGFLGIVGAALFIPWILFIPELTI"
BASE COUNT          379 a          264 c          409 g          283 t
ORIGIN      
        1 tgatcgtcag ttcgggaata aacagaatcc acgggatgaa aagggccgcg cccacaatac
       61 ccagaaatcc ctttcgtgag atgagtttga tgggaccgat ggggtagagg acaaacagca
      121 ggcacccaaa aagttccggc ccgctgaacc atgatgttgg tggactgatg gggacgagga
      181 ccgtgagttg tttgaggtcg ggaaagaacg agaggaacaa ataacgaaaa tacaaccatg
      241 cctgttgcaa gagactcatg tggtacgatg cggagtccag actggccgaa aacagcttgt
      301 tcgcgatatc gggactggcg taggccacga gtttattcat ccagggttca tgcacgaggg
      361 caagtccgct ccattgggag gctgtgaact gaatagcagt cagcagattg attaaaatca
      421 ttacaaaaac gtatgaagca cgtccttttt cggcggtgac gatgaggggc aaaaaaagca
      481 ggggcacaaa catgaagaac agggccctct cggaggagta aagggcaaac ccgtatgcga
      541 gggcggagag aaaaaaccag acgaactttt gtttgaggac cccctgaaaa aaggcgagca
      601 gggagagcag acaatagaaa gtggccagca ggatagggcg ctggatcacg taggttgtgg
      661 cgaagacgct gagcgggtgt acagcgaaca acagggccac gcagaaagag gcccacgata
      721 gggtggacaa ttcctgcttg ccctcctgtt cctgactcac attgatgagg agcaggaaca
      781 aaaagaaaaa caacagaatc gtgttgagcg tatggagcaa cacgttgacc agacgaaacc
      841 cagaggcgga tgtgccgaag agggcgtaat cgatgatgta gctgagcttg atgatgctgt
      901 agggttgcga atagttggca ggatctttaa ggtcttgcac atatgattgc aggacagagt
      961 tgttgatgat gtcgggggaa tcggctgtga gggggtacga cagggaggca aaatgcagga
     1021 gagcaatcgt ggtgacgagc aggaacacct gcaagagcgt ggtggtcagg gggagctgca
     1081 ctttgggttg cactgtgggg gtatccattt ttgatgtgtc cgacatctgc ccaaagtcaa
     1141 tcatggattc attttcggtc attgcgcgtt tataaatgaa actgtaatat attcaattga
     1201 tatttgaatt gtgatctatc agcgtcagtc tcagttggga aaggtattgt tttttcgccg
     1261 agcgccacct gcgcctctgc gcagagtggc gcacattgat tttttgacat gtttttcggg
     1321 gacaaaaaat ctgct
//
LOCUS       DATDUN010000698         4156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_200092, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000698 DATDUN010000000
VERSION     DATDUN010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200092"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1966
                     /locus_tag="VJC18_09705"
     CDS             95..1966
                     /locus_tag="VJC18_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45699.1"
                     /translation="MNYTQTLQTKTDDLAKHISNCVQAISDQAFRESLAQRLIETLHH
                     AVKSANFWEVALLLGGCFDDVSLSTQTRDQLKTLLAELRAFQGVKALASTQTALVVFG
                     TSGWRDRIGEGMTVLNVHRVVRAIINTMQGDDYLQQQGYSSFDVVKKKGIVLMRDNRY
                     LGDVFLTAAKKELVQAQVKVHEAGECPTGVGSALVTELGAAGSINFTPSHNPMDWAGI
                     KFNPADGGPAGTNITTMIDAEAAKIMEDKEFAPAEPSTDQITTFCNAVDAKKMYREFI
                     LKSKNFDLLAIQNWLLAHKGELLIVVDYFHGSARGFVESLLGEELVAELQQSQAIEFW
                     HTNDDVSFGGKKPEPSAQNQRPLMARLKGSGRRFTLGVFMDPDADRIRLCDASLDIEV
                     NLFGPIMYANMLATGQKGGVVSTVASSDFALTIARENQQEVFETAVGFKWFREHFKSG
                     RAIIGFEESDGISFGDTLEKDALAGLLGALMIWVRSGENLSTQYERLQKRYGYYYPQK
                     TGIDLKGITPAQWLTYQKKVLGVLLGGLLAEGQKITVGESEKTIIQIMTDDGLKLVFD
                     DHSWILMRPSGTEPKFRIYYEITSAKALSGAQLKVLMDSYCQAGQKILQKARDHVAL"
     gene            2207..>4156
                     /locus_tag="VJC18_09710"
     CDS             2207..>4156
                     /locus_tag="VJC18_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45700.1"
                     /translation="MSTEAFSPESDTRSVVVRAPLAARLATPLPSQLSQTARQQLPDS
                     IPQPADPLERVAVFKTVPARYAAYQEILSSGLRTQADVNLALDLIALLHPTLAQLVEI
                     YRKAPVPVHFIKEAQSAYVERKSIDGPDRITSYAEGIFLGAGSELFVFIHELTHAIPF
                     AVLTEKSGWCDSPQHILDKRPYTWVYFPDDPNLKYRSTSSMCWVRVVRPESLFSPTSL
                     FNSIHRKYATLKYDRRVTPPTLSDLFTRLVGNYCCDEAASALAEVLLLEVLREQGFIV
                     WACQELEAGLEIIKQQRAIRAEPNMAFKAFYSNDFYLGDNHFLFYNARALYFVAEQVM
                     KDPDRLKEFSWSSRIDLTGFNLGDQNVALDIMRQIDPQEVLRVLKETISRPLSEGDSL
                     LPMQIRLRRYLAAMGVSRFLFDGPDRESRTEPHLSLAIDFGTKRPTPIKGCDDTRIDL
                     QAMQTVQRVTLSGAFVDFLNRLLAYSDREIVMNLEAYARLAELCDPALSPKLYRQLYA
                     LLFSNRHEQQTAIVFALSCLRSGYEDIGKTILLRTLCEAKVITEGIDLKAVTKAMVGP
                     IVGWEERWNLAVVELVRQGHGDVARLLLMGVEKTCAAEGRNYLKEIKRIFEDEEADRI
                     SCLFRGFPELFRKRLTKLARAYAGST"
BASE COUNT         1053 a          938 c         1113 g         1052 t
ORIGIN      
        1 caagaaatgt ttcatgcagg acttaaatgt caaacgggag tatttgtcaa aacttccttg
       61 aagaggtgag tgtaaactaa tatcagaaaa aaccatgaac tacacacaaa cactacaaac
      121 caaaacagac gatttggcaa aacacatttc aaactgtgtg caagcgattt ccgatcaggc
      181 gtttcgcgaa tctcttgcac agcgattgat tgaaacactt catcacgcgg tcaaatcggc
      241 caatttttgg gaggtggctc tcctgttggg tggttgtttt gacgatgttt cattgtccac
      301 acaaactcgt gatcaactca aaacattatt ggctgagcta cgggcttttc aaggtgtgaa
      361 ggctctggca tcgacacaga ctgctctggt ggtgtttggc acgtctggct ggcgcgatcg
      421 cattggtgag ggcatgacgg tactgaatgt gcatcgggtg gtgcgggcca tcatcaacac
      481 gatgcagggt gatgattatc tgcaacagca ggggtattct tcatttgacg tggtgaaaaa
      541 gaaaggcatc gtgctcatgc gcgacaatcg ctatctgggc gatgtttttt tgacagccgc
      601 caagaaagag ctggtgcaag cgcaagtcaa ggtccatgag gctggtgaat gtcccacagg
      661 cgttggttcg gcgctagtga cggaacttgg tgccgcgggt tccatcaatt tcacaccctc
      721 gcataacccc atggattggg cgggtatcaa attcaacccg gccgatgggg gccctgctgg
      781 tacgaatatc acaacaatga ttgacgccga ggcggccaaa atcatggagg ataaagagtt
      841 tgcgcccgca gagccgtcga ctgatcaaat caccacattt tgtaatgccg ttgatgccaa
      901 aaaaatgtac cgtgaattta ttttaaagag caaaaatttt gaccttctcg ccattcaaaa
      961 ttggttgctg gcgcacaaag gtgagttgct gattgttgtg gattattttc atgggtcagc
     1021 gcgcggattt gtggaatcgc tattgggaga ggagcttgtt gccgaactgc aacagtcgca
     1081 ggcaattgaa ttttggcaca caaatgatga cgtgagtttt ggtggcaaaa aaccggaacc
     1141 atcagcccaa aatcagcgtc ccctgatggc aagactcaag ggaagcgggc gccgattcac
     1201 tctgggtgtg ttcatggatc ccgatgctga tcgcattcgc ttgtgcgatg ccagtctcga
     1261 tatcgaagtc aatctgtttg gtcccatcat gtacgccaac atgctggcga ctggtcaaaa
     1321 aggcggtgtg gtgtcgaccg tggcgtcgtc tgattttgcc ctcaccattg cgcgcgagaa
     1381 ccaacaagaa gtttttgaaa cggcagtcgg gttcaaatgg ttcagggagc attttaaatc
     1441 cggtcgtgcc atcattggtt ttgaggaatc agacgggatt tcgtttgggg acacgctcga
     1501 gaaagacgcg ttggccgggt tattgggggc cttgatgatt tgggtgcgtt cgggtgagaa
     1561 tctttctaca caatatgagc gtttgcaaaa gcgatatggc tattattacc cgcaaaagac
     1621 gggcattgat cttaaaggca tcacacctgc ccagtggttg acttatcaaa aaaaggtatt
     1681 gggtgtgttg ttgggcggtc tgttggctga ggggcaaaaa atcacggtgg gcgagagcga
     1741 aaaaacaatc attcaaatca tgacggacga cgggttaaag ctggtttttg atgatcacag
     1801 ttggatcctc atgcgcccgt cggggactga gcctaagttc aggatctatt acgagatcac
     1861 cagtgcgaag gcattgtcgg gtgcccaact caaggtcttg atggatagtt attgccaagc
     1921 gggccagaaa atcctgcaga aagcccgtga tcacgtggcg ctgtagttgg ctgttgttcc
     1981 cgggccaaat aaatgtcacg agttcgtgtc cttcaggaca cggaaaatat tatttgtaat
     2041 caacatatct tggatcctgc cgataacctc agtgatgagg attcccggtt tatccgcaac
     2101 cccccgtgcg caaataaatg ttttggtgcc cgcaagtcca ttggggacga gtgtattccc
     2161 gactcaaagc gtgcctcatg tgcaaaccca cagtgacggg gttggcatgt ccactgaagc
     2221 attttccccg gagagtgata cgaggagcgt ggttgtccgt gcgcctttgg cggcgcgctt
     2281 ggcgacccct ctacccagcc aattgagtca aactgccaga caacagcttc ccgacagcat
     2341 ccctcagccg gcagatcctt tagagcgggt ggccgttttt aaaacagtgc cagcaagata
     2401 tgccgcgtat caggaaatcc tttcaagtgg gttacgcaca caggcagatg tgaatctggc
     2461 ccttgacctg atagccttgt tacacccaac cttggcccaa ctggttgaga tttacaggaa
     2521 agctccggtg cctgttcatt tcattaaaga agcacagtct gcttacgtgg aacgtaaaag
     2581 cattgatggt ccagatcgca ttacaagtta cgcagagggt attttcctgg gagcgggcag
     2641 tgaactattt gtgtttattc atgagctcac acatgccatt ccctttgccg tcctcacgga
     2701 aaaatcgggg tggtgcgact caccccagca tatactcgat aaaaggccgt acacgtgggt
     2761 ttattttccg gatgatccca atctaaaata cagaagtacc agtagcatgt gctgggtcag
     2821 ggtggtacgg ccagaaagcc tgtttagccc aacgagtttg tttaacagta ttcaccgaaa
     2881 atacgccacg ttgaaatatg atagaagagt gacccctccg acgctctcag atctgttcac
     2941 aaggttggtg gggaattatt gttgtgatga agccgcgagt gcgctggcag aagttcttct
     3001 attggaggtc ctccgtgaac agggatttat cgtatgggca tgtcaggagt tggaagctgg
     3061 cctggagatc atcaaacaac agcgggccat aagggccgag ccgaatatgg cgttcaaggc
     3121 cttttattcc aacgatttct atctgggcga caaccacttt ctattctaca acgcacgcgc
     3181 tctctacttt gtggctgaac aagtcatgaa agaccccgac aggttgaaag aattttcctg
     3241 gtcgagccgt attgatctga caggttttaa tcttggcgat caaaatgtgg cgctggatat
     3301 catgcgtcaa atcgatcccc aggaggtgct tcgcgtactc aaggagacca tttcacgacc
     3361 gctatcagaa ggcgatagtc tgttacccat gcagattcga ttgagacgct atctcgccgc
     3421 catgggggtt tcacgttttt tgtttgatgg tccagacagg gagtctcgta cagagccaca
     3481 tttatccctc gcgattgatt ttgggacaaa acgccccaca ccgataaaag gttgtgatga
     3541 tacccggata gacctgcaag caatgcagac agtgcaacgt gtgacgcttt ctggtgcctt
     3601 tgtggatttt ctcaatcgtc tgctggccta ttcagacagg gaaatcgtca tgaatctgga
     3661 ggcgtatgcc aggctggctg aattgtgcga ccctgctctg tcgcctaaac tgtatcgcca
     3721 attgtacgcc ctcctgttca gtaatcgtca tgaacaacag acagccattg tgtttgccct
     3781 gtcatgcctt cgctcaggtt atgaagatat cggtaaaaca atccttttaa gaaccttgtg
     3841 cgaagcgaag gtcatcaccg aagggatcga tctcaaagca gtaaccaaag ccatggtcgg
     3901 acccattgtg ggctgggagg aacgttggaa tctggctgtt gttgagttgg tcaggcaggg
     3961 acatggcgat gtggccagac ttctgctgat gggcgttgaa aaaacttgtg cagcagaggg
     4021 tagaaattat ttaaaggaaa taaaaagaat ttttgaggat gaggaagcag accgaatatc
     4081 atgtctcttt cgtggatttc cggaattgtt ccggaagaga ttgaccaaac tggctcgtgc
     4141 ctatgcagga agcacg
//
LOCUS       DATDUN010000699         1337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_37510, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000699 DATDUN010000000
VERSION     DATDUN010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37510"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1337)
                     /locus_tag="VJC18_09715"
     CDS             complement(<1..>1337)
                     /locus_tag="VJC18_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLD45701.1"
                     /translation="LVATDAAGEGVNLQNANLMVNYDMPWNPNRLEQRFGRIHRIGQT
                     EICHLWNLIANETREGEVFQRLFEKLEEARKALGGKVFNVLGDDIFSEVPLRDLLIRA
                     IRYGEDPAVRNQLFVKVDGALDIEHIKSIMTRNALCEDVMTADRVFAIKTEMEKAEAR
                     KLQPFFIKSFFNKAFEHFKGSLRQREPGRFEITHVPAIVRDRDRQITGRDRRNLSPVL
                     KRYERVCFEKQYVRLIDRVNTPMASLIHPGHPLVQSLVDLVLEEHRTKLKQGTVLVNA
                     QDMGVEPRVLFLLDHAVRESGENGRSASRRIQFVEINERGEAINAGYAPHLDYEALPV
                     SDYSLVQDILQSHWMTDDLERVALAYASQHIVPEHYKEVKERRERQADKTLAAVNERL
                     INQINFWSDRHIKLKEDLDAGKDVRLNLDNARRTVDELTVRLASRKKELELMR"
BASE COUNT          339 a          331 c          287 g          380 t
ORIGIN      
        1 ttgcgcatga gttctaattc tttttttcga gacgccaacc tgacagtgag ttcatcaacc
       61 gtacgtctgg cattatcgag attcagccgc acatctttgc ctgcatccaa atcttccttg
      121 agcttaatgt gcctgtctga ccaaaaattt atttgattga tgaggcgttc gttgacggcc
      181 gccaaggttt tgtcggcctg tcgttcccgc cgctctttga cctctttata atgttcggga
      241 acgatatgtt gtgatgcgta ggctagggcc acacgttcaa gatcgtctgt catccaatgt
      301 gactgtaaaa tatcctgcac caaagaataa tcactgactg gcagggcttc ataatccaaa
      361 tgtggtgcgt agccagcatt tatagcctcg ccgcgctcgt tgatctccac aaattgaatc
      421 ctgcgtgacg cgcttcgtcc gttttcacca ctctctctga ctgcatgatc tagcaaaaat
      481 aagactcgtg gttctactcc catatcctgt gcgttgacca agactgttcc ttgtttaagt
      541 tttgtacgat gttcctcaag cacaagatca acaagtgact gcactaatgg atggccggga
      601 tgaatcaggc tggccattgg ggtattgacc ctgtctatga gcctcacata ctgtttttca
      661 aaacagactc gttcatatct ttttaataca gggcttaagt tgcgccgatc acgacctgta
      721 atctgtcgat cgcggtcacg aacaatggca ggtacatgtg tgatttcgaa gcggcctggt
      781 tcgcgctggc ggagagagcc cttaaaatgt tcaaaggcct tgttaaaaaa tgatttgata
      841 aaaaatggtt ggagttttcg cgcttcagct ttttccatct cggtcttaat ggcaaaaacc
      901 cgatctgctg tcatcacatc ctcgcaaaga gcattgcgag tcataatgct tttgatgtgt
      961 tcaatgtcta gggcgccatc aacctttaca aacaactgat tacgaaccgc aggatcttcg
     1021 ccatagcgaa tggccctaat caacaaatca cgcaaaggca cttcggaaaa tatatcatcg
     1081 cccaatacat taaagacttt gccaccaagg gccttgcgcg cttcttcgag tttttcaaac
     1141 aacctctgaa aaacttcacc ctcgcgagtt tcattggcta tgagattcca aagatgacag
     1201 atttcggtct gaccaatgcg atgaatacga ccaaagcgct gctccaaacg atttgggttc
     1261 caaggcatgt cataattaac catcaggtta gcgttttgca ggttaacacc ttcgcctgcg
     1321 gcatctgtag caaccag
//
LOCUS       DATDUN010000700         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_288180, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000700 DATDUN010000000
VERSION     DATDUN010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288180"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VJC18_09720"
     CDS             complement(<1..445)
                     /locus_tag="VJC18_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD45702.1"
                     /translation="MSTTISSGLRQLERKQPKGLYILFFTEMWERFSYYGMRAILVLY
                     LVSETSGGPLGGLGWTKSDALALYGWYTMLVYLASIPGGLIADRITGQRKAVMYGGLL
                     LCVGHGVMALPGIFAFYSAITLIICGVGMLKPNISTLVGSLYAPGD"
     gene            complement(741..>2412)
                     /locus_tag="VJC18_09725"
     CDS             complement(741..>2412)
                     /locus_tag="VJC18_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HLD45703.1"
                     /translation="SLRDLSEMTYHEEALVLVLAHSQNSQRLIDLIKQRFHEETQPVI
                     QNATAMLQLDDATKIPFVIDHDLWSYCFAYARHIQVILQRIEPDQGLLTEEEKAAFHD
                     IFHQCGLLLQRANTPETPAQLQDFVINLEKLLPLLGTETLEDVRKIYLNSLEARTVDH
                     VSDTVESESPRDIENMVSLARLYQDRLRSYRSLPEHGLNLVYGPLCDVARKGTPGYME
                     KVGRVFAHDPVVTNLLAALTIEATHQDYPQMGFVIKHAPGIGAIDMNPEWEGLVADYR
                     DKAAILRELSAFTATLRGHSQVAVMVTGAAYPNLESETATLRPACLSPDVCSLLRANF
                     GEDVVLFSDDLGTDAIKSYYGISNFTADDCAKSIADATLAGVDVCLFYSHSKDMLFTV
                     LDELEKTTEAGQVSREVLEASVLRILRYKQILFPDNEVLRDPEQLVKRMSIKELWAQK
                     IIYPCYCVADSKSYIGMGVGGLTFEGVKNLEYYTQMTRELLRESAATIPPFMANNSPQ
                     ETAGSRSPMNPVRQEEIDLLAELATQLETAYPDGVPQEVAEQILSSLR"
BASE COUNT          642 a          603 c          528 g          639 t
ORIGIN      
        1 gatcccccgg ggcataaaga ctgccaacca aggtagagat atttggtttg agcatgccca
       61 caccacaaat gatcagcgtg atcgccgagt aaaatgcaaa gatcccaggc aacgccatga
      121 caccgtgacc aacacaaagc aagagtccac catacatgac ggccttgcgc tgacccgtga
      181 ttcggtcggc tatgaggccg cccggaatcg aagccagata caccaacatc gtataccacc
      241 cgtacaatgc cagagcgtcg gatttggtcc accccaaacc acccagaggc ccaccagatg
      301 tttctgatac cagatacaaa accagaatgg cccgcattcc gtagtagctg aacctctccc
      361 acatctctgt gaaaaacaga atgtaaagac ccttcggttg cttgcgttca agctgtcgca
      421 gaccggacga aatggttgtt gacatgtgtt gctccttttt ccgaatgttt aaacacaaat
      481 cattcctatc gttcatgtcg ctgtcaattg taaaggtgct ctcaacgcat aaaaaaaata
      541 ccgtgtcctt tgggacacgg tgtcttgcac aataatgatt tgtttggtcg caatcccgat
      601 aaggcttgtc tacatagata aggggtcaga taaggggtca aagattgaca agagacaagt
      661 tcataaagaa gattgtccct agggcaactt gtctcttgtc aatctttgac ccctttttgt
      721 tgtgacccct ttttgttgaa tcagcgcaac gaacttaaga tctgttctgc cacttcctgt
      781 ggaaccccat caggataggc tgtttcgagt tgagtcgcca actctgcaag aagatcgatt
      841 tcttcctgcc gcacagggtt catcgggctt ctgctccctg ccgtttcttg gggagaattg
      901 ttggccatga agggcgggat cgtggcagcg ctttctcgca acagttcacg agtcatttgc
      961 gtgtaatatt ccagattttt tacaccctca aaagtcaacc caccaacccc cattccaatg
     1021 taggacttcg agtcagcaac gcagtaacag gggtaaatga ttttctgagc ccagagttcc
     1081 ttaatgctca ttcgcttgac tagctgttca ggatctctca agacttcgtt gtccggaaag
     1141 agaatttgtt tgtaacgcaa tattctcaaa acagaagctt ccaatacctc tcgggaaacc
     1201 tgacctgctt ccgttgtttt ttccaattca tccagtaccg tgaaaagcat gtcttttgaa
     1261 tgcgaataaa acaagcagac atctacccca gccagcgtag catcggcaat cgacttggcg
     1321 cagtcgtctg ctgtgaagtt gctgataccg taatatgatt taatggcatc cgtacccaaa
     1381 tcgtccgaaa acaacaccac atcttctcca aaatttgctc ggagtaaaga gcacacatcg
     1441 ggtgacaaac aggctggtct caaggtggct gtttcggatt ccagattggg atacgccgct
     1501 ccggtaacca taaccgcaac ttgtgaatgc cctctgagcg tggcagtgaa agcagagagt
     1561 tcacgtaata tcgccgcctt gtccctgtaa tccgccacca ggccttccca ttcggggttc
     1621 atgtctattg ccccaatgcc tggggcatgc ttaatcacaa atcccatttg gggatagtct
     1681 tgatgtgtgg cttcaatagt cagcgcggca agcaaattgg tcacaacagg gtcatgcgca
     1741 aagacccgtc ccaccttttc catatagcca ggtgttcctt ttctggccac atcacaaaga
     1801 ggaccataaa caagattgag accatgttct ggcagactgc gataacttct taatctatcc
     1861 tggtacagcc gtgccaaact taccatgttt tcgatgtctc tggggctttc gctttcaaca
     1921 gtatccgaaa catgatcaac ggtccttgcc tcaagactgt tcagataaat tttccgtaca
     1981 tcctcgaggg tttctgtccc caacaagggc aaaagttttt caagattaat tacaaaatct
     2041 tgtaactgtg cgggagtttc tggtgtattc gctctttgaa gcaacaaacc acattgatga
     2101 aagatgtcat gaaatgctgc tttttcctct tctgtcaaaa gaccttgatc aggttcaatt
     2161 cgttgcaaga tcacctgtat gtgtctggca taggcaaaac aataactcca caaatcatga
     2221 tcaatgacaa aaggaatttt cgtggcatca tcgagttgca acatcgctgt ggcgttttga
     2281 atgactggtt gtgtctcttc gtggaaacgt tgtttgatca gatctatgag cctttgggaa
     2341 ttttgagaat gggcaagaac cagaaccaag gcttcttcat gataagtcat ctccgacagg
     2401 tctcgaagtg at
//
LOCUS       DATDUN010000701         2923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_368678, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000701 DATDUN010000000
VERSION     DATDUN010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368678"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VJC18_09730"
     CDS             complement(<1..226)
                     /locus_tag="VJC18_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLD45704.1"
                     /translation="MTLSVQFLGATGTVTGSRYLLTNNNKRVLIDCGMFQGLKDLRLR
                     NWTPFPVDPKTLDAILITHAHLDHSGYLPRL"
     gene            complement(344..766)
                     /locus_tag="VJC18_09735"
     CDS             complement(344..766)
                     /locus_tag="VJC18_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLD45705.1"
                     /translation="MNNKGFTLIELILVIAILGIMAVSAMPKIFSLTNEANEAVMNGV
                     LGQLQAAVAIWEAKQMATTGSVPLVAQFDALPNNTLCSVVNRCFSDFLKDPITQSGWR
                     KKDKFQYLYDKVSSGKLLVFMQAQSKWTRQNPPPPPPP"
     gene            complement(935..1918)
                     /locus_tag="VJC18_09740"
     CDS             complement(935..1918)
                     /locus_tag="VJC18_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45706.1"
                     /translation="MKPKLFLEDVGKISTSVAGKNIWLVEDLHRHELFKGRHLPNKIY
                     VVDFPGRGMGQEFLLSRHICGPLKFLMIQQIGHSMIRLFKNAFVGRVAQFVILWGGRP
                     LDLLAADPPLYEDNLIDTTYIKLTRVEDASVNRGWRVVKSSVVGEFWKDDTWLIMEEC
                     IASGKTLKYFVDESFGEHRPKKLFIFPVCASLEGLEGINEVCVKHGVEFIPVLNSAIV
                     QVAEKGVCKPFTDLGLQPLTIVTRNFYKTLSERYQGSPLCWVGDIGDSLYKTHEYMIE
                     TLTDMFKLNMDFGKEDFSQWNHALFSKTFQTKLKKRESEVYEKLKSVMGDG"
     gene            complement(1957..>2923)
                     /locus_tag="VJC18_09745"
     CDS             complement(1957..>2923)
                     /locus_tag="VJC18_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45707.1"
                     /translation="KHTAIAHVTPEIWSQIPYLKDNNGALPPFVLAVGDARRLQIACE
                     TLPLKNIVSLHSEVEKLVGLKGRGRVDLILATYEHEHKSVPLLLVETQMGMPATEINL
                     REVLAHCSQEYQIGDQKLKTNGLFIIRAGTAGGINTIDDTQPKLVVGDLINAQFSIGW
                     SGALIESLAGLNFASPEVLFNFQKNWKKAGHSFTNDNKYPLGKSSDSVVTAISGSAKE
                     LNLRCFEGGNFSKDSLYAEMNDEAFIKLRKRYNVMSTEMEQIIVCKLMSDFALHNIIL
                     KTGMVSGVIGTIPGASFAKEYDEVEKNVLRVAARALWKLGTQGDS"
BASE COUNT          723 a          730 c          715 g          755 t
ORIGIN      
        1 ccaatcgtgg cagataacca gagtggtcca aatgcgcgtg cgtgatcaaa atggcatcga
       61 gggttttggg atcgacggga aacggggtcc agttgcgcaa gcggaggtcc ttaagcccct
      121 gaaacatccc gcagtcgatc aacacgcgtt tgttattatt tgtgagcaga taacgagaac
      181 ccgtcacggt gcctgtggca ccgaggaatt gcacagataa tgtcatggtg tcttatgaca
      241 tgaaaatgat gtgagagcaa atgataggtt tacttaggat cgtcattgct cctgccggcc
      301 gacaggagca atgacgaact tgtttaccca tgagagatca cgatcaggga ggtggaggag
      361 gtggtggatt ttgtcgagtc catttgctct gggcctgcat gaatacgagc aacttcccag
      421 atgagacttt gtcgtagaga tactggaact tgtctttttt cctccaaccg ctttgtgtta
      481 tgggatcctt tagaaaatcc gaaaaacagc gattgactac tgagcacagg gtattatttg
      541 gcagggcgtc gaattgagca accaacggca cagagcctgt tgtggccatt tgttttgcct
      601 cccaaatggc taccgccgcc tgtaattgac caagaacgcc gttcatcacc gcttcattgg
      661 cctcgtttgt cagcgaaaag atcttgggca tggctgagac agccataata cccaaaatcg
      721 caatcaccaa gatcagttcg atcaaggtaa aacctttgtt attcatgtta tccccctttt
      781 gactaaatta aaggtgaccc catcacccag aacctagtta acccctatta tttacaatca
      841 accctgccta cgcaatgggg aaaaacacta tttttaaggg taatgagtaa tgagtaatga
      901 gtaatgagac caaagactta acagccaaaa cccatcaccc atcacccatc acagatttca
      961 atttctcata aacctcggac tcccgttttt tcagttttgt ttgaaatgtt ttcgaaaaca
     1021 aggcgtgatt ccattgagaa aagtcctctt tgccaaaatc catgttgagc ttgaacatgt
     1081 cggtcagtgt ttcgatcatg tattcgtgag ttttgtagag tgaatcccca atgtccccca
     1141 cccagcacag tggtgatccc tgatagcgct cactcagtgt tttgtaaaaa tttcgcgtga
     1201 caattgtgag cggttgcagg cccagatcgg taaagggttt gcagacccct ttttcggcca
     1261 cctgaacaat ggcgctattg agcacgggaa tgaattccac gccgtgtttg acgcaaacct
     1321 cgttgatgcc ctccaaccct tccagactgg cacagacggg aaagatgaaa agttttttgg
     1381 gacggtgttc cccgaacgat tcatccacaa aatatttgag cgttttgccc gaggcgatgc
     1441 actcttccat gatgagccag gtgtcatcct tccaaaactc acccaccacc gaactcttga
     1501 ccacgcgcca accgcgattc acgctcgcat cttcgacgcg cgtgagtttg atgtacgtgg
     1561 tgtcgatgag gttgtcttcg tacagaggcg gatccgcggc caaaaggtct agtggtctgc
     1621 ccccccataa aatcacaaac tgggcgacgc gtcccacaaa agcgtttttg aacagccgga
     1681 tcatgctgtg gccgatctgc tggatcatca ggaatttgag tggaccgcag atgtggcgac
     1741 tgagcaaaaa ttcctgaccc atgccgcgac ccggaaaatc caccacatag attttattgg
     1801 gaagatggcg ccccttgaac agttcatgac ggtgcaggtc ttcgaccagc cagatgttct
     1861 tgccagccac cgaagtgctg atctttccaa cgtcttctaa gaataacttt ggtttcataa
     1921 tatcacgtgt tcacgtgttc atgttgtagg ggcgactcac gagtcgccct gtgtccccag
     1981 tttccacaag gcacgagcgg caacgcggag cacatttttt tcgacctcat cgtactcctt
     2041 ggcgaacgaa gcccccggga tggtcccgat cacgcccgag accatgcctg tcttgagtat
     2101 gatgttgtgg agtgcaaagt cgctcatgag tttacagaca atgatttgtt ccatctcggt
     2161 actcattaca ttgtagcgtt tacgcagttt gatgaaggcc tcgtcgttca tttcggcata
     2221 cagggaatcc ttgctgaaat tgccaccctc aaaacaacgc aggttcagtt ctttggcaga
     2281 accgctgatg gccgtcacca cagaatcaga ggattttccc aacgggtatt tgttgtcgtt
     2341 ggtaaaagaa tggcccgctt tcttccagtt tttttgaaaa ttaaacaaca cctctggtga
     2401 ggcaaaattc aatcccgcga gcgattctat gagcgcgccc gaccaaccaa tgctgaattg
     2461 ggcgttgatc agatcgccca caacaagttt gggctgtgtg tcatcgatcg tgttgatgcc
     2521 tccggccgtg cccgcgcgga tgatgaacag accattggtt ttgagctttt gatcgccaat
     2581 ttggtattcc tgcgaacaat gggccaggac ctcacgtaga ttgatctcag ttgcgggcat
     2641 gcccatctga gtttcgacca gcaagagggg aacggatttg tgctcgtgtt catacgttgc
     2701 cagaatcaga tcgacccgac cgcgtccttt gagccccacc agcttttcca cttcactgtg
     2761 aagtgacaca atatttttga gtggcaaggt ttcgcacgca atctgcaaac gtctggcatc
     2821 gcccactgcc agcacaaaag ggggcagggc gccgttgttg tctttgaggt acggaatttg
     2881 tgaccaaatt tctggggtga catgggcaat ggccgtgtgt ttg
//
LOCUS       DATDUN010000702         1214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_288267, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000702 DATDUN010000000
VERSION     DATDUN010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288267"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1214)
                     /gene="carB"
                     /locus_tag="VJC18_09750"
     CDS             complement(<1..>1214)
                     /gene="carB"
                     /locus_tag="VJC18_09750"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878769.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HLD45708.1"
                     /translation="GTQMKSVGEVMGIGRTFKESLQKAMRSLEIGKSGFDEVKIVAKK
                     KNERLAEIAQNLITPVPDRLWWIGEAFRYGFRVKEIHDHTKIDPWFLEQIREIIDYEE
                     RLRGQRLRDISQKILREAKRLGFSDMRLARLFDTTEDQVRQKREKTKMRPVFKTVDTC
                     AAEFTSFTPYYYSTYETENEVVSSKKKKVVILGGGPNRIGQGIEFDYCCCHACASLRE
                     EGYEVVMVNCNPETVSTDYDTSDRLYFEPLTKEDVIRILQEEKPIGIIVQFGGQTPLK
                     LSKPLEKWGARILGTSSNSIDRVEDRKRFSAFIKKLRFRQPENGIATSLPEAIKAARR
                     IGYPVLVRPSYVLGGRAMEIVYDEKSLCEYMHEAVRASEERPVLVDRFLENAIEVDVD
                     ALCDGKVALIGG"
BASE COUNT          287 a          361 c          244 g          322 t
ORIGIN      
        1 cccgccgatc agagccactt tgccgtcgca caaggcatcc acatcgacct caatcgcgtt
       61 ctctaaaaaa cgatccacta aaaccggccg ctcctctgac gcacgcacag cctcgtgcat
      121 gtattcacat aaactctttt catcatatac aatttccatg gcacgcccac ccagaacgta
      181 cgagggccgc accaaaacgg ggtacccaat ccggcgagcg gccttgatcg cctctggcaa
      241 gctcgtcgcg atcccattct cgggttgtct gaacctgagc tttttgatga aggccgagaa
      301 tcgtttgcga tcctccacac gatcgatgct gttggacgac gttcccagaa ttctcgcccc
      361 ccatttttcg agaggcttgg agagtttgag cggggtctgg ccgccaaact gaacgatgat
      421 ccctattggt ttttcttcct gcaaaatccg gatcacatct tccttggtca agggctcaaa
      481 ataaaggcga tccgaggtgt cgtaatccgt gctcactgtc tcgggattac agttcaccat
      541 gaccacttcg tacccttcct cacgcaatga agcacacgca tgacagcaac aataatcaaa
      601 ttctattccc tgaccaatcc tgttggggcc gccacccaaa atcacgactt tctttttctt
      661 cgaagacaca acttcatttt ctgtttcgta ggtagaataa taatacggcg taaaggatgt
      721 gaactctgcg gcacaggtgt ccaccgtttt aaaaacaggc ctcatcttgg tcttttcgcg
      781 cttttgtctg acctgatctt cggtcgtatc aaagagtctc gccaatctca tgtctgaaaa
      841 acccaatcgt tttgcctctc tcagaatttt ttgggagata tcccgcaacc gctgaccccg
      901 cagacgttcc tcataatcga tgatttcgcg tatttgttcc aaaaaccacg ggtcaatttt
      961 ggtatgatca tgaatttcct tgacgcgaaa accataccga aacgcctcac cgatccacca
     1021 caacctgtcg ggcaccggcg tgatcagatt ctgggcgatt tccgcaagtc tctcgttttt
     1081 tttcttggcc acaattttga cctcgtcaaa accggatttg ccaatttcca gggaacgcat
     1141 tgccttttgc agactctcct taaacgttct gccaatcccc atgacctcac ccaccgattt
     1201 catctgtgtg ccca
//
LOCUS       DATDUN010000703         3188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_321449, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000703 DATDUN010000000
VERSION     DATDUN010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321449"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /gene="gatD"
                     /locus_tag="VJC18_09755"
     CDS             <1..1384
                     /gene="gatD"
                     /locus_tag="VJC18_09755"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HLD45709.1"
                     /translation="TSDAFKGYKGKALDALKQHGVRVWSDAEVKTSRGDFRGIILPRS
                     ESDDDEHIVLKLNTGYNVGVAVATITSMTEEGYKEAHYKIPEKEFPVSPNKPNVKLIG
                     TGGTIASRLDYRTGAVIPAFSPGELYGAVPELADICNLSTEKLFAVFSENMGPEQYKK
                     LAIRIGQEIEKGVDGILIGHGTDTMHHTATALSYMVQDSPVPIVMVGSQRSSDRPSSD
                     AALNLIHGTKAASSADIAEVMVCMFGPTSNEYGLLHRGTRVRKMHSSYRSTFRTIGDV
                     PLAMINRQKITPLTDDYKRRRKDRNVKITPTFEEKVSIVYYYPNMQPDMIDSLTDHGY
                     KGIIIAGTGLGHVNKPLYPAIERAVAKGVHIYMTVQTLWGYVHMFVYDTGRDLMAKGV
                     VPLDNMLPEVAYIKLGWALGQTSDHDEVVKIMKTPISHDITEREPCNGYLVFQGGIPE
                     VEAFLRTVHK"
     gene            1758..2105
                     /locus_tag="VJC18_09760"
     CDS             1758..2105
                     /locus_tag="VJC18_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45710.1"
                     /translation="MLAKNLVIGSIPAKTITAIKIGKNKIYKSPIGTLVYFGIAPHLF
                     FGFETQKGISFANKEKAFLDTLYFYQRGHKFSFNIFQDINIKSLDVKTIQRYLKFYKN
                     PKVVKFVKGMIND"
     gene            2098..2838
                     /locus_tag="VJC18_09765"
     CDS             2098..2838
                     /locus_tag="VJC18_09765"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLD45711.1"
                     /translation="MIEINSAEALMVWVMNRFSETLGEHAILKGGMVLRLLDCPRYTN
                     DLDYIFVPFDSKKKIWPMIERTLQELDGAQIQHEMNSKCLRIRVTFRNYSIQIESNVM
                     LECKTQPMSTSAMAKRHNQLPRVIRVMRFDWALANKLAAWNERELIRDLYDAHFLSGI
                     MNEKPDLDILIERLQKINYTGKTRHKNLPKSLTLNEFVERLKTAVKNLTAKEVEQELR
                     DYLAPEELAGLDKRMTVGLMRLADNLLK"
     gene            2931..3092
                     /locus_tag="VJC18_09770"
     CDS             2931..3092
                     /locus_tag="VJC18_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45712.1"
                     /translation="MNFITAKEAFEKTKTLTNAVLLDVRTPKEFQPFFILCRGIKVKN
                     LVRLIKVNT"
BASE COUNT          952 a          718 c          742 g          776 t
ORIGIN      
        1 gacatctgat gcattcaaag ggtacaaggg caaggctctg gacgccctca aacaacacgg
       61 tgtgcgcgtc tggagtgatg ccgaggtcaa aacatcacgc ggtgatttca gggggatcat
      121 tttgccccgc agcgagagtg atgatgacga gcacatcgtg ctcaaactca atacgggcta
      181 caatgtgggc gtggcggttg ctacgatcac atccatgacg gaagagggtt acaaggaagc
      241 acactataaa atccccgaaa aagagtttcc tgtttctccc aacaagccca atgtgaagct
      301 cattggcacc ggcgggacta ttgcgtcacg actggattac cgcacggggg cagtgattcc
      361 cgcgttttcg ccgggagaac tttatggcgc ggtccctgaa cttgccgaca tatgcaattt
      421 gagcaccgaa aaactttttg ccgtgttttc cgaaaacatg gggcccgaac agtacaaaaa
      481 actggccatc cggattggtc aggagattga aaaaggtgtg gacggcatcc tcattggcca
      541 tgggacagac accatgcacc acacagccac ggcattatcg tatatggtgc aggattctcc
      601 cgtgccgata gtgatggtgg gatcacagcg ttcatctgat cgtccttcat ctgatgccgc
      661 gctgaacctg attcacggga ccaaggccgc cagttctgcc gacattgccg aggtgatggt
      721 gtgcatgttt ggccccacat ccaacgaata tggccttctg caccgtggca cacgcgtgcg
      781 caagatgcac tcatcgtatc gttccacatt ccgcacgatt ggtgacgtgc ctctggccat
      841 gatcaacagg caaaaaatca caccgctcac ggatgattac aaaagacgcc gcaaagacag
      901 aaatgtgaaa atcacgccca cgtttgagga aaaagtctca atcgtttatt attatcccaa
      961 catgcaaccc gacatgatcg attccctcac cgatcacggt tacaagggga tcatcattgc
     1021 gggcacgggg ctggggcatg tcaacaaacc cttgtacccg gccattgagc gggcggtggc
     1081 caagggcgtg cacatttata tgaccgtgca gacgctgtgg ggttatgtgc acatgtttgt
     1141 gtacgataca ggcagagacc tcatggccaa aggggttgtg ccgcttgata acatgttgcc
     1201 cgaagtggcc tacatcaagc tcggttgggc tttgggacag acgagcgatc atgacgaagt
     1261 ggtcaagatc atgaaaacgc ccatttcgca cgacatcacc gagcgcgaac cctgcaatgg
     1321 gtacctggtt tttcagggcg gcattcccga ggtggaggcg tttttgagaa cagtacacaa
     1381 gtaggggcag gttccaaacc tgaccaataa aaaacagaca caaggcgaag acgtggagcc
     1441 ttgtgacagg ttaacaaccc attttcatat agacagataa tatacgattt gatataatat
     1501 ttgtcgatat gaaatatttg gatacatacc aacgaattat cggatattct gctgtgttac
     1561 agggcgtgtt ttcggttttt gatatcaaaa atctactgcg agaaagcaag cctgccctca
     1621 tttacaaaca catagcacag ttggaaaaac aaaaaatcct cacgcgtttc acacgaggat
     1681 attatgtcac ccctgatttc agtcttgaag tgttatctca acgggtttgc cctcaatcgt
     1741 acatcagttg cggcaacgtg ttggcaaaaa atttagtgat cggttcaata ccagccaaaa
     1801 caataacagc gattaagatc gggaaaaata aaatttataa aagtcctatt ggcacacttg
     1861 tgtattttgg gatcgctcct catttgtttt ttgggttcga aacacaaaag ggaatatctt
     1921 tcgccaataa agaaaaagca ttcttggata cattgtattt ttatcaacga ggtcacaaat
     1981 tttcttttaa tatttttcag gacatcaaca tcaaatctct cgatgtaaaa acaatacaaa
     2041 gatatctgaa attctataag aatccaaagg ttgtgaaatt tgtgaaggga atgatcaatg
     2101 attgaaatca attctgcaga agccctgatg gtgtgggtca tgaatcgttt ttcagaaact
     2161 ctgggcgaac atgccatcct caaaggcggc atggtgttgc gacttcttga ttgtccacga
     2221 tacacaaacg atctggacta catttttgtg cccttcgatt caaaaaagaa aatttggccc
     2281 atgattgagc gaacacttca agagctcgac ggcgctcaaa ttcaacacga aatgaactca
     2341 aaatgtttga ggattagggt gacctttcgt aattattcta ttcaaattga gtccaatgta
     2401 atgctggagt gcaaaaccca accgatgagt acaagtgcga tggccaaacg acacaatcaa
     2461 ctgccacgtg tgattcgggt catgcgcttt gattgggcct tagccaacaa gttggccgcc
     2521 tggaatgaac gtgaactgat ccgtgatctt tacgatgctc attttctatc tggcattatg
     2581 aacgaaaaac cagatctcga tattttgatc gagcgcctgc aaaaaatcaa ttatacaggg
     2641 aaaacccgtc acaagaattt gccaaaatct ttgacactca atgaatttgt cgaaagacta
     2701 aaaacagctg tgaagaatct tacggcaaaa gaagttgaac aggaactccg ggattatctt
     2761 gcaccagaag aactagcagg acttgataaa agaatgacgg tagggttaat gcgtttggcg
     2821 gacaatttgt tgaagtgaat ttcacatcac catcgccccg atactgcaca aacacaggtt
     2881 gacaatcatc aatttagaca tagatacaca ctaataaaac aggagaaccc atgaatttca
     2941 ttaccgccaa agaagctttt gaaaaaacca aaaccttgac caatgccgta ttgctggatg
     3001 tgcgcacacc aaaagagttt caaccctttt ttattttatg ccgtggaatc aaagtaaaaa
     3061 atcttgtcag actcattaag gtgaacacat gaaaaaaaca tatgtcatcg tcggcggcgt
     3121 ggcgggaggt gctacagcgg ccgccaaact caggcgcgaa gatgaacaag ccgagatcat
     3181 catctttg
//
LOCUS       DATDUN010000704         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_494346, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000704 DATDUN010000000
VERSION     DATDUN010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494346"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VJC18_09775"
     CDS             complement(<1..480)
                     /locus_tag="VJC18_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLD45713.1"
                     /translation="MRITQKGSEIIAKSLEREGVTHVFGYPGGASLDINQALMSSRKI
                     KTVLTRHENSAVFAASGFARATGRVGVCMAASGAGATNLITGIMDAKKDSIPLVAITG
                     QVPTHVIGLDAFQESNIFSATLPCVKHSYLVKDTKDIPSIVQEAFYIANTGRKGPVLI
                     "
     gene            complement(500..>2299)
                     /gene="uvrB"
                     /locus_tag="VJC18_09780"
     CDS             complement(500..>2299)
                     /gene="uvrB"
                     /locus_tag="VJC18_09780"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HLD45714.1"
                     /translation="KTLAAQLYSEFKSFFPENAVEYFVSYYDYFQPEAYIPRNDLYIE
                     KDSSINDDIDKLRHAATHSLLTRSDVIIVASVSCIYGLGSPEAYQGLLIDVRKGMNIS
                     RSEVINKLVTLRYERNDIDFHRGVFRSRGDVLDIFPAYEEDRAIRVEFFDDEIESLKI
                     IDSLRGEVIDKIDRIAIYPGSHYVLPEEKKMQAIASIRLELEQRLESFDQQGKLVEKQ
                     RLQQRTQYDLEMIEEVGFCKGIENYSRHFDQRLPGSPPPTLLQYFPRDFLLFVDESHI
                     SVPQIGGMYHGDRARKQTLIEYGFRLPSALDNRPLTFEEFEACLDQAVFVSATPGSYE
                     LQQTPGQVIQQVIRPTGLVDPQVQVKPVNNQVDDLMAAIKETVNTGNRVLVTTLTKRQ
                     AENLSKYFSEVGIRCRYLHSDIDTIERFQILRDLRLGDFDVLIGINLLREGLDLPEVA
                     LVAILDGDKEGFLRSERSLIQTFGRASRNINGKVIVYADTVTGSLKRALDETQRRREK
                     QLAYNRENSITPTTVSKAIRDGLPHEIKADYIDIPKALQGDQVMNVRDIPGLILKLKK
                     QMQICAQKMDYEEAAQLRDQIKHLREVELTLGG"
BASE COUNT          538 a          619 c          561 g          581 t
ORIGIN      
        1 gatcagcact ggccctttgc gcccggtatt ggcaatataa aaggcctcct gcacaatgga
       61 gggaatgtct ttggtgtcct tgacaaggta gctgtgtttc acacaaggca aagtcgcgct
      121 gaaaatattg ctctcctgaa aagcatcgag accaatcaca tgtgtgggaa cctgtcctgt
      181 aatggccacg agcgggattg aatccttttt ggcatccatg atgcccgtga tgagattcgt
      241 cgcgccagca cccgatgcgg ccatgcacac acccacgcga cctgtggccc gagcaaaacc
      301 gctggccgcg aagacagccg aattttcgtg acgggttaaa actgttttga ttttcctgga
      361 actcatcagg gcctggttga tgtccaggga tgccccaccg gggtaaccaa aaacatgtgt
      421 gaccccttct ctctcaagtg atttggcgat gatctcgcta cccttttgtg tgatcctcat
      481 gacccaagcc cctacacccc taccccccca aggtcaactc cacttctctg aggtgcttga
      541 tctgatcgcg gagttgcgcg gcctcttcat agtccatttt ttgagcacag atctgcatct
      601 gtttttttag tttgaggatc agaccgggaa tatcacgaac attcatgacc tgatcgcctt
      661 gcaaggcctt cgggatatcg atgtaatcgg ccttgatctc atgcggcaac ccgtctcgta
      721 tggccttgct cacagtcgtg ggagtgatgc tgttctcacg attataggcc aactgttttt
      781 cacgccttct ctgcgtctca tccaaggctc tcttgagaga tccagtcacc gtatcggcgt
      841 aaacaatgac tttcccgttg atgtttcggg acgccctgcc aaaggtttgg atcagggaac
      901 gctcactgcg caaaaatccc tctttgtcac catccagaat cgccaccaag gcgacctctg
      961 gcagatccag cccttcgcga agcaggttga tgccaatgag cacatcgaaa tcacccaaac
     1021 gcagatcacg caaaatctga aaacgttcga tcgtgtcgat atcggaatgt aggtaccgac
     1081 accgaatacc cacctcactg aagtatttgg acagattttc tgcctgacgt ttggtcagcg
     1141 tggtcaccaa aacccggttt cccgtgttca ctgtttcttt gatagcggcc atcaggtcat
     1201 ccacctgatt gttgactggc ttgacctgca cttggggatc caccaatcct gtgggtcgaa
     1261 tcacttgctg aatcacttgg cctggcgttt gctgcagttc atagctccct ggcgtggctg
     1321 atacgaacac cgcttggtcc aagcacgctt caaactcctc aaaggtgagt ggccggttgt
     1381 cgagagcgct cgggagacgg aaaccatatt cgatgagcgt ctgtttacgc gccctgtctc
     1441 cgtgatacat gcccccgatc tgggggacag agatgtgcga ctcatccaca aacagcaaaa
     1501 aatctctggg aaaatactgc aacagagtcg gcggtgggct tcccggcagc ctttggtcga
     1561 aatggcgcga ataattttcg atccccttgc aaaagccaac ctcctcgatc atctccaagt
     1621 cgtactgggt gcgctgttgc aggcgttgtt tttcgaccag cttcccctgt tggtcgaaag
     1681 actcaagtct ctgctcaagt tccaaacgaa tcgacgcgat ggcctgcatt tttttttctt
     1741 cgggcaagac gtaatgtgat cccgggtaaa tcgcgatgcg atcgattttg tcgatcacct
     1801 ctcctctgag cgaatcaatg atcttgaggg actcgatctc gtcatcaaaa aactccacac
     1861 gaatggcccg gtcctcctca taggcaggaa agatgtcgag gacatcacca cgcgaccgaa
     1921 atacacctcg atgaaaatcg atatcgttac gttcgtatcg caaggtcacc aatttattga
     1981 tgacctcaga ccgcgagatg ttcatgccct tccgcacgtc gatgaggagg ccttgataag
     2041 cctccggtga tccgagtccg tagatacagg aaacactggc cacaatgatc acatcggaac
     2101 gcgtgagcag ggagtgtgtg gcggcatgcc tgagtttatc gatgtcgtcg ttgatggatg
     2161 aatctttttc gatgtacaga tcatttcgtg ggatgtaagc ctcgggttga aaataatcat
     2221 aataactcac gaaatattcc accgcatttt cgggaaaaaa agatttgaac tctgaataga
     2281 gttgcgccgc cagggtttt
//
LOCUS       DATDUN010000705         1387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_494431, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000705 DATDUN010000000
VERSION     DATDUN010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494431"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VJC18_09785"
     CDS             <1..797
                     /locus_tag="VJC18_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RRM3/PIF1 helicase-like protein"
                     /protein_id="HLD45715.1"
                     /translation="PISANSRTKDWAFLSHAWQQTEFENILLKTIVRTREKEFIDVLN
                     EIRIARPTSLARDFLNSKTEPFVEDYDGTCLFPRRQQTDGFNIKKLAQIDSAPRIFPT
                     KYEGRKEAIESLKKQAPIPPVLELKIGALVMLRQNDPAYRFINGTTGHVIDIETDRIS
                     IELKNKRVIEVEPSEFSLLDADGEVAASAFNFPLCLAYALTIHKAQGTTLDEALIDLR
                     NLWEPGQAYVALSRLSSSDGLKLIGWDESSILADEQVTRFYENIER"
     gene            complement(948..>1387)
                     /locus_tag="VJC18_09790"
     CDS             complement(948..>1387)
                     /locus_tag="VJC18_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HLD45716.1"
                     /translation="RLTIADAFVYANKKKPDYLVDIATLTGACLVALGARYTGIMGND
                     KDLVSALIKAGDAVGEKMWELPLIEEYREEMKSPIADYKNSGTGYAGAITAGLFLEGF
                     VGKTKWAHLDIAGPSWTDKPLAYTPRGGTGVMVRAMAKFLQDL"
BASE COUNT          370 a          375 c          308 g          334 t
ORIGIN      
        1 cccccatcag tgccaattct cgcaccaagg attgggcctt tctaagtcac gcctggcaac
       61 aaaccgagtt tgaaaatatt ttactcaaga cgattgtgcg cacacgtgag aaagaattca
      121 tcgatgtctt aaatgaaatc aggattgcca ggccaacaag tcttgcccgc gattttttaa
      181 acagcaagac cgaacccttt gtggaagact acgacggcac ttgtctcttc ccgcgtcgac
      241 aacaaaccga tggcttcaat ataaaaaaat tggcacaaat cgatagcgca ccccgtatct
      301 tccccaccaa gtatgagggc agaaaagagg ctattgaatc actgaaaaag caggccccca
      361 tcccgccagt cctcgaactc aaaatcggtg ctctggtgat gttacgccag aacgaccccg
      421 cctaccggtt catcaatggc accacggggc acgtgatcga catcgaaacc gatcgcatca
      481 gcatcgaact caaaaacaag cgtgtgattg aggttgaacc ttcggaattc tccttgctcg
      541 atgccgatgg cgaagtggcc gccagcgcgt ttaacttccc attgtgtctg gcctatgcat
      601 tgaccataca taaagcccaa ggcaccacgc tcgacgaggc attgattgat ttaagaaatt
      661 tgtgggagcc cggccaagcc tatgtggcct tgagtcggct gtcgtcgagc gacgggttaa
      721 aattgattgg ttgggatgaa tcatcgatcc tggccgatga acaggtgaca aggttttatg
      781 aaaatattga acgataagtt tggcattgct tcacgctgat gactgaggtg tgacatagag
      841 tatgtcattc ctgcgaaagc aggaatccat attttcaaca attctggatc cctgcgttcg
      901 cagggatgac aaaattcgac attccttgtt cgatattcaa atgggtgtta taaatcctgc
      961 aaaaatttgg ccatcgccct caccatcaca cccgtcccac ctcggggcgt gtacgccaaa
     1021 ggtttgtctg tccaggaagg acccgcgatg tccaaatggg cccacttggt cttgccaaca
     1081 aacccttcta aaaaaagacc cgcggtaatg gccccggcat aacccgtacc tgaattttta
     1141 taatcagcaa tgggagactt catctcttca cgatattcct caatcagggg caactcccac
     1201 attttttctc ccacagcgtc ccctgcctta atgagcgcgc ttacaagatc tttatcgtta
     1261 cccataatcc ctgtgtacct cgcccctaac gccaccagac aagcccccgt cagggtggca
     1321 atgtccacca gataatctgg cttttttttg ttggcgtaca caaaggcatc ggctatcgta
     1381 agacgtc
//
LOCUS       DATDUN010000706         1523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_614641, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000706 DATDUN010000000
VERSION     DATDUN010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614641"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VJC18_09795"
     CDS             <1..613
                     /locus_tag="VJC18_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HLD45717.1"
                     /translation="AVENARLAVGDNILLAGNLAGMNPSPTTGAKPEDYAQHYFDQAS
                     LMAGEGVDLVVVETITNFIDLRAALVGCQAALGNKTPWVVSVVPRQNGALLDNTAVTE
                     WVPEIKAGGASGAGLNCFCEMRAVCSVVSALDALAPFPLFVKPNAGIPHKTDNRWVYP
                     VTEKEFCNEIQKLLGLPITVVGGCCGTDPHYIKELAALLTSGS"
     gene            659..>1523
                     /locus_tag="VJC18_09800"
     CDS             659..>1523
                     /locus_tag="VJC18_09800"
                     /inference="COORDINATES: protein motif:HMM:NF020928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HLD45718.1"
                     /translation="MELIETISHETHVKPDFIKAILQLHAEGCTIPFMTRYRKEKIGL
                     SNEFVVKQIISRHEELKMLSKKQKEVLQKLTDAGELSEGLKKYIQTATNEYELEELEQ
                     SFQPRAKGPQITLTRTALKPLIEIILKQENITVPLEALASNYVNEPSGLVSPDKVIEG
                     LRKILAEELLRTPSLRTDIKNIYWSEALFQCSKTTEKDISSKFSEFDHFAKPAKDITP
                     HQFLTIQTGVRQKQLKFTVTADDQTVLERLFARRISTQEPTIRFLLENVYSELYHEPF
                     KNNLDAYVVKKL"
BASE COUNT          421 a          430 c          355 g          317 t
ORIGIN      
        1 cgctgttgaa aatgcccgtt tagccgttgg tgacaacatt ttgctggcag gcaatctggc
       61 gggcatgaac ccctctccca cgacgggagc taaaccggag gactacgccc agcactattt
      121 tgaccaggcc tccctgatgg caggcgaagg tgtagacctc gtggtcgtgg aaaccatcac
      181 gaattttatt gacctgcggg ccgctctggt cgggtgtcag gccgctttgg gcaacaaaac
      241 accctgggtt gtttcggtcg tgccacggca aaatggggcc ctgctggaca acacggcagt
      301 gactgaatgg gttcctgaga tcaaggctgg gggtgcctct ggggctgggt tgaactgttt
      361 ctgcgaaatg agagctgttt gttctgtggt ttcggcactg gatgcgcttg cccccttccc
      421 cctttttgta aagcccaatg cggggatccc ccataaaacc gacaaccggt gggtttaccc
      481 tgtcacagag aaagagtttt gcaatgaaat acaaaaactg ctagggcttc ccatcacagt
      541 ggttgggggg tgttgtggca cagaccccca ctacattaag gaactcgcag ccctattaac
      601 cagcggttcg taagtcccgt atgtccctac gcgacacgcg attcacgaat acagaatcat
      661 ggaactcatc gaaaccatca gtcacgagac tcacgtcaaa ccagacttca tcaaggccat
      721 cctgcaactc cacgcggaag gttgcaccat ccccttcatg accagatacc gcaaggaaaa
      781 gatcggcctc tccaacgagt tcgtagtcaa acagatcatc tcgcggcacg aagaactcaa
      841 gatgctctca aaaaaacaaa aagaggtcct gcaaaaattg acagacgcgg gagaactcag
      901 tgagggactc aaaaaataca tccagacagc aacaaacgaa tacgagctgg aagaactcga
      961 acagtccttc cagccacgcg ccaaaggccc ccagatcacg ctcacccgaa cagccctcaa
     1021 gcccctgatc gaaataatcc tcaaacagga aaacatcacg gtgccgctgg aagccctggc
     1081 cagcaattat gtgaacgaac cctctggtct cgtcagtccc gacaaggtca ttgaaggact
     1141 acgcaagatt ttggccgaag aactcctgcg cacaccctct ttgcgaaccg acatcaagaa
     1201 catctactgg tccgaggccc tttttcaatg ttccaaaaca accgaaaaag acatttcctc
     1261 aaaattctct gagttcgatc atttcgcaaa acccgccaaa gacatcaccc ctcaccagtt
     1321 tttgaccatt cagacggggg taagacaaaa acaactcaag ttcactgtga cagcagacga
     1381 ccaaacagtt ctggaacggc ttttcgctcg ccggatttcg acgcaggaac cgaccattcg
     1441 ttttctgctc gaaaacgtgt attccgaact ttaccacgaa ccgttcaaga ataacctcga
     1501 tgcctatgtg gtaaaaaaac ttt
//
LOCUS       DATDUN010000707         3368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_404274, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000707 DATDUN010000000
VERSION     DATDUN010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404274"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VJC18_09805"
     CDS             complement(<1..1134)
                     /locus_tag="VJC18_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45719.1"
                     /translation="MVGKVSTQQAGVQTDAQKAEIPVSLQVPVVPITSSRVALPDREV
                     PIATDTFSLQTGAAPVQPGVEVTRGFKRFAGSASEVWSGVTKDPAVQALLSEVNGRDF
                     TTAWQGVPSDFRRVLDAHPKGAIGFGIVLSDRVKVRLYEAWQQLPDGERLPPKRIHKD
                     MERLRQIMAFVGQNYPDEALDLPYTEITLKALTELMRGDRESVTEICNWLDTFGFEIQ
                     IRELEQQNRQPLEQIAIRRQELVDHQAALQTLQGEMDRLRGDQGEWWNPWDADHEQEI
                     AELQSRILAVQDEIASYQDSIPEWQAEIDAVQSQINETLWKLLVGRGGTQETLDQFLY
                     AISYNPSQVPEALKGKLPAAKARLAKTAADGSVEKLLLTGQYED"
     gene            1794..2906
                     /locus_tag="VJC18_09810"
     CDS             1794..2906
                     /locus_tag="VJC18_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45720.1"
                     /translation="MNPFRYGRVVNEEYFCPRPELIKTLHQNILSAQNVVVQGERRTG
                     KTSLIWQACTSKKKSLILYADFLEVKTTEDVVRRLLNALATYEQQAGLFQKLFKALSH
                     LKPQISIDPYSGEPSFSFDVSSKATPDSVEHILDLYATLSHRKPLVVVFDEFQDILNL
                     VDHASLLACMRGKVQFHTKIPYIFSGSVRNRMEEIFTHQDSPFFKSALPLWVGAIDDD
                     VFAHFLKNKFALEKRKIDSNLMTKIFQLSEHNPGDTQQLCSALWEITESGGVVSEDLL
                     PEAMQVIFSREAKTYESLLVSITGIQLKCLVGLARFETLSPQSSEFVRNTGLSGPASA
                     YKALLRLEQIKIVYRTPQGYKFSNPYFKQWLLFKKY"
     gene            complement(2937..3263)
                     /locus_tag="VJC18_09815"
     CDS             complement(2937..3263)
                     /locus_tag="VJC18_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45721.1"
                     /translation="MKNIFMTKRTMLLIFVLMLFLAGGLCFFLNRDHCASIIDSSERV
                     QCTIELAEKEHNLHRCHLNYYTLACIQKADPQHTAKKEDVGKLCAAITDSSTRADCEQ
                     YVANHY"
BASE COUNT          853 a          836 c          758 g          921 t
ORIGIN      
        1 gtcttcgtat tgacctgtga gcagtaattt ttcgacagag ccatcggcgg ctgtctttgc
       61 caaacgtgcc ttggccgcgg gaagcttgcc tttgagggct tcgggaactt gagaggggtt
      121 gtagctgatc gcatacagaa actggtccag ggtctcctgt gtgccgcctc ttcccacgag
      181 cagtttccac aacgtctcgt tgatttggct ttgcacagcg tcaatttctg cttgccactc
      241 agggattgaa tcctgataag aggcgatctc gtcctgaacg gccaagatcc ggctttgaag
      301 ttccgcgatt tcctgttcat gatcggcgtc ccagggattc caccactcac cttggtcacc
      361 acgaagacga tccatttctc cttgcagggt ttggagcgcg gcttgatgat ccacaagctc
      421 ctggcgacga attgctatct gttccagcgg ttgtcgattt tgttgttcga gttcccgaat
      481 ctggatttca aaaccaaagg tgtccaacca attgcaaatt tctgtgaccg actcgcgatc
      541 gccgcgcatg agctccgtca aggccttgag ggtgatttcg gtgtacggaa gatccaaagc
      601 ctcgtcggga taattttgcc ccacgaaagc catgatttgc ctcaggcgtt ccatgtcttt
      661 gtggatccgt ttcggaggta atctctctcc atcgggaagc tgttgccagg cctcatagag
      721 acggactttc acgcggtcgg acaacacaat tccaaacccg atggcccctt tgggatgcgc
      781 atccagaacc ctgcgaaaat cggacgggac gccctgccag gctgtcgtga aatccctgcc
      841 attgacctcg gagagaagcg cctgaacagc ggggtccttg gtcacccccg accacacttc
      901 actagcggaa ccggcgaaac gcttaaaacc tcgtgtgact tctaccccgg gctgaacggg
      961 agcggcccct gtctgcaggc tgaaggtatc ggtggcgatt ggaacttctc tgtccggaag
     1021 ggcaacccgt gaactagtga taggaacaac agggacctgc aaagagacgg gaatttccgc
     1081 tttctgagca tcagtttgca ccccagcttg ctgtgttgaa accttgccaa ccatttctgt
     1141 gtctcctcat cgatcgaagg ccacttaccc atcttgcaac ttccaagcca acgagctcga
     1201 cggcactcag tttttttgcc caatgatttc aacagattgc ccccttggcg ttcctccgtc
     1261 atacgttttc caagtcaaat aagtagcttt gttatcgttt tgataagctt ttgatctgat
     1321 gtgaactgac gtgccccccg gccatcgctt gtctacacat gcaaataaga gaccaaatta
     1381 cgacacaatt tgtcgcataa atatttgtgt gggtttttcg agggttaaag aaatggagag
     1441 attgcggttt gagtttgctt atgaaacaaa tgacaataac tggtgattgc tgtctgctgt
     1501 taagggctgt tttgggggct ggggtaatcg acatcgacta aaacacggat catttgtgtt
     1561 ttttctcgat tacgtattaa gcgcaccatc atcgagtata atgtcaatat accctttcaa
     1621 aaatgtgctc accagagctc tgcaaggcag acatagaatg cgtgggcagt gactgtacct
     1681 tttaagggta aaagatgact gacactatta aatcgcttgc cgttaatatt accattgtgt
     1741 atgatactat ttagtatgat acacaatggt aatatttcaa gaagggagag atcatgaatc
     1801 cttttcgtta cggtcgcgtt gtgaacgaag aatatttttg tccgcgccca gaactcatca
     1861 agactttaca tcaaaacatc ctttcagctc aaaacgtcgt ggtccaaggc gaacgccgca
     1921 cgggcaaaac atcgctgatt tggcaagcct gcacctccaa aaaaaaatct ctcattcttt
     1981 atgctgattt tcttgaagtc aaaacaaccg aggacgttgt tcgtcgtctg ctgaacgcgt
     2041 tagccacata tgaacaacag gccggattgt ttcaaaaatt attcaaggcc ttgtctcatc
     2101 tcaaacctca aatttcaatc gatccctatt ccggagagcc atcattcagt ttcgatgttt
     2161 cttccaaggc cacaccggat tccgtagaac acatcctgga cctttacgcc accctctcgc
     2221 accgcaagcc tctggttgtg gtctttgacg agtttcagga tattctcaat cttgtcgatc
     2281 atgcaagcct gctggcctgt atgcggggaa aagtccagtt tcacacaaaa atcccttaca
     2341 ttttttctgg aagtgttcgc aatcggatgg aagaaatctt cacacatcag gacagcccct
     2401 ttttcaaatc tgccctgccc ctctgggtgg gagccataga cgatgatgtt tttgcccatt
     2461 ttttgaaaaa taaattcgcc ctggaaaaac gtaaaatcga ctcaaatctc atgacaaaaa
     2521 tttttcaatt gagtgagcac aaccctggtg acacacaaca gttgtgttct gcgttgtggg
     2581 aaattacaga atcggggggt gttgtttcgg aagatttgct tcccgaggcc atgcaagtca
     2641 tcttttctcg cgaagccaaa acctacgaat ccctgctcgt gtccatcaca ggaatccagc
     2701 tcaaatgttt ggtaggtctg gctcgcttcg aaacactttc accgcaatca tcggaattcg
     2761 tccgcaacac tggtttgtcc ggacccgctt cggcttacaa ggccctgttg cggttggaac
     2821 aaatcaagat cgtctatcgc acaccacagg gttacaaatt ctcaaacccg tatttcaagc
     2881 aatggttgtt gttcaaaaaa tattaaaaac cttcaaaaca aatttaaggc taacggctaa
     2941 taatgattcg ccacgtattg ctcacaatca gctctggtag aagaatcggt gatggccgca
     3001 cacaatttac caacatcttc tttcttggct gtgtgttgcg gatcggcttt ttgaatgcaa
     3061 gccagcgtat aatagttgag gtgacagcga tgaaggttgt gttctttttc cgccaattca
     3121 atggtacatt gcacgcgctc tgacgaatcg atgattgagg cacaatgatc gcggttgagg
     3181 aaaaagcata acccccctgc cagaaaaagc atcaacacaa aaattaacag catcgttctt
     3241 ttcgtcatga aaatattttt catatcggtc ccgccttatc tacaaatcca gcatgcgttt
     3301 tgtagagtca ctcgtaaaat cattcagata tttattattc agatcctctc ttgataatga
     3361 atcaagag
//
LOCUS       DATDUN010000708         1258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_77874, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000708 DATDUN010000000
VERSION     DATDUN010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77874"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VJC18_09820"
     CDS             complement(<1..284)
                     /locus_tag="VJC18_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HLD45722.1"
                     /translation="MDTLITTILKRPYVVLFLLSYIIIATRVVGWRWMISFLIAGYTI
                     AFTSEFLSINTGFPYGWYFYKYENLQGEWLNNGVPVWDSGSYVFMCFAGM"
     gene            393..836
                     /locus_tag="VJC18_09825"
     CDS             393..836
                     /locus_tag="VJC18_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45723.1"
                     /translation="MSRFYFALCFCFFCLFTTNIQANEETHALGNTGYMIREAMHEQA
                     NRKDSTDYFGAIKMQNEAKAYMHGTHKLGRHKQKYRELAQSAYELAKQARDSSIAEKA
                     NCTVQNARCKMKESAVVSKGSLKYRRDVNQFSTPFGKKNSSASGR"
     gene            910..>1258
                     /locus_tag="VJC18_09830"
     CDS             910..>1258
                     /locus_tag="VJC18_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HLD45724.1"
                     /translation="MFQSNLPQFKLLFRGKVRDVYDLGDELLIVATDRISAFDVVLPK
                     PILGKGIVLTRLTEFWMDKFKDLIVNHMSLKKLRDVLTDDSLYQEYKDRTVVVKKAKS
                     LPIECVVRGYLLGT"
BASE COUNT          361 a          278 c          296 g          323 t
ORIGIN      
        1 tacatccccg caaagcacat gaacacatat gaacccgaat cccacacggg cacgccgttg
       61 ttgagccact ctccttgcag gttttcgtat ttgtaaaaat accagccgta aggaaacccc
      121 gtattgatcg agagaaactc actggtaaag gcgattgtat aacctgcgat taaaaaagaa
      181 atcatccaac gccagccgac aactctggtg gcgatgatga tgtacgataa caaaaacaaa
      241 acaacgtagg gacgtttgag gatagtggtg atgagggtgt ccatgacaat gcgcaattat
      301 agcaattgtt gcaatttggc aattctgcaa tggcgaatcc cagtgaacat gattgactgc
      361 cactcattat tcgcttagtc gtcatcaata tgatgagtcg tttttatttc gcattgtgtt
      421 tttgcttttt ctgtctgttc accacgaata tccaggccaa tgaagaaacg cacgcactgg
      481 gcaatacggg ctacatgatc cgagaggcca tgcatgagca ggccaacagg aaggattcga
      541 cagattattt tggggccatc aaaatgcaga atgaggccaa agcttacatg catgggactc
      601 acaagttggg cagacacaaa caaaaatatc gagaattggc acagtctgcc tatgagctgg
      661 ccaaacaggc gagggactct tcaattgcag aaaaggcaaa ttgcacagtg caaaatgcaa
      721 gatgcaaaat gaaagagagc gcagttgtgt caaagggttc cttaaaatac agaagagatg
      781 tgaatcagtt ttcgacaccc tttgggaaga agaattcgtc tgcctctggc cgttagctgt
      841 tagccgttgg ccgttggcct tacgccttaa gcctttggtc ttgcgcctta cgccttacgc
      901 cttttatata tgttccaatc aaacctacca caattcaaat tattattcag aggcaaagtt
      961 cgagacgtat acgatctggg tgatgaactc ctgatcgtgg ctaccgatcg catttccgcg
     1021 ttcgatgtcg ttttgccaaa acccattttg ggcaagggca ttgtgctcac caggcttact
     1081 gagttttgga tggacaagtt caaggatctt attgtcaatc acatgtctct caaaaaactg
     1141 cgcgatgtgc tcaccgacga ctctctgtat caagaatata aggatcgcac ggtcgtggtc
     1201 aaaaaggcca agtctttacc cattgaatgt gtggtgcgtg ggtatctttt ggggactg
//
LOCUS       DATDUN010000709         5229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_200568, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000709 DATDUN010000000
VERSION     DATDUN010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200568"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VJC18_09835"
     CDS             complement(<1..885)
                     /locus_tag="VJC18_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLD45725.1"
                     /translation="MFEPKYHISDKLLNNIKQISVVMADVNNRSFSDVVLADFEQRAN
                     SLSAHASTSIEGNPLPLTDVKRLIKLRPENIRDTEREVLNYNEALESINEKTLSGRVI
                     MTIKFICETQKLVTKGLIEKFRCGKLRNEPVFVNDPRVGKPVYLPPDHQDVPQLMNEL
                     LDFVKTKRGLLDPLILAGLFHKQFVIIHPFIDGNGRTTRLVTKALLADLGLNTFRLFS
                     FENFYHRNVSRYFKTVGVLGNYYKIINQIDFTEWLEYFTDGILDELLRVKKELGESGA
                     TPDTVLQSHHKKILSVIEK"
     gene            1130..2026
                     /locus_tag="VJC18_09840"
     CDS             1130..2026
                     /locus_tag="VJC18_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45726.1"
                     /translation="MATTIPQNPKSYDAEYDLPIALGITQTEQEDMISRLSDEVISYS
                     QATGKSFNTGGDSFINQPPDPRIRLGGYGTDGKKFYDGVGATEHAAIIQPTIKFMEGM
                     GYQVKAVTVGSHGYTFDLSFNGKKVEAELYSEKYSGVSSDFLTRLEHTADVLKNSSAG
                     SACKWVRVNSNSDSVDFVLPEHREIMEEHKIGDTPDSSAGSIWVNNKHLEPAAFTSAY
                     KVYTALHDRVPHFAHQTEYTRDGTVAARVAPNISQIWRTPIGTVVTISEPKDSTAAQI
                     RDEFTTGASQFYPQSQPLVAEE"
     gene            complement(2037..3200)
                     /locus_tag="VJC18_09845"
     CDS             complement(2037..3200)
                     /locus_tag="VJC18_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HLD45727.1"
                     /translation="MSSVASDSTEIPLALLWDWQRLDLVDESLTDIGEMQCYPRYDDS
                     AVSSLNPDVQEYAQYLKALFSQTDESTSRPQLLTDYIDGVQCVRHNSPDQTRQLWTAD
                     LSLANSPEAQTFVLENLCWLDLFTGAWDMLATDTKFLLNAAPWSSVGLYASASLAFRE
                     HQFDVALTELNKIDEAFASPVLRTTARTLRTKITVRQETREQVLRRQSTLQNFTRDEQ
                     EIYLSQASYHVQSNADLYLDVFRSQISNCNDLISCLRSWLKQQPQQEAYALLPQLLHI
                     FFRSRWAYHNQYTSPDEDNSDRCFESRQYNCADYAMIMAKVLKPFGIESRLVSYYSSP
                     ENLGHMFVAFKVDNSWGFGNPSSFHPARFKTLDDVLNQWGWANDLGEFVVRET"
     gene            3501..4157
                     /locus_tag="VJC18_09850"
     CDS             3501..4157
                     /locus_tag="VJC18_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HLD45728.1"
                     /translation="MKKLTPVAKRRLIQWILAPITIITIGLGWKYPILGFFVPLTMLA
                     GIIGSLFNGRWVCGHMCPRGSFFDRILSKISPVRTVPAFVRTMKFRWPIFGLLMGFMS
                     FRIMQNPGSWQHWGYVFWVMCVVTTAIAIVLGVIVHPRIWCSFCPMGTMQNAIGGGKN
                     LLRINGDICKMCKTCEKACPIGIPIVSYKNDGVVRDRDCLKCPECLAVCPKKALSWNQ
                     "
     gene            complement(4168..>5229)
                     /locus_tag="VJC18_09855"
     CDS             complement(4168..>5229)
                     /locus_tag="VJC18_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLD45729.1"
                     /translation="PTHGIQVQSWTDHVWLYNNTIKNTDSSGIISMYNKNVLVEANSV
                     TEACASGIHENVSMGYTEGFAVIDNTIFDGWHDEKFCTALSEDGYIGHNEIYGRGLTG
                     VYVASGSEKLRVDGNRIHDLTQNTCLLIESEDAGPIRDIIFENNLCYNNPAGFGIAVT
                     QYNCAKNQPGDGGVALFACEYDENMDYENLIITNNTIYQTGMGTTMGYGLKLDAGSAQ
                     NLVVANNIFSNNVTAQVAPHNHMLDSEGKLKSNVIMDNNLIFCEGNEDANQDGYQDCL
                     NVSDRWYTGALEGYSAYGPGLFADPYFVNPIAFDFSLQAASPALNTAVEEHYVPAYDQ
                     NCHVRQAGQVDLGAFERIP"
BASE COUNT         1419 a         1278 c         1165 g         1367 t
ORIGIN      
        1 tttttcaatc actgacaata tttttttgtg atgcgattgc aaaacagtgt ctggagtcgc
       61 tcccgattcc cccaactctt ttttgacccg caacaattcg tccaaaatac cgtcggtaaa
      121 atattccagc cattctgtga agtcgatttg gtttatgatt ttgtaataat tgccaagcac
      181 ccccacggtc ttgaaatacc tgctgacatt cctgtgatag aaattttcaa agctgaaaag
      241 cctgaacgtg ttcaaaccca aatcagccag caaggccttg gtaaccaaac gagtcgtcct
      301 cccgttgcca tcgatgaacg gatgaatgat cacaaactgt ttgtgaaaga gtcccgccaa
      361 aatgagagga tccaaaaggc ctcttttggt cttcacaaaa tccaacaatt cattcatgag
      421 ctggggaacg tcttgatgat ctggaggcaa ataaactggt ttgcccacac gcgggtcgtt
      481 gacaaaaacg ggttcgtttc tcaattttcc acaccggaac ttttcgatga gtcccttggt
      541 caccaatttt tgtgtctcac aaataaattt gatggtcatg atgacacgac ccgagagggt
      601 tttttcattt attgattcga gcgcttcatt gtaattgagc acctcgcgtt cggtgtcgcg
      661 aatgttttcc ggcctcaatt tgatcagcct cttcacatcc gtgagtggca agggattgcc
      721 ctcaatgctc gttgaggcat gggccgataa tgaattggcc ctctgctcaa aatcagccag
      781 aacaacatca gaaaatgatc ggttgttcac gtccgccatc acaaccgaaa tttgtttgat
      841 attgttcagc aatttatctg aaatgtggta tttgggttca aacatatgcg tctaattata
      901 gacgataaat agacgattgt aaaggcctcc atgccaagac caaaacaaac acatgtactt
      961 ttcagccatc gactaagtga tttctctctc aaaatgagag aggcgtcaca ctctagtcat
     1021 ccattcacac cattcaaaaa ataactcctg ataattattg cctatttcta caatgaaact
     1081 ggcataaaaa ttgaaacata agccatgttt gaccaaaaaa ggagtcacta tggcaactac
     1141 cataccccaa aaccccaagt catatgatgc tgaatatgat ttgccaatag cgttgggtat
     1201 cactcaaaca gaacaggagg acatgatcag tcgattatct gatgaagtga tctcctatag
     1261 tcaagccact ggtaagtctt ttaatacagg aggggatagt tttataaatc aacctcccga
     1321 cccaaggatc cgtttgggag gttatgggac cgatgggaaa aaattttatg atggtgttgg
     1381 tgcaaccgaa catgccgcta tcattcaacc cactatcaaa tttatggaag ggatggggta
     1441 ccaggtaaag gctgtgacag ttggctctca tgggtacacg tttgatttga gcttcaatgg
     1501 taaaaaagtg gaagcagaat tgtactctga aaaatacagt ggagtctctt cggattttct
     1561 gaccagactg gagcacacgg cagatgtatt aaaaaactcc agcgcaggct ccgcgtgcaa
     1621 gtgggtgaga gtcaacagca acagtgattc tgtggatttc gtgttgcctg agcacaggga
     1681 aatcatggaa gaacacaaga taggcgacac accggactcc tctgcgggaa gcatatgggt
     1741 caataacaaa catcttgagc ccgctgcatt taccagtgcc tacaaggtat acacagcact
     1801 gcacgacagg gtgcctcatt ttgctcacca gactgaatat accagggacg ggactgttgc
     1861 cgctagggtg gctcccaata tttcgcaaat ctggagaaca cccattggta ctgtggtgac
     1921 aatttccgaa ccaaaggata gcacggcagc acagattcga gatgaattca caacgggggc
     1981 atctcaattc tacccgcaat cacaaccatt ggttgcagaa gaatgaggca aacaattcac
     2041 gtctccctca ccacaaactc cccaagatcg ttggcccaac cccattgatt caaaacatca
     2101 tccagcgttt tgaaccgagc agggtggaac gaggagggat tgccaaaccc ccatgagttg
     2161 tccactttga aggcaacaaa catgtgtccc aaattttcag gtgaagaata ataagaaacc
     2221 aacctcgatt ctatgccaaa cggttttaat accttggcca tgatcatggc gtaatcagcg
     2281 cagttgtact gacggctctc aaaacaccga tcggaattgt cttcatcggg cgatgtgtac
     2341 tggttatgat aggcccaccg tgatctgaaa aaaatgtgca acagctgggg caagagtgcg
     2401 taagcttcct gctgtggttg ttgtttcaac cacgaccgca aacatgagat gagatcatta
     2461 cagttggaga tctgggaacg aaagacgtca agatacaggt ctgcattcga ttgcacatgg
     2521 taggatgcct gactgagata gatttcctgt tcatcgcgcg tgaagttttg tagcgtagac
     2581 tgcctccgta aaacctgctc tcgcgtttct tgcctcacag taattttggt tctcagtgtc
     2641 ctggctgttg ttcttaaaac cggtgaggca aaggcctcat cgattttatt caactcagtc
     2701 aacgcaacat caaactgatg ttcgcgaaag gccagagacg cgctggcata caaccccaca
     2761 gaactccagg gggccgcgtt taagagaaat tttgtgtctg tggccagcat gtcccaggca
     2821 cccgtaaaca aatccagcca gcagaggttt tccaacacaa acgtctgtgc ctctggtgaa
     2881 ttggccaatg acaaatccgc cgtccaaagt tgtcgcgtct gatccgggga gttatgacga
     2941 acgcattgaa ctccatcaat gtaatcggtc agcagttgtg ggcgtgacgt ggactcgtct
     3001 gtttgagaaa aaagtgcttt gaggtattgc gcgtactctt gaacatcggg gttcagagaa
     3061 ctcaccgcag aatcgtcata acgcgggtaa cactgcattt cgccaatatc cgttaaggat
     3121 tcatcgacaa ggtctagtct ttgccaatcc caaagaaggg ctaaggggat ctctgtactg
     3181 tctgatgcaa cggatgacat aggaatctca aaaaaaatta ataagccggc tcaacgcccg
     3241 caagtcatac tcggcacaca cgccaaaaag ttgcgtcctg ttcttgcctc attcccctct
     3301 tttttcccat tctcattttg aaaacacctc attttgagat accttcttgg ccagaacaaa
     3361 gcagtaccgc tttgaaataa cttgttattt ctccagcatg ggaatgggca acgccagtgt
     3421 tcagcccaat atcaacctgg atgcccccta gaaattctca acccttgaca tccccccatt
     3481 cgtgcacatg atggcagtct atgaaaaaac tcacacctgt tgccaagcgc cgcctcattc
     3541 aatggattct cgcccccatc accatcatca caatcgggct tggctggaaa tacccgattt
     3601 tggggttttt cgtaccgctc accatgctgg cagggatcat tggcagtcta ttcaatggtc
     3661 gctgggtttg cggccacatg tgtccacgtg gcagtttttt tgaccgcatc ctctcaaaaa
     3721 tttcaccggt cagaaccgtg cctgcgtttg tccgcaccat gaaattccgc tggcccattt
     3781 ttggcctgct catggggttc atgtcttttc gcatcatgca gaatccgggt agctggcaac
     3841 actggggata tgtcttttgg gtgatgtgcg tcgtgaccac agcgattgcc attgttctgg
     3901 gcgtcatcgt ccatccccgc atctggtgct ctttctgccc catgggaacc atgcaaaacg
     3961 ccatcggggg tggtaaaaat ctgcttcgca tcaacggtga tatttgtaaa atgtgcaaga
     4021 cctgcgaaaa ggcctgcccc atcggcatcc ccatcgtctc ctacaaaaat gatggcgtcg
     4081 tgcgggaccg cgattgcctc aaatgccctg agtgtctggc cgtctgtccc aagaaggcac
     4141 tcagttggaa tcagtaagca aataccctta aggaatgcgt tcgaatgccc ccaggtccac
     4201 ttgcccagct tgacgcacat gacaattctg gtcatatgct gggacataat gttcctccac
     4261 tgccgtattg agagcaggag aagccgcctg cagagaaaaa tcaaacgcaa tggggtttac
     4321 aaaatatggg tcggcaaaca ggcccggacc ataagctgaa taaccttcca aggcacctgt
     4381 ataccaacga tcactgacat tcaggcaatc ctgataacca tcttggttgg catcttcgtt
     4441 gccctcacaa aaaatcagat tattatccat gatgacattg cttttgagtt tcccttcgct
     4501 gtccagcata tgattatggg gagcaacttg ggcagtcacg ttgttgctaa aaatattatt
     4561 ggccacaaca agattctggg cgctaccggc atccagtttt aagccataac ccattgtcgt
     4621 gcccattccc gtttgataaa ttgtgttgtt ggtgatgatg agattttcat agtccatatt
     4681 ctcatcatac tcacaggcga acagggcaac accaccatca cccggttggt ttttggcaca
     4741 attgtattgt gtgacagcaa tcccaaaacc tgcagggttg ttgtaacaga ggttattctc
     4801 aaaaataata tctctgatgg ggcccgcgtc ttcagactca atgagcaaac acgtattttg
     4861 tgtgaggtca tgaattctgt tgccatcaac acgcagtttt tctgagccac tggccacgta
     4921 aacacccgtg agccctctcc catagatttc gttgtggcca atatacccat cttccgaaag
     4981 cgcggtgcag aatttttcgt catgccagcc atcaaaaatg gtgttgtcaa taacggcaaa
     5041 cccttctgtg tagcccatgc ttacattttc gtgtatcccg ctggcacacg cctcagtcac
     5101 tgaattggcc tcaacaagga cgttcttgtt atacattgaa atgatgccag acgagtccgt
     5161 attcttgatc gtattattgt agagccaaac atgatcggtc caagattgaa cttgaatacc
     5221 atgggttgg
//
LOCUS       DATDUN010000710         1804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_321767, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000710 DATDUN010000000
VERSION     DATDUN010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321767"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VJC18_09860"
     CDS             complement(<1..355)
                     /locus_tag="VJC18_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45730.1"
                     /translation="MVSAIFSAISCSKQAGETGSPNSASVATNSATFSCFDVAQNPWM
                     SSYDINQDGYFGEDDLAARVYSSFSGVRIPFLTSTASQLNADCLTAKIGMTSDEVAWK
                     LKSLFANQNACPDPKT"
     gene            complement(495..567)
                     /locus_tag="VJC18_09865"
     tRNA            complement(495..567)
                     /locus_tag="VJC18_09865"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Val,seq:tac)
     gene            complement(777..850)
                     /locus_tag="VJC18_09870"
     tRNA            complement(777..850)
                     /locus_tag="VJC18_09870"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(814..816),aa:Asp,seq:gtc)
     gene            1408..>1804
                     /locus_tag="VJC18_09875"
     CDS             1408..>1804
                     /locus_tag="VJC18_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45731.1"
                     /translation="MTQPRFADRSRYRESALRYKHPPAPTLAELADQHVFDVELETFW
                     RNAGLPRKLLRECFNDPVKKAVLTESFRIGKEFIASLGDDFKTVFSSPVNATALTVTV
                     GAVLMATGCAPGEVTDLVTASGDIWSRLGD"
BASE COUNT          488 a          487 c          401 g          428 t
ORIGIN      
        1 gcgttttggg atctggacaa gcattttgat ttgcaaacag acttttcagt ttccacgcaa
       61 cctcatctga cgtcatccca atctttgctg tcagacaatc agcattcaat tgactagctg
      121 tagaagtcag aaacgggatt ctaacccctg aaaaagagct atagacacgt gccgccaaat
      181 catcctcgcc aaaatatcca tcttgattga tgtcgtatga tgacatccaa gggttctggg
      241 cgacatcaaa acaggagaat gtcgcagagt tcgttgcaac tgacgcacta ttgggtgagc
      301 ctgtttcacc agcttgtttt gaacatgata ttgcagaaaa tattgctgaa accataaaca
      361 ctccttgttg cctgaccatt tcccttttct gacttccgcc ggctggcata ggaaattact
      421 gaacgtttgt aaagcaagct gttttgggct gtttgtgaca gagacagtgt cgacctattg
      481 atgggagatg ggagtgggcg gcacagggct cgaacctgtg actttcacct tgtaagggtg
      541 acactctacc agctgagtta gccgcccgtg caccgctttt gaacatgccc aaaagcctgt
      601 tgcacctaaa ttcagctgtt cgccgaattt aggttgaacg ctgtgcgcca aaaaaaaacg
      661 cgagcatgct ggacaagcgc acggaatatg caaatgtgcc ctcaccagac atgctcgcaa
      721 ggttcgacga acaaatcgcc gatagccatg aaccgtaaac agattcatga caaatgcggg
      781 agcgacgaga ctcgaactcg cgaccttctg cgtgacaggc agacgttcta accaactgaa
      841 ctacgccccc gtgcactgcg atgaacatgc ccaaaggcat gtttagcgct gtgcgccaaa
      901 aaaactatgc aattttcaaa tcttcgggac tttctttaca gttcagttgg ttagaacatc
      961 aacgacgttc actgatatgc tttgtcagcc aaaggctgaa caactgaact acgcctccgt
     1021 gcactgcgat gaacatgccc aaaggcatgt ttagcgctgt gccccctcaa tccctcaagg
     1081 gagatcgctt ttatatgcat cctaaaaaaa aagccagaaa aaaatacgtt gcaaaatcaa
     1141 gcctgaattg cctaaagacg caacgcgacc acaacaaccc gacagacaac aggagtctaa
     1201 cgaatgtatc cagcaagttt ggcaaattcc ttcagcggca aggcaacact cctcagcgat
     1261 tttggacttc caccaccaat ccctttatcg agttccgaaa ccccactcga tagtcccttt
     1321 ttgattcgag ttacgacaac tccggcagga acaggctttc acgaaagtat cgatagtcgt
     1381 ccaactataa ctgtaccccg cccccctttg acccagccaa gattcgctga tcgttcccgc
     1441 tatcgtgagt ccgctctacg ctacaaacac ccgcccgccc ccacactggc cgagctggca
     1501 gaccaacatg tttttgatgt cgagctcgag acattctgga gaaacgccgg acttccccgc
     1561 aaacttttgc gtgagtgttt caacgaccca gtcaaaaaag ctgtcctgac agaaagtttc
     1621 cggataggca aagaattcat tgcctctctg ggagatgatt tcaaaactgt tttttccagt
     1681 cctgtaaatg caaccgccct taccgtcact gttggtgccg ttctcatggc aacgggttgc
     1741 gcgccgggag aggtcaccga cctcgtaaca gcctctggag acatctggtc ccgtctgggg
     1801 gaca
//
LOCUS       DATDUN010000711         1592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_614854, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000711 DATDUN010000000
VERSION     DATDUN010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614854"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VJC18_09880"
     CDS             complement(<1..347)
                     /locus_tag="VJC18_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45732.1"
                     /translation="MARKMVTIDGNEAAGYVAHKINEVCAIYPITPSSPMGEWADEFS
                     AKGLKNIWGTVPKVVEMQSEGGAAGAVHGALQTGALTTTFTASQGLLLMIPNMFKIAG
                     ELTSTVFHVTART"
     gene            complement(477..833)
                     /locus_tag="VJC18_09885"
     CDS             complement(477..833)
                     /locus_tag="VJC18_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45733.1"
                     /translation="MAINTETMTDEEILEQIMEAFEEDGRLSLHYIDFEVVDASITVS
                     GRVSSEEELQIIDELLGEILGLDNYKNKVWVDDSLTYEDPEDNTPDLKDLTFDDDEID
                     DEDYSEEEDEEEGEAF"
     gene            complement(887..>1592)
                     /locus_tag="VJC18_09890"
     CDS             complement(887..>1592)
                     /locus_tag="VJC18_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HLD45734.1"
                     /translation="EARLRSVVIDRVMHALATQYFNIVGILIIFTQPISFPLWFHLLL
                     LAVYVVLCSILTTVIVQLVTGQGFGAFEHLFRWFDVARRFPKVHDVLCELKSNLAYYR
                     DRPKTMFFKAFAYHFAGRVLGAVEIMIAFYAFSGRLDFTFGVILAALTSFFAATFGFI
                     PGALGILEGLYASLFSLYGFSPDMGVSVQIVRRLRVLLWVGVGIGLMDYEIVGVQLKK
                     IKHNIGMKSNTNNLTK"
BASE COUNT          441 a          407 c          357 g          387 t
ORIGIN      
        1 aacgtcctgg cagtcacatg aaaaacagtg gatgtgagct cgccagcgat cttgaacata
       61 ttggggatca tcaacagcaa accctgcgac gctgtgaacg tagtggtcaa agcacctgtc
      121 tgcaaggcac catgcaccgc gcccgccgcg ccgccttcgc tctgcatttc gaccaccttg
      181 ggaaccgtgc cccaaatatt tttgagtccc ttggccgaaa actcatcagc ccattcgccc
      241 atgggggatg agggtgtgat ggggtaaatc gcgcacactt cgttgatctt gtgggccaca
      301 taaccagcgg cctcattgcc atcaattgtt accattttac gtgccatagt gttctccaaa
      361 aaatatgatt tgagattaaa gttttcgcgc gtttaatagt gaaaaaaaac gcgttcagca
      421 agagttaact ggggatgaat ttgagatttt tattgatata aatcaggaat taacggtcaa
      481 aaggcctctc cctcttcttc gtcttcttct tcgctgtaat cctcgtcatc gatttcgtcg
      541 tcgtcaaagg tgaggtcttt gagatcgggg gtattgtctt cggggtcttc ataagtcaga
      601 gaatcatcca cccagacctt gtttttgtaa ttgtccaagc ccagaatctc tcccaaaagc
      661 tcatcgataa tctgaagttc ttcctcactc gacaccctgc cactgacagt gatgctggca
      721 tcaacaacct caaaatcaat atagtgaaga ctcagccgtc catcttcttc gaaggcttcc
      781 ataatttgct cgaggatttc ctcgtctgtc atggtctcgg tgttaatggc cataaacgct
      841 cctaaaaatt ctgttggctt ttcggcgggc atatgatgcg aaacagtcac tttgtcaaat
      901 tatttgtgtt agacttcatc cctatgttat gtttaatttt tttcagttgt acgcccacaa
      961 tttcgtaatc catgagtcca atgccaaccc caacccataa caagaccctg agtctcctga
     1021 cgatctgaac gctgacaccc atatcgggag aaaaaccgta gaggctaaac aggctcgcgt
     1081 acaaaccttc caaaatgccc aaagcgccgg ggatgaaccc gaacgtcgcg gcgaaaaatg
     1141 aagtgagggc cgccaggatc acgccaaaag tgaaatccag tcttcccgaa aaggcataaa
     1201 aggcgatcat gatttcgaca gcgcctaaaa cacggcccgc aaagtggtag gcaaaggcct
     1261 tgaagaacat tgtcttcggt cgatcacggt aataagccaa attgcttttc agctcacaca
     1321 gaacatcatg cactttggga aaacgtcttg cgacatcaaa ccagcgaaac aggtgttcaa
     1381 aggcaccaaa gccttgaccc gttaccaact gcacgatcac cgtggtgagg atcgaacaaa
     1441 gtaccacgta cacagcgagc aacagcaaat ggaaccacag gggaaaggag atgggctgtg
     1501 tgaaaatgat caaaatgcca acgatgttga aatactgtgt ggccaacgcg tgcatgaccc
     1561 gatcgatgac caccgacctg agtctggcct cg
//
LOCUS       DATDUN010000712         4614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_156885, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000712 DATDUN010000000
VERSION     DATDUN010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156885"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VJC18_09895"
     CDS             <1..385
                     /locus_tag="VJC18_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HLD45735.1"
                     /translation="LMFQKEVAERCVASPGNKDYGILSVWSRLLSQPKILFHLPPTVF
                     NPKPKVQSAFVRFDLLGVAYEQEKDFISFVKRVFGQRRKKISTILKKELRHLGTLTNE
                     VATLLDLRAEVLGIDSLKLVFDAIR"
     gene            complement(448..990)
                     /locus_tag="VJC18_09900"
     CDS             complement(448..990)
                     /locus_tag="VJC18_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45736.1"
                     /translation="MSPEPVFPSKKADNKITKELAMFLVPLAAAYLFITFYEAGYSAF
                     FGIPLDLISVNITQVLLTNRLTLMVAVIAFLWIGLYYNILPSTSSPFFKGMISVILIL
                     SFALGIAFGRSDAASQKNFLVSNTEPEQAVLRIYNNTIISASFDRNNKTVFKTFSIRP
                     IDQSNNILFHNETIGPLTPQ"
     gene            1112..2116
                     /locus_tag="VJC18_09905"
     CDS             1112..2116
                     /locus_tag="VJC18_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HLD45737.1"
                     /translation="MSGADSIIIYLYSYFPAITLGLFFWWLDRFERESLGVVVFAFLW
                     GAIGAGLLSFFWNTFFHVLLEVYQKNSFAANEVIIGVVVAPFVEELTKGFVILFLLRF
                     KVVDNVTDGILMGILIGLGFAAAENVFYCQQVVYPNDGELAMWSNLWFRELHTTLLHA
                     SATAVWGAVIGYSRQFRRSQKWLVVLVGFILAVVTHGCWNFFAVYVNQIQSHFNLVSI
                     LMRLELILIFGMLISLFLFSLFNESQTIVSELTEESDNHIIPWEHVGFFASLIRFPRR
                     YRLPAAIKPSQYAKLGVRLAFRKRDLKYNPAPGILQEVIQLRVRLKELSLPEEGPVSQ
                     "
     gene            2122..3441
                     /locus_tag="VJC18_09910"
     CDS             2122..3441
                     /locus_tag="VJC18_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD45738.1"
                     /translation="MPRDKIKTYGLTLVLLGTVLFVAIILSTIVAYKLFIKHQVLQAV
                     EYKTAVYFVEHSESVREQLLAAIVSIEFDDAKVVEKGDYGVCEIFFDLQLKDDLHERL
                     RVGLVKVSDFWIVYEAELNPYTVAAYPLLDTYRKILFFVKTLEYPDDPSAQIALEAVK
                     GEVLDQDLAHYLTARLLALSNKRDEARQLLDDLAKHVVYSKPAVMFERGTIDFSEKEY
                     ETAIRVYLEIEKEILSRRSNQDRHLKLRNLFRQMPNDPFNAMFSYESILADVYLNLSQ
                     AYVSVGQYEKGLEAAEKAIEQATEIKAKVTLGSARFAQAVILYAMGRYKDANDSFSGV
                     ISDLDNTNLTQKAWSYFHKAEIAVRLGKRSSYIVDYYETAVNLDPFNYPIRKGVIEFL
                     MNRAGPGDLEVALGMALRGVDYGIEPELFSRYAGTLYQRLGIGGNND"
     gene            complement(3379..4209)
                     /locus_tag="VJC18_09915"
     CDS             complement(3379..4209)
                     /locus_tag="VJC18_09915"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HLD45739.1"
                     /translation="MPASLNHELKTAILAALKAGKIISRYYHKSYQVTEKGFDQPVTE
                     ADIAANTIIKKIITKDFPFDGWLSEEDVANPDRFHAKRVWIVDPLDGTKEFIQKNPQF
                     AVSIALVEEQSPLLGVIYNPISRELFWAIKGGGSFFQKQPLSVTKTVLSDKPHLLVSV
                     SEHKRGDWTKFESLFSLIPRGGSAYKMVGIAQGLAEGSFTLCPKNEWDICAGHLIIEE
                     AGGFVCNLDGSPITYNKPITGMDGVIYGCSEEMKKRILSVIVPPYPQPLIKCTSIPRK
                     "
     gene            4325..>4614
                     /locus_tag="VJC18_09920"
     CDS             4325..>4614
                     /locus_tag="VJC18_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45740.1"
                     /translation="MPITFAIALRFLFRSAKTRQIGVFTVVSVLGIAASVFVFLLIDT
                     IINGLTSHLQASLIGFEAPLFVPTDTEGVRPTDQALARFSKEHSEFNLTYYC"
BASE COUNT         1095 a         1037 c         1186 g         1296 t
ORIGIN      
        1 tctcatgttc caaaaggagg tcgctgagag gtgcgtggcc agtcccggca acaaggacta
       61 tggcatcttg agtgtgtggt cacgcctttt gagtcaacca aaaatattgt ttcatctgcc
      121 ccccaccgtt tttaatccaa aaccgaaggt gcaatccgca tttgtccgtt ttgatttgtt
      181 gggggtcgcc tacgaacagg aaaaagactt catttcattt gtcaaaagag tttttggtca
      241 acggcgcaaa aaaatcagca ccattctcaa aaaagaactt cggcatttgg gcacgctgac
      301 gaacgaggtg gcaacactgc tcgatttgcg ggctgaggtg ttgggtattg atagtttaaa
      361 attggttttc gacgctatac gataacatag ccgtaccctt tggattgcca cgttctgcct
      421 gtcggcagaa ctcgcaatga cggcacatca ctgtggagtg agggggccga ttgtttcgtt
      481 atgaaagaga atattgttgg attgatcgat gggccggatc gaaaaagtct tgaatacggt
      541 cttgttgttt cgatcaaacg aggcggagat gatcgtgttg ttatagatcc tcaacacagc
      601 ctgttccggt tcggtattag agaccagaaa gttcttctga ctggccgcgt cggaacgtcc
      661 aaaggcaatc cccagcgcga acgacagaat cagaatgaca gagatcatgc ctttgaagaa
      721 agggctgctg gtactgggca agatgttgta atagagaccg atccacaaaa aagcgatgac
      781 ggccaccatg agtgtgaggc ggttggtgag caagacctgc gtgatgttca cgctgatcag
      841 atccaaagga atgccgaaaa aggccgaata ccctgcttcg tagaacgtga tgaacaaata
      901 agccgctgcc aagggcacca ggaacatggc gagctctttg gtgattttat tgtctgcttt
      961 ttttgaagga aaaacgggtt ctggcgacat aaggctcctt attttgcaca agacacaagc
     1021 gggcattatt ctattctaac cgtcgctaaa gtaaaggaaa aacaatttca ttcgcttgct
     1081 aaatgcgaga cattcattta tccaaagaaa tatgtcgggt gccgattcca tcattattta
     1141 tctttattcc tacttcccag ccatcacgct gggtttgttt ttttggtggc ttgaccgttt
     1201 cgaaagagaa tctcttggtg tggtggtctt tgcttttctc tggggggcca ttggggcagg
     1261 actgctcagt tttttctgga acaccttctt tcatgttctg ctcgaagttt atcaaaaaaa
     1321 cagttttgcc gccaacgagg tcatcattgg tgttgtggtg gccccttttg tcgaagaact
     1381 caccaagggg ttcgttattc tttttttgct gcgctttaag gtcgttgata atgtgactga
     1441 tggcattttg atgggtattc tcattgggct ggggtttgct gccgccgaaa acgtgttcta
     1501 ttgtcaacag gttgtttacc cgaacgatgg ggaattggcc atgtggagca atttgtggtt
     1561 tcgtgaactg cacacgacgc tgttgcatgc ttcggcaaca gctgtttggg gggctgtgat
     1621 tggctattcc cgccagttca ggcgttcgca aaagtggctg gttgtgttgg tggggttcat
     1681 tctggccgtg gtgactcatg gttgttggaa ttttttcgct gtgtatgtca accaaatcca
     1741 gtctcacttc aatctcgtca gcattttgat gcgcctggaa ctgattctaa tttttggcat
     1801 gctgatcagt ctttttctgt tttccctgtt taatgagagc cagacaattg tgagtgaact
     1861 taccgaagag agcgacaatc acatcattcc ttgggagcac gtcggttttt ttgcgtctct
     1921 gatccgcttt ccgcgtcgct atcgtctgcc tgccgcgatc aaaccgtcac agtacgccaa
     1981 attgggtgtg cgtttggcct ttcggaaacg ggatcttaag tataatccgg ctcccggtat
     2041 tttacaggaa gtgatacagt tgcgggtcag gctcaaggaa ttgagcctgc ccgaagaggg
     2101 gcctgtcagt cagtgaggct tatgccaaga gacaagatca agacatacgg actgacgctg
     2161 gttttattgg gcactgtgtt gtttgtggcg atcattctgt cgacgatcgt cgcctataaa
     2221 ctgttcatca aacaccaggt actgcaagcg gtcgaataca aaaccgcggt gtattttgtg
     2281 gaacattccg aaagtgttcg cgagcaattg cttgctgcta ttgtgtccat tgagtttgat
     2341 gatgccaagg tcgtggaaaa aggcgattat ggggtgtgtg aaatattctt cgatttacaa
     2401 ctcaaagatg atctgcacga acggctcagg gtggggttgg tcaaggtttc ggatttctgg
     2461 attgtgtacg aggccgaact gaatccctac actgtcgccg cgtaccccct tctggacacc
     2521 tatcgcaaga ttctgttttt tgtaaaaaca ttggaatacc ccgacgaccc ctcggcacag
     2581 attgcgcttg aagcggtcaa aggcgaagtt ttagatcagg accttgcgca ttatctgacg
     2641 gccaggctgt tggctctttc gaacaaacgc gatgaggcca gacaacttct ggatgatctc
     2701 gcaaagcacg tcgtttacag caagcccgcc gtcatgttcg aaagaggaac catcgatttt
     2761 tccgagaagg aatacgaaac cgctattcgt gtttatctgg aaatcgagaa ggaaatattg
     2821 tcgcgccggt ccaatcagga tcgacatctc aaactgcgca acttgttccg tcaaatgccc
     2881 aacgatccct ttaatgccat gttttcgtac gaaagtatct tggcagatgt ttatcttaac
     2941 ttgtctcaag cctatgtcag tgtgggacaa tacgaaaaag gtttggaagc cgcagagaaa
     3001 gccattgaac aggccaccga gattaaagcg aaagtgaccc tggggtcggc tcgtttcgct
     3061 caggcagtca ttctctacgc catggggcgt tacaaggacg cgaatgactc tttttctggc
     3121 gtgatcagcg atctcgataa caccaacctc acacagaagg cctggtccta ttttcacaag
     3181 gccgagattg ccgttcgttt gggcaagaga agttcttaca ttgttgatta ttacgagact
     3241 gccgtcaatc tggacccgtt caactacccc attcgcaaag gggtcatcga atttctcatg
     3301 aatcgtgcgg gaccgggtga ccttgaagtg gctctgggca tggctcttag aggagttgat
     3361 tacgggatcg aaccagaatt attttctcgg tatgctggta cactttatca gcggttgggg
     3421 atagggggga acaatgactg atagaattct tttcttcatt tcctccgaac agccgtagat
     3481 cacgccgtcc attccggtga tgggtttgtt gtaggtgatg ggggagccat ccagattgca
     3541 gacaaaaccg cccgcctctt cgatgatgag atggcccgcg cagatatccc actcattttt
     3601 cggacagagc gtgaaggacc cctcagccaa tccttgagca atgcccacca ttttgtaggc
     3661 acttccacct cgtggaatga gggagaacag gctttcgaat ttggtccagt ctccgcgttt
     3721 gtgttcactc acgctcacca gcagatgtgg tttatcagac aacacagttt ttgtaacaga
     3781 cagaggttgc ttttgaaaaa acgaaccgcc tcctttgatg gcccagaaca gttcccggga
     3841 gatggggttg taaatgacgc ccagcagggg gctttgctct tcgaccaggg cgatggaaac
     3901 tgcgaattga ggatttttct ggatgaattc tttggtgcca tccagaggat ctacgatcca
     3961 tacccttttg gcatgaaagc ggtcgggatt ggccacgtct tcctccgata accacccatc
     4021 aaagggaaaa tctttagtga tgattttttt gatgatcgta ttcgcggcga tgtcagcttc
     4081 ggtgacaggt tggtcgaaac ccttctcggt tacctgatat gatttgtgat agtaacggga
     4141 aatgattttt cccgccttga gggccgccag gatggcggtt ttgagttcgt gattgaggga
     4201 agcaggcata ggcgtccata tacaaaatca agaaatgaaa caatgaaaca atggtgactt
     4261 cctttcttga ttgcacattg cacaactgct tgattgcttg attgcttgat tgtatacccc
     4321 gcccgtgccg atcacctttg ccatcgccct gcgttttttg tttcgctccg ccaaaacccg
     4381 acagataggc gtttttaccg ttgtgtctgt tttggggata gcggcctcgg ttttcgtgtt
     4441 tttactcatc gataccatca tcaacggttt aacctctcat ttgcaggcat cccttatcgg
     4501 gtttgaggcg ccgctgtttg ttccgaccga caccgaaggt gtcagaccga cagatcaggc
     4561 gttggcccga ttttcaaagg aacattctga atttaacctg acctattatt gcaa
//
LOCUS       DATDUN010000713         1561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_614937, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000713 DATDUN010000000
VERSION     DATDUN010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614937"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VJC18_09925"
     CDS             complement(<1..521)
                     /locus_tag="VJC18_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLD45741.1"
                     /translation="MTTKQTRRNFLKTATVCVGTIAGSKIIGAPYILADKETRAKLRT
                     VVIGCGGQGTGNHTPVAAREQLVALVDPDDKRLAKAMEKAKQTNPKVKMSIKTFNDYR
                     KLFDKMAKEIDVVFIASPNHHHALPALMAMKLGKHVYVEKPLTHTIAEARLMADYARQ
                     YKVATQMGNQGHSG"
     gene            815..>1561
                     /locus_tag="VJC18_09930"
     CDS             815..>1561
                     /locus_tag="VJC18_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45742.1"
                     /translation="MKNSLFNNHVLKICTAVVMLSFLLIGGCFTPIDQLYGSIGINKG
                     EWLVKFNPGNSSLLLEHSLSGARLTGELSFQVETRNKPKKWLIVASRDSITERPALLD
                     ADGNVQGYLSFTGSGDTVHISVIHRAAQSYKGTLLFKGTAQLGRQTFACRTRPNGASR
                     VVQMASGPADSRLNDSLFDVTTDTALRLTGQMVSLLSGRDGVDSPVFDLEMAAVPQDT
                     LYSQLTFQAKRNYYRSQYVPYYKPIDKKRCP"
BASE COUNT          378 a          332 c          390 g          461 t
ORIGIN      
        1 cctgaatggc cctgattgcc catctgggtt gcaactttgt actgccgggc gtagtcggcc
       61 atcagacgtg cttcggcaat ggtatgggtg agcggctttt cgacatagac atgtttgccc
      121 agtttcattg ccatgagggc cggcagggca tgatgatgat tcggtgaggc gatgaaaaca
      181 acgtctattt ccttggccat cttatcgaaa agcttgcgat aatcgttgaa agtcttgatg
      241 ctcatcttga cttttggatt tgtctgtttg gctttttcca ttgcctttgc gagtcttttg
      301 tcgtccggat caaccagtgc gaccagttgt tctcgtgctg caacgggtgt atgatttcca
      361 gttccctgac cgccgcatcc gattaccact gttctcaact tagcgcgggt ttccttgtcg
      421 gcaaggatgt atggcgcgcc aataatcttt gagccggcaa tcgtaccgac acatactgtt
      481 gctgttttaa ggaaatttct acgtgtttgt tttgtggtca tagcggcttc tccgtttcta
      541 gaattgggac aaactgttgg atgtacatat gacaccaatt taaattaaaa tcgaactttt
      601 tttaagcaca tctcaaaatt gaacagctca atcccattct gaggtgggtt tgagaaaaag
      661 ctattgttgt gagtttttgg ggtcaaagga ttacgactgg tcaaaaagta taaaaaatgg
      721 gtcagatcac acatggtcat aatagggacc gtcgcctaca atctaaagaa tctgagttcg
      781 tcaggagcga aacagttaac ttggagatcc aacaatgaag aatagtctgt tcaataacca
      841 tgttttgaaa atctgtacag ctgttgtcat gttatcgttc ctgttgattg gtggatgttt
      901 taccccaatt gatcaattgt acggatcaat cgggataaac aagggtgaat ggctggtaaa
      961 atttaatccg ggtaactcat cgttattact tgaacattct ttgtccggcg ccaggctgac
     1021 aggagaactg tcctttcaag tcgaaacaag gaataagcct aagaagtggt taatagtggc
     1081 atcgagggat tccatcacag aaaggccggc tttactcgat gccgatggca atgtgcaggg
     1141 gtacttatcg ttcacgggtt caggtgatac tgtgcacatt tctgttatcc atcgtgctgc
     1201 ccagtcttat aaaggaacct tgttgttcaa gggtacagcg caacttggca ggcagacttt
     1261 tgcctgtcgt acgcggccga atggtgcttc gcgggtggtc cagatggctt caggtccggc
     1321 cgacagccgg ttaaatgatt ctctgtttga tgttacgaca gatacagcgt tgcggctgac
     1381 tggccagatg gtatcactgc tgtccgggcg tgatggtgtc gactccccgg tttttgattt
     1441 agagatggct gcagtgcctc aagatacgct gtattcccag ctgacatttc aggcgaaacg
     1501 caactactac cgttctcaat atgtgcctta ctacaaacct attgataaaa agagatgtcc
     1561 g
//
LOCUS       DATDUN010000714         2140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_569694, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000714 DATDUN010000000
VERSION     DATDUN010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569694"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VJC18_09935"
     CDS             <1..459
                     /locus_tag="VJC18_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLD45743.1"
                     /translation="PNLVVTRSFSKAFGLASLRCGYLLAQPALCAMVNKIRVGKHVNA
                     LAQVAAAAALDDIPHMLAYVEEVRMAKAWVLRELERRGVPVISTPANFILFRVNEPEK
                     VLSFFENHNIYLRNRSMLPGLTGYLRLTVGSREVMGHFWNVFEKIPSQFI"
     gene            complement(452..931)
                     /locus_tag="VJC18_09940"
     CDS             complement(452..931)
                     /locus_tag="VJC18_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45744.1"
                     /translation="MAQFLYTKTVKPNLGNTLSGFSPSRKKGSGCGGCLILFFTVFVL
                     LLLVSAGGYWYLIQEENDEPIGIAAIYLVKNKSVHDSVVRDIQGNALLPQDEKLAVTL
                     TYDIFLEKYDSLPTKQQTVIKKNLGRLAKKIIPNRQLLATEPLPEEVKNIIAVLLVK"
     gene            complement(953..1588)
                     /locus_tag="VJC18_09945"
     CDS             complement(953..1588)
                     /locus_tag="VJC18_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45745.1"
                     /translation="MTEATTQSPQNPLPPYIPQDPPKKKGSGCGCFLAGCLVLIALIL
                     LPIIGGTIYVYSWNASDWGSHIVNIISHPQLVSSFKKAINDSPSFTVEQKQSLTKAYD
                     SFLTVHKSLSEEQKEKLQKDIYITLKKIIADPEQFNQSPPPELIEIFTLLGMDKPNSP
                     AAENSPNNPKTDQPLAIPQDPYSFVNSAVVPVTEQTTKPAEEQVDKQGPLQ"
     gene            complement(1597..>2140)
                     /gene="fsa"
                     /locus_tag="VJC18_09950"
     CDS             complement(1597..>2140)
                     /gene="fsa"
                     /locus_tag="VJC18_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HLD45746.1"
                     /translation="TGRKFDDVIKDICAIVDGPISAETISLKPEEMFVEACKLSKIHP
                     NIVVKIPLTTEGLKVTKKCTTEGIKTNVTLCFSPNQALLAAKAGATYISPFVGRLDDI
                     THVGMDLIEQICTIYRNYEFTTKIIVASVRNPLHVYEAAMIGADVCTIPFGVIKQLAA
                     HPLTELGIEKFLKDWEKVPK"
BASE COUNT          456 a          491 c          594 g          599 t
ORIGIN      
        1 cccaatctcg tggtcacgcg ttctttttcc aaggcctttg ggttggcttc cctgcgttgc
       61 gggtacctgc tggcccagcc ggcactgtgt gccatggtca acaagattcg tgttgggaag
      121 catgtcaatg ccttggcgca ggtggccgcc gccgccgcgt tagacgatat cccgcacatg
      181 ctggcctatg ttgaagaagt gaggatggcc aaggcctggg tcctgcgcga attggaaaga
      241 cggggcgttc ctgttatcag cacccctgcc aattttattt tattccgggt gaacgaaccc
      301 gagaaggtcc tttccttttt tgagaaccac aatatctacc tccgaaatcg ctccatgttg
      361 ccgggactga cgggttatct tcgcctgact gtcgggagcc gtgaggtgat ggggcatttt
      421 tggaacgtct tcgaaaaaat ccccagtcag tttatttgac caacaacacc gcaatgatgt
      481 ttttgacttc ttcgggcaga ggttctgtgg ccaagagctg tcggttgggg atgatttttt
      541 ttgcgagacg gcccagattt ttcttgatca ctgtctgttg cttggtgggg agtgaatcat
      601 atttttccaa aaaaatatcg taagtcagcg tcacagccaa tttttcgtcc tgaggcaaca
      661 gcgcgttgcc ctgaatatct ctcaccacgc tatcatgcac tgacttgttt ttgaccagat
      721 agatggccgc gattccgata ggctcgtcgt tctcttcctg tatgaggtac cagtaaccac
      781 cggccgagac caagaggagt aagacgaaga ctgtgaaaaa cagaatcaaa cacccaccac
      841 agcccgaccc cttttttcgt gacggggaaa aacccgagag cgtgttgccg agattgggtt
      901 tgacagtttt tgtgtacaga aattgtgcca tagcgtcctt aaaaagccat tgtcactgaa
      961 ggggtccctg cttgtcgact tgttcttctg ccggttttgt tgtctgctcc gttacgggaa
     1021 ccaccgcgct attcacaaaa ctgtagggat cttgtgggat ggccaggggt tgatctgtct
     1081 tcggattgtt cggactgttc tctgctgcag gactgtttgg tttgtccatg cccagcagtg
     1141 tgaagatttc gattagttcg ggaggtggtg actgattaaa ctgttcgggg tctgcaatga
     1201 tttttttgag tgtgatgtaa atgtctttct gaagtttttc cttctgttcc tccgagagtg
     1261 atttgtgcac ggtcagaaac gaatcgtagg ccttggtgag ggattgtttc tgttccaccg
     1321 taaagctggg ggaatcgttg atggcctttt tgaaactgga gaccaattgg ggatggctga
     1381 tgatgttgac gatgtgtgag ccccaatcgg aagcgttcca ggaatacacg tagatggtgc
     1441 ctccgatgat gggcaacaag atgagggcaa tgaggaccag acagcccgcc aaaaaacaac
     1501 cacagccgga gccctttttt ttgggggggt cctggggaat gtaaggcggt aagggatttt
     1561 gtggggattg tgttgtggct tcagtcatgg gtctccttat ttgggaacct tttcccagtc
     1621 ctttaaaaat ttttcaatcc ccagttctgt gagggggtga gcggccagtt gcttgatcac
     1681 gccaaagggg atcgtgcaca catcggcgcc aatcatggcc gcctcataca catgcagtgg
     1741 gttgcgcaca ctggccacga tgattttggt ggtaaattcg taatttcgat aaatggtgca
     1801 gatttgttcg atgaggtcca tgcccacgtg cgtgatatcg tccagtcgtc ccacaaaggg
     1861 gctgatgtaa gtggcaccgg cctttgccgc caggagggcc tgatttggcg aaaaacacaa
     1921 tgtcacgttg gttttgatgc cctcagtggt gcattttttt gtgactttga gcccctcagt
     1981 cgtgaggggg atcttgacga cgatattggg atgaatcttg gacagtttgc acgcctcaac
     2041 aaacatctct tcgggtttca gggaaatggt ttctgccgag atagggccat ccacgatggc
     2101 gcaaatgtcc ttgatcacat cgtcaaattt gcgacccgtt
//
LOCUS       DATDUN010000715         1045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_78197, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000715 DATDUN010000000
VERSION     DATDUN010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78197"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1045
                     /locus_tag="VJC18_09955"
     CDS             <1..>1045
                     /locus_tag="VJC18_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HLD45747.1"
                     /translation="GTYNYDHQVTVDKRVAIVGESSNNTTINISGQHGFLVDQIMLPQ
                     AVKFENLHIRGSGNVSTIQTRNSRVLIKDTKITNSLGHGLFISGGQVEVKNNLFYGIA
                     LHSIFVAKAPLVPPSPMTQVIIFNNIITASHQTGLFIQNNDPNTIITVARNLFADNRI
                     GALHTLANTPQNGRNTASLFSFNNTFVGNQGPVIGADDGSSFNKNNIYAFNDGPDMEP
                     NGLQLGMQQREYNLTFGNTPDNLRFDPNTLNADPKFPATDNGVSSGLTENTLTDALKD
                     WPVDSLRGKFLIANEFKNVYYISGNTSNTITILVNHPDHELTDMTAVGQPYMIIDGFV
                     LAPDSPAINTGTPA"
BASE COUNT          279 a          324 c          224 g          218 t
ORIGIN      
        1 cgggaactta caactacgat catcaggtca cggttgacaa aagagtggcc atcgttggcg
       61 agagtagcaa caacacgacc atcaacatca gcggacagca cggttttctg gtagatcaga
      121 tcatgttgcc tcaggccgtg aaattcgaaa atctgcacat tcgcgggagt ggtaatgtga
      181 gcacgatcca gacccgcaat tctcgcgtcc tcatcaagga caccaaaatc accaactcac
      241 ttggtcacgg tctcttcatc tccggcggac aggtggaggt gaagaacaac cttttttacg
      301 gtatcgcgct ccattccata ttcgtcgcca aggcgccact cgtgccacca tcgccgatga
      361 cacaggtcat catcttcaac aacatcatta ccgcctcgca tcaaaccggc ctctttatac
      421 aaaacaacga tcccaacacg atcatcacgg tagcccgcaa cctttttgcg gacaacagga
      481 taggcgcctt gcacaccctc gcaaacacgc cgcagaatgg acgcaacacc gcatcgctct
      541 tttcgttcaa caacaccttc gtcggcaatc agggtcccgt catcggggcc gatgatgggt
      601 cctcgttcaa taaaaacaac atctatgcct ttaacgatgg acccgatatg gaacccaacg
      661 ggttgcagtt aggcatgcaa cagcgggaat acaacctcac ctttggcaac acaccagaca
      721 acctgaggtt tgatcccaac accttaaacg ccgatcccaa gtttcccgcg acggacaacg
      781 gtgtcagttc cggtttgacc gaaaacacac tcaccgacgc gctcaaggac tggccggtcg
      841 attcgctcag gggcaagttc ctcatcgcca atgagttcaa aaatgtttat tacatctctg
      901 gcaacaccag caatacgatc acgatcctcg tcaatcatcc cgatcacgaa ctcactgaca
      961 tgactgccgt gggtcaaccc tacatgatca tcgatggctt tgttctggca cccgattcgc
     1021 ccgcgatcaa taccggcacc cctgc
//
LOCUS       DATDUN010000716         1024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_38076, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000716 DATDUN010000000
VERSION     DATDUN010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38076"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VJC18_09960"
     CDS             complement(<1..500)
                     /locus_tag="VJC18_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45748.1"
                     /translation="MKKNIPFFLFLLIVCWCPLCLAQTPESKTDYLGYYKTSHSYQRV
                     IEAGATVINYPEDHSFAVLWVPPEVTQGRIMVLLHGSQGTPYEEIKDELAMAQKYKYL
                     LVGIQWHDRQTDRYYPASQVYRIIDKTLQYVKKKYSNDLSRVAFSGFSRGAAVSYELA
                     YFDLTH"
     gene            complement(835..>1024)
                     /locus_tag="VJC18_09965"
     CDS             complement(835..>1024)
                     /locus_tag="VJC18_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45749.1"
                     /translation="FLLRRCCPKKNSLCPASQIMGKDQNDLEGGCSIQITHFYFMSHT
                     PQWQSLSGNLSVAPAGDY"
BASE COUNT          287 a          237 c          238 g          262 t
ORIGIN      
        1 ctgtgggtca aatcaaaata ggccagttca tacgagacgg ccgcaccgcg actgaaaccc
       61 gaaaaggcca cacggctcaa atcgtttgaa tatttctttt taacatactg cagggtcttg
      121 tctatgatgc gatacacctg cgacgctgga taatagcggt cggtctggcg atcatgccac
      181 tgaatgccca ccagcagata cttgtatttt tgggccatgg ccagttcgtc cttgatttcc
      241 tcataggggg tgccctgtga cccgtgcagg aggaccatga tgcgtccttg tgtcacctcc
      301 gggggaaccc acaagacggc aaagctgtga tcctctggat aattgatgac cgtggcgcct
      361 gcttcaatca cacgctgata gctgtgagag gttttgtaat atcctagata atctgttttg
      421 gactcagggg tctgagccag acacagaggg caccaacaaa caatcaacaa aaacaggaaa
      481 aagggaatgt tttttttcat tggtctttct ttggcaacat ttccccaccc tgacaatgaa
      541 aaaaaatatc tccccatcgg ggccatcaaa agagatagct catttcactt tgaccggtca
      601 aactgataac agcataggaa acaactggca ggcagcaaat tgtcagaaga agatactcga
      661 gagaatatgc agatagcgat ctttaatttt ctactgcatg acaaggacag aacgacctca
      721 aaaatatcat atgaaggatt ctcttctttg ttcgatgaca tattccttgt tgacatggca
      781 tctaggtgac cggatcaatg tgtcttgatg gcgaggaata ccctactttt catttcaata
      841 gtcacccgct ggggccactg agagattgcc actgagagat tgccactgag gggtgtgcga
      901 cataaaataa aagtgggtga tttgaattga gcagcccccc tccaaatcat tctgatcttt
      961 acccataatc tgacttgctg ggcacaggga gttctttttg gggcaacatc gtcgcaacaa
     1021 aaat
//
LOCUS       DATDUN010000717         2181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_114682, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000717 DATDUN010000000
VERSION     DATDUN010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114682"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VJC18_09970"
     CDS             complement(<1..1203)
                     /locus_tag="VJC18_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HLD45750.1"
                     /translation="MKKFSKLLLVVLVMLGLQACATGADVGQQGTGTANVATGDVDVG
                     IDQSFTVTFSAAVDPDTVTTDSFFVVPVSDPEPTLIKLAIFEAGVCDPDHAIDATVTC
                     ATNTECTLTPTGVLDPAAEYAICLTPAIRFADENMYGYFDGLTETFVTEASPAEPEPA
                     PDATYTVGGTMTGVLNPAVLQNNDGDDLTVEEDGAFVFATQLEDGASYSVTVATPPGE
                     MNCTIENGTGTIAGANVTNVVITCVDMEVTDIPVMEPAGGAYDAVQEVTITSTTEGAT
                     IYYTADGSYPSTSSTVYTGPVEIGEGETVLKAIVRKAGMVGNVAASETYVITLEEPEA
                     VKKIFVTDATYNGNLGGVAGADALCEADANKPATGTYKALIVDGVARVACTTANCGGG
                     AGENIDWVL"
     gene            complement(1260..>2181)
                     /locus_tag="VJC18_09975"
     CDS             complement(1260..>2181)
                     /locus_tag="VJC18_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HLD45751.1"
                     /translation="SNIFFKGGLVKKLFLILFVLFFIVVTNAFAKDISLDAVSFHPAA
                     DGSGFKGLWQSSVLQNDHWIFGSDLSYAYRPFQLMNNGNRQDGIVDQMISQHFFASVG
                     LIDQKFELGIELPIVWYADYKNPNVAGSVFESESGLGDARLNFKLCLWNPENHKLGIA
                     WVPYITLPTGDSDHFLGSGVVALGNLLVIDGNISDKFSFNINAGILARDVFYFRDIEK
                     THQLLGGLGLAYEFTEKLTARLETNIKTRLSGLFEESVESPVEAMASLAYRWKDTGFL
                     LNAGVGTGIVYGSEAPLFRGVLGLTYEGAK"
BASE COUNT          562 a          621 c          484 g          514 t
ORIGIN      
        1 caaaacccag tcaatatttt cacccgcacc accaccacaa ttggctgtcg tgcaggccac
       61 gcgagccaca ccatccacaa tcaacgcctt gtaagtgcct gttgccggtt tgttggcatc
      121 ggcctcgcaa agggcatcag cgcccgctac gccacccaaa ttaccgttgt acgttgcgtc
      181 ggtcacaaag atttttttga ccgcctcggg ctcttccagc gtgatcacat acgtttcgct
      241 cgccgcaaca ttgccgacca ttcccgcttt gcggacaatc gccttgagaa ctgtttcccc
      301 ctcaccaatc tcaactggcc ccgtataaac agtgctggag gttgaaggat aagaaccatc
      361 agctgtgtaa taaatcgttg ccccttctgt cgttgatgtg atcgtcactt cctgcacggc
      421 atcgtacgcg ccacccgctg gttccataac gggaatatca gtcacttcca tatcgacgca
      481 cgtaatcaca acattggtga cattggcacc agcgatcgtt cctgtcccat tctcaattgt
      541 acaattcatt tcacccgggg gtgttgcgac tgttacagaa taagaggccc cgtcttcaag
      601 ctgtgtcgca aaaacgaacg ctccatcctc ctcgacagta agatcgtcgc catcattatt
      661 ttgtaagact gcgggattga gcacccccgt catcgtacca cccacggtgt atgttgcatc
      721 aggtgctggc tcgggttctg ccggagatgc ctctgtcaca aatgtttctg tcaggccatc
      781 aaaatacccg tacatgtttt cgtcggcaaa acggatcgca ggagtcaaac aaatcgcata
      841 ctctgccgca gggtctaaca ctcccgtggg tgtgagtgtg cattctgtgt ttgtggcaca
      901 tgtgaccgtt gcgtcgatcg cgtgatcagg gtcacaaaca cccgcctcaa aaatggccaa
      961 cttgatgagc gtgggttctg ggtcagagac tggcacaaca aaaaaactgt cggtggtcac
     1021 tgtgtctggg tcgacggcgg cactgaacgt caccgtaaaa ctctgatcga tgcccacgtc
     1081 cacatcgccg gtcgcgacgt tcgctgtccc agtgccctgt tgccccacgt cggcaccggt
     1141 ggcgcaggct tggagaccaa gcatgaccaa aacaaccaac aacaattttg aaaacttctt
     1201 catcataaaa cccccacgat tcacacgcga tacgcgatac gctttatgcg tgcatcaccc
     1261 tattttgccc cctcgtacgt cagccccaga acgcccctaa acagcggcgc ttccgaaccg
     1321 taaacaatgc ctgttcccac acccgcgttc agcaaaaaac ctgtgtcttt ccaccggtac
     1381 gcaagactgg ccatggcctc gacgggcgat tcaacgcttt cttcaaacaa acccgacaag
     1441 cgtgttttga tgttggtttc cagccgcgct gtgagtttct ctgtgaattc atatgccaaa
     1501 cccaaaccgc ccaaaagctg gtgtgttttt tcgatgtccc gaaaataaaa aacatctctg
     1561 gccagaatcc cggcattgat gttgaatgaa aacttgtctg agatgttgcc atcaatcacc
     1621 aaaagattgc ccagcgccac aacgccactg cccaaaaaat gatcgctgtc tccggtgggc
     1681 agggttatgt agggaaccca cgcgataccc agtttgtgat tttcgggatt ccacaggcaa
     1741 agcttgaaat tgagccgggc atcgcccaaa cctgattcgc tttcaaatac tgaacctgcc
     1801 acgttgggat ttttgtaatc agcgtaccaa acaataggaa gctcaatgcc cagttcaaat
     1861 ttttgatcga tgagtccaac cgaggcaaag aaatgttgtg agatcatctg gtcaacgata
     1921 ccatcctgtc ggttgccgtt attcatgagc tggaatgggc gataggcgta agacaaatca
     1981 gaaccaaaaa tccaatggtc gttctgtaaa acagaacttt gccacaaccc cttaaatccg
     2041 ctcccatcag ctgcgggatg aaatgacacg gcatccagac tgatatcttt ggcaaacgcg
     2101 ttcgtgacga caataaaaaa taaaacaaac aggatcaaaa agagcttttt cacgagacct
     2161 cccttaaaaa atatattgga a
//
LOCUS       DATDUN010000718         3390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_322198, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000718 DATDUN010000000
VERSION     DATDUN010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322198"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VJC18_09980"
     CDS             complement(<1..1158)
                     /locus_tag="VJC18_09980"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLD45752.1"
                     /translation="MRKRILFFKTLTDRIVRNNRGRFLLVLSAFCGLIVFNLLLSDYL
                     VYPGKVVFSGLFGFIIVASQLGLAYLFGVNRGIWKYSSISDLILIVKVAAVSFALNIV
                     SMLVTGLGHDALRILLVDHMLFVLSFGGLRLLVRLYRNVGPVPSTLKRVLIVGAGDGG
                     DIACRRVKHDTTQYEVVGFLDDDPWKKNHEIHGVKVLGATHQIVEIVQANEVNEVVIA
                     IPSAGPALLRSLAEKCFEAKAQVKIMPSLSELSENSATFEIRNLKLEDLLAREPIIED
                     LSSVRKTYVNKRVLVTGAAGSIGEELVKQVAQMGPAIVYLLDQAESPLYFLFRECQVK
                     FPNVEFVPMLCDITDHWALGRFFKQAQPQIVLHAAAYKHVPILEDNIYQAIS"
     gene            complement(1249..2376)
                     /locus_tag="VJC18_09985"
     CDS             complement(1249..2376)
                     /locus_tag="VJC18_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HLD45753.1"
                     /translation="MTENVGTTAKGHSGETNILDILRVVNKNKLMIFLALLIVSVVAV
                     IGSGLMDKQYRSTAVIFPVSANESMGGFSSLLAQFGGLASLAGVSAGTSSTTKLVMIL
                     KSRSVAEAVIEKEQLMKVLFMDYWDNTTNQWMTTTPEEMPTLQKAVTLLMSGMVSVIE
                     DIKEGSISVSVATTDPQLSARIANAYLRELQVFLGKQTLTSAKRNRIFLEQQMAQNKE
                     DRLKNGMEINDFSRNVGTGVLPLLPAVSTTVQTVSGVDAVDVEKLLKEKAENERKIAE
                     SKSNGKVPLSVLLENLMAQHQLLLQVQMTLAGQYEMAKIQEAKEDISFTVIDPAIPPE
                     EKFAPKRAQICIISFMGALFISVFVAFFREYITRMKALEQA"
     gene            complement(2422..>3390)
                     /locus_tag="VJC18_09990"
     CDS             complement(2422..>3390)
                     /locus_tag="VJC18_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HLD45754.1"
                     /translation="LGVGPGDEVVVTPRSFIASVGCVITVGAKPVFADVDPDSGNITA
                     DSIAKVLTKKTRAIIPVHLAGWPCEMDAIITLAKDHGLFVIEDCAQAHGAEYNGKKVG
                     SIGHVGAWSFCQDKIMTTGGEGGMLTTNDGEVWNYAWSYKDHGKSYQAMNNGEEGNCF
                     KWVHHSIGTNWRMIEMQAAIGRIQLKKLDQWHLARRANAQAILAAARQCPALRVPEVP
                     SHIEHAWYKCYVYVRPEALSSGWSRDRIVAEINKQGVPCYTGSCSEIYLEKAFDVTGL
                     RPEKRLALAKQLGETSVMFVVHPTLTQNELHRTCSTLTDVVSKATR"
BASE COUNT          957 a          880 c          662 g          891 t
ORIGIN      
        1 agaaattgcc tgataaatat tatcttctaa aattggcaca tgtttataag ccgctgcgtg
       61 caaaacgatt tgtggctgtg cctgtttaaa aaaacgaccc agcgcccagt gatcagtaat
      121 gtcacaaagc atgggcacaa attccacatt gggaaatttt acctgacatt cgcggaacaa
      181 aaaatacaaa ggtgattctg cctgatctag caaatacaca atcgctgggc ccatttgcgc
      241 cacctgcttt accaattctt caccaatgga gcctgcggcc ccagtcacca gcacgcgttt
      301 attaacgtaa gttttgcgaa ctgatgagag atcttcgatg atgggttctc tggccaacaa
      361 atcttccagt ttaagattcc tgatttcaaa cgtagcagaa ttctcagaaa gttctgaaag
      421 gctgggcatg attttgactt gggctttggc ttcaaaacat ttttcagcca aacttctgag
      481 cagtgctggc ccagcagagg gaatggcaat cacaacctcg ttcacctcgt tggcttggac
      541 aatctccact atctgatggg ttgcccccaa aaccttgaca ccatgaatct catgattttt
      601 cttccaagga tcgtcatcca aaaagcccac tacctcatac tgtgtggtgt cgtgtttgac
      661 gcgccgacag gcaatgtcac caccgtcacc ggcccccaca atcagtaccc ttttaagtgt
      721 tgagggcacg gggcccacgt tcctgtacaa cctaaccaat aaacgcaaac caccaaaact
      781 caaaacaaac aacatgtggt ccaccagcaa aatgcgcaag gcatcatgcc ccaagcccgt
      841 cacgagcatg ctgacaatat tcaatgcgaa actgaccgcc gccactttga cgatcagtat
      901 cagatcggaa atgctcgaat acttccaaat gccgcgattg acaccaaaaa ggtaagccag
      961 ccccaactgg gacgccacaa tgatgaaccc aaaaaggcca gaaaacacta ccttgccggg
     1021 atacaccaga taatctgaaa gcagaagatt gaagacgatc aagccacaaa aggcagacaa
     1081 gaccaataaa aaccggcccc tattgtttct cacaattctg tcggtcaggg ttttaaaaaa
     1141 taagattcgt tttctcatga gtgcctcaca gagcgggcgg tataatcata agcgaaaagg
     1201 gatgcaatca aaagatagat tggagcatca aatagtcaga tgcggtggtt aagcttgctc
     1261 caaagctttc atgcgggtaa tgtattcacg aaaaaaggcc acaaaaacag agataaacaa
     1321 ggcccccatg aaagaaatga tgcaaatctg cgctcttttg ggtgcaaatt tttcctcggg
     1381 agggatcgcg ggatcaatga ctgtaaacga aatatcttct ttggcctcct gtattttggc
     1441 catttcgtat tgtccggcaa gcgtcatctg cacctgcaag agcagttggt gttgtgccat
     1501 caagttttcc aaaagaacag ataatggcac cttaccattt gactttgatt cagcaatttt
     1561 gcgttcgttt tctgcctttt ctttgagaag tttttcaaca tcaacagcat caacaccact
     1621 cacagtctgt actgtcgtag acacagctgg caataacggc aagacgccag tcccaacgtt
     1681 tcttgaaaaa tcattgatct ccatcccgtt tttcaagcgg tcttccttgt tttgcgccat
     1741 ttgttgttcc aagaatatcc ggtttcgctt ggcagaagtg agtgtctgtt ttcccaagaa
     1801 aacctgaagt tcccgcaaat aggcgtttgc aattctggcc gaaagctgtg gatccgtggt
     1861 tgcaacactt acagaaatcg agccctcttt tatatcctct attaccgaaa ccattccact
     1921 cattaatagt gtcacggctt tctgtaatgt tggcatttct tctggcgttg tggtcatcca
     1981 ttgattggtc gtgttatccc aataatccat gaacaacacc ttcattagct gttctttttc
     2041 aatcacggcc tccgcgactg aacgactttt taatatcatt actaatttag tggtactact
     2101 ggttccagca ctcacccctg caagagatgc caggccacca aattgtgcta aaagagaaga
     2161 aaagcctccc atggactcat tagcacttac aggaaaaata accgctgtcg atcgatattg
     2221 tttgtccatc aggccactac caataaccgc cacaacagac acaatgagaa gggcaagaaa
     2281 gatcatgagc ttgttcttgt tgactaccct caaaatatcc aggatatttg tttctccact
     2341 atgccccttg gcagttgtac caacattttc tgtcataatg atcagtgcct caattttagg
     2401 atattagtca aacatcagcc actagcgtgt agcctttgat acaacatcag tcaatgtaga
     2461 acaagtccta tgtaactcat tttgcgttaa ggttggatga acaacaaaca tcacactggt
     2521 ttcgccaagc tgttttgcca gtgcaagacg tttttctggc cgcagccctg taacgtcaaa
     2581 agctttttcc agataaatct ctgaacacga acccgtgtaa cagggaaccc cctgcttgtt
     2641 gatttcggct acgatacgat ctcgtgacca ccccgaagac aatgcttcgg gcctcacata
     2701 cacataacac ttataccaag catgttctat atgagaagga acttcaggaa ctctcaatgc
     2761 aggacattgt cttgccgcgg ccaagattgc ctgtgcattt gctctgcgcg ctaaatgcca
     2821 ctgatctagt ttttttaatt gaattcttcc aatcgccgcc tgcatttcaa tcatgcgcca
     2881 gttggtacca atggagtgat gcacccactt aaaacaattg ccttcttcac cattattcat
     2941 cgcttgatac gatttcccat gatccttata agaccaggca taattccaaa cctctccatc
     3001 gtttgtcgtg agcatccctc cctcaccacc tgtggtcatg attttatcct gacaaaacga
     3061 ccaagcaccc acatggccga tacttcccac ttttttccca ttgtactctg ccccatgggc
     3121 ctgagcacaa tcctcgatca caaaaagccc gtggtctttc gcaagtgtta tgattgcatc
     3181 catctcacaa ggccaaccag caagatgcac agggataatc gccctggttt tttttgttaa
     3241 taccttcgca attgaatcgg ctgtgatgtt gccactatcc ggatcaacat cagcaaatac
     3301 aggcttcgca ccgactgtga tcacacaacc tacggaggca ataaaactac gaggcgtcac
     3361 caccacctca tcaccgggcc ccactcccag
//
LOCUS       DATDUN010000719         1544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_288996, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000719 DATDUN010000000
VERSION     DATDUN010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288996"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VJC18_09995"
     CDS             <1..792
                     /locus_tag="VJC18_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45755.1"
                     /translation="EADGDGNVLICGSNNEVDPGEFLYRYDVAEDTYTSLADDDIVTA
                     AIGGSSISACGSVWAIGDSIVFDSNTGNQVLYSTDNGATWGYEYLSTQLAQFTGDDTT
                     FYGAGGVIADGPAFWTADTTFTDLTMDDDIDSNKQEARAGVTITTDDSTLIMGGANSG
                     DTDLWIIYSENGGTNWSNATITTTHNFSFIRGLDCVSATVCLAVGDYYPASDGGFILI
                     SEDAGHTWTDVEATSELPTLYSITSSGNTAWLAGDGGNVYTLDVN"
     gene            complement(911..>1544)
                     /gene="nagZ"
                     /locus_tag="VJC18_10000"
     CDS             complement(911..>1544)
                     /gene="nagZ"
                     /locus_tag="VJC18_10000"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HLD45756.1"
                     /translation="VKDAGFNLNFAPVVDVDLNPQSPVIGDRSFSDDPEVVATLSAQI
                     VRGLWDEGVQACLKHFPGHGATEADSHFELPVDRRSLDDIRKTDLIPYQKLIAQNLAP
                     SVMTAHVLYPEIDPERPATLSKKIIHDILRGEMGYEGIIFSDDLRMKAIHDHHDLADA
                     SELFFNIGGDVVLICNEAELILDVVEKLQTRDLNLLQQRQRLCEFLSFCK"
BASE COUNT          381 a          405 c          396 g          362 t
ORIGIN      
        1 gaggccgatg gcgacgggaa tgtcctgatc tgcggttcca acaacgaggt tgaccccggt
       61 gagttccttt atcgctacga tgtcgctgag gacacgtaca catcgcttgc cgatgacgac
      121 atcgtcacag cggccattgg aggctcatca atttccgcgt gcggtagcgt ttgggctatt
      181 ggtgattcca ttgtttttga cagcaacacg ggcaatcagg ttctctattc gacagacaat
      241 ggagcaacgt ggggttatga atacctgagc acacagctcg cccagtttac aggcgacgac
      301 acgacctttt acggtgctgg tggtgtgata gcagatggac ctgccttctg gactgctgac
      361 accaccttta cggacctgac gatggatgac gatatcgact ccaacaaaca agaggcccgc
      421 gcgggagtca ccatcacaac tgatgattca acgttgatca tgggtggtgc caattcaggt
      481 gacaccgacc tctggatcat ctacagtgag aatggcggca ccaactggag caacgccacg
      541 atcaccacca cacacaactt ctctttcatc cgtggtctgg attgcgtgag cgccactgtc
      601 tgcctggcag tgggtgatta ttaccccgcc tcggatggcg gattcattct gatctctgag
      661 gacgctggcc acacctggac ggatgtggag gctaccagtg aattgcccac gctctattcc
      721 atcacaagct ctggcaacac cgcttggttg gcgggagatg gtggcaatgt ctacacactg
      781 gatgtgaact gacgatcgat aataaattgt tttttaaaac ccccgcacag acctctctct
      841 gttcgggggt tttttttcga tgaatgggaa agaatttgag acagggattg aaccgagagt
      901 aaaacgtgta ttacttacag aacgaaagaa attcacaaag acgttggcgc tgttgtagca
      961 aattgagatc cctcgtttgc aacttttcaa ccacatccaa aatcagctcg gcctcattgc
     1021 aaatgagcac cacgtcacca ccaatgttga aaaacaattc actcgcgtca gccagatcgt
     1081 gatgatcgtg aatggccttc atgcgcaggt catcagaaaa aatgatgccc tcgtacccca
     1141 tttccccacg caggatgtcg tgaatgattt tttttgacag cgtggccggc cgttcaggat
     1201 cgatctccgg atacaagaca tgcgcggtca taacggaggg tgccagattc tgtgcgatca
     1261 atttttgata gggaatcaga tctgttttgc ggatgtcatc gagcgagcgg cggtcaacag
     1321 gcagttcaaa atgactgtcc gcctcagtgg cgccatgccc gggaaaatgt ttgagacagg
     1381 cctgcacccc ttcgtcccaa agccccctga caatctgcgc gctcaatgtg gccaccactt
     1441 ctggatcatc agaaaaagaa cgatcgccaa tcacgggact ttgcgggttg agatccacat
     1501 ccaccacggg cgcgaagttc agattaaacc cagcgtcttt gacc
//
LOCUS       DATDUN010000720         3805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_322339, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000720 DATDUN010000000
VERSION     DATDUN010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322339"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VJC18_10005"
     CDS             complement(<1..502)
                     /locus_tag="VJC18_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45757.1"
                     /translation="MSDLSVRPHQADDVCSEEYGRLQVIDETPSGDPSLRLKLQETQQ
                     EYYCHMQEMLGFVMDPLTIGIYGRKNWPTATAGFPYGITNYSVQRKEIFSTQQDIKKD
                     IYVPFFVKFLSGRLTQQQLDQMANAFHVEPRDNIKDVIAQISEEDAYDQYATHYGLMN
                     EMGLHAV"
     gene            680..3685
                     /locus_tag="VJC18_10010"
     CDS             680..3685
                     /locus_tag="VJC18_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45758.1"
                     /translation="MPIINLPSPQGFFSTASGTSESPVLLLGRDINPQTVPDLRSGIL
                     QSVEGDRQAWARGEALGEVGVREDWYASADQVLEQLPVCGQWSVFKYEQTASEVPATL
                     CAESTPHDVAQLGTLIDWLVRLSQQSGEPIRREVVQEVLLVCFTSRVVSVDIEEESSE
                     AFIGEETPEPQDLRATLDFLYDCLRDGELQNHLNPHHPYDNLRILLGSIHRLHRLICL
                     NAPDCLMLGDIRILSGRLMSCLKPEAALAFTRVRYAPLVQSIERHPALAPFAAQAKKI
                     ACLIEMSCYQKSAVRFESITTMSHLFGLCLPEHQPALADALLEVINKTPAEEVSRLIE
                     FMGCVEEDVVGEATVIVNLNTILRKVTGRRVLAIQNVVFLQEVIRRTHGCVQRQQGLM
                     KDPDSELSSGFEQLEDPLSLLDGLRQILSYHPTPEVLRAMMTADAQSGTQNHLIWVLK
                     NIPKGSQTRDARRFLADYLLLRTRLKLFLARAQRGDWTDDNIGKLRELYAWEHDPALR
                     GEIRTPEDAASDRDLIAEMKDEVALLGYDDYKQFRDYGFDVTLPLETVAKLQEIDSFF
                     DDMLISINRTQTDSAKRAETMLAFIEFCRVVTNLGNLLPGLAFGEIIEGMRAQCAQKR
                     RREFMDAAEIKKITETIKLAALDDFFAKSSLPADTQSKIREQMQNPESPLSEPELINE
                     IVAYYHFQGPGGREAVNEYLRMIANQGRHVARKGVLPVEREVTLMRRQTSRREGFLTV
                     YRSILEHLMVEGSAEIAEADLRTVIMDVEVCLRDGRRISHDKLFEWQQRLQECMSQQP
                     NGQITEVLHDLEQIRTLQENRNQAPGTTYDFFITDDPVVIARAGEEPLRTCQRISSPG
                     AYNREGQPLWRAMHPHVSLAGLKDPHTGKVVARVLLEYVRPGIMLVERHYLAGGISME
                     EFDYALLSELARQRRLRHVVFADNRGLPFTAETVLVGEIPEYFYHDTFYSHAEFRAIR
                     LDDKDTRMALQTHGVLFMRPVKKRKKITG"
BASE COUNT          958 a          833 c         1025 g          989 t
ORIGIN      
        1 caacggcgtg cagtcccatc tcgttcatca gaccatagtg tgtggcatac tgatcataag
       61 catcttcttc ggaaatttgt gcaatgacat ccttgatatt gtctctgggt tcaacgtgaa
      121 atgcgttggc catctgatcc agttgttgct gggtcaatcg accagacagg aatttgacaa
      181 aaaaaggcac ataaatatct tttttgatat cttgttgtgt ggagaagatt tcctttcttt
      241 gcacggaata attcgtgatc ccataaggaa atcccgctgt cgcggtcggc cagttttttc
      301 gtccataaat accgatggtc aggggatcca tcacaaaacc gagcatttcc tgcatgtggc
      361 aataatactc ctgctgggtt tcctgcaatt tgagtctcaa cgaagggtct cccgacgggg
      421 tttcgtcaat gacttggagt cgcccatatt cttcggaaca cacatcatct gcttggtgag
      481 gcctgacgga cagatcgctc ataaacatct cctatttgcg aaactactgt tcactgcttc
      541 gtcaacccac ataaaaagtt gcttgtctca aaaaatttct gtccacaatt gtggacggaa
      601 atttggcgac acacacaact ttcatgccta tttgtcgata ataggaaagc catgtgatta
      661 ttcacagaca agccaaacta tgccaatcat caatttacct tcaccacagg gtttttttag
      721 taccgcatcc ggaacatcag agagccctgt cttgcttctg ggaagagata tcaatcccca
      781 gacggtgcca gatttaaggt caggtatttt gcaatctgtt gagggtgacc gtcaagcgtg
      841 ggcaagaggc gaggctctgg gggaggtcgg tgtacgggaa gattggtatg cttctgcaga
      901 tcaggtcctc gaacaattgc ctgtttgtgg tcagtggtcg gtctttaaat atgaacagac
      961 tgccagcgaa gtccctgcca ctctttgcgc cgaaagcaca cctcacgatg tggcacaact
     1021 tggcacattg attgattggc tggtcaggtt aagccaacaa agtggtgagc ccatcaggag
     1081 agaagttgtt caagaagtgc ttttggtttg ttttaccagt cgtgtggtca gcgtagatat
     1141 tgaagaggag tcatcggagg cattcattgg cgaggagacg ccagaaccac aagatttgag
     1201 ggcgactctt gattttttgt acgattgttt acgagatgga gaattgcaaa atcacctgaa
     1261 cccacatcat ccctacgata atctaagaat attgcttggt agcatacata gattgcatcg
     1321 cttgatttgc ctgaatgcgc cagattgtct tatgcttgga gatatacgca tattaagcgg
     1381 acgcctcatg tcttgtctta agccagaggc cgcattggca ttcactcggg tgaggtacgc
     1441 gcccctggtt caatccattg aacgtcaccc cgcgttagcc ccctttgcgg cacaggcaaa
     1501 aaaaatcgca tgccttattg agatgtcgtg ttatcaaaaa agcgctgtga gatttgaatc
     1561 gatcacgacc atgagtcatt tgtttggtct gtgcctgcct gaacaccaac cagcccttgc
     1621 cgacgcgcta ttagaagtga tcaacaagac tcccgcggag gaggtatcaa ggctgattga
     1681 atttatgggt tgtgtggagg aggatgtcgt cggtgaagct accgttatcg ttaatctgaa
     1741 cacaattctc agaaaggtaa ctgggcgccg ggtactggct attcagaatg tggttttttt
     1801 gcaggaagtc attcggagaa cacatggttg tgtccaaaga cagcaaggcc tgatgaaaga
     1861 tcccgattcg gagttgagta gtggttttga acagttggaa gatcctctat cgctattgga
     1921 tggtttgcga caaatcttgt cttatcaccc aacgccggaa gtgctccggg ccatgatgac
     1981 tgccgacgct cagagcggca cgcaaaatca cctcatctgg gttctcaaaa acatccccaa
     2041 gggcagtcag actcgtgacg cgcgacggtt tttggcggac tatctgttgt tgcggacgcg
     2101 gctcaaatta ttcctggccc gtgcgcaacg tggggattgg acagatgaca acattggcaa
     2161 actgagagag ttgtatgctt gggagcatga tcctgctttg cggggcgaga tcagaacgcc
     2221 ggaggatgcg gccagtgatc gggatttgat tgcggagatg aaggatgaag tggcgttatt
     2281 gggttatgac gactacaagc aatttcgtga ttatgggttc gatgtcactc ttcctctgga
     2341 gactgtggca aaactacagg agatcgattc tttttttgat gacatgctca tcagcatcaa
     2401 tagaactcaa acagattcgg ctaaaagggc agagaccatg ttggccttta ttgagttttg
     2461 cagggtggtc accaatcttg gcaatttgtt gccgggactt gcttttggtg aaatcattga
     2521 gggcatgcgg gcccagtgcg ctcaaaaaag acggcgcgaa ttcatggatg cggcagagat
     2581 caagaagatc accgagacga tcaaattggc ggccctcgat gatttttttg ccaagagttc
     2641 gctacccgca gacacgcaat cgaagattcg tgagcagatg cagaatcccg aaagccctct
     2701 gagtgagcct gagttaatca atgagattgt ggcctattac cattttcagg gccccggagg
     2761 cagagaggcg gttaacgagt atttgcggat gattgccaat caggggcgac acgttgcccg
     2821 caaaggagtc ttaccagttg agagagaggt aactctaatg cgtcgacaga ccagcaggag
     2881 ggaaggtttt ttgactgttt atcgttccat tttggaacac ctcatggtgg agggttcagc
     2941 agaaattgca gaggctgatt tgagaaccgt tattatggac gttgaggtgt gcttaaggga
     3001 cggacggcgt atatcccatg acaaactgtt tgagtggcaa caacggctac aagagtgcat
     3061 gagtcaacag cctaacgggc agatcactga ggttctgcac gatctggaac agatcaggac
     3121 cctgcaggaa aatcgaaatc aagccccagg aaccacctat gattttttta taacggacga
     3181 tcccgtagtc atcgcgcgcg caggggaaga acctctgaga acctgtcaac gtatttcatc
     3241 tcctggtgca tacaatcgtg aaggacagcc gctatggcgt gccatgcacc cgcatgtgag
     3301 tctggccggt cttaaggatc cccatacggg caaggtagtg gcgcgcgtgc ttttggagta
     3361 tgtgcgtccg ggcattatgc tggtggagcg gcattatctt gcgggcggta tttcaatgga
     3421 ggagttcgat tacgcactgt tgtctgaact ggcaaggcaa aggcggcttc gccacgtggt
     3481 ttttgccgat aataggggac tgcctttcac ggctgagaca gtgctggttg gggaaattcc
     3541 ggagtatttt tatcacgata ctttttattc gcatgcagaa ttcagagcga tcagactgga
     3601 tgacaaagat acccgtatgg cattacaaac tcacggtgtc ctctttatga ggccggtgaa
     3661 aaaaagaaaa aagataactg gttagtgggc gtcatgtgtg cgtcatgtgt gcgtgtgtcg
     3721 gtaatctgca aacaggttct gccacttggg gggcaggtgg gcgaggacga gggtgccgtc
     3781 atcggtattt tccgtcgaaa tcaca
//
LOCUS       DATDUN010000721         1296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_238768, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000721 DATDUN010000000
VERSION     DATDUN010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238768"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VJC18_10015"
     CDS             <1..278
                     /locus_tag="VJC18_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45759.1"
                     /translation="LPKGADNNVMVTFKRSDNSVLGALVLSPEQPSIKNFTYRLGDQK
                     IHIQGIQLGLPVYERSMGSVVINCKISDGNGRNTERYKGMIASWPYK"
     gene            complement(288..419)
                     /locus_tag="VJC18_10020"
     CDS             complement(288..419)
                     /locus_tag="VJC18_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45760.1"
                     /translation="MNLYPQIHIFRQGLVWLTAPEDTRGERARKILHSLIAAGMVGE"
     gene            complement(438..767)
                     /locus_tag="VJC18_10025"
     CDS             complement(438..767)
                     /locus_tag="VJC18_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45761.1"
                     /translation="MSGVVHRAPVVGKDNYLAVPNESRQPLVGSHFADLRLPLIHAKT
                     NGYREAIKMPTAEQELAKNERVKVLFNAISVAKKESELFIVGGGGGVQNGKIAGGLQI
                     LTRVEYD"
     gene            1067..>1296
                     /locus_tag="VJC18_10030"
     CDS             1067..>1296
                     /locus_tag="VJC18_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45762.1"
                     /translation="MTKLLLLTTSCVICLVIAGCGGGQDDFEQLQSLSPINELIGTWE
                     IEGIANKATLTMDGEATPDDGNFMTFASNITIT"
BASE COUNT          348 a          341 c          282 g          325 t
ORIGIN      
        1 ccctcccgaa gggcgcagac aacaacgtga tggtcacctt caaaagaagc gacaacagcg
       61 tcttgggggc cctggttttg tcacccgagc aaccctcgat caaaaacttc acctacaggt
      121 tgggggatca gaaaatacac attcaaggca tccagttggg gcttcctgtg tatgagcggt
      181 ccatgggctc tgtggtcatc aactgtaaaa tcagcgatgg caacggccgc aacacagaac
      241 gatacaaagg catgatcgcc tcctggccgt ataaataaag ggttcagtca ttcccccacc
      301 atccccgcgg ctatgagact atgcagaatt tttctggcac gctcacctct tgtatcttct
      361 ggtgccgtta accaaacgag gccctgtcta aagatatgaa tttggggata taaattcata
      421 actttgcctt ctaaccatta atcatattca actcgagtca atatttgtaa cccgcctgcg
      481 atttttccat tttgtacgcc accgccccct cccacaatga atagctcaga ttctttcttg
      541 gccacactta tcgcattaaa cagaacttta actcgttcgt tcttcgctaa ctcttgctcg
      601 gctgtgggca tttttatcgc ttccctgtag ccattcgtct ttgcatgaat caacggcaag
      661 cgtaaatctg caaaatgtga tcccactaat ggctggcgcg attcattggg aacagccaga
      721 taattatcct tgccaacaac cggcgcacga tgcacaacac cgctcacaaa ggaggaacca
      781 agaggctccg atccggttat gactttgggg gctgtacaat accggtgcag atggaatgac
      841 cataatgata acatgatcgc caagactatc ggcacatgcg gacaatagtt gctttttttg
      901 aattcaccgc acaactttcc gtccacaatt gtggacggtt tttttaacgc aacctttgcc
      961 gacgctggtc gataatcatt gcacgtacac gtattgtgta ttttagcagt cagaaaggcc
     1021 caacagcgat cgtcgccttc cgaacgaaag gaaccatatg cacgccatga caaaattatt
     1081 actcctcacc acaagctgtg tgatctgtct cgtcattgcc ggttgcggcg gcggtcagga
     1141 tgattttgaa caactgcaaa gtctttcacc aataaatgaa ctcatcggca cctgggaaat
     1201 tgagggcatc gcaaacaagg ccacgttgac aatggatgga gaggcaacgc cggatgatgg
     1261 caatttcatg acgtttgcca gcaacatcac tatcac
//
LOCUS       DATDUN010000722         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_570072, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000722 DATDUN010000000
VERSION     DATDUN010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570072"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VJC18_10035"
     CDS             <1..450
                     /locus_tag="VJC18_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HLD45763.1"
                     /translation="KCADHDVVLIHDAARPVIDGELIRRVISSTPKMGAVIPVVPVAD
                     TIKEVDSDDVVVKTVNRDRLRSVQTPQGFLYSHLRKAYNTLDFKDARFTDEAMLMEAL
                     GIKVATVEGDPHNIKITTPFDLQIVEELLKQRIEERIGCSQTHVVSV"
     gene            514..720
                     /locus_tag="VJC18_10040"
     CDS             514..720
                     /locus_tag="VJC18_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HLD45764.1"
                     /translation="MDCHKEKNLKNCNCTYTPCSHKGACCDCLSYHLKNRELPACVFP
                     PNAERTYDRSFEHFARLVQGGVVK"
     gene            776..1186
                     /locus_tag="VJC18_10045"
     CDS             776..1186
                     /locus_tag="VJC18_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HLD45765.1"
                     /translation="MKPKDIRWQQRFQNFKKAFAQLEQAVALKTPDIIQQQGIVQCFE
                     YNFELSWKTLQDFLSTERGYSDIKGPRPVLEQAFQDGILRDGVTWLDMLKSRNLTAHL
                     YDEEEVLQILKKIREDYFPLFSELKLFFEKHHDR"
     gene            1176..1475
                     /locus_tag="VJC18_10050"
     CDS             1176..1475
                     /locus_tag="VJC18_10050"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLD45766.1"
                     /translation="MTDKYGIPDNLWSQIIDLFPPYQQIESVVLFGSRALGTFHNGSD
                     IDLCLKGLCESDIKLKILNQYEELYFPWKLDLIIWQNIQNQDLKDHITRVGIIVC"
     gene            complement(1619..>2174)
                     /locus_tag="VJC18_10055"
     CDS             complement(1619..>2174)
                     /locus_tag="VJC18_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45767.1"
                     /translation="DARLAVDPETGDVLARCLTRVSAQENVLRYTQLPVENDDNIPSL
                     MDMVYKALQPGGQPIFAHCRGGRHRAGLMATYSLIQYCGLSNDPHTDEKGNVLQDTFG
                     GILSDASEYWYRNTGGKPDDPRDPTNPDYDPTDSEWIREFTETMNMIGSFIPLPGLIL
                     SPELQQTVCPPAKDPVDPKNEDHF"
BASE COUNT          570 a          491 c          512 g          601 t
ORIGIN      
        1 aaatgtgccg atcacgatgt ggtcctcatt cacgacgcgg ccagacctgt gattgatggt
       61 gagctcatca ggcgcgtgat ttcctcgacg ccaaagatgg gtgccgtgat cccggtggtg
      121 cctgttgccg ataccatcaa ggaagtcgat tcggatgacg tcgtggtcaa gacggtcaac
      181 cgcgaccgac tgcgttctgt gcagacacca caaggttttt tatacagcca tttgagaaag
      241 gcctacaaca cgcttgattt caaggatgcc cggttcacag acgaggccat gctcatggag
      301 gctctgggaa tcaaggtggc gactgttgag ggtgatcccc acaacatcaa gatcactacg
      361 ccgtttgatc tgcagattgt ggaggaatta ctgaagcagc ggatcgagga gcgcatcggt
      421 tgctcgcaga cacatgttgt ttctgtttga gtcctgaaac cttgttgctt tatttcttca
      481 ttgtttgatt gtttcattga ttcatttatt tttatggatt gtcacaaaga aaaaaatctc
      541 aaaaactgca actgcaccta cacgccttgt tcccacaagg gggcttgttg cgattgcctt
      601 tcatatcatc tcaaaaaccg tgagttgccg gcctgtgtct ttcccccaaa cgccgaaagg
      661 acctacgatc gttcctttga acactttgcc agattggtgc agggaggcgt ggtgaaataa
      721 tccagttttt ttgcaatttt tgacaattaa cccattgttc tttatagagt gctccatgaa
      781 gcctaaagac attcgttggc aacaacgttt tcaaaacttt aaaaaagcct ttgctcaatt
      841 agaacaggct gtggccctga aaacaccgga tatcatccaa cagcagggaa ttgtgcagtg
      901 tttcgaatac aattttgaac tttcatggaa aaccctgcaa gatttcttgt cgacagaacg
      961 tggctattcc gacattaaag ggccaagacc tgttttggaa caagcctttc aagatggcat
     1021 ccttcgtgat ggggtgactt ggcttgacat gctcaagagt cgtaatttga ctgcccacct
     1081 gtatgatgaa gaagaagttc tgcagattct caagaagatt cgggaggatt attttcctct
     1141 tttttcagaa ttaaaattat tttttgaaaa gcatcatgac agataaatat ggcattccag
     1201 ataatctctg gtctcaaatc atcgatttat ttcctcctta ccaacaaatc gaatctgtcg
     1261 ttttgtttgg gtcaagggcg ctgggaacct tccataacgg ttctgatatc gatctctgtc
     1321 tgaaaggatt atgtgagtct gatatcaaac tcaaaattct taaccaatat gaagaattgt
     1381 attttccctg gaaactggat ttgattatct ggcaaaacat tcagaatcag gatcttaaag
     1441 atcatattac aagagtcggc atcattgttt gttaggggta ttgaagggct agcgtgcgcg
     1501 cgatccacag atcaaggaaa tacccgcacg tcaggtcctc ccgaaaaacc aagatactcc
     1561 atggtaaacg tggatattaa ccaggcaata agatatagcc cacacacaat ccaattgatc
     1621 aaaaatgatc ttcatttttc ggatcgactg ggtcttttgc cggcgggcac acggtttgtt
     1681 gcagttcggg tgaaaggatc aggccgggca gggggatgaa actgccaatc atgttcatgg
     1741 tttcggtaaa ttccctgatc cattcactat ctgtgggatc gtaatccggg ttggtgggat
     1801 ctctcgggtc atcaggtttg ccacccgtgt tgcggtacca atattccgag gcatcgctca
     1861 agatgccacc aaaggtgtct tgcaacacat tgcctttttc gtcggtgtga ggatcgttgg
     1921 aaagaccaca atattgaatg agagagtaag tcgccatcaa acctgcacgg tgtctgcctc
     1981 cgcgacaatg ggcaaaaatg ggctggccac ccggttgcag ggccttataa accatgtcca
     2041 tgagcgatgg gatgttatca tcattttcca ccgggagctg agtgtaacgc aacacgtttt
     2101 cctgggcact gaccctcgtc aaacagcgcg ccaaaacatc acctgtttct gggtcaacgg
     2161 ccagtctggc atca
//
LOCUS       DATDUN010000723         3565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_529842, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000723 DATDUN010000000
VERSION     DATDUN010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529842"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VJC18_10060"
     CDS             <1..318
                     /locus_tag="VJC18_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45768.1"
                     /translation="SDMYSAHAGYEGLNNTTNLIIFQDFINITRRTLTLFIIRMALGE
                     AVAIFGFVLTVLTHSFYTMLPFALVAFVLQFLFGPLYANARKASGDTYNMNENNTTDS
                     AGQ"
     gene            complement(296..589)
                     /locus_tag="VJC18_10065"
     CDS             complement(296..589)
                     /locus_tag="VJC18_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45769.1"
                     /translation="MKTKSTCALLLVFAQLLFVSCAARNNLASEFVYHQNQQVRVGLN
                     SSRATQAQEHYQPEIDGADFDSFDDKAGAVLIGLLGVGLTVSAIVIPIVLLNR"
     gene            complement(593..1126)
                     /locus_tag="VJC18_10070"
     CDS             complement(593..1126)
                     /locus_tag="VJC18_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45770.1"
                     /translation="MKRSIVGFMVIVLCVVASCSVFTNKDKDEVKINLPGVSIETKGD
                     DAKVKLPGLDIKTEADKTEVNLPGMTIKTDESSATGEDKDTEKEAPEQPSTLKVETDK
                     DGQAQTMTINSDGKTIQIKVDEKNGIVQIPGMNIQVSKDGSVVTTQPTDNVPQQPVVE
                     IKNAEEAGSADEEAVEE"
     gene            complement(1206..1841)
                     /locus_tag="VJC18_10075"
     CDS             complement(1206..1841)
                     /locus_tag="VJC18_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HLD45771.1"
                     /translation="MKDCLKPTYYIDFEDKAVMDFVHKHTRQGATLPQQAQSLFYAVR
                     DGIQYNPYEIETSQASLKASATLGRGHGFCIAKAVVMVAACRAVGIESRLGFADLINH
                     RLPEKLKAMLGSNHIVYHGYCELYLHNKWIKVTPVFPLALCQEHGYLPVSFDGISDAI
                     FQKQDVRGNPHMEYIGFHGVFDDLPFDEILKAFQTCYPKYFTKRDNAVGVS"
     gene            1948..2619
                     /locus_tag="VJC18_10080"
     CDS             1948..2619
                     /locus_tag="VJC18_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLD45772.1"
                     /translation="MNIINFLKTIFLFEHLSPNEMALVAKNATEIKLKKNGFLFCDGD
                     PADAFFVVALGTIKVFKASASGAEQPLHTQPARSLIAEAAIFGLDYYPANAKALEDSL
                     VVRIPKKEFVDMIHERPALSLKLMAGYSKRLREFVRTIENLSLNNPQQRLSSHILGNL
                     RTEGKKHYYPLSTSKKELANILGMTPETLSRCLKIMKNKKLLSEKNGELIVASKEKFA
                     RCCDD"
     gene            2697..3467
                     /locus_tag="VJC18_10085"
     CDS             2697..3467
                     /locus_tag="VJC18_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLD45773.1"
                     /translation="MKRNIIKINEDLCNGCGLCVTGCSEGALQIVNGKAKLVREDFCD
                     GFGDCMGDCPTGALIIEERDAPDFDEPAVKQHLLTTQGPQAVNKMVLAQNRHAQKHTP
                     PSGCPGMRMMHKQQESEDKPVSANVLPQVMRSELRQWPVQLHLVNPLAPYFQNAELVV
                     LSTCSPVACPDVHWRFIRGRAVVVACPKLDNKSGYVEKLAAIIRTAHTPKIITVMMAV
                     PCCGGLGQIVAEAIKLSGRSDVCFENKTITLEGIIITS"
BASE COUNT          946 a          936 c          776 g          907 t
ORIGIN      
        1 tcggatatgt attcagcaca cgcgggttat gagggcttga acaacacaac caacctcatc
       61 atttttcagg atttcatcaa catcacccga cgaacgctga ccctattcat catccgaatg
      121 gccttgggag aagctgtggc tatttttgga tttgttctga ccgtcctcac gcattctttt
      181 tacacaatgc taccctttgc cttggtggca tttgttttac agtttctatt tggccccctg
      241 tatgcaaacg caagaaaagc cagcggagac acatacaaca tgaatgagaa caacactacc
      301 gattcagcag gacaataggg atcacaatcg cgctcaccgt gaggcccaca cctaacagac
      361 caatgagtac ggcccccgcc ttgtcgtcaa acgaatcaaa atcagctccg tcgatttcgg
      421 gctgatagtg ttcttgcgcc tgagtggccc tgcttgaatt caaaccaaca cgcacctgct
      481 ggttttgatg atatacaaac tcgcttgcca aattgtttct ggccgcacaa gaaacaaaca
      541 gtaactgggc aaaaaccaac aacaacgcac atgtcgattt tgttttcatg aattattctt
      601 cgacagcttc ttcatcagcc gatccggcct cttccgcgtt ctttatttcg acaacgggct
      661 gttgcgggac attgtccgtg ggttgtgtgg tcaccacaga cccgtccttg ctgacctgaa
      721 tgttcatccc cgggatttga acaatgccat ttttctcatc gaccttgatc tggattgtct
      781 tcccatcgga attaatggtc atcgtctgcg cctgaccatc tttgtctgtt tccaccttaa
      841 gcgtcgaggg ctgctccgga gcctctttct ctgtgtcttt gtcctcaccg gttgcagagg
      901 actcatctgt cttgatcgtc atgcccggca aattcacttc ggtcttatct gcttcggtct
      961 tgatgtcaag ccccggcaac ttgaccttgg catcgtcgcc cttagtctcg atgctcacac
     1021 ctggcagatt gattttaacc tcgtctttat ccttgtttgt aaaaaccgag cacgacgcaa
     1081 caacacagag aacgatgacc ataaaaccaa caatactgcg cttcataaaa agcctcctta
     1141 aaactttaaa cggtgattag tctttcaaaa gaacttcatt gccgtagctc tttaccagca
     1201 aaacgtcaag acaccccaac agcattatct ctctttgtaa aatatttggg atagcaggtc
     1261 tgaaaagcct ttaggatttc atcgaagggc aaatcgtcga acacgccatg aaaaccgata
     1321 tactccatgt gagggttccc tcgaacatct tgtttttgaa atatggcatc tgagatgccg
     1381 tcgaacgata cgggaaggta accatgctct tgacataacg ccaatggaaa cacaggtgtg
     1441 accttgatcc acttgttgtg taaatacaac tcacaataac cgtgatacac aatgtgattg
     1501 cttcccagca tggccttgag tttttctggt agtctgtgat tgatcagatc ggcaaacccc
     1561 aaacgactct cgatcccaac agcacgacaa gcggccacca tcaccacagc cttggcaatg
     1621 caaaatccgt gtccccggcc aagagtggca cttgccttga gtgatgcctg gctcgtttcg
     1681 atttcgtaag gattgtattg aatcccatcc ctcacggcat aaaaaaggct ttgggcctgt
     1741 tgtggcaacg tcgccccctg ccgggtatgt ttgtggacaa aatccatgac ggccttatct
     1801 tcaaaatcaa tgtaatatgt gggtttgaga caatccttca taacaacctc ctatcaatgc
     1861 gagaccccgc ctggcttttc tattgatcat ggcaatgttt gcccctatca tcccccatca
     1921 tccatacgaa cgcaagggtt cccgcacatg aacatcatca attttttgaa aaccattttt
     1981 ctgttcgaac acctgtcgcc aaacgaaatg gccctcgtgg ctaaaaacgc gaccgaaatc
     2041 aaactcaaaa agaatggttt tttgttctgc gacggggatc cggctgacgc ttttttcgtt
     2101 gttgccttgg gaacaatcaa agtttttaag gcctctgctt cgggcgcgga gcaaccgcta
     2161 cacacacaac cagcaagatc gctcatcgcc gaagccgcca tttttggtct ggactattat
     2221 cccgcaaacg ccaaggccct tgaagactcc ttggtggtca ggattcccaa aaaggaattt
     2281 gttgacatga ttcacgagcg ccccgctctt tcgttaaaac tcatggcggg ttattccaaa
     2341 agactgcgcg aatttgttcg cactatcgaa aacctctcac tcaacaaccc acaacaacgc
     2401 ctatcctccc acattctggg caacctcaga acagagggaa aaaaacatta ttacccgttg
     2461 agcacttcaa aaaaagaact ggccaatatt ctaggcatga cgcccgaaac cctttcgcga
     2521 tgtcttaaaa tcatgaagaa caaaaagctt ttgtctgaaa aaaacgggga actcattgtg
     2581 gcctcaaaag agaaatttgc acgctgttgc gacgattgat atttcctccg ttttcttgat
     2641 tccaatcaag gcgtcttttg tctcgttatg agaatatcat gaggggaaaa agtcgcatga
     2701 aacgcaacat catcaaaatc aacgaggacc tttgcaacgg ttgtggcctg tgtgtcacag
     2761 gctgttccga aggggccttg cagattgtga atggcaaggc caagctcgtc cgtgaggatt
     2821 tttgtgatgg ctttggcgac tgcatgggag actgtcccac gggggccctg atcatagaag
     2881 aacgcgacgc accggatttt gatgagcctg ctgtcaaaca acatctgctc accacccaag
     2941 ggccacaggc cgtcaataag atggtgctgg cacaaaacag gcatgcgcaa aaacacaccc
     3001 caccttcggg ttgtcccggc atgcgtatga tgcacaagca acaagaaagt gaagacaagc
     3061 ctgtttcagc caacgtcctg ccacaagtca tgaggtcaga gcttcggcaa tggcccgtgc
     3121 aattgcatct ggtgaatccc ctggccccgt attttcaaaa cgccgaactg gtcgtcctga
     3181 gcacctgctc ccctgtggcc tgtcccgatg tgcactggcg attcattcgc ggcagggcag
     3241 ttgtggtggc ctgtcccaaa ctcgataaca agagtggcta cgttgaaaaa ctggccgcga
     3301 tcatccgaac ggcccacact cccaaaatca tcacggtgat gatggcggtc ccctgctgtg
     3361 ggggtctggg tcaaattgtg gcagaggcca tcaagctatc cggaagaagt gatgtgtgtt
     3421 ttgaaaataa aactatcact ttggaaggaa taatcataac atcataaact ttaatataaa
     3481 ggagaaaaca tgttctgtta tcaatgcgaa caaacctcac acggaaaagc ctgcaccaca
     3541 aaaggtgtgt gtggcaaaga ttctg
//
LOCUS       DATDUN010000724         4789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_615273, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000724 DATDUN010000000
VERSION     DATDUN010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615273"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..907
                     /locus_tag="VJC18_10090"
     CDS             59..907
                     /locus_tag="VJC18_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD45774.1"
                     /translation="MNSLYVTAMCDAQTEIMSYECYRLFLRDYYENKKKQRGGYSYRK
                     FSRDFGFSSANHLYLVIEGKRNLSDDAIVRIKSLIKGTAQEKRYFETLVRYNQATTDV
                     EKQKNKAKLDDIKAMCRAVVDDKKYSYLSGWHIPVIREMIALKGFVPSLDWVEKNIYP
                     PVKRAKIREAFQILQKLGMLEKKAGRWIQKDPHLTTPPLVSSDIVFAYQQETLDLSQK
                     AIHIGGGKRNISTMTLGLTQKQYEWLDKRINDFRDEIQQELQDTPETNTTVAMLNIQL
                     FPVIRR"
     gene            complement(1013..2359)
                     /locus_tag="VJC18_10095"
     CDS             complement(1013..2359)
                     /locus_tag="VJC18_10095"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HLD45775.1"
                     /translation="MIIYCILFAILVNCGHEAGPLTSSESLEPEDDPIDETTTEVASE
                     EKAEDCSQVQTCYLDSDQDGFGDSLESITLPTCPAEGIPVCGDELGYVADATDCDDAN
                     LDVNPDTSELCNDAIDNDCDGRTDEDCSTAQPSNTERAPLYMTTMTHMEGLFKDDRVE
                     SLFLNHVEALRYGMDLAEEYGAILTIESEKPFAIANTKWGIDIMAEIRDRGFGVGTHC
                     DIGFNSPLMSVDEFAALFAANKTLVDDLVGAENNLGCSGGGSVNDWAQAASLAGFKYL
                     DGIVSMHYLSMPLENRPGPEWTDDYIRNGHYHLNAPVDINERIYLFGVADATDFVADE
                     EPVITISSGEMGLLSAVAEGGRESGNDIIMNDDGFMFDQNDTDVLVDIIRAVDETRDP
                     AQIAKMCVYLPVTFFSNANGEALRYFFATMQELQNEGIITWATQRQVYEAYVDATE"
     gene            complement(2572..3171)
                     /locus_tag="VJC18_10100"
     CDS             complement(2572..3171)
                     /locus_tag="VJC18_10100"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HLD45776.1"
                     /translation="MSLYQFDQKYCNQGSLVAGFDEAGRGAWAGPVSAACVIMPQSPL
                     ITGINDSKKLTPKKREKLFEEITATCVSYGVALVDSTEIDRINILEATKQAMVLALSQ
                     LKVMPAILLIDGDMTIDSKIPQEAIIDGDAKSYCIAAASIIAKVTRDRLMVDLSRTFP
                     QYGFEQHKGYGTKVHQDALKQFGCLPIHRKSYAPIRSML"
     gene            complement(3244..3657)
                     /gene="rplS"
                     /locus_tag="VJC18_10105"
     CDS             complement(3244..3657)
                     /gene="rplS"
                     /locus_tag="VJC18_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLD45777.1"
                     /translation="MNAIDWINQKYHKEATFPEFKSGDSVKVHVKIKEGDKERVQVYE
                     GVVIAIHRSGAGSTFTVRKMSYGVGVERIFPFSNPAIQKIEVSSVGKVRRAKLYYLRD
                     LSGKKARIPSQDRADLLLAQKDADQATPAESADQQ"
     gene            complement(3676..4236)
                     /locus_tag="VJC18_10110"
     CDS             complement(3676..4236)
                     /locus_tag="VJC18_10110"
                     /inference="COORDINATES: protein motif:HMM:NF021455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLD45778.1"
                     /translation="MPDLYVALIHHPIVNKEGKTVTTSVTNFDLHDLSRNCQTFGVKK
                     LFIVTPMINQLNMVNYIRDYWQEGFGSQYNPSRKQAFDVLQVSPNIEDTCLTIKKLSG
                     KEAHLVATTAKYSDKSVGYSFVRNQIEKGDRPILLVFGTGFGLAPEFFAKVHYVLEPI
                     YGAGDYNHLPVRSAVAIILDRLVGRN"
     gene            complement(4303..>4789)
                     /gene="trmD"
                     /locus_tag="VJC18_10115"
     CDS             complement(4303..>4789)
                     /gene="trmD"
                     /locus_tag="VJC18_10115"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HLD45779.1"
                     /translation="AVRDIKQMYSAEHVVLTSPAGHLLTPDKAKALSQKKSILFLCGR
                     YEGVDQRAIDLVVDEELSIGDYVLSGGELASGVMIDVISRYLPGVVGKDQSVRLESHE
                     NILLEHPHYTRPEEFEGLMVPEVLISGHHKNIAAWQEEQAVLRTKERRNDLYQKFIRE
                     S"
BASE COUNT         1305 a         1209 c         1047 g         1228 t
ORIGIN      
        1 cctgcaaaaa gcgtggtttt tcaaggtttt gtttactcag taaacactgg aaaacagcat
       61 gaattcgctg tatgtcactg ccatgtgcga tgcccaaacg gaaatcatgt cttacgaatg
      121 ctaccgattg tttttgagag attattacga aaacaaaaaa aaacaacgcg ggggttattc
      181 ttatcgcaaa ttttccagag actttggatt cagttccgcc aatcatctgt atctggtcat
      241 cgagggaaaa cgcaatttat cggatgacgc cattgtcaga atcaaatcgc tgattaaagg
      301 aaccgctcag gagaaaagat atttcgagac actggtcaga tacaatcagg ccacgacaga
      361 cgtggaaaag caaaaaaaca aagccaaact ggatgacatc aaggccatgt gtcgcgcggt
      421 ggtagacgac aaaaaatact cctacctctc cggttggcac atccccgtga tccgcgaaat
      481 gatcgcattg aaaggatttg tccccagcct ggattgggtg gaaaaaaaca tatacccccc
      541 tgtcaaacga gcgaaaatca gggaggcctt tcagatcctg cagaaactgg gcatgctgga
      601 gaaaaaggct ggacgatgga ttcaaaaaga ccctcatctc acgacgccac ctctggtgtc
      661 ttctgatatt gtctttgcgt atcagcagga aaccctggat ttgtcgcaga aggccattca
      721 tataggcgga ggcaaaagga atatttccac gatgacactg gggttgacgc aaaaacagta
      781 cgaatggctc gacaaacgga tcaacgattt tcgcgatgag attcagcagg aattgcagga
      841 cacgcctgag accaacacga ctgtcgccat gttaaacatt cagctctttc ccgtcatcag
      901 gcgttgaggt cagatcaaag accgtcccgc ctgcatctac tgtggcgcac aaataaaggt
      961 aaagatgttt tgaaaatgat ttttaattcc tcactccgtc attgcctgtc cttcattcgg
     1021 tagcgtcaac ataggcctca tacacctgcc tttgtgtggc ccaggtgatg atgccctcgt
     1081 tctgtagttc ctgcattgtg gcaaagaaat agcgcaacgc ctccccgttg gcgttggaaa
     1141 agaatgtgac gggaagatac acacacatct tggcaatctg cgcaggatcg cgcgtctcat
     1201 ccactgcccg tatgatatcg accaacacgt cagtgtcatt ctggtcgaac atgaatccgt
     1261 catcgttcat aatgatgtca ttgcctgact cacgcccacc ctcggcaaca gctgataaca
     1321 aacccatctc gccagatgaa atggtgatca ctggttcctc atcggcaaca aaatccgtcg
     1381 cgtcagcgac accaaacaag taaatacgtt cattgatatc aaccggtgcg tttaaatggt
     1441 aatgtccgtt acgaatataa tcatcggtcc attcaggacc gggtcgattt tcgagcggca
     1501 tggagagata gtgcatgctg acgatgccat ccagatactt gaagcctgcc agactggcgg
     1561 cttgagccca atcatttact gaaccaccac cggaacaacc caaattgttt tccgcgccaa
     1621 ccagatcgtc aacaagcgtt ttgtttgctg caaacagggc cgcaaattcg tccacagaca
     1681 tcaagggtga attgaaacca atatcacaat gggtccccac accaaaaccc cgatcacgaa
     1741 tttcggccat aatatcaatt ccccatttcg tgttggcaat tgcgaaaggt ttttcggact
     1801 ctatggtcag gatggcgccg tattcttcgg ccaagtccat accataacgc agtgcctcta
     1861 cgtgattgag aaagagactc tccactcggt catccttaaa gagaccttcc atgtgggtca
     1921 tcgtcgtcat atacaagggt gctctctctg tgtttgaggg ctgtgctgtt gagcaatcct
     1981 catctgtccg accatcacag tcgttatcaa tagcatcgtt gcaaagctct gaggtatcgg
     2041 gattaacatc aaggttagcg tcatcgcagt ccgtcgcatc agcgacatac ccgagctcat
     2101 caccacagac agggatcccc tctgcagggc aggtaggcag tgtgatggat tcaagggaat
     2161 ctccaaaacc atcctgatcg ctgtctaaat aacaggtctg gacctgcgag cagtcttcgg
     2221 ctttttcctc actggccact tcggtggttg tttcatcgat aggatcatct tccggttcca
     2281 aactttctga actggtcagg ggacctgcct catgaccgca attgaccaag attgcgaaga
     2341 gaatacaata aatgatcacg gaccatgtca atccgatcga ttggaaacga ttgcttatga
     2401 aattttttgc ctgcatattc acatcctctg tgaatacctt aagcaatagt catgccacag
     2461 aatgtttcgg caggatgcct caacgcacga catccttaac aaaaataatt tcaaaatgag
     2521 atgcttatga ttttttggac aataatttga tcacgttgag aaatgtgctc gtcaaagcat
     2581 ggaccgaatt ggcgcgtacg atttgcgatg gatgggcaga cagccaaact gcttgagcgc
     2641 gtcctgatgc accttggtgc cgtagccctt gtgctgttcg aacccgtatt ggggaaaggt
     2701 gcgcgacaaa tcaaccatca aacgatcacg cgtgaccttg gcaatgatcg acgccgcggc
     2761 aatgcaatag ctcttggcat cgccatcgat gatggcctcc tgcgggattt ttgaatcaat
     2821 cgtcatgtcg ccatcaatga gcaaaatagc cggcatcacc ttcaattggg acaaggccag
     2881 caccatcgct tgttttgtgg cttccagaat attgatgcgg tcgatctctg tcgaatcaac
     2941 caaggccacg ccataactca cacacgttgc cgtgatttct tcaaaaagtt tttcgcgttt
     3001 tttgggagtc agtttttttg aatcgttgat gccggttatg aggggacttt gtggcatgat
     3061 cacgcaagcc gctgagacag gaccagccca cgcgccccga cccgcctcgt caaaaccagc
     3121 caccaaagaa ccctggttgc agtatttttg gtcaaattga taaagtgaca tgccccacaa
     3181 atgcataagg gccggttggc accgacccct aggcatgtaa aaaacaaatt gataaagaca
     3241 agcttactgc tgatcagcgg attcggccgg ggtcgcctga tcggcatctt tttgtgcaag
     3301 caataaatca gcacgatcct gagagggaat gcgtgccttt ttacccgaca aatctcttag
     3361 gtaatacagt ttcgcgcgtc tcactttgcc gacactggac acttcgattt tttgaatggc
     3421 agggtttgaa aatggaaaaa ttctttcaac ccccacaccg taagacatct tgcgaaccgt
     3481 aaatgttgaa ccggcaccac tgcgatgaat ggcaatcaca accccttcat aaacctgcac
     3541 gcgctcttta tcgccttcct tgatcttgac atgcaccttg acgctgtcgc ccgatttaaa
     3601 ttctggaaat gttgcttcct tatgatattt ttgattgatc caatcgattg cattcataat
     3661 aacctcttct gacattcaat tccgaccaac tagcctatct agaatgatcg cgacagcact
     3721 cctcacgggc aaatgattgt agtcaccagc accgtaaatc ggctccaaca catagtgaac
     3781 ttttgcaaaa aactcagggg ccaacccaaa tccagtacca aaaacaagca atatgggccg
     3841 atcacccttc tcgatctggt tcctcacaaa tgagtaaccc acagatttat cactgtattt
     3901 tgccgtagtg gcaacaaggt gggcttcttt accactcagt tttttaattg tcaaacaggt
     3961 atcttcaata tttggtgaaa cctgcagaac atcaaaggct tgttttcgtg agggattata
     4021 ctgacttcca aaaccttctt gccagtaatc gcgaatgtag ttcaccatat tgagctgatt
     4081 gatcatgggg gtcacaataa acaatttttt taccccaaag gtctgacagt ttctcgataa
     4141 atcgtgcaag tcaaaattgg tcaccgaagt ggtgactgtt ttgccttcct tgttgacgat
     4201 agggtgatga atgagggcga cgtagagatc gggcatttat cgtggatcgt agatcgttaa
     4261 tcgttaaccg tggataattt ttttcgatcc acgacccacg attcacgatt cacgaatgaa
     4321 tttttgatac aaatcgttcc ttctttcctt tgtgcgcaaa acggcctgtt cctcctgcca
     4381 tgcggcgatg tttttgtgat gaccgctgat gagcacttct gggaccataa gtccctcaaa
     4441 ttcttccggc cttgtgtaat gaggatgttc caacaaaata ttttcgtgtg actccaaccg
     4501 caccgactgg tccttgccca ccacaccggg aagataacgc gaaatcacgt ctatcatcac
     4561 acccgatgcc agttcccctc ccgaaagcac atagtcacca atcgataact cttcgtccac
     4621 aaccaaatca atcgccctct gatccacacc ctcataacga ccacataaaa ataaaatcga
     4681 ttttttttgg ctcagcgctt tggctttgtc gggtgtcaaa agatgacccg ctggtgatgt
     4741 gagaacaaca tgttccgcac tatacatctg tttgatatcg cgcacagcc
//
LOCUS       DATDUN010000725         1116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_201211, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000725 DATDUN010000000
VERSION     DATDUN010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201211"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VJC18_10120"
     CDS             complement(<1..183)
                     /locus_tag="VJC18_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HLD45780.1"
                     /translation="MKSVDVLAFAKENACKMVDMKFVDFLGTWQHFTVPISELGDDVF
                     DEGLGFDGSSIRGWKTI"
     gene            complement(387..>1116)
                     /locus_tag="VJC18_10125"
     CDS             complement(387..>1116)
                     /locus_tag="VJC18_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HLD45781.1"
                     /translation="STGALDVWFMDGTTISSTLTFGVPASGLTLKGTGDFNADGYVDL
                     LLLNETTSFMTIMRKFSSTIVSTTFTPYLGTDFTIVGLGDFNADGKADIVSRRTVFTP
                     TAATRHGEIQLWFMNGASLSAKGYFGTLALPWSLAGIGDFDKDGTDDLFFRNTITQEQ
                     RMWEINSSTSRLLHIIAPVAYPNAADIITEKLADFDGDGDVDILMRDPLTFVVYNWQY
                     ENYSRTSVGYNGTTPMLTLFDMVD"
BASE COUNT          328 a          273 c          253 g          262 t
ORIGIN      
        1 aatggtcttc caaccacgga tcgaggaacc gtcaaaaccg agcccctcat cgaaaacatc
       61 gtcacccaat tcagaaatgg ggaccgtaaa gtgctgccaa gtccctaaaa aatcaacaaa
      121 cttcatatcg accattttgc aggcattttc tttagcgaag gccaaaacat ctactgattt
      181 catgatgatt ctccttgaat attaattaga taggctacaa taattaaatt aataattcac
      241 atcaaaaaca ggggacagtt gggaccggcc cttccctgat aaacaacgcg gagccaatac
      301 gactttatga ggagacagta aagggacaaa tgcaataggc aatgaagtaa tgtatcagtg
      361 aaacaatgaa acaatgaaaa attgcctcaa tccaccatat caaacagggt caacatggga
      421 gttgtgccat tatagcccac tgaggtacgc gaataatttt cgtactgcca gttgtagacc
      481 acaaacgtca atggatctct catgaggatg tccacatcac catcaccatc gaagtcggcc
      541 agtttttctg tgatgatgtc cgccgcgtta ggatacgcaa ccggcgcaat gatgtgcagg
      601 agtcttgagg tggaactgtt gatctcccac attctctgtt cctgagtgat tgtgtttctg
      661 aagaacaggt catccgtgcc atctttatcg aaatcgccaa tgccagctaa gctccatggt
      721 aacgccaacg tgccaaagta ccccttggcg ctgagtgacg cgccattcat gaaccaaagc
      781 tgaatttcac catgacgagt ggccgctgtt ggcgtgaaga ctgtgcgacg tgacacaata
      841 tcggccttgc catcggcatt gaaatcaccc aacccgacaa tggtgaagtc agtgcccaag
      901 tatggtgtga acgtggttga cacaatcgtg ctggagaatt tgcgcatgat ggtcatgaag
      961 gaggttgtct cgttaagtaa caacagatcc acatagccgt cggcattgaa atctccggtc
     1021 cccttaagcg tcaacccgct ggcgggaacc ccaaaggtca aggtggaact gattgttgtt
     1081 ccatccatga accacacatc cagagcgcct gtgctg
//
LOCUS       DATDUN010000726         1425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_370368, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000726 DATDUN010000000
VERSION     DATDUN010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370368"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VJC18_10130"
     CDS             complement(<1..1216)
                     /locus_tag="VJC18_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HLD45782.1"
                     /translation="MIKFGKGNLRVPKWTRPVYMVAGGMTDFRKHYPEKKLEELTMMA
                     FRMMLEENNLKLDPLEVKGLINFACYGEFADHFQDQLLCEAKVHDYLGLDPLFNVGIK
                     TGGATGGSNILMAGSIVASGMADCALALGWERMDEVATWTGNYYISTAACKEFETRLA
                     RNYASYYAPMARRFQEIYGVSPEVRAEIAVKNRGYACHSPFAQQPGKFTIKDVLESPV
                     VSDPLRFLECCAMSVGSAAVLLCSEEMAYKLTDKPVKLYTAGGSHTLRTGDRRHMEIP
                     LLPNEKASDYNKLYADLKEQKSRWPGFESFAATRFASYLCYNMAGIKNPIEDLDLIET
                     HDAFTISDLQTYGDVGLTLYGEEQNYVTSGECYFKNPKTGKPGKCPSNLSGGLLGSMH
                     AVGCTGIFSAAEV"
BASE COUNT          369 a          379 c          329 g          348 t
ORIGIN      
        1 aaacctcggc ggcagaaaag atacccgtac agcccacggc gtgcatgctc cccaacaacc
       61 cgccagacag gttggacgga cacttgcctg gtttgccggt cttgggattt ttgaaataac
      121 actcgcctga tgtcacataa ttctgctcct ccccgtacaa tgtaagaccc acatcaccgt
      181 aagtctgtaa atcggagatg gtgaaggcat cgtgtgtttc gatgaggtcc agatcttcaa
      241 tgggattttt gatgcctgcc atgttgtaac aaagatacga cgcaaaacgc gtggccgcaa
      301 aactctcaaa acccggccaa cgacttttct gttccttcaa atcagcatag agtttgttat
      361 aatccgacgc cttctcattg ggcaacagcg ggatttccat atggcgacga tcgcccgttc
      421 tcaaggtatg cgacccaccg gccgtataca atttgaccgg cttgtcagtc agtttgtaag
      481 ccatctcttc ggaacaaagc aacaccgccg cagacccgac agacatcgca caacattcca
      541 aaaaacgcag gggatcagaa acaacagggc tttccaaaac atcttttatg gtaaatttac
      601 ccggctgttg tgcaaagggg gaatgacagg cataaccgcg attcttgacg gcgatttcgg
      661 ccctcacctc ggggctcaca ccgtaaattt cctgaaaacg tctggccatg ggggcgtagt
      721 aactggcata attgcgggcc aagcgggtct caaattcttt acacgccgct gtagaaatgt
      781 aataattacc agtccacgta gcgacttcgt ccatccgctc ccaacccagc gccaaggcac
      841 aatccgccat gccactcgcc acaatgctac ccgccatgag aatattggag ccacctgtcg
      901 cgccaccggt tttaatgccc acattgaaca gagggtcaag gcccagataa tcgtgaacct
      961 tggcctcgca aagcagttga tcctgaaagt gatcggcaaa ttccccgtaa cacgcaaaat
     1021 tgatgagccc tttgacctcg agcggatcaa gcttgaggtt attttcctcc agcatcatac
     1081 gaaaggccat catcgtcagt tcttcgagct ttttctctgg atagtgtttg cgaaaatccg
     1141 tcattccccc cgccaccata taaacaggac gggtccattt gggaacacgc aaattgcctt
     1201 tgccaaattt tatcataatt taactccttg tcattggagc ccctcgtaac aagggtgtct
     1261 gtaactggtc aagttgaatg tgaaagggtg tacgacataa aagtaggtaa ttttgatttt
     1321 gccccccccg cctgttgtaa ggcgggggtg cccgtagggc gggggtggta gaaaacatga
     1381 ccaatttgaa agattttgag tgattgactt cctctttttt ttggt
//
LOCUS       DATDUN010000727         1350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_370421, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000727 DATDUN010000000
VERSION     DATDUN010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370421"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VJC18_10135"
     CDS             complement(<1..376)
                     /locus_tag="VJC18_10135"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HLD45783.1"
                     /translation="MTKPKALILTGYGINCEEETSYAFGLAGAEAVILHMNDLIAEKN
                     RILDFQILALPGGFSYGDDTGSGNAIANRIKNNLVEFIQRFIEKDTLTIGICNGCQVL
                     ANMGVIPGFEFHESKREVIFTHN"
     gene            complement(380..>1350)
                     /locus_tag="VJC18_10140"
     CDS             complement(380..>1350)
                     /locus_tag="VJC18_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HLD45784.1"
                     /translation="CAPATITRPVLSSKKGVVLSQGINPRYSDVDAYRMAACAIDTAI
                     RHVVAAGGSLEHLALLDNFCWCDARNPVRLRQLKDAARACYDYATAFGTPFISGKDSM
                     FNDFRGYDENGQPVTLSIPPTLLISSIGVMEDVSLAVSLDVKMPGDLVYVIGTTGNEM
                     AGSEYLAYVNEESQSQFVGKNVPEVNAVRARLDYQSLTTAIQKRLVASALGVGLGGLG
                     VALAKAAIAGQLGMDIDLSRVKIAEGVVRNDFLLFSESQSRFVVTVDSRKCQAFEKIM
                     TDVPHACVGSVLREPDFEMKGLNGQRIVNTDVAQLTSAYRKTFAGW"
BASE COUNT          338 a          404 c          311 g          297 t
ORIGIN      
        1 tgttgtgcgt aaaaatcacc tcacgtttgc tctcgtgaaa ctcgaacccc ggaatcaccc
       61 ccatattggc cagcacctga cacccattgc aaatgccaat ggtcagcgtg tccttttcga
      121 taaagcgttg aatgaattca acgagattat ttttgatacg gttggcaatg gcattgcccg
      181 aaccggtgtc atcaccataa gaaaaccctc cgggaagggc cagaatctga aaatccagta
      241 tcctgttttt ttcggcaatc aggtcgttca tgtgcagaat caccgcctca gcgccggcca
      301 gcccaaaagc ataagaggtc tcctcttcac agttgatccc atagccggtt aaaatgagtg
      361 ccttcggttt tgtcatacgt caccacccag caaaggtttt gcggtacgcg ctcgtcaatt
      421 gagccacatc ggtgttcaca atgcgttgtc cgttcagacc tttcatctca aaatcgggct
      481 ccctgagcac actgcccaca caggcgtggg gaacatctgt catgattttt tcgaacgcct
      541 gacatttgcg tgaatcaacg gtcaccacga atcgcgactg tgactcggaa aatagcagaa
      601 aatcattgcg gacaacacct tcggcaattt tcaccctcga caaatcaata tccatcccca
      661 attgccccgc aatggccgcc ttggccagtg ccacacccaa acctcccagc cccacaccca
      721 aagccgaggc caccagacgc ttttgaatgg ctgtggtgag tgactgataa tcgagccgcg
      781 ccctgacagc attgacctcg ggaacatttt tacccacaaa ctgactttgt gattcttcgt
      841 tgacgtacgc cagatattcg gacccagcca tttcgttccc cgttgtcccg atcacgtaca
      901 ccagatcccc cggcatcttc acatcgagac tcactgccaa cgacacatct tccatgacac
      961 caatcgacga gatcagcagg gtcggcggaa tcgacagagt cacgggttgt ccgttctcat
     1021 catacccgcg aaaatcgttg aacatgctgt ccttgcccga aatgaacggt gtgccaaaag
     1081 cagtggcata atcataacac gcccgggccg catccttgag ctgtctgagc cggacagggt
     1141 tcctggcgtc acaccagcag aaattatcga gcagggccag gtgttccaga gaccctcctg
     1201 ccgccacaac atgacggatg gccgtatcga tagcacaggc cgccatgcgg tacgcatcaa
     1261 catcagagta acggggattg attccctgag acaggaccac cccttttttg gaggagagca
     1321 caggacgcgt gatcgtggca ggcgcgcaca
//
LOCUS       DATDUN010000728         4002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_289279, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000728 DATDUN010000000
VERSION     DATDUN010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289279"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VJC18_10145"
     CDS             complement(<1..812)
                     /locus_tag="VJC18_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD45785.1"
                     /translation="MSNHDRNTLGVFENRWAIAVAGVVMQLVLGTVYAWSVFKKPFMA
                     AHGWSNEQVGLAFTLVILLIGVSAAFGGKFVDKAGARKIATLAAVLFGLGTILTGLAD
                     QMGSLAMLYIGYGLVAGIGNGLGYITPIAVLVRWFPDKRGLITGLAVMGFGFGAAIMG
                     QIAPFMIPKTEMVANVLTITEPGLGLSTTFYIFGAAFLIILLIAAQFLNNPPAGWAPA
                     SVAGKKQATTASVAVSVDLSGALRMPQFYILWTLLFINVTAGIALISNLSPMV"
     gene            complement(828..1472)
                     /locus_tag="VJC18_10150"
     CDS             complement(828..1472)
                     /locus_tag="VJC18_10150"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HLD45786.1"
                     /translation="MQSKYKGASIPLAVIERLAKYCLFAHRKNEEEVAWISSEALAKA
                     FGITDSTVRQDMSYVDFAGRAKCGYETQRMEQALRDTLGTNDMVNVGIVGAGNLGKAL
                     IMHSGFIRSRFNVRAIFDKDPSLVGKKAGSLKISSIKNLTRVVRDREIHIGVIAVPES
                     SAQDVADQLVAAGVKGLLNLAEMHLSVPENVRIQDVQIVANLMALRCQMEHSHS"
     gene            complement(1633..3105)
                     /locus_tag="VJC18_10155"
     CDS             complement(1633..3105)
                     /locus_tag="VJC18_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLD45787.1"
                     /translation="MTTATAAPFASPLTLPTITPELFAPPTNKPPAELAITRVLARLE
                     AERRDLGRDVISFGGGLPSPRTFDVRNYERHARAYFKHIAQRAQQGDIAAPTDPYKVW
                     QSVLNYTDTRAQWWIRNDFAEAHSRDMGFRIDPNRLVPTQGNTQGIHALLQYYNQLGV
                     PVAIFCERQAYTGMLTAAKQMPNLKIFSVDMDEDGMKPEHLIARQREAMAKGFIPGMI
                     YLVPDGGNPSGITISQQRRDDLYRQNLELARETGKIAYFFEDSPYYLIRVGSANTAYP
                     KPMAANDPAGVVIYAFSASKIGYPGKRVGWLHLPMAWCSTDAEGHNLTLHAEATAQIA
                     ATVLLHSSEALAEFNGQMFAPRTGAFVGLWPRSKVNSAIYRANMQMLYGGLATALAQP
                     HPELFKLSLMPSNGFVITLAFPGLASRTRFTNTSELATHLLEDSDVIVTPMAGFAPDD
                     IPPGSLDEIVRFTFSATPPERIDEGVERFVRGVKLAYNLP"
     gene            3350..>4002
                     /locus_tag="VJC18_10160"
     CDS             3350..>4002
                     /locus_tag="VJC18_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45788.1"
                     /translation="METATLTGLPTGSVRAPVLADVLDPNRGNWYGLKDAVGILSANQ
                     RVEAQKQKASAVRLSRFQGMEVLRARMEKRGIPIISHSLDADIFQSPDENRSIIELVC
                     NALDASKDGLDVHVNAGDGFFEVLDFGKGMNLQTLLYRLLMPKLSGKPNAEKTIGRFG
                     IGFYTALRHLKNEGESVVVETKTKRNAAMRVVITMRHGQLCVSDPVVLGSEEDFYSA"
BASE COUNT          965 a          979 c         1107 g          951 t
ORIGIN      
        1 accatgggag agaggttgct gatcagggca atcccggctg tgacgttgat gaagagcagt
       61 gtccagagaa tgtagaattg gggcatccga agggcaccgc tcaaatccac ggatactgcg
      121 acggacgcgg tcgtggcctg tttctttcca gccactgagg caggggccca tcccgccgga
      181 gggttattga gaaattgggc ggcgatcaac agaatgatca aaaaggccgc accaaagata
      241 taaaaggtgg tggagagtcc caaaccgggt tctgtgattg tgagcacatt ggcaaccatt
      301 tcggttttgg gaatcatgaa cggcgctatt tgtcccatga tggcggctcc aaaaccaaag
      361 cccatgaccg ctaacccggt aatcagaccg cgtttgtccg gaaaccagcg aaccagtacg
      421 gcaatgggtg tgatataacc caaaccattg ccgatccccg ctaccaaccc atagccgatg
      481 tagagcatgg ccagagagcc catctgatcc gccaggccgg tcagaatcgt gcctagtcca
      541 aatagcactg cggccagtgt ggcaatcttg cgggcaccag ctttgtcgac aaatttgccg
      601 ccaaaagccg cggatacccc gatgaggaga atgaccagtg tgaaggccag tcccacctgt
      661 tcgtttgacc atccatgggc cgccatgaag ggttttttaa agacgctcca ggcatagacc
      721 gtccccaaaa ccaactgcat gactacgcca gcgacggcga tggcccatcg attttcaaaa
      781 acacccaaag tatttcgatc atgattagac atgtgattga ctccttgtta cgagtgactg
      841 tgttccattt gacagcgaag tgccatcagg ttggctacaa tctgtacatc ctgtatacga
      901 acattttccg gcacactgag gtgcatctca gccagattca acaatccttt gacgcctgcc
      961 gcgaccagct gatcggcaac gtcttgggct gaagattccg gcacggcgat cacgccgata
     1021 tggatttcgc gatctctcac aacgcgcgtc aggtttttga ttgacgagat tttgagagaa
     1081 cctgcttttt tgcctaccag tgacgggtct ttgtcgaaga tagcccggac attgaaacgg
     1141 gaacggataa aaccggagtg catgatcagg gcctttccca gattgcctgc acccacaatg
     1201 ccaacattga ccatatcatt ggttcccagt gtgtcgcgta gagcttgttc catgcgctgg
     1261 gtttcgtagc cgcatttggc cctgcccgca aaatcgacat aggacatatc ctgacgaact
     1321 gtggagtctg tgatcccaaa ggctttggcc agcgcctctg aggaaatcca ggcaacctct
     1381 tcttcgtttt ttctgtgagc aaacaggcaa tactttgcca gtctctcgat caccgccagg
     1441 ggaatacttg cgcctttgta ttttgattgc attgtccaga ccagaaaacg acagatgaac
     1501 cgacactttc aggtcaatgt gtatcattaa tattcaaaaa cgcagaatcg ctacaacgtt
     1561 gcgggaaggg agtcaatcat tttatcgtca taattttaac ggttcatttg ggatgaaccg
     1621 gggatcaagg gttcagggca ggttgtacgc caatttgacg ccacggacaa agcgttcgac
     1681 cccttcgtca atgcgctccg ggggggtggc gctgaatgtg aatcggacaa tttcgtcaag
     1741 actgccggga gggatgtcat cgggagcaaa gcccgccatg ggggtcacga tgacatcaga
     1801 gtcttccaat aagtgagtcg ctagctcaga ggtgttggta aaacgtgtgc gactcgccaa
     1861 tccgggaaac gccagtgtga tgacaaaacc attggagggc atgagtgaca atttgaatag
     1921 ttctgggtgt ggctgggcca aagctgtggc caaaccgcca tacaacattt gcatgttggc
     1981 gcggtagatg gcgctgttca ctttagaacg gggccacaat cccacaaacg cacctgtacg
     2041 gggagcaaac atctggccgt tgaattcagc cagtgcctcg gaactgtgca aaagcacagt
     2101 ggcggcgatt tgcgctgtgg cctcggcgtg caaggtgagg ttgtgaccct cggcatcggt
     2161 ggaacaccag gccatgggca ggtgcaacca acccacacgt ttgccggggt agccaatctt
     2221 tgaggccgag aacgcgtaga tcaccacacc ggctgggtcg ttggccgcca tgggttttgg
     2281 ataagcagtg ttggcagagc caacgcggat gagataatac ggagagtctt caaaaaagta
     2341 cgcgatcttt cctgtctctc tggccaattc cagattttga cgatacaggt catcgcgtct
     2401 ttgttgtgaa atggtgatgc cactggggtt gccaccatcc gggaccaaat aaatcattcc
     2461 cgggataaaa ccttttgcca ttgcttccct ttgtctggca atgagatgtt ccggcttcat
     2521 gccgtcttca tccatatcca cactgaatat tttgaggttg ggcatctgtt tggccgctgt
     2581 caacatgcct gtgtaggctt gccgttcaca gaatatggct accggcaccc cgagctgatt
     2641 gtaatattgc aacagggcgt gaatgccctg cgtgttgccc tgcgtgggga cgagccggtt
     2701 gggatcgatt ctgaacccca tatcgcgaga atgggcctcg gcaaagtcgt ttcggatcca
     2761 ccattgggcg cgcgtgtcgg tgtaattcaa gacagactgc cataccttgt agggatcggt
     2821 tggagccgca atgtcacctt gttgtgcccg ctgggcaata tgtttaaaat aggcccgtgc
     2881 gtgtcgttcg taattgcgca catcaaaggt tctggggctg ggcagaccgc caccaaacga
     2941 aatcacatca cgtcccaggt cgcgtctctc ggcttcgagt cgtgccaaca cacgtgtgat
     3001 agccagttca gcgggcggtt tgtttgttgg gggtgcgaac aattctgggg tgatggtggg
     3061 cagtgtcaaa ggcgaagcaa aaggcgcggc agtggcagtg gtcatggttg tctcctcgat
     3121 gttatgtgcg ggcaccgtgg tgtggtcccg tcagaatatt aggacgcgcg tctcatagcc
     3181 agtttggggc acataattca agtaaaatgg caaggaaacc agggtttttt ttcgtttatt
     3241 ttgcacaaca tttcacaatg attgccgata ttagaaagcg gaggagtgtt atttggcgat
     3301 gaatggtcca ttaacaacgc tacccaatac tgcgttcaga attcctgcta tggaaacagc
     3361 gaccttgact ggtttgccaa cgggcagtgt gcgggcacct gtgttggctg atgtgttgga
     3421 tcctaaccgt ggtaattggt atggtcttaa agatgccgtc ggcattttat cggccaacca
     3481 gagggtcgag gcgcaaaagc aaaaagcatc tgctgtgagg ttgtcgcgat tccagggaat
     3541 ggaggtccta cgcgctcgaa tggagaaaag aggcattccc atcataagtc acagtctcga
     3601 tgccgatatt tttcagagtc cggacgaaaa tcgttccatc attgaactgg tgtgcaatgc
     3661 gctggatgca tccaaggatg gattggacgt gcatgtcaac gccggggatg gattttttga
     3721 agtgctggat tttggcaaag ggatgaattt gcagacactc ctttatcgcc tgctcatgcc
     3781 aaaactcagc ggcaaaccta acgcagaaaa aaccattggc cgctttggca ttggttttta
     3841 cacggctctg aggcacctga aaaacgaggg cgaaagcgtg gtggtcgaaa ccaaaaccaa
     3901 acgcaatgcg gccatgcgtg ttgtgattac catgagacac ggtcaactct gtgtcagtga
     3961 tccggttgtg cttggatccg aagaagactt ttattcggcc tt
//
LOCUS       DATDUN010000729         3654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_322604, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000729 DATDUN010000000
VERSION     DATDUN010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322604"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VJC18_10165"
     CDS             complement(<1..355)
                     /locus_tag="VJC18_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HLD45789.1"
                     /translation="MEFDRFVANLPLLTPLFVFAFLYVIATWRILSRAGLPGWGQFIP
                     IYNCLQILKLAGKEWWWIFLLCIPIVNFVIVVIVMIEIARNFGKGTGFGIGLCFLNPI
                     FWLILGLGSSPYQVPP"
     gene            complement(417..1361)
                     /locus_tag="VJC18_10170"
     CDS             complement(417..1361)
                     /locus_tag="VJC18_10170"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLD45790.1"
                     /translation="MSHIVNHVVAVSGDRIDHYLASQGLCTSRSEAQKLIKEGFVSVN
                     GEGIKAAYAVQADDRIVIDFPPVSPSQIVPHQVDFEILYEDHDIIVINKPAQLVVHPG
                     AGNYDTSLVHGLLHHCKDLSGIGGVERPGIVHRLDKGTSGVMVVAKHDRAHQNLSEQF
                     QKHLVVKEYLALCLGQILKPQQTIITFIKRSETNRKKFVVNQRKGKEAISHITVLRGN
                     ERVTLVSVKIDTGRTHQIRVHLSHLGHPVLGDEVYGGTKKLHLLKEQERQFVRGIGHP
                     LLHSRKLSLTHPVTAKLLVFEAKLPDDFIMAQNLFLIV"
     gene            complement(1414..2013)
                     /locus_tag="VJC18_10175"
     CDS             complement(1414..2013)
                     /locus_tag="VJC18_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLD45791.1"
                     /translation="MIDGPVLVLNRSFVPVHITSIKRAICMVFKGLATIVDEQYHCYD
                     FQSWAEMSVARADECIHLTKRLIRVPRVILLQFYDRFPKQDIKLNRENIYLRDANTCQ
                     YCHKKHRRSDLNLDHVIPVSQGGLTTWDNIVCSCLKCNHKKGGRTPEQAGMALTVKPA
                     KPKYSIFMNVSPQEQLFRAWHVYMNPVDYGYWCLELKEE"
     gene            complement(2096..2425)
                     /locus_tag="VJC18_10180"
     CDS             complement(2096..2425)
                     /locus_tag="VJC18_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45792.1"
                     /translation="MGIVVLSAFLICMGLLSIRFYFDQKDIHNAEGVFAARVVLQTPG
                     RQAFVNSLQCTKRLFSRYEGLVLFQCRDLQTGFVQETANNHYLWEADIYRNDIKPMNK
                     LAQTVMP"
     gene            complement(2451..2843)
                     /locus_tag="VJC18_10185"
     CDS             complement(2451..2843)
                     /locus_tag="VJC18_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45793.1"
                     /translation="MGKLAKYMFLGLLLFATLYFFGDFRVNDIHIRSYLQQHVTVDNI
                     LKAKQNCVKVVSALKRGLALVGNEVHKMDNPQRQVVADFKLDTTQDLKAQYDESLDSL
                     TAKDRLEMRSLIEKNVTNTDVAATTKQP"
     gene            2927..>3654
                     /locus_tag="VJC18_10190"
     CDS             2927..>3654
                     /locus_tag="VJC18_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLD45794.1"
                     /translation="MSPATYTSLITDETQSVPKILVVDDNETNVELICMHLKPYNYDI
                     RKAYDGDQAIKMIIQEEPDLILLDLMMPKISGYEVCQQIKSNPKTALIPIIIITALRE
                     LEDKIKAMELGADDFLIKPFNKLELLTRVKSLLRMKQLCDELDHGESVVFALAEALDA
                     KDIYTRGHSERVSKYSVILSRELKLSPVEIEEIKKGTLLHDIGKIGVRDSVLNKVDKL
                     TTEEIAHIRTHPARGYEICKPLKS"
BASE COUNT         1022 a          963 c          813 g          856 t
ORIGIN      
        1 ttggtggcac ctgatacggc gaggaaccca atcccagaat cagccaaaag atgggattca
       61 agaaacacag gcctattcca aaaccagtgc ccttgccaaa attccgggca atttcaatca
      121 tcacaatgac cacaatgaca aagttgacaa tgggaatgca caacaaaaaa atccaccacc
      181 actccttgcc tgccagtttc aatatctgga ggcaattgta gatgggaatg aactgacccc
      241 aacccggcaa acccgcacgg gacaggattc tccacgttgc aataacgtac aggaaagcaa
      301 aaacaaacag aggcgtcagc aacggcaaat tcgctacaaa tcgatcaaat tccataactc
      361 ccccttttat aaatagtgtt taacaaaccc gcccaagttt aacaaatcag ccccagtcaa
      421 acaatcaaaa aaagattctg tgccataata aaatcatcag gcaatttagc ctcgaacacc
      481 aggagttttg cagtcacagg atgagtcaag gaaagtttac gggagtgaag aagaggatga
      541 ccaataccgc ggacaaactg cctttcctgt tccttcaaca gatgaagttt ttttgttccc
      601 ccataaacct catctcccag aacaggatgc cccaaatgac tcaggtgcac gcggatctgg
      661 tgcgtgcggc ccgtgtcgat cttgacagac accagcgtca ctcgctcatt gcccctcagc
      721 acggtaatgt ggctgatggc ctccttgcct tttctctggt tcaccacaaa ttttttacga
      781 ttagtctctg accgtttaat gaaggttatg attgtctgtt gcggttttaa aatttgaccc
      841 aaacacaaag ccaggtattc ctttaccacc agatgctttt gaaactgttc tgataaattc
      901 tgatgcgccc tgtcatgttt ggccaccacc atcaccccgg atgttccctt atccagccga
      961 tgaacaatcc ccggtcgctc cacaccgccg atgcctgaca aatccttgca atgatgcaat
     1021 agcccatgca ccaagctggt gtcgtaattg cccgctcccg gatgcaccac caattgagcc
     1081 ggtttgttga tgacgatgat atcgtgatcc tcataaagaa tctcaaaatc cacctgatgc
     1141 gggacaattt gggaagggct cactggtgga aaatcgatga cgatgcgatc gtctgcctgt
     1201 acggcataag ccgccttgat cccctccccg ttcaccgaga caaagccttc tttgatgagt
     1261 ttttgcgcct cggagcgcga ggtacacagc ccctgactgg ccagataatg atcaatgcga
     1321 tctccggaaa cagcaacaac atgattgact atgtgagaca cgaaaattgc ttaacgtacg
     1381 cacgcacgca cgtcaacacg tacgcacgga aaatcattct tccttcaact ccagacacca
     1441 gtagccgtag tctaccggat tcatgtagac gtgccaggcc ctgaagagtt gttcttgagg
     1501 tgagacgttc atgaaaatgc tgtacttggg tttcgccggt ttgacggtca acgccattcc
     1561 cgcctgctcg ggtgtgcgac cacccttttt gtgattgcac ttgaggcaac tgcacacaat
     1621 gttgtcccaa gtggttagtc cgccttgaga cacgggaatc acatgatcca aattaagatc
     1681 tgaccggcga tgttttttgt gacaatactg gcaggtgttc gcgtcgcgaa gataaatatt
     1741 ctcgcgattt aatttgatgt cttgcttggg aaagcggtcg taaaactgta gcaagatcac
     1801 ccggggcacg cgaatgagtc tcttggtcaa atgaatgcac tcgtcggccc gtgccaccga
     1861 catctccgcc caggactgaa aatcgtaaca atgatattgt tcatcaacga tggtggccaa
     1921 ccctttgaaa accatgcaga tagcccgctt aatggaagtg atgtgaacgg gcacaaagga
     1981 tctgttgaga acaagaaccg ggccatcgat catacctcta ttttacagca attcaaggct
     2041 cttgaaaaga gccatttttt ctgcgcagaa aactccggtt aacaacgtca ggcaactatg
     2101 gcatgacagt ttgagccagc ttgttcatgg gcttgatatc atttcggtaa atgtcggcct
     2161 cccacaaata atgattattg gcagtttctt gaacaaagcc tgtctgcaag tcgcgacact
     2221 ggaataaaac gaggccctcg tagcgggaaa aaagtctctt ggtacattgc agtgaattga
     2281 caaaggcctg ccgtccggga gtctgcaaca caaccctcgc cgcaaaaaca ccctcggcat
     2341 tatgaatgtc tttttgatcg aaataaaaac gaatactgag cagtcccatg cagatgagaa
     2401 aagccgacaa gactacaatg cccattcgat aaatcatgga tgatgaggag tcagggctgc
     2461 ttggtcgtcg cggccacatc ggtattcgtg acgtttttct cgatgagcga ccgcatctcg
     2521 agtctgtcct tcgcggtcaa ggaatcgagg gactcatcat attgcgcctt gaggtcctgc
     2581 gtggtgtcta gcttaaagtc ggccacaacc tggcgttgag gattgtccat tttgtggacc
     2641 tcgttaccca ccagagccaa acctctcttg agggcgctga caaccttcac gcaattttgc
     2701 ttggccttga ggatattatc gacagtcaca tgctgctgga gataactgcg gatgtgaatg
     2761 tcgttcacgc gaaagtcacc aaaaaagtac aacgtggcaa aaagaagcaa acccagaaac
     2821 atgtatttgg ctaattttcc catacgtcat cataaacgca acagcaccga tcgtgcaatc
     2881 taaaaattga ttgtgatttc atgggtccta tggtctaatg gttgcaatga gcccagccac
     2941 ctacacatca ctcatcaccg acgaaaccca gtccgtccca aaaatcctgg ttgtggacga
     3001 caacgaaacc aatgtggaac tcatctgcat gcaccttaag ccttacaatt acgacatccg
     3061 taaggcttat gacggcgacc aagccatcaa aatgattatc caggaagagc ccgatctcat
     3121 tttactcgac ctcatgatgc ccaagatatc gggttatgaa gtctgtcaac agatcaagtc
     3181 caaccccaaa accgcactga tccccatcat catcatcacg gcactgcggg aactcgaaga
     3241 caagatcaag gccatggagt tgggggccga tgattttttg atcaaacctt ttaacaaact
     3301 ggaattgctc acgcgcgtta aatcgctcct gcgcatgaaa cagctctgcg atgagctcga
     3361 tcacggtgag tctgtggtgt ttgccttagc cgaggcactc gacgccaaag acatttatac
     3421 gcgcgggcat tccgaacgcg tctcgaaata ctccgtcatc ctgtcacgcg aactcaaatt
     3481 gtctccggtt gaaatcgaag agatcaaaaa aggcaccctg ctacacgaca ttggcaaaat
     3541 cggtgtgcgc gattccgtgc tcaacaaggt cgacaaactc accaccgagg agatcgccca
     3601 catccgcacg cacccggcca ggggttacga gatctgcaaa cccctcaagt catt
//
LOCUS       DATDUN010000730         1540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_78867, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000730 DATDUN010000000
VERSION     DATDUN010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78867"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VJC18_10195"
     CDS             complement(<1..440)
                     /locus_tag="VJC18_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HLD45795.1"
                     /translation="MKFELNQLISKYADVVLPLLVVSVFAMIIVPMPTWLLDILLVMN
                     ISLGMIILLVAMYITDALKIASFPTILLITTLFRLALNISSTRLILLNANAGEVILAF
                     GNFVAGGNVVVGGILFLIITIVNFIVIAKGAERVSEVAARFTLDA"
     gene            713..>1540
                     /locus_tag="VJC18_10200"
     CDS             713..>1540
                     /locus_tag="VJC18_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HLD45796.1"
                     /translation="MPHLSLEHYFTHPIYLMFASIAALFLGPLINAMFRGQRFVRDTI
                     EGFTLVMIILLVAMYILPHTVHDVGFWAVVMLLIGMFLPSVFEKTYVRLASHVHYATI
                     FVVMLGFATHAFLDGVALVTPSSICSIDAEHSVSMLPIAVLLHRLPEGLAIWTLIRPW
                     YGNRRAILMLLISAISTVAGVTSGSLLISHIPHEPFGFFEALIAGLLLHILFHRHDPY
                     EPSGCHDCETPKFDFKWGTGVGAILGFVLILAIQSSQHADHAHGSKAMIFWNLAFSSA
                     "
BASE COUNT          369 a          425 c          336 g          410 t
ORIGIN      
        1 gcatccaatg taaatcttgc ggcaacttcg gaaacacgct cggcaccttt ggcgataacg
       61 atgaagttca cgatcgtgat gatgagaaac aaaatgccac ccaccaccac gttacctcca
      121 gccacgaaat taccgaacgc caagatcacc tctccagcgt tcgcgttgag cagaatcaaa
      181 cgagttgagg atatgttaag ggccagtcgg aacagcgtgg tgatcaacaa gatggtagga
      241 aacgacgcaa tcttgagcgc gtcggtgatg tacatggcca ccagcaaaat gatcatcccc
      301 aagctgatgt tcatcaccag caaaatatcg agtagccacg tgggcatggg aacgatgatc
      361 atggcgaaca cgctcacgac cagcagaggc aacaccacat cggcgtactt ggagatcagt
      421 tggttgagtt caaatttcat ataatactat taccagttta acattgtttt gccctgtgca
      481 ccaaacactt attttcttga acaaacaacc acaatgaccc caactcaaaa acaccgccta
      541 acaaattgta attatatatc attttatttt ggttgccaca ataataatgg tccctcacaa
      601 ggagtatcgg cctgttttca tccaaagttg gggttttaat gattatttac gctctgaaga
      661 tattttttgt cgctggtaat atggtggcca atacgataca cttatcgacg acatgcccca
      721 tttatcactt gaacattatt tcacacaccc catttacctc atgtttgcca gcatcgccgc
      781 tctgtttttg ggccccctga tcaacgcaat gttccggggg caaaggttcg tgcgtgatac
      841 gatcgaaggc ttcacactgg tcatgatcat tctgctggtt gccatgtaca tcctgccgca
      901 cacagtgcac gatgtgggct tctgggccgt cgtcatgctc ctcatcggca tgtttctgcc
      961 ctccgtattc gaaaaaacct acgtgcgcct cgcctcacat gtccactacg ccacgatctt
     1021 tgttgtgatg ctcggtttcg ccacccacgc ttttttggac ggtgtggcac tggtcacccc
     1081 cagctcaatc tgttcgatcg acgcagagca ctcagtcagc atgttgccca tcgcagtcct
     1141 tttgcaccgc cttcccgaag ggctggccat ttggaccttg atcagaccct ggtatggcaa
     1201 ccgccgggcg atcctcatgc tgttgatttc tgccatctca actgttgccg gggtcacctc
     1261 gggatcattg ttgatctcac atatccctca tgagcctttt ggtttcttcg aagccctgat
     1321 cgccggacta ctgttgcaca tcctctttca tcgccacgat ccttacgagc caagcggctg
     1381 tcatgactgt gaaactccaa aattcgactt caaatgggga actggcgttg gtgctatcct
     1441 gggttttgtt ttgatcttgg ccattcagtc ttcccaacac gccgaccatg cccacggatc
     1501 aaaggccatg atcttctgga atctggcttt ttccagcgcg
//
LOCUS       DATDUN010000731         4454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_289356, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000731 DATDUN010000000
VERSION     DATDUN010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289356"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VJC18_10205"
     CDS             complement(<1..1076)
                     /locus_tag="VJC18_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45797.1"
                     /translation="MTEYRINESIVPENVFSSLKSEINNIYNKVLAGVDQETALFTTL
                     DCFANDNNISFDELRLLYELTASIPSSHKKQFDGYAFKWFGLTPNVAFNTPVTTSTLD
                     GQHYQINGVTLPDTIWNSREYVENHLRSAFLATPEDIAEVGKDNNLTSDDIKYLTDRN
                     HDDLLDAYEISLTTTQDGLPLGIGNPFLTGLDVFGINVVEMYSEDNTSRPTTSASAAT
                     SKDKALFAIRQSGLNIAGMTTEEKRDFDIARAAVLNDERAIDHIDPNLLRNPLFLCTL
                     KGYYLAPYVYQRIVETLKDEPLVLQELTLHLPLVFAYLPEDMRASKPFVVEYLTSITL
                     PFRDDINAVAPHIAESLRNDRDIAL"
     gene            complement(1103..3892)
                     /locus_tag="VJC18_10210"
     CDS             complement(1103..3892)
                     /locus_tag="VJC18_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HLD45798.1"
                     /translation="MGSNIINTIRKAEETLGIGYFINGVKVSEELFDKVKDDFAREFG
                     EITTTTTTDSSSTDNTTNNEKVVYAQGMAEMFVDNRLTREEIQDFYAATRFDSKCYRA
                     SHHILNTPPFDSYAYKLFGLEFDGGYNQPRTTELSTQDHYKINGVDLPDTFWNSRMYI
                     DNHLRAIFLSGTDEPSAANKPSAADESSAAAEPSATEKLSAVGHDLNLTTNDIATLMD
                     TNNDGAVDRHEFDAIKNSRDLSAQGWDSFIRGLSYFGIDIDAIHEKSMKKNPEKIDAS
                     ANSKEKAMAENNDDWENFQYSSPVERGDYEIARDAVVANKKNIDHVNRELVSDIRFLS
                     YLAKSLRGIHDEERRHIYERLVSLSSSDTAFLQNLAIQWPDVFIFLPEPLRNSKTFVS
                     EFLTGVQLANEEGELLTDIINSIGPELKSDKDTAMLAVTVNGRALEQLKQFAGYKPVV
                     LRAMTSYGAALQWASTTLKNDRDVVLASANTQDTELGASRTLELANPVFKSDKEVVLA
                     VLKNSQRPEIKAQLNHNPFSYVGDTLKKDREVVLEAVRVKGDNLFFAGVSDHFRNDRQ
                     IVFEAVCANPEILTSDSLIKYRTERNFLLEALARNPDVWPFLDSDLQVTKERVAEAVN
                     QGVSLEQVRSDLLNEKELVSSAMEKHSDYVDEYLPIFNDNAELMEQHLRSRPYWNGTK
                     ADFALLSKEHKILALQRFGEQIYPEQLNDDVVDINEKGATRYIERDDRELIAAAAKSM
                     GTKIFYLMPEKFMDDKTIIDAALNGPVTSNALALFSEEQRKQTSFVVPFVSRNGLMLK
                     YADDSLKANRDVVMTAIQSNGFALTYASESLRKDPELIRLANQQMGFEYFKGPAKAPV
                     AVPAVISTPVNAEEELESVAISMEPVVEVPLEPEPTPTPKSVPVLTQAQAQTQTQPKP
                     KASKPKSDAISDEDY"
     gene            complement(3905..>4454)
                     /locus_tag="VJC18_10215"
     CDS             complement(3905..>4454)
                     /locus_tag="VJC18_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45799.1"
                     /translation="ILSAFSDFCVTSEEIESIFDDPQAIDPASHTHKQISLPEKDSYA
                     MGLLGLDPFFDVDRFIHDQQQDFSFELNGNKIFSKSARVREQFEASLGTMVTAKLFDI
                     KVALSDHCLTSAELFALWDANGNKIIDDTEELFLRTQPSNTDQQTTRPFSSAAFNESM
                     KWFGLDLDSLKEQFCNRKKIHI"
BASE COUNT         1034 a          996 c         1130 g         1294 t
ORIGIN      
        1 agggcaatat cccgatcgtt tctgagtgac tcggcaatgt ggggtgctac ggcgttgatg
       61 tcgtcccgga atggcagagt gatgctggtg aggtattcca cgacaaacgg ctttgaggcc
      121 ctcatgtcct ctggcaaata ggcgaataca agtggcaggt gcaaggtcaa ttcctgcaag
      181 accaatggtt catccttgag tgtctccaca atgcgttggt acacataagg tgcaagataa
      241 tatcctttca gcgtacaaag gaatagtgga ttgcgaagga gatttgggtc aatgtgatcg
      301 atggctcttt cgtcattcag aacagcggct ctggcgatat caaagtcgcg tttttcttcc
      361 gtagtcatgc cggcaatatt gaggccactc tgtcgtatcg cgaacagagc cttgtctttg
      421 ctggttgcgg ctgatgcgct ggtagtcggt cgcgaagtgt tgtcttcgga atacatttct
      481 acaacattaa tcccaaaaac atcgagccct gtcagaaaag gattgccgat tcccaaaggc
      541 aatccatcct gcgttgtggt gagagaaatt tcataggcat caagcaagtc gtcgtgattt
      601 ctgtctgtca aatatttgat gtcgtcactg gtgaggttgt tgtctttacc cacttccgca
      661 atatcttctg gtgtcgccaa aaaggcagaa cgcagatggt tctctacata ctctcgtgaa
      721 ttccagatgg tgtcaggcag ggtcacgccg ttgatctggt aatgttgacc atcaagggtg
      781 ctggtggtga ctggtgtgtt gaacgcaaca tttggagtca agccaaacca cttgaaggca
      841 taaccatcaa actgcttttt atgactggaa gggatgcttg ctgtgagttc atagagcagg
      901 cgtagttcat caaaagagat gttgttgtca ttagcaaaac agtccagtgt ggtgaagaga
      961 gccgtttcct gatcaacgcc ggccagaact ttattgtaaa tattatttat ctcagacttc
     1021 aaactgctga atacattttc gggtacgatc gattcgttga tgcggtattc tgtcataatg
     1081 actccaatta atcacaatcg tcttagtagt cctcatcact gatcgcgtcc gattttggtt
     1141 tggacgcctt tggttttggt tgtgtctgtg tctgtgcctg tgcttgggtt agaactggaa
     1201 cagattttgg cgtgggtgta ggttcaggct ctagaggaac ttccaccaca ggttccatgg
     1261 agatggccac actttcgagt tcttcttctg cgttgaccgg cgtggatatc acagcgggca
     1321 cggcgacagg tgctttggca ggccccttaa agtattcaaa tcccatttgc tggtttgcga
     1381 gtcgtatcaa ctctgggtct tttctgaggc tctcgctggc atacgtcagc gcgaacccgt
     1441 ttgattggat cgccgtcata accacatcgc gattggcctt gagactgtca tctgcgtatt
     1501 tgagcatcag accgtttcgt gagacaaagg ggacgacaaa actggtctgc tttctttgtt
     1561 cctcagagaa cagggcgagg gcattgctgg ttacggggcc attaagagcc gcatctataa
     1621 tggttttgtc atccataaat ttttctggca ttaaataaaa gattttggtc cccatggatt
     1681 tggccgccgc ggcgatcaat tctctgtcgt ctcgttctat gtaacgcgtg gcgccttttt
     1741 catttatgtc aacaacatca tcatttagct gttctggata gatctgttca ccaaatctct
     1801 gcagggccag tattttgtgc tctttgctga gtagcgcaaa atcggccttt gtgccgttcc
     1861 aataggggcg agaccggagg tgctgttcca tgagctcagc gttgtcattg aatatgggca
     1921 ggtactcatc gacataatca gagtgttttt ccatggcaga gcttaccaat tctttttcgt
     1981 tcaagagatc tgagcgaact tgctccagag agactccctg attcaccgct tctgcgacgc
     2041 gttctttggt cacctgaaga tcagaatcaa gaaagggcca cacatccgga ttgcgggcca
     2101 gggcctccag taaaaagttt ctttccgtgc ggtatttaat caggctgtca ctggtcagga
     2161 tttctggatt ggcacagact gcctcaaata caatttgtcg atcatttcta aaatgatctg
     2221 atacgcctgc aaaaaacagg ttgtcgcctt taacgcggac ggcctcgagc acaacctcac
     2281 gatctttttt cagagtgtca ccaacataag agaagggatt atgattgagc tgtgctttga
     2341 tctctggcct ctgagaattt ttaagcacag ccaagaccac ttccttgtca ctcttgaata
     2401 caggatttgc caattcgagt gttctggagg cccccaattc tgtgtcttga gtgttggcac
     2461 tggcgagcac cacatctcga tcgtttttga gcgtggtaga agcccattgc agtgccgcgc
     2521 cataactggt catggcccgc aatacgacgg gtttgtatcc agcaaattgt ttgagttgtt
     2581 ccagggcccg accgttcacc gtgaccgcca gcatagcggt gtctttatct gattttagtt
     2641 cgggcccaat gctattgatg atgtcggtca gtaactctcc ctcctcattt gccagctgga
     2701 caccggtcag gaactccgac acaaacgttt ttgaatttct gagaggctct ggtaaaaaga
     2761 tgaatacatc gggccattga atggccagat tttgtaaaaa agccgtgtcc gaagaagaga
     2821 ggctgaccag ccgttcatag atatggcgac gctcttcatc atggatgccg cgtaaagact
     2881 tggctaaata gctgagaaat ctgatgtcgc tcactagttc gcgattgacg tgatcgatat
     2941 ttttcttgtt tgccacaaca gcatcccgcg cgatttcata atcgccacgt tcgacgggac
     3001 tgctatactg aaaattctcc cagtcatcat tattctctgc catcgccttt tccttgctgt
     3061 tggccgaggc atctatcttt tcgggatttt ttttcatgga cttttcatgg atggcatcaa
     3121 tgtcaattcc gaagtaggac aaccctctga tgaaagagtc ccatccctga gcggagaggt
     3181 cacgactgtt tttgatagcg tcgaactcat gtcgatcgac agcaccgtca ttgttggtgt
     3241 ccatcagtgt ggcaatgtca ttggttgtca gattcaggtc gtgtccgact gcggaaagtt
     3301 tttcggtagc ggaaggttcg gcggcggcgg aagattcgtc ggcggcggaa ggcttgttag
     3361 cggcagaagg ttcgtcggta ccagacaaaa aaatggcacg aaggtggttg tcgatgtaca
     3421 tgcgggagtt ccaaaaggta tcgggcaaat caaccccgtt gattttatag tgatcctgag
     3481 tgctgagttc tgttgtgcgg ggttgattgt aaccgccgtc aaattccagg ccaaatagct
     3541 tgtaggcata ggagtcaaat gggggcgtgt tcaaaatgtg gtgagaggca cggtaacact
     3601 tggagtcgaa tcgcgttgca gcgtaaaaat cttgaatttc ctcacgtgtg agccggttgt
     3661 cgacgaacat ttccgccatg ccttgcgcgt agactacctt ttcgttgttg gtcgtattat
     3721 ccgtgctcga gctatcggtg gtcgtggtgg ttgtgatttc accaaattcc ctggcaaaat
     3781 catctttaac cttatcaaac agttcctctg acacttttac gccattgatg aagtaaccaa
     3841 tccccaaggt ctcttcggct tttctgattg tgtttatgat gttgcttccc ataaaatccc
     3901 ctgattagat gtgaatcttt tttctgttgc aaaattgttc tttgagagag tccaaatcca
     3961 gtccaaacca tttcattgat tcgttgaatg cggctgaaga aaaaggtctt gttgtctgct
     4021 ggtctgtgtt tgatggctgt gtcctgagga acagctcctc ggtgtcatca atgattttat
     4081 tgccgttggc gtcccacaac gcgaacagct cggcgcttgt gaggcagtga tcagataagg
     4141 cgactttgat atcaaataat ttggctgtga ccatggtgcc gaggctggct tcgaattgtt
     4201 cacgaactct ggcagacttt gaaaatatct tgttgccgtt caattcaaag ctgaaatcct
     4261 gttgttgatc atgaatgaaa cgatcgacat caaagaaggg gtcgagtccc aaaaggccca
     4321 tggcatagct gtccttctcg ggcaggctga tttgtttgtg cgtgtgtgat gcggggtcta
     4381 ttgcttgagg atcatcaaaa atgctttcga tttcttcact cgtcacacaa aaatcagaaa
     4441 acgcggagag gatg
//
LOCUS       DATDUN010000732         1400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_370750, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000732 DATDUN010000000
VERSION     DATDUN010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370750"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VJC18_10220"
     CDS             complement(<1..225)
                     /locus_tag="VJC18_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45800.1"
                     /translation="MWSSPPAHQRAWHGQTKAQLIKPLIVFFLLIIPLKSQAGDDSLK
                     ATIDSFSRPNTSIGVMVHDIKTTKELFSHRG"
     gene            complement(326..991)
                     /locus_tag="VJC18_10225"
     CDS             complement(326..991)
                     /locus_tag="VJC18_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HLD45801.1"
                     /translation="MQQPKKLFFLFLILLFIPPSLRAEPSLAQRVESRYQNTRTWQAS
                     FVQTTFVDILKQKIKKSGMILVKKPDQLRIEYTSAPNKTYITDGHKLWIYKKDSLTAQ
                     QFGKPKDIISAEALSFMGGLKNLSQLFETLPPLNEPKVFVSIQNKSLQKIHLTPKDKN
                     SAILKLTLGVDSRDLSIREAVIFNTSGNVTHYAFNNLIFDKDLADSEFALPPSPKRNI
                     IKQ"
     gene            complement(991..>1400)
                     /locus_tag="VJC18_10230"
     CDS             complement(991..>1400)
                     /locus_tag="VJC18_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLD45802.1"
                     /translation="GFAISQAHNCQLLEKFLAPNRLSRITCAIVDEVFKSHHYVYENA
                     ETLVVERGRMFSKMQTIEGVRVFPSEANFLLFRMDKAHEVARLLQNDGFLIRNVDNQT
                     SLINCLRVTIGSPEQNDAFLLTLKKVKNEVKDL"
BASE COUNT          327 a          296 c          384 g          393 t
ORIGIN      
        1 gccgcgatgc gaaaacagct ctttggtcgt tttgatgtcg tgcaccatca cgccgataga
       61 ggtgtttggg cgcgaaaagg aatcaatcgt ggctttgagc gagtcatcgc cggcctggga
      121 ttttaaggga atgatcaaca aaaagaaaac gatcagaggc ttgatcagct gtgcctttgt
      181 ctgcccgtgc caagcccgct ggtgggcagg tggcgagctc cacaagcaat tgatttctgg
      241 agattgcttc ggcctttcct gcctcgcaat gacaacccct attttattga aaaaacaact
      301 tttgtgcata ggtcagtttt ccctgttatt gtttgatgat atttctcttt gggctggggg
      361 gcagggcgaa ttccgaatca gccaggtctt tatcaaaaat caaattgtta aaggcgtaat
      421 gggtgacgtt gccggaggtg ttgaagatca cagcctcccg gatggagagg tcccgcgaat
      481 caactcccaa ggtcaatttg agaatcgcgg aattcttgtc tttgggggtg aggtgaattt
      541 tttgcaggga tttgttttga atcgacacaa aaactttcgg ttcattcagt gggggcaagg
      601 tttcgaaaag ttgggacaga ttttttaacc cccccatgaa actcagggcc tcggcactga
      661 tgatgtcttt gggtttacca aactgctgtg cggtcaagct gtctttttta tagatccaaa
      721 gtttatgccc gtcggtgatg taggttttat ttggcgcaga ggtgtattcg atgcgaagct
      781 ggtctggttt tttgaccaaa atcatgcccg attttttgat cttttgtttg aggatgtcca
      841 cgaacgtcgt ttgtacaaaa ctggcctgcc aggtgcgcgt gttctggtaa cgggattcaa
      901 cccgctgggc cagtgagggc tcggcgcgca gagatggggg gataaacagc aatatcagaa
      961 aaagaaaaaa gagttttttg ggttgttgca tcagaggtcc tttacttcat tttttacctt
     1021 tttcagggtc aacaaaaagg cgtcattttg ctcagggcta ccgatcgtga ccctgaggca
     1081 gttgatcaga gaggtttggt tgtcgacgtt tctgatcagg aaaccgtcgt tttgcaaaag
     1141 tctggcgacc tcatgggcct tgtccattcg gaagagtaaa aaattggcct cggacggaaa
     1201 caccctgacc ccttcaatcg tctgcatttt ggagaacatc cgcccgcgtt caaccacgag
     1261 ggtttcggcg ttttcgtaaa cgtaatggtg acttttaaaa acctcatcca caattgcgca
     1321 ggtgatgcgc gacaggcggt tgggggccag aaatttttcg agcagttggc agttgtgggc
     1381 ctgactgatg gcaaacccga
//
LOCUS       DATDUN010000733         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_446893, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000733 DATDUN010000000
VERSION     DATDUN010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446893"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VJC18_10235"
     CDS             <1..353
                     /locus_tag="VJC18_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45803.1"
                     /translation="GIVITCLDRLNDVADWKMVTSYHAQWWDPDLDLLDRDTHICQYE
                     RLVPGPFKDLEYQSRLTSLLGRVEPVLETTTRASDFETRAKEHVERIERELALPVHML
                     SFGPTANDKRFLKE"
     gene            complement(373..1563)
                     /locus_tag="VJC18_10240"
     CDS             complement(373..1563)
                     /locus_tag="VJC18_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLD45804.1"
                     /translation="MDNGAIIREWLLFVKTDQRNPCYSRTTMPGRNVHMTLEKWFFLV
                     LSLFVLSLFWTIMEPFAFVLITALITAIVISPLERSLNRFLGHKHISAAIMTVGVLML
                     VFVPLILLVTVMIGQAKDILAQPFADQGWIEGTRTVLHSLLFFLPEQIQTYFINYDLG
                     ALQSKAAQWTFNNLGSVFASTTEGILNTFLYFLSLYYLLVDRDLLVNKLVSLSPLRDS
                     LDLDLIRRLIVTVRSVVFGVLLIGILQGILAAIGMTIFGVPGALIWGAITIIASLVPF
                     VGTALVLIPAILYLFFTGSQTAALGLLIWSVVCVGLADNFVGPFLMKGTTNMNALLIL
                     LSILGGLQTFGPIGIIVGPTILAGFLALIELYTSGVFHNDRLLKQKRQTDPSLGRDDT
                     RKRK"
     gene            1569..1643
                     /locus_tag="VJC18_10245"
     tRNA            1569..1643
                     /locus_tag="VJC18_10245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1602..1604,aa:Glu,seq:ttc)
BASE COUNT          702 a          567 c          646 g          591 t
ORIGIN      
        1 atggaatcgt gatcacgtgc ttggatcgtc tcaatgacgt tgctgattgg aagatggtta
       61 cgtcttatca tgcgcagtgg tgggatcctg atctggattt gcttgatcga gatacccata
      121 tttgtcagta cgagcgtttg gttccgggtc cgttcaagga tcttgagtac cagagtcgcc
      181 tgacgagttt gcttggtcgc gtcgaaccag tccttgaaac aacaacacgc gcctcggatt
      241 tcgagacgcg tgccaaggaa catgttgaga ggattgagcg tgagttggca cttccagtgc
      301 acatgctctc gtttggcccc accgcaaacg acaaacgatt tctcaaagag taatcacgac
      361 gattgctctt ttttattttc ttttccttgt gtcatcccga cccaaggagg gatctgtttg
      421 tcttttttgt ttcagtagtc tgtcgttgtg gaacacacca gatgtataca gttcgatcag
      481 agcaaggaat cctgccagga tggttgggcc aacaatgatt ccaattggac caaaggtttg
      541 taatccacca agaatggaaa gcaagataag cagagcgttc atgttggttg tccctttcat
      601 aagaaacggc cccacaaagt tgtcggccaa accaacacag acgaccgacc agatgagcaa
      661 tccaagcgcc gcggtttgtg atccagtaaa aaagagatag agaatggcgg ggatcagcac
      721 aagggcggtt ccaacaaacg ggacaagtga ggcgatgatc gtgatcgctc cccaaataag
      781 agcgcctggt acgccaaaga tcgtcatgcc aatggcagca agaatgcctt ggagaatacc
      841 aatgagcaga acaccaaaga caaccgagcg aaccgttacg attaatcgac gaatcagatc
      901 aaggtcaagc gagtctcgaa ggggagacag ggagacgagt ttgttaacca aaaggtctcg
      961 atcaacaaga agatagtaga gggagagaaa ataaaggaag gtgttcaaga taccttctgt
     1021 ggtacttgca aaaacggatc caagattatt aaaggtccac tgtgccgctt tgctttgaag
     1081 cgcccccaaa tcgtagttta taaaataggt ttgaatttgt tctgggagaa aaaagagcag
     1141 ggagtgcaaa acggtccgcg tgccctcgat ccatccttga tcggcaaatg gctgagcaag
     1201 aatatccttg gcctgtccga tcatgacggt tacaagtaag attaagggaa caaaaaccaa
     1261 catcagcaca cccaccgtca tgatggctgc agaaatgtgt ttgtgaccta aaaagcgatt
     1321 gagactccgc tcaagcggtg agataacaat ggccgtaata agcgctgtga taaggacaaa
     1381 cgcaaacggc tccatgatgg tccaaaataa tgaaaggaca aaaagagaaa ggacaagaaa
     1441 aaaccatttc tcaagtgtca tgtgaacatt cctccctggc atagtagttc tactataaca
     1501 tgggttcctc tgatctgtct tgacgaatag gagccattcc cgtatgatag cgccgttatc
     1561 catgagacgg ccccatcgtc tagtggttag gacggcaggt tttcatcctg caaatcgggg
     1621 ttcaattccc cgtggggtca ccacaaaaaa cactcagctt ttgctgggtg ttttttgttg
     1681 gtgtgtgggc ccccacgggg aattgaacgg aggatggtaa gggaggagaa ttcattacag
     1741 acaaggatgt cattcatgtc ttcatcccaa cttgccgatt taggccagac tggagccatc
     1801 gatttgtatg aaaacaaaaa acctccgttg tcacggaagg tcagatgaaa tcaatagttc
     1861 gagtctatgc aggatcagca atggcgagca gtgcggtcgt gtgggtttgc agctttactg
     1921 ctcctccacg cgggagacgt ttgtcagccg ttgtagatga tgctagttga acaaactgga
     1981 accttcgacc tggaaactgc ttatacagtc gttgaataca accagccaac agctctgaag
     2041 gatggcgtgc tgttgtggga tgatatccac cctcggacac accgagcatg acggtcagag
     2101 gatcgatatt taccacgaga ataggcggcc cttcacagga agttgagtgg tccttgagat
     2161 gttcgaatcg atctccacaa aacggacatg tcaccgccat aaaagtctcc acatttgagc
     2221 ctggtatagg ccaagaaaac ctacataact atcttgagag tgtcaatccc gtgttctctc
     2281 taaaaacacc ccggccgcgc acgttttgcg ggtcggggtt ttggtcgagc gggtcttcaa
     2341 cgacgcagat tcatttctcc tttggaagca ggtactcggg aaatcgagca gagacgtttg
     2401 acgttcaggc caaatcgtcg agacgaagca tcacgtccat agagacactc gtattggata
     2461 tccagagtgt gcgggacagc ctccatgagc tcgttgtagc ccccgt
//
LOCUS       DATDUN010000734         3974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_405216, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000734 DATDUN010000000
VERSION     DATDUN010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405216"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VJC18_10250"
     CDS             complement(<1..548)
                     /locus_tag="VJC18_10250"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HLD45805.1"
                     /translation="MTFHFSRNDCLNLESSLRCEWLETNGLGGYASTTIVGCHTRKYH
                     GLLVATLPEHNAKFVLLSKMGGAIRVGGYVFNLSTNKFPGVFHPTGHKYIESFEYDTH
                     PKTVYRIGDIRFVRELIMPQNENTVILKYQLMASQVPITFHLNPRLAFRQIHELAKDN
                     IDLRVKTYVMDDNIYKIQPYDSL"
     gene            complement(684..899)
                     /locus_tag="VJC18_10255"
     CDS             complement(684..899)
                     /locus_tag="VJC18_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45806.1"
                     /translation="MLKNKNTKKKVIERFPRKELNAWLKGRECWDHDEWLDLLDDLRR
                     QGFTKWTESQDGQNEIGSYLEESRVTA"
     gene            complement(985..1731)
                     /gene="recO"
                     /locus_tag="VJC18_10260"
     CDS             complement(985..1731)
                     /gene="recO"
                     /locus_tag="VJC18_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HLD45807.1"
                     /translation="MTRDTDQQATRGIVLRKIPYGEADEIVTCLLQKIGVVTFFASGS
                     RKSKKRFAGCLDYFRHLLFHYTVARQGMWRLHRVEETDNPTRLAVWEATSHFAFASFL
                     AELICDFVPPVAGDHELYELWLDLEQNLAGHPLGITTTIEYLIRLFELTGYGPNLTHC
                     HHCQKDASETPLYFDERQGQGICPQCDVSPLSVHALQRPLIERVRRGQKQMFSEPNQG
                     RAFLRQLVLFSENIRQKRSPSGVFFLETLL"
     gene            complement(1728..2642)
                     /locus_tag="VJC18_10265"
     CDS             complement(1728..2642)
                     /locus_tag="VJC18_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLD45808.1"
                     /translation="MSITVVGSVALDTIETPCGRSERCLGGSASHFSLSASYFTDVKL
                     VGVVGADFPQKHLDLFRSHHIDLTGLQIIPDGKTFFWAGRYDDVNVAQTLETQLNVFE
                     QFKPALPATYQNADVLFLANIHPDLQSHVIAHAGKPGLIALDTMNLWINTTRESLERA
                     MAQVDVITINDGEVKLLTGENNLPRAVEKLQARGPKTIVVKRGEYGAVLFHGDQCFSA
                     PALPVKVVKDPTGAGDTFAGGMMGYLDRQKTFDFVTLKTAVICGSVMSSFNVQEFSCE
                     RLKNLTTPEIMARFEEFMRLTKFERVQW"
     gene            2915..>3974
                     /locus_tag="VJC18_10270"
     CDS             2915..>3974
                     /locus_tag="VJC18_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HLD45809.1"
                     /translation="MTPTPFCHLHLHTEYSLLNGALRIPQAIALAKQTNMPALAITDY
                     GNIFGAVEFFGEAKAQGIKPILGCELFLPSTDDHKIKKFVRGQDVYWQIVLLVMNQTG
                     YLNLSQLITKSYLEGFYYKPRVDTKLLAQHNEGLIALSSGFHSEINHHLYGDNLQAAK
                     AAAQKYAALFPDRFYIELQDHGLPDHAKTNGTLVKIANELGIPTVATNNIHYLKPDDA
                     EAFEMLRAIQLSPFVASPYDSLQFTTDSYYFKSAEEMQNQFAEYADALSNTKLIAEKC
                     HFSFETGKYHLPRYEPPKDKTLNDYLAEQAREGLHDRWPQIQKVSKATQTDLVKYQER
                     LEHEIKVVQEMGFAGYFLI"
BASE COUNT         1077 a         1060 c          913 g          924 t
ORIGIN      
        1 aggctgtcgt acggctgaat tttataaatg ttgtcgtcca tcacgtacgt tttgaccctc
       61 agatcgatgt tgtctttggc cagttcatga atttgccgga aggccaaacg gggattgagg
      121 tgaaacgtga tcggcacctg agacgccatc agctgatact tgaggatcac agtgttctcg
      181 ttttgcggca tgatgagttc ccgcacgaaa cggatatcac cgatacgata aactgttttg
      241 ggatgcgtgt cgtattcgaa cgactcgatg tacttgtggc ctgtgggatg aaacacccct
      301 ggaaatttgt tggtggacaa attgaacacg tacccaccca cacgaatcgc cccgcccatt
      361 ttggagagca ggacaaattt ggcattgtgc tcgggcaggg tcgccaccag cagaccatga
      421 tacttgcgcg tgtggcaacc cacaatggtg gtggaggcat acccacccaa accgttggtt
      481 tcgagccact cacagcgcag agacgactcc aaattcagac aatcgtttct tgaaaaatga
      541 aaggtcattt gatttcccgt gggttacacg aacacatacc tgagggtagc acaaggctta
      601 aaacagcaaa ccatcagttc gtttacagct ggcagaacaa atcaaagatg ccatggcaat
      661 gcttcgaagc gacaatcgat tagttacgca gtgactctgc tttcttcgag ataagagcca
      721 atttcgtttt gcccatcctg actttcggtc cacttggtga atccctgacg acgcagatcg
      781 tccaaaagat ccaaccactc atcgtggtcc cagcactccc ggcctttgag ccaggcattg
      841 agttcttttc tgggaaaacg ctcaatgacc ttcttctttg tgtttttgtt ttttagcata
      901 attctctcct gaatataaaa gccgctactc actacctcat ctcccgtgaa aatcaacagg
      961 taattggtgc gaaaaagtaa tccgtcacaa aagggtttca aggaaaaaca ccccagaagg
     1021 actgcgtttt tgcctgatgt tttccgaaaa gagaaccaac tgcctcaaaa aggcacgccc
     1081 ctgattcggt tcggaaaaca tctgcttctg accacgacgg accctctcga tcaagggtct
     1141 ttgcaacgcg tgtaccgaca gaggggaaac gtcacattgg ggacaaatgc cttgcccctg
     1201 acgctcatca aagtagaggg gagtttcact ggcatccttt tgacaatgat gacagtgggt
     1261 taaatttggg ccataccctg tcaactcaaa caagcgaatc agatattcaa tggtagtggt
     1321 aatgcccaat gggtgaccag ccaaattctg ctccagatcc aaccagagtt catacaactc
     1381 gtggtcgcca gccacggggg gaacaaaatc gcagatgagt tctgccaaaa aactggcaaa
     1441 cgcaaaatgt gaggtcgcct cccaaacggc taacctcgtt gggttgtctg tttcttcgac
     1501 gcggtggagc cgccacatcc cttgacgcgc aacggtataa tgaaaaagaa gatgtcgaaa
     1561 atagtccagg caaccggcga aacgtttttt ggattttcga gaacctgagg caaagaaagt
     1621 taccacgcca attttttgaa gcaaacaggt gacaatctcg tcggcctcac catagggaat
     1681 cttcctcagc acgatgccac gtgtggcttg ttgatctgtg tctctggtca ccattgaaca
     1741 cgctcaaact ttgtgaggcg cataaactct tcgaaccggg ccatgatctc aggtgtcgtg
     1801 agatttttga gtcgttcaca actgaattcc tggacattga agctcgacat caccgaacca
     1861 cagatcacgg ccgtcttgag ggttacaaaa tcgaatgttt tctggcgatc cagatatccc
     1921 atcatgcccc ccgcgaacgt atcaccagcg ccagtggggt ccttgaccac tttgacgggc
     1981 agtgccggtg cactgaaaca ttggtccccg tgaaagagca cagcgccata ctcgccccgc
     2041 ttcacgacaa tcgttttggg tcccctggcc tgaagttttt cgaccgccct ggggagattg
     2101 ttctcgcctg tgagaagttt gacctcgcca tcgttgatcg tgatcacgtc gacctgcgcc
     2161 atcgcccgtt caagtgattc cctggtggtg ttgatccaaa gattcatcgt atcgagagcg
     2221 atcagaccag gtttgccggc atgcgcaatc acgtgactct gcagatcggg atgaatattg
     2281 gccagaaaaa gaacgtccgc gttttgatac gtcgcaggca aggccggctt aaactgttca
     2341 aatacgttca gttgcgtttc gagagtttgt gccacgttca cgtcatcgta acgccccgcc
     2401 cagaaaaagg tcttgccatc gggaatgatc tgcaaccccg tcaggtcaat gtggtgggac
     2461 cgaaacaggt cgaggtgttt ttgagggaaa tcagccccca cgacacccac cagcttcacg
     2521 tccgtgaaat aactggccga cagggaaaag tgagaggcgg aaccacccaa acagcgctca
     2581 ctcctgccac agggggtttc aatcgtgtcc agcgccacag aaccgacaac agtaatgctc
     2641 atgaaaaata cctcgtgaaa tataatttgt tccagtagaa taaaataaat tggaacttat
     2701 ggcaaatgaa tatcgaatca cgtcccgcaa agcgggatca gtcctatggc tgaaacaagg
     2761 aatttcgaca cctgtcctga acgaagttga aggattcaat attccttgat cgctattcga
     2821 tattcactac cctttcagct aattaaagtt gacacccttg tcattgagcg caaaaagggt
     2881 acagacataa cccataaccc atgacccatt aaccatgacc cctaccccat tctgccatct
     2941 tcacctccat accgaataca gcctcctcaa cggcgctctg cgtatccctc aagccatcgc
     3001 tctggccaaa caaaccaaca tgcccgccct ggctatcacg gattatggca acatttttgg
     3061 tgccgtcgaa ttctttgggg aggccaaggc gcaggggatc aaacccattt tgggttgtga
     3121 acttttttta ccttccaccg acgatcataa aatcaaaaaa tttgtacggg gacaggatgt
     3181 gtactggcag atcgtcctgt tggtcatgaa tcagaccggc tacctgaatc tctcgcaact
     3241 catcaccaaa tcatatctcg aggggtttta ttacaaaccg cgcgtcgaca cgaaactact
     3301 ggcgcaacac aacgaaggcc tcatcgcact ctcatccggg tttcacagcg aaatcaatca
     3361 ccacctctac ggcgacaatc ttcaagcggc caaagcagcg gcgcaaaaat acgcagctct
     3421 ttttcccgac cgcttctaca ttgaactgca agatcacggt ctgccagacc acgccaaaac
     3481 aaatggcacc ctcgtgaaga tcgcaaatga gttgggaata cccacggtgg cgaccaacaa
     3541 catccactac ctcaaaccgg atgacgccga ggccttcgaa atgctccggg ccatccagtt
     3601 atctcctttt gtcgcctcgc catacgacag tctccaattc accacagaca gttattattt
     3661 taaatccgct gaagagatgc aaaatcaatt tgccgagtat gcagacgccc tgagcaacac
     3721 aaagctcatc gcagagaaat gtcacttcag tttcgaaacg ggcaagtacc atttaccacg
     3781 ttatgaacca cccaaagaca agaccctcaa tgattatctg gccgagcagg ccagagaagg
     3841 tctccatgac aggtggccac agattcaaaa agtctccaag gccacacaga ctgacctggt
     3901 caaatatcag gaacgactcg aacacgaaat caaggtcgtg caggagatgg gctttgcggg
     3961 ttactttctg atcg
//
LOCUS       DATDUN010000735         1726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_115359, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000735 DATDUN010000000
VERSION     DATDUN010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115359"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VJC18_10275"
     CDS             complement(<1..363)
                     /locus_tag="VJC18_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45810.1"
                     /translation="MKDQPFDKLRARRPTLQQAQGRRTKDQSRLSLSDCLLAVVFTFC
                     VLSVVFAASLSYAESKGAVTTNPRVQNMSGEDREMFGSLTAKQQKDIRESRIRTGYNA
                     WMVKLALGNPFYNTEHHPV"
     gene            complement(370..>1726)
                     /locus_tag="VJC18_10280"
     CDS             complement(370..>1726)
                     /locus_tag="VJC18_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLD45811.1"
                     /translation="PYGKHFHIITSLPNVFLYKTKPVGQLSTIDLTNENATTFGVGRI
                     EQFSWKNYLDWYSCTECGRCSAQCPAHNSEKPLNPKELSITLRNALYGKTGKLVDKCR
                     GKYNGSLDDAGFIPDETPLLGETISHDVLWSCTSCRACEEACPVCIEYVQEIVDMRRH
                     LVLMEGSFPPELQAVFQNLERNGNPWGISFNDRAAWAADLNIPTLADNPHVEYLYFVG
                     CAGSFDEQNKKVSRAFVKLLQKAGVSFGILGVEEKCNGDSARRLGNEYLAQMLMQENI
                     ANLNKYNVKKIITTCPHCYNTIKNEYSQFGGNFEVITHTDFLKTLITEGRLKPEKAIA
                     QTLTVHDSCYLGRYNDVYDSPRDVLKAIPGIKLVEMTNSRDTARCCGAGGGRMWLEEK
                     LGKRVNQMRLEDVKTTPAELVATACPFCKTMLTDAIGETKTENLNSRDIAELLLESVG
                     E"
BASE COUNT          383 a          419 c          464 g          460 t
ORIGIN      
        1 aacagggtgg tgttcggtgt tgtaaaaggg gtttcccaaa gcgagcttga ccatccacgc
       61 gttgtagccc gtgcggattc tgctttcgcg aatgtctttt tgctgtttgg ccgtgaggct
      121 tccgaacatc tcgcggtctt caccagacat gttttgcaca cggggattgg tggtgacggc
      181 ccccttgctc tctgcataag aaagactggc agcgaaaaca acggacaaaa cacaaaacgt
      241 gaaaacaaca gccagtagac aatcagataa tgataagcga ctttggtctt tggtccgcct
      301 gccctgagct tgttgaaggg ttggtcttct tgccctgagc ttgtcgaagg gttggtcttt
      361 catgatttat cattctccca ctgattccaa caacaactcc gcaatatcgc gagagttcaa
      421 attctctgtt ttggtttcgc caatcgcatc ggtgagcatt gtcttgcaga atgggcaggc
      481 cgtggccaca agctccgccg gcgttgtttt aacgtcttca agtcgcatct ggttgacccg
      541 tttgcccagt ttttcttcga gccacatcct gccgccacca gccccgcaac agcgcgccgt
      601 atctctggag ttggtcattt ccacgagttt gatgcctggg atcgctttga ggacgtcccg
      661 tggtgaatcg tagacatcgt tatagcggcc cagatagcac gaatcatgta ctgtgagggt
      721 ctgggcaatt gccttttcgg gttttagccg gccttcagtg atgagcgtct tcagaaaatc
      781 ggtatgtgtg atgacctcaa agtttcctcc aaactgtgag tactcatttt tgattgtgtt
      841 gtagcagtga ggacaggtcg tgatgatttt tttgacgttg tatttgttga ggttggcgat
      901 gttttcctgc atgagcattt gggccagata ttcattgccc agacgtctgg ccgaatcccc
      961 attacatttt tcttcgaccc ccaaaatccc aaagctgaca cctgccttct gcaggagttt
     1021 aacaaaagct cgcgagactt ttttgttttg ctcatcaaag gagccggcgc aacccacgaa
     1081 atacagatat tcgacatgag ggttgtcagc cagggtgggg atattcagat ctgcggccca
     1141 cgcggcccga tcgttaaaac tgatgcccca cgggttgcca ttgcgctcca gattctgaaa
     1201 aactgcctgc agttctggcg ggaaagatcc ctccatgagg acgaggtggc ggcgcatgtc
     1261 gacgatttcc tgcacgtact cgatgcacac aggacaggct tcttcgcagg cccgacagga
     1321 ggtacacgac cagagcacat cgtgagaaat ggtctctccc agcagggggg tttcgtcggg
     1381 aataaaaccg gcgtcgtcaa gagaaccatt gtatttgccg cggcacttgt ccacaagctt
     1441 gcccgttttg ccatacaacg cgttgcgcaa ggtgatgctg agttctttgg gattcagggg
     1501 tttttcggaa ttatgtgcgg ggcactgggc ggaacagcga ccacattcag tgcaggaata
     1561 ccagtccaga taattcttcc aactgaactg ttcgatccgg ccaacgccaa aggttgtggc
     1621 gttctcattg gtaaggtcaa tggtgctgag ttgcccaacg ggtttggttt tgtagagaaa
     1681 aacattggga agcgatgtga tgatgtgaaa atgtttgccg taagga
//
LOCUS       DATDUN010000736         4074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_115429, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000736 DATDUN010000000
VERSION     DATDUN010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115429"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VJC18_10285"
     CDS             complement(<1..520)
                     /locus_tag="VJC18_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HLD45812.1"
                     /translation="MSEQINKSSSQIDIAHAVKREFSDLPAGQAYDSFPQDSYLPPVN
                     APWHESWYAGPAGDVIKYGLYALAAPVLLVGALGGCNHSADDTDTPDPEATPKDNDDD
                     GFESCDSYAEGTEDHAECVSLLDGDADCNDANATIHPGATETCDGIDQDCDASIDEGL
                     TLYTLYHDGDADS"
     gene            complement(1037..1828)
                     /locus_tag="VJC18_10290"
     CDS             complement(1037..1828)
                     /locus_tag="VJC18_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45813.1"
                     /translation="MLFFKLNEADGKLRRYSILFVSTVIFAFVSYLASFFLFTLQTGL
                     MSVFFTSCLLIPSVEFILEKNKNDVWHKRQTPVLANIDMICSLLVIFAAVILAFIVFV
                     LYLPIETVEQIFFSQIHDTMTHTHLVNTSYQSILSQRIFEFFVFFIISLIFRVGSIFI
                     LVWIASIWGVVYGVYVKQGFQFDLPSIKHYAIVVGLSGFLVFVIRTLSFITACMAGIF
                     LSKSFVKYSFKSKEFQQVLGAVIAILAVSIALLVIAVLGEHQLYQ"
     gene            complement(1871..2410)
                     /locus_tag="VJC18_10295"
     CDS             complement(1871..2410)
                     /locus_tag="VJC18_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45814.1"
                     /translation="MPLSTTRTYIEIPPQRHKGSLLVGFESSTASFLQSQFFLDQQVL
                     LHADGSYTLKHLTLVLERDAVRGLINYLKRWHLDDILNFGSPLYTDALSQGGLEGTFT
                     TDLMGRLFYRIRKTNSRETSLAEIRIILDNNEEPAPRQLEIATEPFLNRFAIDPDVLW
                     QGFRPIGHFLGKRLPKVER"
     gene            3203..4057
                     /locus_tag="VJC18_10300"
     CDS             3203..4057
                     /locus_tag="VJC18_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HLD45815.1"
                     /translation="MRKKEKPDINLFIYSDYRKFLRNYYQLKKKMSPGFSFRSFSKAA
                     GFTSPNYLKLIMDGERNLSGMAIDKFIKALRLNVKEARFFRLLVLMNQASTPEEKDFY
                     ARQIVGSKAYQNIKPLNKAQYCYYSQWYHIPLRELVLRSDFNNDPVWIARQFTPPLTE
                     KQAKEGLETLLKLQLIEMTPDGKFVQTSTSITSGDAVTTEAVKNYHRTMIRLGAEAIE
                     RFPSTQRDISSLTMGMSTQTANEIKKRIADFQEEIMALVEKDSEVQEVVQLNFQLFPL
                     VKGLRLKA"
BASE COUNT         1168 a          881 c         1052 g          973 t
ORIGIN      
        1 aagaatcggc gtcaccatcg tgataaagtg tgtataatgt taaaccttca tcaatgctgg
       61 catcacaatc ttgatcgata ccatcgcagg tttcggtcgc tccggggtgg atggtggcgt
      121 tggcgtcgtt gcaatccgca tcaccgtcca gaagactcac gcactcggca tggtcttctg
      181 tgccttcggc ataggaatca caagattcaa aaccatcatc atcattgtct ttgggagtgg
      241 cttctggatc aggagtatca gtatcatcag cagagtgatt gcaaccaccc aaagcgccaa
      301 ccagcagaac gggggcggcg agggcataca gcccatattt gataacatca ccagcagggc
      361 cggcatacca cgattcatgc caaggtgcat tcacaggtgg tagataggaa tcttgtggga
      421 aggaatcata agcttgcccc gcgggcaaat cagaaaactc tcgtttaaca gcgtgggcaa
      481 tatcgatttg ggaggaactt ttgttgatct gttctgacat ggagtctctc tggggttacg
      541 tacagttgtc tcaccgagtt gcctcatcgt tatcggtttg tctgcggtcc ctgttgttat
      601 gttttttaga cttttttttc aatgaaaaat cagggggttg cgaaagtatt gggaaaaagg
      661 aaaaagaaaa aagaaaaaaa gcaaaaaggt gccacatact ttttgttcca gtgatgcggg
      721 tgttcacgaa gaaggcctag ggtataaggc gtagggcgta aggcggaacc cttcgattcg
      781 tttcactcac tcagggcagg gggcgtaggg cgtaaggcgt aaggcaagaa gtaggggccg
      841 ggcttgaccg gcccgaatca aagtgggtgg cacttttttg tccgattgtt gcttgccccg
      901 tggtgtaact ttacggggac tggcaccttt gattgcgagg aacgtagtga cgaagccatc
      961 cagattgttc cgtcatggag atcgccacgc cctgagcgaa gtcgaagggc tcgcaatgac
     1021 caagaaaaag tctgcgttat tgataaagct gatgttcgcc gagcacggcg atgacgagaa
     1081 gtgcaatgga gacagccaaa atggcaatga cggcccccaa tacctgctgg aattctttcg
     1141 acttgaagga atatttgacg aaacttttgg acagaaaaat gcccgccata caggccgtaa
     1201 tgaacgacag cgtacggatg acaaaaacca gaaaaccaga gagccccacc acaatagcgt
     1261 agtgtttgat gctgggcagg tcgaactgaa acccctgttt gacgtagaca ccgtacacca
     1321 cgccccagat tgatgcgatc caaaccagaa taaaaatgct cccgaccctg aagatgagcg
     1381 agatgatgaa gaatacaaaa aattcgaata tacgctggct caggatggat tggtacgagg
     1441 tgttgactaa atgtgtgtga gtcatggtgt cgtggatttg cgaaaaaaat atctgctcga
     1501 ctgtttcgat agggaggtac agaacaaaaa caataaaagc cagaatcacc gcggcaaaga
     1561 tcaccaggag tgaacagatc atgtcgatgt ttgccaggac aggggtctgg cgtttgtgcc
     1621 agacgtcgtt cttgtttttt tctaaaatga attccaccga agggatgagc agacaactgg
     1681 taaaaaacac agacatgaga cccgtttgca aggtgaagag aaaaaaactg gccagatagg
     1741 acacaaaggc aaagatgacc gtggacacaa aaaggatgct gtaacgcctg agcttgccat
     1801 cggcctcgtt gagcttaaaa aacaacatac ttgattgtag atgaaaacca gggattttga
     1861 aataaaaaaa ttatcgttcc actttcggga gtcttttgcc taaaaaatgc cctattggtc
     1921 taaagccttg ccaaagaaca tcgggatcga tcgcaaaccg attcaaaaat ggttccgtgg
     1981 ctatttcgag ctgtcttggt gcgggttctt cgttgttgtc taaaatgatc ctgatttcgg
     2041 ccaaggaggt ttcacgagaa ttggttttgc gaatgcggta aaaaaggcga cccataaggt
     2101 ctgttgtgaa tgtgccctca agcccgcctt gtgagagcgc gtctgtgtaa aggggtgacc
     2161 caaaattcag gatatcatct aaatgccaac gcttcagata gttaataagt cccctcacgg
     2221 catccctctc aaggaccaag gtcaagtgct ttaacgtgta ggagccgtca gcatgcaata
     2281 aaacttgctg atcaagaaag aattgacttt gcaaaaaaga ggcagtgctg gactcaaaac
     2341 caaccagcag agagccttta tgtcgttgcg gcggtatttc gatgtacgtg cgcgtcgtgg
     2401 aaaggggcat cctgtccttg gtggctctcc tgaaacagag gcgagtcccc caacggggct
     2461 gattatggta ggcgagggtg tcgcgaactg taactgtgat ctgggtcggc gctggcccgg
     2521 acctttgcca aacaggcttt gctgtcaacg cagaatttgt tgacgcgccc ctgacaattc
     2581 gtagtaactc tcgagatgcg cttgtcagtt ctgcaactgg tatgtcgtgt cgaccttgaa
     2641 gtgaaacatc cgggtggcga atgagactca atggtcccag gtcgggcaag aaaggcaggg
     2701 cggggacgct tgctttggtg gggcaatata aggttggaaa agcgaacata ttgtatcccg
     2761 tattttcggg cctgccattt acaggcagag atgccctgac atcttatcga caggacaggc
     2821 tgaaagttgt gcaaattata cgccctcaag tcatgcgaag tcttgtatac tataatgcac
     2881 aatatatata aataatcata atattaataa taaacatcaa taagttaaca gttcatatgt
     2941 atactataag aatacaaaaa atacatttaa tgctctactt tctctttctt ttgtgggatg
     3001 atgggggtag aaagaggcgt gcgtacgtgt tgacgtgcgt acgtgttgac gttggggaag
     3061 ggctgaaagc tgaaagagat tgtcattgcg aggaacccga gctgtagcga ggatgacgtg
     3121 gcaatccacg atttagaaga tggagatcgc cacgactcat agtgtgagtc tcgcgatgac
     3181 gagctacgat ggtcgtgcca ttatgagaaa aaaggaaaaa ccagatatta acctcttcat
     3241 ttattctgat tatcgaaaat tcctgagaaa ctattatcaa ctcaaaaaaa agatgagtcc
     3301 gggtttttct tttagatcgt tttccaaagc cgcggggttt acctccccca attatttaaa
     3361 actcatcatg gacggggaac gcaatttgag tggaatggct atcgataaat tcatcaaagc
     3421 actgaggctc aatgtcaaag aggcccgatt tttcagactg ctcgtgctca tgaatcaagc
     3481 atccaccccc gaagaaaaag atttttacgc gcgccaaatt gtgggcagta aagcctatca
     3541 aaacatcaaa ccgctcaaca aggcgcagta ttgttactac agccagtggt atcacattcc
     3601 tttgcgtgaa ctggtcttgc ggtccgactt taacaacgac ccagtctgga tcgctcgcca
     3661 gttcacgccc ccactcaccg aaaaacaggc aaaggaaggg ctcgagacgt tgctcaaatt
     3721 gcagttgatc gaaatgaccc ccgacggcaa attcgtacag accagcacct ccatcacatc
     3781 aggcgatgct gtgaccacag aggcggtcaa aaattatcac cgcaccatga tccggcttgg
     3841 cgctgaggcg atcgagcgtt ttccgtcaac tcagcgcgat atttcgtcac tcaccatggg
     3901 gatgagcacg cagacagcca atgaaatcaa aaaaagaatt gccgattttc aggaagaaat
     3961 catggcgctg gtggaaaaag acagcgaggt tcaagaagtg gtgcagttga attttcaact
     4021 gtttccgttg gtgaaaggct taaggcttaa ggcttaaggc ttaaggcgta agga
//
LOCUS       DATDUN010000737         1403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_371010, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000737 DATDUN010000000
VERSION     DATDUN010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371010"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1003)
                     /locus_tag="VJC18_10305"
     CDS             complement(200..1003)
                     /locus_tag="VJC18_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HLD45816.1"
                     /translation="MVVLKKTKSRLDQLLVKKDLVESRAKAQALIMARQVLVNGVVAD
                     KAGAVFADDVDVVIKEVMPFVSRGGLKLEKALREFDVNITDKVCLDVGASTGGFTDCL
                     LQNGAKKVFCVDVGYGQLHWKIRNDPRVVNFERENFRHFDISKITDPIEVVVMDVSFI
                     SVKKLIPKIAEILSGLTAPRLHGPAVLVILVKPQFEAGPKNVGKGGVVRDEKVRERVL
                     LDVTEFVLKQGFENIHITQSPITGADGNVEYLLSATWSLRANTVSVAIS"
     gene            1167..1352
                     /locus_tag="VJC18_10310"
     CDS             1167..1352
                     /locus_tag="VJC18_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HLD45817.1"
                     /translation="MKELIKELEKAGFISRGGKGSHRNYKHPRGVHITVSGSLGDDAK
                     FYQERALKEALKAAGNK"
BASE COUNT          402 a          338 c          304 g          359 t
ORIGIN      
        1 ccgtcgagcc ttctttggtt ttgagcatga gataacctcc ccaatttcaa ggttaatgaa
       61 tcaaagattg ggcataaacc gaaaagacaa gttatttcca gtaaaaaata tgtttatacg
      121 tatggcgtca gtgaatggtg cgaagtcatc acgagttggg ctttagcccg acgtggtgat
      181 ctacaagaaa taattatttt caggagattg ccacgctcac tgtgttcgct cgcaatgacc
      241 atgtggcaga caacaaatac tcaacattcc cgtccgcacc cgtgatgggc gactgggtga
      301 tgtgaatgtt ttcgaaaccc tgtttgagta caaactccgt cacgtccaaa agaacccgtt
      361 ctcgaacttt ttcatcgcgc acaacaccac ccttgcctac atttttgggt cccgcctcaa
      421 attggggttt gactaaaata acaagaaccg cagggccgtg gagccttgga gccgtgaggc
      481 ctgaaagaat ttcagcaatc tttggaatca gttttttgac cgaaatgaac gacacatcca
      541 tcaccaccac ttcgatagga tcggtaattt tggaaatgtc aaaatgtctg aagttttcgc
      601 gttcaaaatt caccacgcgg ggatcgtttc tgatcttcca atggagttgt ccgtacccca
      661 cgtccacaca aaacactttt ttggccccat tttgcaaaag gcagtccgta aacccgccgg
      721 ttgaggcccc cacatcgaga caaaccttgt ctgtgatgtt gacatcaaat tcccgcaaag
      781 ccttttccag tttgagcccc ccgcgactaa caaagggcat gacctctttg atgacaacgt
      841 ccacatcgtc tgcaaaaaca gcacccgcct tatcggccac aacaccgttc accaaaacct
      901 gtcgggccat gatgagcgcc tgcgccttgg cgcgggattc cacaagatct tttttcacca
      961 atagctggtc cagacgtgat ttggtttttt ttagcacaac catgacagtt cctttaaggg
     1021 cacctaaaaa ccatctccaa caatttgtaa ttgttagtca agttctggtg atgcccaatg
     1081 tggtacccta tcttacaaaa cttgtcttgt gtcttttgac ctgctattat agtatcaata
     1141 tagtatcatt atacatgccg cgaaaagtga aagaattgat caaagaactc gaaaaagctg
     1201 gctttattag tcgtggtggc aaaggtagtc atcgcaatta caagcatccc aggggcgttc
     1261 acataacggt cagcggaagc ctgggagacg atgctaagtt ctaccaggaa agagccctta
     1321 aagaggcatt gaaagcggca ggcaacaaat gaggcagagc aaaagaggat tcttatgaag
     1381 gaacgaaaca aatatttgaa gat
//
LOCUS       DATDUN010000738         1825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_239263, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000738 DATDUN010000000
VERSION     DATDUN010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239263"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1389)
                     /locus_tag="VJC18_10315"
     CDS             complement(151..1389)
                     /locus_tag="VJC18_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLD45818.1"
                     /translation="MFALKQRNHQAVSYASKLTQFLQVLRNHPVLSENYLDMLSQVTR
                     VLDATCASLYLYEKKTNMFILRKWSGHEPCRCSISGDYEFVSFIKNRGTVVHRSEFSP
                     LTSNEMRNPALYYFQQTLSNVVCPLLDTSEWIGLLNLQVEDVSRHEAIVFYNDLIELF
                     AEHLKQWLICQKLAQDNRRLSELSDIKNQLLSNVTHELQTPLNAILGVSEVLLASPDE
                     ELGSETKLKVGFIEKAGKELSQTVSNLLNLVQIEAKKDTLRREKIAILGVIREVALLY
                     TESCTKKSVQIVIPSTGDDICVFAHADQLRTVFMNLLGNAVKFTQKGHIGISLKKSGE
                     MLHVSVFDTGIGIDDEKLGLIFEEFYQADGSQTRLYGGTGLGLAIAKKIISIHQGRIW
                     AESQKGVGSKFTFTLPLYPV"
     gene            complement(1634..>1825)
                     /locus_tag="VJC18_10320"
     CDS             complement(1634..>1825)
                     /locus_tag="VJC18_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
                     /protein_id="HLD45819.1"
                     /translation="YKPQGMNVGMNLGEAAGAGIRDHLHYHIVPRWNGDHNYMTVVGS
                     TRVLMEDLSTTYKKLKKVF"
BASE COUNT          475 a          460 c          430 g          460 t
ORIGIN      
        1 ctcagggagc ccctccttat caccgcaagg cgggatcccg tttgcgggac aaaaaggagg
       61 ggcggtgccc tcctgttgct tgttgcgcaa taagatttca aagagaaaag ccttttttac
      121 ccacttttat gtcgtacacc ctagagacac tcacacagga tacaacggca aagtgaatgt
      181 gaattttgac cccacaccct tttgcgactc cgcccagatg cgcccctgat gaatggaaat
      241 gatttttttg gcaatcgcca gccccaaacc cgtcccgcca tacaggcgag tttgcgaccc
      301 atcagcctga taaaattctt caaaaataag ccccagtttt tcgtcatcga tcccaatgcc
      361 ggtatcgaac acagacacat gcaacatctc accgcttttt ttaagactga tgccaatgtg
      421 acctttctgc gtgaacttga ccgcgttccc cagaagattc atgaagactg tccggagctg
      481 gtctgcgtgg gcaaaaacac aaatgtcgtc tccggtagaa gggatcacaa tctgcacact
      541 cttcttggta caggactcgg tgtagagcag agccacttcg cgaatcacac ccaaaatggc
      601 aatcttctcc ctgcgcaagg tatccttttt ggcctcgatc tgcacgaggt tcaacaaatt
      661 gctcactgtc tgactgagtt ccttgcccgc tttttcgatg aaacccacct tgagtttggt
      721 ctctgaccca agctcctcgt cgggagaagc aagcaacacc tcagacacac ccagaatcgc
      781 attgagaggt gtttgcagtt catgggtgac gttggataag agttgattct tgatgtcgct
      841 cagttcggac aggcgacgat tgtcctgcgc gagtttctgg caaatgagcc attgtttgag
      901 gtgttcggca aacaactcaa tcagatcatt ataaaacacg atggcctcat gacgggaaac
      961 atcctctacc tgcaggttca agagcccgat ccactcgctc gtgtccagaa ggggacagac
     1021 cacgttggaa agtgtttgtt gaaaataata gagggccggg ttcctcatct cgttggacgt
     1081 gaggggggaa aactcactgc ggtgcaccac cgttcccctg tttttaatga aggaaacaaa
     1141 ttcataatcg ccgctgatcg aacaacgaca aggctcatgt cctgaccact ttctcaaaat
     1201 aaacatgttc gttttttttt cgtacaaata gagggaggcg caggtggcat cgagcacgcg
     1261 ggtcacctgg gacagcatgt ccagataatt ctcgctgagc accgggtgat tcctgagcac
     1321 ctggagaaac tgcgtcaact ttgaggcgta agacacagct tgatgatttc tttgtttgag
     1381 ggcgaacatg aatcaattta tctgatggga acagtgtatc ttgttgtcaa tgattgtgca
     1441 atcgataaga cacaagccaa aacgtgttca agtgttcacg tgtttatgtg ttcacgaaaa
     1501 ggccgacagc caacggtcta cagctaacag ccgaagaaga acgtttggct attgacagtc
     1561 ggcagttggc tgttgcccgt tagtcataca cacaaaaaat ttaaaaaatc gattgtaaca
     1621 tgctggtaaa atgctaaaat acttttttta atttcttata ggttgtggaa aggtcttcca
     1681 tcaacacacg agtgctcccg acgacggtca tgtagttgtg atcgccgttc cagcgcggca
     1741 cgatgtgata atgcagatga tcgcgaatcc ccgcgccagc ggcctcgccc agattcatcc
     1801 ccacattcat gccctgtggt ttgta
//
LOCUS       DATDUN010000739         3451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_405374, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000739 DATDUN010000000
VERSION     DATDUN010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405374"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VJC18_10325"
     CDS             <1..645
                     /locus_tag="VJC18_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HLD45820.1"
                     /translation="GVVSPSAVLQKYYYPLEEARADLFSLYFMLDPKLIELGLLPSLD
                     AGKAAYQNTVTKGLLKQLARIELGQDISDSHMRDRHLIASWAYEKGQTDNVIEKIIKE
                     GKTYYVINDYQKLRGLFAQLLGEVQRIISEADYEAGKALVERYGIKINKDLHREVLDR
                     WAKLGVAKYSLFLMPYMKPVLEGDKLVDVILEYPTDFVEQMMLFGSQYSFLPAM"
     gene            complement(666..1196)
                     /locus_tag="VJC18_10330"
     CDS             complement(666..1196)
                     /locus_tag="VJC18_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45821.1"
                     /translation="MKKKTTTHQYGNSKGTSLIEVILAMVISLIIILVIVQTLIAHLD
                     AFYYVANRRSSVADARYALIVISNNLKNISPGTITDIQSSQFTYTESMGPTTFRFYTA
                     DSISRNNDVLLKDVNNFWFNYFDQNGAPLSPTVANIPNVRRIRVSIETKPHNDEGSIT
                     ISAFVVPRDFLGYDIQ"
     gene            complement(1237..1656)
                     /locus_tag="VJC18_10335"
     CDS             complement(1237..1656)
                     /locus_tag="VJC18_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45822.1"
                     /translation="MLSTKNKKGISLIEAVLTVFVLAIGFMSSMQAIQQIVSSSNFSD
                     REIVAAQLAAGKLEGILADNKFRGFGYIIPANYPDETLTGTYAGYRRKTNIFQVAPGF
                     LNDVAAPGQVLKHIDVKVYYGPNEVDSVTITELVGIR"
     gene            complement(1662..2435)
                     /locus_tag="VJC18_10340"
     CDS             complement(1662..2435)
                     /locus_tag="VJC18_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45823.1"
                     /translation="MPEWLKNTVLKRPLGSVMLPAIITMIVLSYATLSLVQIQAAGLA
                     EAANHAEKLKSEHLFLAGIEFSYALLNNGQSPATNGDRTLGSGNINVTANPSTENVTV
                     KGEVPKAKKQGTANMSFSKSCIVIDDNYVFLFGNAFGDIQNIHIRKTCHNKFIINKMY
                     VTWTNSNLAQKVTKIDLDSAIYYDAFHDPPTGSPVGGANSGVLIDINDITITDANEHT
                     FGYVRLQYPENGAPPPGTVFTIRLDFQDGSSKTKDLVYN"
     gene            complement(2476..2898)
                     /locus_tag="VJC18_10345"
     CDS             complement(2476..2898)
                     /locus_tag="VJC18_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45824.1"
                     /translation="MKIKDFFYADKIPFRIILFVTLVLCFYNLYHMRHAAVLVRISFL
                     LTTVCAIALSLCAAIPWYGLQHKGLGIELHFYRLWVPTIYSFLVLNILFAFGIASVTL
                     NFCLLIVYAAFLATNIVLLKLHFKDKDKTPPCHWAKHR"
     gene            complement(2888..>3451)
                     /locus_tag="VJC18_10350"
     CDS             complement(2888..>3451)
                     /locus_tag="VJC18_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD45825.1"
                     /translation="SVIALLTLSGHKTVSLAPEAGTDRLRFSLNKKITNSVLLEKIRL
                     LADAGMHRIKLYFMIGLPHETDEDVAGLIDLVRGAHALLTTSAKKSRHMTRLILSINP
                     FVPKPRTPFAQQAFAETTQLHDTMNHIRKSLRPLGNLVLHHESIFLSLVQAWLANGTS
                     EVNQLILSTFESQGNLKKALKKISYED"
BASE COUNT          837 a          741 c          962 g          911 t
ORIGIN      
        1 ggtgtggtga gtccgagtgc tgtcttgcag aagtactatt atcctttgga ggaggcgcgg
       61 gccgatttgt tcagcctgta tttcatgtta gatcccaagt tgattgaatt gggcctcttg
      121 ccctctcttg atgcgggcaa agccgcgtat caaaacaccg tcacgaaagg actcttgaaa
      181 cagctggcgc gcattgagtt ggggcaggac atttctgact cgcacatgcg cgaccgccat
      241 ctcattgcca gttgggcata tgagaagggt caaacggata acgtgatcga aaaaatcatc
      301 aaggaaggca aaacttatta tgtgatcaac gattatcaaa aactcagggg gttgttcgcc
      361 caactactgg gagaggttca gcgcattatt tctgaggctg attatgaggc gggcaaggcg
      421 ttggtggagc ggtacggcat caagatcaac aaggacttgc acagagaagt attggatcgt
      481 tgggccaagc tgggtgtggc caagtattcg ctgtttctca tgccgtacat gaagcctgtg
      541 ttagagggcg acaagcttgt tgatgtgatt ctggaatacc ccacggattt tgtggagcag
      601 atgatgttgt ttggaagcca gtattccttt ttgccggcaa tgtagcaaca cggcgagctg
      661 tgttgctact gaatatcata ccccaaaaaa tctcggggca cgacgaaggc cgatatggtg
      721 atggaaccct cgtcgttgtg gggtttggtt tcgatcgaga ccctgattct cctcacgttg
      781 gggatgttgg ccactgtcgg tgagagaggg gcgccattct ggtcgaaata attaaaccag
      841 aagttattca catctttcaa caagacatca ttgttgcgcg agattgaatc ggccgtataa
      901 aaacggaacg tggtggggcc catggattcg gtataggtga attgtgatga ctggatgtcg
      961 gtgatggtcc cagggctgat gttttttaag ttgttgctga tcacgatgag ggcataccga
     1021 gcatcggcga cggaagatct tcggttggct acgtagtaga acgcatcgag gtgcgcgatg
     1081 agggtttgga cgatcacgag gatgatgatc agtgagatga ccatggccaa aataacctcg
     1141 atgagtgagg ttcctttgga gttgccgtat tgatgtgttg ttgtcttctt cttcatgttt
     1201 accacaaaac agcagttaca ccgttatctt attgattcat cttatcccga cgagttccgt
     1261 tatggtgact gaatcaactt cattcgggcc atagtaaacc ttgacgtcaa tgtgcttaag
     1321 cacttgcccc ggggccgcga cgtcgttgag aaacccggga gccacctgaa agatattggt
     1381 ttttcgccga taacccgcat aggtccctgt cagagtttcg tcgggataat tcgcaggaat
     1441 gatgtagcca aaacctctga acttgttgtc ggccagaatg ccttcgagct ttcccgccgc
     1501 cagttgcgcg gcaacgatct ccctatcaga aaaattggag ctgctcacga tctgctggat
     1561 ggcctgcatg ctactcatga agccaatggc cagcacgaac actgtgagaa cggcttcgat
     1621 gagtgagatc ccttttttat tttttgtcga tagcatggtt gttagttata aaccaaatct
     1681 tttgtctttg aactgccgtc ttgaaaatca aggcggattg taaaaacagt ccctggtggc
     1741 ggagccccgt tttcaggata ttgcaagcgg acatagccaa acgtgtgttc gttggcatcg
     1801 gtgatagtaa tgtcgttgat gtcgatgagg actccgctat tggcgcctcc gaccggggac
     1861 cctgtgggtg ggtcatgaaa ggcgtcataa taaattgcag aatccaggtc gatcttggtg
     1921 accttttggg cgagattact atttgtccag gtaacgtaca ttttgtttat gatgaatttg
     1981 ttgtgacagg tttttcgaat gtgaatgttt tgaatatcgc cgaacgcgtt gccaaaaaga
     2041 aaaacgtaat tgtcatcaat gacgatgcaa ctcttgctga aggacatgtt cgctgtcccc
     2101 tgttttttgg ccttagggac ctctcccttg acagtgacgt tttctgtgct ggggttggcc
     2161 gtgacgttga tgttgcccga tcccaaggtt cggtcgccgt tcgtggcggg tgattgaccg
     2221 ttattgagca gggcgtagga aaattcaatg ccagccaaaa acaggtgttc ggacttgagc
     2281 ttttcggcgt ggtttgccgc ttcggccagg cccgcagctt ggatttgcac gagcgacaac
     2341 gtggcatagc tcagcacaat catcgtgatg atggcgggca acatcaccga gccgagaggc
     2401 cttttgagga ctgtgttttt aagccattca ggcatatgaa accattaccc tatttccttt
     2461 gtaaaaggca actcctcatc tgtgtttggc ccagtgacac gggggggtct tgtccttatc
     2521 cttaaaatgc aatttgagca aaacgatatt ggtagcgaga aacgcggcat acacaatcag
     2581 caagcaaaaa ttcaaggtca cagaggcgat gccaaaagca aataatatgt tcaaaacgag
     2641 aaaggaataa atggtgggga cccagagtcg atagaagtgc agctcgatcc cgagcccttt
     2701 gtgttgcaat ccataccagg ggatggcggc gcagagcgag agggcgatcg cacaaactgt
     2761 ggtgaggagg aatgatatcc tcaccaaaac ggcggcgtgc ctcatgtggt agaggttata
     2821 aaaacacagg accagtgtga caaagagtat gatgcgaaaa ggaattttgt cggcgtaaaa
     2881 aaaatcttta atcttcataa gaaatttttt tgagggcctt cttcaggttg ccttgtgact
     2941 cgaaggtgct cagaatcagt tggttgacct cactcgtccc attggcgagc caggcttgca
     3001 cgagactcag aaaaatactt tcgtggtgca agaccagatt gcccaagggg cgcagggatt
     3061 tcctaatatg attcattgta tcatgcaact gggttgtttc tgcgaaggcc tgttgcgcga
     3121 aaggggttct tggtttgggc acaaacgggt tgatgctcaa gatcaagcgg gtcatgtgtc
     3181 tgctcttttt agcgctggtg gtgagcaggg catgtgctcc gcgcaccagg tcgatcagtc
     3241 ccgcaacgtc ttcgtctgtt tcgtgaggca gtcctatcat gaagtagagt ttgatgcggt
     3301 gcatgcccgc gtcggccagc aaacgaattt tctccagcag aacagaattg gtgatttttt
     3361 tgttgagaga aaagcgcaac cgatctgtgc ccgcttcggg tgccaacgag acggttttgt
     3421 gcccgctgag ggtgagcaag gcaatcaccg a
//
LOCUS       DATDUN010000740         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_239307, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000740 DATDUN010000000
VERSION     DATDUN010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239307"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..823
                     /locus_tag="VJC18_10355"
     CDS             212..823
                     /locus_tag="VJC18_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45826.1"
                     /translation="MKWHDCDNKLQKSGFKVFSNREFQGISGASAVASKFMLMRYTNK
                     GLLCRLKRDLYQCTNRMASPWLIANQLYKPSYLSLESALSYYGLIPESVYSVTSVTTK
                     TTRGFEAGNHLYSYRTIKASAFGGYRSVMIADEQIMMAEREKALADYLYFVFLKKIVL
                     NERLSFTDVKKRRLHDYLLLFERPDLEEWYRHDFKISDHRIAR"
     gene            786..1511
                     /locus_tag="VJC18_10360"
     CDS             786..1511
                     /locus_tag="VJC18_10360"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLD45827.1"
                     /translation="MISKYQIIELRDEWKTTELNVAREYVQHVFLSSLFRGLRKQVKL
                     AFKGGTALRIIWGSPRFSEDLDFTAWGTEYHIGECLREAVNEASKAGLDIKLVASNKT
                     SGGWFALAKTQLHDWPVSIEWNISLRQVRQTTVVPVLVTSPLWASYSVLALNAEQMVF
                     EKIEALFRRKKPRDFFDVYFMLRNRLGISEILRQKENLLAEVKKLNAGIVSRELRLFT
                     PQNQWPIMKQLPRLLKEELGRLG"
     gene            complement(1562..2158)
                     /gene="recR"
                     /locus_tag="VJC18_10365"
     CDS             complement(1562..2158)
                     /gene="recR"
                     /locus_tag="VJC18_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HLD45828.1"
                     /translation="MNNPIDKLVHELGKLPGIGERTAMRLALHILREPSEYGLSLAKA
                     LQETVGKVHFCARCCNIATEELCPICQKHDREDHVVCVVEDIADLQAVEKTYCFRGRY
                     HVLHGALSPIDGIGPEELKIAQLEKRLREDPAISELIIATNPNVTGDATALYLARLLA
                     PYRKKITRLASGIPVGSHIEFIDRSTLSRAFESRREYG"
BASE COUNT          569 a          530 c          565 g          587 t
ORIGIN      
        1 ctcagggagc ccctccttat caccgcaagg cgggatcccg tttgcgggac aaaaaggagg
       61 ggcggtgccc tcctgttgct tgttgcgcaa taagatttca aagagaaaag ccttttttac
      121 ccacttttat gtcgtacacc cgggagggga aaacatcttg acaaaacctc ggattatact
      181 attctagtaa agtagcaaat atgctactta tatgaaatgg catgattgcg acaacaaact
      241 tcaaaagtct ggttttaagg tcttctctaa ccgtgagttt cagggcattt ccggggcaag
      301 cgctgtggcg tccaaattca tgctcatgcg ttacacaaac aagggccttc tgtgcaggtt
      361 gaagcgcgac ctctatcagt gtacgaacag gatggcgtca ccctggttga ttgccaacca
      421 gctctacaaa ccctcttatc tctctttgga gtctgctttg tcttattatg gtttgatccc
      481 cgaatctgtt tattctgtga cttctgtcac cacaaaaacc acgcgcggat ttgaggcagg
      541 gaatcacctc tattcctatc gaaccatcaa agcgtctgct tttggagggt atcgttcagt
      601 catgatcgct gatgaacaga tcatgatggc tgaacgcgaa aaggccctcg cggattatct
      661 ctattttgtt tttttaaaaa aaattgtttt gaacgaaaga ctttctttta cagacgtaaa
      721 aaagcgacga ttgcatgatt atctcctttt gttcgaaaga cctgatttag aggagtggta
      781 ccgtcatgat ttcaaaatat cagatcatag aattgcgcga tgagtggaaa accacagagt
      841 tgaacgtcgc tcgcgaatac gttcaacacg ttttcctttc gagtctgttt cggggacttc
      901 gaaagcaggt gaaacttgct ttcaagggag ggactgcctt gcggatcatt tgggggagtc
      961 cccggttttc ggaagacctc gattttacag cttggggaac ggagtatcac atcggtgaat
     1021 gcctcagaga agctgtcaac gaggcgtcaa aggcaggtct ggacatcaaa ctcgtagcat
     1081 caaacaaaac cagcgggggg tggttcgcgc tggccaaaac acaacttcac gattggccgg
     1141 tcagtattga gtggaacatt tcattgcgtc aggtacggca gactaccgtc gttcctgttc
     1201 ttgtgacttc tccgctctgg gcctcctatt ctgtgctggc cttgaacgca gaacaaatgg
     1261 tttttgaaaa aattgaggcc ttgtttcgga gaaaaaaacc acgtgatttt tttgatgtct
     1321 atttcatgct cagaaaccgc ttgggaatct ccgagatatt gcgacaaaag gaaaatttgt
     1381 tggccgaagt gaaaaaactg aacgctggca ttgtgagtcg ggagttgcgg ctatttacac
     1441 ctcagaacca gtggcccatc atgaaacagt tgccgcgatt gttgaaagag gaattgggaa
     1501 gattggggta aggcgtggtt tcggacttca tcgtgctaca aattgacccg caataaataa
     1561 attaaccata ctcgcgacgg ctttcaaagg cgcgagagag agtactgcga tcgatgaatt
     1621 cgatgtgaga acccacagga attcccgagg ccaaacgcgt gatttttttt cggtaggggg
     1681 ccaaaagtct cgccagatac agtgccgtcg catcgccagt cacattggga ttggtggcaa
     1741 tgatgagttc gctgatggcg gggtcttcac gcagacgttt ttccagttga gcaattttca
     1801 gttcttcggg gccaatgcca tcgatggggg acagcgcccc gtgcaagacg tgataccgcc
     1861 ccctgaaaca atacgttttc tccacggcct gcagatcggc aatgtcctcc accacacaga
     1921 ccacatggtc ctcacggtcg tgtttttgac agatcggaca aagctcttcg gtggcaatgt
     1981 tgcaacaccg ggcacaaaaa tgaaccttac ccacggtttc ctgcagggcc ttggccagac
     2041 tcagtccgta ctcggacggc tcacgcagaa tatgcaaggc caaccgcatg gcggtgcgct
     2101 caccgatacc cggcagtttg cccaactcgt gcaccagttt gtcgatagga ttattcatct
     2161 cgttgatcgt ggatcgcttg tgccccagag ggcacggata gtgtgtcgtc gatcgtaaaa
     2221 tgatcttctc gattcacgat ccacgattat c
//
LOCUS       DATDUN010000741         1576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_79198, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000741 DATDUN010000000
VERSION     DATDUN010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79198"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VJC18_10370"
     CDS             complement(<1..679)
                     /locus_tag="VJC18_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45829.1"
                     /translation="MHSDSGEITTFSIDATMMGGRIPKIGVINNGGICSYNIDGSRTR
                     YLIAYSEMVLLAVYFQHFFDQDLISMETALQSQDVQAQAQQDGSWIDKVDSWTKRFEC
                     SNKDIAFIEASFSAREEVKQVLVKHGIPARKAQEGEQETDVAGHLSDLVCRAGTPFIE
                     NLEQESTSSFGRKKEIESINQLLSAYTCVRQETKTGQTSTKQPDDISSFVAPETLSLL
                     EKLMQFPL"
     gene            complement(713..1003)
                     /locus_tag="VJC18_10375"
     CDS             complement(713..1003)
                     /locus_tag="VJC18_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45830.1"
                     /translation="MSVALPQTSDLESWDAFQHYIDQEPELGQCVEVTDLVATGQADS
                     NERLPSIDEIKSILTRANITIDTAKTLRHQVSRFAAKANMNPDVQASLERES"
     gene            1183..>1576
                     /locus_tag="VJC18_10380"
     CDS             1183..>1576
                     /locus_tag="VJC18_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLD45831.1"
                     /translation="MLIITNARILCPDRIIEKGTLCVDNSIIIEVSDQPQSKKHDPHA
                     EIIDAQGGLVMPGLTNTHMHLYSTFARGMSLVGEAPANFVQILERLWWRLDKALTVED
                     VYLSAVIPLIECVRAGTTTIIDHHASPNF"
BASE COUNT          396 a          356 c          372 g          452 t
ORIGIN      
        1 acaggggaaa ttgcatgagc ttctccagca gtgacaaggt ttctggagct acaaaagagg
       61 aaatgtcgtc aggttgtttt gtgcttgtct gtccagtttt tgtttcttgc ctgacacacg
      121 tgtatgccga caataactga ttgatggatt cgatctcttt ttttctacca aaggatgagg
      181 ttgattcctg ctctaaattt tcaatgaacg gcgtgcccgc ccggcagaca aggtctgaca
      241 aatgtccggc cacatctgtt tcttgttctc cctcttgtgc cttccgggca ggaatgccat
      301 gtttcaccag aacctgtttc acttcttctc tggctgaaaa ggatgcctcg ataaaggcaa
      361 tatctttgtt actacattca aatctcttcg tccaggagtc gactttatcg atccaggaac
      421 catcttgctg tgcttgcgct tgaacatctt gcgactgcaa ggcagtctcc atggaaatta
      481 aatcctgatc aaaaaaatgc tggaaataga cagccaacaa caccatctct gagtaagcta
      541 ttaagtatcg agtgcggcta ccatcaatat tgtaggagca gatgcctcca ttgtttatca
      601 cgccaatttt tggtattcgc ccccccatca ttgtggcatc aattgaaaaa gtggtgattt
      661 ctcctgagtc agaatgcatg tcgtcaaaat ttaaagacag aaatgatggt tgtcatgatt
      721 ctcgttccaa cgatgcctgt acgtcaggat tcatgttggc ttttgctgca aatcgactga
      781 cctgatgcct caacgttttg gctgtatcga tagtgatatt ggctctggtt aatattgact
      841 tgatttcatc gatcgaggga aggcgttcat ttgaatcagc ttgtccggtg gcaaccagat
      901 cagtcacttc tacgcactga cccaattcag gttcttgatc gatgtagtgc tgaaaggcat
      961 cccaagactc caaatcactg gtttgtggta aggcgactga cataaagact cccgtcaaag
     1021 ttaatcgatc atggcattgt gcccaacatc tttgattgta gcatgatcct gagcgatggg
     1081 aaagtgataa attttgagtg atattttctc ttggcgggag tttgttgatc gtttatggag
     1141 tggccagatc attacgtgat acgaaaacgc agagagggat ctatgctcat cattaccaat
     1201 gccagaatat tgtgtccgga tcgaatcatc gaaaagggca ctttgtgtgt tgataacagt
     1261 atcatcatcg aagtgagcga tcagccgcaa tccaaaaaac atgatccgca cgcagagatc
     1321 atcgatgcgc agggcgggct ggtcatgccg ggcttgacca acacacacat gcatttgtat
     1381 tccacctttg cccgggggat gtcgcttgtg ggcgaagccc cggcgaattt tgtgcaaatt
     1441 ctggaaagac tttggtggcg gctggataag gccctcaccg tggaagatgt ttacctgagt
     1501 gccgtgatcc cgctcatcga atgtgtcagg gctggaacca ccacgattat tgatcatcac
     1561 gcctcgccaa atttcg
//
LOCUS       DATDUN010000742         1627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_447163, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000742 DATDUN010000000
VERSION     DATDUN010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447163"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="VJC18_10385"
     CDS             <1..1417
                     /locus_tag="VJC18_10385"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HLD45832.1"
                     /translation="KVYIDEVEDDHGHVVLSKERADAFEAWRQVEEVHEKDGIIDGLV
                     VNKVKGGLSVNLGGIKAFLPGSQIDLKPVKSLDKLIGHKYQFKILKLNKSKGNIVLSR
                     RAILEVERETMRRDILENIKEGQIIRGVVKNITDLTWGRVSHPKEVLKVNEEIDVVVL
                     KYDPKTEKVSLGLKQLQEDPWTKVGNYEPGQRIKGRVVSITDYGVFVEIEAGIEGLVH
                     VSELTWSKKVKHPSKVVKVDDEVEAVILDVDIDNKKIALGLKQLEPNPWDILHQKYPL
                     GSKVTGKIRNITDFGMFVGIENEDIDALIHVSDISWDKNLKWPSDDYKKGQDLEALVL
                     NIDKDNHKFALGLKQLSDDPYAVLMRAYPLGAEVTGIVESVSEKGIVLRLNEQTTALL
                     PSAETGVGRVELKDKFKEGQEVVAQVKKYEDRDRKMIVSVKMLTRRQEKENMSEFLNK
                     QGDASVTFKDIMEKSQKVTNS"
BASE COUNT          455 a          381 c          436 g          355 t
ORIGIN      
        1 caaggtttat atcgatgaag tcgaggatga ccatgggcac gtggtgttgt ccaaggaaag
       61 ggccgatgcc ttcgaagcct ggcgccaggt cgaagaggtg cacgaaaaag acggcatcat
      121 cgatggcttg gtggtcaaca aagtcaaggg cggcctgtcc gttaatctgg gtggcatcaa
      181 ggcctttttg ccgggcagtc agatcgatct caaacccgtc aagagtctcg ataaactcat
      241 tggtcacaaa taccagttca agattctcaa actcaacaag agcaagggca atatcgtgtt
      301 gtcgcgccgc gccattctgg aagtcgaacg cgaaaccatg cgccgcgata tcttagagaa
      361 catcaaggaa ggtcagatca ttcgtggcgt ggtcaagaac atcaccgatc tcacttgggg
      421 tcgtgtcagt catcccaagg aagtcctcaa ggttaacgag gagatcgacg tggtggtgct
      481 caagtacgat cccaagacag aaaaagtttc gctgggcctc aaacaattgc aggaagatcc
      541 ctggacgaag gtggggaatt acgaacctgg tcagagaatc aagggtcgtg tggtttccat
      601 caccgattat ggtgtgtttg tcgaaatcga agcagggatc gaagggttgg tgcatgtttc
      661 tgaactcacc tggtccaaaa aggtcaaaca ccccagcaag gttgtgaagg tggacgacga
      721 agtcgaggcc gttattctcg atgtcgatat cgacaacaaa aaaatcgcat tgggtctcaa
      781 acagttggaa cccaatccct gggatattct gcatcagaag tacccgctgg gttccaaggt
      841 cacgggcaag atccgcaaca tcaccgactt tggcatgttt gtagggatcg aaaacgaaga
      901 catcgatgcc ctgatccatg tgtccgacat ctcgtgggac aaaaatctca aatggccctc
      961 cgatgactac aaaaaaggtc aagacctcga agccctggtg ctcaacatcg acaaagacaa
     1021 tcacaaattt gccctgggcc tcaaacagtt atccgatgac ccctatgccg tcttgatgcg
     1081 cgcgtaccca ctgggtgccg aggtgactgg aattgtcgag agtgtgtccg aaaaaggtat
     1141 tgtcttgcgc ttgaatgagc agaccacagc cttgttgccg agtgccgaaa caggtgttgg
     1201 ccgcgtcgaa ctcaaagaca aattcaagga aggccaagag gtcgtggctc aggtcaaaaa
     1261 atacgaagac cgtgaccgca agatgatcgt ttcggtcaaa atgctgactc gccgacaaga
     1321 aaaagaaaac atgagcgagt ttctcaacaa gcaaggcgat gcctctgtga cgttcaaaga
     1381 catcatggaa aaaagccaaa aggttaccaa ttcgtaaata ttggtgactc accatttcag
     1441 aaaagatacc cagcagtagg ggccggtcgt gaccggtccc tatgcaaagt cagggttggt
     1501 tttaattagc cgcatgaaaa aacatcccat attagcgttt gtcttgttgt tcgttgcgat
     1561 catcgcgatc tcggttgtcg tgatgggtgg actgggcatg atgctctcct ccggttacga
     1621 gacgccg
//
LOCUS       DATDUN010000743         4531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_371269, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000743 DATDUN010000000
VERSION     DATDUN010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371269"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VJC18_10390"
     CDS             <1..421
                     /locus_tag="VJC18_10390"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HLD45833.1"
                     /translation="FLCGATNVQVTTAIMRYGYRIVEDMIEGLSDWMIDHKITSLNEI
                     IGNAAKKLIDPSELNNRYQVVSQIDADKCIGCGQCWISCHDGANQAIKFDASLRKASV
                     DETRCVGCLLCKHICPVDGCITHKEVDTATIKHAAVF"
     gene            835..1965
                     /locus_tag="VJC18_10395"
     CDS             835..1965
                     /locus_tag="VJC18_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HLD45834.1"
                     /translation="MPGFNLVDDLGFLLADYYSIDDAGQSFLDEVHTAHSLLEEVRII
                     LENDQTGLMDQTFDSYEDFYNAWDQFVSRQEQGSIFRSARHTEEDTGMAGALDEAGDA
                     EVMFPPWFAKVKKFFTDVEAKRFQNEDTIYQVRDTGFAQSRLIYIDVYDEAQSIFDVL
                     FTVDQGINELLGVYAEMAPATSLQTLPEGYEPADRKEVIMLCDRLKLDYQVVEEMWAD
                     IVAEGITTADEVLDELKARAVAFAQDRVVSFACKLVSLMKGKKYTPGGKSPRQGFDCS
                     GLVWYVYKSLGLDYTFLGANSFSQSARDPTGAFAKVTDDVRLGDVVWFPGHVGVVTSV
                     ANGRVQFVHSCSRGVVTDDLEEYIKYSPEGGKHFFYRNRALF"
     gene            2186..2380
                     /locus_tag="VJC18_10400"
     CDS             2186..2380
                     /locus_tag="VJC18_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019028241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLD45835.1"
                     /translation="MEKGTVKFFNMDKGFGFIKPDNGAKDLFVHTSVVENGPIAEGDK
                     VEFEVGQGKKGPCAEKVRRI"
     gene            complement(2552..3106)
                     /locus_tag="VJC18_10405"
     CDS             complement(2552..3106)
                     /locus_tag="VJC18_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HLD45836.1"
                     /translation="MKYDIVFAGVGGQGILSMTNMLIQAACDVGLQVKQTEIHGMAQR
                     GGAVTSQVRIADSPIHSGIIAKGTAHYLVATEPMEALRQLDFLSEDGCVISSTDPVKN
                     IAYPELIDIHNAIRKRRHYNLIDMQQILIKINNPKAANIVLLGALVNELQIAGLKDKI
                     RRSIEGLFAHKGDAVVQANLLALS"
     gene            complement(3172..>4531)
                     /locus_tag="VJC18_10410"
     CDS             complement(3172..>4531)
                     /locus_tag="VJC18_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLD45837.1"
                     /translation="VSMKHVGLNVAADPFMNASLTGTQGGLVICVADDPSMHSSQNEQ
                     DSRFYSWFSKILCLEPIDAQEAYDMTLLAFTLSEKWHLPIMIRLTTRVAHSRSNATLS
                     EINHPQKRITLPASFKNWMLLPSNARPQFELLWSKQNDLTQFSNHFEKNVLNIRPNTQ
                     KAVITGGLAHQYFMECGGADDYSYLRVCSYPLPKQKILALLQNADELFVIEEGYPLIE
                     TLIYECLHEIPHRIIVHGRHDKTVPQTGELNTDLVKKSLGYPTPTTTPATNDKLVRPR
                     PPQLCKGCPHTDTFKALNEALRGHEHERVVFSDIGCYSLGYYPPHEAIHSGVCMGASI
                     SMAKAAGENGLKYAIAVIGDSTFEHSGITPLLEGISRQVNFTVIILDNSLVAMTGGQE
                     RIMGAEQFVSFILGMGIDRDHLKIIHPIPKNHAENTRIIREECEYQGPSVILARRECL
                     QK"
BASE COUNT         1134 a          950 c         1184 g         1263 t
ORIGIN      
        1 ctttctttgt ggggcgacca atgtgcaagt cacaacggcc atcatgcgtt atgggtatcg
       61 cattgtggag gacatgattg aggggctgag tgattggatg attgatcaca aaatcacgtc
      121 actcaacgaa atcattggca atgccgctaa aaaactcatc gatccatcag aactcaataa
      181 tcgctatcag gtagtgtcgc aaattgatgc cgataaatgc atcgggtgtg ggcagtgctg
      241 gatttcgtgt catgacgggg cgaatcaggc gatcaaattt gacgcgtctt tgcgcaaggc
      301 cagtgtcgat gaaacccgtt gtgtgggttg tcttttgtgc aaacacatct gcccggtgga
      361 tggttgcatc acacacaaag aggtggatac tgccaccatc aaacacgccg cggtgttttg
      421 agtttttatt tagtctgtgt gcgaaacgaa tcaaaacttg ctccgtcccc acaagacagc
      481 ggtttgtatt tctcaacatt ctcttgaaaa atcctatcta atctctggat cgtcagggtg
      541 tctttatcac tggaatttaa aaatcgatca gagacatttg ataattcttg aggatcagtt
      601 tgaagatcat ataattctgt ttcgccaata tagggagata tgtatttaat cagtttccaa
      661 tttacacttc tccgtcatgg gaggcaggcg cagaatacga ggcaaatttt tccctccaat
      721 ttgataattt gctgtcgata tgccccataa ttgatctcga tagtccaaaa caaacaataa
      781 tatcaaacta ttacataatg ttctcttggc aaggaaattg cagttcagat cggtatgcct
      841 ggttttaatc ttgtcgatga tctgggtttt ttgctggcgg actactacag tattgacgat
      901 gccggtcaat cctttctgga tgaagtccat accgctcatt ccctgttgga agaagtgagg
      961 atcatattag aaaatgatca gaccggactc atggaccaga cctttgattc gtacgaggat
     1021 ttttacaacg cgtgggatca atttgtttct aggcaagaac agggatcgat ttttcgcagc
     1081 gccaggcaca ccgaagaaga cacggggatg gctggtgctc ttgatgaggc gggggatgcg
     1141 gaggtgatgt tcccaccctg gtttgcaaag gtgaaaaaat ttttcactga tgttgaggcg
     1201 aaacgcttcc aaaacgaaga cacgatttat caggttcgtg atacaggttt tgcacaatca
     1261 cgcctgatct acatcgatgt ttatgatgaa gcacaatcca ttttcgatgt cttgttcact
     1321 gtggatcagg gtatcaatga actgttgggt gtgtacgcgg aaatggcacc agccacatcg
     1381 cttcaaacat tgccagaggg gtatgaacca gcagaccgga aagaggtcat tatgctttgc
     1441 gatcgattga agcttgacta tcaggtagtc gaggagatgt gggctgatat tgtcgcagaa
     1501 ggaatcacca cagccgatga agtgctggac gagcttaaag cccgtgccgt ggcttttgcg
     1561 caggacaggg tggtctcctt tgcctgcaaa ttggtgtcat tgatgaaagg aaaaaaatac
     1621 acaccgggag ggaaaagccc tcgtcagggt ttcgattgca gtgggttggt gtggtatgtt
     1681 tacaagtcgc ttgggctaga ttatactttt ttgggtgcca acagtttttc gcagtccgca
     1741 cgggatccga caggtgcgtt tgccaaagta actgatgatg tgcgacttgg agacgtggtg
     1801 tggtttccgg gacatgtggg ggtagtcacg agtgtggcca acgggcgggt tcaattcgtt
     1861 cattcatgca gtcggggagt ggtcacagat gatctggaag agtacatcaa atacagtccc
     1921 gaaggtggaa aacatttctt ttacaggaac agggctttgt tttaggacgg aaaaaaagaa
     1981 ttacgaacat aatgatgaga gatgtcctca aagcatagag ggcaggattg atctctgttt
     2041 gaaataaatc tcaaaataaa gcgatatttt cctaggaaag ctggtgaaaa tagtatagat
     2101 gccctctggt tcttacagtg agaacccaga aaagggcagc tagtttgcct tttttggata
     2161 atacgcattt taaggagtaa atataatgga aaaaggtaca gttaagtttt ttaatatgga
     2221 caaaggcttt ggcttcatca aaccagataa tggtgcaaaa gatctttttg ttcacacatc
     2281 agtagtcgaa aatggtccca tcgcggaagg cgacaaggtt gaattcgaag tcggtcaagg
     2341 caaaaaaggt ccttgtgccg aaaaagttcg tcgcatatag gcaaagaaca gctccgccag
     2401 ctcatattcc gctcccgatt tcattcccat ttcatagtaa tattattatt gattggtaat
     2461 gtgtctgtgt tgttgatcga tttgatcaat gggtgttggt gtttgtcctg gccagccctt
     2521 gttcgtttgt gagcttggga attttggtcc attaactaag ggccaacagg ttggcttgta
     2581 ctaccgcgtc acccttgtgc gcaaagagac cttcgatgga gcgtctgatt ttgtctttga
     2641 ggccagcgat ctggagttca ttgaccagtg cccccaaaag cacaatgttg gcggcctttg
     2701 ggttattgat cttgatcaga atttgctgca tgtcgatgag gttataatgc ctgcgttttc
     2761 tgatggcgtt gtgaatatcg atcagctcgg ggtaggctat gtttttgaca ggatccgtgc
     2821 tcgaaattac gcaaccgtct tcactcaaaa aatccaattg tcgcaatgct tccatgggct
     2881 cggtggctac gaggtagtgc gctgtgcctt tggcaatgat gccagaatgt atgggactat
     2941 ccgcgattcg cacttgcgag gtgactgctc cgcccctttg cgccatgcca tggatctcgg
     3001 tttgtttgac ttgcaagccc acgtcacaag cggcctgaat gagcatgttg gtcatggaca
     3061 atattccctg acccccaacg ccggcgaaga caatatcgta tttcatagca ctcccgttta
     3121 tcgtgttcaa gtgttcacgt gttcacgtgt tcatgtatgc tcgtaagctt gttatttctg
     3181 cagacactcc ctgcgtgcca aaatcacaga aggtccttgg tattcgcact cttcccggat
     3241 gatgcgtgta ttttcagcgt gatttttggg aatcgggtgg atgattttta aatgatcccg
     3301 atcaatcccc attcccaaaa tgaacgaaac aaactgctct gctcccatga ttctttcctg
     3361 tccccccgtc atggcgacca atgaattatc cagaatgatc acagtgaaat tgacctggcg
     3421 cgaaatgcct tcgagtaaag gtgtgatgcc ggaatgttca aaggtggaat caccgatcac
     3481 ggcgatggcg tatttcagtc cattttcgcc cgcggccttg gccatggata ttgaggcccc
     3541 catgcagacc ccggaatgaa tcgcttcgtg aggagggtag taccccagag agtaacaacc
     3601 aatgtccgaa aaaacaaccc gttcgtgttc atgacccctc agggcctcgt ttagggcctt
     3661 gaacgtgtcg gtgtgaggac aacccttgca cagttgtggt gggcgtgggc ggaccagttt
     3721 atcgtttgtc gctggtgtgg ttgttggcgt cggatacccc agactttttt tgaccagatc
     3781 ggtgttgagt tctccggttt gtgggacggt tttgtcgtgg cgtccatgca cgatgatcct
     3841 gtgagggatt tcgtgcagac attcatagat cagcgtttcg ataagggggt agccttcctc
     3901 aatgacgaac aattcgtccg cgttttgcaa aagggccaga attttctgtt tgggaagagg
     3961 ataggaacaa acgcgcagat aagaataatc atccgcacca ccgcactcca tgaaatattg
     4021 atgggccagc ccgcctgtga tgacggcctt ttgggtgttg ggtctgatgt ttaaaacgtt
     4081 cttttcgaaa tgattcgaaa actgcgtcag gtcgttttgt ttggaccata ggagttcaaa
     4141 ctgtggtctg gcattgctgg ggagcagcat ccagtttttg aaactggcag ggagtgtgat
     4201 ccgtttctga gggtgattta tttctgacaa tgttgcgttg gagcgactgt gggcgacgcg
     4261 ggtggtcagt cggatcatga tgggcaaatg ccacttttcg gaaagagtaa aagccagcag
     4321 ggtcatgtcg taggcctctt gggcgtcgat gggttccaaa caaagaattt tggagaacca
     4381 actgtaaaaa cggctgtctt gttcgttttg agatgaatgc atcgatgggt catcggccac
     4441 gcagatcacc agaccaccct gagtacctgt cagagaggca ttcatgaagg gatctgcggc
     4501 aacattgagc cccacatgtt tcatgcttac c
//
LOCUS       DATDUN010000744         3233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_615857, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000744 DATDUN010000000
VERSION     DATDUN010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615857"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1041
                     /gene="dctP"
                     /locus_tag="VJC18_10415"
     CDS             58..1041
                     /gene="dctP"
                     /locus_tag="VJC18_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HLD45838.1"
                     /translation="MFKKTIGVLVFSLVLLLASSQAQAVELKLATLAPQGTSLYNSFE
                     SLAKDISTKTGGRVTVKVFGGGVQGDEKDVVRKMRYGQIDIAALTGVGLGMIDPQVRV
                     LELPFLFGSTAEVDKVYAGMEDYFKKAFLAKGFELLGWAEIGFVKIFTNKPIQKKDDL
                     KGIKMWMWEGDPLAKAMYDAMGVTPVPLAVTDVLTSLQTNLIDACYAPEFSAIGMQWH
                     TKTKFMTDVNLVNGTGGIIVTNQSWQKIPAGDRAMIQQLVHAAARKIVEQTRVDNASA
                     KVAVQSSGIKVVNIDSAALVELQEIGKTVREQLTGSLYSAELLTKVLALLK"
     gene            1038..1610
                     /locus_tag="VJC18_10420"
     CDS             1038..1610
                     /locus_tag="VJC18_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HLD45839.1"
                     /translation="MTIVMKKFFSKIEKGLFVTEKTLLCVCLTVMLTVAALQVFLRNV
                     FDTGISWAEVFTRHMVLFLLFFGASLATRRKTHLQMDLSHKLMPACVKPVFEVFIFII
                     CIAVNSLLCKAAWVFMMGERESGSVLFYQIPTWCFIVIMPIGFGLISLRFVINFIQTI
                     HDLILRRKPLPPEFSIPEVAILCSKEDDDS"
     gene            1607..2875
                     /locus_tag="VJC18_10425"
     CDS             1607..2875
                     /locus_tag="VJC18_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HLD45840.1"
                     /translation="MKTLLITFFALIGTPLFVMFGGITLYLFMHEGVNTAVIMIEFYR
                     LASAPTLSAIPLFTFAGFILAESGAPKRMVDLAQSIIGSVPGAMPVVVILICAFFTAF
                     TGASGVTIIALGGLLYPALLKAKYHDRFAIGLVTSCGSIGLLLPPSLPLILYGLIGQV
                     NIDKLFIAGLLPSFLIFTLLALYSYFVSRKQKTERVCFEFSYVWKCIKEAKWEIPLPI
                     IVLYGIYGGIITPSEAAAVTAVYVVLVEVFIKKEIRPFKDVPRIMVESITMVGGILII
                     LGVAMGLTSYLVDHEVPTAILEWVRPHITSQAMFLLMLNVFLLIVGCLMDIFSAIIVV
                     VPIITPIALQYGVDPVHLGIIFLTNLEVGYLHPPLGLNLFIASFRFKRSLFELYWATF
                     PFLIIVLISLVVITYWPWLSTGLVELFAGH"
     gene            complement(3035..>3233)
                     /locus_tag="VJC18_10430"
     CDS             complement(3035..>3233)
                     /locus_tag="VJC18_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HLD45841.1"
                     /translation="KIPIAAKICSVADAFDAMTSNRPYRKGINPLQAANIFEQELGSG
                     QWEPDIVNTFIKLVRASYKEK"
BASE COUNT          694 a          725 c          879 g          935 t
ORIGIN      
        1 gaaaagaaca tgaagagact ttttgaaagt gtggaataat caataaggag tgatgatatg
       61 tttaaaaaaa caattggcgt attggtgttt tcattggtgt tgttgctggc ttcctcgcag
      121 gcccaggcgg tcgaactcaa actggcgact ttggccccgc aagggacttc gttgtataat
      181 tcgttcgaat ccctggccaa ggatatctcg acaaaaaccg gtggtcgtgt gacggtcaag
      241 gtttttggtg gtggagtgca gggtgatgaa aaagatgtgg ttcgtaaaat gcgttatgga
      301 cagattgaca ttgcggcctt gacgggtgtg ggacttggca tgattgaccc gcaagtccgt
      361 gtgctcgaac tgccgttcct gttcggttcg acggctgagg tcgataaggt ttatgccggt
      421 atggaggatt atttcaagaa ggcttttttg gccaagggct ttgagcttct gggttgggcg
      481 gagattggtt ttgtcaagat tttcacaaac aagcccatac agaaaaaaga tgatctgaag
      541 gggatcaaaa tgtggatgtg ggaaggagac cccttggcca aagccatgta cgacgcgatg
      601 ggtgtgaccc ctgtgccttt ggcggtgacc gatgtgctca catcgttgca gaccaatctc
      661 attgatgcct gttatgcccc ggagttcagt gcgattggca tgcagtggca caccaaaaca
      721 aagttcatga cagatgtgaa cctggttaac ggcacgggcg ggatcattgt caccaatcag
      781 tcctggcaga aaattccggc aggcgataga gcgatgattc agcaattggt tcatgccgcc
      841 gcgagaaaga ttgtcgaaca gacacgtgtt gataacgcct ctgccaaggt cgcggtacag
      901 agttcaggta ttaaggttgt caacattgat tctgcggccc ttgtcgagtt gcaggagatt
      961 ggcaagaccg ttcgagaaca gttgacgggg tctctttatt cggcagagtt gttgactaag
     1021 gtgttggcgt tattgaaatg accatcgtga tgaaaaagtt tttttcaaaa atcgaaaaag
     1081 gacttttcgt aacagaaaaa accctgctct gcgtgtgtct gactgtgatg ctcacggtgg
     1141 cggctctgca ggtgtttttg cgcaatgtgt tcgacacagg catcagttgg gcggaggtgt
     1201 tcacgcggca catggtcctt tttttgttgt tctttggtgc cagtctggcc actcggcgca
     1261 aaactcatct gcagatggac ctctcgcaca aactcatgcc ggcctgtgtc aaaccggtat
     1321 tcgaggtgtt cattttcatc atctgtatcg ctgtcaatag tctgctgtgc aaggcggcct
     1381 gggtgttcat gatgggtgag cgtgagtcag ggtcggtgct tttttatcag atccccacgt
     1441 ggtgtttcat cgtcatcatg ccgattggtt ttggtcttat ctcactgaga tttgtcatta
     1501 actttatcca gacgattcat gacctgatct tacgcaggaa accgcttcca ccggaattca
     1561 gtatccccga agtggctatc ctctgctcca aagaggacga tgactcatga aaaccctcct
     1621 catcactttt tttgccctta tcggaacgcc gttgtttgtc atgtttggcg gcatcacgct
     1681 ttatttgttc atgcatgagg gtgtgaatac ggccgtgatc atgattgagt tttaccgttt
     1741 ggcctcggcg cccacgttga gcgccatccc tctgttcacg tttgccggat tcatcctcgc
     1801 cgaaagtggt gctcccaaac gtatggtgga tttggcgcag tccatcatcg gttcggttcc
     1861 cggggccatg cctgttgtgg tgattctcat ttgcgcattt tttacggcct ttacgggtgc
     1921 cagtggagtt acgatcatcg ctttgggcgg gttgttgtac cctgcattgc tcaaggccaa
     1981 atatcatgac cggtttgcca tcgggctcgt tacgagttgc gggagtattg gtctgctcct
     2041 gcctccgagt cttcccctga tcctttacgg cctgatcggg caggtcaata ttgacaaact
     2101 tttcattgcc ggactgctcc cctcattttt aatttttacg ttgttggctt tgtacagtta
     2161 ttttgtgagc cgcaaacaaa agacagaaag agtttgcttt gagttttcgt atgtctggaa
     2221 atgcatcaaa gaggccaagt gggaaatccc tctcccgatc attgtgttgt acggtatcta
     2281 cggagggatc attacaccca gcgaggccgc agctgtcacg gctgtttatg tggtgctcgt
     2341 agaggttttc atcaaaaaag agatacggcc tttcaaggat gttccccgga tcatggtcga
     2401 gtccatcacg atggttggcg ggatcctcat cattttgggt gtggcgatgg ggctgaccag
     2461 ttatctggtg gatcacgaag tgcccacagc cattctggag tgggtcaggc ctcacatcac
     2521 aagccaggcc atgtttttgc tgatgctcaa cgtctttttg ttgattgtgg gttgtctgat
     2581 ggatattttt tcggcgatca ttgtcgtggt gcccatcatc acaccgattg ccctgcaata
     2641 cggtgtggat cccgttcatc tgggcatcat ttttctgaca aatctcgaag tgggctatct
     2701 gcacccgccg ctgggactca atcttttcat tgcctcgttc aggttcaaac gttccttgtt
     2761 tgagctttac tgggccacgt ttcccttcct gatcattgtg ctcatcagtc ttgttgtgat
     2821 tacttattgg ccatggttaa gtacgggact tgttgaatta tttgccggac attgaaggac
     2881 atctctaata accaccattt tcggggacct ctaaaaagtg cccagatgca aggcgcccgc
     2941 aaacccgcaa acggagcgta cacggaagta cgtgagtatt gcgggtgcag tgggcaacgc
     3001 cgcagatggg tagtttttag aggtcccctg aaccctattt ttctttgtat gacgctcgga
     3061 ccaacttgat aaacgtgttt acgatgtcgg gttcccattg tccggagccc aactcctgtt
     3121 caaaaatatt ggcggcctgc agagggttga tgcctttacg gtagggacga ttgctggtca
     3181 tggcgtcgaa ggcgtcggcg acagaacaaa tttttgccgc gatggggatt ttg
//
LOCUS       DATDUN010000745         1556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_496081, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000745 DATDUN010000000
VERSION     DATDUN010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496081"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            642..1451
                     /locus_tag="VJC18_10435"
     CDS             642..1451
                     /locus_tag="VJC18_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45842.1"
                     /translation="MFTSPSLLVSPGITSFGVNGQGRQPSRVDLLLQTNLSGTTPSSC
                     FVSGDEGGGGTPPPQLHHSLFSGTYYIGGQRYTPTERQAEAIKTLRAQLKHQPNHREE
                     EIDRLISIIENPVGFVSTDDVKMMVRLYAPLRITHQIVDEMDRYEALAFDDARDPARL
                     VADALDHTMVFDAIIRDVDAEGHPVELTETEYGQVSHLDSRQLFSDFSARTAAIAKVR
                     GIDSPVKNAKSNTDARNVTLKLIDYFHNVVALSRRNFAQAKKNNSIHACPV"
BASE COUNT          448 a          362 c          352 g          394 t
ORIGIN      
        1 aaaaagaaca agccgactag ggctttatta atacaggact cagtaatact ttccgtttgt
       61 ttgacacagg cctttggtac aagtgggtct gcattcccac gcaacacagt cagttgtgta
      121 gccacatttt tgctctcgaa attcctgtcg caacgtcttt gtttgctcaa actgttgtgc
      181 gtgtgtcact cccccacagg gacgatcgtt gctgagaccc gcatcaaaac aaatacaatc
      241 ttcgtttttt tgacacgggg cactgcgtgg tttgaaaatg ttgtcgcact cggtccgaat
      301 ttgttcgcag gttttgggga ttcctgcctg ggtctgcggg acaacccccg ctgggaggca
      361 aaaaaaacaa gtaataagga gtagacacat gaatatcatt ttcatgcttg gttcactaag
      421 gtgatttgtc acaaaaagca aaacatctgt tcttcgggag tgtgtccaga ccatggtgtc
      481 aacaaaatcc gtatgatggg tgtgaatttt gccccgtact ctttagagta cgaaatcgta
      541 caaattgagt gcacatcatt ttcattccaa ccgatcatta acctgacaat cgaagttcac
      601 tcgaagtcat agaacaaaca caacaataaa aaaggagtcc catgtttacc tcgcccagtt
      661 tattagtcag tcccggaata acatctttcg gcgttaacgg ccaaggaaga caaccctctc
      721 gtgtagattt gttactacaa acaaatctgt caggaacaac cccttcgtct tgttttgttt
      781 cgggtgacga aggaggcggc ggaacaccgc cccctcaatt gcaccattcc ctattttctg
      841 gaacgtacta cataggcggc cagcggtaca caccaacaga gcggcaggca gaggccatca
      901 aaacgttacg agcccaactc aaacatcaac ccaatcaccg tgaagaagaa atcgatcgtt
      961 tgatctctat cattgaaaac cccgttgggt ttgtttcaac agatgatgta aaaatgatgg
     1021 taaggttata tgcgccctta agaataactc accaaatcgt agatgagatg gacaggtatg
     1081 aagctctggc atttgacgat gcaagagatc ctgcccgact ggttgccgac gcgcttgatc
     1141 ataccatggt cttcgatgct atcatccgcg acgttgacgc agagggacat cccgtggagt
     1201 taaccgagac agagtatgga caggtttctc acctagattc aagacagcta ttcagtgatt
     1261 tttctgcgcg tacggcagca attgcaaaag tgcgcggcat cgattcgcct gtgaaaaatg
     1321 ccaagagcaa cacagatgct cgaaacgtca cactcaaact gatagactat ttccataatg
     1381 tcgtggcatt atcgagaagg aatttcgctc aggcaaaaaa aaataactca attcacgcct
     1441 gtccggttta attcacatag gtagtaggag ctgcaaatca ggtggatagc gagttgtacg
     1501 agggagatcc ccgagaagtc ttataagagg ctcttttaaa agaagcatgg tcgcca
//
LOCUS       DATDUN010000746         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_158055, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000746 DATDUN010000000
VERSION     DATDUN010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158055"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2017)
                     /locus_tag="VJC18_10440"
     CDS             complement(<1..2017)
                     /locus_tag="VJC18_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HLD45843.1"
                     /translation="MKINKVAVLGSGVMGCGIAAHLANAGVPSLLLDIVPPEFTEADA
                     KAGLTKDSKEFRNKFALNALNNVLPKQKPSPLFDASVLKLITPGNFDDDMAKLAECDW
                     VIEVVVERLDIKQKVFANVEKHVRPGTIISSNTSGIKLADMIEGRGADFAKHFIITHF
                     FNPVRYMKLVEIVSCDKTRPEVVTFMAEYLEDKLGKGVVYAKDTPSFIANRIGIYGFM
                     AIMHTAIVKGLTVEAVDKITGPATGKPKSATFRTGDLVGLDTLAHVSNNNYAMLPNDE
                     CRDAFKLPAIVTTLVDKKWLGDKTGGGFYKKTKGADGSKVILAVDLKTGEYREQGKVR
                     YDSLGAAKNAETAGDRIKAMVGSTDEAGVFAWETLRDVAIYSANRVPEIADDIVNIDN
                     AMKWGFNWDLGPFEVMDAIGVQAFAERAQKDGKALAPIITSVLKEGEGSFYKTVSGVR
                     YYFDPTSKKYLPVKLRPTSLVIQTLRAQNKVLKSNSGASVIDLGDGVVNVEFHTKMNA
                     IDGDIGEMLNVAIDLVESSPVYKGIVVSNDAPNFSVGANLMLLWLESQQKNWDSIIKL
                     VKGFQDVCMRLKYSKKPVVAAPAGMALGGGCEVIMGCDAVRAYSETYIGLVEFGAGLI
                     PGGGGNKNMILNQEATLLEKGATGWAGKSDGGPFPKIQKAFEQIGFAK"
     gene            complement(2080..2475)
                     /locus_tag="VJC18_10445"
     CDS             complement(2080..2475)
                     /locus_tag="VJC18_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006510110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLD45844.1"
                     /translation="MNNEDQKKISSVRDLDVYERAFSCAMEIFRITKEFPLEEKYSLT
                     DQIRRSSRSVCTNISEGWRKRKYKAVFINKLSDAAQEASETQTWLEFSLACKYIAKDT
                     FVRIDEEYEHVFAQLNTMERKAEKFCFGS"
     gene            complement(2522..>2617)
                     /locus_tag="VJC18_10450"
     CDS             complement(2522..>2617)
                     /locus_tag="VJC18_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45845.1"
                     /translation="VHGLHHYGKKYGVESMCVGGGQGAAVVVERM"
BASE COUNT          615 a          748 c          590 g          664 t
ORIGIN      
        1 ccttggcgaa tccaatctgc tcaaaggctt tttggatttt ggggaacggc ccgccatcgg
       61 atttacccgc ccacccggtc gcaccctttt ccaaaagagt ggcttcctga ttgagaatca
      121 tgttcttgtt gccgccaccc cccggaatga gccccgctcc aaactccacc aaaccgatgt
      181 aggtctcgga gtacgcgcgc acggcatcgc aacccatgat gacctcgcac ccgccaccca
      241 aggccatgcc tgcgggtgcg gcgaccacgg gttttttgga atatttgaga cgcatgcaca
      301 catcctgaaa acctttgacg agtttgatga tgctgtccca gttcttctgt tgcgactcca
      361 gccacaaaag catgaggttg gctcccacag agaaattggg ggcgtcattg gacaccacaa
      421 tccccttgta cacagggctg gattccacca gatcaatggc cacattcagc atttcgccaa
      481 tgtcgccatc gatcgcgttc atcttggtgt gaaactcaac attcaccacc ccatcaccca
      541 gatcaatcac ggacgcgcca gaattggatt tgaggacctt gttctgcgcc cttaacgtct
      601 gaatgaccaa cgatgtgggt cggagcttga ccggcaggta ttttttgctg gttgggtcaa
      661 aataatacct cacgccactg accgtcttgt aaaagctccc ctcgccttcc ttgagcacgg
      721 acgtgatgat cggcgcaagc gccttgccat ccttctgggc acgttcggca aacgcctgaa
      781 cgccaatcgc atccataacc tcgaacggcc ccaaatccca gttgaatccc catttcatgg
      841 cgttgtctat attcacaatg tcatcagcga tttcaggaac acgattggcg gaataaatcg
      901 ccacatcgcg cagggtctcc cacgcaaaca caccagcctc atcggtggaa ccgaccatgg
      961 ccttaatgcg atcacctgct gtctccgcat tcttggcggc ccccagactg tcgtagcgga
     1021 ccttgccctg ttcgcggtac tcaccggtct tgagatcgac cgccagaatc accttgctcc
     1081 catcggcgcc cttggttttt ttgtaaaaac caccgcccgt tttgtcgccc agccattttt
     1141 tgtcgaccag tgtggtcaca atggcaggga gtttgaaagc gtcgcgacac tcatcgttgg
     1201 ggagcatggc ataattgtta ttggacacat gggcgagcgt atcgagcccg accagatcac
     1261 ccgtacggaa cgtggctgac tttggtttgc cagtggctgg ccccgtgatt ttatcgaccg
     1321 cctccactgt gagccctttg acaatcgccg tgtgcatgat ggccatgaac ccgtaaatgc
     1381 caatgcggtt agcaataaag ctgggcgtgt ctttggcgta caccacaccc ttgccgagtt
     1441 tgtcttccaa atattcggcc ataaaggtga ccacctcagg acgggttttg tcgcacgaaa
     1501 caatctcaac cagtttcatg tagcgcacgg ggttgaaaaa atgcgtgatg atgaagtgct
     1561 tggcaaaatc tgctccgcgc ccttcgatca tgtcggcgag cttgatgccc gacgtgttgg
     1621 aagaaatgat tgtgccaggc cgcacatgtt tttcaacatt cgcgaagacc ttttgtttga
     1681 tgtcgagccg ttccaccacc acctcaatca cccaatcaca ctcggccagc ttggccatat
     1741 catcgtcaaa attccccggc gtgatgagtt tgaggactga ggcgtcaaac aacggtgacg
     1801 gtttttgttt gggcaagaca ttgttcaggg cattgagcgc aaacttgttg cggaactctt
     1861 ttgagtcttt ggtgagcccc gccttggcat cggcctccgt aaattctgga gggacgatgt
     1921 cgagcaacaa cgacggcact cccgcgttgg ccagatgtgc cgcgatgcca caacccataa
     1981 cccctgagcc gagaacggcc actttattga tcttcataga aatactccgg tttagttcaa
     2041 caatcgaaaa cacgagggac gaaaacacga gggacgacat caacttccaa aacaaaattt
     2101 ctctgctttt ctttccatgg tattgagttg agcaaagaca tgttcatact cttcatcgat
     2161 tctcacaaaa gtatctttgg caatatactt gcatgccaat gaaaactcta accacgtctg
     2221 cgtttctgaa gcttcttggg ccgcatccga caatttattt atgaaaacag ctttatattt
     2281 tctttttctc cagccttcag aaatattagt acaaacagaa cgggatgacc tgcgaatctg
     2341 gtccgtcaaa gagtactttt cctctaacgg aaattccttt gtgattcgaa aaatctccat
     2401 agcgcaagaa aatgcccttt catagacatc gagatccctc acactactga tttttttttg
     2461 atcttcgtta ttcatcaata gccaacctcg tccctcgtgt tttcgtgttt tcgtgttctc
     2521 attacatacg ctccaccacc accgccgcgc cttgcccacc gccgacgcac atggattcga
     2581 caccatactt tttgccataa tgatgtaatc catgaac
//
LOCUS       DATDUN010000747         1599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_289746, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000747 DATDUN010000000
VERSION     DATDUN010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289746"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1255
                     /locus_tag="VJC18_10455"
     CDS             335..1255
                     /locus_tag="VJC18_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLD45846.1"
                     /translation="MSPLTINKIPHHVFLAHKDFSTELLDELSAVVQQLDRLVIAKPQ
                     PSIWAQDVWRDVVEIKFESISQAIQALRALGKNWIFFPHKFYRRANLIQEGLQTVAVN
                     RIPFPNLKPIQPFGCWTLHDANTILASTNTQKNVPLGAYEFIENKQIPPNRAYLKLWE
                     VFTSLNFFPDKKSLCLDLGASPGGWTWVLQSLGCRVIAVDKAELDPKIGRLANVTAVK
                     QSAFALDARHHEPVNWLFSDVICYPQKLYTFVSRWIEFGKCQNFVCTIKLQGKTDNAM
                     LKKFLDIPNSQILHLYHNKHEVTWVRRVAQ"
BASE COUNT          435 a          375 c          335 g          454 t
ORIGIN      
        1 ttcctcacac aaaaagaaag aaacgttctt tctctggaga ataacatttt caaagcggcg
       61 caaatcccga aggacttgag tatctgccag aagttttgtt tgttaatgaa accgctcatg
      121 actactacat atgcatcgga cgtgccaatt cagagagatt tttagttcat tgaaatcata
      181 agtatttcgg tttcgggtcg tcccaataac tgtcccattt tggagacagc atgtctcaac
      241 tgtccaattt tttggggggt gttgtcgcga tattgataga ttgattgata aaaattgtta
      301 actcctgtca attcatgtaa tacgacgaac gcgaatgagc cccctgacca tcaacaaaat
      361 ccctcatcat gtttttttgg cccataaaga tttttctacc gaacttctcg atgagctcag
      421 tgccgttgta caacaattgg accgactcgt aattgccaaa ccgcaaccct caatttgggc
      481 tcaggatgtc tggcgagacg tggtggaaat caaatttgaa tctatctctc aggctataca
      541 agccctgcgt gcactgggca aaaactggat tttttttcct cacaaatttt acagacgggc
      601 taacctcatc caagaaggac tccaaacagt tgctgttaat cgcatcccgt ttcccaactt
      661 gaaacctatt caaccttttg gctgttggac gctccatgat gccaacacaa ttctcgccag
      721 caccaacact caaaagaatg tccctttggg tgcttatgaa ttcattgaaa acaaacaaat
      781 cccgcccaac cgcgcgtatt taaaactttg ggaggtcttc acctcactga atttttttcc
      841 agataaaaaa tcgttgtgtc ttgatctggg tgccagcccg gggggatgga cttgggtttt
      901 gcaatcattg gggtgtcggg tcattgccgt tgacaaggca gagctcgatc ccaaaattgg
      961 caggctcgcc aacgtaacgg ccgtcaaaca aagcgcgttc gctcttgatg cccgtcatca
     1021 tgaaccagtg aactggctgt tcagtgatgt gatctgttat ccccaaaaac tttacacgtt
     1081 tgtttcgaga tggattgaat ttgggaaatg tcagaatttt gtgtgcacca tcaaattgca
     1141 gggcaaaacg gacaatgcca tgctgaaaaa atttttggac atcccaaatt cacaaattct
     1201 gcatctgtat cacaacaagc atgaggtgac ttgggtacgg cgagttgccc aataaaccgc
     1261 cgtccccaag tgccattggg tcgttgtttt gaaggcgtca gaaaaaatca acacacgtga
     1321 caaaacatga atcgttgatt tttgatggtt caaagaaaat gacaagcggt cgttttcttt
     1381 gaacggcctg aagaacaatg acccaatggc actatttaac ttatgtctca acaaaaacgc
     1441 ttcatcctct atttcatcat cctgggcacc ttctggggag ggtcctttct ggcgatcaag
     1501 tatgccgtgg ctggccttgg tacccacttt gccatttttt tgagggtgtc aattgccctg
     1561 ctcttcctgc gcattttcgt catacacaaa gtccctaaa
//
LOCUS       DATDUN010000748         1978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_201935, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000748 DATDUN010000000
VERSION     DATDUN010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201935"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VJC18_10460"
     CDS             <1..547
                     /locus_tag="VJC18_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HLD45847.1"
                     /translation="RVDQHQRSIGKTINFGVVYGQSPFGLAQQLGIPQKEARRFIDSF
                     YNEFSRVRDYREQVLARGRERGYVSTFLGRRRYLPDINSKNFMARQNAERMAFNAVFQ
                     GSAADLIKKAMIVIHHWIIKDQLKTKMLLQVHDELLFEVPQEELTVIRKFIPEMMERA
                     FSLSVPLKVSCHVGKNWAEAH"
     gene            complement(551..745)
                     /locus_tag="VJC18_10465"
     CDS             complement(551..745)
                     /locus_tag="VJC18_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45848.1"
                     /translation="MQRNFLCLCSAISLQIILYTVLLFTACHKPASTQQSLADVTLGA
                     STNPVADETCKPETEKPCDE"
     gene            complement(813..886)
                     /locus_tag="VJC18_10470"
     tRNA            complement(813..886)
                     /locus_tag="VJC18_10470"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(850..852),aa:His,seq:gtg)
     gene            1128..>1978
                     /locus_tag="VJC18_10475"
     CDS             1128..>1978
                     /locus_tag="VJC18_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HLD45849.1"
                     /translation="MCGIVGYIGDKKAKPILIDGLKRLEYRGYDSAGLAFLQNGEFEI
                     LRTQGKLQNLIDKINGREFSATIGIGHTRWATHGKPDEINAHPHYSQDVVLVHNGIIE
                     NYKPIKDRLITKGHNIVSETDTEIICHLIQDYLNQDNSFITSLQKTLKELRGAYSLVM
                     LDRKDNSRIYCARKGSPLVLGLKEGEKFVASDIPALLTHTNEVMFLHDMELAVLEKNK
                     VTVYDFEGNTVVKTSQIIQWTMAQAEKSGYKHFMLKEIHEQPRVIADTLLGRVDLEKL
                     NIWLDDAG"
BASE COUNT          574 a          444 c          474 g          486 t
ORIGIN      
        1 aagggtggac cagcaccagc gaagcatcgg taaaacgatc aactttggcg tggtgtatgg
       61 ccaatcgcct tttggtctcg cacaacaatt gggtatcccg caaaaagagg ccagaagatt
      121 tatcgacagt ttttacaatg agttttcacg cgtgcgggat tatcgggaac aggttttagc
      181 gcggggcaga gaacgggggt atgtcagcac gtttttggga cgtcgccgtt atttgccaga
      241 catcaacagc aaaaacttta tggcccgcca gaatgccgaa cgcatggctt tcaatgccgt
      301 gtttcagggt tctgcggcag atcttatcaa gaaggccatg atcgttatcc atcactggat
      361 catcaaagat cagctaaaaa caaaaatgct tttgcaggtt catgacgaat tactttttga
      421 ggtccctcag gaggaactca ccgttattcg taaattcatt ccggaaatga tggaaagggc
      481 cttcagtctc agcgtgccgc taaaggtgag ttgtcatgtg ggcaaaaact gggctgaagc
      541 gcattgaaat tcattcatca caaggttttt cggtttctgg tttgcaggtc tcgtctgcaa
      601 cagggttggt gctggcgccc agggtgacat cggcgagtga ttgttgtgtg cttgcgggtt
      661 tgtgacaggc agtgaagaga aggaccgtat acaagattat ttgaagactg atcgctgagc
      721 aaagacagag aaaattacgt tgcatagaaa attgaaattg gaaaatagag gagttgcaaa
      781 gaatctcact taggtgagat tctttgcaac tgggtgagtg aggggacttg aacccccgac
      841 ccccagggcc acaacctggt gctctaacca cctgagctac acccaccata aaacacaaga
      901 gcggctctac taatcaaact tggatcaaaa agtaaaccaa attcacgggc atgcttttta
      961 tttgaacaaa ctggtgcttt tgactaatct gtgctaccga cattcgatcc aatgttgttt
     1021 cgataacagt cgaatttatt gagcaaaaaa ggtctgttgt tgcctttggt tgtccttttc
     1081 gtatttgtgt tgtgaacacc agaaaccagc aacgaactac aaagtctatg tgcggcatag
     1141 tcggttatat tggcgataaa aaagccaaac caatactaat tgatgggctg aaaaggcttg
     1201 agtaccgcgg ctacgattca gccgggctgg cttttctgca aaatggagag tttgaaatcc
     1261 tgaggactca gggcaaactc cagaatctca tcgataaaat caatggcagg gaattctctg
     1321 caaccatcgg tattggacac actcgctggg caacccacgg aaagccagac gaaatcaatg
     1381 ctcaccctca ttattcacaa gacgttgtgc tggttcataa tggtatcatc gagaactata
     1441 aacccattaa ggatcgcttg atcaccaaag ggcacaacat cgtttcggaa acagacacag
     1501 aaatcatctg tcacctcatc caagactacc tgaatcaaga caactccttc atcacgtctc
     1561 tccaaaaaac actcaaggaa ttacggggtg cttactctct ggtcatgtta gacaggaagg
     1621 acaattcacg aatttattgc gcacgcaagg gatctcctct cgttttgggg ctcaaggaag
     1681 gcgaaaagtt tgtagccagt gacatcccgg ccttgctcac acacaccaat gaagtcatgt
     1741 ttttacacga catggaactg gctgttttgg aaaaaaacaa agtgaccgtg tacgatttcg
     1801 aaggaaatac cgtcgtcaag acgtcccaga tcatccaatg gaccatggct caggcagaaa
     1861 aaagcgggta caaacatttc atgctcaagg agattcatga acagccacgc gtcattgccg
     1921 acactctgct tggccgcgtc gatctcgaaa aactcaacat ctggctcgat gatgccgg
//
LOCUS       DATDUN010000749         1009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_530639, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000749 DATDUN010000000
VERSION     DATDUN010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530639"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..109)
                     /locus_tag="VJC18_10480"
     tRNA            complement(36..109)
                     /locus_tag="VJC18_10480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Gly,seq:tcc)
     gene            152..>1009
                     /locus_tag="VJC18_10485"
     CDS             152..>1009
                     /locus_tag="VJC18_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HLD45850.1"
                     /translation="MCGIFGVTADRQAPHTILAGLKRLEYRGYDSWGIAVKGKDKIEI
                     EKHAGKISQSTTSLANSSFGIGHTRWATHGGATDANAHPHLDCTRKLAIVHNGIIDNW
                     QDLKKEVGNHKLVSETDSEIVAHLIEDELKTSDLEKSFIRVFKQVHGFNALIAIHQDY
                     PYIMAGKTCSPLIIGVLPGSNLIASDVSSLLPLTQKVIFLEDGQIAKISKDQIDIIDV
                     STLKKVRPHINHISWSAASTNKGKYPHFMLKEIHEQPQIVLNLLNSKKYEIDHLAEQI
                     NKSGDVYSVA"
BASE COUNT          316 a          285 c          198 g          210 t
ORIGIN      
        1 ggataatacc actctttcga aaaacgattc cgatatggag cgggtaaggg gagtcgaacc
       61 cctgtcatca ggttggaaac ctgaggtaat accgttatac gatacccgct agcaaaagat
      121 attttatcat tctgggtgct aacatataaa aatgtgcgga atctttggcg tcaccgcaga
      181 taggcaggcc cctcacacaa tactcgctgg cctcaagcgc ctcgaatatc ggggctatga
      241 ttcctggggg atcgcggtaa agggcaaaga caagatcgaa atcgaaaaac acgccggcaa
      301 aatcagccaa tcaaccacct cgcttgcaaa ctcctcattt ggcattggcc acacccgctg
      361 ggccacccac ggcggcgcta ccgacgcaaa cgcccacccc cacctggact gcaccagaaa
      421 acttgcgatt gtccacaatg ggatcataga caactggcaa gacttaaaaa aagaggtagg
      481 caatcacaaa ctggtttcag aaacagacag cgaaatcgtc gcccacctca ttgaagacga
      541 actcaaaaca agtgatctgg aaaaatcatt tatacgagtt ttcaaacaag ttcatggctt
      601 caacgctctc attgcaattc atcaagacta tccctacatc atggccggca aaacctgctc
      661 tcctctcatc atcggagttc tgccggggag taatctgatt gcctcagatg tttcatctct
      721 tctgcctctt acccaaaagg tcattttcct cgaagacggc cagatcgcca agatcagcaa
      781 agaccaaatc gacatcatcg atgtttctac tctcaaaaaa gtcagaccac acataaacca
      841 cattagctgg tctgccgcct cgaccaacaa gggcaaatac ccacacttca tgctcaaaga
      901 aatccatgag caaccacaga ttgtcctcaa cctattaaac tccaaaaaat acgaaatcga
      961 ccacctcgcc gaacaaatca acaaatcagg ggacgtctac tccgttgcc
//
LOCUS       DATDUN010000750         1586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_201980, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000750 DATDUN010000000
VERSION     DATDUN010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201980"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VJC18_10490"
     CDS             complement(<1..263)
                     /locus_tag="VJC18_10490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096719.1"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HLD45851.1"
                     /translation="MSVPIYKNWRSLIRPKFVEVDKESRSDSYAKFIARPLERGFGLT
                     LGNALRRVLLSSLQGAAITAVKYEGVLHEFSTIPGVKEDVVNI"
     gene            complement(378..1004)
                     /gene="rpsD"
                     /locus_tag="VJC18_10495"
     CDS             complement(378..1004)
                     /gene="rpsD"
                     /locus_tag="VJC18_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HLD45852.1"
                     /translation="MARYRESVCKQCRREGLKLFIKGDRCYSDKCALERREYAPGQHG
                     QGRKKSSEYATQLREKQKIRRIYGVLEKQFRKIFWEAERRKGITGENLVQLLEARLDN
                     MVYRFGFANSRNEARLLVGQRHFTVNGKIVTIPSYVTKPGDEICVREKSQKAVRIQES
                     MKAVQRRGVPEWLALDEKKMVGKIKMLPMRQQITMPMNEQLVVELYSK"
     gene            complement(1171..1548)
                     /gene="rpsK"
                     /locus_tag="VJC18_10500"
     CDS             complement(1171..1548)
                     /gene="rpsK"
                     /locus_tag="VJC18_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HLD45853.1"
                     /translation="MGKKKKKVKKNVSEGVVHIQASFNNTIITISDKAGDVISWSSAG
                     NMGFKGSKRSTPFAAQIASEEAARRALEAGVTSVVVYVKGPGAGRETALKALGHAGLR
                     VIRIQDVTPIPHNGCRPPKKRRM"
BASE COUNT          394 a          453 c          288 g          451 t
ORIGIN      
        1 atgatgttca ccacatcttc cttgacacca gggatcgtag aaaactcatg taacacacct
       61 tcatacttga cagccgtaat ggccgccccc tgtagggaac tcagcaaaac gcgtcgcaag
      121 gcgtttccca aggtcaaacc aaagcctcgt tccaagggac gggctatgaa ttttgcgtaa
      181 ctatcagagc gggattcctt gtcgacttcg acaaattttg gtctgatcaa acttctccaa
      241 tttttataaa tcggtacact catagtaaac cctcactatt tgttatgagt tatgagttat
      301 gggttatggg atatatgtga ttgagcaaat ccgccatcat tacccatacc cattacacat
      361 tacacattac acattaccta tttagaatac aattccacca ccaattgttc gttcatgggc
      421 attgtgattt gttgtctcat gggaagcatt tttattttgc ccaccatttt cttttcgtcc
      481 agagccaacc attctggaac gccccgacgt tgtactgctt tcattgactc ctgaatgcga
      541 accgccttct ggcttttttc gcgaacacaa atttcatcac ccggcttggt cacgtacgat
      601 gggatcgtca cgatcttgcc attgactgta aaatgtctct gccccaccag taacctggct
      661 tcattacgtg aattggcaaa accaaaacga tacaccatat tatccaagcg ggcttccagt
      721 aactgaacca gattttcgcc tgtaatccct ttacgtcgct cggcttccca aaaaatctta
      781 cggaactgct tttccaaaac gccatagatg cgtctgattt tttgtttttc tctcaactga
      841 gtcgcgtatt cagaactttt cttgcgaccc tggccatgct gtcccggcgc gtactcacgt
      901 ctttccaagg cgcatttgtc ggaataacaa cgatcacctt tgatgaacaa cttcaagccc
      961 tcacgacgac actgtttgca aactgattct ctgtatcttg ccataaattt acttgtttac
     1021 caatgctcgt aacctgtctc aaaaaacaag aaacagcatt gtttgataac atctcccttt
     1081 tacctaagct cccactctgt gccccacacc ctgcgccttg atccttgagc ccaaagcctt
     1141 aaaccttacg ccttgagcct taaaccttcg ttacatccgt ctctttttgg gaggacgaca
     1201 gccattgtgc gggatgggcg tcacatcctg aatgcgaata accctcaaac cagcatgacc
     1261 caatgcttta agagccgtct cacgaccagc acccggaccc tttacgtaca caacaaccga
     1321 agtgactccc gcttccaatg ccctgcgtgc cgcctcttcc gacgcgattt gcgccgcaaa
     1381 tggagtggat cgcttcgaac ccttaaaccc catattgccc gcgctcgacc aactgatcac
     1441 atcgccagcc ttatcggaaa tggtgatgat cgtattgttg aacgaagcct gaatgtggac
     1501 gacaccttca gaaacattct ttttaacctt cttcttcttc tttcccacaa cagccttgcc
     1561 acccttggtt tttttgccag cctcgg
//
LOCUS       DATDUN010000751         1658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_616043, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000751 DATDUN010000000
VERSION     DATDUN010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616043"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VJC18_10505"
     CDS             <1..1435
                     /locus_tag="VJC18_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLD45854.1"
                     /translation="KKLNEAGKTIVMVTHEMEIGAQATRIIRMRDGKIVADEILNPKA
                     VRGASINQKSVDENLKGPVIVKQQRKFLTPTELVDNFKQGFRSIFSNKLRSLLSMLGI
                     LIGVTSVIAMLALGAGATESMSERLSSLGTNVLTIRSGAHHMGGVSLESGAITRFTLQ
                     DAQAISKLDHVKNVSSSVSGRGQLVYGDKNWNTQIRGVDPAYQSIQSLQPVLGNFFVE
                     EDNTSRKKVVLLGLTVVDELFNNQNPVGKTIKINRINFEVIGVLPTKGMGPGGDRDDM
                     VIIPLTTAMYRVLGKNYTDSIDVEVDDMNWMDQTSDAIKTLIVKRHRLSEDNKDSFNI
                     MNMAEIQQALKSVTSTMTLLLGFIAVISLLVGGIGIMNIMLVSVTERTKEIGLRKAIG
                     ARSKDILFQFLTESVLMTVTGGLIGVVFGTASAKLLSYVAGWSTHVSVFSILLATVFS
                     VLVGLGFGLWPAQKAARLNPIEALRYE"
     gene            1481..>1658
                     /locus_tag="VJC18_10510"
     CDS             1481..>1658
                     /locus_tag="VJC18_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HLD45855.1"
                     /translation="MIKTKITERLHIKYPIFAGTMMNITTPEFVAVCSNAGGLGILAS
                     AIYKSPETLKEAIQL"
BASE COUNT          432 a          351 c          442 g          433 t
ORIGIN      
        1 caaaaaactg aatgaagccg gaaaaaccat cgtcatggtc acacacgaaa tggaaattgg
       61 tgcacaggcc acgcgtatca tccgcatgag agacggaaaa atcgtggcag atgaaattct
      121 gaacccgaag gcggttcgcg gtgccagcat caatcagaaa tcggtagatg aaaacctcaa
      181 agggccggtg atcgtaaaac aacaaagaaa atttctcaca cccactgaac ttgttgataa
      241 cttcaaacag gggtttcggt ccattttttc aaacaagtta agatcgcttt tgtccatgtt
      301 gggtattctc attggggtca cgtctgtcat tgccatgctg gcgttgggtg cgggggccac
      361 ggaatccatg tctgaacgcc tgtcgtcttt gggcaccaac gtgctcacga tcagatcggg
      421 agcgcatcac atggggggtg tgagcctgga atcgggtgcg atcaccaggt ttaccttgca
      481 ggatgcgcag gcaatttcca aactcgatca tgtaaaaaat gtgtcgtcat cagtctcggg
      541 gcgaggccag ttggtgtatg gggacaaaaa ttggaacaca cagatccgcg gggtggatcc
      601 tgcctatcag agcattcaat ctttgcagcc tgttttgggt aatttttttg tggaagaaga
      661 caacacgtca cgcaaaaaag tggttttgtt ggggctgacg gttgtggatg agctttttaa
      721 taatcaaaac ccagtgggga agacgatcaa gatcaaccgc atcaattttg aggtgattgg
      781 cgttttgccg acaaaaggga tggggccagg tggcgatcgc gatgatatgg tcatcattcc
      841 cctcacgacg gccatgtacc gggtattggg caaaaattat acggattcaa ttgatgtgga
      901 agttgatgac atgaattgga tggatcaaac aagtgatgcc atcaaaacac ttatcgttaa
      961 acgtcatcgt ttgagtgagg ataacaagga ttcattcaac atcatgaaca tggcagagat
     1021 ccagcaggcg ctgaaaagcg tcacgagcac catgaccctg ctcttgggat tcattgcggt
     1081 gatatctctt ttggtgggtg gcattggcat catgaacatc atgctggttt cggtgactga
     1141 acgcaccaag gagatcggtt tgagaaaggc cattggggca cgtagcaaag acattttgtt
     1201 tcagtttctg actgaatctg ttttgatgac tgtgactggt gggttgatcg gcgttgtttt
     1261 tgggacagcc tcagccaaac ttttatctta tgttgccggt tggtccacac acgtctctgt
     1321 gttttccatt ttgctggcaa cagtattttc ggttttggtg gggttgggtt ttggtctttg
     1381 gcctgcccaa aaggccgctc ggcttaatcc cattgaggcc ctgagatatg aatgaagaaa
     1441 aattcttgac ccctatttcg cgctgtgttc gacgtgactc atgattaaaa ccaagatcac
     1501 agaacggttg catattaaat acccgatttt cgctggtacc atgatgaaca tcaccacgcc
     1561 ggaattcgtg gcagtgtgtt cgaacgcggg tggcctaggg attctcgcga gtgccattta
     1621 caaatcaccc gaaacgctga aagaagcgat tcagctca
//
LOCUS       DATDUN010000752         1736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_289935, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000752 DATDUN010000000
VERSION     DATDUN010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289935"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VJC18_10515"
     CDS             complement(<1..294)
                     /locus_tag="VJC18_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45856.1"
                     /translation="MKSIYFLLSTRIACLIFLLLIVVACGSSVSQSDEPEPAVENLPE
                     GWTHDVLFTNVCASPTNMTTAADGAMYVNDRADNRIILFSSDGTLEEWADTGDV"
     gene            complement(671..1084)
                     /locus_tag="VJC18_10520"
     CDS             complement(671..1084)
                     /locus_tag="VJC18_10520"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HLD45857.1"
                     /translation="MSDDLKALEIQRERLYQKVLSFGDFRRGSISATFGKCGKKNCAC
                     AAKDHPGHGPHYRWTMTRKGKSLAQHLRLGPELEVVQKQVDQGSRFQKWYEAVIEINE
                     KICRLRPVPEIKDEHELEVLKKKLRAKFAKKSRGK"
     gene            1374..>1736
                     /locus_tag="VJC18_10525"
     CDS             1374..>1736
                     /locus_tag="VJC18_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45858.1"
                     /translation="MNKGFVLNLFFLLILSFAVHAADQTPQTQNATPLPQNVSYHLSL
                     QLDKPAQENLYSGTVVISFDLASNNRDLFLDFNSGTVVALTINDKPVTQPVYDSNTIT
                     LPARSLVTGTNHVSVMFSH"
BASE COUNT          474 a          454 c          333 g          475 t
ORIGIN      
        1 cacatcacca gtgtctgccc actcctcaag cgtgccatca gaactgaaca gaataatacg
       61 gttatctgcc cgatcattaa catacatggc tccatcagca gcagtggtca tgttggtagg
      121 actggcgcag acattcgtaa aaagaacatc gtgagtccaa ccttcaggta ggttttcaac
      181 agcgggctct ggctcatcac tttgtgaaac gcttgaacca cacgccacaa caatcaacaa
      241 caaaaaaatc aaacaggcaa tacgagttga aagtaggaag taaatcgatt tcataagtta
      301 ttatttttcc ataacactgt tttgtcaaat tattaagagg ctctctaaac attagccttc
      361 attagccttc attagcattc attagcattc atgacaaagg tgcaaggctg cattttccaa
      421 catccaaaaa gttgctcttg gctttatttc cttattattg atggtgtttt ctctactcca
      481 caaacatcgt cggatcaacc ggctcgtccg caaagaacac cgcaatggcc tccgtcatca
      541 gcgttgacaa gtttttccaa caacgtcgcg cccaccttgt gcatggaagt gcgcacagac
      601 atctcagacg cttctaaatc caagcctaca ccgttagctt gatcaagcag tatcaggtga
      661 caaagacgat tcacttcccg cgtgacttct tcgcaaactt cgcgcgcaat ttttttttaa
      721 gaacttccaa ttcgtgttcg tctttgattt ctggtacagg tctcaaacga caaatcttct
      781 cgttgatttc aatcaccgcc tcataccact tttgaaaacg ggatccctgg tcgacttgtt
      841 tttgcacaac ctccaactca ggtcccaaac gcaagtgttg agccaaactt ttgcctttcc
      901 gtgtcattgt ccaacggtaa tgtggcccat gccctgggtg atccttggca gcgcaagcgc
      961 agtttttttt tccgcatttg ccaaatgttg ccgagataga tcccctgcga aaatcaccaa
     1021 aggagagcac cttttgataa agacgttctc gttgaatttc gagtgctttt agatcatctg
     1081 acatagacac ctctttctta atgttaagca gcattaagat tatgaggcaa aaaaaagagg
     1141 aagtcaatct cgaaaaatct ttcaaattgg ccgtattttc tacctccccc gccctacggg
     1201 cacccccgcc ttacaacagg cggggggggg caaaatcaaa tttacctact tttatgtcgt
     1261 acacccgatt tgtggggaat gttgactttg tgaatccttt tgtatcatag tgaaacccgt
     1321 accccttcga gatcacaagt ggtcccggag gaggctaaat gaatggggga gtcatgaata
     1381 aaggatttgt ccttaacctg ttttttttat taatcctgtc ctttgctgtt cacgccgccg
     1441 atcaaacacc acaaacccaa aacgcaaccc cattgcccca aaatgtttcg tatcacctca
     1501 gcttgcaatt ggacaaaccc gcacaagaaa acctctattc cggaacggtc gtgatctctt
     1561 ttgacctcgc cagcaacaac cgcgatttgt ttttggattt caacagcggt acggtggtgg
     1621 ccctcaccat caatgacaaa ccagtcacgc aacccgttta tgattcaaac acaataacac
     1681 ttcccgcccg aagtttggtg acgggcacca atcacgtgag cgtcatgttc tctcac
//
LOCUS       DATDUN010000753         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_202168, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000753 DATDUN010000000
VERSION     DATDUN010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202168"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VJC18_10530"
     CDS             <1..191
                     /locus_tag="VJC18_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45859.1"
                     /translation="FTNTLAFEYCELGLHLWYMNDRSTTVKRKNDPRNRRTATTQSPV
                     EPHRARTVHCGEHAQRHV"
     gene            complement(235..308)
                     /locus_tag="VJC18_10535"
     tRNA            complement(235..308)
                     /locus_tag="VJC18_10535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Arg,seq:cct)
     gene            complement(299..796)
                     /locus_tag="VJC18_10540"
     CDS             complement(299..796)
                     /locus_tag="VJC18_10540"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HLD45860.1"
                     /translation="MKNGEKNKPTLGSFSDERVLDVCRLLNDEGAEYLVIGGIAVQLH
                     GVMRLTKDIDLLIPRDEKNAEKVIEALTNIGFGIARELSAEEVVKKPFTIIGDIPRVD
                     LITVANKIKYSEAVETALQAKVDGVKIPYVGLDILIQTKKTNRHQDKSDIEDLEMVSK
                     KKKLS"
     gene            complement(753..1208)
                     /locus_tag="VJC18_10545"
     CDS             complement(753..1208)
                     /locus_tag="VJC18_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLD45861.1"
                     /translation="MLEALFGNKTAEKIFFHLYEKRRASAYEISKCCDIPLSMVQKQL
                     MRLESGGILRSRFIAKKREYRWDPLWPFYRAFQRFLGFKKKLLREQLLDPADGSYLTI
                     EERLALGESFFRQGMKLCPVKPYQPFVKSFDSYRDYEKWRKKQTHPWLI"
     gene            complement(1283..1795)
                     /locus_tag="VJC18_10550"
     CDS             complement(1283..1795)
                     /locus_tag="VJC18_10550"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HLD45862.1"
                     /translation="MNLSKIIRDIPDFPKKGILFKDITTLLKDATAFKATIEQMTALY
                     AGKGITKVCGAESRGFIFGAPLALALNAGFVPIRKPGKLPAAKISQEYTLEYGTDKIE
                     MHVDAVAKGEKVLIVDDLLATGGTAKACAQLLSQVGAEIVGFVFLVELDFLKGRDLLK
                     GYEITSLVHY"
     gene            complement(1887..>2281)
                     /locus_tag="VJC18_10555"
     CDS             complement(1887..>2281)
                     /locus_tag="VJC18_10555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLD45863.1"
                     /translation="RNSGDLEMIWPVKEFHLTSGFGMRRGRPHDGLDLSAKIGTPILA
                     AAEGKIIFEKKFAGYGNLIVIKHAKNFFTAYAHCHKTNVTVGKTVKQGQQIGSVGRTG
                     RASGPHLHFEIHKGVEAVDPVLYLPKKT"
BASE COUNT          612 a          608 c          448 g          613 t
ORIGIN      
        1 attttacgaa tactcttgcc tttgaatact gcgagttggg tttgcatttg tggtatatga
       61 acgatcgttc aacaacggtc aagagaaaaa acgacccaag aaacaggcgc acagcaacaa
      121 cacaatctcc tgtggagccc cacagggctc ggaccgtgca ctgcggtgaa catgcccaaa
      181 ggcatgtttg acgctgtgcg ccccagactt cgcaaagaca ctttttcttg taatcgctct
      241 caagaggatt cgaacctctg acctacggct taggaagccg ccgctctatc ctgctgagct
      301 atgagagctt tttcttttta gacaccattt ccaaatcttc tatatcgctc ttgtcctgat
      361 gccgatttgt tttcttcgtc tgaatcaaga tatccaatcc aacgtacggg attttaacac
      421 catcaacctt agcctgcaat gcggtctcta cggcctccga atatttgatt ttgttcgcca
      481 cggtaatcaa atccactctg ggaatatcgc caatgatcgt aaaaggcttt tttacgacct
      541 cttcagccga aagctcacgc gcaatgccaa acccaatgtt ggtaagagcc tctatgactt
      601 tttcggcatt tttctcgtct ctcggaatca aaagatcaat gtcttttgtg agtctcatca
      661 caccatgcaa ctgtacagcg atgcccccga tcacaagata ctcggcacct tcatcattca
      721 acaatctgca aacatccagc acgcgttcat cactaaatga gccaagggtg ggtttgtttt
      781 tttctccatt tttcataatc tctatacgaa tcaaaagact tcacaaatgg ttgatatggc
      841 ttaacaggac acagcttcat gccctgcctg aaaaagcttt cacccaatgc caatcgctct
      901 tcaattgtga gataggaacc atcagctgga tccaaaagtt gttcacgcaa aagttttttc
      961 ttaaacccaa gaaaccgctg aaatgctcta taaaaaggcc aaagaggatc ccaccgatac
     1021 tctcgctttt ttgcaataaa tcgtgaacgt aaaataccac ccgactccag gcgcatcagt
     1081 tgtttctgca ccatgctcaa cgggatatca caacacttgg agatttcata agcactggcc
     1141 ctgcgttttt cataaaggtg aaaaaaaatc ttttcggctg ttttatttcc aaagagtgct
     1201 tccaacatat gcttatagta tagacaaacc ctatgaaaat ggcaagcaca atatacctgc
     1261 caaatatgta tatagaagac ctttaataat gcaccagcga cgtgatttca taacctttga
     1321 gaagatcacg acccttgaga aaatccagct ccaccaaaaa cacaaatccc acgatctcgg
     1381 cgcccacctg actgagcaac tgtgcgcacg ccttggccgt gccccctgtg gcgagcaaat
     1441 catccacgat caaaaccttt tcgcctttgg ccaccgcatc cacatgcatc tcgattttgt
     1501 ctgtgccgta ttccagcgtg tattcctgcg aaatttttgc ggcaggaagt ttgcccggtt
     1561 tgcggatggg cacaaacccg gcgttgagtg ccaacgcgag cggcgcgcca aagataaaac
     1621 cgcgcgattc tgccccgcaa accttggtga tgcccttacc cgcgtaaagt gccgtcattt
     1681 gttcgatggt cgccttaaac gcagtcgcgt ctttcaacag cgtcgtgatg tctttaaaca
     1741 agatcccttt tttgggaaaa tcgggaatgt cccgaatgat ttttgataga ttcatatctt
     1801 tgcgtgaatc gtgaatcgtg gatcgtaaat cgaaagaaaa aaacagtaac ttgtcattga
     1861 acgatccacg atccacgatt cacgattcac gtctttttcg gcaaatacaa caccggatca
     1921 accgcctcaa cccctttgtg aatttcaaaa tgcagatgtg gccccgaggc ccgtcctgtt
     1981 ctgcccactg agccaatctg ttgtccctgt ttgaccgttt tgcccacagt cacatttgtt
     2041 ttgtgacaat gcgcatacgc cgtaaaaaaa ttcttggcat gcttgatcac aatcaaattg
     2101 ccataaccag caaatttttt ctcaaaaatg atcttgccct ctgcggcggc cagaatgggc
     2161 gtgcctatct tcgcggacaa gtccaagcca tcgtgcggtc tgccgcgcct catgccaaac
     2221 ccactggtca ggtgaaattc cttcacaggc cagatcattt cgagatcacc gctatttctt
     2281 t
//
LOCUS       DATDUN010000754         1232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_530883, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000754 DATDUN010000000
VERSION     DATDUN010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530883"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VJC18_10560"
     CDS             complement(<1..700)
                     /locus_tag="VJC18_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45864.1"
                     /translation="MYQAIALILLAVALIIFMPKRHPVFYVSAGIVAGLSFFSSVASA
                     PIFPFFLAALVYHVMSGDVKNGLKAFLWVFLGGAAVVIWICSPDIPANLLVIFSQVEA
                     SKMRSHFNVSQPDVIQWVLRPDLTVRGGFRWVAGFGSTLLPVFSVVFCAAVMVFLLTG
                     VKAFLKRHWQWDVWVLLLLGLLPLLMTEYKQVAQLSRSYLPSFVGLIFVCALALRVFL
                     KEWWPGLNGSWRRLA"
BASE COUNT          422 a          364 c          241 g          205 t
ORIGIN      
        1 ccgccagcct gcgccaagat ccattcaaac ccggccacca ttctttaaga aaaactctca
       61 acgccagcgc acaaacgaat atcagcccaa caaacgaagg caaatacgaa cgggagagct
      121 gcgccacttg tttatactct gtcattaata acggcaacag cccaagaagc aataaaaccc
      181 agacatccca ttgccaatgg cgcttcaaaa aggccttcac ccccgtcaaa agaaaaacca
      241 tgaccgcagc acaaaaaaca acagaaaaca caggcaaaag agtggatccg aatcctgcca
      301 cccaccggaa gcctccacga accgtcaggt ctggacgcaa aacccattgg atcacatccg
      361 gctgactgac attaaaatga gaccgcatct tgctagcctc gacctgggaa aaaataacaa
      421 gaagatttgc gggaatatcc ggagaacaga tccaaatgac gaccgcagcc cctcccagga
      481 atacccataa aaaagccttt aatccgttct tcacatcccc tgacatcacg tgatacacca
      541 aggccgccaa aaagaacggg aagataggcg ccgaagcaac agacgaaaaa aaagaaagac
      601 cggcaacaat acccgcgctt acataaaaaa caggatgccg ctttggcatg aatatgatca
      661 acgccacggc cagcaggatc aaggcaatgg cctgatacac atggcacaag gtcaggacaa
      721 cttcgtacgg ccaataagag aacgcgaaca acagatatgc cagccatgcc acaggacttc
      781 cccacaaacg acgcagaata aaaaagatca ggagcccaat catggccagg cacatccctc
      841 caacaacaag aaacggccgc atggaatgga catcgcccat gacttgctgc gcccagaaat
      901 tcacaacaat aaaaaagtct tttcccctgg tggagaaatg attgggcgtt tcatacggca
      961 tgtgtaccgt tgacggagac cctccggccc ttaacaaatg tgtcgccggc tgaaaataat
     1021 tcaccgcatc cccaaagccc aagttcatcg accccgaagc tttccagaaa caaccgataa
     1081 aaaagaccgt cagacaggcc gtaacgacca aaacaaacag cacttccgga attctgaaac
     1141 aatgtctctc cgttttttca ctaacgatca tcaggttttc cctttcgatc cgctctcaac
     1201 aataaaaacc ttgtctttcc cccattgggc cg
//
LOCUS       DATDUN010000755         1094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_202274, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000755 DATDUN010000000
VERSION     DATDUN010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202274"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VJC18_10565"
     CDS             <1..190
                     /locus_tag="VJC18_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45865.1"
                     /translation="FKLDSKPPKMDYKEWALSENRFRMLTKSDPAAAEKLLNAANEEN
                     KFRWSFYEKIASLYEPKA"
     gene            201..953
                     /locus_tag="VJC18_10570"
     CDS             201..953
                     /locus_tag="VJC18_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLD45866.1"
                     /translation="MIDRAWSNLPPQVQDELIEYLEGFVSENRRRVLGQVLQQRTRHV
                     TVVLENIYQAHNASAIMRSCECFGVQDLHVIEKNYAFDPSAEVVMGAAKWLTVYKYRQ
                     QNTSLDVSLAQIKSRGYRLVATSLKPGAIPIQQLTVDQPVALCFGTEEVGLSDEVFQH
                     VDECVQIPMFGFTQSFNVSVSVALCLQEIVGRLHQSTGNWYLSDDEKRALLLEWMIRS
                     VRSGEQLVEKYLNAKQEACECRIMNKEQGISK"
BASE COUNT          278 a          244 c          299 g          273 t
ORIGIN      
        1 gttcaagctc gattccaagc cacccaagat ggattacaaa gagtgggctt tgtcggaaaa
       61 ccgtttccgc atgctcacca aatctgatcc ggcggctgct gaaaaactgc tcaacgctgc
      121 caacgaagaa aacaaattcc gttggagttt ttacgaaaaa atagcgagtc tgtacgaacc
      181 caaggcgtga gggtttcctc atgattgatc gggcgtggtc caatttgcct cctcaggtcc
      241 aagacgaact catcgagtat ctggaggggt ttgtcagtga gaaccggaga cgcgtattgg
      301 gtcaggttct gcaacaacgg acccgtcatg tgaccgtcgt tctcgaaaac atctatcaag
      361 cacacaatgc cagtgcgatc atgcgatcct gcgaatgttt tggggtgcag gaccttcacg
      421 tcatcgaaaa aaactatgcc ttcgatccca gcgcagaggt ggtgatgggg gcggccaagt
      481 ggttgacggt gtacaaatac cggcaacaaa acacatcact ggacgtgtcg ctggcacaga
      541 tcaagtccag gggttaccgg ctggtggcca cgtctctcaa gccgggtgct attccgatac
      601 agcaattgac ggtcgatcaa cctgtggccc tgtgttttgg gactgaagag gttggcttgt
      661 ccgacgaggt ttttcaacac gttgatgagt gcgtgcagat tcccatgttt gggtttacgc
      721 agagttttaa tgtgtcggtc agtgtggctt tgtgtctgca ggaaattgtg ggcagactcc
      781 atcaatcgac tggaaactgg tatctttccg atgatgaaaa acgcgcactc ttgctggagt
      841 ggatgatcag gagtgtgagg agtggggaac agcttgtcga aaaatatttg aatgcaaagc
      901 aggaagcgtg cgaatgtcga ataatgaaca aggaacaagg aatttcgaag taaaaactca
      961 tttcgacatt cgtcattcct tgctctctat tcgatattca ttttcatatg aaaatgcatc
     1021 gtctcatcct cgttggtccc aagtactcag gtaatgtggg agcatgcgcg cgcgtggccg
     1081 ccaattatgg tgtg
//
LOCUS       DATDUN010000756         1337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_571062, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000756 DATDUN010000000
VERSION     DATDUN010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571062"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VJC18_10575"
     CDS             complement(<1..527)
                     /locus_tag="VJC18_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLD45867.1"
                     /translation="MKREDFVFSILKPIDFGLELSLEALEKLNIKKQVSDASMDAMRK
                     LLFFQYTKMNDLKIEGKDSVPKTHGVIFACNHQSWADVQVLGSSCPRRIHFIAKGMFK
                     NWPVLRHLIDLNEGIYVTRTPKSKRQTKQELHNVIEALKEGKAVAIFPEGTIPGEEEI
                     PRHAVEPETGLLKGRT"
     gene            complement(698..>1337)
                     /locus_tag="VJC18_10580"
     CDS             complement(698..>1337)
                     /locus_tag="VJC18_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HLD45868.1"
                     /translation="DKTKELIRSEKNRLTLLWQKHEAGMSITLQPAAEQPEMVMVYPT
                     QKVLLDCLHKTPHGVFRMNPEIPDTVQTSNNLATLKVAQGSIFIGTLQRSSVATDLTQ
                     ISKDVADSFVPLGGQIEHFGAYPGWQLTTDSPLLSVASECAPRVYPRPAHVYVVHGGL
                     ECGLFKTVFPQLDQIAIGPEILGAHSPDERVNIASVGRFWDFLQEILKSIPS"
BASE COUNT          312 a          277 c          364 g          384 t
ORIGIN      
        1 gtcctgcctt tgaggagtcc tgtttctggt tcgacggcat ggcgggggat ttcttcttcg
       61 ccaggaatag tcccctcggg gaaaatggca acggccttgc cttctttgag ggcctcaatg
      121 acattgtgca attcctgttt agtctgtctt tttgattttg gggtgcgtgt aacgtagatg
      181 ccctcgttga ggtcaatgag gtggcggaga acaggccaat ttttgaacat gcctttggca
      241 atgaaatgga tccgacgtgg acaggatgag ccgagtacct gtacatcggc ccagctttgg
      301 tggttgcagg cgaagatcac gccatgtgtt ttggggacgc tatctttgcc ttcgatcttg
      361 aggtcgttca ttttggtgta ttgaaaaaaa aggagctttc tcatggcatc catgctggca
      421 tcggaaacct gttttttgat attcagtttt tcgagtgctt ccagagacaa ttccaaacca
      481 aaatcgatgg gttttaaaat ggaaaaaaca aaatcttcac gtttcatggg gtgcctccga
      541 gaacgaatga ataaagcaat gaaacaatga agaaatgttt ttacactaat tcatttcttt
      601 attgattcat tgattgatga tttcattgtt tcattttcct tgggttaata cgaaacaacg
      661 ctatgcgtca agagagtgct tgtttgaatt ttcggcatca actgggaatg cttttgagga
      721 tctcttgcag aaaatcccaa aaacgtccca cagaggcaat gttgacacgc tcatcgggtg
      781 aatgggcccc cagaatttca ggtccgatcg caatctggtc caactggggg aaaacagttt
      841 tgaaaaggcc gcattccagt ccgccgtgca ccacataaac gtgtgcaggc cgaggatata
      901 cgcgaggcgc acattcgctg gccacgctga gcagtggaga gtccgttgtg agttgccagc
      961 cggggtaagc cccaaaatgt tcaatctggc caccaagagg gacaaaggag tcggcgacgt
     1021 ctttgctgat ttgtgtgagg tctgtggcca cagagcttct ttgcagggtg ccgatgaaga
     1081 tactgccttg tgccactttg agtgtggcca gattgtttga tgtttgaaca gtgtctggga
     1141 tttcgggatt catgcggaaa acaccgtggg gcgttttgtg caggcagtcg agcaaaactt
     1201 tttgtgtggg gtaaaccatg accatttcgg gctgttcggc ggcgggttga agagtgatcg
     1261 acatgccggc ctcgtgtttt tgccacaaaa gcgtcagacg gtttttttct gaccgaatga
     1321 gttcttttgt tttatcg
//
LOCUS       DATDUN010000757         2554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_40031, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000757 DATDUN010000000
VERSION     DATDUN010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40031"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1239
                     /locus_tag="VJC18_10585"
     CDS             154..1239
                     /locus_tag="VJC18_10585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HLD45869.1"
                     /translation="MQFVDLKSQYLAYKTEIDSAIADVIQTTSFINGPAVEKLEKELS
                     DYIGVKHALTCSSGTDALLIAMMALGVKPADEIIVPAFSFIATASQIPLLGARPVFVD
                     VDPVTYNIDPVAISAKITSKTVGIIPVSLYGQCADYDAIQVIADKHHLWVMEDGAQSF
                     GATYKGKKSCFLTKISTTSFFPAKPLGCYGDGGAIFTSDDALADAIKKIKNHGQSKRY
                     HHDVIGVNGRMDTLQAAITSVKLKHFDQELELRNRVANLYSEHLHHTVDIPQVLPHNK
                     SSWAQYTIRTDQREELRTKLQTAGIPTAIHYPIPLNEQKAFTYLNQSVPLPVSGQLAR
                     EVLSLPMHAFITEAEIKLVCDVIKAKG"
     gene            1277..1633
                     /locus_tag="VJC18_10590"
     CDS             1277..1633
                     /locus_tag="VJC18_10590"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLD45870.1"
                     /translation="MSNFIDLKVWQRAKDLAVYVYQQTSCGFFAKDYGLRDQIRRAAV
                     SIASNIAEGDELDTDKQSIRHFYIAKGSSAETLTQAIISYEIGYLSEDVFKHIKDECQ
                     SISSMLAKLILARSKF"
     gene            1664..2161
                     /locus_tag="VJC18_10595"
     CDS             1664..2161
                     /locus_tag="VJC18_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HLD45871.1"
                     /translation="MSNYFVHESSYVDEGAEIGEGTKVWHFSHVMGGAKIGKNCVLGQ
                     NVNVGGKVTIGNNVKIQNNVSLYDGVMIEDGVFCGPSCVFTNVFNPRATVERKNEYRP
                     TLVKEGATIGANATIVCGVTLGRYCFVGAGSVVTKDVPDYALVYGNPARQCGKVDDLG
                     NKITS"
     gene            2169..>2554
                     /locus_tag="VJC18_10600"
     CDS             2169..>2554
                     /locus_tag="VJC18_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HLD45872.1"
                     /translation="MKKIVTIVGARPQFIKAAVVSRAIKEQPDHFSEIIVHTGQHYDP
                     NMSQVFFDEMQIPVPDYHLEIAGGNHGEMTGRMLIKIEEVLLKEKPDVVLVYGDTNST
                     LAGALAAVKLHIPVAHVEAGLRSFNR"
BASE COUNT          709 a          532 c          620 g          693 t
ORIGIN      
        1 acatgttccc cagcaaggag gatgtgctct cagggatcag gttgatggaa aaaatatcat
       61 attaacaata ggggcataag gttcaaggca taaggcttaa ggcagaagaa tttcacctta
      121 cgccctacgc cttgcgcctt gagccttgcg cttatgcaat tcgtagatct taaatcacaa
      181 taccttgctt acaaaaccga aattgattct gccattgccg atgtgattca aaccacatcg
      241 ttcatcaatg gtccggcagt ggaaaaattg gaaaaagaat tgtcggatta cattggcgtc
      301 aaacacgcac tcacatgcag ttctggcacg gacgcgctcc tcatagccat gatggctctg
      361 ggtgttaagc ccgcagatga aattatcgtc cctgcgtttt cattcatcgc cacggccagt
      421 cagattcctc ttttgggtgc caggcccgtt tttgtggatg tcgatcctgt tacatacaac
      481 atcgatcctg tggcaatcag tgcaaaaata acatccaaaa cagtaggcat tatccccgta
      541 tcactttacg ggcagtgtgc ggattacgat gccattcagg tcattgcaga caaacatcat
      601 ttgtgggtga tggaagacgg tgcgcaatct tttggtgcca catacaaagg taaaaaatcc
      661 tgttttctca ccaaaatttc tacaaccagt tttttcccgg ccaaaccatt gggttgttat
      721 ggtgatggtg gtgctatttt taccagtgat gacgcacttg ccgacgccat caaaaaaata
      781 aaaaatcatg gccagtcaaa aaggtaccat cacgatgtca ttggcgttaa tggccgtatg
      841 gatactttgc aggcggcgat cacgagcgtg aagctcaaac atttcgatca agaactggaa
      901 ctgagaaaca gggtggccaa tctgtattct gaacatttgc atcacaccgt ggatattccg
      961 caggtcttgc cccacaataa atcttcgtgg gcgcagtaca cgatccgtac ggaccaacgc
     1021 gaagaactga ggacaaaatt acagaccgcc ggtattccca cggcgattca ttatcccata
     1081 ccgctcaatg agcagaaggc ttttacgtat ttgaaccaat cagtgccttt gcctgtatcg
     1141 gggcaactag cgcgagaggt cttgagtctc cccatgcatg cgtttatcac ggaggcggaa
     1201 atcaagctcg tctgtgatgt gattaaggct aaaggctaaa ggcttaaggc caaaggcgaa
     1261 aggcaaattg attctcatga gtaattttat tgatttaaaa gtctggcaga gggcaaaaga
     1321 tttggcggtg tatgtttatc agcaaacaag ttgtggattt tttgctaaag attatggttt
     1381 gcgtgatcaa attcgtcgag cagcagtttc tattgcaagt aatattgctg agggtgatga
     1441 gctagataca gataaacaat caatacgaca tttttatatt gcaaagggtt cgtctgcgga
     1501 gaccctcaca caagcgatta tttcttatga gattggctat ttgtccgaag atgtattcaa
     1561 gcatattaaa gacgaatgcc agtctatatc atcaatgctt gcaaaattga tattggctag
     1621 gtccaaattt tagtttttag cctttagccc agaggttgat attatgtcca actattttgt
     1681 ccacgaatct tcctatgtcg acgaaggtgc cgagattggt gaaggcacca aggtttggca
     1741 tttttcgcac gtgatgggtg gtgccaagat tggcaagaat tgtgtattgg gtcaaaatgt
     1801 gaatgtgggt ggcaaagtta ccattggcaa caacgttaaa atccaaaaca atgtatcgct
     1861 ttacgatggt gtgatgatcg aagatggggt gttttgcggg ccctcttgtg tttttacaaa
     1921 tgtttttaat ccccgagcga cagttgaacg aaaaaatgag tacagaccca cgctggtcaa
     1981 agaaggtgcc acgatcggcg ccaatgccac gattgtttgt ggagtcactc ttggccgata
     2041 ctgttttgtg ggtgcagggt ccgtggtgac aaaagatgtg ccggattatg cgcttgttta
     2101 cggcaatccg gccagacagt gcggtaaagt tgacgatttg ggaaataaaa tcacttctta
     2161 aaaaaaacat gaaaaaaatc gtgactattg tcggtgccag gccccaattc ataaaggcgg
     2221 ccgtggtttc gcgggcgatc aaggagcaac cagatcattt cagcgaaatc atcgttcata
     2281 ccggtcagca ttatgatccc aacatgtctc aggtgttttt tgatgagatg cagattccgg
     2341 ttcccgatta tcatcttgag atagcaggcg ggaatcacgg ggaaatgacg ggccgcatgt
     2401 taatcaagat cgaagaagtt ctcctcaaag aaaaacccga tgtggtgctt gtttatggcg
     2461 atacgaattc tactttggca ggggctctgg cggcggtcaa gttgcacatt cctgttgccc
     2521 atgttgaagc cggtttaaga agttttaatc gaag
//
LOCUS       DATDUN010000758         1210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_158576, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000758 DATDUN010000000
VERSION     DATDUN010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158576"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VJC18_10605"
     CDS             <1..460
                     /locus_tag="VJC18_10605"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLD45873.1"
                     /translation="FMLKLNVTYPSVEEELAIMNRFARKDQGVAQKVLSPQVVLAMRE
                     IVERIYIDPKINDYIVRLVFATRDPKKLGKGLERQIQFGGSPRASINLHRAARCWAFL
                     QGRAYVVPQDVKTMAPDVLRHRILLTYEAEAEGLTTDNIIQTILDNVEVP"
     gene            596..>1210
                     /locus_tag="VJC18_10610"
     CDS             596..>1210
                     /locus_tag="VJC18_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLD45874.1"
                     /translation="MLSAELIKKIDSLFIRSRHRVTDVFSGEYESAFRGRGIEFEEFR
                     EYVPGDDIRQIDWHVTARMDKPFVKIYREEREQTIFFLVDSSRSLKFGQKREKLEVAT
                     ELAALLSYAAIKSHDKVGLIIFSDQIEKYIAPKQGRAHVWNIIATLLTHKPRGTKTDI
                     NQATQFFLNVTRRKTVCFLISDFLDVDCRDSLRVASFKHEFVVMR"
BASE COUNT          305 a          300 c          321 g          284 t
ORIGIN      
        1 gttcatgctc aaactcaatg tcacgtaccc gtctgttgag gaagaacttg cgatcatgaa
       61 tcgatttgcc cgcaaggacc aaggggttgc gcaaaaggta ttgtccccgc aggttgtgtt
      121 ggccatgagg gagattgtcg aaagaatcta catcgatccc aaaatcaacg actacatcgt
      181 gcggctggtg tttgccacgc gtgaccccaa aaaattgggc aagggtctgg agcgccagat
      241 ccagtttggt ggctctcccc gggccagcat caatctgcat cgcgccgccc gttgttgggc
      301 ctttttgcag ggcagggcgt atgtggttcc tcaggatgtc aagacgatgg ccccggatgt
      361 gctcaggcac cgcatcctgc tcacttacga ggcagaggca gagggattga ccacggataa
      421 catcattcag acgattttgg ataatgtgga ggtgccgtaa atggtaatgg gtaatgggta
      481 atgggtaatg ggtgaaggaa caataaatat cgaacaggga atgtcgaagt gacattttgt
      541 ccttacactc aagggcacaa gcgatccacg atccacgatc gtcgatccac gagttatgct
      601 atccgcagaa ctgatcaaaa aaatcgattc cctttttatc cgttcgcgtc accgcgtcac
      661 cgacgtgttc agcggtgagt acgaaagtgc cttccgcggt cgtgggatag agttcgagga
      721 gtttcgcgaa tacgttccgg gcgatgacat tcgtcagatc gactggcacg tgaccgcccg
      781 catggacaaa ccttttgtca aaatttatcg cgaggagcgc gaacagacca ttttttttct
      841 tgtggattct tcgcgcagtc tcaaattcgg tcaaaaaaga gaaaagctcg aggtggccac
      901 ggaactggcg gcgctcttgt cttacgcggc gatcaaaagt cacgacaagg tgggactcat
      961 catcttttcg gaccaaatcg aaaaatacat tgcccccaaa caagggcgcg cgcacgtctg
     1021 gaacatcatt gccacactgc tcacgcacaa acctcgggga accaaaacag acatcaatca
     1081 ggccactcaa ttttttctca acgtaacaag gcgcaagacg gtgtgttttc tcatttccga
     1141 ttttctagac gtggattgcc gcgactcgtt gcgcgtggcc agcttcaaac acgaatttgt
     1201 tgtgatgcgt
//
LOCUS       DATDUN010000759         1307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_531097, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000759 DATDUN010000000
VERSION     DATDUN010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531097"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VJC18_10615"
     CDS             <1..199
                     /locus_tag="VJC18_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45875.1"
                     /translation="DINPAKTDEILQLAEANGLTCVGNIHYGNAVTEAQCAGQTVIEY
                     GEPCMAGDFGNVWKNTIALLK"
     gene            complement(515..1096)
                     /locus_tag="VJC18_10620"
     CDS             complement(515..1096)
                     /locus_tag="VJC18_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLD45876.1"
                     /translation="MKIDLSKIKHIVLSHNHWDHVDGLWGLLKHHYGIRVYLCPKFGK
                     PLSDRIRRAGAIPVTVQGWKAMEHGIYLTAQMIGRYKDKPIAERSLVISSGETLVIIT
                     GCAHAGAEAIIKQIKAKFPGKRIAALMGGLHLKDMSQIQIRRTVTSLQASGIQKLMAC
                     HCTGAMGLKAVQKSYGRNSQMIKAGSKIALNLI"
     gene            complement(1104..>1307)
                     /locus_tag="VJC18_10625"
     CDS             complement(1104..>1307)
                     /locus_tag="VJC18_10625"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HLD45877.1"
                     /translation="FGTGGGKRLAEETGLPFLGSIPLAPAFVVQGDLGKPMMTTTFED
                     VKRPLMAIVQAIINPTNRHKNKA"
BASE COUNT          326 a          295 c          329 g          357 t
ORIGIN      
        1 ggatatcaat ccggccaaga cagatgaaat tctacaactg gctgaggcaa atgggttgac
       61 ctgcgtcggc aacattcatt acggcaatgc ggtcaccgag gcgcagtgtg ccggtcagac
      121 ggttatcgag tatggcgagc catgcatggc cggagatttt ggaaatgttt ggaagaatac
      181 cattgccttg ttaaaataat tcaatttcaa cctgaacagt caggagtgtt ttatgtcggc
      241 acaatgtgaa aatttggcga cctgcggtct ttttcgggaa tatcagggca atagtgaggt
      301 gataaaaaac gggtggattc ggatgtattg caatgatatc ctgacatctg aaaaatgtca
      361 gcgtaaacag attaaaaagt aaaaagtcaa ccggcaagcc gcccgtatca aacatggcac
      421 ccactgggaa gatgaccatt atttcaacaa aacaagacaa aacattcttg atggttcccg
      481 cggttctatc ttgttcttta cttaagaccg caaatcatat caagttaagg gcaattttac
      541 tgcctgcttt tatcatttga ctgttacggc cataggactt ttgtacggcc ttgagtccca
      601 ttgcgccggt gcaatgacag gccatcagct tttgtatccc gctggcctgt agagatgtca
      661 cggttctccg gatttggatt tggctcatat ctttcaggtg aaggccgccc atcaatgcgg
      721 cgattctttt tccgggaaat tttgctttta tctgtttgat gattgcttct gcgccggcat
      781 gtgcgcaacc ggtgatgata acgagagtct caccggacga gatcaccagt gagcgttctg
      841 caatgggctt gtccttgtat cgaccgatca tctgtgcggt cagataaatg ccatgttcca
      901 tcgctttcca accttgaacg gtaaccggga tggcaccggc gcggcgtatt ctgtcggata
      961 gcggttttcc gaattttggg cacagataaa cccggatgcc ataatggtgt ttcagcagac
     1021 cccataatcc atcaacatga tcccaatgat tgtgagatag gacaatatgt ttgattttgc
     1081 taaggtcgat tttcatggat gatttaggct ttgtttttgt gacggtttgt agggttgatg
     1141 atggcctgca caattgccat cagaggacgc ttcacgtctt cgaatgtggt agtcatcatg
     1201 ggttttccca agtcgccctg caccacgaat gcgggagcaa gtgggatact gcccaaaaac
     1261 ggcaggccgg tctcctcggc caaccgcttg ccacccccag tgccgaa
//
LOCUS       DATDUN010000760         1092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_116348, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000760 DATDUN010000000
VERSION     DATDUN010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116348"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VJC18_10630"
     CDS             <1..339
                     /locus_tag="VJC18_10630"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HLD45878.1"
                     /translation="SPRNPQYHAEYSGSPEQRDSAYHQGTVWPWLLGHFVEASLKCAT
                     DPQQTAEVLLKRLSPLWHSHLRVACVGGVSEIFNGDPPHTQKGAMHQAWSVAEVIRAI
                     TLLENVGNSL"
     gene            336..>1092
                     /locus_tag="VJC18_10635"
     CDS             336..>1092
                     /locus_tag="VJC18_10635"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLD45879.1"
                     /translation="MKVLMFGWEFPPYISGGLGTVCKAMTEHLARLGVKVCFVMPRVI
                     GDKPQVPNFELLGANELTFRQTIQSVYKKLSQETIQFLEVDAFLNPYFNEETYQEKMG
                     SYLKERIDLVREMEKQGVHRMDFHGGYGRDLLSEVSRYALVGSLLGMQHDFDLIHAHD
                     WICYPAGVEAKRASGKPLICHVHATEFDRTGGNPNQEIYNIERYGLENADRIFTVSQR
                     TKDTVSFHYGIDPEKIHVVYNAVSKEKLVERHQI"
BASE COUNT          250 a          255 c          309 g          278 t
ORIGIN      
        1 tctcctcgta atccgcaata tcacgcggaa tattcaggat ctccagaaca gagggattct
       61 gcttatcatc aggggactgt ctggccctgg ttgctggggc atttcgtgga agcttccctc
      121 aagtgcgcga ctgatcccca acagacggcg gaggtcctgc tcaaaagatt atctcctttg
      181 tggcacagtc atttgcgtgt ggcctgtgtt ggtggtgtgt ccgagatttt taatggcgat
      241 cctccccaca cacagaaggg cgccatgcat caggcttgga gcgtggctga agtgattcgt
      301 gccatcacct tgttagaaaa cgttggtaac agcttatgaa agtgttgatg tttggctggg
      361 agtttcctcc ctatatcagt ggtgggttgg gtacggtctg caaggcgatg actgaacact
      421 tggccaggct gggtgtgaag gtgtgttttg tgatgcctcg cgtgattggc gataagcctc
      481 aagttcccaa ttttgaactg ctgggtgcca acgaactcac gtttcgccag acgatccaga
      541 gcgtgtataa aaaattgtcg caggagacca ttcaatttct ggaagtcgat gcttttttga
      601 atccttattt caacgaagag acctatcagg agaaaatggg gtcttacctc aaggaacgga
      661 ttgatctcgt ccgcgagatg gaaaaacagg gcgtgcaccg catggatttc cacggtggtt
      721 atggtcgtga tctgttgtcc gaggtctctc ggtacgcgtt ggtggggtca cttttgggca
      781 tgcaacacga tttcgatctg atccacgcgc atgactggat ctgttacccc gcgggagtcg
      841 aggccaagcg ggccagcggc aagcccctca tttgtcacgt gcacgcgacg gagttcgatc
      901 gcacgggtgg gaatcccaat caggaaattt acaacatcga gcgctatggt ctggagaacg
      961 cggatcgcat tttcacagtt agccagcgca cgaaggacac tgtgtctttt cattatggca
     1021 tcgatcccga aaaaattcat gtggtgtaca acgccgtgag caaggaaaaa ctggttgaac
     1081 gacaccagat ca
//
LOCUS       DATDUN010000761         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_531137, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000761 DATDUN010000000
VERSION     DATDUN010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531137"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VJC18_10640"
     CDS             complement(<1..790)
                     /locus_tag="VJC18_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLD45880.1"
                     /translation="MLIEIITIGDELLSGNVLDTNTQFLSDHLFRHGFMVEYHTGLRD
                     DETKMVTAFETASDRADIVLVTGGLGPTADDFTIEMAAKTFGKKLVCDQSVLHYLETW
                     YAGHHIPLQDNTRKQAHVPEGALTFINKEGTAPGVYCHYKKSHFYFMPGVPREMRWLF
                     TTHILPHILSLHPLKQSVATRFFKTFGSPESALDAELNTLFSDRVTLGGVRVGYRVAF
                     PEITLKLTAYGQGELEAQDKLDEASQIIRSKFASVIYSEDEEGTL"
     gene            973..>3016
                     /locus_tag="VJC18_10645"
     CDS             973..>3016
                     /locus_tag="VJC18_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45881.1"
                     /translation="MFDTAINPEQIDVSAEVLEDRYCLSQSPQTDALQSLRAGLSHSR
                     FFMSASEISAELRERAKRVFPPQSRQSRPGSVGVRLAAEHFGVTSRVMLHAFSGDRKD
                     FYTPNQKRRSPKAQSYDGVLGQRMARFFKTRFNYLSFEGPPLQDPAIIWNEIVFRCDE
                     LQLGRDELCPLLGVPLSYSAFKELILTRGPLPDNIRIAMTELLHIRLWPHFEKIPFES
                     LEGLATRINQTLAEWRMSHLELSILVGRGIMTDDISRVVGGHVQRTTLPALVLAEYFG
                     FEIREQVSHEIPEVGLVSTLYELDQELMAQIRACLEASGKTLAMVAQETGLYCVDGLQ
                     VMIDGEREITAYQQFTLLRRSLEPLCDYLGIRLRRFEDMTPTDVWATEEDRERALKET
                     AVVQACLDVMNQANRRFLFSQNEVMARYNQRFGPPLMTAKVLNRLMSGDFTFKKAGLY
                     WHNLLSLYKINVRQGVLFAASQSSEINYDALWRVVQARTGTDLLSTHPLSTRYDAYRY
                     REKTLPLADPRFLQRCVMAGAHGHVSVQILLEQGNQEARAIALNLPESFVLFFPSTKE
                     GWAEVVERRQTQLRQQLANYLKTKQAEWRDKLKEDRLQKGRPKQTKVKVGFYFQYRDL
                     TNTVFEHLFDDVHSAVEASDESFIQPTGGDLSVLAGAVPDHLCYLFSVGDVFSVAWA"
BASE COUNT          714 a          684 c          819 g          799 t
ORIGIN      
        1 cgagggttcc ctcctcatct tctgaatata tgacagacgc aaattttgaa cgtatgatct
       61 gtgacgcctc gtccagcttg tcttgtgctt caagttcgcc ttgcccgtag gctgtgagtt
      121 tgagggtgat ttcgggaaaa gcaacacggt accctacgcg cacgccacca agagtcaccc
      181 gatcagaaaa taatgtgttc aattcggcat ccagggccga ttcggggctt ccaaaggttt
      241 tgaaaaaacg cgtggccaca gattgcttga ggggatgcag actcaaaatg tgtggcagaa
      301 tgtgcgttgt gaacagccaa cgcatttctc gtggcacgcc aggcataaag taaaaatgac
      361 tttttttgta atgacagtaa actccggggg ctgtgccctc tttgttgatg aaggttaagg
      421 ctccttcggg aacatgagcc tgtttgcggg tgttgtcttg cagagggata tgatgccctg
      481 cgtaccaagt ttcgagataa tgcaataccg attggtcaca gacaagtttt ttaccaaagg
      541 tcttggccgc catttcgatg gtaaagtcat cagctgtcgg gcccagccct ccggtgacca
      601 aaacaatatc ggcgcggtcg ctggcggttt caaaagccgt gaccattttg gtttcatcgt
      661 cgcgtaagcc cgtatgatat tccaccataa agccgtgacg aaataaatga tccgataaaa
      721 attgagtgtt ggtgtcgagc acattgcctg agaggagttc gtcccctatg gtgatgattt
      781 cgatgagcat gtgagaacct tagaactgtt tataccgtgt gtcaccgctt ttttgtacgg
      841 gcttgacgcc ggtgcgagga gtgtcatagc actccccaag cgcaaccaag gcaaaacaac
      901 atggccgggt caatttttac ttcaaataca agtctcatcc ctcttcaata tcttgcacag
      961 ggcgcaaggg ttttgtttga tacggcaatc aatccagaac agatcgatgt ttctgctgag
     1021 gtcttggagg atcggtattg tttgtctcaa agcccccaaa cggatgcctt acagagtctt
     1081 cgggctgggc tgtctcacag ccgatttttc atgtctgcgt cagagatcag tgcagagctt
     1141 cgtgagcggg caaaacgggt ttttccacca caaagtcgcc aatctcgccc cggtagtgtt
     1201 ggggttcgct tggccgcaga acattttggt gtgacctcgc gggtcatgtt gcatgcgttt
     1261 tcgggggacc gaaaagattt ttacacaccc aatcaaaaac gcagaagccc aaaggcgcaa
     1321 agctatgatg gggtgctggg ccaaaggatg gcgcggtttt tcaaaacccg atttaactat
     1381 ttgtctttcg aaggcccccc tttgcaagac cccgcgatca tctggaatga aattgtgttt
     1441 cgatgtgatg aacttcagct tgggcgtgac gaactttgtc ctttgttggg ggtgccgttg
     1501 tcgtattctg cctttaagga gctgattcta accagaggcc cgttgcccga caatatccgt
     1561 attgccatga cggaactcct gcacatacgc ctctggcccc attttgaaaa aattcctttt
     1621 gaatctttgg agggactggc gacacgcatc aaccagacgc tagccgagtg gcgcatgtcg
     1681 catttggagc tgtccatatt ggtaggccgc gggatcatga cggatgacat ttcacgagtg
     1741 gttggtgggc acgtacaacg aaccacattg cccgctttgg tgttggctga gtattttgga
     1801 tttgagatta gggaacaggt gtcccacgag attcctgagg tgggactggt ttcaacgctg
     1861 tatgagcttg atcaggagct catggcccag atccgcgcct gtctcgaggc aagcggtaaa
     1921 acactggcaa tggtcgccca agaaaccggc ctttattgtg ttgatgggct tcaggtcatg
     1981 attgatggtg aaagagagat caccgcgtat caacaattca cgctgttgag acgctcactg
     2041 gaacctttgt gtgattatct ggggattcgt ttgagacgct ttgaggacat gacgcctaca
     2101 gatgtgtggg ccacagaaga ggacagagag agagctttga aggaaacagc tgttgtgcag
     2161 gcttgtttgg atgtgatgaa tcaagccaac aggaggtttt tgttcagcca aaacgaagtc
     2221 atggcgcgct ataatcagcg atttgggcct cccctgatga cggcaaaagt tctcaaccgc
     2281 ctcatgagtg gcgattttac attcaagaaa gccgggttgt attggcacaa ccttttgtcg
     2341 ctatataaaa tcaacgtgag acagggggtg cttttcgcag catcgcagtc gtcagaaatt
     2401 aattacgatg ctttgtggcg ggtggtccag gcccgcacag gaacggattt gctgtcaacc
     2461 caccctctgt caacgcgtta tgatgcttat cgatatcgag aaaagaccct gccgctggcc
     2521 gatccccgtt ttttacagag gtgtgtgatg gctggcgcgc atggtcacgt ttcggtgcaa
     2581 atattactgg agcagggcaa tcaggaggcc cgtgccattg cccttaattt gccagaaagt
     2641 tttgttttgt tttttccctc gaccaaagaa gggtgggccg aggttgtgga acggcggcag
     2701 acacagttaa ggcaacagct ggcaaattat ctcaaaacaa aacaagccga atggcgtgat
     2761 aaacttaagg aagataggct tcaaaagggt cgaccgaaac aaaccaaggt caaggtgggt
     2821 ttttattttc agtaccggga tttgacgaat acggtatttg agcatctttt tgatgatgtt
     2881 cattccgctg ttgaggccag tgacgagtct tttatacagc ccacgggagg cgacctctcc
     2941 gttttggccg gtgcggttcc agatcatctt tgttatttgt tttcggtagg cgatgtgttt
     3001 tcggtggctt gggcct
//
LOCUS       DATDUN010000762         3013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_240172, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000762 DATDUN010000000
VERSION     DATDUN010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240172"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VJC18_10650"
     CDS             <1..647
                     /locus_tag="VJC18_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45882.1"
                     /translation="KDETTVDKINDTLDGVSQFVGGANRTRLDLGMHTEYLAGTGNFK
                     NYVSMSIKPRPDKYFLFEVVSDPDPSFNTSIEETTVSSGGNTSVVTTRSRSKELDGLL
                     FSFQIAKRYRDFSIRGGLIESSGGVGIDYDNGPVGLSFTAFDFKSEEGEKPHLKALAR
                     TNLTKSFYLLAGVDDIINPAQDLAWYLGAGLTFTDDDIKSLLGLVSSVGTSSVK"
     gene            652..1887
                     /locus_tag="VJC18_10655"
     CDS             652..1887
                     /locus_tag="VJC18_10655"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HLD45883.1"
                     /translation="MATINSHYLTLASGYLFPEIGRRVAQYREQNPQARIIRLGIGDV
                     TRPLPQVVVDAAKAAFDEMALASSFKGYGPEQGYTFLREAIAVNEYASRGVAITPDEI
                     FVSDGAKCDTGNIQELFDARCRIAITDPVYPVYVDSQVMAGRAGERDQAGRYDQLVYL
                     PALPENGFLPELPAKKIDVVYLCFPNNPTGVVAPLSYFKMWVDFALQNDVIILFDAAY
                     EAFITDQTIPHSIFEIEGAKNCAIEFRSFSKKAGFTGTRCAFTVVPKDLMGRDGEGHE
                     VPIHPLWLRRQTTKFNGVSYPVQRAAEACYTPEGQRQIQETINYYLENARLIRQGLLK
                     CGFSVSGGVNAPYLWFKAPVASSWDYFNHLLHTANVVTTPGAGFGACGEGFMRLSAFG
                     DRADVEEAMERISGIPASG"
     gene            complement(2034..>3013)
                     /gene="ligA"
                     /locus_tag="VJC18_10660"
     CDS             complement(2034..>3013)
                     /gene="ligA"
                     /locus_tag="VJC18_10660"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HLD45884.1"
                     /translation="PVDIGGVTVSRATLHNFDYVNQLDVRVGDWVRVARAGDVIPAII
                     SVATDKRSGALSKIIPPTHCPVCSAPVVQNKSFFYCTNPLNCPAQMKWALVHFGSKRA
                     LNIAGLGEETVALLMQQQLVQNCADLFTLTQDTLMTLEGFQEKKSQNLIQAIRDCTHK
                     PGDKQLFALGIRDVGEQTAKLIMQHFGSLDRLMRATDDDLLSIDGIGPETVASIRQFF
                     GHPSNLAMISRLVQAGMFRTTLAVVSAQKGPLHGQTFVLTGELESFTRDELKEKLESL
                     GAKVTGSVSQKTNYVVVGAHPGSKLDKARELGVTILGEAQVIALMRDNK"
BASE COUNT          692 a          743 c          808 g          770 t
ORIGIN      
        1 tcaaggacga aacgactgtc gacaagatca acgacaccct agatggtgtc agtcaattcg
       61 tgggaggggc aaaccgcacg aggctcgatt tgggaatgca cactgagtac ctcgcgggta
      121 ccggtaattt taaaaactat gtgtcgatgt ccatcaaacc gaggcccgac aaatatttcc
      181 tctttgaggt cgtgagcgat cccgatccca gctttaacac cagcatcgag gagaccacgg
      241 tgtcatccgg tggcaatacc agtgtggtga cgacgcgcag ccgttccaag gaactcgacg
      301 gtttgttgtt ctccttccag attgcaaaac gatatcgcga tttctccatc aggggtggtt
      361 taattgaatc ttcaggcggt gttggtatcg attacgataa tggaccagtg gggttgtcct
      421 tcacggcgtt tgatttcaag agtgaggagg gtgagaagcc tcacctcaag gccttggcgc
      481 gcacgaacct caccaagagt ttttatcttt tggcaggcgt tgacgacatc atcaacccgg
      541 cgcaggatct ggcctggtac ctgggggcag ggttgacctt caccgacgac gacatcaaat
      601 ctctgctggg actggtttct tctgtcggca cgtcttctgt taagtgattt catggcaaca
      661 ataaattcac actatctcac acttgcttcg ggctacttgt ttcccgagat tggacgtcgt
      721 gttgcacaat acagggagca gaacccgcag gccagaatca tccgcttggg tattggtgat
      781 gtcactcgac ccctgcctca agtggtggtg gatgccgcca aggcggcctt cgacgaaatg
      841 gccctggcct catccttcaa agggtatggt cccgagcaag gttacacttt tttacgcgag
      901 gcaattgccg tgaacgaata cgcgtcacgt ggtgtggcga tcaccccaga cgaaattttt
      961 gtttctgatg gcgccaagtg cgatacaggc aacattcagg aattgttcga cgcgcgttgc
     1021 cgaattgcca tcactgatcc cgtgtaccct gtctatgtcg attcccaggt catggcggga
     1081 cgtgcgggag agcgtgatca ggcagggcgt tatgatcaat tggtttattt accggcactg
     1141 ccggaaaacg ggtttttacc ggagctaccc gcaaaaaaaa tcgatgtggt gtatttgtgt
     1201 tttcccaaca atccaactgg tgtggtggcc cccctgtctt atttcaagat gtgggtggat
     1261 tttgccctgc agaacgatgt gattattctt ttcgatgcgg cttatgaggc cttcatcacc
     1321 gaccagacaa tccctcattc cattttcgag atagagggtg ccaaaaattg tgccatcgag
     1381 tttcggtcgt tctccaaaaa ggcggggttc acgggcactc gttgtgcctt cactgtcgtg
     1441 ccgaaggatc ttatgggtcg tgatggtgag ggacacgagg ttccaattca cccgttgtgg
     1501 ctcagacgac aaaccaccaa atttaacggt gtgagttatc ccgtacagag ggccgctgag
     1561 gcctgttaca cgccagaggg acaaagacag attcaagaaa cgataaacta ttatctcgaa
     1621 aacgcgcgcc tcatccgtca gggactactc aaatgtggtt tctctgtttc tgggggcgtc
     1681 aacgcgcctt atctgtggtt caaggccccc gtagcgtcat cctgggatta tttcaatcat
     1741 ctcctgcaca cagcaaatgt ggtgacgacc ccaggtgccg gttttggggc ttgcggcgaa
     1801 ggattcatgc ggctttccgc ctttggtgat cgcgcggatg tggaagaggc gatggaacga
     1861 atttccggca tcccagcatc cggttaatct ggttcagtcc tacggtcgat atgctgtgat
     1921 gccgttcgaa tatcgaatga tgaacaagga ataacgaatg tcgaaggcga ttcattttat
     1981 ttcgacattc cttgttcgat acctgtcctg aacgaagccg aaggattcat tattcatttg
     2041 ttgtctcgca tcagcgcaat cacctgagct tcacccagaa tagtcacacc caattccctc
     2101 gctttgtcta atttgcttcc cggatgtgcg cccaccacaa cataattcgt tttttgactc
     2161 acactgcctg tgaccttggc ccccaaggat tcaagcttct ctttgagttc gtccctcgtg
     2221 aacgattcaa gttcacccgt gaggacaaag gtttggccgt gcaaaggtcc tttttgagca
     2281 gacacaacgg cgagtgtcgt gcgaaacatg ccggcctgca caagacgtga aatcatggcg
     2341 aggttagatg ggtgaccgaa aaattgtctg atggaggcga ctgtctctgg tccaatgcca
     2401 tcgatagaga gcaggtcatc gtctgtggcc cgcatgagac ggtccaaaga tccaaagtgt
     2461 tgcatgatga gttttgctgt ttgttccccc acatcgcgga tccccaaagc gaagagttgt
     2521 ttgtcgcctg gtttgtgggt acaatcccga atggcttgga tgagattttg cgatttcttt
     2581 tcctgaaaac cttccaaagt catgagcgtg tcctgagtga gggtaaagag gtcggcacag
     2641 ttttgaacaa gttgttgttg catcaagagg gccactgttt cttcacccaa accggcgatg
     2701 ttgagggccc gttttgatcc aaagtgaacg agcgcccatt tcatttgtgc ggggcaattg
     2761 agagggttgg tgcaatagaa aaaagatttg ttttgcacca caggggcact gcaaacaggg
     2821 caatgggtgg gagggatgat tttggacaag gctcccgatc ttttgtcggt ggcaaccgag
     2881 atgatggcag ggatcacatc ccctgcacgg gccaccctga cccaatcacc aacgcgcaca
     2941 tcgagttgat tgacgtagtc aaaattatgc agtgtggccc ggctgactgt cacgccgcca
     3001 atatcgacgg gtc
//
LOCUS       DATDUN010000763         1744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_497033, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000763 DATDUN010000000
VERSION     DATDUN010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497033"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VJC18_10665"
     CDS             <1..414
                     /locus_tag="VJC18_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45885.1"
                     /translation="DQGLPWQPLVPPEVKLLERTVFREARDLSEQLLSVFGRVDALDP
                     TTIMATNADDPIVGRHDMNIHFQALGLKVSAHVLCGAGVSSNVTLRYSANLPSTPSAE
                     TAPQQNYRIMFSFSDAAHMARTPAFVQELCLTSHD"
     gene            703..1500
                     /locus_tag="VJC18_10670"
     CDS             703..1500
                     /locus_tag="VJC18_10670"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLD45886.1"
                     /translation="MLSISQLPHPVRETVNERGLSPHDALQAFLQFLVIKHHSFEPAR
                     FIGGTALVLGHGNPRFSEDIDFTAVTDPLKLKPGLQRAVTEIEQWLGVRVTLTDPKPG
                     KATFRIQCHYDRATTVSLHIDSQSYVSQTHKPIVAKYATLAPVICSSVTVWEIMADKL
                     LAVAFRNYLAGRDVFDLWYHWLRLDSCEKDCELVVPLFKQKCKQRHIKLEHWRERLQS
                     QVNYQKQEKRMTQEWQRFLPQEFQKLMIQQDMWQAFIRLTDLLREHI"
     gene            1497..>1744
                     /locus_tag="VJC18_10675"
     CDS             1497..>1744
                     /locus_tag="VJC18_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45887.1"
                     /translation="MTQVELIKALNALSLQHKKKIFTLKELAIWFGHSRASVAMGLIR
                     AKRQSLVFSVNNFWFNQLDPPTLEQLALTIKSPSYISF"
BASE COUNT          447 a          451 c          391 g          455 t
ORIGIN      
        1 gatcaaggtc tgccgtggca acctttggtg cctccagaag tgaagctgtt ggaacgcacc
       61 gttttccgtg aagcgcgcga tctatccgaa cagcttttgt cagtcttcgg aagagtggat
      121 gccctggatc ccacaacgat catggcaaca aacgctgatg atcccattgt ggggcggcat
      181 gacatgaaca ttcactttca ggccttgggt ctaaaagtga gtgcccatgt tttgtgcggc
      241 gcaggagttt ccagcaacgt gacactgcgt tattctgcca acctgccatc aacaccctcc
      301 gcagaaacgg ctccacaaca aaactacagg atcatgtttt ctttttctga tgcggcacac
      361 atggctcgca ctccagcgtt cgtgcaggaa ttgtgtctca cttcacatga ctgaatctgt
      421 ttcgttatat tttaaaaaat aatcgcgact attgactcaa cccagcctgg gtttatcgaa
      481 aatacctgct caaccgatcc tctccgtctt gagccagata atgtgggtgg cactttttcg
      541 tccgattgtt gcttgccccg tggcgaagct ttaaggggct tgccccgtgg cgaaacttta
      601 cggggcttgc cccgtggcga aactttacgg ggactggcac cttacgacag tcgccacttt
      661 ttactcattg catgatatta accaatatgt taatatcgta ccatgctatc aatctcccaa
      721 ttaccccacc ccgtcagaga gactgtgaac gagcgtggtc tttcgcccca cgatgccttg
      781 caggcctttc tgcaatttct ggtcatcaag catcattcct ttgaacccgc acgttttatc
      841 ggtggaaccg ccttggtgtt gggtcatggc aacccgcgtt tttcggaaga tattgacttt
      901 acagccgtta ctgatcccct caaactcaag cccggtctgc aaagggccgt cacagaaatt
      961 gagcaatggc tgggtgtcag agtcacgttg accgatccca aacccggtaa agccacgttc
     1021 agaatacaat gtcattatga ccgggcaacc actgtcagtc tacatatcga ttctcagtcc
     1081 tatgtatccc agacccacaa acccattgtg gcaaagtacg cgaccttggc accggtcatt
     1141 tgttcctctg tcacagtctg ggaaattatg gccgacaagc ttctggctgt ggccttcaga
     1201 aactatctgg cgggacgaga tgtctttgat ctctggtatc actggttgag acttgattcc
     1261 tgcgaaaagg actgtgaact tgtcgttcct ctgttcaaac aaaaatgcaa acaacgtcat
     1321 attaagctgg aacattggcg tgaacgctta cagtcacaag tgaattatca aaaacaggaa
     1381 aaaaggatga cacaagaatg gcagagattt ttgccacagg aatttcagaa actaatgatc
     1441 caacaggata tgtggcaggc gtttatcaga ctgacagatc tattgagaga acacatatga
     1501 cccaagtcga actcataaag gctctcaatg ctttgtcgct acaacataaa aagaaaatct
     1561 tcacgttgaa ggaactggca atctggttcg gacattcacg tgcctcggtc gccatgggac
     1621 tcatccgcgc aaaacggcag agtttggttt ttagcgtcaa taacttctgg ttcaaccaac
     1681 tcgatccgcc cacccttgag caattggcgc ttacgataaa atcaccctca tacatctctt
     1741 ttga
//
LOCUS       DATDUN010000764         3144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_372491, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000764 DATDUN010000000
VERSION     DATDUN010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372491"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..986)
                     /locus_tag="VJC18_10680"
     CDS             complement(123..986)
                     /locus_tag="VJC18_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45888.1"
                     /translation="MVIEKTTDKTHSTLPVINPDTPPEDILPTPTTLEESIIDTYMNG
                     MQGTAYDPYASINDSLVKAVSPTITGSQNSAILAPAIEEPNQNDFLHDFSAEKPETDN
                     DVFLIDGKIFIVRSLGRDAIFSIVITERTDGHNMVVGVAVISKVEADRIKKLRPTAKV
                     PSSLERIINDPNRFVVIKKRHALVKEPLTGAIHKLSFKSLTNITGQQMTVGNDREINW
                     DSAQTHSWQSFADMVDEQTLSSESILPTPKGLRAIFFALPSHPFTGVVLVIDAKRSLK
                     ILNIPFHACPV"
     gene            complement(1155..2702)
                     /locus_tag="VJC18_10685"
     CDS             complement(1155..2702)
                     /locus_tag="VJC18_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45889.1"
                     /translation="MFYSGSAVPLSAFFNRQNELAWLLLRRDQLLKKAGQPTALLGMR
                     KSGKTSLLYKFLTNVQGPQIVTLSFSVPFNPTFQVFSNFLYKCLSFFLAEERISFSPD
                     DEQMDVVMGRVMEIPLSTKRKQMLLQGLQLYRQMQRQFRTEHIEAVLEFPATYVKVCK
                     KKAIIVWDEFQNLLDVSKDGKVSAGGDLLGRFREIWQRHDHIMYIVCGSEIHVMHAIL
                     NDESSPFFSHFQQLKIGPFSEEHITELLQTNGRQAGVVFSDAVCKQYFQTFGGIPFYA
                     QVIGDALVRLKIKKIAWRDVLYVVQQELWHQEGRLYLYFLNTFDKICANSGLKRAILI
                     ALSKKHMTVSELALVIQSSTGSVSNALPDLNVLISENEGRYYIQDPAFSLWLMAYDGA
                     AMILSPYLKGNEAEIKVASDLAQYGIHSYFSRGSRSAIDLLIEKNGKRAAVQVKKHSL
                     PAYLKKSEWQRLCGESVSWRMPPVIAVVDDQAVYYFDATKQKNFSVKGLLLQSARALE
                     NILLVLK"
     gene            complement(2869..>3144)
                     /locus_tag="VJC18_10690"
     CDS             complement(2869..>3144)
                     /locus_tag="VJC18_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45890.1"
                     /translation="LGTITDFVPTQQVAAQNDWNQNGVSDYDDVTPECSPATLQAQYT
                     TEKWEQMETDVCYELLPESTIIDSVELQKVLQNSHREPITNLRRQMF"
BASE COUNT          844 a          721 c          698 g          881 t
ORIGIN      
        1 agaaaccatg gctgttttgg cgaccatgct tcttttaaaa gagcctctta taagacttct
       61 cggggatctc cctcgtacaa ctcgctatcc acctgatttg cagctcctac tacctatgtg
      121 aattaaaccg gacaggcgtg aaacgggata ttcaatattt taagactgcg tttggcatcg
      181 attaccagaa ccacaccggt aaatggatga gagggtaacg caaaaaagat ggcccgtaaa
      241 ccttttggag tgggaagaat tgattcgctc gaaagagtct gctcatcaac catatctgca
      301 aaactttgcc aggaatgggt ttgtgccgaa tcccaattga tttccctgtc attacctaca
      361 gtcatttgct ggccagtgat attcgttaga ctcttgaatg aaagtttgtg aatcgcccct
      421 gtcaatggct cttttaccaa agcgtgtctc tttttaataa cgacgaatct gtttgggtca
      481 ttaatgatgc gttctaaaga acttgggacc tttgctgtgg gtcgcaattt ttttatgcgg
      541 tctgcttcaa ccttggatat tacggcgaca cctaccacca tattatggcc gtctgtgcgt
      601 tctgtgataa cgatgctaaa tatggcatct cgacccaatg atctcacaat aaatattttg
      661 ccatcaatta aaaaaacatc attgtccgtt tctggttttt ctgcagaaaa atcatgcaaa
      721 aaatcatttt ggttaggctc ttcaatcgca ggtgccagga tagcgctatt ttgagatcct
      781 gtgattgtgg ggctaacagc cttgaccaaa ctgtcattga tagaggcata tggatcgtaa
      841 gctgttccct gcataccatt catataggtg tctattattg attcttccaa cgttgttggt
      901 gtgggaagta tatcctctgg cggtgtgtca ggattgataa ccggcaaggt ggaatgagtt
      961 ttatcggttg ttttttcgat aaccatattt ggttatcgat tcatgtggtg caatgttgtg
     1021 tgtggtgcgt cgtttctgta tcaggggtga tacagaactc gtgggcggga cgactatcat
     1081 aggcagcgcg ctaacgcgac gtttgcctcg tgcccaatgg cattacgagt ttgccacctg
     1141 gttcgatggg ggcattattt caacaccaac aaaatatttt ctaaggctct tgcggattgc
     1201 agaagcaggc ctttgacaga aaaatttttc tgtttcgttg catcaaaata gtaaaccgcc
     1261 tggtcatcaa caacagcgat aaccggaggc atgcgccaag aaacagattc cccgcacaat
     1321 cgttgccatt cagatttttt tagatacgcc ggcaaggaat gttttttaac ctggaccgct
     1381 gcccgttttc cgttcttttc gatcagcaga tcaatggcac tccgggatcc ccgtgaaaaa
     1441 tagctgtgga taccgtactg ggccaaatct gaagccactt taatctctgc ctcatttccc
     1501 tttaaataag gcgacaatat cattgccgca ccatcgtagg ccatcaacca tagggaaaaa
     1561 gcggggtcct gaatataata acgaccctcg ttttcgctga tcagtacgtt cagatccggc
     1621 agagcatttg aaacggaacc cgtggatgat tgaatcacta aagccaattc actgacggtc
     1681 atgtgttttt tcgatagtgc aatcaaaatt gcccttttaa gaccggaatt ggcgcaaatt
     1741 ttatcgaagg tattcaaaaa atataaatac aaccgacctt cctgatgcca aagttcctgc
     1801 tgaacaacgt agagaacatc tcgccaggca atttttttga ttttcagccg cacgagcgca
     1861 tcaccgatca cttgtgcata aaatggtatg ccaccgaacg tttgaaaata ttgtttgcaa
     1921 acagcgtcag aaaaaaccac acctgcctga cgaccgtttg tctgtaacaa ctcggtgata
     1981 tgctcttctg aaaacggccc aattttcagc tgctggaagt gggaaaaaaa cggagacgat
     2041 tcatcgttca ggatggcatg catcacatga atctcgctac cacagacgat gtacatgatg
     2101 tgatcgtgcc gttgccaaat ctcacgaaaa cgacccaaca gatcaccccc agcactcact
     2161 ttgccatcct ttgatacgtc cagcaggttc tgaaattcat cccaaacaat gatggctttt
     2221 tttttgcaga ccttgacgta tgttgccgga aactccaaga cagcttcgat gtgctcggtt
     2281 ctgaattgcc tctgcatctg ccggtaaagt tgcaacccct gcaagagcat ctgttttctt
     2341 ttagtggaaa gtgggatttc cataacccgg cccatcacca catccatttg ttcatcatct
     2401 ggcgaaaaag aaatacgctc ctctgccaga aaaaacgata aacatttata cagaaaattt
     2461 gaaaatacct gaaatgtggg attgaaagga accgagaagg acaaagtcac aatctggggc
     2521 ccctgaacgt tcgtcaagaa tttgtaaagc aggctggttt tgccagactt ccgcattccc
     2581 aaaagagcgg tgggctgccc cgcctttttc agcaactgat cacgacgcaa caacaaccag
     2641 gctagttcgt tttgtcgatt aaaaaacgct gacaggggga ccgctgagcc ggaataaaac
     2701 atgcgggaac agtttaactc gcaataggct tgctgtcaag tatacagctg tatacttgac
     2761 agctgtaatc tatacagcaa tttgagtttt acaacaccct ttgcccgttg cgggcatctg
     2821 tggcggaagt gatacagaaa aaaatgggcg gcatcatcca gagggtgttt aaaacatctg
     2881 gcgtcgtaga ttggtgattg gttctctgtg actgttctgt aagacctttt gcaattcgac
     2941 agaatcgatg attgtcgatt ccggaagcaa ttcatagcag acatcagttt ccatttgttc
     3001 ccacttttcg gtcgtgtact gggcctgcag ggtcgcgggg gaacattccg gcgtcacatc
     3061 atcataatcg ctcacaccat tttgattcca atcgttctgt gcggccacct gttgcgtggg
     3121 tacaaaatct gtgattgttc ccag
//
LOCUS       DATDUN010000765         3108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_40479, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000765 DATDUN010000000
VERSION     DATDUN010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40479"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /gene="metG"
                     /locus_tag="VJC18_10695"
     CDS             <1..1817
                     /gene="metG"
                     /locus_tag="VJC18_10695"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HLD45891.1"
                     /translation="RPKFEHAWKQLNISYDQFIQTSELRHHASVSRLFEKIYESGDIY
                     PRDYEGWYCESCEAFYTEKDLVDGLCPNHKTKPKWLKEQNYFFKLTKYSDFLLNHIEN
                     NPDFILPKKRRNEVISFIKQGLEDISISRSSFNWGIPLPIKKDHVIYVWFDALINYIT
                     GIGYGEDNAQFAPRWENVTHIIGKDITRFHCIIWPCMLKSAGIKLPKTILGHGFVYLK
                     GEKMSKSLGNVVGPLDVTETYPDFGSDALRYYLMRGSSFGDDSDFTWDNFIERYNADL
                     ANGLGNLVSRTLGMVWRYQQGTIRPLPSSEIESTTLLSLAGKTFEVASNLIRPEVSGD
                     CCFHTALEKIWAFITQVDQYIDQKAPWTLSKEKRSEELSVVLTTLVEAVRLISLLVLP
                     FIPATTQKIWAGFGFDSLQALEGITCDSLNNTLAIKTPHQLKTDKLMLFPRIETKNER
                     TNGRMDDGRQTSGTASEQAKPESGRKETPVSSSASHDSRSTIHDPQGNLIDIADFGKL
                     DLRVARVLTAQKVEKADKLLRLEIEIDGEQRQIVAGIAEFYTPEDMIGKNIVVVANLK
                     PATIRGIESRGMLLAAKKGKELCLVTPEKLIATGAKVG"
     gene            complement(1821..>3108)
                     /gene="glpK"
                     /locus_tag="VJC18_10700"
     CDS             complement(1821..>3108)
                     /gene="glpK"
                     /locus_tag="VJC18_10700"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HLD45892.1"
                     /translation="GLAAEDIAAIGITNQRETTVAWQRSTGEPIHHAIVWQDRRTADV
                     CACLKKKGLLKKIRRTTGLVLDPYFSGTKMGWLMKNVLAARVLVKKNDLAFGTIDSWL
                     VWKLTAGAQHVTDVSNASRTLLMNLKTLAWDDSLLKIFGVPKQTLPKICSSSEIYGHT
                     KNIQGLCDGIPIAGIAGDQQAALFGQACFAPGEAKCTYGTGSFLLMNIGKKFVAPKTG
                     LLTTVAWKIGKQVNYALEGSAFIAGAAVQWLRDELKIISSANQIEELAKTVADNGGVY
                     FVPALAGLGSPHWDPYARGVICGLTRGSRQGHLARATLEGIAYSQYDILLAMQKESGK
                     KLKKLKVDGGAVANDLLMQFQSDILDADLIRPKVIETTALGAAFLAGLAVGMWSSQKE
                     IASTFGVDKHFVPGMKKAERQRLIAGWQSALQKARG"
BASE COUNT          787 a          879 c          749 g          693 t
ORIGIN      
        1 tgcggcccaa gtttgagcac gcctggaaac aactcaacat ctcttacgat caattcattc
       61 aaacctcaga actcagacac cacgcgtcag tctcgcgcct gttcgaaaaa atttacgaaa
      121 gcggcgacat ctatccccgc gattacgaag gctggtactg cgaatcctgt gaggcctttt
      181 acactgaaaa agacctcgtc gacggcctgt gccccaacca caagacaaaa cccaaatggc
      241 tcaaggaaca aaactatttt ttcaagctca ccaaatacag tgacttcctg ctcaaccaca
      301 tcgaaaacaa tcccgacttc atcctgccaa aaaaacggcg caatgaggtg atttcgttca
      361 tcaagcaggg tttggaggac atcagcatct cccgcagttc cttcaactgg ggcattcccc
      421 tgcccatcaa aaaagatcac gtcatttatg tgtggttcga tgccctgatc aattacatca
      481 caggcattgg ctacggtgag gacaatgccc aattcgcgcc gcgttgggaa aacgtcacac
      541 acatcattgg caaagacatc acacgctttc attgcatcat ctggccctgc atgctaaaat
      601 ccgctggcat caaactcccc aaaaccattc tgggtcacgg gttcgtgtac ctcaagggcg
      661 aaaagatgtc caaatcgctt ggaaacgtgg tcggccctct ggatgtcaca gaaacatatc
      721 ccgattttgg ttctgacgcg cttcgctatt acctgatgcg cgggtcaagt tttggtgatg
      781 atagcgattt tacctgggac aatttcattg aacgctacaa cgctgatctg gccaacgggc
      841 ttggcaacct tgtgtcacgc acactcggca tggtttggcg gtatcaacag gggacgatca
      901 ggcccttgcc ttcatcggaa atagaatcaa caacacttct gagccttgcg ggcaaaacct
      961 ttgaagtggc gtcaaacctg atccggcccg aggtctctgg tgactgttgt tttcacacgg
     1021 ctctcgaaaa gatatgggcc ttcatcacac aggtcgatca gtacatcgat caaaaagccc
     1081 cctggaccct ctccaaagaa aaacgctctg aggaactttc ggtcgtcctc accacgttgg
     1141 ttgaagcggt gcgcctgatc agtttgttgg ttctgccgtt catccccgcg acaacgcaga
     1201 aaatctgggc aggttttggt tttgacagct tgcaagctct cgagggaatc acctgtgaca
     1261 gcctgaacaa cacgctcgcc atcaagacac cgcaccaact caaaaccgac aagctcatgc
     1321 tgtttccgag gattgaaacg aagaacgaaa ggacgaatgg acgaatggac gacggacgac
     1381 agacgtcagg aactgccagt gagcaggcaa agccagaatc tggaagaaaa gaaacccctg
     1441 tgagctcatc agcaagccac gattcacgat ccacgattca cgatccacaa ggtaacctta
     1501 tcgacattgc cgactttggc aaattggact tgagagttgc cagagtgttg actgcgcaaa
     1561 aagtggagaa agcagataaa ctactgcgcc tcgaaatcga aatcgacggg gaacaacgcc
     1621 aaatcgtggc ggggatagcg gaattctaca cgccagaaga catgattggc aaaaatattg
     1681 tggtggtggc caacctcaaa ccggccacga tccgtggcat tgaaagccgt ggcatgttgc
     1741 tcgccgctaa aaagggcaaa gaactgtgcc tcgtcactcc ggaaaaactg atcgccacag
     1801 gcgccaaggt ggggtgaggg tcatcccctc gctttttgca acgcactctg ccaacctgcg
     1861 atcaaccgtt gcctctcggc ctttttcatg cccgggacaa agtgtttgtc cacgccaaac
     1921 gtgctggcaa tttccttttg cgatgaccac atccccaccg ccaaacccgc cagaaaagcc
     1981 gctcccaggg ctgtggtctc gatgactttt ggtctgatga ggtctgcatc aagaatatcg
     2041 gactgaaact gcatgagcaa atcattggcg acagccccgc catcgacctt gagttttttc
     2101 aatttcttgc cactctcttt ttgcatcgca agcaggatat cgtattgaga ataagcgatg
     2161 ccttcgagtg tggcacgcgc gaggtgtccc tgacgggaac cccgcgtgag accgcaaatg
     2221 acgccccgcg catacgggtc ccaatgcggc gaacccaaac ccgccagggc cggaacaaaa
     2281 tacacacccc cattgtctgc tacggttttg gccagttcct caatttgatt tgctgacgaa
     2341 atgatcttga gctcatccct gagccactgc acagcggccc ccgcaatgaa cgcggaaccc
     2401 tccagcgcgt aattgacctg tttgccaatc ttccaggcca cggtcgtgag caatcccgtt
     2461 ttgggcgcca caaatttttt gccaatgttc atgagcagaa acgagcccgt cccgtaggta
     2521 cacttagcct cgccgggcgc aaagcaggcc tggccaaaca gggccgcctg ctgatcaccg
     2581 gcaatccccg ctatggggat gccatcacaa agaccctgaa tgttttttgt gtgcccataa
     2641 atttctgagg aggaacagat tttgggcagg gtttgtttgg gaactccaaa tattttgagc
     2701 agtgaatcgt cccaggcgag cgtcttgaga ttcatgagca gggtgcgtga ggcgttgctc
     2761 acatcggtca catgttgggc tcccgctgtg agtttccaga ccagccacga atcaatcgtg
     2821 ccaaaagcca gatcattttt tttgaccagc actctcgcgg ccaacacatt cttcatgagc
     2881 caacccatct tcgtgcccga aaaatagggg tccaacacca ggcctgtggt cctgcgtatt
     2941 tttttgagga gccctttttt cttgagacac gcgcacacgt cagccgtgcg tctgtcctgc
     3001 cagacaatgg catgatgaat gggttcccca gtcgacctct gccacgccac cgtggtttcg
     3061 cgttgattcg taatccctat ggccgcgata tcttccgccg caaggccc
//
LOCUS       DATDUN010000766         1114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_159094, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000766 DATDUN010000000
VERSION     DATDUN010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159094"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VJC18_10705"
     CDS             <1..489
                     /locus_tag="VJC18_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HLD45893.1"
                     /translation="HVNASEVEGEDGVGTFFALNEAVLEKPVNLEIMEILLGAQANVN
                     RKNSQGQTALHRSVSRQYQNATELLLSHGADVNVQDTKSSDTPLMRLCAESPHTQPNE
                     AFFKMLITQGAKLDIENKFGDTALLIAARVRNTKMVDLLITLGADKNHVNKKGEGLKD
                     LL"
     gene            complement(503..>1114)
                     /locus_tag="VJC18_10710"
     CDS             complement(503..>1114)
                     /locus_tag="VJC18_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45894.1"
                     /translation="NVIDEGSVAQYGINFGGYGKGNFRKWGADPNSQTIIFAGWPVNF
                     DEHNLGMMVGIGAFNGKGNTAPSFAKADAATGQGKPLFFSFHGCLLPGIAEPNQGCFD
                     YHLDDAQMPDGTLLIDEYSESKYGILPDGYVDDAGDCSNLEDAGFADLANENYDVYKY
                     MPCINFKIIDGFDRDRYKNYYSSSNKFDYGDDPYGSSTCGYGN"
BASE COUNT          308 a          295 c          255 g          256 t
ORIGIN      
        1 catgtcaacg ccagcgaagt tgagggggag gacggtgtgg gcactttttt tgccctgaat
       61 gaggctgttc tggaaaaacc tgttaatctg gaaatcatgg aaattcttct gggggcccag
      121 gccaatgtga atcgcaaaaa ctctcaggga caaaccgccc tgcacaggag cgtctccaga
      181 caataccaaa atgccacaga acttttgttg tcgcatggcg cggacgttaa tgttcaagat
      241 acaaaatcgt cagacacacc gctcatgcgc ctttgcgccg aaagccctca cacacagccc
      301 aatgaggcct tctttaaaat gctcatcacc caaggggcca aactggatat cgaaaacaag
      361 tttggcgaca cagccctgct catcgcggct cgagtcagaa acaccaaaat ggttgatctg
      421 ctcatcacgc tgggggccga taaaaatcat gtgaacaaaa aaggggaagg gttgaaggat
      481 ttgttatagg tgattttttc gctcagttcc catacccgca ggtggaagag ccataaggat
      541 catcgccata gtcgaatttg ttactgctcg aataatagtt tttgtaccga tcgcgatcaa
      601 acccatcaat gatcttgaag ttgatgcacg gcatgtactt gtagacatcg taattctcat
      661 tggccaaatc cgcaaagccc gcatcttcaa ggttggagca gtctcccgcg tcatccacat
      721 agccatccgg caaaatgcca tatttgcttt cgctgtactc atcgataagg agcgtgccat
      781 cgggcatctg ggcatcatcc agatgataat caaaacaacc ctgattgggt tcggcaatgc
      841 ccgggagcag acaaccatga aacgagaaaa acagaggctt gccctgccct gtggccgcgt
      901 cggccttggc aaacgaaggc gccgtattcc ccttgccatt gaacgcacca atgccaacca
      961 tcatccccag attatgttca tcgaaattga ccggccaacc agcgaagatg atggtttgtg
     1021 aattgggatc ggccccccat tttctaaaat ttccctttcc ataaccacca aaattgattc
     1081 cgtactgcgc cacagaaccc tcatcgatca catt
//
LOCUS       DATDUN010000767         1399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_324024, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000767 DATDUN010000000
VERSION     DATDUN010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324024"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /locus_tag="VJC18_10715"
     CDS             complement(<1..>1399)
                     /locus_tag="VJC18_10715"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HLD45895.1"
                     /translation="AGFEDSPRDLPAWYARTLNASISFSNVVNNAGNNTGSSATTSVQ
                     STIDLNHDGVLDRVATQWTDEEGNAEGEETSSVTYGSYPPPYLLSTVENNQGGQTGIG
                     YRSLATVSPSGDWSELQDTPNVKHLANVIETLDGITGASSRLVFEYDDGKFDRGIFAG
                     FAGRSKTMSLDETIKGKTEYEYSLIEDLEPVITTQTQYDASSVSTGMGKGSSSNSFKR
                     RFMIETTYKAVSGDRSAFYLPSQRTITEYGGVSGSATSVIDFTWDEFGNLITYKHNGG
                     GDPSLAIDVHYEYVADSANSFFRIAQQTISGTSGAVSQTTYYYDNHSGATETLTQGVL
                     TQEDKSAGWTDGGEGLDDQRLSIQYGRGSRGEMTSVLDVATGEKIDQTFGFGGAVLET
                     QTNALGHKLVRSIDERGRIASIKDNNGLMLLTTYDDFNREIKKEVTGTDGKTQTLKEN
                     EYTRSAWPYYSQQKLY"
BASE COUNT          305 a          387 c          332 g          375 t
ORIGIN      
        1 tatacaattt ttgttgtgaa taatagggcc aggcagaccg cgtgtattca ttttccttga
       61 gcgtttgtgt cttgccatcc gtgccagtca cttctttttt gatctcacga ttgaaatcgt
      121 cataggtcgt gagcaacatc aaaccgttgt tgtctttgat agatgcaatg cggcccctct
      181 cgtcaatgct cctcaccaat ttgtgcccca acgcgttggt ctgcgtctcc agcaccgccc
      241 cgccaaagcc aaaggtctgg tcgatttttt caccggtggc cacatcaagc acagaggtca
      301 tttcaccacg actgccgcgt ccgtattgta tcgatagcct ctgatcatca agcccttcac
      361 ccccatcggt ccagcccgcg gatttgtctt cctgagtcaa gacaccctgg gtcaaggtct
      421 cggtagcacc ggagtggttg tcgtaataat aggtcgtttg cgacaccgcg ccgcttgtcc
      481 cactgatggt ttgttgggcg attctaaaaa aactgttggc actatctgcc acgtattcat
      541 aatgcacgtc gatggccaag gacggatcac cgcccccatt atgtttgtat gtgatgaggt
      601 ttccaaactc atcccaggta aaatcgatga cactggtcgc ggagccactc acgccgccat
      661 attccgtgat tgttctctgg ctgggtagat aaaacgcaga cctgtcccca gacactgcct
      721 tgtaggttgt ttcaatcatg aaccgtctct tgaaactatt ggaggaactg cctttgccca
      781 tccctgtgga gaccgaggaa gcatcatact gggtttgcgt ggtgatgacc ggctccagat
      841 cttcaatcag ggagtattcg tattccgttt tgccctttat cgtttcatcg agagacatag
      901 ttttggaacg ccccgcaaat ccagcaaaaa tcccccgatc aaatttgcca tcgtcatatt
      961 caaagaccag tcttgaactc gccccggtaa tgccgtccag cgtttctatg acgttggcca
     1021 gatgcttcac gttgggggtg tcctgcagtt ctgaccaatc tccagacggc gaaactgtgg
     1081 ccagagagcg gtatcctatg ccggtttgcc cgccctgatt gttttccacg gtgctgagca
     1141 aatacggtgg gggataagaa ccatacgtca cactgctggt ctcctcacct tctgcgttcc
     1201 cttcttcatc cgtccactgg gttgcaacac gatccaacac cccatcgtga ttgagatcaa
     1261 tcgtgctttg aaccgaggtc gtggcactac tgccagtatt attgccagcg ttgttcacaa
     1321 cattcgaaaa cgagatggag gcattaagtg tcctggcata ccacgcggga agatcgcgtg
     1381 gagagtcttc aaacccggc
//
LOCUS       DATDUN010000768        11401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_159156, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000768 DATDUN010000000
VERSION     DATDUN010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159156"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..455)
                     /locus_tag="VJC18_10720"
     tRNA            complement(384..455)
                     /locus_tag="VJC18_10720"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Gln,seq:ttg)
     gene            complement(540..2267)
                     /locus_tag="VJC18_10725"
     CDS             complement(540..2267)
                     /locus_tag="VJC18_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLD45896.1"
                     /translation="MSQQTSTQAKYILTVCLTLIFCVVVFGLWLPRQVRHDTEPQATV
                     ADQGLGTSPEAKKSELSQELLDYFKHHQLTNQSETDTLTEADLTERFEEISEDYITTA
                     EQIIQSRFKSPEALNASAEDFTVPKGLEEQVKFWTHIFGVYTNDHVIFYHSDDVGVVY
                     SVLDFSQISSEEGGLGAIKNRLISEEGARIKQILSHLDGKLKREKVKDGSYKTTISQT
                     GLTDEEKRIAKLLASSHMLETLDEKTLLENLAYRNGFSKRIKSALQASGAYMEEMQRI
                     FRERGLPPELTMIPFIESAFNLEAYSRAGAAGIWQFIEATGKRYLRIDEYTDERYDPI
                     LAAYAAATHLANEYEFLGGWALTINAYNTGPGRIQQAMKQLQTTDIANIIHSFKGSGY
                     GFDSRNYFPEFLAMLDVYRNKEYYFGDLKMAEKQEFAYVALPSPMNIKELARVAGINL
                     ETFADYNQGLKDEVINGDVLLPKGYLIKIPPTSKDNMLLAMQEQFDSVRYASHHVVET
                     GDSLKSIANRYALPIKQLAQFNGVLSNEDLTPGSIVRLPSRVDNVELTMDASHDQMVI
                     PDQVHTPVF"
     gene            complement(2395..3435)
                     /locus_tag="VJC18_10730"
     CDS             complement(2395..3435)
                     /locus_tag="VJC18_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLD45897.1"
                     /translation="MTRRILMMVVLLFGLVQMRCSAVSNAISDITSLDCDEWLQEEGD
                     STLNELCRDLEGYLDTDISSFTTAQSQAMPTADIAEEKRTYIMLTNSEGDPITDVVAD
                     DVTVEVSTDGGETYAEVAVDSVTNLADVTEVQASLGMLIDYSGSIMDSDLEDVNSGLG
                     LFYDNTFPTEASSAYQSAVIKFSNEVTVVQDFTSSKTALGAAVDDTSYARLSTSLYDA
                     IYDGVTEIIDEPTALNLLILFTDGVDNHSVHSKDEAIAQAVDHKVPVCVVGVGFADVS
                     TLREIAEDTGCFFIYKTLFSDLDLAFEVFSDQINGFYVIDLDDAFSETTGILRTTIEV
                     GEAEARVIEQTF"
     gene            3465..4184
                     /locus_tag="VJC18_10735"
     CDS             3465..4184
                     /locus_tag="VJC18_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45898.1"
                     /translation="MPQKIEKLLVVALKPEWSFLKQHYRFTSDSKHKELFFINDKNDT
                     ALLQIGLGRKKAGSVFLNFLADFDCHQVLHFGSSGALIPSLNPGDLFLAGSIRHQTQI
                     ITVDPTNIKTVTDRFPSIQTGSLLTVDAPLVKPHDKRDAHLKYSADTVDMESFAIANI
                     CAQKNVGYLSLRSVFDKREEDIESLGLPCDENGNFQPARFVGNLVTHPKLVVKIPSLQ
                     KRHHRMNQNFKEVLLWFLEKM"
     gene            complement(4249..5646)
                     /locus_tag="VJC18_10740"
     CDS             complement(4249..5646)
                     /locus_tag="VJC18_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD45899.1"
                     /translation="MQLLRKEFKQLQQWYSTPIRKTPCVLFGARQVGKSTLAKNFAES
                     TGRKVFTVNFWKDTEQKFPKIFTKKASAKDVLQKLELVFNTPIDTEKSILILDEIQEL
                     PVVYSLFKTFKEDTSLPIIATGSYLKLFLANQAEMEIPIGCTSEYLVTPLTFGDYLQN
                     KNKNLYEIYLHLPLDQPVEALCHNELMATYYEYLFTGGMPEVVSVFLETCEKQGVFVA
                     AELARKIQEQLISSYRNDFLTFNQKKFAVGRDIVGKLALTFSAVPRELAKYRESEVPV
                     GRFKFSSLGENATYKRVNHVFEYLSLSGLIIKSHIVKKPQYPLINEDETKNAFKCFYF
                     DVGVLHAALDVPFQSIVNDELSCYKGPIAENFVAQQLFSLGGHDLFSWKEKMGREQEV
                     EFLHPGAGLIPIEVKASKRGLPTKSLHVYINKYHPTFAIKIAPRNYGHANSVISMPIY
                     FIERINELGHLPGGH"
     gene            complement(5850..6371)
                     /locus_tag="VJC18_10745"
     CDS             complement(5850..6371)
                     /locus_tag="VJC18_10745"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HLD45900.1"
                     /translation="MHIVIDGYNLIRQVEALMRWERVSLESGRNALIGLLAQYKKNKR
                     HKITVVFDGVLTMSEFAPSFLQSGVHVCFSSFGQSADDVIRNLVLKEREKALVVSSDR
                     SIIDYAAKMGSAVMGSPQFYSKVINCRGNLDARDDDVATKVVHKRWKTQKKGPAKRLP
                     KKERRNQIKTNKL"
     gene            6620..6925
                     /locus_tag="VJC18_10750"
     CDS             6620..6925
                     /locus_tag="VJC18_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45901.1"
                     /translation="MSSWRDRLCGSEAINTSTDASEMSLKQYLAPQGNVSCGGGYYPI
                     NLNAANMKTFKGTFVLPTEEYAILLFKDDGSAVIYSDTEPKHARDDVLDEEGCTDPS"
     gene            complement(7071..8762)
                     /gene="ptsP"
                     /locus_tag="VJC18_10755"
     CDS             complement(7071..8762)
                     /gene="ptsP"
                     /locus_tag="VJC18_10755"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HLD45902.1"
                     /translation="MQTEYKIDKMLTLVGKGVSPGLAKGKAFVYIDVLQRDSEIYQID
                     TTQIGEEQARIDRAIDDVRQSLTIDANHIEGKLGKDSADIFLAHEAILLDSSVVGDLK
                     KTLEREQVNAEQVVRMVFRTLAGKFRKMNDAVLRERGDDIEDLSRRLLRSLSGIHAHS
                     LENLPENSVLVARRLLPSDTVFLSRSSTVAVLAEFAGPAAHAALLARELGIPCVGGIP
                     KLLQTVHTGDFVLVNGIEGIAVVNPDAQTLEKHEGAVGEVHERKATMIQDSCLKRTVT
                     LDGIDVSVLANVRSREDVVLAMKGGADGIGLFRTEPFFLSAKHFPSDKEFAEFLLDSL
                     RLARGKHVDIRLLDIGADKNPPYLHLPPEPDPFLGRRGVRVLREYPDLLDAQLRAMLL
                     VSQQFRIGILIPMVTTESDVVQIVMRLHELADEMGVHELPRIGAMIETPAAALTISFL
                     KRHVDFFSIGSNDLTQYTMAAGRENPLVTDYFVDDHPAVLRLIELVVKESGDTPVSLC
                     GELAGRIEVLLSLLKTGIRSLSVPAAMVPDVKNVIRQQVLFESHADLIDQTQIFR"
     gene            complement(8819..>11401)
                     /locus_tag="VJC18_10760"
     CDS             complement(8819..>11401)
                     /locus_tag="VJC18_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HLD45903.1"
                     /translation="VDGDGYTTCEGDCDDGSIAVHPEGTEICEDGIDQDCNGSDEICA
                     EDDNDDDGYTEDNGDCDDTNPAINPDAAEICDGIDNDCDGQIDEGFDVDGDGYTTCEG
                     DCDDGSIAVHPEGTEICEDGIDQDCNGSDEICAEDDNDDDGYTEDNGDCDDTNPAINP
                     SATEVCDGVDNDCDGQVDEGFDADSDGYTTCEGDCDDGSSAVHPDAAEICDDIDNDCD
                     GTVDENDVCDGTDDDQDGFVSDDDGGTDCNDSDASVYPGATEICGDGIDQDCSGSDVV
                     CEACSDGLDNDGDGAIDCADADCLQDDACVDTDVIVPGGIVCDVSIDINADGLPDALD
                     PAFGDADGDGIPDCCTDGNGDVVLINYDSDGDDVPNCREFALSSDPSISDIGFQGGAE
                     NFGCSLSLVFQTISEKTDRGLWFMILILASFGLFRIRKARMNLLKPQMIGLVVIVLLS
                     LARPAHALNVQTFAPADAVGSGYGLFTSQTLVPGQFVIGSFFNYAQHPLEIGFTNNNA
                     RVFGVVDKFITADLIATVGLFKHVNLTANMPINLYHDIAPTLIPNRDRGGPDLGDLRV
                     SSKIQFLDADTTAGNWGFAVVPFVTAPTGDASVYLGDTGITGGGTVIGEKKFGPHRLY
                     VNAGAQFKKEETLGNLVVGNAVTYGAGYQHQFSETHAISGIVEVQGSTAMNNLFGSEI
                     TTPVGARAILQKNFLASRNLHTYVGGGMGITSGYGASDFRVYSGLSFTFNAPHHKKVV
                     EAVTPEENFVGLVYFENDRDQFTQDNWATIEDAADFWKNHQSSPVLVVGHADSNWTND
                     YNMKLSERRALTVLKSLVKLGVPQDIIKVSYYGEEQPVDTNDTDAGRNNNRAAKMQII
                     VGEI"
BASE COUNT         3024 a         2896 c         2476 g         3005 t
ORIGIN      
        1 ccagatttgc gggcaggcac agcaccaaga taggcaccgt tgtcagaggt ggcacacacg
       61 taacgatctc caaaccctgc gcaagcagtc ccggttgcct ctaccccagc cattgccaga
      121 gctaagccct gttgccaagg atcacagaga ataggtgaaa aatattttgg gataaaaacc
      181 atctttttca actagtcttt tcatcgttca gcgtcaaaaa aagttgctag aaataatatc
      241 tttttaacta attgtaataa ttattatttt tgaacagtct ttgaattgac aaggaaaacg
      301 aaattcacgt ctcttttcgt tcacactttt tggcttctaa actaccaact actaactact
      361 aattcggcga agccgaattt ctgctggggt gctaggattc gaacctagga atgcgggaat
      421 caaaatcccg tggcttaccg cttgccgaca ccccagtagg gaggttatca atacgaacac
      481 cctccgaaga agggcctttt acttgagttt tgtttgcagt gtcaatcatg caatcaggtt
      541 tagaataccg gcgtgtgcac ttgatcggga atgaccatct ggtcgtggct cgcgtccatc
      601 gtcagctcga cattgtccac gcgactgggc aatctgacga tgctaccggg tgtgaggtct
      661 tcgttggaca aaaccccatt gaactgggcc aactgcttga taggcagtgc gtagcgattg
      721 gcaatcgatt ttaacgaatc cccggtttcg accacgtgat gtgaggcata gcggaccgaa
      781 tcgaattgtt cttgcatggc cagcaacata ttgtccttgg acgtcggggg tattttgata
      841 agatacccct ttggcaacaa cacgtcacca ttaataacct cgtctttgag accctgattg
      901 taatccgcaa aagtctctaa gttaatcccg gcaaccctcg ccagctcttt gatattcatc
      961 ggactcggca aagctacgta ggcaaattcc tgtttttcgg ccatcttgag atcgccaaaa
     1021 taatattcct tgttgcgata cacatcgagc atggccaaaa attcgggaaa ataattacgc
     1081 gaatcaaaac catagccact gcccttgaag ctgtgaatga tgttggcaat atcggtagtc
     1141 tgtaactgct tcatcgcctg ctgaatgcgg ccaggacccg tgttgtaggc attgatggtc
     1201 agggcccaac cgcccaaaaa ttcatactcg ttggccagat gggtcgctgc ggcataggcc
     1261 gccagaatgg gatcgtatcg ttcgtctgtg tattcgtcga tgcgcagata ccttttgcct
     1321 gtcgcctcta taaactgcca gatacccgcg gcccccgccc gtgaataggc ctcgagatta
     1381 aacgccgatt cgatgaaagg gatcatcgtc agttcgggag gcaggcctct ttccctgaaa
     1441 attctctgca tctcttccat atacgcacca gacgcctgaa gggcggactt gatgcgcttg
     1501 gaaaagccgt ttcgataggc aagattctcg agcagggttt tttcgtccaa ggtttccagc
     1561 atgtgactcg acgccaacaa tttggcaatg cgcttttctt catcggtcag acctgtctgc
     1621 gaaatggttg ttttgtatga accatctttg accttctcac gtttgagttt gccatcgaga
     1681 tgcgacaaaa tttgcttgat tctagcaccc tcttcgctga tcaaacgatt ttttatagcc
     1741 cccaaaccac cctcttcact ggaaatttgc gaaaaatcca aaacggagta gaccacaccc
     1801 acatcgtccg aatggtaaaa gatcacatgg tcattggtgt acacgccgaa aatatgcgtc
     1861 cagaacttga cctgctcttc aagccctttg ggaactgtga aatcctctgc cgacgcgttc
     1921 aacgcctcgg gcgatttaaa acgactctga atgatttgct ctgcggtcgt aatataatcc
     1981 tcggatatct cctcaaaccg ctctgtcaaa tccgcctcgg tcagtgtgtc tgtctcggat
     2041 tgattggtga gttggtgatg cttgaaataa tccagaagct cttgtgataa ttcgcttttt
     2101 ttcgcttcag gagacgtgcc caacccctga tcggcaacag tcgcctgagg ttccgtgtcg
     2161 tggcgcacct gtcgaggtag ccagagacca aaaacaacta cacagaaaat cagggtcaga
     2221 caaactgtga ggatgtattt ggcctgtgtt gacgtttgtt gactcatgca atgtaatccc
     2281 tcattatcca acctgatcga tttggctacc aattcctcgc aaaccaatca agcaccaata
     2341 tcggtcccaa cacacaacgg catgatatta tgaaattttt atttagtgtg cccatcaaaa
     2401 tgtctgctcg atgacccttg cctctgcctc acccacctcg attgtcgtcc gcaatatccc
     2461 agtagtttcg gaaaaggcat catccagatc gatcacataa aagccgttga tctggtcaga
     2521 aaatacctca aaggccagat ccaaatcaga gaaaagggtt ttgtagatga aaaaacaacc
     2581 tgtgtcttct gcaatctccc ggagtgtgct cacatccgca aaacccaccc caacaacaca
     2641 aacaggcact ttatggtcaa ccgcctgagc gatggcctca tccttgctgt ggacggagtg
     2701 gttgtcgacc ccgtcggtga acaaaatcaa aaggttgaga gcggttggct catcgataat
     2761 ctctgtcaca ccatcgtaaa tggcatcgta caaagaggtc gataacctgg catagctggt
     2821 atcgtctacg gccgccccca acgctgtttt tgagctggta aaatcctgaa cgaccgtcac
     2881 ctcgttggaa aacttgatca ccgcgctttg ataggccgag gaagcctcgg tgggaaaggt
     2941 gttgtcatag aatagaccca aaccggaatt gacgtcctcc agatcggaat ccatgatcga
     3001 gccactgtaa tcgatgagca tccccagtga ggcctgaacc tcggtcacat cggccagatt
     3061 ggtcacggag tctacagcaa cttcggcgta agtttcgcca ccatccgtgg agacttccac
     3121 tgtcacatcg tctgcaacaa catcggtaat gggatcgccc tcgctgttcg tcaacataat
     3181 ataggtgcgt ttctcttcgg cgatgtccgc tgtgggcatg gcctgggact gagccgtggt
     3241 gaaggaagat atgtccgtat cgagataacc ttcgaggtca cgacacaatt cattaagggt
     3301 gctgtcaccc tcctcctgca gccattcatc gcaatccaga ctggtgatgt cggaaatcgc
     3361 gttcgatacg gccgaacacc gcatttgaac cagaccaaac aacaacacaa ccatcatcaa
     3421 aatccgtctt gtcatgaaac ctccaaatgg gccataaggg ccgtatgccc cagaagatcg
     3481 aaaaactcct cgtcgtcgcg ctcaaacccg aatggtcttt tttaaaacaa cattatcgct
     3541 tcacgagtga cagtaaacac aaagaactgt tttttatcaa tgacaaaaat gacacagccc
     3601 tcttgcagat cggtttgggg cgcaaaaaag cgggaagtgt gtttttgaat tttttggctg
     3661 attttgactg ccatcaggtg ttgcactttg gttcgtcggg ggcattgatc cccagtttga
     3721 atccgggcga tctttttttg gcaggctcga ttcgccatca aacacaaatc atcactgtcg
     3781 atcccacaaa catcaaaact gtaaccgacc gttttccctc catccaaaca ggctccctgc
     3841 tgaccgttga tgcccccttg gtgaagcccc atgacaaacg cgacgcccat ctaaaatatt
     3901 ctgccgacac agtggacatg gagtcctttg ccatcgccaa tatttgtgct caaaaaaacg
     3961 ttggctacct gtccctgcgt tcggttttcg acaaacggga ggaggacatt gagtctttgg
     4021 gtctgccctg tgatgaaaac ggaaactttc agccagcgcg ctttgtgggc aatctggtga
     4081 cccaccccaa acttgtcgtt aaaatcccca gcctccaaaa acgccatcac cggatgaatc
     4141 agaatttcaa agaggtgctt ctgtggtttt tggagaagat gtgagcccaa aaaccagttg
     4201 gtcgagaaaa agctttgagg cgattcagag gtcctgtctt ttgacgcctt agtgtcctcc
     4261 aggaagatgg cccaactcgt tgatgcgttc aataaaatag ataggcattg aaatcacact
     4321 attggcgtgc ccataatttc ggggggcaat tttaatggca aatgtgggat gatatttatt
     4381 aatataaaca tgaaggcttt ttgttggcaa tcccctcttc gaggccttga cctcaatggg
     4441 gatcaatccc gcccctgggt gtaaaaactc cacctcctgt tcacgaccca ttttttcttt
     4501 ccaggaaaaa agatcatgcc ccccaagtga aaacaactgt tgcgctacaa aattttctgc
     4561 tatggggcct ttgtagcaac tcaattcatc attcacaatg gattgaaaag gaacatcgag
     4621 tgccgcatgc aacaccccca catcaaagta aaaacacttg aaagcatttt ttgtttcgtc
     4681 ttcattgatc aaagggtatt gtggtttttt gacgatatgg gatttgataa tgagacccga
     4741 gagtgaaagg tattcaaaaa catgattaac gcgtttataa gtcgcgttct ctcccaatga
     4801 tgaaaattta aaacgcccta ccggcacttc tgattctctg tatttggcaa gctccctagg
     4861 gacagcagaa aatgtcaggg ccagcttgcc aacaatgtct cgccccacag caaacttttt
     4921 ttgattgaac gtgagaaaat cgtttctgta tgaactgatg agttgttcct gaattttgcg
     4981 cgccagttcc gccgcaacga acacaccttg tttttcgcaa gtttctaaaa acacactcac
     5041 aacttctggc atcccgcctg taaataggta ttcgtaatat gtggccatca gctcattgtg
     5101 gcacaaggct tctacaggtt ggtcaagagg caggtgaaga taaatctcgt agagattttt
     5161 atttttgttt tgtagataat cgccaaaagt cagaggggtc accaaatatt ccgaggtaca
     5221 accgatgggg atttccattt cagcctgatt tgccaaaaaa agtttgaggt aactgcctgt
     5281 ggcaatgatg gggagcgacg tgtcttcctt gaaggttttg aacaatgagt acacgactgg
     5341 taattcctga atctcgtcca atatcaaaat ggatttttct gtatctatgg gtgtgttaaa
     5401 aaccagttcg agtttttgca ggacatcctt tgcgctggct tttttggtaa aaattttggg
     5461 gaatttttgt tcggtatctt tccaaaaatt gactgtaaac accttgcggc cggtggattc
     5521 cgcaaaattt tttgccaatg tcgatttgcc aacctgtctg gcaccaaaaa gaacacaggg
     5581 cgtttttcta ataggtgttg aataccactg ctgtaactgt ttgaattctt ttcttaataa
     5641 ctgcatattc gtattttact gccgtactgc cggtttttca acaaaataat cggcagtact
     5701 gccggttttt gggaaattag ttccaacgca ttgtaattac agtgttaatt tattccgaat
     5761 tttttcatct gcaaacaaat atttatttag cctaaattcc gcaagtcgga atttagaaaa
     5821 ccacgcaact tctgcagtga tcatgcccgt cacaatttat ttgttttgat ttgatttcgg
     5881 cgctcttttt ttggcaggcg tttggcaggg ccctttttct gcgttttcca tcttttgtgc
     5941 accacttttg tggccacatc gtcatcgcgg gcatcaagat tgcccctgca attgatcacc
     6001 ttgctgtaaa actgtggcga acccataacc gcactgccca tctttgcggc ataatcaata
     6061 atagagcgat ccgaactgac caccaacgct ttttctcgtt ccttaagcac aagatttcga
     6121 atcacatcgt cagcactttg tccaaaagat gaaaaacaaa catggacacc cgattgtaaa
     6181 aatgacggcg cgaattcact catagtgaga acaccgtcaa aaaccacggt gattttgtgt
     6241 cgtttgtttt ttttatactg cgccaataaa ccaatcaacg cgttgcgacc cgactccaaa
     6301 gacacgcgtt cccatcgcat gagtgcctcg acttgtctga tgagattata accatcgatc
     6361 acaatgtgca tacagtcacc atcatctttt gccgtgagac aagttaggca acatcgataa
     6421 acacgctcaa cgagcagatc aaacttatca aaatggtcat cctattgact ttagaccaat
     6481 ttgtatacga cactttttgc ctagttcccc ccgtcaaaaa agagctacca aatacacata
     6541 ataaattcaa catgttggtc tttgtttttt ttgcgttaaa tggcacgcgc cttgctaatt
     6601 tgaagatagg ggggtcagca tgtcatcttg gcgcgacagg ttgtgtgggt cagaggctat
     6661 caataccagc actgatgcca gtgaaatgtc tctaaagcag tacttggcac cgcaagggaa
     6721 tgtgagttgc ggaggggggt attaccctat caatttgaat gcggccaata tgaaaacctt
     6781 caaggggact tttgttctgc caacagaaga gtatgccata ttgttgttta aggatgatgg
     6841 ttccgccgtg atttattcgg acacagaacc caaacatgcc cgtgatgatg ttttggatga
     6901 agagggttgc acagaccctt catgattttt ttcacatctc cttggtaaaa agaaacccga
     6961 caatattatc ttccgtcggg tttttttttg gaacgcccgg cagagcgcac ccgaacatgt
     7021 ttatctggac cctattgaac aaaactggtt ttttgagaag tgcccaataa ctacctgaaa
     7081 atttgagttt gatcgatcag atcagcatgg gactcaaaca aaacttgttg ccttatgaca
     7141 ttcttcacat ctgggaccat tgcagcgggc acactgagag acctgatccc cgtttttagt
     7201 aagctgagaa gaacttcgat ccggccagcc agctccccac aaagagacac gggcgtgtca
     7261 ccggattctt tgaccactag ctcaatgagc cgcaaaacag ccggatgatc gtcaacaaaa
     7321 taatcagtca ccaacggatt ttctcgtcct gcggccatcg tgtattgggt gagatcattg
     7381 gaaccgatac taaaaaagtc cacgtgcctt tttagaaaag agattgtcaa ggccgctgcc
     7441 ggcgtttcga tcatggcacc aatgcgtggc aactcgtgaa cccccatttc atcagccaac
     7501 tcatgtaacc gcatcacgat ttgaaccaca tcgctctctg tggtcaccat ggggatcaga
     7561 attccaatac gaaattgttg agacaccaac agcattgccc tcagctgcgc atccaataga
     7621 tcgggatatt ctcgcaggac acgcacgcca cgtcgtccca aaaaaggatc gggctcgggt
     7681 ggcaaatgga gataaggcgg attcttgtcg gcgccgatgt cgagcaaacg aatatcaaca
     7741 tgttttcccc gcgccagacg aaggctatcc aaaagaaact ctgcgaattc tttgtctgaa
     7801 gggaagtgtt tggctgacaa aaagaaaggt tcggtgcgaa acaaaccgat cccatcagcg
     7861 ccacctttca tggccaacac aacatcttcc cgactacgaa cgttggccaa aacagaaaca
     7921 tcaattccgt ccaaggtcac tgtgcgtttg aggcaactat cttggatcat cgttgccttg
     7981 cgttcgtgca cttcgccaac agcgccctcg tgtttttcca gggtttgagc gtcgggattc
     8041 accaccgcga ttccctcgat cccgttcacc agaacaaaat ctcccgtgtg aacagtttga
     8101 agcaacttgg gaattccacc cacgcaggga atgccgagtt cgcgcgcaag aagcgccgca
     8161 tgcgctgccg gaccggcgaa ttctgcaagc acagcaacgg tggacgaacg cgagagaaac
     8221 accgtgtccg aagggagcaa tctgcgcgct acaaggacgc tgttctcggg gagattttcg
     8281 agactgtgtg cgtgaatccc agacaatgat cgcaacaacc tgcgggataa atcttcaata
     8341 tcatctcctc tctccctcag cactgcatca ttcatttttc tgaatttgcc ggccagggtt
     8401 ctgaacacca ttctgaccac ttgttcagca ttgacttgct ccctctctag ggtttttttt
     8461 aaatctccaa caactgagga atcaagcaaa atggcctcgt gagccagaaa gatgtcagca
     8521 gagtccttac ctaacttgcc ctcaatatga ttcgcatcga tggttaaact ctggcgcacg
     8581 tcgtcgatcg ccctgtcaat tctggcctgt tcctcaccaa tctgcgttgt gtctatttga
     8641 tagatttcgg agtctcgttg caacacgtcg atatagacaa acgccttccc tttggccagc
     8701 ccgggagaca ccccctttcc aaccagcgta agcatcttgt caattttata ttcagtctgc
     8761 atctgctgaa ctcctggaca actcaatcac gtgttgtgtt ggtttttgat gtctattttt
     8821 atatctcccc tacaatgatc tgcattttgg cggcccgatt gttattgcgt ccagcatctg
     8881 tatcattggt atcgacaggt tgctcttcac cataatagct gactttgatg atatcttgag
     8941 ggacgcccag tttaaccaaa gatttgagca cggtcagcgc ccggcgttcg gagagcttca
     9001 tgttgtaatc gttggtccag ttggagtcag catgaccaac aaccagaacg ggactgcttt
     9061 gatgattttt ccagaaatct gctgcgtcct caatggttgc ccagttatcc tgggtaaatt
     9121 gatccctgtc gttttcgaag tagaccagac caacaaagtt ttcttctggt gtcactgcct
     9181 ccacaacttt tttgtggtga ggagcattga aggtaaatga gaggcccgaa tacacacgaa
     9241 aatcagaagc accataaccg ctcgtgatcc ccatgccgcc ccccacataa gtatgcaggt
     9301 tccgagacgc caaaaaattc ttttgtaaaa tagcgcgagc acctacgggt gtcgtgattt
     9361 cactgccaaa caggttgttc attgcggtgg acccttgcac ttcaacaata ccagagatcg
     9421 cgtgagtttc tgaaaactga tgctgatagc cagcaccgta ggttacggca ttgcccacaa
     9481 ccagattccc caatgtttct tcttttttaa attgagcacc ggcgttgaca taaagacgat
     9541 ggggaccaaa ctttttttca ccaatcaccg tgccacctcc cgtgattcct gtatcaccca
     9601 aataaactga ggcatcacct gtgggggctg ttacaaaagg aaccactgca aatccccaat
     9661 tgccagcagt cgtatcagca tccaaaaact ggatcttgga gctaacacgc agatcaccaa
     9721 gatctggccc acctctgtca cgattcggga taagcgtggg agcaatatca tgataaagat
     9781 tgattggcat gttcgctgtg aggttcacat gcttgaataa gccaactgtg gcaatcaaat
     9841 cggctgtgat gaatttatcc accacgccaa agacgcgtgc attattattt gtgaaaccga
     9901 tttcgagcgg gtgctgggca tagttaaaaa acgatccgat aacaaactgt cctggaacca
     9961 atgtttgcga ggtaaacagt ccataaccag agccaacggc atcggcagga gcaaatgttt
    10021 gcacgttcaa cgcatgagcg ggacgagcca aactgagaag cacgatcacc accaaaccaa
    10081 tcatttgggg ttttaataaa ttcatacggg cctttctgat tcgaaagaga ccgaaggagg
    10141 caaggatcaa gatcatgaac cagagacctc tatcagtttt ttcggatatt gtttgaaaaa
    10201 caagggacaa agagcaacca aaattttcgg cgccaccttg aaaacctata tcacttatac
    10261 tgggatctga gctgagcgca aattcgcgac aattgggaac atcgtcgcca tctgaatcat
    10321 aattgatcaa gacgacatcg ccgttgccat cggtacagca atcaggaata ccgtcaccat
    10381 cagcgtcacc aaaagcagga tcaagcgcat ctggtaaacc atcggcgttg atatcaatgc
    10441 tcacatcaca cacaatacca ccgggaacaa tcacgtctgt atcaacacaa gcatcatctt
    10501 gtaaacaatc agcatcggcg caatcaatcg ctccgtcacc atcgttgtcg agcccatcac
    10561 tgcaggcttc acagacgaca tcgcttccac tgcaatcctg atcgattcca tcgccacaga
    10621 tttctgtggc tcctggataa acagaagcat cgctgtcatt gcaatctgtt ccaccgtcat
    10681 catcgctgac aaaaccatct tgatcgtcat ctgtgccatc acaaacgtca ttttcatcaa
    10741 ccgttccatc acagtcattg tcgatgtcat cacagatttc tgcggcatcc ggatgaacag
    10801 cgctggagcc atcatcacaa tcaccttcgc acgtggtgta accatcactg tccgcatcaa
    10861 agccttcatc gacctgccca tcgcagtcgt tatcaacccc atcgcagact tctgtggcag
    10921 aaggattgat ggcagggttc gtgtcatcac aatccccatt gtcttccgtg tagccgtcat
    10981 catcgttatc atcttccgca cagatttcat cggaaccgtt acagtcctga tcaataccat
    11041 cttcgcaaat ttctgttcct tccggatgaa cagcaatgga gccatcatca caatcgcctt
    11101 cgcacgtggt gtaaccatcg ccatcaacat caaagccttc atcgatttga ccatcacagt
    11161 cgttgtcgat accatcacag atttctgcgg catccggatt gatagcgggg ttcgtgtcat
    11221 cacaatcccc attgtcttcc gtgtacccgt catcatcgtt atcatcctcc gcacagattt
    11281 catctgaacc gttgcagtcc tgatcaatac catcttcgca aatttctgtt ccttccggat
    11341 gaacagcaat ggagccatca tcacaatcgc cttcgcacgt ggtgtaacca tcgccatcaa
    11401 c
//
LOCUS       DATDUN010000769         1136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_497612, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000769 DATDUN010000000
VERSION     DATDUN010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497612"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VJC18_10765"
     CDS             <1..1107
                     /locus_tag="VJC18_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HLD45904.1"
                     /translation="QKVKEQALHLATESWVRTFDAIPDLIAILDPNHRITLVNKAMAM
                     RLNKQPHDCTGLFCYNVVHGLDAPPSFCPHKKTMCDGQSHMEEVQESIIKGDFLVSTT
                     PIAGHDNHIIGAVHVARDITERKKLERELRCSIESQEEFLAIASHDLKTPLTDLYLTL
                     NFMKDVVMKTPMDKQQLVNTISHALKSGQQLVILLDELLDITRIRAGKLILEKENIDL
                     KAAVLEWVSIMSIEARQKGSQITVHADQSVVGEWDPTRINQVISNLLSNAIKYGGGKP
                     IEVTVLTDQPTNCAKLMVKDQGIGIPLEMQSKVFERFERAVSDQMIQGLGLGLYIVGQ
                     IVKAHGGSIRLESEPGSGSLFTVLLPMGEPFESV"
BASE COUNT          328 a          289 c          244 g          275 t
ORIGIN      
        1 caaaaagtaa aagaacaggc cttgcatttg gccaccgaaa gctgggtgcg caccttcgat
       61 gccatccctg atctcatcgc catactcgat ccaaaccatc gtatcaccct ggtgaacaag
      121 gccatggcca tgcgactgaa taaacaaccg catgactgta cgggcctgtt ttgctataac
      181 gttgttcacg gactggatgc acccccatcc ttttgccctc ataaaaaaac catgtgtgat
      241 ggacaatcac acatggagga agtccaagaa agcatcatca agggggattt tctggtcagc
      301 accactccca tcgccggcca cgacaatcat atcataggtg cggtccatgt tgcccgcgac
      361 atcactgaac gcaaaaaatt agaaagggaa cttcgttgtt ccattgaatc ccaggaggaa
      421 ttcctggcca ttgcctccca tgatttaaag accccgctga ctgatttata cttgacctta
      481 aacttcatga aagacgtcgt catgaaaact cccatggata aacaacaact ggtcaatacc
      541 atctcccatg cacttaaatc aggacaacaa ttggtcatat tactggatga attacttgat
      601 atcacccgaa tccgggcagg caaacttatt cttgaaaaag aaaacatcga tcttaaagct
      661 gcagtgttgg aatgggtttc catcatgtct atcgaagcac gccaaaaagg cagtcagatc
      721 accgtacatg ccgatcaatc cgtggtggga gagtgggatc ccacacgcat caatcaggtc
      781 atctccaatc tgctttccaa cgctataaaa tatggaggag gaaaacccat tgaagtcacc
      841 gtgcttactg atcaacccac caattgcgcc aaactcatgg taaaagacca gggaatcggc
      901 attcctttgg aaatgcaatc caaggttttt gaacgcttcg agcgagcagt gtctgatcaa
      961 atgatccagg gtctgggcct ggggctttat attgtaggcc agattgtaaa ggcccatggc
     1021 gggtctattc gcctggagag tgaacccgga agcggttccc tctttaccgt tttgcttccc
     1081 atgggtgagc ctttcgaaag tgtataaact tttgacactt tctaaaatca ttacac
//
LOCUS       DATDUN010000770         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_41096, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000770 DATDUN010000000
VERSION     DATDUN010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41096"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2573)
                     /locus_tag="VJC18_10770"
     CDS             complement(<1..>2573)
                     /locus_tag="VJC18_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45905.1"
                     /translation="QALYTTIIKAHRLDLVPLMTELWATCKTSADFQDVNHLFHWLLN
                     LSESEQKLALSFCYAKSIGRHEQTTSHESLRLGLRPLAEVLRTYVGRHQDFIRDYEQA
                     RTANGLCELLHSLETPDCGPLGQITAGTLLKQLANAPQGTVVADDVFELVYRVLLVTS
                     RVFTDEYIFDLPNSLDDHDWIKALEVLARKPQQYTQVTYSLSEARSNPNADIEALAQE
                     IDADLLLLDPTPASVILEYLLQIPTPEDVKLRYLLALIFAQLAQNPIYACHPALFTRL
                     MELSLDHKSSRVLTASMQGILALIKQQPPHGQEALLGKAQFEKAFSHLIKSKATHEAS
                     YADKGRAITLLEGAWACLDEEERDTYYGQVMRLATGFGLHQHFFAIVFSFLGSHADDV
                     SLSIKNKMFASADLARIIQNFLFLRKSPAEHLVALDTIALFEEHATGETLTAWQEFHE
                     QNVGVLEDSTRAYVRRYQAEGHQWEQATRLRAIEKLAKLLLDPQKGFVSTQIMVSVLK
                     HTPSEDVAKYLEVLLAHEGSVEAYLTGTQIQDASTEAQLLRQSRFSVQKMRLIFLIAS
                     VHPRCVSPELLEETIQRFNPNTTQGMTHFVARDMGRALREPKFHDLLDKAWQRLGFHS
                     IEMRDGGNGLSTLPYFMETYLAPTLVDFMRQAADMVIDLTSTTENAPSIPPTDPFSIR
                     HDSITNLLGQYPVVSGPVQRGRSFVYQLSDGRQLVFKCLRVGQTPSSLARELEWLRDT
                     RDIGLRSSLPQPLLDKSDKPLLFQLSDAPLHFVNTNPALDFDASAAIAFIAPGGARDY
                     YRYLDDVSLNDATFMRALTDAVHDLFRLDESLDVLHTAPIALFHNNETGAKFRAHDAG
                     R"
BASE COUNT          601 a          631 c          701 g          640 t
ORIGIN      
        1 taccgtcccg cgtcgtgtgc cctgaatttg gctccggtct cgttgttgtg aaacagggcg
       61 atgggggctg tgtggagaac gtccaaactc tcgtccaatc tgaatagatc atgaacagca
      121 tccgtcaggg cgcgcatgaa agtggcatca ttcagactca catcgtcaag ataccggtag
      181 taatcccgtg ctccccccgg ggcgatgaag gcgatggcgg cactcgcgtc aaaatcgagt
      241 gcggggtttg tgttcacaaa gtggagtggg gcatcactga gctgaaacaa aagtggcttg
      301 tctgatttgt ccagcagtgg ttggggcaga gaggacctga gaccgatgtc ccgtgtgtct
      361 ctgagccact ccagctcacg tgccaaggac gatggcgttt gaccaactcg caggcatttg
      421 aacacaagct gacgcccatc actgagctga tagacaaaac tgcgtcccct ctgcacaggc
      481 ccactcacca ccgggtattg gccaagaaga tttgtgatgc tgtcatgacg aatactgaac
      541 ggatctgtcg gagggatgct gggcgcgttt tctgtagtgc ttgttaaatc aatgaccatg
      601 tctgccgctt ggcgcatgaa atcgacgagg gtaggtgcaa ggtatgtttc cataaaatag
      661 ggcagggtgc tgagcccatt gcccccatcg cgcatctcga ttgagtggaa tcccagtctc
      721 tgccaagcct tgtccagcag atcatgaaat ttcggctctc tcagggcccg ccccatatcc
      781 ctggcgacaa aatgcgtcat gccttgagtc gtgttggggt taaatcgctg gatggtttct
      841 tccaacagct ctggcgacac acaacgagga tggacactgg caatcaaaaa tatcaggcgc
      901 attttttgca cggaaaaacg actttgtcgc aacaactggg cctcggtaga ggcgtcctga
      961 atttgggtgc cggtcaaata ggcctcgaca ctgccttcat gggctaaaag gacttccaga
     1021 tatttggcaa catcttcgct gggagtgtgc ttgagcacgg agaccatgat ctgagttgag
     1081 acaaaaccct tttgtggatc gagtaacagt ttggctaatt tctcgatggc cctgagtctg
     1141 gtggcttgct cccactgatg cccctcagct tgatagcgtc tcacgtaggc ccgtgtggag
     1201 tcctccagaa caccaacatt ttgttcatga aactcctgcc aggctgtcag tgtctcacct
     1261 gtggcgtgtt cttcaaaaag cgctatcgta tccagggcca ccaggtgttc tgcgggagat
     1321 ttcctgagaa agagaaaatt ttgtatgatg cgcgcgaggt ctgccgatgc gaacatcttg
     1381 ttcttgatgg ataggctgac gtcatcagca tgactgccca gaaaagaaaa gacgatggca
     1441 aagaaatgct gatgaaggcc aaaccctgtg gccaatcgca tcacttgtcc gtaataggtg
     1501 tctctttcct cctcgtcaag gcaggcccag gcaccttcca aaagggttat ggccctgcct
     1561 ttgtcggcat aactggcctc atgagtggct tttgatttta tgagatgcga gaatgccttt
     1621 tcaaactggg cctttcccag gagcgcctcc tgcccatgag gcggttgttg ttttatcaac
     1681 gccaggatcc cctgcatgct ggctgtgagc acgcggctgg atttatgatc gagtgacaat
     1741 tccattaatc ttgtgaacaa tgccgggtgg caggcgtaaa ttggattctg ggccagttgt
     1801 gcaaaaatca gagccagtaa gtatcgcagc ttgacgtctt ccggtgtcgg gatttgcagt
     1861 aaatattcca gaatgacaga cgcgggtgtg ggatccagca ggagcagatc agcatctatt
     1921 tcttgagcca aagcctcgat gtcagcattg gggttgctcc tggcctcgga gagggaatat
     1981 gtgacttgtg tgtattgctg aggttttctt gccagcactt cgagggcttt gatccaatcg
     2041 tgatcatcta acgaattggg caaatcaaaa atatattcat ccgtgaagac tcgggacgtg
     2101 accagtagta cccgataaac caattcgaag acatcatctg cgacgaccgt gccttgtggt
     2161 gcgttggcaa gttgtttgag gagggtgcct gcagtgattt gtcccaatgg cccgcaatca
     2221 ggggtctcca atgagtgtaa tagctcacac agaccatttg ccgtgcgtgc ctgttcataa
     2281 tcgcggatga aatcctgatg ccttcccaca tacgtgcgta aaacttccgc caacggacgc
     2341 agtccaaggc gtagcgactc atgacttgtc gtttgttcgt gacggccaat agacttggcg
     2401 taacaaaacg ataaggctaa cttttgttct gactcggaca ggttcaatag ccaatgaaac
     2461 aaatggttca cgtcctgaaa atccgctgat gttttgcatg tggcccacag ctcggtcatg
     2521 agcggcacca gatcgagtcg atgcgccttg atgatggtgg tatacaaggc ttg
//
LOCUS       DATDUN010000771         3046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_159409, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000771 DATDUN010000000
VERSION     DATDUN010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159409"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VJC18_10775"
     CDS             <1..626
                     /locus_tag="VJC18_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLD45906.1"
                     /translation="LHLGYQSAQNVATIVSKVKKLKPHAVLITGDLIDSNAFLPDDLL
                     AFNELSVPIYYTFGNHEFYIKKGVPEHAISQTNIQLLRNQRAELDELQIWGVDDNRHD
                     DLEAQLKQISIDKQGFTILMKHSPTQINKAAKRGVDLVLSGHTHGGQIFPFNFLVRIF
                     YHYVAGLYEVKGTKLYVTEGTSFWGPPIRLGTHNEIAVIELKPTKTR"
     gene            708..2033
                     /locus_tag="VJC18_10780"
     CDS             708..2033
                     /locus_tag="VJC18_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD45907.1"
                     /translation="MTKGTYITRLLEAELIDFISNQQEPFNTILLGGPRQCGKSTMLK
                     HLFPPDRHIHINLAEESAFCDAIDTTQCFDDLTVLIESRYHTKIGSGRIIIFDEAQLS
                     HKLGNYVRFFKENWKTQTVIITGSTLSNLFTHTEKPTGRVVEFVLRPFNFSEFLDALG
                     QDSLRERLKNWTPEKPFSDTVHRELVTQLQSYLVVGGLPDVVLTYSRQGNHTKLLADI
                     FAFYKRDFQSKLSSENLTSIFSQVFARIAASTGSEIKLSSIIASSSPGYRKLRDVLTI
                     MEMWHQIIRVDYETYQLTKFGTTTPKRYLFDQGIRYLLNPSRFVGLDLLDSAHPKREE
                     IGGLLENFVLTELISTNSPFAIRSWAKTHQSGMVDFLYNTEKDAFAIEVKAALSLDRK
                     HFSALLSYHEVYPNTHLILVNLDKGGVYQTKEGATITSVPAYAICRALI"
     gene            complement(2082..>3046)
                     /locus_tag="VJC18_10785"
     CDS             complement(2082..>3046)
                     /locus_tag="VJC18_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45908.1"
                     /translation="LAAKGFLTFYVNLYAATTKKKFIDLYARAITTGSQGHLDRVVKQ
                     LKDLLPRMIPKIIIKGDGSPEYEFDYSKKETEVSPLLEDLYGAVEKLALKRNKRAVVV
                     FDEFQEILNYPDQEIERGMRGFIQAQKKTAYVFMGSRRHLMQLIFNDIERPFYKSGRM
                     FPLKKIDPIEFAKFIQDSFQKGKIHLQQDFIETLLAVTDCHPYYTQMLCSVLWDRCSS
                     KQCLNKTDIEKAIKELIEREAYTYTTIWDELSLQKRELLETLARNNNIKLYSKEFLSQ
                     LEENDINAIQRNTKALEETGLIYKDGGKWLFSDIFFQRWIISQI"
BASE COUNT          850 a          733 c          605 g          858 t
ORIGIN      
        1 acctgcattt gggttaccaa tcagcacaaa atgtcgcgac catcgtttca aaggtcaaga
       61 aactcaaacc ccatgcagtc ctcatcaccg gcgacttgat tgatagcaac gcgtttttgc
      121 ctgatgatct actcgccttc aacgagcttt ctgtcccgat ttattacacg tttggcaatc
      181 acgagtttta cataaaaaaa ggggtccctg aacacgccat ttcacagacc aacatccagt
      241 tgcttcgcaa tcaacgtgct gagttggatg agttgcaaat atggggcgtg gatgacaacc
      301 gacacgacga tcttgaggct caactcaaac agatttccat cgacaagcag ggcttcacga
      361 tcctcatgaa gcactcacca acccagatca ataaagccgc taagcgtggg gttgatctgg
      421 tcctgtctgg ccacacacat ggcggacaga tctttccgtt caattttttg gtaaggattt
      481 tttaccacta cgttgccgga ttgtacgagg ttaagggaac caaactctat gtcaccgagg
      541 gcacatcctt ctggggtccc cccatccggt tggggacgca caacgaaatt gcggtgattg
      601 aattaaaacc caccaaaaca cgatgacgga ggtaatttat ttactataaa actaatcatt
      661 attattgcca tattatatat aatattgtat atttttagtg tactattatg acaaaaggaa
      721 cctacatcac tcgcctctta gaggctgaac taattgattt tattagtaac caacaggaac
      781 ctttcaacac tattctactt ggtggtccca ggcaatgtgg caaatcaaca atgctcaaac
      841 atctctttcc accagaccgt cacattcaca tcaatctggc ggaagaaagc gccttttgcg
      901 acgccatcga taccacacaa tgctttgacg atctgacagt cctcattgaa tcacgttacc
      961 acacaaaaat cggatctggt cgcatcatca tttttgatga ggcacagctc tcacacaaac
     1021 tgggaaacta cgtccgattt tttaaagaga attggaaaac acaaacagtc atcatcacag
     1081 gctcgacact atccaacctt ttcacccaca cagaaaaacc aaccggccgc gtggtcgaat
     1141 ttgtattgcg acctttcaac ttcagcgaat ttcttgatgc gttgggtcag gactcattgc
     1201 gtgaacgctt gaaaaattgg acaccggaaa aacccttttc tgataccgtc catcgtgaat
     1261 tagtgacaca gttacagtcc tatcttgttg tgggaggtct acccgatgtt gtcctcacct
     1321 attcccgaca aggcaatcat acaaaactat tggcagacat cttcgccttt tataaaagag
     1381 attttcaatc aaaactttcg tcagaaaact tgacctccat cttctctcag gtctttgccc
     1441 gaatcgccgc gtccaccggt tcggaaataa aactatcgag catcattgcc tcctcatcac
     1501 ccggctaccg aaaactcagg gatgtgttga ccatcatgga aatgtggcac cagatcatcc
     1561 gtgtggatta cgaaacatat caactaacaa aattcggaac aaccacgccc aaacgatacc
     1621 tctttgatca gggcatcagg tatctgctca atccctcacg atttgtcggt ctggatctgc
     1681 tggatagcgc ccaccccaag cgtgaagaaa ttggtggcct attggaaaat tttgtgctca
     1741 ctgagttgat ttcaacaaac tcgccctttg cgattcgttc gtgggccaaa acacatcaat
     1801 cgggaatggt tgattttctg tacaacacag aaaaggatgc ctttgccata gaggtcaaag
     1861 cggcattgag tctggacaga aaacattttt ctgcgttgtt atcctatcac gaagtgtatc
     1921 caaataccca cctgatcctg gtcaatcttg ataaaggtgg cgtctaccag acaaaagagg
     1981 gagcgacaat cacatccgta cccgcgtatg cgatctgtcg cgcactcatt tgaccagcgc
     2041 cccaatgctg gcacgcgata ggaaatcaac tactgcgttt ttcaaatctg gctgataatc
     2101 catctctgaa aaaaaatgtc tgaaaacagc cactttccac cgtctttata aatcaacccc
     2161 gtttcttcaa gagctttggt atttctttgt atggcattga tatcgttttc ctcaagttga
     2221 gacaaaaatt cttttgagta caatttgata ttgttgtttc tcgccaaagt ttccagaagt
     2281 tctctttttt gcagagacaa ttcgtcccat atggttgtgt atgtgtaagc ctcacgctca
     2341 atcagttctt taatggcctt ttcgatatcc gttttattca gacattgttt tgaagaacac
     2401 cgatcccaga gaacgctaca aagcatttgg gtataatagg gatggcaatc agtcaccgcc
     2461 aaaagtgttt cgatgaagtc ctgttgcaaa tgaattttcc ctttttgaaa agagtcttga
     2521 atgaattttg caaattctat cggatcaatt ttctttagag gaaacatgcg cccgctcttg
     2581 taaaacggtc gttcgatatc gttaaaaatc agttgcatga gatgcctgcg acttcccata
     2641 aaaacgtagg ctgttttctt ttgagcctgg atgaatccgc gcataccgcg ttcgatttct
     2701 tgatcaggat agttcaggat ttcttggaat tcatcaaaaa caacaaccgc ccgcttgttt
     2761 ctctttaggg caagtttttc gacagcgccg tacaaatctt ccagcaacgg ggacacttct
     2821 gtttcttttt tgctgtagtc aaattcatat tcgggcgagc cgtctccctt aataatgatt
     2881 tttggaatca tgcgtggcaa caaatctttg agttgcttca caaccctgtc taaatgacct
     2941 tgcgaaccgg tagtgatggc tctggcataa agatcgataa attttttctt ggtggtggcc
     3001 gcataaaggt tgacgtaaaa tgtcaaaaag cctttcgcgg ccaggt
//
LOCUS       DATDUN010000772         1726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_373425, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000772 DATDUN010000000
VERSION     DATDUN010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373425"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1726
                     /locus_tag="VJC18_10790"
     CDS             <1..>1726
                     /locus_tag="VJC18_10790"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HLD45909.1"
                     /translation="AHEGQKRKSGEPFLIHPLEVAKILTQLKMDISSLIAAILHDTVE
                     DTILSYDDIKASFGVDVAEIVDGVTKISKIKISNQEDRQAENYRKMIIAMSKDIRVIM
                     VKLADRINNMRTLQFMSEARQVGIAQETLDIYAPIAGRMGIYQFKEELEELSLKFLKP
                     EIYSQLATTTKRLEKLRELYMARVKDALTNSLRVSIPQFDIFGRIKRPYSVYRKMLAQ
                     QIELDDVHDLMAFRVMVSSIEQCYEVLGIIHSLWKPIQGRFKDYIAMPKANSYQSLHT
                     TVLCFDAERVEFQIRTHEMNEIADKGIAAHWKYKDDGRLDTKDEAKFRWLRHLMDWQS
                     ELKDSLEFVDTFKLDLFEDEIFVFTPNGDIKNLPFNATPVDFAYSIHSNVGERCAGAR
                     VNGRIVSINHKLDSGDTIEIITKKDHVPSKDWLDFVVSTRAKTKIRGFIRKQQRTKSI
                     LIGRNIFETACQKAKITPPRITKDPAFKTYLESKDLLDIDDFYQDLAYAKINAKEVIG
                     LVCPKEEPLPTQEQENENVIKKIFNKISARHKNIILVDSMDDILVVFGKCCSPVRGDP
                     IVGYVTRGR"
BASE COUNT          488 a          507 c          378 g          353 t
ORIGIN      
        1 cgcccacgaa ggtcaaaaaa gaaaatcagg cgaaccgttt ctcattcacc cgctcgaagt
       61 ggccaagatc ctcacgcaac tcaaaatgga catttccagt ctgatcgccg ccattttgca
      121 cgacaccgta gaagacacta tcctctccta tgacgacatc aaggcatcct ttggcgttga
      181 cgtcgccgaa atcgtcgatg gcgtgaccaa aatttccaaa atcaagattt ccaatcagga
      241 agatcggcag gcagaaaatt accgcaaaat gattatcgcc atgtccaagg acatccgcgt
      301 catcatggtc aaactggctg atcgcatcaa caacatgcgc accctgcaat tcatgtcgga
      361 ggccagacaa gtcgggatcg cgcaggaaac cctcgacatc tacgccccca tcgccgggcg
      421 catggggatt tatcaattca aggaagaact cgaggaactc tctctcaaat ttttgaaacc
      481 cgaaatctat tcccaactcg ccaccaccac caaacgcctc gagaaacttc gcgaactcta
      541 catggcccgc gtcaaggatg ccctgaccaa ctcactgagg gtctcgattc cccagttcga
      601 catctttgga cgcatcaaac gtccttacag cgtttatcgc aaaatgctgg cccaacagat
      661 cgaactcgac gacgtgcatg acctgatggc cttccgcgtg atggtctctt ccattgaaca
      721 atgttatgaa gtgctcggga tcattcactc cctttggaaa ccaattcaag gacgtttcaa
      781 ggattacatc gccatgccca aggccaactc ctatcagtca ctgcacacca ctgtcctctg
      841 ttttgatgcc gaacgcgtgg agttccaaat ccggacacat gaaatgaacg agattgccga
      901 caaaggaatc gccgctcact ggaagtacaa ggacgacggc cgtctcgaca ccaaagacga
      961 ggccaaattc cgctggctcc gtcatttgat ggattggcaa tcggagctaa aggattcgct
     1021 cgaattcgtc gacaccttca aactcgattt gttcgaggac gagatttttg tctttacgcc
     1081 caacggcgac atcaaaaacc tccccttcaa cgccaccccc gtggattttg cctattccat
     1141 tcattccaac gtgggggaac gctgtgcggg ggccagggta aacggacgca tcgtctcgat
     1201 caaccacaag ctcgacagcg gcgacacgat cgaaatcatc accaaaaaag atcatgtgcc
     1261 cagcaaggac tggctcgact tcgtcgtgag cacgcgggca aaaaccaaaa tcaggggatt
     1321 catccgtaaa cagcaacgca ccaagagcat cctcatcggc cgcaacatat ttgaaacagc
     1381 gtgtcagaag gccaagatca cgccccccag aatcaccaag gatcccgctt tcaagactta
     1441 tctcgaaagc aaagacctgc tcgacatcga cgacttttat caggatctcg cctacgccaa
     1501 gatcaacgcc aaagaagtga tcggactggt ctgccccaaa gaagagcccc ttcccacaca
     1561 agagcaggaa aacgaaaacg tcatcaaaaa aatcttcaac aagatttcgg cgcgtcacaa
     1621 aaacatcatt ctggtggatt caatggacga cattctggtc gtcttcggca aatgttgttc
     1681 ccccgtacgg ggagacccca ttgtgggtta tgtcacccgc ggacgc
//
LOCUS       DATDUN010000773         3715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_448621, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000773 DATDUN010000000
VERSION     DATDUN010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448621"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VJC18_10795"
     CDS             <1..773
                     /locus_tag="VJC18_10795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLD45910.1"
                     /translation="SWHRRVMDPIIHFLERRSEACLTYSSVGKAYYERLGIDSHKIFV
                     ALNVVDTEQRLKDMACFDRSLLYRDIHSRFDFVVLYVGVLTKEKKVDVLLKAFAELEK
                     FVSQEVCLLIVGKGEDDARLRSLAREWNIRNIQFVGEQRDGVSQYFFKADVMVLPGLG
                     GLAISDALLHGVPVIATAGDGCEVDLINKNNGVLDEDLNEERLCGYLKKMCEDQPYKQ
                     RLKSHAQDIINNRYNIHTYVQGWVDCLSFVQPVMTRKS"
     gene            776..2929
                     /locus_tag="VJC18_10800"
     CDS             776..2929
                     /locus_tag="VJC18_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HLD45911.1"
                     /translation="MKQIIQSFKTGEIWLADVPVPILKPEGVVVETHHSFISSGTEKM
                     LVSFAQKNLLGKACAMPDQVKKVIQKIKTEGLFETWEKVQAKLDQPIPLGYSCAGVVV
                     EVGQGVKGLVNGDRVACAGAGYANHAEFNYVPKNLLVKIPDKVSFRDASCATVGAIAL
                     QGVRQCDVRVGETVCVMGLGLLGLFAVQILKASGAKVVGFDPDARRCSLAQELGADSV
                     VKDNLVATCDAFSKGNGVDGVLITAATNSNEPVELAGQIARHKGKVVVTGMVGMNIPR
                     DVFYKKELDFKLSMSYGPGRYDTNYEEKGHDYPYGYVRWTEQRNIEFFLDLIADGHVT
                     PSKMITHEYDIAEGLKAYDLLLGREKSEYLGIVIGYGKSDGTTRVPERKVICGQNHQV
                     TDCVVGMIGMGNFAKAVLLPALQKQNHISLRCLCATTGIDITAVAKKTGFEYVTTDSA
                     ELFADPRINTVVIATRHDSHAGLVVKALEAGKNVFVEKPLALNRDELEAIKQAVQIHS
                     GILTVGFNRRFSPHAKLLRDYLAKKTSPAVVNYRINAGHIPYDSWVHDPIKGGGRIIG
                     EVCHFIDFVSYILSAMPRTVQACSVGGDNAERQAADNVSINIEYSDGSLANFIYTSFG
                     SRDHPKEQGEIFVDESVCLMKDFVSTECFGKLGRKKLKGKQSKGFQEEFLAFFSAVIN
                     KHQHPIPLDEIFVTTEATFLVSEAIKTRETLLITQ"
     gene            3016..>3715
                     /locus_tag="VJC18_10805"
     CDS             3016..>3715
                     /locus_tag="VJC18_10805"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HLD45912.1"
                     /translation="MPLCPKKIFIISSCSLKPELSFPVANRKHYLGSGAFLFNNKQNT
                     LVGRDWDFVKNDKLWSYRLHYQDWLLEDDCPVDDWVNKWIDDNSPFKGNGWEAYPISL
                     RMVTWIKLLAQGRLSDSAKIRLSLAQQASVLYARREYHLLGNHLLENLFALYLVSHVC
                     CKTSQQQIIYDQLVKEIDAQFLADGGHYELSPMYHAILLERILDILNLTRNRPLSQNV
                     YGKLIDVAHKGLNWL"
BASE COUNT          990 a          664 c          969 g         1092 t
ORIGIN      
        1 aatcatggca tcgtagagtc atggatccga tcattcactt tttagagcgt cgttcagagg
       61 cctgtttgac ttacagttct gtgggcaaag cgtattatga acgccttggt attgattccc
      121 ataaaatctt tgtggcgttg aatgttgtgg ataccgagca acgactcaag gatatggctt
      181 gttttgaccg ttcccttctt tacagagaca ttcattctcg gtttgatttt gtggtgttgt
      241 atgttggggt tcttacgaaa gagaaaaaag tggatgtttt gttgaaggcc tttgcggaac
      301 tggaaaaatt tgtgtctcaa gaagtgtgct tgttgattgt tggcaaggga gaggacgatg
      361 ccaggttacg ctctttggcg cgtgaatgga acatcaggaa tattcaattt gttggagaac
      421 agagggatgg ggtttcacaa tattttttca aggccgatgt aatggttttg cctggattgg
      481 gaggattggc catatctgac gcattgctac atggggtacc ggtcattgca acggcgggag
      541 atgggtgtga ggttgatctg atcaataaaa ataatggcgt tcttgacgaa gacctgaatg
      601 aagaacgcct gtgtggttat ttgaaaaaaa tgtgtgagga tcagccgtac aaacaacgtc
      661 tgaaaagcca tgctcaggat atcattaata acagatacaa cattcacact tatgttcagg
      721 ggtgggtgga ttgtttgtcg tttgtacaac ctgtaatgac aaggaaatcg tgactatgaa
      781 acaaattatt cagtcattca aaacaggaga aatctggttg gcggacgtgc ctgtacccat
      841 actcaaaccg gaaggcgttg tggttgaaac acatcattcg tttatttcat caggtaccga
      901 aaaaatgttg gtgtcgtttg cccagaaaaa tttattgggc aaggcctgtg ccatgcctga
      961 tcaggtcaaa aaagtgattc aaaaaattaa gactgaggga ttgtttgaaa cctgggaaaa
     1021 ggtacaggcc aaactcgatc agcctattcc tttgggctat tcgtgtgctg gtgtggtggt
     1081 ggaggttgga caaggggtca aggggttggt gaatggtgat cgtgtggcct gcgcaggggc
     1141 aggttatgcg aaccatgcgg agttcaatta tgtaccaaaa aatctgctgg tcaaaattcc
     1201 cgacaaggtt tcttttaggg atgcgtcctg tgcaacggtg ggtgcgattg ccctgcaggg
     1261 tgtcaggcaa tgtgatgtga gggtaggtga aactgtttgt gtgatgggat tgggtttgct
     1321 tgggcttttt gctgtacaaa ttttgaaagc cagtggtgcc aaagtggtgg gttttgaccc
     1381 tgatgcacga cgctgttcat tggcgcaaga gcttggggct gattctgtgg ttaaggacaa
     1441 tcttgtggcc acatgcgatg ctttttcaaa gggaaatggt gttgatggcg ttctcatcac
     1501 agcggcaacg aattccaatg agcctgttga gctggcaggc cagattgcgc gtcataaggg
     1561 caaagtggtg gtgacgggca tggttggaat gaacataccc agggatgtgt tttacaaaaa
     1621 ggaactggat ttcaaactga gcatgtctta cgggcccgga cgctatgata ccaattatga
     1681 agaaaagggg catgattatc cttatggcta tgtgcgttgg actgaacaac gaaacattga
     1741 gttctttttg gatttgattg ccgatggcca tgtgactcct tccaagatga tcacacatga
     1801 gtatgatatt gccgaaggac tcaaggccta cgatttgttg ttgggcaggg aaaaaagtga
     1861 gtatttgggt attgtgattg gttatggcaa atctgacgga acgacgagag tcccagaacg
     1921 caaagtgatt tgtggacaga accatcaggt aaccgattgt gtcgtgggta tgattggcat
     1981 gggcaatttt gcaaaagctg tgttgttgcc tgctttgcaa aaacaaaatc acatttcact
     2041 cagatgtctt tgtgccacaa caggaattga tatcacggca gtcgcaaaaa aaacaggatt
     2101 tgagtacgtc acgacggatt ctgccgaact ttttgctgac ccacgaatca ataccgttgt
     2161 gattgcgacc cgtcatgatt ctcatgccgg attggttgta aaggctctag aggcgggaaa
     2221 aaatgttttt gtagaaaaac cccttgcgtt gaacagggac gaattggaag ccatcaaaca
     2281 agctgttcaa attcactctg gaatattgac tgtagggttt aacaggcgtt tttcgccaca
     2341 tgccaagctt ttgagagact atttggcgaa aaaaacatcg cccgcggtgg taaattatcg
     2401 catcaatgcg gggcacattc cttatgactc ctgggttcat gatcccatta aggggggagg
     2461 gcgtatcatt ggtgaagttt gtcattttat tgattttgtc agttatattt tgtctgctat
     2521 gccgcggaca gtgcaagctt gctctgttgg tggtgataat gcagagcgac aggccgccga
     2581 caatgtttcg atcaatattg agtattcaga tggttctctg gctaatttta tttacacatc
     2641 gtttggatct cgtgaccatc ccaaagaaca gggcgagata tttgtggatg agtctgtgtg
     2701 tttaatgaag gattttgtga gtacagaatg ttttgggaaa ttgggccgaa aaaaactgaa
     2761 aggcaaacaa agcaaggggt ttcaggagga gtttttggct tttttctctg ctgtgatcaa
     2821 caagcaccaa caccctatcc ctcttgatga aatctttgtc acaacagagg cgactttttt
     2881 ggtaagtgaa gccatcaaaa ccagagaaac tttactaata acacaatgaa ttgttttggt
     2941 tacatgagca gatattttag aacactgaaa catttgaaag catctcaaat attttacagg
     3001 ctatattatg ctgtgatgcc attatgtcca aagaagatat ttattatttc atcgtgttct
     3061 ctaaaaccgg agttgtcttt cccagtggct aatcgcaaac attatcttgg cagtggagca
     3121 tttcttttca acaacaaaca aaatactttg gtgggtcgtg attgggattt tgtaaaaaat
     3181 gacaagctct ggagttatcg tttgcactat caggattggt tactggagga cgattgtccc
     3241 gttgacgatt gggtaaacaa atggattgat gacaactctc catttaaggg taatggctgg
     3301 gaggcttatc ccatctcact ccgtatggtc acatggatca agttgttggc tcaagggcga
     3361 ctctcagact ctgccaagat acggttgtcg ctggcacagc aggcatccgt tctttatgca
     3421 cgacgagagt accatctttt gggcaatcac ttattggaaa atcttttcgc tttgtatttg
     3481 gtgagccacg tctgttgcaa aacatcacaa caacaaatta tttatgatca gttggtcaaa
     3541 gaaatcgatg cacagttttt ggctgatggc gggcattatg aattgtcgcc aatgtatcat
     3601 gccattttgc tggaacgcat tcttgatatc ctcaatttga cccgcaaccg tcccctttcg
     3661 caaaacgttt atggcaagtt gattgatgtg gcacataagg gtttgaattg gttgt
//
LOCUS       DATDUN010000774         1702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_240939, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000774 DATDUN010000000
VERSION     DATDUN010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240939"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1309)
                     /locus_tag="VJC18_10810"
     CDS             complement(185..1309)
                     /locus_tag="VJC18_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HLD45913.1"
                     /translation="MKKIGRKVLGRVRGTAQFTAVPSKNELPSEYGKTLAELKSVLRL
                     ARVKTVLAANVSMVLAYWDMGRTILLRQKSSGWGAKIIDRLSADLHEAFPEMQGLSPR
                     NLKYMRAFAAAWPNRAIVQRIVAQLPWRHNIALIERLDNTKTRMWYAQQTIQQGWSQP
                     ILCLQIEQNLHERQGRAITNFPTTLPPVDSDMAAQIFKDPYLFDFVGTADPRRERELE
                     QALVEHIQKFLLELGAGFAFVGRQVHLEVGDKDFYLDLLFYHLQLRAYVVIELKATHF
                     DSSFVGQMNLYLSAVDDLLRHQDDKPNIGLLLCRDKNKLVAEYALRDVHKPIGVANWT
                     TKLMKRLPSNLRPSLPTVQDIEKSLKEIPKKKKKEGGSSH"
BASE COUNT          446 a          386 c          397 g          473 t
ORIGIN      
        1 cacccgggtc gttcactatt ggatgaccga agaatagacc ggaaggtcct ggcccgcccg
       61 tttctcgtaa ccacagtaat ggtgagcgaa atgagcgggc cagttttttt tcgataccct
      121 tccttattat tgcaacaaac agatcacgcc taacggcagt cgacaaaaaa tttaagtttg
      181 cttctcagtg gcttgatccc ccctcttttt tctttttctt cggaatttcc ttcaaagact
      241 tttctatgtc ttgtactgtg ggtaaactgg ggcgcaggtt ggagggtagg cgcttcatga
      301 gtttggttgt ccaattggct acgccgatgg gtttgtgaac gtcacgcagg gcatattcgg
      361 caaccagttt gtttttgtct ctgcacaaca agagtccaat gttgggtttg tcgtcttgat
      421 gtctgagaag atcatcgaca gctgacaaat agagattcat ttgtccaaca aacgaagaat
      481 caaaatgagt ggccttgagt tcaatgacca cataagctcg tagttggaga tgataaaaga
      541 ggaggtccaa ataaaaatcc ttatcaccca cctccaaatg cacctgtctg ccaacaaagg
      601 caaagccagc acccaactcc aacaaaaact tttgaatatg ctccactaac gcctgttcca
      661 actcgcgctc gcgacgagga tcggccgtac ccacaaagtc aaacaggtag ggatctttga
      721 aaatctgcgc agccatgtct gaatcgacag gtggtaaagt ggtaggaaaa tttgtgattg
      781 ccctgccttg tcgctcgtgc agattttgtt cgatctgaag gcaaagaatg ggttgtgacc
      841 agccttgctg aatggtttgt tgggcatacc acattcttgt tttggtattg tcaagacgct
      901 caattaaggc aatgttgtgc cgccacggca attgtgcaac gatccgttgc acaattgccc
      961 gatttggcca agcggcggcg aaagctctca tgtacttgag attacggggg gatagaccct
     1021 gcatttcggg aaatgcctca tgtaaatcgg ccgacaggcg atcaattatc ttggcacccc
     1081 atccagagga cttttggcgc aataaaatgg tgcgccccat gtcccaatat gcaagaacca
     1141 tagagacatt agccgccaaa actgttttga cacgagctag acgcaggaca gattttagtt
     1201 ctgccaatgt tttaccatac tcggagggga gttcattctt gctgggtacg gccgtgaatt
     1261 gggcagttcc cctgactctg cccaaaactt ttcgtccaat ctttttcata gacactgact
     1321 acgacagttt ttggaacaga tcgagaaaaa tggtgattca aaattcacta tcatacaatt
     1381 ctgctgtcat ggtttttttt ggtgaagggt tatttgacgg taaataatgt aagcgatgct
     1441 gttccaaaaa aaatagctta tgagaccagc tttttggctg agacttcaag tgctctggca
     1501 atcttttcta gcgattccaa ggtcaagttg acctgtccag attcgatacg ctggtagtgt
     1561 ttgcaacccc cttcccgtac tctttagagt acgaaatctg catttttgag tgcacgggat
     1621 tcaaaacaca accgataatc aggttgaaag ttgacttggt tagggcgcac agcttcaaat
     1681 atgagatcgt tcatatctgc cg
//
LOCUS       DATDUN010000775         1439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_203156, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000775 DATDUN010000000
VERSION     DATDUN010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203156"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VJC18_10815"
     CDS             <1..736
                     /locus_tag="VJC18_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HLD45914.1"
                     /translation="FKIVTFIELFKNMLKLSLVIYIAYITINKYMQDILLSSQVTMEK
                     AVIITGQIVYSFFSKVAAVFIVVALIDMGVQRWNYNKRHKMSKDEVKREYKQDEGDPQ
                     IKGERRRLHRDMVFGDPRQAVKKADAVVSNPVHVAVAIGYNRDEMAAPEVLIKGQRRY
                     AEMILELAREENIPIIRNIPLAWSLLQVEEGDAIPEDLFEPVAEVLSMVYEMKQNQQS
                     PVPQADVVTTNSAGASKPTTFNPLGQ"
     gene            953..>1439
                     /locus_tag="VJC18_10820"
     CDS             953..>1439
                     /locus_tag="VJC18_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45915.1"
                     /translation="MKNMTKSVLLLCVLVSTHLVACSSGPDKNKTATSPSQAQTVPAS
                     SGPSQLDAVEFKDKRLVAGFFSYQHKNALPMLTAISKLLEEQIKYNFRILAISIDYND
                     IAGLKKFVAEHQLKSPVLVDSADLRLVEQYKVENEVAVVGLDARHQLAFGIKDYVFAD
                     KP"
BASE COUNT          381 a          326 c          363 g          369 t
ORIGIN      
        1 gttcaagatt gtgaccttca tcgagctctt caaaaacatg ctcaagttgt cgctggtgat
       61 ttacatcgcg tacatcacga tcaacaagta catgcaggat attctcctgt cctctcaagt
      121 gaccatggaa aaagcggtga tcatcacagg gcagatcgtc tacagtttct tttccaaggt
      181 ggcggcagtt ttcattgtgg tggccctcat cgacatgggc gtgcaacgat ggaactataa
      241 taaacgtcac aagatgagca aggacgaggt caagcgtgag tacaagcagg acgaaggcga
      301 cccgcagatc aagggagaaa gacgccggtt gcatcgtgat atggtgtttg gtgatcctcg
      361 ccaggcggtc aaaaaagctg atgccgtggt gagtaatccc gtgcatgtgg ccgtcgcgat
      421 tggttacaat cgcgacgaga tggcggcgcc cgaggtgttg atcaagggac agcgccgtta
      481 tgccgaaatg attctggagt tggcccggga ggaaaacatt cccatcatcc gcaacatccc
      541 tctggcgtgg tccctgttgc aggttgagga aggagacgcg attcccgagg atttatttga
      601 gccagtggca gaagtcctca gtatggtgta cgagatgaaa caaaaccagc agagccctgt
      661 gccccaagct gatgtggtga ccacaaattc tgcgggggct tccaaaccga caacatttaa
      721 tccgttgggt caatgaagaa atgaaacaac gtggatcgtg aatcgacaat cgtgaatcga
      781 aagcaagtat cattccagtt acagcttata tccgtgttct gggccttttt gtgtttatta
      841 tttgtagaat gacttattct tctgtttctt ctatccacta ttcacgatcc acgattcacg
      901 ctattttatt cccaatttgc atattcagac aatattggtt agatattccc ccatgaaaaa
      961 catgacgaaa tccgttctgt tgttgtgtgt tttggtgtcc acgcatttgg tggcgtgttc
     1021 atctggtccg gacaaaaaca agacagcgac atcaccttca caggcacaga ctgtaccagc
     1081 ttcttcaggt ccctctcaac tcgacgccgt ggaattcaag gataaaaggc tggtggcggg
     1141 atttttcagt taccagcaca agaacgcttt gcccatgctg acggccattt caaaactcct
     1201 ggaagagcag attaaatata attttagaat tctcgcgatc agcattgatt acaacgatat
     1261 agcgggtctg aaaaaatttg tggcagagca ccagctgaaa tcacctgttc tggtcgatag
     1321 tgccgatttg aggctggttg aacaatacaa agtcgaaaac gaggttgctg tggtgggtct
     1381 cgacgcccgt caccagttgg cctttgggat caaggattac gtatttgccg acaaaccaa
//
LOCUS       DATDUN010000776         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_572396, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000776 DATDUN010000000
VERSION     DATDUN010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572396"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2011)
                     /locus_tag="VJC18_10825"
     CDS             complement(<1..2011)
                     /locus_tag="VJC18_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45916.1"
                     /translation="MRTVTKTDDSESRWARFEVPPESLWRQQRDPQIIADSPLDGFDL
                     YLARLGLLALPDDNPSSWIFGRSSSGLFLPSGHINPHQALRFFLDNKSAEIDLEPSSL
                     IERPSVGGIDIEHFLNTNRFGVVPIRLTSNQEPLGAFFSAQKGALYYLETLESATQAL
                     DSGAISASERIVLSNEFKQILSAAYDSFIKAYECWWRLASEEGSLSQMRTESLVNKTI
                     KTMSSGDKPPWVVKLTLPFSKWNSLLGEISANQIKWYLLSALKREMAGGEALGKVCFI
                     DGTGISILFDADEFSPTQITTLMDRCNELLISELFCRISQGRLSKQIREGGRGFEVLN
                     DDGRWIPLSLLFSNDVYAGAAQVNVKGLSQNDGYQTEDAVRRAVHKTEILARARAEAA
                     QKNSQSQGTMTSSQNRFDWRSTKKDLSSEWGKGYFPEGVIPTKEDLCWAVFTAFYRFG
                     FMDSPSAESLLQQKKKGLLQILRLADLASTKLKQRKGGEEGAVSSLGFMQSLFDDLAS
                     AHDANDQNFQKFLENTSYNTDVFFDVIHSFIVGSAKEQMALHSFLSLASARLVSHEAG
                     DLAGNKGALTTLGAVMQTESSKPLPKATIPDLTHALSRVPWMLTRGMSEALMDARFPG
                     KIKTNAFERVIANHFQGDFHLFVFDYDNLASFLKNPENRKIPDQPY"
     gene            complement(2228..>2499)
                     /locus_tag="VJC18_10830"
     CDS             complement(2228..>2499)
                     /locus_tag="VJC18_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45917.1"
                     /translation="EPAKAEGQAVPAQAQEALMQTPGDHNDTQTVLLETALASVVLQD
                     RPSISPKEKQPASTLRTSSVFKATKVLNDYTEFDDQYIINDNGGL"
BASE COUNT          615 a          596 c          626 g          662 t
ORIGIN      
        1 cgtagggttg atcaggaatc tttctgtttt ccggattttt tagaaaactg gcaagattat
       61 cgtaatcaaa cacgaacaga tgaaaatcac cttgaaagtg attggctatg acgcgctcaa
      121 aggcattggt ttttatttta ccaggaaagc gtgcgtccat cagggcttcg gacatgcccc
      181 gtgttagcat ccagggcaca cgcgaaaggg cgtgcgtcag gtcagggatg gttgcttttg
      241 ggagcggttt gctggactct gtctgcatca cagcgccaag agttgtcagc gcccctttat
      301 tgccagccaa atcgcccgct tcgtgcgaaa ccagacgggc tgatgccaaa gaaagaaaag
      361 agtgaagggc catttgttct ttggctgaac cgacgatgaa gctgtggatc acgtcaaaga
      421 aaacatctgt gttgtatgac gtgttttcca agaacttttg aaaattctga tcattggcgt
      481 catgagcgct ggccaagtca tcaaacaggg attgcataaa cccaagcgat gaaacggcgc
      541 cctcctctcc tccttttctt tgtttgagtt tggtgctggc caaatccgca agccggagaa
      601 tttgcaagag cccctttttt ttctgctgta acaggctttc tgctgagggg ctgtccataa
      661 atccaaaacg gtaaaaggct gtgaaaactg cccagcaaag atcttctttt gtgggaatga
      721 caccctcagg gaaatagccc tttccccatt cggaagagag gtcttttttt gtcgaccgcc
      781 agtcaaaacg attttgcgaa gacgtcatgg tcccttggct ttgagaattt ttttgtgccg
      841 cctccgcccg agccctggca agaatctctg tcttgtgaac ggcgcgtctt accgcatctt
      901 ctgtctgata accatcgttt tgggaaagtc ctttgacatt gacctgtgcc gcccccgcgt
      961 acacatcgtt tgaaaacaaa agggacagag gaatccagcg cccgtcatca ttcaagactt
     1021 cgaacccccg ccctccttct ctgatttgtt tagagaggcg tccttgagag atgcggcaaa
     1081 aaagctcgct aatcaaaagc tcgttgcagc gatccatcag cgtcgttatt tgtgtgggag
     1141 aaaattcatc ggcgtcaaaa aggatagaga tgcccgtgcc atcgataaaa caaactttgc
     1201 caagagcctc cccgcccgcc atttcccgct ttaaggcgct gagcagatac catttgatct
     1261 gatttgctga gatttcaccc aaaagagaat tccacttgga aaaaggtagc gtcaacttga
     1321 cgacccaggg tggtttgtcg cccgaactca tcgtcttgat tgttttgttc acgagtgatt
     1381 cggttctcat ttgtgacaga gacccttctt cggaggcgag tcgccaccaa cactcatatg
     1441 cctttatgaa ggaatcatac gccgcggaca gaatttgttt aaattcgttc gagagcacga
     1501 ttcgttcact ggcgctgatg gcccctgagt ctagggcctg cgtcgcagac tccaaggtct
     1561 ccagatagta tagggcccct ttttgcgcgg agaaaaaggc accgagaggc tcttggtttg
     1621 aggtgagcct gatggggaca acgccaaaac gatttgtgtt caagaaatgc tcaatatcga
     1681 ttcccccaac cgagggcctc tcaattaacg aagagggttc cagatcaatt tctgctgatt
     1741 tgttatccaa aaaaaaccta agggcttgat ggggatttat gtgacctgag ggcaggaaca
     1801 gccccgagga acttctgcca aatatccacg aagaagggtt gtcatctggc agggccaaga
     1861 ggccaaggcg ggccaaatac aaatcaaaac catccaaggg gctatcagca atgatttggg
     1921 ggtctctttg ctggcgccaa agggattctg gagggacctc aaaacgggcc caacgacttt
     1981 ctgaatcatc agtttttgtt acggtccgca taggtgttgc tgtccatcat aaaggccaat
     2041 gcaatcgccc agtgatttcc ttgcctcgtt gtgtttctga agttttgcac acacacgaca
     2101 aaacctggat atcgtttagg gcatctctaa aaactatctt ctaccatagt ttttagagat
     2161 gccctttcct ttaattatcg gcagtttctg tgaagagttg tgtgttctcg caggaatcga
     2221 aaaaaaccta taacccgccg ttatcattga tgatatattg atcatcaaac tcggtataat
     2281 cgttgaggac ctttgtggct ttaaaaaccg aactggtacg gagggtggac gcgggctgtt
     2341 tttctttagg gctaatggag ggtctgtctt gcaagacgac agaggctagg gcggtttcta
     2401 acaggaccgt ctgtgtatca ttatggtccc ctggtgtttg catgagcgct tcctgcgctt
     2461 gtgcaggaac agcctgcccc tcggctttgg cgggttctg
//
LOCUS       DATDUN010000777         3367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_203394, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000777 DATDUN010000000
VERSION     DATDUN010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203394"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VJC18_10835"
     CDS             <1..773
                     /locus_tag="VJC18_10835"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLD45918.1"
                     /translation="APFPSCGFNIMRRSGLLPLVFPDVHQMIGVLQPKKENEDVYDHT
                     MRVLDAMRSSPDIEKAGNPTLLFAALFHDAGKPKTRRENPQTGHVSFYNHNVVSAGIA
                     GRWLKQFKASTIGIDTHLVCHLIKNHMFETKHFLNNEKSLRRFINKVGVEPIFDLLDL
                     RLADKMGGRFPKKLFGILSLRDKVREEINKAPPFTPKDLAINGHDIMTLGYQPGPIFG
                     EIQRFLMDKVLDDPELNTKEILLRLITDNKDQLLQLLQ"
     gene            882..1346
                     /locus_tag="VJC18_10840"
     CDS             882..1346
                     /locus_tag="VJC18_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45919.1"
                     /translation="MKTNTDQSLVWELKGDKLLSSQKNKKAFEAYQKALELNPARLSL
                     YDKLLALHDQFADNWDDSDFAYNMSLTMKKQELINPVYKRIHARLDVHFKTVAELIKK
                     MLSAPTPEAETDCVERIVSCGSLALYPLIDYLLTFKEVLRHQKNKPQTKTDK"
     gene            1343..1987
                     /gene="coaE"
                     /locus_tag="VJC18_10845"
     CDS             1343..1987
                     /gene="coaE"
                     /locus_tag="VJC18_10845"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HLD45920.1"
                     /translation="MTISPSQKRIRKPTGTEIIGITGGLATGKSTVSALLRRSGYFVI
                     DTDAIAHDVVKPHQKAFRQIVEAFGQAIISKDGTIDRDALAQLVFINKTKRKELEAIV
                     HPEVKKAVKNIIDILKKQKPRLIFLEVPLLFETDFHKLCHQTVCVTSSQSHQLERART
                     KRHMSRSQALARIRAQMSLSKKCKRADFVIENNAGKKELTQKIKIWLKALSCSW"
     gene            2109..>3367
                     /locus_tag="VJC18_10850"
     CDS             2109..>3367
                     /locus_tag="VJC18_10850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD45921.1"
                     /translation="MNHSIAYRQRRFINWFSIGLAYAFLYMARYNLTVTKNALGDLMS
                     KVEFGNIFGAGALVYGFAFIINGPLSDRYGGRRVMLIATMGAAIANFLMGLVLFGITN
                     WHWSMPIYTTFMVLYAINMYFQSFGALAIVTVKAPWFHVSERGTFSTIFGIMISLGLY
                     FAFDWGYAIMDATRAIVDTTKLGLIAKIVIGVLGTGNAGVDQNWWLFYIPSFFLTVFW
                     LVMLLVLRNSPGEAGHANFDTGEDSITKEGEKTSTVEVFKRIFRHPVLIVVCGIEFCS
                     GFLRNGVMQWYPIFAKEIGIYNSFYITKNWGLMMLIAGITGGVLTGWASDKFFNSKRA
                     PMCGISYGLMLLAALWMIFTLTGNSWHVGIMVLIISMAVIGVHGIMSGTATMDFGGAK
                     NTGTATGIVDGIVYLGVAVEAFVIGHIT"
BASE COUNT          873 a          914 c          764 g          816 t
ORIGIN      
        1 aagcgccctt tccctcctgc gggttcaata tcatgagacg ctccggtctg ctccctcttg
       61 tttttccaga cgtgcatcag atgatcggcg tcctgcaacc caaaaaagag aacgaggacg
      121 tatacgacca caccatgcgc gtgttagacg ccatgcgctc atcaccagac atcgaaaagg
      181 caggcaaccc cacgctcctg ttcgcggccc tgtttcacga tgccggcaaa cccaaaacac
      241 gccgtgaaaa cccgcaaacc ggacacgtga gtttttacaa tcacaacgtg gtctcagccg
      301 ggatcgctgg ccgctggctc aaacaattca aggcatccac cattggtatc gacacgcacc
      361 tggtctgtca cctgatcaaa aaccacatgt ttgagaccaa acattttttg aacaacgaaa
      421 aatcgcttcg gcgcttcatc aacaaggtcg gcgtggaacc catttttgat ttattggatc
      481 tgcggctggc cgacaaaatg ggtggccgct ttcccaaaaa actcttcggc attttgagct
      541 tgcgggacaa ggtccgggag gaaatcaaca aggccccccc gttcaccccc aaggatctgg
      601 ccatcaatgg acacgacatc atgaccctgg gctatcaacc tggcccgatc ttcggagaga
      661 tccagcgatt tttgatggac aaggtgttgg acgatccgga actgaatacg aaagaaatct
      721 tgctaaggtt gatcactgat aataaggatc aattgctgca actacttcaa tgaacaacag
      781 acaattggca attgatgtag ttgatcgttg agacaattga agcagttgat ttgccttacg
      841 ccttaagcct tgggccttat gcccttcgta aacacgtaat tatgaaaaca aacaccgacc
      901 aatccctggt ttgggaactc aagggggaca aactgttgtc ctcccagaaa aacaaaaaag
      961 ccttcgaggc ctatcaaaag gcactggaac tcaatcccgc gcgactgagt ctctacgata
     1021 agctcctcgc cctgcacgat cagtttgccg acaactggga cgactctgat ttcgcgtaca
     1081 acatgtcgct caccatgaaa aaacaggaac tcatcaatcc tgtgtataag cgcattcacg
     1141 ccagactcga tgtccacttc aaaacagtag ccgaactcat caaaaaaatg ctctcagcac
     1201 ccacccctga ggccgaaaca gactgcgtgg aacgcatcgt gtcctgcggg agcctggccc
     1261 tctacccgct catcgattac ctactcacct tcaaggaagt cttgcggcac caaaaaaaca
     1321 aaccacaaac caaaacagac aaatgacaat ttccccatcg caaaaaagaa tccgcaaacc
     1381 aactggcact gaaatcattg gcatcacggg cggactggca accggcaaaa gcactgtttc
     1441 tgcccttctc agacggtctg gttattttgt gatcgacacc gacgccattg cccacgacgt
     1501 ggtcaaacca catcaaaaag ccttcagaca gatcgtggaa gcctttggac aagccatcat
     1561 cagcaaagac ggcaccattg atcgtgacgc gttggcccaa ctcgttttta taaacaaaac
     1621 caaacgaaag gaacttgagg ccatcgtaca ccccgaggtg aaaaaagcgg tcaaaaatat
     1681 aatcgacatt ctcaaaaaac aaaaaccacg cctcattttt ttggaagtgc ccctgctgtt
     1741 cgaaaccgat tttcacaaac tctgccacca gaccgtttgc gtcacctctt ctcaatccca
     1801 ccagcttgaa cgtgcccgca caaagaggca catgagtcgc tcacaggccc tggcccgtat
     1861 ccgcgcccag atgagtcttt cgaaaaaatg caagcgagcc gatttcgtca tcgagaataa
     1921 tgcgggcaaa aaagagctga ctcaaaaaat caaaatctgg ctcaaagccc tctcttgttc
     1981 atggtaacaa tttgatattg tttgcttaat tttttatcac ttctcgccac cattctcttg
     2041 caatcaatta acatctcttt tatggggcaa tgtccttgtt gtaaaaaatt tgaacaaagg
     2101 agctccacat gaatcacagt attgcctacc gtcaacgccg tttcatcaac tggttttcga
     2161 tcggactcgc ctacgccttt ctctatatgg ctcgctacaa cctcaccgtc accaaaaacg
     2221 ccttgggtga tttgatgagc aaggttgagt tcggtaatat cttcggtgcg ggcgcgctgg
     2281 tctacggatt cgcctttatc atcaacgggc cgctgtctga ccgctacggt ggcaggcgtg
     2341 tgatgctcat cgccaccatg ggagccgcga tcgccaactt cctcatgggt ctggtcctgt
     2401 ttggcatcac caactggcat tggtccatgc ccatttacac cacgttcatg gtcctctacg
     2461 ccatcaacat gtattttcaa tcctttggtg ctttggcgat cgtcacggtc aaggccccct
     2521 ggttccacgt ctccgagaga gggaccttct ctaccatttt tggaatcatg atctcactgg
     2581 gtctttattt cgccttcgac tggggttatg cgatcatgga tgccacgcgg gccatagtgg
     2641 acacaaccaa gttaggcctg attgcaaaaa ttgtcattgg cgtgttggga actggcaatg
     2701 ccggtgtgga ccagaactgg tggttgtttt acatcccttc ttttttcctg accgtcttct
     2761 ggctcgtcat gctcctggtt ttgcgaaact cacctggtga ggcgggacat gccaattttg
     2821 ataccggcga ggattccatc accaaagaag gggaaaagac ctctacagtt gaggttttca
     2881 aacgcatttt tagacaccct gtgctcatcg ttgtttgtgg catcgagttc tgcagtggtt
     2941 ttttgcgcaa tggcgtgatg cagtggtatc ctattttcgc taaagaaatc ggcatttata
     3001 attcctttta catcacaaaa aactggggcc tcatgatgct catagcggga atcacgggtg
     3061 gggtcttaac agggtgggcc tccgataaat ttttcaattc taaacgagcc cccatgtgcg
     3121 gcatctctta cggactgatg ttgctggccg cactctggat gatcttcacg ctcacgggaa
     3181 acagctggca cgtgggaata atggtgctca tcatttcgat ggcggtcatc ggcgtgcatg
     3241 gcatcatgtc ggggacagcc acgatggatt ttggcggcgc caaaaatacc ggcacggcca
     3301 cagggattgt ggacggcatc gtttatctcg gcgttgccgt tgaggccttt gtgatcgggc
     3361 acatcac
//
LOCUS       DATDUN010000778         1354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_449051, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000778 DATDUN010000000
VERSION     DATDUN010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449051"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..>1354
                     /locus_tag="VJC18_10855"
     CDS             120..>1354
                     /locus_tag="VJC18_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HLD45922.1"
                     /translation="MMTVIIFILALSILIFVHEWGHFIVAKKSGIRVDVFCIGFGPKL
                     FSFTWGGTEYRIAPFPFGGFVKIFGHEPMEEAEGDAVRAKEIAEHPQAFVSKPLWKRL
                     AVVFSGPLMNIILCLLLLPVVYMVGQYKPKIVFEKPVVVEVTHGSPAERAGLQKGDEI
                     TKFNDKTVTNWEDLMTQIMVHPEEEVSLAFLRAGQLRTVKTKPVIKKEGDQSMGYLGI
                     EYYEFYGNEPVVGEVQKETPADRAGIRSGDLIVAINGKPIQYWTEMMEVVRGSEGRSL
                     DVTVERAAVNGVDARERMIMVVKPEFSEKAKYWVMGIQKDIIKDGLVRVRHGFTDSVK
                     LGVRKFGSLTNMTLDVLYRLFTGQLSVKVLGGPLQIAKLTSSAAKSSFGDFLFILAFL
                     SLQLGILNLMPIPVLDGGH"
BASE COUNT          336 a          286 c          393 g          339 t
ORIGIN      
        1 caattcatcg atgtctgcct ccagcggcac ggtcccttcg ctgtgtctgc gttggaagat
       61 gttttggcgg cagacctgtg ggcgcgtgaa tttgtaaaaa accagatagg aaacaaaaca
      121 tgatgacagt gatcatattt attcttgccc tgagtatcct catttttgtg catgagtggg
      181 gtcattttat tgtggctaaa aaatcaggca ttcgggtgga tgttttttgc attgggtttg
      241 gtccgaagct tttcagcttt acctggggag ggaccgagta ccgcatcgcg ccctttccct
      301 ttggtggttt cgtcaaaata ttcggacacg agcccatgga agaggccgag ggtgatgccg
      361 tgagggccaa ggagatcgct gagcatcccc aggcctttgt gtccaaaccc ctgtggaaga
      421 ggttggccgt ggtgttctct ggccccctga tgaatatcat tttgtgttta ttgctgttgc
      481 ctgtggtgta catggtggga cagtacaaac caaaaattgt ttttgaaaaa ccggtcgtgg
      541 ttgaggtcac tcacgggtct cccgcagagc gggctggttt gcaaaaaggc gacgagatca
      601 ccaaattcaa cgataaaaca gtcaccaact gggaggacct gatgacacaa atcatggtcc
      661 accccgagga agaggtgtca ctcgcgttcc tcagagcggg tcaattaagg actgtcaaga
      721 caaagccagt gatcaaaaaa gagggtgatc aatccatggg gtatctgggg atagaatact
      781 atgaatttta tggtaacgaa ccagtggtcg gagaggtgca gaaggaaaca cccgcggatc
      841 gtgcgggaat caggtccggg gacctgattg tggccatcaa cggaaagcca atacagtact
      901 ggaccgaaat gatggaggtg gtgaggggga gcgaaggacg ttcgcttgat gtgactgtgg
      961 aaagagctgc cgtgaacgga gtcgatgcca gagaaagaat gatcatggtg gtcaaacccg
     1021 aattttcgga gaaagctaaa tactgggtga tggggattca gaaggacatc atcaaggatg
     1081 gtttggttag agtccgtcac ggttttaccg attcagtcaa attgggtgtg agaaaattcg
     1141 gaagtctcac caacatgaca ctggatgttc tttaccggct gtttacaggg cagctatcgg
     1201 taaaggtcct gggagggcct ttgcagatcg ccaaactcac ttcatcggca gccaaatcga
     1261 gttttgggga ttttttgttc atcctggcct ttttgtccct gcaactcggc atcttgaatc
     1321 tcatgcccat cccggtgctg gacgggggac acat
//
LOCUS       DATDUN010000779         8352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_617075, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000779 DATDUN010000000
VERSION     DATDUN010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617075"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VJC18_10860"
     CDS             <1..237
                     /locus_tag="VJC18_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45923.1"
                     /translation="EKKKLSRPRVALTTEGIFGGTLELEIGEKLTTHIEHMWGQSKGH
                     RHESIEREVRSQVTKWMKTHYDLRPEVIVLEHHI"
     gene            234..1076
                     /locus_tag="VJC18_10865"
     CDS             234..1076
                     /locus_tag="VJC18_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45924.1"
                     /translation="MKIRASLKNCFLFILIVLCLWTSDLLAQAMPIEKMSLTIEMQHV
                     GEHEDILTPKTVKFAQTDKALRLFWYSLVKKLDQEAYFHTYRVEASRGIVETDNFAGK
                     RALILPALWGDGYVRSETALPLWVDPEFLSLKGKKQLPFNAGLLNVNKQLLKAAPDEV
                     FEKVNYLQNLYDQYVSGFTVRSDIKLKKVLAKELDDFIRNFFFVVKLSESKVTIAVNR
                     VNEEYPVVWLGNDYYELAVLNDPLNPLVLNFKIHEDKTPKVLQEQFGEIAKLVNFSVK
                     EITY"
     gene            1073..3211
                     /gene="recG"
                     /locus_tag="VJC18_10870"
     CDS             1073..3211
                     /gene="recG"
                     /locus_tag="VJC18_10870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HLD45925.1"
                     /translation="MSLSGPHKLVPATPIQYLKGVGPRLAIVFAKKAVTTAWDMLHYF
                     PVRYVDRRQLSSIAQLQIGQKESFLAKIIEYHVTTIRGRRRKLMEILVGDGQDHAIIT
                     LFQFNENYLKKRFPPGSQVLFFGEVRVYRGLKNLVHPDMEVWDDDDAGPGAGRLVPIY
                     PLTEGLYQKTVRNIISKNFEALLELVIDEPRSVRDGDPVPLSLVEALRYLHQPPVDAD
                     VALLNERKTVWHQRVIYDEFFYLQLALLARRRVQARQHAFVVGPRDIALAHQCAARLP
                     FELTGDQKQVLHEIIADFQTARPMNRMLQGDVGSGKTIVAFLSALPIMSAGYQAALMV
                     PTEILAEQHYHNLNKLAQPLNIEVGLLTGAIKGKQRKQIHQDLQTGALQLLIGTHALI
                     TPDVVFNKLGYVIIDEQHRFGVVQRALLKQKTSVYCGQEILPHLLIMTATPIPRTLSM
                     CVYGDLDMSLIREMPKGRLPVITKLYREAQLEQMHQFIAQELRKGRQAYFVYPLIEES
                     EKSDLKDATLMHQKLQKVFSDRTVGLIHGKLKAAEKESIMGQFKAGRLHVLVSTTVIE
                     VGVDVPNATIMVVEHAERFGLSQLHQLRGRVGRGAERSYCFLVAQYAQSEESRYRLRI
                     MEQTTNGFEIAEEDLRIRGPGEFLGTRQSGLPDFRLAEIVRDQHLLVAAQKRAALILH
                     EDPNLNLPKNGLIKNIMLERWGNRLELGTV"
     gene            3309..4526
                     /locus_tag="VJC18_10875"
     CDS             3309..4526
                     /locus_tag="VJC18_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45926.1"
                     /translation="MSHSISNPYKPGAGHMPPVLAGRENEITEFRMILKQSTILENLV
                     LTGLRGVGKTVLLETFKPLAIHERWLWVGTDLSESAGVSEETMVIRLLTDLAVVTSQF
                     VIHKEEKHALGFLSKKHFLEETLNYTALMSLYNRFPGLVSDRLKAIMEVVWRVLSSHR
                     IQGVIFAYDEAQTLADHTKKDQYPLSMLLDVFQSVQRKNLPFMLLLTGLPTLYSKLVD
                     ARTFSERMFRIIVLDKLTKEQCHEAIVKPLQGSTERFSAESVNSIIEESDGYPYFIQF
                     ICKEAFDELMVKKAVGEKRTFVAINEITRKLDRDFFAGRWTKVTERQRDLLVLIAQLN
                     NCDKEFTVQEIVNKSTQNSKSPFSSSHANQLLSALTQSGLVYKNRHGRYSFAVPLLGR
                     FIRRLGENEDNVV"
     gene            4704..7133
                     /locus_tag="VJC18_10880"
     CDS             4704..7133
                     /locus_tag="VJC18_10880"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HLD45927.1"
                     /translation="MKTNSFSKSLSVAIGLFLVFFVFSIAHAQNTVSTQLEDRASPVS
                     TNTPNVDTTIDAFEEIEKFRESAAFTAQQIDQFSRWNNLKENLDYLTRNYLKHFSTYQ
                     KKKKLSGWSLNQASQLKLKAERFFGKAAQYKETHLEYLKDTETLIANLNAQKRSFKKM
                     HQDFRTDASLRTEADLLQKDIAKINQDITTIKQHRSAYTVFYDVHNDVYGSIDEFERS
                     LDRDIDYFKSSLFDKNEPPLYDADFFAQFTPHLFSETITIWSESISFNKNALWKKKAE
                     FIHLAALLAVLWLVISRLGKRVNSNLLKHPLLTSVILTTLTGGLYLRNANNVLIVFFW
                     VVLFTLLIFLVRKLFEKKGTRGEVITLLVIYGLIQILDTVGLAVCLYRLLLVGLGLYI
                     ATYCFLRSRRPNTENLKHAILLTMIAFFVGASIMEVTGYHLLTVLLIHGTIKSAFLIF
                     AIKYLRKYFIEILSALFLLPGLSRFQVIKNNHHFIERKITRLFHLFIGFVLLSGLLAI
                     WGVFDTFTLSLSGVWSWGMSLREHRITIGMIITASVIVYLIKAVTHFSRALLEQEWYP
                     RKNVSEGTGKAVNSIMTYGIWVVGLGLILSVLGFGLEKITIIAGALSVGIGFGLQNIV
                     NNFISGLVLLFERPIKVGDILVIDDEWGVVEKVGLRSTTIRNYSQAHVIIPNADFVSQ
                     KVINLTHTDAKYRIKVPISVAYGSDLTKVKQLLEALTRAHPKIAKQPEPLVVCRMLGE
                     SAVQFDLLAWLDNVSDSYIVTGDLLTEADRTLRAHGIGLPFPQRELHIKSVDEKVTPG
                     LPSRCGDDRFR"
     gene            complement(7130..>8352)
                     /locus_tag="VJC18_10885"
     CDS             complement(7130..>8352)
                     /locus_tag="VJC18_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLD45928.1"
                     /translation="EDDFPPFRKATLFSLFFIFLGLFGGMALSFFQVIFHQLSWDVSL
                     GYGLAFFLSFCLISYPGTIMISTHVIRKNLFNKGILVKKLSSLPKLADLDLLYLNKTG
                     TLTQGTFVYAQHYLEQGSNQGQLLSTFFSLAAASHHPLAEALETHPWYHEITRHPVEE
                     LKEYPGLGVCGYINPSKGDHRFFAALGNLRFLKRMQMQVSRDMKNKIDDLEALGETVL
                     LGGYDRFVRGVMSFADVLRTDIHSTLKEIQELQIEPAIITGDAEDDVSHLITELKLTK
                     VYARCTPEEKAAKIEKDIADGKIVGFVGSQDYSLPFEKAHVSVSVDTGTQIIDQTADV
                     LILGSRIRLLSWLLQQTQQLFSRNRTRLTTSVILSLLVATTSVLGITSAEFVVTFICL
                     INILTLRSSAADAS"
BASE COUNT         2134 a         1957 c         2145 g         2116 t
ORIGIN      
        1 gaaaagaaaa aattgtcccg tccgcgcgtg gccctcacga ccgagggaat ttttggaggg
       61 acccttgagc tggagattgg tgagaagctg acgacccaca tagagcacat gtggggtcag
      121 tcgaagggcc accgacacga atccattgag cgcgaagtcc ggtcgcaggt gaccaagtgg
      181 atgaagactc actacgattt gcggcccgaa gtgattgtac tggagcatca catatgaaga
      241 tcagagcaag cctgaagaat tgttttctgt ttatattgat tgtgttgtgt ctgtggacct
      301 ctgacctgct ggctcaggcc atgccgatcg aaaagatgtc cctgacgatc gagatgcagc
      361 acgtgggaga gcacgaagac atcctcactc cgaaaaccgt caaatttgcg caaaccgata
      421 aagccctgcg gctgttctgg tacagtctgg tcaaaaagct tgatcaggag gcctactttc
      481 acacctaccg cgtagaagcg agtcgtggta ttgtggagac agataatttc gcggggaaga
      541 gggcattgat tctgcctgcc ctgtggggag atggttatgt tcggagtgag acggccttgc
      601 ctctgtgggt cgacccagag tttctgtcgc tcaaggggaa aaaacaattg cccttcaacg
      661 cgggtttgct caatgtcaat aagcagttgc tcaaggccgc gccggatgag gtgttcgaaa
      721 aggtcaatta cctgcagaat ctgtacgacc aatacgtgtc ggggttcacg gttcgttccg
      781 atataaaact caaaaaggtg ttggcgaagg aactggatga tttcatcaga aattttttct
      841 ttgtcgtcaa actcagcgag tccaaagtta ccattgccgt gaatcgtgtg aacgaagagt
      901 atcctgtggt gtggttgggc aacgattatt acgagttggc cgtattgaat gatcccttaa
      961 accccttggt gctcaatttt aaaattcatg aggacaagac ccccaaggtt ttgcaggaac
     1021 aatttggaga aatcgccaaa cttgttaact ttagcgtcaa ggaaatcact tattgagcct
     1081 gtcaggtcca cacaaactgg tcccagcaac ccccattcag tatttaaaag gtgtggggcc
     1141 gcgcttggcc attgtgttcg caaagaaagc cgttacgacg gcatgggaca tgctgcatta
     1201 tttccccgtc cgttatgtgg acaggcggca gttgagttcc atcgcgcaac tgcagattgg
     1261 tcagaaggaa agtttcctgg ccaaaatcat tgagtatcac gtgaccacga tccgtggtcg
     1321 gcgtcgcaaa ttgatggaaa tactggtggg cgatggtcag gatcacgcga tcatcacgtt
     1381 atttcagttc aacgaaaatt atctcaaaaa aagatttcct cccggttcac aggttttatt
     1441 ttttggtgag gtgcgtgttt atcgcggttt gaagaacctg gtgcaccccg atatggaagt
     1501 ttgggatgat gacgatgcgg gtccgggggc aggacgcctg gtgccgatct atcccctgac
     1561 ggaaggttta taccaaaaaa cagtccgcaa tatcatcagt aaaaactttg aggccctgct
     1621 cgagttggtg attgatgaac cccggagtgt gcgagacggc gaccctgttc ctctgtctct
     1681 cgttgaggcc ctgcgatact tgcatcaacc gcccgtagat gccgatgtgg ccttgctcaa
     1741 tgagcgcaag accgtttggc atcagcgtgt gatctatgac gaatttttct atctgcaact
     1801 ggccctcttg gccaggcggc gtgtgcaggc ccgccagcac gcgtttgtgg tcggtcccag
     1861 ggacattgct ttggcacacc agtgcgcggc acggttgccc tttgaactca cgggggacca
     1921 aaagcaggta ctgcacgaaa tcatcgcaga ctttcagaca gcacgtccca tgaaccgcat
     1981 gttgcagggg gacgtggggt ctggcaaaac catcgtggcc ttcttgtcgg ccctgccgat
     2041 catgtctgcc gggtatcagg cggctcttat ggttccgacc gaaattctgg cggaacaaca
     2101 ctatcacaac ctcaataaac tggcccagcc actgaacatc gaggtgggtt tgttgacggg
     2161 ggccatcaag ggaaaacagc gcaaacagat tcatcaagat ctgcaaacgg gggccctgca
     2221 gttgctcatc ggtacgcacg cactcatcac gccggacgtt gtgttcaaca aactgggtta
     2281 tgtgattatt gacgagcaac accgctttgg tgtggtacag agggccctct taaaacagaa
     2341 gacgagtgtg tattgtggac aggaaattct gcctcacctt ttgatcatga cggcgacacc
     2401 catcccgcgc acactctcga tgtgtgttta tggcgatctc gacatgagcc tcatccgcga
     2461 gatgcccaaa ggccggctcc cggtcatcac caaactgtat cgcgaggcac agcttgaaca
     2521 gatgcaccag ttcatcgcgc aggaactcag aaagggcagg caggcctatt ttgtgtatcc
     2581 cttgattgag gaaagtgaaa agtccgatct caaggacgcg accctcatgc atcagaagtt
     2641 gcaaaaggtt ttcagcgatc ggactgtggg gctcatacac ggcaagctga aggcggccga
     2701 aaaagagtcc atcatgggcc agttcaaagc gggacgcctt cacgttctgg tttccacgac
     2761 ggtcattgaa gtgggggtgg atgtgcccaa tgccacgatc atggttgtgg aacacgccga
     2821 gcgatttggt ttgtctcagt tgcatcaact cagggggcgc gtgggacgcg gggcggagcg
     2881 gtcgtattgt tttctggtgg cgcaatacgc gcaatcggaa gagtcgcgtt atcgtttgcg
     2941 gatcatggag cagacgacca acgggttcga gattgccgaa gaggatttga ggattcgtgg
     3001 ccccggcgag tttctgggca cgaggcaatc gggcctgccg gactttcggt tggccgaaat
     3061 cgtgcgcgat cagcatttgc tagtagcggc acagaagcgg gccgcgctga ttttgcacga
     3121 ggaccctaat ctcaatttgc ccaaaaacgg actcatcaaa aacatcatgc tggagcggtg
     3181 ggggaacagg ctggagttgg ggacggtgta gaataaagag gggagaaagg tacgaccata
     3241 aatttagtca ttgaactaaa tttacttata tggctaaatg aatgtaaata tctctgaaag
     3301 aggttcttat gtcacattca atttccaacc cctataaacc tggcgcgggt catatgccac
     3361 cagttttggc tggaagagag aacgaaataa cagaattccg gatgatactg aaacaatcaa
     3421 ctattctcga aaatctggtt ctcacaggat tgcgcggggt tggtaaaacg gttctccttg
     3481 aaactttcaa acccctggcc attcatgaac gatggctctg ggttggcact gatctttccg
     3541 aatcggctgg ggtcagcgag gaaacgatgg tcattcgctt gctcaccgac ttggctgttg
     3601 tcacatcaca gtttgtgatt cacaaagaag aaaaacacgc tctcgggttt ttgtccaaaa
     3661 aacatttttt ggaggaaaca ctgaattaca cggcactcat gtctctttac aatcgttttc
     3721 cgggtctggt ctctgataga ttaaaagcca tcatggaagt tgtttggcgt gttctttcct
     3781 cgcatcgtat tcaaggcgtt atttttgcat atgatgaagc gcaaaccttg gcagatcaca
     3841 caaaaaaaga tcaatatcct ctctccatgc tactcgatgt tttccaatca gttcagcgta
     3901 aaaatttgcc ttttatgttg ttgctgacag gtttgcccac actttattcc aagttggtgg
     3961 atgccagaac cttttctgaa agaatgtttc gtattattgt tttggacaag ctcacaaaag
     4021 agcaatgcca tgaagccatt gtcaagcccc tgcaagggtc aactgagcgt ttttctgcag
     4081 aatccgtcaa ctcgatcatt gaagaatctg acggttatcc ctactttatt cagtttattt
     4141 gcaaagaagc ctttgacgag ctgatggtaa aaaaagcagt gggtgaaaaa cggaccttcg
     4201 ttgccataaa cgagattacg cgcaagctcg atagggattt ttttgcagga cgatggacga
     4261 aggttacaga acgccagcga gacttactgg ttctaatcgc ccaattgaac aattgcgaca
     4321 aagaattcac agttcaagaa atcgtgaaca aatcaactca aaattctaaa agcccattca
     4381 gtagcagtca tgcgaaccaa ctgttgtcgg cgctcactca atctggactt gtttataaga
     4441 atcgtcatgg caggtactcg ttcgcggtac cgttgctggg aagattcatt cgtcgactgg
     4501 gagaaaacga agacaatgtt gtctgacatt tcatgaattt tagctgattc gtattcccat
     4561 tgtcgaaaaa ccgccacatc aaaaacatca tgctggagcg gtgggggaac aggctggagt
     4621 tggggacggt gtagataacc tgcacgggtg tcgggtcttt ttgtgaaact tgattccctg
     4681 gccaattcga ttatagcaat cccatgaaaa caaattcctt ctcaaagtcc ctttcggtgg
     4741 ccattggcct gttcctggtc ttttttgtct tttccatcgc tcacgcgcaa aatacggtct
     4801 cgacacagct ggaagacaga gcttcccctg tgtccacaaa cactccaaac gtcgacacaa
     4861 ccattgatgc cttcgaggaa attgaaaaat ttcgtgaaag cgcggccttc acggcacaac
     4921 agattgatca attcagtcgc tggaacaacc tcaaagaaaa tcttgattat ctgaccagaa
     4981 attacctgaa gcatttttcc acgtaccaga aaaaaaagaa actatccggt tggagcctca
     5041 atcaggcgag ccaactcaaa ctcaaagcgg aacgattttt tggcaaggcc gctcaataca
     5101 aggaaacgca tcttgagtat ttaaaggata ccgaaacgct catcgccaat cttaacgcac
     5161 aaaagcgatc gttcaaaaaa atgcatcagg atttcagaac ggacgcctca ctcaggacag
     5221 aagccgatct cctgcaaaaa gacattgcca aaatcaacca ggacatcacc acgatcaagc
     5281 aacatcgatc agcatacact gtgttctacg atgtgcacaa tgatgtttat ggttccatcg
     5341 atgagtttga gcgttcgctc gatcgtgaca ttgattattt caaatcctcc ttgtttgata
     5401 aaaatgagcc ccctctctat gacgcagatt tttttgccca gttcactccc catctttttt
     5461 cggagacgat taccatctgg tcggaatcta tttcgttcaa caagaacgct ctatggaaaa
     5521 agaaggcaga gttcattcat ctggcggcgt tgttggccgt gttgtggttg gtgatcagca
     5581 ggctgggaaa acgcgtgaac agcaatctgc tcaaacaccc tctgctcacg tctgttatcc
     5641 tcaccacact gacgggaggg ttatacctgc gtaacgcgaa caatgtcttg atcgtgtttt
     5701 tctgggttgt tttgttcaca ttgctcattt ttctggtgcg caaactgttc gagaaaaaag
     5761 gcacacgcgg tgaagtgatc accctgctgg tcatttatgg ccttatccag atactggaca
     5821 cggtgggttt ggcagtgtgc ttgtaccgcc tcttgctggt agggctaggc ctttacattg
     5881 cgacctactg ttttttaaga tctcgccggc cgaacacaga aaatcttaaa cacgccatcc
     5941 tcctcaccat gattgccttt tttgtgggcg ccagcattat ggaggtcact ggttaccatt
     6001 tgctcaccgt attgctcatt cacggcacca tcaaatccgc ttttttgatt ttcgccatca
     6061 agtatttgag aaagtatttc attgagattc tctccgcgtt gtttctgttg cctggcctgt
     6121 cccgttttca ggtgatcaaa aacaatcacc atttcatcga acgaaaaatc acacgtctgt
     6181 ttcatttgtt cattggtttc gtgctccttt ccgggctgtt ggccatttgg ggggtcttcg
     6241 acacgttcac cttatcctta agcggtgttt ggagttgggg catgagtttg cgcgagcacc
     6301 gcatcacgat tggcatgatc atcactgcca gcgtgattgt ttacctcatc aaggcggtca
     6361 ctcatttttc ccgggcgctt ttggaacaag aatggtatcc ccgtaaaaat gtgagtgagg
     6421 gcacgggcaa ggccgtgaac agcatcatga cctacgggat ctgggttgtg ggacttggtc
     6481 tgattctttc tgttctgggg tttggcctgg aaaagattac aatcatcgcg ggtgctttgt
     6541 ctgtgggcat tggctttggg ttgcaaaaca ttgtgaacaa cttcatttcg ggactcgtgc
     6601 tgttgtttga gaggcccatc aaggtcggcg acattcttgt gatcgatgac gaatggggtg
     6661 tggtcgaaaa agtgggtttg agatcaacca cgatccggaa ttattcacaa gctcatgtga
     6721 tcattcccaa cgcggatttt gtttcccaaa aagtgatcaa cctcacgcac accgatgcga
     6781 aataccgcat taaagttccc atcagcgtgg cctatggatc tgacctgacc aaagtcaagc
     6841 agctgctcga agccttgacc agggcccatc ccaaaatcgc caaacaacca gaaccgctgg
     6901 tcgtttgccg catgctggga gaaagtgctg tgcagtttga tttgttggcc tggctggata
     6961 acgtctccga tagttacatc gtgacggggg atttgttgac agaggccgac agaacattgc
     7021 gtgcccatgg cattggtcta ccgttccccc agcgtgaact tcacataaaa tcagtggatg
     7081 aaaaagtgac gccaggactg ccatcacgtt gtggggacga ccgctttcgt taacttgcat
     7141 ccgcggcgga ggagcgaagg gtgaggatgt tgatcaggca gatgaacgtc accacaaatt
     7201 cagctgaggt gattcccaaa acagaggttg tggccacgag gagggataaa atgaccgatg
     7261 tggtcagccg tgtgcggttg cgcgaaaata gctgttgggt ttgctgcaat aaccacgaaa
     7321 gtaaacggat gcgcgagccc agaatcagta catccgctgt ttgatcgatg atttgtgttc
     7381 ctgtgtcgac cgatacgctc acatgcgctt tctcgaaggg cagggagtaa tcctgagacc
     7441 ccacaaaacc cacaattttc ccatcggcaa tgtctttttc gatcttggcc gctttttctt
     7501 cgggggtgca tcgtgcgtac actttcgtga gtttgagctc cgttatcaaa tgagagacgt
     7561 catcctcagc gtcaccggtg atgatggcag gttcgatctg gagttcttga atctccttga
     7621 gggtgctgtg aatatctgtc ctgaggacat cggcgaagct catgacccct ctcacaaagc
     7681 gatcataccc accgagcaaa acagtttcgc ccaaggcctc gagatcgtcg atcttgtttt
     7741 tcatgtcgcg cgaaacctgc atctgcattc ttttgagaaa acgcaagtta cccagagcgg
     7801 cgaagaaacg gtgatcacct ttgctgggat tgatgtaacc gcagacaccc aaaccgggat
     7861 actctttcaa ttcttcaacg gggtgtcttg tgatttcgtg gtaccagggg tgtgtttcga
     7921 gggcctcggc caaaggatga tgggacgcgg cggccagcga aaaaaacgtg cttaacaact
     7981 gcccctgatt gctgccttgt tcgaggtagt gctgagcgta aacaaaggtt ccctgagtga
     8041 gtgtgccggt cttgttgagg tagagcagat cgaggtcggc cagctttggc aaggaggata
     8101 attttttgac cagaatgccc ttattgaaaa ggtttttcct aataacgtgg gtgctgatca
     8161 tgatggtgcc aggatacgag atcaaacaga acgaaagaaa aaaggccaag ccataaccca
     8221 gggacacatc ccaggagagt tgatgaaaaa tgacctgaaa aaatgataag gccatgcctc
     8281 cgaagagccc caaaaaaata aaaaaaagag aaaataaggt tgccttacga aaagggggga
     8341 aatcatcttc cg
//
LOCUS       DATDUN010000780         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_81318, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000780 DATDUN010000000
VERSION     DATDUN010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81318"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..2086
                     /locus_tag="VJC18_10890"
     CDS             422..2086
                     /locus_tag="VJC18_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45929.1"
                     /translation="MINYKHIIIILLVLLSTACAMSGSTGTNAIDTETPASADPISDT
                     ESDLYESDDSAMSIPVTIAKMESVNVEKTTVTQETSSGLQTKTGFAIKTATAPNTYRI
                     VGTADAVNTEQANALLLVNGDLITSHEVADDGSFDATISAVEGDPVMMIPINDADQGA
                     PPVFAQKENSVTTYSFTNSDKINILQTPIMDRGYYYFSQQNDDDTFDLFRRHIDGSYV
                     QVIAEDLVSAPRVVAVSENHDVVMATQNGRVHYLKHSDDVADLDIYEGMAGFLVAWDD
                     PTMIMDVGEPLNLSEGSITPVSVQLFFSDAENGVFLATKKYISADSTEPQEYLLRFYD
                     LDTNEETGLIESINYSNPLFSQGAGEILYVFVPPANAGKAALFSIMMSNGTTAWNNRQ
                     LIYEHEVGPTIMHMASSGKQMIMFVDLLDNTHRFYQLDAITGEALLFAQEDLDLERIM
                     TPYLVVSVESEVEDSEFITCSINTSETGLDWLVYSRVRSGEMDDLYVIDSGTYGQSCS
                     GRYTIDDDGHMVFYRAYNQDDGSEGMPQVSLFDVYSLDPELLPLAD"
BASE COUNT          586 a          595 c          516 g          511 t
ORIGIN      
        1 tacggatacc ggcagtacgg ccaattcagg tggcggttgc agcctgaaca cgcaggcgtt
       61 accaacaaat tacgtgggca tgataggtat gatttggtta ttcaccatca ctttcattgc
      121 cctgcgcaac aggcgacaca cgccatcgaa cacttgagtt gcccaataga tcatctcgaa
      181 gagaggaata aaatcacaaa aggctgccta acggcagcct tttttcgtat ccagccacat
      241 atttgtgccc aagatgatca acaactcttg acagacgcca acaaccctta acaactgaaa
      301 ccaatttttt tatctcaaat gcgacaaatt atgttaaaat aatcactggt gttctctttg
      361 atacctgcaa ctgtggaggg gtattggcca attcacttgt gttgagtcaa tatcgtacat
      421 catgatcaat tacaaacaca tcattattat tcttctggtc ttgttatcaa ctgcctgcgc
      481 catgagcggc agtaccggca ccaacgccat agacactgaa accccggcct cggcagatcc
      541 catttcggat accgagtccg atctctacga aagtgatgac agtgccatga gcatccccgt
      601 cacaatcgcg aagatggaat cggtcaacgt cgaaaaaacc acagtcacgc aggaaacatc
      661 ctcaggtttg caaacaaaaa caggtttcgc tataaaaacc gcgaccgcac ccaataccta
      721 ccgcatcgta gggacagccg atgccgtcaa tacggaacag gccaatgccc tcctgctggt
      781 caacggtgat ctgatcactt cgcacgaggt cgctgatgat ggctcgttcg atgccacaat
      841 cagcgctgtc gagggagatc ccgtaatgat gatccccatc aacgatgccg atcagggcgc
      901 gcctcccgtc tttgcccaaa aggaaaactc cgtcaccaca tacagcttta ccaattccga
      961 taaaatcaac atcctgcaaa cccccatcat ggaccggggc tactattatt tcagccagca
     1021 gaacgatgat gacacgtttg atctattccg caggcacatt gacggatcat acgttcaagt
     1081 catcgccgaa gaccttgttt ctgcgcctcg tgttgtggcg gtctcggaaa atcacgatgt
     1141 ggtgatggcc acacaaaatg gtcgagtaca ttacctgaaa cactcggatg atgtggcaga
     1201 tctggatatc tatgaaggca tggccggttt ccttgtcgcc tgggatgacc ccacaatgat
     1261 catggatgtg ggagaaccgc ttaacctaag cgaaggaagc atcacccccg tcagtgtgca
     1321 actgtttttt tccgacgcgg aaaatggcgt gttcctggcc acaaaaaaat acatcagcgc
     1381 ggatagcacg gagccgcagg aatacctgtt gcgtttttac gatctggaca caaacgagga
     1441 aacgggtttg atcgaaagca ttaactacag taatcccctg ttctcacagg gggctggcga
     1501 aatcctttat gtgtttgttc cgccggcaaa cgccggcaag gccgcactct tcagcatcat
     1561 gatgtccaac ggcaccaccg cctggaacaa ccgacagtta atttacgaac acgaggtcgg
     1621 ccccaccatc atgcatatgg cctcttcggg aaaacagatg atcatgtttg tcgacttgct
     1681 ggataataca cacaggttct accaactcga tgccattaca ggagaggcct tgctctttgc
     1741 gcaggaagac ctggacctcg aaagaatcat gaccccttac cttgtggtct ccgtcgaaag
     1801 cgaggtggaa gactccgagt tcattacctg ctcgatcaac acatcagaga caggattgga
     1861 ctggctcgtc tacagccgcg tccgctcagg tgaaatggat gatctttacg taattgacag
     1921 cggcacctat gggcaatcat gttcgggcag gtacacaatc gatgatgatg ggcacatggt
     1981 attctaccgc gcgtacaacc aagacgacgg tagcgaaggg atgccacagg tctctctttt
     2041 tgatgtctac agcctcgacc ctgagctatt gccgctggct gactaaaagg gacatctaaa
     2101 aaccacccgt ctgcggcgtt gcccactgca cccgcaatac tcacgtactt ccgtgtacgc
     2161 tccgtttgcg ggtttgtggg cgccttgcat ctgggtactt tttagagg
//
LOCUS       DATDUN010000781         1307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_407511, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000781 DATDUN010000000
VERSION     DATDUN010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407511"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VJC18_10895"
     CDS             complement(<1..182)
                     /locus_tag="VJC18_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HLD45930.1"
                     /translation="MIGFLSGNLHRKDEHRAVIDVNGVGYEVFLAKDTLYQLGETGSS
                     VRFEIYTHVNENAFHL"
     gene            complement(310..>1307)
                     /locus_tag="VJC18_10900"
     CDS             complement(310..>1307)
                     /locus_tag="VJC18_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HLD45931.1"
                     /translation="LEKRKLLPVIGVSMFILWSSFIVLVILLMALDLGVLNRKAHIIG
                     AKEALGWTAFWISLALLFNIVVFFIYEHHWFGMGLTSGEALSGKQAVLQFLTGYIIEE
                     SLSMDNIFVIAMIFAYFKVPNKYQHRVLFWGILGAIVFRMMMILAGSALIHRFVWMIY
                     VFGALLVITAFKMFFSNDKPKELEHNYTVIIAKKFLPLTHEFHENRFVIKLEQKTVFT
                     PLFLCLMVIETTDIFFAIDSIPAIFAITRDPFIVFTSNIFAILGLRSLYFALAAMMAK
                     FHYIKVSLVFILGFVGVKMLASHYVHIPTWASLCVICLALIGGVLASLYIPGKKH"
BASE COUNT          400 a          279 c          319 g          309 t
ORIGIN      
        1 tacaagtgaa aggcattttc gttgacgtgc gtataaattt cgaatcggac agagctaccg
       61 gtttcgccta gctggtacag ggtgtctttg gccaaaaaaa cctcatagcc aacgccgttc
      121 acatcgatca cagcgcggtg ctcatctttt ctgtgcaggt tgcccgatag aaaaccgatc
      181 atatgacgat gtgttcacgt gttcacgtgt tcacgaaaaa acgtttatgg ccaacggtct
      241 acagctgtca gctctcattt gagctttcag cagatttacc tctaggataa gcagaacatt
      301 cgtattcaat caatgttttt taccgggaat gtaaagggag gccagcacac cgccaatcag
      361 cgccaaacag atcacacaca atgacgccca agtggggatg tgcacatagt gtgaggcgag
      421 cattttgacc ccgacaaaac ccaaaataaa taccagactg accttgatgt aatgaaattt
      481 ggccatcatg gcagccaggg caaaataaag tgatctgaga cccaaaatgg cgaatatgtt
      541 ggaggtgaac acaataaaag gatcacgggt gatagcgaaa atggcaggga tggagtcgat
      601 ggcgaaaaaa atgtcggtgg tttcgatcac catgaggcac agaaagagag gggtaaagac
      661 cgttttttgc tcgagcttga tgacaaagcg gttttcatga aactcatgtg tgagggggag
      721 gaattttttg gctatgatga cagtgtagtt gtgttccagt tcttttggtt tgtcgttcga
      781 aaagaacatt ttgaaggccg tgatcacgag tagtgccccg aagacgtaga tcatccacac
      841 gaagcgatga atcagggctg aacctgccag aatcatcatc attcgaaaaa cgatggctcc
      901 cagaatgccc caaaataaaa cgcggtgttg gtatttgttt ggaactttaa aataggcaaa
      961 gatcatggca atcacgaaga tgttgtccat gctcagcgat tcttcgatga tgtagccggt
     1021 taaaaactgt aaaacagcct gttttcccga aagggcttca ccagaggtca gtcccatgcc
     1081 aaaccagtgg tgttcataaa tgaaaaacac cacgatgttg aagagcaggg ccagagagat
     1141 ccaaaacgcg gtccaaccca aggcttcttt ggccccaatg atgtgcgcct tcctattgag
     1201 aacacccaaa tccagggcca tgaggaggat gaccaatacg ataaatgatg accagagaat
     1261 aaacatagag acacctatta ccggtaataa cttgcgtttt tcaagat
//
LOCUS       DATDUN010000782         1133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_117543, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000782 DATDUN010000000
VERSION     DATDUN010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117543"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1133)
                     /locus_tag="VJC18_10905"
     CDS             complement(<1..>1133)
                     /locus_tag="VJC18_10905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HLD45932.1"
                     /translation="LLTLFNALGYLSLHQTLASEFCAQLLFAIVGMIFLFASNHLLIN
                     FIGLEIMSLSLCMQVGSHKHFYKSREAAIKYFILGSVASAVLLFGIALLYGAFGTIEL
                     NLIAIKPTAPELAYLKKIAQTMLLFGLLFKIGIVPLHFWVPDVYEGAPAPVTGFMATG
                     VKIAAFAFALNVFDHLDLFCQPQVTQLLTGLAMASLVLGNLLALVQDSLKRMLAYSSI
                     AHAGFILLGLVAGVTESGWSRQDSFVVLFYLAGYLIMTLGAFAIVSLFVSKRAEADQL
                     THLRGLGQQHPILAGTFTLFMLSLIGLPGTVGFIGKYNVIALAVRNGHLALAIIAVLA
                     TMLSVYYYLKPTVYMYLSKDTPKLSPVNEIPLTTCIAITLCAF"
BASE COUNT          324 a          262 c          321 g          226 t
ORIGIN      
        1 aaaaggcaca cagagtgatg gcaatacagg tggtgagtgg gatttcgttg acgggtgaca
       61 gctttggtgt gtcctttgag agatacatgt acaccgtggg cttaagataa taatacaccg
      121 aaagcatggt cgcgagcacg gcgatgatgg ccagggcgag gtggccattg cggacggcca
      181 atgcgatgac gttgtatttg ccgatgaatc ccactgttcc cggcagaccg atgagtgaga
      241 gcatgaagag ggtaaacgtg cccgccagga tagggtgttg ttgtcccaaa cccctcaagt
      301 gggtcagctg atctgcctcg gcccgttttg agacaaagag agatacgatg gcgaaggcgc
      361 cgagtgtcat gatcaaataa cctgccagat aaaacaacac cacgaaactg tcttgacgtg
      421 accaacctga ttcggtgacc ccggctacca accccaataa aataaatccg gcatgggcaa
      481 tggaagagta ggccagcatg cgtttgagac tgtcctgcac caaggccaga aggtttccca
      541 atacgaggga ggccatggcc agacccgtga gcagttgcgt gacctgtggc tggcagaaga
      601 ggtccagatg atcaaacaca ttcagggcaa aggcaaaggc ggcgattttc acgccagtcg
      661 ccataaaacc tgttacaggt gccggcgcac cttcgtagac atcgggtacc caaaaatgca
      721 gtggcacgat ccctattttg aacaggagtc caaacaacaa catggtttgg gcaatttttt
      781 tcaaatacgc gagttctggt gcggtgggtt tgatcgcgat caggttaagc tcaatggtcc
      841 cgaaggcccc ataaaggagg gcgatgccga acaagagaac agcagaggcc acacttccca
      901 gtatgaagta tttgatggcg gcctcacggc ttttgtaaaa atgtttatga gagccgacct
      961 gcatgcagag cgacagggac atgatctcca gcccaatgaa attgatcagc aagtgattcg
     1021 atgcgaataa aaaaatcatc ccgacgatgg caaacaacaa ctgggcacaa aattccgagg
     1081 ccaatgtttg gtgcagactt aagtaaccca gggcattaaa gagagtgagg agg
//
LOCUS       DATDUN010000783         3153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_241632, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000783 DATDUN010000000
VERSION     DATDUN010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241632"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="dnaJ"
                     /locus_tag="VJC18_10910"
     CDS             complement(<1..924)
                     /gene="dnaJ"
                     /locus_tag="VJC18_10910"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HLD45933.1"
                     /translation="MSKRDYYDLLGIPKSATEADIKSAYRKKALEYHPDRNPGNHEAE
                     EKFKMVAEAYDVLSDTRKRGIYDQFGHAGLQGGPGGGAYHGFNSADDIFSAFGDVFED
                     FFGFGGGRGSSRTRARRGRDLQIETKIDFIEACFGVTKEVSVAHNMPCDACGGTGAKA
                     GSSPETCSYCRGRGQVQMSQGFFTISTTCPQCQGQGRVVSEKCAQCFGRGTVNKKKKF
                     DVKIPAGIQEGMRVMMQGQGEAGENGGPLGDLYVYVHVKEHEEFDREGDHILSEIQVP
                     FPALALGCELDVATIGGAAKIKIKPGTQSGDV"
     gene            1130..>3153
                     /locus_tag="VJC18_10915"
     CDS             1130..>3153
                     /locus_tag="VJC18_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45934.1"
                     /translation="MTIIEGMGILSPINPWDLARTAGFDLTASMNHMLPPTLAQMDYV
                     TSLTARRGLSEHIVSQIHIARRFAQSTWSLPQDTHRLSRSGITNTRPVMLAERALHTG
                     AAFLIGLGFGNQVIQNHISNSSRHRDVLELRQMIGDNTELRTALEQSRWTVEQQHWLL
                     KQIAEGIAQNQQKRMTATKATHRYSREDREQIIAGFQEEISPAIKQAYAALPAAIQTM
                     RFWSCWDRGQQLTLLVKLAEKGGCQVDKVYRHLLPAVQAMAKTRWNGGQRSRLLLDLA
                     EAGGHFMQATYEQLASAISGMAPTNWDFEQQHALLTTLAKNGGSDGFQHLPVAIHGLA
                     SLSCASQQKHQFLLLLANNGKHFVDEAFKAIPNAIQCLANTGWTEQQKLKLLTQLAEN
                     AGLRVGWEWGALTTTLREWEATGWSSMQKYNLLTKLAAQTNNPFDLHCRALLTALRAL
                     QEADWHIEQQYHFLTCLAEKPTDDFKQLFDCVPSVVTRISTLGWSVRQQYQFLTLLVR
                     DLKRNAGQVLNKLPDVLITLRDKGWNFEQQDQLVTLVAQSGHANLHYLHFAIKGLSRC
                     WMSSAEQFYYLVSVTRRAGQGAGAAYQILDWLFLNLDRHSGPTMADHFLRFLSGLNDY
                     NAWQVLNELNNHREFFTKTLTPSQLTQHLSLYAEIFARWPRLGFNIMEGL"
BASE COUNT          789 a          890 c          746 g          728 t
ORIGIN      
        1 cacatccccg gattgggtcc ctggtttgat tttgattttg gccgccccac cgatcgtggc
       61 aacatccagt tcacaaccca gggccagcgc gggaaaagga acctgaattt cggataaaat
      121 gtgatcgccc tctctgtcga actcctcgtg ttccttgaca tgaacataca cataaagatc
      181 gcccaggggc cctccatttt cacccgcctc accctgcccc tgcatcatga cccgcatgcc
      241 ttcctgaatc cccgcgggga tcttgacatc gaactttttc tttttattca cagtcccgcg
      301 accaaaacac tgggcgcatt tttcgctcac gacgcgcccc tgcccctgac actgcggaca
      361 cgtggtgctg atcgtgaaga aaccctgact catctgcacc tgacctcgcc cacggcaata
      421 cgaacaggtc tccggcgacg atccggcctt ggccccggtc cctccacacg catcacaggg
      481 catgttgtgc gccacactca cctctttggt cacaccaaaa caggcctcga taaaatcgat
      541 tttggtttca atctgcaaat cgcgaccacg ccgcgctcgt gttcggctcg aaccacggcc
      601 accgccaaac ccaaaaaaat cctcaaacac atcgccaaac gcactgaaaa tatcgtcagc
      661 gctgttgaat ccgtggtacg ccccgccacc cggcccacct tgcagaccag cgtgtccaaa
      721 ctgatcgtag atcccccttt tgcgcgtatc ggacaacaca tcgtaggcct ccgccaccat
      781 cttgaatttt tcttcggcct cgtgattgcc cggattgcgg tcggggtgat attcgagcgc
      841 ttttttgcgg taggcagatt taatatctgc ctcggtggct gatttgggaa tgcccagcag
      901 gtcgtagtag tcgcgtttag acataaggtg acggatttta ataatgtttt tgattttgtc
      961 aaggggtcaa aggaccgttc tctcatatag cgagtttgag gcaaaaaggc aaatgggatg
     1021 gaaatgggtg gaacaaagac aggaggtaaa aggtcccttt tcacccacga tattttctgt
     1081 ccacaattgt ggacggattt ttctttgtta ggacaacatt ccaggtgctt tgacgataat
     1141 agagggcatg ggaatcctct cgcccatcaa tccctgggat cttgcgagaa cggcaggttt
     1201 tgatctgact gccagcatga accacatgtt gcctccaaca ctggcccaga tggactatgt
     1261 cacctctctc accgcacgca gaggtttgag cgaacacatt gtttcccaaa ttcacatcgc
     1321 ccgccgattt gcgcaatcta cctggtcact gccacaagac acacacagac tctctcgatc
     1381 cggcattacg aacaccaggc cggtcatgct tgcagaacgt gccctgcata cgggagcggc
     1441 cttcctcatt ggtctgggtt tcggaaatca ggtcatccaa aatcacatct caaactcttc
     1501 tcgtcacagg gatgtcttgg aactcagaca aatgataggt gacaatacag aactgcggac
     1561 ggctctcgaa caatcgaggt ggactgtcga gcaacaacac tggcttctca agcagattgc
     1621 ggaaggcatt gcgcagaacc aacaaaaacg catgacagcc acaaaggcaa cccaccgtta
     1681 ttcaagagag gacagggaac aaatcatcgc aggttttcag gaagaaataa gtcccgccat
     1741 caaacaggcc tacgccgcgc tacccgcggc catccagact atgcgtttct ggtcatgttg
     1801 ggaccgtggg caacaattga ctctactggt taaactggct gaaaagggag ggtgccaggt
     1861 tgacaaagtc tatcgacatc ttctcccagc cgtacaggcc atggcaaaga cacgctggaa
     1921 tggtggtcaa cgaagccgtc tgttgttaga tctcgccgaa gcaggcggtc atttcatgca
     1981 ggcgacctat gaacagcttg ccagcgcgat cagcgggatg gcgccaacaa actgggattt
     2041 tgagcaacaa cacgcactgc tcaccaccct ggcaaaaaat ggcggcagtg acggctttca
     2101 acacctgcct gttgccatcc atgggctggc gtcactgagc tgtgcgtccc agcaaaaaca
     2161 tcagttcttg ttgcttctgg ccaacaatgg gaaacacttc gttgacgaag cttttaaggc
     2221 cattcccaat gccattcaat gtctggccaa taccggttgg acagaacaac aaaaattaaa
     2281 gctcctcacc caacttgcag aaaacgcggg acttcgcgtt ggttgggaat ggggggccct
     2341 aaccacaacg ctacgcgaat gggaagcaac aggatggtca tcaatgcaaa aatacaacct
     2401 cctgacaaag cttgccgcgc agacaaataa cccttttgat cttcattgca gggcgctcct
     2461 gaccgcgctc agggcgctgc aagaggcaga ttggcacatt gagcaacagt atcacttcct
     2521 cacttgtctg gcagaaaaac ctacggatga tttcaaacag ctctttgact gtgttccaag
     2581 tgtggtcaca cgtatctcca cactgggatg gtcagtgcga caacaatatc agtttttgac
     2641 tttgctcgtc agggatttga aaagaaatgc cgggcaggtt ctgaataaac tgcccgatgt
     2701 tcttattacg ctacgtgata aaggatggaa cttcgaacaa caagaccagc tggtgacact
     2761 cgtcgcccaa agtggtcatg ctaatcttca ttaccttcac tttgccatta aggggctaag
     2821 tcgttgctgg atgtcctctg ctgagcaatt ttattacctc gtctccgtaa cacgtcgtgc
     2881 cggccaagga gcgggagcgg cgtaccaaat cctcgactgg ctgtttctga atcttgaccg
     2941 gcacagcggc cccaccatgg ccgaccactt tttgcgtttt ctttcgggat tgaacgacta
     3001 caacgcctgg caggttttga acgaactcaa caaccaccgt gaatttttca caaaaacatt
     3061 gaccccgagc cagttgaccc aacacctttc cctgtatgct gagatttttg cacgctggcc
     3121 cagactgggt ttcaacatca tggaaggcct gat
//
LOCUS       DATDUN010000784         3793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_81620, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000784 DATDUN010000000
VERSION     DATDUN010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81620"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VJC18_10920"
     CDS             complement(<1..179)
                     /locus_tag="VJC18_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLD45935.1"
                     /translation="MQYHEPVFRPPSEASSFILQVTLGCSWNKCAFCEMYTSKEFRPR
                     PFANIARDIEWMAKR"
     gene            complement(458..922)
                     /locus_tag="VJC18_10925"
     CDS             complement(458..922)
                     /locus_tag="VJC18_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45936.1"
                     /translation="MTTYHLGLDSPSFTNQKTADVPEPLTETQSDNFFHIESRFDSGI
                     GWSEYTALFKTNKLRCFAPLIFAKRQRAFGLLEQNWPQTSLSTSPGFDYFPTTLTTGI
                     GLEFPLGRYLALDIVGGYQFPPTYLPDSSSRSGQGPMIGIRIFGRRKSDQNN"
     gene            complement(1217..1906)
                     /locus_tag="VJC18_10930"
     CDS             complement(1217..1906)
                     /locus_tag="VJC18_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45937.1"
                     /translation="MTATQIMGAAFLASLALDLPGKDELAAAIFMLLAPPYALTFSTK
                     AKETNVGHYELKQTVAGHAYRVWNPATESELRIDPSDQVLEIHLNNKKLGKTVNEAKG
                     QSAREVFTGVMANERESLLSEIQKILSENPQIKAIRLTTPLDPIYFSWAQDFMIDEYH
                     LSENAERKPSRLKQLLASLIHLSSTQLHPITTGRYMRFMVQRTLKALINQPYPMRTYM
                     IPAEKISQITP"
     gene            2857..3180
                     /locus_tag="VJC18_10935"
     CDS             2857..3180
                     /locus_tag="VJC18_10935"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLD45938.1"
                     /translation="MSELFIRHADICKTFSNPKRLEVISVLGGGIELTASQLLEKIKI
                     SKANLSQHMSVLVQKGVVLSRREGTSMFYKLSDERITKACDIMREVLISRLKNEAQVA
                     KRYKR"
     gene            3192..>3793
                     /locus_tag="VJC18_10940"
     CDS             3192..>3793
                     /locus_tag="VJC18_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HLD45939.1"
                     /translation="MIYLDYNATTPIDKPVADAMQALLRENYGNPSSTHEYGLAAKKI
                     VENARWQVATLLGAKSSEIVFTSGGSESNNTVIKGVAHSFCERGNHIITSQIEHPAVL
                     NPCNFLEKQGYQITHVPVDCFGIIDPVDIEKAITPKTILVTIMHANNETGSLQPISDI
                     ATICQKQGVLFHTDAAQSVGKIPVGVNELGVDFLTVAGHKL"
BASE COUNT         1028 a          781 c          902 g         1082 t
ORIGIN      
        1 tctcgtttgg ccatccattc aatatcacgc gcgatatttg caaaaggacg gggacgaaat
       61 tctttgctgg tgtacatctc acaaaaggca catttgttcc aagaacagcc gagggtgact
      121 tgtagaataa acgaactagc ctccgagggt ggccgaaaaa cgggttcatg atattgcata
      181 gggaatttat cgactttgat taatcagcct gattcttatt tgcaagtgat taggggcgtc
      241 gtgattaggg gcgtcgtgtc tccgtcatac gaaatatgta gccaggacca tgactaagtt
      301 tgtcgaggtt atgaacagtg gtgatgtgtc cttgttggtt gccaataggg ctgaaggggc
      361 tctcattaag gctctggccg tttgtcctga ggcggatagg catcttgggg cgctatacat
      421 tatcagcttg tggagactta aagacggaga tcttttatta attgttctga tctgatttcc
      481 tgcgtccgaa aatgcgaatg ccgatcatgg ggccttggcc gctacgagaa gatgaatctg
      541 gcagatacgt cggcggaaat tgatagccac ccacaatatc cagagccaga tatctgccaa
      601 gaggaaactc caagccgatt ccggtggtca gtgtagtcgg aaaataatca aatcccggtg
      661 aagtcgataa tgatgtctgt ggccagtttt gctcgagaag accaaacgca cgttggcgtt
      721 tcgcgaaaat cagaggagcg aaacaacgaa gtttgttggt tttaaacagg gccgtgtatt
      781 ccgaccatcc aattccacta tcaaagcggg attcaatatg gaaaaaattg tccgattgcg
      841 tctctgttag tggctctggg acatctgctg ttttttgatt cgtaaacgat gggctatcta
      901 aacccaagtg ataggttgtc ataaattccc cctttgacaa cctcatttca taaatcgtgc
      961 cattgcgaat gatttttttt atctttgttt ttcagcagga tgccaaatgt tgatgttgct
     1021 tgacttaaaa aatgttcaaa aagtcacaca ttcttgttca attcttcaca cactattttc
     1081 caaatagtta gaaagtgttt ctcagatcag aacagcgagt cacgtctccg tcatgctgtt
     1141 tctatttgtt tgtgtatgtt tgtgtatgta tgtatgtttg tttgtgtatg acggagacct
     1201 gttttttgag gtgtctttaa ggtgtaattt gactaatttt ttctgcggga atcatgtaag
     1261 ttcgcatggg gtaaggttga ttgatgagtg ctttcagtgt gcgttgtacc atgaatctca
     1321 tgtaacgacc tgtcgttatg gggtgcagtt gtgtggatga caggtgaatc aaactagcca
     1381 ataactgttt caatcgagaa ggctttcttt cggcgttttc agataaatga tactcatcaa
     1441 tcatgaaatc ctgcgcccag gaaaaataga tcggatcaag cggtgttgtg agtctgatgg
     1501 ctttgatctg aggattctct gatagtattt tctgaatttc cgacaacagg gactctcttt
     1561 cattcgccat aacacccgta aatacctcgc gggcactttg tcccttggct tcgttgactg
     1621 tctttcccaa ttttttatta ttaagatgaa tctctaggac ttggtctgag ggatcaattc
     1681 gcagttcaga ttctgtggca gggttccaga cccggtaggc atgcccggct accgtctgtt
     1741 tgagttcgta gtggccgaca tttgtttctt tggccttggt agaaaaggtg agtgcatagg
     1801 gaggggcgag taacataaaa atggcggcgg ccaattcgtc cttgcctggt aggtcaaggg
     1861 ccagcgaggc cagaaatgcg gcacccataa tttgggtggc tgtcacaagg tgtttgtgaa
     1921 taggaccgga tttgtatcga agtccttgat agattcgtgg gacggaaccc aaaataccat
     1981 tgggccaaac gtattccttg ttaatacccc ccctttttgg ctgttgagcc aacctagggg
     2041 gaatactggt ttgtgaaggg gcaacgcgaa gagtcacatt atttggttgc acaatgcttg
     2101 ccacatgcaa gccactattt gtgtcaagca ctgacgctga cggaggagta agataaggaa
     2161 agacgattcg agaagaaata ttcatccaag ggtattatcg gcaatttcag cctgttgttg
     2221 cgtaaaattt gttacctgaa ttccgcactt gacagtgctc cattcaggcc aaaccgctga
     2281 aatcgctgaa tctctgcgtt gctcaggaat ttttaaatcc tcatgtattt ccatatacat
     2341 tccggtttaa aaatccctga gcgccttgat cttcaacgat ttgatcggtt ttctaaattc
     2401 cgacttgcgg aatttaggtg ttatgacgga gtcccaattt tgctccaaac aatcaaggca
     2461 gttttgctcc cccgtaaatc atcacctttt gttgattccc aatcacgata gcttgccccg
     2521 cctctggcaa gctgcatatt cgtgatcggg aaaccaaagc acaaaaggct gctgattaac
     2581 caaattgggg gagcaaaaac tccccctttt gttttttgga ggacctatga acaccaacga
     2641 aaagatttat gatcatatta acaaatcata tttgataaat tcaaacaggg agtcattccg
     2701 tggaaagcca cgtggtacat ggacaggcgc tcgccaatgt acacgtgaca ttattaaaga
     2761 ctacaaaaca tttgacttgt agttttaacg gcgcttatat agttaatagg tttctaaaca
     2821 atgaggttgc taaactcata acagaaaggt gttaaaatgt ctgagctgtt tattaggcat
     2881 gccgatattt gcaaaacatt ttcaaaccca aaacggttgg aggtcattag cgttcttgga
     2941 ggcggtatcg aattaactgc aagccaactt ttggagaaaa ttaaaatcag caaggcaaac
     3001 ctgtcccaac acatgtctgt tctcgttcaa aaaggagtcg tgctttcgag acgagaaggc
     3061 accagcatgt tttacaagct ttcagatgaa cgcatcacca aggcctgtga catcatgcgt
     3121 gaggtgctga tcagcagatt gaagaacgaa gcccaggtag ccaaacgata caaaagatag
     3181 gctggatgct tatgatttat ctcgattaca acgctacaac acccatcgac aaacctgttg
     3241 ccgatgccat gcaggccctc ttgcgggaga attatggcaa tccatcaagt acgcatgaat
     3301 acggtctggc ggcaaaaaag attgttgaaa atgcccgatg gcaggttgct accttgttgg
     3361 gtgccaaatc ctcggaaatt gtgttcacaa gcggcggtag cgaatcaaat aacacggtca
     3421 tcaaaggggt cgctcattcg ttttgtgaac ggggcaacca catcatcacg tcacaaattg
     3481 aacacccagc ggttttgaac ccctgcaatt ttctggaaaa gcaagggtat cagataaccc
     3541 atgttcctgt tgattgtttt ggtattatcg atcctgtcga cattgaaaaa gcaataacac
     3601 caaaaacaat tttagtaacg attatgcacg caaacaacga aacggggtca ctgcaaccga
     3661 tttcggacat tgcgactatt tgccaaaaac agggtgttct ttttcacacg gatgccgcgc
     3721 aaagtgtggg caaaatacct gttggggtta atgaattggg ggtcgatttt ctgacggttg
     3781 caggccacaa gct
//
LOCUS       DATDUN010000785         1671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_117677, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000785 DATDUN010000000
VERSION     DATDUN010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117677"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VJC18_10945"
     CDS             <1..218
                     /locus_tag="VJC18_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HLD45940.1"
                     /translation="LLRDADSANKSWDEAKIKEAIASENEQTIPLRENVPIMIRYRTA
                     WVDEQGNVNLRPDVYDFDAKLAKMLRY"
     gene            263..1621
                     /gene="thrC"
                     /locus_tag="VJC18_10950"
     CDS             263..1621
                     /gene="thrC"
                     /locus_tag="VJC18_10950"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLD45941.1"
                     /translation="MSSLRYYSTNLKAPKVSFREALLKGQAPDKGLYLPELIPSLSVA
                     ERTVFSGQEYHEVAYTIAHKFLHAELPADVLFALVKDAYDYVIPLEQIYDNKYVMRLD
                     QGPTASFKDFAARLMGRLMQYYLKQENKKLLILTATSGDTGSAIANAFYGLTNIEVIV
                     LFPRTEVTPRQRKQMTTLGRNIRIIALDAKFDDCQALVKQAFSDPALSHLHLSSANSI
                     NIGRLLPQSMYYVYAHAKLADNPDQPVIFSVPSGNFGDMMGGLLAKRMGVPVKKFVIA
                     TNANDEFPRFIQNGEYHKIVPSKNCLSSAMNVGHPSNLARVVALYGGNMDETGQIGKL
                     PDLGKMRSDMHAVSVADVATQRTIKQVYDTYGALLEPHGAVGWAGLNDYLFKHPEQDT
                     ADQLCVSLETAHPAKFPELIQKTLGIDPELPPSLVGIEDKTESYETMENDYGVFRKIL
                     ME"
BASE COUNT          423 a          417 c          420 g          411 t
ORIGIN      
        1 atcttttgcg tgacgccgac agtgcaaaca aaagctggga tgaggcaaaa atcaaggagg
       61 cgatcgcttc cgagaacgaa caaaccatcc ccctcaggga gaatgtgccc atcatgattc
      121 gctatcgaac tgcctgggtg gacgaacaag gcaatgtgaa tttgcgtccg gacgtgtacg
      181 attttgacgc caaactggcc aaaatgctcc ggtattgata tagggctcag tcattgacgt
      241 cttctctgac acaggtgaat ttatgtcttc ccttcgttat tattcaacca atctcaaggc
      301 gcccaaggtg tcattcaggg aggccttgct taaagggcag gcccccgata aaggattgta
      361 tttacctgag ctcatcccgt cattatccgt tgccgaacgg acagtctttt ctggccagga
      421 ataccatgag gtcgcgtaca ccatagcaca caaatttttg cacgctgagt tacctgcaga
      481 tgtgttgttt gctctggtca aggatgccta tgattacgtc attcccctcg aacagatata
      541 cgacaacaaa tacgtgatgc gtctggatca agggcccact gcctcgttca aggatttcgc
      601 ggcccgcctg atggggcgcc tgatgcagta ctatctcaaa caggaaaata aaaaattgct
      661 catcctcacg gcgacgtctg gcgatacggg atctgccatt gccaacgcgt tttatggttt
      721 gaccaacatc gaggtgatcg tattatttcc ccggaccgag gtcacgcccc gacagagaaa
      781 acagatgacc acgctgggtc gcaacatccg gatcatcgcg ctcgatgcca agttcgatga
      841 ctgtcaggcc ctggtaaagc aggccttttc cgatcctgct ctttcccatt tgcatctctc
      901 atccgctaat tccatcaaca ttggtaggct gttgccccag agcatgtatt atgtgtatgc
      961 ccacgcaaaa cttgcagaca atcccgatca gcccgtgatt ttttctgtgc cttccgggaa
     1021 ttttggcgat atgatgggag gcttgctggc caaacgaatg ggggtgccag tgaaaaaatt
     1081 tgtcatcgcc accaacgcga atgatgaatt tccccgcttt atacagaatg gtgagtacca
     1141 caagattgtt ccctccaaaa attgcctatc gtctgccatg aacgtgggac atcccagcaa
     1201 cctcgctcgc gtggtggctc tgtatggtgg gaacatggat gaaacggggc agatcggtaa
     1261 attgcctgat ttgggaaaaa tgcgttctga catgcacgcg gtcagtgtgg ctgatgtggc
     1321 gacacaaaga accatcaaac aggtttacga cacgtacggt gcgttgcttg agcctcacgg
     1381 ggccgttggt tgggcgggcc tgaatgatta tttgttcaag cacccggagc aggacactgc
     1441 cgatcaactt tgtgtgtcac tcgaaacagc gcaccctgcc aaatttcccg agttgatcca
     1501 aaagacgttg ggtatcgatc ctgaactgcc acccagtctc gtggggatcg aggataaaac
     1561 agaatcttac gaaacgatgg aaaatgatta cggagtcttt cgaaaaatac taatggagtg
     1621 acgttatttg tggatcgatg tgtcattcca gcgcaggctg gaatccaaaa t
//
LOCUS       DATDUN010000786         1591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_532700, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000786 DATDUN010000000
VERSION     DATDUN010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532700"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /gene="sucC"
                     /locus_tag="VJC18_10955"
     CDS             <1..362
                     /gene="sucC"
                     /locus_tag="VJC18_10955"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863432.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HLD45942.1"
                     /translation="AMATLDIIKLYGGEPANFLDVGGGATRETVTAAFKIILSDPNVK
                     AILVNIFGGIMKCDVIAAGIVSASKELGLNIPLVVRLHGTNVEMGRRILNESGLSIIS
                     ADTMEEAARQAVTCTKT"
     gene            507..1385
                     /gene="sucD"
                     /locus_tag="VJC18_10960"
     CDS             507..1385
                     /gene="sucD"
                     /locus_tag="VJC18_10960"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HLD45943.1"
                     /translation="MSILVNKSTKVLTQGITGSTGQFHTRACRDYGTQMVAGVTPGKG
                     GESFEGIPLFNTVAQAKEATACNASVIYVPPAFAAEAIMEAIDADLDLVVCITEGIPV
                     QDMIKVKRKMVGSRTRLIGPNCPGIITPGECKIGIMPGHIHKPGHVGVVSRSGTLTYE
                     AVDQVTRLGLGQSTCIGIGGDPVNGTDFIDCLKLFNSDNNTHAIVLIGEIGGTAEEEA
                     ADYIKAHVKKPVVAFIAGATAPPGRRMGHAGAIIAGGKGTAQDKFAALSAAGVALATN
                     PAQIGQVLLETISKKK"
     gene            1452..>1591
                     /locus_tag="VJC18_10965"
     CDS             1452..>1591
                     /locus_tag="VJC18_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HLD45944.1"
                     /translation="MSKQRTLSIIKPDAVRRHLIGKILAHIEGAGLKIRGTRLVWLSQ
                     NQA"
BASE COUNT          417 a          427 c          424 g          323 t
ORIGIN      
        1 tggccatggc caccctggac atcatcaaac tctacggcgg cgaaccggca aactttctgg
       61 atgtgggcgg tggcgccacc cgagagactg tcacagccgc cttcaagatc attttgagcg
      121 atcccaatgt taaagccatt ctggtcaata tttttggcgg gatcatgaag tgcgatgtga
      181 ttgccgcagg gattgtgtca gcgagcaaag aactgggttt gaacatccct ctcgtggtcc
      241 gcctgcacgg aacgaacgtg gaaatgggca gaagaatttt gaatgaatcg gggctctcca
      301 ttatttccgc cgatacgatg gaggaggctg cacggcaggc ggtgacttgc acgaaaacat
      361 gagggacgag ggacgaagga agaagagtga aaggcatatg aatatcgaat aacgaacaag
      421 gaacaaggaa tttcgacatt cgacatccct tgttcgatat tcgatattcg ttggcttttt
      481 cgtttgattc acgaaagatt gctattatga gtatacttgt aaacaaatcc acaaaagttc
      541 tcacccaggg catcaccgga tcaaccggtc aattccacac ccgtgcctgt cgtgactacg
      601 gaacccagat ggttgccggc gtcactcccg gcaagggcgg tgagagcttc gaggggattc
      661 ccctcttcaa caccgtcgcc caggccaagg aggccacagc gtgcaatgcc tctgtgatct
      721 atgtgccacc tgcttttgcc gcagaggcca ttatggaggc catcgacgcc gatctggatc
      781 tggtcgtttg cattaccgag gggatccctg tgcaggacat gatcaaggtc aaaagaaaaa
      841 tggtgggctc acgaacacgc ctcattggac ccaattgccc gggcatcatc acaccgggcg
      901 agtgcaagat cggcatcatg ccgggacata ttcacaaacc gggtcatgtg ggggtggtct
      961 cgcgttcggg cacactcacc tatgaagccg tggatcaggt cacacgcctg ggactgggtc
     1021 agtccacctg tatcggcatt ggcggtgatc ccgtcaacgg cacggatttt attgattgcc
     1081 tcaagctctt caattcggat aataacacgc acgccattgt gctcatcggt gaaattgggg
     1141 gcacagctga ggaagaggcc gcggattaca tcaaggccca cgtcaaaaaa cctgtcgtcg
     1201 ccttcatcgc cggggccaca gcccctccgg ggagacgcat gggacacgca ggtgccatca
     1261 tcgcgggcgg caagggaacc gcccaagaca aatttgccgc cctttcagcg gcgggtgtgg
     1321 ccctggccac gaaccccgca cagattggac aagtcctttt ggaaacgata agcaaaaaaa
     1381 aataaaaaca accactccca atggcccggt tcaacttgta aaattttaca aacccacttt
     1441 caggagaaaa catgtcaaaa caaagaaccc tatccatcat caaacccgac gcggtgagaa
     1501 gacacctcat tgggaaaatt ctcgcccaca tcgaaggcgc cggactcaaa atacgcggca
     1561 cacgcctggt gtggctcagc caaaatcaag c
//
LOCUS       DATDUN010000787         3449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_117836, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000787 DATDUN010000000
VERSION     DATDUN010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117836"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="VJC18_10970"
     CDS             <1..1780
                     /locus_tag="VJC18_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45945.1"
                     /translation="ARLPVIVRNFQSEENGQADDQDTFTIQTHDGQIVESSQGKIVAV
                     VEGMLQSPTFEFVQSSGPDVVIEAPQITNFGDKLKLVSHITAPKYEPNNSRAFIKLTV
                     TDQDITKSATTMLAIMPKEKELNKQDVSNMSGQILGVNGDGDLIKLVKEPGNGEMLSI
                     VWLSPTVKLYVSNTAVVQTITIGDYVVLSDYTFQHNRGAIVAFNMPKLKRTEYYLYDA
                     VANSYPGIKSYVAPNEGDRAGYVLDKGDLDGDGTEEVWALLPMGENGAGIFVFSLETM
                     VAISRFVESPFNEFSKLLTIADINADTKADLNTGTVAYDSNLVTADDIDSQGQYNAAD
                     QIMSFLSSGTEKGVLQMADMSASFEIKPQFPELTPKYKGLIKTATMGGANGLIELTTD
                     LDFNNDAADDIATADSNFLISFYFGSPESEETMASMSIDLSEAVDAILSMAAGDVTGD
                     GVDDLVLGCNDGIIIIIPGSTLYPEEAQLSEDDLIIIFADAGVDVDNMVVADTNGDGI
                     DDITFYDDGKDNTTVIDTYDNFGGNNDNTGNNTTAPVFAGKLSGVAGCSLNPTIVVFS
                     ETYHDYLTVAMLLLVLLCVRLRRGTS"
     gene            2062..2559
                     /locus_tag="VJC18_10975"
     CDS             2062..2559
                     /locus_tag="VJC18_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45946.1"
                     /translation="MKKTITLNHNLGHINTSQLPNPADHINEGKPREKEVASSVPLNI
                     CNEIVDALVEQRNVYPLLVQINHLLHFGLPLSARPDVLKELNKILGALFYQYRTDHSL
                     FKQAHEVMTLVNKQIDRKPQKDSHLAELEWEELQKTVYLLCDWEDIIKSSSARTLQTN
                     IDSHQ"
     gene            3028..>3449
                     /locus_tag="VJC18_10980"
     CDS             3028..>3449
                     /locus_tag="VJC18_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLD45947.1"
                     /translation="MNPQVKKWVNLFNGSLRSSFKLWVKRIGYYGPTISSVLSEENVP
                     QDIIYLAMIESGFNTSAHSSASAVGTWQFISSTGKMYGLQNGFFTDDRRDLIQSTKAA
                     ARHLNDLYKLYGDWYLAFAAYNAGSGKVNRAIKQARSK"
BASE COUNT          964 a          858 c          753 g          874 t
ORIGIN      
        1 cgcccgcctg ccggtcatcg ttagaaattt ccaatcagaa gaaaatgggc aggccgatga
       61 tcaggacacg ttcacgatcc agacacacga tggccagatc gtggaatctt cacaaggaaa
      121 gatcgtggcc gtcgttgaag gcatgctcca gtcccccacg tttgaatttg ttcagtcatc
      181 gggtccggat gttgtcattg aagccccgca aatcacaaat ttcggtgata aactcaaact
      241 ggtttcacac atcactgctc cgaagtatga accaaacaac agccgcgcct tcatcaaact
      301 cacagtcaca gaccaggaca tcaccaagtc tgccaccacg atgctcgcca tcatgcccaa
      361 ggaaaaagaa ctcaacaaac aggacgtgag caatatgtcc gggcaaattc tgggagtcaa
      421 tggcgacggt gatctcatca aattggtcaa ggaaccggga aatggagaaa tgttgagtat
      481 cgtctggttg tcccccaccg taaagcttta tgtttcgaac acagccgtcg tacaaaccat
      541 aaccattggt gattatgtgg tcctgagtga ttacacgttc cagcataatc ggggcgcgat
      601 cgtggccttc aacatgccca aactcaaacg cactgaatac tacctctacg acgccgtggc
      661 aaattcgtat ccgggcatca aatcatatgt ggccccaaac gagggagacc gagccggtta
      721 cgttttggat aagggtgact tggacgggga tggaacagag gaagtgtggg ccttattgcc
      781 catgggcgaa aatggggccg gaatttttgt tttttctctc gaaaccatgg ttgcgatcag
      841 tcgtttcgtc gaatccccct tcaacgagtt ttctaaactc ctgacgatcg ccgacatcaa
      901 cgccgacaca aaggccgatt taaataccgg gacggtcgct tatgacagta atctggtgac
      961 cgcagacgac attgattccc aaggacagta taatgccgct gatcagatca tgtccttctt
     1021 gtccagcggc accgagaagg gtgtgttgca gatggccgac atgtccgcca gcttcgaaat
     1081 caaaccgcag tttcctgagc tgactccaaa atacaaagga ctcatcaaga cggcaacgat
     1141 gggaggggcc aacggattga ttgagctcac caccgatctt gatttcaaca acgatgcggc
     1201 agacgatatc gccaccgcgg attcaaactt cctgatcagc ttttattttg gttcacccga
     1261 gtcggaggaa accatggcct ccatgtcaat cgatttgagt gaggccgtgg acgctatctt
     1321 gagcatggcc gcaggtgatg tcaccggaga cggtgtggat gacctggtgc tcggatgcaa
     1381 tgatggcatc atcatcatca ttcccggaag caccctgtat cccgaagaag cccaactttc
     1441 agaggacgac ctgatcatca tttttgccga tgccggtgtt gatgtcgata acatggttgt
     1501 ggcagacacc aacggcgatg gaatcgacga tattaccttt tacgacgatg gcaaagacaa
     1561 cacaaccgtg atcgatacct atgacaattt tggcggcaac aatgacaaca cgggaaacaa
     1621 caccacggca ccagtttttg ccggcaaact ctcgggggta gcaggttgtt cactgaatcc
     1681 caccatcgtg gtcttcagcg aaacttacca tgactacctc accgttgcga tgttgttatt
     1741 agttttgctc tgcgtgagac tcagacgcgg caccagttga ttattttctt gattgctttc
     1801 agccatagga ctgaccccgc tctgcggggc attgcttgat tgcttgattg tttgattgct
     1861 tgattgtttg attgtcctcc ccttctcact ttgataagca atatatttat ttctcatttt
     1921 gcagttctaa tgtcgtcacc tttatgtgac attatcgaca atttacaata gttacaacta
     1981 gttgtctgaa ccttcgccat caaccatttc tggcatgcat gttgcataag tagactatag
     2041 ataacaaaga cgaggttcaa gatgaaaaaa acaattacac tcaatcataa tctgggacac
     2101 atcaatacca gccaattgcc caatcctgcg gatcacatca atgagggcaa accacgcgaa
     2161 aaggaagttg cctcatctgt tcccctgaac atatgcaacg aaatcgtcga cgccctggtt
     2221 gaacaaagaa atgtgtaccc cttgttggtg caaatcaatc acctcctgca ctttggcctg
     2281 cccttgtcgg ccaggcccga cgtcttaaaa gagctgaaca agattttggg ggccctgttc
     2341 taccagtata gaacagatca ctctcttttt aaacaagccc atgaagtcat gacactcgtc
     2401 aacaaacaaa tcgaccggaa acctcagaag gattctcatc tggcagaact cgaatgggaa
     2461 gagttgcaaa aaacagtcta tctcctatgc gattgggaag atattatcaa aagttcatcg
     2521 gcacgaacgc tccaaacgaa catcgattca catcaataac actttgctgt taaccatgtc
     2581 tctattttag tcttgcaata attttgatat ctattattta tcatcaaaac attgacgcta
     2641 atgtcaagtt ttccacagac agtaatggac ttttgcatca ataattttta taatcctcta
     2701 gggtttttaa aaaatgtatt ttgaccccgt aaaataaatc aacaaaagat attttttagc
     2761 aacccacaat agatccataa gctgtcataa ccgtttatat tttacaacag tcatggggag
     2821 gtctttttgc aaaaaaaaac catatcgaat gggttaggaa tagtgctttt cttgtttgtt
     2881 ttgctattat gcgccaattg ttcacatact tcgggttatt cctaccacaa ggtcaattca
     2941 tcctctaaca ggttatctca gggtgatttg gctcaggcac ccgggaccac ttggagacca
     3001 tcaaaaaaaa acacgatacc tgtcaccatg aacccacagg tcaagaagtg ggtcaacctc
     3061 ttcaatggta gtctgcgttc gagttttaaa ctctgggtga aacgcatagg ttactacgga
     3121 ccaaccatca gctcggtcct gtccgaagaa aacgttcccc aggacattat ctatctggcc
     3181 atgatcgaaa gcggatttaa cacctcggca cactcctcgg ccagtgccgt gggaacttgg
     3241 caattcatct cctccaccgg taagatgtat ggtttgcaga acggcttttt tacagacgat
     3301 cgtcgcgacc ttatccaatc cacaaaagcc gccgccagac acttaaacga tctgtacaaa
     3361 ctctatggcg attggtatct ggcctttgcc gcttacaacg caggctccgg caaggtcaat
     3421 cgggcgatca aacaggccag atccaaaaa
//
LOCUS       DATDUN010000788         5359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_241954, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000788 DATDUN010000000
VERSION     DATDUN010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241954"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..3606
                     /locus_tag="VJC18_10985"
     CDS             40..3606
                     /locus_tag="VJC18_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45948.1"
                     /translation="MGSIQLQTGFCSAGLPLVFNEVPGILGSHPVRELQILDGFVASG
                     SHSDKPAINLVPTNTLWSCAPRTPFHTLAQQGISWADQAAVLMRGAAVMTGPSLRLGQ
                     TLEQRLEQKIRFQLVVDSMKTAFEDLYSRGRLATYNKHGLQFEYVKVRREDAPLLVHM
                     GSMGLRFGPSLFVVDDFLPGLSFEDNEMVMDMVAVHEFGEMAFNDHNRASLLEFAIAQ
                     REGVLGRYLELLKSKYQLKFRDVSINRMMEYLEGATENTDDVVDEPDVASDTDKGARD
                     SIALATQYADSFVWPRELYRRFHTALDDPDSKIEEKKFEEYAKRMTCNEQLKHFLDHA
                     TNQVLQSIRNLPFDVEPLDCYRVAQNAFYRMLVPMESELDEGVLDIRMADAGMLTQTL
                     GEIGEQIAQAISQKTRNSFEHPVRKVFWERSLAEHLKILKAQKRFLEHVHHFAINGLP
                     REAHYTVLAGAWNAWKMTEPHPFGRLEGLLTKLADRRMDIMNALSGMRELTEKLNGVS
                     QEEQAELVQILVARQLIDLRKKFSNLTWGDLGFVAQLICLGAVAGGGVRAFERCCVCL
                     PDWMSIKTDVDSTLQQWGGRGSVEKKIVIKRYSLAQKDEDRQTALEQVSTVYFSAGET
                     GVQELERDLGVGVVSRALAIELLVRKCRKSGPRLIPKLWLIPVFAKKFELTPKDIAGL
                     VKMVLDSELAIPALMSYPSLRDLSAEVMRLYAHYLRTDVDCRAVVEWAWERLPDVKAG
                     ATENLAPLSEGLDHFANDHARSPCASVTAVFSTTDIWMWSLATNRLDLQREEQRFETS
                     QLDPEDAQVIAMARTVCDGISFDNRGRPSHPDVLFGDGTFAALASLWNRPHLHQDLSF
                     LVNAFGKTRQMSVGQDEQILFDDVVRYWLISKYYLKKQGQGCEYLGRVLEMEGRGLDV
                     YAACEDNALAKMGLMVRDKRYELWEDFSHDDKNNFLAGIFSWHELMEYVVQHEFPVDV
                     VGRYLQAEALKDKLACVEKYHEEAGKKWIRYGTIILKHNPDQFTEYAQALHSCYLDAA
                     LPTAVPEFILAVMNSEMPADDAVRFFGLVDERLQNDPDLWLATARHVRRFVREYPHRC
                     YNILYYLFNRSRKSFVKWSINDLVCLCRLYSFFALATPLGNEPHGHLRVLVMDELSSR
                     KSSPKPRQIMEEAILRAAKELGLRTKADRLDFWYSLGWARGT"
     gene            complement(3693..4829)
                     /locus_tag="VJC18_10990"
     CDS             complement(3693..4829)
                     /locus_tag="VJC18_10990"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45949.1"
                     /translation="MERSIKKQIIKDLEKKMVFVSGPRQAGKTTLANSLTSVWPNVTT
                     LNYDSDADRPVFLKETWDKNSDLVVFDEIHKYGNWKNKIKGVFDKYGIPPRMLITGSA
                     RLNILKRTGDSLAGRFFHHRLYPFSYKELTDQGCVSKTTLQDMLTLGTFPEPFLSGSE
                     TDARRWRKDYLTLVLTQDVTQLEDVRDLSSMQLLVDLLRERVGSPVSYKSLAGDLQIS
                     PTTVNRYITILERLYLVFRLTPFHKNIARSLLKEPKIYFFDVGLVTRDGGARFENLVA
                     LHLLKHAHFIEDTQGYQTQLCYLRDREKHEVDFFLIDDDSSQGHLIEAKTSDIKPDGS
                     IRYFGARIPNKKRSLQVVLNSDRTLQYGDLKCVPASTLFSELAI"
     gene            complement(4930..>5359)
                     /locus_tag="VJC18_10995"
     CDS             complement(4930..>5359)
                     /locus_tag="VJC18_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45950.1"
                     /translation="HSEIFGGIFDVIVQAGQNGPSPEQRVLYDDFVRREKKIKHGILE
                     KIMEYYNELRVEYLDDMGLDEHHDDDREDISVYIPKVKSTDDLHKIIKPVCVFVPDQT
                     GKNKDRIGLLFDCSWDDRNGLGVMIKGKVLDDIGPKSICY"
BASE COUNT         1426 a         1092 c         1397 g         1444 t
ORIGIN      
        1 aagaaacttt tgatacgaca atgacgataa gcccgccaca tgggatctat tcaacttcaa
       61 actggttttt gtagtgcggg gttacccctt gtgttcaatg aagtcccggg aattctggga
      121 agtcatcctg tacgagagtt gcagatttta gatggttttg ttgcttccgg aagtcactcc
      181 gataagccag caatcaatct ggtgccgaca aacacactat ggagttgtgc tccgagaaca
      241 cctttccata ccttggctca gcaagggatt tcctgggccg atcaggcggc tgttttgatg
      301 cgcggggccg cagtcatgac gggaccatct ttgagacttg gccagacgct cgaacagaga
      361 ctcgaacaaa aaatacgttt tcaattagtc gtagattcca tgaagactgc cttcgaggat
      421 ctttacagca ggggccggct ggctacatac aataaacacg gtttgcaatt tgagtatgtg
      481 aaagtgcggc gtgaagatgc acctcttttg gttcatatgg gtagcatggg actacgtttt
      541 ggacccagtc tctttgtggt ggatgatttt ctgccaggat tgtcatttga agacaacgaa
      601 atggtgatgg acatggtggc cgttcacgaa tttggagaga tggcatttaa tgatcacaac
      661 agggcaagtc ttctggaatt tgccattgca caaagagagg gcgtgttggg gcgatatctg
      721 gaactcctca agtcaaaata ccagctaaag ttcagggatg tttcgatcaa cagaatgatg
      781 gaatatctgg aaggtgcgac cgagaatacc gatgatgttg ttgatgagcc ggatgtcgca
      841 tcagatactg ataaaggagc acgggattcc atagccttgg caacgcagta tgcagatagt
      901 tttgtttggc ccagggaact ctatcgtcga tttcatacgg cactcgatga tccagattct
      961 aaaatcgaag agaaaaaatt tgaagaatat gcaaaacgta tgacctgcaa tgaacagctc
     1021 aaacattttt tggatcatgc aaccaatcag gttttacaat ccattagaaa cctgcctttt
     1081 gatgtggaac cgcttgactg ttatcgtgta gcacagaatg ctttttaccg catgctggta
     1141 ccgatggaat ctgagctgga tgaaggggtg ctggatatcc gaatggcaga tgcagggatg
     1201 ttgacacaga ccctaggtga gattggtgag cagattgcac aagccatcag tcaaaagaca
     1261 agaaattctt ttgagcatcc tgtgcgaaaa gtgttttggg agagatcgct ggctgagcac
     1321 ctcaaaattc tcaaggcaca aaaacggttt ctggaacatg tgcatcattt tgccattaat
     1381 ggtttgccac gggaagccca ttatacggtg ctggctgggg catggaatgc ctggaaaatg
     1441 actgagccgc atccctttgg caggcttgaa gggcttctga caaagcttgc tgatcgtcgg
     1501 atggacatca tgaacgcgct gtctggcatg agagaactaa cggaaaagct gaatggggtt
     1561 tcacaggagg aacaggcgga gttggtacaa attttggttg cgcgccagct aatagattta
     1621 cgaaaaaaat tttctaactt gacgtgggga gatttggggt ttgtggcgca actcatatgt
     1681 cttggtgcag tggcgggagg gggtgtccgt gcttttgaaa ggtgttgtgt ttgtttgcct
     1741 gactggatgt ctatcaaaac tgacgtagac agtaccttgc aacagtgggg tggacggggc
     1801 agtgttgaaa aaaaaatcgt gatcaaaaga tattcacttg cccagaaaga tgaggacaga
     1861 caaacagcct tggaacaagt gtcgacagtc tatttttctg cgggagaaac aggagtacag
     1921 gaattggagc gggatcttgg tgttggtgtt gtcagccgcg cgttggccat cgaattgctg
     1981 gtcaggaaat gccgaaagtc aggaccgcgt ttgatcccga aactgtggct gattccggtc
     2041 tttgcgaaaa aattcgaatt aacacccaaa gacattgccg gacttgtgaa aatggttctg
     2101 gatagcgagt tagcaatccc agcattgatg tcgtatccct cattgcgaga tttatctgcg
     2161 gaggtgatgc gcttgtatgc ccattatctg cgaactgatg ttgactgtcg cgcggtggtg
     2221 gaatgggctt gggaaagatt acctgatgtt aaagctggtg cgacagagaa tctggcacct
     2281 ttgagtgaag gactagacca ttttgccaat gatcatgccc gttcgccctg cgcctcagtg
     2341 acagccgttt tttcgacaac agatatttgg atgtggagtc tggcaacaaa tcgtcttgat
     2401 ttacaaagag aggaacaaag atttgagaca tcacaactgg accctgagga tgcccaagtg
     2461 atcgctatgg cccgcactgt ctgtgatggg atttcgtttg ataatagggg gcgtccttct
     2521 caccctgatg tgctgtttgg cgatggcaca tttgcggcat tggcaagttt gtggaacaga
     2581 cctcatttgc atcaggatct ctctttcctg gtcaatgcct ttggaaaaac caggcagatg
     2641 tctgtaggtc aagatgagca gatactgttc gatgatgttg ttcggtattg gttgataagc
     2701 aaatattatt tgaagaagca gggacagggt tgcgagtatc tcggcagagt tcttgaaatg
     2761 gaaggaaggg gacttgatgt gtatgcggcc tgcgaagaca acgcgctggc caaaatggga
     2821 ttaatggtta gagacaaacg gtacgaactt tgggaggatt tttctcatga cgataaaaac
     2881 aatttcctgg caggaatatt cagctggcat gaactaatgg aatatgttgt ccaacatgag
     2941 tttccagtgg atgttgtagg cagatacttg caggcggagg ccctaaaaga taaattggca
     3001 tgtgtcgaaa agtatcatga agaggcggga aaaaaatgga ttaggtatgg caccatcatc
     3061 ttaaaacata atccggatca gtttactgaa tacgcacagg cactgcacag ctgttatttg
     3121 gatgcggcct taccaacggc ggtgcctgaa tttattttgg ctgtaatgaa cagcgaaatg
     3181 ccagccgacg atgcggtccg tttttttgga ttggttgatg aacgtttgca aaatgatcca
     3241 gatctttggt tggcaacagc aagacatgtg cggcgtttcg tccgtgaata tccgcatcgt
     3301 tgctataata ttctgtatta cctctttaac agatcaagaa agtcttttgt caaatggtca
     3361 atcaacgact tggtttgtct gtgtcggttg tacagttttt ttgccttggc aacaccatta
     3421 gggaatgaac cacacggtca tctcagggta ttggtgatgg acgaattgtc atcgcgaaag
     3481 tccagcccca aacccagaca gattatggaa gaggcgattt tgagagcggc aaaggaattg
     3541 gggttgagga cgaaggcgga taggctagat ttctggtaca gtttaggatg ggcaagggga
     3601 acatagcaca gaatactcgt ttatcctaac cccacatcca acgccatcat gagggctcag
     3661 acgataatgg cattgcagga ggggtgcgaa gatcaaatgg ctaactctga aaaaagtgtg
     3721 cttgccggta cgcacttcaa atccccatat tgtaaagttc tgtcagagtt gagtactacc
     3781 tggagagaac gttttttgtt gggaatgcgt gcaccaaaat aacggatgct accgtctggt
     3841 ttaatgtcac tcgttttggc ttcaatcaga tgcccttgcg atgaatcatc atcaatcaga
     3901 aaaaaatcaa cttcatgttt ttctcggtct ctgagataac aaagttgcgt ctgatagcct
     3961 tgcgtatctt cgatgaagtg cgcgtgtttg agaaggtgca gagccaccag attttcaaaa
     4021 cgggcaccgc catcacgtgt gaccaatccc acgtcaaaaa aataaatttt gggttccttg
     4081 agaagtgatc tggcaatgtt tttatgaaaa ggtgtgaggc gaaagacaag ataaaggcgc
     4141 tccagaatcg tgatgtatcg attgactgtt gtgggtgaga tttgcaggtc accggccagc
     4201 gatttgtacg acacgggcga gccgacacgc tcacgcaaaa gatcgaccag aagttgcatg
     4261 gaagaaagat ctctcacgtc ttccaattga gtcacatctt gagtgagaac cagtgtgaga
     4321 taatccttgc gccagcggcg tgcatcagtt tctgaaccgg ataaaaaagg ctcgggaaat
     4381 gttcccaagg tcaacatatc ctgaagtgtg gttttcgaaa cgcaaccttg atctgtgagt
     4441 tccttgtatg aaaaagggta aagcctgtga tgaaaaaatc ggccagccaa tgaatcgcca
     4501 gtgcgtttga ggatgttaag tctggcgctt cccgtgatga gcattcgcgg gggaatcccg
     4561 tatttatcaa atacgccttt gatcttgttt ttccagttgc cgtatttgtg aatctcgtca
     4621 aagacaacca gatcgctgtt tttatcccat gtttctttga gaaaaacagg gcgatcggca
     4681 tcggaatcat agttgagggt agtcacattt ggccaaactg atgttaatga gttggccaat
     4741 gtggttttac cggcttgtct aggccctgaa acaaacacca tctttttttc caggtccttg
     4801 atgatttgtt ttttgatcga tctttccata cctgacattt taccagtcaa atggcaattt
     4861 gtcatgacaa atttgcagtc ggctgtaaat ttgtctagcc taaccccaca tcccgcgcca
     4921 gcatgagcat caatagcaga tactttttgg accaatatcg tcgagcacct tgcctttgat
     4981 catgaccccc agaccgttcc gatcgtccca ggaacaatca aacaacaaac caatccggtc
     5041 cttgtttttt cctgtctggt cgggcacaaa aacgcagaca ggtttgatga ttttgtgcag
     5101 gtcatcggtc gacttgacct tggggatgta aaccgaaatg tcctcgcggt cgtcgtcatg
     5161 gtgttcatcc agccccatgt catcgagata ttccacgcga agttcattgt aatactccat
     5221 gattttttcc agaatgccgt gtttgatttt cttttcgcgt ctgacgaaat catcgtacag
     5281 gaccctttgt tccgggctgg gaccgttttg ccctgcctgg acaatcacat cgaaaatccc
     5341 gccaaagatt tcggagtga
//
LOCUS       DATDUN010000789         1425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_81996, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000789 DATDUN010000000
VERSION     DATDUN010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81996"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..789)
                     /locus_tag="VJC18_11000"
     CDS             complement(97..789)
                     /locus_tag="VJC18_11000"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HLD45951.1"
                     /translation="MRDLVFFLFFNIIIFASAVSMGFKTIKKERLRKLLIGTPLSNTG
                     FLKSGLVRICGKVSTKDRTLVAPVSQKACVSYRLVVDNPHNKDDQPIIDDRQCVPFFL
                     DDHSGRMTVDMTGAETCFENDLKLKTSDSKKHKISDELRATLLEKYKLTVSTFVGDIT
                     LHFEETILEPGDTITVLGTAHLGSDGNLIHRGQDGLLLIADGNFETTIKALQKRKNWV
                     YDIKVGKKGFSL"
     gene            complement(811..>1425)
                     /gene="murI"
                     /locus_tag="VJC18_11005"
     CDS             complement(811..>1425)
                     /gene="murI"
                     /locus_tag="VJC18_11005"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HLD45952.1"
                     /translation="DHNVKLIVVACNTASAVALDYLQQRLKTPVVGVIESGAAAALAV
                     TKNKRVGVIGTEGTIKSGVYEKALKTLNPTLTCFSQATGLFVPVIEEGIFGESILQPL
                     FTHYLANFLHKDIDTLILGCTHYPILKKQLGQFLGSGITLVDSAQTTARQVWELLNKN
                     NLLNTQSQSTPDEIFFSDAPERVQNIARKILNNQGLVIKQGGIN"
BASE COUNT          342 a          335 c          354 g          394 t
ORIGIN      
        1 tcagggagcc cctccttatc accgcaaggc gggatcccgt ttgcgggaca aaaaggaggg
       61 gcggtgccct cctgttgctt gttgcgcaat aagatttcaa agagaaaagc cttttttacc
      121 cacttttatg tcgtacaccc agttttttct tttttgcaag gccttgatag tggtttcgaa
      181 gttgccgtcc gcaatcagta acagtccgtc ttgcccccgg tgaatcagat tgccatcaga
      241 acccaagtga gctgtgccca agacagtgat ggtgtctccg ggttccaaaa ttgtctcttc
      301 aaaatgcaat gtgatgtcgc ccacaaaagt gctaaccgtg agtttgtatt tttctaacaa
      361 ggtggcacgc agttcatcac tgatcttgtg tttttttgaa tcactggtct tgagtttgag
      421 gtcgttttca aaacaagttt cagcccctgt catgtccaca gtcattcgtc cggaatggtc
      481 atcgagaaaa aaggggacac attgccggtc atcgatgatg ggctggtcgt ctttgttgtg
      541 tggattgtct acgacgagac ggtaggaaac acacgccttt tgggagacag gcgccacaag
      601 agttcgatcc ttggtgctca ccttgccgca aatacgcacc agtcctgact tgaggaatcc
      661 tgtgttggac aagggggtcc cgatcaggag tttgcgcagt ctttcttttt tgatcgtttt
      721 gaaccccatg ctcacggcag aagcaaagat gatgatattg aaaaaaagaa aaaaaacaag
      781 gtcacgcata atcagccctt ttacggaata tcagttaatg ccgccttgtt tgatgacaag
      841 gccttgattg tttaatatct tgcgggcgat gttctgcaca cgctcagggg catcagaaaa
      901 aaagatttcg tcaggagtgc tttgcgactg ggtgttcagc agattatttt tgttcaacaa
      961 ctcccaaacc tgtctggccg ttgtttgggc ggaatcgacc agtgtgattc cgctccccaa
     1021 aaactggccg agctgttttt tcaggatggg ataatgtgta caccccagaa tcaaggtgtc
     1081 gatgtctttg tgcaaaaaat tagccagata atgggtgaac agaggttgta gaatcgattc
     1141 gccaaaaatc ccttcttcga tgacgggcac aaacaaccct gtggcctggg aaaaacacgt
     1201 gagtgtcgga ttcagtgtct tcagagcctt ttcataaaca cctgatttga tcgtcccttc
     1261 ggtgccgatc actccaaccc tcttgttttt tgttacggca agggccgcgg ccgctccaga
     1321 ttcgatcacg ccgaccacgg gcgttttgag cctctgttgc agataatcca atgccactgc
     1381 gctggctgtg ttgcaggcga ccacgatgag tttgacattg tgatc
//
LOCUS       DATDUN010000790         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_82167, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000790 DATDUN010000000
VERSION     DATDUN010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82167"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VJC18_11010"
     CDS             <1..1017
                     /locus_tag="VJC18_11010"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HLD45953.1"
                     /translation="VGLGGLYVLGTERHEARRIDNQLRGRTARQGDPGQTKFYISLED
                     DLMKRFGSDRISTIMDRLGMEEDDAITHKWITGAIEGAQRKVEGHNFDIRKHVLEYDD
                     VMNQQRTAIYTRRRKILAGQALREELTDMIDILVGSIADQYGPDRKGNVDAAVLDQAM
                     FDQFGFHFSLPSGELTPDLVGQKMYDDTLTFLATKETECGKAILDQAIKFFLLQTLDD
                     LWKDHLLTMDHLREGIGLRGYGHKDPKSEYKREGFTMFENMNFRFAQQAVEKIFKVRI
                     RTEEEVQIKEDVDQNVQETRGLVTRGLSHTIKRVEPKVGRNDLCPCGSGKKYKKCHGQ
                     TEAA"
     gene            complement(1023..1244)
                     /locus_tag="VJC18_11015"
     CDS             complement(1023..1244)
                     /locus_tag="VJC18_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45954.1"
                     /translation="MNSHQHHQHILDILEDVRTISFNMTATGKRLDSEQAKIEQMQDE
                     FQGILSLKTEYDHQSQVLDKTIEKIHRLL"
     gene            1443..1859
                     /locus_tag="VJC18_11020"
     CDS             1443..1859
                     /locus_tag="VJC18_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45955.1"
                     /translation="MQGVQDNRRYPRIEHVSSQQILRLDNFQVPHRNYIITKNMSACG
                     LRFTTNEQLEPSSHFLIYLNDQIIRELSPDSHKWFKLGAYYFSRVVWIHELRPGHYEV
                     GAGFLERESCDAGDIDRFTELVNLDTLESLPEYVTQ"
     gene            complement(1853..>2177)
                     /locus_tag="VJC18_11025"
     CDS             complement(1853..>2177)
                     /locus_tag="VJC18_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45956.1"
                     /translation="ALFTGDITKRAEKNLMIKYREHLKATLLKIAHHGSKTATTSAFV
                     EIIDPDLATIGVGREHQYGFPHKETLARLQAQGTKVLRTDLEGGTGVIVNEGVVKTFA
                     TKRRH"
BASE COUNT          533 a          483 c          575 g          586 t
ORIGIN      
        1 gtggggctgg gcggtttgta tgtgctgggc acggaacggc acgaggccag acgcattgac
       61 aaccaactgc gaggacgaac agcgaggcag ggtgaccccg ggcaaaccaa gttttacatc
      121 tcactcgaag acgatctgat gaaacggttt ggatccgacc ggatttccac catcatggat
      181 cggttgggca tggaagaaga tgatgccatc acacacaaat ggatcaccgg cgcgatcgag
      241 ggcgcgcaac gcaaggtcga ggggcacaac ttcgatattc gaaaacatgt tttggaatat
      301 gacgatgtga tgaatcaaca gagaacggcc atttacacgc gtcggcgtaa aatcctggca
      361 gggcaggctt tgcgtgaaga gctgaccgat atgatcgata ttctcgtggg gagtattgcc
      421 gatcaatacg ggccagatcg taaaggtaac gtcgatgccg ctgtgttgga tcaggccatg
      481 ttcgatcagt ttggatttca tttttcactt ccctcagggg agttgacccc cgatctcgtg
      541 gggcaaaaaa tgtatgatga taccctcacg tttttggcta ccaaagaaac ggagtgcggc
      601 aaggcgatcc tggaccaggc catcaaattt ttcctgcttc aaacgctgga tgacctgtgg
      661 aaagaccact tgctcacgat ggatcacctg cgcgaaggga tcggcctgag aggttatggg
      721 cacaaggacc ccaagagcga atacaaacga gaagggttca cgatgtttga aaacatgaac
      781 tttcgttttg cccagcaggc cgtggaaaag atattcaagg ttcggatccg taccgaagaa
      841 gaggtgcaga tcaaggaaga cgtggatcag aatgtgcagg agacccgggg cttggtgacc
      901 agggggttgt ctcataccat caaacgtgtc gaaccaaagg tcggtcgcaa tgacttgtgc
      961 ccctgcgggt cagggaaaaa atacaaaaaa tgtcatgggc aaaccgaggc ggcgtgatca
     1021 ggctaaagca gacgatgtat tttttctatg gtcttgtcga gtacttgtga ctggtgatcg
     1081 tactctgtct tgagagacag gatgccttgg aattcgtcct gcatttgctc aatctttgct
     1141 tgttctgagt ccaatctctt gccggttgct gtcatgttaa aactgatggt tctgacgtct
     1201 tccaatatgt ctaatatgtg ttgatggtgt tggtgtgagt tcatgtcatt ttacaagcaa
     1261 aggctgtgcc acagtagcgt gttaattgca aacggtatcg tattgaaatt gttgtgatag
     1321 tttttctcag ttttttttgt tgcttcaaaa acagacaata atctgacgaa aggcaggtca
     1381 gattattaat atgaaagaga tttaaggttt actcttttta ctaatcagtc tatgcgtcct
     1441 gtgtgcaagg cgttcaagat aatcggcgtt atccacgtat tgaacatgtc tcctctcagc
     1501 agatcctgcg attggataac tttcaggttc cacaccgcaa ttacatcatc acaaagaaca
     1561 tgtcggcctg tggcctgcga ttcacaacga acgaacaact tgagccttcg tcacattttc
     1621 tgatttatct caatgaccag atcattcgtg agctctcgcc agattcccac aagtggttca
     1681 aattgggtgc ctattatttt tcgcgcgtgg tttggattca cgaattacgt cctggtcatt
     1741 acgaagtggg tgccggtttt ttagagaggg aatcatgcga cgcgggtgat attgatcgtt
     1801 tcaccgagtt ggtcaatctg gacacgctcg agagcctgcc tgaatatgtg actcagtgac
     1861 gccttttggt cgcaaaggtt ttgaccacac cctcgttcac aattaccccc gtgcccccct
     1921 ccagatcggt tctgaggacc ttcgtgcctt gcgcctgcag tcgagcgagt gtttccttat
     1981 ggggaaagcc gtattgatgt tctctgccaa caccgattgt ggccagatcg ggatcaatga
     2041 tttctacgaa cgcagaggtt gttgcggttt tgcttccgtg atgggctatc ttgagcaagg
     2101 ttgccttaag gtgctctctg tattttatca tcaaattttt ttctgcccgt ttcgtgatgt
     2161 ctcccgtaaa gagcgcg
//
LOCUS       DATDUN010000791         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_325772, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000791 DATDUN010000000
VERSION     DATDUN010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325772"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VJC18_11030"
     CDS             complement(<1..608)
                     /locus_tag="VJC18_11030"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HLD45957.1"
                     /translation="MNFTKHVLNISKVVVLLLFVALSMATFAGCHNQQKDAGIDHYTC
                     SMHPQIISDKPGECPICHMTLVPVYKEDHNNHSGSSHQENQDKRDDPATGMIGMTQKS
                     GVNISVDKQDTAGVQTTTVQKMKAVFILRAFGTVAFDPDLAVVQSEYLASLNSAGELK
                     TAAKNRLKLLGMSEAEIIELEKSQKVSTNLYLPDNRAGFWVYA"
     gene            complement(632..1900)
                     /locus_tag="VJC18_11035"
     CDS             complement(632..1900)
                     /locus_tag="VJC18_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLD45958.1"
                     /translation="MKTLSLFILLTCLFPAVVRAEALTLEHVVEVVLATHPQILMARQ
                     EADTARHEKIAQSWFSDPKLGFKFEQVPVGSSGPNSANMKTFSFSQDIVFPATLVVQK
                     ASLNAKYQANQANVTQTSREIIFEIKEAYFSLLAVRELLKINRTIYNQYAQGQVILEV
                     AYKSLADKNPKNAPMTSDGSSLFEGVLMAKIKKAETQARINELVSAEKTLESKLNLMM
                     GHDAPDPVPALTTPQLKRIKLSSKVLEEKLLGQNAALKVLDFMSEKSQKDVALAKLSY
                     VPTIMPEFEYNQRANQTDAYTLGLSLNVPLWIHRHSAQIQSARARQKQAQALWANEKL
                     KTTTELYAIYNELTQQFETLSLYKNNILPTSRSLVDTALVSQSAGGSSSLMTLQKLLG
                     YHQANTTYWEMWRNYQIAYALLEKLVGEDL"
BASE COUNT          565 a          498 c          557 g          670 t
ORIGIN      
        1 gcgtagaccc aaaagcctgc cctgttgtca gggagataaa gattggtcga aactttttga
       61 cttttttcca attcgataat ttctgcctcg ctcatgccaa gcagtttgag cctgtttttt
      121 gccgcagttt tcaactcacc agccgagttg agtgaggcca aatattctga ttggacaaca
      181 gccaggtctg gatcaaaggc cactgtgcca aaggccctca agataaaaac agccttcatt
      241 ttttgaaccg ttgtcgtttg aacccctgcg gtgtcttgct tatcgacaga aatatttacc
      301 cccgacttct gcgtcatccc aatcatcccc gtcgcgggat cgtcccgctt atcttggttt
      361 tcttgatgag acgatcccga atggttgttg tgatcttctt tataaacagg aacgagcgtc
      421 atgtgacaaa taggacactc gcctggcttg tcgctgataa tttgagggtg catggaacag
      481 gtgtagtgat caatgccggc gtccttttgc tggttgtgac agccggcgaa ggttgccata
      541 gacagcgcaa caaaaagcaa caacacaaca accttgctga tattcaaaac gtgttttgtg
      601 aaattcatgc agggttcctt ttttttattg attacaaatc ttcacccacc agtttttcga
      661 gcagggcgta cgcaatctga taattcctcc acatttccca gtaggtcgta tttgcctgat
      721 gatagcccag gagtttttga agagtcatca aggaggaact cccgcctgct gattgagaaa
      781 ccagggctgt gtcaaccagt gaccggctcg ttggcaaaat gttgtttttg taaagtgaca
      841 gggtttcaaa ctgttgagtg agttcattat aaatagcata aagctctgtt gtggttttga
      901 gtttttcatt ggcccataaa gcttgagctt gtttttgccg ggccctggcg gattgtattt
      961 gggcgctgtg acgatgaatc cacagcggaa cgttgagact taatcccaac gtgtaggcat
     1021 cggtctggtt ggctcgttgg ttgtattcaa attctggcat gattgttggc acatacgata
     1081 gcttggccag tgcaacgtcc ttttgcgatt tttctgacat aaaatctaaa accttgagtg
     1141 ccgcattttg accgagcaac ttttcctcaa gaaccttaga agaaagtttg attcgtttga
     1201 gttggggcgt ggtcaatgca gggacagggt ctggggcatc gtgtcccatc attaaattga
     1261 gtttggattc cagggttttt tctgctgata ccaattcgtt gatacgagcc tgagtttcgg
     1321 cttttttaat cttggccatc aaaacacctt cgaacaggct tgatccgtca gaggtcatgg
     1381 gtgcattttt tggattctta tccgccagag atttgtaggc aacctcgaga atgacctgac
     1441 cttgcgcgta ttggttgtaa atagtcctgt ttattttcag taattcacga acggctaaaa
     1501 gagaaaaata ggcttccttg atttcaaaga tgatctctct agatgtctgg gtaacattgg
     1561 cttggttggc ttggtatttg gcattaagcg aggccttttg caccaccagt gtggccggaa
     1621 aaacaatgtc ctgcgaaaaa gaaaaagttt tcatgttggc actgtttggc ccagacgatc
     1681 ccacaggcac ctgctcgaac ttaaaaccaa gcttggggtc cgagaaccag gactgtgcta
     1741 ttttttcgtg acgagctgtg tcggcctcct gacgggccat cagaatttgc gggtgtgtgg
     1801 caaggacgac ctcaacgacg tgttccagcg tgagtgcctc agcccgcaca acagcaggaa
     1861 acagacaggt taagagaata aacaatgata atgttttcaa caacctgtcc aatatgataa
     1921 aaccagcagt tcatcaagaa gatgttaagg gcacctctca gaactctctg ttgtcccgca
     1981 aacgggatcc cgcttcgcgg tgacaaagag ggggcagggg gagttgtaat ttgacaaccc
     2041 gataacccgc atttctcacc gtactaatat tttgatctga gagtgatctt cagatcgagc
     2101 tcgacacgca caatcttacg gggggcacta aatgaacatc agcaaaaaaa tattctttga
     2161 tgtcgtgtgc gtgagtgatt tacaaatcga gctggacacg cacaatttgt caggaggtgc
     2221 tgtgtgaatt tttttgctgt gatactttcg gctttgtccg gatttctttt ttatgtgctg
     2281 ctgtatgtgc
//
LOCUS       DATDUN010000792         1019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_204416, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000792 DATDUN010000000
VERSION     DATDUN010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204416"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VJC18_11040"
     CDS             complement(<1..318)
                     /locus_tag="VJC18_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45959.1"
                     /translation="MKQNQKTIALWVMIFVMAMVMGLVFFQEPQKDSKISFGELLTAI
                     TENGVLEITIQQDEYGGRFKDTYHDGQHFLAVGPMDSEQILKLLKKSDAKISYEKPKA
                     APFW"
     gene            complement(403..>1019)
                     /locus_tag="VJC18_11045"
     CDS             complement(403..>1019)
                     /locus_tag="VJC18_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HLD45960.1"
                     /translation="GTDAGVTAMQMDIKIEGITSEIMVQALSQAKEGRVFILGKMAET
                     LTEHRKEMSQFAPKMEMLKISVDKIKDVIGPGGKHIKGIIEETGAKIDIEDDGSVRIF
                     ANCDEVLKRTIELVREYAADVEEGKIYKGIVKKITDFGAFVEILPRTDGLLHISQISN
                     RRIERVEDVLNQGDTVTVRVIAVDHSGKIKLSMKNIDQDPRDNP"
BASE COUNT          233 a          269 c          219 g          298 t
ORIGIN      
        1 ccagaaaggc gccgcctttg gtttttcgta ggaaatcttg gcgtccgatt ttttcaacag
       61 tttgagaatt tgctctgaat ccatcggccc cacagccaga aaatgctggc catcgtggta
      121 ggtgtctttg aaacgaccac cgtactcgtc ctgctgaatg gtgatttcca aaacaccatt
      181 ctcggtaatg gctgtcagca actcaccaaa tgaaattttg gaatctttct gtggttcctg
      241 aaaaaaaacc aatcccatca ccatggccat cacaaagatc atgacccaaa gagctatggt
      301 tttctgattt tgtttcatgt ttggtctcat aaatcatgag ggcactgtcg tgttttcaca
      361 acagtgccct ctatctaaaa gtgactgtcc aaacaagatg cgtcaggggt tgtcgcgtgg
      421 atcctggtcg atattcttca tcgacagttt gattttcccg ctgtgatcaa cggctatcac
      481 cctgaccgta accgtatcgc cctgattcaa aacatcttca accctttcga tgcggcgatt
      541 tgatatttgc gagatgtgga gcaaaccatc ggtccgcgga agaatttcca caaaggcgcc
      601 aaaatcggtg atctttttga caatgccttt atagattttg ccctcttcaa catcggccgc
      661 gtactccctg accaactcga tggtgcgttt gagcacttcg tcgcaatttg cgaatatgcg
      721 gacagagccg tcatcttcaa tatcgatctt ggcacccgtt tcttcgatga tacccttgat
      781 gtgcttgcca ccgggaccaa tcacatcctt gatcttgtcg acactgattt ttaacatctc
      841 catcttgggt gcgaattgtg acatttcctt gcggtgctct gtcaacgttt ctgccatttt
      901 gcccaaaatg aacacgcggc cctctttggc ctgagacagt gcctgaacca tgatttcgga
      961 cgtgatgcct tcgatcttga tgtccatctg catcgcggtc acgcctgcat cggtccccg
//
LOCUS       DATDUN010000793         1249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_574458, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000793 DATDUN010000000
VERSION     DATDUN010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574458"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VJC18_11050"
     CDS             <1..803
                     /locus_tag="VJC18_11050"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45961.1"
                     /translation="LLINVEPSHPLLFPLLMGLTQEKSNRQYISSLETLEVLQALKAY
                     QKVLVAGREPVIATIRMNDRELLGRIDPKKNNQPIELPLKTLPDRMNLQIVKDSGDVL
                     FYDMKFHYALKEFRDWGLEQGITLTREYFDLKGIAVTPKDFLHGKTYKVVLNFFFADE
                     ADFLVVEEPLAAGVEPVNFALQTSRWGLQRQVTNTQPTLGAYLDHREFRDKKILLFAG
                     FVPRGFYEFTYFINVTNRGQFMTPPAKAMEMYSPEIFGTTGKEEVRVK"
     gene            938..>1249
                     /locus_tag="VJC18_11055"
     CDS             938..>1249
                     /locus_tag="VJC18_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45962.1"
                     /translation="MTQSKLTHKTVWVGATYFIEGLPYMLVRFLAGVFFTDMGVRELY
                     LGFLNFLGIPWNLKFLWAPLLDIFGTKRGWMLKLQATIALLVLVIAVLAGLCRPSGTD
                     SL"
BASE COUNT          293 a          282 c          364 g          310 t
ORIGIN      
        1 ggctactgat taacgttgag ccgtctcatc ccctgttgtt tcccttgctg atggggctca
       61 cacaggaaaa atcgaacagg cagtacatct cgtctctgga aacgctggag gtcttgcagg
      121 cgctcaaggc ctatcagaag gtgctggttg ccgggagaga gccggtgatc gcgaccatca
      181 ggatgaacga tcgcgaactg ctgggccgca ttgaccccaa aaaaaacaat caacccattg
      241 aattgcctct caagaccttg cccgatcgca tgaacctgca gatcgtcaag gattcaggtg
      301 acgttctgtt ttatgacatg aagtttcatt atgccctcaa ggaatttcga gactgggggc
      361 tggagcaggg catcacgttg accagggaat attttgacct caagggcatt gccgtgacgc
      421 caaaagattt tttgcacggc aaaacataca aagtggtcct gaattttttc tttgccgatg
      481 aggccgattt tctggtggtg gaagaaccac tggcggcagg ggtggaacca gtgaattttg
      541 ccttgcagac ctcgcgctgg ggcctgcaac gacaggtgac caacacacaa ccgacgcttg
      601 gcgcttatct ggatcatcgg gaatttcgtg acaagaaaat cctgttgttt gccggtttcg
      661 tgcccagggg tttttacgag tttacctatt tcattaatgt taccaatcgc gggcagttca
      721 tgaccccgcc cgcgaaggcc atggaaatgt attccccgga gatctttggg acgaccggaa
      781 aggaagaggt gagggtgaaa tagaaggctt aagggtgatg ggtgatgggt aatggcgaaa
      841 ggcgtaaggc gtaaggcgta aggcgaaagg gggaagggag aagggtcgcg ggatcgcagg
      901 gtctcggggg gcgaaaggca tgaataacga atatattatg actcaaagca aactgacaca
      961 caaaaccgtt tgggtggggg ctacctattt cattgaaggc ctgccttaca tgctcgttcg
     1021 ttttctggcg ggtgtgtttt ttaccgacat gggggtgcgt gagctctatt tgggattcct
     1081 gaattttttg ggaattccgt ggaacctcaa attcctttgg gctccccttc tggacatctt
     1141 tggaaccaaa cgcggttgga tgctcaaact gcaggccacc attgccctgc tggtcctcgt
     1201 cattgctgtg ctggcgggtt tgtgtcgacc gtcgggaaca gattcttta
//
LOCUS       DATDUN010000794         1069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_574459, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000794 DATDUN010000000
VERSION     DATDUN010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574459"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VJC18_11060"
     CDS             complement(<1..211)
                     /locus_tag="VJC18_11060"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186165.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HLD45963.1"
                     /translation="MSHTVIVSAKRTPVGSFNGSLASIPTTKLGAIAVKAAVDAAKIN
                     PQEVDEVILGNVLPAGEGQSPARQAI"
     gene            653..>1069
                     /locus_tag="VJC18_11065"
     CDS             653..>1069
                     /locus_tag="VJC18_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45964.1"
                     /translation="MAITLAQGKVGPVTDWLSSFVGAAATANAGGVPVINVAPAKIGE
                     QVIVPPHPDQANAWQLRTKKQWLPQHINLLWTPEQLHVARMSKGDATLLPVLKTTKTG
                     RTVWRRGRLGVWPVDANSCFVLSPPERGISGVLKINR"
BASE COUNT          300 a          214 c          284 g          271 t
ORIGIN      
        1 caatggcttg acgtgcgggc gattggccct ctccagcggg gagtacgttg cccaaaatca
       61 cttcgtcaac ttcctgcgga ttgatcttgg cggcgtcaac agcggccttg acggcgatcg
      121 cgcccaattt ggttgtgggg atagatgcca gagaaccatt aaacgaccct acgggggtgc
      181 gtttggccga aacgatcacg gtatgggaca taaaaactcc taatataggt aatgggttat
      241 ggctgaatga ttgcaattgt tgcaattgtc aactgcttca acaatcaatt gtcaatttga
      301 gtagttgata attgagataa ttgatacagt tgaatttata cgttgattga atcactcaca
      361 ggtcatgcta tgccacgcgt atatagaggc tgggacaaag aggcaaatga aaaaccattt
      421 gaatatcgaa tgatgaacag ggaacaagga atgtcgaagt aaagagagtc tttcaatatt
      481 caaatctgca ttgttgtagc cataggtcca aaaatcccat attaatattg aatagttata
      541 acatatgacc gaaaatcaaa aaaatgtgaa aagggcacgc aacttattgg ttatgattac
      601 gataagatgt gggtcgatag aagggtgttt gttattttac caaggttttt taatggctat
      661 tacactcgca caaggtaagg ttggtccagt cacagactgg ttatcctcgt ttgttggggc
      721 cgcggcgacc gcgaacgcgg gcggtgtccc cgtgatcaat gtggcgcccg caaagatagg
      781 tgaacaggtt attgtgcctc cgcatcctga tcaggcaaat gcctggcaat tacggacgaa
      841 aaaacagtgg ttgccgcaac atatcaacct gctctggacc cccgaacaat tgcatgtcgc
      901 ccggatgagc aagggggacg cgactttgct accggtattg aaaacgacga agacagggcg
      961 taccgtttgg cgcagaggcc gtctgggtgt gtggccggtt gatgccaata gttgttttgt
     1021 tttgtcccct ccggaaagag gcataagtgg cgtgttgaaa ataaacaga
//
LOCUS       DATDUN010000795         1444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_408415, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000795 DATDUN010000000
VERSION     DATDUN010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408415"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VJC18_11070"
     CDS             complement(<1..692)
                     /locus_tag="VJC18_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLD45965.1"
                     /translation="MLSIKKLFFIFSILLSFAVHAEDELSGKLTVFHAGSLSVPLKQI
                     VTQFRQLHPNLDVQLEAAGSIDSARKISELKRPCDVLASADYLVIDHILIPEYADWNI
                     KFASNEMTLVYHKNSRRSEELTEANWFEILLDPKISFGRADPNADPCGYRAVLTMQLA
                     ERYYNKPGLAQAILQKETRFMRPKETDLLALLESSTIDYIFLYRSVAEQHGLKYLRLP
                     DQINLKNSQLAS"
     gene            complement(735..941)
                     /locus_tag="VJC18_11075"
     CDS             complement(735..941)
                     /locus_tag="VJC18_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45966.1"
                     /translation="MDDVTLIKTLIEAANKQSENILTLTCQRAHQLAEEYSIELRRIG
                     AICQTEGIKIINCELGCFGKHRHE"
     gene            complement(961..1419)
                     /locus_tag="VJC18_11080"
     CDS             complement(961..1419)
                     /locus_tag="VJC18_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HLD45967.1"
                     /translation="MTPQEIEITFSLATRRELEANAFYARVAKNALNPEVKQVFEQLA
                     NEEMGHFELLERFRTDPTLPLKMTAPPQNYRLAEATPLPPFSDDMLPRDAIALAMKKE
                     QEAVEFYLALSAGTPDAGLKDIFDNLANMELVHKQRLENVFVAEGYPEIF"
BASE COUNT          348 a          324 c          384 g          388 t
ORIGIN      
        1 tggctggcga gttgtgaatt cttgagattg atttgatcgg gcaggcgaag gtatttcaga
       61 ccgtgttgtt cggccacaga gcggtaaaga aaaatataat cgattgtgct cgattccaga
      121 agggccagca gatcagtttc tttggggcgc ataaaacggg tttctttttg caggatagcc
      181 tgggccagac ctggtttgtt gtagtatctt tcggccaact gcatggtaag aacagcgcga
      241 tagccgcagg gatcggcgtt ggggtctgcc cggccgaagg atattttggg atccaaaaga
      301 atttcaaacc agttcgcttc tgtcaattcc tcactgcgac gagagttctt gtgatacacg
      361 agggtcattt cgttgctggc aaacttgatg ttccagtctg cgtattctgg gatcagaata
      421 tgatcgatca caagataatc ggccgatgcc aacacatcac aggggcgctt caattcactg
      481 atcttgcggg cagagtcgat ggagccagcc gcctcaagct gaacatcgag attggggtgc
      541 aactgcctga attgggtcac gatctgtttg agtgggacag aaagactgcc cgcgtggaat
      601 acggtgagtt ttcccgagag ttcatcctct gcgtgtaccg caaaactcaa caggatcgag
      661 aaaataaaaa aaagtttctt gatagaaagc atgaagagac tcctttgtcg tgaaagagaa
      721 agctttgatg ttgttcactc atgccggtgt ttcccaaagc aaccaagttc acaattgatg
      781 attttgatgc cttcggtctg gcagattgcc ccaatgcggc gtaattcaat actgtattcc
      841 tcagccaact gatgcgctct ttgacaggtc aacgtcagaa tattttcact ttgtttgttg
      901 gccgcttcga taagagtttt gataagtgtg acatcgtcca tatgaaaaag atggtattga
      961 ttaaaatatt tccggatacc cctcagcgac gaacacgttt tcaagacgct gtttgtggac
     1021 aagctccatg ttcgccagat tgtcaaagat atccttgagt cccgcgtcgg gtgtccctgc
     1081 ggaaagggcc agatagaatt ccacagcctc ctgctctttt ttcatagcca aggcaatcgc
     1141 atcacgcggg agcatgtcat cggagaacgg ggggagaggg gtggcctcgg ccagcctgta
     1201 attttggggc ggggccgtca ttttcaaggg cagtgtggga tccgtccgaa atcgttccag
     1261 tagctcgaaa tggcccatct cctcattggc aagttgctca aagacctgtt tgacctcagg
     1321 attgagagcg ttttttgcca cccgcgcgta aaacgcgttg gcctctaact cacgcctggt
     1381 tgccaggctg aaggtgattt caatttcctg aggggtcatg gcgtctcctt aattttatga
     1441 attc
//
LOCUS       DATDUN010000796         3823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_574561, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000796 DATDUN010000000
VERSION     DATDUN010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574561"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VJC18_11085"
     CDS             complement(<1..541)
                     /locus_tag="VJC18_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45968.1"
                     /translation="MTQGSIPKAYAEALQCPSCESLRLIPTKEKIIICQDCLTGYRVT
                     NGVPDFRLEHAMSFRNRLNQKKTGLHASLTTVMGENKEQSVEVKYGHCVILGRLVNPS
                     VNLDVTVVLKPVSQLTYSHLDPNNQQLIEKYLSRGQSGTQVRDVGLGAKGKLLGNFTR
                     DPDFLINDPSISRTHAVIYQ"
     gene            complement(544..1062)
                     /locus_tag="VJC18_11090"
     CDS             complement(544..1062)
                     /locus_tag="VJC18_11090"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HLD45969.1"
                     /translation="MTKKVIVLAVLALIFVCAGTQRAWAQEKIALVSLQTALNEVNEG
                     KKIKEGLKVEYETKKKKIDDMKLELETMSQDLEKQQMVLSADALNIKRKELQTKFLDL
                     QNKAATFERELKTKEAQSAQKILTMLRTIVQEIANKSAYTLVIENSTETILFSKNAKD
                     ITTEVIAAYNAK"
     gene            complement(1214..3490)
                     /gene="bamA"
                     /locus_tag="VJC18_11095"
     CDS             complement(1214..3490)
                     /gene="bamA"
                     /locus_tag="VJC18_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HLD45970.1"
                     /translation="MKRIAIFIAIFLLMTTAVQAAEKIVNVDFSGQVSASETNMRSHV
                     TSIEGTTYNEKNVEADIKNLYQTGLFTDVSVQKLGIAGGVKLIFQVVEKKTVGKLTIE
                     GNKKIKKKDLLEAVRITEMSQLDPAKIAETKNAIYKLYEEKGCFLVDVKTSIEPYDPD
                     QNQVELVIKIRENKSVKIRRVQFLGNKIYSDKELRKKIKTKEKGFLSAVTESGKLENE
                     KFDADLHLLRYHYLDNGYINVKVGEPNITLTRDKRSIYITIPVHEGKQFKVSEIDIGG
                     DILTTKEELLGKLKMDVGKIYRKSLEIEDLHTLEKVYGDQAYAFASIYPNLDVNEQKK
                     TVKVTYYIQKGPKVKIGKIIIKGNDVTRDKVIRRELKLVENAYFSQSALELSRTRLYQ
                     LGYFEEVNIATPRGASENLIDLVIEVKEKNTGTISVGAGFSTLENFIFTATVQKENFF
                     GLGWGGGVSANISKLRQDIMLSLSDRYFLDSKWFFGLSFQKFQSQLNRDFDQNRFGGT
                     VTFGREVIDFLHFRFGYMIDDVEVLNFSSQVPQFFQEYASGLTSAVFGTIMYDRRDNR
                     VSTKKGIYTSVRAEYSSDYLGAENNYLQLTNDNRIYFKLPLEFVLKGRGMVGYINSND
                     NRPIPLFNRYFLGGVNTLRGFDINSIGPSISVPQTITGGDLPFVYGGNKMLLVNAELE
                     IPVYAKAGFYLVSFLDGGNAFGEDENFDLTSLRYNYGFGVRWHSPMGPLRFEWGFPIQ
                     KQAGEAGAVFNFTIGQSF"
     gene            complement(3504..>3823)
                     /locus_tag="VJC18_11100"
     CDS             complement(3504..>3823)
                     /locus_tag="VJC18_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLD45971.1"
                     /translation="QETARTMLETVGLGLLTTRHPLELSGGEQQRAAIARALVHRPKY
                     LFADEPTGNLDDQNSNQVFDLLCGLNRDLRATLIVVTHNQAFANRMNRKILLDQGRLM
                     VDE"
BASE COUNT          983 a          944 c          824 g         1072 t
ORIGIN      
        1 cctgataaat aaccgcgtga gtcctcgaaa tggatggatc gttgatcaaa aaatcgggat
       61 cccgtgtaaa attgcccaac aacttgccct ttgcccccaa accaacatct ctcacttgag
      121 tcccgctttg tcctcttgaa agatattttt cgatcagttg ctggttattg gggtcgaggt
      181 gggagtaggt gagttggctc acaggtttga gaacgacggt cacatccaag ttcacgctgg
      241 ggttgaccaa gcgtcctaaa atgacacaat gtccatactt gacttcaacg ctctgttctt
      301 tattctcacc catcactgtc gtcaaagagg cgtgcaaccc ggttttcttc tgattcagac
      361 gattgcggaa agacatggcg tgctcaagac gaaagtccgg cacgccgttg gtgacacggt
      421 aaccagtcag gcaatcctga cagatgatga tcttttcctt cgtggggatc aaacgcaaag
      481 actcacatga cgggcattga agggcttcgg cgtaggcttt gggtattgat ccttgcgtca
      541 taatcatttt gcattatacg cggcaatcac ctctgtagtg atgtctttgg cattcttgga
      601 gaacaaaatt gtctctgtcg aattctcgat caccagtgtg taagcgctct tgttggcgat
      661 ctcttgtaca atcgttctca acatggtgag tattttttga gcactctgtg cctctttggt
      721 cttgagttct ctctcgaacg tggcggcctt gttttgcagg tcgaggaact tagtctgcaa
      781 ttctttgcgt ttgatgttaa gggcatccgc agataacacc atttgttgtt tttcgagatc
      841 ctgagacatg gtctcgagtt cgagcttcat atcgtcgatc tttttctttt tggtttcata
      901 ttcgactttg aggccctctt tgattttttt gccttcgttg acctcgttta aggccgtttg
      961 cagactcaca agagctattt tctcctgcgc ccaagcacgt tgtgttccgg cacaaacaaa
     1021 aatcaaggct aaaacggcta atacgatcac tttctttgtc atataattac tccagtttaa
     1081 attgataaca attacgatac ggtctattta acgatcatct gtctgagaag ttttataaaa
     1141 agatatctca atccagactg acgttatgta cgcgggcgta cagctacaaa aagtcacggt
     1201 caagatcaac taattaaaaa ctctgaccta tcgtgaaatt gaagacggcc cctgcctcac
     1261 ccgcctgttt ttgaatcgga aaaccccatt caaagcgcag tggccccatg ggagaatgcc
     1321 accgaacacc aaatccgtaa ttatagcgaa gactcgtcag atcgaaattc tcgtcctctc
     1381 caaaagcgtt cccaccatcc agaaaactga ccagataaaa accagccttg gcatagacag
     1441 gaatctcgag ttcggcattg accaaaagca tcttattgcc accgtataca aacggcaagt
     1501 caccacccgt gattgtttgt ggcacactga tagacgggcc gatagagttg atgtcgaaac
     1561 cccggagcgt gttcacccca cccagaaaat accggttgaa caacgggatc ggccgattat
     1621 cgttagaatt gatgtaacca accatacccc tgcccttaag gacaaattcc aatggcagtt
     1681 tgaagtagat tctgttgtcg ttggtcaact gcagatagtt gttttcggcg ccaagatagt
     1741 cagatgaata ctccgcccgc actgacgtgt agattccttt tttggtagaa acacgattgt
     1801 cgcgtctgtc gtacatgatg gtaccaaaaa ccgcgctggt gagtccggag gcgtattcct
     1861 gaaaaaactg agggacctgt gaagaaaaat tcagaacttc gacatcgtcg atcatataac
     1921 caaaacggaa atgcagaaaa tcaatcactt ctcgtccgaa ggtgactgtc ccgccgaacc
     1981 ggttctggtc gaagtcacga ttcaattgcg attgaaattt ctgaaaactc agaccaaaga
     2041 accacttgct gtccaaaaaa taacgatcgg acagggacaa catgatgtct tgacgcaatt
     2101 tggaaatatt ggccgaaacg ccgcctcccc agcctaaccc aaaaaagttt tccttttgca
     2161 cagtggccgt aaaaataaag ttttccagag tagaaaaacc tgcccccaca ctgatcgtgc
     2221 ccgtgttttt ttctttgacc tcaatcacca gatcgatcag gttttcggag gccccgcgcg
     2281 gtgtggcaat gttgacctct tcaaaatacc ccaactggta caaacgtgtc cgagacaatt
     2341 ccaatgctga ctgactgaaa taggcgtttt caacaagctt caattctcga cgaatcacct
     2401 tatcgcgcgt cacgtcgttt cccttgatga tgatcttgcc gattttaacc ttggggcctt
     2461 tctggatata ataggtgacc ttgaccgttt ttttctgctc attcacatcc agattgggat
     2521 aaatgctggc aaaggcgtac gcttgatcac cgtaaacttt ctcaagcgtg tgcaagtcct
     2581 cgatctccag ggatttgcga taaatcttgc ccacatccat ctttaatttg cccagcagtt
     2641 cttctttggt ggtcaggata tcgccaccga tatcaatctc ggacaccttg aattgtttgc
     2701 cttcatggac gggaatagtg atatagatgg atcgtttgtc acgcgtcagc gtgatgttgg
     2761 gctcgccaac tttgacgtta atgtaaccgt tatccagata gtgataacgc aggagatgga
     2821 gatcggcatc aaacttttcg ttctcgagct tgcccgattc ggtcacagcg gacaaaaacc
     2881 ctttttcctt ggttttgatt tttttacgaa gttccttgtc tgaataaatc ttgttaccca
     2941 aaaactggac acgtctgatc ttaaccgatt tgttttccct gatcttgatg acaagctcaa
     3001 cctgattttg atcgggatca tagggttcaa tcgatgtttt gacatcgacc agaaaacaac
     3061 ccttttcctc gtacaattta tagatggcat ttttggtttc ggcaatcttc gcaggatcca
     3121 actgagacat ctctgtgatg cgcacagcct ccagcaaatc cttcttcttg atctttttgt
     3181 tcccctcaat ggtgagtttg ccaaccgttt tcttctcgac aacctgaaag atgagcttca
     3241 caccaccggc aataccgagt ttctgcactg aaacatcagt gaacaagcct gtctggtaca
     3301 gatttttgat gtcggcttca acattttttt cgttataggt cgttccctca atggaggtaa
     3361 catgcgaacg catgttcgtc tcggacgcag agacctgacc ggagaaatca acattgacga
     3421 tcttttcggc cgcctgaaca gccgtcgtca tcagcaaaaa aatggcaatg aaaatcgcta
     3481 tccgtttcat caatattccc tcatcactca tcaaccatca gtctcccctg gtccaggagt
     3541 attttacgat tcattctgtt ggcaaaagcc tgattgtgcg tcaccacaat cagggtggcc
     3601 cgtaaatcac ggttaagccc acaaagcaaa tcaaaaacct ggttgctatt ctgatcgtct
     3661 agattcccgg tgggttcatc ggcaaataaa tacttgggcc tgtgcaccag agccctggca
     3721 atggccgccc tctgttgttc gccaccagaa agctccagcg gatgacgcgt ggtcaacaga
     3781 ccaagcccga cggtttccaa catggtacgt gctgtctctt ggg
//
LOCUS       DATDUN010000797         1890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_161114, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000797 DATDUN010000000
VERSION     DATDUN010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161114"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VJC18_11105"
     CDS             complement(<1..649)
                     /locus_tag="VJC18_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45972.1"
                     /translation="MKKTLSVGITILFVGSLWGFLEATLGGLLHLSPLPIAGKIMAPI
                     GFTLLYWAQKNGLKASHLFLAACLAACFKFADVFLFNMPLFSIQIINPAQAIVMQGLC
                     FAGLVKIFPLFDRSHAKLATTSLVFSVHFVLSAAFSLLVFNRVMGAFDIGPLATAIHA
                     IVGTALTAVGLFAIQWTQSRTNVWNVSRLPFAYRFAGSLALVIIGITIRGLIGWSK"
     gene            873..1274
                     /locus_tag="VJC18_11110"
     CDS             873..1274
                     /locus_tag="VJC18_11110"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015591536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HLD45973.1"
                     /translation="MKTVLFLCTGNSCRSQMAEGLLRAMASDEYDVYSAGVAPSTLNP
                     NAVLVMTEMNIDISAQYSKSIDALPKTKFDYVITLCDHAKQVCPVFPGTQQLHWGMPD
                     PFDVVGTEEEKRNAYRACRDALQKRIREFVG"
     gene            complement(1284..>1890)
                     /locus_tag="VJC18_11115"
     CDS             complement(1284..>1890)
                     /locus_tag="VJC18_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45974.1"
                     /translation="FIDLSVGREHACAIDEDNHVLCWGNNSQGQTDVPQAEITTDQSV
                     THVPQEFITISAGEYHNCGLTTKGDVVCWGRNKEAQLHVPDRDFISLSAGGYHMCGIT
                     SDQNALCWGDHSMKQLNAPNTQFMQISSGRNHTCGVTLDKHIACWGSDLNSQAKPKSL
                     PPENFLLVSSGDSHSCAITESGKSVCWGDESMNQSRNEEMK"
BASE COUNT          474 a          435 c          508 g          473 t
ORIGIN      
        1 ttttggacca gccaatgagg cccctgatgg tgatgccgat aatgaccagg gcgagactgc
       61 ctgcaaaacg atacgcgaag ggcagacgac tcacattcca aacatttgtg cgggattgtg
      121 tccattgaat ggcgaacaag ccgactgctg tcaaggcagt gccaacaatg gcgtgtatgg
      181 cagtggctag aggaccgata tcaaaggccc ccatgacgcg attgaataca aggagagaaa
      241 aggcagcaga gagtacaaag tgcacggaaa acaccagcga tgttgtggcc aacttggcat
      301 gggaacgatc gaacagcggg aagatcttga ccagccctgc aaagcacagg ccttgcatga
      361 caatggcctg cgcggggttg atgatctgaa ttgagaagag tggcatgttg aataaaaaga
      421 catcagcgaa tttgaaacag gccgcaaggc aggcggccag aaaaagatga ctggccttga
      481 gcccattttt ttgtgcccag tacagcagtg taaaaccaat gggggccatg attttaccgg
      541 cgatggggag tggagagagg tgcaatagtc cgcccagtgt ggcctccaga aaaccccaga
      601 gagaaccaac aaacaaaatt gtgataccaa cagataatgt ttttttcata gtcatttcct
      661 tgttttgtca tcccagcgga cgctgggatc catgtttacg gtaattctgg attcctgcat
      721 ttcagccgta ggactgatcc cgtttgcggg acgcaggaat gacacttcgt gttagttgag
      781 caagagcggg ttgagattaa caaagactct ttttaaatga aagtacaatt ttctttcttg
      841 tcgtcagttg tctctacagg tattctacgt ccatgaaaac ggtcctattt ctctgcacag
      901 gcaattcatg ccgctcacaa atggccgagg ggcttttgcg tgcgatggct tctgatgaat
      961 atgatgtcta cagcgctggg gtggcgccgt cgaccctgaa tcccaacgcg gttttggtga
     1021 tgacggaaat gaacattgat atttcagcac agtattcaaa gtccatcgat gccttgccca
     1081 agacaaaatt tgattatgtc atcacccttt gtgaccatgc caaacaggtt tgtcccgtgt
     1141 ttccgggaac ccaacaattg cattggggca tgcctgaccc gtttgatgtg gtggggacgg
     1201 aggaggaaaa gcggaatgcc tatcgcgcgt gccgggatgc tttgcaaaag cgcattcgtg
     1261 agtttgttgg ctagtaaatt ttattatttc atttcttcat ttcttgattg attcattgat
     1321 tcatctcccc aacacacgga tttgcccgac tcggtgatgg cgcaggagtg gctgtctccc
     1381 gatgacacca aaagaaaatt ctctggcggg agtgactttg gcttggcttg cgaattcaaa
     1441 tcactgcccc agcaggcgat gtgtttgtcc aaagtgacac cgcaggtgtg attgcggcct
     1501 gaactgattt gcatgaattg agtgttgggc gcgttgagtt gcttcatcga atggtcgccc
     1561 caacatagcg cattttgatc cgacgtgatc ccgcacatat gataaccgcc agccgagaga
     1621 ctgatgaaat cacgatcagg gacatgcaac tgcgcttctt tgttgcgtcc ccagcacacc
     1681 acatcacctt tggtggtcag tccgcagttg tgatattcgc ccgcgctaat ggtgatgaat
     1741 tcctggggaa catgagtgac ggattgatcc gttgtgattt ctgcctgtgg gacatccgtt
     1801 tgaccttggg agttgttacc ccaacagagc acatgattgt cctcatcgat ggcgcaggcg
     1861 tgttccctgc ctacggacag gtcgatgaat
//
LOCUS       DATDUN010000798         2372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_574713, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000798 DATDUN010000000
VERSION     DATDUN010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574713"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VJC18_11120"
     CDS             <1..1527
                     /locus_tag="VJC18_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLD45975.1"
                     /translation="AKTNQVANYLKEELGVVKNDRVALLFPMLPELLPVFFAVVKLGA
                     IAVPLFLSYSEENMHSICKTLGVTVLFTADGYHERGQKLFLKDRADKIVDACEQMKQC
                     VVIENIGDRTQAMRPDRDVYFADVVGALSAEASTEELSSDHIAAILHSVGPDHQLIPL
                     VHTHGGLLAQVTKEFFLNFDIDSKDVFCWNTNFVDMIALWEIIGTTHFAATVLLAEGA
                     PHPHRPEQIFELCEDHGVTHLGVATGLMRRLQHAGFKIPPQCTLASLRVIGAAGEPWT
                     REAWQWCYENIGRSKLPILTMLCHPSILGSYLLPLVVSDIKNSSLQSPGLGMCVDAYA
                     EAGYALGRGQSGYLVCKKPTPSMSKGFVIDKDLHQLTFFSQFKNVWTQGDWVKCDSDG
                     HWFHQGSAGQTVKEGPDRIGLKDIEEKICRHVFVDKAYVSLGVAVERASGVDCFICLK
                     KGIGLTRFLTENILKLLATRTGELARNSQLHFVPALPYTDEGIVDEEALHVVVGHDRP
                     "
     gene            complement(1548..1733)
                     /locus_tag="VJC18_11125"
     CDS             complement(1548..1733)
                     /locus_tag="VJC18_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45976.1"
                     /translation="MQAILSELSQIKEKLGAVEQSEQELKTNMRMIFADLTHFENLVL
                     KILDHRTPSLETEKYQH"
     gene            1985..>2372
                     /locus_tag="VJC18_11130"
     CDS             1985..>2372
                     /locus_tag="VJC18_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HLD45977.1"
                     /translation="MAQDSAQEKTEHATPKRLREAKKKGDVAKSKDLTTVFVMMAVFA
                     AIAFSIDFMGDVLKDFMKLCIDLTAKKEIVLADVSHVLKMLVLTLAKASAPALLSGVI
                     AALLVGLVQVGGVFSMEPLMPKMEKLN"
BASE COUNT          557 a          506 c          668 g          641 t
ORIGIN      
        1 gccaagacca atcaggtggc caattatctc aaagaagagc ttggtgtggt caaaaacgat
       61 cgtgtggccc ttctgtttcc catgctacca gagctgcttc ccgtattttt tgccgtggtc
      121 aaactgggcg ccatcgccgt tcctcttttt ttatcataca gcgaagagaa catgcactcg
      181 atttgcaaga ctttgggggt gacggttttg tttacggcgg acggttatca tgagcgaggg
      241 cagaagctct ttttgaagga cagggccgat aaaattgtcg atgcgtgcga gcagatgaag
      301 caatgtgtgg tgatcgaaaa catcggtgat cgcacccaag ccatgagacc ggaccgcgat
      361 gtttattttg cggacgttgt gggagccctg tccgcagagg cctctaccga ggaattatcc
      421 agtgaccaca tcgccgccat cctgcattcc gtggggccgg atcaccaact catcccgctg
      481 gtccacacgc atggtggttt gctggcacag gtgaccaagg agttcttttt aaatttcgat
      541 attgattcaa aagacgtgtt ttgttggaac acaaactttg ttgatatgat tgccctgtgg
      601 gaaatcattg gaacgactca ttttgcggcg acagtgcttt tggcggaagg agcgccgcac
      661 ccgcatcggc ccgagcagat ttttgaactc tgtgaagacc acggcgtgac tcacttgggt
      721 gtggcaacag gcctgatgcg ccggttacag catgcaggat tcaagattcc cccacaatgc
      781 acgcttgcca gtttgcgtgt gattggtgcg gctggtgagc cgtggaccag ggaggcctgg
      841 caatggtgtt acgaaaatat tggccggtcg aaattaccga tcctcacgat gttgtgtcat
      901 ccctccatcc tggggagtta tttgttgcca ttggtggttt ccgatatcaa aaattcatcc
      961 ctgcaaagtc ccgggttggg gatgtgtgtg gatgcgtatg ctgaggcagg ctatgccttg
     1021 gggcgaggcc aatcagggta tttggtgtgc aagaaaccga caccgtccat gtcgaagggg
     1081 tttgtgattg acaaagacct tcaccaacta actttttttt cacagttcaa aaatgtgtgg
     1141 acccaaggag attgggtgaa gtgtgacagc gatggtcatt ggtttcatca ggggagcgcg
     1201 gggcaaacgg tcaaagaggg gcccgacagg atagggctta aggacattga agaaaaaatt
     1261 tgtcggcatg tgtttgttga taaagcctat gtttcgctgg gtgtggccgt cgagagggcc
     1321 tctggggttg attgttttat ttgtctcaaa aaagggatag gactcacacg gtttctcacg
     1381 gaaaacatct tgaaactgtt ggcaacgagg acgggtgaac tggccagaaa cagccagctt
     1441 cactttgtgc ctgccttgcc gtataccgac gaaggcattg tggacgaaga ggcgctccac
     1501 gtcgtggtcg gtcatgaccg gccttgatcc ttcgattaga ggacacccta atgctggtat
     1561 ttttcggttt cgagggaggg ggtccggtgg tcaagtatct tcaggaccag attttcgaag
     1621 tgggtgaggt cggcaaagat catcctcatg tttgttttga gttcctgctc cgattgctcc
     1681 acggcgccca gtttttcttt tatttgcgat agttccgaga ggatggcttg caagttcttt
     1741 tccatatggc aaaaatgtaa taattacaaa tagatgaaaa tgataattgc atgttcatct
     1801 gctgtgtagg gagtatcgac aacctcttgt ataaagttgt gtgtttttta cgatagtttt
     1861 gtagaaatta gagtcattta tcaactttcg tgtttatctg gacgataatc ggaatatgtg
     1921 atccactact gacggattga cgagatactg cttttgcaac agcaatagca gaggtgatga
     1981 gcatatggca caggattctg cacaggaaaa aaccgagcat gcgaccccaa aacgcctgcg
     2041 tgaagcaaaa aagaagggcg atgtggccaa aagcaaggac ttgaccacgg tttttgtgat
     2101 gatggccgtg ttcgcggcga tcgctttcag catcgatttc atgggtgatg tgctcaagga
     2161 tttcatgaag ctctgcattg atttgacggc gaaaaaagag attgtcttgg ccgatgtatc
     2221 acatgttctc aaaatgctcg tgttgacatt ggccaaggcc tcggcccccg ctttattgtc
     2281 tggggtgatc gcggccctgc tcgtgggttt ggttcaggtc ggaggggttt tttcgatgga
     2341 acccttaatg cctaagatgg agaagctcaa tc
//
LOCUS       DATDUN010000799         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_242549, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000799 DATDUN010000000
VERSION     DATDUN010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242549"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VJC18_11135"
     CDS             <1..639
                     /locus_tag="VJC18_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45978.1"
                     /translation="QGRQDCLDTVCREAVAVFNHNIETVARLTPLVRSVARYQRSLEV
                     ISYVHKTYPHIRTKSGLMLGLGETRDEVLQSMRDLRAHGCQLLTLGQYLQPTRKHYPV
                     MAHISQEQFTEYENLGREMGFETVFAGTFVRSSYRAGGYTKDEGRKHEGRGTRDEGTR
                     DENTRDEGQAEWTLRQAQGRLKGREKTKIKNEKCKMQNAKCKMQNAKCQPHD"
     gene            701..1750
                     /gene="mqnE"
                     /locus_tag="VJC18_11140"
     CDS             701..1750
                     /gene="mqnE"
                     /locus_tag="VJC18_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HLD45979.1"
                     /translation="MNTDLESIMEHCREGQRLSEAEALLLSEKTDLLTLGRLAHARRE
                     QRFGRNTHYRNNLHIDYSNICTAHCLLCAYARNKGQNGAFTKTIDEILGSIRRYALAI
                     SEVHIVGGHHPDLPYAYYLDLLKNIRREFPKLHIKAFTAIEIHYFALKFGKSVSQILT
                     DFIACGLNSLPGGGAEILVDEVRKKICPQKGASQVWLDVHKTAHKLGLTSTATMLYGH
                     VESLADRVSHLEKIRRLQDETNGFTAFVPLPFHPANTALADIPPVTAHETLKMIALSR
                     IFLDNIPHIKAYWVMLTMPVAQIALGFGADDLHGTVISEHISHSAGATTPQGYSENDL
                     ISAIKEAGGNPVIEN"
     gene            1936..>2742
                     /locus_tag="VJC18_11145"
     CDS             1936..>2742
                     /locus_tag="VJC18_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45980.1"
                     /translation="MAVLQTLPTQFHPSQSYIPPFEIANLLEAERDILGNEQHRMPPE
                     WLPLSRPQATALGDLALAGLTLSETSVRLPDASPEIRRASDRLRTQLNEYQEMMEGRP
                     RASDFRTAPVPVLSAESTQGVDDVMEIYALSLRVRKPHWEIRTIPVEENRVKLVITIP
                     KGEPVYIHDIFGSASSVKDHLVDSVNIDLDTGALHRELFSKKADPDEEPIWAVYSDRI
                     SVVTTFTDEGTQIEVVPAGQLGDDFILDDYDSSLDLREGRIPNSLYFEGVS"
BASE COUNT          720 a          639 c          734 g          649 t
ORIGIN      
        1 cagggacgac aggattgtct ggacacggtc tgtcgggagg ctgtcgctgt gttcaatcac
       61 aacatcgaaa ccgttgcgcg cctgacccca ttggttcgct ctgtggcaag gtatcagcgt
      121 tctctggagg tcatcagcta tgtgcacaag acctaccctc acatcaggac gaagtcgggg
      181 ttgatgctgg gtttggggga gaccagggat gaagtcttgc agagcatgcg tgatttgcgt
      241 gcgcacgggt gtcagctcct cacgctgggg caatacttgc aaccgacccg gaagcactac
      301 ccagtcatgg cccacatttc tcaggaacaa tttaccgaat acgaaaattt aggcagggaa
      361 atgggctttg agaccgtttt tgcaggaacc tttgtgagga gttcgtatag ggcaggggga
      421 tacacgaagg acgagggacg aaaacacgag ggacgaggga cgagggacga ggggacgagg
      481 gacgaaaaca cgagggacga agggcaggct gaatggaccc ttcgacaggc tcagggcagg
      541 ctgaagggac gagaaaaaac aaaaataaaa aatgaaaaat gcaaaatgca aaatgcaaag
      601 tgcaaaatgc aaaatgcaaa atgtcaaccg cacgactgat tctgatttgc tgggaaaata
      661 attgcaacag ttgttgattt ttacgcatgc acgctctcat atgaacacgg acctcgaatc
      721 aatcatggaa cactgtcggg aagggcaacg cctgagcgag gcagaggcat tgttgttgtc
      781 tgaaaaaacc gatttgctca cattgggcag gttggcccat gccagacgtg agcagaggtt
      841 tggtcgaaac acccattacc gtaacaatct gcacattgat tattcgaata tttgcactgc
      901 acattgtctg ctctgcgcgt atgccagaaa taagggacag aacggtgcgt ttacaaaaac
      961 cattgatgag attctgggca gcatcagacg gtacgctttg gctatttccg aggtgcacat
     1021 tgtcggtggg catcatccgg atttgccgta cgcctattat cttgatttgc tcaaaaatat
     1081 tcgccgcgaa tttcccaagt tgcacatcaa ggcgttcacc gccattgaga ttcattattt
     1141 tgcgctgaaa tttgggaaat cggtttcaca gatcctgaca gatttcatcg cgtgtggttt
     1201 gaattccctg cccggtgggg gggcagaaat tttggtggat gaggtcagaa aaaaaatctg
     1261 cccgcaaaaa ggcgcatcgc aagtttggct cgatgtgcac aagacagcgc acaagttggg
     1321 cctcacatcc acagccacca tgctttatgg gcatgtggaa tctctggcgg accgggtgtc
     1381 gcatctggaa aaaatccgcc gtttgcagga tgagaccaac gggtttaccg cctttgttcc
     1441 cctaccattt catcccgcta atactgcctt agcagacatc cctcccgtca ccgcgcacga
     1501 aacactcaag atgattgccc tgtcacgaat atttctcgac aacatccctc acatcaaggc
     1561 gtactgggtg atgctcacca tgccggtggc gcagatagcg ctgggctttg gcgcggacga
     1621 tttgcatggc acggtgattt ctgaacacat ctctcattca gcgggagcca ccacaccgca
     1681 ggggtattcc gaaaatgacc tgatttctgc gatcaaagag gcgggaggga atccagtgat
     1741 cgagaattga tgctacgcac acgatgtcgg ccgcatacct gcaatttttt cgtccacaat
     1801 tgtggactga attttcatgc ccccattgtt tttctaccaa caaatcaact aagaagccaa
     1861 ggtgtctgtt gtgttgtgga taggctgggt ctgttcggga aaaccagacg ataacaaaat
     1921 tcgcgggagg gcatcatggc agtattacaa actttaccaa ctcagtttca tcccagtcaa
     1981 tcatacattc cgccctttga gatagccaat ttgttagagg cagaacgtga tattctggga
     2041 aacgaacagc atagaatgcc acccgaatgg ttgcccttgt cccgtccgca ggctactgcc
     2101 ctcggagatc ttgccttagc tggcctgacg ttatccgaga ccagcgtcag gctccccgat
     2161 gcgagccccg aaattcgcag ggcgagtgac agactcagga cacaattgaa tgaatatcag
     2221 gagatgatgg aggggcgacc aagggcttcg gattttcgca ctgcccctgt gcctgtgttg
     2281 tccgcagaat cgactcaggg ggtagacgat gtgatggaga tttacgccct ttccctgaga
     2341 gtgaggaagc cccactggga gattcgaaca atccccgtag aagaaaatcg ggttaaatta
     2401 gtcattacca taccaaaggg cgagccggtt tatattcatg atatttttgg ttcggcaagt
     2461 tctgtcaaag atcatctggt ggactctgtc aacattgact tggatacagg ggcccttcat
     2521 cgagaacttt tctctaagaa agctgatccc gatgaagaac caatatgggc cgtttatagt
     2581 gataggatta gtgtcgtcac gacatttacc gatgagggca cacaaatcga agttgtgcct
     2641 gcggggcaac taggtgatga ttttatcttg gatgattatg attctagctt ggacttgcga
     2701 gagggcagga tccccaattc tctgtatttt gaaggggtaa gt
//
LOCUS       DATDUN010000800         1113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_82578, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000800 DATDUN010000000
VERSION     DATDUN010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82578"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VJC18_11150"
     CDS             <1..764
                     /locus_tag="VJC18_11150"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HLD45981.1"
                     /translation="PGHADLCGGLKYEQHDLRDVLERASARETAIRTAVGAVCRQFLA
                     AFDIGASSQIISIGGETKNTGARIARAEQEGDSVGGLFEVRFTGVPVGLGSHTHWDEK
                     LSANLARTLMSMQAIKGVAFGLGFGLADLPGSKAHDPIVYNKSKKQFTRLTNNAGGFE
                     GGMTTGEPIIIRAVMKPIATLIKPLGTINIKTKKSTLASVERADTCAVHSASVVAENI
                     LCVDLANAFLQKFGGDSLQETSRNYRGYLKQIKDY"
     gene            complement(778..972)
                     /locus_tag="VJC18_11155"
     CDS             complement(778..972)
                     /locus_tag="VJC18_11155"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HLD45982.1"
                     /translation="MEFNGQPSTDSKEVSDDVVVDLDNLGNIVGIDIQNASTKLDLKT
                     LETTALPVISSRFQATEDRA"
BASE COUNT          286 a          282 c          300 g          245 t
ORIGIN      
        1 gtccaggtca cgcggatctc tgcggcgggc tcaaatacga gcaacacgat ttgcgcgacg
       61 tattggagcg cgcctccgcc cgcgaaactg ccatccgcac cgctgtgggg gcagtgtgtc
      121 ggcagttttt ggccgcgttt gacattggcg caagcagtca gatcatcagc attggcggcg
      181 aaacaaagaa caccggggcc cgcattgcgc gtgccgaaca ggaaggcgat tcggtgggcg
      241 gcctcttcga ggtgcgtttc acgggcgtcc ctgtggggct ggggtcgcac acccattggg
      301 acgagaagct ctctgccaat ctggcgcgaa cactcatgtc catgcaggcc atcaagggcg
      361 tggcatttgg gttgggtttt ggtttggcgg atttgccggg atcaaaggca cacgatccaa
      421 tcgtgtacaa taaatccaaa aaacaattca cgcggctcac caacaacgct ggcggttttg
      481 aggggggcat gaccacgggt gaacccatca tcatccgtgc ggtcatgaag cccatcgcca
      541 cactcataaa acctctgggc accatcaaca tcaaaaccaa aaagagcaca ctcgcctctg
      601 tggaacgtgc cgacacctgc gcggtacact ctgcaagcgt ggtggccgaa aacattttgt
      661 gtgttgatct ggccaacgcc tttctgcaaa aatttggcgg cgattccctg caggaaacga
      721 gtcgaaatta tcgggggtat ctaaagcaga tcaaggacta ttgaatcaga aaagtgacta
      781 cgccctgtct tccgttgcct gaaaacgtga tgaaataacg ggaagtgccg tggtctctaa
      841 tgtttttaga tcaagttttg ttgacgcatt ttgaatgtca attccaacaa tatttcccag
      901 attatccaaa tccacaacaa catcgtcgga cacttctttg gaatcagtac tcggttggcc
      961 gttaaattct atagggggcc tctaaaaact atgttcgtta cgaaaaacca tgattcgtag
     1021 ccgagacaag gaagatgagg aaaaagcccg gaaacgtata aggcacgcta cgttgaggac
     1081 tttttgcgaa atctgacaaa gtcgcaggcc gaa
//
LOCUS       DATDUN010000801         1294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_533742, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000801 DATDUN010000000
VERSION     DATDUN010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533742"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VJC18_11160"
     CDS             <1..409
                     /locus_tag="VJC18_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HLD45983.1"
                     /translation="LQELVCEDLNLRSRIVSIGIPILLSCGKKLLRGQHMAIPADVPG
                     NVMAQFEITDARLQHWAHDGWVDLRPDNMAVWQKRLHYIKDMIDSIPEDDSSSCFAKG
                     EQYWEVQGGDIPLSLAKLASWVFTHEDQGKRMK"
     gene            complement(637..1029)
                     /gene="rpsI"
                     /locus_tag="VJC18_11165"
     CDS             complement(637..1029)
                     /gene="rpsI"
                     /locus_tag="VJC18_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HLD45984.1"
                     /translation="MIRKRFYGTGRRKNSIARLWLTPGTGGFEANGRNMETYFPRETL
                     RMMILQPMEATGNIGKFDVIANIHGGGIAGQAGAVRHALARALLQLNQDYRKPLNRGG
                     FLTRDSREVERKKYGHRKARKRCQFSKR"
     gene            complement(1106..>1294)
                     /locus_tag="VJC18_11170"
     CDS             complement(1106..>1294)
                     /locus_tag="VJC18_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HLD45985.1"
                     /translation="MGGLREINAKKLLDKDPGHIITAAVKGMLPRGPLGRKQLKKLKV
                     YAGSEHPHSAQKPLVIEL"
BASE COUNT          290 a          302 c          355 g          347 t
ORIGIN      
        1 cttgcaggag ttggtttgcg aagatttgaa tttgcggtca cgtattgtct ccattggtat
       61 tcccattttg ttgtcttgcg gcaagaaact gttacgtgga cagcacatgg ctattcccgc
      121 tgatgtgccg ggcaatgtga tggctcagtt cgagatcacc gatgcacgcc ttcagcactg
      181 ggcgcatgat ggctgggttg acttgagacc tgataacatg gctgtctggc agaagagatt
      241 gcactacata aaagacatga tcgactctat tcccgaagac gacagtagct cttgctttgc
      301 caaaggggaa cagtactggg aagtacaggg gggtgacatc cccttgtcac tcgccaagtt
      361 ggcgagttgg gtgtttaccc acgaggatca ggggaaacgg atgaaataaa aaaacctcca
      421 aaaacagccc atctgcggtg ttgtccacaa aattgcaatc ctcacgtatt tacgtatacg
      481 ctcagtttgc aattttgtgg acgcctagca tctggacctt ttttggaggt ttttgaatat
      541 tgaatcttgc aataatgcca aaaaaaagcc gaacaggggg ccagttcggc ttttttgaat
      601 aatgttcgcg ccaacaaggt ggcggctgaa accagcttaa cgtttggaaa actggcatct
      661 cttgcgggct ttgcggtggc cgtatttctt gcgttccact tcgcgggaat cgcgggtgag
      721 aaaaccacca cggttaagag gcttgcggta atcctgattg agttgcaaca aggccctagc
      781 caaggcgtga cgcacggcac ctgcttgtcc ggcgatgccg ccgccgtgaa tattggcgat
      841 cacgtcgaac ttgccgatgt tgcccgtggc ttccatgggc tgtaaaatca tcatgcgcag
      901 tgtttcgcga gggaagtagg tttccatgtt gcggccgttg gcctcaaaac cacctgtgcc
      961 aggtgtgagc cagaggcgag cgatggaatt ttttcttctg ccggttccgt aaaatctttt
     1021 tcgtatcatg gtcatgatta atgagttatg ggcgacgggc gatgagtttg ataaacccaa
     1081 gacccctcac gcacgacgtg ctgaattata attcgatcac cagcggcttt tgagcactgt
     1141 gtgggtgctc ggaaccggcg tagaccttaa gttttttgag ttgtttgcgt cccaacggcc
     1201 ccctgggcaa catgcctttg acggcggctg tgatgatgtg gccgggatct ttgtcgagca
     1261 gttttttggc attgatctct ctcagtcctc ccat
//
LOCUS       DATDUN010000802         1586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_618204, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000802 DATDUN010000000
VERSION     DATDUN010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618204"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VJC18_11175"
     CDS             <1..1086
                     /locus_tag="VJC18_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45986.1"
                     /translation="QNPARISHHTFALLGDPDIAKQIEAATTHDMSGMNRSIYVDPEA
                     LMGKQQARSVSRDSVYRHLTGIGAIRQRHPRLAFKWDQHVTLAEWQGAPDPAFRQHAC
                     LSRSLDIGLVASSRTEMLFGPGVTLRDLVSVFDPDCEDCLLAEFSLVEHKLDYWHLQI
                     DARYEDHLEIPMGTRHVSYAIRQGKKYQTPMMCRESCYIHFGKNRGTGIGSHLFMKSL
                     AFDHRAPHVQEARIKAMNVGRYAWRHLPLILTADDRKAIQDILSYINDQLDLHLSPQK
                     IDAIRSPRDVSGICTGRKVDRRVLDFVQMVATHWQDHMGLEDHHFTLTPRQIQYIVKH
                     PGILALMMMPNYDMGWKPHNKAHITLS"
     gene            1367..>1586
                     /locus_tag="VJC18_11180"
     CDS             1367..>1586
                     /locus_tag="VJC18_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HLD45987.1"
                     /translation="MKQLTPEQIRTMTLEEKDQWWLENVWRGDMPQLTLRSALTGMVL
                     AAVLSLVNLYICIMTGWNVGVGIISVIIS"
BASE COUNT          427 a          391 c          375 g          393 t
ORIGIN      
        1 caaaacccag ccagaatttc acatcatacc tttgctcttt tgggtgatcc tgatatcgcc
       61 aaacagattg aggccgcaac aacccatgac atgtccggaa tgaatcggtc catctatgtc
      121 gatcctgagg cgttgatggg aaagcaacaa gcaagaagcg tctcacgaga cagcgtgtac
      181 agacacctca ctggcatcgg agctatccgc caaagacacc cacgccttgc gttcaaatgg
      241 gaccaacacg tcactctggc agaatggcaa ggtgctcccg atccggcttt taggcagcac
      301 gcctgtttgt cgcggtcact tgatataggg cttgttgcct cctctcgtac agagatgttg
      361 tttggacccg gcgtaacctt gcgggatctg gtcagcgtat tcgaccccga ttgtgaagat
      421 tgcctgctcg cagaattctc acttgtggaa cacaagctcg attattggca cctgcagatt
      481 gatgcccgct atgaggacca tttggaaatc cctatgggca ccagacacgt cagttatgcc
      541 atacgacagg gcaagaaata ccagaccccc atgatgtgcc gtgaatcatg ttatattcac
      601 tttggcaaga atagggggac agggattgga tcacaccttt ttatgaagag tctcgccttt
      661 gatcataggg ccccgcatgt tcaggaggca cgaatcaaag cgatgaatgt aggacgttat
      721 gcctggagac atctgccttt gatcctcacc gcagatgacc gtaaagccat ccaagacatc
      781 ttgtcataca tcaatgacca gctggatttg cacttgagtc cacagaaaat tgacgccatc
      841 agatcaccac gtgatgtcag cggcatttgc actggtagaa aagttgacag gcgcgttctt
      901 gattttgtgc aaatggtggc cactcattgg caggatcata tgggactgga ggatcaccat
      961 ttcactctca cgccaagaca aatacagtac attgtcaaac acccgggaat cctggccctg
     1021 atgatgatgc ccaactatga catgggctgg aaacctcaca acaaagcaca cataacatta
     1081 tcgtgaacgg atcccgtaag atttgtggtt cagtgtggct cggcaactcc gtcatacaat
     1141 cttattaagt ttcctaacga gaaccattag gcaaaataac cgtgtcctgt gggacacgat
     1201 attttattta aaacacacac catttgccga atccatgccg acaagtggtc agtggcgtgc
     1261 ccacaacgcc aattggatca catccgacaa tctgctttaa ttttttgatg ttttatgatt
     1321 tagttatcaa atgtttttta actctcgatg aagtggagat gaaacaatga aacaactgac
     1381 acccgaacag attcgaacga tgaccttgga agaaaaagat caatggtggt tagagaacgt
     1441 gtggcgggga gatatgcccc aattaaccct gcgttcggca ctgaccggca tggtcttggc
     1501 ggcggtcctt tccttggtca atctgtacat ctgcatcatg acaggctgga atgtgggagt
     1561 gggaatcatc tccgtcatca tttcct
//
LOCUS       DATDUN010000803         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_533920, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000803 DATDUN010000000
VERSION     DATDUN010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533920"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..311)
                     /locus_tag="VJC18_11185"
     CDS             complement(90..311)
                     /locus_tag="VJC18_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45988.1"
                     /translation="MNHEFPFEEMASLENSAVCKEVFADIKKFVKDRTSPAVTDVVRT
                     LKEIGIHEDPRMILNMMNMIANSGGKKMG"
     gene            complement(301..1020)
                     /locus_tag="VJC18_11190"
     CDS             complement(301..1020)
                     /locus_tag="VJC18_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45989.1"
                     /translation="MNNSQSMIRKRVVNLLNGRAGYIVRRMISSPDQHWSGHELAKEL
                     CMSPAWVNRVLDNLVAFGVVKRTQRGVLSFTEVVSNNIIIEKWVSHYDFNMNPTRFYL
                     LQKKNPVKLIDALAEKEGFSYALTGYAAANLVEKTTYNGVPSVYLVPKSGFNSDEFNN
                     ILGRLENVYNFIPVNKQANVIVMQPLQREAVLFDVKKIRGINCVSPLQLYLDLYGLDR
                     GKFVIEALNDYFQKHKLFYES"
     gene            complement(1307..>2080)
                     /locus_tag="VJC18_11195"
     CDS             complement(1307..>2080)
                     /locus_tag="VJC18_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HLD45990.1"
                     /translation="GDGDFDRPLLYTETGEALYGDRLGIMAALELKRITGTKVVVVTA
                     LSSSTALEKTGAFEKVVLTKIGSPYVVSAMYREMEKADDDTIVVGYERNGGFFLGTPV
                     QGVGGTLEPLITRDAVLPMLLALNAVKERAMTLSAVHNELPPALTASQGFVFPREKSQ
                     AILARFRSGTFEDDARAVMAAFGTALFSDEDIHVVAINTEDGVRITFSSGEIVHLRPS
                     GNEPKLRVYTEATTPERASAMMSLALDALRGLAENMLVT"
BASE COUNT          568 a          533 c          422 g          557 t
ORIGIN      
        1 agatcgtgat caatctgaat attacaccaa ttcccctcgc cagagctggg cataaaattc
       61 catccagggc agcaccagaa tatcgttgat caccccattt tttttccacc agaatttgcg
      121 atcatgttca tcatattcaa aatcatcctg ggatcctcat gaataccaat ctctttcagt
      181 gttctgacaa catcagtcac agcaggggat gttctgtctt tcacaaactt tttgatgtct
      241 gcaaagacct ccttacacac agcactattt tccaaggagg ccatttcctc aaaagggaat
      301 tcatgattca taaaatagtt tgtgcttctg aaaatagtcg ttcaaggcct cgataacaaa
      361 tttgccacga tccaaaccat aaagatccag atacagctgt aggggactca cacaattaat
      421 tccacgaatt ttttttacat caaaaagaac tgcttcccgt tgtaacggct gcatcacaat
      481 cacattggct tgtttgttga ccggaataaa attgtagaca ttttcaagtc tgccaagaat
      541 attattgaac tcatcgctgt taaaacccga ttttggaaca agataaactg acggaacacc
      601 attgtacgtt gttttttcaa ccaagttggc tgcggcatat cccgttagag cataagaaaa
      661 tccctccttc tctgccaatg cgtcaatgag cttaacggga ttctttttct gcaaaaggta
      721 gaacctggtg ggattcatgt tgaaatcata atggctaacc catttttcga tgatgatgtt
      781 gttgctcaca acctcagtaa aactcaggac ccccctttga gtccgtttaa caacgccaaa
      841 ggcaaccagg ttgtctaaaa ccctgttgac ccaggcagga ctcatacaga gttctttggc
      901 aagctcgtga ccactccaat gttggtctgg cgaactgatc attcttctga cgatatatcc
      961 cgcgcgaccg ttcaacaaat taacaaccct ttttctgatc attgattggc tattattcat
     1021 aattggctac tattttgtat atattataat atagtagcca ttcgattgta aagacaaaaa
     1081 aacaagcaaa caaactggcg gccgatcgat ggtaaaatat tttattgtga cgcttaagac
     1141 cgcttaaagt tgttagtttc agctccccat caccggccct gatggaaagc ggatactttc
     1201 atcacattgt aacagccgat cagttgttgc ggtttgaata ttgactaagc ggattattct
     1261 ttttcgaaac tgctcaacac cagataaaat ttaaattgtc gaaggactag gttacaagca
     1321 tgttctctgc caacccccta agcgcgtcga gggcgagcga catcatcgcg gaggccctct
     1381 ccggtgtggt ggcctcggtg tacaccctga gttttggctc attgccggag ggtctcaagt
     1441 gcacaatctc gccactgcta aaggtgatgc gcacaccatc ctcagtgttg atagcgacca
     1501 catgaatgtc ttcatcggaa aacaacgctg tcccaaaagc ggccatgaca gcccgggcat
     1561 cgtcctcaaa tgttcccgaa cgaaaacggg cgaggatcgc ctgacttttt tccctgggaa
     1621 aaacaaatcc ctgactggcc gttaaggctg gcggcaactc gttatgcacg gcagataatg
     1681 tcattgccct ctctttaacg gcattcaagg ccaacagcat ggggaggacc gcgtcgcggg
     1741 tgatcaaggg ttctagcgta ccccctacac cctgaacggg tgtgcccaaa aagaatccac
     1801 cgtttcgttc gtaacccaca acgatagtgt catcgtcagc cttctccatt tcacgataca
     1861 tggcgctcac cacatagggc gacccgatct tggtgaggac aactttttcg aaggcccccg
     1921 ttttttccag agccgtgcta ctgctgagcg ctgtaaccac cacaaccttt gttcctgtga
     1981 tgcgtttgag ctccaacgcg gccatgatcc ccagccgatc accatacaga gcttcacctg
     2041 tctcggtata caaaagcggt cgatcaaaat caccgtcacc
//
LOCUS       DATDUN010000804         3006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_82839, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000804 DATDUN010000000
VERSION     DATDUN010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82839"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..489)
                     /locus_tag="VJC18_11200"
     CDS             complement(43..489)
                     /locus_tag="VJC18_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45991.1"
                     /translation="MRSLRYLLTFACSMFCFLGMGQPAMGSVCEVIPATLPTDQRHVI
                     TLECEINRELLYYSYQMITTTGIRGEYPVYLDASMLGTCTAWNPCFTNLRGVLRRPDP
                     SLPGAYLTGITDGSWTKVDSVTYQHISSNTTCAYDNTLGTFLCDMF"
     gene            963..1934
                     /locus_tag="VJC18_11205"
     CDS             963..1934
                     /locus_tag="VJC18_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD45992.1"
                     /translation="MAHVTAGALVPEVMWRSARRLAAVTPQNLTPVSLRRGPAQTHPA
                     GAPSQLVIRSATGAAVVSTCESAPVIAKGKSTERLFPSDGPDFSLTVPTPMPKAVAVN
                     SPVQVPTIMATTKTDPVDSVQPQRVNHSTQTAGTGVPTQYNGLIGTLDALIADLIDVE
                     TARVPGLTGTTEADDYAVNLLLGQMTRQPLVWALQTLGEYVYRTDLLTQPTHVDRVKS
                     ALSLLGFEQSVVTKAMRSIAVENFAGFISTEILSSQGRAVILDRVRIAPSQTVVTAGS
                     NGLTLRDVLGRQGTGADWHLREEDVPDTDLRVEESSASGNLSNQTRQ"
     gene            2353..>3006
                     /locus_tag="VJC18_11210"
     CDS             2353..>3006
                     /locus_tag="VJC18_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLD45993.1"
                     /translation="MKPAHPFNKRLKTVSAKVLKLIAQIDELKGRWSSGARLSPQVLG
                     RLKVSVLVTSTGSSTRIEGARMSDGDIEKMLRGLDIQRFKDRDKQEVRGYYDLLKNVF
                     EAWEKIKLTEGNIKFFHKELLKHTEKDQSHCGHYKKRENKVHMIDTAGQSLGVLFDTT
                     AAYLTPKEMLELVEWTQEALKENTHHPLLVVANFVVQFLKIHPFQDGNGRLSRVLTNL
                     "
BASE COUNT          812 a          704 c          708 g          782 t
ORIGIN      
        1 atttttacgg tgtgatcgcc agcaactgtc aaaggccgcc cctcaaaaca tatcacaaag
       61 aaaggttccc agagtgttgt cgtaagcaca cgtggtgttg gaactgatgt gttgataggt
      121 cacagaatcc accttggtcc aagaaccgtc ggtgattccc gtcagatagg cgcccggcaa
      181 actggggtct ggcctgcgca atacacctct caggtttgta aagcagggat tccaagcggt
      241 acatgttcct aacatactgg catcgagata cacagggtat tctcccctga tgcctgtcgt
      301 tgtgatcatt tggtatgaat aatacaaaag ttcacgattg atttcacatt caagtgtgat
      361 gacgtgacgc tggtcggttg gcagagtggc cggaatgacc tcacagacgg aacccatggc
      421 tggttgtccc atacccaaga agcagaacat gctacaggca aatgtcagta aatatctgag
      481 tgatctcata gattacctcc ggtggctgtc ataataaaga tcaaaaaaag gcgcaacttt
      541 ttgtaaactc ctccggactg ccctgcggaa atgattttgg tgtggctcag gtcatccata
      601 gaggctctaa tggccatttg gtaacatccc ctgctttgtc ctgatcacac tttgtcgctt
      661 tttcaacaga ctgctgacaa gcccgtcacg accctgacaa tttttattac aatttttatt
      721 gcattttatt ggcggttatt actaagctct agctcatgcc aatacaattt agatcaaatc
      781 cctttttgtt gcagggggcc tcgaccatat tgggttttgt tcctgtctcg cctacagtcc
      841 atcagagaca accccaacga actattattc cccccacatt tcggcggaca attattcctg
      901 atctcaagcc cgcattggtg ggcttacccg tggcccgacc cagtcgtccg caatgggtac
      961 caatggcaca cgtgacggct ggggctctgg ttcccgaagt tatgtggcgt tcggcacgta
     1021 gattggccgc agtcaccccg caaaacttga cacctgtctc attgcgaaga gggcctgcac
     1081 agacacatcc agcaggtgct ccttcacaat tggtgatacg ttcggcaaca ggtgctgcgg
     1141 ttgtttcgac gtgtgagtcc gcacctgtga ttgccaaagg caaaagtaca gaaaggcttt
     1201 ttcccagtga tggaccggat ttttcattga ctgttcccac accgatgccc aaagctgttg
     1261 ccgtgaattc gcctgttcag gtgcccacaa tcatggcgac tacaaaaact gatccggtcg
     1321 attctgtcca gccgcaacgg gtcaatcatt caacgcagac ggcgggaacg ggtgtgccaa
     1381 ctcagtataa cggactcatc ggcacactgg acgccctgat tgccgatttg atcgatgttg
     1441 agactgcgcg tgtgcccggt ttgacgggaa caaccgaggc ggatgattat gccgtgaatc
     1501 tgttgctggg gcaaatgact cggcaaccac tggtttgggc actgcaaaca cttggcgaat
     1561 atgtttatag aacggacttg ttgactcagc caactcatgt tgatcgtgtg aaatccgcgt
     1621 tgtctctttt gggttttgag caaagtgttg tcaccaaagc catgaggtct atcgctgttg
     1681 agaattttgc cggttttata tcgacagaaa tcttgagttc acaaggtcgg gctgttattc
     1741 ttgaccgtgt gcggatagcc ccgtcgcaga ccgtggtcac agcgggatca aacggattga
     1801 ccttgcgtga tgttttggga agacagggaa caggcgccga ttggcacttg cgcgaagaag
     1861 acgtgcccga taccgattta cgtgtcgagg agtcgtcggc gtctggcaat ctttccaatc
     1921 aaacgagaca gtagggcttc gaaaaaacag tacatatacc gcgttgtcca tcagaaaaaa
     1981 acataataaa aacaatagtt cagatttatt catggcaata tatgccatac aaggttcaca
     2041 gataccccct gtatcttgcg gcgcggtttc cgctgtgatt tcagatcttc ttgattgttg
     2101 tgccgaagtc atttcaacca gattcaatcc aaagagatgg gatgcgcggc agttgacggt
     2161 cgaaaccaaa cccgtttgca ggataaaatc aaagtcagaa taatagtctg cataaactta
     2221 tacaatctta tatagttaga ttttacaatg aacagcacaa aaataatgaa cttgtattat
     2281 catcatgtta taaaataact ctgcagaaac aaggcttgtt tttagttata taatcttata
     2341 taatcattat atatgaaacc agcacaccct tttaataaaa gactgaaaac agtttcggca
     2401 aaggtcctaa agttgattgc tcaaattgac gaactcaaag ggcgttggtc ttccggggct
     2461 cgtttgagcc ctcaggtttt gggacgtctc aaggtctcag ttttggtgac gtccacagga
     2521 tcgtccacac gaattgaggg tgcccgcatg tccgatgggg atatagaaaa aatgctccgg
     2581 ggactggaca ttcaaaggtt caaagacagg gacaaacaag aggtgcgggg ttattacgat
     2641 ttactgaaaa acgtttttga ggcttgggaa aaaatcaaac tcactgaggg aaacatcaaa
     2701 ttttttcaca aagaacttct caaacacacc gaaaaggacc agtctcattg tggccattac
     2761 aaaaaacggg aaaacaaagt tcacatgatt gacactgctg gtcagtctct gggtgttcta
     2821 ttcgacacca cagcagccta tttaacgccc aaagaaatgt tggaactagt agaatggacg
     2881 caagaagccc ttaaagaaaa cacacaccat cccttgcttg tggtggcaaa ttttgtggtc
     2941 cagttcttaa aaatccatcc ttttcaggat ggcaacggtc gcctctcgcg cgtactcacc
     3001 aacctt
//
LOCUS       DATDUN010000805         3847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_409196, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000805 DATDUN010000000
VERSION     DATDUN010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409196"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VJC18_11215"
     CDS             complement(<1..980)
                     /locus_tag="VJC18_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD45994.1"
                     /translation="MLTEKQKPVSFVGRQYEQAGLLQEFEKPRASLVVIHGRRRVGKT
                     RLLHEFCRDKNTLWVEGLEKGTPQGQRTHFIKQLAEQTIEPFLSRTSSADWTEAFDCL
                     KELLNVLKKSKMESGKMILVFDEFPWLINQDEKIVALFKYYWDNFFKEHNLMIILCGS
                     INTFMVKKVIQSSALYGRLHHEIRLDPLTPKELSLFLGSALPKKDVVEYTMIFGGIPR
                     YWEEINPQESVVQNINRLCFLKDAFFVREFARLFREEFGSINIYTAIVLALSKTSSLN
                     YTEILKQLKTSVGGGYQEYLDNLSLASFVRAFTPLNKKKTRLVRYRLWDE"
     gene            1313..2398
                     /gene="mnmH"
                     /locus_tag="VJC18_11220"
     CDS             1313..2398
                     /gene="mnmH"
                     /locus_tag="VJC18_11220"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HLD45995.1"
                     /translation="MPHKLFRAVFLNNTPLLDVRSPGEFAQGAFPQAINIPLLNDEER
                     AQVGTCYAQKGQTAAIALGHNLVSGEVKKNRMALWIDFATKNPQTLIYCARGGLRSQI
                     VQTWLQDAGISLPRIEGGYKALRGYLLEQIDHISPRLPLIVLGGFTGSGKTRLLKQCA
                     HHIDLEALANHRGSAFGSQFTAQPTSQNFENSLAIRLIKLSRKDPAQLLLEDESHLIG
                     KLLIPPVLFYRMSESPLLVLDTPIEERARIVLGEYVIDEWTTRYQHLPNGHNELALML
                     KTKLKKISKKLGGALFNEIAGDIDKATEQHMSASTFDSHLVWTQKLLTRYYDPMYAHH
                     LGKNQSRVIYRGQTNDILTKILTQTLE"
     gene            2549..3718
                     /locus_tag="VJC18_11225"
     CDS             2549..3718
                     /locus_tag="VJC18_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD45996.1"
                     /translation="MIQRLLTTSNNNSFFLFGPRGTGKTTWLKDRFAKEAPLWIDLLD
                     KDVEEKLLLDPSRLAQEIAAMKSSKEWVVIDEIQKAPKLLDLVHKQIEENNIKFALTG
                     SSARKLKRGAANLLAGRAFLNYLHPLTFRETQNTWPLEDIMRWGSLPKVLNLSSQEEK
                     KHFLKTYTQVYLSEEIVAEQLVRNLTPFRRFLPVAAQGNGQILNYANVSRDIGVDIKT
                     VEAYYEILEDTLMGFRLYPYLSSFRKRLSHKPKFYFFDPGIVRALTRSLDIPLQPRTS
                     GYGDIFEHVVILEFYRLSSYFRSDYELAFIKTKDDAEVDLVIDKPSHPPILIEIKSKT
                     MIQEKDISSLQRLARDIKGSRAYCLSQDPSPQIIGTVSCLPWEKGICEILELPSS"
BASE COUNT         1081 a          943 c          842 g          981 t
ORIGIN      
        1 aactcatccc atagacgata ccgtaccagc cgggtttttt tcttattcaa gggggtgaac
       61 gccctcacaa agctggccaa agaaagattg tccagatact cctgatatcc tccccccacg
      121 cttgtcttga gttgtttgag gatctccgtg taattgaggg acgaggtctt tgacagggcc
      181 aggacgatag cggtgtagat gttgatactg ccaaactcct cccgaaagag acgagcaaac
      241 tctcttacga aaaaagcgtc tttcagaaaa caaagccggt tgatattctg cacgaccgat
      301 tcttgcggat tgatttcttc ccagtaacgg ggaatgccac caaaaatcat tgtgtactca
      361 acgacgtctt ttttgggcaa tgctgagccc aaaaacaaac tcaattcttt gggggtgagc
      421 gggtcgaggc ggatttcatg atgaagacgg ccatagaggg ccgatgactg gatcaccttt
      481 ttgaccatga aggtgttgat ggaaccgcac aaaatgatca tcagattgtg ttctttgaaa
      541 aaattgtccc aataatattt gaacaaggcc acgatctttt cgtcttggtt gatgagccac
      601 ggaaattcat caaagaccaa aatcattttt ccggattcca tttttgattt ttttagaacg
      661 ttcaaaagtt ctttcagaca gtcgaaggcc tcggtccaat ctgccgagga agttctggac
      721 agaaaaggct caatagtttg ttcggcaagc tgtttgatga aatgtgttct ttgtccttgc
      781 ggggttcctt tttcaagtcc ttcgacccag agggtatttt tgtccctgca gaattcgtgc
      841 aagaggcgtg tttttcccac cctcctgcgg ccatgaatga ctaccagaga agcccggggt
      901 ttctcaaatt cctgcaacaa acctgcttgt tcatattgcc gcccaacaaa agacactggt
      961 ttttgttttt cagttaacat aatttattgt atcttaactg aaaaaatact caatcagttt
     1021 ttttcagtta gtacatattt tttactgtta actgaactgt ctgtgctcaa atccactaaa
     1081 acataatttg ttgttcatga aagcctccaa gtaactgctg gaacaaaaag agaccgaggc
     1141 aaaacgtatc gatcaagaag ggaaacggaa cctgccgcaa tatttcatgt tttcacctac
     1201 tcgaaagatg ccgtaagacg ggctacaggc tctcgctcgc ctatgacctg gccctcagcc
     1261 tgaatggcaa aaaatttctt acaagacatc agcttttttg ctaatcacaa tcatgccaca
     1321 caaacttttt agagctgtct ttctcaataa caccccgctc ctcgatgtgc gctctcccgg
     1381 ggaatttgcg caaggggcct tcccgcaggc catcaatatc cctcttttga atgacgaaga
     1441 aagggcccaa gtgggcacct gctatgccca aaagggacaa acagccgcga tcgcgttggg
     1501 gcataatttg gtatcgggcg aggtcaaaaa aaatagaatg gctttgtgga ttgatttcgc
     1561 aaccaaaaac ccgcaaacat tgatttactg cgctcgaggg ggactcagat cacaaatagt
     1621 gcaaacgtgg ttgcaagacg ccggcatcag cttaccccgc atcgagggcg gttacaaggc
     1681 cctgcgaggg tatcttctgg aacaaattga ccacatcagc cctcgcctgc cgctgattgt
     1741 gctcggagga tttacaggtt ctggcaaaac ccgtctgctc aaacaatgcg cccaccacat
     1801 tgatctggaa gcgttggcca atcaccgtgg ttcggccttt ggctcacaat tcaccgcgca
     1861 acccacatcc cagaactttg aaaactcgct cgccataaga ctcatcaaat tgtcccgcaa
     1921 ggatccagcg caactgctgc ttgaagatga aagccatctc attggcaaac tgttgatccc
     1981 gccggtgctg ttttaccgga tgtcagagtc ccctctgctt gttctcgata caccgattga
     2041 ggaacgagcc cgcattgtcc ttggcgaata cgtgatcgat gaatggacca cgcgttacca
     2101 acacctcccc aacggtcata atgaactggc cctcatgctc aaaaccaaac tcaaaaaaat
     2161 cagcaaaaag ctgggaggcg ctctgtttaa tgaaatcgca ggagacatcg acaaggccac
     2221 ggagcaacac atgagtgcgt ctacatttga cagtcatctg gtgtggactc aaaaactgct
     2281 gacccgctat tacgacccca tgtacgcgca tcatttgggc aagaatcaat cacgtgtcat
     2341 ttacagggga cagacaaatg atattctcac aaaaatcctg actcaaaccc ttgagtgatt
     2401 ttacacttca gcaggacatt gccctcatcc gcggcgcggg aactccactc ttcagaacgc
     2461 tggcggggat gacggtgggg tttggaaagt agaaatttca aaatttacta atggacacaa
     2521 ctccaataat ggcgtacaat attggagcat gatccaaaga cttctaacca catcaaataa
     2581 caacagtttt tttctgtttg gtcctcgcgg cacaggcaaa acaacctggc ttaaggatcg
     2641 ttttgcgaaa gaagcccctc tttggattga tctacttgat aaggatgttg aggaaaaatt
     2701 gcttttggat ccatcacgct tagcccagga aatagccgcg atgaagtcgt ctaaagaatg
     2761 ggttgtcatc gatgaaattc aaaaagctcc aaaattgctg gatctggttc ataaacaaat
     2821 cgaagagaac aacatcaaat ttgcgctgac aggatcaagc gccagaaagc tcaagcgtgg
     2881 tgccgctaat cttctggcag gcagagcttt tttgaattat ctgcacccac tgacctttcg
     2941 tgaaacacaa aatacttggc ctctggaaga tatcatgcga tgggggagtt tgcccaaagt
     3001 attgaatctc tcttcgcaag aagaaaaaaa acattttctc aaaacctaca ctcaagtcta
     3061 cctctcagaa gagatcgtgg cagaacagct tgtaagaaat ctcacgccct tcagacgttt
     3121 tttgccggtc gccgcacagg gaaacggcca gattcttaat tacgcgaatg tctcccgtga
     3181 catcggtgtc gacatcaaaa cggtcgaagc ctactacgag attcttgaag atacgttgat
     3241 gggctttcgg ctttacccct acctctcgtc gttcagaaaa cgcctgagtc ataaaccaaa
     3301 attctatttt tttgatccgg gaatagtccg agctcttacc agatctctgg acatcccgct
     3361 tcaaccaaga acatctggtt acggtgacat ttttgagcat gtcgtcattc ttgaattcta
     3421 cagactttct tcatattttc gttccgatta tgaactcgct tttatcaaaa caaaggatga
     3481 tgccgaagtg gatttggtca tcgacaaacc ttcgcaccca cccatcttga tcgaaatcaa
     3541 atcaaagacg atgatccagg aaaaagacat atcttcgctt cagcgcctgg cacgcgatat
     3601 caaaggatca cgcgcatact gtctttcaca ggatccatca ccgcaaatca taggcacagt
     3661 ctcctgcctc ccctgggaaa aggggatatg cgaaatactg gaattgccat cgtcgtaaga
     3721 gagacagctt ccatttctta agggctcctc atatggatga cggtggggtt tggaaaataa
     3781 aaaaaccgcc acaaagcctc tcagacgcga ccttcagggg cggactgaaa cttgttggcg
     3841 actggca
//
LOCUS       DATDUN010000806         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_82901, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000806 DATDUN010000000
VERSION     DATDUN010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82901"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1050
                     /locus_tag="VJC18_11230"
     CDS             43..1050
                     /locus_tag="VJC18_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HLD45997.1"
                     /translation="MKQLKGKHFVTTEDWSKDELDHLLALAHDFKARKKKGEVSTLLQ
                     NQTIFMLFFEQSTRTRNSMSCGITQFGGSSQDLTPDKVQISHGETAKDTAIVLGRMGH
                     AISCRCCFYDKGNKYISELAKWSPIPVMNLQCDLYHPMQMMADTMVMQEKFGQNLKGL
                     NVTISWAYAESHAKPISVPLEQMLMFTRYGMNVTIAHPKEFPLLPAMVDKAHGYAKQA
                     GGSLKFTDNMDDGFKGAQIVIPKNWGGFANFDKWTDSPEQWAEMKANLAKHRDWICDE
                     RRMGLADKNVKYMHALPADRGKEVVDSVIDNEKWSIIYDEAENRLHTAKAVMAATMAG
                     M"
     gene            1136..2080
                     /gene="arcC"
                     /locus_tag="VJC18_11235"
     CDS             1136..2080
                     /gene="arcC"
                     /locus_tag="VJC18_11235"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HLD45998.1"
                     /translation="MNKVVVIAVGGNSLIADDKHQTVPDQCAQAAKTMHHIAGLIEKG
                     YEVVITHGNGPQVGFILLRSEFARERLHTVPLDSCGADTQGAIGYNFQMTMGNELRRR
                     GIDKQVATVVTQVVVDQNDPAFQKPSKPIGPFYKKEEALDRAKTDGWDVMEDAGRGWR
                     RVVASPMPIEIVELKVIKNLIENGVIVVAVGGGGIPVVRDEKGDLRGTAAVIDKDHAS
                     ALLAGELKADLFIISTSVDKVFVNYGKPDQQPIHKMTVTQAREYIAQKQFHPGSMLPK
                     VEAAISFLEKGGKEVIITNFENLEHAISGKTGTRVVVG"
BASE COUNT          556 a          513 c          579 g          467 t
ORIGIN      
        1 cagctttcac ctttcagctt tgagctgatt taaggagtca atatgaaaca gctcaaaggc
       61 aaacattttg taaccaccga agactggtca aaggatgaac tcgatcatct gctggctctg
      121 gcacacgatt tcaaggcccg gaagaaaaaa ggcgaggtga gcaccctgtt gcagaaccag
      181 accatcttca tgctgttttt tgaacagagc acgcgcacgc gcaattccat gagttgcggg
      241 atcacccaat tcggtggcag ttcgcaggac ctcacacccg ataaggtgca aatctcccac
      301 ggcgaaacag ccaaggacac cgccattgtg ctgggccgca tgggtcacgc catttcctgt
      361 cgttgctgtt tttatgataa gggcaacaaa tacatttcgg aactggccaa gtggtccccc
      421 atccctgtca tgaacctgca atgcgatttg tatcacccca tgcagatgat ggctgacacc
      481 atggtcatgc aggaaaagtt tggtcagaat ctcaaagggc ttaatgtgac catcagttgg
      541 gcctatgccg aatctcatgc caaacccatt tcagtgcctc tggagcaaat gctcatgttc
      601 acacgttatg gcatgaatgt gacgatcgct catcccaaag agttcccctt attgcccgcg
      661 atggtcgaca aggcccacgg ttatgccaag caggcgggcg gcagtctcaa gtttaccgac
      721 aacatggatg acggcttcaa aggcgcgcag attgtgatcc ccaagaactg gggcgggttc
      781 gccaactttg acaaatggac tgatagtccc gaacagtggg ccgaaatgaa ggccaatctg
      841 gctaaacaca gggattggat ctgtgatgag cgccgcatgg gtctggccga caaaaacgtc
      901 aaatacatgc acgcactgcc cgctgatcgt ggcaaggaag tggtggacag cgtgatcgat
      961 aacgaaaaat ggtccatcat ttacgatgaa gccgaaaatc gcctgcacac agcaaaagct
     1021 gtgatggccg cgacgatggc aggaatgtga ttcgtggttc gtggttcgtg gttcgtataa
     1081 atgcattttt cgattcacga tccacgattt acgatccacc ggtcatgatt tgtttatgaa
     1141 taaagttgtc gtcatcgccg ttggtggcaa ttcactcatt gccgatgata aacaccagac
     1201 cgtacccgat cagtgtgcgc aagcggccaa gaccatgcat catattgccg ggctcatcga
     1261 aaagggctac gaggttgtga tcacgcacgg caatggcccg caggtcgggt ttattctgtt
     1321 gcgctccgag tttgctcgtg agcgtctgca cacagtcccc ctggattcct gtggcgccga
     1381 cacacaaggc gccattggtt ataattttca gatgaccatg ggcaatgaac tcagacgcag
     1441 ggggatcgat aaacaagtgg cgaccgtggt cacacaggtc gtggtggacc aaaacgaccc
     1501 tgcttttcag aaaccctcca agcccattgg ccctttttac aaaaaagaag aggccttgga
     1561 tcgcgcgaaa acagatggtt gggatgtgat ggaagatgcg gggagaggct ggcggcgagt
     1621 ggtcgcatct cccatgccca ttgagatcgt ggaactcaag gtcatcaaga atcttattga
     1681 gaatggagtg atcgttgtgg ctgttggagg tggcgggatt cctgtcgtgc gcgatgaaaa
     1741 gggtgatttg aggggcacag cggctgtgat tgacaaagat cacgcgtcgg ctttgcttgc
     1801 gggtgaactc aaggcagacc tgttcatcat ctcgacgagt gtcgacaagg tttttgtgaa
     1861 ttacggcaaa cccgatcaac aacctattca caaaatgaca gtgacgcagg cgagagagta
     1921 cattgcccag aaacaatttc accccggttc catgttgccc aaagtcgagg cggccatcag
     1981 ttttttggaa aagggcggca aggaagtcat catcaccaat tttgaaaatc tcgaacacgc
     2041 gatcagcggc aagacaggga cgagggtggt ggtggggtag ggagcacgaa aacacgaaga
     2101 tacgaaaaca cgagg
//
LOCUS       DATDUN010000807         1065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_243058, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000807 DATDUN010000000
VERSION     DATDUN010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243058"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VJC18_11240"
     CDS             complement(<1..1032)
                     /locus_tag="VJC18_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLD45999.1"
                     /translation="MKIKVRINDINRELITEPSENVRSLLRRANISLMRDGCDGQGSC
                     GACTIILDGKTVNSCLLLSPQIDGKTIYTVEHLAKNRQLSAIQTAFLDAGVVQCGYCT
                     PAMLLATKQLLEKTPQPTREQIKSAFSGIFCRCTGYEQYFAAVDLASRRLKDPESLAA
                     KGPEFRDHLRVVGKAKRKVDGPRLVRGDRAFVEDMVQPGTCYLKMLRSPHAHAYIKDI
                     DTSEAEALPGVVHIVTHKNCPDIYYNQAGQGFPEPSPYDRKLISEKVRHVGDRVAAVV
                     AETKELAEQASKKIKVTYEVLQPVFTIDEAARKDAPLIHNSQLEYVVGAPVGLDNSQA
                     NPRDGKIIYQ"
BASE COUNT          228 a          285 c          280 g          272 t
ORIGIN      
        1 ctgatagatg attttgccat cacggggatt agcctggcta ttatcaagtc ctaccggggc
       61 acccacgacg tattccaact ggctgttatg aatgaggggt gcgtccttgc gggcggcctc
      121 gtcaatcgtg aacaccggtt ggagaacctc gtaggtcacc ttgatttttt tgctcgcttg
      181 ctcggcaagc tccttggttt cggccaccac cgcggccacg cggtcaccta cgtgccgcac
      241 tttttcgctg atgagcttgc ggtcataggg tgatggttcg ggaaaccctt ggccagcctg
      301 attataatat atatcgggac agtttttgtg cgtcacaatg tggaccacac ccggcagggc
      361 ctctgcctca ctggtatcga tgtctttgat gtaggcatgg gcgtgcgggg aacgaagcat
      421 ttttaaataa caggtccccg gttgcaccat gtcttccaca aacgccctgt ctccgcgcac
      481 gagtctggga ccatcgacct ttcgcttggc cttgcccacc acacggaggt gatcccgaaa
      541 ctcgggaccc tttgcggcca gagactccgg atctttgagc cgacgactgg ccagatccac
      601 ggccgcaaaa tattgttcat aaccggtgca acgacaaaag attccagaaa aggcagactt
      661 gatttgctct cgggtgggct gtggcgtttt ttcgagcaac tgttttgtgg ccaacaacat
      721 cgccggtgtg cagtaaccgc actgcaccac acccgcatcc aggaaagctg tttgaatggc
      781 agacagttgg cggtttttcg caagatgttc caccgtgtaa attgttttgc catcgatctg
      841 cggtgagagc aacagacagg aattgactgt cttgccatca agaatgatgg tgcacgcccc
      901 acagctcccc tgcccgtcgc acccatcgcg catgagcgaa atgttggcac gtctgagtag
      961 agagcgtaca ttttctgagg gttcagtaat caattcccta ttgatatcgt tgattcgaac
     1021 tttgattttc ataagaatcc taaatcgctt gtgcccttta gggga
//
LOCUS       DATDUN010000808         1469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_575558, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000808 DATDUN010000000
VERSION     DATDUN010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575558"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..173
                     /gene="ffs"
                     /locus_tag="VJC18_11245"
     ncRNA           76..173
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VJC18_11245"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(245..1468)
                     /gene="hutI"
                     /locus_tag="VJC18_11250"
     CDS             complement(245..1468)
                     /gene="hutI"
                     /locus_tag="VJC18_11250"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HLD46000.1"
                     /translation="MIGPFAQVLTMQGLPLTGHIPDERLDVHINAGVLIDGERIISVD
                     KWDELVNLNDVEKFVLSEPCVLLPGFIDVHTHICHAGSRARDYAMRMAGATYLEIANL
                     GGGILDTVRHTREATQEELSRLLWQKCDRQLRHGVTTCEVKSGYGLTVVDELKQLQAI
                     KDIHQTHEIDLVATCLAAHTLPPEFKEHKTYLDFVTRELLPKIKCDRLAGRVDIFVEK
                     SAFTPDLARNYLMTAKAMGFAVVVHADQFTKGGSQVACEVGALSADHLEVSGDAELVA
                     LTGHNVVAVVLPGSTLGLGLPFAPARKMLDHGLCVVLSSDYNPGSAPMGNLLAQVSLL
                     GVSQKLSTAEVLAGITCRAAHALRLDDRGVLAEGLLADMVAFACDDYREILYQQGGLL
                     PKQVWKRGVCVRHKA"
BASE COUNT          342 a          454 c          341 g          332 t
ORIGIN      
        1 ctagattcag gttctagcga gagcaatctc gtgctggttc aaatccagtc ccgggcacat
       61 cgtgatccac ttgggttcac ggcactgcgc aagatcaatt ttatgaatcc cgccaggacc
      121 ggaaggtagc aacggtagta aatgcagtct gtgccgcggt aagccgtgag ccctttgagt
      181 cacgaacctc ttccctttcg cctttcgcct ttcgcctttc gcctttcgcc ttatgccttt
      241 cgccttacgc cttatgcctt acacaaactc ccctcttcca cacctgtttg ggcaacagcc
      301 ctccctgttg atacaaaatc tcgcggtagt catcacatgc aaacgctacc atatcggcca
      361 agaggccttc ggccagcaca ccgcgatcat caagcctcag agcatgagcc gcgcgacacg
      421 taatgcccgc gaggacctct gctgtgctga gtttttgcga cacgcccaag agcgaaacct
      481 gggccaaaag attacccatg ggggccgatc cgggattgta atcgctggag agcaccacac
      541 acaatccgtg atcgagcatt tttcttgccg gggcaaaggg gaggcccaag cccaatgtgc
      601 tccccggcag aactacagcc accacattgt gccctgttaa cgccaccaat tctgcatctc
      661 ccgaaacctc aagatggtcg gcgctcaggg cgcccacctc acaagcgacc tgcgatcccc
      721 cttttgtgaa ctggtccgca tgcaccacca cagcgaaccc catcgccttg gctgtcatca
      781 gataatttcg cgccaggtct ggcgtgaacg cgcttttctc cacaaaaata tccacgcgcc
      841 ccgccaaccg atcacatttg attttgggca acagttcacg ggtcacaaaa tccaaatagg
      901 ttttgtgctc tttaaattct ggtggcaatg tgtgcgcggc caaacaagtg gccacgagat
      961 cgatctcatg cgtttgatga atatctttga tcgcctgaag ctgtttgagc tcatcgacca
     1021 cagtgaggcc atacccgctc ttaacctcgc aggtggtcac cccatggcgc aactgcctat
     1081 cacatttttg ccaaagcaga cgcgacaatt cttcctgggt agcctctctg gtgtgccgta
     1141 cggtgtcgag aatgccgcca cccaaattgg caatttcgag gtaggtggca ccagccattc
     1201 tcatggcgta gtcacgagcc cgtgaaccgg catgacaaat atgcgtgtgc acatcgatga
     1261 acccaggcag gagcacgcag ggttcactca gaacaaattt ttccacgtca ttcaagttga
     1321 ccaactcatc ccatttgtcc acactgatga tccgctcccc atcaatgaga acacccgcgt
     1381 ttatgtgaac atccaatcgc tcatcgggaa tgtgtcccgt aaggggaagt ccctgcatgg
     1441 tcaacacctg agcgaacggc ccaatcaat
//
LOCUS       DATDUN010000809         4536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_326678, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000809 DATDUN010000000
VERSION     DATDUN010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326678"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2035)
                     /locus_tag="VJC18_11255"
     CDS             complement(<1..2035)
                     /locus_tag="VJC18_11255"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HLD46001.1"
                     /translation="MKRWHKILLIFIATLILGVFVYLGWTWFCNEKNQLTDDLNRRAR
                     IIAKSLSRTFIKTIKKDASAHDIEVLESISDQGRTLGYFLCDTNGKLVLQTLLFEGIP
                     YCDIVKDEQTATDPEVENIFYENPAGTLVHIYSAPLFDKNQIFLGTAGIVHDASYIHE
                     RVMAALLWMALSMAMIALLISAATYLASRLIFQRSVNDFISWVKEGHATPRSIANTLS
                     LLKPVSREFTKLNAKLRSAQQTAQEVSQSKTDEEIWTPVRLKAHAMSQIGDKKLIVVS
                     NREPYIHIKEKGIVRVMHPASGLVTALDPVLKATSGLWVAHGSGNADLDVVDEENKVW
                     VPEETPSYQLKRVWLTKQEEEGYYYNFSNEALWPICHPTHERPVFDDQAWEMYNQVNH
                     KFCESVAEEFAKKERPHVLIQDYHFSLLPQMIRNKRADAVTGLFWHIPWPILETFQIC
                     PWKRELLKGMLGANILGFHLPSYCNNFLNTVNALLPVRIDWDRQAIIHEGGTTYVKAF
                     PISIQPWTERNLPDEATIQADMNNWRKQLGIEGTRLVVSVDRLDYTKGFSERMDAIKR
                     FFENNPNFCGQITFVHLAAPSRTHIPRYREFDARIEKKVDDVNWTFGTEDWKPILFLK
                     ENHPPYKVYLFLRLAEVCLVSSLADGMNLVAKEFVAARENNDGVLILSEFAGAV"
     gene            complement(1947..2375)
                     /locus_tag="VJC18_11260"
     CDS             complement(1947..2375)
                     /locus_tag="VJC18_11260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLD46002.1"
                     /translation="MVQGVDVERNGNSVKRSTLIARMGSMGRSTGDHVHYEVFFADEP
                     VDPTMFVESISYKIAIKCFLCDKRNTFPMIRVRILFRRILMVGPPSIRHCVVDGGLFF
                     VQKLPRINGGYYEKMAQNTPDIHRNLDPGCFCLSWLDLVL"
     gene            2528..2935
                     /locus_tag="VJC18_11265"
     CDS             2528..2935
                     /locus_tag="VJC18_11265"
                     /inference="COORDINATES: protein motif:HMM:NF023145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tudor-knot domain-containing protein"
                     /protein_id="HLD46003.1"
                     /translation="MKLNKYILSMVVLFVLMLGVQALGFAAEAYTVGQKIQVEWKGSW
                     YLSTIKEVKDGKYKIHYDGWSDSWDEWVGTNRMKASGASDDFSVGNKVQVKWKGAWYP
                     STIKEVKDGKYKIHYDGWSDSWDEWVGTSRIRK"
     gene            3116..3913
                     /locus_tag="VJC18_11270"
                     /pseudo
     CDS             3116..3913
                     /locus_tag="VJC18_11270"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            3933..>4536
                     /locus_tag="VJC18_11275"
     CDS             3933..>4536
                     /locus_tag="VJC18_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLD46004.1"
                     /translation="MSNRTPEHSHRIPITVWFLGFVSLLMDTSSEIIHSLLPVFMVTV
                     LGVGATTIGAIEGVAEATALITKVFSGVVSDYVGKRKWLAVMGYGLAAVTKPFFALAS
                     SASWIFGARFIDRIGKGIRGAPRDALVADVTPPTIRGAAFGLRQSLDTVGAFLGPLLA
                     MGLMLLWGNNIRSVFWVAVIPAFLAIALLVFGVREPTLTTP"
BASE COUNT         1098 a         1069 c         1140 g         1229 t
ORIGIN      
        1 aaacggcacc ggcaaattct gaaagaatca gcacgccgtc attgttttct cgcgccgcca
       61 caaattcctt ggccaccagg ttcatgccgt cagcaagaga actgaccaga cacacttcag
      121 ccagacgcaa gaacaaataa accttgtagg ggggatggtt ctctttcaga aacagaattg
      181 gtttccagtc ttcggttcca aacgtccagt tgacgtcgtc cacctttttt tcaatgcggg
      241 cgtcaaactc ccggtaacgt ggaatgtgtg ttcgcgacgg agccgccaga tgaacaaagg
      301 tgatctgacc acaaaaattt gggttgtttt caaaaaaacg tttgatggca tccattcgct
      361 ccgaaaaccc tttggtgtag tccaaccggt ccacgctaac gaccaaccgc gtgccttcta
      421 ttcccaattg tttacgccag ttgttcatgt cggcttgaat cgttgcttcg tctggaagat
      481 ttcgttctgt ccaaggctga atactgatgg gaaaggcctt tacgtaagtt gtgccgccct
      541 cgtggataat ggcttgccga tcccaatcaa tccgaacggg caaaagggca ttcacagtat
      601 tcaaaaaatt gttgcagtac gacgggagat gaaaacccag aatgttggct cctaacatgc
      661 ctttcaggag ctctctcttc cagggacaaa tctgaaaagt ttccagaatg ggccacggga
      721 tatgccaaaa cagcccggtc acagcgtccg cacgcttatt tcgaatcatc tgaggcagaa
      781 gggaaaaatg atagtcttgt attaaaacat gaggacgttc tttttttgca aattcttcgg
      841 cgacactttc acagaactta tgattgacct gattgtacat ttcccaggcc tggtcatcaa
      901 aaacgggacg ctcatgcgta ggatgacaga ttggccacaa ggcctcgttg gaaaaattgt
      961 aataatagcc ttcttcttcc tgttttgtga gccaaacccg cttgagctgg taactgggtg
     1021 tttcttctgg aacccagact ttgttttcct catccaccac atccaggtca gcattgcctg
     1081 acccgtgcgc aacccaaagt ccgctagtcg ccttgagcac ggggtcaaga gccgtgacca
     1141 aaccactggc tggatgcatg acacgtacaa ttcctttttc tttgatatga atgtaaggtt
     1201 cacggtttga aacaacgatc agttttttat caccgatctg tgacatggcg tgtgccttga
     1261 gcctcacggg tgtccagatt tcttcgtctg ttttggattg agagacttct tgagccgttt
     1321 gctgggccga gcgtaacttg gcattgagtt ttgtaaattc gcggctcacg ggtttgagaa
     1381 gcgacaacgt gttcgcaatg gaacgcggcg ttgcgtgccc ctccttgacc cacgaaataa
     1441 aatcgttcac tgatctctga aaaatgagcc gcgatgccag gtaggtggcc gccgagatca
     1501 acaaggcaat catcgccatg gaaagggcca tccataaaag agccgccatc acgcgttcgt
     1561 gaatgtacga ggcatcgtgt acgatccctg ccgtgcccaa aaagatctgg tttttgtcaa
     1621 aaagtggggc tgaataaatg tgaaccaacg tcccagcggg attttcgtaa aaaatatttt
     1681 cgacttcggg gtcggttgct gtctgttcat ccttgacgat atcacagtag ggaattccct
     1741 caaacagaag tgtttgtaac acaagttttc cattcgtatc gcaaaggaaa taccccagcg
     1801 ttctcccttg gtcggaaata ctttccaaaa cctcaatgtc atgagcactg gcatcttttt
     1861 ttatggtctt gataaatgtt ctgctaaggc ttttggcaat tatgcgggcg cgtctattga
     1921 gatcatcagt cagctggttc ttttcattac aaaaccaagt ccagccaaga taaacaaaaa
     1981 cacccaggat caaggttgcg atgaatatca ggagtatttt gtgccatctt ttcataataa
     2041 cccccgttga ttcgcggtag cttttggaca aaaaaaagac ccccatccac gacgcaatgg
     2101 cgtatggatg gcggcccgac catcaaaatc ctccggaata aaatccggac cctaatcatt
     2161 ggaaatgtat ttctcttatc acacaaaaaa cattttatgg caattttgta agaaattgat
     2221 tccacaaaca tcgtcggatc aaccggctca tccgcaaaga aaacctcgta gtgcacatgg
     2281 tcgcctgtgg agcgccccat tgaccccatg cgggcaatca gcgtgctgcg ttttacgctg
     2341 ttgccgtttc tttcaacgtc aacgccttgt accaatactt ggtcagacct aaattgaatt
     2401 taaatgattg taattagtta gaagccccga ctacggtgtc ctgcttgagg ggcaaatctt
     2461 gttgaattat ttatagcttt catttagtcc ccaatggtca tttttattat tgatgaaagg
     2521 ggttcacatg aaattgaata aatatatatt atcgatggtt gtgttgtttg ttttgatgtt
     2581 gggtgttcag gctcttggct tcgctgctga ggcctacaca gtagggcaga agattcaggt
     2641 tgagtggaag gggtcatggt atctatctac gatcaaagag gtcaaggatg gcaaatacaa
     2701 gattcattac gacggctggt ccgatagctg ggacgagtgg gtggggacca atcgcatgaa
     2761 ggcctcaggc gccagcgatg atttttcagt tggcaacaaa gttcaggtca agtggaaggg
     2821 ggcgtggtat ccttctacca tcaaagaggt caaggacggc aaatacaaga ttcattatga
     2881 tggctggtcc gatagctggg acgagtgggt ggggacttcc cgtattcgca aataacatca
     2941 attttttttg ggggcgaaag cccccttttt tttcttcgag tcaggcaaag cctggctagt
     3001 ctggtgccgg tttaggttgc tatatgattc aaaaaattac agacaatctt attgagaagg
     3061 ctatgcgtct tgcggtgacg gcacacagcg gtcaggcgcg caagggcgat ggtcaaacga
     3121 aacacttttg gacgaacacc gcgttttgca ggtgctcgaa acagccgagg tgcctgtggt
     3181 gcgtccgctg accctgcgtc actcacaggg ttatgaaacg ttggcgtttg catatccgta
     3241 cccctattgt ctttacccca aatttggggg gcacgcggaa cccgatttgt ctgatgacca
     3301 cagacaatgg ttgggcaggg cgctggcgcg tttgcataat gtcaccagca ctcttgagat
     3361 caggcatcgt ctgatgctca acgcacagac ctatggggac gatcaggtgt cctccatttt
     3421 tgagagacct tttctgccgc gtgacctcag ggcaaatttg gaagacatca ttttgcgttg
     3481 tctcgatctg attgatcctg tttttgatcg tgattggcgc ccctttgccg ttcacgggga
     3541 ttgtcatttg ggcaatgtgt tgtggaatca ccaagggccc acgctggttg attttgacga
     3601 catggttttg gcccccgcca ttcaggacgt gtggatgtta tttcatgggt ccgaagagga
     3661 acaaaccaaa caaaaaaaag ctttttttga aggctatgaa ctgttccgtg catttgatca
     3721 cacctctttt gccttggtgg aaccgctcag aagtctgcgc atgatccggc acacagcctg
     3781 gattggcgag cgatttgacg aagaaatttt taggagaacc ttcccttatt acacagatcg
     3841 caaatactgg gaagaattcc tgctttccat gaaagaacaa tgggcggcgc tcagccatga
     3901 taaagatggg tgacccctgc tgcccctgtt gtatgtcaaa tagaacacct gaacattcgc
     3961 atcgtattcc catcactgtt tggtttcttg gttttgtgag tctgctcatg gatacctcgt
     4021 cagaaataat ccacagcctg ctgccagtgt tcatggtgac cgttctaggc gtgggtgcca
     4081 ccacgatcgg tgccattgag ggagtcgctg aggccacagc tctcatcacc aaggtttttt
     4141 ctggagttgt cagcgactat gtgggcaaac gcaagtggct tgccgtgatg ggttatggtt
     4201 tggcggcggt gaccaagccg ttttttgcgt tggcgagtag tgcaagttgg atttttggcg
     4261 cacgcttcat cgaccgtatt ggcaaaggca ttcgcggtgc tccgcgtgat gccttggtgg
     4321 ctgacgtgac gccaccaaca atccgcggtg ccgcgtttgg actgcgacaa tcactcgaca
     4381 ccgttggtgc cttccttggg cctttgctgg cgatgggact catgctgttg tgggggaaca
     4441 acatccgttc tgtgttttgg gtggctgtga tccccgcctt tttggccatc gcgttgcttg
     4501 tgttcggtgt gcgcgaaccg accttgacaa caccag
//
LOCUS       DATDUN010000810         2512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_205286, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000810 DATDUN010000000
VERSION     DATDUN010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205286"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VJC18_11280"
     CDS             <1..469
                     /locus_tag="VJC18_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46005.1"
                     /translation="KKAICSEAVAIAGFVLSVSKTHKLQLVIDLHEDKYCDKAYVYSQ
                     GRLGARDRVARKIVSIINQHDMPLMQCGATCFAEMISNGIIEKVRDGSLDELLASDQI
                     IVNGNIERGPGAKTVVVVETSVESFSLTQRIACHSDIILSVAQLYDLSLRAET"
     gene            466..1254
                     /locus_tag="VJC18_11285"
     CDS             466..1254
                     /locus_tag="VJC18_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLD46006.1"
                     /translation="MKNYTLLCDESEKQRLRMQATQLYDGPHFLDPFLKKGIKVMDVG
                     CGTGDIARYVANKIKTGQVVAVDRDKDKIDTNVINNGGTKMAHIDYCVGDVYHLAYPD
                     NSFDLTSTRFLLMHLKYPQRAISEMMRVTKPGGFVVAHEGIHNAIWFNPALKHFKIIL
                     NAWKCLMKKKGQDHSIGLSLHHVFMRAGLRQPRMQILTHYAQYGDILFDKYIANWKQH
                     IPSFRKKLANKVSNNNFIILENELDALGSDSSLLELTSVAWGRK"
     gene            complement(1384..1920)
                     /locus_tag="VJC18_11290"
     CDS             complement(1384..1920)
                     /locus_tag="VJC18_11290"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLD46007.1"
                     /translation="MVKRKIINQFLNAFGKRGDNQIIGIVIHCISAIDVAPKKPYDLQ
                     LILGVLNHHNVSADYLIDRKGQIYSLIPSDCYAYHAGISIMPTGETTGMNNNQSTVNK
                     ITVGIELVGHVQKRYTQKQYDSCAWLCLKLICENPLIDLNHIVGHEHVAPGRKKDPGP
                     LFDWNLLFEKIFSFKKKP"
     gene            2035..>2512
                     /locus_tag="VJC18_11295"
     CDS             2035..>2512
                     /locus_tag="VJC18_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46008.1"
                     /translation="MKYQHIIFFVFITLLSASSAFAASPCSVELGELPVLAEGRVMPL
                     EVRASQTLKFMTGKATPLKEDPVITYCRLSFSQQNPDDAILKELFLKTGHVQVREILG
                     LNKGVSTISPDQVLEKLQGIQNHVYQLEQNGGNRVVIDELKALLAKTQLFYELSHGG"
BASE COUNT          719 a          511 c          594 g          688 t
ORIGIN      
        1 aaagaaagcg atttgttctg aagccgtggc aatagctggt tttgttttga gtgtttcaaa
       61 aacacacaag ctccaactgg tcattgattt gcacgaagac aaatactgcg ataaagcata
      121 tgtctactca caagggcggc tgggtgcgag agatcgcgtt gccagaaaaa ttgtttccat
      181 cattaatcag cacgacatgc ccttgatgca atgtggtgca acatgttttg ctgaaatgat
      241 ctcaaatggg attatcgaga aagttcgaga tggatcgctt gatgaactgc tggcctctga
      301 tcaaatcatt gttaacggca atattgaacg agggcctggc gcaaagacag tcgttgtggt
      361 tgagacgtct gtagagagtt tctctttgac tcagcggatc gcttgtcact cggacatcat
      421 tttgtctgtt gctcaattgt atgatttgtc tttgagggcc gaaacatgaa aaattatact
      481 ttgttgtgtg atgagtcaga aaaacaaagg ctcagaatgc aggcgacaca gttgtatgat
      541 ggacctcatt ttttggaccc gtttttaaaa aagggcataa aagttatgga tgttggttgc
      601 ggcacgggtg acatcgcccg ttatgtggcc aataaaatca aaactggtca agtcgttgct
      661 gttgataggg acaaggacaa aattgacacc aacgtcatca acaacggcgg tacaaaaatg
      721 gcacacatcg attattgcgt tggggacgtg tatcacctag cttatccgga caacagcttt
      781 gatcttacct ctacacgttt tttgttgatg cacctgaaat atccccaaag agccatttca
      841 gaaatgatgc gcgtgaccaa gcctggtggt ttcgtggtcg cacacgaggg aattcacaat
      901 gctatctggt ttaatcccgc actgaaacat tttaaaatca tattgaacgc ctggaaatgt
      961 ttgatgaaaa aaaagggtca ggatcactcc attgggttgt cgttgcacca tgtttttatg
     1021 cgcgcaggct tgagacagcc gagaatgcaa atattgacgc actatgcaca atacggggat
     1081 attctttttg ataaatacat tgccaattgg aaacaacaca taccaagttt cagaaaaaaa
     1141 ctcgcgaaca aagtttcaaa taacaatttc atcatattgg agaacgaact ggatgcattg
     1201 ggttcagaca gttctcttct tgagttgacc agtgtggctt gggggagaaa ataggaccgg
     1261 cattatgtta ttgagaagag taacgatcct gcttgttttc actgggtgaa tggcgagtcc
     1321 cggtgttcac aaagaaggca taaggcaaat gctcaaagga tgagcaatgg gaaacggggg
     1381 gcatcatggc ttttttttga atgaaaatat tttttcaaaa agcaaattcc agtcaaacag
     1441 aggaccggga tcttttttgc gtccaggtgc cacgtgttca tggcccacaa tgtggtttag
     1501 atcaatgagg ggattttcgc atatgagttt gagacacaac catgcgcagg aatcatattg
     1561 tttttgcgta taccgttttt ggacatgccc taccaactca atgcccacgg taattttatt
     1621 aacggtagat tgattgttgt tcatacctgt tgtttcacca gttggcatga tggatatgcc
     1681 cgcatggtag gcataacaat cggaagggat gagggagtag atttgcccct ttctatcaat
     1741 aagataatcc gcagatacgt tatggtggtt taacacacca agaataagct gaagatcgta
     1801 gggctttttt ggagccacgt caatggcact gatgcaatga atgacaatgc caatgatttg
     1861 gttgtcacca cgtttcccaa aagcattcaa aaattgattg ataatcttgc gtttgaccat
     1921 gtgcctcttt acttatcaaa aaaatggtcg tactccgctg tcagaaatct gttccgcact
     1981 acggacatgg gataactcaa tttgggcacg taaaacaatt tgtaaaggga cacaatgaaa
     2041 tatcagcaca tcattttttt tgttttcatc actttactgt ctgcctcatc ggcctttgcc
     2101 gcatcgccct gttctgtgga actgggcgaa ttgcctgtgt tggcggaggg cagggtcatg
     2161 cccctcgaag tgcgcgccag ccagacactc aaattcatga caggcaaagc aacccccctc
     2221 aaagaagatc ctgtcatcac ctattgccga ctgagttttt cacaacagaa tccggatgat
     2281 gctattttga aagaactgtt cctcaaaaca ggtcatgtgc aggtcaggga aatattgggg
     2341 ctcaacaagg gcgtctccac gatcagtccc gaccaagtgc tcgaaaaact gcaaggcatt
     2401 caaaatcatg tttatcaact ggaacaaaac ggcggcaatc gcgtggtgat tgacgagctg
     2461 aaagccctgt tggcaaaaac acagttgttc tatgaactgt cccacggtgg cg
//
LOCUS       DATDUN010000811         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_618421, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000811 DATDUN010000000
VERSION     DATDUN010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618421"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VJC18_11300"
     CDS             complement(<1..1060)
                     /locus_tag="VJC18_11300"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HLD46009.1"
                     /translation="MNKYSCFFIAVLLSLSCLLLGACEKGEESAVVVGNTKISEAGNN
                     VVKEKTDISEKEKATDPKYSWDLSEIYPDVAAWRQAKDTLQKKLPDIARYQGRLAESS
                     STLVGALDLMFGLKKEGYRLSGYAMFLSDLDTRVAANEALVQEVDTLDKQFSELSAFV
                     VPEILSLDQSVIDGFVATDPKLKVYQFYLANLLRMKAHTRSPEVEKLVAQAGQALDAP
                     EKAYGTFSDAELPAEEIELSTGEKIRLDKSGYVKYRSVQNLTDRHNIFKTFWNTYDKF
                     KATLGTLLYGEVMGHLFLKDVKGYQTSLEAALDKDAIPASVYEQLIKDVHDNLPVFHR
                     YLKLRQKMMGLSELKYEDL"
     gene            1184..2056
                     /locus_tag="VJC18_11305"
     CDS             1184..2056
                     /locus_tag="VJC18_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46010.1"
                     /translation="MADPISYNMATTKLMSQLTLLDPTPEDLAFSDADIERLGWEERV
                     ALYHAFAQLSQSNEWVPERNIACRLFSTPTQSQIAVDLDEQLLTSPRAQEYEQNFTTA
                     IMLMQAVASTGNADYKSLIEKSCWPATPQGDIAMILVDGLESFLRRNGAPVPDLTAKD
                     ALSVRIATSSDPAVQKREYSLFIFVDAQKTQGDFYKIFAALVHEYIGHAFLYMTAHPN
                     VTTAPTIAQQGRQSQESSVAEEIAAFKRSVRFLEMAVSFCRTDASFAFAAPKIEKLLE
                     EEKFRLRSYLDAAK"
     gene            complement(2073..>2431)
                     /locus_tag="VJC18_11310"
     CDS             complement(2073..>2431)
                     /locus_tag="VJC18_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46011.1"
                     /translation="VVVKLEEAGVTDLLQTLFLIHSFDLVNKLDTVTQAVAFYQQVVK
                     GECKTVLDLLDRLHQAPTRIQMGMGPFVAQTFRVMLPKRLSTGQKVELWGKFFAYFDR
                     CHMPEVALECARAQQG"
BASE COUNT          615 a          657 c          566 g          593 t
ORIGIN      
        1 agagatcttc gtatttgagt tcggagagcc ccatcatttt ctggcgcagt ttaagatagc
       61 gatgaaatac aggcaggttg tcgtgcacat ccttgatgag ctgttcataa acagatgcgg
      121 gaatggcatc cttgtccaat gcggcttcca gactggtttg ataaccttta acgtctttga
      181 gaaaaaggtg ccccatcacc tcgccataaa gcagagttcc cagcgtggcc ttgaatttat
      241 cgtaagtgtt ccaaaaggtt ttaaagatat tgtgacgatc cgtcaggttt tgcacggaac
      301 ggtatttgac ataaccggat ttatccaacc ggatcttttc tccggtggac aactcaattt
      361 cctctgccgg taactcggca tcggaaaaag ttccataggc cttttccggg gcatccaggg
      421 cctgccctgc ctgcgcgacc agtttttcga cctcggggct tcgggtgtgt gccttcatcc
      481 gcagaaggtt ggccagataa aactggtaga ccttgagctt gggatctgtc gccacaaagc
      541 catcaatgac ggattgatcg agtgacaata tctcgggcac cacaaaggca ctcaactcac
      601 tgaattgttt gtcgagggta tccacctcct gcaccagggc ctcgttggcc gcgacgcgtg
      661 tgtcgagatc gctcaaaaac atggcatacc cgctcaggcg atagccttct tttttgaggc
      721 caaacatgag gtcaagcgca ccgacaagcg tgctggacga ttcagccaag cggccctgat
      781 agcgggcaat gtctggcaat tttttttgca gggtgtcttt ggcctgtcgc cacgcggcca
      841 cgtcgggata aatctcggac agatcccaac tgtatttggg atctgtggct ttttcttttt
      901 cagaaatatc tgttttttct ttgaccacgt tgttccctgc ttcagaaatc ttcgtattcc
      961 ccacgacaac agcactctct tcgccttttt cgcaagcacc aagcagtaaa cacgacagcg
     1021 aaagcagtac agcaatgaaa aaacaagaat atttattcat aacatccccc ctatttggat
     1081 taagagtccc cgttaatgcg ggcaaaatga agtgtcaatg aacagaattt agataaaaaa
     1141 tgtagcaaca accagactcc aataaccgat atagggagtg attatggcag atcctatcag
     1201 ttataacatg gccaccacca aactcatgag ccaactgacc ctgctggacc ccactcctga
     1261 ggaccttgct ttttctgacg cggatataga acgcttgggc tgggaagaac gggtcgcgct
     1321 ataccatgcc tttgcgcaac tgtcccagag caatgaatgg gttcccgagc gcaacattgc
     1381 ctgccgtctt ttttccacac ccacacaaag ccagatcgct gttgacctcg atgaacagct
     1441 cctgacctca ccccgcgcac aggaatatga gcagaatttc accacggcca tcatgctcat
     1501 gcaagccgtt gccagcacag gcaacgccga ttacaaatcc ctgattgaga agtcctgctg
     1561 gccagccacg cctcagggag atattgccat gattctggtc gatggcctag aatcgttttt
     1621 gagaagaaat ggcgcccctg tgcccgattt gacagcgaag gacgcgctga gcgttcgcat
     1681 cgccacctca agtgatcccg cagtacaaaa aagagaatac tccctgttta tatttgtgga
     1741 tgctcaaaaa acacagggtg atttttacaa aatttttgcg gccctggttc atgaatacat
     1801 cggccatgcc tttctctaca tgaccgcgca ccccaacgtc accaccgcac ccacgatcgc
     1861 gcaacagggt cgtcaatcgc aggaatccag tgtggccgag gaaatcgcgg ccttcaagag
     1921 aagtgtgcgt tttttggaaa tggcggtttc tttttgccga accgatgcct cgtttgcctt
     1981 tgccgcaccc aaaattgaaa aacttttgga agaggaaaaa ttccggttgc gatcctatct
     2041 ggacgccgcc aaatagacac cattcctgcg cgtcaaccct gttgcgcgcg agcgcactcc
     2101 agagccacct cgggcatgtg acaccgatca aagtaggcaa agaattttcc ccacagttcc
     2161 actttttgcc cagtggataa tcttttcggt aacatgacac gaaacgtctg ggccacaaaa
     2221 ggccccatgc ccatttgaat cctggtcggc gcttggtgca accggtcgag caaatccagc
     2281 acagtcttac actcgccttt gaccacctgc tggtaaaacg cgacagcctg tgtgacggtg
     2341 tcgagtttat tgaccagatc aaagctgtga atcagaaaca gtgtttgcag caaatcagtc
     2401 acccctgcct cttccaactt gacgacaacc t
//
LOCUS       DATDUN010000812         1044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_243328, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000812 DATDUN010000000
VERSION     DATDUN010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243328"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1044)
                     /locus_tag="VJC18_11315"
     CDS             complement(<1..>1044)
                     /locus_tag="VJC18_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HLD46012.1"
                     /translation="PLETNGVVGFVRDVAGDLVVNATVYFVPATDIPSDAIDLSTIEN
                     ARQSTVDEPLEDTIASKGGNYTKAITDANGIYRVATLPGGAYFITVVPATADKGHLPG
                     GSLCRKVKNSADLIGKQMDIAISATHSEKAEYVGPSACLNCHSGTDHEKKTLHMLGIR
                     VIGKTGPFQDSSRFPTWNDPLAKFTTSGTTLYYFGYNGNTTSPDWKASETNPGAGVSF
                     TAKLYKDGAGKFFVDLTNVAGTAATQTYEVELSYGGGLYKQRYVTKIGDSRYILPIQF
                     NFQGQTDEVSSPSSRWVWQQYNAQNWYDETNKSLKTPAKGKAFDNNCAGCHFTGIKVT
                     GDATAGFKAHGVPD"
BASE COUNT          241 a          263 c          253 g          287 t
ORIGIN      
        1 atcaggcacg ccgtgtgcct taaaaccagc tgtcgcatcg cctgtgacct tgattcccgt
       61 aaagtgacaa cccgcacaat tgttgtcaaa ggctttgccc ttggctggtg tcttgagcga
      121 tttattggtc tcatcgtacc agttttgtgc attgtattgt tgccagaccc accgtgatga
      181 tgggctactc acttcgtcag tttgtccttg gaaattaaat tggatcggca ggatatagcg
      241 actatcgcca attttcgtga cgtatcgttg tttgtaaaga cctccaccat aagagagctc
      301 cacttcatag gtctgcgttg cggctgttcc tgcaacattg gtgaggtcta caaaaaactt
      361 acccgcaccg tctttgtaaa gcttcgccgt aaaactgacc cctgcgccag gattggtttc
      421 agaagccttc cagtcaggtg acgtggtgtt gccattgtat ccaaaatagt agagggtcgt
      481 gcctgaggtg gtgaattttg caagagggtc attccacgtg ggaaaccgag agctatcctg
      541 gaagggacct gtttttccga tcactcgaat ccccaacata tgcagggttt ttttctcatg
      601 atcggttccg ctgtgacaat ttaagcacgc ggagggaccg acatattcag ctttttcact
      661 gtgagtcgcc gaaatagcaa tgtccatttg tttgccgatg agatctgcgc tgtttttcac
      721 ctttcggcag aggcttccac ccggaagatg ccccttatct gctgtggcgg ggacaaccgt
      781 gataaaatag gcaccaccag gcagggtagc cacacggtaa atgccgttcg cgtcggttat
      841 cgctttcgta taatttccac ccttgcttgc gatagtatcc tcgagtggct cgtcaaccgt
      901 gctctgacgc gcattctcga tggttgacag atcaatggca tcacttggaa tatcggtggc
      961 aggaacgaaa tagacggtag cattcaccac taaatcaccg gccacatcac gaacaaaacc
     1021 cactacacca tttgtttcta atgg
//
LOCUS       DATDUN010000813         1002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_243418, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000813 DATDUN010000000
VERSION     DATDUN010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243418"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..>1002)
                     /locus_tag="VJC18_11320"
     CDS             complement(78..>1002)
                     /locus_tag="VJC18_11320"
                     /inference="COORDINATES: protein motif:HMM:NF020610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HLD46013.1"
                     /translation="GALDETSRELKRYIRLCNYSSGLCMPEIAVMGAMEGLDYLLNDA
                     MYGVWFRDINMKRTLIDQFFSRIIIARAGITINTGEDNYLTTSDAFNNHFQVLSSHFL
                     NECFAKHAGLRDDQIGLGHAFEIDPAIEDSFLLEVAMAQLVREIFPRCPIKYMPPTKH
                     KTGDIFFGHVLDTLFNVAGVMTDQGIQLLGMATEANHNPHLQDRYWSLKNANYVFKSA
                     KSLSDEIEYSPNGKIMRQARHVLDRSFRFLKDISSIGLLESIEKGMFAEMPRAKEGGR
                     GYDGVFEKSRRYLNPFAELILPGQNRGGRRE"
BASE COUNT          240 a          264 c          260 g          238 t
ORIGIN      
        1 ccttgattct gggatcaacc gacacgggat gatggttgcg acgacgcccc tctgcgctat
       61 tttgacgata atctttttta ttcacgacga cctccgcgat tctgaccggg caaaatcaac
      121 tctgcaaaag ggttgaggta gcgacgggat ttttcgaaca cgccatcata accccttccc
      181 ccttctttgg cgcggggcat ctcggcaaac atgcccttct cgatcgactc caaaagacca
      241 atactggaaa tgtccttgag gaaccggaac gagcgatcca acacatgccg ggcctgtcgc
      301 atgatctttc cgttgggaga atactcaatt tcatcgctga gggatttggc ggacttgaaa
      361 acatagttgg cgttttttaa cgaccagtaa cgatcctgca aatgcgggtt gtgattggct
      421 tcggtggcca tccccagcaa ctggattccc tgatcagtca tcaccccggc gacattaaaa
      481 agtgtgtcga gcacatgacc aaaaaaaatg tcccccgttt tgtgtttcgt ggggggcatg
      541 tacttgatgg ggcaacgggg aaagatctcg cggaccaatt gtgccatagc cacttccaac
      601 agaaaactgt cctcaatggc tgggtcaatt tcgaaggcgt gccccagccc aatctgatcg
      661 tcgcgaaggc ccgcgtgttt ggcgaaacac tcattgagga aatgactcga caacacctga
      721 aaatgattgt tgaaggcatc ggatgtggtg aggtaattgt cttctcccgt gttgatagtg
      781 attcccgcgc gggcaatgat gatgcgggaa aagaactggt cgatgagtgt gcgcttcatg
      841 ttgatgtcgc ggaaccacac cccgtacatc gcgtcattga gcaggtagtc cagcccttcc
      901 atggccccca tcacggcaat ctcgggcata cacagtcccg acgaataatt acacaggcgg
      961 atgtaccgct tgagttcacg tgaggtttcg tccaaggccc cg
//
LOCUS       DATDUN010000814         4508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_327027, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000814 DATDUN010000000
VERSION     DATDUN010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327027"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="gatB"
                     /locus_tag="VJC18_11325"
     CDS             <1..990
                     /gene="gatB"
                     /locus_tag="VJC18_11325"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLD46014.1"
                     /translation="EIVTEPCIHSPEEAAVFLKALRSLVRYTEVCDGNMEQGSLRCDA
                     NISLAPLANNTLGTKVEIKNLNSIKFIEKALKHEVQRQDALLSKGKAVVQETRLFDDA
                     QGITLTMRSKEEAHDYRYFPDPDLVPLIIDETWINGCRSQLPELATARAQCFVRDFKL
                     SEYDAVLLTGERKLADFFEKVVALYAQPKKVANWIMTELMRELNHSNKDITEIPVTPS
                     HFAGLLSSIDEGIISATVAKKVFEEMFRTGEPPQDIIAKQNLTQIHDTNAIVEAITSV
                     IAAHPEQVKDYQSGKEKVFAFLVGQVMRRLQGQANPQMVNDLLKKVLTPKSAC"
     gene            complement(1018..2220)
                     /locus_tag="VJC18_11330"
     CDS             complement(1018..2220)
                     /locus_tag="VJC18_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLD46015.1"
                     /translation="MKRILCLGLVLMAFCLVLLAPAEVRAWTDKIYKDIATFSKVLDI
                     VDKLYVQEVNEDTAMEGAINGLLQTLDPHSIYMAPEAYKSFSSETKGRFGGIGIEVSI
                     KDGILTVVSPLEGTPAWDAGIKAGDKILTIDGQSTKHMSLNDAVKLMRGPVGKALSLT
                     IWREGKKGAKTVNITREMIKVSAIRTEDLGDGYAMFKIITFQEGVAKQLWTAIEEFVG
                     KKGELKGMILDLRDNPGGLLTEAVRISDFFIAEGVIVSTKGRSQETEVSLAHKKGTLP
                     NFPMIVLINEGSASASEILAGALKDHKRARILGTNSFGKGSVQTLINLENGGAVKITI
                     AHYFTPKDKLIDGKGIPPDIKIDTKGFLKKNKTKFKKDKASQKIERDEFYNYQRDEAL
                     SLLKKTGH"
     gene            complement(2332..3261)
                     /locus_tag="VJC18_11335"
     CDS             complement(2332..3261)
                     /locus_tag="VJC18_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLD46016.1"
                     /translation="MNLATREAYGMKLAQLGETHPEIIVMDADLSGSTKTADFKKKFP
                     QRFFNVGVAEQNLMGTAAGIALSGHTVFVSTFAIFATGRAWEPVRQSIAYPKANVKIC
                     ATHAGITVGEDGASHQSIEDIALMRVIPGMQVLVPADAHQTMSMIDYLVGNNGPAYLR
                     LGRAKTDQVFDHAYQFCLGKGDVLKQGKDICLMTTGFVTKATLDAAQELDQKGISTTV
                     VCFGSIKPIDEQLIVGMARTHKVLFSVEEHSVIGGLGGAIAEVLTAQYPAKLHRLGLQ
                     DEFGQSAPYPVLLRHYKLDKEGIVGSVIERLKA"
     gene            complement(3476..4303)
                     /locus_tag="VJC18_11340"
     CDS             complement(3476..4303)
                     /locus_tag="VJC18_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HLD46017.1"
                     /translation="MNKSIPELQNIARELRVTIVKTIHKAKSGHPGGSLSCIDMLTAL
                     YFYKLNYDSKNPRWEDRDRVVLSSGHTCPALYSILARLGVFPSEELNHLRQLGSPLQG
                     HPKYNLDYGIEMSTGSLGQGMSVANGMALAARAQNKKYRVYSLETDGGSQEGMMWEAT
                     MLAAHYKLSNRCILFDLNNVQIDGFVSDVMGVEPVADKFRAFNWHVIEVDGHNMQQIC
                     SALDEAATVTDKPQAIIGKTIIGKGVSLFENKAKYHGTPPTDEELAVALKELNIAQS"
BASE COUNT         1139 a         1230 c          991 g         1148 t
ORIGIN      
        1 gaaatcgtca cggagccctg tattcactca ccagaagagg ccgctgtttt tttgaaggcc
       61 ctgcgttctc tggtgcgcta cacggaggtc tgcgacggca acatggaaca ggggtcactg
      121 cgctgtgacg ccaacatttc actggcacct ctggctaaca acacactggg cacgaaggtc
      181 gaaatcaaaa acctcaactc tatcaaattc atcgaaaagg ccctcaaaca cgaggtgcaa
      241 agacaagatg ccctgctctc caaaggcaag gccgtggtgc aagaaaccag attgttcgat
      301 gatgcgcagg gcattaccct gaccatgcgg tcgaaagagg aggcccacga ctaccgttat
      361 tttccagacc ccgatctggt tcctctcatc atcgatgaaa cctggatcaa cggctgtcgt
      421 tcgcaactgc cggaactggc gactgctcgc gcacagtgtt ttgtgcgaga ttttaaactg
      481 tccgaatacg atgctgtttt gctcaccggt gagaggaagc tggccgattt ttttgaaaaa
      541 gtcgtagccc tttacgcgca acccaaaaaa gtcgccaact ggatcatgac cgaactcatg
      601 agagaactca atcacagcaa caaagacatt acagaaatac ccgtcacgcc gtcccacttt
      661 gcagggcttt tgtcatccat tgatgaaggg atcatctccg ctaccgtagc caaaaaagtt
      721 tttgaagaga tgttccgtac cggggagcca ccgcaagaca tcattgccaa acaaaatctc
      781 acgcaaattc atgacacgaa cgcgatcgtc gaggccatca cttctgtcat tgcggcacac
      841 ccagagcagg taaaagacta tcaaagcggc aaagagaagg tattcgcctt tttggtagga
      901 caagtcatgc gccgcctgca gggccaagcc aacccgcaaa tggtgaacga tttattgaaa
      961 aaggtgttga ccccaaagtc cgcttgttga atccgatgga tgtgctctgc aggtgattca
     1021 gtgacctgtt tttttcaaca aactcaaggc ctcgtcccgt tgataattat agaattcatc
     1081 tctttcgatc ttctgtgagg ccttgtcctt tttgaatttg gttttgttct ttttgagaaa
     1141 tcctttggtg tcgatcttga tgtctggagg aatacccttg ccatcaatga gtttgtcctt
     1201 gggtgtgaag tagtgggcaa tcgtgatctt gacggcacca ccattttcga gattgatcag
     1261 cgtctgaacg cttcccttgc caaacgaatt ggttcccaga atcctggctc ttttgtgatc
     1321 tttgagtgcc cctgccagaa tttcggacgc gctggcagag ccctcgttga tgaggacaat
     1381 catcggaaaa ttcggcaacg tccctttttt gtgggccaag ctcacctcgg tctcctggct
     1441 gcgccccttg gtgctcacaa tcacgccctc agcaataaaa aaatcactga tgcgcaccgc
     1501 ttctgtcaag agaccacccg gattgtccct caaatccaga atcatccctt tgagttcacc
     1561 ctttttgcca acaaattcct caatcgcagt ccacaactgt ttggccaccc cttcctgaaa
     1621 cgtgatgatc ttgaacatag cgtacccatc acccaaatcc tcagtccgga tggccgacac
     1681 cttgatcatc tcgcgcgtga tgttaacggt cttggcgccc tttttccctt cacgccaaat
     1741 ggtgagactc agcgccttgc cgacaggccc cctcatgagc ttgacggcat cgttgagtga
     1801 catgtgtttt gtggattgcc cgtcaatagt cagaatcttg tccccagcct taatgcccgc
     1861 atcccaagca ggggtgccct caagtggcga cacaacagtc aagatgccat ctttgataga
     1921 cacttcgata ccgatccctc caaaccgtcc tttggtctct gagctgaaac tcttgtacgc
     1981 ctcaggtgcc atgtaaatcg aatggggatc cagggtctgc aacaaaccgt tgatggctcc
     2041 ttccatcgcc gtgtcttcat tgacttcctg aacgtataat ttatcgacga tgtcgagtac
     2101 cttggaaaaa gtggcaatgt ctttgtaaat tttgtctgtc caggcccgca cctcagcagg
     2161 ggcaagcaag accagacaaa atgccatcaa caccaatcct aaacacaata ttcttttcat
     2221 aatttattcc taaaacaatc caactccttc aactgtttca attagcaaca tactattggc
     2281 aattgactgt tgaagcagtt gagaattgaa gcagttgttt tgccttgagc cttacgcctt
     2341 aagcctttct atgaccgacc ccacaatgcc ttctttgtcc aacttataat gcctgagcaa
     2401 aacgggatac ggcgctgact ggccaaattc gtcttgtagc cccagccggt gtaactttgc
     2461 gggatactgg gctgtcagaa cctcggcaat cgcccctccc aacccgccaa tgactgaatg
     2521 ttcctcaaca ctaaataaaa ccttgtgcgt cctggccatg cccacaatca attgttcgtc
     2581 gatgggtttg atcgatccaa aacacaccac agtcgtcgaa atcccttttt gatccagttc
     2641 ctgtgcggcg tccagcgtgg ctttggtcac aaaaccggtg gtcatgagac agatgtcttt
     2701 accctgcttg agcacatcgc ctttgcccaa acaaaactgg taggcatgat caaaaacctg
     2761 atctgttttt gccctgccca aacgcaaata cgctgggcca ttgttcccca ccagataatc
     2821 aatcatactc atggtctgat gggcatcggc aggaaccaaa acctgcatgc cgggaatcac
     2881 gcgcatcaaa gcgatgtctt cgattgactg gtgactcgcg ccatcctcac caaccgtaat
     2941 gcctgcgtgc gtggcacaaa tcttcacgtt ggcttttggg tacgcaatgg attgtctgac
     3001 aggttcccac gcgcgccccg tggcaaagat cgcgaacgtc gacacaaaaa cagtgtgacc
     3061 agacaacgcg atgcctgcgg ctgtgcccat gagattttgc tccgcaaccc ccacattaaa
     3121 aaaacgctgg ggaaacttct ttttaaaatc cgccgttttg gttgaccccg ataaatccgc
     3181 atccatcacg ataatttcgg gatgcgtctc tcccaactgg gccaatttca tgccgtatgc
     3241 ttcgcgtgtt gctaaattca atagttccat gtgttctcca caagctcaaa ggcaaaagct
     3301 caaaggtatg tgaatattga atatcgaaca aggaatgtcg aatatcgaaa tttgtcatcc
     3361 ctgcgaacgc agggatccag aattattgca aacatggatt ccagcattcg ctggaatgac
     3421 aaccacttcg aaattccttg ttccttgttc gtgattcgac attcgtttaa tcctttcagc
     3481 tttgagctat attcaactct ttcaatgcca cagccaactc ctcatccgtc ggtggtgtgc
     3541 cgtgatactt ggccttgttt tcaaataacg acacaccctt gccaataatg gttttgccaa
     3601 tgatggcctg aggcttatcc gtcacagtgg ccgcctcatc gagagcagaa caaatttgtt
     3661 gcatgttgtg gccgtcgact tcgatcacat gccaattgaa ggcccggaat ttatcagcca
     3721 ctggttccac acccatcaca tcagacacaa aaccatcgat ctgcacatta ttgagatcaa
     3781 acaaaataca acgattcgat aatttgtaat gcgcggccaa catggtggcc tcccacatca
     3841 tcccctcctg cgaacccccg tctgtttcca gagaatacac gcggtatttt ttgttttgcg
     3901 ctcgcgccgc cagagccatg ccattggcca cgctcatgcc ctgacccaat gaacctgtgg
     3961 acatctcaat gccataatcc agattgtatt tgggatgccc ctgcaagggc gaacccagtt
     4021 gacgcaaatg gttcaactcc tcagaaggga acacaccaag tcttgccaga atgctgtaca
     4081 atgcggggca ggtgtgtccc gacgacaaca ccacacgatc gcggtcttcc catcggggat
     4141 ttttggaatc gtaattgagc ttgtaaaaat acaatgccgt gagcatgtcg atgcacgaca
     4201 aagagccccc cgggtgtcca ctcttggcct tgtgtatggt tttgacgatg gtgacgcgca
     4261 attctcgcgc gatgttttgc agttctggga tggatttatt cataggtata ttcgtgcatc
     4321 gttaatcgtg gatcgtggat cgtaaatcaa tccatcgaat tgtcatccct gcaaaagcag
     4381 ggatccagag ttacaaaaaa catggactcc agcctttcag ccagaggact gatcccgctc
     4441 tgcgggacgc tggaatgaca aattctttct ttcgatccac aatccacaat ccacgattca
     4501 cgattcac
//
LOCUS       DATDUN010000815         1481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_162196, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000815 DATDUN010000000
VERSION     DATDUN010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162196"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VJC18_11345"
     CDS             complement(<1..604)
                     /locus_tag="VJC18_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HLD46018.1"
                     /translation="MDQFTLETIFSFSDQYFDLLIAIGLFLCLMLFRIHRLQSARNRF
                     SQNTIAPALNKTNFALSLKKLLLGTQKQLDELLPELEEVLLAADVGVGATEKLLASLK
                     EHSFSSKEDIFLFIKERLKKTLSPQDHFSIDPAKKPYVIYLAGVNGSGKTTTIGKLAN
                     QFKQQGHKGMLVAADTFRAAAVDQLRIWAKRNAVAFVGGAV"
     gene            751..>1481
                     /locus_tag="VJC18_11350"
     CDS             751..>1481
                     /locus_tag="VJC18_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46019.1"
                     /translation="MKLNNIILCLVMIGLYGCASTEMGSLEAAKFALDKGEYTEAITE
                     ATEALNANPANYEAARVLASAYFARSGFDYFDIAEGVMDLQNSDTPNFQQVADVFPAT
                     ADLGDLRSAIEVLEGLAGIGAASLEDTEELADAAFDLGLMEIVEHFALGVYGADYFDD
                     NDVTEADVTDITEAYASHVQTDLVDFDNRLIAAGVANDEDWLEEIRQTFCILEPISAG
                     EGFTLAEYQAFVGCQLSSTPDTFDTA"
BASE COUNT          368 a          324 c          403 g          386 t
ORIGIN      
        1 tgacagcccc tcccacgaag gccacagcgt ttcttttggc ccaaatgcgt agttgatcaa
       61 cggcggcggc ccgaaacgta tcagcggcta ccagcatgcc tttgtgtcct tgctgtttaa
      121 actgattggc caatttgcct atggtggttg ttttgccact gccattgacc ccagccagat
      181 agatcacata gggttttttt gcagggtcta tcgaaaaatg atcctggggg gacagggttt
      241 ttttaagacg ttccttaatg aagaggaaga tgtcttcttt ggaggaaaag ctgtgttcct
      301 tgagcgaggc gagtaatttt tcggttgcgc ccacgcccac atcggcggca agcaggactt
      361 cttcgagttc ggggagcagt tcgtccaact gtttttgtgt tcccaacaac agctttttca
      421 gggacagggc aaaatttgtt ttgttgaggg caggggcgat ggtgttttga gagaaccggt
      481 tacgagcaga ttgtaaccgg tggatgcgaa ataacatcaa acataaaaaa agccctatag
      541 cgatcaacag atcgaaatac tgatcggaaa aagaaaaaat agtttctaaa gtgaattgat
      601 ccatgttttg ttcacccgtt gttaaggcgc cctggctatc acaaccacca ataaaataaa
      661 ctcaaaaaaa accactggag ttatttaaaa agttggatta gagttttgca tcatttcgta
      721 ttgattaggt cattaatcta gggggtaaat gtgaaactca ataatattat cttatgtctt
      781 gtcatgatcg gtttgtatgg ttgcgcgtcc accgagatgg gcagtctcga ggccgctaaa
      841 ttcgccctgg ataagggtga gtacacagaa gccattacag aggcaacaga ggcactcaac
      901 gccaacccag ccaattatga ggccgcacgg gttctggcca gcgcctattt tgcccggagt
      961 ggtttcgatt atttcgatat tgctgagggt gtcatggatt tgcaaaacag cgacacacca
     1021 aacttccaac aagttgccga tgtgtttcct gcgacggccg atttggggga cttgaggagt
     1081 gccattgagg tgctcgaggg gctggccggc atcggtgccg cctcactcga agataccgaa
     1141 gaactggccg atgcggcgtt tgatctgggc ctcatggaaa ttgtcgagca tttcgcgttg
     1201 ggagtttacg gtgcggatta tttcgatgac aacgacgtga ctgaggcgga tgtgacagac
     1261 atcactgagg cctatgccag ccatgttcaa acggatctgg tcgattttga caatcggttg
     1321 atcgcggcag gggtggcgaa cgatgaggat tggctcgagg aaatcagaca gaccttctgt
     1381 attttggagc ccatctccgc gggtgagggc ttcaccttgg ccgagtatca agcctttgtc
     1441 gggtgtcagt tgtccagcac accagacacg tttgatacgg c
//
LOCUS       DATDUN010000816         1773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_243724, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000816 DATDUN010000000
VERSION     DATDUN010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243724"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VJC18_11355"
     CDS             complement(<1..552)
                     /locus_tag="VJC18_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HLD46020.1"
                     /translation="MLDIKWILQQPEAFAAYLQKRNMGAQADRITDLNDRRKALQKEF
                     DDLRALQNQKSKEIAQCKKENQSAEPVIAQMQEVASRVKEISAGQKEVEDNLNNLLLT
                     FPNVPHVSVPVGSSASENQEVRQWGTKPQFSFIPKEHGELGEKLGMLDFERAAKIAGA
                     RFAILSGKLAKLERALIGFMLDTH"
     gene            complement(790..>1773)
                     /locus_tag="VJC18_11360"
     CDS             complement(790..>1773)
                     /locus_tag="VJC18_11360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HLD46021.1"
                     /translation="NGEEEFLEYLKENLGIKAHQPLEPKLGHYPEVPDFQAETSDGKT
                     IRRSDYLGRVLHVIFFSPKCPHCQHELQFLRDQVYPEYHAKGYEVLAVNVAKMDEKTK
                     PLYDAQHYPWPLVDDSNRKLRTQFTFNRSVPENFLVDKQGRAHFYSVGYSEQRNDLYQ
                     MQIKKLLGLATEATMNPQRMATVKACEVCHEPESVDWAVSKHARALDSLAYKSEDRNP
                     ACVSCHTLGYGDPKGYQSMTDKKTGKQMAYVPPDLAGVQCESCHGIGGPHVKGAHAVT
                     VNEMKQKCLACHTKEWSSGFDVDKAMTQVNHSRAAEIMKMSVEEKEKMVRE"
BASE COUNT          395 a          486 c          395 g          497 t
ORIGIN      
        1 gtgggtatcg agcatgaatc caatcaaagc ccgctccagt ttagccaact tgccagacaa
       61 aatggcaaac cgggcaccag caatttttgc cgcgcgttca aagtccaaca tcccgagttt
      121 ttctcccaat tcaccatgtt ctttgggaat aaaagaaaac tgtggtttgg tgccccattg
      181 acgcacttcc tgattttctg acgccgagga ccccaccgga acagacacgt gtggaacgtt
      241 cggaaaagtt aacaaaagat tattaagatt gtcttcgact tctttttggc cggcagaaat
      301 ctccttgacg cgactggcga cctcctgcat ctgggcaatg accggctcgg cagactggtt
      361 ttcttttttg cattgggcaa tttcttttga tttttgattt tgcagggcac gcaggtcatc
      421 aaattccttt tgcaaggcct tgcgccgatc gttcaggtcc gtgatccggt ccgcctgcgc
      481 gcccatattc cttttttgca gatacgccgc aaaggcctct ggttgttgta atatccattt
      541 gatgtccaac atatgcgtca ctccgttctt cccacctacg ctctccgagc tacagcggac
      601 aagcgtggat cgtggatcgt ggatcgaaca cagctgaaag ctgaaaggtt tggggactgt
      661 catcccagcg gatgctggga tccatgtttt tggcaactct ggattccagc cttcgctgga
      721 atgacaaatc aaatattcca tttgagcttt caactttgat ctactttcat ccctcgtaag
      781 ctcattcccc tattccctta ccatcttctc tttttcctca acactcatct tcatgatttc
      841 ggcggcgcgg ctgtgattca cttgcgtcat agccttgtcg acatcaaacc ccgaagacca
      901 ttctttggtg tgacaggcca gacacttctg cttcatttcg ttcacagtca cagcgtgtgc
      961 ccccttgaca tgtggtccac caatcccgtg acaactttcg cactgcacgc ccgccaaatc
     1021 aggtggcaca taggccatct gtttacctgt ttttttatcg gtcatcgact gatatccctt
     1081 gggatcgcca taccccaacg tgtgacaaga cacgcacgcg gggttgcggt cctcgctctt
     1141 gtacgccagg gaatcgagtg cccgtgcgtg tttactgaca gcccaatcca cagactccgg
     1201 ctcatgacac acctcacacg ccttcactgt agccatgcgc tggggattca ttgtcgcctc
     1261 ggtagcgagc cccaaaagtt ttttgatctg catctgatac aagtcgtttc tttgttccga
     1321 ataacctacc gaataaaaat gggctcgacc ttgtttgtct accaggaaat tttcgggcac
     1381 agaacggttg aacgtgaatt gcgttctgag ttttcggttc gaatcgtcca ccaaaggcca
     1441 gggataatgt tgcgcgtcgt aaagtggttt ggttttttca tccatttttg ccacattcac
     1501 cgccaacacc tcgtacccct tggcatgata ttcgggataa acctggtcac gcaaaaactg
     1561 cagttcatgc tgacaatgcg gacacttggg agaaaaaaag ataacgtgta acacacgacc
     1621 caaataatcg ctccgcctga tggttttgcc atcgctggtc tcggcctgaa aatcagggac
     1681 ctctggataa tgccccaact tgggttccag cggttggtgt gctttgatgc caagattttc
     1741 cttgaggtac tccaggaatt cttcctctcc att
//
LOCUS       DATDUN010000817         1181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_162220, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000817 DATDUN010000000
VERSION     DATDUN010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162220"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /gene="pepT"
                     /locus_tag="VJC18_11365"
     CDS             <1..1086
                     /gene="pepT"
                     /locus_tag="VJC18_11365"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HLD46022.1"
                     /translation="SLGLTDANIDEKGFLFATLPTNCNRKKTIGLLAHIDTSAEASGT
                     NVNPQFHKNYTGQDIVLTNNITISPTDYPELLKCLGDTIITADGTTLLGADDKAGIAE
                     ILAALEFFKQHPEIQRPHIRVGITPDEETGSGVANFDVARFGADCAYTLDGSFTGDLC
                     YETFSANKGVITITGLNTHPGYAKDKMISSIRYAYRFLEELPSDQRPEKTNGYQGFFH
                     PYQIEGGVGKTTITMLLRDFTEEGLEEKTRILNEVKEKILKEEPKLQIDVAITKQYRN
                     MKDHIQARPEVLEMAKKAMLATGIEPDIRPIRGGTDGSSLSERGLPTPNIFAGFEAYH
                     GPKEWISTKVMGLAVCHIVNLMQLWAK"
BASE COUNT          334 a          335 c          274 g          238 t
ORIGIN      
        1 agcttgggcc tgacagatgc caacatcgat gaaaaaggtt tcctgtttgc aaccctgccc
       61 actaactgca acagaaaaaa aaccattggg cttttggcgc acatcgacac ctcagcagag
      121 gcgagtggca ctaacgtcaa cccgcaattt cacaaaaact atactggcca agacatcgtg
      181 ctcaccaaca acatcacgat ttcacccacg gactatcccg aactcctcaa gtgccttggt
      241 gacaccatca tcaccgcaga cggcacaacg cttctgggtg ccgatgacaa ggccggcata
      301 gccgaaattc tggcggctct cgaatttttc aagcaacacc ccgaaattca acgtccccat
      361 atccgcgtgg gcattacccc agatgaagag acgggctctg gtgtggcaaa ttttgatgtg
      421 gccagatttg gggctgattg cgcctacaca ctggacgggt cttttacagg tgacctgtgt
      481 tacgaaacct tcagtgccaa caagggagtg atcacgatca ccggcctcaa cacacacccg
      541 ggttacgcca aagacaagat gatcagttcc atccggtatg cctaccgttt tctcgaagaa
      601 ctcccctcag atcagcggcc cgaaaagaca aacggctatc aaggtttttt ccatccctac
      661 cagattgagg ggggcgttgg caaaaccacc atcaccatgt tactacgcga ttttaccgaa
      721 gagggcctcg aggaaaaaac acgcatcttg aatgaggtca aagaaaaaat cctcaaggaa
      781 gaacccaaac tgcaaatcga tgtcgctatc accaagcaat accgcaacat gaaagaccac
      841 attcaggcca ggccagaagt gctcgagatg gccaaaaagg ccatgctggc cacaggcatt
      901 gaaccagaca tccgcccgat ccgcggaggc acagacggtt cctctctttc cgagcgtggc
      961 ctgcccactc ccaacatttt tgcgggcttt gaagcctatc acggacccaa ggaatggatt
     1021 tccaccaagg tcatgggatt ggctgtgtgc cacatcgtca acctcatgca gttgtgggcg
     1081 aaataaaaag agcgtggatc gtggatcgtg aatcgtgcat cgtggatcgc aaaacacaat
     1141 attttcgaca cacgattgac gacacacgat ccacgttgca t
//
LOCUS       DATDUN010000818         3650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_327378, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000818 DATDUN010000000
VERSION     DATDUN010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327378"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VJC18_11370"
     CDS             complement(<1..541)
                     /locus_tag="VJC18_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HLD46023.1"
                     /translation="MDKQPHDKFASPHKRFDRAFFESSDDFTIIGNGEIGGKAHGLLL
                     AKDVLIKTFPSGSFEGITIGIPKLTVITTEYFDDFMNRNHLHDVTLSTLSDEEIALRF
                     QQTDLSPNLVGDLWTLIAKAHTPLAVRSSSLLEDAKFEPFAGVYATKMIPNNQPDTQK
                     KFQVLIEAIKFVYASVFFSQ"
     gene            complement(557..1954)
                     /locus_tag="VJC18_11375"
     CDS             complement(557..1954)
                     /locus_tag="VJC18_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HLD46024.1"
                     /translation="MTTTGLIIAAGAGTRLADETQVPKPLRKVAGLPLLKRIILTAAK
                     AGLTKMIVVVGFEKEKITTFITSQKWPLPVECVENKDWKKSNGLSVLAAKDVLKENFV
                     LLMSDHVFDVHTLQKLIKHGLGDAQARLAVDYKVHQIFDQDDATKVATRNDKVVAIGK
                     NLIEYDAIDTGMFLLSPAIFDALAEAKKDGDCSLSDGIRTLAAKGHMGIFDIGDGFWQ
                     DVDTPACLKHAENVLFNACRKPTDGIVSRNFNRHVSLFISKRLVNTPLTANQITFIVL
                     LLGLLSGFLASLGTYTTYLLAAILFKLTSILDGVDGEISKVKLTSSKFGQWLDTLCDN
                     TTYIVFLLGTIVGLFRQHYPYSVVLSLFAGVGLLIALSIVFWHVLKHSNSGSLLALQK
                     DFQTRTDLTLLSRIFFKLHFAIKRDFFSVAFLVFALFGKPQWVMLSTAIAMNLTWIII
                     LQQQMTTLSLAKKKT"
     gene            complement(2131..>3650)
                     /locus_tag="VJC18_11380"
     CDS             complement(2131..>3650)
                     /locus_tag="VJC18_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46025.1"
                     /translation="FYPTTIAQNVADVVPLLSTISDNNIIGTPPQIRTTNPHYSLPIT
                     HYPSSLRPSSLRPAPASPLLSFSQDPTSDERIPQFLYEEDPEENEEAFGTVKETRASP
                     KDASAKKTYPSAYKNTLSSKPSDVVLDTTNPEGTKNYHALPELLVPRYLLPGLLYYEN
                     SFLVSAAIGRTDPLYRHSWSAFANYRSDAAFVGGGGTYMFSRYKPLFYVGGLRYAVDW
                     GTINGINFFEERNQAYAGMSWGIKAHRFNLAYFYEHRSALTNLNVNLINMMPYAGSRF
                     QYTFSKYKMYADSISPENGVVFKVGGEWTDSMLGSDAVNEEIALRGDLRFYLEMPWSD
                     HHVLALRTAVGWVWGDQQQFGDYRLGGPFGEGTGASYSSRVFPLRGLAGITYGGDYAY
                     MFSSEYRIPLATNVNWGIGTWPIFLDKLYMALFVDGGDIKYRTETNELFSRMLVSTGA
                     EVNGNFVLGYGLPLTIRAGYGVILTNRWRLGTLTDSITMMSLKYGSVYLQFGTMF"
BASE COUNT          945 a          854 c          975 g          876 t
ORIGIN      
        1 cctgcgaaaa aaaaacggag gcataaacaa atttgatggc ttcaataagg acctggaatt
       61 ttttttgcgt gtcgggctga ttgttgggga tcatcttggt ggcataaaca ccagcaaaag
      121 gttcaaattt tgcatcttcc agcaagcttg aagaacgaac ggccagcggc gtgtgagctt
      181 ttgcgatcag tgtccacaga tcacccacga gattggggga gagatctgtt tgttgaaaac
      241 gcaaggcgat ttcttcatct gacagggtgg ataaagtcac atcatgcaaa tgatttctgt
      301 tcatgaagtc gtcaaaatac tcagtggtga ttacggtgag cttggggatg cctatcgtga
      361 tcccttcaaa tgaacccgag ggaaaggtct tgatcagaac gtcttttgcc aacaagagcc
      421 cgtgtgcttt tccgccaatt tcgccgttgc ctatgatggt aaaatcatcg gaggactcaa
      481 aaaaggccct gtcgaatctc ttgtggggtg aggcgaattt gtcatgaggc tgtttgtcca
      541 tgctgtgttt ccctgctcat gttttcttct ttgccaaaga aagcgttgtc atttgttgtt
      601 gcaaaataat gatccacgtc agattcatgg caatggctgt ggaaagcatg acccattgcg
      661 gctttccgaa tagcgcaaaa accaaaaaag cgaccgagaa aaaatcacgc ttgatggcaa
      721 aatgcagttt gaaaaaaatc cgtgacaaca aggtcaggtc tgttctggtc tggaaatcct
      781 tttgcagggc caagagacta cccgaattgc tgtgtttgag gacatgccaa aacacaatgc
      841 tgagcgcgat caaaagaccg acccccgcaa agaggctgag cacaacgctg taagggtagt
      901 gctgtcgaaa caggcccacg atggttccca acaggaacac aatgtaagtg gtgttgtcac
      961 aaagggtgtc tagccactga ccaaattttg aactggtgag cttgaccttt gaaatttcgc
     1021 catcaacccc atccagaatg gaggtgagct tgaacaggat cgccgccaac aagtatgtgg
     1081 tgtaggttcc cagagaagcc aaaaaacctg ataacagccc caacaacaac acaatgaacg
     1141 tgatctggtt ggcggtgagc ggcgtgttga ccagtctctt ggaaataaac agggacacat
     1201 ggcgattaaa gttgcgcgac acaatcccgt cggtgggttt gcggcacgcg ttaaaaagaa
     1261 cattttcggc gtgtttcagg caggcagggg tgtccacatc ttgccaaaag ccatcaccga
     1321 tgtcgaagat gcccatgtgg ccttttgctg ccagcgtcct gatgccatcg gagagagagc
     1381 aatccccgtc tttttttgcc tcagcgagcg catcaaaaat ggcggggctg agcaaaaaca
     1441 tgccggtatc aatggcatca tattcaatga gatttttgcc aatcgccaca accttgtcgt
     1501 ttcgagtggc caccttggtg gcgtcgtcct gatcgaaaat ttgatggacc ttgtaatcca
     1561 ctgccaatct ggcttgggcg tcacccaggc catgtttaat gagtttttga agtgtgtgca
     1621 catcaaaaac gtggtcactc atcaacaaaa caaaattttc cttaaggaca tctttggcgg
     1681 ccaatacaga aaggccattg gactttttcc agtctttgtt ttcaacgcat tccactggaa
     1741 ggggccattt ttgactcgtg atgaaggtgg tgattttttc cttctcaaaa cccacaacga
     1801 cgatcatttt ggtgaggcct gccttggcgg cggtgaggat gattcgtttg agcaacggga
     1861 gtccggcgac tttgcgcaaa ggttttggca cttgtgtttc atccgcaaga cgcgtgcccg
     1921 cgccagcggc aatgatgagg ccagtggttg tcatggtgtc ttttgctcct tccaaagcaa
     1981 gtgtcattcc tgcggaagca ggaatccaga attgtagaag atatggatcc cagcgttcgc
     2041 tgggatgaca agtccaaatc ccgcttcacc ttgtaccata atggcacagg ggatcaccca
     2101 aaaaaaatgc tggtaggttt tcgaaaaccc ttaaaacatc gtcccaaatt gcaggtagac
     2161 actgccgtat ttgagactca tcatggtgat ggagtctgtc agggtgccca gacgccacct
     2221 gttcgtgagg atgaccccgt agccagccct gatcgtcagg ggcagaccgt accccaacac
     2281 gaaattcccg ttgacctcag caccggtgga gaccagcatg cgcgaaaaca gctcgttggt
     2341 ttcggtgcgg tatttgatgt ccccgccatc cacaaacagc gccatgtaaa gtttgtccaa
     2401 aaagataggc caagtcccga tgccccagtt gacgttggtg gccaaaggaa tgcggtactc
     2461 tgacgaaaac atgtaggcat agtcaccgcc ataagtgatc ccagccaaac cgcgcaaagg
     2521 gaacacgcgc gaagaataac tggcaccggt gccttcgcca aaaggccccc ccagacggta
     2581 gtcaccaaat tgttgttggt ctccccaaac ccagcctact gcggtcctca gggccaacac
     2641 gtgatgatcc gaccaaggca tttcgagata gaacctcaga tcaccccgca gggcgatttc
     2701 ttcgttgacc gcgtcggaac ccagcatcga gtctgtccac tccccgccca ccttgaaaac
     2761 cacaccattt tcggggctga tggaatcggc gtacatttta tatttggaaa acgtgtactg
     2821 aaaccggctg cccgcataag gcatcatgtt gatgaggttg acgttgagat tcgtgagcgc
     2881 tgaacgatgt tcataaaaat aggccaggtt aaaacggtga gccttgatgc cccaagacat
     2941 tccggcgtag gcctgattgc gttcctcaaa aaaattgatg ccgttaatgg tcccccaatc
     3001 gacagcataa cgcaggccgc ccacgtaaaa gagaggtttg tatcgggaga acatgtacgt
     3061 gccgccaccc cccacaaagg cggcatcaga gcggtaattg gcaaaggcgg accaggagtg
     3121 cctgtacaaa ggatcggttc gaccaatggc cgccgaaacc agaaaagagt tttcgtaata
     3181 caagagtccc gggagcaggt aacgggggac caggagttct ggcagggcgt gataattttt
     3241 tgtgccctcc ggatttgttg tgtcgaggac gacatccgat ggttttgagc tcagagtgtt
     3301 tttataagcg gaggggtatg tttttttggc ggaggcgtct ttgggggatg ctctggtttc
     3361 cttcactgtg ccgaaggctt cttcattttc ctcaggatcc tcctcgtaca aaaattgcgg
     3421 aattctttcg tccgaagtgg ggtcttggct gaaagaaagc aaggggctgg caggggcggg
     3481 acgaagggac gacggacgaa gggacgaagg gtaatgggta atgggtaatg agtaatgggg
     3541 gttggtagtc cgtatctgtg gtggtgtgcc gatgatgttg ttgtcagaaa ttgttgataa
     3601 tagagggacg acatcagcta cgttttgggc aattgttgtt gggtaaaact
//
LOCUS       DATDUN010000819         1571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_410166, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000819 DATDUN010000000
VERSION     DATDUN010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410166"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VJC18_11385"
     CDS             complement(<1..399)
                     /locus_tag="VJC18_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLD46026.1"
                     /translation="MTNKLISISINNNNVQADPNKTILATARENNIHIPTLCHDDRLK
                     PIGSCLLCVVAIEGQNKLALACTTKPADGMKITTHNEKISTSRKQALELLLSNHYADC
                     RGPCFDNCPAHVDVQGYLALAHEGKYEEAIN"
     gene            complement(420..>1571)
                     /locus_tag="VJC18_11390"
     CDS             complement(420..>1571)
                     /locus_tag="VJC18_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HLD46027.1"
                     /translation="SVLEGMLICGYAIGSHYGYIYCRAEYPKAISKLKQAIKLATEHG
                     FLGENILGSSFSFHIKIKEGAGAFVCGEETALIASIEGHRGTPRVRPPYPAASGLWGK
                     PTNINNVETFANIAWIILNGSEAFANIGTADSKGTKVFAMAGKVKRSGLVEVPMGITI
                     HDIVFGICGGIVGDKKFKAVQMGGPSGGCVPASLAETPIDYQRINETGAIMGSGGMIV
                     VDETVCMVELARFFLEFTQKESCGKCTSCRIGTLRMLEILENIVSGKGKPSDIDDLIQ
                     LGEQIKATSQCGLGQTAPNPVLTTIRYFREEYDAHIQDKRCPAHGCAQLVNYIITPDL
                     CTGCGICLRDCPSQAITGTKKTPHSIDTVKCSKCGKCITACHFNAINKN"
BASE COUNT          344 a          380 c          444 g          403 t
ORIGIN      
        1 gttgatggcc tcttcatact tgccctcgtg ggccagcgcc agataaccct gaacgtccac
       61 atgcgcggga cagttgtcaa aacaggggcc gcgacaatct gcataatggt tcgacaacag
      121 gagttccagc gcctgtttgc gagaggtgga gattttttca ttgtgtgtgg tgatcttcat
      181 gccgtcagca ggcttggtgg tgcaggccaa ggcaagtttg ttttgaccct caatggcgac
      241 cacacaaagc agacaagacc caatgggttt gagtcggtca tcgtgacaca gtgttggaat
      301 gtgaatgttg ttttcgcgtg ccgtggccag gatagtcttg tttggatcgg cctgaacgtt
      361 attattgttg atgctgatgg atatgagttt gttagtcatg acttccccta agagctgccc
      421 taatttttgt taatggcgtt aaaatgacag gccgtgatgc acttgccgca tttactgcat
      481 ttgacggtat caatactgtg gggcgttttt tttgtgcccg tgatggcctg tgaaggacaa
      541 tcacgcagac aaatgccgca accggtgcaa agatcgggag tgatgatgta attgacaagt
      601 tgtgcgcaac catgggctgg gcaacgtttg tcttgaatat gcgcgtcgta ttcttccctg
      661 aaataacgga tcgtggtcag cacggggttg ggtgcggttt gccccaaacc gcattgggag
      721 gtggccttga tctgttcgcc cagttgaatg agatcgtcga tatctgaggg tttgcctttt
      781 ccggagacaa tgttctcgag gatctccagc attctcagtg tgccgatgcg gcaggaggtg
      841 catttgccgc aggactcttt ttgtgtgaat tccagaaaaa aacgggcgag ttccaccatg
      901 cacacggttt cgtccacgac aatcatgcca ccagaaccca tgatggcacc ggtttcgttg
      961 atgcgttgat aatcgatggg ggtctctgcg agagaggcgg gcacacaacc gccagagggt
     1021 ccgcccatct gcacggcctt gaattttttg tctcccacaa tcccgccgca gatgccaaac
     1081 acgatgtcat ggatcgtgat gcccatgggg acttcaacca ggcccgatct ttttaccttg
     1141 ccggccatgg caaagacctt ggtgcccttg ctatcggctg tgccaatgtt ggcgaaggcc
     1201 tcagagccat tcaaaatgat ccacgcaatg ttggcaaagg tttccacgtt gttgatgttg
     1261 gtgggtttgc cccacaaacc gctggcggca gggtagggcg ggcgtacgcg cggagtgccg
     1321 cggtgtcctt cgatcgaggc aatcaaggca gtttcttcgc cgcacacaaa cgctccggca
     1381 ccctccttga ttttgatgtg gaacgaaaaa gaggaaccca aaatattttc acccaaaaag
     1441 ccgtgttctg tggctagctt gatggcctgc tttagtttgc tgatggcctt ggggtattcg
     1501 gcgcggcaat aaatataccc ataatgagag ccgatggcgt aaccgcaaat cagcatgcct
     1561 tcgaggaccg a
//
LOCUS       DATDUN010000820         1192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_327582, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000820 DATDUN010000000
VERSION     DATDUN010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327582"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VJC18_11395"
     CDS             <1..369
                     /locus_tag="VJC18_11395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HLD46028.1"
                     /translation="DIIKNAGISDLKGVVHCFSGNLDLARKYLDLGFMISFTGIITFP
                     KAQELRDVLKYVPLDRIMLETDAPFLTPHPHRGKKNQPAYVKYVAERMAEIKQISFEE
                     VSRLTTQNAELFFGLGDVVD"
     gene            complement(349..882)
                     /gene="def"
                     /locus_tag="VJC18_11400"
     CDS             complement(349..882)
                     /gene="def"
                     /locus_tag="VJC18_11400"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLD46029.1"
                     /translation="MSLLTILTYPTKSLKEPSVPVKKVTAEIQTLVHDLFETMQGTTG
                     IGLAAPQVGNNITLFVMDVPMPLPDDAEQTTSRPLCFINPQIVHQLGTTTYEEGCLSC
                     PDLLVNRERASEIDVEALDADGKEFTIHLTGLEAICAQHEMDHLKGILLVDHLSRLKR
                     DMYRKQMVRRRLVNNVP"
BASE COUNT          275 a          337 c          273 g          307 t
ORIGIN      
        1 gacatcatca aaaacgcggg aatttccgac ctcaaagggg tcgtgcattg tttttcgggc
       61 aatctggatc tggccaggaa atatctggat ctggggttca tgatttcttt tacgggcatc
      121 atcacgtttc ccaaggccca ggagctacgc gacgtgctca agtacgttcc cctagaccgg
      181 atcatgttgg aaacagatgc ccccttcctc acaccgcacc cgcatcgcgg caaaaaaaac
      241 cagcccgcgt acgtcaaata tgtggccgag cgcatggcag agatcaaaca aatttctttt
      301 gaagaagtct ctcgcctcac cacgcaaaat gcggaactgt tttttggttt aggggacgtt
      361 gttgactaac ctccgcctca ccatttgttt gcgatacata tcccttttaa gacgtgacag
      421 atggtccaca agcaaaatgc ccttgaggtg atccatctcg tgttgcgcgc agatggcctc
      481 cagcccagtg aggtgaatgg taaactcttt gccatcggca tcaagcgcct ccacatcaat
      541 ttcactggcc cgctccctat tcaccagcag atccgggcag gacaaacaac cttcttcgta
      601 tgtggtggtc cccaactgat gaacaatctg tggattgata aaacagagcg ggcgcgacgt
      661 tgtctgctcc gcatcatcgg gaagaggcat gggcacatcc atcacaaaca acgtgatgtt
      721 gttcccgact tgcggtgcgg ccagtccgat tccggtcgtc ccctgcatgg tttcgaacag
      781 gtcatgcaca agtgtctgaa tctccgccgt gaccttcttg acgggaacag acggttcttt
      841 gagacttttt gtggggtagg tcaggattgt gagtagtgac ataagaaggt tcattctaca
      901 gcaaaccaaa tcaccatttc aaccttttcg tggttcgctt gtcatttgat attttgcact
      961 tttcactttg cactttgcat tgacaaaatc ccctcgtcca ttcgtccctc gtgtcttcgt
     1021 ccctcatccc ctacaccatc cccactgcgt ccacgtcgat ctggtattga atgcccctga
     1081 gtttgcgccg atcaagatcg tcccgcagat aggccaccaa atccccaacc ttgagtcccc
     1141 ggcgaatttt taaaatcacg tggtggcgat acttgccgcg aacctttgcc ac
//
LOCUS       DATDUN010000821         1025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_576496, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000821 DATDUN010000000
VERSION     DATDUN010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576496"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1025
                     /locus_tag="VJC18_11405"
     CDS             <1..>1025
                     /locus_tag="VJC18_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HLD46030.1"
                     /translation="GNTALKEGQVLDEEEYQNARAEHGGDFDAGMGGKSIQEMLRRLD
                     LDELYKKVTLQLAKTESETTKKKLSKRLKVIISFRESGNRPEWMMLEVVPVIPPDLRP
                     LVPLEGGRFATSDLNDLYRRVINRNNRLQRLQELNAPEIIVRNEMRMLQEAVDALLDN
                     GRRGKVFTGASKRPLRSLSDMIKGKQGRFRLNLLGKRVDYSGRSVIVVGPDLKLHQCG
                     LPKKMALELFKPFIYHKLERRGLVTTIKSARKMVEKEHPEVYDVLEEVIKEHAVLLNR
                     APTLHRLGIQAFEPILIEGKAIRLHPLVCVAFNADFDGDQMAVHVPLSFEAQLEAQVL
                     MLSTNNI"
BASE COUNT          256 a          236 c          284 g          249 t
ORIGIN      
        1 aggcaatacc gccctcaaag aagggcaagt actggatgag gaagagtatc agaatgcgcg
       61 cgccgagcac ggtggtgatt tcgacgcagg catgggcggc aagtccattc aggaaatgtt
      121 gcggcgtctc gatttggatg agttgtataa aaaggtcact cttcagttgg caaagacaga
      181 gtccgaaacg acaaaaaaga aattgtccaa gagacttaaa gtcatcattt cgttcagaga
      241 gtcaggcaat cgtcccgagt ggatgatgct cgaagttgtg cccgtcattc ctcccgattt
      301 gcgacccttg gtgcctctcg aaggtggtcg ttttgccaca tcagacctca acgatttgta
      361 tcgtcgcgtg attaaccgga acaaccggtt gcagcgtttg caggaactca atgcccccga
      421 aatcattgtg cgtaacgaaa tgcgtatgtt gcaggaagct gtggatgccc ttttggataa
      481 tggacgtcgg ggcaaggtgt tcacaggggc cagcaagaga cctttgcgtt ccttgtccga
      541 catgatcaag ggcaaacagg gacgttttcg actcaatttg ttgggcaaac gtgtcgacta
      601 ttccggtcgt tctgtgattg ttgtggggcc agatcttaag ttgcaccagt gtggtttgcc
      661 caaaaaaatg gctttggagt tattcaagcc gttcatttat cacaaattgg agagacgagg
      721 tttggtgacc accatcaaga gtgctcgcaa gatggtcgaa aaagagcacc ccgaagtgta
      781 tgatgtgttg gaagaagtca tcaaggaaca cgcagtgctt ctcaaccgcg ctcccacatt
      841 gcatcgtttg gggattcagg cctttgagcc catcttgatc gaaggtaaag cgatcaggtt
      901 gcacccgttg gtatgcgtgg ccttcaacgc cgacttcgat ggtgaccaga tggccgtaca
      961 cgtgccactg tcattcgaag ctcagctcga ggctcaggtg ctgatgctgt cgaccaacaa
     1021 tatcc
//
LOCUS       DATDUN010000822         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_327851, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000822 DATDUN010000000
VERSION     DATDUN010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327851"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..854)
                     /locus_tag="VJC18_11410"
     CDS             complement(81..854)
                     /locus_tag="VJC18_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HLD46031.1"
                     /translation="MSIEILAESRPAIGKSYSKKLKKEKKIPAVLYGREMTNKTLQVV
                     AHDIDKVLLSEKGMNTLINLNVKDDKVYTALIKDMNGDPVTRALNHVDFWAVKAEQEV
                     HVHVEIKLEGKAPGQLQGGIVEQVSHQVLLYCRADAIPNFISVDIGTLEIGHNIHLNE
                     VKLPEGVRAKEGYNPTIVAVVEEKKIEEVVPVAAEGVEGAVPAEGAAPAEGAVAAPAD
                     GKAPAAADGKAAPGAAPAAKAGADKGAGKGGDSKKDKKK"
     gene            complement(1087..2034)
                     /locus_tag="VJC18_11415"
     CDS             complement(1087..2034)
                     /locus_tag="VJC18_11415"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HLD46032.1"
                     /translation="MSRFKDILLFTGSANPKLAQAISRTLDIPLADTVLKRFSDGEIW
                     VEIQENVRQKDAYIIQPTCEPANENLMELLVMIDALKRASVDTVTAVIPYYGYARQDR
                     KSQPRVPITAKLVADLLAAAGTDRVVSIDLHAGQIQGFFDIPFDHLFAMPVFLEHIKK
                     TFVEEDLLVVSPDVGGAERARSYAKRLGCNIALIDKRRPKPNVSEVMHVIGEVEGKNC
                     LIVDDIVDTAGTLTAGAKTLLEYGAKSVSALCSHGVLSGPAIERIKDSVLEKLIISDT
                     IPLSKEKQISKIEQVTVAPILSKGILRIAKGQSVSSLFV"
BASE COUNT          486 a          561 c          489 g          509 t
ORIGIN      
        1 caacagggat atccttgttg accgttagcc gttgaccttt ggccgttagc tgttagccgt
       61 agaccgttgg catgaaatcc ctatttcttc ttgtcttttt tagaatcacc gcccttgcca
      121 gcacccttgt cggcaccagc ttttgcagca ggagcggccc cgggggcagc cttgccatcc
      181 gcggcggcag gagccttgcc atcagcagga gcggcaacag caccttcagc aggagccgca
      241 ccctcagcag gaacagcgcc ttcaacgcct tcggcggcca cgggaacgac ctcttcaatc
      301 ttcttctctt caacaacggc cacgatggtc gggttatagc cttctttggc acgcacacct
      361 tccggcaact taacctcgtt caagtgaatg ttgtgtccaa tttccaaggt gccaatatca
      421 acagaaatga aattgggaat ggcatcggca cggcaataca acagaacctg atgcgaaacc
      481 tgctcaacaa tcccgccctg caactgaccg ggggccttgc cttcgagctt gatttcgacg
      541 tgcacatgca cttcctgctc cgccttgacc gcccagaaat caacgtgatt caaggcgcgg
      601 gtaacgggat cgccattcat atccttaatg agcgcggtgt aaaccttgtc atccttgaca
      661 ttgaggttaa tcaaagtgtt catccccttt tcggacagca acactttgtc gatatcgtgt
      721 gccacaacct gcaacgtttt gttggtcatt tcacgaccgt aaagtacggc agggatcttc
      781 ttttcttttt ttaatttttt ggaataactt ttgccaatgg cgggcctgga ttcggccaga
      841 atttctatgg acatattttg ttcctccagc ccatcggtcg cgtgtcatga caccccaaac
      901 ctacttgctg aatggattat tttttgaggg gcccttgtag taaaagataa agcaaaaagc
      961 aacaccacaa cgcgttattt cggacttcgt tgcgtatctg caagggggat cgccacattc
     1021 ccgttggtca gtcgcgatga caaccttgca atttcagccg gttacagaaa ccacactgaa
     1081 tattattcaa acaaatagcg atgatacact ttgacctttg gcaatacgca aaatcccttt
     1141 gcttaatatg ggggccacgg tcacctgttc gatcttgctg atctgttttt cctttgagag
     1201 gggaatggta tcagaaatga tgagtttttc gagcaccgaa tctttgatgc gctcaatggc
     1261 cggacccgac aacacaccgt gagagcagag tgcagagact gacttggcac cgtattccag
     1321 aagcgttttg gccccggcag tgagcgttcc cgcagtgtct acaatatcgt ccacgatcag
     1381 acaattcttc ccttcgacct caccgatcac gtgcatcact tcggacacgt tcggtttggg
     1441 acgacgtttg tcaatgaggg caatgttgca acccaagcgc ttggcataac tgcgcgcgcg
     1501 ctccgcccca cccacatcgg gagacacgac caagagatct tcttcgacaa acgttttttt
     1561 gatatgctct aaaaaaacag gcatcgcaaa aaggtgatca aaagggatat cgaaaaaacc
     1621 ctgaatctgc cccgcgtgca gatcgatgga caccacacgg tctgtgcccg ccgccgccaa
     1681 caaatcggcc accagcttgg ccgtaatggg aacgcgtggt tgcgatttgc ggtcctggcg
     1741 cgcgtagccg taataaggga tcacggctgt caccgtatcg accgaggctc tcttgagggc
     1801 atcaatcatg accaatagct ccatgagatt ctcattggcc ggctcgcatg tcggctgaat
     1861 gatataggcg tccttttgac gcacattttc ctgaatctcc acccagatct ctccgtcaga
     1921 aaaccgcttg agtaccgtgt ctgccagtgg gatatcgagt gtgcgcgaaa tggcctgggc
     1981 cagtttggga ttggcggaac cggtaaacaa caaaatgtct ttaaaacgac tcatgggatc
     2041 tcctt
//
LOCUS       DATDUN010000823         1494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_619168, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000823 DATDUN010000000
VERSION     DATDUN010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619168"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VJC18_11420"
     CDS             complement(<1..344)
                     /locus_tag="VJC18_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD46033.1"
                     /translation="MKLIGRQEQLKQFRMFMESPRAELVAVYGRRRVGKTFLVREYFK
                     EQGTYFELTGIKDGSLEEQLLAFRDTFQKTFYPTVPVEVPKSWREAFLMLTREIERHE
                     QNRVIVFLDELP"
     gene            complement(1151..>1494)
                     /locus_tag="VJC18_11425"
     CDS             complement(1151..>1494)
                     /locus_tag="VJC18_11425"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162370.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HLD46034.1"
                     /translation="LVKNGCFAVSEGANMPTVPDGVDVFIENKILYGPGKAANAGGVA
                     TSGLEMSQNALHLNWTRQEVDGRLQLIMKSIHSACVQHGKVGQYVNYVKGANIAGFIK
                     VADAMLDQGVV"
BASE COUNT          394 a          387 c          302 g          411 t
ORIGIN      
        1 caaggcagtt catccaaaaa cacaatcact ctgttttgct catgacgctc gatttcacgc
       61 gtcagcatta aaaaagcctc tcgccaactt ttgggaacct ccacagggac tgtgggatag
      121 aaggtttttt gaaatgtatc cctgaaggcc aatagctgtt cctccaaaga accgtctttg
      181 atccccgtca gttcaaaata ggtcccttgt tccttaaaat attctctgac aagaaaagtt
      241 ttacccactc tgcgcctgcc ataaacagcc accagttccg cacgtgggga ctccataaac
      301 attctgaact gtttaagttg ctcttgacgg ccaatcaact tcataacaac ctcgctaatt
      361 ataatatatt tataactagc gatatagtaa cacacacgga atcattcaac aagaatattc
      421 tgattacatt acagcatgtt aattcgtact atgattgtat tgttatgaaa tatcatgtct
      481 cgctgattat aaactgtcta tatttagcga gaatttgtgg ctcgtttttc aaaagtttgc
      541 tcacaattgt catcttggaa gagaaaatga cgagcgtctg cattctcaat ccttttcagc
      601 cttggtggct gtgttggtga agggcacctc atatggatga cctgaatata ttcagcacga
      661 tcagcccaag cttgggatta ctgggaatcc gcaaccagtg tgaagaacag catcttatta
      721 caacatctta atacaaccgc ttgatacaac gtgcctgagt ctttaacccc gccataacat
      781 gttatcatta catgggatat tgtggaaata tgtgtaaaaa aagggccggt tatacgaaca
      841 ttatacgctt caacatttgt tgatgcaaat gatgccctga tggaaagtat cgcgttaccc
      901 tcaccatccc tcttggcgcc atcaaataaa accctaaaat ccttgtcctt tgtagattgc
      961 cacgcgacaa aaagtcgctc gcaatgactg atccctgccg ggcgcaccaa aaaaaaggac
     1021 gagaggtttt cccctcgtcc tttcgtcctt tcgcctgccc tgagcgagta tagcgattta
     1081 ttctgagcct gtcgaagaag tcgaagggtc catcgtcctt tcgtcctttc gtccatcgtg
     1141 ttttcgctcc tcacaccacg ccttgatcca gcatggcgtc ggccaccttg atgaaaccgg
     1201 cgatgttggc gcctttgacg tagttaacat actgcccaac tttaccgtgt tgcacacagg
     1261 ccgaatggat ggacttcatg atgagctgta aacgcccatc gacttcctgc cgcgtccagt
     1321 tgaggtgcag ggcgttctgc gacatctcca aaccgctggt ggccacacca cctgcattgg
     1381 ccgctttgcc cggtccgtaa agaatcttgt tttcaataaa aacatcgacc ccgtccggca
     1441 cggtcggcat gttggcccct tcgctcaccg caaaacaacc gttcttgacg agcg
//
LOCUS       DATDUN010000824         1057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_410638, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000824 DATDUN010000000
VERSION     DATDUN010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410638"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1057
                     /locus_tag="VJC18_11430"
     CDS             <1..>1057
                     /locus_tag="VJC18_11430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLD46035.1"
                     /translation="SAKGYGVRDYTSNAKVSKHTVFQLASLSKTFTSVLLGVLIDNKR
                     LSLDDPIVKFLPDLVLGSPEHTKKIKVRHLLNQTTGFPSHSGDVRIEGGESFDQIIKS
                     FSNLKILNGPGQVHSYQNVLYALAGKIIEEVTQMSFAQAVRYYLFTPLQMNDASVGRQ
                     AFKLAINRALPFRYIGGTHKQIPVSYDYYTVAGAAGVNASIADMAQWLNFTAGTIPLV
                     PLANMPVISLATLQSLQKPSVSSPAETSRMRPYRERVTRTHYGLGFRISDWAGHRIVY
                     HGGMVDGMKSILAFDPQKKAGIVILVNSNTPALGIIRSYFFDKLYGLNDIDWNQRFKQ
                     QELQAAQKKKARKKVVRK"
BASE COUNT          267 a          316 c          249 g          225 t
ORIGIN      
        1 tcggccaaag gctatggcgt gcgtgattac accagtaacg ccaaagtgag taaacacacg
       61 gtttttcagt tggcctcgct gtccaagacc ttcacctcag tcctgctcgg tgttttgatc
      121 gacaacaaga ggctctctct cgatgacccc atcgtaaaat tcctgcccga cctcgtgctc
      181 ggcagtcccg aacacacaaa aaaaatcaag gtgcgccatc tgctcaatca gaccaccggc
      241 tttcccagtc attcaggcga tgtccgcatc gaagggggag agagttttga ccagatcatc
      301 aaaagctttt ccaacctcaa gatcctcaat ggccccggtc aggtccacag ctatcaaaat
      361 gtcctctacg ccctcgctgg caagatcatc gaagaggtca cgcaaatgag tttcgctcag
      421 gcagtcaggt attacctctt cacccctctg cagatgaacg atgcctctgt gggcaggcaa
      481 gcctttaaac tcgccatcaa cagggccttg ccctttcgat acattggtgg cacacacaaa
      541 cagatccccg tgagttatga ttattacacc gtggccggag ccgcgggggt caacgccagc
      601 attgccgata tggcccaatg gttgaacttc accgccggaa caatccctct ggtgcctctg
      661 gccaacatgc ccgtcatcag tctcgccacc ctgcaatcac tgcaaaagcc gtctgtctct
      721 tccccagccg aaacgtcccg catgcgtccc tatcgcgaac gggtgaccag aacacattac
      781 ggcctggggt tcaggatcag cgactgggcc ggtcacagaa ttgtttatca cggcgggatg
      841 gtggacggca tgaagtccat tttggccttc gacccgcaaa aaaaggccgg catcgttatc
      901 ctcgtcaaca gcaacacacc cgcactcggc atcatccgca gttatttttt cgacaagctg
      961 tatggattga atgacatcga ttggaaccag cgcttcaaac agcaggaact gcaggccgct
     1021 caaaaaaaga aggcgcgcaa aaaagttgtt cggaaaa
//
LOCUS       DATDUN010000825        13828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_410661, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000825 DATDUN010000000
VERSION     DATDUN010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13828
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410661"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VJC18_11435"
     CDS             complement(<1..252)
                     /locus_tag="VJC18_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46036.1"
                     /translation="MTESPSSMIAMSMKRFSLLIISFLLFLPSRALAAPPQYRLNLSD
                     CIEMAVRNNEEIKAQEYDVQAYIARKIEATKRYVPVVTIK"
     gene            complement(298..1713)
                     /gene="hslU"
                     /locus_tag="VJC18_11440"
     CDS             complement(298..1713)
                     /gene="hslU"
                     /locus_tag="VJC18_11440"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HLD46037.1"
                     /translation="MNEIKNYTPKQIVQELDRYIIGQHEAKRSVAIALRNRWRRLNVK
                     GDMQDEIIPKNIIMIGPTGVGKTEIARRLAKLAESPFYKVEASKFTEVGYVGKDVESM
                     IRDLTEIGVNMIRQREQECVKGKAKDLAEERLLDLLLPIKKKKAPPVDMTMTPVRIEE
                     TTVPEEEDTSRTREKLRQMLRDGKLNDRKVDLDIGPKPPAFTGGPGVPMVEIISAGGS
                     LDDISSNLRDMFGGMFQQGKKRKKVPVPEALEILTHEEAAKLIDMETVTKMAVSFVEN
                     NGIIFIDEIDKIASRSDLRGGPDVSREGVQRDILPIVEGSTVSTKYGFVKTDHILFIA
                     SGAFHVSKPSDLIPELQGRFPIRVELNSLTKHDFLRILTEPQNSLTKQYKALMGSENV
                     TLQFTDDALEEIASFAAKANEQMENIGARRLHTILEKVMEEVSYDANERAGTTFEITS
                     PYVQKCLVKIIDNQDLSKYIL"
     gene            complement(1807..2346)
                     /gene="hslV"
                     /locus_tag="VJC18_11445"
     CDS             complement(1807..2346)
                     /gene="hslV"
                     /locus_tag="VJC18_11445"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HLD46038.1"
                     /translation="MYETHATTIIAIKKGNSVAMAGDGQVSLGNTILKGKAKKVRRMN
                     EDKVIAGFAGSTADAFTLFERFEAKLKEYSGNLKRAAVELAKDWRTDKVLRRLEALLI
                     VADKESLFTISGNGDVIEPDDGIAAIGSGGMFALAAARALMTHTKLSVKQIAEESMKI
                     AGDICIYTNHNITLESIEE"
     gene            complement(2464..3372)
                     /gene="xerC"
                     /locus_tag="VJC18_11450"
     CDS             complement(2464..3372)
                     /gene="xerC"
                     /locus_tag="VJC18_11450"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HLD46039.1"
                     /translation="MTVEKNIEEFMTYLMNQKNAAENTILSYRRDLTEFYHYLLNHQA
                     EVISDNAMLLDRINPLMIRSYLSLLFQKNGASSIARKLSALRSLFKYFVKKGTIDQNP
                     AKVINSPKLPKKLPKFLNVDEINALLAMQLESKKYAVRDKAILELLYSCGLRVSELVN
                     LNQDQMDLIEGLVRIHGKGNKERIVPIGDKAVRALQAYLSERSSIQLIRDDDALFLNK
                     NGTRLTVRSIQRLVAHMTQEMGLSKTATPHMIRHSFATHMLGSGADLRSIQELLGHKS
                     LSTTQRYTHVGVEELSKVYDKTHPKS"
     gene            3693..4718
                     /locus_tag="VJC18_11455"
     CDS             3693..4718
                     /locus_tag="VJC18_11455"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HLD46040.1"
                     /translation="MCPRLFILTAVILCLCACASSTKDSSKTGVDNTGTDQDTAQTGD
                     VSGDDPAASDSDSETVPDSGAEIDTGSDSGTGTDSSPSEDTDDEVLPPACDLLVSTAF
                     LATASNIGAGLETLFEPSGIVWHERLNQLFLVSDSGILTRMNLDGSDVTNWSLPGDLE
                     AVTVTDPASDFVYVGVEHVAAIKEVNINTGLVTRTFTLPSGIYSETNQGLEALTFVPD
                     SSSVEGGYFWVGIQQTGMIHVYEVPLLSTTGDITITDSQQIVAWAPLPGVTDISDLYY
                     DGVHQKIFALYDSSNKLVSLTPDRILESQYDLPLSTHDQEGFAVDGECHLYIAEDPAG
                     THDVWRY"
     gene            complement(4945..7011)
                     /locus_tag="VJC18_11460"
     CDS             complement(4945..7011)
                     /locus_tag="VJC18_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46041.1"
                     /translation="MSSILSSSGLIGHGSLSRPIHLEGNGQPNPIPSHGPLPTGAEIQ
                     GYFASRTINVDGNTGTPRPTHEIGHPVQLQACRNVLIRSHPLERFIHPRGDIASVTSD
                     VTLSNILASTSNTAIGFGYPEGLRGVIQFYLGSAFLIGTDGQFKRLNGVDAHSRLAYA
                     MAAYVVRTDHDLIKYDPECLSGGQLSGKNLDDLIEWIRTLKEFDPQSACAMTLTLNVQ
                     WAQFAIPKPPVSVGNDFRSLQKLITDNTKPQLLISVIYSPTEVDSPLGHKGFHFVGVK
                     TTVNAGSPTDGGVLINCKINNVTRLQIAKIDEHVCLTDGACSLPAPQQRPTPSPTQVP
                     TRVTTSTKVPAPIRVAPRPQPTIPQKPAPAKQQPVVVARTAPTTADPLLELIPQPDQT
                     LMASNVWMHTETAHVLAQQLAEWLSGKTILRPVQLADVGSGAVPITLCRILAELATAK
                     DPSFSRLLFHLAILDSDQQAINRFCDLATTPAPGKDKWGKKSADIPSSPFPKSIVEKR
                     MLRGNTWSPTDVGRLRPEFLPQPVDVLFTGMLLHQGKPEQVYTLAQNVHSILTDGGLW
                     LIHDLFMPDTEPYLTIASEVTREYSGFSFPFKRVFRHAVEDNQARNAFLRALSSYKGA
                     SPSPNDLREQVTSQVFPYTMPMVTHLLQQAGFDTTVIPYAPLFGEHALAPWIGAIVAR
                     KKKPTY"
     gene            complement(7181..8626)
                     /locus_tag="VJC18_11465"
     CDS             complement(7181..8626)
                     /locus_tag="VJC18_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HLD46042.1"
                     /translation="MNFEHIIKSFNDVLWGTPLICMCLGAGIYFSLKTRFLQIRLIKD
                     MLKLLLEGKSSAQGISSFQALAVSLSGRVGTGNIAGVATAIALGGPGAVFWMWLIAFL
                     GAATAFVESSLAQIYKVEKDGQYRGGPAYYIEKGLKLKWYAVAFAVASIFSCGFFLPG
                     IQANSIAVSLQHAMGIPKTVSGAFVVMLLSAIVFGGIRRIGRVAEYVVPFMAIGYVLC
                     SLIIVAFNLEKLPAVLSLILSSAFGAHQVFGGIVGSAISFGVKRGIFSNEAGQGSAAH
                     AAGAAEVTHPAKQGLVQAFSVYIDTLFVCSATAFMILITGQYNVEGIVNNIPGVEIGP
                     MYTQKAVETVFGVGPIFVAISLFFFAFTTLMAYYYIAETNVAYLKKNPSQKWLITALR
                     IILLASTFYGSIKTADLVWAMGDIGVALMTWLNLVAIFMLRKPAFLCLQDYERQKKLK
                     INPVFRADDLGIANTDFWTKQPPRKTIDQAA"
     gene            9157..9627
                     /locus_tag="VJC18_11470"
     CDS             9157..9627
                     /locus_tag="VJC18_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46043.1"
                     /translation="MDRFKSPTMETDDDLMSVIIYNDLNPKRTTNNIHPKNHKWSSYH
                     HYAFGQEDPLLTDPEFYIGLGNTAHDRQNKYRLMIEEIIANDHRTPICPYRKDDGYIC
                     FIGNPLWVQTCHERLMKKVRLRRKEWQDRHRQFFELGKNKIGNNVVTVSATVSI"
     gene            9727..9873
                     /locus_tag="VJC18_11475"
     CDS             9727..9873
                     /locus_tag="VJC18_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46044.1"
                     /translation="MLVIINCLSKEQYYRYYYYYYRYYRYDGVPSVHDVPSVFVIRFK
                     TGTA"
     gene            10212..10877
                     /locus_tag="VJC18_11480"
     CDS             10212..10877
                     /locus_tag="VJC18_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46045.1"
                     /translation="MKNNIIFFVLLFCVCLLIGSSVWASLSPHIKQFSTIKQNEPSYA
                     DILFAYFKDKGITTSSLERLKKRVKAAPYFPALYVGYDHSLTEREGLKVSDNISLSGG
                     DVTIGPEDNDYNYYGYLGRTVHARAVWKLDEIIFNRNHLLIESERRELLKLQKTIGDD
                     LYKIYEDRYLYLIRYLQAKGHLQSKAMYYAKYLSLTDRIDAITGGQFHDRWWREQKQI
                     PGL"
     gene            10917..12029
                     /locus_tag="VJC18_11485"
     CDS             10917..12029
                     /locus_tag="VJC18_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46046.1"
                     /translation="MKIICSVLIVCLVVFVSCGLQRREHDLASEKQFYILSVSPTEGS
                     KITGETTISLNFSEPVWETTIARDSLFVITKSQYEVYTNQDWNDLYEAVTDGDVQIIE
                     SQMTIADDLRSVHLAINFDQIISDEYVVVALPKIQSQTYYPLNQAGLLSGSGLFSAHY
                     LVVVNDDASLSENEVDPDANAPTSYPDVPTTYPESDDTEEESDQITETFDWNRVLITE
                     VVTDPQQDHNDSEGGNGVLFDDEPGSGTVGSTDEYIEIFNGTEESLDVSKWSLNMIDG
                     TDVLQTLDSTLWDTHSSLDDIPLEFLPGELVVFGNPDGAINNTLTLELLDEAGNVVDA
                     VDIEDANADSTENEAYLRETGGTWVQGEASPGYFLE"
     gene            complement(12014..12343)
                     /gene="rplX"
                     /locus_tag="VJC18_11490"
     CDS             complement(12014..12343)
                     /gene="rplX"
                     /locus_tag="VJC18_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HLD46047.1"
                     /translation="MERLRKDDIVEVISGVSRGKQGKLLRFNDDRTRAYIEKVNLVKK
                     HSKASQKNPTGGIIEIESSIHVSNLMVVDPTKKIPSKVGIKTLKDGSKVRFLKKTGTE
                     LSAHSKK"
     gene            complement(12352..12636)
                     /gene="rpmB"
                     /locus_tag="VJC18_11495"
     CDS             complement(12352..12636)
                     /gene="rpmB"
                     /locus_tag="VJC18_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HLD46048.1"
                     /translation="MSNVCEVTGVRRYKVNRVSHANNKTKHYQQPNIQERRFLIPELG
                     QRVKIRISSRGLRTVDKHGGLSRYLVKANPEKLSANLQRVRKILISKGLH"
     gene            complement(12678..12935)
                     /locus_tag="VJC18_11500"
     CDS             complement(12678..12935)
                     /locus_tag="VJC18_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif domain-containing protein"
                     /protein_id="HLD46049.1"
                     /translation="MKLGIKIGDVVEVIKGKFSGQKSKIVSIDKKNDRVVLENLKKQK
                     TKTKSLHGSFHVLSLKLVKAEQPKTEEPKAEEKKEAVVAAS"
     gene            13171..>13828
                     /locus_tag="VJC18_11505"
     CDS             13171..>13828
                     /locus_tag="VJC18_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46050.1"
                     /translation="MNQTINQYTCQPTDENYIQTNPVSQNTDPNPRSVISAAVDEESK
                     AKVDQALIDPEFEKQLVSQLEETNLQAEAKNKDSFIADELEQLGEKDSMGVSLKAKVT
                     MGVKTVEGQVSCQVVKTNDGYEMTIAGKVGLGLSAELAKGKGRFKVQMGASVSVAGEK
                     AITLTFPNAQEVDKAIKLAGKVAITQAAIAVTATMPPVSYLIATKGSRAPVIGKYLVD
                     V"
BASE COUNT         3466 a         2969 c         3492 g         3901 t
ORIGIN      
        1 tttgatggtg acaacaggga cataccgctt ggttgcttcg atcttgcggg cgatgtaggc
       61 ttgcacatcg tattcctgcg ccttgatttc ctcgttgttt ctgacggcca tttcaatgca
      121 atcgctcaaa ttcagacggt attggggggg ggcggctagg gcccgtgatg ggagaaataa
      181 taaaaacgag atgatgagca gtgaaaatct tttcatggac atggcaatca ttgagctcgg
      241 cgattctgtc acagaattgg ccctggtgtc aaaaggggtt ttgcactggc atgttattta
      301 caatatgtat ttactcaggt cctggttatc gataattttt accaaacatt tctgcacata
      361 agggcttgtg atttcaaatg tcgttcccgc acgttcattg gcatcgtagg aaacttcttc
      421 catgaccttt tcgaggatgg tgtgtaatct tcgagcgcca atgttttcca tttgctcatt
      481 ggccttggcg gcaaaactgg cgatttcttc caacgcatcg tccgtaaatt gcagagtgac
      541 attttctgat cccatgagtg ccttgtattg cttggtgaga gaattctggg gctcggtgag
      601 gatacgcaga aaatcatgtt tggtgaggga attgagctcc accctgatgg ggaaacgtcc
      661 ctgaagttcg gggatgagat cgcttggttt ggagacatga aacgcgccag aggcaatgaa
      721 taaaatgtga tctgttttga cgaaaccgta cttggtggag actgtggagc cttcaacgat
      781 gggcaggata tcccgctgaa caccttcacg gctcacatcg ggacctcctc gaagatcaga
      841 tcgtgaggcg atcttatcga tttcgtcaat gaagatgatc ccgttatttt caacgaacga
      901 aacggccatt ttggtgactg tttccatgtc gatgagtttg gccgcttctt catgggtcag
      961 gatctcgagg gcttcgggaa cgggcacttt tttgcgtttc tttccctgtt ggaacatgcc
     1021 tccgaacatg tcgcgcaggt tactggagat gtcatcgaga gaaccgccgg ccgagatgat
     1081 ttcgaccatg gggactcccg ggcctccggt gaaggcaggg ggtttggggc ctatgtcgag
     1141 atcgaccttg cggtcgttga gtttgccatc tcgcaacatc tgacgcaact tttcgcgggt
     1201 tctcgatgtg tcttcttctt cgggaaccgt ggtctcttca attctcaccg gagtcatagt
     1261 catgtctacg ggaggtgctt ttttcttttt gatggggagc aggagatcca acagacgctc
     1321 ttcagccaaa tctttggctt tgcctttgac gcattcttgt tcgcgttggc gaatcatgtt
     1381 gacgccaatt tcggtgaggt cacggatcat ggattccaca tcttttccca cgtaaccgac
     1441 ctctgtgaat ttggaggctt ccaccttgta aaacggtgat tccgccagct tggcgagtct
     1501 gcgggcaatc tcggtttttc ccacaccggt agggccgatc atgatgatgt ttttggggat
     1561 gatttcatcc tgcatgtcgc ctttgacatt gagtctgcgc cagcggtttc tcaaagcgat
     1621 ggcgacgctt cttttggcct catgttggcc gatgatgtaa cggtcaagtt cctgaacaat
     1681 ttgtttgggt gtgtaatttt ttatttcgtt catgctgtac gcgtggatcg ctgatcgtgg
     1741 atcgtgaatc gatttttgac aggacattga caatctgtgc gtgattcacg agacacaact
     1801 gacaattcac tcttcaatgc tctccagggt gatgttgtgg tttgtataga tacaaatatc
     1861 gccggcgatt ttcatggact cttcggcgat ctgcttgacg cttaactttg tgtgcgtcat
     1921 caaggctcgg gccgcggcca gggcaaacat gccccctgag ccaatggccg caatgccatc
     1981 gtctggttcg atcacatctc catttcctga gatggtgaat aaactttctt tgtctgccac
     2041 aatgagcagg gcttcaagtc gtctgaggac tttgtctgtt cgccagtctt tggccaactc
     2101 gactgcggca cgtttgagat tgccggaata ttccttgagt ttggcttcaa atctttcgaa
     2161 caaggtgaag gcatcggctg tggagcctgc gaacccggcg atgactttgt cttcgttcat
     2221 gcggcgcact tttttggcct tgcctttgag gatagtattg cccagactaa cttggccgtc
     2281 acctgccatg gcgaccgaat tccctttttt gatggcaatg atggtggttg cgtgtgtttc
     2341 gtacatattt cgtggatcgt ggatcgtcga tcgttgatcg ttgatcgttg atcgttgatc
     2401 gtggatcgtg gatcgtggat cgtggatcga taaattatac gatccacgat tgacgattaa
     2461 cgattaactt ttgggatgcg tcttatcgta cacttttgac aactcttcaa cccctacgtg
     2521 tgtgtatctt tgggtggtgg agaggctttt gtgacccaac agttcctgaa tggatctcaa
     2581 gtccgctcct gagcccagca tgtgtgtggc aaagctgtga cggatcatgt ggggtgtggc
     2641 cgtttttgaa agacccattt cctgggtcat gtgggccacc aggcgttgta tggagcgtac
     2701 cgtcaaacga gttccatttt tgtttaaaaa caaggcgtcg tcatcgcgga tcaactggat
     2761 ggaactgcgc tcagaaagat aggcttggag cgccctgacg gctttatctc cgatggggac
     2821 gatgcgttct ttattccctt tgccgtgaat tcgtaccaga ccttcgatca aatccatttg
     2881 atcttgattg agattgacaa gctcgctcac acgcagaccg catgaataaa ggagttccaa
     2941 aatggccttg tcgcggacgg cgtatttctt gctttccaat tgcatggcca ggagagcatt
     3001 gatttcgtcg acgttgagaa acttcgggag tttttttggc agttttggtg aattgatgac
     3061 cttggctggg ttctggtcga tggttccttt tttgacaaag tatttaaaaa gggatcgcag
     3121 ggccgacagt ttgcgggcga tcgaggaggc accatttttt tgaaacagga gagagagata
     3181 tgacctgatc atcaatgggt tgatgcggtc aagcaacatg gcgttgtcgc tgatgacttc
     3241 tgcctggtga tttagcaggt aatggtaaaa ttcagtcaaa tcgcgccggt aactgagaat
     3301 tgtattttcc gcggcgttct tttggttcat caggtacgtc ataaattctt cgatgttttt
     3361 ttcgacagtc ataggaaaat ctcctgttga taagggtagt tgaaattatg cgaacttaag
     3421 ccaaattgca aggttttttt atatgacgaa ggtcacggaa ttctgtcgga cacattccac
     3481 ggtgctgtgc ccctaaccaa aagatgcgcc gatggttgac gggctgtttc aaggtcggtt
     3541 gtcaaggggg tagctcggtg cagatgcgct atgggctcaa tctggctgac ccttgtgcta
     3601 agacatcata gtattcttga gtttatgaca aagaactcat cgaaatatgt tgagattttg
     3661 tgtctgcgtt ctgtagaaag gtaaccatga caatgtgtcc acgcctattc attttgactg
     3721 ctgtgatctt gtgtctttgc gcctgtgcca gttcaacaaa ggattcctcc aagactggtg
     3781 tcgacaatac ggggacggat caggatacgg ctcaaaccgg tgatgtttca ggggacgatc
     3841 ctgccgcatc cgattctgat tcagaaactg tccctgattc tggcgctgag attgatactg
     3901 gatctgattc cggcactggt accgatagta gtcccagcga ggataccgac gatgaagttc
     3961 ttccacctgc ctgcgatctt ttagtttcca ccgcattttt ggcaacagcg tcgaacattg
     4021 gtgccggttt ggagaccttg tttgagccca gcgggattgt gtggcacgag cgtttgaatc
     4081 agttgttttt ggtgagcgat tccggaattc ttacgcgcat gaatctcgat ggctcagatg
     4141 tgactaactg gagccttcct ggtgaccttg aagccgtgac ggtaactgac cccgcgagtg
     4201 attttgtgta tgttggtgtt gaacatgtgg cggcaatcaa ggaagtcaat atcaatacag
     4261 gtttggtgac gcgcacattt actttgccgt ccgggattta ttcagaaact aatcaaggtc
     4321 tcgaagccct cacttttgtg cctgattcat cgtcggtcga aggcggttat ttttgggtgg
     4381 ggatacagca aaccgggatg attcacgtat acgaagtgcc acttttatcg acaactggtg
     4441 atattactat tactgacagc cagcaaattg tggcttgggc tcctctgccc ggtgtcaccg
     4501 atatttccga tttgtattat gacggagttc accaaaaaat ctttgccctc tacgattcgt
     4561 ccaacaagct ggtcagcctg acgcccgatc gtattttgga gtcgcaatat gatctgccct
     4621 tgtctacgca cgatcaggag gggtttgcgg ttgatggtga gtgtcacctc tacattgccg
     4681 aagacccggc gggcacgcat gatgtttggc ggtattgagt cgggtggtga aacttatgcc
     4741 gcgggcttgc ttgcactgat gcgttttctg aatctgaata acagcaaggt tgaatagagg
     4801 gcagatgcca agaacagcga ccgtatcagc gaccgtatga cggaggtcgt gtggcggctg
     4861 tatgacggag gtcgattgga gttgtgtaac agttcaacat atcaataagt tgtgttgttt
     4921 tgcaggacct tatttgggca ggtctcagta ggttggtttc ttttttcgag ccacgatggc
     4981 gcctatccaa ggggcaaggg catgttcgcc aaatagcgga gcatacggga tcaccgtagt
     5041 atcaaatcca gcctgttgta ataggtgagt caccataggc atggtgtagg gaaaaacctg
     5101 acttgtgact tgttcgcgca aatcgtttgg ggaaggggac gctcctttat aggaagatag
     5161 tgcgcgcagg aaggcattgc gtgcttgatt gtcctcaacg gcgtgacgaa atacgcgttt
     5221 aaacggaaac gaaaatccgg aatactcacg tgtgacttcg ctggctatag tcaggtaggg
     5281 ttctgtgtca ggcatgaaca aatcatgaat gagccacaga ccgccgtcgg tcagaatgct
     5341 atggacattt tgcgctagtg tatacacttg ttctggtttg ccttggtgca atagcatgcc
     5401 cgtaaataac acatcgactg gttgtggcag aaactcagga cggagacgcc ccacatctgt
     5461 gggggaccac gtattgccac gtagcatgcg tttttcgaca atcgattttg ggaacggcga
     5521 agagggaata tcagcagact ttttgcccca tttgtcttta ccgggggccg gagtggtggc
     5581 cagatcacaa aaacggttga ttgcttgttg atcgctatcg aggatggcca ggtgaaaaag
     5641 caaacgggag aagctgggat ccttggcggt tgccagttcc gctagaattc tgcagagggt
     5701 gataggaaca gcgcctgaac ccacatcggc caactgaaca gggcgtaaaa tggttttgcc
     5761 actgagccac tcagcaagtt gttgtgccag cacatgagct gtttcggtat gcatccaaac
     5821 attggaagcc atcagggtct gatcgggttg tgggatgagt tcgaggaggg gatcagctgt
     5881 tgttggtgct gtccttgcaa caacaacggg ttgttgtttt gctggtgcag gtttttgagg
     5941 aattgtgggt tgtgggcgcg gggcaacccg gattggcgca ggaacttttg ttgatgtggt
     6001 gacgcgcgtg gggacttgtg ttggtgatgg agttggtcgt tgttggggtg ctggtaagga
     6061 acaagcacca tcagtaagac aaacatgttc atcgatcttg gcgatttgca ggcgtgtgac
     6121 attattgatc ttacagttga tcagcacacc cccatcggtt ggagatcctg cgttgacagt
     6181 ggttttaacg cccacaaaat gaaatccttt atgacctaag ggggagtcaa cctctgttgg
     6241 tgagtatatg acagagatta aaagttgagg ttttgtgttg tctgtgatga gcttttgcaa
     6301 tgatctgaag tcatttccca cactcaccgg aggtttggga atcgcaaact gtgcccattg
     6361 cacgttcaag gtcagagtca ttgcgcaggc gctctgcgga tcaaactcct tgagtgtcct
     6421 gatccattca atcagatcgt ccagattttt tccactgagt tgtcctccgc tcaaacattc
     6481 agggtcatat tttattaaat catgatctgt tctgaccaca taggcggcca tggcgtaggc
     6541 tagacgcgag tgggcatcaa ccccgttgag tcttttgaac tgaccgtctg ttccaatcaa
     6601 aaaggcactg cccagataaa attgaatcac gcctctgagt ccttcggggt acccaaaacc
     6661 gatggcggtg ttcgacgtgg aagcaaggat gttcgacaaa gtaacgtcgc ttgtgacact
     6721 ggcgatgtca ccgcgaggat gaataaagcg ttcaagggga tgactcctga tcagcacgtt
     6781 tctgcatgct tgcagttgaa cgggatgacc aatctcgtgt gtgggccggg gcgtgcctgt
     6841 atttccatca acatttattg tcctactggc aaaatacccc tgaatctcgg cgcccgtggg
     6901 taagggacca tgtgagggga tggggttggg ctgtccatta ccttctaagt gaatggggcg
     6961 cgacaaagaa ccgtgtccaa tgagtcctga tgatgacaag attgacgaca taaagtgatc
     7021 tggttattac ctctaaccga tcaagatatc gaaagatttc tgtaaaagtt tcgtgttttt
     7081 tgatgattta cggatttttt atgtaactcc gtcatgagtt tttaatgagt ttttatagcg
     7141 ttgagctgtt ttgaaagatc aaaatgaata tagcgatgga ctaggctgct tgatcgatgg
     7201 tctttcgagg tggttgcttt gtccaaaagt cggtattagc aattcccaga tcatcggcgc
     7261 ggaagactgg attgattttg agtttctttt gtctttcata atcctgcaaa cataaaaacg
     7321 ccggttttct gagcatgaag atcgctacca aattcaacca cgtcattagt gccacaccaa
     7381 tatcgcccat tgcccaaacc agatcggccg ttttgatgga accatagaaa gtcgatgcaa
     7441 gcaggataat gcgcagggca gtgatcaacc atttttgaga ggggtttttt ttgagatagg
     7501 ccacgttggt ttcagcaatg tagtagtaag ccatcagtgt ggtgaaggcg aaaaagaaaa
     7561 gagagatggc cacaaaaatg ggcccaacac caaatacggt ttccacagcc ttttgtgtgt
     7621 acatgggacc gatttcaacg cctgggatgt tgttaacaat gccttcgaca ttgtattgcc
     7681 cggttatcag tatcatgaaa gcagtcgccg aacacacaaa gagggtgtcg atataaaccg
     7741 aaaaggcttg caccagtccc tgtttggcag ggtgtgtgac ttcggccgca cctgcggcat
     7801 gggccgcgct cccctgaccg gcttcatttg aaaaaatccc gcgtttcaca ccaaaactga
     7861 tggcgctccc cacaatgcct ccaaaaacct gatgagcacc aaaggcagac gacaagatca
     7921 gggacaacac agccggcagt ttttcgagat tgaatgccac aatgattagg gaacacaaaa
     7981 catagccaat agccatgaat ggaacaacat actcagccac tcggccgatg cgtctgatgc
     8041 ctccaaatac aatcgcactc agcaacatca caacaaacgc acccgacact gtctttggaa
     8101 tgcccatcgc gtgttgcaag ctcacagcaa tgctatttgc ctgaatgcct gggagaaaaa
     8161 aaccacagga aaaaatagag gccactgcaa acgccacagc ataccatttg agtttgaggc
     8221 ctttctcaat gtaatacgcc ggccctcctc gatattgacc atccttttct actttatata
     8281 tctgagccag agatgattcc acaaaggccg tggccgctcc taaaaaagct atgagccaca
     8341 tccagaagac agcgcccgga ccgccaagag cgatggctgt ggccacgccg gcgatattac
     8401 ccgtccctac gcgtccagac agagaaaccg ccagtgcttg aaaggatgaa attccctgcg
     8461 cggagctttt gccctcaagt aacaatttaa gcatgtcctt gatcagtcga atctgcagaa
     8521 atcgtgtttt gagtgaaaaa tatattcccg cgccaagaca catgcaaatg agtggggtgc
     8581 cccagagcac gtcattgaaa gatttaatga tatgttcaaa attcattcaa aaagcttagg
     8641 caacgacctt ttgaaaagca atatgaatat tatgacaaga ggacatcagg ggacatgaag
     8701 gggacatcat gacggagtcc cgcggacagt cccccttgtg ggattttatt ttgtgagaga
     8761 ctctattatt gttgagtgtt cttccaactt gattgatatt ctttaaatat tatttttttt
     8821 caaattggca cggttttggc aggtgtttag gacatggcaa attatcctag aagtttattg
     8881 gtctttgaag gatccgtttt tcatgtgaca tggcagtgtc ataacaagga ttggcttttg
     8941 aagtctgagt ttgcaaaggc gctttattat gaactactgc ttaaatataa aatggtgtac
     9001 gacattatta tcttttccta ttgtttgatg gataatcatg ttcacatgac aggccgttgt
     9061 gcttcgcaaa aattgttttc tgattttttt agggtcgtga atggttgctt ttcgagatcc
     9121 ataaacaaac acttttgtcg gagggggcag gtggtgatgg atcgattcaa atcgccaacc
     9181 atggaaaccg atgatgacct catgagtgtg atcatttata acgatctcaa tcccaaaagg
     9241 acaacgaata atattcatcc caaaaaccat aagtggtcat cgtatcatca ctatgctttt
     9301 ggtcaagagg atccactgtt gactgacccc gaattttata taggacttgg taacacagct
     9361 cacgacagac aaaataagta ccgattgatg atcgaagaaa tcattgcgaa tgatcaccgc
     9421 accccaatat gtccttacag aaaagatgat ggttacattt gttttattgg taatccattg
     9481 tgggtgcaaa cgtgtcatga acgacttatg aaaaaagtgc gtttgcggag aaaagaatgg
     9541 caagacagac accggcagtt ttttgaatta ggaaaaaaca aaattggcaa taatgtggtt
     9601 actgtcagcg caactgtttc catttgaagt gtgtgccgaa tccctccttc caaaatcttc
     9661 tgttattttt catttaataa tacgttaccg ttgagacgca tactatgacg gagtactgag
     9721 cctagcatgt tggtgatcat aaattgtctc tcaaaagaac aatattaccg ctattattac
     9781 tattattacc gctattaccg ctatgacgga gtaccgagtg ttcatgacgt accgagtgtc
     9841 ttcgtcatac gttttaaaac cggaacagca tgacggagct ccaactgtca atatcagtca
     9901 ataaatagtc tcaccatctt tcccaaaaat gggactaggc acagagaatg ggatgcgtac
     9961 acggaactcc gtcatacttg tgcttgtttt tttctcatgc attttttacc tatgcacaag
    10021 attgggtcgt gccatttttt gtctcaggat catccctgat ttgtcccaaa atgggacggt
    10081 gagacacttt ggaattccgt acagtgacct ccgtcatgcc gccagatcga acatgtttat
    10141 gatattgggc ttattttttt gtggagaatt attttttcaa agttggcacg tgcgttgcaa
    10201 aagacgttgt catgaaaaac aatatcattt tttttgtgct tttgttttgt gtttgtttgc
    10261 tcattggctc aagcgtttgg gcaagtctgt cacctcacat caaacaattt tccacgataa
    10321 aacaaaatga acccagctat gcagacattc tctttgccta cttcaaagac aaaggaataa
    10381 ccacttcctc cctggaacgc ttgaaaaaac gcgtcaaggc ggcgccctat tttcccgccc
    10441 tgtatgtcgg ctacgatcac tcgctcactg aacgagaggg actgaaggtg agcgacaata
    10501 tttctctgtc tggtggtgat gtgaccattg gccccgagga caatgattac aactattacg
    10561 gttatctggg ccgcacggtt cacgcgcgcg ctgtctggaa gctggatgaa attattttca
    10621 atcgcaatca tctgttgatc gagagcgaac gccgcgaact tttgaaactg cagaaaacca
    10681 tcggtgatga tttgtacaaa atctatgagg atcgttattt atacctgatc cgctatttgc
    10741 aggccaaagg ccacttgcag agcaaggcca tgtattatgc aaaatacctg agcctcaccg
    10801 atagaatcga tgccattacg gggggacagt ttcatgacag atggtggcgg gaacaaaaac
    10861 agattcctgg tttgtaactg ggaattgtct cacagccaca taaagaggag gtttgtatga
    10921 aaatcatttg cagtgtattg attgtttgtc ttgttgtttt tgtttcttgc gggctacaaa
    10981 ggagggagca tgacctggct tcggaaaaac aattttacat tttgagcgtg tctccaactg
    11041 agggttctaa aataacggga gaaaccacaa tatcgctcaa tttctcggag cccgtatggg
    11101 aaacaacgat cgcccgagat agtctttttg tcatcacaaa aagtcagtat gaagtttaca
    11161 caaatcagga ttggaatgac ttatatgagg cggtgacgga cggtgatgtg cagatcatcg
    11221 agagccaaat gacgattgcg gatgatttga ggagtgttca cctggcaatc aattttgatc
    11281 agattatatc tgatgaatac gttgttgtcg ctttgccaaa aattcaatcc caaacttatt
    11341 atccgttaaa ccaggcgggc ctgttatcgg ggagcggtct tttttctgct cattatctgg
    11401 tggtcgtgaa tgatgacgca tcactgtctg aaaatgaagt tgatcctgac gcgaatgccc
    11461 cgacttctta tccggatgtg cccacaactt atcctgagtc tgacgataca gaagaagaat
    11521 cagatcagat cacagaaacg tttgattgga acagagtcct cattaccgaa gtggtgactg
    11581 acccgcaaca ggaccacaat gactcagagg gaggtaacgg ggttctcttt gacgatgaac
    11641 ctggctcagg cactgtgggc agtacggatg aatacataga gatattcaat ggaacagagg
    11701 aaagtctgga tgtgagcaag tggtccctga acatgattga tggaacagat gtgttacaga
    11761 ctttggatag tacattgtgg gatactcatt cgagtctgga tgacattcct ttggaatttt
    11821 tacccggcga gcttgttgtt tttggcaatc ccgatggagc gattaacaac acattgacct
    11881 tggaactgct tgatgaagcg ggaaatgttg tggacgcggt cgacattgag gacgccaacg
    11941 cagacagcac cgaaaatgaa gcgtatcttc gagaaaccgg gggaacgtgg gtacagggag
    12001 aagcgagccc aggctatttt ttagaatgag cactcagctc ggttccggtc ttttttagaa
    12061 agcggacctt tgaaccatcc ttgagggtct tgatgccgac cttgctgggt atttttttgg
    12121 tggggtcaac gaccatcaga ttcgaaacat gaatcgagga ttcaatttcg atgataccac
    12181 cagtcgggtt tttctgacta gcctttgaat gctttttgac cagattcact ttttcgatat
    12241 aagcgcgggt gcggtcatcg ttaaagcgta aaagtttgcc ttgtttgcct cggctcacgc
    12301 cggaaatcac ttcgacaatg tcatcttttc ttaatcgttc cataataaac ctcaatgtaa
    12361 gcccttgctg ataagaatct ttctaacacg ttgcaaattt gctgaaagtt tttctgggtt
    12421 tgctttgact aaataacgcg acaagccacc atgtttgtcg actgttcgca aaccgcgtga
    12481 gctgattctg attttgaccc tttggcccaa ttcaggaatc aaaaaacgcc tctcctgaat
    12541 gttgggttgt tgatagtgtt ttgttttatt gttggcgtgt gataccctgt tgaccttgta
    12601 acgccgaaca ccagtgactt cacatacatt tgacataacg aactccttgt ttcaccacat
    12661 aaatttatca gccgttttta tgatgccgct acgactgctt cttttttctc ttctgctttg
    12721 ggttcttccg tcttgggctg ctcagccttt accagcttca ggctcagaac atgaaagctt
    12781 ccatgtaaag attttgtttt ggttttttgt tttttcaagt tctcaagaac gacgcgatca
    12841 ttttttttat cgatggaaac gatcttggat ttttgaccgg aaaattttcc tttgatgacc
    12901 tcgacaacgt ccccaatttt gattcccaac ttcataaatt cacctgcaat ataagggtta
    12961 aaaagcgagg cgtgtcacta gcaataataa gattcagagg caagaaaaat gtgaaacacc
    13021 cgtctcagca cctgtctcag gcaagaaata atgggactga ggatttgtga atgtgttttg
    13081 taactttatg atatttaaga ataataataa aaatgaattc tggcatggtt tgtgctgagg
    13141 atagagcaga taacctttac aggagatttt atgaatcaaa caatcaatca atacacatgt
    13201 cagcccacag atgaaaatta tatccagaca aaccccgttt cccaaaatac cgatcccaat
    13261 cccaggtctg tcatttcggc cgcggttgac gaagaatcaa aggccaaggt cgaccaggcc
    13321 ttgatcgacc ctgaattcga aaagcagttg gtgagtcaat tggaggaaac caatttacag
    13381 gccgaggcaa aaaataagga ctctttcatt gcagatgaac ttgaacagtt aggcgaaaaa
    13441 gattcaatgg gggtcagtct caaggccaaa gtgacgatgg gagtcaagac tgtggaggga
    13501 caggtttcgt gtcaggttgt gaaaaccaac gatggatacg aaatgaccat tgcaggcaag
    13561 gtggggcttg gcctttccgc ggagcttgca aagggaaagg gtcgttttaa ggtacagatg
    13621 ggtgcctctg tctcggttgc tggcgaaaag gccatcacat tgacattccc caacgcccag
    13681 gaagtcgaca aggccatcaa gctcgcgggt aaggttgcca tcacacaagc ggcgattgcc
    13741 gtgactgcca cgatgccacc cgtgtcgtac cttatcgcca ccaaggggtc gcgagcgcct
    13801 gttattggca agtatctggt tgatgtta
//
LOCUS       DATDUN010000826         1029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_577054, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000826 DATDUN010000000
VERSION     DATDUN010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577054"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>1029
                     /locus_tag="VJC18_11510"
     CDS             21..>1029
                     /locus_tag="VJC18_11510"
                     /inference="COORDINATES: protein motif:HMM:NF018208.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46051.1"
                     /translation="MKEYDAQTTATQNEIAQDETWVEERRLTLAEVESALNELQQKVY
                     EWENHLRLAEAKLQGRRDEDERIGLDCEQFDRQVEELNENLAGNQNGLSQINEKLCLA
                     ELECESFDQSVAEQQQYLEALEESSQQLFLILEQSRNDYHQSSQQLVELATRREGLEH
                     RTQELRLKQENDQNDLDELSGRYKKLEALLHDSQSGLSSVKQLKLSLGEKTQELFQSL
                     SDEELQIQTEQSQLDDTKQDLLQKKSRLQSLRELEKNFEGYHAGPRQILKRKQTGEMS
                     SVMGSVAEFIDVANQFESAVSAVLGEVMQSVVVTTQHESITCADYLKSESLGRSSFVA
                     LS"
BASE COUNT          309 a          214 c          290 g          216 t
ORIGIN      
        1 aaaacgaatg tcttgtcaaa atgaaagaat acgatgcgca gacgactgcc acccaaaatg
       61 agatagcaca ggatgaaacc tgggttgaag aaaggcgatt gaccctggcc gaggttgaat
      121 ctgctctcaa tgaattgcaa caaaaggtat acgaatggga gaatcacctt agattggccg
      181 aggccaaact gcagggacgc agggatgaag atgaaaggat aggtctggat tgtgagcagt
      241 ttgatcggca agtggaagaa ctcaacgaga atcttgcggg caaccagaac gggttgtcac
      301 agatcaacga aaaactttgt ctggccgagt tggaatgtga gtccttcgat cagtctgtgg
      361 cggagcaaca acagtacctc gaagcgctcg aagaatccag tcaacagttg tttctgatct
      421 tggaacagag tcgtaacgat taccatcagt cgtcgcaaca gttggtcgaa ctcgcgacga
      481 gacgcgaagg gctggagcac cgtactcagg aactgcggct caaacaggag aatgatcaga
      541 acgatttgga cgagctctcg ggcagataca aaaaactgga agccctgttg catgattctc
      601 aatctggttt gtcatcggtc aaacaactca aattgtcttt gggagaaaaa acacaggagt
      661 tgtttcagag cttgagtgac gaagaattac aaatccaaac ggagcagagc caactcgatg
      721 acaccaaaca ggatcttttg cagaaaaaat cgcgtttgca atccctcaga gaactcgaga
      781 agaattttga ggggtaccac gcggggccgc gtcagatcct caaaagaaaa caaacgggag
      841 agatgagttc tgtgatgggt tcagtggcag aattcatcga tgtggccaac caatttgaga
      901 gcgccgtgtc ggctgtgttg ggcgaggtga tgcaaagcgt ggtggtgacc actcagcacg
      961 agagcatcac ctgtgcggat tacctcaaga gtgagagctt gggacgcagt tcgtttgtgg
     1021 cgctttctt
//
LOCUS       DATDUN010000827         1730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_577868, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000827 DATDUN010000000
VERSION     DATDUN010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577868"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VJC18_11515"
     CDS             <1..1546
                     /locus_tag="VJC18_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLD46052.1"
                     /translation="LPEGMVLAGTTDEDIAKMKTSIKTDKIYQGLVKQIDTKSNTMQV
                     QVGAHTGSIIARDFGWARKRNNAASGYDDVMYVKSPQSVAKEGDVIEVKVVDIDRLTD
                     KQKAAYKTDALYFSLEETPKVEGALFCYDPMTGYVKAIFGGKDFANSEFNRATQALRQ
                     TGSVFKPLLYAAAIDKGYRPDTIINDEPLKIPDGPGRFWTPQNYGGGYRGPMTLHSAL
                     VASRNVVSVRILLDVGVDYVTAQLRKLGVTTKIDKVYSMALGANDMKLSEVTRAFGIF
                     PTGGILPEYIFIKKMTDRYGNIVEQKQPAKIVPYAEQVEQGDLIKQTAVPNPTVRDED
                     ALRKDLWTVAQHAVKRDQLMLTPSEEILLYGKHLPEGYVMNPKTMATMVGIMQDIVNF
                     GTGYRVHELGRPVAGKTGTTNDLTDCWFVGYTPNLVAGVWTGYDQAINKVGGGETGGK
                     AAAPIFLYFMKDYLKDTPVAQFPNPQDVQLSALEPAIDVMPGNVSDLFTDTNASGGAD
                     FFADDI"
BASE COUNT          463 a          458 c          429 g          380 t
ORIGIN      
        1 cctgcccgaa ggcatggttc tggcaggcac aaccgacgaa gacatcgcca agatgaagac
       61 cagcatcaag acagataaaa tctatcaagg cttggtcaaa cagatcgaca caaaaagcaa
      121 taccatgcaa gtgcaggtcg gggctcatac aggctcgatc attgccaggg attttgggtg
      181 ggctcgaaag cgcaacaacg cggcctcggg ttatgacgac gtgatgtacg taaagtctcc
      241 gcaatctgtt gccaaggaag gtgacgtcat cgaagtcaaa gtggtggaca ttgaccgatt
      301 gaccgacaaa caaaaagccg cctacaagac tgatgccctc tatttttcgc tggaagaaac
      361 acctaaggtc gaaggcgccc tgttttgcta cgatcccatg acgggctatg tgaaggccat
      421 ctttggcggc aaggactttg ccaacagtga attcaatcgc gccacccagg ccttgcgaca
      481 gaccggttcg gtattcaaac cacttctgta cgcggccgcc atcgacaaag gttatcgtcc
      541 cgatacgatc atcaatgatg aaccgttgaa aattcccgat ggtcctggcc gcttttggac
      601 acctcaaaac tacgggggcg gttatcgtgg ccccatgacc ctgcacagtg ccctggtggc
      661 ctcccgtaac gtggtttccg tgcgcatttt actggatgtc ggagtggatt acgtaacggc
      721 ccaactccgt aaattgggtg tcaccacaaa aatcgataaa gtttattcca tggctctcgg
      781 ggccaacgac atgaagctca gtgaagtcac ccgcgccttc ggtatctttc ccaccggtgg
      841 catattacct gaatacatct ttatcaaaaa aatgaccgat cgttacggaa acatcgttga
      901 gcaaaagcaa cctgccaaaa ttgtccctta cgctgagcag gttgaacaag gcgatttgat
      961 caaacaaacc gcggttccca accccacagt acgggacgaa gacgccctgc gaaaggatct
     1021 gtggacagtg gctcaacacg ccgtcaaaag agatcaactg atgctcacac cttcggagga
     1081 aatcctgttg tatggcaaac atctccccga aggctatgtc atgaatccca agaccatggc
     1141 caccatggtt ggcatcatgc aggacattgt caattttgga acaggttacc gcgtgcatga
     1201 actggggcgt cctgtagccg gcaaaacggg taccaccaac gacctgacgg attgttggtt
     1261 tgtggggtac acccccaatc tcgtggcggg ggtctggaca ggctacgatc aggccatcaa
     1321 caaggtcggc ggcggggaaa ccggtgggaa ggccgcggcc ccgatttttc tgtacttcat
     1381 gaaggattat ctcaaagaca cacctgtggc acagttcccc aacccccagg atgtgcaatt
     1441 gtcagccctg gaaccagcca ttgacgtgat gcccggaaac gtgagtgatt tgtttaccga
     1501 taccaacgcc agtgggggtg ccgatttttt tgcggacgat atatgaaaaa cattatttta
     1561 ttcatgctgt ttttactaac cctatcaacc ccaaccttcg gaggcgacat gactgcattc
     1621 aaacctgttg aacccaccac ccccatgact tttgaggctg gtaaaaaata ttttgccaca
     1681 atcaagacgg acaaaggaga tatttactgt gaactgaatc cccttcaagc
//
LOCUS       DATDUN010000828         1896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_578051, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000828 DATDUN010000000
VERSION     DATDUN010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578051"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /gene="yajC"
                     /locus_tag="VJC18_11520"
     CDS             <1..362
                     /gene="yajC"
                     /locus_tag="VJC18_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HLD46053.1"
                     /translation="AYAQQAGAAAPTGGGTGPFVMMAVIFAIFYFLVIRPQQKQQKKL
                     KHMLSEAKRGDEIVTTGGMHGRIVDIVENNLVMIQVANNVIIKMERGAIQHIKGYDIK
                     ELEKKNEKDANKDKKVA"
     gene            511..>1896
                     /locus_tag="VJC18_11525"
     CDS             511..>1896
                     /locus_tag="VJC18_11525"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46054.1"
                     /translation="MLSIVFSIYFLIPTVIGLKDLRATFEKEGKALPWYYQLLPNQEM
                     NLGLDLRGGAYVEMEVSLEDALKQELNGYVNGINRAVLDDYNQDADKTENKNPVYQGV
                     LLQNNRIRVEYPADKKDEVLSGLVGYLGNQAFTFGNDRYELFLNIVSNAAQARKIAFE
                     ATKGLPDYQGDVLFVGQEKYLAVSFNSQEQKQKLIELFSLEQYQTDFTLADQTTVSVA
                     YLTPSDKFLAGKRKDIIDQAANSVRNRIDRFGVAEASVSRQSSDRLVVELPGVKDPEQ
                     VINIIKRTGKLEFRIVDSSLTQPDLEDLVQKKREELKLKNIYEDEALAQVNQALKGSL
                     PPHTEILFELKRDPVTKKISNAVPYLLEDKADVTGDMLDSTSVQTENNMPYVLMTFNN
                     TGTKNFGKLTTDNVGKQLAIVLDNVVTSSPVIRGPITAGRAQIELGIGPYNDLLKEAQ
                     DIVMILKEGALP"
BASE COUNT          534 a          443 c          498 g          421 t
ORIGIN      
        1 ccgcatacgc acaacaggct ggcgcagccg cgccgacagg tggcgggaca gggccgtttg
       61 tgatgatggc cgtcatcttt gccatttttt attttttggt catcaggccg caacagaagc
      121 aacaaaagaa actcaaacac atgctttctg aggccaagag aggcgacgaa atcgtcacca
      181 cgggtggcat gcacggccgc atcgtcgaca tcgtggagaa caatctcgtg atgatacagg
      241 tcgcaaacaa tgtcatcatc aaaatggaac gaggcgcgat tcaacacatc aagggttacg
      301 atatcaagga actcgaaaag aaaaacgaaa aggacgccaa taaagacaaa aaagtggcgt
      361 aacaggttac tggtaacggg taacgggttt tggtcaatga tcaatggtca atggtcaaca
      421 gtcctcacaa gggtgacttt ggactttggt ctttggtcct tggtctttta gtcaatgtct
      481 ggaactttaa agtttaaatt ttggatgttc atgttgagca tcgttttttc catctatttt
      541 ctgatcccca ccgtcattgg gctcaaggac ctcagggcaa cgttcgaaaa agaaggcaag
      601 gccctgccct ggtactatca acttcttccc aatcaggaaa tgaatctggg tctcgacttg
      661 aggggagggg cctatgtaga aatggaagtg agccttgaag acgcgctcaa acaagaactc
      721 aacgggtacg tgaacggcat taaccgcgcg gtgcttgatg attacaacca ggatgccgac
      781 aagaccgaaa acaaaaaccc ggtttatcaa ggtgtgttgc ttcaaaacaa tcgtatccgc
      841 gttgaatacc ccgccgacaa aaaagatgaa gtgttgagcg gtttggttgg ctatctgggc
      901 aatcaggcct tcacttttgg caatgatcgt tatgagttgt ttcttaatat cgtatcgaac
      961 gccgcgcagg cccgcaagat agcctttgag gccaccaagg ggcttccgga ttaccagggg
     1021 gatgttctct ttgttggtca ggaaaaatat ctggcagttt cttttaactc acaggaacaa
     1081 aaacagaaac tcatcgagct tttttcactg gagcaatatc aaaccgattt taccctggcc
     1141 gatcaaacga cagtttcggt cgcttacctg accccttcgg acaagtttct ggccggaaaa
     1201 agaaaagaca tcatcgatca agccgccaat tcggtgcgta accgcattga ccgctttggt
     1261 gtggccgagg ccagcgtgag caggcagagc agtgatcgcc tggttgttga actccccggc
     1321 gtgaaagacc cggaacaggt gatcaacatc atcaaacgga caggcaagct cgagtttcgc
     1381 attgtggact cgagtttgac gcaacccgat ctggaggatc tggttcaaaa gaaacgggaa
     1441 gagctaaaac tcaagaacat atacgaagac gaggctctcg cgcaggtcaa tcaagcactg
     1501 aaggggagtt tgccgcctca taccgagatt ttatttgagc tcaagcggga tccggtgacc
     1561 aaaaaaatca gcaacgcggt tccttattta ttggaggaca aagccgatgt gaccggcgac
     1621 atgctcgaca gcacgagcgt gcagacagaa aacaacatgc cctacgttct catgacgttt
     1681 aacaacacgg ggacgaagaa ttttggcaaa ctcacgaccg acaatgtagg gaaacagctg
     1741 gcgatcgtgt tggacaacgt ggtgaccagc tcgccagtca tcagggggcc gatcacggcg
     1801 ggacgggcgc agattgaatt gggcattggg ccgtacaacg atctgctcaa ggaggctcaa
     1861 gacattgtga tgatcctcaa agaaggtgcc ttgccc
//
LOCUS       DATDUN010000829         4648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_245942, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000829 DATDUN010000000
VERSION     DATDUN010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245942"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VJC18_11530"
     CDS             complement(<1..523)
                     /locus_tag="VJC18_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLD46055.1"
                     /translation="MKRELRFFDILCIGVNAIVGSGIFLIPGRLIGGMGPASILLYVV
                     CGLMLIPVGLCFAEASSRTDRSGGSYIYVEKAFGHGLGFATGWIDIATALFSYPAAAI
                     GLPKYLATFFPIVGQGIYPYVIAGIVIVLLAAINIRGIRPGATTVNIFTVSKLVPLLI
                     LIGVGAWFFKPSAF"
     gene            737..1192
                     /locus_tag="VJC18_11535"
     CDS             737..1192
                     /locus_tag="VJC18_11535"
                     /inference="COORDINATES: protein motif:HMM:NF020400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HLD46056.1"
                     /translation="MSKIAMISCGNVKNELNCSSFGCHQNFNARTGGFAPYQNEQVYE
                     LVGTLSCTGCPTLVAPEKILNKVKPLVAMGNVDAIHFASCMLAVCPFVNKYKSVIEEN
                     CPGVKVVLGTEDTGSPEETRNLLEVFKGVVKKLLTQNKPDLMGEFKKMM"
     gene            1627..2199
                     /locus_tag="VJC18_11540"
     CDS             1627..2199
                     /locus_tag="VJC18_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HLD46057.1"
                     /translation="MRLLPKTLTQRPFSLSEAACLGLNKMVIHRMLQQGVLDRVSRGV
                     YVMSGVDDGTNENLFKTATIRCGRPSAVCLLSALEYHHLTDEISHQVWILVPTEKRVR
                     AKELKLIRSRKPKWNLGIKKMDGYWITTVERTLIDCLLFKNLLGLQTALAALNRAVEE
                     NRIRMGDVFDLAKKMRVLDKLRPYLEARSV"
     gene            2196..3059
                     /locus_tag="VJC18_11545"
     CDS             2196..3059
                     /locus_tag="VJC18_11545"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLD46058.1"
                     /translation="MNTETVKKVLRGIRRIVEQNEKLSINEVRIIVALERAIARLIHD
                     RVLQKHLIFKGGFVLLKNYDSSRFTRDVDALALAISKEKISELVGEVLSVDLDDGLWF
                     GDLKVNELTEAGQYGAYRFGFAFQIGEPEQKKIHNLARLHIDIGFSDRVSGIHADSKM
                     IPILKGDPPLCWNIYPLEYIVAEKLHTLIQRGSANSRAKDIFDLVHLIPKCVDKKALV
                     AAVQNVFDHRKAPLPKSFAETIVGIDNVFLTQGWPSVKTLASKPSFEETWQSLITFLA
                     GVDGTMISYRS"
     gene            complement(3047..>4648)
                     /locus_tag="VJC18_11550"
     CDS             complement(3047..>4648)
                     /locus_tag="VJC18_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46059.1"
                     /translation="AQCCRSSADSTPMRDIMLRPIDRAGYHLSLPDDQNRPPEQNDSL
                     ANDIIDFCSDSPLTGSLKLILDGIGNHNGLADRADLKLFCAPLGLNPKEMTYSLLEQT
                     IINHYFNQYDADHFVGRTYPQAWSVLAPYVKTPTQETSEPKKYRASLRWLAAQLTAGP
                     DIFVLPQWIPVPNQSGSLLIFTFENKDRNLSAEANPPTHTKYILPGDGAMPSLYVTLA
                     LHEVHKNKQERIVVLDYFAGEASDLFAPDTPPLTGLEYEHLFADSLHSVIQSTLGRQE
                     HFSFITHSAGYYQNLFWLKFYGKTVDAEGLPYCDRLDAVDEMAGAWMTTTLQRLMTRV
                     SGKKNASYNSLNAGLIKILTSGDLETALKLKNDLLGTLTIGQHSLFENDFDREQGPYF
                     DDLIMDNETITGLIPSQVRYQYTVATNDRFIDWDKALVRATLDWGRREQQPVFTVLVD
                     PKHSPKHCQKQLINEFCKNDASAEFADASRPDQIIINRGRIDERKITVRWIEAPTNPG
                     GHFFMLHEEILNLITASAYIQGQDR"
BASE COUNT         1216 a          961 c         1186 g         1285 t
ORIGIN      
        1 cgaaggcgga gggtttaaaa aaccacgcac ccaccccaat gaggatcagc aagggaacca
       61 gtttgctgac tgtaaaaata ttgactgtgg tggcgccggg tcggatgccg cggatgttga
      121 tggccgccaa taatacaatc acaatcccag caatcacgta agggtaaatc ccttgtccca
      181 caatggggaa aaacgttgcc aaatatttgg gaaggccgat cgcggcggca gggtacgaaa
      241 aaagggctgt ggcaatgtcg atccaaccag ttgcaaaacc caaaccgtgg ccgaaggcct
      301 tttcgacata aatgtatgat cccccgctcc ggtctgtgcg tgatgaggct tcggcgaaac
      361 acagccccac ggggatgagc atcaagccgc acaccacata aagcaggatg gatgcgggtc
      421 ccatgccacc gatgagtctg ccgggaatca aaaaaatacc ggagcctacg atggcgttga
      481 caccaatgca caaaatatca aaaaagcgaa gttcacgttt catggatacg ctcccgttga
      541 tttggatctc acccaaaaaa aataccaaaa aaatgttttt cgactttttt atcccagcaa
      601 agatcggtca tattggcaag cactatctct tgaaaacagc aatgcgccgc agagccattg
      661 actgactggt taccgaaatc actgtttgtc ttggctgaga tcatatagta cacccaccgc
      721 acataaggag ttttttatgt ccaaaatcgc catgatcagt tgtgggaatg tgaaaaacga
      781 actcaattgt tcgtcattcg ggtgtcatca aaacttcaat gcgcgcacag gcggctttgc
      841 cccctatcaa aacgaacagg tttatgaatt ggtagggacc ctcagttgca cgggttgccc
      901 cacactggtg gctcccgaaa aaatattgaa caaggtcaaa cctcttgttg ccatgggaaa
      961 cgtcgatgcc attcattttg cctcttgcat gttggcggtc tgtccttttg tgaataaata
     1021 caaatcagtc attgaggaaa attgtccggg cgtaaaggtg gttttgggca cggaagacac
     1081 aggttctccc gaagagacta gaaatttatt ggaggtcttc aagggtgtgg tgaaaaaact
     1141 gctcacgcaa aacaaaccgg atttgatggg tgagttcaag aagatgatgt gaaatatttt
     1201 tttttacttt tcttccgggc atgtgacttt gatcgatgtt ttgtcgacca caacgaagtg
     1261 ctgggcaccg atttgagttg ttctttttcc gattccgaaa gcagctaaac tgggtttggt
     1321 gtcaggcacg caataccatg atgtttaacc aattgatcag cgatatctct atgttttggt
     1381 tttagggccg agcaggcatt tgtttcactc atggtagttc tccttgttgt gattctttct
     1441 tattgagata ttgtccgtct aaaccatttg ttgcgttaaa tcgcgtttgc atcgcgtttt
     1501 cagtagcacg cgtgatacag tgcaccgtgg tgggtgcacg tttcactgtg atacatgagc
     1561 ttatccacgc agagagcatg aattaccata ttgacacatt tagtagattt gtgacaatat
     1621 agtaacatgc ggttgttacc gaaaacgttg acacaaaggc cattttctct atcagaggca
     1681 gcttgtcttg gactgaacaa aatggtcata caccgtatgt tgcaacaagg tgtgttggat
     1741 agagttagtc gaggcgtgta tgtgatgagt ggcgttgatg atggcacgaa cgaaaatctt
     1801 tttaaaacag cgacaattcg ctgtggccgg ccctcagctg tttgtttgtt atcggctctg
     1861 gaatatcatc acttgactga tgaaatttct caccaggttt ggattttggt tccaaccgaa
     1921 aaacgggttc gggccaaaga attaaaactt attcgttcac gcaagcctaa atggaatttg
     1981 ggaataaaaa aaatggacgg ttactggatc accacagtgg aaagaactct catcgattgt
     2041 ttgttgttca aaaatcttct tggtctgcaa acggctcttg cggcacttaa tcgggccgtt
     2101 gaagaaaaca gaatcaggat gggtgatgtt tttgacttgg caaaaaaaat gcgtgttctt
     2161 gacaaattgc ggccatactt ggaggcaaga tcagtatgaa cacagagaca gtgaaaaagg
     2221 tcttgcgggg cattcgccgc attgttgaac aaaacgagaa attgagcatt aacgaagtgc
     2281 gtatcattgt tgctcttgag cgggcgatcg cacgattgat tcacgatcgc gttttacaaa
     2341 agcatctgat tttcaagggc ggatttgtgt tgttgaaaaa ctatgacagt tcgcgattta
     2401 cgcgtgacgt tgacgctctg gctcttgcca tttctaaaga gaaaatcagt gagttggttg
     2461 gtgaggtgtt gtcagttgat ttggatgatg gtctctggtt tggcgatctt aaggttaacg
     2521 aattgacaga agcaggccaa tatggagcat accggttcgg ttttgctttt cagattggcg
     2581 agcctgaaca aaaaaaaatt cataatctgg cgcggctgca cattgatatt ggcttcagtg
     2641 atcgcgtgtc ggggatacac gctgattcca aaatgattcc gattttgaag ggtgatcctc
     2701 cactgtgttg gaacatatac ccattggaat acatcgtggc agaaaagctt catactctta
     2761 ttcagagagg ttctgctaac tcacgggcaa aggacatctt cgatttggtt catttgatac
     2821 cgaagtgtgt tgataaaaaa gcactggtcg cggctgtgca gaatgttttt gatcatcgta
     2881 aagctccgtt accaaaatcg tttgctgaga ccattgtggg gattgacaat gtttttttga
     2941 ctcagggttg gccgagtgtc aagacgctcg cgagcaaacc ctcatttgaa gagacctggc
     3001 agagcctgat aacttttctt gctggtgttg atggaacaat gatttcttat cgatcttgac
     3061 cctggatgta tgcggatgct gtaatcagat tcaatatctc ttcatgcaac atgaaaaaat
     3121 gcccgccggg attggttggt gcttctatcc agcgtaccgt gattttgcgt tcatcgattc
     3181 taccacggtt gatgatgatt tgatctggtc gtgatgcgtc cgcaaattca gcagaagcat
     3241 catttttgca aaattcattg atgagttgtt tttggcaatg tttgggtgag tgttttggat
     3301 caaccaacac ggtaaaaacg ggttgttgct cccggcgacc ccaatccagt gtggctctga
     3361 caagtgcttt gtcccagtca atgaaacgat cgttggtggc cactgtgtat tgataacgga
     3421 cctgagaggg gatcaggccc gtgatggttt cgttatccat gatcagatcg tcgaaatagg
     3481 gtccttgttc gcgatcaaaa tcattttcga acagactgtg ttggccgatg gtcaaagttc
     3541 ccaaaagatc atttttgagc tttaaggctg tttccagatc gccgctggtg aggatcttga
     3601 tgagaccggc attgagtgag ttgtaggaag catttttctt gccagaaacc cgtgtcatca
     3661 gacgttgcag ggttgttgtc atccaggcgc cagccatttc gtcgacggca tcaagtctgt
     3721 cgcagtaagg caggccttca gcgtccactg ttttgccata gaatttcaac cagaacagat
     3781 tttgataata accggcgctg tgcgtaatga atgaaaaatg ttcctggcgt cccaaagtgc
     3841 tttgtatcac tgagtgcaaa gagtctgcaa aaagatgttc gtattcaaga cctgtcaggg
     3901 gcggtgtgtc aggggcaaac aaatcactgg cctcgccggc aaagtaatcc agcaccacaa
     3961 tgcgttcttg tttgtttttg tgcacctcat ggagggctaa agtcacgtac aatgagggca
     4021 tggccccgtc acccggcaag atgtattttg tgtgggtggg agggtttgct tctgctgaca
     4081 aattccgatc cttgttttca aaagtgaata tcagcaatga tccggactga ttgggcacag
     4141 gaatccattg tggcaataca aaaatatctg gaccggcggt gagttgtgcg gccagccaac
     4201 gcaaggaggc acgatacttt ttgggctcag atgtttcttg cgtcggtgtt tttacgtagg
     4261 gcgcaagcac agaccaggct tgcgggtacg tcctgcctac aaaatgatct gcatcatatt
     4321 gattgaagta atgattgatg atggtttgct ccaaaaggga gtaagtcatt tccttgggat
     4381 tgagtcccag cggggcacag aagagtttca gatcagccct gtccgcaagt ccgttgtggt
     4441 tgccgattcc gtcaaggatc aatttgagtg agcctgtcag tggggagtca gaacaaaaat
     4501 cgatgatgtc gtttgcaagt gaatcgtttt gttctggagg acgattttgg tcatcgggaa
     4561 gtgataaatg ataaccggcc cgatcgatcg gtctgagcat gatgtctctc ataggtgtgc
     4621 tgtcagcact actcctacag cactgggc
//
LOCUS       DATDUN010000830         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_411815, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000830 DATDUN010000000
VERSION     DATDUN010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411815"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VJC18_11555"
     CDS             complement(<1..1220)
                     /locus_tag="VJC18_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HLD46060.1"
                     /translation="MIQNVPQSIPPFEPGVRRAPDRGFTLNKNETKLALKNALRYIPK
                     KYHQTLGQEFLEELKTRGRIYGYRFRPRGNIIGKPIDQYEGKCLAGKALQVMIDNNLD
                     FRVALYPYELVTYGETGQVVQNWMQYQLINAYLKILTEEQTLVVASGHPLGLFASKPS
                     APRVINTNGLMVGLFDNQTDCHRAMQLGVANYGQMTAGGWMYIGPQGIVHGTFNTVLN
                     AGRQRFGLGPDEDLRGHLFVSSGLGGMSGAQPKAVEIAGGVALIAEVDASRINTRHDQ
                     GWVAKVTADRAEAFAMAQKAMAQKQPLSIAYHGNVVDLLEYAVTNTISIELLSDQTSC
                     HSVYDGGYCPQGITFEERTKYLAEDRDGFKKLVDQSLRKHFECVSTLVARGTYFFDYG
                     NSFMKAVFDAGVRE"
     gene            complement(1217..>2360)
                     /locus_tag="VJC18_11560"
     CDS             complement(1217..>2360)
                     /locus_tag="VJC18_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46061.1"
                     /translation="DTLDENTSYTITVAGIKDASSNCITDSTNTFTTAQELPVNSVTK
                     TSVSSEEDGGTTQIELLINMEIEALPAVEVAMSESGAAEPAATADADCVASGDDTLCT
                     VTVSGVAGCATPTDYEVTLSAEGYEDYVIHFNSADNEFEEATTVSDNDCWIQDHSAGD
                     ADNEFDWSASGGNLVMTMDNLGTGSHDIYYDQPFADSKEGAFVVYISDLETPATNGTN
                     FDLVMVQATNSQFDGLNGIVSGYNGLADPAYSGWGSVYEGGNGYQWGIPLETGDLNDI
                     ADVYDGYYICMTAYDDLLTTYISTDGDNYYKLTEDNMRCDTGTSCNIDQIDSRSIADW
                     DDLRLSIQVGSDNGAGDGTLTAKFGFARFRTSNLNGSMADCPRMNR"
BASE COUNT          586 a          654 c          519 g          601 t
ORIGIN      
        1 atttccctga cccccgcatc aaacaccgcc ttcatgaaag agttgccata atcaaaaaaa
       61 taggttcccc gagctaccag cgtgctgaca cattcaaagt gtttgcgcaa actttgatcc
      121 acaagttttt tgaaaccatc acgatcctcc gccaaatatt ttgtgcgttc ctcaaacgtg
      181 attccctgcg gacaataccc cccgtcgtac acagaatgac acgatgtctg atcggacaaa
      241 agttcaatgc tgatggtgtt tgtgacagcg tattccagca aatcaaccac attgccatga
      301 tacgcaatcg agagaggttg cttttgcgcc atcgcttttt gcgccatcgc aaacgcctct
      361 gcccgatcag ccgtcacctt cgctacccac ccctgatcat gacgcgtgtt gattcgcgat
      421 gcgtcaacct cggcaatcag agccaccccg cccgcaatct ctaccgcctt gggctgtgcc
      481 ccactcatcc cgcccaagcc tgaagaaaca aagagatgtc cccgcaggtc ctcatctggc
      541 cccaaaccga agcgttgacg gccagcattc aagaccgtgt tgaatgtgcc gtgcacaata
      601 ccctgtgggc caatgtacat ccaccccccc gccgtcatct gcccataatt ggccacaccc
      661 aactgcatgg cacggtgaca atcagtttga ttatcaaaca agcccaccat gaggccattg
      721 gtatttatta cacgcggcgc cgagggtttt gaggcaaaga gccccaacgg gtgaccggag
      781 gcgacaacca gcgtctgctc ttcggtcagg attttgagat aggcattgat gagttgatac
      841 tgcatccaat tttgcaccac ctgccctgtt tcaccatatg tcaccagctc gtaagggtac
      901 agcgccaccc taaaatcgag attgttatcg atcatcacct gcaaggcctt gcctgccaag
      961 catttgccct catactgatc tatcggtttg ccaatgatat tgccccgcgg gcgaaagcgg
     1021 tagccgtaaa tacggccgcg ggttttgagt tcttccaaaa attcctggcc caatgtttga
     1081 tggtactttt tgggaatgta ccgaagggcg tttttgaggg cgagctttgt ttcatttttg
     1141 ttgagtgtga agccacggtc aggcgccctg cgcacaccgg gctcaaaagg gggaattgac
     1201 tgcggcacat tttgaatcac ctattcatcc tcgggcaatc ggccatggag ccattgagat
     1261 ttgatgtgcg gaaacgcgca aaaccaaatt tggcagtcaa cgtcccatcc cctgccccat
     1321 tgtctgaacc aacctgaatg gagagtctta aatcatccca gtcggcgatg gaacggctgt
     1381 ctatctgatc gatgttgcaa ctggtgcctg tatcacacct catattatct tctgtcagct
     1441 tataataatt gtctccatca gtgcttatgt atgtcgtcaa cagatcatca taagcggtca
     1501 tgcagatgta gtagccatcg taaacatctg ctatatcatt caaatcacca gtttccagag
     1561 gaatgcccca ttgataacca ttaccgcctt cataaacaga accccaaccg gaataagcag
     1621 gatctgccaa tccgttgtaa ccgctaacga tgccgttcag gccgtcgaat tgcgaattgg
     1681 ttgcctgaac catcacaaga tcaaaattgg ttccgttggt cgccggagtt tcaagatcag
     1741 atatataaac aacaaacgcc ccttcttttg aatcggcaaa gggttgatcg taatatatgt
     1801 cgtgtgatcc cgtgccaaga ttatccatgg tcatcacgag attgccacca gaagcactcc
     1861 agtcaaattc gttgtctgcg tcgccagccg aatgatcctg tatccaacaa tcattatcac
     1921 tgactgtggt ggcctcttca aattcgttat cagctgaatt gaaatgtatg acataatctt
     1981 catatccttc agccgacaag gtaacttcat aatcggtggg tgttgcacaa ccggcgacgc
     2041 cagacacagt caccgtacag agcgtgtcat ctccgctggc aacgcagtcc gcatctgccg
     2101 tcgctgcagg ttcggcagct ccgctctccg acatggcaac ttctacagca ggcaacgctt
     2161 caatttccat attgatgagt aattcaatct gcgtcgtacc accgtcttct tctgaactga
     2221 ctgaggtttt tgttaccgag ttaacaggca attcctgcgc tgtcgtaaac gtgtttgtag
     2281 aatccgtaat gcaattgcta gacgcatctt tgatgcctgc cacagtgatg gtatagctgg
     2341 tgttttcgtc cagagtatca
//
LOCUS       DATDUN010000831         1984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_329085, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000831 DATDUN010000000
VERSION     DATDUN010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329085"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VJC18_11565"
     CDS             <1..216
                     /locus_tag="VJC18_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46062.1"
                     /translation="LVIPAKAGIHFTLLDSCISAIGLIPLAGRRTVFRHFLFTIHYSI
                     FIGSFPYHPKSQNAPSTDGLQAVAVSG"
     gene            249..1223
                     /gene="holA"
                     /locus_tag="VJC18_11570"
     CDS             249..1223
                     /gene="holA"
                     /locus_tag="VJC18_11570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HLD46063.1"
                     /translation="MDKPVIQLLINNEEFLCRRHLKHIEKKIIDEGTKDFNLSKVSAQ
                     DLSVGQLIDLFNTLPMMATYRTVIVRAFDEITKNNEAAKKEAATLADYFLNPNPSTHI
                     IMICSKIDKRTTLYKNIAKQGDVIEFKPLYADKVPAFVAAEAKAMGLVLERGCAEQIA
                     EVVGLNLMSIVTELEKLAVYVLPEKRVTLKQVGELICFATVDNVFLLSEHIACKAYAK
                     ACEVFDKMCEQGEPVVKTTSLVITHFRKLMLLKSAQQECLPESEWAGLLGVHSYFIKK
                     YEQQANAYQVPQLKRVYGLLMEASVQLRTLSVSDGTVFETFLQKVCVG"
     gene            complement(1232..1663)
                     /locus_tag="VJC18_11575"
     CDS             complement(1232..1663)
                     /locus_tag="VJC18_11575"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLD46064.1"
                     /translation="MSQKTFSKQQIKKYLRICNTDACHKHIGIKATKITLKEARLELK
                     LSPRHLNFENCVHGGILSFLMDTVMGFAVYPHLHEGERILVVDLKVTYLKAALLTMKK
                     ITGRSRLVARTKRFAVSEGEIISPSGDILCKGMGTYAVITR"
     gene            complement(1701..>1984)
                     /locus_tag="VJC18_11580"
     CDS             complement(1701..>1984)
                     /locus_tag="VJC18_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46065.1"
                     /translation="TDVCGQTYLTDFVGGTPEGQALGFSAHYFTNTASTHAGTRNGYQ
                     FLSLGRFISREPVDPSSEAFRQSYKAAYEQLPLPWYTRLGIIIGKWLED"
BASE COUNT          489 a          478 c          515 g          502 t
ORIGIN      
        1 cttgtcatcc cagcgaaagc tgggatccat tttactcttc tggattcctg catttcagcc
       61 ataggactga tcccgcttgc gggacgcagg acagtatttc gacatttctt gttcaccatt
      121 cattattcga tattcatagg gagcttcccg tatcacccca aaagtcaaaa cgcgccttcg
      181 actgacggat tacaggcggt cgccgtttct ggttgaatac cggatcagac tccattatcc
      241 acccccttat ggacaaacct gtcatccaac tcctcatcaa caacgaagaa ttcctgtgcc
      301 gccgccacct caaacacatc gaaaaaaaaa tcatcgatga gggcacaaaa gattttaatc
      361 tttcaaaggt ctctgctcag gacctgtcgg tcggtcaact gatcgacctg ttcaataccc
      421 tgcccatgat ggccacatac cgtaccgtga tcgtgagggc atttgatgaa atcaccaaga
      481 acaacgaagc ggcgaaaaag gaggcggcca cactcgctga ctattttttg aatcccaatc
      541 cctccactca catcatcatg atttgttcca agatcgacaa acggacgacc ttgtataaaa
      601 acattgccaa acagggagat gtgattgaat tcaaacccct gtacgccgat aaggtacccg
      661 ctttcgttgc ggccgaggcc aaggcgatgg gacttgtttt ggagcgtggt tgtgctgagc
      721 agatcgcgga ggttgtgggg ctcaatctga tgagcatcgt aacagaattg gaaaagctgg
      781 ctgtgtatgt tctgccggaa aaaagggtaa cgctcaagca ggtgggtgaa ttgatctgct
      841 ttgccacggt tgataatgtc tttttgctct ccgaacacat tgcctgtaag gcctacgcca
      901 aggcttgcga ggtgtttgac aagatgtgtg agcagggcga acctgtggtc aaaacgacga
      961 gtctggtcat tacccatttt cgcaaattga tgctcctgaa atcggcccag caggaatgtt
     1021 tgccggagag tgagtgggcg gggctgttgg gtgttcattc ctacttcata aaaaagtatg
     1081 aacagcaagc caacgcgtat caggtgccgc aactcaagcg tgtgtacggg ttactcatgg
     1141 aggcctcggt gcagttgcgc acgctgtctg tgtccgatgg tacagtgttc gaaacctttt
     1201 tgcagaaggt gtgtgtgggg tgaaggtggg gctaccgcgt aatcaccgcg tacgttccca
     1261 tgcctttgca caggatgtca ccggagggag aaatgatttc gccttcgctc accgcaaagc
     1321 gtttggtgcg agcgactaac cgtgaacgac ccgtgatttt cttcatggtg agcagggcgg
     1381 ccttcagata agtcactttc aaatccacca ccagaattcg ttcgccctca tgtaaatggg
     1441 gatagaccgc gaaccccatc acggtgtcca tgaggaacga caggatgccg ccgtgcacac
     1501 agttctcaaa attgagatga cgcggtgaga gtttgagttc aagcctggcc tctttgagcg
     1561 tgatctttgt ggctttaatg ccgatgtgtt tatgacacgc gtcggtgttg cagatgcgca
     1621 gatatttttt gatctgttgt ttggaaaaag ttttttgaga catgaggtcg tataagttgc
     1681 aaaactcaaa agatcaaaga tcaatcctca agccacttgc caataattat tcccagtctc
     1741 gtgtaccaag gcaacgggag ttgctcatag gcggctttgt agctttgtcg aaaggcctcc
     1801 gaactggggt caacgggttc cctcgaaatg aatctcccaa gtgaaaggaa ttgataaccg
     1861 ttgcgggtgc cggcgtgggt gctggctgtg ttggtaaaat agtgcgcgct gaatccgagg
     1921 gcttggcctt cgggggtgcc tcccacaaaa tcagtcaaat aggtttgtcc acaaacgtcc
     1981 gttt
//
LOCUS       DATDUN010000832         2388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_121130, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000832 DATDUN010000000
VERSION     DATDUN010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121130"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /gene="clpP"
                     /locus_tag="VJC18_11585"
     CDS             complement(<1..109)
                     /gene="clpP"
                     /locus_tag="VJC18_11585"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033550.1"
                     /note="hydrolyzes proteins to small peptides; with the
                     ATPase subunits ClpA or ClpX, ClpP degrades specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLD46066.1"
                     /translation="MSQIYNQTLVPMVVENTHRGERAYDIYSRLLKDRII"
     gene            complement(185..1384)
                     /gene="tig"
                     /locus_tag="VJC18_11590"
     CDS             complement(185..1384)
                     /gene="tig"
                     /locus_tag="VJC18_11590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HLD46067.1"
                     /translation="MITTKIKDINSTRKKINITIAREKIQDYRTKACQKVSQTAKVNG
                     FRPGKVPNHILERFYGPQIDFECLNTMVSDTYLKALTEHKLTPMTEPKFDTEPLEPNK
                     DYSFSIELEIRPQFELKNYKGIKLKKKEAQVNDDDVVAELKRLQESLAQLAPTEDGTV
                     LTKGLVATIDFTGKIDGKVFKGGQAKDYVFEYGLGQLLSDFEKNIDGMKKNETKTFVM
                     DFPKDYFEKDLAGKKADYEVTLKNLHIKNLPPLDDELAKDVGKENLEQVKTELKDSLI
                     KRKERDFRREYAEEIKKHLQKEYQFDVPESLVAEETERTKQDKKEIQERFRLDLVLDT
                     IAHAENIQATQQDIEFRMITLAHVYKQPVEEIRKLYKQNNMMSSLAIQIVFDKTLERL
                     IDLAQFS"
     gene            complement(1534..1615)
                     /locus_tag="VJC18_11595"
     tRNA            complement(1534..1615)
                     /locus_tag="VJC18_11595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Leu,seq:tag)
     gene            1901..>2388
                     /locus_tag="VJC18_11600"
     CDS             1901..>2388
                     /locus_tag="VJC18_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD46068.1"
                     /translation="MNRRIIITMLIGLILLGQSTSVWAVQGKLKEALASYEAQEYSSA
                     LAHFVTLAEEGSSEAQYHVGRMYLLGQGVGKDLKISASWYQKAANQGNAKAQNDLGVM
                     YLRGIGVPQDYKKGFEWIGQAANAGLIDAQFSMGLLYAEGKGVRKNLQQAASWFEQAA
                     KQG"
BASE COUNT          530 a          514 c          646 g          698 t
ORIGIN      
        1 agatgatgcg gtctttgagc aggcgagaat agatgtcgta ggccctctca ccacgatgcg
       61 tgttctcaac caccatggga accagggttt gattgtaaat ttgtgacatg tgagtctgtg
      121 cgtgcgccca gcctgtgcgt atggggtaac aggaaatccc tacgcaagag cgtgctcaaa
      181 cacattatga aaattgagcc agatcgatga ggcgttccag tgttttatca aaaacaattt
      241 gaatagcaag tgatgacatc atgttgtttt gtttgtacag cttgcggatt tcttcgacag
      301 gctgtttgta gacgtgggcc agggtgatca tgcggaactc gatgtcctgc tgtgtggcct
      361 ggatgttctc tgcgtgggca atcgtatcga gaaccagatc gagtcgaaaa cgctcctgaa
      421 tttctttttt gtcttgtttc gtacgctcgg tctcttccgc aacgagtgac tcgggaacat
      481 caaactgata ctccttttgc aggtgctttt tgatttcttc ggcgtattcg cggcgaaaat
      541 cgcgttcctt gcgcttgatg agcgagtcct tgagctctgt tttgacttgc tccagatttt
      601 ccttgcccac atccttggcg agttcatcgt caagcggtgg cagatttttt atgtgcaggt
      661 tcttgagcgt cacctcgtag tctgcttttt tgccagccag atctttctcg aaataatctt
      721 tgggaaaatc catgacgaag gtcttggttt cattcttctt catgccgtca atgttttttt
      781 cgaaatccga caacagttgt cccaaaccgt attcaaacac gtaatccttg gcttgcccgc
      841 ccttaaagac tttgccgtcg atcttgccgg tgaagtctat ggtggcaacc aggcccttgg
      901 tgagaaccgt accgtcctct gtgggagcaa gttgtgccag actttcctgc aggcgtttga
      961 gttccgcgac gacgtcatca tcgttgactt gggcctcttt ctttttgagc ttgatgccct
     1021 tgtaattttt gagttcgaat tgaggcctga tttcaagctc gatggagaag gaatagtcct
     1081 tgttgggttc aaggggctcg gtgtcgaatt ttggctcggt catgggcgtg agtttgtgtt
     1141 cggtaagggc cttaaggtag gtgtctgaga ccatggtgtt gagacactca aaatcgattt
     1201 gagggccata gaatctttcg aggatatgat tgggcacctt gcccggtctg aaaccgttga
     1261 ccttggcagt ttgactgact ttttgacagg ccttggtgcg gtagtcctga attttttccc
     1321 tggcgatcgt gatgttgatt ttttttctgg tgctgttaat gtctttgatt tttgtggtga
     1381 tcatttttgt gcttccttaa acatagggtt gtatcatgca aaattttgcc aaagggtcat
     1441 tcccaacgat ctattcccga ttgccattct cattatgttc attttgaaaa tggaaaatgg
     1501 aaaatagagg gaggctgtca aaatcagcct ctgtgcgagc ggggggactc gaacccccat
     1561 gccttgcggc gccaggttct aagcctggtg cgtctgccag ttccgccaca ctcgcccgcc
     1621 ccttagaaaa acacccatct gcggcgttgt cctcaaacct gcaatcctca cgtatttcca
     1681 tatacgctcc ggttgcaggt ttgaggacgc cttgcatctg ggcgtttttt taaggggcgg
     1741 gaaaagcaat gcttttcaga gaaacgcccc gaagagggta tgtcccatca gaactttttg
     1801 ccccccagaa cacagtggac aggccacgta acaagttgta tgaaataaat caatggtttg
     1861 aagctagttt ttccaatcaa aaaacaagga tggaagtaaa atgaatcgac gcatcatcat
     1921 cacaatgctt attggcttga tattattagg gcaatcgacc tcagtgtggg cagtgcaggg
     1981 taagctgaag gaggcactgg caagttatga ggcccaggag tactcgtctg ccttggctca
     2041 tttcgtgacc ttggcggagg aggggagttc tgaggctcaa tatcacgttg gtcggatgta
     2101 tttattgggg cagggcgttg gcaaagattt gaaaatatct gcgtcttggt accagaaggc
     2161 ggcgaatcag ggcaatgcca aggctcagaa cgatttgggt gtgatgtatt tgagggggat
     2221 tggtgtgccg caggattaca aaaaagggtt cgagtggatc ggtcaggctg cgaacgctgg
     2281 tttgatcgac gctcagtttt ccatgggtct tttgtacgcg gagggtaagg gggttcgaaa
     2341 aaatctgcag caagccgcga gttggtttga gcaggcagca aaacaggg
//
LOCUS       DATDUN010000833         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_453329, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000833 DATDUN010000000
VERSION     DATDUN010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453329"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VJC18_11605"
     CDS             complement(<1..1188)
                     /locus_tag="VJC18_11605"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HLD46069.1"
                     /translation="MNQLTPFTRHLLGDDRLKQLVSPFVVSGEDVTTQALAYLQNSHR
                     VVTELMQKDVSPLDLHRLRSLMMDELIRGLWQYFSSQPDSRSVVEQTSFALMATGGYG
                     REEMSLLSDIDLLFVVPSHRSDHYQQLAQKLLYVLWDLKLDVGHAVRTFDETLQYLHD
                     DHTIFTALLNLRLIVGDASLFENLLCQRDEALQDLAVKRRIFLAKYDERQVRLKKFGD
                     TVYLLEPHIKEGEGGLRDLQLIRWMAQIVGVGSDYSCLCEAGFLDGVARDQLQLSLCF
                     LLTIRNKLHAVLGRKSDQITFDAQIILAKELDFEDDAVVLGVEKFMQAYYTVASQVNR
                     HVKTLIQKLASPLGLGLAPTQSQIIDQVLDDHFCIKEAQIAVRDDDVFEKDITHLMDA
                     FLHV"
     gene            complement(1219..1392)
                     /locus_tag="VJC18_11610"
     CDS             complement(1219..1392)
                     /locus_tag="VJC18_11610"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLD46070.1"
                     /translation="MSKEFFPPRPQSKPIIYAYEVTNPQYAGLLKVGYTTVDVQTRVA
                     QPYSILRNLLLNK"
     gene            complement(1402..2439)
                     /locus_tag="VJC18_11615"
     CDS             complement(1402..2439)
                     /locus_tag="VJC18_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HLD46071.1"
                     /translation="MELKDYSSAIRKIKDAILQSRYTAAALANRELLSLYFSVGRYVS
                     ENTRNRLWGTGAIEVISERLQQELPGLRGFSTTNIKNMRLFYESWEDFLPNRQLTTDD
                     LRGELFTRVGFTHHMEIILKVKGQNARLFYVEQCATGFWSVEKLKYHIKENLFSQKGA
                     LQNNFTKTIPDQTFRAKTLHAFKGELLLDFVNIEDPDEEPDERVLEHAIVNNIKKFIM
                     ALGSDFSFVGNQYRLLVDGNEFFIDLLFFNRRLQSLVALELKKGKFKAEYAGKMNFYL
                     SALDELIKMPHENPSIGIILCKEKNHKIVEFSFRDTSKPMGVALYKTSAKLPAQYRNI
                     LPDAEKLKRLL"
BASE COUNT          655 a          592 c          559 g          675 t
ORIGIN      
        1 cacgtgcaga aacgcatcca tcaagtgtgt gatgtctttc tcgaacacat cgtcatcacg
       61 cacggcaatc tgtgcctctt tgatacagaa atgatcatcc aacacctgat cgatgatctg
      121 tgactgcgtc ggcgcgaggc ccagccccaa aggggaagcc aatttttgga tgagcgtttt
      181 aacgtgccga ttcacctgtg aggccacggt gtaataggcc tgcatgaatt tttcgacccc
      241 cagaacaacc gcgtcgtctt caaaatccag ttctttggcg aggataatct gggcatcgaa
      301 ggtgatttga tcgcttttgc gaccaagcac ggcatggagc ttgtttctga tcgtgagcag
      361 aaagcaaagt gacaactgca attggtcccg ggcaacacca tcgagaaagc ctgcctcaca
      421 caaacatgaa taatcgctgc ccacaccaac gatctgagcc atccaacgaa tgagctgcaa
      481 atccctgagt ccgccctcac cttccttgat gtggggctcc agcagataaa ccgtatcacc
      541 gaattttttt agtcgcacct gccgctcatc atatttggcc agaaaaatcc gccgtttgac
      601 agccaggtcc tgcagggctt cgtcacgctg gcaaagcagg ttttcaaaca ggcttgcatc
      661 acccacaatc aaacgcaaat tcaacagcgc cgtaaagatc gtatggtcgt catgcaggta
      721 ttgaagggtc tcgtcaaacg tgcgcacggc gtgccccaca tcgagcttga ggtcccacaa
      781 gacgtacaag agtttttggg ccaactgctg ataatgatcc gatcgatgtg acggcaccac
      841 aaacagcaaa tcgatgtcgg acaagagcga catctcctcg cggccatacc cacctgtggc
      901 catgagggcg aaagaggttt gttccacaac ggatcgcgaa tctggctgag atgaaaaata
      961 ctgccataac ccgcgaatga gttcgtccat catcagggag cgcaggcggt gcaaatccag
     1021 cggagagaca tctttctgca tcagctcagt caccacgcgg tggctgtttt gcagatacgc
     1081 caaagcctgc gtggtcacgt cttcgccact cacgacaaag ggggacacca gttgcttcaa
     1141 acgatcatct cccaaaaggt gtctggtgaa gggggtcaac tggttcatag ttctgctctc
     1201 aggtatttga gagtacagtt atttatttag caataaattc ctcagtattg aatatggttg
     1261 tgccacgcgg gtctgcacat caaccgtggt gtatcccact ttgagcaaac cggcatattg
     1321 cgggttggtg acctcatagg cgtagataat tggctttgat tgtgggcgtg gtgggaaaaa
     1381 ttctttgctc ataatgatat cttataaaag tcttttcagt ttttcagcgt ccggcaaaat
     1441 atttctgtat tgtgcgggaa gttttgctga cgtcttgtaa agagccactc ccataggttt
     1501 gctagtatct cgaaatgaaa actcaacgat tttatgattt ttttctttgc acaaaatgat
     1561 accaattgaa gggttttcgt ggggcatttt tatcaattca tctaacgctg acagataaaa
     1621 gttcattttc cctgcatact cggctttgaa ttttcctttt ttaagttcca aggcaaccag
     1681 gctttggagt ctccgattaa aaaaaagcag atctataaaa aactcattgc catctacaag
     1741 tagccggtat tggttaccaa caaaactaaa atccgaaccg agtgccataa tgaacttttt
     1801 gatattgttg acaatggcat gttccaaaac ccgttcatca ggttcttcgt cagggtcttc
     1861 tatgttgaca aaatcgagca acagctcgcc tttgaaagcg tgaagcgttt tcgctcgaaa
     1921 cgtctggtcg ggtatcgttt ttgtaaaatt gttctgcaac gcaccttttt gcgagaacaa
     1981 attttctttg atgtggtatt tcaatttttc aacactccag aaacctgtag cgcactgttc
     2041 aacataaaac agtcgggcgt tttgcccctt tactttcagg ataatttcca tgtgatgagt
     2101 aaaacctacg cgagtaaaaa gctctccccg caaatcgtca gttgtcaact gacgatttgg
     2161 aagaaaatct tcccaagatt catagaagag tcgcatgttt ttaatgtttg ttgtggaaaa
     2221 tcctctaaga cctggtagtt cttgctgtag cctttcagaa atgacttcta tggcaccagt
     2281 gccccacaag cggttacggg tattttcaga aacataccgg cccacagaaa aataaagaga
     2341 aagcaactcc cgattggcaa gtgccgctgc cgtgtagcgg ctttgtaaaa tggcgtcttt
     2401 tatttttctg atggcgctcg aatagtcttt taattcgatg gttcggatcg tttttttact
     2461 catcgcttgc ctccatcaga c
//
LOCUS       DATDUN010000834         3176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_329305, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000834 DATDUN010000000
VERSION     DATDUN010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329305"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VJC18_11620"
     CDS             <1..1330
                     /locus_tag="VJC18_11620"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HLD46072.1"
                     /translation="EIFSPFSADNIKRAKPDLVVVGNAITRTNPEADYLLASQIPYVS
                     MPQALQLFFFEDKEVIVVTGTHGKTTTTAIMSHLLTELGQDPSFLVGGVAKNQKRNFR
                     LGSGKYFVIEGDEYDTAFFEKTPKFLHYQPCHVIATSLEFDHADIYENVGQIEDAFSK
                     LFDLIPRDGSLHFCSAFPRLEPLIRSKMGKISYFIRRYGTDAVSWQIANYQALGGGSA
                     FDLCVNGRTLLTITSPMTGLYNAQNVVACFSVLEHLGFGLPQAVEALQRFEGVKRRQE
                     ELYADEEWIVVDDFAHHPTAVSETIQAIKEKYPHRELVALFEPRSNTTRRNIFLHEYI
                     QAFLLADQVYLAPVHQPEKVPEGQALDVVAIISQLCEHGIRAEGPLSCDAIINKVLEQ
                     HTQPSVVLIMTNGSFDGLGEKLVARLGHENTKTRGMKGKFNRAMSNRQLL"
     gene            1446..2789
                     /locus_tag="VJC18_11625"
     CDS             1446..2789
                     /locus_tag="VJC18_11625"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HLD46073.1"
                     /translation="MSTNQKVSVHQDTLVIGGGIAGIQASLEIADAGKKVFLVEKSGT
                     IGGHMAMFDKTFPTLDCAACILTPKMVDVGQHDNIELLTNSEVKSVIGVPGNYKVKIL
                     KRARRVNEKTCTGCGTCAEKCPGRALSEFDSGTTTRKAIYIPFAQAVPNKYLIDPAVC
                     HYVLKGKCGVCVKNCPVPDCINLDEKDQEIELTVGNIIVATGFKPFDAKRAEQFGYGL
                     FPNVVTSLELERLINAGGPTGGEIKFRKKDKFGNWVFTPEGDSPKSVAIIHCVGSRDE
                     NYNKYCSTVCCMYSLKLAHLVKEKLHEAPVYEYYIDMRSFGKGYEEFYNRIKNEGVSV
                     LRGSTVKIDENNGKLVVKSQDVMSGNLITQEVDMVVLAVGLEPQAGAKDLAKMLNIPV
                     DEHGWFTEAHSLLDPVNTVNSGINIAGVCQGPKDIPDSVVQASAAAARVLQGMMG"
     gene            2881..>3176
                     /locus_tag="VJC18_11630"
     CDS             2881..>3176
                     /locus_tag="VJC18_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLD46074.1"
                     /translation="MSTKINSNVVDTVDKLGEQNMEMCFNCSKCTAVCPMDECLLPRT
                     LFRYALLGMTDKIKKEQEKIYSCLLCRLCEQSCPRGVKIADNIRMMRRYLNNEV"
BASE COUNT          818 a          722 c          839 g          797 t
ORIGIN      
        1 cgaaatcttt tctcccttct cagccgacaa catcaaacgg gcaaagcccg atctggttgt
       61 ggtaggcaat gccattacca gaaccaaccc cgaagcagat tatctgcttg cctcgcaaat
      121 tccctatgtg agcatgccgc aggctttgca gctgtttttt ttcgaagaca aagaggtcat
      181 cgtcgtcacg ggtacgcacg gcaaaacgac caccacagcg atcatgtcgc atttgctgac
      241 tgagcttgga caagacccgt cttttttggt gggaggggtt gccaaaaacc agaaacgaaa
      301 ttttcgtttg ggttccggga aatattttgt gattgaggga gatgagtacg acacggcttt
      361 ttttgagaag acaccaaagt ttttgcatta ccagccgtgc cacgtgatcg ccacatcgct
      421 tgagtttgat cacgccgata tttacgagaa tgtgggacag atcgaggacg ccttttccaa
      481 actctttgat ctcattcccc gagatggttc cctgcatttt tgctcggcat ttcccaggct
      541 ggagcctctt attcggtcaa aaatgggcaa gatttcgtat tttatcaggc gctacggaac
      601 agatgctgtt tcgtggcaaa tcgcgaatta tcaagcattg ggtggtggca gtgcatttga
      661 tttgtgtgtc aacggcagga cgttgttaac cataacatca ccgatgacgg ggttgtacaa
      721 cgctcagaac gtggtcgctt gtttctcggt tttggagcat ttgggatttg gcttgcctca
      781 ggcggtagag gcgttgcagc gttttgaggg ggtcaaacgg aggcaagagg agttgtatgc
      841 cgatgaagaa tggatcgtgg ttgacgactt cgcccatcac cccacggccg tgagtgaaac
      901 tattcaggcc atcaaggaaa agtaccctca tcgcgagttg gtggcccttt ttgagccacg
      961 ttccaacacg acacggagaa atattttttt acacgaatac attcaggcct ttttgctggc
     1021 cgatcaggtg tatctggcgc cagtccatca accggagaaa gttccggagg gacaggctct
     1081 cgatgtggtg gccatcattt cgcagctctg cgaacatggc atcagggccg aggggccttt
     1141 gagctgtgat gccatcatca acaaagtgtt ggaacagcac acacaaccct cagtggtgct
     1201 catcatgacc aatgggtcgt ttgatgggtt gggggaaaaa ttggtggcaa ggctcggaca
     1261 cgaaaacacg aaaacacgag ggatgaaggg aaaattcaat cgagcaatga gcaaccggca
     1321 attgttgtaa ctgacaatta tcagcttgca gaatgacttt aatcaaacta aatcgggttg
     1381 cgattcacgc aaacttgagt atcatgcctg cgatttaatt gtatattttt ctttctggag
     1441 tttttatgtc aactaatcaa aaagtctctg ttcatcaaga tacgctggtc attggagggg
     1501 ggattgctgg tattcaggcc tctctggaaa ttgctgatgc gggcaagaag gtttttttgg
     1561 tcgaaaaatc aggcaccatc ggcgggcaca tggccatgtt cgacaaaacc tttcccacac
     1621 tcgattgtgc ggcctgtatc ctcacgccga agatggttga tgtgggccag cacgacaata
     1681 tcgaactcct aaccaactcc gaagtcaaat ctgtcattgg ggttcccggt aactataaag
     1741 ttaaaattct taaacgtgcc cgccgtgtca atgaaaaaac ctgcaccggt tgtggcacct
     1801 gtgccgaaaa atgtccggga cgggcactga gcgaatttga ttctggcacg accacccgca
     1861 aagccatata tatcccgttt gcgcaggcag tgccaaataa gtatctgatt gaccccgctg
     1921 tgtgtcatta cgtgctcaag ggcaaatgcg gcgtgtgtgt caaaaattgt cctgttcccg
     1981 attgcatcaa tctggacgaa aaagatcagg agatcgaact gactgtcggc aacatcattg
     2041 tcgccacagg cttcaaaccg tttgacgcca aacgcgcaga acaatttggt tacggactct
     2101 tccccaatgt cgtgacatca ctggagttgg aacgactcat caatgcgggt ggtcccacgg
     2161 gtggcgaaat caaattccgt aaaaaagaca agtttggcaa ttgggtgttc acaccagaag
     2221 gtgattcgcc caagagcgtg gcgatcattc attgcgtggg gagccgcgac gaaaattaca
     2281 acaaatattg ttccactgtt tgttgtatgt actccctcaa gctcgcgcat ctggtcaaag
     2341 aaaaactgca tgaggcgcca gtgtacgagt attacatcga catgcgatcg ttcggcaaag
     2401 gctatgagga attttacaac cgcatcaaaa atgaaggtgt gagtgtcttg cgtggcagta
     2461 cggtcaagat cgatgaaaac aacggcaagt tggtggtgaa gagtcaggat gtcatgtcgg
     2521 gcaacctcat cacgcaagaa gtcgatatgg tcgtgttggc cgtgggtctt gagccgcagg
     2581 cgggcgccaa ggatttggct aaaatgctga acatcccggt tgatgaacac ggttggttca
     2641 ctgaggctca ttcgctcttg gatcctgtca atacagtcaa cagcggcatt aacattgcgg
     2701 gtgtgtgtca ggggccaaag gacattcccg attcggtcgt gcaggcgtca gccgccgcgg
     2761 caagggtatt gcaggggatg atgggataaa tcgtggatcg atgatcgctg atcgtggatc
     2821 gtacaaggtt attggatgat cacgattcac gattcacgat tcacgattca cgagagtatt
     2881 atgtcaacaa aaatcaattc aaacgtcgtc gacactgtcg acaaactggg cgagcagaac
     2941 atggagatgt gctttaattg tagcaagtgc acagctgtgt gtcccatgga tgagtgtttg
     3001 ctcccacgca cattgtttcg ctatgccttg ctgggcatga ccgacaaaat caaaaaagag
     3061 caggagaaaa tctattcctg tctgctttgc cgtttgtgcg aacaaagctg tccccgtggt
     3121 gtcaagatcg cggataacat ccgcatgatg aggcgctatc tcaataacga ggtgtt
//
LOCUS       DATDUN010000835         1116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_329329, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000835 DATDUN010000000
VERSION     DATDUN010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329329"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VJC18_11635"
     CDS             complement(<1..1116)
                     /locus_tag="VJC18_11635"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HLD46075.1"
                     /translation="MLFAAIPKKQNKWRFIALLSFFTKLFNLLLLRRLSTAIDPLLSP
                     SQAGFRKGRNTLQHITTLQCLLGLAHSSPDFPLALGFVDFANAFPSVSWDAIQGALDA
                     FHVPPFLASKIMSIIRNSTGQVGRANTQFNIGKGVMQGDTLAPFLFILVLDRVLNDSV
                     GHLSHLGVPISRGTTLTHLAFADDVVFASHSTTNLSTMLSSLATRASSIGLLLNFSPN
                     KTEALLTNCEGPVVVSTPTSTTELRKTTKYTYLGLPLCPKAAVDQRCAMALTANTRLL
                     PLYASPSLSHRTKLLIFQTLAEPVLLYGLACLPLTPPLLKRLQGTYTTLLRMVVTGCS
                     GSAAHITRTIIYDNNKIPEITTKIWELRYRFLQRWLLP"
BASE COUNT          279 a          205 c          381 g          251 t
ORIGIN      
        1 ggggaggagc cagcgttgga ggaagcggta gcgcaactcc cagattttcg tcgttatctc
       61 tgggattttg ttgttgtcgt agatgattgt tcgtgtgatg tgagctgcgc taccagagca
      121 accagtcaca accatgcgga ggagagtggt gtaggtgccc tgaaggcgct tgagtagagg
      181 gggtgtgagg gggaggcagg caagcccgta gaggagtaca ggctctgcca gtgtttgaaa
      241 aatcaaaagc ttagttctgt gcgatagcga gggggacgcg taaagaggga ggaggcgtgt
      301 gttggccgtc agggccattg cgcatcgttg gtccactgcc gctttgggac agaggggaag
      361 accaagatac gtgtacttcg ttgtttttct tagttctgtt gttgatgtgg gggtggaaac
      421 aacaacgggg ccctcgcaat ttgtgagaag tgcctccgtt ttgtttgggg aaaaattgag
      481 aaggaggcca atcgaggagg cacgagtggc gagggaggac aacatcgtgg aaaggttggt
      541 ggtggagtgc gaggcgaaaa cgacatcatc tgcaaaggcc aaatgtgtga gagttgttcc
      601 acggctaatc ggcactccaa gatgggagag gtggccgacc gagtcgttga ggacacgatc
      661 cagcactaaa atgaaaagaa atggggcgag ggtgtcacct tgcatcactc cttttccgat
      721 gttgaattgt gtgttcgctc ggccaacttg gcctgtactg tttctaatta tgctcataat
      781 tttggatgca aggaagggag gaacatgaaa agcgtcaagt gcgccttgga ttgcatccca
      841 agacacactg gggaacgcat tcgcaaaatc gacgaaaccc agggcaagtg gaaagtccgg
      901 ggagctgtgg gccagcccca ggagacactg caacgtggtg atgtgttgca aagtgtttcg
      961 tcctttacgg aatccagctt gggaggggga gagcagaggg tcgatggcag ttgagaggcg
     1021 gcggaggaga agaaggttga aaagttttgt gaaaaaagag agaagggcaa tgaagcgcca
     1081 tttgttttgt ttttttggga tcgcagcaaa cagaat
//
LOCUS       DATDUN010000836         3682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_329549, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000836 DATDUN010000000
VERSION     DATDUN010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329549"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1857)
                     /locus_tag="VJC18_11640"
     CDS             complement(142..1857)
                     /locus_tag="VJC18_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HLD46076.1"
                     /translation="MKFKKHFFRTCPLFVLLLLSCSAQSAPANATDHKSVTLTIAHVN
                     DTHSHIEPTTLNLKFDSLSAYVQAGGLPRLNAKIKELRQNDPNLLFLHAGDLFQGTIY
                     FQKFQGLADLYFFEAAGLDALTVGNHEFDKGPDVLNSFAEQADFPLLAANIVIKEGSS
                     LTDKIRPFIIKEVGGEKIAIIGLGTPQTKFISSPGELVSFDNPVTVIKKQIEQHSRIG
                     INKILVLSHLGFEADIRLARSVPEIDVIIGGHSHTQQGPFKKLGLHSFGPYPLIVQNP
                     ANQNTLIVQAWQNACELGVLHVTFDEQGVISQYSGNPELLVDDDFLTKNAAGEKVPVT
                     AVIKKQILTLIDKDPLIEVVEEDPAVMPKYHEFTASLAQLKQTVVGHAQDDLIRGPNR
                     GPGILMTDAMLWKTKNLGVNVALLNSGGARDNIFAGPITVDRIYAAHPFGNTLVIVEL
                     SGQELLAVLDENSQLYAGKGSPPYVSGMRFSIDKKNPKGKRITELQIRAADHQWVNVD
                     NHKLYKIVIPSFLANGGDYNDTLKNAKNKLDTGFIDAEILIDYVKGMKVIKESDEVRI
                     KIIKK"
     gene            2061..2507
                     /locus_tag="VJC18_11645"
     CDS             2061..2507
                     /locus_tag="VJC18_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLD46077.1"
                     /translation="MQTTVNQLLKRKGNQIWSVAPTDSVDKAVKLMAQRKVGALMVMK
                     DNAVVGVFSERDLARNMAHKGFSNPNIPVSEMMSRQVYYVNPTMTMVDCMKFMTEKRI
                     RHLPVMENNKLVGVVSIGDVVNSIIQDQESEIKDLENYIIGGTFQG"
     gene            2614..>3682
                     /locus_tag="VJC18_11650"
     CDS             2614..>3682
                     /locus_tag="VJC18_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46078.1"
                     /translation="MAVLCGSLSMLSGFGGSSAAWADTLSLVRGLAKEGVQLLPQGRV
                     LPESSAELAVNPTRRATEYLYSPHQVPAHLHRSGKIREDNSSSSTLTSDGSIGTGRFG
                     SLPRANQEAVQCTSQAERLYNTGLTKVIDVLRNGDVHSLVTEGAEADLLTSLAACPAS
                     NRTLRGNIRYQLVTIYFMLERSESAKEQLQEMLSEFDEQRDEPGFLVRIINGLSCCYQ
                     LGLDDAVFKIMSAQLARFDRLYEGASLDRTQLSLIRRFLSVPSDINIEQKDLRLAVAH
                     FYRAQSALVKGNFEAARLELDCELASLGRAQKILFTNETVDPLGFRILLGAYVGVAAI
                     STVIFSHDSGADSLSADLRVAV"
BASE COUNT          887 a          809 c          961 g         1025 t
ORIGIN      
        1 gagcagtgtt gtcaaaatat tctccaaatg ggggccccgt attgatctca tctatatcga
       61 aaaaggcagg ggccgctcgc tgttatcgac cgttatagtc aaaagttgcg ctgtggtaat
      121 aaaaaaatgt cgattaacca attatttctt gatgattttg attctgacct catcgctctc
      181 ttttatgacc ttcatccctt tgacgtaatc gatcaagatc tcggcatcaa taaagccggt
      241 atcgagcttg tttttcgcgt tctttaacgt gtcattatag tcgccaccgt tggctaaaaa
      301 tgaagggatg acgattttat acagcttgtg attgtcgaca ttgacccatt ggtggtcagc
      361 agcccttatt tgaagctctg tgattctttt gcctttgggg tttttcttgt caatgctgaa
      421 cctcatgccg gagacgtatg gtggactgcc tttgccagcg tataactgtg agttctcatc
      481 caagaccgca aggagttctt gcccactcag ttcgacgatg accaaagtat tgccaaaggg
      541 atgggccgcg taaatgcggt caacggtgat gggaccggcg aagatattat cgcgcgcccc
      601 gcccgagttg agcagggcca cgttgactcc cagatttttt gttttccaca acatggcatc
      661 tgtcatcaga atgccgggcc ccctgtttgg tccgcgaatc aagtcatctt gcgcatgtcc
      721 cacaactgtc tgtttgagtt gggccaagga ggcagtgaac tcgtgatact ttggcatgac
      781 agcagggtct tcctcaacca cttcaatgag ggggtcctta tcaatcaggg ttaatatttg
      841 ttttttgatg acggctgtca cgggcacttt ttcgccagct gcgttttttg taagaaagtc
      901 atcatccacc agcaattcag ggttgcccga atattggctg atcacgcctt gctcatcaaa
      961 agtgacgtgc agcacaccaa gttcacacgc gttctgccag gcctgtacga tcaatgtgtt
     1021 ctgattggcg ggattttgca caataagggg atacggaccg aacgaatgta gtcccagttt
     1081 tttaaagggc ccctgttgtg tgtgtgagtg gccgccgatg atcacatcaa tttccggaac
     1141 cgaacgggcg agtctgatat ctgcctcaaa gcccaaatgt gacaacacaa gaatcttgtt
     1201 gatgcctatg cgactgtgtt gctcgatctg ttttttgatg actgtcacag gattgtcaaa
     1261 gctcaccagt tcaccaggtg aagaaatgaa ttttgtttgc ggtgtgccca gaccaatgat
     1321 ggcgattttt tctccgccca cctccttgat gatgaaaggc ctgattttgt ctgttagaga
     1381 agacccctct ttgatgacaa tgttagctgc cagcagaggg aaatctgcct gctcggcaaa
     1441 actgttcagg acgtctggtc ccttatcgaa ttcgtggttc cctactgtca gggcatcgag
     1501 tccggccgct tcaaaaaaat aaagatcggc cagtccttga aatttctgaa aataaatcgt
     1561 gccctgaaac aggtcacctg catggagaaa gagtaggttg ggatcgtttt ggcgcagttc
     1621 ctttatcttg gcattcaggc ggggcaggcc tccggcttga acatacgctg agagggagtc
     1681 aaatttaaga ttcagtgtgg taggttcaat gtgggagtgg gtgtcgttca catgggcgat
     1741 cgtgagggtg acggatttat ggtcagtcgc attagcgggc gcgctttggg cggaacagga
     1801 gagcaggagc aagacgaaaa gggggcaggt tcgaaaaaaa tgtttcttaa atttcattag
     1861 gccatgtatt tcacacccga ttttgttatg caatcatgta tgtgcggatg taattgtatg
     1921 gcccgggctc acttggttga cagaaatttt gcagaattac ccattgatct tctgataatc
     1981 attcattagc tttcattaac atattcaaga ccgctcacgt cagcgacttg ctgatgtcgg
     2041 cgtgtcaagg gaggcaattt atgcaaacga ctgttaatca attactaaag cgtaagggca
     2101 atcaaatctg gtccgtggcg cccacagatt ctgtcgacaa ggccgtgaaa ctgatggccc
     2161 agagaaaggt cggcgccttg atggtgatga aggataacgc cgtcgttggt gtgttttctg
     2221 agcgtgatct tgcccgcaat atggcgcata aagggttttc taatcccaat attcccgtga
     2281 gcgagatgat gagccgtcag gtgtactacg tcaatcccac aatgacgatg gtggactgca
     2341 tgaaatttat gaccgaaaag cgcattcgcc acttgccagt catggaaaac aacaagctcg
     2401 taggggtagt ttccattggc gatgtggtga acagcatcat ccaggatcag gagtcagaga
     2461 tcaaggatct ggaaaactac atcatcggcg ggacgtttca gggctgatgt gcccgtgatg
     2521 attaataaaa aactctcatt ttcagagcat taattccaaa aaaacacgca acttgtcgta
     2581 tgaatctacc gatgacggag accggaggtt tttatggctg ttctatgcgg ttcgttgtcg
     2641 atgctgtctg gttttggagg ctcatctgcg gcatgggcgg atacattgag tctcgttcgt
     2701 ggtttggcaa aggagggggt ccagcttctt ccccaaggtc gtgttttgcc agaaagctca
     2761 gctgaacttg ctgtgaatcc aactaggcga gcaactgagt atctttattc tccacatcag
     2821 gttccagccc atttgcatcg ttctggaaaa attcgcgagg acaattcatc gtcgtcaacc
     2881 ctgacatccg atggttcaat agggacaggg aggttcgggt cattgcccag ggcaaaccag
     2941 gaagctgttc agtgtacaag tcaggcggaa cgtctttata acacgggcct gaccaaggtc
     3001 atcgacgtcc tgagaaatgg tgatgtgcat tcgttggtta ccgagggggc agaagcggat
     3061 ctattgactt ccttggcagc ttgtcctgcg tcgaatagga ctcttcgggg caatatacgt
     3121 tatcaactcg tgacaatcta tttcatgctt gagcgtagtg aatctgcgaa ggaacaattg
     3181 caggagatgc tatcggagtt tgatgaacaa cgagacgaac ccggatttct ggtcagaatc
     3241 attaatgggt tatcctgttg ttatcaactt ggtttagacg acgctgtttt taaaatcatg
     3301 agtgctcaac tggccagatt tgatcgtttg tacgaaggtg cgagtttgga tcgaacacag
     3361 ctgtccctaa tcagaagatt tctttctgtg ccttccgata tcaatatcga gcaaaaagat
     3421 ttaaggctcg ctgttgcgca tttctatcgg gcccagtccg ctctggtaaa aggtaacttt
     3481 gaggcggcta gactcgaact cgattgtgaa ctggccagtc tgggtagggc ccagaaaatt
     3541 ctctttacaa acgagacagt tgatccgctg ggttttcgaa tcttgctggg cgcgtacgtg
     3601 ggtgtggccg cgatcagcac ggtcatcttt tctcatgatt ccggggcgga ttcgttatct
     3661 gctgatttgc gggttgctgt gg
//
LOCUS       DATDUN010000837         4052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_164425, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000837 DATDUN010000000
VERSION     DATDUN010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164425"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..415)
                     /locus_tag="VJC18_11655"
     CDS             complement(215..415)
                     /locus_tag="VJC18_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46079.1"
                     /translation="MKLLSIRIDDFLLNTIQQEARNQKSTLVEVIRAALMNYFINKKD
                     IQDVQLAESRLNEDDLPFSENF"
     gene            1116..1784
                     /locus_tag="VJC18_11660"
     CDS             1116..1784
                     /locus_tag="VJC18_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLD46080.1"
                     /translation="MHTEKEEKVLDNLSTAEMVARKMTGKYGGAYFDDVKSYAHFGLI
                     LAARSFDDNRGCSFQTYATHCMKGAIIDGLRGEKVIRRIRSKVLLQKIEDRQKNVLDQ
                     LSVKPGTSIMNDIYVEHSTMLDVRMVSLESLPGTTVTQGSPAEVLEEKIDGHKKRKMI
                     IRALKKLPQGMQKILMQHYFGNKSLKEAGKKLKFSKSWSSRLHERALICLAENRELRA
                     LATC"
     gene            1830..3356
                     /gene="hutH"
                     /locus_tag="VJC18_11665"
     CDS             1830..3356
                     /gene="hutH"
                     /locus_tag="VJC18_11665"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HLD46081.1"
                     /translation="MRKKQSVFRYGQDHLTPFLAVKIASGEIKGIVTEPVKKRILRCQ
                     KTVLAIVEKGVSVYGINTGFGPLCKTAISKEQTKKLQENILRSHSVGVGALIDKRLAK
                     LMLVLKVHSLSFGYSGVSLETLNRIIWHIEADAIPTVPCQGSVGASGDLAPLAHLFLP
                     LIGLGQVHYQGQIISTRALFKQKGLDKIKLGPKEGLALINGTQFMAAHGVDVVVRLHN
                     ALESADIIGALSLEAMLGSSRPFSDKLLALRPFAGARHVAFRLSQLLNNSPLVKSHAG
                     CHRVQDPYSLRCMPQVHGASRDAWAYINQQIVTELNSVTDNPVIFSEKETISGGNFHG
                     QPIALPLDHASLAASELGSISERRTYLLLEGKEGLPLLLMKDTGINSGFMIPQYTAAA
                     LVSENKSLCFPASADSIPTSVGQEDHVSMGSISARKACAIINNLEYILAVELLCAAQA
                     LDYRRPLTTTPVLEACHAQVRSKITHAEHDRIFAQDLEVARQMLAAGELVIAANGIKI
                     "
     gene            complement(3379..3903)
                     /locus_tag="VJC18_11670"
     CDS             complement(3379..3903)
                     /locus_tag="VJC18_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46082.1"
                     /translation="MKTHQKLTSSCLPFLFLFLVLRFAFLLPLPASAQEPPEEEHLAV
                     ECSQRCVWLDKKCGHFFEDEDNVAVNKEVCKVIFGGKKSYCASLCKLDSGLFDNCEVP
                     SDEYRDTDDCLDDCDHHNAMCQINCLDQYKQNDQVEACALACRTTFSDDHSFCRVTCD
                     TDINAFVTCDLGHL"
BASE COUNT         1053 a          927 c         1018 g         1054 t
ORIGIN      
        1 cattgcgttg acggtgcttc gtctttgcga gaaggcatga accgtttaca ggttcatgcc
       61 gacaaagcaa tccagttcca gcgtatcatt ttctggattg tcacgccctg agcgaagtcg
      121 aagggctcgc aatgacggga attctggatt gccacgccct gagcgaagtc gaagggctcg
      181 caatgacggg aatgactcgc attgatgcga tttttcaaaa attttcagaa aaaggcaaat
      241 catcttcatt caggcgtgat tcagccagct gaacatcttg gatgtctttt ttgttgataa
      301 aataattcat gagcgccgca cgtatcactt caaccaaggt agatttttgg tttcgggctt
      361 cctgctggat agtgttcagc aaaaaatcat cgattctgat tgataataat ttcatctgta
      421 ggcctccctt cgatgcaaaa ttgacagaat atgaatgctg tttgataaat catcaaccgt
      481 atagagcacc ctatattcac caacccgtat acgatacaca gactgtcttc cctgtaattt
      541 tttcactccg tgaggcatgg ggtttatcac caaatcagaa agtgccttcc ggattttacc
      601 cttaatcgaa gctggtattt ttcgtgattc cttaactgcc gacggcttga tatagagctt
      661 aaaattcatg acaacacaat tgtattacat tgttgtttca ttggcaatgg tttatttaaa
      721 gccattgttg tttttttctg gcacctaatc aattttggca cctaatcaat tggtcaatta
      781 tttcaactgt ttcaactttc aattgtcaat tgaagtagtt gatcattgaa acaattgatg
      841 taattgtgtg gaaaggtacc tgaaaggtac ctggcacctg taatgtacct ggcacctagc
      901 gtcgataaag tacctggcac ctggctggca cctggaaccc gtcctgaaaa tcatttccct
      961 ccggctatgt tgttccatag tcatttggct atgaaacgat ggttctcaaa aagagaaatc
     1021 tgatttaatc aatcatatca atcctataga aaaactgacg ttttggcatg catgttgctc
     1081 agtgaatggt cgtcaataag caataaggag cattcatgca tacagaaaaa gaagaaaagg
     1141 tattagacaa tttatcgaca gcggaaatgg tagcgcgaaa aatgacgggt aaatatggtg
     1201 gcgcctattt tgacgatgtt aaaagttatg cgcactttgg actcattttg gcggccaggt
     1261 ctttcgatga caatcgtggt tgcagttttc agacctatgc gacgcactgt atgaaagggg
     1321 ccatcattga tggccttcgt ggcgagaagg tgatccgtcg catacgtagc aaggtgttgt
     1381 tacagaaaat tgaggacagg cagaaaaatg tgttggatca gctttcggtc aagcccggca
     1441 cgtcaatcat gaacgatatt tatgtagagc acagcaccat gttggatgtt cggatggttt
     1501 ctctggaatc gttgccgggg acgactgtga cacaggggag ccctgctgag gttctcgagg
     1561 aaaaaataga tgggcacaaa aaaagaaaaa tgatcattcg ggccctcaaa aaattgccgc
     1621 agggcatgca gaaaattctc atgcagcatt attttggaaa caaatcactc aaggaagctg
     1681 ggaaaaaact caaattttcc aaatcgtggt cttcacgtct gcacgaaagg gccctcattt
     1741 gcctggccga aaatcgggaa ctcagggcgt tggccacgtg ctaatttatt ttgttgccga
     1801 tgttcgatga ttatcattaa tgaactccta tgaggaaaaa acaatctgtt tttcgttacg
     1861 gacaagacca cttgacgcct tttctcgcag tcaagatcgc tagtggtgaa atcaaaggca
     1921 tcgtcacaga gcccgttaaa aaaagaattc ttcgctgtca aaaaaccgtg ctcgctattg
     1981 tggaaaaggg tgtctcggtg tacgggatca acacgggctt tggcccgctg tgcaagaccg
     2041 ccatcagcaa ggaacagaca aaaaaattac aggagaacat cctgcgctct cattctgttg
     2101 gcgtgggcgc ccttatcgac aaacgccttg ccaaactcat gctggtactc aaggtgcact
     2161 cgctgagttt tggttattcg ggtgtgtcgc tcgaaacgct caaccgcatc atctggcaca
     2221 tcgaggccga tgccatcccc accgtaccct gtcagggatc ggtgggggcc tcgggagatc
     2281 tggccccctt ggcgcatttg tttttaccat tgatcgggtt gggccaggtt cattaccagg
     2341 gccaaatcat ttcgacacgt gccttgttta aacaaaaagg actggacaaa atcaagttgg
     2401 gtcccaaaga agggctggcg ctgatcaatg gcacgcagtt tatggccgcg catggtgtgg
     2461 acgtcgttgt caggttacac aatgccctgg agtctgctga catcataggc gcactcagtc
     2521 tggaggccat gctggggtcg agtcgtcctt tttccgataa gctacttgcc ttgcgaccgt
     2581 tcgcgggtgc gcgtcatgtg gcctttcgct tgtcgcagtt gttaaataat tcccctttgg
     2641 tcaagtcgca cgcgggctgt catcgtgtgc aggacccgta ttcattgcgt tgcatgcctc
     2701 aagtgcatgg cgcgtcacgc gacgcgtggg cgtacatcaa tcagcagatc gtgaccgaac
     2761 tcaattccgt gacggataac ccggtgattt tcagcgaaaa agaaaccatc agcgggggca
     2821 atttccacgg gcaacccatc gctctcccgc tggatcacgc cagcctcgcg gccagtgagc
     2881 tcgggagcat ttcggagaga aggacttatt tattgctcga aggcaaggaa ggtctgccgc
     2941 ttttgctcat gaaggacacg gggatcaatt ccgggttcat gatcccgcag tacaccgccg
     3001 cggcgcttgt gagcgagaac aaatcgcttt gtttcccggc gtccgccgac agcatcccca
     3061 cttcggtggg acaggaagat catgtgagca tggggtccat cagcgcgcgc aaggcgtgcg
     3121 ccatcatcaa caatctcgaa tatattctgg cagtggaact gctctgcgcc gctcaggcgc
     3181 tcgattaccg ccgtccactc acgaccacgc ctgttctgga ggcgtgtcat gcgcaggtac
     3241 gatccaaaat tacgcacgct gaacacgatc ggattttcgc gcaggatttg gaggtggcga
     3301 ggcagatgct tgccgcaggt gagctggtca tcgcggcaaa tggtatcaag atttgatgcc
     3361 ttttattaga ggtccccttt agagatgccc taaatcacaa gtcacaaagg cgttaatatc
     3421 ggtatcacag gtgactctgc aaaaggagtg gtcgtccgaa aaggtggtgc gacaggccag
     3481 ggcgcaggcc tctacctgat cgttttgttt gtattgatcc agacaattga tctgacacat
     3541 ggcgttgtgg tgatcgcagt cgtccaaaca atcatccgtg tcgcggtatt cgtcggaggg
     3601 gacttcgcaa ttgtcaaaaa gaccagaatc gagtttgcat agcgaggcac aataactttt
     3661 tttgccgcca aaaatgacct tgcagacctc tttattgaca gcgacgttgt cttcatcctc
     3721 aaaaaaatgg ccacattttt tatcgagcca aacgcatcgt tgtgaacact cgacagcgag
     3781 gtgctcttct tcgggtggct cctgtgccga cgcgggcagg ggcaataaaa aagcaaaacg
     3841 caacacaaga aagaggaata gaaagggcaa acaggatgag gtgagttttt gatgcgtttt
     3901 catggtgtct ttctcattat agcataaaag ggagaataca caatcattaa tagtgccgag
     3961 gagtaaaata atatacaata tatacgctgg gttagatggt ttacagggcc attccgtact
     4021 ggtccctgcc tgtcggcagg caggttgtct tg
//
LOCUS       DATDUN010000838         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_537126, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000838 DATDUN010000000
VERSION     DATDUN010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537126"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VJC18_11675"
     CDS             complement(<1..506)
                     /locus_tag="VJC18_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLD46083.1"
                     /translation="MSYDISFETFLQEVIDRKASDVHISQGAPVMLRVDGELVPTKHA
                     PTVILTGEDSRLLCTKYCTEEMLAELERERELDFSFFFEDKSRFRVNLFYHMGNITGA
                     FRPIPSKIPTMEALGLPQALKGLTDLPRGLILVTGPTGSGKSTTMAAMIGHINQTRGD
                     HIITIEDP"
     gene            870..2609
                     /locus_tag="VJC18_11680"
     CDS             870..2609
                     /locus_tag="VJC18_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLD46084.1"
                     /translation="MAEQALQKEQSDQAPAKKKPIAMANNMVEAFAKDLVKEKLVSPD
                     QLTLAMASADHLGIDLGQILISRGFVEEETLLKFLSEKLEIPLHQPNSEEIEVEVLRT
                     MPFHLAKKHLTLPIRKQEDGQAIVVMADPGDPAAIEDLRGYFPEGFVKCLGSAQAILE
                     FLEKHHSRTTTIETASVTMELTDSDEVETEVETKKIREMASGPKIIATVNNIIARAKK
                     EGASDIHIEPFRENCRIRYRIDGVMRERGNLPKSMHMGVVSRIKIMCSLNIAERRVPQ
                     DGRARVKLVGKPLDMRINTCPTSFGEKVVMRLFSRDSIKTIEGLGFLERDRKIFTEII
                     SRPYGIFLVTGPTGSGKSTTMYAGLMRINSPELNIMTIEDPVENELDGINQIAVSEKT
                     GLTFAKVLRASLRQDPDVIMIGEIRDAETAEIAVRAAITGHMVLSTLHTNTAAGAIDR
                     LKDIGVQPFMIASALKGVLAQRLVRRLCQSCKQEISPENEYNLPIKKNYIAKGCDDCN
                     FTGYAPGRIGIFELIPINTDIRKLIHNNADSNEIMKYMRNNNLPSITEDGITKIEMGL
                     ISLEEVIRVIAED"
BASE COUNT          712 a          618 c          691 g          614 t
ORIGIN      
        1 attgggtctt cgatggtgat gatgtggtct ccgcgtgttt gattgatgtg accgatcatc
       61 gcggccatcg tggtggattt accggaaccc gtgggccctg tgaccaagat gaggccgcga
      121 gggagatcgg taagcccctt aagcgcctgc ggcagaccca atgcctccat cgtggggatc
      181 ttggagggga tgggcctgaa cgcgcctgtg atattgccca tgtgataaaa cagattgacc
      241 ctgaaacgcg atttgtcttc gaaaaaaaac gaaaaatcga gttcgcgctc cctttcgagc
      301 tcggccaaca tttcttcggt gcaatatttc gtgcacaaaa ggcgcgaatc ctcaccggtc
      361 agaataacag tgggcgcgtg tttggtgggc accagttcgc cgtcgacgcg caacatgaca
      421 ggggcccctt gcgaaatgtg cacatcgctg gccttgcggt caatcacttc ctgcagaaaa
      481 gtttcgaacg aaatatcata tgacataata gcctcctgtc atttgaagat taagcaattg
      541 ttgtgatgaa tgcaatgaat atacgatcac gtgtttacgt gttcaagtgt tcaagtgttc
      601 aggtgttcac gaggggcata aggcttaagg cgtaaggcaa aaccacagct tcaattgtct
      661 caacgagcaa ctacatcaat tgccaattgt ctgttgctca ttgaagtcgt tgtcgcaatt
      721 gattccgatg ttttacaggg agttatctgt tattttgttt ggcgtaaaaa gtgtggtcga
      781 tattaaatgg aaatattgct tggcaggtgg ggattcgtcc cgtacaatat aaggtgatgg
      841 aattttacca tcacgcagag gtgaatgaca tggctgaaca ggcattacaa aaagaacaaa
      901 gcgatcaagc tccggcaaag aaaaaaccca tagcaatggc caacaatatg gtggaagcct
      961 ttgccaagga tctggtcaag gaaaagctgg tctcaccaga ccaactcacg ctggccatgg
     1021 ccagtgccga tcatctggga atcgatttgg gccagatcct catttctcgc ggatttgttg
     1081 aagaagaaac cttgttgaag tttttgtccg agaaactgga aatccccctg catcaaccca
     1141 attcggaaga aatcgaagtt gaagtcttga ggaccatgcc cttccatctg gccaaaaaac
     1201 acctgaccct gcccattcgc aagcaggaag atggccaagc gatcgtggtg atggcggatc
     1261 ccggtgatcc cgcggcgatt gaagatctgc gggggtattt cccggagggt ttcgttaagt
     1321 gtctgggttc ggcccaggcc attctggaat ttctggaaaa acaccatagc cgcaccacca
     1381 cgatcgagac agccagtgtg acgatggagc tcacggattc cgacgaagtg gagaccgagg
     1441 tcgagaccaa aaaaattcgc gaaatggcct ctggtcccaa aatcatcgcg acagtcaata
     1501 acatcatcgc gcgcgccaaa aaagagggag ccagcgatat ccacatcgaa cccttcagag
     1561 agaattgccg gatcaggtac cgtatcgatg gtgtgatgcg tgaaaggggc aacctgccca
     1621 agagcatgca catgggtgtg gtgagccgca tcaaaatcat gtgctcgctc aatattgccg
     1681 agcggcgcgt tccacaagat ggtcgggccc gggtgaaact ggtcggcaag ccattggata
     1741 tgcgtattaa cacgtgcccc acctcgtttg gcgaaaaagt cgtgatgcgc ctcttttctc
     1801 gcgacagcat caagaccatt gagggtcttg gttttttgga aagagaccgc aaaatattta
     1861 ccgaaatcat ttccagaccg tacggaattt ttttggtcac aggtcccaca ggttccggta
     1921 aatccaccac gatgtatgcg ggactcatgc gtatcaattc tcccgaactc aacatcatga
     1981 cgatcgaaga tccggtggaa aatgaactcg atgggatcaa ccagatcgca gtgagcgaaa
     2041 agacagggtt aacctttgcc aaggtcttga gagcctccct gcgccaagac ccggatgtga
     2101 tcatgatcgg tgaaatccgg gacgcagaga ctgcggaaat tgccgtgaga gcggccatca
     2161 ccggtcatat ggtgttatcc accctgcaca ccaacacagc ggctggtgcg attgaccgtc
     2221 tcaaggatat tggagtgcaa ccatttatga tcgccagcgc cttaaaaggg gttctggccc
     2281 agcgtctggt gcggcgcctt tgtcaaagtt gcaaacaaga aatcagtccg gagaatgaat
     2341 acaaccttcc catcaagaag aattatatcg ccaaaggttg cgatgattgc aattttacgg
     2401 ggtacgcacc gggacgaatt ggtatttttg agctcatccc catcaatacc gacatcagaa
     2461 agttgattca taacaatgct gattcgaatg agatcatgaa gtacatgcgt aacaacaatt
     2521 tgccgtcgat cacggaggat ggtattacca agattgagat ggggctcatc agtctcgaag
     2581 aggtcattcg cgtcattgcc gaggattaat cagggtaaaa tattatgcca aagtt
//
LOCUS       DATDUN010000839         1089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_121689, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000839 DATDUN010000000
VERSION     DATDUN010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121689"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1089)
                     /locus_tag="VJC18_11685"
     CDS             complement(<1..>1089)
                     /locus_tag="VJC18_11685"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HLD46085.1"
                     /translation="GYSTPSGTTYNAYLINDTRPTLIDTVKEEFFSEMLERIREVMDP
                     AGIVSIISNHTEMDHSGSIRKMLEVCPKAEVICSPKGAEGLLRHFKKTWNLRVVKTGD
                     VLDTGGRPLQFVLMPMVHWPDSMATYAPNDHILFSNDAFGQHHASHERFADEINLSMI
                     YREAAKYYGNIVMPYGKQVQAVLKSVATLPIDQIFPSHGLMWRRTEDIQKIVALYDGW
                     SRHQTQKKVVIVFDSMWHSTERMAERLCQSFKDARIPVVFLPLQHTHISDVVTEILEA
                     RLVLFGTPILNNRMLPSMAALMMYLKGLKPTGRLAFTFGSYGWSLVGFKELENSVIEA
                     GFTLAAEGRYINYVPDEVEWEQLSQDVAG"
BASE COUNT          279 a          291 c          265 g          254 t
ORIGIN      
        1 ccccgccaca tcctgcgaga gctgttccca ttcgacctca tccggcacat aattgatgta
       61 ccggccctcg gcggccaacg taaatccggc ctcgataaca gagttttcca attctttaaa
      121 accaaccagg gaccaaccat acgaaccaaa cgtaaaggcc aaacgacccg tcggtttgag
      181 tcccttcagg tacatcatga gtgccgccat gctgggcaac atgcggttat tgaggatggg
      241 cgtgccaaaa agcaccagtc gcgcctccag aatttcggtc accacatcgg aaatgtgtgt
      301 gtgttgcaac ggtagaaaca caacgggaat ccgggcatcc ttgaatgatt gacaaagccg
      361 ttccgccata cgctcagtag agtgccacat ggagtcaaaa acaatcacga cttttttttg
      421 tgtctgatga cgggaccaac catcatacaa ggcaacaatt ttttgaatat cctcggtgcg
      481 acgccacatg agtccatgag acgggaaaat ctgatcgatg ggcaaggtgg ccacgctctt
      541 caaaacagcc tgcacctgtt tgccataagg cataacgata ttgccataat actttgccgc
      601 ctcgcgataa atcatgctca aattgatttc gtcggcaaaa cgttcatgac tggcgtgatg
      661 ctggccaaag gcatcgttgg aaaaaaggat gtggtcgttt ggcgcatagg tggccatgga
      721 atctggccag tgtaccatcg gcatgagcac aaactgcagc ggtcgcccac ccgtatccaa
      781 tacatcgccc gtcttgacca cgcgcagatt ccaggttttt ttaaaatgcc tcaaaagccc
      841 ttctgccccc ttgggcgaac aaatcacctc ggccttggga caaacttcca gcatttttct
      901 gatggagccg gaatgatcca tttcggtatg atttgagatg atgctgacaa tgcccgcagg
      961 gtccatgacc tccctgatgc gctccagcat ttcgctgaaa aattcctctt tgaccgtatc
     1021 gataagggtc gggcgggtat cgttgatgag ataggcattg taggttgttc ccgagggcgt
     1081 ggaataccc
//
LOCUS       DATDUN010000840         1497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_578909, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000840 DATDUN010000000
VERSION     DATDUN010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578909"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VJC18_11690"
     CDS             complement(<1..1161)
                     /locus_tag="VJC18_11690"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HLD46086.1"
                     /translation="MLRYFCYEEITILHRRIHADVVLVYTPPIGGFFMPFAMQIPFAL
                     TYDDVLIVPRRSTLSSRSDAQTKTRLTRQLDINIPIVTANMDSVTGSDMAIAMARLGG
                     IGILHRFNTIEENTEEVKRVKRAQNFIIEDPYTIEQDQTVAAAKEYVADIGITGLLVA
                     SPDKKLLGILSRRDFLYAPNGDTRVSQIMTPREKLVVGNKETTFEDARAIFARHKIEK
                     LPLVDEENTIIGLITSDDMKLSVDFPDANLDTDGRLIVGAAVGVHGDFVERAKAVVTA
                     GADVLVIDIAHGHSDLMINAIAALKQAVPGTPLIAGNIATAQAAKDLCDAGVDALKVG
                     VGPGSICITRLVTGCGMPQLTAVSEVAKVAKTYGVPVIADGGIKTSGDIVKAI"
BASE COUNT          389 a          374 c          331 g          403 t
ORIGIN      
        1 aattgctttc acaatatcac cagacgtttt gatgccaccg tctgcgatca ccggaacgcc
       61 gtatgttttt gccacttttg cgacttcact cacggcagtg agctgtggca tgccgcaacc
      121 ggtgaccaaa cgagtaatac aaattgatcc aggacccacg ccaactttca atgcatcaac
      181 acctgcgtca cacaaatctt ttgccgcctg cgcagttgcg atatttccgg cgatgagtgg
      241 ggtgcctggc acggcttgtt ttaatgctgc aatggcattg atcatgagat cagagtgtcc
      301 gtgcgcgata tcaatcacga gaacatctgc gcccgctgtc acaacagctt ttgctcgctc
      361 gacaaaatcc ccatgcacgc ccacggcagc accgacaatc aatcgtccgt ctgtatcaag
      421 atttgcatca ggaaaatcta cactaagttt catatcatca ctcgtaataa gtccgataat
      481 ggtattttct tcatcaacaa gtggaagttt ttctatttta tgccgtgcaa atattgctcg
      541 tgcatcttca aatgttgttt ctttatttcc aacaacaagt ttttctcttg gcgtcataat
      601 ctgtgataca cgtgtatcac catttggtgc atagagaaaa tctcgtcgtg ataaaatgcc
      661 aagcaatttt ttatccggac ttgcgacgag cagaccggtg atgccaatat ctgcgacata
      721 ttcttttgca gcagccaccg tttggtcttg ttcaatcgtg tagggatctt caataataaa
      781 attctgcgcg cgtttgacgc gcttcacttc ttcagtattt tcttcaatcg tattgaatcg
      841 atgtaatatc ccaataccac cgaggcgtgc catggcaatc gccatgtctg atcctgtcac
      901 agaatccatg tttgctgtga caataggtat gttgatatca agctgacgcg tcaatcttgt
      961 ttttgtctgt gcatcgctgc gagaactcaa tgtagaacga cgtgggacga tgagaacatc
     1021 atcgtatgtg agggcaaatg ggatctgcat agcaaaaggc ataaaaaaac caccaattgg
     1081 cggcgtgtat actaaaacga catctgcatg aatcctcctg tgtaggatcg tgatttcttc
     1141 atagcagaag tatcgtagca tgtttctttt ttgtgggcaa atactcgacg agaaaagtta
     1201 tccgcagtat actgatgtgc agagcagcca caaaaggagg tcgctcatgc ccgacaatgc
     1261 acaaagcagt gccaatggcg atacccttag agggaatccc tacatcgccc gtgaacatgt
     1321 catcctaaac cctacagatc ccaactacgg aggacggccc atcccgagga tctgcgatca
     1381 ttgcgtcttc cagcgccgtg tgggtacaac caagtactct ggctgctggg tggcccacca
     1441 aaggccgctg acggctttcc agatcacgag aggccctatg cctgaacaga ttgatgg
//
LOCUS       DATDUN010000841         4840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_412606, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000841 DATDUN010000000
VERSION     DATDUN010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412606"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="VJC18_11695"
     CDS             <1..1754
                     /locus_tag="VJC18_11695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLD46087.1"
                     /translation="KRILPHISADPEYVEMLVDEAKIAVNFNHGNIAQIYDLGRVDDD
                     YFIVMEYVDGKTFSQIIKRLNQLGKKIPLDILLYCFTELCRGLSYIHNKKGSDDRNLG
                     VVHRDVSPQNIILSYAGVIKIIDFGVAKAKVKEGRTESGVLKGKFAYMSPEQARSDVV
                     DFRSDIFSVGTLLWEMTTGERLFKRKSNHLTIQAIQKAKFDPADTRRSDVPRELDKII
                     KKALQRNPRHRYQDGSELSRELEKLLFKINPDFKPVFAAEFIYKLFGPEEDEKDLPDH
                     LFSKEVTPVTQAQHLIPQASLHKSFEQQDDPTVKDILDHTTPIVHIPVSRRMSISYLW
                     IVLLFLFITVCGSIYVYLVNKDKFGSISLTGWDDQMDVIVDNKRLLQIPTKIKLSADE
                     THKLVVKKKNFKELTFNVILKPYQHRDLQIQMTALQEESGTLFIITSPPGATVYVNNV
                     EWASKSPVIIRNLDVAQTYKVGLFLEKYQFYRKSVSFDDTGEVRLEHNFTMNFAYLSV
                     KSNPLGAEVWLDGVMMGRTPYQNNNLLPGRSYLVNIKKEGFLDDTLTVNLVPGEEKKL
                     TFTLQGQADASKETVNE"
     gene            1773..2666
                     /locus_tag="VJC18_11700"
     CDS             1773..2666
                     /locus_tag="VJC18_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLD46088.1"
                     /translation="MDETIVSSIDELNAATQAEPYLIIVAGMHIGKTYSLAEGEIIAG
                     RSPDCDIWIEDNTISRKHFRIRKSEGQCTIQDMNSTNGTFVNSKRLTKPVTLQASDKI
                     QISKDTIIQFDYFDENRKISEHKRYELGVTDPTTGAYNKSYFLQRISDEFSFGTRQQL
                     PMTILMIDIDFFKHINDQHGHLAGDKVLQEVGSVIKKTIRTEDVFCRYGGEEFVIIMR
                     NTGDQAAINLAERLRKKIEANEIIYDDETIKVTVSIGAATSHDGSFRDYVSQIKAADD
                     FLYQAKGSGRNRVCGNCKPKE"
     gene            2860..4191
                     /gene="tilS"
                     /locus_tag="VJC18_11705"
     CDS             2860..4191
                     /gene="tilS"
                     /locus_tag="VJC18_11705"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLD46089.1"
                     /translation="MFPPFLNSSDLDLIKRAPPVLLVSGGADSVFLFHQFLTLKIQAG
                     IKFRVLHFNHHLRAAESDGDQQFVRQLCARHRIPLTVIHLRNLPSSHVQETARHRRQY
                     HLFRLHQQRGWNVFVTAHHQDDVLETLFMKIGRGAGIKGLRGIPRQQIIKKGRDEICL
                     YRPLIDMSKKEILSWLKGQRAPFCTDSSNLKPVYLRNAVRLGVLAQWHDETARNRVLA
                     SVKLIQVIDDYFHKRRLFLNKRYPSSVPLAVWETWPQELQFRFFRDKMAKNGFTQQVE
                     YRHYLKIVTQDTIKLTLGEAVLLKDASGCYFFNRSQQGSRSMDSLEVQTKGAYFWGRH
                     ALCLAISLLCSSPSGDEYRMTCRCSGKPQSPLRLIINADDVPLRVSLASPSDLFKPFG
                     HKTAKPLKKFLVSRHIHRFERLFWPVLRNKQNQILAVLGLEVGEDFRASTV"
     gene            complement(4211..4468)
                     /locus_tag="VJC18_11710"
     CDS             complement(4211..4468)
                     /locus_tag="VJC18_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46090.1"
                     /translation="MPKKTKHIKSKKSKKTTAKKSVKIDPINSIEHYNAILLEEMRSE
                     FKFVIEHTSDIEKRLNARMDEHMQRINKTCANISGFLPPQE"
     gene            4631..>4840
                     /locus_tag="VJC18_11715"
     CDS             4631..>4840
                     /locus_tag="VJC18_11715"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003783959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HLD46091.1"
                     /translation="MNIRIGIGYDVHRLVPGRPCVIGGVVFDHDKGLLGHSDADVLLH
                     AICDALLGALALGDIGKLFPDTDPQY"
BASE COUNT         1294 a         1150 c         1133 g         1263 t
ORIGIN      
        1 tcaagcgcat cctcccgcac atctccgccg atcccgaata cgtcgaaatg ttggtcgatg
       61 aagccaagat cgccgtcaat ttcaatcacg gcaacatcgc ccagatctac gatctggggc
      121 gcgtcgacga tgactatttc atcgtcatgg agtatgtcga cggcaaaaca ttttcccaaa
      181 tcatcaagcg gctcaatcag ctcggcaaaa aaattccact cgacattttg ctgtattgtt
      241 ttaccgaact ttgtcgtggc ttgagctaca tccacaacaa aaaaggatcc gatgatcgca
      301 atttgggtgt ggtgcaccga gacgtctcac cgcagaatat cattctgtcc tatgccggcg
      361 tgatcaaaat catcgatttt ggcgtggcca aggccaaagt caaggaaggc cgcacagaat
      421 cgggcgtgct caaaggcaag ttcgcttaca tgtcacccga acaggccagg agcgatgtgg
      481 tcgactttcg ttcggatatt ttttcggtgg gcaccctgtt gtgggaaatg acaaccggcg
      541 aacgtctgtt caaaagaaaa agcaatcacc tcaccataca ggccattcaa aaagccaagt
      601 tcgatcccgc ggacacgcga cgttccgatg ttccccgaga gctggacaaa atcatcaaaa
      661 aggccctcca gcgcaatcct cgccaccggt atcaagatgg ctccgaattg tcgcgcgaac
      721 tcgaaaaact gctgttcaag atcaatcccg atttcaagcc agttttcgca gcagagttca
      781 tttacaaact ttttggtcca gaagaggatg aaaaagatct gcctgatcat ttgttctcca
      841 aagaggtcac gcctgtcacg caagcgcaac acctgattcc ccaggccagc ttgcacaaat
      901 cattcgagca acaggatgac cccacggtga aggatattct cgatcacacc actccgatcg
      961 tacacattcc cgtgagccgt agaatgtcaa tttcgtatct ctggatcgtc ctgctgtttt
     1021 tgttcatcac ggtgtgcggt tcgatttacg tctacctggt caacaaggat aaatttggat
     1081 ccatcagttt gacgggttgg gacgaccaga tggatgtgat tgtcgacaac aaacggttgc
     1141 tccaaatccc caccaaaatc aagttatcgg cggacgagac gcacaaactt gttgtcaaga
     1201 aaaagaactt caaggaactg actttcaatg tgatcctcaa accttatcaa catcgggacc
     1261 ttcaaattca gatgacagcc ctgcaggaag aatcggggac tttgttcatc atcacctcgc
     1321 cgccgggtgc cacggtgtat gtcaacaatg tggaatgggc cagcaaatca cctgtcatca
     1381 tcaggaatct cgatgtcgcg caaacgtaca aggtgggcct gtttctggag aaataccagt
     1441 tttaccgaaa gagcgtttct tttgacgata caggtgaggt gcggctcgag cacaacttca
     1501 ccatgaattt tgcgtatctt tccgttaaga gcaatccttt gggtgcagag gtctggcttg
     1561 atggtgtgat gatgggacgc acaccctatc agaacaacaa tcttcttccg ggcaggtcgt
     1621 atcttgtcaa catcaaaaaa gagggttttt tggatgatac cctcacagtc aatcttgttc
     1681 ccggagaaga aaaaaaactc actttcaccc ttcaggggca ggctgatgcc agtaaagaaa
     1741 cagtgaacga atgaggagtg taatttatga ccatggacga aaccatagta tcatcaatcg
     1801 acgagctcaa tgcggcgacg caagccgaac cgtatctcat catcgtggcg gggatgcaca
     1861 ttggtaaaac ctactcactg gcggagggtg aaatcatcgc gggacgctca cccgattgtg
     1921 atatctggat cgaagacaat accatttcgc gcaagcattt tcgcatccga aaatctgagg
     1981 gacagtgtac gattcaagac atgaactcaa cgaacggtac attcgtcaac agcaagagac
     2041 tcaccaaacc ggtcaccttg caggcctcag acaagattca gatttccaaa gacacgatca
     2101 tccagttcga ctattttgac gaaaacagaa aaatatccga acacaaacgg tacgagttgg
     2161 gggtcacgga tcccacgacg ggtgcttaca acaaaagtta ttttctccag cgcatctcag
     2221 acgaattttc gttcggcaca cgtcaacaat tgcccatgac gatcctcatg atcgacatcg
     2281 actttttcaa gcacatcaat gaccagcatg gtcatctggc tggcgacaaa gttctgcagg
     2341 aagtgggctc ggtcatcaaa aagaccatca ggacggagga cgtgttctgc cggtatggcg
     2401 gagaggagtt tgtcatcatc atgcgcaaca cgggcgatca ggcagccatc aatctggccg
     2461 agagactcag aaagaagatt gaggccaacg aaattatcta tgatgacgaa accatcaagg
     2521 tcacggtctc gatcggtgcg gccacatccc acgatggcag ttttcgtgat tacgtgtcgc
     2581 agatcaaagc cgccgatgac tttttgtatc aggccaaggg gtctggccgc aatcgtgttt
     2641 gtggcaattg caaaccaaaa gagtgaacat ttttttcacc accccttatg gaggctgtcg
     2701 attcaggtga ctttcaagaa tattgtatcc cagcgaaagc agggctacat ctgactgtca
     2761 tcccagcgtc ccgcaaagcg ggatcagtcc tatggctgat aggctgggat ccattaatta
     2821 atttctggat tcctgctttc gcaggaatga caaagaatta tgtttccccc cttcctaaat
     2881 tcttccgatc tcgatctcat caagcgtgcc cccccggtac tgctggtctc cggcggagct
     2941 gattccgtgt ttctgtttca tcaattcctc acgctcaaaa tacaagcagg catcaagttt
     3001 cgagttttgc attttaatca tcatctgagg gcggcggaat cagatggtga tcagcaattt
     3061 gtcaggcaac tttgcgcgcg tcatcgcatc cctttgactg tcattcatct cagaaatctt
     3121 ccctcgtccc acgtccaaga aacagccaga cacagacgcc aataccatct tttcagattg
     3181 catcagcaaa gaggctggaa cgtatttgtg acggctcacc atcaggacga tgtgttggaa
     3241 acgctattta tgaaaattgg cagaggagcg ggcatcaagg gactaagggg tattccgcgg
     3301 caacagatca tcaaaaaagg tcgtgacgag atttgccttt atcgtcccct catcgatatg
     3361 agcaaaaaag aaattttgtc atggctcaaa ggtcagcggg cacctttttg cactgactcg
     3421 tcaaatctca aaccagtcta tttacgcaat gccgtcaggt tgggcgtgtt ggcacaatgg
     3481 cacgacgaaa cagcacgcaa tagggtgttg gcgagtgtta aactcattca agtgattgat
     3541 gactattttc ataaaagacg actgtttctc aacaaacgct acccatcatc tgtccctttg
     3601 gccgtgtggg agacgtggcc acaggaattg caatttcgat ttttcagaga caaaatggcc
     3661 aaaaacggtt ttacacaaca ggtggagtat cggcattatc tcaaaatagt cacacaggac
     3721 acaatcaaat tgacattggg cgaggccgtt ttattgaaag atgcgtcagg gtgttatttt
     3781 tttaacaggt ctcaacaggg cagtcgatca atggattcgt tggaggtgca gacaaaggga
     3841 gcctactttt ggggacggca tgctctgtgt cttgccataa gccttttgtg ttcgtcaccc
     3901 agtggcgatg aataccgaat gacctgtcgc tgttcaggaa aacctcaatc cccattgcgt
     3961 ttgataatca atgctgatga tgttcctttg cgagtcagtt tagcaagccc atcagattta
     4021 tttaaaccct ttggacacaa aacggcaaag cctctaaaaa aatttctggt gtcacgccat
     4081 atccacagat ttgagaggct tttttggcct gtgcttcgaa acaaacaaaa tcagatcctg
     4141 gcagtgttgg gattggaggt gggggaggat tttagggctt ccacagtgta aagtgactca
     4201 tgcaatattg tcattcctgc ggaggcagga atccagaaat atttgcacaa gttttattaa
     4261 ttctttgcat atgttcatcc atccgcgcgt tgagtctttt ttcgatgtcg ctggtgtgtt
     4321 ctatgacaaa tttgaactct gatctcattt cttcgagcag aatggcattg taatgttcaa
     4381 tgctgttgat gggatctatt ttgactgatt tcttagcagt tgttttttta cttttcttgg
     4441 attttatatg ttttgttttt ttgggcataa cgaacctcac gtttgacttt ttaagtctag
     4501 agtccaacct ttaggaaatg tcaatcaggt ctgtaacaaa tatctaacag cttgatacga
     4561 ttgatattcc ctattggcga ggggaaattc tgattgccct ctaaagcttg ttggtgctat
     4621 gcgatccaac atgaatatca ggattggtat aggttacgat gtccaccgtc tcgttccggg
     4681 caggccgtgt gtcatcggtg gtgttgtgtt cgaccatgat aagggattac tcgggcattc
     4741 cgatgccgat gttcttctgc acgctatttg tgatgcactt cttggggctt tggcattggg
     4801 tgatatcggc aaattgtttc ccgatactga tccccaatac
//
LOCUS       DATDUN010000842         3929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_537421, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000842 DATDUN010000000
VERSION     DATDUN010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537421"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VJC18_11720"
     CDS             <1..772
                     /locus_tag="VJC18_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLD46092.1"
                     /translation="ASVLEKDYAMKKGIKAAMLYPKIVVFVLCSAISALMILVVPKFK
                     TMYDSLNTELPLPTKIMIGTSNFFVDYGWMLLIAVIFLFVAYNKFYKTAKGKYLIDRF
                     TFKPPIFGPLALKVANARFSNILACLYKSGLPVTKALSITAETIGNEAFMREVRMLQA
                     DVEKGHGIADSMRKLTFFAPIIIEATNIGEKTGSLDTMLISMGEHYDIEISHTTKNLS
                     TMLEPILLVMIFGIVTVFALAIFLPMWGMHSAMLGKGG"
     gene            complement(897..1370)
                     /gene="greB"
                     /locus_tag="VJC18_11725"
     CDS             complement(897..1370)
                     /gene="greB"
                     /locus_tag="VJC18_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HLD46093.1"
                     /translation="MKKPNYITPSGLKNIQSELYQLLHVERPQLVKTVAWAAANGDRS
                     ENADYIYGKRRLREIDRRIRFLQSRLDHIEVIDPARVVSDRVSFGATVTVIDEEGAQA
                     AYQIVGSDETDARQKKISWESPMARALLGKKKGDAVIATVPKGKIDLEIIEVKYK"
     gene            1446..1850
                     /locus_tag="VJC18_11730"
     CDS             1446..1850
                     /locus_tag="VJC18_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46094.1"
                     /translation="MKKQAMAVLSIFVSWTIIDYVIHGLLLSDLYRQTAQLWRPMPEM
                     NMGLMYAVTMVGALVLVLMFDRFVQTKNKNTAMQFGFLFGLSAGTSMGFGSFSVMPIP
                     FVMAFVWFLGVVVQGTVAGMLLALTMGDEKRV"
     gene            complement(1941..2462)
                     /locus_tag="VJC18_11735"
     CDS             complement(1941..2462)
                     /locus_tag="VJC18_11735"
                     /inference="COORDINATES: protein motif:HMM:NF002486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HLD46095.1"
                     /translation="MWKGQKNNMTKIAILDPCPCGSGINFENCCQPCLLGLEKPASAE
                     KLLRSRYSAFVAKNTDYIFATHHPEKIKTLEKQGIIDWANNAHWLGLEIQSVDDSPLN
                     NDETVISFIARYTQDNVTYNHRETSLFKRKDGVWYFYDVQKNTPIKLENKTGRNDPCP
                     CGSGKKFKKCCGA"
     gene            complement(2440..2997)
                     /gene="efp"
                     /locus_tag="VJC18_11740"
     CDS             complement(2440..2997)
                     /gene="efp"
                     /locus_tag="VJC18_11740"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HLD46096.1"
                     /translation="MTVATQIKKGEVLKLNNDLYRIIRLDHITPGKGNAIVHADLRSL
                     TTGVKTNRRFRSSEDVESVVVYNRKMQYLYQEAELYHFMDTETFDQYELGKNVLDDAI
                     YYILPDHKYDVMIFEEVPIGIDIPPSVTMKIIETEPSQKGITGKTKDAKLETGLSIKV
                     PLFIEAGESVVINTQTNEYVERAKK"
     gene            3173..>3929
                     /locus_tag="VJC18_11745"
     CDS             3173..>3929
                     /locus_tag="VJC18_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLD46097.1"
                     /translation="MTFKEANPQIDFAKEEEQILKFWEENRIFEKSVKDRAHKKSYTF
                     YDGPPFATGLPHYGHLLAGIIKDIIPRYWTMKGHHIERRFGWDCHGLPIEALAQDQLG
                     LAGVGAIVEKGVDVFNETCRSMVLKYTAEWRTTVRRMGRWVDFDDDYKTMDASFMETI
                     WWVFKQLWEQGRIYKSYRIMPYSHKLTTPLSNFEAGNNYQDVQDPAITIRLKIKQGDP
                     DLMSQGNAYVLIWTTTPWTLPSNLAVCVGPDITY"
BASE COUNT          919 a          896 c         1022 g         1092 t
ORIGIN      
        1 cgcgtcggtt ttggaaaaag actacgccat gaaaaaaggg atcaaggcgg ccatgttgta
       61 cccaaaaatc gtggtgttcg ttttgtgctc ggccatctca gccctgatga tcctggtcgt
      121 gcccaaattc aaaaccatgt acgattcatt gaataccgaa ttaccgttgc ccaccaaaat
      181 catgatcggg accagcaact tttttgtcga ttacgggtgg atgctgttaa tagcggtcat
      241 ctttttattt gtcgcgtata acaaattcta caaaacggcc aaagggaaat acctcattga
      301 ccgctttacc ttcaaacccc ccatttttgg tcccttggcg ctaaaggtgg ccaacgccag
      361 attttccaac attctggctt gcctgtacaa gagcggtctg cctgtgacca aggcgctcag
      421 catcactgcc gagaccattg gcaatgaggc cttcatgcgt gaagtgagga tgttgcaggc
      481 cgacgtggaa aaagggcacg ggatcgccga tagcatgcgc aaactgactt tttttgcccc
      541 catcatcatt gaggcgacaa acatcggtga gaaaacaggt tcgctggata ccatgctcat
      601 cagcatgggc gaacattacg acatcgaaat ttcacacacg accaaaaacc tctcaacgat
      661 gctggaaccg attttgctgg tcatgatctt tggtatcgtg actgtttttg ctctcgccat
      721 cttcctgccc atgtggggta tgcacagcgc catgttgggg aagggggggt gaacgatacg
      781 agctcacgag ggacgaaggg acgagatgca atcatgcaat tagcaatggg caattgcagc
      841 aattgcaatg attgcaacaa ttgcagtagt tgccatagtt gaattctatc tgttccttat
      901 ttgtatttca cttcaataat ctccaaatca atcttcccct tgggcacggt ggcgatgacc
      961 gcatcgcctt ttttcttgcc cagcaaggcc ctggccatgg gcgattccca actgattttt
     1021 ttttgtctgg catcggtttc gtcggacccg acgatctgat aggcggcttg tgccccctcc
     1081 tcatctatca cagtgactgt ggccccaaaa ctcacacggt cgctcacgac ccgcgcggga
     1141 tcaatgacct caatatgatc cagccgggac tgtaaaaaac ggatgcggcg atcgatttcg
     1201 cgtagtctgc gtttgccata gatgtaatcc gcgttttcag aacgatcgcc gttcgcggcg
     1261 gcccaggcga ccgttttaac aagctgtgga cgttcgacgt gaagcagttg atacaattcg
     1321 ctttgaatat ttttgagacc ggagggggtg atgtagtttg gttttttcat aatacgcttg
     1381 gcaaccacaa cgggttagca tattgaggat aaaaatcaag gctacggtga gccaagggag
     1441 ataatatgaa aaaacaagca atggctgtgc tcagtatttt tgtgtcgtgg accatcatcg
     1501 attatgtaat tcatgggttg ctgttgtccg atctctacag gcaaaccgct cagttgtgga
     1561 ggcccatgcc agagatgaac atggggctca tgtacgctgt caccatggtg ggtgcgttgg
     1621 tcctcgtttt gatgttcgat cgatttgtgc agaccaaaaa caagaatacc gcgatgcaat
     1681 ttggtttttt gtttggattg agcgctggca cctccatggg atttggcagt ttctccgtga
     1741 tgcccatccc ttttgtgatg gcttttgtct ggtttctggg ggttgtggtt cagggcactg
     1801 tggcgggtat gttattggcg ttgacgatgg gggatgagaa aagggtataa aagtcgctgt
     1861 caaatacttt gtgtcaaata ttgtcaattg cttcgcgtca aagggtcata aggttacgcc
     1921 gccggagatg actgttggga ttatgcccca caacacttct tgaacttctt gccactcccg
     1981 caggggcagg ggtcgttgcg gcctgtcttg ttttctaact tgatgggcgt gtttttttga
     2041 acatcgtaaa aataccaaac cccgtctttt cttttgaaaa gtgaggtttc gcggtgattg
     2101 taggtgacgt tgtcctgtgt gtaacgggcg ataaatgaaa tgacggtctc gtcattattc
     2161 aaaggggaat catccaccga ctggatctcg agccccagcc aatgggcgtt attggcccaa
     2221 tctatgattc cctgtttttc cagtgtcttg attttttcgg ggtggtgggt cgcaaaaatg
     2281 taatccgtat ttttggccac aaacgctgag taccgtgagc gtaagagttt ctcggcgctc
     2341 gcgggttttt ccagtcccaa caggcagggt tggcaacagt tttcgaagtt gatcccggag
     2401 ccgcaagggc aggggtcgag gatggcgatt tttgtcatat tatttttttg ccctttccac
     2461 atactcgttg gtttgtgtgt tgatcaccac ggattcaccc gcttcgatga aaagtggcac
     2521 tttaatcgat aaccctgttt ccagtttggc gtctttggtt ttacccgtga tgcccttttg
     2581 ggaaggttct gtttcaatga ttttcatggt gacgctggga gggatgtcga tgccaatggg
     2641 gacttcttcg aagatcatca catcgtattt gtgatctggc aggatgtagt aaattgcgtc
     2701 gtctagtacg ttttttccca actcgtactg gtcaaatgtt tcggtatcca taaaatgata
     2761 cagttctgcc tcctgataaa ggtattgcat tttgcgattg tacaccacaa ccgattccac
     2821 gtcttcggaa gagcgaaagc gtctgttggt cttgacgccg gttgtgaggc ttcgcaaatc
     2881 cgcatgcaca attgcgttgc cctttccggg agtgatgtgg tcgagcctga tgattctgta
     2941 gaggtcgttg ttgagcttaa gaacctcacc ttttttgatc tgtgtggcga ctgtcattta
     3001 agtctccgtg gtttaatgtg tggacaacca atgccgacgc caagcatagc aagtaacatg
     3061 agattattca aaagtatttt tgacatatcg ggaggggagt attatttgtc tacgctcact
     3121 tgataagttt ggggattctc tggttttcag cgtttccctg caggagcgcc gcatgacctt
     3181 taaagaagcc aacccccaga tcgattttgc caaggaagaa gaacagattc tcaaattctg
     3241 ggaagagaac aggatttttg aaaaaagtgt gaaggaccgt gcacacaaaa aatcatatac
     3301 tttttatgat gggccaccct ttgccacggg actgcctcac tacgggcatc tgctggccgg
     3361 gatcatcaag gatatcatcc cccgttactg gaccatgaag gggcatcaca tcgagaggcg
     3421 ctttggttgg gattgtcacg gattgcccat tgaggctctg gcacaggatc agcttggttt
     3481 ggcgggagtg ggggcgattg tcgaaaaagg cgtggacgtg tttaacgaga cctgccggtc
     3541 catggtgctc aaatacacgg cagagtggcg cacaacggtg aggcggatgg gacgctgggt
     3601 tgattttgat gacgattata aaaccatgga cgcgagtttc atggagacca tctggtgggt
     3661 attcaaacag ctttgggaac agggacgcat ctacaaatct taccgcatca tgccctacag
     3721 tcacaagctc acgaccccgc tttcaaattt tgaggcgggc aacaattatc aggatgtgca
     3781 agaccccgcg atcacgatcc ggttgaaaat caaacagggt gatcccgatc ttatgtctca
     3841 aggcaacgcc tatgttctca tctggacaac cacaccgtgg accctcccgt ccaatctggc
     3901 tgtgtgcgtt gggccggaca tcacatatt
//
LOCUS       DATDUN010000843         1294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_579277, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000843 DATDUN010000000
VERSION     DATDUN010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579277"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VJC18_11750"
     CDS             <1..379
                     /locus_tag="VJC18_11750"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HLD46098.1"
                     /translation="IAAVALGASVIEKHIKLNNSEETVDSFFSLTVAEFKTMAGEIRN
                     AEKAIGRVNYDLTKGALLNKHGRRSLYVSAPIKKGQIFSADNIKSVRPGYGLEPKYKP
                     IVLGRKATKDLDIGDRLSWDVIE"
     gene            391..651
                     /locus_tag="VJC18_11755"
     CDS             391..651
                     /locus_tag="VJC18_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46099.1"
                     /translation="MKDHLKTEIHEFITNYIHNYAAGENLVLDGSTNFVADRLLDSFA
                     ILNLIMTLESQYAVKFQAKELADPRLQTIEALAQIIFEKSTI"
     gene            664..>1294
                     /locus_tag="VJC18_11760"
     CDS             664..>1294
                     /locus_tag="VJC18_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HLD46100.1"
                     /translation="MGILFNTFREAAQRFPNKTALVTQSGAHSYASLLLQATKWAALL
                     HTRHGIHQPRVALLTENPFHTITASFGIARLDGNCVPTNTQLHAEQQLANWQATDVEI
                     VIYEPAFEHKIKANSKSGLIFISTHEISSIDASIEIPATFDWSDRNEFLIAFSSGSTG
                     TPKPTILSQQNKLLRAQQSWDLYDLTSDDVVLCASPYFHSLGQRLVYVAL"
BASE COUNT          361 a          345 c          276 g          312 t
ORIGIN      
        1 tattgctgca gttgctttgg gcgcatctgt gattgaaaag catataaagc tcaacaacag
       61 cgaggaaacc gtagacagtt ttttttctct cactgtcgca gaattcaaaa ccatggcggg
      121 cgaaatccgc aatgcggaaa aagcgatcgg tcgcgtaaac tatgacctga ccaaaggggc
      181 cttgctcaac aaacacggac gcagatcact ctacgtcagc gctccaataa agaagggcca
      241 gatttttagc gcagacaaca ttaagtccgt acgacctgga tatggattag aacccaaata
      301 caagcccatc gtcttgggac gcaaggcgac aaaagactta gacattggcg atcgccttag
      361 ttgggatgta atcgagtaaa tgaggacgca atgaaagacc atctgaaaac tgagattcat
      421 gaattcatta cgaattacat tcacaattac gcagcagggg aaaatcttgt cctcgacgga
      481 tcaaccaatt ttgttgcgga tcgcctactc gattcatttg ccattctgaa cctgatcatg
      541 acgctggaaa gtcaatacgc cgtgaagttc caggccaagg aactggcgga cccccgtcta
      601 cagaccattg aagcgcttgc acaaattatt tttgaaaaaa gcaccatata agcaagggac
      661 ctcatgggta ttttattcaa caccttccgg gaagccgccc agcgttttcc caacaaaaca
      721 gcccttgtca cccaaagtgg tgcccacagt tatgcctctc ttttgttgca agcaacgaag
      781 tgggctgcgc ttttacatac acgccacggc attcatcagc cgcgtgttgc gcttctgact
      841 gaaaatccgt ttcacacgat cactgcctcc tttggcattg cgcgcctgga cggcaattgc
      901 gtccccacaa acacgcaact gcatgccgag cagcagcttg ccaactggca agccacagat
      961 gtcgaaatcg ttatttacga acccgctttt gaacacaaaa ttaaagccaa ttccaaatct
     1021 ggcttgatct tcatttccac ccatgaaatt tcctcaatcg atgcatcgat tgagatccca
     1081 gccaccttcg actggtctga tcgcaacgaa ttcctgatcg ctttttcttc cggatcaacg
     1141 ggcacgccaa aaccaaccat tttgtcacaa caaaacaagt tattgcgtgc gcaacagtcg
     1201 tgggacttat acgacctaac gtctgacgac gtggtgttat gcgcctctcc ctactttcac
     1261 tcattgggtc aacgcttggt gtatgtcgca ctcc
//
LOCUS       DATDUN010000844         1146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_579376, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000844 DATDUN010000000
VERSION     DATDUN010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579376"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VJC18_11765"
     CDS             <1..182
                     /locus_tag="VJC18_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46101.1"
                     /translation="ILMGDPLQVDNPLCSRSINGLTHAIKHYLGKPYASLVTLPHNYR
                     SQMSQDTDDWKVYSA"
     gene            complement(188..>1146)
                     /locus_tag="VJC18_11770"
     CDS             complement(188..>1146)
                     /locus_tag="VJC18_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46102.1"
                     /translation="ARHAKRYVAGPAPLLEIGAGNRLVPMGLKDLFGDALHITELAPR
                     YSSTKRGVDRELPAATIDRADLPRSAFELAYSFFGTYYSNRQLVVLQKLADSLKVGGE
                     FFGFMKPNGTNRRLMELVARWPMIFYKGGLELVIEDHYFGTPPIGEPALVVSARKCAE
                     SIDVGQLFDQARRADKKGGEHEHLSLVSDPSIRLTLDGPRTALSQAEELLPQLIQQVV
                     RWFCQTYKKNPAQLYYQMTGERLVLQSADEPERETLGMQAIVDDLLSLDGLQKLICGV
                     PLTTMLMRHFYPTAKLTTSENVVDTRCAATESLCEWLSALHQ"
BASE COUNT          287 a          340 c          251 g          268 t
ORIGIN      
        1 tgatccttat gggagatccc ctgcaggtgg acaacccgct ttgttcgcgt tcgatcaacg
       61 ggctcacgca cgccatcaaa cattatctgg gcaagcccta cgcatcgctc gtgaccctgc
      121 cgcacaacta ccgcagtcag atgtcacagg atacggatga ttggaaggtt tacagcgcgt
      181 aggaactcta ctggtgcaat gcggacagcc attcacacag actttcggtc gcggcgcatc
      241 gcgtgtctac aacattttct gaggtggtca gtttggcagt tgggtaaaaa tgtctcatca
      301 acatcgtggt caagggcaca ccgcaaatca atttctgcag accatcgagg ctcaataaat
      361 catcaactat cgcctgcatc cccagggttt ctctttctgg ttcgtctgca ctctgcaaaa
      421 caagtctttc ccccgtcatt tgataataca actgagcagg gttttttttg tacgtctggc
      481 agaaccaacg aaccacctgc tgtatcagct gaggcaaaag ctcctcagcc tggcttagcg
      541 ccgtgcgagg accatccaaa gtcaacctga tgctcgggtc actgaccagg gataggtgtt
      601 catgctcccc gcctttcttg tctgcgcgac gtgcctgatc aaacaattgc cccacatcaa
      661 tgctctccgc acacttgcga gccgacacaa ccaaagccgg ttctccgata ggaggtgtcc
      721 caaaataatg gtcctcaatc accaattcca agccaccttt gtaaaagatc atgggccaac
      781 gcgcaaccaa ttccatcaat cgcctgttcg taccattggg tttcatgaat ccaaaaaatt
      841 ctcccccaac cttcaggcta tccgccagct tttgcaaaac aacaagttgc cgattggaat
      901 aataggtccc aaaaaatgaa tacgccagct caaatgcact gcggggcaaa tccgccctat
      961 ctatcgtcgc cgcaggcaac tcccggtcaa cccctctctt ggtagaggaa taccgcggtg
     1021 ccaattccgt tatgtgcagg gcatcaccaa aaaggtcttt taaccccatg gggactaatc
     1081 gattgcccgc acctatttcc aacaatggcg cagggccagc aacgtacctc ttggcatgtc
     1141 ttgcca
//
LOCUS       DATDUN010000845         1409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_122055, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000845 DATDUN010000000
VERSION     DATDUN010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122055"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..235
                     /locus_tag="VJC18_11775"
     CDS             5..235
                     /locus_tag="VJC18_11775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HLD46103.1"
                     /translation="MGTKPLSPLISQWVTTGTQIFSLVIKTSFFCWLFIWIRWTFPRV
                     RYDQLMTLGWKFLLPLGMINLVITVIRVMGNG"
     gene            320..823
                     /locus_tag="VJC18_11780"
     CDS             320..823
                     /locus_tag="VJC18_11780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HLD46104.1"
                     /translation="MHIWLFYIFSTITVAATILMIAHKNTITSAMSLVVSLCSLAALF
                     LLLHAPFVAAVQIIVYAGAIMVLFIFVIMMLQIPKPHQKTTRFGIKHLLVGSCGCMLF
                     AFFFVAFATIPQVSFKTVTADFGSAQQVGETLFSKYVIPFELLGVLLLSGLVGALFLG
                     RKEEEIK"
     gene            827..1135
                     /gene="nuoK"
                     /locus_tag="VJC18_11785"
     CDS             827..1135
                     /gene="nuoK"
                     /locus_tag="VJC18_11785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HLD46105.1"
                     /translation="MREITIHHYIILSSLLFCLGFVGIILRRNLLVVLMCLEVLMNAI
                     ILAFVAFARYYNEHDGHVMAMIIMAIAAVEAAIGLGIVVTIFHHRKSIDTNDLKSMKG
                     "
     gene            1159..1368
                     /locus_tag="VJC18_11790"
     CDS             1159..1368
                     /locus_tag="VJC18_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46106.1"
                     /translation="MKISTYIILIPLIPLIGFFINGLIALQQSRGQGFVSKGTTSVIA
                     CLASLLSFILVLCAGGTLITHPATP"
BASE COUNT          308 a          337 c          339 g          425 t
ORIGIN      
        1 gtttatgggg acaaaaccgc tgtctccgct tatcagtcag tgggtcacca caggcactca
       61 aattttctca ctcgtcatca aaacctcttt tttttgttgg ctgttcattt ggatcagatg
      121 gacatttccc agggtacgct acgatcagtt gatgacgctg ggctggaaat ttttactccc
      181 attgggaatg atcaatttgg tgattacggt tattagagta atgggtaatg ggtaacggta
      241 gacgtgttca agtgttcagg tgttcacgtg agttcgtgag ttcgtgagct cgtgagcgtt
      301 gggagttggg gaatttggca tgcacatctg gttattttac atattttcca cgatcactgt
      361 ggccgcaacg attctgatga ttgcgcacaa aaacaccatc accagtgcga tgagtctggt
      421 cgtttcgctc tgttctttag cggccctctt tttgctcctg cacgcccctt tcgtggccgc
      481 tgtgcaaatc atcgtttatg cgggggccat catggttctc ttcatcttcg ttatcatgat
      541 gttacagata ccgaaaccac atcaaaaaac cacacgtttt ggcatcaagc acctcttggt
      601 ggggagttgc ggttgtatgc tgtttgcctt ttttttcgtc gccttcgcca ccatccccca
      661 ggtatccttt aagacggtca cagccgactt tggatcggcg caacaagtgg gtgaaacact
      721 tttttcaaaa tacgtcattc cgtttgaact ccttggggta ttgttattgt cggggttggt
      781 gggtgccctg tttttgggga gaaaagagga ggaaatcaag taagctatgc gtgaaatcac
      841 aatccaccac tacattatct tatcttccct gctgttttgt ctgggttttg tcggcatcat
      901 tttgcgccgg aacctgttgg ttgtgctcat gtgtcttgag gtcctcatga acgccatcat
      961 tttagccttt gtcgccttcg cccggtatta caacgaacat gatggacacg ttatggccat
     1021 gatcatcatg gccattgcgg ccgtcgaagc agccatcggc ctggggattg tggtcacgat
     1081 ttttcatcac agaaaaagca tcgacaccaa tgacctgaaa tcaatgaaag ggtaggtcta
     1141 aacaatgcaa agtttaaaat gaaaatttct acgtacatca tcctcatccc cctcatccct
     1201 ctgattggtt ttttcataaa tgggctcatt gcgctgcagc aatcccgcgg acaggggttc
     1261 gtgagcaaag gcacaacgtc tgtcatcgcc tgtctggcca gtctcctgtc atttattttg
     1321 gtcctgtgtg ccggtggcac actcatcacc catccagcga cgccatgatt gtcctggacc
     1381 agcgcctacg aatggattgc cctgggaaa
//
LOCUS       DATDUN010000846         1181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_579626, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000846 DATDUN010000000
VERSION     DATDUN010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579626"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VJC18_11795"
     CDS             complement(<1..1096)
                     /locus_tag="VJC18_11795"
                     /inference="COORDINATES: protein motif:HMM:NF020445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DndE family protein"
                     /protein_id="HLD46107.1"
                     /translation="MSDISLENVLVSNFQTSAEADKITEMLKGKLALSVRYRVARLAI
                     GRSLGEPTFPTQNVDAQGKTIKGDLLFGLDEYPLWVGMLVTNIKKHIPKPEITISALQ
                     YAVRRHWHRGILLLLDDWKEAGEEPRKFWEILIHRRAMLPDTVSKLGKFGEIHHDDEM
                     GFAGLAKAVYVDLGKNVDAPHEQYKRLVNGVGTSPHMAIMGQSGSGKTRIMKEVLKQI
                     HAQTSAPVILLDLGKGDLANDTSLIDALGAKILRVPEDGIPLDMFYASNQNDEVATDT
                     VMGFRDSFSSVVQGKLGPKQLDNLREALKPLFMAEKKITLENVRARLDQHYQDNELKV
                     DSVMSTINDLTQYTIFRPDFSPQQFFSQSWI"
BASE COUNT          293 a          293 c          265 g          330 t
ORIGIN      
        1 taatccaact ctgggagaag aattgctgtg gcgagaaatc tggcctaaag attgtgtact
       61 gtgtcaggtc gttgatagtg gacatcacgc tatccacttt taattcgttg tcctgatagt
      121 gctggtcaag ccgcgcgcgc acattttcaa gtgttatttt cttttcagcc atgaataacg
      181 gttttaatgc ttcccgaaga ttgtccaatt gttttggccc cagcttacct tgtacaacgc
      241 tggagaaaga atcacgaaaa cccatcaccg tgtcggttgc aacttcatcg ttttggttgg
      301 aggcataaaa catatccaac ggaatgccat cctctgggac gcgcaatatt ttcgcaccta
      361 atgcgtcaat caacgacgta tcgttggcta gatcaccctt gcccaaatcc agcagaatca
      421 cgggtgcgga agtttgtgca tgaatctgct tcagcacctc tttcatgatg cgggtcttac
      481 ctgaaccaga ctgccccata atcgccatgt gcggagatgt gccaacgcca ttaaccagac
      541 gtttgtattg ttcatgtggc gcatccacgt tcttacctaa atcgacatac acagccttgg
      601 ccaagccagc aaaacccatc tcgtcgtcat gatgtatctc gccaaacttg cccaatttag
      661 agacggtgtc aggcagcatg gctcggcgat gaatcaagat ttcccaaaat ttacgcggtt
      721 cttctcctgc ctctttccaa tcatccagca ggagcaggat gccacgatgc caatgtcgcc
      781 tgactgcgta ttgcagagcg gaaatcgtaa tttcgggttt cggaatgtgt tttttgatat
      841 tggtaacgag catcccaacc cacaatgggt attcgtccaa accaaacagc aaatcacctt
      901 tgattgtttt accttgggca tccacgtttt gcgtgggaaa tgtgggttca cccagtgagc
      961 gaccaatggc aagacgggcg acacgatacc gtactgaaag tgccaattta ccttttagca
     1021 tttcggttat tttgtcggct tcggcactgg tctggaagtt ggagaccagt acattttcta
     1081 agctgatgtc actcattact gacctccaaa ataggcgttt tttgtggcaa ctgtcatgcc
     1141 gacaccattg ccaatttgtt gatgctgaag caaatacgac t
//
LOCUS       DATDUN010000847         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_122215, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000847 DATDUN010000000
VERSION     DATDUN010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122215"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2092)
                     /locus_tag="VJC18_11800"
     CDS             complement(<1..2092)
                     /locus_tag="VJC18_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HLD46108.1"
                     /translation="MFFIVPVSDVYPEEVVLSDVVIAFDGPKEFKSQLNTVVPYKKGQ
                     VVNEDDLRVHFEKMRQSGLFSDITYELLSNGIGGQALKVDIHKALRVRNVNVDGNYPI
                     LAKNIKKLIALQPGTDFQPGLIPESVEAIRTYLEKNGFFESRVDIEVKREQRHDVVDL
                     LIRIHHGVTYHIDQIFVTGTHQFSEQKIRRKVSQISRFRINRLKKNLTKIKKMYARHG
                     YIKARVSLEGLVFHNDTRKVDLYLKIRENKKFKLQIEGNSFFPQRRLQQVTKLAELRS
                     YDRYAINNGKERLQRYYEQRGFPNAQIASDVIKPDKKSVIAKYSIEAGKRVALSNIHF
                     DGNDELGTKKLKKVMASQESSFWSRQYFNQDKLWKDRDHLQNEYTRWGFFEAQVAQPT
                     WTENRFGDQKSVLFQISEGPEYRLGEISVASDEPVVTGDLLEVGGLKEGKAYQEDKVE
                     EARARILDALLAEGYAYADVAVLKQLAPDAQRVNIEFSITRGPRVVVRTLVIDGHLHT
                     REAVIRKNLKIKQGENFVYQKMLDGQLNLRKLGAFASVRITPVGFEERQTGIDLVVNV
                     VERKSLVMNLQGGFDSRHWGTGEFNLTKYNLFGTGRQLNMRLIGGPKYDRGEVTFYSP
                     RVFGANWNLSTQAFGQYEDEVYYAAASYGAFASTLKNFGPHWTFGFKEQVSHTSVFES
                     ISDIAALGDSLFDNT"
BASE COUNT          540 a          602 c          492 g          571 t
ORIGIN      
        1 aggtattatc aaagagtgag tcacccaaag ccgcaatgtc ggaaatcgac tcgaacacgc
       61 tcgtgtgtga cacctgctct ttgaatccga acgtccagtg aggcccaaaa ttcttgagtg
      121 tgctggcaaa ggccccgtaa ctggccgccg catagtacac ctcatcctcg tactgaccaa
      181 aggcctgcgt cgataaattc caattggccc caaaaacacg tggcgagtaa aaggtcacct
      241 caccgcggtc atacttgggg ccaccgatca aacgcatgtt gagctgacgc cccgtgccaa
      301 acagattgta tttcgtcagg ttgaattcgc cagtccccca atgacggctg tcgaaacctc
      361 cctgaaggtt catcaccaaa ctcttgcgct ccaccacgtt caccaccaga tcaatgccgg
      421 tctgacgttc ctcaaaaccc accggtgtga tcctgaccga cgcgaaggcc cccaatttgc
      481 gcaggttcaa ctgcccatcc agcatctttt gatacacgaa attttcgccc tgtttgattt
      541 tgagattctt cctgatcacc gcttcgcgcg tgtgcagatg cccatcgatc acgagcgtgc
      601 gaaccaccac gcgtggccct ctggtgatag aaaattcgat attgacgcgc tgtgcatcag
      661 gcgccagctg cttcaggaca gcgacatcgg cgtaggcata cccctcggca agcaaggcat
      721 cgagaatcct cgccctggct tcctcaacct tgtcttcctg gtacgccttg ccctccttaa
      781 gcccgcccac ctccagcaag tcacctgtga ccacaggttc atcagacgcc accgaaatct
      841 ctcccaaacg gtactctggt ccctctgaga tttgaaacaa aacggatttc tgatctccaa
      901 agcggttctc cgtccaggtg ggctgggcca cttgcgcctc aaaaaatccc caccgggtgt
      961 attcattctg gaggtgatct cgatccttcc aaagtttatc ttggttaaaa tactgccgtg
     1021 accaaaagct cgattcctgg gacgccatca cctttttgag tttttttgtg cccagctcat
     1081 cattcccgtc aaaatgaata ttcgaaagtg cgacccgctt accggcttca atactgtatt
     1141 tggcaatgac ggattttttg tcgggcttga tgacgtcgga tgcgatctgg gcattgggaa
     1201 agccccgctg ttcataataa cgttgcagac gctctttccc gttattgatc gcgtagcggt
     1261 cataggagcg cagttcagcc agcttcgtga cctgctgcag acggcgctgt ggaaagaaag
     1321 aattcccctc aatctgtagt ttgaattttt tgttttcgcg aatcttcagg tacagatcga
     1381 cctttcgcgt gtcgttatga aacacgagtc cttccagact gaccctggcc ttgatgtacc
     1441 catgtcgtgc gtacattttt tttattttgg tcagattctt tttgagccgg ttgatgcgaa
     1501 agcgggaaat ctgcgacacc ttgcggcgga ttttctgttc tgaaaattga tgtgtccctg
     1561 tcacaaagat ctgatcgatg tgatacgtga ccccatgatg aatgcgtatg agcaggtcta
     1621 cgacatcatg tctctgttcg cgcttgacct caatgtccac acgcgattca aaaaaaccat
     1681 ttttctccag ataggttctg atggcctcaa cagactcagg gatcaaaccc ggttgaaagt
     1741 cggtccccgg ctgcagggca atcagctttt taatattttt cgccaggatg ggataattac
     1801 catcaacgtt cacattacgg actctcaagg ctttgtgtat gtcgaccttg agggcctgtc
     1861 cgccgatacc attcgacaac agctcataag tgatatcgga aaagagtccc gattggcgca
     1921 ttttctcaaa atgcacgcgc aagtcatctt cgttcaccac ctgacctttt ttgtacggca
     1981 ccactgtgtt caactgactt ttgaattcct ttggtccgtc aaaggcaatc acgacatccg
     2041 acaaaacaac ctcttcggga taaacatcac tcacaggcac aatgaaaaac aaggcgaata
     2101 cgagaaaaaa aatgagggat ttatgtacgt gtcctgacaa tttcattgtt tcaaaaaacc
     2161 gtgtttacgt gaatgtcgaa tattgaacaa ggaacaagga atttc
//
LOCUS       DATDUN010000848         1870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_454704, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000848 DATDUN010000000
VERSION     DATDUN010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454704"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VJC18_11805"
     CDS             <1..191
                     /locus_tag="VJC18_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46109.1"
                     /translation="ECKMSLRFKNNFLFGIQEYLLKRTREGTGFLFYGGVPLKEKQGM
                     CYVLPYYLVDSFSRWINK"
     gene            complement(203..1301)
                     /gene="prfB"
                     /locus_tag="VJC18_11810"
                     /pseudo
     CDS             complement(203..1301)
                     /gene="prfB"
                     /locus_tag="VJC18_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(1493..>1870)
                     /locus_tag="VJC18_11815"
     CDS             complement(1493..>1870)
                     /locus_tag="VJC18_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HLD46110.1"
                     /translation="RYARQFTRAGAQLLINYTNDAWYGNSSAQHQHLVFSQFRALENR
                     RFLARATNSGMTAIITPRGEVLDKLDPFKESFLFQNIKLDVANSYYTQHGDTWVFTIV
                     ILSGLLFVYTIFKRLSGPVKIEF"
BASE COUNT          416 a          450 c          474 g          530 t
ORIGIN      
        1 tggaatgcaa aatgtcccta agatttaaaa ataatttttt atttggaatt caggaatatc
       61 ttttgaaaag gactcgtgag gggacggggt ttttatttta tgggggggtg ccgcttaagg
      121 aaaaacaggg gatgtgctat gtcttgcctt actatcttgt cgacagtttc tcccggtgga
      181 taaataaatg accccttttt tactagtcct gcccctcact catcaaatat tccctgatca
      241 gatcatccaa atcgccatcc agcaccttgg cggtctggct ggtttcaaaa ttcgtgcggt
      301 gatccttgat catctggtac ggatgcaaga catatgagcg gatctgagaa ccccaggcga
      361 tgtccatctt gctgtcttcc acttttttct tctcagcgtt ctttttttcc agttccattt
      421 cgtacaatcg gcccttcaac atgttcatgg ctgtggcccg gttctggtgc tgggaacgct
      481 cggtctggca acggacaaca atccccgttg gtttgtgcgt gaggcgcaca gcggacgagg
      541 ttttgttgac cttctggccg ccggcaccac tggcgcggat ggcctcgaat tccaggtctt
      601 cttctttgat ctgaatgtcg atatcatcat tgacctgcgc gtagacaaac acggaacaaa
      661 atgaggtgtg acggcgattg ttggcatcga agggagaaca cctgaccaga cgatggacgc
      721 cgttctcggc cttgagatag ccgtaggcgc aagtcccgtc aatcttgagc gtgacgttgc
      781 ggtagcccgc gccgtctcct tcggtgaaat ccacaaccgt gctctggtat tttttggcct
      841 cagcccaccg caggtacatc cggcagagca tgtttgccca atcacaagat tccgtaccac
      901 ccgcaccggc attgatcagg aggtaggcgc tatagtgatc gagcggtccc gataacatct
      961 tggtgatctg tagtgcttca agatattttt ttgccctgat gagggacgcc tcgagctctc
     1021 ttttgttgtc ttcggaggga tcttctgtgt gaaattcgat caaaacctga atgtcgtcaa
     1081 cggacgattt gagttgatca aattcagcga tctgggcctt gagggtttcg atttccttca
     1141 tgacctcgcg tgcgtggtcg gcctgatccc aaaacccttc ctttagcgaa tcctgttcca
     1201 gtcctgcttt gcggtcgatt tttttgtcga cgtcaaagtg acctccgcaa tttcagggtt
     1261 tcgtcgagaa gagcttggtg ttcttgttgg tatgtggcca tgatgttctc cgaaaataca
     1321 aatgattgta atgagccgtt ggttgggata cgttttttcg ccaccactca aaaatttgcc
     1381 gcatgcaaat tttttcgccc gcaaaattca tggctgcaga tcatcacggg acatgaattt
     1441 cgcgaagtgt ctccaaaaca gatcccaacc aacggcgatc aactatcccc tttcaaaatt
     1501 caatcttcac cgggcctgag agtcttttga agatggtgta aacgaacaag aggccactca
     1561 ggatgacgat ggtgaatacc cacgtgtcgc cgtgttgtgt gtagtaggag tttgcgacat
     1621 cgagtttgat gttttgaaaa agaaaacttt ccttgaacgg atcgagcttg tccagaactt
     1681 ctccgcgtgg ggtaatgatc gctgtcatgc ccgaattggt ggcccgggcc agaaaacgcc
     1741 ggttttccaa cgcacgaaat tgtgaaaaga ccaggtgttg gtgttgtgcc gaagagtttc
     1801 cataccaggc atcgtttgtg taattgatca gcagttgtgc tcccgcgcgc gtgaattgtc
     1861 gcgcgtaacg
//
LOCUS       DATDUN010000849         1039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_122573, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000849 DATDUN010000000
VERSION     DATDUN010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122573"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1039
                     /locus_tag="VJC18_11820"
     CDS             <1..>1039
                     /locus_tag="VJC18_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLD46111.1"
                     /translation="ALPARHEWVKNQERITDENYQKYFENFNPDLYNPREWAKMAKAA
                     GMKYAVITTKHHEGFCLFDSKFTDYKATKTQYGKDLLKEWVDAFRAEGLKIGFYYSLI
                     DWHHPDFTVDRVHPQRTDDKAKLAELNKNRDMGKYREYLHNQVRELLTNYGKVDMLWL
                     DYSYPGENGKDNKDWDSEKLIKMVRQLQPSILVNDRLDLKDYEDGWDFTTPEQYKVAK
                     WPEVNGKKVAWETCQTFSGSWGYYRDEHTWKDTKQLLVLLIESVSKGGNLLLNVGPTA
                     RGTFDERAESALSSMGNWMKLNNRSVYGCTQAPDEFKAPENSLLTYNPKTNRLYIHLL
                     DYPLQNFNLPD"
BASE COUNT          317 a          230 c          246 g          246 t
ORIGIN      
        1 atgcattgcc tgcccggcac gaatgggtaa aaaatcagga gcggattacc gatgagaatt
       61 atcagaagta cttcgaaaat ttcaatcctg atttgtacaa tccacgcgaa tgggctaaaa
      121 tggccaaagc tgccggaatg aaatatgcgg tgattaccac caaacaccat gaaggtttct
      181 gtttgttcga ttcaaaattt accgattaca aagccaccaa aacacagtat ggcaaagatc
      241 tcctgaaaga atgggtggat gcttttcgtg ctgaaggctt gaaaatcgga ttctattaca
      301 gcctgatcga ctggcatcat ccagatttta cggttgaccg tgtgcatccg caacgaacag
      361 atgataaggc caaattggcc gaattgaaca aaaaccgcga catgggcaaa taccgtgaat
      421 acctgcacaa tcaggtgcgc gagttgttga ccaactatgg aaaagtggac atgctttggc
      481 tcgattattc gtatccgggc gaaaatggca aagacaacaa agactgggac tctgaaaaac
      541 tcatcaaaat ggttcgtcag ttacagcctt ccattttggt gaacgaccgt ctggacctga
      601 aagattacga agatggctgg gatttcacta ctcctgaaca gtacaaagtt gccaaatggc
      661 ccgaggtgaa cggcaaaaaa gtggcctggg aaacctgtca gactttcagc ggatcgtggg
      721 gttactaccg cgatgaacat acatggaaag acaccaaaca attgctcgtt ttgttgatcg
      781 aatcagttag caaaggcggt aatttactcc tgaatgttgg cccgacagcc cgcggaacat
      841 ttgacgagcg tgccgaatcg gcgctgagct caatgggcaa ctggatgaaa ttgaacaacc
      901 ggtcagttta tggctgcacg caggctcccg acgagtttaa ggctcctgaa aacagcctgc
      961 taacgtacaa tccaaagacc aaccgtttat acattcactt gttggattat ccgttacaga
     1021 atttcaattt gcctgatta
//
LOCUS       DATDUN010000850         1937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_165789, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000850 DATDUN010000000
VERSION     DATDUN010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165789"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VJC18_11825"
     CDS             <1..399
                     /locus_tag="VJC18_11825"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HLD46112.1"
                     /translation="RHGTPSASGYGTGGSHFGTKGEGRVSLASDEAIILGALDKSLID
                     AVIKRNLNQIRYCYQRELSKNPSLAGNISVKFVIAKDGTVSSATIRRSALNNTKVESC
                     LCDRFMRFQFPEPTGGGIVVAMYPFFFSPE"
     gene            608..1783
                     /locus_tag="VJC18_11830"
     CDS             608..1783
                     /locus_tag="VJC18_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLD46113.1"
                     /translation="MAPFFQKPIIKIISGMRRVGKSVLLHQIADKLVAQGLTQDRICF
                     IDMESLEFEHIKTHQDLAREVKKYFGSKKSNKCLFVDEVQEIQDWEKTIVSLAKNSEM
                     DIYLSGSNAHMLSSDLATRLSGRYIEIPVYPLGFDEFLEFRKAQQEDKKKEFLNYLQY
                     GGLPAIHYFPLQESTVYQYLRSIYDTILMKDIVRRFDIRNMPLLESIVRFVGDNLGNF
                     TSSNRIADYLKSQRIRMSVDTVLTYLGYLESARLVHGVSRFDVKGKRHLEFTKKYFWG
                     DIGLRHAVLGFREGELAGVLENIVYLELKRRGYEVSVGKLDQLEIDFIATKEKKKIYV
                     QVAYVLSSPKTVKREFEPLLAIDDNYPKYVVSTDEIFGNDYQGIQRLNIVDFLLGDA"
BASE COUNT          552 a          421 c          446 g          518 t
ORIGIN      
        1 agacacggca caccgtccgc cagtgggtac ggcactggcg gcagtcattt tggcacaaaa
       61 ggtgagggcc gcgtgagtct cgccagcgac gaggccatca tattgggggc actggataaa
      121 tccctgattg atgctgtcat caaaagaaac ctgaatcaga tccgatattg ttaccaaagg
      181 gaattgtcaa aaaatccgtc tctcgccgga aacatttcgg tcaaatttgt catcgcaaag
      241 gatggtacgg tatcttctgc aacgatcagg cgttcagccc tcaacaacac gaaagtcgaa
      301 agctgtctct gtgatcgttt tatgcgtttt caatttcctg agccaacagg tggcgggatc
      361 gttgtggcca tgtacccgtt cttcttctca ccagagtaag tacgtaccat tgcccatcac
      421 ccatcaccca catgccctga gcaaagtcga agggttaccc cttccctgaa tgacaccagg
      481 gcttaagggc acctaaatat agttgttgtc accaaaagcc tgttgagtta taatttcttc
      541 tatggaagaa actaatgcaa aaaactctaa aagtctcttg tataagagaa acttatatct
      601 cgaccgtatg gccccttttt ttcaaaaacc cattatcaaa atcatttctg gcatgcggcg
      661 ggttggcaaa agtgtgcttc tccatcaaat tgctgacaaa cttgtggcgc aaggactcac
      721 acaagacagg atctgtttta tagatatgga gtcattggag tttgaacaca ttaaaacgca
      781 tcaagatttg gcacgcgagg tgaaaaaata ttttgggtca aagaaaagta acaaatgtct
      841 ctttgttgac gaggtgcagg aaattcaaga ctgggaaaag accattgtct ctttggcaaa
      901 aaattcagaa atggatattt atctcagcgg atcaaatgcc cacatgctgt cgtcagatct
      961 ggccacacgg ctttctggcc gctatattga aatccccgta tatcccctgg gattcgatga
     1021 gtttctcgag ttcagaaagg ctcaacaaga ggacaagaaa aaagagtttc taaattatct
     1081 gcaatatggc gggttacccg cgattcatta tttcccactg caggaatcaa cggtttacca
     1141 gtatctccgc tccatctacg acacgattct catgaaagac atcgtcaggc gattcgacat
     1201 ccggaacatg ccactgctgg aaagcattgt gagatttgtg ggtgacaacc ttgggaattt
     1261 cacatcgtcc aatcgtattg ctgactatct caaatctcag cgcatcagga tgagtgtgga
     1321 taccgttctc acgtatctgg ggtatcttga gtcggccagg ttggttcacg gtgtttcacg
     1381 gtttgatgtt aagggaaagc gacacttgga attcactaaa aaatattttt ggggggatat
     1441 cggactacgc cacgctgtgt tgggatttcg ggagggggaa ttggcaggag tactcgaaaa
     1501 cattgtttat ctggaactca aacgccgtgg ctacgaggtg agtgtgggaa aattggatca
     1561 actggaaatt gattttattg ccacaaagga aaagaaaaaa atttatgttc aggtggccta
     1621 tgtcctttca tcaccaaaaa ccgtcaaacg tgaatttgag ccgttgctgg ccattgacga
     1681 caattatccc aagtatgttg tgagcacaga tgaaatattt ggaaacgact atcaaggaat
     1741 tcagcgactt aacatagtcg atttcttgtt gggtgatgca tgagggtgtg cgacaaattt
     1801 gtagttaaat gataatgata atcggcaccg cccctacttt tatgtcatac acacccttgc
     1861 caattgattg ttgaaataat tgccgtattt gcctagttag aaagccccaa aggagtcccc
     1921 atgtttccac acgccct
//
LOCUS       DATDUN010000851         1276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_331053, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000851 DATDUN010000000
VERSION     DATDUN010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331053"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1276)
                     /locus_tag="VJC18_11835"
     CDS             complement(<1..>1276)
                     /locus_tag="VJC18_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46114.1"
                     /translation="GSYSYIEVPDGASKTFHVQFNAWVFGTQTIYITITADIEGYIRL
                     PMRAIVTGPSSFKLIPTSYACTNDSAPQISSLDFYKVAYGQTRTEGIKLCNTGGEDIK
                     ITSATIENAGDALNSVAMDDNAFEDFLWQVSSEIDSTFALGESPSTTGSFVEPVFVDY
                     TGPVDDPAGSYSVRLDHNDGPVKDVLVEAGNILRIDVDYVPTLDVEAGEGQLYYAIAM
                     NAVLSLNTSLGAVQIPLLGATSGAEPMLEMSYRLSDTDEWKNIDLYSEGAALYFGTVD
                     IFLDWVSINSSTAQVKIKNTGSGSKDLEFYGGGINGFFEYFWEGDKKLSFPMSLSSGA
                     EAELSLRYLPSATGEIDDDYQARFDFGQFYFQHTGGNGPQGKATLVGEQDAGYAVELY
                     YGGTKLEREYSEGRNQNLCVFSTDTANPTPSTF"
BASE COUNT          318 a          365 c          288 g          305 t
ORIGIN      
        1 gaaggtagac ggcgtggggt tggctgtgtc cgtcgaaaac acacaaagat tctggttgcg
       61 accctcgctg tactcgcgtt cgagtttggt tcctccataa tacaattcca ccgcataacc
      121 cgcatcctgc tcacccacca gcgtggcttt gccctgcggc ccattgccac cggtgtgttg
      181 aaaataaaat tgtccaaaat caaaccgtgc ctgataatcg tcatcgatct ccccggtcgc
      241 cgaaggcaga tagcgcaggg acagttcggc ctccgcccca gaagacagac tcatcgggaa
      301 actcaatttt ttgtcaccct cccaaaaata ttcaaaaaaa ccatttatgc caccgccata
      361 aaattctaaa tctttcgacc cgctccccgt attcttgatc ttgacttgag ctgttgatga
      421 attgatcgag acccaatcca gaaaaatatc aaccgttcca aaatacaggg cagccccctc
      481 tgaatagaga tcaatgtttt tccattcatc ggtatcagac aggcgataag acatctccag
      541 catgggttcc gctccactcg tggctccgag caaaggaatc tgaacggcac ccagcgaagt
      601 attgaggctc aacacagcat tcatggcaat cgcgtagtag agttgcccct cacccgcctc
      661 aacatcaagt gtggggacat aatctacatc aatcctcaaa atgttccccg cttcgaccag
      721 gacatctttg accgggccat cgttgtgatc cagcctcacc gaataactcc ccgcgggatc
      781 gtccacaggc cctgtgtaat ccacaaacac aggctccaca aaagaccctg tcgtagaagg
      841 actctcacct agggcgaacg tgctgtcaat ttcgctggac acctgccaca ggaaatcctc
      901 aaacgcgtta tcatccatgg ccaccgaatt gagggcgtct cccgcatttt cgattgtggc
      961 tgaagtgatc ttgatgtcct caccacccgt gttacacagt ttgatcccct ctgttctggt
     1021 ctgtccgtaa gccaccttgt aaaaatccaa tgaggaaatc tgtggcgcag agtcattggt
     1081 gcaggcataa cttgtaggga tcagtttgaa cgaactggga cctgtgacga tggccctcat
     1141 cggcagtctg atgtagccct cgatatcggc tgtgatggtg atgtagatcg tctgtgtgcc
     1201 aaagacccag gcgttgaatt gtacatggaa tgttttggac gccccgtccg gcacttcgat
     1261 gtagctatag ctcccc
//
LOCUS       DATDUN010000852         1174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_122657, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000852 DATDUN010000000
VERSION     DATDUN010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122657"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..759)
                     /locus_tag="VJC18_11840"
     CDS             complement(181..759)
                     /locus_tag="VJC18_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46115.1"
                     /translation="MTLNFNVERYLQNQRGLSSQDSLGLWHEMAHAITDLIGERQGHS
                     LYTSGILATEFDKVQSVCQQGFHNGLPTVTPYAATNIMEFTAETMTAYFNDGREFPFL
                     VDDDLLTGGKFKNRLVQTSPLLCLAYTAFFSIDSPVRFDPWIFSDAALPYLERAEQEI
                     VHWPDRVKKDSDEDVQYAIEYLYRELTAHRTH"
     gene            complement(884..>1174)
                     /locus_tag="VJC18_11845"
     CDS             complement(884..>1174)
                     /locus_tag="VJC18_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46116.1"
                     /translation="WALFAQSKFSQGQNNRIEIVSKFLRMYGRWLRVVYQAGQIDVWT
                     HQNGEPVRTLFRQQPLMLRGRDLERVFEEIQQQVERFKRQAQAKNTASSEKT"
BASE COUNT          301 a          307 c          268 g          298 t
ORIGIN      
        1 tcagcgacat aagttcgaga cgcttttgag gcaaggagtg aaatgaggag ccagcggagc
       61 gtacacgcaa gtacgtgagc aggcaccgca atgaacgaca cagcatcaaa agatgtatcg
      121 gacttatgtc gctgagtata gcgtccagct agcacagcgc tcatataatg caacgtgatt
      181 ttagtgggtt ctgtgtgccg tcagctcacg atacaaatat tcaatggcat actgaacgtc
      241 ctcatcgctg tcctttttga cacggtcagg ccaatgcaca atttcttgtt cggccctttc
      301 caagtaaggc aatgccgcgt cagaaaaaat ccacgggtca aatctgacag gagaatcgat
      361 cgagaaaaaa gccgtgtagg ccaagcacaa caagggactg gtttgtacca gtctgttttt
      421 gaatttgccg cctgtgagta gatcatcatc aacaagaaaa ggaaactctc tgccatcgtt
      481 aaagtaagcc gtcatcgtct ctgccgtgaa ctccataata ttcgttgccg cgtacggggt
      541 gaccgttggc aaaccattgt gaaacccctg ttggcagaca ctctgcactt tgtcgaattc
      601 tgttgccaat atccccgagg tgtagaggct gtgcccctgt cgctcaccga tgaggtccgt
      661 gatagcatga gccatttcgt gccacagccc caagctgtct tgggaagaaa gccctctctg
      721 attctgaaga tatctctcaa cattgaagtt aagtgtcatc ttctgtgcat cataaaaagc
      781 aatggcgttg gataatgagc cgtcgcccca ctgccaatat ggctcagcaa aatcaaaact
      841 ccccctgccc cctcttgtta agagggggtt cttagaggcg gccttaagtt ttctctgatg
      901 atgctgtgtt tttggcctgc gcctgtctct taaaccgctc cacctgttgc tgaatctctt
      961 caaacaccct ttcaagatcc cgtccccgta acatgagcgg ctgttgtctg aaaagagttc
     1021 tcacaggctc cccattctga tgggtccaca catcaatctg ccccgcctga taaacaaccc
     1081 ttaaccagcg cccgtacatt ctgagaaatt ttgaaacgat ctctatgcga ttattctgtc
     1141 cttgagagaa cttgctttgc gcaaacaagg ccca
//
LOCUS       DATDUN010000853         4249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_580051, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000853 DATDUN010000000
VERSION     DATDUN010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580051"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..2420
                     /locus_tag="VJC18_11850"
     CDS             267..2420
                     /locus_tag="VJC18_11850"
                     /inference="COORDINATES: protein motif:HMM:NF028470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5123 domain-containing protein"
                     /protein_id="HLD46117.1"
                     /translation="MDDNETITVDQGNDSVSAPDTPTTGEENTNTVTDSEPDEASQIN
                     EYPSQKGTPPDYKKKKKPPLPSEKSDGSIPAKDGQPPVAGDDQYPIDDDAEETTDENP
                     VSGDANVPGCYEMASDACTSFSPYDSPECFIKKINAEIESNAAINTTYYVAITQGEYQ
                     AQGLQGCPFSGTVCPWGSDTNDGLTPEKPLATFKEAFSRMKDHKEANGKTGHYTLSIR
                     GGKFTYSEPIVVTAYAFYGAQMYKGPFLYDSNGNQVKGNTTTYEVSYYGDSDYADAKA
                     YIISDISIKPHQNESVTIDGGCAFTPPTGSDGQPVVFGHSEWDVDDTAMMRTCASPRW
                     KDPITDPYTAGFFGTMGMISIHNAHRITVEDINVINAPTFGIVIYGVSDHVVIKNNNI
                     SNTYASGVAAMFGPQNVIIDGNDVSYACAGSAQENISAKANVSHVAIINNRVTNSYND
                     AAIDVSTGYHHVYVGHNEVIGATTGGIYVEAQNVDSDHVWIDGNRIHDSVYGDCIRLA
                     SEQRANTEDVKITSNVCYNTLRGIWIAGYFHLIGENAKLDPTVVGVPMHNVSVLNNTV
                     YGTGNELMGFGSGFAMSNPGATNVVVENNIFSTSYKNTVSFQYVLRDDGTLNSDIIEF
                     KNNITFCSDSVEKDEEGNLKCLSDNAKEMPVAGNYQFDPLFADPENGDFHLSNDSTAI
                     DAGSHNTDAPDYDFECDLRLFDDNTTVDIGADEYY"
     gene            complement(2488..>4249)
                     /locus_tag="VJC18_11855"
     CDS             complement(2488..>4249)
                     /locus_tag="VJC18_11855"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HLD46118.1"
                     /translation="KLHDVDGLKDCEAVIDDYQSTCQKISHNMPKDLVKYFSTCYEVM
                     PVLSLRSSAKYQMKKFVEMGHELLCEHNLQELASKMLQFVQSLTKMENGFVKINDSKS
                     AEMVTVRSDPAHGSESSHSPRCEELADLVFAEGKPFIKTSAVQRDLQQFMTKHSELID
                     SIQKPSIIILPLLVNRTSMGVIYLDSPQKHIIKNEEDVELLISFVALMTNVIHNAPHL
                     EWRKFNSHESHTCSSVHHSGACDSKYHFDCFIGISEKVRNLIELMRHTVDTSTTVTLT
                     GNSGVGKEMIARILHYNSPRKSKPFITINCAAIPENLIESELFGHAKGAFTDAKENKM
                     GLFQKADQGTLFLDEIGDMPLNMQSKLLRVLQEREITPVGGTTNQKIDVKIICATNRN
                     LEKMVKSGAFREDLFYRISVIKIHVPSLCERKEDIPLLVDHALKMFAEENKTMQKNIS
                     HQAMSFLMKYAWPGNVRELVNVIYNLCIFVDKPIIELKDLEDRKELFSMPIDPSQGSE
                     NQSELSGLIQQIDAHQISLAEAKQAFERLQIERALKIFNGQITSASYHLQMPRPQVSR
                     LIKKYEIDKKKYKDNDERKE"
BASE COUNT         1160 a          971 c          971 g         1147 t
ORIGIN      
        1 aattgatctg gacaccacaa cgggtgtcgc tattgttgtg taaatacaat tatccacttg
       61 ttatcattgc gcattatttg cgctgaaatg gcgtacattt tgcatgtaac aagcacatga
      121 agagggatca cctgaagaga ccaatatgtc tcactgtttt gatcatgatt gtgatcatct
      181 gcgtacgatg tggcccagac catattactg gaataacaca agacactccg accagccaac
      241 agggacagcc agaagagtca gcgccaatgg atgacaatga aaccattacc gtggatcagg
      301 gcaacgattc tgtatcagcc cctgataccc ctacgacggg agaggaaaat acaaatacag
      361 ttacggattc tgaacctgat gaagcatccc agatcaatga atacccgtcg caaaagggaa
      421 cgccgcccga ctataaaaaa aagaaaaaac ctcccttgcc atctgaaaaa tctgatggat
      481 caattcctgc aaaagacggc caaccacccg ttgctggtga cgatcagtat ccgattgacg
      541 atgatgctga agaaacgaca gatgaaaatc ccgtttcagg cgatgcaaat gttcccggtt
      601 gctacgaaat ggccagtgat gcctgtacgt cattcagtcc ctacgattca ccggaatgtt
      661 ttattaaaaa aatcaatgcc gagattgaaa gcaacgccgc catcaataca acctactatg
      721 ttgccatcac acagggcgag tatcaagcac agggtctgca aggatgcccc ttttctggta
      781 ccgtgtgtcc atgggggagt gacaccaacg acggtttgac cccggaaaaa cctttggcca
      841 cattcaaaga ggcattttcc cgaatgaaag accataaaga ggccaatggc aaaacaggtc
      901 actacacgtt atccattcgt ggtggaaaat ttacgtattc tgaacctatc gtagtcacgg
      961 cttacgcgtt ttatggtgca cagatgtata aaggaccatt tttgtacgac agtaatggta
     1021 atcaggttaa gggcaacacc acgacatatg aggtcagtta ttatggtgac agtgactacg
     1081 ctgatgccaa ggcttacatc atttcggaca tttccatcaa accccaccaa aatgaatcag
     1141 tcaccatcga tggggggtgc gccttcacac cacctactgg ttcagatggt caaccagtgg
     1201 tgtttggaca cagtgaatgg gatgtcgacg acaccgccat gatgagaacc tgtgcctccc
     1261 ctcgctggaa agaccccatc acagacccct acacggctgg tttttttggc accatgggca
     1321 tgatttcgat tcacaacgcg caccggatca cggttgagga catcaacgtc atcaacgccc
     1381 ccaccttcgg tattgtgatc tatggggtct ctgatcatgt ggtgatcaaa aacaataaca
     1441 tcagcaacac ctacgcgtca ggtgtggcgg ccatgtttgg ccctcaaaat gtgatcattg
     1501 acggcaatga tgtgtcctat gcctgcgcgg gaagtgcgca ggaaaatatt tctgccaagg
     1561 ccaacgtcag ccatgtggcc atcatcaata acagggtgac caattcgtac aatgatgcgg
     1621 ccattgacgt gagtacaggc tatcatcatg tgtatgtcgg tcacaacgaa gtgattggtg
     1681 ccacgacggg cgggatctat gttgaagcgc aaaatgtgga ttccgatcat gtctggatcg
     1741 atggcaatcg cattcacgat tctgtgtatg gtgactgtat ccgtttggcc agcgaacagc
     1801 gggccaatac agaagatgtc aaaatcacca gcaatgtctg ttacaacaca ttgcgaggca
     1861 tctggattgc cggttatttt cacctgatag gggagaatgc caaattggac cccacagttg
     1921 tgggggtgcc catgcacaac gtgtctgtac tgaacaatac ggtatatggt acgggtaatg
     1981 aactcatggg atttggttct ggctttgcca tgtccaaccc gggagccacc aatgtggtcg
     2041 tggagaataa catcttcagt accagttata aaaacacggt ttcattccag tatgtgttgc
     2101 gtgacgatgg cacactcaat tccgacatca tcgaattcaa aaacaacatt accttctgtt
     2161 cggacagcgt agaaaaagac gaagagggaa atctgaaatg tctgtctgat aatgccaagg
     2221 aaatgcccgt ggcaggtaat tatcagtttg atccattatt cgcggatccc gaaaacggtg
     2281 attttcattt gagcaacgat tccactgcta ttgatgcggg aagccataac acagacgcac
     2341 ccgattacga ttttgagtgt gatttgagac tgtttgacga caataccact gttgatatcg
     2401 gggcggatga atactattga atacagaata ttgaatattg aacaaggaat gtcgaagtta
     2461 tattctgctt ttgaccttcg ctattcgtta ttctttcctt tcatcattat ccttgtattt
     2521 cttcttatca atttcgtatt ttttgatcag acgggaaacc tgaggtcgcg gcatctgcag
     2581 gtgatagctg gccgatgtga tctgtccatt gaaaatttta agggcccgct caatttgtaa
     2641 acgttcaaag gcctgcttgg cctctgccag ggaaatctgg tgggcatcga tctgctgaat
     2701 gagaccagat aattccgatt gattttctga cccctgtgat gggtcaatcg gcatactaaa
     2761 caattccttc cggtcctcca aatccttgag ctctatgatg ggtttgtcta caaagatgca
     2821 aaggttgtag ataacgttaa ccagttcacg cacattgcct ggccaggcgt atttcattaa
     2881 aaacgacatg gcctgatgcg aaatgttttt ctgcattgtt ttgttttctt ccgcaaacat
     2941 ttttagcgca tgatcgacta acaaagggat gtcctccttg cgttcacata aggaggggac
     3001 atgaatcttg atgactgaaa ttctgtaaaa taaatcttcg cggaaagcgc cactcttgac
     3061 cattttttcg aggttgcgat tggtcgcaca tatgattttc acatcgattt tttggttcgt
     3121 tgttcctccc accggtgtga tttccctttc ctgcagaacc cgcaacaact tggactgcat
     3181 attcagtggc atatcgccaa tttcatcgag aaacaaggtg ccctggtctg ctttttgaaa
     3241 caagcccatt ttgttttctt tggcatcggt aaaggctccc ttggcatggc caaagagttc
     3301 actttcaatc aaattttccg ggatcgccgc gcaattgatg gtgatgaagg gtttagattt
     3361 tcgcgggctg ttgtaatgga gaatccgggc aatcatttct ttgcccacac cggaattccc
     3421 agtcaacgtg actgttgtgc tcgtatcgac agtgtgccgc atcaattcaa tcagatttct
     3481 cactttttca gaaattccaa taaaacagtc aaaatgatat tttgaatcac acgcaccact
     3541 atggtgtaca gaagaacacg tgtgtgattc atgagagttg aatttgcgcc attccagatg
     3601 tggcgcgtta tgaatgacat tggtcatcag ggcaacgaag ctgatcagga gttccacgtc
     3661 ctcttcattt ttgatgatgt gtttttgggg actatccaga taaatgactc ccatactggt
     3721 tcggttgacc aaaagcggca ggatgatgat cgacggtttc tgaatactgt caatcaactc
     3781 gctgtgcttg gtcataaact gttgcaaatc cctttgcaca gcagaggtct tgatgaaggg
     3841 tttgccttct gcaaaaacca ggtcagccaa ttcttcgcaa cggggagaat gagaagactc
     3901 agacccatga gcgggatcag agcgaactgt caccatttcg gcagacttgc tgtcgttaat
     3961 tttaacgaaa ccattttcca tttttgtcaa agactggaca aactgcaaca tcttggacgc
     4021 caattcctgc aaattgtgct cacacagcag ttcgtgaccc atttcgacaa attttttcat
     4081 ctgatatttc gctgatgatc tcagcgacaa tacgggcatg acttcatagc acgttgaaaa
     4141 atatttaacg agatccttgg gcatgttgtg gctaatcttc tggcacgtgc tttgatagtc
     4201 atcgataacg gcttcgcaat cctttaagcc atccacatcg tgaagtttt
//
LOCUS       DATDUN010000854         1514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_248050, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000854 DATDUN010000000
VERSION     DATDUN010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248050"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..>1514
                     /locus_tag="VJC18_11860"
     CDS             505..>1514
                     /locus_tag="VJC18_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLD46119.1"
                     /translation="MSVKDLQSSDGGVGVETTNGCGLQGPGRPGHPSITDAQIVDMMV
                     AELFNPDFCEKNRRSTSIFLEPYDAIHEPFLHEPYPLNCIGPVARLTAENLEANVNIG
                     RLLVLLGNHTSNATLRALVEEAGRDDVVFEKLRLCANRAMHRIGSLAYAFEWRDNVTF
                     ENIDTLLEGLAAYRESNLRIARQMTARLEQLLSQRRCVTVLDLGSGVGGTSSELLNHL
                     ATLAQQNRIPANYKSLLHFVLYDISLPQLTVAKTHLQKAYGITDIDCRNGALDAVADV
                     LADVQNTVDVVVSGAAICHVLDKETFFSHLYRIMTPGSVMSFWDPAFGIYQADVIKVC
                     REG"
BASE COUNT          398 a          297 c          395 g          424 t
ORIGIN      
        1 aatgcagttc gcgaatcgga cagttcgata gtctctgcgg atcaggtggc acaaattaca
       61 gaagaaatga accagtatct cagggaatat ggagaaaaaa gtttgtttaa ttatccacat
      121 tatttggtag ttggacaaaa atgatgattg ggattgtgtc ggttacgaaa ataaaaaaag
      181 tagcaacaag taggaggggt cacacgatac aactcatgaa aatgcagaat tgatcagaac
      241 ttagcagtcg gtgtatgcga tctagggggc aatgtcactg attaacagcg gagtgtattt
      301 atgaggacat cgatgttgtc tgcaaaccca atagcactaa cattgccatt atgtgagagg
      361 cgcattgttg ggcgtggcct gtttcataag gactttggaa taagcagttg cggcaataca
      421 acgtcacctt tgttaacaca ttgttgtggt gacagggggc aggtggttct accaaatgcc
      481 agacgacacg cggcgcgtat tcatatgtca gtaaaagatt tgcagagttc ggatggcggt
      541 gtcggtgtgg aaacaacgaa tgggtgtggt ttgcaaggcc cgggtcgtcc cggccacccc
      601 tccatcaccg atgcacagat cgttgacatg atggtggccg aactgttcaa tcctgatttt
      661 tgcgaaaaga atcgtcgttc gacctcgatt tttttggaac cttatgatgc catccatgag
      721 ccttttttgc acgagcctta tccgttaaat tgcattggtc ccgtagccag actgacagca
      781 gaaaatcttg aggccaatgt taacattggt cgtttgttgg tattattggg aaaccacacc
      841 tcgaatgcaa cattacgagc ccttgtagaa gaagcgggca gggatgatgt tgtgtttgaa
      901 aaattaaggc tctgtgctaa tcgagccatg caccgcattg gaagtctggc ttatgccttt
      961 gaatggcgtg ataatgtgac ttttgaaaat atcgatacac tgctcgaggg tttggccgct
     1021 tatcgcgaaa gcaatctgcg cattgcccgt caaatgaccg ccagacttga acaactattg
     1081 tcccagagaa ggtgtgtgac tgttttggat ttgggatcgg gtgttggggg cacgagttcg
     1141 gagttgttga atcacctggc gacattggcc caacaaaaca gaatacccgc aaattacaag
     1201 agccttttgc attttgtgct gtacgacata tcgctaccgc aattgactgt cgccaaaact
     1261 catttgcaaa aggcatacgg catcacagat attgattgca gaaatggtgc tcttgatgcc
     1321 gtggctgatg ttcttgctga tgtacaaaac actgttgatg tggtggtttc tggtgcggct
     1381 atttgccatg tgctggacaa agaaaccttc ttttctcatt tgtatcgcat tatgacaccc
     1441 ggttccgtca tgagtttttg ggatcctgct tttggtattt atcaggcaga tgttatcaag
     1501 gtttgtcgtg aggg
//
LOCUS       DATDUN010000855         1145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_455189, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000855 DATDUN010000000
VERSION     DATDUN010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455189"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VJC18_11865"
     CDS             complement(<1..741)
                     /locus_tag="VJC18_11865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HLD46120.1"
                     /translation="MQKTETSPTQPRDMKLFWQTLLMTVFIVSFTIVCTEKAQAGNPL
                     YMAADNTPLIWDTTQTIKYKVDPKGLGKLSYSESVALVQIALSVWENVADVNIKFEYL
                     GPLNEALNADNWESVASNNIYAEAQDTTNMTATQSQQQHYVVIGFDNTGEIMTAKGSA
                     NATGVQSITGVLGTYEDPQFITSAHVFINGLYYNGTDNDAVDLNLVDLLAIVIHELGH
                     TLGLDHTLFNYDIYQDILSGSIDSSNARF"
BASE COUNT          261 a          262 c          300 g          322 t
ORIGIN      
        1 aaaacgtgcg ttgctggagt caatggagcc agagaggatg tcctggtaga tgtcataatt
       61 aaacagggtg tggtcgagtc ccagggtgtg cccgagttca tgaatcacga tcgccaacag
      121 gtccacgaga ttcagatcga cggcatcatt atctgtgcca ttgtaataga gtccattaat
      181 aaagacgtgc gcgctcgtga tgaattgggg atcctcatat gtccccagca ctccggtaat
      241 ggactggaca cctgtggcat tggcggagcc cttggctgtc atgatttcac cggtgttatc
      301 aaacccaatg acgacataat gttgttgttg tgactgggtc gccgtcatat tcgtggtgtc
      361 ttgggcttcg gcatagatgt tgttggaagc gacactctcc cagttgtctg cattcagggc
      421 ctcattgagg ggcccaaggt attcaaactt gatattcacg tcagcaacat tttcccaaac
      481 ggacagggct atttgaacca gggccactga ttctgaataa ctcaacttgc ccagtccttt
      541 ggggtcgact ttgtatttga tggtttgagt tgtgtcccaa atcagtggtg tgttgtcggc
      601 ggccatatac aggggatttc cggcttgggc tttttcggtg cagacgatgg tgaacgaaac
      661 gatgaagacg gtcatgagta gtgtttgcca gaagagtttc atatcacgtg gctgagtagg
      721 tgatgtttcg gttttctgca tgcgttggct cttggagaat cattcgaaaa cccgttgaga
      781 gtcacttttt tgggtcctgc cacggttcgc tattttttaa tggcgtcaca aagctacgct
      841 ttttgatttt atgtgactac cgcgttcact tgtgctcgct taggctcgca cacacttagt
      901 cactctgggc tcgcaagctc gcccagcggc gtgctcgcct gttcgcgcaa aaacagctca
      961 ccgtgtcacc caaaaaagat ctcttcgcgg taacttttcg aataggcccc tattatcggg
     1021 tcaaaatggc aaacgttgcc ccttttttga ataaaaaata ggattgatat atcaagtggt
     1081 taatagccag cggccagact atcgggacaa cggctatcgg cggccccata aaagctgtcc
     1141 ttgtt
//
LOCUS       DATDUN010000856         3690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_455300, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000856 DATDUN010000000
VERSION     DATDUN010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455300"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VJC18_11870"
     CDS             complement(<1..594)
                     /locus_tag="VJC18_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46121.1"
                     /translation="MNLQKNHHVRIIFLVIAALSFQFSHEAVAQNHSDGDHSLVMLNE
                     SRLPQNALGNLGVMTRHVNRILSDNGINTEQLIAVEEQLGTFEVSLPLSAQKTQLEQD
                     PRIDIVAVNHLFHVADEEPGAELLPAETTSWGITSVGGPGTYTSGTAWILDTGVSTTT
                     GDLNVDTTNAAYFIGDSFEDDNRHGTHKAGIIAAIDND"
     gene            814..1788
                     /locus_tag="VJC18_11875"
     CDS             814..1788
                     /locus_tag="VJC18_11875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HLD46122.1"
                     /translation="MKIISGDRTDQFTADKSIKFFESDTCDSGDATYAAEDAQGISHD
                     DILDSAQNETGADQVAAFVTILAGDSNQSETSKVYSGTGVTTPNGKTYRDGIVSIPGG
                     QFEMGSVGDNSDEHKSDGTTVTVTMSGFKLSKSEVTVGQYKAYLAATGDTQYSSLPDF
                     KENQKGDRFPVVGLTYDEKIAFCEYYGGTLPTAAQIEYATKGPSHTDVCGTPFDKAAF
                     WKNGFRTTAEVCGSNNERVNGWDVCDLTANVSETTRDQYDPVFYNRMPPVNPSNPLTD
                     SNQQQVEIRNGAWFDGEWSFRPAYRNSVDANYRYFFLGFRCAWSQVSQ"
     gene            complement(2050..3282)
                     /locus_tag="VJC18_11880"
     CDS             complement(2050..3282)
                     /locus_tag="VJC18_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46123.1"
                     /translation="MIVQGSFTDHFVANPVLQPKLAVANYVASQGFLVPTRYDSLDEA
                     LESQKPFIMRSEHPQDYAGASGLMQSYFVKPDVVSSLKMPINHFETNQLSRTDMISFL
                     NLLGPKIRGESVGPFDEAVARLSSENMTDYCHYMGLSEELFIKDISYSYWMEQTGFNI
                     SIVADNTIPGRYHITLRGKGVSYILREGNDVLKMATTENDTAVVQAISTRMVPWYEAI
                     RSLPHFNPQHCPIIEAQFFNGAIYFLQYLRARDFNPAQFVLDRSLEEGEQEALWVRGA
                     TSPDGHIFQTAIDYPEIIHHGEYFFPVLDAEAGSANSITSHMYNEIMSRRREVQFCNF
                     GDDKSVLPHIADGHLKMTLLFKPRISLIANVWQNVSPELNEKREAMLEKNMVPRIPVR
                     VVADGRKAYFKVIDWNPT"
BASE COUNT         1020 a          867 c          825 g          978 t
ORIGIN      
        1 atcgttatca atggcggcga taataccggc cttgtgagtg ccatgtctgt tatcgtcctc
       61 aaaggaatct ccaataaagt atgccgcgtt cgttgtgtca acgttcaagt cccccgtagt
      121 ggtagaaacc cccgtgtcca agatccaggc tgttccgctg gtgtaggtcc cggggccccc
      181 aacgctcgtt atgccccagg acgtggtttc tgccggcaac aattctgcac cgggttcttc
      241 gtcagcaaca tgaaaaagat gattgacagc cacgatgtcg attctcggat cttgttcgag
      301 ctgtgtcttt tgtgccgaca ggggcaacga gacttcaaac gttcccaatt gttcttcgac
      361 cgcgatcagc tgttcggtgt tgatgccgtt gtcactgaga atcctgttca cgtggcgggt
      421 catcacaccc aggttgccca gcgcattttg cgggagtcgg ctctcgttca gcataacaag
      481 agagtgatcc ccatcgctgt ggttttgtgc cacggcctcg tgagaaaact gaaaactcag
      541 ggccgcaatg accagaaaaa tgatcctcac atggtgattc ttttgcaaat tcatattgtc
      601 ctccggatta gggttgtggg ttattcagca tcggcaaggg tttgtgatta gtttcctaaa
      661 aaaaccatgt ttcgtactct ggagagtaca ctttttgaat ccacccgtac tctttagagc
      721 acgaaatcgt gtaaattgag tgaacaatac tacagcacag ccgatagacc ggacgatggg
      781 ggataattga agatttattt tttggagcga actatgaaaa ttatatctgg cgatcgaacc
      841 gatcaattca cagctgataa atcgataaaa ttttttgaat cagacacctg cgattctggt
      901 gatgccacct acgcagcaga agatgctcag ggtattagcc acgatgacat tttggattca
      961 gcgcaaaatg aaacaggtgc agatcaagtt gcagcgtttg tgacaatctt ggcaggtgat
     1021 tcaaatcaat cagaaacctc caaagtttac agtggcaccg gcgttaccac tcctaatggc
     1081 aaaacataca gagacggaat tgtttcaatt cccggtggcc agtttgagat gggttcggtc
     1141 ggcgataatt ccgatgaaca taaatctgat gggacaaccg tgactgtgac catgtcgggt
     1201 tttaaacttt caaaatccga ggtgactgtt ggccaataca aggcctatct tgcggcgact
     1261 ggagacaccc aatattcaag ccttcctgac tttaaagaga atcaaaaagg cgatcgtttt
     1321 cctgttgtgg gtttgaccta tgatgaaaaa atagctttct gcgaatatta cggaggaaca
     1381 ctacccacag cggctcagat tgaatatgcg accaaaggac caagtcacac cgatgtttgt
     1441 ggtacccctt ttgataaagc agccttctgg aaaaatggtt ttagaacaac cgctgaagtc
     1501 tgcggaagca acaatgaacg tgttaatggc tgggacgttt gtgatctgac agcaaatgtt
     1561 tcggaaacca cccgcgatca atatgaccct gttttttata atcggatgcc accggtcaat
     1621 ccaagcaatc cccttacaga ctcaaatcaa cagcaagtcg aaattcgcaa cggcgcatgg
     1681 ttcgacggcg aatggagttt tcggccagca tatcgcaact ccgtcgatgc caactaccgt
     1741 tacttctttc tcggtttccg ctgcgcttgg tcccaggtct ctcagtaagt caaggtctct
     1801 cagccagact tttttctgtg aaggcaaaca aacgcgtgtt tggcgggagt ccaatcgttg
     1861 ttcgtctgta tttcgagaat taagagcacc ccctataaac ccttttcgtt acgaaaaatc
     1921 acattcagag tcgaggacga taagaataaa acccgcctgc gcggccgtag ccgtttcggc
     1981 gaggcgaagg tgtgaatctt gtttttaaca atcccagatt tcgtactctg gggagcaagg
     2041 ggaaaatgat caggtgggat tccaatcgat aaccttaaaa taggctttgc gaccatcagc
     2101 aacaactcgt accggaattc gtggcaccat atttttctct aacatagctt ctcgtttttc
     2161 attcagttca ggagaaacat tttgccaaac atttgctatc agactgatcc tcggtttgaa
     2221 caacaatgtc atcttgagat gaccgtcagc aatatgtggc aaaacagatt tatcgtcacc
     2281 aaagttacaa aactgtactt cgcggcgacg cgacatgatc tcattgtaca tatgggaagt
     2341 tattgaattg gcagaaccag cctccgcatc caaaacagga aaaaaatact ctccatgatg
     2401 aatgatctcg ggataatcga tggctgtttg aaatatgtgt ccatcgggtg atgtggcgcc
     2461 ccggacccac aacgcctctt gctccccttc ctctaaagat ctatcgagaa cgaattgtgc
     2521 cggattaaaa tcacgagcac gaagatattg taaaaaatag atggccccat tgaaaaactg
     2581 ggcctctatg attggacagt gttgaggatt aaaatgcggc aaactgcgta tcgcctcata
     2641 ccagggaacc atgcgcgtcg agattgcttg cacgacggcc gtatcgttct cagttgttgc
     2701 catcttaaga acatcatttc cctctctaag aatataggag acccccttcc cccgcaatgt
     2761 aatgtgatat ctccctggga tagtgttgtc agccacaatg ctgatattga atcccgtttg
     2821 ctccatccaa tatgaataac tgatatcttt tataaacaat tcttccgaca gccccatgta
     2881 gtgacagtaa tccgtcatat tttcagatga tagccttgcc accgcctcat caaaaggacc
     2941 cacagactcc cctctgattt ttggccccaa aagatttaaa aaagaaatca tgtcagttcg
     3001 agacaattga tttgtttcaa aatgatttat tggcatttta agcgatgaca caacatcagg
     3061 ttttacaaag tagctctgca tgagaccgga ggcccccgcg tagtcctgcg ggtgctcact
     3121 gcgcataata aatggttttt gactctcgag ggcttcatct aacgaatcat atctggttgg
     3181 gaccaaaaaa ccctgagacg caacatagtt ggcaacagcg agtttgggtt gtaaaacggg
     3241 attagcaaca aaatgatctg tgaacgagcc ttgaacgatc atgaacgctt ccctttgaaa
     3301 aatgtgaaag gcaagtggcg catggaacca attaccccat catgacttca tttatcctaa
     3361 aaacagcagg tgaactgccg tttctaggtt tatgacaacc caaaatccct tttcatcctt
     3421 attatcggtt ttgttctgaa ccccgtgcac tgtaaaatgc agatttcgta ctctcaagag
     3481 tacacaaatc actctttatt gtgaacaaag gtggggcgtt gacgataata gaggtatcaa
     3541 actgtatttc tggccaagcc aggttaggcg tgaacaatga ataaattaaa aaaaatgatg
     3601 tcccttctat cttgctttct tctcatgaca acgatcgccc acgccgcggc cacagaggtc
     3661 gctgtcgata ccgccactaa tacggtaacc
//
LOCUS       DATDUN010000857         1335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_538704, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000857 DATDUN010000000
VERSION     DATDUN010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538704"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VJC18_11885"
     CDS             <1..893
                     /locus_tag="VJC18_11885"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLD46124.1"
                     /translation="QSLRARCKSAYDDGFLMDVALQIDGAKGTDLDTFSNQISGWETQ
                     WQSYFNQRMGELEAGRISMQNLAAVFRGACHAERWSKAGLDPIKFFDNLNEDEKKTVY
                     EAILLRYYFKNMIRLRWDEDQSDTTVFMKTKKGNCTVLNVMAQHMLRTTGSNLYRSLI
                     LPNHITLTLQTSQGQLYFETSGDLLFYPPGYYEKLTDHEFIRRNDQTEIEPWQTLGAV
                     YSNAGYRAVLDRDWEKARVALEAALDIVPDSTNANHFMGKTLMQLGDREGAIRHFRRA
                     LDSSPGYTDSQRLLDELNAG"
     gene            complement(890..>1335)
                     /locus_tag="VJC18_11890"
     CDS             complement(890..>1335)
                     /locus_tag="VJC18_11890"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HLD46125.1"
                     /translation="ITDASAQLGALLIDGIGDSLQIDSEQSLSENLNFCYSLLQACRV
                     RISKTEYISCPSCGRTQFDLQAVLAKIKARTAHLKGLKLGVMGCIVNGPGEMADADFG
                     YVGMGTGKVCLYVGQTCVERNIPEDCALDKLISLIKREGKWVEDY"
BASE COUNT          359 a          306 c          345 g          325 t
ORIGIN      
        1 cccagtcgtt acgcgctcgg tgcaagtctg cctatgatga cggattcctt atggacgtcg
       61 cattgcaaat tgatggcgcc aaagggactg atcttgatac tttttccaat caaatatcag
      121 gatgggaaac tcagtggcaa agttatttta atcagagaat gggagagttg gaagctggcc
      181 gcatttcaat gcagaatttg gccgctgtgt tccgtggagc ttgtcatgca gagcgatggt
      241 caaaagcggg attggatcca ataaaatttt ttgataatct aaatgaagat gaaaaaaaaa
      301 ccgtttatga agcgatttta cttcgttatt attttaaaaa tatgattcgt ctgaggtggg
      361 atgaagatca atcagataca acggtattca tgaagacaaa aaaggggaac tgtactgttc
      421 tcaatgtcat ggctcagcat atgctgagaa caacaggctc aaatctgtac aggtcactta
      481 tcttgccaaa tcacatcaca ctgactttac agacatctca gggacaactg tatttcgaaa
      541 catcgggtga tttgctgttt tatcctcctg ggtattatga aaaattgaca gatcatgaat
      601 tcataagacg aaatgatcag acagaaattg aaccttggca gaccttgggg gcagtgtata
      661 gcaatgccgg gtatcgtgcg gtgctggaca gggattggga gaaggcgagg gtagcgttgg
      721 aagcggcact ggatattgta cccgatagca ccaatgccaa tcactttatg ggcaagaccc
      781 tcatgcagct gggcgaccgt gagggcgcca tcagacactt ccgaagggcc ctggattcaa
      841 gtcccggcta cacggattcc caaaggttgc tggacgaatt gaacgctggt tagtaatcct
      901 ccacccactt cccctcacgt ttgatgagag agatgagttt gtcgagggcg cagtcctcgg
      961 gaatgttgcg ttccacacag gtttgcccca cgtacagaca caccttgccg gtccccatgc
     1021 ccacataacc aaaatcggcg tcggccattt ctcccggtcc attcacaatg caccccatca
     1081 caccgagctt gaggccttta aggtgcgctg tgcgcgcctt gatcttggcg agcacggcct
     1141 gcagatcaaa ctgggtgcgc ccacaggacg gacacgagat gtattctgtt ttggaaatgc
     1201 gcacccgaca cgcctgcaat aaggaataac aaaaattaag attttcagag aggctctgct
     1261 cgctgtcaat ctgcagggag tcgccgatgc catcgatgag gagcgctccc agttgcgcgg
     1321 aggcgtcagt gatgg
//
LOCUS       DATDUN010000858         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_248262, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000858 DATDUN010000000
VERSION     DATDUN010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248262"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VJC18_11895"
     CDS             complement(<1..957)
                     /locus_tag="VJC18_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLD46126.1"
                     /translation="MKIPVLIVANLVLFYFVYQFLRKRNSLSFYEGGKWWLTWVAIAV
                     ITLMDELTSIFYAPAEAFRHIGISAIFFIALTSVIMRVLSNRMVEIAHVLETNKLKGG
                     GVYNFAYLVMGPSLSFIAVASIYVAYVLTAAMSTVSAVENGMTFFNMSTPTKFALEFV
                     AVWLIAGLNIIGIKDNAKVTFGIFVFTAFVFVNFIVSGFFNMSAVHYDVVIDSSREAV
                     RASGRGFFTSAALIVASVSNCILAYSGIESVLQTARLVKDWQQIRKAYLFLALTVGIV
                     TPVITALVLSHTEFDPLQHVTDLIPRYAAMLNGEWFGMLMSLV"
     gene            1132..2052
                     /locus_tag="VJC18_11900"
     CDS             1132..2052
                     /locus_tag="VJC18_11900"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HLD46127.1"
                     /translation="MVQKVEKIWMDGKFVNWDDANVHILTHTLHYGLGIFEGVRCYKL
                     HDGRSGFFRLRDHLRRFNDSAKMYWLKPIYSIDELVKNSIELYHQNKMKEGYLRPLMY
                     IGSGAMGLHAIDNPLHTSLIAWEWGKYLGDDGFKNGIRAKISSISRLRPNINFVKAKA
                     GGNYPNSILAKREAMQDGYHEAIMLDTDGYVAEASGENIFVIRDGIVRTPPATASILN
                     GITRDSVIQLLRDDNIPLREEKILREELYVADEIFFTGTAAEVTPVREIDHHMIGDGK
                     PGPITTHVKELFFDAVRGKIAKHHEWLEIF"
BASE COUNT          662 a          592 c          520 g          514 t
ORIGIN      
        1 caccaaactc atgagcatcc cgaaccattc gccgttcaac atggcggcat acctggggat
       61 caggtctgtg acatgttgca aaggatcgaa ctcggtgtgg gataatacca aggccgtgat
      121 cacgggagtc acaatcccca cggtcagggc caaaaagagg taggcctttc tgatctgttg
      181 ccaatccttg accagacgcg ccgtttgcaa aacagattca ataccggaat aggccagaat
      241 acaattggat acgctggcca cgataagcgc cgcggaagta aaaaaacctc gtccagaggc
      301 acgcacggcc tctctggaac tgtcaatgac aacgtcatag tgaactgcgg acatgttgaa
      361 aaaaccagac acaatgaaat tgacgaacac gaaggccgta aagacaaaaa taccaaaggt
      421 caccttggcg ttgtccttga ttccaatgat gttcaaaccc gcaatgagcc acacagctac
      481 aaattccaag gcaaatttag taggcgtcga catgttgaaa aaagtcatcc cattttcaac
      541 tgcgctcact gtcgacatgg ccgccgtcaa cacgtaggcg acataaatag aggccacagc
      601 aatgaacgac aatgatggcc ccataaccag atacgcaaaa ttgtacaccc ccccaccctt
      661 gagtttgttg gtttccagca cgtgggcaat ttccaccatg cggtttgata atacccgcat
      721 gattaccgaa gtcagggcaa taaaaaaaat ggcagagatg ccaatgtgcc ggaacgcctc
      781 ggccggggcg taaaagatac tcgtcagttc gtccatgagc gtgatcacag cgatcgcgac
      841 ccaggtcagc caccacttgc cgccctcgta aaagctcaat gagtttcttt tgcgcaaaaa
      901 ctgatagaca aaataaaaca ggaccagatt ggcaacgatg aggaccggta ttttcataaa
      961 aggaggggtt cgctatcaat tttaaccaaa agatgcaatc gcgataaatc acatcttgaa
     1021 atgcgtcatt gaaccatggc gcattacctg ctccacaata tttcgcttga tttgttctta
     1081 ataattagta atcccgccat gatagcagtt ctaccaacaa ggggttcata tatggttcaa
     1141 aaagttgaaa aaatttggat ggatggcaaa tttgtaaact gggacgacgc caatgtgcat
     1201 atcctcactc acacattgca ttatggactg ggcatcttcg agggcgtgcg ttgctacaaa
     1261 ttgcatgacg gccgatcggg cttttttcgc ctgagggatc atctcagacg cttcaacgat
     1321 tccgccaaaa tgtattggct caaaccgatt tattccatcg acgaactggt caaaaactcc
     1381 attgagcttt atcatcaaaa taaaatgaaa gagggttacc tgcgaccgct catgtacatc
     1441 ggttccggcg ccatgggcct gcacgccatc gataatcccc tgcacacatc actgatcgcc
     1501 tgggaatggg gcaagtatct gggtgacgac ggctttaaaa acgggattcg cgcgaaaatc
     1561 tcgtcgatca gccgcctgcg tcccaacatc aattttgtca aggccaaggc agggggcaat
     1621 taccccaatt ccattttggc caaacgcgag gccatgcagg acggctatca tgaggccatc
     1681 atgctcgata ccgatggcta tgtcgccgag gcatcgggcg agaatatttt cgtcatccgc
     1741 gatggaattg tgagaacccc acccgccact gcgagcatac tcaacggcat cacccgggat
     1801 tctgtgatcc aactgttgcg tgacgataac atccccctgc gcgaagaaaa aatcctcaga
     1861 gaagaacttt acgtggccga cgagatcttt tttacaggca cagccgccga agtcactccg
     1921 gtacgtgaga tagatcatca catgatcggc gatgggaagc ccggccccat caccacacac
     1981 gtgaaggaac ttttctttga tgctgtgcgt ggcaagattg ccaaacacca tgagtggctg
     2041 gaaatcttct gaatagcgaa tcacgaacaa ggaatttcga aatataaatc cctcatttcg
     2101 acattcgaca ttccttgttt cagccatagg actgatcccg ctttgcggga cgctattcga
     2161 tattctaatg cactccccag accgagtcat tattacgagc ctttatttat gaccgataca
     2221 cccgaaaaaa ttattttctc catgattggc gtgggccgca ttcacccacc caaaaaacag
     2281 gtcctgaa
//
LOCUS       DATDUN010000859         1584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_123156, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000859 DATDUN010000000
VERSION     DATDUN010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123156"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VJC18_11905"
     CDS             complement(<1..419)
                     /locus_tag="VJC18_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46128.1"
                     /translation="MSVILKALKRAENDPSPATTDNDSDEGLFADNDGGFFEGKDAFV
                     KKSVLPITSSAISSGGLKTGHILLAGVVLALSFLLTLRYIMNPHLATNVIPNTIEFLP
                     ATNTAQTTTSATTTLATPHQPLPDNLAQQAQVAFEQQ"
     gene            complement(438..1313)
                     /locus_tag="VJC18_11910"
     CDS             complement(438..1313)
                     /locus_tag="VJC18_11910"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLD46129.1"
                     /translation="MYKDFFHFKQDPFNNTPNHHLLFLSLQHEEALESMVYGIKQRKG
                     FIAIIGDIGTGKTTLCRSLLQRIQNHVNTAVIFNPMLSVPELLLAINDDFGLKSSTDR
                     SIKSQIDALNRFLLTRLKQNQNAVVIIDEAQHLTTEAFEMLRMLSNLETEDKKLLQII
                     FVGQRELEGKLASPELKQLNQRISIRYYLKPLNFQETCGYIVHRLALVSQECSVQFEE
                     TALKRVYKHSGGVPRIINNLCDRALLDAYAERQHTITKKTIRRVILDLNGASNPHDSF
                     FFKKIDFSAWRRVFG"
BASE COUNT          390 a          320 c          422 g          452 t
ORIGIN      
        1 tcctgttgtt caaaggcgac ttgtgcttgt tgtgccagat tgtctggtaa aggctgatgc
       61 ggggtggcta gggttgttgt cgcggacgtt gttgtttgcg ccgtattggt ggcaggcaga
      121 aattcgatgg tgttgggaat gacgttcgtg gccaggtggg gattcatgat atagcgcagt
      181 gtcaataaaa acgagagggc aagcacgact ccagcgagca aaatgtgacc ggttttaagg
      241 cctcctgatg agattgccga gcttgtgatc ggcaaaacac tttttttgac gaaagcatct
      301 ttgccctcaa aaaaaccacc atcgttatcc gcgaagagac cttcatcgga atcattgtct
      361 gttgttgcgg gtgaaggatc gttttccgct cttttcaagg cttttaatat gaccgacatg
      421 ttcccccttt gccacaatta cccaaaaact ctgcgccagg cagaaaaatc aattttttta
      481 aagaaaaaac tatcgtgcgg gtttgaagct ccattcaggt ctaaaatgac ccgtcgaatg
      541 gttttttttg tgatggtgtg ttgtctttcg gcgtaggcat ccagcagggc tcggtcacaa
      601 agattgttga tgatccgggg aacgccccca gagtgtttat acacacgctt gagtgcggtt
      661 tcttcaaact ggacagagca ttcctgactg accaaggcaa gtctgtgtac gatgtaacca
      721 caagtttcct gaaaattaag cggcttaaga tagtatctta tggaaattct ttgattgagt
      781 tgcttgagtt ctggcgaggc cagcttgccc tcgagttccc gttgacccac aaaaatgatt
      841 tgcaaaagtt ttttgtcttc cgtttcgaga ttggagagca tcctgagcat ttcgaaggcc
      901 tctgtcgtga ggtgttgcgc ctcatcgatg atgaccacgg cattctggtt ttgtttgaga
      961 cgtgtgagca gaaaacggtt gagcgcgtca atctggcttt tgatggacct gtcggttgaa
     1021 gatttgaggc caaaatcgtc attaatggcc aaaaggagtt caggcacgga caacatggga
     1081 ttgaagatga cagccgtatt gacgtgattt tgaatccttt gcagaagcga gcggcacaaa
     1141 gtcgtcttgc ctgtgccgat gtccccgatg atcgcgatga acccttttct ctgcttgata
     1201 ccgtaaacca tggattccaa ggcctcttcg tgctgaagcg ataaaaaaag aaggtgatga
     1261 ttcggcgtgt tgttgaaggg gtcttgttta aaatgaaaga aatctttgta catggttcac
     1321 gttttcatcg aaggagatgt gtatagccca ccgcccggaa ttgtaaatcg aaagagggtt
     1381 ttgttcaagc atttaatcgg gttaatccac ttatccacag cgagcagggc cttaaggtgg
     1441 ggcaattcca gaaattgttt ttgaagaccc atcctgcata taaaaggtga tcgagaaggt
     1501 cctgccgccc atgttgctat taaagcgcag gtaattgatg gcataggttg tgcctgaggg
     1561 aatggctgtg tccgtgttgt taat
//
LOCUS       DATDUN010000860         3718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_248317, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000860 DATDUN010000000
VERSION     DATDUN010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248317"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VJC18_11915"
     CDS             complement(<1..811)
                     /locus_tag="VJC18_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46130.1"
                     /translation="MPDVQPFPFDELPQCTQAEVVLKKKLFGAYHFFTNRPDFVTLIA
                     QPLVEVLQGSVRLKITHVESKTFEEMGASFSDRSLVGVMRLEPQGKKVGLIFDSLLAK
                     ILIHKILTKQPLTTDKLAQLHIKPLTALEEAVVQYFLVVLIDQITNNINDKNFALSFE
                     DVLRTRDEFRGQFSNQEDYALFSIKLNYLEKDFYLKLVLPLSVSQDLANVEREEEFVN
                     QRIRQFENMKTEFQLEVGYVTLEPSELDSLAAGDIVLFDEMLIEKDQQKISG"
     gene            complement(1072..1434)
                     /locus_tag="VJC18_11920"
     CDS             complement(1072..1434)
                     /locus_tag="VJC18_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006458121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLD46131.1"
                     /translation="MMTIFLCLLLGLLAGVLSGLIGIGGGVIIIPALIFLFGLSQQQA
                     QGTTLALMVPPIGILAAWTYYQRGYVDLKLAALICVGFVVGGLLGAKFATSINSDVLR
                     KIFACSLIVIALKMLLTK"
     gene            complement(1454..2386)
                     /locus_tag="VJC18_11925"
     CDS             complement(1454..2386)
                     /locus_tag="VJC18_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46132.1"
                     /translation="MVDIDNRVDRHEERRTEEKRTEERRQIEKKLQQEPMRTFEAKLS
                     EKTAQELQGKESVAKHMHDLKKSKEDKQSLLDRILGVIKDKSQENEKSRVNAAHRQGK
                     DEQRKETKKRIVEEDFKAKQTREDDKGSSQKTQKKEGEVSQEGHKRVAEKEEGDGGGG
                     FGQGGGRDSQSGERSFDQGAQSKFGEDHKQAEQTKQDAVKGFVGQVREQSRFSSGGFQ
                     RGARNFTQQNLDEIVSAVQLGVNEKGEEEFSVELTDDYFEGLKIMATRTEQGVVVKFI
                     CPNVSVRSTFLKCRPQVYQQLRSKQIEVFRVDVV"
     gene            complement(2394..2888)
                     /locus_tag="VJC18_11930"
     CDS             complement(2394..2888)
                     /locus_tag="VJC18_11930"
                     /inference="COORDINATES: protein motif:HMM:NF028099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46133.1"
                     /translation="MPKKPKYRLNVLLIIKERAKKKAEIALAKAIKKLNEEKEKLKEL
                     EEEKKRIEKRIEEEKNEMRAKVATGQAKMKDPQVCLNFIRKLTEDLEEQEKKIEEQKE
                     EIKKAEKQLQRCRTDYIIAAQEMNMMEQHKELWHKKMNHVLSMEEQKVLNELGNVIHQ
                     MTKK"
     gene            complement(2982..>3718)
                     /locus_tag="VJC18_11935"
     CDS             complement(2982..>3718)
                     /locus_tag="VJC18_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HLD46134.1"
                     /translation="IGERGREVREFIERDLGEEGLKRSVVVCATSDQPSLVRAKAAYV
                     ATAIAEYFRDKGKKVLFMMDSVTRFARALREIGLAVGEPPARQGFTPSVFSTLPKLLE
                     RTGNSDKGSITAFYTILVEGDDMNEPVADEVRSILDGHIVMSRDLANQGHFPAIDVSQ
                     SVSRVMTNIVGEDHVFASRKLKEVVANYEKERDLILIGAYESGSNPAVDYAIEMIDDV
                     NEFLKQGVNDKIDLLESVEQLINLFE"
BASE COUNT          851 a         1051 c          777 g         1039 t
ORIGIN      
        1 aacccgaaat tttttgctgg tctttttcga tgagcatttc atcgaacaac acaatgtccc
       61 cggctgccag cgaatccaat tctgatggtt ccagcgtcac gtaaccaacc tcaagctgaa
      121 attccgtctt catgttttca aactgcctga ttcgttggtt gacaaactct tcttctcttt
      181 ccacgttggc caaatcttgg gatacggata gggggagaac gagtttgagg taaaaatctt
      241 tctccagata attgagtttt atggaaaaca gggcatagtc ctcctgattc gaaaactggc
      301 cccgaaattc atcgcgggtt cgcagaacat cctcgaaaga cagagcgaaa tttttgtcgt
      361 tgatgttgtt tgtaatttga tcgatcagga ccaccagaaa atactgtacc acagcctctt
      421 ccagtgctgt caggggcttg atgtgcagtt gcgccagttt gtccgtggtg agcggctgtt
      481 tggtgaggat tttgtggatg aggattttgg ccagcagact atcaaaaatc agccccacct
      541 tttttccttg cggttcaaga cgcatcaccc ccaccagcga tcggtcagaa aaggaagccc
      601 ccatctcctc gaatgttttt gactccacgt gcgttatctt tagccgcaca ctcccttgca
      661 acacttccac cagaggctgc gcgatcagtg tgacaaaatc cggacggttg gtgaaaaaat
      721 gataggcacc aaaaagcttt ttcttgagga ccacctcagc ctgtgtgcac tgaggcaatt
      781 catcgaaggg aaatggttgg acatctggca taagtcattt gaatgtaatg agttattgac
      841 acagtgtcaa caaattctgg ggcacaaatt cggggcatgg aaatcgatga attgtttttg
      901 aaacgtcgag gacagcacca ccgacaggaa ccattttgcg aaaacagggt atgcaaacct
      961 gattgtctgt ctgacaacag caaaatggtt caacacaaaa caacaaaagg tgccttcgca
     1021 gacgtgaaaa aaacaacgca tcgatttctg tgccccacat caataggggc ctcacttggt
     1081 caacaacatc tttaacgcga tgacaatgag tgaacaggca aagatttttc tgagcacatc
     1141 gctgttgatg ctcgtggcga acttggcacc caacaaaccg ccaaccacaa aacccacgca
     1201 aatgagtgcc gcgagcttca aatccacata gcctcgctga taataagtcc aagccgccag
     1261 aatcccgatc gggggaacca tcagggccag cgtggtcccc tgcgcctgtt gttgtgaaag
     1321 gccaaataaa aaaatcagcg cgggaatgat gatcaccccc ccacctatcc cgatgagacc
     1381 gctcaacacc ccggccagca aacccagtaa cagacaaaga aaaatggtca tcactgtcaa
     1441 ctccttaacc ccctcaaaca acatcaactc tgaatacctc gatttgtttt gacctgagct
     1501 gttgatacac ctgagggcgg catttgagaa acgtgctacg cacgctcacg ttgggacaaa
     1561 tgaatttgac cacgaccccc tgctcggtcc tggtcgccat gattttcagc ccttcaaaat
     1621 aatcatctgt gagttctaca gaaaattcct cctcaccctt ctcgttgact cccaactgca
     1681 ctgccgacac aatttcatcg aggttctgtt gtgtaaaatt tcgggccccg cgctgaaaac
     1741 cgcctgacga aaaccgagat tgttcccgaa cctgtcccac aaaacccttc acagcgtcct
     1801 gtttggtttg ctccgcctgt ttgtggtcct ctccaaattt cgactgcgct ccctggtcaa
     1861 aggatctctc ccctgattgc gaatcccggc cgccaccttg accaaaccca ccgccaccat
     1921 caccctcctc tttttcggct acccgtttgt gaccctcctg agagacctca ccttcttttt
     1981 tttgcgtttt ttgggaactg cctttgtcgt cttccctggt ttgtttggcc ttgaagtcct
     2041 cttcaacaat cctttttttg gtttcttttc gttgctcatc cttgccctga cgatgagcag
     2101 cattcacacg tgatttctcg ttctcctgac ttttatcttt gatcacaccc agaatgcgat
     2161 cgagcaaaga ctgtttgtct tccttgctct ttttgaggtc gtgcatgtgc ttggccactg
     2221 attccttgcc ctgtagttcc tgtgctgttt tttcggagag cttggcctca aaggttctca
     2281 tgggctcctg ctgtagcttc ttttcgatct gacgccgctc ttccgtgcgt ttttcctcag
     2341 tccgccgttc ctcatgccga tcaactctgt tgtcaatatc taccatacac acctcatttc
     2401 ttggtcattt gatgaatcac gttacccaac tcgttgagca ctttttgttc ctccattgac
     2461 aagacgtgat tcattttttt gtgccacaac tctttatgct gttccatcat gttcatttcc
     2521 tgtgcggcga tgatgtaatc agtacgacac ctctgcaact gcttttcggc cttcttgatc
     2581 tcttccttct gctcctcgat ttttttttcc tgttcttcca ggtcctcagt gagcttgcga
     2641 ataaaattga gacacacctg cgggtccttc attttggctt gcccggtagc caccttggca
     2701 cgcatctcat ttttttcttc ctcaatgcgc ttctcgatcc gttttttttc ctcctccaat
     2761 tccttgagtt tttccttttc ttcgttaagt tttttgatcg ccttggccag cgcgatctcg
     2821 gcctttttct tggccctctc tttgatgatc agcaggacat tcaatcggta ttttggtttc
     2881 tttggcataa atcactgtcg actgccaaca gccaacggtt aaccgatgtc cggctgtagg
     2941 ttgtagaccg ttggccgtca gccctgactt ctcaactcga ttcactcaaa cagattgatc
     3001 agttgttcca ccgattccaa cagatcaatc ttatcattca caccctgttt caaaaactca
     3061 ttcacgtcat cgatcatttc gatcgcgtaa tccacagccg ggttgctgcc cgattcgtag
     3121 gccccgatca aaatcaggtc ccgttctttt tcgtaattgg ccaccacctc tttgagtttg
     3181 cgcgacgcga acacatgatc ttctcccaca atgttggtca tcacgcgtga cacggattgc
     3241 gacacatcga tcgctggaaa atgcccctga ttggccaaat cgcggctcat cacgatatgc
     3301 ccgtccagga tggacctcac ctcgtccgcc acaggctcgt tcatatcatc accctcaacc
     3361 agaatcgtat aaaacgccgt gatggaacct ttgtctgaat tccccgtccg ttcaagcagt
     3421 ttgggcaacg tggaaaacac agacggcgtg aacccctgtc tggcgggtgg ttcccccacg
     3481 gccagaccga tttcgcgcaa ggccctggcg aagcgggtga ccgagtccat catgaataaa
     3541 acttttttgc ctttatcacg aaaatactcg gcgatggcag tggccacgta cgccgctttg
     3601 gcgcgaacga gggacggctg gtcggacgtt gcacacacca ccacagaacg tttgaggcct
     3661 tcttccccca aatctctctc gatgaactca cgcacctcgc gtccacgctc accgatca
//
LOCUS       DATDUN010000861         1418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_538795, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000861 DATDUN010000000
VERSION     DATDUN010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538795"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1418
                     /locus_tag="VJC18_11940"
     CDS             <1..>1418
                     /locus_tag="VJC18_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLD46135.1"
                     /translation="ATQVAHDIRSPLSALHVATTDLRKLPEEQRILIRTAVQRITDIT
                     NDLSNQKRKAQNAQLEGAENLTNEKDEAETGQKLADNDSVQLLSAVIESLVSEKRMQF
                     RNRSGINIEANLGKESYGLFAKIQPKAFKRLLSNLINNSVEAMEGGGSVVVTMRTVEP
                     QSRRAIEGQNPSVTEKTDSGFTVVPPLHGFTAQDIQITISDTGQGIPADVLPRLFQRG
                     ETYGKKTGSGLGLYHARQTIEAFGGSITIQSETGQGTVVTIKLPQIVAPEWFLEKIVV
                     HQNMTIVICDDDESIHHVWQNQLNNLLEPVKNVVHIRTPEALGEWFWGKRRNEGRKHE
                     GRGDLGSAKCKVQSAKCKMASDMLFLCDYEFLGSEKTGLDVIEELGIGGQAILVTSHY
                     EEDAIRTNCARLGVKLIPKSLAGFVPIKRISSKLYPSTGLGQEAEPFDGLRARSSKGN
                     VEDGRACLASQRTRDPAIPIVP"
BASE COUNT          405 a          324 c          388 g          301 t
ORIGIN      
        1 tggccaccca ggtcgcccac gacatccgtt caccgctgtc tgcgttgcat gtggccacca
       61 cggatttgcg gaaattgccg gaagaacaac gcattcttat tcgcaccgcc gtgcaaagga
      121 taacagatat caccaacgat ctttctaacc agaagcggaa ggctcaaaac gcccagctcg
      181 aaggtgcaga aaatttaacc aacgaaaagg atgaggcgga aacgggacaa aagttagcgg
      241 acaacgattc tgtccagttg ctttcggctg ttattgagtc actggtatca gaaaaacgca
      301 tgcagtttcg caatcgctca ggcataaata tcgaagccaa tcttggaaaa gaatcatatg
      361 gtctttttgc caaaatacaa ccgaaagcct ttaagcggtt attgtcaaat ctcatcaaca
      421 actctgtgga ggcgatggaa ggaggcggga gtgtggtggt tactatgaga accgtagaac
      481 cgcagagccg tagagccata gaaggacaga accctagcgt tacggaaaaa actgattctg
      541 gattcactgt ggtgccaccg ctccatggtt tcacggctca agatattcaa atcaccatct
      601 ccgacacggg ccagggcatc ccagcggatg ttctgccgag gctttttcaa cgcggcgaaa
      661 catatggcaa gaagacgggg tctggtttgg gactatacca tgccaggcag accattgaag
      721 cctttggcgg cagtatcacc attcaatcag aaacagggca aggcactgtc gttacgatca
      781 agttgccaca gatcgtagcc ccggaatggt ttttggaaaa aatcgttgtg catcaaaaca
      841 tgacgattgt gatttgtgat gacgatgagt ctatccacca tgtatggcaa aatcagctga
      901 acaacctcct tgaacccgtg aaaaatgtgg tgcacatcag gacgcccgag gcgttggggg
      961 aatggttctg gggtaaacga agaaacgagg gacgaaaaca cgagggacga ggggatttgg
     1021 gaagtgcaaa atgcaaagtg caaagtgcaa aatgtaaaat ggcaagcgat atgctgttct
     1081 tgtgtgatta cgaatttctg ggcagtgaaa agaccggtct cgatgtcatt gaggaactgg
     1141 ggattggcgg tcaggcgatt ctggtcacca gtcattatga ggaagatgcg attcgcacga
     1201 attgtgctcg attaggcgtg aagttgatac ccaaaagtct ggcggggttt gtgccgatca
     1261 aacgaatcag ctcaaagctg tacccttcga cgggcttagg gcaagaagct gaacccttcg
     1321 acgggctcag ggcaagaagc tcaaagggaa acgtagaaga tggccgtgcc tgccttgcga
     1381 gccagcgaac ccgcgatcct gcgatcccca tcgtgcca
//
LOCUS       DATDUN010000862         5604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_414284, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000862 DATDUN010000000
VERSION     DATDUN010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414284"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1326)
                     /locus_tag="VJC18_11945"
     CDS             complement(103..1326)
                     /locus_tag="VJC18_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLD46136.1"
                     /translation="MPTFSYRARNDDGQLMTGQIDAATEKDAKIMLVGQGLIPVSLET
                     TATEVRLPLFWRKSRKAQTKELIALTRQFAALFKAGVSMDRILYSLIKQTQNIDLKNT
                     MMQIQEDVSHGASLMTAFAKHPKYFNDLYVNMINVGEQGGVLDHTLRTLVDILKKEYR
                     IINSIKSATLYPKIVLITFLVVVTLLMMFVMPGFADFYAGFDAKLPLPTIIMMTISDF
                     FVDYWYVLLAIAITLYLAYKKYSATEKGSFQIDGLKFRLPVFGHLNLMVVNARFGHLI
                     ASLYSSGLTLGRALTIVGNTIGNKVYKKDIDMVRESMNQGASLSDAMKNSKRFTSLMI
                     ESVSTGEQSGSLDEVLNTTAEFYDEEVTDILDQLTTLIEPILLFGLFGMIALLAFAVF
                     LPIWKTTQLILPPLQ"
     gene            complement(1343..3019)
                     /locus_tag="VJC18_11950"
     CDS             complement(1343..3019)
                     /locus_tag="VJC18_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HLD46137.1"
                     /translation="MYTLEDILVSAGLVTPDQMLIAKESQKNLGDPLEQVLIERGFIN
                     ETLLLKTIAKHMNTSYVSLGRYKLDPQVLGLLPVDAIKKFKAIPLFKIENKVTVAMSN
                     PADLDALDSIRMLLKCDVEPFFALDHDIERVIKEQFRGQGITLKEIETKIQLMTTEDE
                     VVPNTQTEKLAKEASGEAVVNAVNNILLQALEDGASDIHLEPSRRGLLVRLRLDGLLE
                     EFTTLPKNIHLPVISRVKIMSHIDVAESRLPQDGRFRFRVGETETDVRVATYPTMFGE
                     AAAIRLLSKGQLITLEQLGFQPEDMLKFAGLIAKPHGILLVTGPTGSGKTTSLYASLL
                     RINSKDKHILSIEDPVEHEIEGIDQQQVNVKAGMTFAAALRAMLRQDPDVIMVGEIRD
                     LETADIAMRAAMTGHLVLSTLHTNTAVGAISRLMDLGIEPYLVSSSLIGVLAQRLVRR
                     ICHHCKEGTVIPQDQLKRLGKNIAIEKAFKGAGCKFCRNTGYSGRVGLFEIVVVTEEI
                     KNLIVAKAPEALLMEKLLELGHKTIIHDGLKKANQGLTTVEEILRVTASI"
     gene            complement(3068..4039)
                     /gene="pdxA"
                     /locus_tag="VJC18_11955"
     CDS             complement(3068..4039)
                     /gene="pdxA"
                     /locus_tag="VJC18_11955"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLD46138.1"
                     /translation="MSKFCPANNIAVTLGDPLGIGPEVCFKALLSPRLQQTTRLTLIG
                     PLGLFATDPRLKELRQKKNILLVDTAAGPLSRLRMSPKTAGLHAFLALKKAVALIKNK
                     QVCALVTGPISKENVKLAGFAFPGHTEFLCTTFNVSDYAMMLFNDKLRVVLTTIHIPL
                     KKVSPSLTKQSIVTKLALTARSLRHLFGIPHPTIAVCGLNPHAGENSLFGQEEKTIIT
                     PAIKQFLKEKLNKHTAVLGPLSADTVFASAIKGQFDAVLCHYHDQGLIPIKLMGLEHA
                     VNMTLGLPFVRTSPAHGTAPDIAGRNLADPSSMLAALNAAVAFTQGS"
     gene            complement(4017..4970)
                     /locus_tag="VJC18_11960"
     CDS             complement(4017..4970)
                     /locus_tag="VJC18_11960"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HLD46139.1"
                     /translation="MKKTFLFTLLCCLFTLSATAMTIDRIVAKVGSEVITLSDLKQEG
                     AERKAYLIHTQGPQKGAEAFARFSKNLLDELVLEKILESEISKNSIEISDAQVEQELQ
                     AQRNRKQLDEKAFGEALSRSGLTLAEYKKRLRLDLEKQMFIQKKIAPLINVSEYDLQQ
                     EYQKNILNYQTYRKLRFIEAFLTIDTFPDQQKMLETANNIQAALKAGQTPAQLIKKHS
                     SGAFAQNGGDSGLVESSSLRPEVLSILDSLKNGETSEQLTFPGGILVFKLISKAEPQP
                     LPFNQVVNQVRMRYFESIVDSELKKYLLSVKDKTYVEILSR"
     gene            complement(4967..>5604)
                     /locus_tag="VJC18_11965"
     CDS             complement(4967..>5604)
                     /locus_tag="VJC18_11965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLD46140.1"
                     /translation="ERKKRVDTKTLDTLLLEHNIPYSRWKQIVENEIKVQYVLDQEFA
                     KDLVISASEIRSYYTQNEGSFVVPEKVRVRHIVTDTLEKAHEVHERLLKGENFAKMAV
                     NHSQSPDRTKGGDLGYFSRGNFPKEFDDTCFGLQKGEISPIVKSDYGYHIFKLLDRQP
                     PGRTSLLEASSVIYQKLFEEKLNKKYGEWITHARTEVTINTNTETLKGFFL"
BASE COUNT         1299 a         1260 c         1519 g         1526 t
ORIGIN      
        1 ctcttcgcaa gcggggcttg ctccgcaggc cgcatcaaaa taatagctcc ccgtatcacc
       61 ccaaaaatca aaacgctcca atgagtgacc gattacggaa agtcattgga gggggggcag
      121 gatcaattgt gtggtcttcc agatgggcag aaaaacagca aaggccaaca gcgcgatcat
      181 gccaaacagc ccgaacaaaa gaatgggctc aatgagtgtg gtgagttgat ccaggatgtc
      241 tgtgacctct tcgtcataaa attccgcggt ggtgttcaag acttcatcga gagatccgga
      301 ctgttcgccc gtactcacag attcaatcat gagggaagtg aaccttttgg aattcttcat
      361 ggcgtccgaa agagaagccc cctgattcat ggactctctc accatatcga tgtctttttt
      421 gtagaccttg ttgccaatgg tgttgcccac gattgtgagt gcgcgaccca aggtcaaacc
      481 actgctatag agtgacgcga tcaaatggcc gaaccgcgcg ttaaccacca tcaggttgag
      541 gtggccaaag acgggaagcc tgaattttaa accatcaatt tgaaaactgc ctttttccgt
      601 cgcagagtat tttttatagg caaggtacag ggtgatggcg atcgccagca agacgtacca
      661 gtaatctaca aaaaaatcac tgatggtcat catgatgatg gtggggagag gaagcttggc
      721 gtcaaaaccg gcatagaagt cggcaaaccc cggcatgaca aacatcatga gcaaggtcac
      781 gacgacgagg aaggtgatga ggacaatttt gggatacagt gtggcggact tgatgctgtt
      841 gatgatgcgg tattcctttt tgaggatgtc aaccagcgtg cgtaaggtgt ggtccagcac
      901 gccgccctgt tctcctacgt tgatcatatt gacgtacaga tcattgaaat attttgggtg
      961 ttttgcgaag gcggtcatga ggctggcgcc gtgtgaaacg tcttcctgga tttgcatcat
     1021 ggtgtttttg agatcgatgt tctgcgtttg tttgatgagg gaatataata ttcggtccat
     1081 ggacacaccc gccttgaaca aggccgcgaa ctgtcgtgtg agggcgatga gctccttggt
     1141 ctgtgccttg cgactctttc tccaaaacaa ggggagtctc acctccgttg cggttgtttc
     1201 aaggctgaca gggatgagac cctgccccac cagcatgatt tttgcgtctt tctcggtcgc
     1261 agcatcgatc tgaccggtca tcaattggcc atcatcattt ctggctctgt acgaaaaggt
     1321 tggcatgagt aataggctgc ccttaaattg aggccgtcac gcgcagaatt tcttctacgg
     1381 tcgtgagtcc ttggttcgct tttttcaggc catcatgtat gattgtcttg tgtcccaact
     1441 caaggagttt ttccataagg agtgcctcag gggcctttgc gacgatgagg tttttgatct
     1501 cctctgtgac caccacgatt tcgaacagcc ccacacgacc tgaatagcct gtgttgcggc
     1561 agaatttgca accagccccc ttgaaagcct tttcgatcgc gatgtttttt ccaagtcttt
     1621 tgagctggtc ctgaggaata actgttcctt ctttgcaatg atggcaaatg cgtcgcacca
     1681 agcgttgtgc cagcactcca atgagactgg aactgaccag gtaaggctcg atgcccaaat
     1741 ccatgaggcg cgagatggcg ccgactgccg tgttggtgtg aagggttgat aacaccaaat
     1801 ggcctgtcat ggcggcacgc atggcaatat cggcagtttc gagatcgcgt atttcaccca
     1861 ccatgatcac gtcgggatcc tgtcggagca tggcgcgcaa agccgcggca aaagtcatac
     1921 cggccttgac gttaacctgt tgctgatcga ttccctctat ttcgtgctcc acggggtctt
     1981 caatggacag gatgtgtttg tctttcgaat tgattcgcaa caaagacgcg tagagcgatg
     2041 tggttttgcc tgagcccgtt ggtcccgtga cgagcagaat gccgtgtggt ttggcgatga
     2101 gaccggcaaa tttgagcatg tcttctggct gaaaaccaag ctgttccaaa gtgatgagtt
     2161 ggcctttgct gagaaggcgg atggctgcgg cctcgccgaa catcgtggga taggtcgcca
     2221 cacgaacgtc agtttctgtt tctcccaccc gaaaacgaaa tcgcccatcc tgtggcagac
     2281 gcgattctgc cacatcgata tggctcatga ttttgacgcg actgatgacg ggtaaatgaa
     2341 tgtttttggg tagtgttgtg aattcttcca acagaccgtc gagtctgagc cgcaccaaca
     2401 accctctgcg ggatggttcc aggtgaatgt cgctggcgcc gtcttcgagg gcttgcagca
     2461 ggatgttgtt gacggcgttc acgacagcct caccggaggc ctcttttgcg agcttttcgg
     2521 tttgtgtgtt gggcaccact tcgtcttcgg tggtcatgag ttggatcttt gtttcaattt
     2581 ccttgagggt gatgccctgg ccgcgaaatt gttccttgat gacgcgttca atgtcgtggt
     2641 caagtgcaaa gaaaggttct acatcgcact tgagcaacat ccgaatgctg tcgagcgcat
     2701 cgagatcagc cgggttggac atggcgacgg tgaccttgtt ttcgatcttg aacaagggga
     2761 tggccttgaa ttttttgatg gcatcaaccg gtaacaatcc cagcacttgt gggtccaact
     2821 tgtatcgccc caatgaaacg tacgatgtgt tcatgtgctt ggcaattgtt ttgagaagca
     2881 gggtttcgtt aataaacccg cgttcgatca agacctgttc caggggatcg cccagatttt
     2941 tttgcgactc ttttgcgatg agcatttgat cgggggtaac gagaccggca ctgactaaaa
     3001 tatcttctaa tgtgtacatg aaaaggattc cttgccaaat ttgagggggc ttcctgtggg
     3061 catcatatta ggagccttgt gtaaaagcaa ctgccgcgtt gagggcggcg agcatgctgg
     3121 aaggatcggc caaatttctg ccagcaatgt caggtgcggt gccatgtgca ggagaagtgc
     3181 gcacgaaagg aaggcccaga gtcatgttga cagcatgctc caggcccatg agcttgatcg
     3241 ggataaggcc ttggtcgtga tagtgacaga gcacggcatc aaactgtcct ttgatggctg
     3301 atgcaaaaac agtgtcggca gaaagtggcc cgaggacagc agtgtgtttg tttagcttct
     3361 ctttgagaaa ctgtttgatg gctggggtga tgattgtttt ttcttcttgt ccgaaaagcg
     3421 aattttcacc ggcatgtggg ttgaggccgc aaacagcaat tgtggggtgt ggtatgccaa
     3481 acaggtgcct gagagaacgg gccgtcaagg caagttttgt cacgattgat tgtttcgtga
     3541 gactggggga cacttttttg aggggaatgt gaattgtggt caatacaaca cgtaatttgt
     3601 cattgaacaa catcatggca taatcactga cgttgaaagt tgtacataaa aattcggtgt
     3661 gtcccggaaa agcaaagccg gccaatttga cattttcttt tgaaatgggc cctgtgacga
     3721 gagcgcagac ctgtttgttc ttgatgaggg caacggcttt ttttagcgcc aggaaagcgt
     3781 gtaagccggc tgtcttgggg gacatgcgga gacggctcaa aggaccagcc gcggtatcga
     3841 ccagaaggat gttctttttt tgtcgaagct ctttcaaacg agggtcggtg gcaaacagcc
     3901 ccagggggcc gatgagagtc aaacgggtag tctgttggag gcgtggacta agcagtgcct
     3961 tgaaacacac ttctggtccg attcccaacg gatcgcccag tgtcacagca atgttgttag
     4021 cgggacaaaa tttcgacata ggttttgtct ttgactgaga gcagatattt cttgagttcg
     4081 gaatcgacga tggattcgaa gtaccgcatg cgaacctggt tgacaacttg gttaaaggga
     4141 agcggttgtg gttcggcttt ggaaatgagt ttgaacacaa gaatcccgcc cgggaaggtg
     4201 agctgctcac tggtttcacc gtttttcaag gaatccagaa tggacaacac ttcaggtcgc
     4261 agagaggaac tctccaccaa accggaatca ccaccgtttt gtgcgaacgc cccagaagaa
     4321 tgttttttga tgagctgtgc aggtgtttga cccgctttga gggcggcctg tatgttgttg
     4381 gctgtttcca gcatcttctg ctggtctgga aatgtgtcga tcgtcaagaa ggcttcgatg
     4441 aaacgcagtt tgcgataggt ttgataattc agaatgtttt tctggtactc ctgctgcaaa
     4501 tcgtattctg aaacattgat caacggggcg atttttttct gaatgaacat ttgtttctcg
     4561 aggtctagac gcaggcgttt tttgtattcg gcaagagtga gtccggagcg gctcagggcc
     4621 tcgccaaaag ccttttcgtc caactgtttt ctgttgcgct gggcctgaag ctcctgttcg
     4681 acctgcgcgt ccgagatctc gatactgttt ttggaaattt ccgattcaag aattttttcc
     4741 aaaacgagtt cgtccagaag atttttactg aacctggcga aggcctcagc ccctttttgt
     4801 gggccctggg tatgaatcag ataggccttt ctttcggccc cctcctgttt gagatcgctg
     4861 agcgtgatga cctcggagcc aactttggcc acaatgcggt caatggtcat ggccgtcgcg
     4921 ctgagggtaa aaagacaaca gagtaaagtg aagagaaaag tttttttcat aaaaagaaac
     4981 ctttaagggt ttcggtattg gtgttgatgg tgacttctgt tcgtgcgtgc gtgatccact
     5041 caccatattt tttattaagc ttttcctcaa acaatttctg ataaattaca gaggaggctt
     5101 ccagcaaaga agttcgtccg ggaggctgtc tgtctaagag tttaaagatg tgatacccgt
     5161 aatcactctt gacgattggg ctgatctcac ccttttgcag gccaaagcag gtgtcgtcga
     5221 attctttggg aaaatttcct ctgctaaaat accccagatc accacctttt gtgcgatctg
     5281 gtgactggga atggttgacc gccattttgg cgaaattctc acctttgagg agtctttcgt
     5341 gcacctcatg tgccttttcg agggtgtcag tgacaatgtg tctgacacga accttttcgg
     5401 gaacaacaaa ggacccctca ttttgggtgt agtatgagcg gatctcgctg gcgctgatca
     5461 ccaggtcttt ggcgaattcc tggtctagaa catattggac cttgatttcg ttctcaacta
     5521 tctgtttcca acggctgtaa gggatattgt gttctaataa caaagtgtcg agcgttttgg
     5581 tatcaacgcg tttttttctc tcgt
//
LOCUS       DATDUN010000863         1802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_455484, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000863 DATDUN010000000
VERSION     DATDUN010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455484"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1677
                     /locus_tag="VJC18_11970"
     CDS             388..1677
                     /locus_tag="VJC18_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46141.1"
                     /translation="MVIKASGGYVSFDFLGSASGTNIVLSDVAKAARQQQLSDMLAPG
                     LGGQTPEGAVRARSQLLKDKYGLRGVVTKPSSVVRNQLPATLSAGTHTSRISRRFGAS
                     ASMSAGDETTGKSPPSQSRPVISPSSVSPAAKIEDALKVLWKEVPLFISIGNDGVVAQ
                     TSEEAATGQRAIKMWWTQRALAPAQWHIKNVGNAPVFIAALEQWNNRIDTKTGPSLDS
                     GQEATLNRACHYVELRVFCNVSTSSIKIPKYVSGQKVRMYRSGGGYVVWQVGQQWGDH
                     VKVYDPHDSSKYAIVSKIHLDQMQNGTYDPNKRDVEMIGTETMSFILKNFPPAPEGTQ
                     NPPPRLPPPAIAINELQRPGDVLRGDKLKAYYWLACRLAQSDDNDPRMQELLAILIRI
                     DPLQKLLNDPSINEGVKDELAQGKYENIFAQLIKQGD"
BASE COUNT          501 a          385 c          456 g          460 t
ORIGIN      
        1 ctctgactca aactgcggta ggaatatttt tgtgggtcct gcttttttcg ggtttcgtaa
       61 aaatcagtga gaaaatgccg ataatcatcg taggaaaaaa catttaattc gtttccattt
      121 ttttcttcgg tcataatgag tgccctgcaa tgctgttgtc ttttcgctta tcgaatctat
      181 gtccgataat gttgctagga aacgaagatg tttttggggc aataaaaagt tagcaaatac
      241 agggtgttgg atgttgttta tttgctgtgc accgactttt tgtatcggtt cttgtttccc
      301 caaggaaacg gtttttgtta tttgtgcgaa acaacaagac tcgtttaccg ataagtcgcc
      361 aaaggaatat atcagaaagg aaaaattatg gttataaaag catcgggtgg ttatgtcagt
      421 tttgattttt tgggtagcgc ctctggaacc aatatcgttt tgtcggatgt ggctaaagcg
      481 gccagacaac aacagctgtc agatatgctg gccccggggc tgggagggca aacgcctgaa
      541 ggcgctgttc gtgcaagaag tcagcttctt aaggataaat atggcttaag gggcgttgtc
      601 acaaagccat catccgttgt ccgcaaccag ttgccagcaa cattgtctgc gggcacacac
      661 acaagtagga tcagtcgcag atttggtgcc tccgcaagca tgtcggcagg ggacgaaaca
      721 acagggaaga gtccaccgtc tcagtcgagg cctgtaataa gcccctcttc ggtgagtccc
      781 gccgcaaaaa ttgaagacgc actgaaagtt ttgtggaaag aagtccccct attcatttct
      841 ataggcaacg acggtgtcgt tgcccaaaca tcagaggagg cggccacagg tcaaagagcg
      901 attaaaatgt ggtggacaca gagggcttta gcacccgctc aatggcatat caaaaatgtg
      961 ggcaacgccc cggttttcat agccgccctg gaacaatgga acaacaggat cgacacgaaa
     1021 actggcccat cattagattc aggccaagaa gcaaccctga accgagcttg tcattatgtt
     1081 gagctgaggg ttttctgcaa tgtgtcaaca agttctatta aaataccaaa gtatgtctcg
     1141 ggtcaaaagg tcagaatgta caggagtggt ggagggtatg tggtgtggca agttggacag
     1201 caatggggtg atcatgtgaa ggtctacgat ccacatgata gctccaagta tgccatcgtc
     1261 agtaagattc acttagatca aatgcagaat ggtacatatg atccaaataa gcgcgatgtt
     1321 gaaatgattg gcacagagac gatgagtttt atattaaaga attttccgcc tgccccagag
     1381 ggcacacaaa atccgccgcc acgattgccg ccaccagcaa tagcaatcaa tgagcttcaa
     1441 cgtcccggtg atgttttgcg gggagacaaa ctcaaggcct attactggct tgcttgtagg
     1501 cttgcccaga gtgatgacaa cgatccccgc atgcaggagt tgctggcgat attgatacga
     1561 atcgatcccc tacaaaaatt attgaatgat ccgagtatta acgaaggtgt gaaggatgag
     1621 ctggcacaag gaaaatatga aaatatattc gcacagttga taaagcaggg cgattagttt
     1681 ttcgctgttt atgccgattc gcctagctcc tcaaaaatcc gggtcaatgc ccagccgccc
     1741 tgatggaccg cgtgtgcgcg ccagtcaata tcgtgcgggt taaggagctt catcacccca
     1801 ct
//
LOCUS       DATDUN010000864         1138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_538859, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000864 DATDUN010000000
VERSION     DATDUN010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538859"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1138)
                     /gene="ggt"
                     /locus_tag="VJC18_11975"
     CDS             complement(<1..>1138)
                     /gene="ggt"
                     /locus_tag="VJC18_11975"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLD46142.1"
                     /translation="LAQRGFIVSAELSRLLTKYGTELKRHPSTAKIFFHPDGTAYQAG
                     ERLIQQDLANSLLKIASEGPQTFYRGPLAKLISDHILSQGGLITPSDLRNYQTVIRKP
                     VRGTYRGFDVYSMPPPSSGGVHLIQMLNMLEPYSLSRMIPNSVDWIHLVVGVMQRAYA
                     DRSVHLGDPSFVQNPVTGLTSKGYARKQMRNFDIDHALSSQQIKPGIPLQAHEGDQTT
                     HFSVIDKDGNAVSNTYTLNTNFGMKAVVPGTGILLNNEMDDFTTKEGAQNVYGLVQGA
                     KNRIEPGKRMLSSMTPTIILKDKKPFLITGTPGGSRIITTVLNIILNVIDSKQSIVTA
                     TKAPRFHHQWLPDEIVVEPGFAPQTRTKLKTKGHHLKPSPPYCCA"
BASE COUNT          256 a          246 c          336 g          300 t
ORIGIN      
        1 tggcacagca gtagggagga gaaggtttga ggtgatggcc tttggttttg agtttggtgc
       61 gtgtttgtgg agcaaatcca ggctcaacga caatttcgtc cggcaaccat tggtggtgaa
      121 agcgaggggc cttggtagca gtgacaatac tttgttttga gtcaatgacg ttcaaaatga
      181 tgttcaaaac agtcgtgata atgcggctac cgccgggcgt gcctgtgatc aaaaagggtt
      241 ttttgtcttt tagaatgatc gtgggggtca tggaactcag catgcgtttg ccgggttcaa
      301 ttctattttt ggccccctgt accagcccgt acacattctg cgcgccttcc ttggtggtga
      361 aatcatccat ctcgttgttg agcaaaatcc cggtgccggg gaccacagcc ttcatcccaa
      421 aattggtgtt gagtgtgtac gtgttggaga cggcattccc atctttgtcg atgactgaaa
      481 aatgtgtggt ctgatctcct tcgtgggcct gcaagggaat acctggtttg atctgttgcg
      541 aagacagggc gtggtcaata tcaaaatttc gcatctgttt tctggcatag cccttggacg
      601 tgaggcccgt aacgggattt tgtacaaatg agggatcacc cagatgcacg gaacgatcgg
      661 cgtaagcgcg ttgcatgacg cccacgacga ggtgaatcca atcaacactg tttggtatca
      721 tgcgggaaag agaataaggc tcgagcatgt tgagcatctg gatcaggtgc acgccgcctg
      781 aactgggagg aggcatggaa tacacatcga agccgcgata cgtgcccctg acgggtttgc
      841 ggataacggt ctggtagttt cgcagatcac ttggggtgat caggccacct tgcgagagaa
      901 tgtggtcgga gatcagtttg gccagcgggc ctcgataaaa ggtctgtggt ccctcacttg
      961 cgattttcaa aagtgagttg gccaggtctt gctgtatcag gcgctcaccg gcctgatagg
     1021 ccgtgccatc gggatgaaaa aatattttgg ctgtggatgg atgtcttttg agttccgttc
     1081 catactttgt gagcagtcgc gatagctcgg ctgaaacaat aaaacctctc tgagcgag
//
LOCUS       DATDUN010000865         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_414425, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000865 DATDUN010000000
VERSION     DATDUN010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414425"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VJC18_11980"
     CDS             complement(<1..524)
                     /locus_tag="VJC18_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLD46143.1"
                     /translation="MENNKDHSIPLITFKNVFKSFDDKPVLEDISFEVYEGETFCIVG
                     GSGTGKSVTLKLLMGLMPVDSGEIYFKGQEITSLSERELNEMRLNFGMVFQSSALFDS
                     MSVFDNVAYPIRELKKYSEDEIEKMVLEKLEVVGLVEAAELFPADLSGGMRKRIGLAR
                     AIACNAPVIIYDEPT"
     gene            complement(688..1218)
                     /locus_tag="VJC18_11985"
     CDS             complement(688..1218)
                     /locus_tag="VJC18_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HLD46144.1"
                     /translation="MPSTKLVTLPHVEKVIFFIRGEKVILDTDLATLYGVKTKVLNQA
                     IKRNRHRFPDDFLFQLSMPEKIELVTICDRFSKLKHSTSLPYAFTEYGAIMAATVLNT
                     KLAIEVSVFVIRAFVKLRETLSAHTQIAKKLNLLENKVVNHDVQIKALFDAIRALMEL
                     PQKKKRMIGFEREKEF"
     gene            complement(1242..2078)
                     /locus_tag="VJC18_11990"
     CDS             complement(1242..2078)
                     /locus_tag="VJC18_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLD46145.1"
                     /translation="MTSAPHNLPSLPPNENVQESVRSLLQRWGAVVWDFLDYAGGLCL
                     LAGRTVQALFLYPLDLRYVIRQLYEIGVKTLTLSSLVAIFTGMVMALQLIVGLKRFGL
                     ELYTGQIVGVAITRELGPVLTALMVASRVGSGIAAEIGSMEVSEQILAIRAMGGSPIA
                     KLVLPRILVTTLAAPMLTIIAIFIGIVGGMFVTMTEAGMTSRFYFDQIQSTVQIYDFL
                     SGVGKTVFFGFFIGCIACYQGLNTKKGTKGVGDATTKTVVICSIVIFISDFFLTKLFL
                     LF"
     gene            complement(2075..>2424)
                     /locus_tag="VJC18_11995"
     CDS             complement(2075..>2424)
                     /locus_tag="VJC18_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46146.1"
                     /translation="FLNKNDASTITIDSWCQRAAADGSLEGLTQDLLRGLADVQIQQT
                     QMHNTHGRAALLTEAQGDMDGREIFMSTFVFKMNACVFDFVYVTLPGELLYYQDFKNM
                     IDGFAFIKGPKLL"
BASE COUNT          675 a          646 c          512 g          591 t
ORIGIN      
        1 gtgggctcgt cgtagatgat caccggcgcg ttgcaggcaa tcgcccgagc gaggccaatt
       61 cttttacgca tcccaccgga aaggtccgct ggaaaaagct ccgccgcttc gaccagcccc
      121 accacctcga gtttttccag aaccattttt tcgatctcgt cttcggaata ttttttgagt
      181 tccctgatgg ggtacgccac gttgtcaaac acgctcatcg aatcgaacag ggccgagctt
      241 tgaaacacca tgccaaaatt aagcctcatt tcgttcaatt cccgttcact caagctggtg
      301 atctcttgcc ccttaaaata aatttcaccg gaatccaccg gcataagtcc catcaaaagc
      361 ttgagggtca cgcttttgcc cgtgcccgat ccgcccacaa tgcaaaaggt ctcaccctca
      421 tacacctcaa agctgatgtc ctccaacaca ggcttatcgt cgaacgattt gaacacgttt
      481 ttgaaagtga tgaggggaat gctgtgatct ttattatttt ccatatttgt attcaatgca
      541 agctcacacc ccaccccctc cacaatcttg atttcatctt cagcaaataa tagcaaccac
      601 acattcctct tcaagccgcc aatgacaaga ttccaatttc gcggacactg tccgcaattt
      661 cttggacaat ttggctgatc ctggaaatta aaattctttt tctctctcaa acccaatcat
      721 cctttttttc ttttggggaa gttccatcaa ggctcgtatg gcatcaaaaa gggcttttat
      781 ctgaacgtca tgattaacga ctttattttc aagcaggttc aattttttgg caatctgtgt
      841 atgggcggac aatgtttctc tgagttttac aaaagccctg atcacaaaaa cactcacctc
      901 aatagcaagc ttggtgttca aaaccgttgc tgccataatg gcaccatatt cagtaaaagc
      961 atacggaaga cttgtagaat gtttgagttt agaaaaccgg tcacagattg tgaccagttc
     1021 aattttttcc ggcattgaga gttgaaaaag aaaatcatca ggaaagcgat gcctattgcg
     1081 tttaatggcc tgatttaaga cttttgtttt aacaccatac aaagttgcca aatcagtatc
     1141 caggatcact ttttcgcccc tgataaaaaa gataaccttt tccacatgcg gtaatgtaac
     1201 aagttttgtt gatggcataa aattttatat ctgaacaggt cctaaaacaa caaaaacaac
     1261 ttggttaaga aaaaatccga aatgaaaatc acgatcgagc aaatcaccac cgtcttggtg
     1321 gtggcatcac ccacaccctt ggtgcccttt ttggtattga gcccctggta acaggcaatg
     1381 caaccgatga aaaaaccaaa gaacactgtc ttgccgacgc cgctcaaaaa gtcgtaaatt
     1441 tgcacagtgg attggatctg atcaaaataa aatcgcgagg tcatgcccgc ctcggtcatc
     1501 gtcacgaaca tcccccccac gatgccaatg aaaatggcaa tgatcgtgag catgggcgcg
     1561 gccagcgtgg tgacgaggat tcgcggcagt acgagtttgg cgatgggact ccctcccatg
     1621 gcgcggatcg ccagaatttg ctccgaaacc tccatcgagc caatctcagc cgcaatgcca
     1681 gaacccacgc gcgacgccac catgagtgca gtcaacacag gccctagctc gcgagtgatg
     1741 gccaccccca cgatctgccc ggtatacagt tcgagcccaa aacgtttgag accgacaatc
     1801 aactgcaagg ccatgaccat cccggtaaag atggccacca aactcgacag cgtcagcgtc
     1861 ttgaccccaa tttcatagag ttgcctgatg acgtaacgca aatccagagg gtacaaaaac
     1921 agggcctgaa cggtcctgcc tgccaagagg caaagccctc ccgcgtaatc caaaaaatcc
     1981 caaacaacgg caccccagcg ttgcaacaaa ctccggaccg actcctggac attttcgttg
     2041 ggcggcaacg aaggcaagtt gtgtggagca gatgtcatag taactttggg cctttgatga
     2101 atgcaaaacc gtcaatcatg tttttgaaat cctgataata caaaagttcg cccggtaggg
     2161 tcacatacac aaaatcaaac acacacgcgt tcatcttgaa cacaaacgtg ctcatgaaaa
     2221 tctcgcggcc atccatgtcc ccctgagctt ccgtcaacaa ggccgcgcgt ccgtgtgtat
     2281 tatgcatttg agtttgttgg atctgaacat cagccaggcc acgcaggaga tcctgcgtca
     2341 gaccttccag tgaaccgtcg gcggcggcgc gctgacacca cgaatcgatg gtgatggtgg
     2401 aggcatcgtt tttgttcaag aaca
//
LOCUS       DATDUN010000866         5508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_248480, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000866 DATDUN010000000
VERSION     DATDUN010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248480"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VJC18_12000"
     CDS             <1..1189
                     /locus_tag="VJC18_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLD46147.1"
                     /translation="HYFGLNLGLRLRESETFLNLDVAHEVLWGGVYQVSIAPDADFSG
                     FVEAQGATVLQDFFQNENTIPLEARLGFTKGFLAEHNLKTTIFGGLGVTSGFNTPDAR
                     VGLKITYDHLVSRVETREVVKVVEPKMVRIEKELKELTIYYPTDGDKVDPFYDQKIAA
                     IAAILRDNPDLGPLYIVGHTDDVGSHGYNQRLSERRAGKSARSIMDNGLSDKQIVWVG
                     MGEIYPVVPNTSDANRALNRRTLFTFVKPQQLQEQYTKAGSLIGINTITGKQNDSYTE
                     VLKKLETQKQFVDDASGAQVIENYRDSSQVILEQDGTTTTKDSPAASPQVVPNQPRSK
                     TVVETKIETHDEALQRVETEALEEPQKTEKPKKEKKAKARDQKKSLIEEIDVIDEEDV
                     MDL"
     gene            1273..4158
                     /locus_tag="VJC18_12005"
     CDS             1273..4158
                     /locus_tag="VJC18_12005"
                     /inference="COORDINATES: protein motif:HMM:NF027513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HLD46148.1"
                     /translation="MKSKTTNHISYFAALTICLLLSSCQSAVLPHSGEQGQEGYQSIL
                     LDINTAKRDQERQLKNIVLPNGLEVLLISDTQATQSSAAMSVGVGSLQDPEEHQGLAH
                     FLEHMLFMGSQKYPRVDDFSEFVESHGGYSNAYTAPEETNYQLIVNHDAFEGALDRFA
                     QFFIAPIFDAHYVERERQAVNSEHQKNVQNEAWRDARLINQLARQNHPIRKFSTGNLD
                     TLRNATREVLLAFYEKHYSANLMKLVLKSNMSLSDMEKWVHEKFAEVPNHNLARTGFE
                     RDVFDFSQTPFLRIKPVTNRKSLSLIFEVPSLYEYLDSKPHYFLASLLGHEGQGSLLS
                     LLKREDLATGLSTDVDASSYSGRFEISINLSEKGEGQTPRIISHVYAYVDMLKKEGLK
                     KFYHDEIKTLGEVQYVFRTPSKGTGEVEAYASAMHLMKALEIDRKSSLYYQHVPKDFS
                     DLLALITPRNMLVVYVSPDVKTDQKELYYGAEYRLTAFDEAQRASWQSTTTETSFVFP
                     KPNPFIPQDLSIKTDDPQTVPYKVVDDERGVFWFMQDKALHVPRAKLAINLLTDRVNG
                     SPRDKLLSVLYREALYLSLVEWSYPMVEAGLGFSVDRADRGINLTVEGYSDKIPLVTG
                     QIIKRLKDITIDEKQFAGIKVNFRRLLDSWPFSPAHQQASYHASVILSQHDIHKDMYP
                     PVEEITLDEVKKFASGLYDQVAIEAVAYGNLTGRDLTTVVADLYADLQANILPQEKRI
                     EDEMVQLNPGEAFYYVPPTKSDNNSWLARVAFGPRSADLKAYLFLADAYLQTAFFTEM
                     RSQRQLGYLVQSGLAFHPKALGMSFVIQSGEYDPVWLEGQVLPWLKETARKIASMPDE
                     LFETYRLALIERLKEKYQTVDEWFGYVNSGALIEKGDFAFREKIIASLQRATKKELGT
                     IMQKAMDDDTRRMLSVYVLPPGKEISLPKKGVRITDIAAFKKDLPVY"
     gene            4241..4603
                     /locus_tag="VJC18_12010"
     CDS             4241..4603
                     /locus_tag="VJC18_12010"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLD46149.1"
                     /translation="MSKQFDYEKFDVFPVAVELMALIDETVHLMPRGGGEIADLLQQN
                     ARSIPLNLAAGTTEVTKERKLQCYYDARRSTIQCAAMVDVCNRLELVDQDHLFANRDL
                     LHRVLDMMTKLIEIEQEE"
     gene            4812..>5508
                     /locus_tag="VJC18_12015"
     CDS             4812..>5508
                     /locus_tag="VJC18_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HLD46150.1"
                     /translation="MQKNTLNFFMTVLLGMLICVMFARCDSNSSSGDPADCVGDECEE
                     ECVGAECDVNPIEDADGDTIADGDDNCPAVFNTQQTNSDTDALGDSCDLCPTDVDETN
                     ADTDADGTGDACDDDADNDGVLNTSDNCDTVANPEQTDADGDSYGKECDADDNNANIQ
                     VDDDKDGIDDTTDNCLASTNADQCDTDHDGKGNTCDTDDDDDGLLDDYDTCALDKNSQ
                     GYCYTDTDVDGVSD"
BASE COUNT         1445 a         1360 c         1477 g         1226 t
ORIGIN      
        1 gcactacttt ggtttgaatc tggggttgcg gctcagggaa tcagagacgt ttttaaacct
       61 cgatgtggcc cacgaagttt tgtggggagg ggtttatcag gtcagcattg cccccgatgc
      121 cgatttttcg ggctttgtcg aggcgcaggg cgctaccgtt ttgcaggatt ttttccagaa
      181 cgaaaacaca attcccctcg aggcgcgctt gggatttacc aaaggtttcc tggcagagca
      241 caacctcaaa acgacgattt ttggcgggct gggcgtgacc agcgggttca acacccccga
      301 tgccagagtg ggactcaaga tcacctacga tcacctggtg tcacgcgttg aaacgcgtga
      361 agtggtgaag gtcgttgaac ccaaaatggt tcggatcgaa aaagaactca aggaactcac
      421 gatttattac cccaccgatg gcgataaagt ggatccgttc tacgatcaaa agattgccgc
      481 gatcgccgcg atccttcgcg acaatcccga tctgggaccg ctctacatcg ttgggcatac
      541 ggacgatgtt ggctcgcatg gctataacca gaggctttcc gagcgtcgcg cgggtaaatc
      601 agcgcggtcg atcatggata acgggttgag cgacaaacag attgtctggg tggggatggg
      661 cgagatttat cccgtggtgc ccaacacctc cgatgccaac cgcgcgctca accggcgtac
      721 cttgtttacg ttcgtcaaac cacagcagtt gcaggaacaa tacaccaagg cgggcagttt
      781 gatcgggatc aacacgatca cgggcaaaca aaacgattcc tacacagagg tgctcaaaaa
      841 actggaaacg caaaaacagt tcgttgatga cgccagcggt gcccaggtga tcgagaacta
      901 ccgggattcc tcgcaggtca tcctcgaaca ggatggcacc accaccacca aagactcacc
      961 agccgcctct ccgcaggtgg tgcccaatca accgcgtagc aaaacagtgg tggagaccaa
     1021 gatcgaaacc catgatgagg ccctgcaacg cgtcgaaaca gaggctttag aagaacctca
     1081 aaaaacagag aaacccaaaa aagaaaaaaa agccaaggcg cgtgatcaga aaaaaagtct
     1141 catcgaggag atcgatgtca tcgatgaaga agacgtgatg gatttgtgaa caaggcgcaa
     1201 cgctcaaagc tcacggccaa cggtctacgg ctgaccttca agggcacagg cggttcacga
     1261 tccgagaaac atatgaaatc gaaaacaacc aatcacatca gttattttgc cgcgctgaca
     1321 atttgtttat tgctttcctc ttgccagtca gcggtcctgc cgcatagtgg tgagcagggc
     1381 caagagggtt atcaatcgat cctgctcgac atcaatacag ccaagcgcga tcaggaacgc
     1441 caactcaaga atattgtttt acccaacggg cttgaggtgt tgctcatctc tgacacgcag
     1501 gcaacccagt cctcggccgc tatgagcgtg ggggtgggga gtttgcagga tcccgaagag
     1561 catcaggggc tggcccattt tctggagcac atgcttttca tggggtcaca aaaatacccc
     1621 cgcgtggatg atttttccga attcgtggaa tcgcatgggg gttacagcaa tgcctacacg
     1681 gcccccgagg aaaccaatta ccaacttatt gtcaatcacg atgcctttga gggggccctg
     1741 gatcggtttg cccagttttt catcgcaccg atcttcgatg cgcattatgt ggagcgcgag
     1801 aggcaggcag tcaattcgga acaccagaaa aacgtacaga acgaggcttg gcgcgacgca
     1861 cgattgatca atcaactggc cagacagaat caccccatac gtaaattcag taccggtaat
     1921 ttggacacac tgaggaacgc cacgcgcgag gtcctgctcg cgttttacga aaaacattat
     1981 tcagcgaacc tcatgaagct cgtgctcaaa tcgaacatga gtctgagtga tatggaaaaa
     2041 tgggtgcacg aaaaattcgc tgaggtacca aaccacaatc tggcacgaac aggttttgag
     2101 cgcgatgttt ttgatttcag ccagaccccg tttttgcgca tcaaacccgt gaccaaccgc
     2161 aagagcttga gcctgatttt tgaggtccct tccctctacg aataccttga ttccaaaccc
     2221 cattattttt tggcttccct gctggggcat gaaggacagg gttctctgct ctccctgttg
     2281 aaaagagagg atttggcaac aggcttgtcc acagacgtcg atgcgagttc ttattccgga
     2341 cggtttgaga tcagcatcaa cttgagcgaa aagggcgagg ggcagacccc gcgcatcatc
     2401 agtcatgtgt acgcctacgt ggacatgctc aaaaaagagg gacttaaaaa gttttatcac
     2461 gacgagatca aaaccctggg tgaggttcag tacgtgtttc gaacgccttc gaagggaacg
     2521 ggcgaggtgg aggcgtatgc gtcagcgatg cacctgatga aagcactcga gatcgatcgc
     2581 aaaagctccc tgtattacca gcatgtccca aaggattttt ccgatcttct cgccctcatc
     2641 acgccacgga atatgcttgt ggtttatgtg tcccccgatg tgaaaacaga ccagaaggaa
     2701 ttgtattatg gggccgagta cagacttacg gcgtttgacg aggcgcagag ggcatcgtgg
     2761 cagagcacta ctaccgaaac cagttttgtg tttcccaaac cgaatccgtt tatcccgcaa
     2821 gacttgtcga tcaaaacaga tgatccgcag acggttccgt acaaggttgt tgacgacgaa
     2881 cgaggcgtgt tctggttcat gcaggacaaa gccttgcatg tgccccgggc caagctggcc
     2941 atcaacctcc tgacggatcg cgtcaacgga tcgccgcgcg ataaattgtt gtcggtcctg
     3001 tatagagagg ccttgtattt gagtctcgtg gagtggtctt atccgatggt cgaggcgggt
     3061 ttgggctttt cggtcgatcg cgccgatcgg gggatcaacc ttacggtaga gggttactcc
     3121 gacaaaatcc cgcttgtgac agggcagatc atcaaacgat taaaagacat cacgatcgac
     3181 gaaaaacaat tcgcgggcat caaggtcaac tttcgtcggc ttttggacag ctggccattt
     3241 tcaccagccc atcagcaggc cagttatcat gcctccgtga ttttgagtca acacgacatc
     3301 cacaaggata tgtacccgcc ggtcgaagag atcactttgg acgaggtcaa aaaatttgcc
     3361 agtggtctgt acgatcaagt agccattgaa gctgtggcct acggcaatct aacgggacgc
     3421 gacctcacga ctgttgtggc cgatctgtat gcggacctgc aggccaacat tcttccccag
     3481 gaaaaacgca tcgaagacga gatggtgcaa ctcaatcccg gggaggcctt ttactatgtg
     3541 cccccgacca aaagcgacaa caatagctgg ttggcgcgtg tggcctttgg tccgcgcagt
     3601 gcggatttaa aggcgtacct gtttttggcc gatgcgtatt tgcagaccgc tttttttacc
     3661 gagatgcgct cacaaaggca gttgggttat ctcgtgcaaa gcggtctggc ctttcacccc
     3721 aaggccctgg gcatgagctt tgtgattcag tcgggagagt atgatcctgt ttggctggaa
     3781 gggcaggtcc tgccctggct taaagagaca gccaggaaaa tagcctctat gcccgacgag
     3841 ctgtttgaga cgtaccgtct ggctctgatt gagaggctca aggaaaaata tcagaccgtt
     3901 gatgaatggt tcggttacgt gaattccggg gcactgatcg agaaggggga tttcgccttc
     3961 agggaaaaga tcattgcgtc cttacaaagg gcaaccaaaa aggaactggg tacgatcatg
     4021 cagaaggcga tggatgacga cacgcgcagg atgctgagtg tctatgtgtt gccaccgggc
     4081 aaggaaattt ccttgccaaa aaaaggggtg cgcattactg atattgcggc gttcaaaaag
     4141 gatctgccgg tttactagct tgtcagtcct gcgcaggcag gaatccagcg caaccttaat
     4201 ggaaatggat cccagctttc gctgggatga caaaaaattt atgagcaaac aatttgatta
     4261 cgaaaaattc gatgtgtttc ccgtagcggt ggagctgatg gccctcattg acgaaaccgt
     4321 gcacctcatg ccgcgtgggg gaggggagat tgccgatctc ttgcaacaga acgcgcgatc
     4381 gattcccctt aatctcgccg ccgggacaac cgaagtgacc aaagaacgca agttgcagtg
     4441 ctattacgac gccaggcgat caacgattca atgcgccgcc atggttgatg tgtgcaaccg
     4501 tctggaactg gtcgatcaag accacctctt cgccaaccgc gatctcctgc accgcgtgct
     4561 cgacatgatg accaagctca tcgagatcga acaagaggag taaaactccc tcacgactaa
     4621 gtccctcaag gttccctcac tttctctttc aatttgataa ttctgtggtg tcttctctgt
     4681 aagtctggcg tcacgccagc ccccataaaa aaatcataaa caaaaacaat gtgatacaaa
     4741 ctgtcatcta agggattaat ggcatgggtt ttgctacata attcgtgagg tcgtaaaaaa
     4801 aaggatttaa tatgcaaaaa aacaccctga atttctttat gacagtttta ttgggcatgc
     4861 tcatttgtgt gatgtttgcc cgttgtgatt caaattcctc ctcaggcgat cctgcggatt
     4921 gcgttggcga tgaatgcgaa gaggaatgcg tgggggcgga gtgcgatgtg aaccccattg
     4981 aggatgccga tggcgatacg attgccgatg gcgatgacaa ctgccccgcc gtgtttaaca
     5041 cacagcagac caacagcgac accgatgctt tgggagattc ttgcgatttg tgtcccacgg
     5101 atgtggacga aacgaacgcg gatacggacg cagatgggac aggcgatgcg tgtgatgatg
     5161 acgcagacaa cgatggggtg ctgaacacca gcgacaactg cgacaccgtg gccaatcccg
     5221 agcaaaccga tgctgacggc gatagttatg gcaaagagtg tgatgccgac gataataatg
     5281 cgaacattca ggtggatgac gacaaagatg gcatcgacga taccacagac aattgtttgg
     5341 ccagcaccaa tgccgatcaa tgtgataccg atcatgatgg caagggcaat acgtgcgata
     5401 cggacgatga tgatgatggc ttactggatg attacgatac ttgtgctctg gacaaaaact
     5461 cgcagggata ttgctacaca gacaccgatg tggatggcgt gagtgatg
//
LOCUS       DATDUN010000867         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_539009, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000867 DATDUN010000000
VERSION     DATDUN010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539009"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VJC18_12020"
     CDS             <1..336
                     /locus_tag="VJC18_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HLD46151.1"
                     /translation="NLSVITGKTVGVNCFYEEQARLDGALYPGYSQEEKMAFIHRIYK
                     MGVRNFEMEIPQFASFCMRAGLRGAGVCVALLDRLKGDQVTPTPEELAEFSDRAQQVV
                     VNYICAKES"
     gene            complement(487..1152)
                     /gene="phoU"
                     /locus_tag="VJC18_12025"
     CDS             complement(487..1152)
                     /gene="phoU"
                     /locus_tag="VJC18_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HLD46152.1"
                     /translation="MGQHIDHYFDEALASLKQQILLMGSKVEAMLADCILSLKQRDLG
                     LAATVVESDHSVNALEVSIDEQCIQLLARYQPAAGDLRFITRGLKIVTDLERIGDLAV
                     NMAQRITDILTQGTMPIDLTGMAGTVQQLLKQSLDAFVQGDVTMAEKVLQSDDMVDDL
                     TAQFVIQLIEIAEKEPKNFKACFSTASIVKYLERISDHSTNIAELTIFMVKGRDVRHG
                     KPQ"
     gene            complement(1168..1914)
                     /gene="pstB"
                     /locus_tag="VJC18_12030"
     CDS             complement(1168..1914)
                     /gene="pstB"
                     /locus_tag="VJC18_12030"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011171042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HLD46153.1"
                     /translation="MTILTCHDLNLFYGRFQALKAVNLSVKRNTITALIGPSGCGKST
                     LLRVFNRMNDLISGVRVSGDVTAFEKSIFQMNVMELRKRVGMVFQRPNPFPLSVFDNV
                     AYGPRIHGSERSADLNNIVQDCLSAVGLWNDLKDKLKTNALQLSGEQQQRLCIARLLA
                     VKPDILLMDEPCSALDPIATARIEDLMKELKKNYTIIIVTHNMQQAARVSDVCGFMLL
                     GDLVELGETTQIFTAPNEKRTEDYISGRFG"
     gene            complement(1917..2669)
                     /locus_tag="VJC18_12035"
     CDS             complement(1917..2669)
                     /locus_tag="VJC18_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HLD46154.1"
                     /translation="MNVKLSTKDLHVSFSSHRVLRGVSFDIFEHEILSIIGPANSGKS
                     TLLSTFNRMIDRYHDAKMTGTVLLDGMNVATMEDVASLRCRVGIVYATPIPLPMTIRE
                     NILFGPSLTKRLSRVEASDLVEKTLKASVLWDEVKDRLDSSALALSGGQQQRLCMART
                     LAVNPEVILFDEPCSGLDPISTAQVEEAMKELKKDHTLVLVTNNTKQAARVGDRTAFL
                     LMGELVEVDQTNIIFTKPTDKRTEDYVTGKFG"
     gene            complement(2670..>2938)
                     /locus_tag="VJC18_12040"
     CDS             complement(2670..>2938)
                     /locus_tag="VJC18_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HLD46155.1"
                     /translation="ILTGVILGIGRSIEETAVVIFTAGSAIQMPQSIFDSARTMAVHF
                     YILAREGVSNENAYATAAVLICTILIINISAYTLMHRLMKGRRA"
BASE COUNT          756 a          692 c          712 g          778 t
ORIGIN      
        1 aatttgtcgg tgatcactgg caaaacggtt ggcgtgaatt gcttttacga agagcaggcg
       61 cgtctggatg gcgcgttgta tccgggttat tcgcaggaag agaagatggc gttcattcac
      121 cgtatctaca aaatgggcgt gaggaatttc gaaatggaaa tcccccagtt tgcctcgttt
      181 tgcatgcggg cggggcttcg cggggcaggt gtgtgtgtgg ctctgctcga tcgtctcaag
      241 ggtgatcagg tgacccccac gccagaggaa ttggccgaat tttccgatcg cgcccagcag
      301 gtggtggtga attatatttg tgcgaaggaa tcgtgattgt gtgaattcca aaacctctgc
      361 gtcggtcaaa tccttgattc taccggtggc ctttttattt gtcatcaaat catcgatggc
      421 caaaatgcag acagggatgc ctacgatcga aaattttctc agtcttattg atgcttggga
      481 aatttcttat tgtggctttc cgtgcctcac atcgcgcccc ttcaccatga aaatggtgag
      541 ttcggcaatg tttgtggagt ggtccgaaat acgctccagg tatttgacaa tagaggctgt
      601 tgagaaacag gctttgaaat tttttggttc tttttcggca atctcaatca attgaatcac
      661 aaactgtgca gttaaatcat ccaccatgtc atcactttgt aaaactttct cggccattgt
      721 cacgtcaccc tgaacaaagg catccaggct ttgtttcaac agctgctgta ccgtgccagc
      781 catgcctgtg agatcgatgg gcattgtccc ttgcgtgaga atatcggtaa tgcgctgggc
      841 catgttgaca gctaaatcgc caatgcgttc caagtccgtt acgattttga ggccccgcgt
      901 gataaaacga agatctccag cggcgggttg ataccgggcc aaaagctgaa tgcactgttc
      961 atcaattgac acttcgaggg cgttcacaga atgatcagac tcaacaaccg ttgcggccag
     1021 cccaaggtcc ctctgtttga gagacaggat acaatcagcc agcatggctt caaccttgct
     1081 tcccatgaga aggatctgtt gttttaaact ggccaaagcc tcatcaaaat aatgatcaat
     1141 gtgctgaccc ataaatatct cctcgattta tccaaatcgt cccgaaatgt aatcctcagt
     1201 ccttttttca ttgggtgccg taaaaatctg cgtggtctct ccaagctcaa ctagatcgcc
     1261 gagtagcata aacccacaaa catccgatac ccttgccgct tgttgcatgt tgtgagtgac
     1321 aatgataatg gtgtagtttt ttttgagttc tttcatcaag tcctcaatgc gcgccgtggc
     1381 tattgggtcc aatgcagagc aaggttcgtc catcaaaaga atatcgggct taacggccaa
     1441 cagccttgcg atgcacaaac gctgttgttg ttcgcctgaa agttgcaggg cgtttgtttt
     1501 gagtttgtct ttcaaatcgt tccacaaacc cacggccgaa agacagtcct gaacaatgtt
     1561 gttcaggtcg gcagatcgct cgctaccgtg aatgcgcggc ccataagcca cattatcaaa
     1621 aacagagagg ggaaaaggat ttgggcgctg aaaaaccatc cccactcttt tgcgcaattc
     1681 catcacgttc atttgaaaaa tagatttctc aaaggccgtg acatcgcccg aaacccggac
     1741 gccggaaatc aggtcattca tgcgattaaa aacacgaagc agagttgatt ttccgcaacc
     1801 tgagggacca atcaaggccg taattgtgtt tcttttgaca gatagattga cagccttgag
     1861 cgcttgaaaa cgtccgtaga ataaattgag gtcgtggcat gtgagaatag tcattgttat
     1921 ccaaatttcc ctgtcacgta gtcttctgtt cttttgtctg ttggttttgt gaaaataata
     1981 tttgtttgat caacctccac cagttcaccc atgagcaaaa atgctgtgcg atcgccaacg
     2041 cgcgcggctt gttttgtgtt gttggtgacc aagacgagcg tgtgatcttt tttgagttcc
     2101 ttcatagcct cttcaacctg tgcggttgaa ataggatcca aacctgaaca aggttcatca
     2161 aacagaatca cctcgggatt cacagccaac gtgcgcgcca tacagagtcg ctgttgttgg
     2221 ccgccggaca gggcgagcgc agatgaatcc agtcgatcct tcacttcatc ccaaagcacc
     2281 gaagctttga gtgttttttc aaccagatca cttgcctcaa cacgagaaag acgcttggtg
     2341 agtgaagggc caaaaagaat attttcacga atcgtcatgg gaagtggaat gggcgtggcg
     2401 taaacaatgc ccacgcgaca gcgcaaagag gcgacgtcct ccatcgtagc aacgttcatt
     2461 ccgtccaaca agaccgttcc tgtcattttg gcatcgtggt atctgtcaat cattcgattg
     2521 aacgtggaga ggagtgttga tttgcccgaa ttcgcagggc ctatgatgga gagaatttca
     2581 tgttcaaaga tatcgaacga aacacctctc agaacgcgat gggatgaaaa ggagacatgg
     2641 agatctttgg tgctcagttt gacattcacc taggctcgcc gtcctttcat caatcgatgc
     2701 atcagtgtgt acgcagaaat gttgatgatc agaatagtac aaattagcac agccgctgtg
     2761 gcgtaggcgt tttcgttgga gacaccctca cgggccaaaa tataaaaatg aaccgccatg
     2821 gtacgagcag aatcaaaaat cgactgcggc atctggatgg ctgagcccgc tgtgaaaata
     2881 acaacggccg tttcttcgat cgatcgacca attcccagga tcacacccgt caggattc
//
LOCUS       DATDUN010000868         1584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_414594, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000868 DATDUN010000000
VERSION     DATDUN010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414594"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /gene="amrS"
                     /locus_tag="VJC18_12045"
     CDS             complement(<1..916)
                     /gene="amrS"
                     /locus_tag="VJC18_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HLD46156.1"
                     /translation="MSKSTTIQFWHPVGLGRIQCDICPRHCRLREGQRGFCFVRACEG
                     SKIVLTTYGWTSGFAVDPIEKKPLHHFLPGTSVLSFGTAGCNLSCVYCQNWHMSRSRS
                     KNAMSVQATPQEVACLALQQGCPSVAFTYNEPIIFFEYALAVSVECHARGIKTVAVTA
                     GMINEKPRKIFFEHIDAANVDLKGFSQAFYKKNCSGNFQTVLDTLVYLKKETGVWLEI
                     TNLLIPGENDADDEIDRMTKWIATELGPEVPLHFSAFHPAHKMLYKPRTPVATLLRAR
                     EIALRNGLYYVYTGNVPDTKSQSTDCHRC"
     gene            1042..>1584
                     /locus_tag="VJC18_12050"
     CDS             1042..>1584
                     /locus_tag="VJC18_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLD46157.1"
                     /translation="MNSADYVFRINMKDIALTRNIGISAHIDSGKTTLSERILFYTGK
                     IHKISEVRGKDGVGAKMDSMELEREKGITIQSAATHVDWKDHGVNLIDTPGHVDFTVE
                     VERALRVLDGAVLVLCGVAGVQSQSMTVTRQMNRYKVPRIAFVNKLDRTGANPFRVVT
                     QLREKLDLNAHLINIPIGLED"
BASE COUNT          421 a          396 c          375 g          392 t
ORIGIN      
        1 gacaccggtg acagtcagtg ctctggcttt tggtgtccgg cacattcccc gtatacacat
       61 aatacaatcc attgcgcaga gcgatctctc tggcccggag caacgtggcg actggggttc
      121 tgggcttata caacatcttg tgggcagggt gaaaggcgct aaagtgcaaa ggcacctcag
      181 gccccaactc cgtggcgatc catttggtca ttcgatcgat ttcatcgtct gcgtcatttt
      241 cacccgggat gagaagattg gtgatctcca gccagacacc tgtttccttt ttgagataca
      301 ccagcgtgtc cagcacggtc tgaaaatttc cagaacaatt ttttttataa aaggcctgtg
      361 agaacccctt caaatcaaca ttggccgcgt cgatgtgttc aaaaaatatc tttctgggtt
      421 tttcgttgat catgccagca gtcactgcca cagttttgat cccacgcgca tgacattcca
      481 cggacacggc cagggcatat tcaaaaaaaa taatcggctc attgtaggta aaggcaacac
      541 tgggacagcc ttgttgcagg gccaggcagg caacctcctg aggagtcgcc tgcaccgaca
      601 ttgcgttctt cgaacgcgat ctactcatgt gccaattctg gcaataaacg cacgacaaat
      661 tacaacccgc tgttccaaag gataaaaccg atgtgcccgg caggaaatgg tgtaagggtt
      721 ttttttcgat gggatccacc gcaaacccac tggtccagcc atacgtggtc agaacaattt
      781 tggaaccttc acaggcccgt acaaaacaga acccacgttg tccctcgcgc aaccggcaat
      841 gacgtggaca tatgtcacat tgaatccttc ctaatcccac agggtgccag aactgtatag
      901 tggtagactt gctcatgcta tcatgatgat attataacat tatttatgtg tcgtataaaa
      961 cccctttatt ttattaataa tgttgtgtta gtaggggcgc ccttttgata atatagaggc
     1021 tcatatacga agtaaattga aataaattcg gctgactacg tatttaggat aaacatgaaa
     1081 gacattgcac tcacaagaaa cattggtatt tcggctcaca ttgattcggg taaaaccacc
     1141 ttgagcgaaa gaattttatt ttacactggc aaaatccaca aaatatcgga ggtgcgtggc
     1201 aaagatggcg ttggcgcaaa aatggattca atggagttgg aacgagaaaa gggcatcacg
     1261 attcaatccg cggccactca cgtggactgg aaggatcacg gagtcaacct cattgacaca
     1321 cccgggcacg tggactttac cgttgaggtt gaacgggcct tgcgcgtgct tgatggcgct
     1381 gtcttggtgc tctgcggcgt cgcgggcgtg cagtctcaat ccatgacggt gacccgtcaa
     1441 atgaaccgtt ataaagtgcc gcgcattgcc ttcgtcaaca aactggatcg tacgggcgcc
     1501 aatcctttca gggtcgtcac ccaactgcgg gaaaaactcg acctgaacgc tcacctcatc
     1561 aacatcccca tcggcctcga agat
//
LOCUS       DATDUN010000869         3463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_455804, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000869 DATDUN010000000
VERSION     DATDUN010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455804"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VJC18_12055"
     CDS             complement(<1..721)
                     /locus_tag="VJC18_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46158.1"
                     /translation="MNSTALSIQHSYSIYAGDFHLHTLGDSGPFQNPAFAAGYEHSAL
                     RSIVAQSVSPAYLERLLLGASRRVQSHIRTNTDNGDVAARFTGIVGLGDYTDMACSQE
                     RDMLLTSAAHLAGIDPNITFTMMVPGNHDGGQYMGSLWSKDNLFGLPFVGGNDLRQGA
                     CGCGRALSVEERIETLRQITGFGTDAESVTAVSRMLGNGFDTPHGRTYSFDHPREVFD
                     LVWKQLPGEERWEAVIHYGSNA"
     gene            complement(864..1076)
                     /locus_tag="VJC18_12060"
     CDS             complement(864..1076)
                     /locus_tag="VJC18_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46159.1"
                     /translation="MNKKSMLRMINLLLFFNFILVATSGLWHELISHNLYEKLHVIPG
                     FSLIALVVAHLVLNAGWIKQIFSPPS"
     gene            complement(1104..3209)
                     /locus_tag="VJC18_12065"
     CDS             complement(1104..3209)
                     /locus_tag="VJC18_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46160.1"
                     /translation="MKEYLKNIPTKYPVYFKLVVFQLTAVLLGSAVLFILYHHISGFF
                     FDRAFDENKAAMHRKIDKVQEQWRTWKYMSLNDALNPALEEFKDAFQLESIAVIDIVD
                     LPKKWDRYDIIIPEATDPVINYVVYARLSPLNVGYLYKMHRLFLSVLLASFVLTLAVI
                     WFASLMLFRWYYLPIGQLNEAVANLNAGDRLKIDHIRAYDALGSFISHLKQMTHYIRG
                     YERMSSIGKMSSQIVCVLKPYFNDLKGLTEIEGAWSGADRQKMNHAVRGIENVLNDLN
                     EKSRRVNESLGSLQDESLNINGASQEEFTAQLLFSVLDTLVSEKRMQFHSLSHLRLDL
                     VSAKHAYGLFVNIKTLVFRKLVSNIIDLFAEQAGRKENIVLKIEGDYHHARLTISDDV
                     MADFENEYLDKRSPEQSFLQKFDPSSHLDKARLVFQAWGGDLAITIIENRATLVSVDL
                     PRQPHQNWLLSHLELERYSHVVILDDDKAIHLHWENILIQAEIKPSHIIHFYKPEEFI
                     QWFVSERKHQELRSGYDTLLTAYEFVGSDKTGIDVIEEFNLKGRSILVTSQYKRQRVL
                     KLCEGMDLKILPKALVGTVPIIVSDIVEKPDCILIAQDEHVQVYWRMEARLSGKTILT
                     YNGPEDFYRYIHNYDRSVPIYITTILSGEVRGIDFSRELDKLGFKNIYITTGRDKTQF
                     PAMPWIRGIVTKEPPWKYH"
     gene            complement(3211..>3463)
                     /locus_tag="VJC18_12070"
     CDS             complement(3211..>3463)
                     /locus_tag="VJC18_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46161.1"
                     /translation="DPRSTIHDPRSAIRDPRFGQIQKNQTDRLLSKIPYSNIDFFAQI
                     NHSPKGLFSGYVLRGTSGKTVLSLRVVLISGWRYSEVSC"
BASE COUNT          943 a          887 c          779 g          854 t
ORIGIN      
        1 aagcattgct gccataatga atcaccgcct cccaacgctc ttctccgggt agttgcttcc
       61 acaccaaatc aaaaacctct cggggatgat caaaagaata ggtccttcca tggggtgtat
      121 caaaaccatt gccaagcatc ctactcacag cagtgaccga ttcagcatcc gtgccaaacc
      181 ccgtgatctg ccgtagtgtt tctatacgct cctccacaga aagagcgcga ccacaaccac
      241 aggccccttg ccgcaagtcg tttcccccca caaaaggcag accaaagaga ttgtccttgg
      301 accagaggct ccccatatac tggccaccat cgtgatttcc ggggaccatc atcgtgaatg
      361 taatattggg atcgatgccc gccaagtggg cggccgaggt gaggagcatg tctctttcct
      421 gagaacacgc catgtctgta tagtccccca aaccgacaat accggtaaat cgcgcagcca
      481 catccccgtt atctgtattg gttctgatat gactttggac acggcgactc gcccccaaaa
      541 gcaatcgttc cagataagca gggctaacgc tctgagccac tatggatcgc agggcagaat
      601 gctcataacc cgccgcgaat gccgggttct gaaaaggacc actatcaccc agcgtatgca
      661 gatgaaaatc ccccgcgtag atcgaataac tgtgttgaat tgacaaggcc gtggaattca
      721 tgcctttttt ctaacctccc ccgtttgacc cgctattatt gggcgcaaca cgcttggggt
      781 tgcttactgg caatattttt tactgataac agtattataa ggagtgcgct gaatgattgt
      841 cattatgaag attgaataat cttctatgaa gggggcgaaa aaatctgttt gatccagcct
      901 gcgtttaata ccagatgagc gacaaccaag gcaatgagtg aaaagccggg gatcacgtga
      961 agtttttcgt aaagattgtg actgatcagc tcgtgccaca gtcccgaagt ggccaccagg
     1021 ataaaattaa aaaaaagtaa aagattaatc atcctgagca tgcttttttt attcacaaaa
     1081 ctctcctctt ttgtggaggt ttcctaatga tacttccagg gtggctcttt ggtgacgatc
     1141 cctctgatcc acggcattgc cggaaattgg gttttatcac ggcccgtggt gatgtaaata
     1201 ttcttgaaac ccaatttatc cagttccctg ctgaaatcaa tgccacgaac ctccccgctg
     1261 agaatcgttg tgatgtagat gggaaccgac ctgtcataat tgtgaatgta gcggtaaaaa
     1321 tcttccggac cattgtaggt caggatcgtc ttgccgctca agcgggcctc catgcgccaa
     1381 tacacctgaa catgctcgtc ctgcgcgatc aggatacagt ccggcttttc cacaatgtcc
     1441 gatacaatga tgggcacagt ccccaccagc gccttgggca atatcttcaa gtccatgccc
     1501 tcacacagct tgagcaccct ttgcctcttg tactggctgg tcaccaaaat ggatcggccc
     1561 ttcaaattga attcctcaat cacatcgatc cccgttttgt cggagcccac aaattcatag
     1621 gccgttaaca acgtgtcgta ccccgacctg agttcctgat gttttcgttc actcacaaac
     1681 cactggataa actcttcggg tttgtaaaaa tgaatgatgt gcgatggttt gatctcggcc
     1741 tggatcagga tattctccca atgcaggtga atggctttgt cgtcatccag gatcacgaca
     1801 tgagagtagc gctccaactc aagatgcgac aacaaccaat tctgatgcgg ctgtctgggc
     1861 aaatcgacag acaccagagt cgcgcgattt tcgataatcg tgatcgccaa atcgccaccc
     1921 caggcttgaa acacaagacg ggccttgtcc agatgggatg agggatcaaa cttctgcaga
     1981 aaagactgtt caggcgagcg cttgtccaaa tactcattct cgaaatcggc catcacatcg
     2041 tcactgatgg tcaaccgggc atgatggtaa tccccttcga ttttgagaac aatgttttct
     2101 tttcggccag cttgctcagc aaaaagatcg atgatgttcg acacaagctt gcgaaaaacc
     2161 agggtcttga tgttcacaaa caatccataa gcatgtttgg cagacaccaa atccaatctg
     2221 aggtgcgaca gcgaatgaaa ctgcatgcgt ttttcggaca ccaacgtatc caaaacactg
     2281 aacaacaact gggctgtaaa ctcctcttgc gacgccccgt taatgttgag actttcgtcc
     2341 tgaagactcc ccagtgattc attcacacgc cgactttttt cattgaggtc attcagaaca
     2401 ttttcgatgc cacgaaccgc atgattcatt ttctgccgat cagccccgga ccaagcacct
     2461 tcgatctccg taagcccctt gaggtcattg aaatacggtt tgagcacaca aactatctgg
     2521 gacgacatct tgccaatgga agacatgcgc tcgtaaccac gaatgtaatg ggtcatctgt
     2581 ttgaggtgac tgatgaaaga ccccagggca tcataggcgc gaatgtgatc gatcttcaaa
     2641 cgatcgcctg cattgaggtt cgccacagcc tcattcagct ggccaatggg gaggtaatac
     2701 catcgaaaca acatcagtga cgcaaaccag atcacagcca aagtgaggac aaatgacgcc
     2761 aacagcacag acaagaacag tcgatgcatt ttatagaggt accccacatt caggggagaa
     2821 agcctcgcat acaccacata gttgatcaca gggtcagtgg cttcggggat gatgatgtca
     2881 taccgatccc attttttggg caggtctaca atatcgataa cagcgatgga ctccaattgg
     2941 aaggcgtctt tgaattcttc caatgccggg ttcagcgcgt cattgagaga catgtacttc
     3001 cacgtacgcc attgttcctg gaccttgtcg attttgcggt gcatcgcggc tttgttttca
     3061 tcgaaggctc gatcgaaaaa aaagcccgaa atgtgatgat acaaaataaa caggaccgcc
     3121 gaccccaaca aaacggcagt gagttgaaag acaaccagtt tgaaatagac agggtacttg
     3181 gttgggatat ttttaagata ttctttcatg ttaacaggac acctctgaat aacgccaacc
     3241 agaaatcaaa accacacgca agctcaatac agttttgccc gatgtgcccc tgagtacata
     3301 ccccgaaaac aggcctttcg gagaatgatt gatttgagca aaaaaatcaa tgtttgaata
     3361 cggtatttta ctcaacaacc tatctgtctg atttttttgt atttgtccaa atcgtggatc
     3421 gcggatcgcg gatcgtggat cgtggatcgt ggatcgtgga tcg
//
LOCUS       DATDUN010000870         5820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_248578, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000870 DATDUN010000000
VERSION     DATDUN010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248578"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VJC18_12075"
     CDS             <1..1166
                     /locus_tag="VJC18_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HLD46162.1"
                     /translation="LTHRGMNNLAVRVAELAKEEGLQLNLVKVDDGQEQRDERLSALA
                     GDKIKIQFYRTGETLAKHVTEYDVVVASPWVMSRPEFSHQFSHLFIDEAGQLSLANAL
                     AMTHCAANLILLGDQRQNRQPLQARHPGLSGLSVLDYMLSGLGTDVPVVPDDFGLFLG
                     QSFRMAPPIAEFASQEFYDGRLQTAEVATRRQLIAPERPVPVEVQGKRFMFPPAGIVA
                     LPITGPIHSRQNPEEALAVQAILESLKHFHVEQRNGAGDVVESAPLDSARVFVMAPFT
                     AQVNALARELPPDVLVSTVDGLQGRENSVVLYSLGVSDPDSQHGVTFAMDPRRINTAL
                     TRAQVLFILLANPHLLDRRPGTIAEMKNLNRLAMLLRRYAPEPASGPDRDSAR"
     gene            complement(1155..5783)
                     /locus_tag="VJC18_12080"
     CDS             complement(1155..5783)
                     /locus_tag="VJC18_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46163.1"
                     /translation="MIRKDKNPQAGIDVLTRLRKGSGDCLSQTHTKLTTRISVLEGAV
                     AALVRLQGGNTEHRLDDGARSQIVLGNVKSVLFKYGALEAVFRARIERLLEVTGLADE
                     MYRKIAASTQMDFERGVKSFADVYALRLAEMKSWLPYSHSKWEEWFILVKLQLAQNLH
                     EEFERAKNAILGPDFVPQNTTQLEAYLSLEAREVDIENGDLIDFLKRRDENKESLQEL
                     ESASATCGNTSFHQHMIKELKGKIAGCEAGIRLAKLYFEQGPALDAFIDQGYRKPPVG
                     CLESKHGLFVDSLESGGLRLRIAHDMLIQAPKRAKKIRKDHHLNFLLSEKDSVRLAGL
                     IKTYGYRKASPAVLEQILNKISDHLATSFEECLQHRDGGNRELQYCEIFHFIRDCLPT
                     LISYFSKCLPTQTRTLIQNVIKKTVEIHCSSAVKIHGYYHDNPHFLKNLAELIRGAKY
                     CAEIDLKECETSLLDKATAELIENLNSLNKESWLNALHGFQEQLDEMGMLYSIHYSQY
                     ANRAQGVDRLVWLIFKAAGDAGVSREQIKHLVYLMIPAMMPDAAKQGLNREAMFSDMI
                     QLANNEKFWGVFKGQLDIRQTAHVYTNEPALWTQEETELNSIVEMLVANNEESSIPAQ
                     VRQRLRQIEEDKLNAIYDIFMAIPIDHWTLAEKTHAQMQRKATKYDPQPGQLTELALK
                     RLSLERPWDLARAAFFLDPDVQADCRDAIAGFYKKSFQWFLMRHGSVTKASYEFWKAF
                     DRAHLETTYSSLRCIEAGVSSSFSFVSAGKRTISDLSVYAEYVAGVEELINEKSGKYS
                     SKGSASDELLLRCFARTLDDFFSDLRCVTLYPRALVARLLLFRVKNKMGLLPDKIQDV
                     LGYLWQFRDFEQEVGELFLYPGLIRLCRYEGFKTDIAKWQIEHLPGFRQHQQDLKAYP
                     AQLPTPQEVRKIVRDVRALLQEQFAEPTPASYSVLDFVEAALLPGENETRLLAELRLN
                     ENNWVQMRALYALDAPDFLAQGITTKQERLDLICYLIGVSPHVPHLQKYQNKQDQEDS
                     QDQIIRFKALYQQNGALFQAMTLQVILGDELKTLQGTFVYDAVKNIIMDGAYEDPALS
                     EIFDTYMNVLPAGERKILIALALANYDPTTGHSSFRRLLETSGPLGAKAAQNLVTSGL
                     VTGTLRDDLMSSFDFALPPSREIIIRQLKQIFGDNYIKILAVGKLAGSGSLNYAVEVY
                     VRLSTGEVRRVCVRIQKEHVSGLIENEHKVWLKVIEQLKAPDRPPQVLRYGMLIENLM
                     QQAYDTLKPGGVELDLEQERSKAALGLRAYQHAEISPQTGLRVTAVAPDLEIQTMIQP
                     EYRKQASVYAFVDHTKLDKIRPRTLRVAIAAQIVDAEIKALDEGRLVFDPDGHQGNWP
                     VDLANGNLVRVDYAQITTITKEELDLFKFIFRALINPSRNAGTELKKLLRSRFTDIFE
                     GEGKPQNLDEALVYVLSLDNIQALTKPMDRLLAIQNQLEVYYANHGTVIRFPLKTALS
                     KLLGSFLRMNMYRHHMGDVAYLKRILAFLGIDYRQWGMAYVRGRVLTG"
BASE COUNT         1451 a         1442 c         1289 g         1638 t
ORIGIN      
        1 gcctcaccca caggggcatg aataatctgg ccgtgcgggt agctgagttg gccaaagaag
       61 aagggctcca actcaatctg gtcaaggtgg atgatgggca ggaacagcgc gatgagcgcc
      121 tgagcgcctt ggccggtgac aagatcaaaa ttcagtttta ccgcacggga gagaccttgg
      181 ccaagcatgt tacggaatat gatgtggtgg tcgccagtcc ctgggtgatg tcacggccgg
      241 aattttccca ccagttttcg cacttgttca ttgatgaggc aggtcagttg tcacttgcca
      301 acgctctggc catgacacac tgcgccgcca acctgatcct gttgggtgac cagagacaaa
      361 acagacaacc cttgcaggca agacatccgg ggctttccgg gttatcggtg ctggactaca
      421 tgctttctgg cttgggcact gatgtcccgg tggtccccga cgactttgga ctcttcttgg
      481 gtcagtcttt ccgcatggcc ccgcctattg ctgaatttgc cagccaggaa ttttacgatg
      541 gcagactaca gacagccgaa gtggccacgc ggcgccaact gatcgcgccc gaacgccctg
      601 tccctgttga ggttcagggc aagagattta tgtttccccc cgcgggcata gtcgccttac
      661 ccattacggg cccaatacat agccgacaaa atcctgaaga ggcgcttgcc gtgcaggcca
      721 ttttggaatc actcaaacac tttcatgttg agcaaagaaa cggtgcgggt gatgtggtgg
      781 aaagtgctcc ccttgattcc gcgcgggtct ttgtgatggc gcctttcacg gcacaggtga
      841 acgccttggc ccgtgagttg cccccagatg tgctggtgag tacggtggat ggtttgcagg
      901 ggcgcgaaaa cagtgtggtc ctgtattctc tgggagtcag tgaccctgat tcgcaacacg
      961 gggtgacatt tgcgatggac ccgcggcgca tcaacacagc gctcacgcgg gcgcaggtgc
     1021 tttttatttt actcgccaac ccgcatttgc tggacagacg ccccggcacc atcgcggaga
     1081 tgaaaaacct caatcgactg gcgatgctgt tgcgccggta cgcaccggag ccggcatcag
     1141 ggccagatag agattcagcc cgttaagacc cgaccccgca cataggccat accccattga
     1201 cgataatcaa tgcctaaaaa cgcgaggatt cttttcaaat aggccacgtc acccatatga
     1261 tgccgataca tattcattct taaaaatgat cccaggagtt ttgacagagc tgttttcaag
     1321 ggaaacctga tgaccgtacc gtgattggca taatacactt cgagctgatt ttgaatggct
     1381 aacaatctat ccatgggttt cgtcaaagct tgtatgttgt ctaatgacaa aacataaacc
     1441 agggcttcat caaggttttg tggtttgcct tctccttcaa aaatatctgt aaagcgtgac
     1501 ctcagaagtt ttttgagctc agtgccggca tttcgtgacg ggttgatgag cgctctgaat
     1561 atgaatttga ataaatctaa ttcttctttt gtgatggttg tgatttgagc gtaatccaca
     1621 cgaaccagat ttccattggc cagatcaaca ggccagttcc cctgatgccc atcaggatca
     1681 aacaccaatc tgccttcgtc aagcgcttta atttctgcat ccacaatttg ggccgcaatg
     1741 gccactcgca atgtgcgggg tctgatttta tccagctttg tatgatcaac aaacgcatac
     1801 acagaggcct gttttcgata ctctggttga atcattgttt ggatttcaag atctggtgcc
     1861 accgcagtca ctctgagtcc tgtctgtgga ctgatttcag cgtgctgata cgcccttaag
     1921 cccaaggcgg cctttgatct ttcctgctcc aagtccaatt ccacaccacc gggttttaac
     1981 gtatcatagg cctgttgcat caaattctcg atgagcatgc cataacgaag aacctgcggt
     2041 ggcctatctg gggcctttaa ttgttcgatc acttttaacc acaccttatg ttcgttttcg
     2101 atcaagccag acacatgttc tttttgaatg cgcacacaca ctctgcgcac ttcacctgtg
     2161 ctcagacgca catagacttc gaccgcataa ttgagagatc ccgagccagc cagtttcccc
     2221 acggccagta tctttatata attgtcacca aaaatctgct tgagctgtct tatgatgatc
     2281 tctctggagg gtggcagggc aaaatcaaac gaactcatga gatcatctct gagtgttcct
     2341 gtcaccagac cactggtcac cagattctga gccgcctttg cccccagagg cccgctggtt
     2401 tcaagcagtc gtctgaaaga cgagtgtccg gttgtgggat cataatttgc cagagccagg
     2461 gcaataagga tttttcgctc gccggcaggc aggacattca tgtaagtatc aaaaatttca
     2521 ctcaaggcag ggtcctcata cgctccatcc atgatgatgt ttttgacagc atcgtaaaca
     2581 aatgtgccct gcaaggtttt cagctcatca cctaaaatga cctgtagcgt cattgcctga
     2641 aacaaggccc cattctgctg atacaatgcc ttgaatcgaa taatctggtc ttgtgaatct
     2701 tcctggtctt gtttgttttg atatttttgt aagtgtggca catgaggaga gacaccgatc
     2761 aaatagcaga ttaaatcaag acgctcctgt ttggtggtga ttccttgagc cagaaaatcc
     2821 ggtgcgtcga gagcatacaa agctctcatc tgcacccagt tattttcatt caatctgagt
     2881 tcagccaaaa gccgtgtttc attttctcct ggcaagagtg cggcttcaac aaagtccaaa
     2941 acagaataac tggctggcgt tggctcggca aattgctctt gcagaagcgc acgcacatcg
     3001 cggacaatct ttcgcacttc ctgaggagtg ggcaattgcg caggatacgc cttgaggtct
     3061 tgttggtgtt gtcgaaaacc cggcaagtgc tcaatctgcc atttggcaat gtctgttttg
     3121 aaaccctcat acctgcaaag cctgatgagg cccggatata aaaatagttc tccgacctcc
     3181 tgttcaaaat ctctgaattg ccacaaatac cccagcacat cctgtatttt gtctggtaat
     3241 aggcccattt tgtttttcac tctaaacaaa agaagcctgg caacgagtgc tcggggatac
     3301 agagtcacac accttaagtc cgaaaagaaa tcgtccagag ttcttgcaaa acacctgagt
     3361 aacaactcat cactggctga tcctttggaa gaatactttc cggatttttc attgatcaat
     3421 tcttcaaccc cagcaacata ctctgcataa acagacagat ccgaaatggt tcttttgccg
     3481 gcactgacaa acgaaaaaga agacgagact cccgcttcaa tacaacggag agatgaatat
     3541 gttgtttcca agtgagctct gtcaaaggct ttccaaaatt cataagaagc ctttgtgact
     3601 gaaccatgtc gcatgagaaa ccattgaaac gattttttat aaaaccctgc tatggcatca
     3661 cgacagtcag cttgaacatc tgggtctaaa aagaatgcgg cacgcgctaa atcccagggc
     3721 ctttccaggg aaagtcgctt caaagcaagc tctgtcaatt gaccgggttg aggatcgtat
     3781 ttcgttgcct ttctttgcat ttgcgcatgg gttttctctg ctaatgtcca atggtcgatg
     3841 gggatagcca taaatatatc ataaatggca ttcagtttat cttcttcgat ttgcctgagt
     3901 ctctgcctga cctgtgcggg aatgctgctt tcttcattgt tggcgactaa catttcaacg
     3961 atgctgttca attccgtttc ttcctgcgtc cacaaagcag gttcattggt gtacacatga
     4021 gctgtttgtc taatatctaa ttgtcccttg aacacccccc agaatttctc attgttggcc
     4081 agttggatca tatcagaaaa catagcctcg cggttcaggc cctgtttggc cgcatcaggc
     4141 atcatcgctg ggatcatgag atagaccaga tgtttgattt gctcgcgtga cacccctgcg
     4201 tcaccggccg ccttaaatat gagccatacc agtctatcca ccccttgtgc cctattggca
     4261 tattgtgagt aatgaataga atacaacatc cccatttcat ctaattgttc ctgaaatcca
     4321 tgcaatgcgt ttaaccacga ttctttgttg agagaattca ggttttcaat caactctgct
     4381 gtggctttgt cgagcaagct tgtctcacat tctttaagat ctatttctgc acaatatttc
     4441 gcacctctga taagttctgc taaatttttt aaaaaatgag ggttatcatg ataataacca
     4501 tgtattttga cagcagaact acaatgtatt tctacagttt ttttgatgac attttggatg
     4561 agtgttcttg tttgagtcgg aaggcattta gaaaaataac taattaaggt ggggagacaa
     4621 tcgcgtatga aatggaaaat ttcgcaatat tgtagttccc tgttgccacc gtccctgtgt
     4681 tgaagacatt cttcaaaact tgttgctaaa tgatcggaaa tcttgttcaa gatttgttcc
     4741 agcacggctg gagaagcctt tcgatagcca taggttttta tgagcccggc taaccgaaca
     4801 ctgtcttttt ctgataacag aaaattcaaa tgatgatctt ttcttatttt tttggcgcgt
     4861 tttggggctt gaatgagcat atcatgggca atgcgcaacc ttaaaccacc cgattcaaga
     4921 ctatctacaa agagaccgtg ttttgattct aagcaaccca ccggtggttt tcggtagccc
     4981 tgatcgataa aggcatcaag cgctggtcct tgctcaaaat acaattttgc aagcctaatt
     5041 cctgcttcac atcccgcaat ctttcctttt agttctttaa tcatgtgttg gtgaaagctg
     5101 gtattcccac aagtggcgga agcactttca agctcttgga ggctctcttt attttcgtct
     5161 cttctcttca aaaagtctat gagatcccca ttctcaatgt ctacctctcg tgcttccaat
     5221 gataggtatg cttctaactg agttgtgttt tgaggtacaa aatctggtcc cagaatcgcg
     5281 tttttggctc tttcaaattc ctcatgaaga ttttgggcca gttgtaattt aacaagaata
     5341 aaccactctt cccatttact gtgactgtaa ggcaaccagg atttcatttc tgcgagtcgc
     5401 aaagcataaa catctgcaaa gctcttcacc ccacgttcaa aatccatttg cgtgcttgcc
     5461 gcgatcttgc gatacatttc atcggccaaa ccagtcacct ctaataaacg ctcaattctt
     5521 gcccgaaata cagcttcgag agcaccgtac ttaaacaaaa cagatttgac gttccccagc
     5581 acgatctgtg atcttgcgcc atcatcaagg cggtgttctg tattaccgcc ttggagcctc
     5641 accaaagcgg ccacagcacc ctccaaaacg gatattcttg ttgttaattt tgtgtgtgtc
     5701 tgcgacagac agtcccctga tccttttctt aatctggtta acacatcaat tcccgcttgt
     5761 ggatttttat ctttacggat caaacgcctc accaacgctt tggcatcggc ctcattttca
//
LOCUS       DATDUN010000871         1248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_248640, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000871 DATDUN010000000
VERSION     DATDUN010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248640"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VJC18_12085"
     CDS             <1..699
                     /locus_tag="VJC18_12085"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HLD46164.1"
                     /translation="KGKQEAGVNVIDYVEHHRDLLGKYHALCEKHNLEGSLLPMLQEY
                     IKGYGCGFFALYDHGKCCVTFQHRRIREMPPSGGRSVAAETGFNPLVAEYGKQILDAL
                     QWHGLAMVEFKVDESGRPFLMEINAKLWGSIELAISAGVNFPQMMVDLHLGKQPAIDV
                     CRKSKLRYTWPFNGDIMYGMLRPKAMCGVIKDTLCLRVKNNCQLSDIRPTIKMAGHFA
                     KTFVKYCVHGQYES"
     gene            758..>1248
                     /locus_tag="VJC18_12090"
     CDS             758..>1248
                     /locus_tag="VJC18_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46165.1"
                     /translation="MARWANAQKLDFLVLTDHDSIQGSLALKKHCNENHLNIEVPLSA
                     EYQTEYGDVIAMGIHKEIESRKYAAFVREVREQGGLLLLPHPYVGHKNINQLAEDVDM
                     IEVFNARCRNVDNIRAMDLAKKHGKGMCYAGDAHSLNELENCIVVLNQHGEFLSAVKT
                     GGMV"
BASE COUNT          337 a          221 c          336 g          354 t
ORIGIN      
        1 aagggaaagc aggaagcggg tgttaacgtg atcgattatg ttgagcatca cagggatctt
       61 ttgggcaagt atcatgcgct gtgtgaaaag cacaacttgg aagggtcttt gttaccgatg
      121 ttacaggaat acataaaggg atacgggtgt ggtttttttg cgttgtatga tcatggcaaa
      181 tgttgtgtga catttcagca ccgcagaatt cgtgaaatgc cacccagcgg aggccgcagt
      241 gtggcggctg aaactggttt caatccattg gtggcagagt acggaaaaca aattttggat
      301 gctttgcagt ggcatggttt ggccatggtc gaattcaagg tcgatgaatc aggtcgtccc
      361 tttttgatgg aaattaacgc caaactctgg ggatctattg agcttgccat ttctgcgggg
      421 gtgaatttcc cgcaaatgat ggtggatctg catttgggta agcaacctgc gatcgatgtt
      481 tgcagaaaat ccaaactcag gtacacttgg ccattcaatg gcgacatcat gtacggtatg
      541 ctcagaccaa aggccatgtg tggggtgatc aaagatactc tgtgtttgcg tgtaaaaaat
      601 aactgtcaac tgtcggatat tcgccccact ataaaaatgg cagggcactt tgccaaaaca
      661 tttgtgaagt attgtgtgca tggccagtat gaatcgtaag ggtatttttc attgtcattc
      721 agcctattcg tatgattctg tgatgtcgat tccccggttg gctcgctggg ccaatgcgca
      781 gaaactcgat ttcttagtat tgacggatca cgacagcatc caaggttcgc tggcactcaa
      841 gaagcattgc aatgaaaatc atctcaatat tgaagtgcct ctttctgccg aatatcaaac
      901 agaatatggt gatgtgattg ccatgggaat tcataaagaa attgagtctc gcaaatatgc
      961 tgcatttgtt cgagaagttc gggagcaagg agggttattg ttgttgccac acccgtatgt
     1021 aggtcataaa aatatcaatc agcttgcgga agatgtggat atgattgagg tgtttaatgc
     1081 aagatgtcga aatgtcgaca atatacgggc aatggatttg gccaaaaaac acggcaaggg
     1141 tatgtgttat gcgggtgatg ctcattcttt gaatgagttg gaaaattgca ttgttgtttt
     1201 gaatcaacac ggcgagtttc tgagtgcagt gaaaacgggc ggtatggt
//
LOCUS       DATDUN010000872         1592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_123829, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000872 DATDUN010000000
VERSION     DATDUN010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123829"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VJC18_12095"
     CDS             complement(<1..174)
                     /locus_tag="VJC18_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HLD46166.1"
                     /translation="MLSALFKPNNVAVIGASKTQTKVGYAVLSNLINGGFAGEIFPIN
                     PTADEILGHKCYPD"
     gene            complement(677..>1592)
                     /locus_tag="VJC18_12100"
     CDS             complement(677..>1592)
                     /locus_tag="VJC18_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46167.1"
                     /translation="FPTHESGVYASAEHNGSIYFGGYSIEEGGDRIPGLWVDGVWESY
                     EMMADVIYGCFVYTVSFLDDDVYGGGICFGAGFVSFPGYWLNGTWNALPKLDDARGSV
                     VSKMTISDGQVYLAGYSKNADNHRIPGYWLDGVWEGLPSLDVTKTANVDDIVVSDGSV
                     YAGGFSTNADDVIIPGYWLDGVWTALPQIDEESSGRVLDVIFHDDVFYAGGWVMDPAN
                     DDAETPGYWVDGVWTALPKIDDAKNCGVNDIQFYHDSVYASGFCANEDGFMVPGYWRD
                     GEWTALPQRSAETDAQALTILIVEPDED"
BASE COUNT          463 a          423 c          317 g          389 t
ORIGIN      
        1 atcaggataa cacttgtgac ccagaatttc gtctgcagtg gggttgatgg gaaaaatctc
       61 cccagcaaaa ccgccattga tcaaatttga aagcaccgcg tacccgactt tggtttgtgt
      121 tttggaggca ccaataacgg cgacattgtt tggtttgaaa agagcagata acatttttat
      181 ctccgtatta atgagagcat gaatgagttg cgacagagcc gcctacggcc aactgctaac
      241 ggccagaaat ctcttgctgt aagccgtagg ctgttggccg taagccatct gatgacttca
      301 cgaaccacgc agttttcggc aattacgtcc agcctatact cgaaagcaga cccatttgac
      361 gattgttttt tggatgattt tcatcaggaa ggtctttgtg cggattgtgc tactttaaaa
      421 tggcataatc cgcacaatga tagttgcgaa actcttttct atctccccac gtttatacaa
      481 ctttccatca accatctttc agaaagggat gctctgacga ttcgccatct cgctagaagt
      541 gcaggcaaac gacactaggg ttttgggcga caacgattac cagctgggta tgtttgcaga
      601 tcccagatgg tatttttaaa aatttaataa cacaaacaca agatcggcct tagaaaatcg
      661 taacgggggg ccttgctcaa tcttcgtctg gctctactat caagattgtt agggcctgag
      721 catcagtttc ggcgcttcgc tgaggtaagg ccgtccattc cccatcgcgc caataacccg
      781 gcaccatgaa gccgtcttca ttggcgcaaa aaccgctggc atatacagag tcatgataaa
      841 actggatgtc attaacgcca caatttttgg catcatcaat ttttggcaat gccgtccaga
      901 caccatcaac ccaataaccg ggtgtttccg cgtcatcatt cgccggatcc atgacccaac
      961 caccagcgta gaaaacatcg tcatgaaaaa tcacatcgag cacacgacca gaagattctt
     1021 catcaatttg tggcaaagcc gtccacacgc catcaagcca atatccggga attataacat
     1081 catctgcgtt tgttgagaat ccgccagcat aaacgctccc atccgaaaca acaatatcat
     1141 caacattcgc agtttttgtc acatccagcg aaggcaaacc ctcccaaacc ccatccaacc
     1201 aataaccggg aatccggtga ttatcggcat ttttgctata tcccgcaaga tacacctggc
     1261 catcggaaat tgtcatttta gaaacgacag aacctctggc gtcatcaagc tttggcaagg
     1321 cattccaggt tccgtttagc cagtaaccgg gaaaagaaac aaaccctgct ccaaaacaaa
     1381 taccaccacc gtaaacatcg tcatccaaaa atgaaaccgt gtatacaaaa cacccgtaaa
     1441 tcacgtctgc catcatctca taactttccc aaacaccgtc cacccaaaga cccgggatac
     1501 ggtcaccgcc ctcttcaatg ctataaccac caaagtaaat tgagccattg tgctcggctg
     1561 aggcatagac gccactttca tgcgttggga aa
//
LOCUS       DATDUN010000873         1218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_581065, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000873 DATDUN010000000
VERSION     DATDUN010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581065"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VJC18_12105"
     CDS             complement(<1..562)
                     /locus_tag="VJC18_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46168.1"
                     /translation="MKQEKKLAKTFLIFALTLFISVLVISLCPLILLQTNQPRIIEIS
                     SENLTNKLVLQGQKLMEELASETIVQKASETAVLISLMYENGMPLDTTSRQFTEVAMQ
                     KIGATGYTCVYDCKTAVMLVHPNPALVGYDMKLLKDKLPSWWKIFSPSLACVDSRGFY
                     DWEDADGVMRKKYMSMTPVKGTTLMVA"
     gene            complement(559..>1218)
                     /locus_tag="VJC18_12110"
     CDS             complement(559..>1218)
                     /locus_tag="VJC18_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLD46169.1"
                     /translation="ARVLGIRVANVPGFSTEAVAEKTMGLIILAANNLQAAHAHALQG
                     GWDYQQFRGMELRGKTLCLIGRGRIGQRVAELARVFGMRVILIGSNSSQADLHSALAQ
                     ADVISLHCPYTASTYHLIGQQEFALMQGAIFINNSRGGVVDEDALLTAIEAGHIRFAT
                     MDVFEKEPPATNHPLLQHPNVFVTPHCTWNTEEAVRNLTDSAISNIELFVRGELTEFV
                     V"
BASE COUNT          308 a          302 c          280 g          328 t
ORIGIN      
        1 cggcaaccat caacgttgtc cccttcacag gggtcatcga catgtatttt tttcgcatga
       61 cgccatcggc atcttcccag tcataaaacc cccgggaatc aacacaggcc aagctgggag
      121 aaaatatctt ccaccaactg ggcaacttgt cttttaacaa cttcatgtcg taccccacca
      181 aggcgggatt gggatgcacg agcatgacag ccgttttgca atcgtacaca caggtgtagc
      241 ccgtggcccc gatcttttgc atggccactt cggtaaattg tcgtgatgtt gtgtcgaggg
      301 gcatcccgtt ctcatacatg agtgaaatca agacggctgt ttcacttgct ttttggacaa
      361 tcgtttcaga ggccagttcc tccatgagtt tttgcccctg caacaccagt ttgttcgtga
      421 ggttttctga actgatttcg atgattctcg gttggtttgt ttgaagtaat attaaggggc
      481 aaagcgaaat gacaagaaca ctgataaaaa gcgttaaggc aaagattaaa aaagttttgg
      541 ctaatttctt ttcctgcttc atacaacgaa ttctgttaat tcacctctta caaataactc
      601 aatgtttgaa atggcactgt cagtcaggtt cctgacagcc tcttccgtgt tccaggtgca
      661 atgaggtgtg acaaaaacat ttgggtgctg caacaaggga tgattcgtgg ctggtggctc
      721 tttttcaaaa acatccatcg ttgcgaatct gatgtggcct gcttcaatgg ccgtcagcag
      781 agcatcttca tcaaccacac cacctcggga attgttgatg aaaatggcac cctgcatgag
      841 ggcaaactct tgttgaccaa tcagatggta agtgcttgcc gtatagggac aatgcaaact
      901 gatgacgtca gcttgtgcca gggcagaatg caggtctgct tgagatgaat tggacccaat
      961 caaaatgact ctcatgccaa aaacacgggc aagttctgca accctctgac caattctgcc
     1021 ccggccgata aggcaaagtg ttttgcccct taattccatc cctcgaaatt gttgataatc
     1081 ccatcccccc tggagggcat gcgcgtgggc cgcctgcaaa ttgttcgccg ccaggatgat
     1141 caatcccatt gttttttcgg caacagcttc ggtggaaaac cctggaacgt ttgctacgcg
     1201 tatgccaaga actctcgc
//
LOCUS       DATDUN010000874         1710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: bacterium isolate SMAG_U8059 k141_456400, whole genome
            shotgun sequence.
ACCESSION   DATDUN010000874 DATDUN010000000
VERSION     DATDUN010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962820
            Sequence Read Archive: SRR3989339
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (soil metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/02/2024 12:57:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,424
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 3 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456400"
                     /isolate="SMAG_U8059"
                     /isolation_source="soil"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1205)
                     /locus_tag="VJC18_12115"
     CDS             complement(66..1205)
                     /locus_tag="VJC18_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HLD46170.1"
                     /translation="MSKIIKRLFSLDLPKDKTAFLWGPRKTGKTYWIKQHLGDTKLID
                     LLKTDAFAEYASRPALLRERFRDYRGRIIIDEVQKIPAILDEVHWLIENSSASFLLTG
                     SSARKLKRGHANLLGGRAWRRIMTPLSSLEVDRFDLEDIMVSGLLPPHYLSRNPIEDL
                     RAYIADYLKEEIAAEALTRNIPAFSQFLKVAALTSSELLNYTNVAADTGVSARVVRTY
                     FDILEDTYLGFRVAPWVKSKNRRMIETEKFYLFDVGVTNYLAQRQPKKGTFEFGKSFE
                     QYILMELMTYKAYKQPDLEITFWRTAANQEVDFILNNKMTAIEIKSGQVHPHDLKGLL
                     ALSEDGAVKNKIVVSLEKERRVVQNGIQILPYQIFLEKLWEGVLV"
     gene            1461..>1710
                     /locus_tag="VJC18_12120"
     CDS             1461..>1710
                     /locus_tag="VJC18_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLD46171.1"
                     /translation="MKYFDYASEYAYFVTICTYGRMNLFGQIMDGTMVVNDFGRIVET
                     TWHDLPNHIAGIELDEFVIMPDHFHGIISIVVGAGSKPA"
BASE COUNT          430 a          395 c          400 g          485 t
ORIGIN      
        1 caaaaagccc aaagctcaaa gctcaaagaa agagggtagg ggccggtcga ttgaccggcc
       61 cgtttttaca ccaacacccc ctcccacaat ttttccaaaa atatctggta gggtaatatt
      121 tgtatgccgt tttgcaccac gcggcgttct ttttccaggc tcacaacgat tttgttttta
      181 acggctccat cttcagaaag ggccaacaat cctttgaggt cgtgtgggtg cacctgaccg
      241 gattttatct caatagcggt cattttgtta ttcaggataa aatcgacctc ctgattggcc
      301 gcggtccgcc agaaagtgat ttcgaggtct ggttgcttgt aggctttgta agtcatgagt
      361 tccatcagaa tgtattgttc gaaggatttt ccaaactcga acgtgccctt tttgggctga
      421 cgctgagcaa gataattggt gacccccaca tcaaaaaggt agaatttttc tgtttcaatc
      481 atgcgacggt ttttcgattt tacccagggt gccacacgga accccagata agtgtcttct
      541 aaaatatcaa aataggttct gacgactcgc gctgatactc ctgtgtccgc tgccacattt
      601 gtatagttaa ggagttcgct actggtcagc gccgcgactt tcaggaactg actgaacgcc
      661 gggatgttgc gcgtcagggc ctcggccgca atttcttcct tgaggtaatc ggcaatgtag
      721 gcgcgcaaat cttctatggg gttgcgggaa agataatgtg gtgggagtaa accactcacc
      781 atgatgtctt cgaggtcaaa acggtccact tctaagctcg acaatggtgt catgatcctt
      841 cgccaggcac gtccgcccaa cagattggcg tgtccgcgct tgagtttacg tgcactggaa
      901 ccggtcagca aaaatgaggc tgaagaattt tcaatcagcc aatgcacctc atcgaggatg
      961 gcgggaattt tttgcacctc atcgatgatg atacgacccc ggtaatccct aaaacgttct
     1021 ctcagcaaag ccggcctcga agcgtattcg gcaaacgcgt ctgtcttgag caagtcgatc
     1081 aactttgtat cgcccaaatg ttgtttgatc cagtaggttt tgcccgtttt gcgcggtccc
     1141 cataagaaag ccgtcttgtc ttttgggaga tctaacgaaa acagcctttt gataatctta
     1201 ctcatgagat cagtttatca tgataaactg caatgcacaa gcaatttatc gtatcttggc
     1261 gacgcacaac atttaggtgc caacaccccc taacaacaac aacctagaat gtttggccgt
     1321 tggctgtcgg ccgttgactg ttggcctttt ttttatggaa atcatgacgg tagatttttc
     1381 atattttccg caattctcgt gataacaggt gcgataaacg caatgtgtct caggattttc
     1441 ccaatcgcag atcaatcaga ttgaaatatt ttgattacgc atcagaatac gcgtattttg
     1501 tcaccatttg cacgtatggt cgcatgaatt tgtttgggca gattatggat gggacaatgg
     1561 tggtgaatga ttttggcaga attgtggaaa caacgtggca cgatttgcca aatcatattg
     1621 ccggaatcga attggacgaa tttgtcatca tgcccgatca ttttcatgga ataatatcca
     1681 ttgtcgtagg ggcgggttct aaacccgccc
//
